Lsi08G000400 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi08G000400
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	agamous-like MADS-box protein AGL12
Location	chr08: 938115 .. 942772 (-)
RNA-Seq Expression	Lsi08G000400
Synteny	Lsi08G000400

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AAGAAATGGAAGAAGTATAAAAAGGGGTTTAGAATGGGATTAGTTTGGTTTAAAGGGTGTTGAAGTTAATAGTGAGTTTTAAAGAAGGGGTAAGGTTAGAAGAATTATTGCAGCGACCTGAAGCTAGTTCAAACTAGTGGAAGGTTGTGTGCGGCGGCCAGGAATAAGAATCATCAATCAGAGAAGAAAAATGGCTCGTGGGAAGGTTCAAATGAAGAAGATTGAGAATCCAGTGCATAGACAAGTCACTTTCTGCAAGCGTCGAGCTGGGCTTCTTAAGAAGGCTAAGGAGCTCTCTGTTCTTTGCGATGCTGAAATTGGAGTTTTCATTTTCTCCGCTCATGGAAAGCTCTATGAACTCGCCACTAAAGGGTTTACCTCATCTTTCTCTTTCCAAATCTCTCATATCAATAACACATTTTCAAAAATATTTTTTTTTTTTTTTTTTTTTTTTAATATGTGGGGTGATAGAATCAAACTCTTTAATTTGCTATTTCACTGTAACGTTGTATTAGCGAATGAGTAATATACTTTGGGTAAAGTTCAAGATGCATCCATCTCCATGCATCTTTTCAAAATAAAAAATTATTTTTTTAAAGAAAAAGTTTGCCACTAACCAATTTGTGATTGGACACTTAAATGAGCTGCACAAATATGAGTGTAGCTCGAGATAGACCTAAGTACTGAATCACAAATTGCCACGTGACAAATTTTTTCTTTTTATCTTTTTGTAATCGAGGAGGATACATAGAGATGAGTACAACTTAGGTTGTATCTAAGTGTTACCCGAATATTAATTTAATTTGGTGGGCATAGAATATATATGTACATATTCTTTTTGAGTATTGGTTTGATTTGTGTGTCTCATTTTATGTTGGAGGATATAGAACCATGCAAGAGCTTATTGAAAGGTATATGAAGCACACCAATGGGAATCAGCCACCAGACCCACCTATTCATCACCAAACTCTGGTGCACACTCTCTCTCCATTTCTTTTTAATTAATTATATTTTTCCTTATTTTAATTTGATGTGACAAAAGGGTCAATTGATTTTTCTATGGATAAGTACTATTAATTACAAATGAAATCAAATTTAGTCCCACTATAATTCTTTTAAAATTGTTAATTTAAAATAAGAATACAAAGAAAAGAAAAGAAAAGGAAAGGAAGGCATCTCCCAAACAAAAATTCCATAGTTTTTTTAAGTGAATTTGTTTACAAGTGATATGCTGAATCTGATGCTACTTCTATATATATATTCTCAATTATTCCCATTAATGTGAATTCTGACCATTGGAATTAATTAAAGGAGAGATTGCTCATATAAATAGTTATTCAAATACATATATACATATTTATTAGGAGACTTTTTAAAAATAAAAAAATTAAAGAAACTATTTACACAAAATAGCAAAATTTTTAGATAGTTAGATAGATGATGATAGAAGTCTATCAGGGTCTATCGGTGATAGAAATGATAGAAGTCTATCATTGATAGATGCTGATAGAAGTCTATCAATGTCTATCAGTGATCTTTTTGCTATTTTTTTGTAAATAGTTTAACATTTTTTCTATTTATGAAAATTTCTCTATTTATTATGTTTTTGAGGAAAAAAAGAATAATTATGTTCTGTAATTGATTTTAATTAATAATCCTAACCTAGATTTTAAAACTAAACAATGAACGACTTTAAAACCAAGATAACAAATATAATACTAAATATTTGAATTTCTAACTAAATTTAAAACACAAAAACTATTTTATTTTTATTAGAAAAATTATTATAAATAAAAAAATATCAAACTATTTATAAATATTGAAAAATTTCACTATCTATCCGCAATAGAAGTTCATCGTTGTCTATCGTTCAAGAGATAGAAGTCTATTGTAGTATATGTTGATAGACAGTGAATTTTTTTTATATTTGTAAATAGTTTGGTTCATTTTTCTATATTTGAAAATAACCATTTTTTTTAATTATTTGGTTTTTTTTATTCTTTGAAAATTGTGCTCGTTTTTCATAATTCTTAATCATTAGTTGATATTTCATATATAAATGTTTTAATTCTCAATCAAATTTCAAAAACAAAAATATTTTTTAAAAGGAAGATTTTTAAAAATAGAAAAATATGGAAAATTATTTACACAAAATAGCAAAATTTTTAGATAGTTGTGATAGATACTAATAGAAGTCTATTAGGGTCTATCAGTGATAGAAATGATAGAAGTCTATCACCGATAGAGGTTGATAGAAGTCTATCAATGTCTATCAGTGTTTCTTTTTTTTTTTTTTTTTCTATTTTCTGTAAATAGTTTGACATTTTTTCTATCTGTGAAAATTTTCCTTTTTAAAAACTAATTTTTTTTCTTAGTTTTCAAATTTTAGCTTGAATTTTAAAAATATTCCTCAACAAAATACAAAGACCCAAATAGGTGTAAGGGGGTGTTTTTCAAAACTTGGCTGGATTGGAAATACTCCTAAAATGTACATAAAAAAATAGGGAATTTCTAAAAATAAAAAAATAAAGAAAACTATTCACACAAAATAGTAAAATTTTTAGATGGTTGTGATAGACGTTGATAGAAGTCTATTAGGGTCTATCGGTGATAGAAATGATAGAAGTCTATCAATGTCTATCATTGTTTTTTTTTTATATATATTTTCTGTAAATAGTTTAACATTTTTTCTATATATGAAAATTTTCCTGCAAAAATTAAGAAATTAATACATGAAAATAGTGTTTATAAGTTGATTTTTAAAATCCAAATAATCATAGGGACCTTAGTATTTTGTATTTGTTTTTTGAATGAATAGAAAATATATTTTAGACTGTTTTTGGTTTTATCTAGGAAGTAAAGGAGGAGATTATCAGGCTGAAACAAGAAATTGAAGTCCTGAAGGGTCTCAGGTAAGACATAATTATTAACTTTCGTGATATATATATATATATTGACTTTATAATTAAATTTCCTACTCATATAAAAATTAAATCTACAGTTTAGTTAACCTATCATCACACTTGCTTGTGTTCTAATTTTTTAAAAGATAACATGTCAGCATGTCATATATCGTCGTGTCATGTAATGTTTATGTGTTGTATTCGTATTCGTGCTTTTCTTAATACAAACACAACTCTAATATTTTGGGAGTTCCTGAGGCAATATAATTTTAAAATAAATAAATGTAAATGTAGGAATGCTTTGGGAGGAGGAGGAGCTGAAGGAATAATGAGATTGGATGAGCTTGATATGTTTGAGAAGCAACTTGAGATTTGGATTTGTCAAGTGCGTTCTACAAAGGTACACTTTGCTTCCAAAAAAAAAAAATTAAACTTTCTTTTCTTCACTATGACAATTTTGAGATTAATATGTGTTGCTATATGCAATGAAATTAATTGACATTCATCATGCAGATGAAGTTCATGTGTGAAGAGATTGAAGCACTAAGGAACCAGGTTTGACTTTTTTTTTACTCTTTCAAATATATCTAATAAAATTTAGGGTGGTTTACAAATATAAAAAAAAAAAAAAAAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTAGCTAAATTATTTATAAATATAGAAAATTTTTATTGTCTATCAACAATAGACCTTGATAGTCAGCGATAGAAGTCTATCGCGGTCTGATAGACATTGAAATTTTTCTATATTTATAAATAATTTAATTTTTTTTTATAATAATTTTCCTTAAAAAAATTTATGTTATGTGCAAGTTTACGAAATTTTTTTAAAAAATAGGTCTTAGGTCATCGTCACTAGTTTCCCATTAGATAGGCTCTTATATTTGTTTACTAATTTTGTAATATTAATTATATTGACAGCCAATGTATTCTAAAATTTAGATGTTTTGATTTCAACTCACATGAACTATTTGAGAAATCAATTTCATATTTTTGGGATAGCTGTGTAACAACGTTCACATAGAAAGAAAATTGAGCATAGCTTAGCGTATAATGCACTAATTACCATATAAAAATTCGATTATTTTGATTTCTACTCCAAAATTATTAAAAGAACATTTCATATTCTTAGTCTTATATTATGTCGAAATTAAATATGTTTTTTTATTTTTAATTATTGGGTTCGAATGGTAATATTAATCAAATCTAAACTTTCTTTCTATTGGAAGGAATTGATATTGAAAGCTGCCAACAAGTATCTTCATGATAAGGTACTAAGGTTGAGAATCTATTATTGGAAAAATCAAACAACTTCACAATCTTAATAAGATAGATGAATTACTTTTCTTATTGTCAATTAATTTTGAAATAAAATTGAAATCTGATTACATATGTAAATCAATATCGAGCTAATTAGGGATGTCCAATAAATATATATATATATATATATGTGTGTGTATTTTTTTTTAATTTGTAAGGGAATGAAACATTAAATCTGTCCCTTCTATTGTGGTTTTGCAGATGGAGGAAAACATTAGCAACACGACTAACATTGAACCTGTGAATATTACTAACTGTCAATATCCACTAACTATACAGGATGAGTTATTTCAGCTTTGCACTTAAAGTTAGAAGCTAGAAAAAACATATGTTATCTCTTTTAATGT

mRNA sequence

AAGAAATGGAAGAAGTATAAAAAGGGGTTTAGAATGGGATTAGTTTGGTTTAAAGGGTGTTGAAGTTAATAGTGAGTTTTAAAGAAGGGGTAAGGTTAGAAGAATTATTGCAGCGACCTGAAGCTAGTTCAAACTAGTGGAAGGTTGTGTGCGGCGGCCAGGAATAAGAATCATCAATCAGAGAAGAAAAATGGCTCGTGGGAAGGTTCAAATGAAGAAGATTGAGAATCCAGTGCATAGACAAGTCACTTTCTGCAAGCGTCGAGCTGGGCTTCTTAAGAAGGCTAAGGAGCTCTCTGTTCTTTGCGATGCTGAAATTGGAGTTTTCATTTTCTCCGCTCATGGAAAGCTCTATGAACTCGCCACTAAAGGGAATGCTTTGGGAGGAGGAGGAGCTGAAGGAATAATGAGATTGGATGAGCTTGATATGTTTGAGAAGCAACTTGAGATTTGGATTTGTCAAGTGCGTTCTACAAAGATGAAGTTCATGTGTGAAGAGATTGAAGCACTAAGGAACCAGATGGAGGAAAACATTAGCAACACGACTAACATTGAACCTGTGAATATTACTAACTGTCAATATCCACTAACTATACAGGATGAGTTATTTCAGCTTTGCACTTAAAGTTAGAAGCTAGAAAAAACATATGTTATCTCTTTTAATGT

Coding sequence (CDS)

ATGGCTCGTGGGAAGGTTCAAATGAAGAAGATTGAGAATCCAGTGCATAGACAAGTCACTTTCTGCAAGCGTCGAGCTGGGCTTCTTAAGAAGGCTAAGGAGCTCTCTGTTCTTTGCGATGCTGAAATTGGAGTTTTCATTTTCTCCGCTCATGGAAAGCTCTATGAACTCGCCACTAAAGGGAATGCTTTGGGAGGAGGAGGAGCTGAAGGAATAATGAGATTGGATGAGCTTGATATGTTTGAGAAGCAACTTGAGATTTGGATTTGTCAAGTGCGTTCTACAAAGATGAAGTTCATGTGTGAAGAGATTGAAGCACTAAGGAACCAGATGGAGGAAAACATTAGCAACACGACTAACATTGAACCTGTGAATATTACTAACTGTCAATATCCACTAACTATACAGGATGAGTTATTTCAGCTTTGCACTTAA

Protein sequence

MARGKVQMKKIENPVHRQVTFCKRRAGLLKKAKELSVLCDAEIGVFIFSAHGKLYELATKGNALGGGGAEGIMRLDELDMFEKQLEIWICQVRSTKMKFMCEEIEALRNQMEENISNTTNIEPVNITNCQYPLTIQDELFQLCT

Homology

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BLAST of Lsi08G000400 vs. ExPASy Swiss-Prot
Match: Q38841 (Agamous-like MADS-box protein AGL12 OS=Arabidopsis thaliana OX=3702 GN=AGL12 PE=1 SV=2)

HSP 1 Score: 143.7 bits (361), Expect = 1.7e-33
Identity = 92/210 (43.81%), Postives = 109/210 (51.90%), Query Frame = 0


Query: 1   MARGKVQMKKIENPVHRQVTFCKRRAGLLKKAKELSVLCDAEIGVFIFSAHGKLYELATK 60
           MARGK+Q+K+IENPVHRQVTFCKRR GLLKKAKELSVLCDAEIGV IFS  GKL+ELATK
Sbjct: 1   MARGKIQLKRIENPVHRQVTFCKRRTGLLKKAKELSVLCDAEIGVVIFSPQGKLFELATK 60

Query: 61  G----------NALGG-------------------------------------------- 120
           G             GG                                            
Sbjct: 61  GTMEGMIDKYMKCTGGGRGSSSATFTAQEQLQPPNLDPKDEINVLKQEIEMLQKGISYMF 120

Query: 121 GGAEGIMRLDELDMFEKQLEIWICQVRSTKMKFMCEEIEALRNQ-----------MEENI 142
           GG +G M L+EL + EK LE WI Q+RS KM  M +EI++LRN+           +E+  
Sbjct: 121 GGGDGAMNLEELLLLEKHLEYWISQIRSAKMDVMLQEIQSLRNKEGVLKNTNKYLLEKIE 180

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BLAST of Lsi08G000400 vs. ExPASy Swiss-Prot
Match: Q2QW55 (MADS-box transcription factor 33 OS=Oryza sativa subsp. japonica OX=39947 GN=MADS33 PE=2 SV=2)

HSP 1 Score: 132.5 bits (332), Expect = 3.8e-30
Identity = 76/156 (48.72%), Postives = 91/156 (58.33%), Query Frame = 0


Query: 1   MARGKVQMKKIENPVHRQVTFCKRRAGLLKKAKELSVLCDAEIGVFIFSAHGKLYELATK 60
           M RGKVQM++IENPVHRQVTFCKRR GLLKKA+ELSVLCDA++GV IFS+ GKL+ELAT 
Sbjct: 1   MVRGKVQMRRIENPVHRQVTFCKRRGGLLKKARELSVLCDADVGVIIFSSQGKLHELATN 60

Query: 61  G-----------NALGG-----------------------------------GGAEGIMR 111
           G           N  GG                                   GG  G M 
Sbjct: 61  GNMHNLVERYQSNVAGGQMEPGALQRQQVAEQGIFLLREEIDLLQRGLRSTYGGGAGEMT 120

[Close]

BLAST of Lsi08G000400 vs. ExPASy Swiss-Prot
Match: A2YQK9 (MADS-box transcription factor 26 OS=Oryza sativa subsp. indica OX=39946 GN=MADS26 PE=2 SV=2)

HSP 1 Score: 128.3 bits (321), Expect = 7.2e-29
Identity = 72/155 (46.45%), Postives = 93/155 (60.00%), Query Frame = 0


Query: 1   MARGKVQMKKIENPVHRQVTFCKRRAGLLKKAKELSVLCDAEIGVFIFSAHGKLYELATK 60
           MARGKVQ+++IENPVHRQVTFCKRRAGLLKKA+ELS+LC+A+IG+ IFSAHGKLY+LAT 
Sbjct: 1   MARGKVQLRRIENPVHRQVTFCKRRAGLLKKARELSILCEADIGIIIFSAHGKLYDLATT 60

Query: 61  G-----------------NALG----------------------------GGGAEGIMRL 111
           G                 NA G                            G  A   M +
Sbjct: 61  GTMEELIERYKSASGEQANACGDQRMDPKQEAMVLKQEINLLQKGLRYIYGNRANEHMTV 120

[Close]

BLAST of Lsi08G000400 vs. ExPASy Swiss-Prot
Match: Q0J8G8 (MADS-box transcription factor 26 OS=Oryza sativa subsp. japonica OX=39947 GN=MADS26 PE=2 SV=1)

HSP 1 Score: 128.3 bits (321), Expect = 7.2e-29
Identity = 72/155 (46.45%), Postives = 93/155 (60.00%), Query Frame = 0


Query: 1   MARGKVQMKKIENPVHRQVTFCKRRAGLLKKAKELSVLCDAEIGVFIFSAHGKLYELATK 60
           MARGKVQ+++IENPVHRQVTFCKRRAGLLKKA+ELS+LC+A+IG+ IFSAHGKLY+LAT 
Sbjct: 1   MARGKVQLRRIENPVHRQVTFCKRRAGLLKKARELSILCEADIGIIIFSAHGKLYDLATT 60

Query: 61  G-----------------NALG----------------------------GGGAEGIMRL 111
           G                 NA G                            G  A   M +
Sbjct: 61  GTMEELIERYKSASGEQANACGDQRMDPKQEAMVLKQEINLLQKGLRYIYGNRANEHMTV 120

[Close]

BLAST of Lsi08G000400 vs. ExPASy Swiss-Prot
Match: Q8LLR2 (Agamous-like MADS-box protein MADS2 OS=Vitis vinifera OX=29760 GN=MADS2 PE=2 SV=2)

HSP 1 Score: 96.3 bits (238), Expect = 3.0e-19
Identity = 63/179 (35.20%), Postives = 90/179 (50.28%), Query Frame = 0


Query: 1   MARGKVQMKKIENPVHRQVTFCKRRAGLLKKAKELSVLCDAEIGVFIFSAHGKLYELATK 60
           M RG+V++K+IEN ++RQVTF KRR GLLKKA ELSVLCDAE+ + IFS  GKLYE  + 
Sbjct: 1   MGRGRVELKRIENKINRQVTFAKRRNGLLKKAYELSVLCDAEVALIIFSTRGKLYEFCSS 60

Query: 61  GNAL---------GGGGAE--------------------------------------GIM 120
            + L           G  E                                      G +
Sbjct: 61  SSMLKTLERYQKCSYGAVEVSRPSKELEQSSYREYLKLKSKFESLQRTQRNLLGEDLGPL 120

Query: 121 RLDELDMFEKQLEIWICQVRSTKMKFMCEEIEALRNQ----MEENISNTTNIEPVNITN 129
              EL+  E+QLE  + QVRSTK +FM +++  L+N+    +E N + T  ++ +++ N
Sbjct: 121 NTKELEQLERQLETSLKQVRSTKTQFMLDQLSDLQNKEQVLVESNKALTRKLDEISVKN 179

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BLAST of Lsi08G000400 vs. ExPASy TrEMBL
Match: A0A0A0KW77 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G650620 PE=4 SV=1)

HSP 1 Score: 245.0 bits (624), Expect = 2.0e-61
Identity = 138/207 (66.67%), Postives = 142/207 (68.60%), Query Frame = 0


Query: 1   MARGKVQMKKIENPVHRQVTFCKRRAGLLKKAKELSVLCDAEIGVFIFSAHGKLYELATK 60
           MARGKVQMK+IENPVHRQVTFCKRRAGLLKKAKELSVLCDAEIG+FIFSAHGKLYELATK
Sbjct: 1   MARGKVQMKRIENPVHRQVTFCKRRAGLLKKAKELSVLCDAEIGLFIFSAHGKLYELATK 60

Query: 61  G------------------------------------------------NALGGGGAEGI 120
           G                                                NALGGGG EGI
Sbjct: 61  GTMQGLIERYMKHTNGNQPPDPPIHHQTLEVKEEIIRLKQEIEVLKGLRNALGGGGGEGI 120

Query: 121 MRLDELDMFEKQLEIWICQVRSTKMKFMCEEIEALRNQ--------------ME-ENISN 145
           M LDELD+FEKQLEIWICQVRSTKMKFMCEEIEALRNQ              ME ENISN
Sbjct: 121 MTLDELDIFEKQLEIWICQVRSTKMKFMCEEIEALRNQEFILTAANKYLHDKMEAENISN 180

[Close]

BLAST of Lsi08G000400 vs. ExPASy TrEMBL
Match: A0A6J1IA69 (agamous-like MADS-box protein AGL12 OS=Cucurbita maxima OX=3661 GN=LOC111472603 PE=4 SV=1)

HSP 1 Score: 241.5 bits (615), Expect = 2.2e-60
Identity = 135/206 (65.53%), Postives = 138/206 (66.99%), Query Frame = 0


Query: 1   MARGKVQMKKIENPVHRQVTFCKRRAGLLKKAKELSVLCDAEIGVFIFSAHGKLYELATK 60
           MARGKVQ+K+IENPVHRQVTFCKRRAGLLKKAKELSVLCDAEIGVFIFSAHGKLYELATK
Sbjct: 1   MARGKVQLKRIENPVHRQVTFCKRRAGLLKKAKELSVLCDAEIGVFIFSAHGKLYELATK 60

Query: 61  G------------------------------------------------NALGGGGAEGI 120
           G                                                NALGGGG EGI
Sbjct: 61  GTMQGLIERYMNHTNGSQPPEPRIDNQTLEVKEEVNRLKQEIEVLKGLRNALGGGGVEGI 120

Query: 121 MRLDELDMFEKQLEIWICQVRSTKMKFMCEEIEALRNQ--------------MEENISNT 145
             LDELDM EKQLEIWICQVRSTKMKFMCEEI+ALRNQ              MEENI NT
Sbjct: 121 RTLDELDMLEKQLEIWICQVRSTKMKFMCEEIDALRNQELILKAANKYLHDKMEENICNT 180

[Close]

BLAST of Lsi08G000400 vs. ExPASy TrEMBL
Match: A0A6J1ECC1 (agamous-like MADS-box protein AGL12 OS=Cucurbita moschata OX=3662 GN=LOC111432780 PE=4 SV=1)

HSP 1 Score: 241.5 bits (615), Expect = 2.2e-60
Identity = 135/206 (65.53%), Postives = 138/206 (66.99%), Query Frame = 0


Query: 1   MARGKVQMKKIENPVHRQVTFCKRRAGLLKKAKELSVLCDAEIGVFIFSAHGKLYELATK 60
           MARGKVQ+K+IENPVHRQVTFCKRRAGLLKKAKELSVLCDAEIGVFIFSAHGKLYELATK
Sbjct: 1   MARGKVQLKRIENPVHRQVTFCKRRAGLLKKAKELSVLCDAEIGVFIFSAHGKLYELATK 60

Query: 61  G------------------------------------------------NALGGGGAEGI 120
           G                                                NALGGGG EGI
Sbjct: 61  GTMQGLIERYMNHTNGSQPPEPPIDNQTLEVKEEVNRLKQEIEVLKGLRNALGGGGVEGI 120

Query: 121 MRLDELDMFEKQLEIWICQVRSTKMKFMCEEIEALRNQ--------------MEENISNT 145
             LDELDM EKQLEIWICQVRSTKMKFMCEEI+ALRNQ              MEENI NT
Sbjct: 121 RTLDELDMLEKQLEIWICQVRSTKMKFMCEEIDALRNQELILKAANKYLHDKMEENICNT 180

[Close]

BLAST of Lsi08G000400 vs. ExPASy TrEMBL
Match: A0A6J1D898 (agamous-like MADS-box protein AGL12 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111018277 PE=4 SV=1)

HSP 1 Score: 229.6 bits (584), Expect = 8.5e-57
Identity = 130/192 (67.71%), Postives = 134/192 (69.79%), Query Frame = 0


Query: 1   MARGKVQMKKIENPVHRQVTFCKRRAGLLKKAKELSVLCDAEIGVFIFSAHGKLYELATK 60
           MARGKVQMK+IENPVHRQVTFCKRRAGLLKKAKELSVLCDAEIG+FIFSAHGKLYELATK
Sbjct: 1   MARGKVQMKRIENPVHRQVTFCKRRAGLLKKAKELSVLCDAEIGLFIFSAHGKLYELATK 60

Query: 61  GNALG-----------------------------------------------GGGAEGIM 120
           G   G                                               GGGAEG M
Sbjct: 61  GTMQGLIERYMNHTSRTQPPDPSIENQTLEIKEEVNRLKQEIEVQKGLRNAFGGGAEG-M 120

Query: 121 RLDELDMFEKQLEIWICQVRSTKMKFMCEEIEALRNQMEENISNTTNIEPV-NITNCQYP 145
            LDELDMFEKQLEIWICQVRSTKMKFMC+EIEAL+NQMEENI  TTNIE V N TN QYP
Sbjct: 121 TLDELDMFEKQLEIWICQVRSTKMKFMCQEIEALKNQMEENIGYTTNIEAVNNNTNFQYP 180

[Close]

BLAST of Lsi08G000400 vs. ExPASy TrEMBL
Match: A0A1S4E2G4 (agamous-like MADS-box protein AGL12 OS=Cucumis melo OX=3656 GN=LOC103498765 PE=4 SV=1)

HSP 1 Score: 221.1 bits (562), Expect = 3.0e-54
Identity = 124/184 (67.39%), Postives = 127/184 (69.02%), Query Frame = 0


Query: 1   MARGKVQMKKIENPVHRQVTFCKRRAGLLKKAKELSVLCDAEIGVFIFSAHGKLYELATK 60
           MARGKVQMK+IENPVHRQVTFCKRRAGLLKKAKELSVLCDAEIG+FIFSAHGKLYELATK
Sbjct: 1   MARGKVQMKRIENPVHRQVTFCKRRAGLLKKAKELSVLCDAEIGLFIFSAHGKLYELATK 60

Query: 61  G------------------------------------------------NALGGGGAEGI 120
           G                                                NALGGGG EGI
Sbjct: 61  GTMQGLIERYMKHTNGNQPPDPPIHHQTSEAKEEIIRLKQEIEVLKGLRNALGGGGGEGI 120

Query: 121 MRLDELDMFEKQLEIWICQVRSTKMKFMCEEIEALRNQ-------MEENISNTTNIEPVN 130
           MRLDELDMFEKQLEIWICQVRSTKMKFM EEIEALRNQ       MEENISNTTN+EPVN
Sbjct: 121 MRLDELDMFEKQLEIWICQVRSTKMKFMSEEIEALRNQVGCLINFMEENISNTTNMEPVN 180

[Close]

BLAST of Lsi08G000400 vs. NCBI nr
Match: XP_038891170.1 (agamous-like MADS-box protein AGL12 [Benincasa hispida])

HSP 1 Score: 249.6 bits (636), Expect = 1.6e-62
Identity = 139/205 (67.80%), Postives = 141/205 (68.78%), Query Frame = 0


Query: 1   MARGKVQMKKIENPVHRQVTFCKRRAGLLKKAKELSVLCDAEIGVFIFSAHGKLYELATK 60
           MARGKVQMK+IENPVHRQVTFCKRRAGLLKKAKELSVLCDAEIGVFIFSAHGKLYELATK
Sbjct: 1   MARGKVQMKRIENPVHRQVTFCKRRAGLLKKAKELSVLCDAEIGVFIFSAHGKLYELATK 60

Query: 61  G------------------------------------------------NALGGGGAEGI 120
           G                                                NALGGGGAEGI
Sbjct: 61  GTMQGLIERYMKHTNGNQPPDPPIDHQTLEVKEEIIRLKQEIEVLKGLRNALGGGGAEGI 120

Query: 121 MRLDELDMFEKQLEIWICQVRSTKMKFMCEEIEALRNQ--------------MEENISNT 144
           M LDELDMFEKQLEIWICQVRSTKMKFMCEEIEALRNQ              MEENISNT
Sbjct: 121 MTLDELDMFEKQLEIWICQVRSTKMKFMCEEIEALRNQELILKAANKYLHDKMEENISNT 180

[Close]

BLAST of Lsi08G000400 vs. NCBI nr
Match: XP_011656111.1 (agamous-like MADS-box protein AGL12 [Cucumis sativus] >KGN52697.1 hypothetical protein Csa_008811 [Cucumis sativus])

HSP 1 Score: 245.0 bits (624), Expect = 4.0e-61
Identity = 138/207 (66.67%), Postives = 142/207 (68.60%), Query Frame = 0


Query: 1   MARGKVQMKKIENPVHRQVTFCKRRAGLLKKAKELSVLCDAEIGVFIFSAHGKLYELATK 60
           MARGKVQMK+IENPVHRQVTFCKRRAGLLKKAKELSVLCDAEIG+FIFSAHGKLYELATK
Sbjct: 1   MARGKVQMKRIENPVHRQVTFCKRRAGLLKKAKELSVLCDAEIGLFIFSAHGKLYELATK 60

Query: 61  G------------------------------------------------NALGGGGAEGI 120
           G                                                NALGGGG EGI
Sbjct: 61  GTMQGLIERYMKHTNGNQPPDPPIHHQTLEVKEEIIRLKQEIEVLKGLRNALGGGGGEGI 120

Query: 121 MRLDELDMFEKQLEIWICQVRSTKMKFMCEEIEALRNQ--------------ME-ENISN 145
           M LDELD+FEKQLEIWICQVRSTKMKFMCEEIEALRNQ              ME ENISN
Sbjct: 121 MTLDELDIFEKQLEIWICQVRSTKMKFMCEEIEALRNQEFILTAANKYLHDKMEAENISN 180

[Close]

BLAST of Lsi08G000400 vs. NCBI nr
Match: XP_022973991.1 (agamous-like MADS-box protein AGL12 [Cucurbita maxima])

HSP 1 Score: 241.5 bits (615), Expect = 4.5e-60
Identity = 135/206 (65.53%), Postives = 138/206 (66.99%), Query Frame = 0


Query: 1   MARGKVQMKKIENPVHRQVTFCKRRAGLLKKAKELSVLCDAEIGVFIFSAHGKLYELATK 60
           MARGKVQ+K+IENPVHRQVTFCKRRAGLLKKAKELSVLCDAEIGVFIFSAHGKLYELATK
Sbjct: 1   MARGKVQLKRIENPVHRQVTFCKRRAGLLKKAKELSVLCDAEIGVFIFSAHGKLYELATK 60

Query: 61  G------------------------------------------------NALGGGGAEGI 120
           G                                                NALGGGG EGI
Sbjct: 61  GTMQGLIERYMNHTNGSQPPEPRIDNQTLEVKEEVNRLKQEIEVLKGLRNALGGGGVEGI 120

Query: 121 MRLDELDMFEKQLEIWICQVRSTKMKFMCEEIEALRNQ--------------MEENISNT 145
             LDELDM EKQLEIWICQVRSTKMKFMCEEI+ALRNQ              MEENI NT
Sbjct: 121 RTLDELDMLEKQLEIWICQVRSTKMKFMCEEIDALRNQELILKAANKYLHDKMEENICNT 180

[Close]

BLAST of Lsi08G000400 vs. NCBI nr
Match: XP_022925494.1 (agamous-like MADS-box protein AGL12 [Cucurbita moschata] >XP_023536341.1 agamous-like MADS-box protein AGL12 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 241.5 bits (615), Expect = 4.5e-60
Identity = 135/206 (65.53%), Postives = 138/206 (66.99%), Query Frame = 0


Query: 1   MARGKVQMKKIENPVHRQVTFCKRRAGLLKKAKELSVLCDAEIGVFIFSAHGKLYELATK 60
           MARGKVQ+K+IENPVHRQVTFCKRRAGLLKKAKELSVLCDAEIGVFIFSAHGKLYELATK
Sbjct: 1   MARGKVQLKRIENPVHRQVTFCKRRAGLLKKAKELSVLCDAEIGVFIFSAHGKLYELATK 60

Query: 61  G------------------------------------------------NALGGGGAEGI 120
           G                                                NALGGGG EGI
Sbjct: 61  GTMQGLIERYMNHTNGSQPPEPPIDNQTLEVKEEVNRLKQEIEVLKGLRNALGGGGVEGI 120

Query: 121 MRLDELDMFEKQLEIWICQVRSTKMKFMCEEIEALRNQ--------------MEENISNT 145
             LDELDM EKQLEIWICQVRSTKMKFMCEEI+ALRNQ              MEENI NT
Sbjct: 121 RTLDELDMLEKQLEIWICQVRSTKMKFMCEEIDALRNQELILKAANKYLHDKMEENICNT 180

[Close]

BLAST of Lsi08G000400 vs. NCBI nr
Match: KAG6592380.1 (Agamous-like MADS-box protein AGL12, partial [Cucurbita argyrosperma subsp. sororia] >KAG7025197.1 Agamous-like MADS-box protein AGL12 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 241.5 bits (615), Expect = 4.5e-60
Identity = 135/206 (65.53%), Postives = 138/206 (66.99%), Query Frame = 0


Query: 1   MARGKVQMKKIENPVHRQVTFCKRRAGLLKKAKELSVLCDAEIGVFIFSAHGKLYELATK 60
           MARGKVQ+K+IENPVHRQVTFCKRRAGLLKKAKELSVLCDAEIGVFIFSAHGKLYELATK
Sbjct: 1   MARGKVQLKRIENPVHRQVTFCKRRAGLLKKAKELSVLCDAEIGVFIFSAHGKLYELATK 60

Query: 61  G------------------------------------------------NALGGGGAEGI 120
           G                                                NALGGGG EGI
Sbjct: 61  GTMQGLIERYMNHTNGSQPPEPPMDNQTLEVKEEVNRLKQEIEVLKGLRNALGGGGVEGI 120

Query: 121 MRLDELDMFEKQLEIWICQVRSTKMKFMCEEIEALRNQ--------------MEENISNT 145
             LDELDM EKQLEIWICQVRSTKMKFMCEEI+ALRNQ              MEENI NT
Sbjct: 121 RTLDELDMLEKQLEIWICQVRSTKMKFMCEEIDALRNQELILKAANKYLHDKMEENICNT 180

[Close]

BLAST of Lsi08G000400 vs. TAIR 10
Match: AT1G71692.1 (AGAMOUS-like 12 )

HSP 1 Score: 143.7 bits (361), Expect = 1.2e-34
Identity = 92/210 (43.81%), Postives = 109/210 (51.90%), Query Frame = 0


Query: 1   MARGKVQMKKIENPVHRQVTFCKRRAGLLKKAKELSVLCDAEIGVFIFSAHGKLYELATK 60
           MARGK+Q+K+IENPVHRQVTFCKRR GLLKKAKELSVLCDAEIGV IFS  GKL+ELATK
Sbjct: 1   MARGKIQLKRIENPVHRQVTFCKRRTGLLKKAKELSVLCDAEIGVVIFSPQGKLFELATK 60

Query: 61  G----------NALGG-------------------------------------------- 120
           G             GG                                            
Sbjct: 61  GTMEGMIDKYMKCTGGGRGSSSATFTAQEQLQPPNLDPKDEINVLKQEIEMLQKGISYMF 120

Query: 121 GGAEGIMRLDELDMFEKQLEIWICQVRSTKMKFMCEEIEALRNQ-----------MEENI 142
           GG +G M L+EL + EK LE WI Q+RS KM  M +EI++LRN+           +E+  
Sbjct: 121 GGGDGAMNLEELLLLEKHLEYWISQIRSAKMDVMLQEIQSLRNKEGVLKNTNKYLLEKIE 180

[Close]

BLAST of Lsi08G000400 vs. TAIR 10
Match: AT5G15800.1 (K-box region and MADS-box transcription factor family protein )

HSP 1 Score: 88.2 bits (217), Expect = 5.9e-18
Identity = 57/157 (36.31%), Postives = 78/157 (49.68%), Query Frame = 0


Query: 1   MARGKVQMKKIENPVHRQVTFCKRRAGLLKKAKELSVLCDAEIGVFIFSAHGKLYELATK 60
           M RG+V++K+IEN ++RQVTF KRR GLLKKA ELSVLCDAE+ + IFS  GKLYE  + 
Sbjct: 1   MGRGRVELKRIENKINRQVTFAKRRNGLLKKAYELSVLCDAEVALIIFSNRGKLYEFCSS 60

Query: 61  GNAL---------GGGGAE--------------------------------------GIM 111
            N L           G  E                                      G +
Sbjct: 61  SNMLKTLDRYQKCSYGSIEVNNKPAKELENSYREYLKLKGRYENLQRQQRNLLGEDLGPL 120

[Close]

BLAST of Lsi08G000400 vs. TAIR 10
Match: AT5G15800.2 (K-box region and MADS-box transcription factor family protein )

HSP 1 Score: 88.2 bits (217), Expect = 5.9e-18
Identity = 57/157 (36.31%), Postives = 78/157 (49.68%), Query Frame = 0


Query: 1   MARGKVQMKKIENPVHRQVTFCKRRAGLLKKAKELSVLCDAEIGVFIFSAHGKLYELATK 60
           M RG+V++K+IEN ++RQVTF KRR GLLKKA ELSVLCDAE+ + IFS  GKLYE  + 
Sbjct: 1   MGRGRVELKRIENKINRQVTFAKRRNGLLKKAYELSVLCDAEVALIIFSNRGKLYEFCSS 60

Query: 61  GNAL---------GGGGAE--------------------------------------GIM 111
            N L           G  E                                      G +
Sbjct: 61  SNMLKTLDRYQKCSYGSIEVNNKPAKELENSYREYLKLKGRYENLQRQQRNLLGEDLGPL 120

[Close]

BLAST of Lsi08G000400 vs. TAIR 10
Match: AT2G03710.1 (K-box region and MADS-box transcription factor family protein )

HSP 1 Score: 86.3 bits (212), Expect = 2.2e-17
Identity = 55/159 (34.59%), Postives = 78/159 (49.06%), Query Frame = 0


Query: 1   MARGKVQMKKIENPVHRQVTFCKRRAGLLKKAKELSVLCDAEIGVFIFSAHGKLYELATK 60
           M RGKV++K+IEN ++RQVTF KRR GLLKKA ELSVLCDAEI + IFS  GKLYE  + 
Sbjct: 1   MGRGKVELKRIENKINRQVTFAKRRNGLLKKAYELSVLCDAEIALLIFSNRGKLYEFCSS 60

Query: 61  GNALGG-----------------------------------------------GGAEGIM 113
            + +                                                 G     M
Sbjct: 61  PSGMARTVDKYRKHSYATMDPNQSAKDLQDKYQDYLKLKSRVEILQHSQRHLLGEELSEM 120

[Close]

BLAST of Lsi08G000400 vs. TAIR 10
Match: AT2G03710.2 (K-box region and MADS-box transcription factor family protein )

HSP 1 Score: 86.3 bits (212), Expect = 2.2e-17
Identity = 55/159 (34.59%), Postives = 78/159 (49.06%), Query Frame = 0


Query: 1   MARGKVQMKKIENPVHRQVTFCKRRAGLLKKAKELSVLCDAEIGVFIFSAHGKLYELATK 60
           M RGKV++K+IEN ++RQVTF KRR GLLKKA ELSVLCDAEI + IFS  GKLYE  + 
Sbjct: 1   MGRGKVELKRIENKINRQVTFAKRRNGLLKKAYELSVLCDAEIALLIFSNRGKLYEFCSS 60

Query: 61  GNALGG-----------------------------------------------GGAEGIM 113
            + +                                                 G     M
Sbjct: 61  PSGMARTVDKYRKHSYATMDPNQSAKDLQDKYQDYLKLKSRVEILQHSQRHLLGEELSEM 120

The following BLAST results are available for this feature:

BLAST of Lsi08G000400 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q38841	1.7e-33	43.81	Agamous-like MADS-box protein AGL12 OS=Arabidopsis thaliana OX=3702 GN=AGL12 PE=...	[more]
Q2QW55	3.8e-30	48.72	MADS-box transcription factor 33 OS=Oryza sativa subsp. japonica OX=39947 GN=MAD...	[more]
A2YQK9	7.2e-29	46.45	MADS-box transcription factor 26 OS=Oryza sativa subsp. indica OX=39946 GN=MADS2...	[more]
Q0J8G8	7.2e-29	46.45	MADS-box transcription factor 26 OS=Oryza sativa subsp. japonica OX=39947 GN=MAD...	[more]
Q8LLR2	3.0e-19	35.20	Agamous-like MADS-box protein MADS2 OS=Vitis vinifera OX=29760 GN=MADS2 PE=2 SV=...	[more]

BLAST of Lsi08G000400 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KW77	2.0e-61	66.67	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G650620 PE=4 SV=1	[more]
A0A6J1IA69	2.2e-60	65.53	agamous-like MADS-box protein AGL12 OS=Cucurbita maxima OX=3661 GN=LOC111472603 ...	[more]
A0A6J1ECC1	2.2e-60	65.53	agamous-like MADS-box protein AGL12 OS=Cucurbita moschata OX=3662 GN=LOC11143278...	[more]
A0A6J1D898	8.5e-57	67.71	agamous-like MADS-box protein AGL12 isoform X2 OS=Momordica charantia OX=3673 GN...	[more]
A0A1S4E2G4	3.0e-54	67.39	agamous-like MADS-box protein AGL12 OS=Cucumis melo OX=3656 GN=LOC103498765 PE=4...	[more]

BLAST of Lsi08G000400 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038891170.1	1.6e-62	67.80	agamous-like MADS-box protein AGL12 [Benincasa hispida]	[more]
XP_011656111.1	4.0e-61	66.67	agamous-like MADS-box protein AGL12 [Cucumis sativus] >KGN52697.1 hypothetical p...	[more]
XP_022973991.1	4.5e-60	65.53	agamous-like MADS-box protein AGL12 [Cucurbita maxima]	[more]
XP_022925494.1	4.5e-60	65.53	agamous-like MADS-box protein AGL12 [Cucurbita moschata] >XP_023536341.1 agamous...	[more]
KAG6592380.1	4.5e-60	65.53	Agamous-like MADS-box protein AGL12, partial [Cucurbita argyrosperma subsp. soro...	[more]

BLAST of Lsi08G000400 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G71692.1	1.2e-34	43.81	AGAMOUS-like 12	[more]
AT5G15800.1	5.9e-18	36.31	K-box region and MADS-box transcription factor family protein	[more]
AT5G15800.2	5.9e-18	36.31	K-box region and MADS-box transcription factor family protein	[more]
AT2G03710.1	2.2e-17	34.59	K-box region and MADS-box transcription factor family protein	[more]
AT2G03710.2	2.2e-17	34.59	K-box region and MADS-box transcription factor family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 97..121
None	No IPR available	PANTHER	PTHR48019	SERUM RESPONSE FACTOR HOMOLOG	coord: 1..61 coord: 67..141
None	No IPR available	PANTHER	PTHR48019:SF45	AGAMOUS-LIKE MADS-BOX PROTEIN AGL12	coord: 1..61
None	No IPR available	PANTHER	PTHR48019:SF45	AGAMOUS-LIKE MADS-BOX PROTEIN AGL12	coord: 67..141
IPR002100	Transcription factor, MADS-box	PRINTS	PR00404	MADSDOMAIN	coord: 23..38 score: 80.42 coord: 38..59 score: 50.51 coord: 3..23 score: 59.36
IPR002100	Transcription factor, MADS-box	SMART	SM00432	madsneu2	coord: 1..60 e-value: 4.6E-35 score: 132.5
IPR002100	Transcription factor, MADS-box	PFAM	PF00319	SRF-TF	coord: 10..56 e-value: 1.0E-22 score: 79.4
IPR002100	Transcription factor, MADS-box	PROSITE	PS50066	MADS_BOX_2	coord: 1..61 score: 29.295868
IPR002487	Transcription factor, K-box	PFAM	PF01486	K-box	coord: 73..113 e-value: 2.4E-8 score: 34.0
IPR036879	Transcription factor, MADS-box superfamily	GENE3D	3.40.1810.10		coord: 15..112 e-value: 3.0E-21 score: 77.0
IPR036879	Transcription factor, MADS-box superfamily	SUPERFAMILY	55455	SRF-like	coord: 1..60
IPR033896	MADS MEF2-like	CDD	cd00265	MADS_MEF2_like	coord: 2..62 e-value: 2.49118E-36 score: 118.039

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi08G000400.1	Lsi08G000400.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0045944	positive regulation of transcription by RNA polymerase II
biological_process	GO:0006355	regulation of transcription, DNA-templated
cellular_component	GO:0005634	nucleus
molecular_function	GO:0003700	DNA-binding transcription factor activity
molecular_function	GO:0046983	protein dimerization activity
molecular_function	GO:0000977	RNA polymerase II transcription regulatory region sequence-specific DNA binding
molecular_function	GO:0003677	DNA binding