Lsi08G000170 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi08G000170
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Descriptionprotein NRT1/ PTR FAMILY 5.10-like
Locationchr08: 398045 .. 404062 (+)
RNA-Seq ExpressionLsi08G000170
SyntenyLsi08G000170
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGATATGGCTGATGAATCCCGAAGGCCGCTGATATTAAACGACGACGTCGTACCGGGGTCCGTGGACCACCGAGAGCGTCCGAGCCGGCGATCAGAGTCTGGGCGGTGGAGGGCGGCGGGGTTGATAATTGGAGTTGAAATAGCGGAGCGGTTGGCATTCTTCGGGATAAGCACCAATCTGGTAAGCTACTTGACAGTGGAGATGGGGCAGTCCATGGCCGCCGCCGCCAAGAATGTAAATCTCTGGATCGGAACCGCCTCTTTGCTCCCATTGCTTGCCGCCTCTTTCGCCGATTCCTTCCTCGGACGCTACCTTACTATTCTTCTCGCCTCCGCTCTTTACATCTTGGTTAACCTTCTTTTTCCTTTTCTTTTTCTCATTCTCATTCTTTTAAACATCATCATAATAAACAAATTATGTAAAACACTTCATTTTTTCTTAAAATATATAAGTTCTGACTCTTTTTAATTTTCTAAATCTATATACTTAACTGAGGGGGCAATTTCTTTTATGACATAAACAAAAGGTGAAATAGATAACAGTTAGAGAGGGGGAGAGGAAAATATAGGAATAATAAAGAAGTCTAACGTCATAAATTAAATGGGGAGACAGAGGAAATGGAAGCGGTTGAATCTTTAGAATTAAGGTAATATTGCACTTTTGAATGTTTAGAGTTATTAACGGTTTAGGCATTTTAAGAATAATAATTGTGTATATGATAATATTTTTAAAAATTTGCAAATATAGTAAAATCTATTCGTGATTAGCTCTATTACTACTAGACTTCTACTAGTTATGATCTATCATTGATAGATTTTGAGAATTGGATCTAAATTTTGTCATATCTACAAATTCTTTTGGAAATTTTCACCAATAAAAAAAAGGAAACTATTTAAAAAAAATAGAAAAACAAAATAGCAAAAACATCACTAATAGACAGTTCTATCCGCATCTATCATTGATAGATTTCTATTATTTCTATCATTGATAGATTTCTATCATCATCTATCAAACTATCTAAAAATTTTGCTATTTTGTGTGGATGGTTTCCTTTATTTTTCTATTTTTAAAAACTCCCCAATTTTTTTTTTTTTTTTTACCATCTACATTGAGCCTCATCACTATATTTGCAACTAGTCTTAAATTTTAATGACTAACCATTTAGTATTTATACTTGTAACAATTTTTTTCTTTATTATAAAAAATCTTATGAAAACTAATGGTTTTCTTTTTTTTTTTTTTTTCCTTTGAGAAAAAATTACTTTATTAAGCCACATGGGCATTAGCATCAATTGCAGCAATGATTAACGGTTAATTTACCACGATTTAGTTTTATAGTTTATAAATAAATGTGATTTTCCATGGGTAATCTTGATAATATATATTTTCACAATACAAAGTTTAAATCATTACAAATGGTACAATGGTAAAGTACAAGCGCACTAAGTTCTTACTTATGGACTAAATTGTTTCCATTTGAAAGTATCCTTAACTAACTACTGTTAAGTTGAGAATTAAATTATAATTCTAAAAAAAAAAATTGAGAATTAAATTATTATTATTATTATTATTATTATTAGTTTTTTTTATAAAAAAAAGCCTAATAGGTTTAAAAAAATTAGAGGAAAAATTTCAAATTATTTACCAATATAGAAATTAGCTATAGATTTTTATTGTTATCTATCGCGATACAATTCTATCACCGATAGACCGTAATTTTTTTTAGATTTATAAATAGTTTGACTCATTTTTCTATATTTGAAAACGGTCTATGAGTTTAAGTTTTGTTTTTTTTAATGAATGAACTGAAATGCAGGGACTTGGCCTACTGACACTCTCAGCTACGCTCCGGGGATCTGGCTCTGCTGCCACGGGAGCTTCATCTCAGCCTGTTCCTGATGCTGATGTCGTGTTGTTCTTCAGCGCTTTGTACTTGGTGGCATTTGCACAAGGTGGACACAAGCCTTGTGTTCAAGCATTTGGTTGTGATCAATTTGATGGAGAAGATCCTCAAGAATGCATAGCCAAATGCTCTTTCTTCAACTGGTGGTACTTTGCCACTACTTTTGGATCCTTCATTGCTCTAATCATTTTAAACTACATTCAAGATAACCTCGGTTGGGGTCTTGGCTTCGGCATTCCCTGCATCTCCTCCGTAGCTGCTCTCGTTGTGTTCTTGCTCGGGACTCGGACTTATCGTTTCGTTACAGTGGCCAACAATGAGGAGAAAACATTCATGAGAATTGGTCGGGTTTTTGTCAATGCGGCTCGAAACTGGAGAACGACATCGTCGGACATTGCTATTTTGGAGGAAGGTCAAGATGCAATGCTTTATCAAAGATATGGACAATTGAGGTAAGCTAGTTGAGATTTCCCTTTCTTATTATTTTTCTTTCATCCTATATATTGATGTTGATTAAGCAATGTTTTTCCCCTTTAATTTATTTCATAAATCTTCTTTTGTGTTTACAATTAAATTGTTTGTTTTGTTTTTTTTCCGTAATTTATTTTTATAATTTTATTTCAATTCTAATAAAAGAGATGTGGTTATGCATATGGTGTCCTTGACCAATGGGAAGCAGCCAATTATGTAGAATATGGACAATTCTATATTTGTCTATTCAATGTCTCTTATTGTGTAGGAACTTCTAATGGATTTTAGGAAGCAACAATATATGATGGCTAATCTATTCGGAACACAAAATTTTTTTATTTAATATTAATATTATTATTATTATTTTGACAATAAGTGGGTTAGGGGAATCAAACCCGACTAGTACTTTGAGCTAGGCTCATTTTGGTAAATTTGGACTGATTTTGTTAATTTAAAACGTCGTATTTCATTATTCCTTTTGAAATTTGGACAACATTTCTAATGGATTTCTAAAGGGTTAAAATGTTGACTCAATTTCATAGAAATGTATTGATGAATATTTCTCCTAAAAGTTTAATTACAAAAAAAAAAAAAAAAATGAAGGAAAATTAATAAATAAACATTTTGTTTTTTTTAAATAAGTTAAAATATTGTTTATTTATATTTAATTCATATTAGTGACATATAAATTTGCTTATTTTGTATGTTTTCATTATTTTTTTTTTTCATTGAATAAGATATTGATTCATTCCACGTTGAATTTATGGGTATATGACAATGCCGATGAAAAATGTTTGACATGTTAACAAAAACATTAACACACTTTTTACTACATAGCAAAAGAAAAAATACTAGAGAAACTAGAGAACTCATGTATATGAAAGAAAATGAAGATTAAAATTAGGTTAAATAACAAGTTTAAGTTCGTAAGTTTGAGATTTGTTTTTATTTGATAGGTAGAAGAGGTGTTATAGGCTTCATTTAAAAAAAATTACTAATAAGTTTTTTTAATTGTGTCTAACATGTTCTTGAAAAAATTGAATACAAATTTTATTTTGGGAAAAGAGAAGACAAGACAATTTAACCCATAGCTTCTCATTTCCAGCCTTAGGAATAAGGGGTCGGGGGGATTCCCCATACCTAACTCTGTCTCCCAATTTGAGGGGAACAGGGGGTTCTGTCGGGGAATCACGTCGGTGATTTCCATCTCTGTTTTTATGGAAAATTTTTCGTTATTTCTCAATTTTTTTAAAATTTTTTTTATTAGCTATTATTTTTTAATCTATACTTTCGTTTAATAATACTTTTTAATTTTAGATTGGAGTATCTTTTTTCTGTGTAGATTACAAACCTTTGTGAAAAAAAATTAATAAACACAAATGCATACCTTTGAAAAAAATACATTAATAATAAAATGTGTTATTTTTAAATATAGAAAAATGAGTCAAACTATTTTTATATTTCACTATCTATCAGCGATAGAGTTCTATCACGATCTATCGTTTGAGCGATAGACAACAATAGAAGTCTATCAAGTGATAGATGTCTATCGCGGTCTATCGTTAATAGATAGTAATTTATTTTTTATTTTTCATAAAGTAAAATTAAGTAAGTGATATAATGTCATAGTTGTCTTCTTTCTAAGTAGAAAAATATATTAGTACAATAGAATGTAAAACTATAATAATATAATCAATAAGAAAACTATATCTGGTCATCTTCTTGTCCTTTAATCCCAATAACAAACTAAAACTAATAAAGAAAAATAGTTGGGGAGACCATTCTTCGTGAGTTTTTTTCCCTACCTTACTCTTCTTTCTTTGTGAGAATAAAGGAGGAAAATATGGCTGAAAATGATCCAAAACTCAAAAACTTCTAAAACAAATGTCCTCTTTTTAAAAGAACATGTTTTTTATTCTTTGTTGAGAAGTGAGATTATCATTTTATCCTTATAGTACAAAACATTAAATATCATAAATGATTTAAGTTCCGATGCCACTCAAAAGGCAAGATGTTTAAAATTTTTCTCAATTTATAAACATGTTTGGAAAGAACACGAAAACAATGTATGAAAAATGAGTGGTCATTTACCTAAAACGGATTTGTATCAATGAAGATATGATAGATCAAAACAAAATTACTTCGTGGATCGCGCAATAAAAAATGCGTCATTGCGCATACCTCTCGTTGTGCGCACTCTTATACTTCTCGCTTTGCGTTTTTTTTTGTCATAGCTCTCTTGATACAACTTCGTTTTAGCTAGAGGATCACTCCTTCTTATACATTTTTTCATGTTATTTTTAACCATGCTTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTAAAGAATCGTGAATTTAAATGGCCTTAAAAAGTTTCAACATAGAGTAATTTGGCTTTTGGGTATTGAAGCTAAAGATTTTTTTTTTAATTTATAAGCATGGTGGGAAAGAATACGAAAAAAAAACGTTAGAAAACATATACACGTGGTCATTTTTAGCAAAAGTCAAGTTGTATCAAAAGAGATATGAGAAAAGGTTTAAAAACATGTTTCTTTGTTTTTTTTTTTTTTTTTTTAAAGAAAATGAGGTCATTTGTAAAAAAGTTTTAAAAAGTTTTTGGGTTTTAGATTACTTTGAACGATTTTCCACCTCTTAGAAAAAGAAATACATCATATTATAAGATATGTGAGATCTTCAAAAGAGGATAGAGGTTGATATCGATAGTAAAAGTTTTATGTCATTTTCATACTACAGTTTTAATGTGGTGTATTTTGAATGTAACTGAGTGAGTGTTTTAAGTTGACAGGTTCCTTAACAAAGCCTTGGTTGCTCGAATGGACTCGAATAAAGATTTGAAAACGTGTAACATAGTGGAGGTTGAAGAAGCTAAGGGTATCCTAAGGCTGATCCCCATTTGGACAGCAAGCTTGGCATATGCCATTGTTTTATCACAATGCTCTACCTTCTTCGTCAAGCAAGCTTCCACCATGGACAGATCAATCTCACCAACCTTCAAAATACCCGCTGCTACAATCCAATGCTTTGCCCTCATTGCTGTTCTCCTCTTCCTTCCAATCTATGACCAACTCCTCGTTCCCATCGCCAGAATATTCACCCTCAAACCCTCCGGAATCTCCATGCTCCAAAGAATTGGAGTTGGGATGTTCATATCCACTCTTTCAATGGTTGTTGCCGCTCTTGTTGAATGTAAAAGGCTAGCCGTCGCTCGAGAACATGGCTTAACTCATAACCCTAACGCTACGATCCCTATCACCATCTGGTGGCTAACTCCACAGCTCATCCTCCTCGGAGTTTCTAGCGTTTTCACGATGGTAGGTTTGCAGGAGTTCTTCTACGACCAAGTTTTGATCGAGCTGAAGAGTGTGGGACTTGCACTGTATCTGAGCATCTTTGGAGTGGGGAACTTGCTTAGCGGTGTTCTTGTATCCATCATTGAAGACACAACTGGTCGACATGGAAGAGGTGGCTGGCTTGCTAACAACATAAATAATGCACATCTTGATTACTTTTATTGGCTTCTTGCTGGCATTGGTGAGGTAGGATTACTTGCATATATGTATTTTGCTAACTCTTATGTATATAAATACAACGTCGAAAGATCTAGAGATTAA

mRNA sequence

ATGGATATGGCTGATGAATCCCGAAGGCCGCTGATATTAAACGACGACGTCGTACCGGGGTCCGTGGACCACCGAGAGCGTCCGAGCCGGCGATCAGAGTCTGGGCGGTGGAGGGCGGCGGGGTTGATAATTGGAGTTGAAATAGCGGAGCGGTTGGCATTCTTCGGGATAAGCACCAATCTGGTAAGCTACTTGACAGTGGAGATGGGGCAGTCCATGGCCGCCGCCGCCAAGAATGTAAATCTCTGGATCGGAACCGCCTCTTTGCTCCCATTGCTTGCCGCCTCTTTCGCCGATTCCTTCCTCGGACGCTACCTTACTATTCTTCTCGCCTCCGCTCTTTACATCTTGGTTAACCTTCTTTTTCCTTTTCTTTTTCTCATTCTCATTCTTTTAAACATCATCATAATAAACAAATTATCTACGCTCCGGGGATCTGGCTCTGCTGCCACGGGAGCTTCATCTCAGCCTGTTCCTGATGCTGATGTCGTGTTGTTCTTCAGCGCTTTGTACTTGGTGGCATTTGCACAAGGTGGACACAAGCCTTGTGTTCAAGCATTTGGTTGTGATCAATTTGATGGAGAAGATCCTCAAGAATGCATAGCCAAATGCTCTTTCTTCAACTGGTGGTACTTTGCCACTACTTTTGGATCCTTCATTGCTCTAATCATTTTAAACTACATTCAAGATAACCTCGGTTGGGGTCTTGGCTTCGGCATTCCCTGCATCTCCTCCGTAGCTGCTCTCGTTGTGTTCTTGCTCGGGACTCGGACTTATCGTTTCGTTACAGTGGCCAACAATGAGGAGAAAACATTCATGAGAATTGGTCGGGTTTTTGTCAATGCGGCTCGAAACTGGAGAACGACATCGTCGGACATTGCTATTTTGGAGGAAGGTCAAGATGCAATGCTTTATCAAAGATATGGACAATTGAGGTTCCTTAACAAAGCCTTGGTTGCTCGAATGGACTCGAATAAAGATTTGAAAACGTGTAACATAGTGGAGGTTGAAGAAGCTAAGGGTATCCTAAGGCTGATCCCCATTTGGACAGCAAGCTTGGCATATGCCATTGTTTTATCACAATGCTCTACCTTCTTCGTCAAGCAAGCTTCCACCATGGACAGATCAATCTCACCAACCTTCAAAATACCCGCTGCTACAATCCAATGCTTTGCCCTCATTGCTGTTCTCCTCTTCCTTCCAATCTATGACCAACTCCTCGTTCCCATCGCCAGAATATTCACCCTCAAACCCTCCGGAATCTCCATGCTCCAAAGAATTGGAGTTGGGATGTTCATATCCACTCTTTCAATGGTTGTTGCCGCTCTTGTTGAATGTAAAAGGCTAGCCGTCGCTCGAGAACATGGCTTAACTCATAACCCTAACGCTACGATCCCTATCACCATCTGGTGGCTAACTCCACAGCTCATCCTCCTCGGAGTTTCTAGCGTTTTCACGATGGTAGGTTTGCAGGAGTTCTTCTACGACCAAGTTTTGATCGAGCTGAAGAGTGTGGGACTTGCACTGTATCTGAGCATCTTTGGAGTGGGGAACTTGCTTAGCGGTGTTCTTGTATCCATCATTGAAGACACAACTGGTCGACATGGAAGAGGTGGCTGGCTTGCTAACAACATAAATAATGCACATCTTGATTACTTTTATTGGCTTCTTGCTGGCATTGGTGAGGTAGGATTACTTGCATATATGTATTTTGCTAACTCTTATGTATATAAATACAACGTCGAAAGATCTAGAGATTAA

Coding sequence (CDS)

ATGGATATGGCTGATGAATCCCGAAGGCCGCTGATATTAAACGACGACGTCGTACCGGGGTCCGTGGACCACCGAGAGCGTCCGAGCCGGCGATCAGAGTCTGGGCGGTGGAGGGCGGCGGGGTTGATAATTGGAGTTGAAATAGCGGAGCGGTTGGCATTCTTCGGGATAAGCACCAATCTGGTAAGCTACTTGACAGTGGAGATGGGGCAGTCCATGGCCGCCGCCGCCAAGAATGTAAATCTCTGGATCGGAACCGCCTCTTTGCTCCCATTGCTTGCCGCCTCTTTCGCCGATTCCTTCCTCGGACGCTACCTTACTATTCTTCTCGCCTCCGCTCTTTACATCTTGGTTAACCTTCTTTTTCCTTTTCTTTTTCTCATTCTCATTCTTTTAAACATCATCATAATAAACAAATTATCTACGCTCCGGGGATCTGGCTCTGCTGCCACGGGAGCTTCATCTCAGCCTGTTCCTGATGCTGATGTCGTGTTGTTCTTCAGCGCTTTGTACTTGGTGGCATTTGCACAAGGTGGACACAAGCCTTGTGTTCAAGCATTTGGTTGTGATCAATTTGATGGAGAAGATCCTCAAGAATGCATAGCCAAATGCTCTTTCTTCAACTGGTGGTACTTTGCCACTACTTTTGGATCCTTCATTGCTCTAATCATTTTAAACTACATTCAAGATAACCTCGGTTGGGGTCTTGGCTTCGGCATTCCCTGCATCTCCTCCGTAGCTGCTCTCGTTGTGTTCTTGCTCGGGACTCGGACTTATCGTTTCGTTACAGTGGCCAACAATGAGGAGAAAACATTCATGAGAATTGGTCGGGTTTTTGTCAATGCGGCTCGAAACTGGAGAACGACATCGTCGGACATTGCTATTTTGGAGGAAGGTCAAGATGCAATGCTTTATCAAAGATATGGACAATTGAGGTTCCTTAACAAAGCCTTGGTTGCTCGAATGGACTCGAATAAAGATTTGAAAACGTGTAACATAGTGGAGGTTGAAGAAGCTAAGGGTATCCTAAGGCTGATCCCCATTTGGACAGCAAGCTTGGCATATGCCATTGTTTTATCACAATGCTCTACCTTCTTCGTCAAGCAAGCTTCCACCATGGACAGATCAATCTCACCAACCTTCAAAATACCCGCTGCTACAATCCAATGCTTTGCCCTCATTGCTGTTCTCCTCTTCCTTCCAATCTATGACCAACTCCTCGTTCCCATCGCCAGAATATTCACCCTCAAACCCTCCGGAATCTCCATGCTCCAAAGAATTGGAGTTGGGATGTTCATATCCACTCTTTCAATGGTTGTTGCCGCTCTTGTTGAATGTAAAAGGCTAGCCGTCGCTCGAGAACATGGCTTAACTCATAACCCTAACGCTACGATCCCTATCACCATCTGGTGGCTAACTCCACAGCTCATCCTCCTCGGAGTTTCTAGCGTTTTCACGATGGTAGGTTTGCAGGAGTTCTTCTACGACCAAGTTTTGATCGAGCTGAAGAGTGTGGGACTTGCACTGTATCTGAGCATCTTTGGAGTGGGGAACTTGCTTAGCGGTGTTCTTGTATCCATCATTGAAGACACAACTGGTCGACATGGAAGAGGTGGCTGGCTTGCTAACAACATAAATAATGCACATCTTGATTACTTTTATTGGCTTCTTGCTGGCATTGGTGAGGTAGGATTACTTGCATATATGTATTTTGCTAACTCTTATGTATATAAATACAACGTCGAAAGATCTAGAGATTAA

Protein sequence

MDMADESRRPLILNDDVVPGSVDHRERPSRRSESGRWRAAGLIIGVEIAERLAFFGISTNLVSYLTVEMGQSMAAAAKNVNLWIGTASLLPLLAASFADSFLGRYLTILLASALYILVNLLFPFLFLILILLNIIIINKLSTLRGSGSAATGASSQPVPDADVVLFFSALYLVAFAQGGHKPCVQAFGCDQFDGEDPQECIAKCSFFNWWYFATTFGSFIALIILNYIQDNLGWGLGFGIPCISSVAALVVFLLGTRTYRFVTVANNEEKTFMRIGRVFVNAARNWRTTSSDIAILEEGQDAMLYQRYGQLRFLNKALVARMDSNKDLKTCNIVEVEEAKGILRLIPIWTASLAYAIVLSQCSTFFVKQASTMDRSISPTFKIPAATIQCFALIAVLLFLPIYDQLLVPIARIFTLKPSGISMLQRIGVGMFISTLSMVVAALVECKRLAVAREHGLTHNPNATIPITIWWLTPQLILLGVSSVFTMVGLQEFFYDQVLIELKSVGLALYLSIFGVGNLLSGVLVSIIEDTTGRHGRGGWLANNINNAHLDYFYWLLAGIGEVGLLAYMYFANSYVYKYNVERSRD
Homology
BLAST of Lsi08G000170 vs. ExPASy Swiss-Prot
Match: Q0WP01 (Protein NRT1/ PTR FAMILY 5.10 OS=Arabidopsis thaliana OX=3702 GN=NPF5.10 PE=2 SV=1)

HSP 1 Score: 605.9 bits (1561), Expect = 4.8e-172
Identity = 320/574 (55.75%), Postives = 405/574 (70.56%), Query Frame = 0

Query: 5   DESRRPLILNDDVVPGSVDHRERPSRRSESGRWRAAGLIIGVEIAERLAFFGISTNLVSY 64
           DE+  PL+        +VD+R +P+ +S SG WR+AG IIGVE+AER A++GIS+NL++Y
Sbjct: 8   DEAGTPLL------AVTVDYRNKPAVKSSSGGWRSAGFIIGVEVAERFAYYGISSNLITY 67

Query: 65  LTVEMGQSMAAAAKNVNLWIGTASLLPLLAASFADSFLGRYLTILLASALYILVNLLFPF 124
           LT  +GQS AAAA NVN W GTASLLPLL A  ADSFLGR+ TIL ASALYI        
Sbjct: 68  LTGPLGQSTAAAAANVNAWSGTASLLPLLGAFVADSFLGRFRTILAASALYI-------- 127

Query: 125 LFLILILLNIIIINKLSTLRGSGSAATGASSQPVPDADVVLFFSALYLVAFAQGGHKPCV 184
                + L ++    LS +  S    +   S   P   V+ FFSALYLVA AQGGHKPCV
Sbjct: 128 -----VGLGVL---TLSAMIPSDCKVSNLLSSCSPRFQVITFFSALYLVALAQGGHKPCV 187

Query: 185 QAFGCDQFDGEDPQECIAKCSFFNWWYFATTFGSFIALIILNYIQDNLGWGLGFGIPCIS 244
           QAFG DQFD ++P+EC AK SFFNWWYF   FG+   L +LNYIQDNL W LGFGIPCI+
Sbjct: 188 QAFGADQFDEKEPEECKAKSSFFNWWYFGMCFGTLTTLWVLNYIQDNLSWALGFGIPCIA 247

Query: 245 SVAALVVFLLGTRTYRFVTVANNEEKTFMRIGRVFVNAARNWRTTSSDIAILEEGQDAML 304
            V ALVV LLGT TYRF ++   ++  F+RIG V+V A +NW  ++ D+A  EE    + 
Sbjct: 248 MVVALVVLLLGTCTYRF-SIRREDQSPFVRIGNVYVAAVKNWSVSALDVAAAEERLGLVS 307

Query: 305 YQRYGQLRFLNKALVARMDSNKDLKTCNIVEVEEAKGILRLIPIWTASLAYAIVLSQCST 364
                Q  FLNKALVA+  S      C+I E+EEAK +LRL PIW   L YA+V +Q  T
Sbjct: 308 CSSSQQFSFLNKALVAKNGS------CSIDELEEAKSVLRLAPIWLTCLVYAVVFAQSPT 367

Query: 365 FFVKQASTMDRSISPTFKIPAATIQCFALIAVLLFLPIYDQLLVPIARIFTLKPSGISML 424
           FF KQ +TM+RSI+P +KI  AT+Q F  +++++F+PIYD++L+PIAR FT KP GI+ML
Sbjct: 368 FFTKQGATMERSITPGYKISPATLQSFISLSIVIFIPIYDRVLIPIARSFTHKPGGITML 427

Query: 425 QRIGVGMFISTLSMVVAALVECKRLAVAREHGLTHNPNATIPITIWWLTPQLILLGVSSV 484
           QRIG G+F+S L+MVVAALVE KRL  A ++GL  +P+AT+P+++WWL PQ +L G++ V
Sbjct: 428 QRIGTGIFLSFLAMVVAALVEMKRLKTAADYGLVDSPDATVPMSVWWLVPQYVLFGITDV 487

Query: 485 FTMVGLQEFFYDQVLIELKSVGLALYLSIFGVGNLLSGVLVSIIEDTTGRHGRGGWLANN 544
           F MVGLQEFFYDQV  EL+SVGLALYLSIFG+GN LS  ++SIIE  T + G+  W ANN
Sbjct: 488 FAMVGLQEFFYDQVPNELRSVGLALYLSIFGIGNFLSSFMISIIEKATSQSGQASWFANN 547

Query: 545 INNAHLDYFYWLLAGIGEVGLLAYMYFANSYVYK 579
           +N AHLDYFYWLLA +  +GL +Y+Y A SYV K
Sbjct: 548 LNQAHLDYFYWLLACLSFIGLASYLYVAKSYVSK 552

BLAST of Lsi08G000170 vs. ExPASy Swiss-Prot
Match: Q9C7U1 (Protein NRT1/ PTR FAMILY 5.12 OS=Arabidopsis thaliana OX=3702 GN=NPF5.12 PE=2 SV=1)

HSP 1 Score: 531.9 bits (1369), Expect = 8.8e-150
Identity = 288/571 (50.44%), Postives = 380/571 (66.55%), Query Frame = 0

Query: 12  ILNDDVVPGSVDHRERPSRRSESGRWRAAGLIIGVEIAERLAFFGISTNLVSYLTVEMGQ 71
           I++++ V  SVD R  PS RS SG W+++G  +  E+AE+ A+FGI++NL++Y T  +G+
Sbjct: 13  IVSNENVEFSVDFRGNPSIRSSSGAWKSSGFTMCAEVAEKFAYFGIASNLITYFTEALGE 72

Query: 72  SMAAAAKNVNLWIGTASLLPLLAASFADSFLGRYLTILLASALYILVNLLFPFLFLILIL 131
           S A AA NVNLW+GTA+ LPL+  S ADSFLGR+ TILL S+ YI+   L  F   I  L
Sbjct: 73  STAVAASNVNLWLGTAAFLPLIWGSIADSFLGRFRTILLTSSFYIMGLGLLTFSATIPSL 132

Query: 132 LNIIIINKLSTLRGSGSAATGASSQPVPDADVVLFFSALYLVAFAQGGHKPCVQAFGCDQ 191
            N                 T  S   V    V++FF ALYL+A  +GG K C++AFG DQ
Sbjct: 133 CN--------------DQETRESC--VSQVKVIIFFCALYLIALGEGGFKVCLRAFGADQ 192

Query: 192 FDGEDPQECIAKCSFFNWWYFATTFGSFIALIILNYIQDNLGWGLGFGIPCISSVAALVV 251
           FD +DP E  AK S+FNW YFA + G     ++ NY+Q+NL W LG+ IPC+S + AL +
Sbjct: 193 FDEQDPNESKAKSSYFNWLYFAISIGILTTRLVTNYVQENLSWALGYAIPCLSMMLALFL 252

Query: 252 FLLGTRTYRFVT-----VANNEEKTFMRIGRVFVNAARNWRTTSSDIAILEEGQDAMLYQ 311
           FLLG +TYRF T          +  F+RIGRVFV AARN R T SD  +L   +      
Sbjct: 253 FLLGIKTYRFSTGGEGRQGKKHDNPFVRIGRVFVAAARNRRQTPSDTCLLLPNESTK--- 312

Query: 312 RYGQLRFLNKALVARMDSNKDLKTCNIVEVEEAKGILRLIPIWTASLAYAIVLSQCSTFF 371
              + RFL++A++          +C+  EVEEAK +L LIPIW  SL + IV +Q  TFF
Sbjct: 313 ---KFRFLDRAVI----------SCDSYEVEEAKAVLSLIPIWLCSLVFGIVFAQSPTFF 372

Query: 372 VKQASTMDRSISPTFKIPAATIQCFALIAVLLFLPIYDQLLVPIARIFTLKPSGISMLQR 431
            KQ STMDRSIS T ++PAAT+QCF  +A+L+F+PIYD+L VPIAR  T KP+GI+ LQR
Sbjct: 373 TKQGSTMDRSISSTLQVPAATLQCFISLAILVFIPIYDRLFVPIARSITRKPAGITTLQR 432

Query: 432 IGVGMFISTLSMVVAALVECKRLAVAREHGLTHNPNATIPITIWWLTPQLILLGVSSVFT 491
           I  G+F+S +SMV+AALVE KRL  AR+HGL  +P AT+P+++ WL PQ IL GVS VFT
Sbjct: 433 ISTGIFLSIISMVIAALVEMKRLKTARDHGLVDSPKATVPMSVCWLIPQYILFGVSDVFT 492

Query: 492 MVGLQEFFYDQVLIELKSVGLALYLSIFGVGNLLSGVLVSIIEDTTGRHGRGGWLANNIN 551
           MVGLQEFFY +V  +L+S+GLALYLSI G+GN LS  +VS+IE+ T + G+  W +NN+N
Sbjct: 493 MVGLQEFFYGEVPPQLRSMGLALYLSIIGIGNFLSSFMVSVIEEATSQSGQVSWFSNNLN 551

Query: 552 NAHLDYFYWLLAGIGEVGLLAYMYFANSYVY 578
            AHLDYFYWLLA +  +  +  +YFA SY+Y
Sbjct: 553 QAHLDYFYWLLACLSSLAFIFTVYFAKSYLY 551

BLAST of Lsi08G000170 vs. ExPASy Swiss-Prot
Match: Q0WSZ6 (Protein NRT1/ PTR FAMILY 5.13 OS=Arabidopsis thaliana OX=3702 GN=NPF5.13 PE=2 SV=2)

HSP 1 Score: 514.6 bits (1324), Expect = 1.5e-144
Identity = 277/567 (48.85%), Postives = 379/567 (66.84%), Query Frame = 0

Query: 13  LNDDVVPGSVDHRERPSRRSESGRWRAAGLIIGVEIAERLAFFGISTNLVSYLTVEMGQS 72
           L ++ V  +VDHR   +RRS +GRWRAA  IIGVE+AER A +GI +NL+SYLT  +GQS
Sbjct: 9   LQEEYVIDAVDHRGFSARRSITGRWRAAWFIIGVEVAERFANYGIGSNLISYLTGPLGQS 68

Query: 73  MAAAAKNVNLWIGTASLLPLLAASFADSFLGRYLTILLASALYILVNLLFPFLFLILILL 132
            A AA NVN W G +++LPLL A  AD+FLGRY+TI++AS +Y+        L L  + L
Sbjct: 69  TAVAAANVNAWSGISTILPLLGAFVADAFLGRYITIIIASFIYV--------LGLAFLTL 128

Query: 133 NIIIINKLSTLRGSGSAATGASSQPVPDADVVLFFSALYLVAFAQGGHKPCVQAFGCDQF 192
           +  +I   + +  S S+   A           LFF +LYLVA  Q GHKPCVQAFG DQF
Sbjct: 129 SAFLIPNNTEVTSSPSSFLNA-----------LFFFSLYLVAIGQSGHKPCVQAFGADQF 188

Query: 193 DGEDPQECIAKCSFFNWWYFATTFGSFIALIILNYIQDNLGWGLGFGIPCISSVAALVVF 252
           D ++PQE   + SFFNWWY +   G  +A++++ YIQ+N+ W LGFGIPC+  V +LV+F
Sbjct: 189 DEKNPQENSDRSSFFNWWYLSMCAGIGLAILVVVYIQENVSWALGFGIPCVFMVISLVLF 248

Query: 253 LLGTRTYRFVTVANNEE-KTFMRIGRVFVNAARNWRTTSSDIAILEEGQDAMLYQRYGQL 312
           +LG ++YRF      EE   F RIGRVF  A +N R  SSD+  +E  +     +   +L
Sbjct: 249 VLGRKSYRFSKTRQEEETNPFTRIGRVFFVAFKNQRLNSSDLCKVELIEANRSQESPEEL 308

Query: 313 RFLNKALVARMDSNKDLKTCNIVEVEEAKGILRLIPIWTASLAYAIVLSQCSTFFVKQAS 372
            FLNKAL+   DS++    C   +VE+A  ++RLIP+W  +LAYAI  +Q  TFF KQ  
Sbjct: 309 SFLNKALLVPNDSDEGEVACKSRDVEDATALVRLIPVWLTTLAYAIPFAQYMTFFTKQGV 368

Query: 373 TMDRSISPTFKIPAATIQCFALIAVLLFLPIYDQLLVPIARIFTLKPSGISMLQRIGVGM 432
           TM+R+I P  +IP A++Q    I+++LF+PIYD++LVPI R  T  P GI+ L+RIG GM
Sbjct: 369 TMERTIFPGVEIPPASLQVLISISIVLFVPIYDRVLVPIGRSITKDPCGITTLKRIGTGM 428

Query: 433 FISTLSMVVAALVECKRLAVAREHGLTHNPNATIPITIWWLTPQLILLGVSSVFTMVGLQ 492
            ++TL+MVVAALVE KRL  A+E+GL   P  T+P++IWWL PQ +LLG++ V T+VG+Q
Sbjct: 429 VLATLTMVVAALVESKRLETAKEYGLIDQPKTTLPMSIWWLFPQYMLLGLADVHTLVGMQ 488

Query: 493 EFFYDQVLIELKSVGLALYLSIFGVGNLLSGVLVSIIEDTTGRHGRGGWLANNINNAHLD 552
           EFFY QV  EL+S+GLA+YLS  GVG+LLS +L+ +I+  TG      W  +N+N AHLD
Sbjct: 489 EFFYSQVPTELRSLGLAIYLSAMGVGSLLSSLLIYLIDLATGGDAGNSWFNSNLNRAHLD 548

Query: 553 YFYWLLAGIGEVGLLAYMYFANSYVYK 579
           YFYWLLA +  VG   +++ + SY+Y+
Sbjct: 549 YFYWLLAVVSAVGFFTFLFISKSYIYR 556

BLAST of Lsi08G000170 vs. ExPASy Swiss-Prot
Match: Q8VZE2 (Protein NRT1/ PTR FAMILY 5.14 OS=Arabidopsis thaliana OX=3702 GN=NPF5.14 PE=2 SV=2)

HSP 1 Score: 513.5 bits (1321), Expect = 3.2e-144
Identity = 275/567 (48.50%), Postives = 378/567 (66.67%), Query Frame = 0

Query: 13  LNDDVVPGSVDHRERPSRRSESGRWRAAGLIIGVEIAERLAFFGISTNLVSYLTVEMGQS 72
           L ++ V  +VDHR   +RRS +GRWRAA  IIGVE+AER A++GI +NL+SYLT  +G+S
Sbjct: 9   LQEEYVTDAVDHRGLAARRSNTGRWRAALFIIGVEVAERFAYYGIGSNLISYLTGPLGES 68

Query: 73  MAAAAKNVNLWIGTASLLPLLAASFADSFLGRYLTILLASALYILVNLLFPFLFLILILL 132
            A AA NVN W G A+LLP+L A  AD+FLGRY TI+++S +Y+        L L  + L
Sbjct: 69  TAVAAANVNAWSGIATLLPVLGAFVADAFLGRYRTIIISSLIYV--------LGLAFLTL 128

Query: 133 NIIIINKLSTLRGSGSAATGASSQPVPDADVVLFFSALYLVAFAQGGHKPCVQAFGCDQF 192
           +  +I   + +  S S+              VLFF +LYLVA  Q GHKPCVQAFG DQF
Sbjct: 129 SAFLIPNTTEVTSSTSSFLN-----------VLFFFSLYLVAIGQSGHKPCVQAFGADQF 188

Query: 193 DGEDPQECIAKCSFFNWWYFATTFGSFIALIILNYIQDNLGWGLGFGIPCISSVAALVVF 252
           D +D QE   + SFFNWWY + + G   A++++ YIQ+   W  GFGIPC+  V +LV+F
Sbjct: 189 DEKDSQEKSDRSSFFNWWYLSLSAGICFAILVVVYIQEEFSWAFGFGIPCVFMVISLVLF 248

Query: 253 LLGTRTYRFVTVANNEE-KTFMRIGRVFVNAARNWRTTSSDIAILEEGQDAMLYQRYGQL 312
           + G R YR+    + EE   F RIGRVF  A +N R +SSD+  +E   +    ++    
Sbjct: 249 VSGRRIYRYSKRRHEEEINPFTRIGRVFFVALKNQRLSSSDLCKVELEANTSPEKQ---- 308

Query: 313 RFLNKALVARMDSNKDLKTCNIVEVEEAKGILRLIPIWTASLAYAIVLSQCSTFFVKQAS 372
            F NKAL+   DS++        +VE+A  ++RLIP+W  +LAYAI  +Q  TFF KQ  
Sbjct: 309 SFFNKALLVPNDSSQGENASKSSDVEDATALIRLIPVWFTTLAYAIPYAQYMTFFTKQGV 368

Query: 373 TMDRSISPTFKIPAATIQCFALIAVLLFLPIYDQLLVPIARIFTLKPSGISMLQRIGVGM 432
           TMDR+I P  KIP A++Q F  I+++LF+PIYD++ VPIAR+ T +P GI+ L+RIG G+
Sbjct: 369 TMDRTILPGVKIPPASLQVFIGISIVLFVPIYDRVFVPIARLITKEPCGITTLKRIGTGI 428

Query: 433 FISTLSMVVAALVECKRLAVAREHGLTHNPNATIPITIWWLTPQLILLGVSSVFTMVGLQ 492
            +ST++MV+AALVE KRL  A+EHGL   P AT+P++IWWL PQ +LLG++ V+T+VG+Q
Sbjct: 429 VLSTITMVIAALVEFKRLETAKEHGLIDQPEATLPMSIWWLIPQYLLLGLADVYTLVGMQ 488

Query: 493 EFFYDQVLIELKSVGLALYLSIFGVGNLLSGVLVSIIEDTTGRHGRGGWLANNINNAHLD 552
           EFFY QV  EL+S+GLALYLS  GVG+LLS +L+S+I+  TG      W  +N+N AHLD
Sbjct: 489 EFFYSQVPTELRSIGLALYLSALGVGSLLSSLLISLIDLATGGDAGNSWFNSNLNRAHLD 548

Query: 553 YFYWLLAGIGEVGLLAYMYFANSYVYK 579
           YFYWLLA +  VG   +++ + SY+Y+
Sbjct: 549 YFYWLLAIVSAVGFFTFLFISKSYIYR 552

BLAST of Lsi08G000170 vs. ExPASy Swiss-Prot
Match: Q9SK96 (Protein NRT1/ PTR FAMILY 5.16 OS=Arabidopsis thaliana OX=3702 GN=NPF5.16 PE=2 SV=1)

HSP 1 Score: 502.3 bits (1292), Expect = 7.5e-141
Identity = 276/568 (48.59%), Postives = 380/568 (66.90%), Query Frame = 0

Query: 13  LNDDVVPGSVDHRERPSRRSESGRWRAAGLIIGVEIAERLAFFGISTNLVSYLTVEMGQS 72
           L +D V  SVDHR  P+ +S +G WR+A  IIGVE+ ER A+FGI +NL++YLT  +GQS
Sbjct: 10  LIEDSVSDSVDHRGLPAGKSSTGGWRSAWYIIGVEVGERFAYFGIGSNLITYLTGPLGQS 69

Query: 73  MAAAAKNVNLWIGTASLLPLLAASFADSFLGRYLTILLASALYILVNLLFPFLFLILILL 132
            A AA NVN W GTAS+LP+L A  AD++LGRY TI++AS +YIL          +L L 
Sbjct: 70  TATAAVNVNTWSGTASILPVLGAFIADAYLGRYRTIVVASLIYILG-------LGLLTLS 129

Query: 133 NIIIINKLSTLRGSGSAATGASSQPVPDADVVLFFSALYLVAFAQGGHKPCVQAFGCDQF 192
           +I+I+  LS  R        AS++P    + +LFF +LYLVA  QGGHKPCVQAFG DQF
Sbjct: 130 SILILMGLSEQRQHN---RNASAKPFFWVN-ILFFCSLYLVAIGQGGHKPCVQAFGADQF 189

Query: 193 DGEDPQECIAKCSFFNWWYFATTFGSFIALIILNYIQDNLGWGLGFGIPCISSVAALVVF 252
           D  DP+E I++ SFFNWW+ + + G  +++I++ Y+QDN+ W LGFGIPC+  V AL +F
Sbjct: 190 DVGDPKERISRGSFFNWWFLSLSAGITLSIIVVVYVQDNVNWALGFGIPCLFMVMALALF 249

Query: 253 LLGTRTYRFVT-VANNEEKTFMRIGRVFVNAARNWRTTSSDIAILEEGQDAMLYQR-YGQ 312
           L G +TYR+       +   F RIGRVF+ A +N +   +    LE G     Y++  GQ
Sbjct: 250 LFGRKTYRYPRGDREGKNNAFARIGRVFLVAFKNRKLKLTHSGQLEVGS----YKKCKGQ 309

Query: 313 LRFLNKALVARMDSNKDLKTCNIVEVEEAKGILRLIPIWTASLAYAIVLSQCSTFFVKQA 372
           L FL KAL   +     ++ C+  +VE+A  ++RLIPIW  S+   I  +Q +TFF KQ 
Sbjct: 310 LEFLAKAL---LPGEGGVEPCSSRDVEDAMALVRLIPIWITSVVSTIPYAQYATFFTKQG 369

Query: 373 STMDRSISPTFKIPAATIQCFALIAVLLFLPIYDQLLVPIARIFTLKPSGISMLQRIGVG 432
            T+DR I P F+IP A+ Q    +++ + +P Y+++ +P+AR+ T KPSGI+MLQRIG G
Sbjct: 370 VTVDRKILPGFEIPPASFQALIGLSIFISVPTYERVFLPLARLITKKPSGITMLQRIGAG 429

Query: 433 MFISTLSMVVAALVECKRLAVAREHGLTHNPNATIPITIWWLTPQLILLGVSSVFTMVGL 492
           M +S+L+MVVAALVE KRL  A+EHGL   P+ATIP++IWW  PQ +LLG+  VF++VG 
Sbjct: 430 MVLSSLNMVVAALVEMKRLETAKEHGLVDRPDATIPMSIWWFVPQYLLLGMIDVFSLVGT 489

Query: 493 QEFFYDQVLIELKSVGLALYLSIFGVGNLLSGVLVSIIEDTTGRHGRGGWLANNINNAHL 552
           QEFFYDQV  EL+S+GLAL LS  G+ + LSG L+++I   TG++G   W   N+N AH+
Sbjct: 490 QEFFYDQVPTELRSIGLALSLSAMGLASFLSGFLITVINWATGKNGGDSWFNTNLNRAHV 549

Query: 553 DYFYWLLAGIGEVGLLAYMYFANSYVYK 579
           DYFYWLLA    +G LA++  +  YVY+
Sbjct: 550 DYFYWLLAAFTAIGFLAFLLLSRLYVYR 559

BLAST of Lsi08G000170 vs. ExPASy TrEMBL
Match: A0A5D3BMJ3 (Protein NRT1/ PTR FAMILY 5.10-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold169G00060 PE=3 SV=1)

HSP 1 Score: 876.3 bits (2263), Expect = 7.0e-251
Identity = 470/576 (81.60%), Postives = 511/576 (88.72%), Query Frame = 0

Query: 12  ILNDDVVPGSVDHRERPSRRSESGRWRAAGLIIGVEIAERLAFFGISTNLVSYLTVEMGQ 71
           ILN D +PG        +RRS+SG WRAAG+IIGVEIAER AFFGISTNLVSYLTVEM Q
Sbjct: 45  ILN-DAIPG--------NRRSQSGGWRAAGVIIGVEIAERFAFFGISTNLVSYLTVEMEQ 104

Query: 72  SMAAAAKNVNLWIGTASLLPLLAASFADSFLGRYLTILLASALYILVNLLFPFLFLILIL 131
           SMA+AA+NVNLWIGTASLLPL+AASFADSFLGRYLTI+LASALYI        L L L+ 
Sbjct: 105 SMASAAQNVNLWIGTASLLPLVAASFADSFLGRYLTIILASALYI--------LGLGLLT 164

Query: 132 LNIIIINKLSTLRGSG---SAATGASSQPVPDADVVLFFSALYLVAFAQGGHKPCVQAFG 191
           L+ ++ +  S+ +GSG   SAATGASS+PV    VVLFFS+LYLVAFAQGGHKPC+QAFG
Sbjct: 165 LSAMLASP-SSFQGSGSAASAATGASSRPV--LHVVLFFSSLYLVAFAQGGHKPCLQAFG 224

Query: 192 CDQFDGEDPQECIAKCSFFNWWYFATTFGSFIALIILNYIQDNLGWGLGFGIPCISSVAA 251
           CDQFDGEDPQECIAKCSFFNWWYFATT GSFIALIIL+YIQDNLGW LGFGIPCISS+ A
Sbjct: 225 CDQFDGEDPQECIAKCSFFNWWYFATTLGSFIALIILSYIQDNLGWCLGFGIPCISSLVA 284

Query: 252 LVVFLLGTRTYRFVTVANNEEKTFMRIGRVFVNAARNWRTTSSDIAILEEGQDAMLYQRY 311
           L+VFLLGT+TYRFVT+AN EEK FMRIGRVF NAARNWRT SS+I I EEGQDAMLY+R 
Sbjct: 285 LLVFLLGTQTYRFVTIANYEEKPFMRIGRVFFNAARNWRTASSEIDISEEGQDAMLYRRC 344

Query: 312 GQLRFLNKALVARMDSNKDLKTCNIVEVEEAKGILRLIPIWTASLAYAIVLSQCSTFFVK 371
           GQLRFLNKALVA +DS+ D KTCNIVEVEEAKG+LRLIPIW ASL+YAIVLSQCSTFFVK
Sbjct: 345 GQLRFLNKALVAPVDSDDDGKTCNIVEVEEAKGVLRLIPIWIASLSYAIVLSQCSTFFVK 404

Query: 372 QASTMDRSISPTFKIPAATIQCFALIAVLLFLPIYDQLLVPIARIFTLKPSGISMLQRIG 431
           Q STMDRSI P+FKIPAATIQCF  IAV+LF+PIYD+LLVPIARIFTLKPSGISMLQRIG
Sbjct: 405 QGSTMDRSIRPSFKIPAATIQCFGCIAVVLFVPIYDRLLVPIARIFTLKPSGISMLQRIG 464

Query: 432 VGMFISTLSMVVAALVECKRLAVAREHGLTHNPNATIPITIWWLTPQLILLGVSSVFTMV 491
           VGMFISTLSMVVAALVE KRLAVAREHGLT NPN+TIPITIWWLTPQL+LLGVSSVFTMV
Sbjct: 465 VGMFISTLSMVVAALVEVKRLAVAREHGLTQNPNSTIPITIWWLTPQLLLLGVSSVFTMV 524

Query: 492 GLQEFFYDQVLIELKSVGLALYLSIFGVGNLLSGVLVSIIEDTT-GRHGRGGWLANNINN 551
           GLQEFFYDQV  E+KSVGLALYLSIFGVGNLLS +LVS+IE+ T G  GRGGW ANNIN 
Sbjct: 525 GLQEFFYDQVSSEVKSVGLALYLSIFGVGNLLSSLLVSVIEEATGGDDGRGGWFANNINK 584

Query: 552 AHLDYFYWLLAGIGEVGLLAYMYFANSYVYKYNVER 584
           AHLDYFYWLLAGIG+VGLLAY+YFANSYVYKYNVER
Sbjct: 585 AHLDYFYWLLAGIGKVGLLAYVYFANSYVYKYNVER 600

BLAST of Lsi08G000170 vs. ExPASy TrEMBL
Match: A0A1S4E2H8 (protein NRT1/ PTR FAMILY 5.10-like OS=Cucumis melo OX=3656 GN=LOC107991676 PE=3 SV=1)

HSP 1 Score: 876.3 bits (2263), Expect = 7.0e-251
Identity = 469/576 (81.42%), Postives = 512/576 (88.89%), Query Frame = 0

Query: 12  ILNDDVVPGSVDHRERPSRRSESGRWRAAGLIIGVEIAERLAFFGISTNLVSYLTVEMGQ 71
           ILN D +PG        +RRS+SG WRAAG+IIGVEIAER AFFGISTNLVSYLTVEM Q
Sbjct: 45  ILN-DAIPG--------NRRSQSGGWRAAGVIIGVEIAERFAFFGISTNLVSYLTVEMEQ 104

Query: 72  SMAAAAKNVNLWIGTASLLPLLAASFADSFLGRYLTILLASALYILVNLLFPFLFLILIL 131
           SMA+AA+NVNLWIGTASLLPL+AASFADSFLGRYLTI+LASALYI        L L L+ 
Sbjct: 105 SMASAAQNVNLWIGTASLLPLVAASFADSFLGRYLTIILASALYI--------LGLGLLT 164

Query: 132 LNIIIINKLSTLRGSG---SAATGASSQPVPDADVVLFFSALYLVAFAQGGHKPCVQAFG 191
           L+ ++ +  S+ +GSG   SAATGAS++PV    VVLFFS+LYLVAFAQGGHKPC+QAFG
Sbjct: 165 LSAMLASP-SSFQGSGSAASAATGASARPV--LHVVLFFSSLYLVAFAQGGHKPCLQAFG 224

Query: 192 CDQFDGEDPQECIAKCSFFNWWYFATTFGSFIALIILNYIQDNLGWGLGFGIPCISSVAA 251
           CDQFDGEDPQECIAKCSFFNWWYFATT GSFIALIIL+YIQDNLGW LGFGIPCISS+ A
Sbjct: 225 CDQFDGEDPQECIAKCSFFNWWYFATTLGSFIALIILSYIQDNLGWCLGFGIPCISSLVA 284

Query: 252 LVVFLLGTRTYRFVTVANNEEKTFMRIGRVFVNAARNWRTTSSDIAILEEGQDAMLYQRY 311
           L+VFLLGT+TYRFVT+AN+EEK FMRIGRVF NAARNWRT SS+I I EEGQDAMLY+R 
Sbjct: 285 LLVFLLGTQTYRFVTIANDEEKPFMRIGRVFFNAARNWRTASSEIDISEEGQDAMLYRRC 344

Query: 312 GQLRFLNKALVARMDSNKDLKTCNIVEVEEAKGILRLIPIWTASLAYAIVLSQCSTFFVK 371
           GQLRFLNKALVA +DS+ D KTCNIVEVEEAKG+LRLIPIW ASL+YAIVLSQCSTFFVK
Sbjct: 345 GQLRFLNKALVAPVDSDDDGKTCNIVEVEEAKGVLRLIPIWIASLSYAIVLSQCSTFFVK 404

Query: 372 QASTMDRSISPTFKIPAATIQCFALIAVLLFLPIYDQLLVPIARIFTLKPSGISMLQRIG 431
           Q STMDRSI P+FKIPAATIQCF  IAV+LF+PIYD+LLVPIARIFTLKPSGISMLQRIG
Sbjct: 405 QGSTMDRSIRPSFKIPAATIQCFGCIAVVLFVPIYDRLLVPIARIFTLKPSGISMLQRIG 464

Query: 432 VGMFISTLSMVVAALVECKRLAVAREHGLTHNPNATIPITIWWLTPQLILLGVSSVFTMV 491
           VGMFISTLSMVVAALVE KRLAVAREHGLT NPN+TIPITIWWLTPQL+LLGVSSVFTMV
Sbjct: 465 VGMFISTLSMVVAALVEVKRLAVAREHGLTQNPNSTIPITIWWLTPQLLLLGVSSVFTMV 524

Query: 492 GLQEFFYDQVLIELKSVGLALYLSIFGVGNLLSGVLVSIIEDTT-GRHGRGGWLANNINN 551
           GLQEFFYDQV  E+KSVGLALYLSIFGVGNLLS +LVS+IE+ T G  GRGGW ANNIN 
Sbjct: 525 GLQEFFYDQVSSEVKSVGLALYLSIFGVGNLLSSLLVSVIEEATGGDDGRGGWFANNINK 584

Query: 552 AHLDYFYWLLAGIGEVGLLAYMYFANSYVYKYNVER 584
           AHLDYFYWLLAGIG+VGLLAY+YFANSYVYKYNVER
Sbjct: 585 AHLDYFYWLLAGIGKVGLLAYVYFANSYVYKYNVER 600

BLAST of Lsi08G000170 vs. ExPASy TrEMBL
Match: A0A6J1DMG4 (uncharacterized protein LOC111022389 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111022389 PE=3 SV=1)

HSP 1 Score: 738.8 bits (1906), Expect = 1.8e-209
Identity = 395/558 (70.79%), Postives = 460/558 (82.44%), Query Frame = 0

Query: 36   RWRAAGLIIGVEIAERLAFFGISTNLVSYLTVEMGQSMAAAAKNVNLWIGTASLLPLLAA 95
            RW +A  II VEIAE  AF+GI+TNL++YLT EMGQSMAAAA+NVN+W GTASLLPLL A
Sbjct: 579  RWTSAAFIIAVEIAELFAFYGINTNLITYLTGEMGQSMAAAARNVNIWTGTASLLPLLGA 638

Query: 96   SFADSFLGRYLTILLASALYILVNLLFPFLFLILILLNIIIINKLSTLRGSG---SAATG 155
            S ADS+LGRYLTILLASALY+        L L L+ L+ ++ +      GSG    + T 
Sbjct: 639  SLADSYLGRYLTILLASALYV--------LGLSLLTLSAVVAS------GSGFNDESGTR 698

Query: 156  ASSQPVPDADVVLFFSALYLVAFAQGGHKPCVQAFGCDQFDGEDPQ-ECIAKCSFFNWWY 215
            ASS+ + D   V+FF +LYLVAFAQGGHKPCVQAFGCDQFDGEDP  EC AKCSFFNWWY
Sbjct: 699  ASSRTLMD---VMFFISLYLVAFAQGGHKPCVQAFGCDQFDGEDPDLECRAKCSFFNWWY 758

Query: 216  FATTFGSFIALIILNYIQDNLGWGLGFGIPCISSVAALVVFLLGTRTYRFV--TVANNEE 275
            FATT GSF AL IL+YIQDNLGWGL FGIPCISS+ AL++FLLGT TYR V  TV ++ +
Sbjct: 759  FATTLGSFTALFILSYIQDNLGWGLAFGIPCISSLLALLIFLLGTPTYRCVTITVPDHGD 818

Query: 276  KTFMRIGRVFVNAARNWRTTSS-DIAILEE--GQDAMLYQRYGQLRFLNKALVARMDSNK 335
            K F+RIGRVFVNAARNWRTT + +I +LEE   QDAMLYQ+ G   FLNKALVA + S+K
Sbjct: 819  KPFVRIGRVFVNAARNWRTTPNLNIVVLEELGDQDAMLYQKSG--HFLNKALVAPVGSDK 878

Query: 336  DLKTCNIVEVEEAKGILRLIPIWTASLAYAIVLSQCSTFFVKQASTMDRSISPTFKIPAA 395
               TC++ EVEEAK +LRLIPIW ASL YA+VLSQCSTFFVKQ  TMDRSI+P+FKIPAA
Sbjct: 879  GGTTCSVSEVEEAKAVLRLIPIWVASLTYAVVLSQCSTFFVKQGYTMDRSITPSFKIPAA 938

Query: 396  TIQCFALIAVLLFLPIYDQLLVPIARIFTLKPSGISMLQRIGVGMFISTLSMVVAALVEC 455
            +IQCFA + ++LF+PIYD+++VPIAR FTLKPSGI+MLQRIGVGMFIST+SMVV ALVE 
Sbjct: 939  SIQCFACLGIVLFVPIYDRVVVPIARTFTLKPSGITMLQRIGVGMFISTISMVVGALVEA 998

Query: 456  KRLAVAREHGLTHNPNATIPITIWWLTPQLILLGVSSVFTMVGLQEFFYDQVLIELKSVG 515
            KRL++AREHGLT NPNATIPI++WWL PQ  LLGVS+VFTMVGLQEFFYDQV  ELKS+G
Sbjct: 999  KRLSIAREHGLTDNPNATIPISVWWLIPQFTLLGVSTVFTMVGLQEFFYDQVPSELKSLG 1058

Query: 516  LALYLSIFGVGNLLSGVLVSIIEDTTGRHGRGGWLANNINNAHLDYFYWLLAGIGEVGLL 575
            LAL LSIFGVG+LLSGVLV ++ED T  +GR GWL NN+N AHLDYFYWLLAG+GEVGL+
Sbjct: 1059 LALNLSIFGVGSLLSGVLVCVVEDATVGYGRDGWLDNNMNRAHLDYFYWLLAGLGEVGLV 1117

Query: 576  AYMYFANSYVYKYNVERS 585
            AY+YFANSY+YKY+  RS
Sbjct: 1119 AYLYFANSYLYKYSAGRS 1117

BLAST of Lsi08G000170 vs. ExPASy TrEMBL
Match: A0A5B6YPP0 (Uncharacterized protein OS=Davidia involucrata OX=16924 GN=Din_003158 PE=3 SV=1)

HSP 1 Score: 662.1 bits (1707), Expect = 2.1e-186
Identity = 335/568 (58.98%), Postives = 421/568 (74.12%), Query Frame = 0

Query: 12  ILNDDVVPGSVDHRERPSRRSESGRWRAAGLIIGVEIAERLAFFGISTNLVSYLTVEMGQ 71
           +L+DDVV G+VD++ RP  RS+SG WR+A  IIGVEIAER A++GIS+NL+SYLT  +GQ
Sbjct: 14  LLDDDVVDGAVDYKGRPVTRSKSGGWRSASFIIGVEIAERFAYYGISSNLISYLTGPLGQ 73

Query: 72  SMAAAAKNVNLWIGTASLLPLLAASFADSFLGRYLTILLASALYILVNLLFPFLFLILIL 131
           S A AA NVN W G ASLLPLL A  AD+FLGRY TI++AS LYIL              
Sbjct: 74  STAMAAANVNAWSGAASLLPLLGAFVADTFLGRYRTIIIASLLYILG------------- 133

Query: 132 LNIIIINKLSTLRGSGSAATGASSQPVP--DADVVLFFSALYLVAFAQGGHKPCVQAFGC 191
           L ++ I+ +    GS        + P    +  V+ FF ALYLVAFAQGGHKPCVQAFG 
Sbjct: 134 LGLLTISAVLPSLGSSDYQNYVKNMPSSPHELQVIFFFFALYLVAFAQGGHKPCVQAFGA 193

Query: 192 DQFDGEDPQECIAKCSFFNWWYFATTFGSFIALIILNYIQDNLGWGLGFGIPCISSVAAL 251
           DQFD +DP+EC AK SFFNWWYF+   G+   L+ILNYIQDNL WGLGFGIPC++   AL
Sbjct: 194 DQFDSQDPKECKAKSSFFNWWYFSLCAGTLTTLLILNYIQDNLNWGLGFGIPCVAMAIAL 253

Query: 252 VVFLLGTRTYRFVTVANNEEKTFMRIGRVFVNAARNWRTTSSDIAILEEGQDAMLYQRYG 311
           V+FLLG+ TYRF ++  +E+  F+RIG+VF+ AA+NWRT SS IAI EE Q  + +Q   
Sbjct: 254 VIFLLGSMTYRF-SIKGDEKSPFLRIGQVFIKAAKNWRTPSSAIAIEEEAQGILPHQGSQ 313

Query: 312 QLRFLNKALVARMDSNKDLKTCNIVEVEEAKGILRLIPIWTASLAYAIVLSQCSTFFVKQ 371
           Q +FLNKAL+A   S +D K C+I EVEEAK +LRL+PIW   L YA+V +Q STFF KQ
Sbjct: 314 QFKFLNKALLAPNGSKEDGKVCSINEVEEAKAVLRLVPIWATCLVYAVVFAQSSTFFTKQ 373

Query: 372 ASTMDRSISPTFKIPAATIQCFALIAVLLFLPIYDQLLVPIARIFTLKPSGISMLQRIGV 431
             TMDRSI P+F +PAAT+Q F  ++++LF+PIYD++LVPIAR  T KPSGI+MLQRIG 
Sbjct: 374 GVTMDRSIGPSFDVPAATLQSFISLSIVLFIPIYDRILVPIARAITRKPSGITMLQRIGT 433

Query: 432 GMFISTLSMVVAALVECKRLAVAREHGLTHNPNATIPITIWWLTPQLILLGVSSVFTMVG 491
           GMF+S +SM +AA+ E KRL  A E+GL   PNAT+P+++WWL PQ +L G++ VFTMVG
Sbjct: 434 GMFLSAISMAIAAMTERKRLETALEYGLVDKPNATVPMSVWWLVPQYLLFGIADVFTMVG 493

Query: 492 LQEFFYDQVLIELKSVGLALYLSIFGVGNLLSGVLVSIIEDTTGRHGRGGWLANNINNAH 551
           LQEFFYDQV  ELKSVGL+LYLSIFG+G+ LS  L+S+I+  T   G+ GW ++N+N AH
Sbjct: 494 LQEFFYDQVPTELKSVGLSLYLSIFGIGSFLSSFLISVIQKATSADGQDGWFSDNLNRAH 553

Query: 552 LDYFYWLLAGIGEVGLLAYMYFANSYVY 578
           LDYFYWLLAG+  VGL+AY+YFA SY+Y
Sbjct: 554 LDYFYWLLAGLSSVGLIAYLYFAKSYIY 567

BLAST of Lsi08G000170 vs. ExPASy TrEMBL
Match: A0A5B6YQ37 (Uncharacterized protein OS=Davidia involucrata OX=16924 GN=Din_003159 PE=3 SV=1)

HSP 1 Score: 660.6 bits (1703), Expect = 6.1e-186
Identity = 334/568 (58.80%), Postives = 420/568 (73.94%), Query Frame = 0

Query: 12  ILNDDVVPGSVDHRERPSRRSESGRWRAAGLIIGVEIAERLAFFGISTNLVSYLTVEMGQ 71
           +L+DDVV G+VD++ RP  RS+SG WR+A  IIGVEIAER A++GIS+NL+SYLT  +GQ
Sbjct: 14  LLDDDVVDGAVDYKGRPVTRSKSGGWRSASFIIGVEIAERFAYYGISSNLISYLTGPLGQ 73

Query: 72  SMAAAAKNVNLWIGTASLLPLLAASFADSFLGRYLTILLASALYILVNLLFPFLFLILIL 131
           S A AA NVN W G ASLLPLL A  AD+FLGRY TI++AS LYIL              
Sbjct: 74  STAMAAANVNAWSGAASLLPLLGAFVADTFLGRYRTIIIASLLYILG------------- 133

Query: 132 LNIIIINKLSTLRGSGSAATGASSQPVP--DADVVLFFSALYLVAFAQGGHKPCVQAFGC 191
           L ++ I+ +    GS        + P    +  V+ FF ALYLVAFAQGGHKPCVQAFG 
Sbjct: 134 LGLLTISAVLPSLGSSDYQNYVKNMPSSPHELQVIFFFFALYLVAFAQGGHKPCVQAFGA 193

Query: 192 DQFDGEDPQECIAKCSFFNWWYFATTFGSFIALIILNYIQDNLGWGLGFGIPCISSVAAL 251
           DQFD +DP+EC AK SFFNWWYF+   G+   L+ILNYIQDNL WGLGFGIPC++   AL
Sbjct: 194 DQFDSQDPKECKAKSSFFNWWYFSLCAGTLTTLLILNYIQDNLNWGLGFGIPCVAMAIAL 253

Query: 252 VVFLLGTRTYRFVTVANNEEKTFMRIGRVFVNAARNWRTTSSDIAILEEGQDAMLYQRYG 311
           V+FLLG+ TYRF ++  +E+  F+RIG+VF+ A +NWRT SS IAI EE Q  + +Q   
Sbjct: 254 VIFLLGSMTYRF-SIKGDEKSPFLRIGQVFIKAGKNWRTPSSAIAIEEEAQGILPHQGSQ 313

Query: 312 QLRFLNKALVARMDSNKDLKTCNIVEVEEAKGILRLIPIWTASLAYAIVLSQCSTFFVKQ 371
           Q +FLNKAL+A   S +D K C+I EVEEAK +LRL+PIW   L YA+V +Q STFF KQ
Sbjct: 314 QFKFLNKALLAPNGSKEDGKVCSINEVEEAKAVLRLVPIWATCLVYAVVFAQSSTFFTKQ 373

Query: 372 ASTMDRSISPTFKIPAATIQCFALIAVLLFLPIYDQLLVPIARIFTLKPSGISMLQRIGV 431
             TMDRSI P+F +PAAT+Q F  ++++LF+PIYD++LVPIAR  T KPSGI+MLQRIG 
Sbjct: 374 GVTMDRSIGPSFDVPAATLQSFISLSIVLFIPIYDRILVPIARAITRKPSGITMLQRIGT 433

Query: 432 GMFISTLSMVVAALVECKRLAVAREHGLTHNPNATIPITIWWLTPQLILLGVSSVFTMVG 491
           GMF+S +SM +AA+ E KRL  A E+GL   PNAT+P+++WWL PQ +L G++ VFTMVG
Sbjct: 434 GMFLSAISMAIAAMTERKRLETALEYGLVDKPNATVPMSVWWLVPQYLLFGIADVFTMVG 493

Query: 492 LQEFFYDQVLIELKSVGLALYLSIFGVGNLLSGVLVSIIEDTTGRHGRGGWLANNINNAH 551
           LQEFFYDQV  ELKSVGL+LYLSIFG+G+ LS  L+S+I+  T   G+ GW ++N+N AH
Sbjct: 494 LQEFFYDQVPTELKSVGLSLYLSIFGIGSFLSSFLISVIQKATSADGQDGWFSDNLNRAH 553

Query: 552 LDYFYWLLAGIGEVGLLAYMYFANSYVY 578
           LDYFYWLLAG+  VGL+AY+YFA SY+Y
Sbjct: 554 LDYFYWLLAGLSSVGLIAYLYFAKSYIY 567

BLAST of Lsi08G000170 vs. NCBI nr
Match: XP_038891003.1 (protein NRT1/ PTR FAMILY 5.10-like [Benincasa hispida])

HSP 1 Score: 937.9 bits (2423), Expect = 4.1e-269
Identity = 494/584 (84.59%), Postives = 530/584 (90.75%), Query Frame = 0

Query: 1   MDMADESRRPLILNDDVVPGSVDHRERPSRRSESGRWRAAGLIIGVEIAERLAFFGISTN 60
           MD  D+S RPLILN + V GSVDHR RPS+RSESG WRAAG+IIGVEIAERLAFFGISTN
Sbjct: 47  MDDMDDSGRPLILN-EAVQGSVDHRGRPSQRSESGGWRAAGMIIGVEIAERLAFFGISTN 106

Query: 61  LVSYLTVEMGQSMAAAAKNVNLWIGTASLLPLLAASFADSFLGRYLTILLASALYILVNL 120
           LV+YLTVEMGQSMA AA+NVNLW+GTASLLPLLAASFADSFLGRYLTILLASALYI    
Sbjct: 107 LVTYLTVEMGQSMATAAQNVNLWMGTASLLPLLAASFADSFLGRYLTILLASALYI---- 166

Query: 121 LFPFLFLILILLNIIIINKLSTLRGSGSAATGASSQPVPDADVVLFFSALYLVAFAQGGH 180
               L L L+ L+ ++ +  ++ +GS SAATG SS+PV   +VVLFFSALYLVAFAQGGH
Sbjct: 167 ----LGLALLTLSAMLASP-NSFQGSASAATGGSSRPV--LEVVLFFSALYLVAFAQGGH 226

Query: 181 KPCVQAFGCDQFDGEDPQECIAKCSFFNWWYFATTFGSFIALIILNYIQDNLGWGLGFGI 240
           KPCVQAFGCDQFDGE PQE IAKCSFFNWWYFATT GSFIALIIL+YIQDNLGWGLGFGI
Sbjct: 227 KPCVQAFGCDQFDGEHPQEYIAKCSFFNWWYFATTLGSFIALIILSYIQDNLGWGLGFGI 286

Query: 241 PCISSVAALVVFLLGTRTYRFVTVANNEEKTFMRIGRVFVNAARNWRTTSSDIAILEEGQ 300
           PCISSVAALVVFLLGTRTYRF+TVANN+EK FMRIGRVFVNAARNWRTTSSD+ ILEEGQ
Sbjct: 287 PCISSVAALVVFLLGTRTYRFLTVANNKEKPFMRIGRVFVNAARNWRTTSSDVVILEEGQ 346

Query: 301 DAMLYQRYGQLRFLNKALVARMDSNKDLKTCNIVEVEEAKGILRLIPIWTASLAYAIVLS 360
           DAMLYQR GQLRFLNKALVA MDS+KD KTCNI EVEEAKGILRLIPIW ASLAYAIVLS
Sbjct: 347 DAMLYQRAGQLRFLNKALVAPMDSDKDGKTCNIAEVEEAKGILRLIPIWIASLAYAIVLS 406

Query: 361 QCSTFFVKQASTMDRSISPTFKIPAATIQCFALIAVLLFLPIYDQLLVPIARIFTLKPSG 420
           QCSTFFVKQ STMDRSI+P F+IPAATIQCFA IAV+LF+PIYD+LLVP ARI TLKPSG
Sbjct: 407 QCSTFFVKQGSTMDRSITPNFQIPAATIQCFACIAVVLFVPIYDRLLVPTARILTLKPSG 466

Query: 421 ISMLQRIGVGMFISTLSMVVAALVECKRLAVAREHGLTHNPNATIPITIWWLTPQLILLG 480
           ISMLQRIGVGMFISTLSMVVAALVE KRL+VA+EHGLTH+PNATIPI IWWLTPQL+LLG
Sbjct: 467 ISMLQRIGVGMFISTLSMVVAALVELKRLSVAQEHGLTHDPNATIPIAIWWLTPQLLLLG 526

Query: 481 VSSVFTMVGLQEFFYDQVLIELKSVGLALYLSIFGVGNLLSGVLVSIIEDTTGRHGRGGW 540
           VSSVFTMVGLQEFFYDQV  EL+SVGLALYLSIFGVGNLLSGVLVS IED TG HGR GW
Sbjct: 527 VSSVFTMVGLQEFFYDQVSTELRSVGLALYLSIFGVGNLLSGVLVSAIEDATGGHGRSGW 586

Query: 541 LANNINNAHLDYFYWLLAGIGEVGLLAYMYFANSYVYKYNVERS 585
            ANNIN+AHLDYFYWLLAGIGEVGLLA+MYFANSY+YKYN+ERS
Sbjct: 587 FANNINSAHLDYFYWLLAGIGEVGLLAFMYFANSYLYKYNIERS 618

BLAST of Lsi08G000170 vs. NCBI nr
Match: KAE8649018.1 (hypothetical protein Csa_009254 [Cucumis sativus])

HSP 1 Score: 883.2 bits (2281), Expect = 1.2e-252
Identity = 464/557 (83.30%), Postives = 504/557 (90.48%), Query Frame = 0

Query: 29  SRRSESGRWRAAGLIIGVEIAERLAFFGISTNLVSYLTVEMGQSMAAAAKNVNLWIGTAS 88
           +RRS+SG WRAAG+IIGVEIAER AFFGISTNLVSYLTVEMGQSMA AA+NVNLW+GTAS
Sbjct: 53  NRRSQSGGWRAAGVIIGVEIAERFAFFGISTNLVSYLTVEMGQSMADAAQNVNLWVGTAS 112

Query: 89  LLPLLAASFADSFLGRYLTILLASALYILVNLLFPFLFLILILLNIIIINKLSTLRGSGS 148
           LLPLLAASFADSFLGRYLTI+LASALYI        L L L+ L+ I+ +  S+ +GSGS
Sbjct: 113 LLPLLAASFADSFLGRYLTIILASALYI--------LGLGLLTLSAILASP-SSFQGSGS 172

Query: 149 AATGASSQPVPDADVVLFFSALYLVAFAQGGHKPCVQAFGCDQFDGEDPQECIAKCSFFN 208
           AA+GASS+PV    V+ FF++LYLVAFAQGGHKPC+QAFGCDQFDGEDPQECIAKCSFFN
Sbjct: 173 AASGASSRPV--LHVLFFFTSLYLVAFAQGGHKPCLQAFGCDQFDGEDPQECIAKCSFFN 232

Query: 209 WWYFATTFGSFIALIILNYIQDNLGWGLGFGIPCISSVAALVVFLLGTRTYRFVTVANNE 268
           WWYF+TT GSFIALIIL+YIQDNLGWGLGFGIPCISS+ AL+VFLLGT TYRF  +AN+E
Sbjct: 233 WWYFSTTLGSFIALIILSYIQDNLGWGLGFGIPCISSLVALLVFLLGTHTYRFGKIANDE 292

Query: 269 EKTFMRIGRVFVNAARNWRTTSSDIAILEEGQDAMLYQRYGQLRFLNKALVARMDSNKDL 328
           EK FMRIGRVF NAARNWRT SS+I ILEEGQDAMLYQR GQLRFLNKALVA MDS++D 
Sbjct: 293 EKPFMRIGRVFFNAARNWRTKSSEIDILEEGQDAMLYQRSGQLRFLNKALVAPMDSDEDG 352

Query: 329 KTCNIVEVEEAKGILRLIPIWTASLAYAIVLSQCSTFFVKQASTMDRSISPTFKIPAATI 388
            TCNIVEVEEAKGILRLIPIW ASL+YAIVLSQCSTFFVKQ +TMDRSI+P+FKIPAATI
Sbjct: 353 NTCNIVEVEEAKGILRLIPIWIASLSYAIVLSQCSTFFVKQGATMDRSITPSFKIPAATI 412

Query: 389 QCFALIAVLLFLPIYDQLLVPIARIFTLKPSGISMLQRIGVGMFISTLSMVVAALVECKR 448
           QCF  IAV+ F+PIYD+LLVPIARIFTLKPSGISMLQRIGVGMFISTLSMVVAALVE KR
Sbjct: 413 QCFGCIAVVFFVPIYDRLLVPIARIFTLKPSGISMLQRIGVGMFISTLSMVVAALVEVKR 472

Query: 449 LAVAREHGLTHNPNATIPITIWWLTPQLILLGVSSVFTMVGLQEFFYDQVLIELKSVGLA 508
           LAVAR HGLT NPN+TIPITIWWLTPQL+LLGVSSVFTMVGLQEFFYDQV  E+KSVGLA
Sbjct: 473 LAVARAHGLTQNPNSTIPITIWWLTPQLLLLGVSSVFTMVGLQEFFYDQVSSEVKSVGLA 532

Query: 509 LYLSIFGVGNLLSGVLVSIIEDTT-GRHGRGGWLANNINNAHLDYFYWLLAGIGEVGLLA 568
           LYLSIFGVGNLLSG+LVS+IED T G  GRGGW ANNIN AHLDYFYWLLAGIG+VGLLA
Sbjct: 533 LYLSIFGVGNLLSGILVSVIEDATGGDDGRGGWFANNINTAHLDYFYWLLAGIGKVGLLA 592

Query: 569 YMYFANSYVYKYNVERS 585
           YMYFANSYVYKYNVERS
Sbjct: 593 YMYFANSYVYKYNVERS 598

BLAST of Lsi08G000170 vs. NCBI nr
Match: XP_011656124.2 (uncharacterized protein LOC105435629 [Cucumis sativus])

HSP 1 Score: 883.2 bits (2281), Expect = 1.2e-252
Identity = 464/557 (83.30%), Postives = 504/557 (90.48%), Query Frame = 0

Query: 29   SRRSESGRWRAAGLIIGVEIAERLAFFGISTNLVSYLTVEMGQSMAAAAKNVNLWIGTAS 88
            +RRS+SG WRAAG+IIGVEIAER AFFGISTNLVSYLTVEMGQSMA AA+NVNLW+GTAS
Sbjct: 622  NRRSQSGGWRAAGVIIGVEIAERFAFFGISTNLVSYLTVEMGQSMADAAQNVNLWVGTAS 681

Query: 89   LLPLLAASFADSFLGRYLTILLASALYILVNLLFPFLFLILILLNIIIINKLSTLRGSGS 148
            LLPLLAASFADSFLGRYLTI+LASALYI        L L L+ L+ I+ +  S+ +GSGS
Sbjct: 682  LLPLLAASFADSFLGRYLTIILASALYI--------LGLGLLTLSAILASP-SSFQGSGS 741

Query: 149  AATGASSQPVPDADVVLFFSALYLVAFAQGGHKPCVQAFGCDQFDGEDPQECIAKCSFFN 208
            AA+GASS+PV    V+ FF++LYLVAFAQGGHKPC+QAFGCDQFDGEDPQECIAKCSFFN
Sbjct: 742  AASGASSRPV--LHVLFFFTSLYLVAFAQGGHKPCLQAFGCDQFDGEDPQECIAKCSFFN 801

Query: 209  WWYFATTFGSFIALIILNYIQDNLGWGLGFGIPCISSVAALVVFLLGTRTYRFVTVANNE 268
            WWYF+TT GSFIALIIL+YIQDNLGWGLGFGIPCISS+ AL+VFLLGT TYRF  +AN+E
Sbjct: 802  WWYFSTTLGSFIALIILSYIQDNLGWGLGFGIPCISSLVALLVFLLGTHTYRFGKIANDE 861

Query: 269  EKTFMRIGRVFVNAARNWRTTSSDIAILEEGQDAMLYQRYGQLRFLNKALVARMDSNKDL 328
            EK FMRIGRVF NAARNWRT SS+I ILEEGQDAMLYQR GQLRFLNKALVA MDS++D 
Sbjct: 862  EKPFMRIGRVFFNAARNWRTKSSEIDILEEGQDAMLYQRSGQLRFLNKALVAPMDSDEDG 921

Query: 329  KTCNIVEVEEAKGILRLIPIWTASLAYAIVLSQCSTFFVKQASTMDRSISPTFKIPAATI 388
             TCNIVEVEEAKGILRLIPIW ASL+YAIVLSQCSTFFVKQ +TMDRSI+P+FKIPAATI
Sbjct: 922  NTCNIVEVEEAKGILRLIPIWIASLSYAIVLSQCSTFFVKQGATMDRSITPSFKIPAATI 981

Query: 389  QCFALIAVLLFLPIYDQLLVPIARIFTLKPSGISMLQRIGVGMFISTLSMVVAALVECKR 448
            QCF  IAV+ F+PIYD+LLVPIARIFTLKPSGISMLQRIGVGMFISTLSMVVAALVE KR
Sbjct: 982  QCFGCIAVVFFVPIYDRLLVPIARIFTLKPSGISMLQRIGVGMFISTLSMVVAALVEVKR 1041

Query: 449  LAVAREHGLTHNPNATIPITIWWLTPQLILLGVSSVFTMVGLQEFFYDQVLIELKSVGLA 508
            LAVAR HGLT NPN+TIPITIWWLTPQL+LLGVSSVFTMVGLQEFFYDQV  E+KSVGLA
Sbjct: 1042 LAVARAHGLTQNPNSTIPITIWWLTPQLLLLGVSSVFTMVGLQEFFYDQVSSEVKSVGLA 1101

Query: 509  LYLSIFGVGNLLSGVLVSIIEDTT-GRHGRGGWLANNINNAHLDYFYWLLAGIGEVGLLA 568
            LYLSIFGVGNLLSG+LVS+IED T G  GRGGW ANNIN AHLDYFYWLLAGIG+VGLLA
Sbjct: 1102 LYLSIFGVGNLLSGILVSVIEDATGGDDGRGGWFANNINTAHLDYFYWLLAGIGKVGLLA 1161

Query: 569  YMYFANSYVYKYNVERS 585
            YMYFANSYVYKYNVERS
Sbjct: 1162 YMYFANSYVYKYNVERS 1167

BLAST of Lsi08G000170 vs. NCBI nr
Match: XP_016902428.1 (PREDICTED: protein NRT1/ PTR FAMILY 5.10-like [Cucumis melo])

HSP 1 Score: 876.3 bits (2263), Expect = 1.5e-250
Identity = 469/576 (81.42%), Postives = 512/576 (88.89%), Query Frame = 0

Query: 12  ILNDDVVPGSVDHRERPSRRSESGRWRAAGLIIGVEIAERLAFFGISTNLVSYLTVEMGQ 71
           ILN D +PG        +RRS+SG WRAAG+IIGVEIAER AFFGISTNLVSYLTVEM Q
Sbjct: 45  ILN-DAIPG--------NRRSQSGGWRAAGVIIGVEIAERFAFFGISTNLVSYLTVEMEQ 104

Query: 72  SMAAAAKNVNLWIGTASLLPLLAASFADSFLGRYLTILLASALYILVNLLFPFLFLILIL 131
           SMA+AA+NVNLWIGTASLLPL+AASFADSFLGRYLTI+LASALYI        L L L+ 
Sbjct: 105 SMASAAQNVNLWIGTASLLPLVAASFADSFLGRYLTIILASALYI--------LGLGLLT 164

Query: 132 LNIIIINKLSTLRGSG---SAATGASSQPVPDADVVLFFSALYLVAFAQGGHKPCVQAFG 191
           L+ ++ +  S+ +GSG   SAATGAS++PV    VVLFFS+LYLVAFAQGGHKPC+QAFG
Sbjct: 165 LSAMLASP-SSFQGSGSAASAATGASARPV--LHVVLFFSSLYLVAFAQGGHKPCLQAFG 224

Query: 192 CDQFDGEDPQECIAKCSFFNWWYFATTFGSFIALIILNYIQDNLGWGLGFGIPCISSVAA 251
           CDQFDGEDPQECIAKCSFFNWWYFATT GSFIALIIL+YIQDNLGW LGFGIPCISS+ A
Sbjct: 225 CDQFDGEDPQECIAKCSFFNWWYFATTLGSFIALIILSYIQDNLGWCLGFGIPCISSLVA 284

Query: 252 LVVFLLGTRTYRFVTVANNEEKTFMRIGRVFVNAARNWRTTSSDIAILEEGQDAMLYQRY 311
           L+VFLLGT+TYRFVT+AN+EEK FMRIGRVF NAARNWRT SS+I I EEGQDAMLY+R 
Sbjct: 285 LLVFLLGTQTYRFVTIANDEEKPFMRIGRVFFNAARNWRTASSEIDISEEGQDAMLYRRC 344

Query: 312 GQLRFLNKALVARMDSNKDLKTCNIVEVEEAKGILRLIPIWTASLAYAIVLSQCSTFFVK 371
           GQLRFLNKALVA +DS+ D KTCNIVEVEEAKG+LRLIPIW ASL+YAIVLSQCSTFFVK
Sbjct: 345 GQLRFLNKALVAPVDSDDDGKTCNIVEVEEAKGVLRLIPIWIASLSYAIVLSQCSTFFVK 404

Query: 372 QASTMDRSISPTFKIPAATIQCFALIAVLLFLPIYDQLLVPIARIFTLKPSGISMLQRIG 431
           Q STMDRSI P+FKIPAATIQCF  IAV+LF+PIYD+LLVPIARIFTLKPSGISMLQRIG
Sbjct: 405 QGSTMDRSIRPSFKIPAATIQCFGCIAVVLFVPIYDRLLVPIARIFTLKPSGISMLQRIG 464

Query: 432 VGMFISTLSMVVAALVECKRLAVAREHGLTHNPNATIPITIWWLTPQLILLGVSSVFTMV 491
           VGMFISTLSMVVAALVE KRLAVAREHGLT NPN+TIPITIWWLTPQL+LLGVSSVFTMV
Sbjct: 465 VGMFISTLSMVVAALVEVKRLAVAREHGLTQNPNSTIPITIWWLTPQLLLLGVSSVFTMV 524

Query: 492 GLQEFFYDQVLIELKSVGLALYLSIFGVGNLLSGVLVSIIEDTT-GRHGRGGWLANNINN 551
           GLQEFFYDQV  E+KSVGLALYLSIFGVGNLLS +LVS+IE+ T G  GRGGW ANNIN 
Sbjct: 525 GLQEFFYDQVSSEVKSVGLALYLSIFGVGNLLSSLLVSVIEEATGGDDGRGGWFANNINK 584

Query: 552 AHLDYFYWLLAGIGEVGLLAYMYFANSYVYKYNVER 584
           AHLDYFYWLLAGIG+VGLLAY+YFANSYVYKYNVER
Sbjct: 585 AHLDYFYWLLAGIGKVGLLAYVYFANSYVYKYNVER 600

BLAST of Lsi08G000170 vs. NCBI nr
Match: KAA0039219.1 (protein NRT1/ PTR FAMILY 5.10-like [Cucumis melo var. makuwa] >TYK00407.1 protein NRT1/ PTR FAMILY 5.10-like [Cucumis melo var. makuwa])

HSP 1 Score: 876.3 bits (2263), Expect = 1.5e-250
Identity = 470/576 (81.60%), Postives = 511/576 (88.72%), Query Frame = 0

Query: 12  ILNDDVVPGSVDHRERPSRRSESGRWRAAGLIIGVEIAERLAFFGISTNLVSYLTVEMGQ 71
           ILN D +PG        +RRS+SG WRAAG+IIGVEIAER AFFGISTNLVSYLTVEM Q
Sbjct: 45  ILN-DAIPG--------NRRSQSGGWRAAGVIIGVEIAERFAFFGISTNLVSYLTVEMEQ 104

Query: 72  SMAAAAKNVNLWIGTASLLPLLAASFADSFLGRYLTILLASALYILVNLLFPFLFLILIL 131
           SMA+AA+NVNLWIGTASLLPL+AASFADSFLGRYLTI+LASALYI        L L L+ 
Sbjct: 105 SMASAAQNVNLWIGTASLLPLVAASFADSFLGRYLTIILASALYI--------LGLGLLT 164

Query: 132 LNIIIINKLSTLRGSG---SAATGASSQPVPDADVVLFFSALYLVAFAQGGHKPCVQAFG 191
           L+ ++ +  S+ +GSG   SAATGASS+PV    VVLFFS+LYLVAFAQGGHKPC+QAFG
Sbjct: 165 LSAMLASP-SSFQGSGSAASAATGASSRPV--LHVVLFFSSLYLVAFAQGGHKPCLQAFG 224

Query: 192 CDQFDGEDPQECIAKCSFFNWWYFATTFGSFIALIILNYIQDNLGWGLGFGIPCISSVAA 251
           CDQFDGEDPQECIAKCSFFNWWYFATT GSFIALIIL+YIQDNLGW LGFGIPCISS+ A
Sbjct: 225 CDQFDGEDPQECIAKCSFFNWWYFATTLGSFIALIILSYIQDNLGWCLGFGIPCISSLVA 284

Query: 252 LVVFLLGTRTYRFVTVANNEEKTFMRIGRVFVNAARNWRTTSSDIAILEEGQDAMLYQRY 311
           L+VFLLGT+TYRFVT+AN EEK FMRIGRVF NAARNWRT SS+I I EEGQDAMLY+R 
Sbjct: 285 LLVFLLGTQTYRFVTIANYEEKPFMRIGRVFFNAARNWRTASSEIDISEEGQDAMLYRRC 344

Query: 312 GQLRFLNKALVARMDSNKDLKTCNIVEVEEAKGILRLIPIWTASLAYAIVLSQCSTFFVK 371
           GQLRFLNKALVA +DS+ D KTCNIVEVEEAKG+LRLIPIW ASL+YAIVLSQCSTFFVK
Sbjct: 345 GQLRFLNKALVAPVDSDDDGKTCNIVEVEEAKGVLRLIPIWIASLSYAIVLSQCSTFFVK 404

Query: 372 QASTMDRSISPTFKIPAATIQCFALIAVLLFLPIYDQLLVPIARIFTLKPSGISMLQRIG 431
           Q STMDRSI P+FKIPAATIQCF  IAV+LF+PIYD+LLVPIARIFTLKPSGISMLQRIG
Sbjct: 405 QGSTMDRSIRPSFKIPAATIQCFGCIAVVLFVPIYDRLLVPIARIFTLKPSGISMLQRIG 464

Query: 432 VGMFISTLSMVVAALVECKRLAVAREHGLTHNPNATIPITIWWLTPQLILLGVSSVFTMV 491
           VGMFISTLSMVVAALVE KRLAVAREHGLT NPN+TIPITIWWLTPQL+LLGVSSVFTMV
Sbjct: 465 VGMFISTLSMVVAALVEVKRLAVAREHGLTQNPNSTIPITIWWLTPQLLLLGVSSVFTMV 524

Query: 492 GLQEFFYDQVLIELKSVGLALYLSIFGVGNLLSGVLVSIIEDTT-GRHGRGGWLANNINN 551
           GLQEFFYDQV  E+KSVGLALYLSIFGVGNLLS +LVS+IE+ T G  GRGGW ANNIN 
Sbjct: 525 GLQEFFYDQVSSEVKSVGLALYLSIFGVGNLLSSLLVSVIEEATGGDDGRGGWFANNINK 584

Query: 552 AHLDYFYWLLAGIGEVGLLAYMYFANSYVYKYNVER 584
           AHLDYFYWLLAGIG+VGLLAY+YFANSYVYKYNVER
Sbjct: 585 AHLDYFYWLLAGIGKVGLLAYVYFANSYVYKYNVER 600

BLAST of Lsi08G000170 vs. TAIR 10
Match: AT1G22540.1 (Major facilitator superfamily protein )

HSP 1 Score: 605.9 bits (1561), Expect = 3.4e-173
Identity = 320/574 (55.75%), Postives = 405/574 (70.56%), Query Frame = 0

Query: 5   DESRRPLILNDDVVPGSVDHRERPSRRSESGRWRAAGLIIGVEIAERLAFFGISTNLVSY 64
           DE+  PL+        +VD+R +P+ +S SG WR+AG IIGVE+AER A++GIS+NL++Y
Sbjct: 8   DEAGTPLL------AVTVDYRNKPAVKSSSGGWRSAGFIIGVEVAERFAYYGISSNLITY 67

Query: 65  LTVEMGQSMAAAAKNVNLWIGTASLLPLLAASFADSFLGRYLTILLASALYILVNLLFPF 124
           LT  +GQS AAAA NVN W GTASLLPLL A  ADSFLGR+ TIL ASALYI        
Sbjct: 68  LTGPLGQSTAAAAANVNAWSGTASLLPLLGAFVADSFLGRFRTILAASALYI-------- 127

Query: 125 LFLILILLNIIIINKLSTLRGSGSAATGASSQPVPDADVVLFFSALYLVAFAQGGHKPCV 184
                + L ++    LS +  S    +   S   P   V+ FFSALYLVA AQGGHKPCV
Sbjct: 128 -----VGLGVL---TLSAMIPSDCKVSNLLSSCSPRFQVITFFSALYLVALAQGGHKPCV 187

Query: 185 QAFGCDQFDGEDPQECIAKCSFFNWWYFATTFGSFIALIILNYIQDNLGWGLGFGIPCIS 244
           QAFG DQFD ++P+EC AK SFFNWWYF   FG+   L +LNYIQDNL W LGFGIPCI+
Sbjct: 188 QAFGADQFDEKEPEECKAKSSFFNWWYFGMCFGTLTTLWVLNYIQDNLSWALGFGIPCIA 247

Query: 245 SVAALVVFLLGTRTYRFVTVANNEEKTFMRIGRVFVNAARNWRTTSSDIAILEEGQDAML 304
            V ALVV LLGT TYRF ++   ++  F+RIG V+V A +NW  ++ D+A  EE    + 
Sbjct: 248 MVVALVVLLLGTCTYRF-SIRREDQSPFVRIGNVYVAAVKNWSVSALDVAAAEERLGLVS 307

Query: 305 YQRYGQLRFLNKALVARMDSNKDLKTCNIVEVEEAKGILRLIPIWTASLAYAIVLSQCST 364
                Q  FLNKALVA+  S      C+I E+EEAK +LRL PIW   L YA+V +Q  T
Sbjct: 308 CSSSQQFSFLNKALVAKNGS------CSIDELEEAKSVLRLAPIWLTCLVYAVVFAQSPT 367

Query: 365 FFVKQASTMDRSISPTFKIPAATIQCFALIAVLLFLPIYDQLLVPIARIFTLKPSGISML 424
           FF KQ +TM+RSI+P +KI  AT+Q F  +++++F+PIYD++L+PIAR FT KP GI+ML
Sbjct: 368 FFTKQGATMERSITPGYKISPATLQSFISLSIVIFIPIYDRVLIPIARSFTHKPGGITML 427

Query: 425 QRIGVGMFISTLSMVVAALVECKRLAVAREHGLTHNPNATIPITIWWLTPQLILLGVSSV 484
           QRIG G+F+S L+MVVAALVE KRL  A ++GL  +P+AT+P+++WWL PQ +L G++ V
Sbjct: 428 QRIGTGIFLSFLAMVVAALVEMKRLKTAADYGLVDSPDATVPMSVWWLVPQYVLFGITDV 487

Query: 485 FTMVGLQEFFYDQVLIELKSVGLALYLSIFGVGNLLSGVLVSIIEDTTGRHGRGGWLANN 544
           F MVGLQEFFYDQV  EL+SVGLALYLSIFG+GN LS  ++SIIE  T + G+  W ANN
Sbjct: 488 FAMVGLQEFFYDQVPNELRSVGLALYLSIFGIGNFLSSFMISIIEKATSQSGQASWFANN 547

Query: 545 INNAHLDYFYWLLAGIGEVGLLAYMYFANSYVYK 579
           +N AHLDYFYWLLA +  +GL +Y+Y A SYV K
Sbjct: 548 LNQAHLDYFYWLLACLSFIGLASYLYVAKSYVSK 552

BLAST of Lsi08G000170 vs. TAIR 10
Match: AT1G72140.1 (Major facilitator superfamily protein )

HSP 1 Score: 531.9 bits (1369), Expect = 6.3e-151
Identity = 288/571 (50.44%), Postives = 380/571 (66.55%), Query Frame = 0

Query: 12  ILNDDVVPGSVDHRERPSRRSESGRWRAAGLIIGVEIAERLAFFGISTNLVSYLTVEMGQ 71
           I++++ V  SVD R  PS RS SG W+++G  +  E+AE+ A+FGI++NL++Y T  +G+
Sbjct: 13  IVSNENVEFSVDFRGNPSIRSSSGAWKSSGFTMCAEVAEKFAYFGIASNLITYFTEALGE 72

Query: 72  SMAAAAKNVNLWIGTASLLPLLAASFADSFLGRYLTILLASALYILVNLLFPFLFLILIL 131
           S A AA NVNLW+GTA+ LPL+  S ADSFLGR+ TILL S+ YI+   L  F   I  L
Sbjct: 73  STAVAASNVNLWLGTAAFLPLIWGSIADSFLGRFRTILLTSSFYIMGLGLLTFSATIPSL 132

Query: 132 LNIIIINKLSTLRGSGSAATGASSQPVPDADVVLFFSALYLVAFAQGGHKPCVQAFGCDQ 191
            N                 T  S   V    V++FF ALYL+A  +GG K C++AFG DQ
Sbjct: 133 CN--------------DQETRESC--VSQVKVIIFFCALYLIALGEGGFKVCLRAFGADQ 192

Query: 192 FDGEDPQECIAKCSFFNWWYFATTFGSFIALIILNYIQDNLGWGLGFGIPCISSVAALVV 251
           FD +DP E  AK S+FNW YFA + G     ++ NY+Q+NL W LG+ IPC+S + AL +
Sbjct: 193 FDEQDPNESKAKSSYFNWLYFAISIGILTTRLVTNYVQENLSWALGYAIPCLSMMLALFL 252

Query: 252 FLLGTRTYRFVT-----VANNEEKTFMRIGRVFVNAARNWRTTSSDIAILEEGQDAMLYQ 311
           FLLG +TYRF T          +  F+RIGRVFV AARN R T SD  +L   +      
Sbjct: 253 FLLGIKTYRFSTGGEGRQGKKHDNPFVRIGRVFVAAARNRRQTPSDTCLLLPNESTK--- 312

Query: 312 RYGQLRFLNKALVARMDSNKDLKTCNIVEVEEAKGILRLIPIWTASLAYAIVLSQCSTFF 371
              + RFL++A++          +C+  EVEEAK +L LIPIW  SL + IV +Q  TFF
Sbjct: 313 ---KFRFLDRAVI----------SCDSYEVEEAKAVLSLIPIWLCSLVFGIVFAQSPTFF 372

Query: 372 VKQASTMDRSISPTFKIPAATIQCFALIAVLLFLPIYDQLLVPIARIFTLKPSGISMLQR 431
            KQ STMDRSIS T ++PAAT+QCF  +A+L+F+PIYD+L VPIAR  T KP+GI+ LQR
Sbjct: 373 TKQGSTMDRSISSTLQVPAATLQCFISLAILVFIPIYDRLFVPIARSITRKPAGITTLQR 432

Query: 432 IGVGMFISTLSMVVAALVECKRLAVAREHGLTHNPNATIPITIWWLTPQLILLGVSSVFT 491
           I  G+F+S +SMV+AALVE KRL  AR+HGL  +P AT+P+++ WL PQ IL GVS VFT
Sbjct: 433 ISTGIFLSIISMVIAALVEMKRLKTARDHGLVDSPKATVPMSVCWLIPQYILFGVSDVFT 492

Query: 492 MVGLQEFFYDQVLIELKSVGLALYLSIFGVGNLLSGVLVSIIEDTTGRHGRGGWLANNIN 551
           MVGLQEFFY +V  +L+S+GLALYLSI G+GN LS  +VS+IE+ T + G+  W +NN+N
Sbjct: 493 MVGLQEFFYGEVPPQLRSMGLALYLSIIGIGNFLSSFMVSVIEEATSQSGQVSWFSNNLN 551

Query: 552 NAHLDYFYWLLAGIGEVGLLAYMYFANSYVY 578
            AHLDYFYWLLA +  +  +  +YFA SY+Y
Sbjct: 553 QAHLDYFYWLLACLSSLAFIFTVYFAKSYLY 551

BLAST of Lsi08G000170 vs. TAIR 10
Match: AT1G72125.1 (Major facilitator superfamily protein )

HSP 1 Score: 514.6 bits (1324), Expect = 1.0e-145
Identity = 277/567 (48.85%), Postives = 379/567 (66.84%), Query Frame = 0

Query: 13  LNDDVVPGSVDHRERPSRRSESGRWRAAGLIIGVEIAERLAFFGISTNLVSYLTVEMGQS 72
           L ++ V  +VDHR   +RRS +GRWRAA  IIGVE+AER A +GI +NL+SYLT  +GQS
Sbjct: 9   LQEEYVIDAVDHRGFSARRSITGRWRAAWFIIGVEVAERFANYGIGSNLISYLTGPLGQS 68

Query: 73  MAAAAKNVNLWIGTASLLPLLAASFADSFLGRYLTILLASALYILVNLLFPFLFLILILL 132
            A AA NVN W G +++LPLL A  AD+FLGRY+TI++AS +Y+        L L  + L
Sbjct: 69  TAVAAANVNAWSGISTILPLLGAFVADAFLGRYITIIIASFIYV--------LGLAFLTL 128

Query: 133 NIIIINKLSTLRGSGSAATGASSQPVPDADVVLFFSALYLVAFAQGGHKPCVQAFGCDQF 192
           +  +I   + +  S S+   A           LFF +LYLVA  Q GHKPCVQAFG DQF
Sbjct: 129 SAFLIPNNTEVTSSPSSFLNA-----------LFFFSLYLVAIGQSGHKPCVQAFGADQF 188

Query: 193 DGEDPQECIAKCSFFNWWYFATTFGSFIALIILNYIQDNLGWGLGFGIPCISSVAALVVF 252
           D ++PQE   + SFFNWWY +   G  +A++++ YIQ+N+ W LGFGIPC+  V +LV+F
Sbjct: 189 DEKNPQENSDRSSFFNWWYLSMCAGIGLAILVVVYIQENVSWALGFGIPCVFMVISLVLF 248

Query: 253 LLGTRTYRFVTVANNEE-KTFMRIGRVFVNAARNWRTTSSDIAILEEGQDAMLYQRYGQL 312
           +LG ++YRF      EE   F RIGRVF  A +N R  SSD+  +E  +     +   +L
Sbjct: 249 VLGRKSYRFSKTRQEEETNPFTRIGRVFFVAFKNQRLNSSDLCKVELIEANRSQESPEEL 308

Query: 313 RFLNKALVARMDSNKDLKTCNIVEVEEAKGILRLIPIWTASLAYAIVLSQCSTFFVKQAS 372
            FLNKAL+   DS++    C   +VE+A  ++RLIP+W  +LAYAI  +Q  TFF KQ  
Sbjct: 309 SFLNKALLVPNDSDEGEVACKSRDVEDATALVRLIPVWLTTLAYAIPFAQYMTFFTKQGV 368

Query: 373 TMDRSISPTFKIPAATIQCFALIAVLLFLPIYDQLLVPIARIFTLKPSGISMLQRIGVGM 432
           TM+R+I P  +IP A++Q    I+++LF+PIYD++LVPI R  T  P GI+ L+RIG GM
Sbjct: 369 TMERTIFPGVEIPPASLQVLISISIVLFVPIYDRVLVPIGRSITKDPCGITTLKRIGTGM 428

Query: 433 FISTLSMVVAALVECKRLAVAREHGLTHNPNATIPITIWWLTPQLILLGVSSVFTMVGLQ 492
            ++TL+MVVAALVE KRL  A+E+GL   P  T+P++IWWL PQ +LLG++ V T+VG+Q
Sbjct: 429 VLATLTMVVAALVESKRLETAKEYGLIDQPKTTLPMSIWWLFPQYMLLGLADVHTLVGMQ 488

Query: 493 EFFYDQVLIELKSVGLALYLSIFGVGNLLSGVLVSIIEDTTGRHGRGGWLANNINNAHLD 552
           EFFY QV  EL+S+GLA+YLS  GVG+LLS +L+ +I+  TG      W  +N+N AHLD
Sbjct: 489 EFFYSQVPTELRSLGLAIYLSAMGVGSLLSSLLIYLIDLATGGDAGNSWFNSNLNRAHLD 548

Query: 553 YFYWLLAGIGEVGLLAYMYFANSYVYK 579
           YFYWLLA +  VG   +++ + SY+Y+
Sbjct: 549 YFYWLLAVVSAVGFFTFLFISKSYIYR 556

BLAST of Lsi08G000170 vs. TAIR 10
Match: AT1G72120.1 (Major facilitator superfamily protein )

HSP 1 Score: 513.5 bits (1321), Expect = 2.3e-145
Identity = 275/567 (48.50%), Postives = 378/567 (66.67%), Query Frame = 0

Query: 13  LNDDVVPGSVDHRERPSRRSESGRWRAAGLIIGVEIAERLAFFGISTNLVSYLTVEMGQS 72
           L ++ V  +VDHR   +RRS +GRWRAA  IIGVE+AER A++GI +NL+SYLT  +G+S
Sbjct: 9   LQEEYVTDAVDHRGLAARRSNTGRWRAALFIIGVEVAERFAYYGIGSNLISYLTGPLGES 68

Query: 73  MAAAAKNVNLWIGTASLLPLLAASFADSFLGRYLTILLASALYILVNLLFPFLFLILILL 132
            A AA NVN W G A+LLP+L A  AD+FLGRY TI+++S +Y+        L L  + L
Sbjct: 69  TAVAAANVNAWSGIATLLPVLGAFVADAFLGRYRTIIISSLIYV--------LGLAFLTL 128

Query: 133 NIIIINKLSTLRGSGSAATGASSQPVPDADVVLFFSALYLVAFAQGGHKPCVQAFGCDQF 192
           +  +I   + +  S S+              VLFF +LYLVA  Q GHKPCVQAFG DQF
Sbjct: 129 SAFLIPNTTEVTSSTSSFLN-----------VLFFFSLYLVAIGQSGHKPCVQAFGADQF 188

Query: 193 DGEDPQECIAKCSFFNWWYFATTFGSFIALIILNYIQDNLGWGLGFGIPCISSVAALVVF 252
           D +D QE   + SFFNWWY + + G   A++++ YIQ+   W  GFGIPC+  V +LV+F
Sbjct: 189 DEKDSQEKSDRSSFFNWWYLSLSAGICFAILVVVYIQEEFSWAFGFGIPCVFMVISLVLF 248

Query: 253 LLGTRTYRFVTVANNEE-KTFMRIGRVFVNAARNWRTTSSDIAILEEGQDAMLYQRYGQL 312
           + G R YR+    + EE   F RIGRVF  A +N R +SSD+  +E   +    ++    
Sbjct: 249 VSGRRIYRYSKRRHEEEINPFTRIGRVFFVALKNQRLSSSDLCKVELEANTSPEKQ---- 308

Query: 313 RFLNKALVARMDSNKDLKTCNIVEVEEAKGILRLIPIWTASLAYAIVLSQCSTFFVKQAS 372
            F NKAL+   DS++        +VE+A  ++RLIP+W  +LAYAI  +Q  TFF KQ  
Sbjct: 309 SFFNKALLVPNDSSQGENASKSSDVEDATALIRLIPVWFTTLAYAIPYAQYMTFFTKQGV 368

Query: 373 TMDRSISPTFKIPAATIQCFALIAVLLFLPIYDQLLVPIARIFTLKPSGISMLQRIGVGM 432
           TMDR+I P  KIP A++Q F  I+++LF+PIYD++ VPIAR+ T +P GI+ L+RIG G+
Sbjct: 369 TMDRTILPGVKIPPASLQVFIGISIVLFVPIYDRVFVPIARLITKEPCGITTLKRIGTGI 428

Query: 433 FISTLSMVVAALVECKRLAVAREHGLTHNPNATIPITIWWLTPQLILLGVSSVFTMVGLQ 492
            +ST++MV+AALVE KRL  A+EHGL   P AT+P++IWWL PQ +LLG++ V+T+VG+Q
Sbjct: 429 VLSTITMVIAALVEFKRLETAKEHGLIDQPEATLPMSIWWLIPQYLLLGLADVYTLVGMQ 488

Query: 493 EFFYDQVLIELKSVGLALYLSIFGVGNLLSGVLVSIIEDTTGRHGRGGWLANNINNAHLD 552
           EFFY QV  EL+S+GLALYLS  GVG+LLS +L+S+I+  TG      W  +N+N AHLD
Sbjct: 489 EFFYSQVPTELRSIGLALYLSALGVGSLLSSLLISLIDLATGGDAGNSWFNSNLNRAHLD 548

Query: 553 YFYWLLAGIGEVGLLAYMYFANSYVYK 579
           YFYWLLA +  VG   +++ + SY+Y+
Sbjct: 549 YFYWLLAIVSAVGFFTFLFISKSYIYR 552

BLAST of Lsi08G000170 vs. TAIR 10
Match: AT1G22550.1 (Major facilitator superfamily protein )

HSP 1 Score: 502.3 bits (1292), Expect = 5.3e-142
Identity = 276/568 (48.59%), Postives = 380/568 (66.90%), Query Frame = 0

Query: 13  LNDDVVPGSVDHRERPSRRSESGRWRAAGLIIGVEIAERLAFFGISTNLVSYLTVEMGQS 72
           L +D V  SVDHR  P+ +S +G WR+A  IIGVE+ ER A+FGI +NL++YLT  +GQS
Sbjct: 10  LIEDSVSDSVDHRGLPAGKSSTGGWRSAWYIIGVEVGERFAYFGIGSNLITYLTGPLGQS 69

Query: 73  MAAAAKNVNLWIGTASLLPLLAASFADSFLGRYLTILLASALYILVNLLFPFLFLILILL 132
            A AA NVN W GTAS+LP+L A  AD++LGRY TI++AS +YIL          +L L 
Sbjct: 70  TATAAVNVNTWSGTASILPVLGAFIADAYLGRYRTIVVASLIYILG-------LGLLTLS 129

Query: 133 NIIIINKLSTLRGSGSAATGASSQPVPDADVVLFFSALYLVAFAQGGHKPCVQAFGCDQF 192
           +I+I+  LS  R        AS++P    + +LFF +LYLVA  QGGHKPCVQAFG DQF
Sbjct: 130 SILILMGLSEQRQHN---RNASAKPFFWVN-ILFFCSLYLVAIGQGGHKPCVQAFGADQF 189

Query: 193 DGEDPQECIAKCSFFNWWYFATTFGSFIALIILNYIQDNLGWGLGFGIPCISSVAALVVF 252
           D  DP+E I++ SFFNWW+ + + G  +++I++ Y+QDN+ W LGFGIPC+  V AL +F
Sbjct: 190 DVGDPKERISRGSFFNWWFLSLSAGITLSIIVVVYVQDNVNWALGFGIPCLFMVMALALF 249

Query: 253 LLGTRTYRFVT-VANNEEKTFMRIGRVFVNAARNWRTTSSDIAILEEGQDAMLYQR-YGQ 312
           L G +TYR+       +   F RIGRVF+ A +N +   +    LE G     Y++  GQ
Sbjct: 250 LFGRKTYRYPRGDREGKNNAFARIGRVFLVAFKNRKLKLTHSGQLEVGS----YKKCKGQ 309

Query: 313 LRFLNKALVARMDSNKDLKTCNIVEVEEAKGILRLIPIWTASLAYAIVLSQCSTFFVKQA 372
           L FL KAL   +     ++ C+  +VE+A  ++RLIPIW  S+   I  +Q +TFF KQ 
Sbjct: 310 LEFLAKAL---LPGEGGVEPCSSRDVEDAMALVRLIPIWITSVVSTIPYAQYATFFTKQG 369

Query: 373 STMDRSISPTFKIPAATIQCFALIAVLLFLPIYDQLLVPIARIFTLKPSGISMLQRIGVG 432
            T+DR I P F+IP A+ Q    +++ + +P Y+++ +P+AR+ T KPSGI+MLQRIG G
Sbjct: 370 VTVDRKILPGFEIPPASFQALIGLSIFISVPTYERVFLPLARLITKKPSGITMLQRIGAG 429

Query: 433 MFISTLSMVVAALVECKRLAVAREHGLTHNPNATIPITIWWLTPQLILLGVSSVFTMVGL 492
           M +S+L+MVVAALVE KRL  A+EHGL   P+ATIP++IWW  PQ +LLG+  VF++VG 
Sbjct: 430 MVLSSLNMVVAALVEMKRLETAKEHGLVDRPDATIPMSIWWFVPQYLLLGMIDVFSLVGT 489

Query: 493 QEFFYDQVLIELKSVGLALYLSIFGVGNLLSGVLVSIIEDTTGRHGRGGWLANNINNAHL 552
           QEFFYDQV  EL+S+GLAL LS  G+ + LSG L+++I   TG++G   W   N+N AH+
Sbjct: 490 QEFFYDQVPTELRSIGLALSLSAMGLASFLSGFLITVINWATGKNGGDSWFNTNLNRAHV 549

Query: 553 DYFYWLLAGIGEVGLLAYMYFANSYVYK 579
           DYFYWLLA    +G LA++  +  YVY+
Sbjct: 550 DYFYWLLAAFTAIGFLAFLLLSRLYVYR 559

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q0WP014.8e-17255.75Protein NRT1/ PTR FAMILY 5.10 OS=Arabidopsis thaliana OX=3702 GN=NPF5.10 PE=2 SV... [more]
Q9C7U18.8e-15050.44Protein NRT1/ PTR FAMILY 5.12 OS=Arabidopsis thaliana OX=3702 GN=NPF5.12 PE=2 SV... [more]
Q0WSZ61.5e-14448.85Protein NRT1/ PTR FAMILY 5.13 OS=Arabidopsis thaliana OX=3702 GN=NPF5.13 PE=2 SV... [more]
Q8VZE23.2e-14448.50Protein NRT1/ PTR FAMILY 5.14 OS=Arabidopsis thaliana OX=3702 GN=NPF5.14 PE=2 SV... [more]
Q9SK967.5e-14148.59Protein NRT1/ PTR FAMILY 5.16 OS=Arabidopsis thaliana OX=3702 GN=NPF5.16 PE=2 SV... [more]
Match NameE-valueIdentityDescription
A0A5D3BMJ37.0e-25181.60Protein NRT1/ PTR FAMILY 5.10-like OS=Cucumis melo var. makuwa OX=1194695 GN=E56... [more]
A0A1S4E2H87.0e-25181.42protein NRT1/ PTR FAMILY 5.10-like OS=Cucumis melo OX=3656 GN=LOC107991676 PE=3 ... [more]
A0A6J1DMG41.8e-20970.79uncharacterized protein LOC111022389 isoform X2 OS=Momordica charantia OX=3673 G... [more]
A0A5B6YPP02.1e-18658.98Uncharacterized protein OS=Davidia involucrata OX=16924 GN=Din_003158 PE=3 SV=1[more]
A0A5B6YQ376.1e-18658.80Uncharacterized protein OS=Davidia involucrata OX=16924 GN=Din_003159 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_038891003.14.1e-26984.59protein NRT1/ PTR FAMILY 5.10-like [Benincasa hispida][more]
KAE8649018.11.2e-25283.30hypothetical protein Csa_009254 [Cucumis sativus][more]
XP_011656124.21.2e-25283.30uncharacterized protein LOC105435629 [Cucumis sativus][more]
XP_016902428.11.5e-25081.42PREDICTED: protein NRT1/ PTR FAMILY 5.10-like [Cucumis melo][more]
KAA0039219.11.5e-25081.60protein NRT1/ PTR FAMILY 5.10-like [Cucumis melo var. makuwa] >TYK00407.1 protei... [more]
Match NameE-valueIdentityDescription
AT1G22540.13.4e-17355.75Major facilitator superfamily protein [more]
AT1G72140.16.3e-15150.44Major facilitator superfamily protein [more]
AT1G72125.11.0e-14548.85Major facilitator superfamily protein [more]
AT1G72120.12.3e-14548.50Major facilitator superfamily protein [more]
AT1G22550.15.3e-14248.59Major facilitator superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000109Proton-dependent oligopeptide transporter familyPFAMPF00854PTR2coord: 107..536
e-value: 3.4E-82
score: 276.4
IPR000109Proton-dependent oligopeptide transporter familyPANTHERPTHR11654OLIGOPEPTIDE TRANSPORTER-RELATEDcoord: 21..580
IPR036259MFS transporter superfamilyGENE3D1.20.1250.20MFS general substrate transporter like domainscoord: 10..583
e-value: 8.1E-171
score: 570.8
IPR036259MFS transporter superfamilySUPERFAMILY103473MFS general substrate transportercoord: 23..572
NoneNo IPR availablePANTHERPTHR11654:SF510FACILITATOR SUPERFAMILY PROTEIN, PUTATIVE ISOFORM 1-RELATEDcoord: 21..580
IPR044739NRT1/PTR familyCDDcd17417MFS_NPF5coord: 37..577
e-value: 0.0
score: 604.261

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi08G000170.1Lsi08G000170.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0035442 dipeptide transmembrane transport
biological_process GO:0042939 tripeptide transport
biological_process GO:0042938 dipeptide transport
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0016020 membrane
molecular_function GO:0071916 dipeptide transmembrane transporter activity
molecular_function GO:0042937 tripeptide transmembrane transporter activity
molecular_function GO:0022857 transmembrane transporter activity