Lsi07G004110 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi07G004110
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionDefective in cullin neddylation protein
Locationchr07: 4425457 .. 4430399 (-)
RNA-Seq ExpressionLsi07G004110
SyntenyLsi07G004110
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TTTATTTTTGGAATACTTGAAAGAGAGAGAATTAGGAAGGAAGATAAAATGTAGTAATCAAAATAAAATATAAAATCTAAAGTTGTAATTGTAAACCTACATTATATAATTTGAGAAAATCAAGAAAAGAAGGAAATTAGAAGACGATAGGCCGAAGTTTTGCTATTCTCCAATTTGCTGCGACAAAAATCAATCTTCCTTCTTTGCTTCTCTTCTTATCTCCTCCATCGTAATCGAAGTTCTCAAGAAGAAGAAGAAGATGCGGCGCTCTGCTACAAGGAAAACCGGTCAACCCAATTCAACTTCTGTTAATTCTTCCGCCGTCGATCTCTTTCGCTCCGGTATTTGATCTTTTTTCTTTTTCACAATTCGAATTCTTCTACTAGTAATTCTCACCATGGAAATCGATGATCGTGTTTTGTATATATTGTGGTTAATTCTGTGCTCTGTGGTCATGGACATTGTTTGTCTAATCAATTACTCCCATGATGCCTTTATCTGAGGAGATTTTTATGCTTTCGATGTTTTGTGCGCATCTTATTATGTTGATGATTTAGTTAAGTTTTTACATAAGTGGGATTTCAGTAGGATGAATAACTCTAGTTTTTAGCTAATATTGTGTTTACGGCTTTGAAATTTGGGAATTTCAAGCTTATATTACAGATTGTTTCGTACATGTACTTGAGCTCCTGTTAGAGTTGATCCGAGATGACATGAAGTGAGTTAAAGGTTTCATTAGAAAGTGAGAGTTGTAGAGTTAGAAATTAAAAGCAAGAGAATCTGAACATGCAAACTATTTTTATTCATCGTATTGAGATTAAAAAAAAAAATAAATTCAGTTATGAACTCTGTTGGGTGGTCTAATGGCTGTTCTTAAGCAAAACTATGTGGTTGGTGGCTTTCTCAGTTATATCCTAATAATTATCATGCCCATGTTGAGGTGTACTCTTTTGTTCTTATGAGGTGATTTATGTGAAAGTGTTGATTTTTTAGATTTCTTCCACTATATTAGCCCCTCTTCTTACTATATAAAAATATAAGTTCTCAATTCTTATGAACTTAAGATTCTCAATTTATTCAGCCTCAAGTAGAGCAAGCACAAAAGAGTTGGAACGGATCGATAACCTATTTTACTCATATGCCAATGGCTCTTCTGGTTTGATCGAGTAAGTCAACTTTGGTCAATTGAAATAACTCATTTTTCAGCATTGCTAGTTAGCTTTGCATTGTACCGTATATATTTTTTAATTCAGTATTATACTGTTTAAATTCCTAGCAACGTTCAAATTACAAACTTCCATCCATGTTCCTTTAATGATCAAGGAGGCTGTTCTTCTTATCTTTAAGGAGACTTTTGAATGCTTGTATGAATAAGATCCGTATGGTATGTAGTAATGACTATTTCTTTAACAGTCCAGAAGGAATTGAAAGTCTTTGTTCAGACATGGAAGTGAATCACACTGATGTGAGGATCTTGATGCTTGCTTGGTAAGTCGTACTTAGAATCTATTGAAAGCTCAAGTTTTTCTGGTTTATATGACCTCATTGCCTGGGAACTAATGGATTGCACATACCAGGAAAATGAAATCTGAAAAACAAGGATACTTTAACCTGGTAAGAATATTTACTCTCTTGTGGGCCTCAGAATATTACAAATTATACATCAGATCTTCTTTCTAGTATTGCTGTCCTTTGATTTACCACTGTATGCATCACATCAGTTAATCCTTTCTCTTTCATTTTCTAACCAGTTTAGTAATCTATGAGGAGTATATTTTATTTTATCCAAGTGGTGAGTCTTTATTAACTCTTTGGAGTGGGGTTCTAACCCCCTTTCTCGTGTAATTTCATACCATCGAGGAATTGTTTTTTATAAAAAAAAAAAAAAAAAAAAAAGAAAAGAACATCCATTTTTATCTGTGTTCAGCACCCGCTTATAAGGAAAATCCTGTCATTTATATTCTCAGCTTCAATTTAGAAAATTTGGTTGCCAAGATCTTCGAATTGTTTACATTTTATTTTTCATGCCTTTTCTTCATGTCTATTTCCCATCTTATCTATTTTAATTGCATTGTGAAGACAAGATGAAGCATTTAGTCACTCTTTACTAAATTCATTTGATGGAACATTAACCTGTTGTATATTTGATAGGAAGAATGGCGCACAGGCCTCAAATCCCTGAGGGCTGACACTGTTAGTAAATTAAAGAAGGCACTACCAGATCTCGAGAAAGAGGTACAATACCTGTCTTGTTAAGAGTTTCAAAACAAATTAATACATATAGTTAGTGCATTGCTTAATTTTGGGCATAAAGTTTTTAGTGACCAATTCCTCACATTTTGAGAAGGTAAGGAGGCCGTCAAACTTTGTGGACTTCTATTCTTATGCATTTCGGTATTGTTTAACAGGTAAACAAATATTGTTTAGTGTATTTTTAATCTTGAAGGTTGCTTCATTTTCTTCTATCCTATATTGTAATTTGATCTTTACGTTCTCTTTATTCCTTTGATATTTTTCTTAGAGGAGAAGCAAAAGAGTATTGACATAGAGAGTATATGCGAGTTACTGGATCTTGTTTTAGGGTCTCAGTTTCATCCCCAAGTCAATGCATTTGTTGAATACTTGAAGGTGAGTGCCGCCTTGTGTTTTTTGTCTTTTAAATTCCACATTTACCCACAGGCATGATTTATTTGGCACTGCAGTTAACTCCAGCACTGCTTTATAGTACTTCTAGGCGTTTAATAGTAGCACTAGCTCTTAGTCTAAGAGAGTTACACTCTTGAGTTATAGCCTATCTCTAGCATACCTAGTGGAGCATACTGCTCAGTGTTGATAACTGGACTTGACCATATTATTACGGGTAGTCATAGCTGCAGCCACCTAGTTAATCTGCTTTCTCATGAGAATCTTAGGATTTAGGACATGGTTGGTGATTCGAGACTGGTGATTCTGTAAAAGACATGCTCCAGAGTAATGAATTTTCTTACATAGGATTTCAACTTTTTGTATTATTGACCGATTAATCCCATGTTTGCTGCCTTCCCCCTCATCAAGAAGGTAGAATGAAAATTTCTTATTTGCTCGTACTTTGCATGCAGATACAAAGTGACTATAAGGTTATTAACATGGATCAATGGATGGGCTTCTTTCGATTCTGCAATGAGGTACTTTCATTATCTCCCATAGTACATTAACACGACACGTTTCATTATATTTGCTTGCATCTGTTGCACTACCAGTGGAATACAGTAAGAAAAATTGAGAGGAAAATACTTCTGGAATCTACTATGAACATGCTTTGAATTTTACAAGAGAGTCATTGCCAATTCAATTATCTTCTGATCCCTTGAATTCAATAAAACAAGTATGATCTTAAGCTTGATAACTATGTGGATTTTGAAAATTAAGCTTATAAACACTATTTCCACTACAAGTTCAATATTTTGTTGTCCATTTTCTACCAATGTTTTTCAAACCAAATTTGACTAGGAATCTTTCTTAAAGGAAGATGAAAACTATAATTGAACAATTGGGAGGCAACAAACATAAATTTCAAAAACAGAAAACTAAAACAAAATCATTATCAAATAGCCTAAGTTTGTGAGTTATCTGAATTCAAAGTTTTTGAGTTAACTGAATTCTTTTCTTTTTTCAATTGGTCTAGAAAATGTGTATTTGGATGATCCAAAGGGTTAAATACTTACTAGTATTTTTCTCGTACTCTGTTATTATTCTTCTTTTGAACCTGTAGATATGGAAAATGAATAAATTTTAATTGAAAAAAATCTAGAAAAAAGGATCCACCTCATACCATAATTCCCCTTGTATAGAAAATCAGTCTGAAGGAGAAGTGTAATATGATGCAAGAATAACTTCTCTATCCACCTCAAATTGATTTGTTTGTATCATCAGCATATGGTATTGATGTCCTCAATTCAAATTGTTGACACACTTGATGATCATTATGCAGATAAGTTTTCCCGAATTGAGCAATTACGACTCGGAACTCGCTTGGCCGTTAATCCTCGACAATTTTGTTGAATGGTTACAAGCAAAGCAGAGTTAAAAACCTGATGTGTTCATCATACAATGTATTTTGATGTATACGCCACAGTTGTTGAAGCAAGTATCCTAATCCTAATTTCTCTTACCGTTTCAGATTCAATATATATTTCTTCGTCCTTTCTAACCAGATTTATGAACATTTCTGTTTCTGCAGGCTCACCTTCGATTACACGTTTGCATAGCAGCAGGATTTTCACTCATTATTCCTTATTTTACTCTTTTAACTTCTTTGAAATCAATGGTCATTCCCATTATGTTCATTTCAAACATTCATGGATTATTCTTCTGCTAAATTATTGAAGTTTCCTACTGTGTTTTCGTTTCCTCCATTCTTCATCATGTTACTTAGCCATTCAATTGATATTGACTGACTATTTTGACAGACTTCTTTTGTGTATCCATGAAACAGCAGCTCAAGCTGAAATTTGCCCGACAGCAAGAATTTGGGAATATTTTTATTGGCTTTTTTGAATACTTGTCCCAAAAAAGAAAAGAAAAAAGAAAAAAAGAAAAAAAAAAGTAGTTTGCTGTTAAATTAGAGGTCCTAGGTGGTATCGTGTCCAATTAACTTTTTCCCCTCCCTCTTCTTCTTCTTCTTCTTTCCTTTTGTTAGGGTTCCTGTGAGTTATTATTGAGAATGACTAGAGTTACAGAAATTCAAATGCGTTTGTAACCTTTTATTGTTGTATATTTTTTTTTAGTTGTTTTCAAATATAGCAAAATGAGCTAACATATTTATAGATGTAGCAAAATGTCATTTTATATTAGTGATAGACAATGATAGACTACTATCATCTATCATGGTCTATCATTGATAGATAGTGACATTTTTGCTATATTTGAAAATATTTTCAGCAATTTTGCCATTTAAAACAAAA

mRNA sequence

TTTATTTTTGGAATACTTGAAAGAGAGAGAATTAGGAAGGAAGATAAAATGTAGTAATCAAAATAAAATATAAAATCTAAAGTTGTAATTGTAAACCTACATTATATAATTTGAGAAAATCAAGAAAAGAAGGAAATTAGAAGACGATAGGCCGAAGTTTTGCTATTCTCCAATTTGCTGCGACAAAAATCAATCTTCCTTCTTTGCTTCTCTTCTTATCTCCTCCATCGTAATCGAAGTTCTCAAGAAGAAGAAGAAGATGCGGCGCTCTGCTACAAGGAAAACCGGTCAACCCAATTCAACTTCTGTTAATTCTTCCGCCGTCGATCTCTTTCGCTCCGCCTCAAGTAGAGCAAGCACAAAAGAGTTGGAACGGATCGATAACCTATTTTACTCATATGCCAATGGCTCTTCTGGTTTGATCGATCCAGAAGGAATTGAAAGTCTTTGTTCAGACATGGAAGTGAATCACACTGATGTGAGGATCTTGATGCTTGCTTGGAAAATGAAATCTGAAAAACAAGGATACTTTAACCTGGAAGAATGGCGCACAGGCCTCAAATCCCTGAGGGCTGACACTGTTAGTAAATTAAAGAAGGCACTACCAGATCTCGAGAAAGAGGTAAGGAGGCCGTCAAACTTTGTGGACTTCTATTCTTATGCATTTCGGTATTGTTTAACAGAGGAGAAGCAAAAGAGTATTGACATAGAGAGTATATGCGAGTTACTGGATCTTGTTTTAGGGTCTCAGTTTCATCCCCAAGTCAATGCATTTGTTGAATACTTGAAGATACAAAGTGACTATAAGGTTATTAACATGGATCAATGGATGGGCTTCTTTCGATTCTGCAATGAGATAAGTTTTCCCGAATTGAGCAATTACGACTCGGAACTCGCTTGGCCGTTAATCCTCGACAATTTTGTTGAATGGTTACAAGCAAAGCAGAGTTAAAAACCTGATGTGTTCATCATACAATGTATTTTGATGTATACGCCACAGTTGTTGAAGCTCACCTTCGATTACACGTTTGCATAGCAGCAGGATTTTCACTCATTATTCCTTATTTTACTCTTTTAACTTCTTTGAAATCAATGGTCATTCCCATTATGTTCATTTCAAACATTCATGGATTATTCTTCTGCTAAATTATTGAAGTTTCCTACTGTGTTTTCGTTTCCTCCATTCTTCATCATGTTACTTAGCCATTCAATTGATATTGACTGACTATTTTGACAGACTTCTTTTGTGTATCCATGAAACAGCAGCTCAAGCTGAAATTTGCCCGACAGCAAGAATTTGGGAATATTTTTATTGGCTTTTTTGAATACTTGTCCCAAAAAAGAAAAGAAAAAAGAAAAAAAGAAAAAAAAAAGTAGTTTGCTGTTAAATTAGAGGTCCTAGGTGGTATCGTGTCCAATTAACTTTTTCCCCTCCCTCTTCTTCTTCTTCTTCTTTCCTTTTGTTAGGGTTCCTGTGAGTTATTATTGAGAATGACTAGAGTTACAGAAATTCAAATGCGTTTGTAACCTTTTATTGTTGTATATTTTTTTTTAGTTGTTTTCAAATATAGCAAAATGAGCTAACATATTTATAGATGTAGCAAAATGTCATTTTATATTAGTGATAGACAATGATAGACTACTATCATCTATCATGGTCTATCATTGATAGATAGTGACATTTTTGCTATATTTGAAAATATTTTCAGCAATTTTGCCATTTAAAACAAAA

Coding sequence (CDS)

ATGCGGCGCTCTGCTACAAGGAAAACCGGTCAACCCAATTCAACTTCTGTTAATTCTTCCGCCGTCGATCTCTTTCGCTCCGCCTCAAGTAGAGCAAGCACAAAAGAGTTGGAACGGATCGATAACCTATTTTACTCATATGCCAATGGCTCTTCTGGTTTGATCGATCCAGAAGGAATTGAAAGTCTTTGTTCAGACATGGAAGTGAATCACACTGATGTGAGGATCTTGATGCTTGCTTGGAAAATGAAATCTGAAAAACAAGGATACTTTAACCTGGAAGAATGGCGCACAGGCCTCAAATCCCTGAGGGCTGACACTGTTAGTAAATTAAAGAAGGCACTACCAGATCTCGAGAAAGAGGTAAGGAGGCCGTCAAACTTTGTGGACTTCTATTCTTATGCATTTCGGTATTGTTTAACAGAGGAGAAGCAAAAGAGTATTGACATAGAGAGTATATGCGAGTTACTGGATCTTGTTTTAGGGTCTCAGTTTCATCCCCAAGTCAATGCATTTGTTGAATACTTGAAGATACAAAGTGACTATAAGGTTATTAACATGGATCAATGGATGGGCTTCTTTCGATTCTGCAATGAGATAAGTTTTCCCGAATTGAGCAATTACGACTCGGAACTCGCTTGGCCGTTAATCCTCGACAATTTTGTTGAATGGTTACAAGCAAAGCAGAGTTAA

Protein sequence

MRRSATRKTGQPNSTSVNSSAVDLFRSASSRASTKELERIDNLFYSYANGSSGLIDPEGIESLCSDMEVNHTDVRILMLAWKMKSEKQGYFNLEEWRTGLKSLRADTVSKLKKALPDLEKEVRRPSNFVDFYSYAFRYCLTEEKQKSIDIESICELLDLVLGSQFHPQVNAFVEYLKIQSDYKVINMDQWMGFFRFCNEISFPELSNYDSELAWPLILDNFVEWLQAKQS
Homology
BLAST of Lsi07G004110 vs. ExPASy Swiss-Prot
Match: Q92564 (DCN1-like protein 4 OS=Homo sapiens OX=9606 GN=DCUN1D4 PE=1 SV=2)

HSP 1 Score: 148.7 bits (374), Expect = 8.2e-35
Identity = 84/205 (40.98%), Postives = 121/205 (59.02%), Query Frame = 0

Query: 29  SSRASTKE----LERIDNLFYSYANGSSGLIDPEGIESLCSDMEVNHTDVRILMLAWKMK 88
           S+R  T+E     +R    FY YA G+  ++ PEG+E  C D+ V   +V +L+LAWK+ 
Sbjct: 91  STRIKTEEEAFSSKRCLEWFYEYA-GTDDVVGPEGMEKFCEDIGVEPENVVMLVLAWKLD 150

Query: 89  SEKQGYFNLEEWRTGLKSLRADTVSKLKKALPDLEKEVRRPSNFVDFYSYAFRYCLTEEK 148
           ++  GYF L+EW  G+ SL+ DT  KL+  L  L   +   +NF   Y YAF +   E+ 
Sbjct: 151 AQNMGYFTLQEWLKGMTSLQCDTTEKLRNTLDYLRSFLNDSTNFKLIYRYAFDFA-REKD 210

Query: 149 QKSIDIESICELLDLVLGSQFHPQVNAFVEYLKIQSDYKVINMDQWMGFFRFCNEISFPE 208
           Q+S+DI +   +L L+LG +  P    F ++L+ QS YKVIN DQW     F   I+  +
Sbjct: 211 QRSLDINTAKCMLGLLLG-KIWPLFPVFHQFLE-QSKYKVINKDQWCNVLEFSRTINL-D 270

Query: 209 LSNYDSELAWPLILDNFVEWLQAKQ 230
           LSNYD + AWP++LD FVEW + KQ
Sbjct: 271 LSNYDEDGAWPVLLDEFVEWYKDKQ 290

BLAST of Lsi07G004110 vs. ExPASy Swiss-Prot
Match: Q8CCA0 (DCN1-like protein 4 OS=Mus musculus OX=10090 GN=Dcun1d4 PE=2 SV=1)

HSP 1 Score: 148.7 bits (374), Expect = 8.2e-35
Identity = 85/205 (41.46%), Postives = 120/205 (58.54%), Query Frame = 0

Query: 29  SSRASTKE----LERIDNLFYSYANGSSGLIDPEGIESLCSDMEVNHTDVRILMLAWKMK 88
           S+R  T+E     +R    FY YA G+   + PEG+E  C D+ V   +V +L+LAWK+ 
Sbjct: 91  STRIKTEEEAFSSKRCLEWFYEYA-GTEDAVGPEGMEKFCEDIGVEPENVVMLVLAWKLD 150

Query: 89  SEKQGYFNLEEWRTGLKSLRADTVSKLKKALPDLEKEVRRPSNFVDFYSYAFRYCLTEEK 148
           ++  GYF L+EW  G+ SL+ DT  KL+  L  L   +   +NF   Y YAF +   E+ 
Sbjct: 151 AQNMGYFTLQEWLKGMTSLQCDTTEKLRTTLDYLRSLLNDTTNFKLIYRYAFDFA-REKD 210

Query: 149 QKSIDIESICELLDLVLGSQFHPQVNAFVEYLKIQSDYKVINMDQWMGFFRFCNEISFPE 208
           Q+S+DI +   +L L+LG +  P    F ++L+ QS YKVIN DQW     F   IS  +
Sbjct: 211 QRSLDINTAKCMLGLLLG-KIWPLFPVFHQFLE-QSKYKVINKDQWCNVLEFSRTISL-D 270

Query: 209 LSNYDSELAWPLILDNFVEWLQAKQ 230
           LSNYD + AWP++LD FVEW + KQ
Sbjct: 271 LSNYDEDGAWPVLLDEFVEWYKDKQ 290

BLAST of Lsi07G004110 vs. ExPASy Swiss-Prot
Match: Q9BTE7 (DCN1-like protein 5 OS=Homo sapiens OX=9606 GN=DCUN1D5 PE=1 SV=1)

HSP 1 Score: 144.4 bits (363), Expect = 1.6e-33
Identity = 72/187 (38.50%), Postives = 114/187 (60.96%), Query Frame = 0

Query: 44  FYSYANGSSGLIDPEGIESLCSDMEVNHTDVRILMLAWKMKSEKQGYFNLEEWRTGLKSL 103
           FY YA G   ++ PEG+E  C D+ V   ++ +L+LAWK+++E  G+F  EEW  G+ SL
Sbjct: 55  FYEYA-GPDEVVGPEGMEKFCEDIGVEPENIIMLVLAWKLEAESMGFFTKEEWLKGMTSL 114

Query: 104 RADTVSKLKKALPDLEKEVRRPSNFVDFYSYAFRYCLTEEKQKSIDIESICELLDLVLGS 163
           + D   KL+     L  ++   S+F + Y YAF +   ++ Q+S+DI++   +L L+LG 
Sbjct: 115 QCDCTEKLQNKFDFLRSQLNDISSFKNIYRYAFDFA-RDKDQRSLDIDTAKSMLALLLGR 174

Query: 164 QFHPQVNAFVEYLKIQSDYKVINMDQWMGFFRFCNEISFPELSNYDSELAWPLILDNFVE 223
            + P  + F +YL+ QS Y+V+N DQW     F   +   +LSNYD + AWP++LD FVE
Sbjct: 175 TW-PLFSVFYQYLE-QSKYRVMNKDQWYNVLEFSRTV-HADLSNYDEDGAWPVLLDEFVE 234

Query: 224 WLQAKQS 231
           W + +Q+
Sbjct: 235 WQKVRQT 236

BLAST of Lsi07G004110 vs. ExPASy Swiss-Prot
Match: Q1RMX9 (DCN1-like protein 5 OS=Bos taurus OX=9913 GN=DCUN1D5 PE=2 SV=1)

HSP 1 Score: 144.1 bits (362), Expect = 2.0e-33
Identity = 72/187 (38.50%), Postives = 114/187 (60.96%), Query Frame = 0

Query: 44  FYSYANGSSGLIDPEGIESLCSDMEVNHTDVRILMLAWKMKSEKQGYFNLEEWRTGLKSL 103
           FY YA G   ++ PEG+E  C D+ V   ++ +L+LAWK+++E  G+F  EEW  G+ SL
Sbjct: 54  FYEYA-GPDEVVGPEGMEKFCEDIGVEPENIIMLVLAWKLEAESMGFFTKEEWLKGMTSL 113

Query: 104 RADTVSKLKKALPDLEKEVRRPSNFVDFYSYAFRYCLTEEKQKSIDIESICELLDLVLGS 163
           + D   KL+     L  ++   S+F + Y YAF +   ++ Q+S+DI++   +L L+LG 
Sbjct: 114 QCDCTEKLQNKFDFLRSQLNDISSFKNIYRYAFDFA-RDKDQRSLDIDTAKSMLALLLGR 173

Query: 164 QFHPQVNAFVEYLKIQSDYKVINMDQWMGFFRFCNEISFPELSNYDSELAWPLILDNFVE 223
            + P  + F +YL+ QS Y+V+N DQW     F   +   +LSNYD + AWP++LD FVE
Sbjct: 174 TW-PLFSVFYQYLE-QSKYRVMNKDQWYNVLEFSRTV-HADLSNYDEDGAWPVLLDEFVE 233

Query: 224 WLQAKQS 231
           W + +Q+
Sbjct: 234 WHKVRQA 235

BLAST of Lsi07G004110 vs. ExPASy Swiss-Prot
Match: Q9CXV9 (DCN1-like protein 5 OS=Mus musculus OX=10090 GN=Dcun1d5 PE=1 SV=1)

HSP 1 Score: 144.1 bits (362), Expect = 2.0e-33
Identity = 72/187 (38.50%), Postives = 114/187 (60.96%), Query Frame = 0

Query: 44  FYSYANGSSGLIDPEGIESLCSDMEVNHTDVRILMLAWKMKSEKQGYFNLEEWRTGLKSL 103
           FY YA G   ++ PEG+E  C D+ V   ++ +L+LAWK+++E  G+F  EEW  G+ SL
Sbjct: 55  FYEYA-GPDEVVGPEGMEKFCEDIGVEPENIIMLVLAWKLEAESMGFFTKEEWLKGMTSL 114

Query: 104 RADTVSKLKKALPDLEKEVRRPSNFVDFYSYAFRYCLTEEKQKSIDIESICELLDLVLGS 163
           + D   KL+     L  ++   S+F + Y YAF +   ++ Q+S+DI++   +L L+LG 
Sbjct: 115 QCDCTEKLQSRFDFLRSQLNDISSFKNIYRYAFDFA-RDKDQRSLDIDTAKSMLALLLGR 174

Query: 164 QFHPQVNAFVEYLKIQSDYKVINMDQWMGFFRFCNEISFPELSNYDSELAWPLILDNFVE 223
            + P  + F +YL+ QS Y+V+N DQW     F   +   +LSNYD + AWP++LD FVE
Sbjct: 175 TW-PLFSVFYQYLE-QSKYRVMNKDQWYNVLEFSRTV-HADLSNYDEDGAWPVLLDEFVE 234

Query: 224 WLQAKQS 231
           W + +Q+
Sbjct: 235 WQKIRQT 236

BLAST of Lsi07G004110 vs. ExPASy TrEMBL
Match: A0A1S4DZM9 (Defective in cullin neddylation protein OS=Cucumis melo OX=3656 GN=LOC103494656 PE=4 SV=1)

HSP 1 Score: 444.5 bits (1142), Expect = 2.7e-121
Identity = 218/230 (94.78%), Postives = 228/230 (99.13%), Query Frame = 0

Query: 1   MRRSATRKTGQPNSTSVNSSAVDLFRSASSRASTKELERIDNLFYSYANGSSGLIDPEGI 60
           MRRSATRKTGQPNSTSVNSSAVDLFRSASSRASTKELERID+LFYSYAN +SGLIDPEGI
Sbjct: 1   MRRSATRKTGQPNSTSVNSSAVDLFRSASSRASTKELERIDSLFYSYANPTSGLIDPEGI 60

Query: 61  ESLCSDMEVNHTDVRILMLAWKMKSEKQGYFNLEEWRTGLKSLRADTVSKLKKALPDLEK 120
           E+LCSD+EV+HTDVRILMLAWKMKSEKQGYFNL+EWRTGLKSLRADTVSKLKKALPDLEK
Sbjct: 61  ENLCSDIEVDHTDVRILMLAWKMKSEKQGYFNLDEWRTGLKSLRADTVSKLKKALPDLEK 120

Query: 121 EVRRPSNFVDFYSYAFRYCLTEEKQKSIDIESICELLDLVLGSQFHPQVNAFVEYLKIQS 180
           EVRRPSNFVDFYSYAFRYCLTEEKQKSIDIESICELLDLVLGSQFH QVNAFVEYLKIQ+
Sbjct: 121 EVRRPSNFVDFYSYAFRYCLTEEKQKSIDIESICELLDLVLGSQFHSQVNAFVEYLKIQT 180

Query: 181 DYKVINMDQWMGFFRFCNEISFPELSNYDSELAWPLILDNFVEWLQAKQS 231
           DYKVINMDQWMGFFRFCNEIS+P+LSNYDSELAWPLILDNFVEWLQAKQ+
Sbjct: 181 DYKVINMDQWMGFFRFCNEISYPDLSNYDSELAWPLILDNFVEWLQAKQN 230

BLAST of Lsi07G004110 vs. ExPASy TrEMBL
Match: A0A6J1DKL7 (Defective in cullin neddylation protein OS=Momordica charantia OX=3673 GN=LOC111020935 PE=4 SV=1)

HSP 1 Score: 443.0 bits (1138), Expect = 7.8e-121
Identity = 218/230 (94.78%), Postives = 225/230 (97.83%), Query Frame = 0

Query: 1   MRRSATRKTGQPNSTSVNSSAVDLFRSASSRASTKELERIDNLFYSYANGSSGLIDPEGI 60
           MRRSATRKTGQPNSTSVNSSAVDLFRSASSRASTKELERID+L+YSYAN SSGLIDPEGI
Sbjct: 1   MRRSATRKTGQPNSTSVNSSAVDLFRSASSRASTKELERIDSLYYSYANSSSGLIDPEGI 60

Query: 61  ESLCSDMEVNHTDVRILMLAWKMKSEKQGYFNLEEWRTGLKSLRADTVSKLKKALPDLEK 120
           E+LCSDMEVNHTDVRILMLAWKMKSEKQGYFNLEEWR GLKSLRADTVSKLKKALPDLEK
Sbjct: 61  ETLCSDMEVNHTDVRILMLAWKMKSEKQGYFNLEEWRRGLKSLRADTVSKLKKALPDLEK 120

Query: 121 EVRRPSNFVDFYSYAFRYCLTEEKQKSIDIESICELLDLVLGSQFHPQVNAFVEYLKIQS 180
           EVRRPSNF DFYSYAF+YCLTEEKQKSIDIESICELLDLVLGSQFH QVNAFVEYLK+QS
Sbjct: 121 EVRRPSNFGDFYSYAFQYCLTEEKQKSIDIESICELLDLVLGSQFHSQVNAFVEYLKLQS 180

Query: 181 DYKVINMDQWMGFFRFCNEISFPELSNYDSELAWPLILDNFVEWLQAKQS 231
           DYKVINMDQWMGFFRFCNEIS+P+LSNYDSELAWPLILDNFVEWL AKQS
Sbjct: 181 DYKVINMDQWMGFFRFCNEISYPDLSNYDSELAWPLILDNFVEWLHAKQS 230

BLAST of Lsi07G004110 vs. ExPASy TrEMBL
Match: A0A0A0KYL5 (Defective in cullin neddylation protein OS=Cucumis sativus OX=3659 GN=Csa_4G006480 PE=4 SV=1)

HSP 1 Score: 440.7 bits (1132), Expect = 3.9e-120
Identity = 216/230 (93.91%), Postives = 227/230 (98.70%), Query Frame = 0

Query: 1   MRRSATRKTGQPNSTSVNSSAVDLFRSASSRASTKELERIDNLFYSYANGSSGLIDPEGI 60
           MRRSATRKTGQPNSTSVNSSAVDLFRSASSRAS+KELERID+LFYSYAN SSGLIDPEGI
Sbjct: 92  MRRSATRKTGQPNSTSVNSSAVDLFRSASSRASSKELERIDSLFYSYANPSSGLIDPEGI 151

Query: 61  ESLCSDMEVNHTDVRILMLAWKMKSEKQGYFNLEEWRTGLKSLRADTVSKLKKALPDLEK 120
           E+LCSD+EV+HTDVRILMLAWKMKSEKQGYFNL+EWRTGLKSLRADTVSKLKKALPDLEK
Sbjct: 152 ENLCSDIEVDHTDVRILMLAWKMKSEKQGYFNLDEWRTGLKSLRADTVSKLKKALPDLEK 211

Query: 121 EVRRPSNFVDFYSYAFRYCLTEEKQKSIDIESICELLDLVLGSQFHPQVNAFVEYLKIQS 180
           EVRRPSNFVDFYSYAFRYCLTEEKQKSIDIESICELLDLVLGSQFH QVNAFV+YLKIQ+
Sbjct: 212 EVRRPSNFVDFYSYAFRYCLTEEKQKSIDIESICELLDLVLGSQFHSQVNAFVDYLKIQT 271

Query: 181 DYKVINMDQWMGFFRFCNEISFPELSNYDSELAWPLILDNFVEWLQAKQS 231
           DYKVINMDQWMGFFRFCNEIS+P+L NYDSELAWPLILDNFVEWLQAKQ+
Sbjct: 272 DYKVINMDQWMGFFRFCNEISYPDLINYDSELAWPLILDNFVEWLQAKQN 321

BLAST of Lsi07G004110 vs. ExPASy TrEMBL
Match: A0A6J1GVA7 (Defective in cullin neddylation protein OS=Cucurbita moschata OX=3662 GN=LOC111457515 PE=4 SV=1)

HSP 1 Score: 438.7 bits (1127), Expect = 1.5e-119
Identity = 217/230 (94.35%), Postives = 225/230 (97.83%), Query Frame = 0

Query: 1   MRRSATRKTGQPNSTSVNSSAVDLFRSASSRASTKELERIDNLFYSYANGSSGLIDPEGI 60
           MRRSAT+KTGQPNS SVNSSAVDLFRSASSRAS+KELERID+LFYSYANGSSGLIDPEGI
Sbjct: 1   MRRSATKKTGQPNSASVNSSAVDLFRSASSRASSKELERIDSLFYSYANGSSGLIDPEGI 60

Query: 61  ESLCSDMEVNHTDVRILMLAWKMKSEKQGYFNLEEWRTGLKSLRADTVSKLKKALPDLEK 120
           ESLCSDMEVN TDVRILMLAWKMKSEKQGYF LEEWRTGLKSLRADTVSKLKKALPDLEK
Sbjct: 61  ESLCSDMEVNPTDVRILMLAWKMKSEKQGYFTLEEWRTGLKSLRADTVSKLKKALPDLEK 120

Query: 121 EVRRPSNFVDFYSYAFRYCLTEEKQKSIDIESICELLDLVLGSQFHPQVNAFVEYLKIQS 180
           EVRR SNFVDFYSYAFRYCLTE+KQKSIDIESICELLDLVLGS+FH QV+AF+EYLKIQS
Sbjct: 121 EVRRLSNFVDFYSYAFRYCLTEDKQKSIDIESICELLDLVLGSEFHAQVHAFIEYLKIQS 180

Query: 181 DYKVINMDQWMGFFRFCNEISFPELSNYDSELAWPLILDNFVEWLQAKQS 231
           DYKVINMDQWMGFFRFCNEISFP+LSNYDSELAWPLILDNFVEWLQAKQS
Sbjct: 181 DYKVINMDQWMGFFRFCNEISFPDLSNYDSELAWPLILDNFVEWLQAKQS 230

BLAST of Lsi07G004110 vs. ExPASy TrEMBL
Match: A0A6J1J092 (Defective in cullin neddylation protein OS=Cucurbita maxima OX=3661 GN=LOC111480062 PE=4 SV=1)

HSP 1 Score: 436.4 bits (1121), Expect = 7.3e-119
Identity = 217/230 (94.35%), Postives = 223/230 (96.96%), Query Frame = 0

Query: 1   MRRSATRKTGQPNSTSVNSSAVDLFRSASSRASTKELERIDNLFYSYANGSSGLIDPEGI 60
           MRRSATRKTGQPNS SVNSSAVDLFR ASSRAS+KELERID+LFYSYANGSSGLIDPEGI
Sbjct: 1   MRRSATRKTGQPNSASVNSSAVDLFRYASSRASSKELERIDSLFYSYANGSSGLIDPEGI 60

Query: 61  ESLCSDMEVNHTDVRILMLAWKMKSEKQGYFNLEEWRTGLKSLRADTVSKLKKALPDLEK 120
           ESLCSDMEVN TDVRILMLAWKMKSEKQGYF LEEWRTGLKSLRADTVSKLKKALPDLEK
Sbjct: 61  ESLCSDMEVNPTDVRILMLAWKMKSEKQGYFTLEEWRTGLKSLRADTVSKLKKALPDLEK 120

Query: 121 EVRRPSNFVDFYSYAFRYCLTEEKQKSIDIESICELLDLVLGSQFHPQVNAFVEYLKIQS 180
           EVRR SNFVDFYSYAFRYCLTE+KQKSIDIESICELLDLVLGS+FH QV AF+EYLKIQS
Sbjct: 121 EVRRLSNFVDFYSYAFRYCLTEDKQKSIDIESICELLDLVLGSEFHAQVYAFIEYLKIQS 180

Query: 181 DYKVINMDQWMGFFRFCNEISFPELSNYDSELAWPLILDNFVEWLQAKQS 231
           DYKVINMDQWMGFFRFCNEISFP+LSNYDSELAWPLILDNFVEWLQAKQS
Sbjct: 181 DYKVINMDQWMGFFRFCNEISFPDLSNYDSELAWPLILDNFVEWLQAKQS 230

BLAST of Lsi07G004110 vs. NCBI nr
Match: XP_038876378.1 (DCN1-like protein 4 [Benincasa hispida] >XP_038876439.1 DCN1-like protein 4 [Benincasa hispida])

HSP 1 Score: 451.1 bits (1159), Expect = 5.9e-123
Identity = 223/230 (96.96%), Postives = 228/230 (99.13%), Query Frame = 0

Query: 1   MRRSATRKTGQPNSTSVNSSAVDLFRSASSRASTKELERIDNLFYSYANGSSGLIDPEGI 60
           MRRSATRKTGQPNSTSVNSSAVDLFRSASSRAS+KELERID+LFYSYAN SSGLIDPEGI
Sbjct: 1   MRRSATRKTGQPNSTSVNSSAVDLFRSASSRASSKELERIDSLFYSYANASSGLIDPEGI 60

Query: 61  ESLCSDMEVNHTDVRILMLAWKMKSEKQGYFNLEEWRTGLKSLRADTVSKLKKALPDLEK 120
           ESLCSDMEVNHTDVRILMLAWKMKSEKQGYFNLEEWRTGLKSLRADTVSKLKKALPDLEK
Sbjct: 61  ESLCSDMEVNHTDVRILMLAWKMKSEKQGYFNLEEWRTGLKSLRADTVSKLKKALPDLEK 120

Query: 121 EVRRPSNFVDFYSYAFRYCLTEEKQKSIDIESICELLDLVLGSQFHPQVNAFVEYLKIQS 180
           EVRRPSNFVDFYSYAFRYCLTEEKQKSIDIESICELLDLVLGSQFHPQV+AFVEYLKIQS
Sbjct: 121 EVRRPSNFVDFYSYAFRYCLTEEKQKSIDIESICELLDLVLGSQFHPQVDAFVEYLKIQS 180

Query: 181 DYKVINMDQWMGFFRFCNEISFPELSNYDSELAWPLILDNFVEWLQAKQS 231
           DYKVINMDQWMGFFRFCNEISF +LSNYDSELAWPLILDNFVEWLQAKQ+
Sbjct: 181 DYKVINMDQWMGFFRFCNEISFSDLSNYDSELAWPLILDNFVEWLQAKQN 230

BLAST of Lsi07G004110 vs. NCBI nr
Match: XP_008454171.1 (PREDICTED: DCN1-like protein 4 [Cucumis melo] >XP_016901452.1 PREDICTED: DCN1-like protein 4 [Cucumis melo])

HSP 1 Score: 444.5 bits (1142), Expect = 5.5e-121
Identity = 218/230 (94.78%), Postives = 228/230 (99.13%), Query Frame = 0

Query: 1   MRRSATRKTGQPNSTSVNSSAVDLFRSASSRASTKELERIDNLFYSYANGSSGLIDPEGI 60
           MRRSATRKTGQPNSTSVNSSAVDLFRSASSRASTKELERID+LFYSYAN +SGLIDPEGI
Sbjct: 1   MRRSATRKTGQPNSTSVNSSAVDLFRSASSRASTKELERIDSLFYSYANPTSGLIDPEGI 60

Query: 61  ESLCSDMEVNHTDVRILMLAWKMKSEKQGYFNLEEWRTGLKSLRADTVSKLKKALPDLEK 120
           E+LCSD+EV+HTDVRILMLAWKMKSEKQGYFNL+EWRTGLKSLRADTVSKLKKALPDLEK
Sbjct: 61  ENLCSDIEVDHTDVRILMLAWKMKSEKQGYFNLDEWRTGLKSLRADTVSKLKKALPDLEK 120

Query: 121 EVRRPSNFVDFYSYAFRYCLTEEKQKSIDIESICELLDLVLGSQFHPQVNAFVEYLKIQS 180
           EVRRPSNFVDFYSYAFRYCLTEEKQKSIDIESICELLDLVLGSQFH QVNAFVEYLKIQ+
Sbjct: 121 EVRRPSNFVDFYSYAFRYCLTEEKQKSIDIESICELLDLVLGSQFHSQVNAFVEYLKIQT 180

Query: 181 DYKVINMDQWMGFFRFCNEISFPELSNYDSELAWPLILDNFVEWLQAKQS 231
           DYKVINMDQWMGFFRFCNEIS+P+LSNYDSELAWPLILDNFVEWLQAKQ+
Sbjct: 181 DYKVINMDQWMGFFRFCNEISYPDLSNYDSELAWPLILDNFVEWLQAKQN 230

BLAST of Lsi07G004110 vs. NCBI nr
Match: XP_022153426.1 (DCN1-like protein 4 [Momordica charantia])

HSP 1 Score: 443.0 bits (1138), Expect = 1.6e-120
Identity = 218/230 (94.78%), Postives = 225/230 (97.83%), Query Frame = 0

Query: 1   MRRSATRKTGQPNSTSVNSSAVDLFRSASSRASTKELERIDNLFYSYANGSSGLIDPEGI 60
           MRRSATRKTGQPNSTSVNSSAVDLFRSASSRASTKELERID+L+YSYAN SSGLIDPEGI
Sbjct: 1   MRRSATRKTGQPNSTSVNSSAVDLFRSASSRASTKELERIDSLYYSYANSSSGLIDPEGI 60

Query: 61  ESLCSDMEVNHTDVRILMLAWKMKSEKQGYFNLEEWRTGLKSLRADTVSKLKKALPDLEK 120
           E+LCSDMEVNHTDVRILMLAWKMKSEKQGYFNLEEWR GLKSLRADTVSKLKKALPDLEK
Sbjct: 61  ETLCSDMEVNHTDVRILMLAWKMKSEKQGYFNLEEWRRGLKSLRADTVSKLKKALPDLEK 120

Query: 121 EVRRPSNFVDFYSYAFRYCLTEEKQKSIDIESICELLDLVLGSQFHPQVNAFVEYLKIQS 180
           EVRRPSNF DFYSYAF+YCLTEEKQKSIDIESICELLDLVLGSQFH QVNAFVEYLK+QS
Sbjct: 121 EVRRPSNFGDFYSYAFQYCLTEEKQKSIDIESICELLDLVLGSQFHSQVNAFVEYLKLQS 180

Query: 181 DYKVINMDQWMGFFRFCNEISFPELSNYDSELAWPLILDNFVEWLQAKQS 231
           DYKVINMDQWMGFFRFCNEIS+P+LSNYDSELAWPLILDNFVEWL AKQS
Sbjct: 181 DYKVINMDQWMGFFRFCNEISYPDLSNYDSELAWPLILDNFVEWLHAKQS 230

BLAST of Lsi07G004110 vs. NCBI nr
Match: XP_004152174.1 (DCN1-like protein 5 [Cucumis sativus] >KAE8649068.1 hypothetical protein Csa_014584 [Cucumis sativus])

HSP 1 Score: 440.7 bits (1132), Expect = 8.0e-120
Identity = 216/230 (93.91%), Postives = 227/230 (98.70%), Query Frame = 0

Query: 1   MRRSATRKTGQPNSTSVNSSAVDLFRSASSRASTKELERIDNLFYSYANGSSGLIDPEGI 60
           MRRSATRKTGQPNSTSVNSSAVDLFRSASSRAS+KELERID+LFYSYAN SSGLIDPEGI
Sbjct: 1   MRRSATRKTGQPNSTSVNSSAVDLFRSASSRASSKELERIDSLFYSYANPSSGLIDPEGI 60

Query: 61  ESLCSDMEVNHTDVRILMLAWKMKSEKQGYFNLEEWRTGLKSLRADTVSKLKKALPDLEK 120
           E+LCSD+EV+HTDVRILMLAWKMKSEKQGYFNL+EWRTGLKSLRADTVSKLKKALPDLEK
Sbjct: 61  ENLCSDIEVDHTDVRILMLAWKMKSEKQGYFNLDEWRTGLKSLRADTVSKLKKALPDLEK 120

Query: 121 EVRRPSNFVDFYSYAFRYCLTEEKQKSIDIESICELLDLVLGSQFHPQVNAFVEYLKIQS 180
           EVRRPSNFVDFYSYAFRYCLTEEKQKSIDIESICELLDLVLGSQFH QVNAFV+YLKIQ+
Sbjct: 121 EVRRPSNFVDFYSYAFRYCLTEEKQKSIDIESICELLDLVLGSQFHSQVNAFVDYLKIQT 180

Query: 181 DYKVINMDQWMGFFRFCNEISFPELSNYDSELAWPLILDNFVEWLQAKQS 231
           DYKVINMDQWMGFFRFCNEIS+P+L NYDSELAWPLILDNFVEWLQAKQ+
Sbjct: 181 DYKVINMDQWMGFFRFCNEISYPDLINYDSELAWPLILDNFVEWLQAKQN 230

BLAST of Lsi07G004110 vs. NCBI nr
Match: KAG6581963.1 (DCN1-like protein 4, partial [Cucurbita argyrosperma subsp. sororia] >KAG7018382.1 DCN1-like protein 4 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 439.9 bits (1130), Expect = 1.4e-119
Identity = 218/230 (94.78%), Postives = 225/230 (97.83%), Query Frame = 0

Query: 1   MRRSATRKTGQPNSTSVNSSAVDLFRSASSRASTKELERIDNLFYSYANGSSGLIDPEGI 60
           MRRSATRKTGQPNS SVNSSAVDLFRSASSRAS+KELERID+LFYSYANGSSGLIDPEGI
Sbjct: 1   MRRSATRKTGQPNSASVNSSAVDLFRSASSRASSKELERIDSLFYSYANGSSGLIDPEGI 60

Query: 61  ESLCSDMEVNHTDVRILMLAWKMKSEKQGYFNLEEWRTGLKSLRADTVSKLKKALPDLEK 120
           ESLCSDMEVN TDVRILMLAWKMKSEKQGYF LEEWRTGLKSLRADTVSKLKKALPDLEK
Sbjct: 61  ESLCSDMEVNPTDVRILMLAWKMKSEKQGYFTLEEWRTGLKSLRADTVSKLKKALPDLEK 120

Query: 121 EVRRPSNFVDFYSYAFRYCLTEEKQKSIDIESICELLDLVLGSQFHPQVNAFVEYLKIQS 180
           EVRR SNFVDFYSYAFRYCLTE+KQKSIDIESICELLDLVLGS+FH QV+AF+EYLKIQS
Sbjct: 121 EVRRLSNFVDFYSYAFRYCLTEDKQKSIDIESICELLDLVLGSEFHAQVHAFIEYLKIQS 180

Query: 181 DYKVINMDQWMGFFRFCNEISFPELSNYDSELAWPLILDNFVEWLQAKQS 231
           DYKVINMDQWMGFFRFCNEISFP+LSNYDSELAWPLILDNFVEWLQAKQS
Sbjct: 181 DYKVINMDQWMGFFRFCNEISFPDLSNYDSELAWPLILDNFVEWLQAKQS 230

BLAST of Lsi07G004110 vs. TAIR 10
Match: AT1G15860.1 (Domain of unknown function (DUF298) )

HSP 1 Score: 352.8 bits (904), Expect = 2.0e-97
Identity = 168/230 (73.04%), Postives = 202/230 (87.83%), Query Frame = 0

Query: 1   MRRSATRKTGQPNSTSVNSSAVDLFRSASSRASTKELERIDNLFYSYANGSSGLIDPEGI 60
           MRRS+++K    ++ SV +   DLFRSASS+AS KE++RID+LF  YAN SS LIDPEGI
Sbjct: 1   MRRSSSKKKSGQSTESVTT---DLFRSASSKASNKEMDRIDHLFNQYANKSSSLIDPEGI 60

Query: 61  ESLCSDMEVNHTDVRILMLAWKMKSEKQGYFNLEEWRTGLKSLRADTVSKLKKALPDLEK 120
           E LCS++EV+HTD+RILMLAWKMK+EKQGYF  EEWR GLK+LRADT++KLKKALP+LEK
Sbjct: 61  EELCSNLEVSHTDIRILMLAWKMKAEKQGYFTHEEWRRGLKALRADTINKLKKALPELEK 120

Query: 121 EVRRPSNFVDFYSYAFRYCLTEEKQKSIDIESICELLDLVLGSQFHPQVNAFVEYLKIQS 180
           EVRRPSNF DFY+YAF YCLTEEKQKSIDIE+IC+LL++V+GS F  QV+ FVEYLKIQ+
Sbjct: 121 EVRRPSNFADFYAYAFCYCLTEEKQKSIDIETICQLLEIVMGSTFRAQVDYFVEYLKIQN 180

Query: 181 DYKVINMDQWMGFFRFCNEISFPELSNYDSELAWPLILDNFVEWLQAKQS 231
           DYKVINMDQWMG +RFCNEISFP++ +Y+ ELAWPLILDNFVEW+Q KQ+
Sbjct: 181 DYKVINMDQWMGLYRFCNEISFPDMGDYNPELAWPLILDNFVEWIQEKQA 227

BLAST of Lsi07G004110 vs. TAIR 10
Match: AT1G15860.2 (Domain of unknown function (DUF298) )

HSP 1 Score: 352.8 bits (904), Expect = 2.0e-97
Identity = 168/230 (73.04%), Postives = 202/230 (87.83%), Query Frame = 0

Query: 1   MRRSATRKTGQPNSTSVNSSAVDLFRSASSRASTKELERIDNLFYSYANGSSGLIDPEGI 60
           MRRS+++K    ++ SV +   DLFRSASS+AS KE++RID+LF  YAN SS LIDPEGI
Sbjct: 1   MRRSSSKKKSGQSTESVTT---DLFRSASSKASNKEMDRIDHLFNQYANKSSSLIDPEGI 60

Query: 61  ESLCSDMEVNHTDVRILMLAWKMKSEKQGYFNLEEWRTGLKSLRADTVSKLKKALPDLEK 120
           E LCS++EV+HTD+RILMLAWKMK+EKQGYF  EEWR GLK+LRADT++KLKKALP+LEK
Sbjct: 61  EELCSNLEVSHTDIRILMLAWKMKAEKQGYFTHEEWRRGLKALRADTINKLKKALPELEK 120

Query: 121 EVRRPSNFVDFYSYAFRYCLTEEKQKSIDIESICELLDLVLGSQFHPQVNAFVEYLKIQS 180
           EVRRPSNF DFY+YAF YCLTEEKQKSIDIE+IC+LL++V+GS F  QV+ FVEYLKIQ+
Sbjct: 121 EVRRPSNFADFYAYAFCYCLTEEKQKSIDIETICQLLEIVMGSTFRAQVDYFVEYLKIQN 180

Query: 181 DYKVINMDQWMGFFRFCNEISFPELSNYDSELAWPLILDNFVEWLQAKQS 231
           DYKVINMDQWMG +RFCNEISFP++ +Y+ ELAWPLILDNFVEW+Q KQ+
Sbjct: 181 DYKVINMDQWMGLYRFCNEISFPDMGDYNPELAWPLILDNFVEWIQEKQA 227

BLAST of Lsi07G004110 vs. TAIR 10
Match: AT1G15860.3 (Domain of unknown function (DUF298) )

HSP 1 Score: 345.1 bits (884), Expect = 4.3e-95
Identity = 168/239 (70.29%), Postives = 202/239 (84.52%), Query Frame = 0

Query: 1   MRRSATRKTGQPNSTSVNSSAVDLFRSASSRASTKELERIDNLFYSYANGSSGLIDPEGI 60
           MRRS+++K    ++ SV +   DLFRSASS+AS KE++RID+LF  YAN SS LIDPEGI
Sbjct: 1   MRRSSSKKKSGQSTESVTT---DLFRSASSKASNKEMDRIDHLFNQYANKSSSLIDPEGI 60

Query: 61  ESLCSDMEVNHTDVRILMLAWKMKSEKQGYFNLEEWRTGLKSLRADTVSKLKKALPDLEK 120
           E LCS++EV+HTD+RILMLAWKMK+EKQGYF  EEWR GLK+LRADT++KLKKALP+LEK
Sbjct: 61  EELCSNLEVSHTDIRILMLAWKMKAEKQGYFTHEEWRRGLKALRADTINKLKKALPELEK 120

Query: 121 EVRRPSNFVDFYSYAFRYCLTEEKQKSIDIESICELLDLVLGSQFHPQVNAFVEYLK--- 180
           EVRRPSNF DFY+YAF YCLTEEKQKSIDIE+IC+LL++V+GS F  QV+ FVEYLK   
Sbjct: 121 EVRRPSNFADFYAYAFCYCLTEEKQKSIDIETICQLLEIVMGSTFRAQVDYFVEYLKVWI 180

Query: 181 ------IQSDYKVINMDQWMGFFRFCNEISFPELSNYDSELAWPLILDNFVEWLQAKQS 231
                 IQ+DYKVINMDQWMG +RFCNEISFP++ +Y+ ELAWPLILDNFVEW+Q KQ+
Sbjct: 181 TQKSHIIQNDYKVINMDQWMGLYRFCNEISFPDMGDYNPELAWPLILDNFVEWIQEKQA 236

BLAST of Lsi07G004110 vs. TAIR 10
Match: AT3G12760.1 (CONTAINS InterPro DOMAIN/s: Defective-in-cullin neddylation protein (InterPro:IPR014764), Protein of unknown function DUF298 (InterPro:IPR005176), UBA-like (InterPro:IPR009060); BEST Arabidopsis thaliana protein match is: Domain of unknown function (DUF298) (TAIR:AT1G15860.2); Has 857 Blast hits to 855 proteins in 202 species: Archae - 0; Bacteria - 0; Metazoa - 482; Fungi - 154; Plants - 139; Viruses - 0; Other Eukaryotes - 82 (source: NCBI BLink). )

HSP 1 Score: 123.2 bits (308), Expect = 2.6e-28
Identity = 68/210 (32.38%), Postives = 121/210 (57.62%), Query Frame = 0

Query: 20  SAVDLFRS-ASSRASTKELERIDNLFYSYANGSSGLIDPEGIESLCSDMEVNHTDVRILM 79
           +A D+F S    R++  E+ R++ L+  Y +  S +I  EGI  LC+D+EV   D+  L+
Sbjct: 39  AAFDVFYSQPQPRSNAAEVRRLEELYNRYKDPYSDMILAEGISVLCNDLEVEPQDIVTLV 98

Query: 80  LAWKMKSEKQGYFNLEEWRTGLKSLRADTVSKLKKALPDLEKEVRRPSNFVDFYSYAFRY 139
           L+W M +     F+ +E+ +GL++L  D++ KL++ LP +  E++    F + Y++AF +
Sbjct: 99  LSWHMNAATACEFSRQEFISGLQALGVDSIGKLQEKLPFMRSELKDEQKFHEIYNFAFGW 158

Query: 140 CLTEEKQKSIDIESICELLDLVLGSQFHPQVNAFVEYLKIQSDYKVINMDQWMGFFRFCN 199
              E+ QKS+ +++   +  L+   +  P V  + ++L+ + + K I+ D W     F  
Sbjct: 159 A-KEKGQKSLALDTAIGMWQLLFAEREWPLVTHWCDFLQDRHN-KAISKDTWAQLLEFSR 218

Query: 200 EISFPELSNYDSELAWPLILDNFVEWLQAK 229
            +  P LSNYD+E AWP ++D FVE+L  K
Sbjct: 219 MVD-PVLSNYDAEGAWPYLIDEFVEYLYDK 245

BLAST of Lsi07G004110 vs. TAIR 10
Match: AT3G28970.1 (Domain of unknown function (DUF298) )

HSP 1 Score: 49.7 bits (117), Expect = 3.7e-06
Identity = 31/98 (31.63%), Postives = 48/98 (48.98%), Query Frame = 0

Query: 126 SNFVDFYSYAFRYCLTEEKQKSIDIESICELLDLVLGSQFHPQVNAFVEYLKIQSDYKVI 185
           + F  FY + F  C  E  QK+I I        LVL  +F   +N + ++++    +  I
Sbjct: 84  TEFTCFYDFVFFMC-RENGQKNITISRAITAWKLVLAGRFR-LLNRWCDFIEKNQRHN-I 143

Query: 186 NMDQWMGFFRFCNEISFPELSNYDSELAWPLILDNFVE 224
           + D W     F +      L  YDSE AWP+++D+FVE
Sbjct: 144 SEDTWQQVLAF-SRCVHENLEGYDSEGAWPVLIDDFVE 177

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q925648.2e-3540.98DCN1-like protein 4 OS=Homo sapiens OX=9606 GN=DCUN1D4 PE=1 SV=2[more]
Q8CCA08.2e-3541.46DCN1-like protein 4 OS=Mus musculus OX=10090 GN=Dcun1d4 PE=2 SV=1[more]
Q9BTE71.6e-3338.50DCN1-like protein 5 OS=Homo sapiens OX=9606 GN=DCUN1D5 PE=1 SV=1[more]
Q1RMX92.0e-3338.50DCN1-like protein 5 OS=Bos taurus OX=9913 GN=DCUN1D5 PE=2 SV=1[more]
Q9CXV92.0e-3338.50DCN1-like protein 5 OS=Mus musculus OX=10090 GN=Dcun1d5 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A1S4DZM92.7e-12194.78Defective in cullin neddylation protein OS=Cucumis melo OX=3656 GN=LOC103494656 ... [more]
A0A6J1DKL77.8e-12194.78Defective in cullin neddylation protein OS=Momordica charantia OX=3673 GN=LOC111... [more]
A0A0A0KYL53.9e-12093.91Defective in cullin neddylation protein OS=Cucumis sativus OX=3659 GN=Csa_4G0064... [more]
A0A6J1GVA71.5e-11994.35Defective in cullin neddylation protein OS=Cucurbita moschata OX=3662 GN=LOC1114... [more]
A0A6J1J0927.3e-11994.35Defective in cullin neddylation protein OS=Cucurbita maxima OX=3661 GN=LOC111480... [more]
Match NameE-valueIdentityDescription
XP_038876378.15.9e-12396.96DCN1-like protein 4 [Benincasa hispida] >XP_038876439.1 DCN1-like protein 4 [Ben... [more]
XP_008454171.15.5e-12194.78PREDICTED: DCN1-like protein 4 [Cucumis melo] >XP_016901452.1 PREDICTED: DCN1-li... [more]
XP_022153426.11.6e-12094.78DCN1-like protein 4 [Momordica charantia][more]
XP_004152174.18.0e-12093.91DCN1-like protein 5 [Cucumis sativus] >KAE8649068.1 hypothetical protein Csa_014... [more]
KAG6581963.11.4e-11994.78DCN1-like protein 4, partial [Cucurbita argyrosperma subsp. sororia] >KAG7018382... [more]
Match NameE-valueIdentityDescription
AT1G15860.12.0e-9773.04Domain of unknown function (DUF298) [more]
AT1G15860.22.0e-9773.04Domain of unknown function (DUF298) [more]
AT1G15860.34.3e-9570.29Domain of unknown function (DUF298) [more]
AT3G12760.12.6e-2832.38CONTAINS InterPro DOMAIN/s: Defective-in-cullin neddylation protein (InterPro:IP... [more]
AT3G28970.13.7e-0631.63Domain of unknown function (DUF298) [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR005176Potentiating neddylation domainPFAMPF03556Cullin_bindingcoord: 110..224
e-value: 3.6E-27
score: 95.2
IPR005176Potentiating neddylation domainPROSITEPS51229DCUN1coord: 35..226
score: 42.622265
IPR042460DCN1-like, PONY binding domainGENE3D1.10.238.200Cullin, PONY binding domaincoord: 125..230
e-value: 1.2E-28
score: 101.4
NoneNo IPR availableGENE3D1.10.238.10coord: 33..123
e-value: 4.1E-28
score: 99.2
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..20
NoneNo IPR availablePANTHERPTHR12281:SF12DEFECTIVE IN CULLIN NEDDYLATION PROTEINcoord: 1..229
IPR014764Defective-in-cullin neddylation proteinPANTHERPTHR12281RP42 RELATEDcoord: 1..229
IPR011992EF-hand domain pairSUPERFAMILY47473EF-handcoord: 34..197

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi07G004110.1Lsi07G004110.1mRNA