Lsi07G002590 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi07G002590
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionLaccase
Locationchr07: 2837465 .. 2840176 (+)
RNA-Seq ExpressionLsi07G002590
SyntenyLsi07G002590
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTTAGTAATCTAGTTATATTTTCCCACAAAAAACAAAAAAAGGTACTTCTCAAAGGCAAATTCTTCGTACAACACTGTAACATTGAAATATATGAATTGAACATAAGTTCAAGTGAACATCAAGTCTAACATTTCTCTTTCATTTGGAAATCCCAGCCTCCAAGTTAGTCAAAAGCAAGGCAAGTAGTCTCTATCAGTATTTTGCTTGCAAGCTTTGGCTATGGAGACCCCCTTGCACAATTCCTACCAACTTGTTCTCACAAACCTTCAAATCACTGCAGCTTTCTAAATGGTATAAATACTTGGCATTGTTCACATTTTAAATCATTCATACTTGCATTACCTCAAAAAAAACCATTGCCAAAAGACAATGGCCAGCTTGGTTTGGTTCGTTCATCGTCTGACATTGCTCTTCGTTGCCTGTTGTTTAGGATTAATCTCATTTTCTGCAGAAGCTGCAATAAAGAGATATCAGTTTGATGTGAGTTATATAAGTTATTACATGGATTCAATAGCTGTAGATGTAATATATGTAGACAAGGATATTGAATCTAGATCGATCAGTTTTATTTAACTTACCTTTCTTCTGAGATTTTGTTTCTCAGGTTCAAGTGCAGAATATAAGTAGATTGTGTCATGCAAAGCCAATTGTCACCGTAAACGGGAAGTTTCCGGGGCCGACGATATATGTACAAGAAGGAGATCGAGTTCTTGTCAATGTTACAAACCATGCTCAATATAACATGTCGATTCACTGGTAATAATGAGAAAGGACCTTAATGACACGAGTGAAAATTGAGCCACCCCCCACCCCCCAATATATGTGTGTATTTATATATGTTACTTGGGTTCTTATCTCAGTCGCCTTCTGCATATTCTGCAGGCATGGATTGAAGCAGTATCGAAACGGCTGGGCAGATGGACCTGCTTACATCACACAATGTCCTATTCAGAGAGGAAACAGCTATACCTATGATTTCAATGTCACAGGACAAAGAGGCACATTATGGTGGCATGCACACATCCTTTGGCTAAGGGCAACAGTTTATGGAGCAATTGTCATAATGCCCAAGCAAGGAACCCCTTATCCATTCCCTCAACCAAATAGAGAATTTGAAATTCTTCTGGGTTAGTTGAGAAAATTTACTAGAAATTATAGTGTTTGGATCATTGCAAGAAAATGAAAATTATGACGGAGTTGATACCTCTGGTGCAGGAGAATGGTGGAATAATGATGTTGAGGCGGTTGTCAACCAAGGATCCAGCATGGGCGTGCCACCAAATATGTCTGATGCTCACACAATCAATGGCAAGTCAGGGCCGCTCTTCCCTTGTTCGGAAAAACGTAAGCAATATTAATTGAAGAAAGCTTTTGGGTCTTTGACACCGGTTTCAATGAACAGACCACTGATAATGATTTGGTTTTCCTTTGTGCTTCATTCTTAGATACTTTCGCCATGGAGGTTGAAACTGGAAAGACATATCTCTTGAGAATAATAAATGCTGCCCTCAACGATGAGCTTTTCTTTGCCATTGCTGGTCACAACTTGACGGTCGTAGAGGTTGATGCAGTTTACACAAAATCATTCAGTACACAAGCTATACTAATTGCACCAGGACAGACCACAAATGTCTTGTTGCAAGCCAACCAAGCACCCAATAGATACTTCATGGCTTCCAGGTCGTTCATGGACGTTCCAATTCCTGTGGACAACAAAACAGCCACCGGCATTCTCCAATACAAAGGAATCCCTAACACAATCCTCCCAATCCTTCCCCAATTACCGGCCTCAAATGACACGTCATTTGCTTTAAACTACAACAAGAAGCTTAAAAGCTTAAACTCACCTCAATATCCTGCTAATGTTCCACTCAAAGTTGATCGAAAGCTTTTCTACACAATTGGTTTGGGCCAAAACGCTTGCCCTACTTGTCTAAATGGAACACAATTCGTTGCTTCATTAAACAATATCTCATTTGTGATGCCCCAAATTGGTCTTCTTCAATCACATTACTTCAATATCAGTGGGGTATTTAGAACTGATTTCCCAGACAGACCTCCAACTCCATTCAACTATACTGGAGCACCACTGACCGCTAATCTGAGAACTGCAGTGGGCACGAGACTTAGCAAGATTGCATTCAATTCTACGGTGGAGTTGGTACTACAGGACACCAATCTTCTTACTGTCGAGTCCCATCCATTCCATCTCCATGGCTACAACTTTTTCGTTGTTGGAACGGGGATCGGAAACTTTAATCCAGCCAAAGATCCAGCAAAATACAACTTGGTCGATCCTGTCGAGAGAAACACAGTTGGAGTTCCCACTGGAGGATGGACTGCCATCCGATTTAGAGCCGATAACCCAGGTGGATATCGATATTATTGACTTTAAAATATGAACAAAGTTGAAATATGACTTATAGTAAATATATATATATATATATAGCTCATATCATTTTGGTAATGAACGTACAGGAGTCTGGTTCATGCATTGTCATCTTGAGGTTCATACAGGATGGGGTCTGAAAACAGCATTTGTGGTTGAAGATGGACCTGGAAAAGATCAAACCATTCTTCCACCCCCAAAGGATCTTCCACCATGCTAGCCTTTGTACCTGCCAAAACATAAACAAAGTGTTCTTTTGATTCCTGCTCAAGTTGCAGGAAATTTCTTTGCGACTTAAAATTACACTAAGATGCCAAAAA

mRNA sequence

ATGTTAACAATGGCCAGCTTGGTTTGGTTCGTTCATCGTCTGACATTGCTCTTCGTTGCCTGTTGTTTAGGATTAATCTCATTTTCTGCAGAAGCTGCAATAAAGAGATATCAGTTTGATGTTCAAGTGCAGAATATAAGTAGATTGTGTCATGCAAAGCCAATTGTCACCGTAAACGGGAAGTTTCCGGGGCCGACGATATATGTACAAGAAGGAGATCGAGTTCTTGTCAATGTTACAAACCATGCTCAATATAACATGACCTTAATGACACGAGTGAAAATTGAGCCACCCCCCACCCCCCAATATATGTGTGTATTTATATATGTTACTTGGGTTCTTATCTCAGTCGCCTTCTGCATATTCTGCAGGCATGGATTGAAGCAGTATCGAAACGGCTGGGCAGATGGACCTGCTTACATCACACAATGTCCTATTCAGAGAGGAAACAGCTATACCTATGATTTCAATGTCACAGGACAAAGAGGCACATTATGGTGGCATGCACACATCCTTTGGCTAAGGGCAACAGTTTATGGAGCAATTGTCATAATGCCCAAGCAAGGAACCCCTTATCCATTCCCTCAACCAAATAGAGAATTTGAAATTCTTCTGGGAGAATGGTGGAATAATGATGTTGAGGCGGTTGTCAACCAAGGATCCAGCATGGGCGTGCCACCAAATATGTCTGATGCTCACACAATCAATGGCAAGTCAGGGCCGCTCTTCCCTTGTTCGGAAAAACATACTTTCGCCATGGAGGTTGAAACTGGAAAGACATATCTCTTGAGAATAATAAATGCTGCCCTCAACGATGAGCTTTTCTTTGCCATTGCTGGTCACAACTTGACGGTCGTAGAGGTTGATGCAGTTTACACAAAATCATTCAGTACACAAGCTATACTAATTGCACCAGGACAGACCACAAATGTCTTGTTGCAAGCCAACCAAGCACCCAATAGATACTTCATGGCTTCCAGGTCGTTCATGGACGTTCCAATTCCTGTGGACAACAAAACAGCCACCGGCATTCTCCAATACAAAGGAATCCCTAACACAATCCTCCCAATCCTTCCCCAATTACCGGCCTCAAATGACACGTCATTTGCTTTAAACTACAACAAGAAGCTTAAAAGCTTAAACTCACCTCAATATCCTGCTAATGTTCCACTCAAAGTTGATCGAAAGCTTTTCTACACAATTGGTTTGGGCCAAAACGCTTGCCCTACTTGTCTAAATGGAACACAATTCGTTGCTTCATTAAACAATATCTCATTTGTGATGCCCCAAATTGGTCTTCTTCAATCACATTACTTCAATATCAGTGGGGTATTTAGAACTGATTTCCCAGACAGACCTCCAACTCCATTCAACTATACTGGAGCACCACTGACCGCTAATCTGAGAACTGCAGTGGGCACGAGACTTAGCAAGATTGCATTCAATTCTACGGTGGAGTTGGTACTACAGGACACCAATCTTCTTACTGTCGAGTCCCATCCATTCCATCTCCATGGCTACAACTTTTTCGTTGTTGGAACGGGGATCGGAAACTTTAATCCAGCCAAAGATCCAGCAAAATACAACTTGGTCGATCCTGTCGAGAGAAACACAGTTGGAGTTCCCACTGGAGGATGGACTGCCATCCGATTTAGAGCCGATAACCCAGGAGTCTGGTTCATGCATTGTCATCTTGAGGTTCATACAGGATGGGGTCTGAAAACAGCATTTGTGGTTGAAGATGGACCTGGAAAAGATCAAACCATTCTTCCACCCCCAAAGGATCTTCCACCATGCTAGCCTTTGTACCTGCCAAAACATAAACAAAGTGTTCTTTTGATTCCTGCTCAAGTTGCAGGAAATTTCTTTGCGACTTAAAATTACACTAAGATGCCAAAAA

Coding sequence (CDS)

ATGTTAACAATGGCCAGCTTGGTTTGGTTCGTTCATCGTCTGACATTGCTCTTCGTTGCCTGTTGTTTAGGATTAATCTCATTTTCTGCAGAAGCTGCAATAAAGAGATATCAGTTTGATGTTCAAGTGCAGAATATAAGTAGATTGTGTCATGCAAAGCCAATTGTCACCGTAAACGGGAAGTTTCCGGGGCCGACGATATATGTACAAGAAGGAGATCGAGTTCTTGTCAATGTTACAAACCATGCTCAATATAACATGACCTTAATGACACGAGTGAAAATTGAGCCACCCCCCACCCCCCAATATATGTGTGTATTTATATATGTTACTTGGGTTCTTATCTCAGTCGCCTTCTGCATATTCTGCAGGCATGGATTGAAGCAGTATCGAAACGGCTGGGCAGATGGACCTGCTTACATCACACAATGTCCTATTCAGAGAGGAAACAGCTATACCTATGATTTCAATGTCACAGGACAAAGAGGCACATTATGGTGGCATGCACACATCCTTTGGCTAAGGGCAACAGTTTATGGAGCAATTGTCATAATGCCCAAGCAAGGAACCCCTTATCCATTCCCTCAACCAAATAGAGAATTTGAAATTCTTCTGGGAGAATGGTGGAATAATGATGTTGAGGCGGTTGTCAACCAAGGATCCAGCATGGGCGTGCCACCAAATATGTCTGATGCTCACACAATCAATGGCAAGTCAGGGCCGCTCTTCCCTTGTTCGGAAAAACATACTTTCGCCATGGAGGTTGAAACTGGAAAGACATATCTCTTGAGAATAATAAATGCTGCCCTCAACGATGAGCTTTTCTTTGCCATTGCTGGTCACAACTTGACGGTCGTAGAGGTTGATGCAGTTTACACAAAATCATTCAGTACACAAGCTATACTAATTGCACCAGGACAGACCACAAATGTCTTGTTGCAAGCCAACCAAGCACCCAATAGATACTTCATGGCTTCCAGGTCGTTCATGGACGTTCCAATTCCTGTGGACAACAAAACAGCCACCGGCATTCTCCAATACAAAGGAATCCCTAACACAATCCTCCCAATCCTTCCCCAATTACCGGCCTCAAATGACACGTCATTTGCTTTAAACTACAACAAGAAGCTTAAAAGCTTAAACTCACCTCAATATCCTGCTAATGTTCCACTCAAAGTTGATCGAAAGCTTTTCTACACAATTGGTTTGGGCCAAAACGCTTGCCCTACTTGTCTAAATGGAACACAATTCGTTGCTTCATTAAACAATATCTCATTTGTGATGCCCCAAATTGGTCTTCTTCAATCACATTACTTCAATATCAGTGGGGTATTTAGAACTGATTTCCCAGACAGACCTCCAACTCCATTCAACTATACTGGAGCACCACTGACCGCTAATCTGAGAACTGCAGTGGGCACGAGACTTAGCAAGATTGCATTCAATTCTACGGTGGAGTTGGTACTACAGGACACCAATCTTCTTACTGTCGAGTCCCATCCATTCCATCTCCATGGCTACAACTTTTTCGTTGTTGGAACGGGGATCGGAAACTTTAATCCAGCCAAAGATCCAGCAAAATACAACTTGGTCGATCCTGTCGAGAGAAACACAGTTGGAGTTCCCACTGGAGGATGGACTGCCATCCGATTTAGAGCCGATAACCCAGGAGTCTGGTTCATGCATTGTCATCTTGAGGTTCATACAGGATGGGGTCTGAAAACAGCATTTGTGGTTGAAGATGGACCTGGAAAAGATCAAACCATTCTTCCACCCCCAAAGGATCTTCCACCATGCTAG

Protein sequence

MLTMASLVWFVHRLTLLFVACCLGLISFSAEAAIKRYQFDVQVQNISRLCHAKPIVTVNGKFPGPTIYVQEGDRVLVNVTNHAQYNMTLMTRVKIEPPPTPQYMCVFIYVTWVLISVAFCIFCRHGLKQYRNGWADGPAYITQCPIQRGNSYTYDFNVTGQRGTLWWHAHILWLRATVYGAIVIMPKQGTPYPFPQPNREFEILLGEWWNNDVEAVVNQGSSMGVPPNMSDAHTINGKSGPLFPCSEKHTFAMEVETGKTYLLRIINAALNDELFFAIAGHNLTVVEVDAVYTKSFSTQAILIAPGQTTNVLLQANQAPNRYFMASRSFMDVPIPVDNKTATGILQYKGIPNTILPILPQLPASNDTSFALNYNKKLKSLNSPQYPANVPLKVDRKLFYTIGLGQNACPTCLNGTQFVASLNNISFVMPQIGLLQSHYFNISGVFRTDFPDRPPTPFNYTGAPLTANLRTAVGTRLSKIAFNSTVELVLQDTNLLTVESHPFHLHGYNFFVVGTGIGNFNPAKDPAKYNLVDPVERNTVGVPTGGWTAIRFRADNPGVWFMHCHLEVHTGWGLKTAFVVEDGPGKDQTILPPPKDLPPC
Homology
BLAST of Lsi07G002590 vs. ExPASy Swiss-Prot
Match: Q8VZA1 (Laccase-11 OS=Arabidopsis thaliana OX=3702 GN=LAC11 PE=2 SV=1)

HSP 1 Score: 908.7 bits (2347), Expect = 3.5e-263
Identity = 422/589 (71.65%), Postives = 483/589 (82.00%), Query Frame = 0

Query: 13  RLTLLFVAC-CLGLISFS-AEAAIKRYQFDVQVQNISRLCHAKPIVTVNGKFPGPTIYVQ 72
           ++  LF+ C  L  + +S  +AA+K+YQFDVQV+NISR+C+AKPIVTVNG FPGPT+Y +
Sbjct: 2   KMGFLFLFCYLLAFLGYSPVDAAVKKYQFDVQVKNISRICNAKPIVTVNGMFPGPTVYAR 61

Query: 73  EGDRVLVNVTNHAQYNMTLMTRVKIEPPPTPQYMCVFIYVTWVLISVAFCIFCRHGLKQY 132
           EGDRV++NVTNH QYNM+                     + W            HGLKQY
Sbjct: 62  EGDRVIINVTNHVQYNMS---------------------IHW------------HGLKQY 121

Query: 133 RNGWADGPAYITQCPIQRGNSYTYDFNVTGQRGTLWWHAHILWLRATVYGAIVIMPKQGT 192
           RNGWADGPAYITQCPIQ G SY YDFNVTGQRGTLWWHAHILWLRATVYGAIVI+P  G 
Sbjct: 122 RNGWADGPAYITQCPIQTGQSYLYDFNVTGQRGTLWWHAHILWLRATVYGAIVILPAPGK 181

Query: 193 PYPFPQPNREFEILLGEWWNNDVEAVVNQGSSMGVPPNMSDAHTINGKSGPLFPCSEKHT 252
           PYPFPQP +E  I+LGEWWN DVE  VNQ + +G PP MSDAHTINGK GPLFPCSEKHT
Sbjct: 182 PYPFPQPYQESNIILGEWWNKDVETAVNQANQLGAPPPMSDAHTINGKPGPLFPCSEKHT 241

Query: 253 FAMEVETGKTYLLRIINAALNDELFFAIAGHNLTVVEVDAVYTKSFSTQAILIAPGQTTN 312
           F +E E GKTYLLRIINAALNDELFF IAGHN+TVVE+DAVYTK F+T+AIL+ PGQTTN
Sbjct: 242 FVIEAEAGKTYLLRIINAALNDELFFGIAGHNMTVVEIDAVYTKPFTTKAILLGPGQTTN 301

Query: 313 VLLQANQAPNRYFMASRSFMDVPIPVDNKTATGILQYKGIPNTILPILPQLPASNDTSFA 372
           VL++ +++PNRYFMA+  FMD P+ VDNKT T ILQYKG+PNT+LPILP+LP  NDTSFA
Sbjct: 302 VLVKTDRSPNRYFMAASPFMDAPVSVDNKTVTAILQYKGVPNTVLPILPKLPLPNDTSFA 361

Query: 373 LNYNKKLKSLNSPQYPANVPLKVDRKLFYTIGLGQNACPTCLNGTQFVASLNNISFVMPQ 432
           L+YN KLKSLN+P +PA VPLKVDR+LFYTIGLG NACPTC+NGT   AS+NNI+F+MP+
Sbjct: 362 LDYNGKLKSLNTPNFPALVPLKVDRRLFYTIGLGINACPTCVNGTNLAASINNITFIMPK 421

Query: 433 IGLLQSHYFNISGVFRTDFPDRPPTPFNYTGAPLTANLRTAVGTRLSKIAFNSTVELVLQ 492
             LL++HY NISGVFRTDFPDRPP  FNYTG PLTANL T+ GTRLS++ FN+T+ELVLQ
Sbjct: 422 TALLKAHYSNISGVFRTDFPDRPPKAFNYTGVPLTANLGTSTGTRLSRVKFNTTIELVLQ 481

Query: 493 DTNLLTVESHPFHLHGYNFFVVGTGIGNFNPAKDPAKYNLVDPVERNTVGVPTGGWTAIR 552
           DTNLLTVESHPFHLHGYNFFVVGTG+GNF+P KDPAK+NLVDP ERNTVGVPTGGW AIR
Sbjct: 482 DTNLLTVESHPFHLHGYNFFVVGTGVGNFDPKKDPAKFNLVDPPERNTVGVPTGGWAAIR 541

Query: 553 FRADNPGVWFMHCHLEVHTGWGLKTAFVVEDGPGKDQTILPPPKDLPPC 600
           FRADNPGVWFMHCHLEVHT WGLK AFVVE+G   + ++LPPPKD P C
Sbjct: 542 FRADNPGVWFMHCHLEVHTMWGLKMAFVVENGETPELSVLPPPKDYPSC 557

BLAST of Lsi07G002590 vs. ExPASy Swiss-Prot
Match: Q8RYM9 (Laccase-2 OS=Oryza sativa subsp. japonica OX=39947 GN=LAC2 PE=2 SV=1)

HSP 1 Score: 833.6 bits (2152), Expect = 1.4e-240
Identity = 389/588 (66.16%), Postives = 460/588 (78.23%), Query Frame = 0

Query: 14  LTLLFVACCLGLISFSAEAAIKRYQFDVQVQNISRLCHAKPIVTVNGKFPGPTIYVQEGD 73
           L LL  +  L L +  A A +KRYQFD+ + N+SRLCH K +VTVNG +PGPTIY +EGD
Sbjct: 8   LPLLVSSLLLALFALGAHADVKRYQFDIVMSNVSRLCHEKAMVTVNGSYPGPTIYAREGD 67

Query: 74  RVLVNVTNHAQYNMTLMTRVKIEPPPTPQYMCVFIYVTWVLISVAFCIFCRHGLKQYRNG 133
           RV+VNVTNH ++NMT                     + W            HGLKQ RNG
Sbjct: 68  RVIVNVTNHVKHNMT---------------------IHW------------HGLKQRRNG 127

Query: 134 WADGPAYITQCPIQRGNSYTYDFNVTGQRGTLWWHAHILWLRATVYGAIVIMPKQGTPYP 193
           WADGPAY+TQCPI  G SY YDFNVT QRGTLWWHAHI W+RATV+GAIVI+P  G PYP
Sbjct: 128 WADGPAYVTQCPIGSGGSYVYDFNVTRQRGTLWWHAHIAWMRATVHGAIVILPAAGVPYP 187

Query: 194 FPQPNREFEILLGEWWNNDVEAVVNQGSSMGVPPNMSDAHTINGKSGPLFP-CSEKHTFA 253
           FP+P+ E EI+LGEWW+ DVE V  QGS +G+ PNMSDAHTINGK GPL P CSEKHT+A
Sbjct: 188 FPKPDDEAEIVLGEWWHADVETVERQGSMLGMAPNMSDAHTINGKPGPLVPFCSEKHTYA 247

Query: 254 MEVETGKTYLLRIINAALNDELFFAIAGHNLTVVEVDAVYTKSFSTQAILIAPGQTTNVL 313
           ++V++GKTYLLRIINAA+NDELFF+IAGHN+TVVE+DA YTK F+   + ++PGQT NVL
Sbjct: 248 LQVQSGKTYLLRIINAAVNDELFFSIAGHNMTVVEIDATYTKPFAASTVQLSPGQTMNVL 307

Query: 314 LQANQAPNRYFMASRSFMDVPIPVDNKTATGILQYKGIPNTILPILPQ-LPASNDTSFAL 373
           + A+Q+P RYFM ++ F DVPIP DNKTAT ILQY G+P +++P LPQ +PA+N T    
Sbjct: 308 VSADQSPGRYFMVAKPFNDVPIPADNKTATAILQYAGVPTSVVPALPQTMPATNSTGSVA 367

Query: 374 NYNKKLKSLNSPQYPANVPLKVDRKLFYTIGLGQNACPTCLNGTQFVASLNNISFVMPQI 433
            ++ KL+SLNSP+YPA+VPL VDR L YTIGL  + C TCLN ++  ASLNNI+FVMP+ 
Sbjct: 368 AFHDKLRSLNSPRYPADVPLAVDRHLLYTIGLNIDPCETCLNRSRLAASLNNITFVMPRT 427

Query: 434 GLLQSHYFNISGVFRTDFPDRPPTPFNYTGAPLTANLRTAVGTRLSKIAFNSTVELVLQD 493
            LLQ+HY+   GVF  DFPDRPP  FNYTG PLTA L T++GTRLSKIA+N+TVELVLQD
Sbjct: 428 ALLQAHYYGQKGVFAADFPDRPPARFNYTGVPLTAGLGTSLGTRLSKIAYNATVELVLQD 487

Query: 494 TNLLTVESHPFHLHGYNFFVVGTGIGNFNPAKDPAKYNLVDPVERNTVGVPTGGWTAIRF 553
           TNLL+VESHPFHLHGYNFFVVG G+GNF+PAKDPAKYNLVDP ERNTVGVP GGWTAIRF
Sbjct: 488 TNLLSVESHPFHLHGYNFFVVGRGVGNFDPAKDPAKYNLVDPPERNTVGVPAGGWTAIRF 547

Query: 554 RADNPGVWFMHCHLEVHTGWGLKTAFVVEDGPGKDQTILPPPKDLPPC 600
           RADNPGVWF+HCHLEVHT WGLK AF+VEDG G D+++LPPPKDLP C
Sbjct: 548 RADNPGVWFLHCHLEVHTSWGLKMAFLVEDGSGPDESVLPPPKDLPKC 562

BLAST of Lsi07G002590 vs. ExPASy Swiss-Prot
Match: O80434 (Laccase-4 OS=Arabidopsis thaliana OX=3702 GN=IRX12 PE=2 SV=2)

HSP 1 Score: 708.4 bits (1827), Expect = 7.0e-203
Identity = 328/590 (55.59%), Postives = 420/590 (71.19%), Query Frame = 0

Query: 12  HRLTLLFVACCLGLISFSAEAAIKRYQFDVQVQNISRLCHAKPIVTVNGKFPGPTIYVQE 71
           H +  LF+     +    +E+ ++ Y+F+V ++N++RLC +KP VTVNG++PGPTIY +E
Sbjct: 4   HMVWFLFLVSFFSVFPAPSESMVRHYKFNVVMKNVTRLCSSKPTVTVNGRYPGPTIYARE 63

Query: 72  GDRVLVNVTNHAQYNMTLMTRVKIEPPPTPQYMCVFIYVTWVLISVAFCIFCRHGLKQYR 131
            D +L+ V NH +YN++                     + W            HG++Q R
Sbjct: 64  DDTLLIKVVNHVKYNVS---------------------IHW------------HGVRQVR 123

Query: 132 NGWADGPAYITQCPIQRGNSYTYDFNVTGQRGTLWWHAHILWLRATVYGAIVIMPKQGTP 191
            GWADGPAYITQCPIQ G  YTY++ +TGQRGTLWWHAHILWLRATVYGA+VI+PK+G P
Sbjct: 124 TGWADGPAYITQCPIQPGQVYTYNYTLTGQRGTLWWHAHILWLRATVYGALVILPKRGVP 183

Query: 192 YPFPQPNREFEILLGEWWNNDVEAVVNQGSSMGVPPNMSDAHTINGKSGPLFPCSEKHTF 251
           YPFP+P+ E  I+LGEWW +D E ++N+    G+ PN+SD+H ING  GP+  C  +  +
Sbjct: 184 YPFPKPDNEKVIVLGEWWKSDTENIINEALKSGLAPNVSDSHMINGHPGPVRNCPSQ-GY 243

Query: 252 AMEVETGKTYLLRIINAALNDELFFAIAGHNLTVVEVDAVYTKSFSTQAILIAPGQTTNV 311
            + VE GKTYLLR++NAALN+ELFF +AGH  TVVEVDAVY K F T  +LIAPGQTTNV
Sbjct: 244 KLSVENGKTYLLRLVNAALNEELFFKVAGHIFTVVEVDAVYVKPFKTDTVLIAPGQTTNV 303

Query: 312 LLQANQAPNRYFMASRSFMDVPIPVDNKTATGILQYKGIPNTILPILPQLPASNDTSFAL 371
           LL A+++  +Y + +  FMD PI VDN TAT  + Y G  ++   IL   P  N TS A 
Sbjct: 304 LLTASKSAGKYLVTASPFMDAPIAVDNVTATATVHYSGTLSSSPTILTLPPPQNATSIAN 363

Query: 372 NYNKKLKSLNSPQYPANVPLKVDRKLFYTIGLGQNACPTCL--NGTQFVASLNNISFVMP 431
           N+   L+SLNS +YPA VP  +D  LF+T+GLG NACPTC   NG++ VAS+NN++F+MP
Sbjct: 364 NFTNSLRSLNSKKYPALVPTTIDHHLFFTVGLGLNACPTCKAGNGSRVVASINNVTFIMP 423

Query: 432 QIGLLQSHYFNISGVFRTDFPDRPPTPFNYTGAPLTANLRTAVGTRLSKIAFNSTVELVL 491
           +  LL +HYFN SGVF TDFP  PP  FNY+G  +T N+ T  GTRL K+ +N+TV+LVL
Sbjct: 424 KTALLPAHYFNTSGVFTTDFPKNPPHVFNYSGGSVT-NMATETGTRLYKLPYNATVQLVL 483

Query: 492 QDTNLLTVESHPFHLHGYNFFVVGTGIGNFNPAKDPAKYNLVDPVERNTVGVPTGGWTAI 551
           QDT ++  E+HP HLHG+NFF VG G+GNFN  KDP  +NLVDPVERNT+GVP+GGW  I
Sbjct: 484 QDTGVIAPENHPVHLHGFNFFEVGRGLGNFNSTKDPKNFNLVDPVERNTIGVPSGGWVVI 543

Query: 552 RFRADNPGVWFMHCHLEVHTGWGLKTAFVVEDGPGKDQTILPPPKDLPPC 600
           RFRADNPGVWFMHCHLEVHT WGLK AF+VE+G G +Q+ILPPPKDLP C
Sbjct: 544 RFRADNPGVWFMHCHLEVHTTWGLKMAFLVENGKGPNQSILPPPKDLPKC 558

BLAST of Lsi07G002590 vs. ExPASy Swiss-Prot
Match: Q0IQU1 (Laccase-22 OS=Oryza sativa subsp. japonica OX=39947 GN=LAC22 PE=2 SV=2)

HSP 1 Score: 703.7 bits (1815), Expect = 1.7e-201
Identity = 328/590 (55.59%), Postives = 416/590 (70.51%), Query Frame = 0

Query: 13  RLTLLFVACCLGLISFSAEAAIKRYQFDVQVQNISRLCHAKPIVTVNGKFPGPTIYVQEG 72
           RL+LL +A C  L + SA A  + Y+F+V ++N++RLC  KPI+TVNGKFPGPT+Y +EG
Sbjct: 9   RLSLLLMAACFLLQALSAHAITRHYKFNVVMRNMTRLCSTKPILTVNGKFPGPTLYAREG 68

Query: 73  DRVLVNVTNHAQYNMTLMTRVKIEPPPTPQYMCVFIYVTWVLISVAFCIFCRHGLKQYRN 132
           D VLV V NH  +N+T                     + W            HG++Q R 
Sbjct: 69  DNVLVKVVNHVAHNVT---------------------IHW------------HGVRQIRT 128

Query: 133 GWADGPAYITQCPIQRGNSYTYDFNVTGQRGTLWWHAHILWLRATVYGAIVIMPKQGTPY 192
           GW DGPAYITQCPIQ G+S+ Y+F +TGQRGTL WHAHI WLRATV+GAIVI+PK G PY
Sbjct: 129 GWYDGPAYITQCPIQPGSSFLYNFTITGQRGTLLWHAHINWLRATVHGAIVILPKLGVPY 188

Query: 193 PFPQPNREFEILLGEWWNNDVEAVVNQGSSMGVPPNMSDAHTINGKSGPLFPC-SEKHTF 252
           PFP P++E  I+LGEWW  D E V+NQ   +GV PN+SD+HTING  GPL  C S +  F
Sbjct: 189 PFPAPHKEAVIVLGEWWKEDTETVINQAMQLGVGPNISDSHTINGHPGPLSECASSQDGF 248

Query: 253 AMEVETGKTYLLRIINAALNDELFFAIAGHNLTVVEVDAVYTKSFSTQAILIAPGQTTNV 312
            + VE GKTY+LRIINAALND+LFF +AGH LTVVEVDAVYTK F T  +LI PGQTTNV
Sbjct: 249 KLSVENGKTYMLRIINAALNDDLFFKVAGHELTVVEVDAVYTKPFKTDTLLITPGQTTNV 308

Query: 313 LLQANQAPNRYFMASRSFMDVPIPVDNKTATGILQYKGIPNTILPILPQL--PASNDTSF 372
           L++ANQ   RY ++   FMD P+ VDNKT T  L Y    ++ +  L  +  P  N T  
Sbjct: 309 LVRANQGAGRYLLSVSPFMDAPVQVDNKTGTATLHYANTVSSSMASLTLVKPPPQNATHI 368

Query: 373 ALNYNKKLKSLNSPQYPANVPLKVDRKLFYTIGLGQNACPTCLNGTQFVASLNNISFVMP 432
              +   L SLNS +YPANVP  VD  L  T+G+G N CP+C+NGT+ V ++NN++F+MP
Sbjct: 369 VSKFTDSLHSLNSKEYPANVPQTVDHSLLLTVGVGVNPCPSCINGTRVVGTINNVTFIMP 428

Query: 433 QIGLLQSHYFNISGVFRTDFPDRPPTPFNYTGAPLTANLRTAVGTRLSKIAFNSTVELVL 492
              +LQ+HY+NI GVF  DFP  P   FNYTG+    NL+T  GTR+ ++ +N++V++VL
Sbjct: 429 STPILQAHYYNIPGVFTEDFPATPLHKFNYTGSG-PKNLQTMNGTRVYRLPYNASVQVVL 488

Query: 493 QDTNLLTVESHPFHLHGYNFFVVGTGIGNFNPAKDPAKYNLVDPVERNTVGVPTGGWTAI 552
           QDT +++ ESHP HLHG+NFFVVG G+GN+NP   P+ +NL+DP+ERNT+GVPTGGWTAI
Sbjct: 489 QDTGIISPESHPIHLHGFNFFVVGKGVGNYNPRTSPSTFNLIDPIERNTIGVPTGGWTAI 548

Query: 553 RFRADNPGVWFMHCHLEVHTGWGLKTAFVVEDGPGKDQTILPPPKDLPPC 600
           RFR+DNPGVWFMHCH EVHT WGLK AFVV++G    +T++PPPKDLP C
Sbjct: 549 RFRSDNPGVWFMHCHFEVHTSWGLKMAFVVDNGKRPSETLIPPPKDLPQC 564

BLAST of Lsi07G002590 vs. ExPASy Swiss-Prot
Match: Q1PDH6 (Laccase-16 OS=Arabidopsis thaliana OX=3702 GN=LAC16 PE=2 SV=2)

HSP 1 Score: 680.6 bits (1755), Expect = 1.6e-194
Identity = 324/594 (54.55%), Postives = 412/594 (69.36%), Query Frame = 0

Query: 18  FVACCLGLISF------SAEAAIKRYQFDVQVQNISRLCHAKPIVTVNGKFPGPTIYVQE 77
           F  C L    F      +  + I+ Y+F+V + N ++LC +KPIVTVNG+FPGPTI  +E
Sbjct: 7   FCICVLTFFVFVLLSPTTVHSIIRHYKFNV-MTNTTKLCSSKPIVTVNGQFPGPTIVARE 66

Query: 78  GDRVLVNVTNHAQYNMTLMTRVKIEPPPTPQYMCVFIYVTWVLISVAFCIFCRHGLKQYR 137
           GD +L+ V NH +YN++                     + W            HG++Q R
Sbjct: 67  GDTILIKVVNHVKYNVS---------------------IHW------------HGIRQLR 126

Query: 138 NGWADGPAYITQCPIQRGNSYTYDFNVTGQRGTLWWHAHILWLRATVYGAIVIMPKQGTP 197
            GWADGPAYITQCPIQ G +Y ++F +TGQRGTLWWHAHILWLRATV+GAIVI+PK G P
Sbjct: 127 TGWADGPAYITQCPIQPGQNYLHNFTLTGQRGTLWWHAHILWLRATVHGAIVILPKLGVP 186

Query: 198 YPFPQPNREFEILLGEWWNNDVEAVVNQGSSMGVPPNMSDAHTINGKSGPLFPCSEKHTF 257
           YPFP+P +E  I+L EWW +DVE ++N+ S +G  P+ SDAHTING SG +  C  + ++
Sbjct: 187 YPFPKPYKEKTIVLSEWWKSDVEELINEASRIGTAPSASDAHTINGHSGSISNCPSQSSY 246

Query: 258 AMEVETGKTYLLRIINAALNDELFFAIAGHNLTVVEVDAVYTKSFSTQAILIAPGQTTNV 317
            + V  GKTY+LRIINAALN+ELFF IAGH LTVVEVDAVYTK + T  + IAPGQTTNV
Sbjct: 247 GLPVRAGKTYMLRIINAALNEELFFKIAGHVLTVVEVDAVYTKPYKTDTVFIAPGQTTNV 306

Query: 318 LLQAN-QAPNRYFMASRSFMDVPIPVDNKTATGILQYKGIPNTI----LPILPQLPASND 377
           LL AN  A + Y +A+ +F D  IP DN TAT  L Y G  +T+      +L  LP  N 
Sbjct: 307 LLTANANAGSNYMVAATTFTDAHIPYDNVTATATLHYIGHTSTVSTSKKTVLASLPPQNA 366

Query: 378 TSFALNYNKKLKSLNSPQYPANVPLKVDRKLFYTIGLGQNACPTCLNGTQFVASLNNISF 437
           T  A  + + L+SLNS +YPA VP  V+  LF+T+GLG N C +C NG + VA +NN++F
Sbjct: 367 TWVATKFTRSLRSLNSLEYPARVPTTVEHSLFFTVGLGANPCQSCNNGVRLVAGINNVTF 426

Query: 438 VMPQIGLLQSHYFNISGVFRTDFPDRPPTPFNYTG-APLTANLRTAVGTRLSKIAFNSTV 497
            MP+  LLQ+H+FNISGVF  DFP +P  P++YT    L  N  T  GT+L ++ +N+TV
Sbjct: 427 TMPKTALLQAHFFNISGVFTDDFPAKPSNPYDYTAPVKLGVNAATMKGTKLYRLPYNATV 486

Query: 498 ELVLQDTNLLTVESHPFHLHGYNFFVVGTGIGNFNPAKDPAKYNLVDPVERNTVGVPTGG 557
           ++VLQ+T ++  ++HPFHLHG+NFF VG G+GNFNP KDP  +NLVDPVERNTVGVP GG
Sbjct: 487 QIVLQNTAMILSDNHPFHLHGFNFFEVGRGLGNFNPEKDPKAFNLVDPVERNTVGVPAGG 546

Query: 558 WTAIRFRADNPGVWFMHCHLEVHTGWGLKTAFVVEDGPGKDQTILPPPKDLPPC 600
           WTAIRF ADNPGVWFMHCHLE+HT WGLK AFVV++G G DQ++LPPP DLP C
Sbjct: 547 WTAIRFIADNPGVWFMHCHLELHTTWGLKMAFVVDNGHGPDQSLLPPPADLPKC 566

BLAST of Lsi07G002590 vs. ExPASy TrEMBL
Match: A0A5D3D2C1 (Laccase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold21G004900 PE=3 SV=1)

HSP 1 Score: 1118.2 bits (2891), Expect = 0.0e+00
Identity = 540/596 (90.60%), Postives = 549/596 (92.11%), Query Frame = 0

Query: 4   MASLVWFVHRLTLLFVACCLGLISFSAEAAIKRYQFDVQVQNISRLCHAKPIVTVNGKFP 63
           MA LVW VHRLT LFVA CLG ISFS EAAIKRYQFDVQVQNISRLCHAKPIVTVNGKFP
Sbjct: 1   MAGLVWVVHRLTFLFVAYCLGFISFSVEAAIKRYQFDVQVQNISRLCHAKPIVTVNGKFP 60

Query: 64  GPTIYVQEGDRVLVNVTNHAQYNMTLMTRVKIEPPPTPQYMCVFIYVTWVLISVAFCIFC 123
           GPTIYVQEGDRVLVNVTNHAQYNM+                     + W           
Sbjct: 61  GPTIYVQEGDRVLVNVTNHAQYNMS---------------------IHW----------- 120

Query: 124 RHGLKQYRNGWADGPAYITQCPIQRGNSYTYDFNVTGQRGTLWWHAHILWLRATVYGAIV 183
            HGLKQYRNGWADGPAYITQCPIQRGNSYTYDFNVTGQRGTLWWHAHILWLRATVYGAIV
Sbjct: 121 -HGLKQYRNGWADGPAYITQCPIQRGNSYTYDFNVTGQRGTLWWHAHILWLRATVYGAIV 180

Query: 184 IMPKQGTPYPFPQPNREFEILLGEWWNNDVEAVVNQGSSMGVPPNMSDAHTINGKSGPLF 243
           I+PKQGTPYPFPQPN EFEILLGEWWNNDVEAVVNQGS MGVPPNMSDAHTINGK GPLF
Sbjct: 181 ILPKQGTPYPFPQPNGEFEILLGEWWNNDVEAVVNQGSRMGVPPNMSDAHTINGKPGPLF 240

Query: 244 PCSEKHTFAMEVETGKTYLLRIINAALNDELFFAIAGHNLTVVEVDAVYTKSFSTQAILI 303
           PCSEKHTFAMEVE GKTYLLRIINAALNDELFFAIAGHN+TVVEVDAVYTK F+TQAILI
Sbjct: 241 PCSEKHTFAMEVEAGKTYLLRIINAALNDELFFAIAGHNMTVVEVDAVYTKPFTTQAILI 300

Query: 304 APGQTTNVLLQANQAPNRYFMASRSFMDVPIPVDNKTATGILQYKGIPNTILPILPQLPA 363
           APGQTTNVLLQANQAPNRYFMASRSFMDVPIPVDNKTATGILQY+GIPNTILPILPQLPA
Sbjct: 301 APGQTTNVLLQANQAPNRYFMASRSFMDVPIPVDNKTATGILQYRGIPNTILPILPQLPA 360

Query: 364 SNDTSFALNYNKKLKSLNSPQYPANVPLKVDRKLFYTIGLGQNACPTCLNGTQFVASLNN 423
           SNDTSFAL+YNKKLKSLNSPQYPANVPL+VDRKLFYTIGLGQN CPTCLNGTQ VASLNN
Sbjct: 361 SNDTSFALSYNKKLKSLNSPQYPANVPLEVDRKLFYTIGLGQNDCPTCLNGTQLVASLNN 420

Query: 424 ISFVMPQIGLLQSHYFNISGVFRTDFPDRPPTPFNYTGAPLTANLRTAVGTRLSKIAFNS 483
           ISFVMPQIGLLQSHYFNISGVFRTDFPDRPPTPFNYTGAPLTANLRTAVGTRLSKIAFNS
Sbjct: 421 ISFVMPQIGLLQSHYFNISGVFRTDFPDRPPTPFNYTGAPLTANLRTAVGTRLSKIAFNS 480

Query: 484 TVELVLQDTNLLTVESHPFHLHGYNFFVVGTGIGNFNPAKDPAKYNLVDPVERNTVGVPT 543
           TVELVLQDTNLLTVESHPFHLHGYNFFVVGTGIGNFNPAKDPAKYNLVDPVERNTVGVPT
Sbjct: 481 TVELVLQDTNLLTVESHPFHLHGYNFFVVGTGIGNFNPAKDPAKYNLVDPVERNTVGVPT 540

Query: 544 GGWTAIRFRADNPGVWFMHCHLEVHTGWGLKTAFVVEDGPGKDQTILPPPKDLPPC 600
           GGWTAIRFRADNPGVWFMHCHLEVHTGWGLKTAFVVEDGPGKDQ+ILPPPKDLPPC
Sbjct: 541 GGWTAIRFRADNPGVWFMHCHLEVHTGWGLKTAFVVEDGPGKDQSILPPPKDLPPC 563

BLAST of Lsi07G002590 vs. ExPASy TrEMBL
Match: A0A1S3BU50 (Laccase OS=Cucumis melo OX=3656 GN=LOC103493188 PE=3 SV=1)

HSP 1 Score: 1118.2 bits (2891), Expect = 0.0e+00
Identity = 540/596 (90.60%), Postives = 549/596 (92.11%), Query Frame = 0

Query: 4   MASLVWFVHRLTLLFVACCLGLISFSAEAAIKRYQFDVQVQNISRLCHAKPIVTVNGKFP 63
           MA LVW VHRLT LFVA CLG ISFS EAAIKRYQFDVQVQNISRLCHAKPIVTVNGKFP
Sbjct: 1   MAGLVWVVHRLTFLFVAYCLGFISFSVEAAIKRYQFDVQVQNISRLCHAKPIVTVNGKFP 60

Query: 64  GPTIYVQEGDRVLVNVTNHAQYNMTLMTRVKIEPPPTPQYMCVFIYVTWVLISVAFCIFC 123
           GPTIYVQEGDRVLVNVTNHAQYNM+                     + W           
Sbjct: 61  GPTIYVQEGDRVLVNVTNHAQYNMS---------------------IHW----------- 120

Query: 124 RHGLKQYRNGWADGPAYITQCPIQRGNSYTYDFNVTGQRGTLWWHAHILWLRATVYGAIV 183
            HGLKQYRNGWADGPAYITQCPIQRGNSYTYDFNVTGQRGTLWWHAHILWLRATVYGAIV
Sbjct: 121 -HGLKQYRNGWADGPAYITQCPIQRGNSYTYDFNVTGQRGTLWWHAHILWLRATVYGAIV 180

Query: 184 IMPKQGTPYPFPQPNREFEILLGEWWNNDVEAVVNQGSSMGVPPNMSDAHTINGKSGPLF 243
           I+PKQGTPYPFPQPN EFEILLGEWWNNDVEAVVNQGS MGVPPNMSDAHTINGK GPLF
Sbjct: 181 ILPKQGTPYPFPQPNGEFEILLGEWWNNDVEAVVNQGSRMGVPPNMSDAHTINGKPGPLF 240

Query: 244 PCSEKHTFAMEVETGKTYLLRIINAALNDELFFAIAGHNLTVVEVDAVYTKSFSTQAILI 303
           PCSEKHTFAMEVE GKTYLLRIINAALNDELFFAIAGHN+TVVEVDAVYTK F+TQAILI
Sbjct: 241 PCSEKHTFAMEVEAGKTYLLRIINAALNDELFFAIAGHNMTVVEVDAVYTKPFTTQAILI 300

Query: 304 APGQTTNVLLQANQAPNRYFMASRSFMDVPIPVDNKTATGILQYKGIPNTILPILPQLPA 363
           APGQTTNVLLQANQAPNRYFMASRSFMDVPIPVDNKTATGILQY+GIPNTILPILPQLPA
Sbjct: 301 APGQTTNVLLQANQAPNRYFMASRSFMDVPIPVDNKTATGILQYRGIPNTILPILPQLPA 360

Query: 364 SNDTSFALNYNKKLKSLNSPQYPANVPLKVDRKLFYTIGLGQNACPTCLNGTQFVASLNN 423
           SNDTSFAL+YNKKLKSLNSPQYPANVPL+VDRKLFYTIGLGQN CPTCLNGTQ VASLNN
Sbjct: 361 SNDTSFALSYNKKLKSLNSPQYPANVPLEVDRKLFYTIGLGQNDCPTCLNGTQLVASLNN 420

Query: 424 ISFVMPQIGLLQSHYFNISGVFRTDFPDRPPTPFNYTGAPLTANLRTAVGTRLSKIAFNS 483
           ISFVMPQIGLLQSHYFNISGVFRTDFPDRPPTPFNYTGAPLTANLRTAVGTRLSKIAFNS
Sbjct: 421 ISFVMPQIGLLQSHYFNISGVFRTDFPDRPPTPFNYTGAPLTANLRTAVGTRLSKIAFNS 480

Query: 484 TVELVLQDTNLLTVESHPFHLHGYNFFVVGTGIGNFNPAKDPAKYNLVDPVERNTVGVPT 543
           TVELVLQDTNLLTVESHPFHLHGYNFFVVGTGIGNFNPAKDPAKYNLVDPVERNTVGVPT
Sbjct: 481 TVELVLQDTNLLTVESHPFHLHGYNFFVVGTGIGNFNPAKDPAKYNLVDPVERNTVGVPT 540

Query: 544 GGWTAIRFRADNPGVWFMHCHLEVHTGWGLKTAFVVEDGPGKDQTILPPPKDLPPC 600
           GGWTAIRFRADNPGVWFMHCHLEVHTGWGLKTAFVVEDGPGKDQ+ILPPPKDLPPC
Sbjct: 541 GGWTAIRFRADNPGVWFMHCHLEVHTGWGLKTAFVVEDGPGKDQSILPPPKDLPPC 563

BLAST of Lsi07G002590 vs. ExPASy TrEMBL
Match: A0A0A0KWQ3 (Laccase OS=Cucumis sativus OX=3659 GN=Csa_4G046770 PE=3 SV=1)

HSP 1 Score: 1116.7 bits (2887), Expect = 0.0e+00
Identity = 537/596 (90.10%), Postives = 550/596 (92.28%), Query Frame = 0

Query: 4   MASLVWFVHRLTLLFVACCLGLISFSAEAAIKRYQFDVQVQNISRLCHAKPIVTVNGKFP 63
           MASLVWFVHRLT LFVA C+GLISFS EAAIKRYQFDVQVQNISRLCHAKPIVTVNGKFP
Sbjct: 1   MASLVWFVHRLTFLFVASCIGLISFSVEAAIKRYQFDVQVQNISRLCHAKPIVTVNGKFP 60

Query: 64  GPTIYVQEGDRVLVNVTNHAQYNMTLMTRVKIEPPPTPQYMCVFIYVTWVLISVAFCIFC 123
           GPTIYVQEGDRVLVNVTNHAQYNM+                     + W           
Sbjct: 61  GPTIYVQEGDRVLVNVTNHAQYNMS---------------------IHW----------- 120

Query: 124 RHGLKQYRNGWADGPAYITQCPIQRGNSYTYDFNVTGQRGTLWWHAHILWLRATVYGAIV 183
            HGLKQYRNGWADGPAYITQCPIQRGNSYTYDFNVTGQRGTLWWHAHILWLRATVYGAIV
Sbjct: 121 -HGLKQYRNGWADGPAYITQCPIQRGNSYTYDFNVTGQRGTLWWHAHILWLRATVYGAIV 180

Query: 184 IMPKQGTPYPFPQPNREFEILLGEWWNNDVEAVVNQGSSMGVPPNMSDAHTINGKSGPLF 243
           I+PKQGTPYPFPQPN EFEILLGEWWNNDVEAVVNQGS MG+PPNMSDAHTINGK GPLF
Sbjct: 181 ILPKQGTPYPFPQPNGEFEILLGEWWNNDVEAVVNQGSRMGLPPNMSDAHTINGKPGPLF 240

Query: 244 PCSEKHTFAMEVETGKTYLLRIINAALNDELFFAIAGHNLTVVEVDAVYTKSFSTQAILI 303
           PCSEKHTFAMEVE GKTYLLRIINAALNDELFFAIAGH++TVVEVDAVYTK F+TQAILI
Sbjct: 241 PCSEKHTFAMEVEAGKTYLLRIINAALNDELFFAIAGHSMTVVEVDAVYTKPFTTQAILI 300

Query: 304 APGQTTNVLLQANQAPNRYFMASRSFMDVPIPVDNKTATGILQYKGIPNTILPILPQLPA 363
           APGQTTNVLLQANQAPNRYFMASRSFMDVPIPVDNKTATGILQY+GIPNT+LP LPQLPA
Sbjct: 301 APGQTTNVLLQANQAPNRYFMASRSFMDVPIPVDNKTATGILQYRGIPNTVLPTLPQLPA 360

Query: 364 SNDTSFALNYNKKLKSLNSPQYPANVPLKVDRKLFYTIGLGQNACPTCLNGTQFVASLNN 423
           SNDTSFAL+YNKKLKSLNSPQYPANVPLKVDRKLFYTIGLGQN CPTCLNGTQ VASLNN
Sbjct: 361 SNDTSFALSYNKKLKSLNSPQYPANVPLKVDRKLFYTIGLGQNECPTCLNGTQLVASLNN 420

Query: 424 ISFVMPQIGLLQSHYFNISGVFRTDFPDRPPTPFNYTGAPLTANLRTAVGTRLSKIAFNS 483
           ISFVMPQIGLLQSHYFNI GVFRTDFPDRPPTPFNYTGAPLTANLRTAVGTRLSKIAFNS
Sbjct: 421 ISFVMPQIGLLQSHYFNIGGVFRTDFPDRPPTPFNYTGAPLTANLRTAVGTRLSKIAFNS 480

Query: 484 TVELVLQDTNLLTVESHPFHLHGYNFFVVGTGIGNFNPAKDPAKYNLVDPVERNTVGVPT 543
           TVELVLQDTNLLTVESHPFHLHGYNFFVVGTGIGNF+PAKDPAKYNLVDPVERNTVGVPT
Sbjct: 481 TVELVLQDTNLLTVESHPFHLHGYNFFVVGTGIGNFDPAKDPAKYNLVDPVERNTVGVPT 540

Query: 544 GGWTAIRFRADNPGVWFMHCHLEVHTGWGLKTAFVVEDGPGKDQTILPPPKDLPPC 600
           GGWTAIRFRADNPGVWFMHCHLEVHTGWGLKTAFVVEDGPGKDQ+ILPPPKDLPPC
Sbjct: 541 GGWTAIRFRADNPGVWFMHCHLEVHTGWGLKTAFVVEDGPGKDQSILPPPKDLPPC 563

BLAST of Lsi07G002590 vs. ExPASy TrEMBL
Match: A0A6J1EN14 (Laccase OS=Cucurbita moschata OX=3662 GN=LOC111435985 PE=3 SV=1)

HSP 1 Score: 1113.2 bits (2878), Expect = 0.0e+00
Identity = 532/598 (88.96%), Postives = 552/598 (92.31%), Query Frame = 0

Query: 2   LTMASLVWFVHRLTLLFVACCLGLISFSAEAAIKRYQFDVQVQNISRLCHAKPIVTVNGK 61
           +TMASLV FV RLT +FVACC GLISFS EAAIKRYQFDVQVQNISRLCHAKPIVT+NG+
Sbjct: 1   MTMASLVCFVRRLTFVFVACCFGLISFSVEAAIKRYQFDVQVQNISRLCHAKPIVTINGR 60

Query: 62  FPGPTIYVQEGDRVLVNVTNHAQYNMTLMTRVKIEPPPTPQYMCVFIYVTWVLISVAFCI 121
           FPGPTIYVQEGDRVLVNVTNHAQYNM+                     + W         
Sbjct: 61  FPGPTIYVQEGDRVLVNVTNHAQYNMS---------------------IHW--------- 120

Query: 122 FCRHGLKQYRNGWADGPAYITQCPIQRGNSYTYDFNVTGQRGTLWWHAHILWLRATVYGA 181
              HGLKQYRNGWADGPAYITQCPIQRG+SYTYDFNVTGQRGTLWWHAHILWLRATVYGA
Sbjct: 121 ---HGLKQYRNGWADGPAYITQCPIQRGSSYTYDFNVTGQRGTLWWHAHILWLRATVYGA 180

Query: 182 IVIMPKQGTPYPFPQPNREFEILLGEWWNNDVEAVVNQGSSMGVPPNMSDAHTINGKSGP 241
           IVI+PKQGTPYPFPQPNREFEILLGEWWNNDVEA+VNQGSSMGVPPNMSDAHTINGK GP
Sbjct: 181 IVILPKQGTPYPFPQPNREFEILLGEWWNNDVEAIVNQGSSMGVPPNMSDAHTINGKPGP 240

Query: 242 LFPCSEKHTFAMEVETGKTYLLRIINAALNDELFFAIAGHNLTVVEVDAVYTKSFSTQAI 301
           LFPCSEKHTFAMEVE GKTYLLRIINAALNDELFFAIAGHN+TVVEVDAVYTK F+TQAI
Sbjct: 241 LFPCSEKHTFAMEVEAGKTYLLRIINAALNDELFFAIAGHNMTVVEVDAVYTKPFTTQAI 300

Query: 302 LIAPGQTTNVLLQANQAPNRYFMASRSFMDVPIPVDNKTATGILQYKGIPNTILPILPQL 361
           LIAPGQTTNVL+QANQ PNRYFMASRSFMDVPIPVDNKTATGILQYKGIPNTILP+LPQL
Sbjct: 301 LIAPGQTTNVLVQANQPPNRYFMASRSFMDVPIPVDNKTATGILQYKGIPNTILPVLPQL 360

Query: 362 PASNDTSFALNYNKKLKSLNSPQYPANVPLKVDRKLFYTIGLGQNACPTCLNGTQFVASL 421
           PASND+SFAL+YNKK+KSLNSPQYPANVPLKVDRKLFYTIGLGQNACPTC+NGTQ VASL
Sbjct: 361 PASNDSSFALSYNKKIKSLNSPQYPANVPLKVDRKLFYTIGLGQNACPTCINGTQLVASL 420

Query: 422 NNISFVMPQIGLLQSHYFNISGVFRTDFPDRPPTPFNYTGAPLTANLRTAVGTRLSKIAF 481
           NNISFVMPQIGLLQSHYFNISGVFR DFPDRPPTPFNYTGAPLTANLRTAVGTRL KIAF
Sbjct: 421 NNISFVMPQIGLLQSHYFNISGVFRADFPDRPPTPFNYTGAPLTANLRTAVGTRLGKIAF 480

Query: 482 NSTVELVLQDTNLLTVESHPFHLHGYNFFVVGTGIGNFNPAKDPAKYNLVDPVERNTVGV 541
           NSTVELVLQDTNLLTVESHPFHLHGYNFFVVGTGIGNF+PAKDPAKYNLVDP+ERNTVGV
Sbjct: 481 NSTVELVLQDTNLLTVESHPFHLHGYNFFVVGTGIGNFDPAKDPAKYNLVDPIERNTVGV 540

Query: 542 PTGGWTAIRFRADNPGVWFMHCHLEVHTGWGLKTAFVVEDGPGKDQTILPPPKDLPPC 600
           PTGGWTAIRFRADNPGVWFMHCHLEVHTGWGLKTAFVVEDGPGKDQ+ILPPPKDLPPC
Sbjct: 541 PTGGWTAIRFRADNPGVWFMHCHLEVHTGWGLKTAFVVEDGPGKDQSILPPPKDLPPC 565

BLAST of Lsi07G002590 vs. ExPASy TrEMBL
Match: A0A6J1JB06 (Laccase OS=Cucurbita maxima OX=3661 GN=LOC111482840 PE=3 SV=1)

HSP 1 Score: 1110.9 bits (2872), Expect = 0.0e+00
Identity = 532/596 (89.26%), Postives = 550/596 (92.28%), Query Frame = 0

Query: 4   MASLVWFVHRLTLLFVACCLGLISFSAEAAIKRYQFDVQVQNISRLCHAKPIVTVNGKFP 63
           MASLV FV RLT LFV CC GLISFSAEAA+KRYQFDVQVQNISRLCHAKPIVT+NG+FP
Sbjct: 1   MASLVCFVRRLTFLFVTCCFGLISFSAEAAVKRYQFDVQVQNISRLCHAKPIVTINGRFP 60

Query: 64  GPTIYVQEGDRVLVNVTNHAQYNMTLMTRVKIEPPPTPQYMCVFIYVTWVLISVAFCIFC 123
           GPTIYVQEGDRVLVNVTNHAQYNM+                     + W           
Sbjct: 61  GPTIYVQEGDRVLVNVTNHAQYNMS---------------------IHW----------- 120

Query: 124 RHGLKQYRNGWADGPAYITQCPIQRGNSYTYDFNVTGQRGTLWWHAHILWLRATVYGAIV 183
            HGLKQYRNGWADGPAYITQCPIQRG+SYTYDFNVTGQRGTLWWHAHILWLRATVYGAIV
Sbjct: 121 -HGLKQYRNGWADGPAYITQCPIQRGSSYTYDFNVTGQRGTLWWHAHILWLRATVYGAIV 180

Query: 184 IMPKQGTPYPFPQPNREFEILLGEWWNNDVEAVVNQGSSMGVPPNMSDAHTINGKSGPLF 243
           I+PKQGTPYPFPQPNREFEILLGEWWNNDVEAVVNQGSSMGVPPNMSDAHTINGK GPLF
Sbjct: 181 ILPKQGTPYPFPQPNREFEILLGEWWNNDVEAVVNQGSSMGVPPNMSDAHTINGKPGPLF 240

Query: 244 PCSEKHTFAMEVETGKTYLLRIINAALNDELFFAIAGHNLTVVEVDAVYTKSFSTQAILI 303
           PCSEKHTFAMEVE GKTYLLRIINAALNDELFFAIAGHN+TVVEVDAVYTK F+TQAILI
Sbjct: 241 PCSEKHTFAMEVEAGKTYLLRIINAALNDELFFAIAGHNMTVVEVDAVYTKPFTTQAILI 300

Query: 304 APGQTTNVLLQANQAPNRYFMASRSFMDVPIPVDNKTATGILQYKGIPNTILPILPQLPA 363
           APGQTTNVL+QANQ PNRYFMASRSFMDVPIPVDNKTATGILQYKGIPNTILPILPQLPA
Sbjct: 301 APGQTTNVLVQANQPPNRYFMASRSFMDVPIPVDNKTATGILQYKGIPNTILPILPQLPA 360

Query: 364 SNDTSFALNYNKKLKSLNSPQYPANVPLKVDRKLFYTIGLGQNACPTCLNGTQFVASLNN 423
           SND+SFAL+YNKK+KSLN+PQYPANVPLKVDRKLFYTIGLGQNACPTC+NGTQ VASLNN
Sbjct: 361 SNDSSFALSYNKKIKSLNTPQYPANVPLKVDRKLFYTIGLGQNACPTCVNGTQLVASLNN 420

Query: 424 ISFVMPQIGLLQSHYFNISGVFRTDFPDRPPTPFNYTGAPLTANLRTAVGTRLSKIAFNS 483
           ISFVMPQIGLLQSHYFNISGVFR DFPDRPPTPFNYTGAPLTANLRTAVGTRL KIAFNS
Sbjct: 421 ISFVMPQIGLLQSHYFNISGVFRADFPDRPPTPFNYTGAPLTANLRTAVGTRLGKIAFNS 480

Query: 484 TVELVLQDTNLLTVESHPFHLHGYNFFVVGTGIGNFNPAKDPAKYNLVDPVERNTVGVPT 543
           TVELVLQDTNLLTVESHPFHLHGYNFFVVGTGIGNF+PAKDPAKYNLVDP+ERNTVGVPT
Sbjct: 481 TVELVLQDTNLLTVESHPFHLHGYNFFVVGTGIGNFDPAKDPAKYNLVDPIERNTVGVPT 540

Query: 544 GGWTAIRFRADNPGVWFMHCHLEVHTGWGLKTAFVVEDGPGKDQTILPPPKDLPPC 600
           GGWTAIRFRADNPGVWFMHCHLEVHTGWGLKTAFVVEDGPGKDQ+ILPPPKDLPPC
Sbjct: 541 GGWTAIRFRADNPGVWFMHCHLEVHTGWGLKTAFVVEDGPGKDQSILPPPKDLPPC 563

BLAST of Lsi07G002590 vs. NCBI nr
Match: XP_038905726.1 (laccase-11-like [Benincasa hispida])

HSP 1 Score: 1164.1 bits (3010), Expect = 0.0e+00
Identity = 558/596 (93.62%), Postives = 568/596 (95.30%), Query Frame = 0

Query: 4   MASLVWFVHRLTLLFVACCLGLISFSAEAAIKRYQFDVQVQNISRLCHAKPIVTVNGKFP 63
           MA LVWF+HRLT LFVACCLGLISFSAEAAIKRYQFDVQVQNISRLCHAKPIVTVNGKFP
Sbjct: 1   MARLVWFIHRLTFLFVACCLGLISFSAEAAIKRYQFDVQVQNISRLCHAKPIVTVNGKFP 60

Query: 64  GPTIYVQEGDRVLVNVTNHAQYNMTLMTRVKIEPPPTPQYMCVFIYVTWVLISVAFCIFC 123
           GPTIYVQEGDRVLVNVTNHAQYNM+       +             +TW  ISVAFCIFC
Sbjct: 61  GPTIYVQEGDRVLVNVTNHAQYNMSXXXXXXKK------------NITW-FISVAFCIFC 120

Query: 124 RHGLKQYRNGWADGPAYITQCPIQRGNSYTYDFNVTGQRGTLWWHAHILWLRATVYGAIV 183
           RHGLKQYRNGWADGPAYITQCPIQRGNSYTYDFNVTGQRGTLWWHAHILWLRATVYGAIV
Sbjct: 121 RHGLKQYRNGWADGPAYITQCPIQRGNSYTYDFNVTGQRGTLWWHAHILWLRATVYGAIV 180

Query: 184 IMPKQGTPYPFPQPNREFEILLGEWWNNDVEAVVNQGSSMGVPPNMSDAHTINGKSGPLF 243
           I+PKQGTPYPFPQPNREFEILLGEWWNNDVEAVVNQGSSMGVPPNMSDAHTINGK GPLF
Sbjct: 181 ILPKQGTPYPFPQPNREFEILLGEWWNNDVEAVVNQGSSMGVPPNMSDAHTINGKPGPLF 240

Query: 244 PCSEKHTFAMEVETGKTYLLRIINAALNDELFFAIAGHNLTVVEVDAVYTKSFSTQAILI 303
           PCSEKHTFAMEVE GKTYLLRIINAALNDELFFAIAGHN+TVVEVDAVYTK F+TQAILI
Sbjct: 241 PCSEKHTFAMEVEAGKTYLLRIINAALNDELFFAIAGHNMTVVEVDAVYTKPFTTQAILI 300

Query: 304 APGQTTNVLLQANQAPNRYFMASRSFMDVPIPVDNKTATGILQYKGIPNTILPILPQLPA 363
           APGQTTNVLLQANQAPNRYFMASRSFMDVPIPVDNKTATGILQYKGIPNTILPILPQLPA
Sbjct: 301 APGQTTNVLLQANQAPNRYFMASRSFMDVPIPVDNKTATGILQYKGIPNTILPILPQLPA 360

Query: 364 SNDTSFALNYNKKLKSLNSPQYPANVPLKVDRKLFYTIGLGQNACPTCLNGTQFVASLNN 423
           SNDTSFAL+YNKKLKSLNSPQYPANVPLKVDRKLFYTIGLGQNACPTCLNGTQ VASLNN
Sbjct: 361 SNDTSFALSYNKKLKSLNSPQYPANVPLKVDRKLFYTIGLGQNACPTCLNGTQLVASLNN 420

Query: 424 ISFVMPQIGLLQSHYFNISGVFRTDFPDRPPTPFNYTGAPLTANLRTAVGTRLSKIAFNS 483
           ISFVMPQIGLLQSHYFNISGVFRTDFPD+PPTPFNYTGAPLTANLRTAVGTRLSKIAFNS
Sbjct: 421 ISFVMPQIGLLQSHYFNISGVFRTDFPDKPPTPFNYTGAPLTANLRTAVGTRLSKIAFNS 480

Query: 484 TVELVLQDTNLLTVESHPFHLHGYNFFVVGTGIGNFNPAKDPAKYNLVDPVERNTVGVPT 543
           TVELVLQDTNLLTVESHPFHLHGYNFFV+GTGIGNFNPAKDPAKYNLVDPVERNTVGVPT
Sbjct: 481 TVELVLQDTNLLTVESHPFHLHGYNFFVIGTGIGNFNPAKDPAKYNLVDPVERNTVGVPT 540

Query: 544 GGWTAIRFRADNPGVWFMHCHLEVHTGWGLKTAFVVEDGPGKDQTILPPPKDLPPC 600
           GGWTAIRFRADNPGVWFMHCHLEVHTGWGLKTAFVVEDGPGKDQTILPPPKDLPPC
Sbjct: 541 GGWTAIRFRADNPGVWFMHCHLEVHTGWGLKTAFVVEDGPGKDQTILPPPKDLPPC 583

BLAST of Lsi07G002590 vs. NCBI nr
Match: XP_008452071.1 (PREDICTED: laccase-11-like [Cucumis melo] >TYK16659.1 laccase-11-like [Cucumis melo var. makuwa])

HSP 1 Score: 1118.2 bits (2891), Expect = 0.0e+00
Identity = 540/596 (90.60%), Postives = 549/596 (92.11%), Query Frame = 0

Query: 4   MASLVWFVHRLTLLFVACCLGLISFSAEAAIKRYQFDVQVQNISRLCHAKPIVTVNGKFP 63
           MA LVW VHRLT LFVA CLG ISFS EAAIKRYQFDVQVQNISRLCHAKPIVTVNGKFP
Sbjct: 1   MAGLVWVVHRLTFLFVAYCLGFISFSVEAAIKRYQFDVQVQNISRLCHAKPIVTVNGKFP 60

Query: 64  GPTIYVQEGDRVLVNVTNHAQYNMTLMTRVKIEPPPTPQYMCVFIYVTWVLISVAFCIFC 123
           GPTIYVQEGDRVLVNVTNHAQYNM+                     + W           
Sbjct: 61  GPTIYVQEGDRVLVNVTNHAQYNMS---------------------IHW----------- 120

Query: 124 RHGLKQYRNGWADGPAYITQCPIQRGNSYTYDFNVTGQRGTLWWHAHILWLRATVYGAIV 183
            HGLKQYRNGWADGPAYITQCPIQRGNSYTYDFNVTGQRGTLWWHAHILWLRATVYGAIV
Sbjct: 121 -HGLKQYRNGWADGPAYITQCPIQRGNSYTYDFNVTGQRGTLWWHAHILWLRATVYGAIV 180

Query: 184 IMPKQGTPYPFPQPNREFEILLGEWWNNDVEAVVNQGSSMGVPPNMSDAHTINGKSGPLF 243
           I+PKQGTPYPFPQPN EFEILLGEWWNNDVEAVVNQGS MGVPPNMSDAHTINGK GPLF
Sbjct: 181 ILPKQGTPYPFPQPNGEFEILLGEWWNNDVEAVVNQGSRMGVPPNMSDAHTINGKPGPLF 240

Query: 244 PCSEKHTFAMEVETGKTYLLRIINAALNDELFFAIAGHNLTVVEVDAVYTKSFSTQAILI 303
           PCSEKHTFAMEVE GKTYLLRIINAALNDELFFAIAGHN+TVVEVDAVYTK F+TQAILI
Sbjct: 241 PCSEKHTFAMEVEAGKTYLLRIINAALNDELFFAIAGHNMTVVEVDAVYTKPFTTQAILI 300

Query: 304 APGQTTNVLLQANQAPNRYFMASRSFMDVPIPVDNKTATGILQYKGIPNTILPILPQLPA 363
           APGQTTNVLLQANQAPNRYFMASRSFMDVPIPVDNKTATGILQY+GIPNTILPILPQLPA
Sbjct: 301 APGQTTNVLLQANQAPNRYFMASRSFMDVPIPVDNKTATGILQYRGIPNTILPILPQLPA 360

Query: 364 SNDTSFALNYNKKLKSLNSPQYPANVPLKVDRKLFYTIGLGQNACPTCLNGTQFVASLNN 423
           SNDTSFAL+YNKKLKSLNSPQYPANVPL+VDRKLFYTIGLGQN CPTCLNGTQ VASLNN
Sbjct: 361 SNDTSFALSYNKKLKSLNSPQYPANVPLEVDRKLFYTIGLGQNDCPTCLNGTQLVASLNN 420

Query: 424 ISFVMPQIGLLQSHYFNISGVFRTDFPDRPPTPFNYTGAPLTANLRTAVGTRLSKIAFNS 483
           ISFVMPQIGLLQSHYFNISGVFRTDFPDRPPTPFNYTGAPLTANLRTAVGTRLSKIAFNS
Sbjct: 421 ISFVMPQIGLLQSHYFNISGVFRTDFPDRPPTPFNYTGAPLTANLRTAVGTRLSKIAFNS 480

Query: 484 TVELVLQDTNLLTVESHPFHLHGYNFFVVGTGIGNFNPAKDPAKYNLVDPVERNTVGVPT 543
           TVELVLQDTNLLTVESHPFHLHGYNFFVVGTGIGNFNPAKDPAKYNLVDPVERNTVGVPT
Sbjct: 481 TVELVLQDTNLLTVESHPFHLHGYNFFVVGTGIGNFNPAKDPAKYNLVDPVERNTVGVPT 540

Query: 544 GGWTAIRFRADNPGVWFMHCHLEVHTGWGLKTAFVVEDGPGKDQTILPPPKDLPPC 600
           GGWTAIRFRADNPGVWFMHCHLEVHTGWGLKTAFVVEDGPGKDQ+ILPPPKDLPPC
Sbjct: 541 GGWTAIRFRADNPGVWFMHCHLEVHTGWGLKTAFVVEDGPGKDQSILPPPKDLPPC 563

BLAST of Lsi07G002590 vs. NCBI nr
Match: XP_023552611.1 (laccase-11-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1117.8 bits (2890), Expect = 0.0e+00
Identity = 535/598 (89.46%), Postives = 555/598 (92.81%), Query Frame = 0

Query: 2   LTMASLVWFVHRLTLLFVACCLGLISFSAEAAIKRYQFDVQVQNISRLCHAKPIVTVNGK 61
           +TMASLV FV RLT LFVACC GLISFSAEAA+KRYQFDVQVQNISRLCHAKPIVT+NG+
Sbjct: 1   MTMASLVCFVRRLTFLFVACCFGLISFSAEAAVKRYQFDVQVQNISRLCHAKPIVTINGR 60

Query: 62  FPGPTIYVQEGDRVLVNVTNHAQYNMTLMTRVKIEPPPTPQYMCVFIYVTWVLISVAFCI 121
           FPGPTIYVQEGDRVLVNVTNHAQYNM+                     + W         
Sbjct: 61  FPGPTIYVQEGDRVLVNVTNHAQYNMS---------------------IHW--------- 120

Query: 122 FCRHGLKQYRNGWADGPAYITQCPIQRGNSYTYDFNVTGQRGTLWWHAHILWLRATVYGA 181
              HGLKQYRNGWADGPAYITQCPIQRG+SYTYDFNVTGQRGTLWWHAHILWLRATVYGA
Sbjct: 121 ---HGLKQYRNGWADGPAYITQCPIQRGSSYTYDFNVTGQRGTLWWHAHILWLRATVYGA 180

Query: 182 IVIMPKQGTPYPFPQPNREFEILLGEWWNNDVEAVVNQGSSMGVPPNMSDAHTINGKSGP 241
           IVI+PKQGTPYPFPQPNREFEILLGEWWNNDVEA+VNQGSSMGVPPNMSDAHTINGK GP
Sbjct: 181 IVILPKQGTPYPFPQPNREFEILLGEWWNNDVEAIVNQGSSMGVPPNMSDAHTINGKPGP 240

Query: 242 LFPCSEKHTFAMEVETGKTYLLRIINAALNDELFFAIAGHNLTVVEVDAVYTKSFSTQAI 301
           LFPCSEKHTFAMEVE GKTYLLRIINAALNDELFFAIAGHN+TVVEVDAVYTK F+TQAI
Sbjct: 241 LFPCSEKHTFAMEVEAGKTYLLRIINAALNDELFFAIAGHNMTVVEVDAVYTKPFTTQAI 300

Query: 302 LIAPGQTTNVLLQANQAPNRYFMASRSFMDVPIPVDNKTATGILQYKGIPNTILPILPQL 361
           LIAPGQTTNVL+QANQ PNRYFMASRSFMDVPIPVDNKTATGILQYKGIPNTILP+LPQL
Sbjct: 301 LIAPGQTTNVLVQANQPPNRYFMASRSFMDVPIPVDNKTATGILQYKGIPNTILPVLPQL 360

Query: 362 PASNDTSFALNYNKKLKSLNSPQYPANVPLKVDRKLFYTIGLGQNACPTCLNGTQFVASL 421
           PASND+SFAL+YNKK+KSLN+PQYPANVPLKVDRKLFYTIGLGQNACPTC+NGTQ VASL
Sbjct: 361 PASNDSSFALSYNKKIKSLNTPQYPANVPLKVDRKLFYTIGLGQNACPTCVNGTQLVASL 420

Query: 422 NNISFVMPQIGLLQSHYFNISGVFRTDFPDRPPTPFNYTGAPLTANLRTAVGTRLSKIAF 481
           NNISFVMPQIGLLQSHYFNISGVFRTDFPDRPPTPFNYTGAPLTANLRTAVGTRLSKIAF
Sbjct: 421 NNISFVMPQIGLLQSHYFNISGVFRTDFPDRPPTPFNYTGAPLTANLRTAVGTRLSKIAF 480

Query: 482 NSTVELVLQDTNLLTVESHPFHLHGYNFFVVGTGIGNFNPAKDPAKYNLVDPVERNTVGV 541
           NSTVELVLQDTNLLTVESHPFHLHGYNFFVVGTGIGNF+PAKDPAKYNLVDPVERNTVGV
Sbjct: 481 NSTVELVLQDTNLLTVESHPFHLHGYNFFVVGTGIGNFDPAKDPAKYNLVDPVERNTVGV 540

Query: 542 PTGGWTAIRFRADNPGVWFMHCHLEVHTGWGLKTAFVVEDGPGKDQTILPPPKDLPPC 600
           PTGGWTAIRFRADNPGVWFMHCHLEVHTGWGLKTAFVVEDGPGKDQ+ILPPPKDLPPC
Sbjct: 541 PTGGWTAIRFRADNPGVWFMHCHLEVHTGWGLKTAFVVEDGPGKDQSILPPPKDLPPC 565

BLAST of Lsi07G002590 vs. NCBI nr
Match: XP_004146556.1 (laccase-11 [Cucumis sativus] >KGN53314.1 hypothetical protein Csa_015210 [Cucumis sativus])

HSP 1 Score: 1116.7 bits (2887), Expect = 0.0e+00
Identity = 537/596 (90.10%), Postives = 550/596 (92.28%), Query Frame = 0

Query: 4   MASLVWFVHRLTLLFVACCLGLISFSAEAAIKRYQFDVQVQNISRLCHAKPIVTVNGKFP 63
           MASLVWFVHRLT LFVA C+GLISFS EAAIKRYQFDVQVQNISRLCHAKPIVTVNGKFP
Sbjct: 1   MASLVWFVHRLTFLFVASCIGLISFSVEAAIKRYQFDVQVQNISRLCHAKPIVTVNGKFP 60

Query: 64  GPTIYVQEGDRVLVNVTNHAQYNMTLMTRVKIEPPPTPQYMCVFIYVTWVLISVAFCIFC 123
           GPTIYVQEGDRVLVNVTNHAQYNM+                     + W           
Sbjct: 61  GPTIYVQEGDRVLVNVTNHAQYNMS---------------------IHW----------- 120

Query: 124 RHGLKQYRNGWADGPAYITQCPIQRGNSYTYDFNVTGQRGTLWWHAHILWLRATVYGAIV 183
            HGLKQYRNGWADGPAYITQCPIQRGNSYTYDFNVTGQRGTLWWHAHILWLRATVYGAIV
Sbjct: 121 -HGLKQYRNGWADGPAYITQCPIQRGNSYTYDFNVTGQRGTLWWHAHILWLRATVYGAIV 180

Query: 184 IMPKQGTPYPFPQPNREFEILLGEWWNNDVEAVVNQGSSMGVPPNMSDAHTINGKSGPLF 243
           I+PKQGTPYPFPQPN EFEILLGEWWNNDVEAVVNQGS MG+PPNMSDAHTINGK GPLF
Sbjct: 181 ILPKQGTPYPFPQPNGEFEILLGEWWNNDVEAVVNQGSRMGLPPNMSDAHTINGKPGPLF 240

Query: 244 PCSEKHTFAMEVETGKTYLLRIINAALNDELFFAIAGHNLTVVEVDAVYTKSFSTQAILI 303
           PCSEKHTFAMEVE GKTYLLRIINAALNDELFFAIAGH++TVVEVDAVYTK F+TQAILI
Sbjct: 241 PCSEKHTFAMEVEAGKTYLLRIINAALNDELFFAIAGHSMTVVEVDAVYTKPFTTQAILI 300

Query: 304 APGQTTNVLLQANQAPNRYFMASRSFMDVPIPVDNKTATGILQYKGIPNTILPILPQLPA 363
           APGQTTNVLLQANQAPNRYFMASRSFMDVPIPVDNKTATGILQY+GIPNT+LP LPQLPA
Sbjct: 301 APGQTTNVLLQANQAPNRYFMASRSFMDVPIPVDNKTATGILQYRGIPNTVLPTLPQLPA 360

Query: 364 SNDTSFALNYNKKLKSLNSPQYPANVPLKVDRKLFYTIGLGQNACPTCLNGTQFVASLNN 423
           SNDTSFAL+YNKKLKSLNSPQYPANVPLKVDRKLFYTIGLGQN CPTCLNGTQ VASLNN
Sbjct: 361 SNDTSFALSYNKKLKSLNSPQYPANVPLKVDRKLFYTIGLGQNECPTCLNGTQLVASLNN 420

Query: 424 ISFVMPQIGLLQSHYFNISGVFRTDFPDRPPTPFNYTGAPLTANLRTAVGTRLSKIAFNS 483
           ISFVMPQIGLLQSHYFNI GVFRTDFPDRPPTPFNYTGAPLTANLRTAVGTRLSKIAFNS
Sbjct: 421 ISFVMPQIGLLQSHYFNIGGVFRTDFPDRPPTPFNYTGAPLTANLRTAVGTRLSKIAFNS 480

Query: 484 TVELVLQDTNLLTVESHPFHLHGYNFFVVGTGIGNFNPAKDPAKYNLVDPVERNTVGVPT 543
           TVELVLQDTNLLTVESHPFHLHGYNFFVVGTGIGNF+PAKDPAKYNLVDPVERNTVGVPT
Sbjct: 481 TVELVLQDTNLLTVESHPFHLHGYNFFVVGTGIGNFDPAKDPAKYNLVDPVERNTVGVPT 540

Query: 544 GGWTAIRFRADNPGVWFMHCHLEVHTGWGLKTAFVVEDGPGKDQTILPPPKDLPPC 600
           GGWTAIRFRADNPGVWFMHCHLEVHTGWGLKTAFVVEDGPGKDQ+ILPPPKDLPPC
Sbjct: 541 GGWTAIRFRADNPGVWFMHCHLEVHTGWGLKTAFVVEDGPGKDQSILPPPKDLPPC 563

BLAST of Lsi07G002590 vs. NCBI nr
Match: XP_022929401.1 (laccase-11-like [Cucurbita moschata])

HSP 1 Score: 1113.2 bits (2878), Expect = 0.0e+00
Identity = 532/598 (88.96%), Postives = 552/598 (92.31%), Query Frame = 0

Query: 2   LTMASLVWFVHRLTLLFVACCLGLISFSAEAAIKRYQFDVQVQNISRLCHAKPIVTVNGK 61
           +TMASLV FV RLT +FVACC GLISFS EAAIKRYQFDVQVQNISRLCHAKPIVT+NG+
Sbjct: 1   MTMASLVCFVRRLTFVFVACCFGLISFSVEAAIKRYQFDVQVQNISRLCHAKPIVTINGR 60

Query: 62  FPGPTIYVQEGDRVLVNVTNHAQYNMTLMTRVKIEPPPTPQYMCVFIYVTWVLISVAFCI 121
           FPGPTIYVQEGDRVLVNVTNHAQYNM+                     + W         
Sbjct: 61  FPGPTIYVQEGDRVLVNVTNHAQYNMS---------------------IHW--------- 120

Query: 122 FCRHGLKQYRNGWADGPAYITQCPIQRGNSYTYDFNVTGQRGTLWWHAHILWLRATVYGA 181
              HGLKQYRNGWADGPAYITQCPIQRG+SYTYDFNVTGQRGTLWWHAHILWLRATVYGA
Sbjct: 121 ---HGLKQYRNGWADGPAYITQCPIQRGSSYTYDFNVTGQRGTLWWHAHILWLRATVYGA 180

Query: 182 IVIMPKQGTPYPFPQPNREFEILLGEWWNNDVEAVVNQGSSMGVPPNMSDAHTINGKSGP 241
           IVI+PKQGTPYPFPQPNREFEILLGEWWNNDVEA+VNQGSSMGVPPNMSDAHTINGK GP
Sbjct: 181 IVILPKQGTPYPFPQPNREFEILLGEWWNNDVEAIVNQGSSMGVPPNMSDAHTINGKPGP 240

Query: 242 LFPCSEKHTFAMEVETGKTYLLRIINAALNDELFFAIAGHNLTVVEVDAVYTKSFSTQAI 301
           LFPCSEKHTFAMEVE GKTYLLRIINAALNDELFFAIAGHN+TVVEVDAVYTK F+TQAI
Sbjct: 241 LFPCSEKHTFAMEVEAGKTYLLRIINAALNDELFFAIAGHNMTVVEVDAVYTKPFTTQAI 300

Query: 302 LIAPGQTTNVLLQANQAPNRYFMASRSFMDVPIPVDNKTATGILQYKGIPNTILPILPQL 361
           LIAPGQTTNVL+QANQ PNRYFMASRSFMDVPIPVDNKTATGILQYKGIPNTILP+LPQL
Sbjct: 301 LIAPGQTTNVLVQANQPPNRYFMASRSFMDVPIPVDNKTATGILQYKGIPNTILPVLPQL 360

Query: 362 PASNDTSFALNYNKKLKSLNSPQYPANVPLKVDRKLFYTIGLGQNACPTCLNGTQFVASL 421
           PASND+SFAL+YNKK+KSLNSPQYPANVPLKVDRKLFYTIGLGQNACPTC+NGTQ VASL
Sbjct: 361 PASNDSSFALSYNKKIKSLNSPQYPANVPLKVDRKLFYTIGLGQNACPTCINGTQLVASL 420

Query: 422 NNISFVMPQIGLLQSHYFNISGVFRTDFPDRPPTPFNYTGAPLTANLRTAVGTRLSKIAF 481
           NNISFVMPQIGLLQSHYFNISGVFR DFPDRPPTPFNYTGAPLTANLRTAVGTRL KIAF
Sbjct: 421 NNISFVMPQIGLLQSHYFNISGVFRADFPDRPPTPFNYTGAPLTANLRTAVGTRLGKIAF 480

Query: 482 NSTVELVLQDTNLLTVESHPFHLHGYNFFVVGTGIGNFNPAKDPAKYNLVDPVERNTVGV 541
           NSTVELVLQDTNLLTVESHPFHLHGYNFFVVGTGIGNF+PAKDPAKYNLVDP+ERNTVGV
Sbjct: 481 NSTVELVLQDTNLLTVESHPFHLHGYNFFVVGTGIGNFDPAKDPAKYNLVDPIERNTVGV 540

Query: 542 PTGGWTAIRFRADNPGVWFMHCHLEVHTGWGLKTAFVVEDGPGKDQTILPPPKDLPPC 600
           PTGGWTAIRFRADNPGVWFMHCHLEVHTGWGLKTAFVVEDGPGKDQ+ILPPPKDLPPC
Sbjct: 541 PTGGWTAIRFRADNPGVWFMHCHLEVHTGWGLKTAFVVEDGPGKDQSILPPPKDLPPC 565

BLAST of Lsi07G002590 vs. TAIR 10
Match: AT5G03260.1 (laccase 11 )

HSP 1 Score: 908.7 bits (2347), Expect = 2.5e-264
Identity = 422/589 (71.65%), Postives = 483/589 (82.00%), Query Frame = 0

Query: 13  RLTLLFVAC-CLGLISFS-AEAAIKRYQFDVQVQNISRLCHAKPIVTVNGKFPGPTIYVQ 72
           ++  LF+ C  L  + +S  +AA+K+YQFDVQV+NISR+C+AKPIVTVNG FPGPT+Y +
Sbjct: 2   KMGFLFLFCYLLAFLGYSPVDAAVKKYQFDVQVKNISRICNAKPIVTVNGMFPGPTVYAR 61

Query: 73  EGDRVLVNVTNHAQYNMTLMTRVKIEPPPTPQYMCVFIYVTWVLISVAFCIFCRHGLKQY 132
           EGDRV++NVTNH QYNM+                     + W            HGLKQY
Sbjct: 62  EGDRVIINVTNHVQYNMS---------------------IHW------------HGLKQY 121

Query: 133 RNGWADGPAYITQCPIQRGNSYTYDFNVTGQRGTLWWHAHILWLRATVYGAIVIMPKQGT 192
           RNGWADGPAYITQCPIQ G SY YDFNVTGQRGTLWWHAHILWLRATVYGAIVI+P  G 
Sbjct: 122 RNGWADGPAYITQCPIQTGQSYLYDFNVTGQRGTLWWHAHILWLRATVYGAIVILPAPGK 181

Query: 193 PYPFPQPNREFEILLGEWWNNDVEAVVNQGSSMGVPPNMSDAHTINGKSGPLFPCSEKHT 252
           PYPFPQP +E  I+LGEWWN DVE  VNQ + +G PP MSDAHTINGK GPLFPCSEKHT
Sbjct: 182 PYPFPQPYQESNIILGEWWNKDVETAVNQANQLGAPPPMSDAHTINGKPGPLFPCSEKHT 241

Query: 253 FAMEVETGKTYLLRIINAALNDELFFAIAGHNLTVVEVDAVYTKSFSTQAILIAPGQTTN 312
           F +E E GKTYLLRIINAALNDELFF IAGHN+TVVE+DAVYTK F+T+AIL+ PGQTTN
Sbjct: 242 FVIEAEAGKTYLLRIINAALNDELFFGIAGHNMTVVEIDAVYTKPFTTKAILLGPGQTTN 301

Query: 313 VLLQANQAPNRYFMASRSFMDVPIPVDNKTATGILQYKGIPNTILPILPQLPASNDTSFA 372
           VL++ +++PNRYFMA+  FMD P+ VDNKT T ILQYKG+PNT+LPILP+LP  NDTSFA
Sbjct: 302 VLVKTDRSPNRYFMAASPFMDAPVSVDNKTVTAILQYKGVPNTVLPILPKLPLPNDTSFA 361

Query: 373 LNYNKKLKSLNSPQYPANVPLKVDRKLFYTIGLGQNACPTCLNGTQFVASLNNISFVMPQ 432
           L+YN KLKSLN+P +PA VPLKVDR+LFYTIGLG NACPTC+NGT   AS+NNI+F+MP+
Sbjct: 362 LDYNGKLKSLNTPNFPALVPLKVDRRLFYTIGLGINACPTCVNGTNLAASINNITFIMPK 421

Query: 433 IGLLQSHYFNISGVFRTDFPDRPPTPFNYTGAPLTANLRTAVGTRLSKIAFNSTVELVLQ 492
             LL++HY NISGVFRTDFPDRPP  FNYTG PLTANL T+ GTRLS++ FN+T+ELVLQ
Sbjct: 422 TALLKAHYSNISGVFRTDFPDRPPKAFNYTGVPLTANLGTSTGTRLSRVKFNTTIELVLQ 481

Query: 493 DTNLLTVESHPFHLHGYNFFVVGTGIGNFNPAKDPAKYNLVDPVERNTVGVPTGGWTAIR 552
           DTNLLTVESHPFHLHGYNFFVVGTG+GNF+P KDPAK+NLVDP ERNTVGVPTGGW AIR
Sbjct: 482 DTNLLTVESHPFHLHGYNFFVVGTGVGNFDPKKDPAKFNLVDPPERNTVGVPTGGWAAIR 541

Query: 553 FRADNPGVWFMHCHLEVHTGWGLKTAFVVEDGPGKDQTILPPPKDLPPC 600
           FRADNPGVWFMHCHLEVHT WGLK AFVVE+G   + ++LPPPKD P C
Sbjct: 542 FRADNPGVWFMHCHLEVHTMWGLKMAFVVENGETPELSVLPPPKDYPSC 557

BLAST of Lsi07G002590 vs. TAIR 10
Match: AT2G38080.1 (Laccase/Diphenol oxidase family protein )

HSP 1 Score: 708.4 bits (1827), Expect = 5.0e-204
Identity = 328/590 (55.59%), Postives = 420/590 (71.19%), Query Frame = 0

Query: 12  HRLTLLFVACCLGLISFSAEAAIKRYQFDVQVQNISRLCHAKPIVTVNGKFPGPTIYVQE 71
           H +  LF+     +    +E+ ++ Y+F+V ++N++RLC +KP VTVNG++PGPTIY +E
Sbjct: 4   HMVWFLFLVSFFSVFPAPSESMVRHYKFNVVMKNVTRLCSSKPTVTVNGRYPGPTIYARE 63

Query: 72  GDRVLVNVTNHAQYNMTLMTRVKIEPPPTPQYMCVFIYVTWVLISVAFCIFCRHGLKQYR 131
            D +L+ V NH +YN++                     + W            HG++Q R
Sbjct: 64  DDTLLIKVVNHVKYNVS---------------------IHW------------HGVRQVR 123

Query: 132 NGWADGPAYITQCPIQRGNSYTYDFNVTGQRGTLWWHAHILWLRATVYGAIVIMPKQGTP 191
            GWADGPAYITQCPIQ G  YTY++ +TGQRGTLWWHAHILWLRATVYGA+VI+PK+G P
Sbjct: 124 TGWADGPAYITQCPIQPGQVYTYNYTLTGQRGTLWWHAHILWLRATVYGALVILPKRGVP 183

Query: 192 YPFPQPNREFEILLGEWWNNDVEAVVNQGSSMGVPPNMSDAHTINGKSGPLFPCSEKHTF 251
           YPFP+P+ E  I+LGEWW +D E ++N+    G+ PN+SD+H ING  GP+  C  +  +
Sbjct: 184 YPFPKPDNEKVIVLGEWWKSDTENIINEALKSGLAPNVSDSHMINGHPGPVRNCPSQ-GY 243

Query: 252 AMEVETGKTYLLRIINAALNDELFFAIAGHNLTVVEVDAVYTKSFSTQAILIAPGQTTNV 311
            + VE GKTYLLR++NAALN+ELFF +AGH  TVVEVDAVY K F T  +LIAPGQTTNV
Sbjct: 244 KLSVENGKTYLLRLVNAALNEELFFKVAGHIFTVVEVDAVYVKPFKTDTVLIAPGQTTNV 303

Query: 312 LLQANQAPNRYFMASRSFMDVPIPVDNKTATGILQYKGIPNTILPILPQLPASNDTSFAL 371
           LL A+++  +Y + +  FMD PI VDN TAT  + Y G  ++   IL   P  N TS A 
Sbjct: 304 LLTASKSAGKYLVTASPFMDAPIAVDNVTATATVHYSGTLSSSPTILTLPPPQNATSIAN 363

Query: 372 NYNKKLKSLNSPQYPANVPLKVDRKLFYTIGLGQNACPTCL--NGTQFVASLNNISFVMP 431
           N+   L+SLNS +YPA VP  +D  LF+T+GLG NACPTC   NG++ VAS+NN++F+MP
Sbjct: 364 NFTNSLRSLNSKKYPALVPTTIDHHLFFTVGLGLNACPTCKAGNGSRVVASINNVTFIMP 423

Query: 432 QIGLLQSHYFNISGVFRTDFPDRPPTPFNYTGAPLTANLRTAVGTRLSKIAFNSTVELVL 491
           +  LL +HYFN SGVF TDFP  PP  FNY+G  +T N+ T  GTRL K+ +N+TV+LVL
Sbjct: 424 KTALLPAHYFNTSGVFTTDFPKNPPHVFNYSGGSVT-NMATETGTRLYKLPYNATVQLVL 483

Query: 492 QDTNLLTVESHPFHLHGYNFFVVGTGIGNFNPAKDPAKYNLVDPVERNTVGVPTGGWTAI 551
           QDT ++  E+HP HLHG+NFF VG G+GNFN  KDP  +NLVDPVERNT+GVP+GGW  I
Sbjct: 484 QDTGVIAPENHPVHLHGFNFFEVGRGLGNFNSTKDPKNFNLVDPVERNTIGVPSGGWVVI 543

Query: 552 RFRADNPGVWFMHCHLEVHTGWGLKTAFVVEDGPGKDQTILPPPKDLPPC 600
           RFRADNPGVWFMHCHLEVHT WGLK AF+VE+G G +Q+ILPPPKDLP C
Sbjct: 544 RFRADNPGVWFMHCHLEVHTTWGLKMAFLVENGKGPNQSILPPPKDLPKC 558

BLAST of Lsi07G002590 vs. TAIR 10
Match: AT5G01190.1 (laccase 10 )

HSP 1 Score: 665.2 bits (1715), Expect = 4.8e-191
Identity = 324/595 (54.45%), Postives = 409/595 (68.74%), Query Frame = 0

Query: 10  FVHRLTLLFVACCLGLISFSA--EAAIKRYQFDVQVQNISRLCHAKPIVTVNGKFPGPTI 69
           F  R+ +LF      L++F A    AI++Y F+V  + ++R+C  K IVTVNGKFPGPTI
Sbjct: 3   FPIRILVLF-----ALLAFPACVHGAIRKYTFNVVTKQVTRICSTKQIVTVNGKFPGPTI 62

Query: 70  YVQEGDRVLVNVTNHAQYNMTLMTRVKIEPPPTPQYMCVFIYVTWVLISVAFCIFCRHGL 129
           Y  E D +LVNV N+ +YN++                     + W            HG+
Sbjct: 63  YANEDDTILVNVVNNVKYNVS---------------------IHW------------HGI 122

Query: 130 KQYRNGWADGPAYITQCPIQRGNSYTYDFNVTGQRGTLWWHAHILWLRATVYGAIVIMPK 189
           +Q R GWADGPAYITQCPI+ G+SY Y+F VTGQRGTLWWHAH+LWLRATV+GAIVI+PK
Sbjct: 123 RQLRTGWADGPAYITQCPIKPGHSYVYNFTVTGQRGTLWWHAHVLWLRATVHGAIVILPK 182

Query: 190 QGTPYPFPQPNREFEILLGEWWNNDVEAVVNQGSSMGVPPNMSDAHTINGKSGPLFPCSE 249
            G PYPFP+P+RE  I+LGEWW +D E VVN+    G+ PN+SDAH ING  G +  C  
Sbjct: 183 LGLPYPFPKPHREEVIILGEWWKSDTETVVNEALKSGLAPNVSDAHVINGHPGFVPNCPS 242

Query: 250 KHTFAMEVETGKTYLLRIINAALNDELFFAIAGHNLTVVEVDAVYTKSFSTQAILIAPGQ 309
           +  F + VE+GKTY+LR+INAALN+ELFF IAGH  TVVEVDAVY K F+T  ILIAPGQ
Sbjct: 243 QGNFKLAVESGKTYMLRLINAALNEELFFKIAGHRFTVVEVDAVYVKPFNTDTILIAPGQ 302

Query: 310 TTNVLLQANQAPNRYFMASRSFMD-VPIPVDNKTATGILQYKGIPNTILPILPQLPASND 369
           TT  L+ A +   +Y +A+  F D   + VDN+TAT  + Y G  +         P  N 
Sbjct: 303 TTTALVSAARPSGQYLIAAAPFQDSAVVAVDNRTATATVHYSGTLSATPTKTTSPPPQNA 362

Query: 370 TSFALNYNKKLKSLNSPQYPANVPLKVDRKLFYTIGLGQNACPTCLNG--TQFVASLNNI 429
           TS A  +   L+SLNS  YPANVP+ VD  L +T+GLG N C +C  G  ++ VA++NNI
Sbjct: 363 TSVANTFVNSLRSLNSKTYPANVPITVDHDLLFTVGLGINRCHSCKAGNFSRVVAAINNI 422

Query: 430 SFVMPQIGLLQSHYFNISGVFRTDFPDRPPTPFNYTGAPLTANLRTAVGTRLSKIAFNST 489
           +F MP+  LLQ+HYFN++G++ TDFP +P   F++TG P  +NL T   T+L K+ +NST
Sbjct: 423 TFKMPKTALLQAHYFNLTGIYTTDFPAKPRRVFDFTGKP-PSNLATMKATKLYKLPYNST 482

Query: 490 VELVLQDTNLLTVESHPFHLHGYNFFVVGTGIGNFNPAKDPAKYNLVDPVERNTVGVPTG 549
           V++VLQDT  +  E+HP HLHG+NFFVVG G GN+N  KD  K+NLVDPVERNTVGVP+G
Sbjct: 483 VQVVLQDTGNVAPENHPIHLHGFNFFVVGLGTGNYNSKKDSNKFNLVDPVERNTVGVPSG 542

Query: 550 GWTAIRFRADNPGVWFMHCHLEVHTGWGLKTAFVVEDGPGKDQTILPPPKDLPPC 600
           GW AIRFRADNPGVWFMHCHLEVHT WGLK AF+VE+G G +Q+I PPP DLP C
Sbjct: 543 GWAAIRFRADNPGVWFMHCHLEVHTTWGLKMAFLVENGKGPNQSIRPPPSDLPKC 558

BLAST of Lsi07G002590 vs. TAIR 10
Match: AT5G58910.1 (laccase 16 )

HSP 1 Score: 661.8 bits (1706), Expect = 5.4e-190
Identity = 311/561 (55.44%), Postives = 392/561 (69.88%), Query Frame = 0

Query: 45  NISRLCHAKPIVTVNGKFPGPTIYVQEGDRVLVNVTNHAQYNMTLMTRVKIEPPPTPQYM 104
           N ++LC +KPIVTVNG+FPGPTI  +EGD +L+ V NH +YN+++               
Sbjct: 3   NTTKLCSSKPIVTVNGQFPGPTIVAREGDTILIKVVNHVKYNVSI--------------- 62

Query: 105 CVFIYVTWVLISVAFCIFCRHGLKQYRNGWADGPAYITQCPIQRGNSYTYDFNVTGQRGT 164
                                    +  GWADGPAYITQCPIQ G +Y ++F +TGQRGT
Sbjct: 63  -------------------------HWTGWADGPAYITQCPIQPGQNYLHNFTLTGQRGT 122

Query: 165 LWWHAHILWLRATVYGAIVIMPKQGTPYPFPQPNREFEILLGEWWNNDVEAVVNQGSSMG 224
           LWWHAHILWLRATV+GAIVI+PK G PYPFP+P +E  I+L EWW +DVE ++N+ S +G
Sbjct: 123 LWWHAHILWLRATVHGAIVILPKLGVPYPFPKPYKEKTIVLSEWWKSDVEELINEASRIG 182

Query: 225 VPPNMSDAHTINGKSGPLFPCSEKHTFAMEVETGKTYLLRIINAALNDELFFAIAGHNLT 284
             P+ SDAHTING SG +  C  + ++ + V  GKTY+LRIINAALN+ELFF IAGH LT
Sbjct: 183 TAPSASDAHTINGHSGSISNCPSQSSYGLPVRAGKTYMLRIINAALNEELFFKIAGHVLT 242

Query: 285 VVEVDAVYTKSFSTQAILIAPGQTTNVLLQAN-QAPNRYFMASRSFMDVPIPVDNKTATG 344
           VVEVDAVYTK + T  + IAPGQTTNVLL AN  A + Y +A+ +F D  IP DN TAT 
Sbjct: 243 VVEVDAVYTKPYKTDTVFIAPGQTTNVLLTANANAGSNYMVAATTFTDAHIPYDNVTATA 302

Query: 345 ILQYKGIPNTI----LPILPQLPASNDTSFALNYNKKLKSLNSPQYPANVPLKVDRKLFY 404
            L Y G  +T+      +L  LP  N T  A  + + L+SLNS +YPA VP  V+  LF+
Sbjct: 303 TLHYIGHTSTVSTSKKTVLASLPPQNATWVATKFTRSLRSLNSLEYPARVPTTVEHSLFF 362

Query: 405 TIGLGQNACPTCLNGTQFVASLNNISFVMPQIGLLQSHYFNISGVFRTDFPDRPPTPFNY 464
           T+GLG N C +C NG + VA +NN++F MP+  LLQ+H+FNISGVF  DFP +P  P++Y
Sbjct: 363 TVGLGANPCQSCNNGVRLVAGINNVTFTMPKTALLQAHFFNISGVFTDDFPAKPSNPYDY 422

Query: 465 TG-APLTANLRTAVGTRLSKIAFNSTVELVLQDTNLLTVESHPFHLHGYNFFVVGTGIGN 524
           T    L  N  T  GT+L ++ +N+TV++VLQ+T ++  ++HPFHLHG+NFF VG G+GN
Sbjct: 423 TAPVKLGVNAATMKGTKLYRLPYNATVQIVLQNTAMILSDNHPFHLHGFNFFEVGRGLGN 482

Query: 525 FNPAKDPAKYNLVDPVERNTVGVPTGGWTAIRFRADNPGVWFMHCHLEVHTGWGLKTAFV 584
           FNP KDP  +NLVDPVERNTVGVP GGWTAIRF ADNPGVWFMHCHLE+HT WGLK AFV
Sbjct: 483 FNPEKDPKAFNLVDPVERNTVGVPAGGWTAIRFIADNPGVWFMHCHLELHTTWGLKMAFV 523

Query: 585 VEDGPGKDQTILPPPKDLPPC 600
           V++G G DQ++LPPP DLP C
Sbjct: 543 VDNGHGPDQSLLPPPADLPKC 523

BLAST of Lsi07G002590 vs. TAIR 10
Match: AT5G60020.1 (laccase 17 )

HSP 1 Score: 646.7 bits (1667), Expect = 1.8e-185
Identity = 318/606 (52.48%), Postives = 403/606 (66.50%), Query Frame = 0

Query: 16  LLFVACCLGLISFSAEAAIKRYQFDVQVQNISRLCHAKPIVTVNGKFPGPTIYVQEGDRV 75
           LL V  C+ L+   A    + Y  ++++QN++RLCH K +V+VNG+FPGP +  +EGD+V
Sbjct: 6   LLAVFSCVLLLPQPAFGITRHYTLEIKMQNVTRLCHTKSLVSVNGQFPGPKLIAREGDQV 65

Query: 76  LVNVTNHAQYNMTLMTRVKIEPPPTPQYMCVFIYVTWVLISVAFCIFCRHGLKQYRNGWA 135
           L+ V N    N++L                      W            HG++Q R+GWA
Sbjct: 66  LIKVVNQVPNNISL---------------------HW------------HGIRQLRSGWA 125

Query: 136 DGPAYITQCPIQRGNSYTYDFNVTGQRGTLWWHAHILWLRATVYGAIVIMPKQGTPYPFP 195
           DGPAYITQCPIQ G SY Y++ + GQRGTLW+HAHI WLR+TVYG ++I+PK+G PYPF 
Sbjct: 126 DGPAYITQCPIQTGQSYVYNYTIVGQRGTLWYHAHISWLRSTVYGPLIILPKRGVPYPFA 185

Query: 196 QPNREFEILLGEWWNNDVEAVVNQGSSMGVPPNMSDAHTINGKSGPLFPCSEKHTFAMEV 255
           +P++E  ++ GEW+N D EA++ Q +  G  PN+SDA+TING  GPL+ CS K TF + V
Sbjct: 186 KPHKEVPMIFGEWFNADTEAIIRQATQTGGGPNVSDAYTINGLPGPLYNCSAKDTFRLRV 245

Query: 256 ETGKTYLLRIINAALNDELFFAIAGHNLTVVEVDAVYTKSFSTQAILIAPGQTTNVLLQA 315
           + GKTYLLR+INAALNDELFF+IA H +TVVE DA+Y K F T+ ILIAPGQTTNVLL+ 
Sbjct: 246 KPGKTYLLRLINAALNDELFFSIANHTVTVVEADAIYVKPFETETILIAPGQTTNVLLKT 305

Query: 316 NQA--PNRYFMASRSFMDVPIPVDNKTATGILQYKGIPNT-----------ILPILPQLP 375
             +     +FM +R ++      DN T  GIL+Y+    T           +    P LP
Sbjct: 306 KSSYPSASFFMTARPYVTGQGTFDNSTVAGILEYEPPKQTKGAHSRTSIKNLQLFKPILP 365

Query: 376 ASNDTSFALNYNKKLKSLNSPQYPANVPLKVDRKLFYTIGLGQNAC-----PTC---LNG 435
           A NDT+FA  ++ KL+SLNS  +PANVPL VDRK F+T+GLG N C      TC    N 
Sbjct: 366 ALNDTNFATKFSNKLRSLNSKNFPANVPLNVDRKFFFTVGLGTNPCNHKNNQTCQGPTNT 425

Query: 436 TQFVASLNNISFVMPQIGLLQSHYFNIS-GVFRTDFPDRPPTPFNYTGAPLTANLRTAVG 495
           T F AS++NISF MP   LLQSHY   S GV+   FP  P  PFNYTG P   N   + G
Sbjct: 426 TMFAASISNISFTMPTKALLQSHYSGQSHGVYSPKFPWSPIVPFNYTGTP-PNNTMVSNG 485

Query: 496 TRLSKIAFNSTVELVLQDTNLLTVESHPFHLHGYNFFVVGTGIGNFNPAKDPAKYNLVDP 555
           T L  + +N++VELV+QDT++L  ESHP HLHG+NFFVVG G GNF+P KDP  +NLVDP
Sbjct: 486 TNLMVLPYNTSVELVMQDTSILGAESHPLHLHGFNFFVVGQGFGNFDPNKDPRNFNLVDP 545

Query: 556 VERNTVGVPTGGWTAIRFRADNPGVWFMHCHLEVHTGWGLKTAFVVEDGPGKDQTILPPP 600
           +ERNTVGVP+GGW AIRF ADNPGVWFMHCHLEVHT WGL+ A++V DG   DQ +LPPP
Sbjct: 546 IERNTVGVPSGGWAAIRFLADNPGVWFMHCHLEVHTSWGLRMAWLVLDGDKPDQKLLPPP 577

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8VZA13.5e-26371.65Laccase-11 OS=Arabidopsis thaliana OX=3702 GN=LAC11 PE=2 SV=1[more]
Q8RYM91.4e-24066.16Laccase-2 OS=Oryza sativa subsp. japonica OX=39947 GN=LAC2 PE=2 SV=1[more]
O804347.0e-20355.59Laccase-4 OS=Arabidopsis thaliana OX=3702 GN=IRX12 PE=2 SV=2[more]
Q0IQU11.7e-20155.59Laccase-22 OS=Oryza sativa subsp. japonica OX=39947 GN=LAC22 PE=2 SV=2[more]
Q1PDH61.6e-19454.55Laccase-16 OS=Arabidopsis thaliana OX=3702 GN=LAC16 PE=2 SV=2[more]
Match NameE-valueIdentityDescription
A0A5D3D2C10.0e+0090.60Laccase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold21G004900 PE=3 S... [more]
A0A1S3BU500.0e+0090.60Laccase OS=Cucumis melo OX=3656 GN=LOC103493188 PE=3 SV=1[more]
A0A0A0KWQ30.0e+0090.10Laccase OS=Cucumis sativus OX=3659 GN=Csa_4G046770 PE=3 SV=1[more]
A0A6J1EN140.0e+0088.96Laccase OS=Cucurbita moschata OX=3662 GN=LOC111435985 PE=3 SV=1[more]
A0A6J1JB060.0e+0089.26Laccase OS=Cucurbita maxima OX=3661 GN=LOC111482840 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_038905726.10.0e+0093.62laccase-11-like [Benincasa hispida][more]
XP_008452071.10.0e+0090.60PREDICTED: laccase-11-like [Cucumis melo] >TYK16659.1 laccase-11-like [Cucumis m... [more]
XP_023552611.10.0e+0089.46laccase-11-like [Cucurbita pepo subsp. pepo][more]
XP_004146556.10.0e+0090.10laccase-11 [Cucumis sativus] >KGN53314.1 hypothetical protein Csa_015210 [Cucumi... [more]
XP_022929401.10.0e+0088.96laccase-11-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
AT5G03260.12.5e-26471.65laccase 11 [more]
AT2G38080.15.0e-20455.59Laccase/Diphenol oxidase family protein [more]
AT5G01190.14.8e-19154.45laccase 10 [more]
AT5G58910.15.4e-19055.44laccase 16 [more]
AT5G60020.11.8e-18552.48laccase 17 [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001117Multicopper oxidase, type 1PFAMPF00394Cu-oxidasecoord: 203..349
e-value: 5.2E-41
score: 140.4
IPR008972CupredoxinGENE3D2.60.40.420coord: 26..186
e-value: 8.5E-31
score: 108.9
IPR008972CupredoxinGENE3D2.60.40.420coord: 407..599
e-value: 4.7E-59
score: 201.6
IPR008972CupredoxinGENE3D2.60.40.420coord: 198..382
e-value: 7.6E-41
score: 142.4
IPR008972CupredoxinSUPERFAMILY49503Cupredoxinscoord: 194..374
IPR008972CupredoxinSUPERFAMILY49503Cupredoxinscoord: 29..211
IPR008972CupredoxinSUPERFAMILY49503Cupredoxinscoord: 390..599
IPR011706Multicopper oxidase, C-terminalPFAMPF07731Cu-oxidase_2coord: 449..581
e-value: 3.8E-40
score: 136.8
IPR011707Multicopper oxidase, N-termianlPFAMPF07732Cu-oxidase_3coord: 125..188
e-value: 1.1E-20
score: 73.8
coord: 41..89
e-value: 3.0E-11
score: 43.4
IPR017761LaccaseTIGRFAMTIGR03389TIGR03389coord: 124..599
e-value: 8.5E-250
score: 828.0
NoneNo IPR availablePANTHERPTHR11709:SF116LACCASEcoord: 124..599
NoneNo IPR availablePANTHERPTHR11709:SF116LACCASEcoord: 15..89
IPR045087Multicopper oxidasePANTHERPTHR11709MULTI-COPPER OXIDASEcoord: 124..599
IPR045087Multicopper oxidasePANTHERPTHR11709MULTI-COPPER OXIDASEcoord: 15..89
IPR033138Multicopper oxidases, conserved sitePROSITEPS00079MULTICOPPER_OXIDASE1coord: 557..577
IPR002355Multicopper oxidase, copper-binding sitePROSITEPS00080MULTICOPPER_OXIDASE2coord: 562..573
IPR034285Laccase, second cupredoxin domainCDDcd13875CuRO_2_LCC_plantcoord: 203..347
e-value: 3.19174E-84
score: 257.912
IPR034288Laccase, first cupredoxin domainCDDcd13849CuRO_1_LCC_plantcoord: 37..186
e-value: 1.27366E-62
score: 200.178
IPR034289Laccase, third cupredoxin domainCDDcd13897CuRO_3_LCC_plantcoord: 444..582
e-value: 9.55584E-86
score: 261.425

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi07G002590.1Lsi07G002590.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0046274 lignin catabolic process
cellular_component GO:0048046 apoplast
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0005507 copper ion binding
molecular_function GO:0052716 hydroquinone:oxygen oxidoreductase activity
molecular_function GO:0016491 oxidoreductase activity