Lsi06G014860 (gene) Bottle gourd (USVL1VR-Ls) v1

BLAST of Lsi06G014860 vs. ExPASy Swiss-Prot
Match: Q8H157 (Protein NRT1/ PTR FAMILY 4.6 OS=Arabidopsis thaliana OX=3702 GN=NPF4.6 PE=1 SV=1)

HSP 1 Score: 709.1 bits (1829), Expect = 4.3e-203
Identity = 371/587 (63.20%), Postives = 450/587 (76.66%), Query Frame = 0

Query: 15  VEETQQLNLWEDYVDWRKRPAVKGRHGGMLAASFVLVVEVLENLAFLANASNLVLYLSKF 74
           +E  ++++ WE Y DWR R AVKGRHGGMLAASFVLVVE+LENLA+LANASNLVLYL ++
Sbjct: 1   MEVEEEVSRWEGYADWRNRAAVKGRHGGMLAASFVLVVEILENLAYLANASNLVLYLREY 60

Query: 75  MHYSPSESANIVTNFMGTAFLLALLGGFLADAFFTTYSIFLISAAIESLGQAGCPV--RL 134
           MH SPS+SAN VTNFMGTAFLLALLGGFL+DAFF+T+ IFLISA+IE LG     +  R 
Sbjct: 61  MHMSPSKSANDVTNFMGTAFLLALLGGFLSDAFFSTFQIFLISASIEFLGLIILTIQART 120

Query: 135 VKVRPARCVTNAAGNGNPCHKVGGGEAVMLFAGLYMVALGVGGIKGSLPPHGAEQFDETS 194
             + P  C      +   C +V G +A MLF GLY+VALGVGGIKGSL  HGAEQFDE++
Sbjct: 121 PSLMPPSC------DSPTCEEVSGSKAAMLFVGLYLVALGVGGIKGSLASHGAEQFDEST 180

Query: 195 FEGRKKRSAFFNYFIFCLSCGALIAVTLVVWMEDNKGWQWGFGISTLTILISIPIFLLGS 254
            +GRK+RS FFNYF+FCL+CGAL+AVT VVW+EDNKGW+WGFG+ST+ I +SI IFL GS
Sbjct: 181 PKGRKQRSTFFNYFVFCLACGALVAVTFVVWLEDNKGWEWGFGVSTIAIFVSILIFLSGS 240

Query: 255 PTYRIKTPAGSPITTIFKVLASAAFNSRKAKTCNNVVMNISTSSVSSDTTTDIILDEQRQ 314
             YR K P GSP+TTI KVL +A+     + + +N V ++S S  +   +      E + 
Sbjct: 241 RFYRNKIPCGSPLTTILKVLLAASVKCCSSGSSSNAVASMSVSPSNHCVSKGKKEVESQG 300

Query: 315 NMEKENNPITIPIPVS-TQAMEFLNKATINNPNHPQLNCTVKQVEEAKIVLKIFPIFTST 374
            +EK      +P     T +++ LN A    P H  L CTV+QVE+ KIVLK+ PIF  T
Sbjct: 301 ELEKPRQEEALPPRAQLTNSLKVLNGAADEKPVHRLLECTVQQVEDVKIVLKMLPIFACT 360

Query: 375 IMLNCCLAQLSTFSVQQAATMNTKLGSFKVPPASLPVFPVIFIMILAPTYNHFILPIARK 434
           IMLNCCLAQLSTFSVQQAA+MNTK+GS K+PPASLP+FPV+FIMILAP Y+H I+P ARK
Sbjct: 361 IMLNCCLAQLSTFSVQQAASMNTKIGSLKIPPASLPIFPVVFIMILAPIYDHLIIPFARK 420

Query: 435 LTKSEMGITHLQRIGTGLVLSTVAMAVAALIETKRKTLAMKIHKLDSSEPLPITFLWVAL 494
            TK+E G+THLQRIG GLVLS +AMAVAAL+E KRK +A     LDS E LP+TFLW+AL
Sbjct: 421 ATKTETGVTHLQRIGVGLVLSILAMAVAALVEIKRKGVAKDSGLLDSKETLPVTFLWIAL 480

Query: 495 QYLFLGSADLFSLAGMMEFFFTEAPLSMRSLATALSWASLAMGYFLSSVLVTLVNGVTKA 554
           QYLFLGSADLF+LAG++E+FFTEAP SMRSLAT+LSWASLAMGY+LSSV+V++VN +T +
Sbjct: 481 QYLFLGSADLFTLAGLLEYFFTEAPSSMRSLATSLSWASLAMGYYLSSVIVSIVNSITGS 540

Query: 555 CGFRPWLYGKSLNRYHLERFYWVMCVLSGLNFFQYLFWANRYTYRST 599
            G  PWL GKS+NRY L+ FYW+MCVLS  NF  YLFWA RY YRST
Sbjct: 541 SGNTPWLRGKSINRYKLDYFYWLMCVLSAANFLHYLFWAMRYKYRST 581

BLAST of Lsi06G014860 vs. ExPASy Swiss-Prot
Match: Q9FM20 (Protein NRT1/ PTR FAMILY 4.7 OS=Arabidopsis thaliana OX=3702 GN=NPF4.7 PE=2 SV=2)

HSP 1 Score: 694.5 bits (1791), Expect = 1.1e-198
Identity = 374/592 (63.18%), Postives = 444/592 (75.00%), Query Frame = 0

Query: 15  VEETQQLNLWEDYVDWRKRPAVKGRHGGMLAASFVLVVEVLENLAFLANASNLVLYLSKF 74
           ++E  +L+ W  YVDWR RPA++GRHGGMLAASFVLVVEVLENLAFLANASNLVLYLS  
Sbjct: 32  MDEANRLSAWNGYVDWRSRPALRGRHGGMLAASFVLVVEVLENLAFLANASNLVLYLSTK 91

Query: 75  MHYSPSESANIVTNFMGTAFLLALLGGFLADAFFTTYSIFLISAAIESLGQAGCPVRLVK 134
           M +SPS +AN VT FMGTAF LALLGGFLADAFFTT+ I+L+SAAIE LG     V+  +
Sbjct: 92  MGFSPSGAANAVTAFMGTAFFLALLGGFLADAFFTTFHIYLVSAAIEFLGLMVLTVQAHE 151

Query: 135 VRPARCVTNAAGNGNPCHKVGGGEAVMLFAGLYMVALGVGGIKGSLPPHGAEQFDETSFE 194
                            H       V LF GLY+VALGVGGIKGSLPPHGAEQFDE +  
Sbjct: 152 -----------------HSTEPWSRVFLFVGLYLVALGVGGIKGSLPPHGAEQFDEETSS 211

Query: 195 GRKKRSAFFNYFIFCLSCGALIAVTLVVWMEDNKGWQWGFGISTLTILISIPIFLLGSPT 254
           GR++RS FFNYFIF LSCGALIAVT+VVW+EDNKGW +GFG+ST  ILIS+P+FL GS  
Sbjct: 212 GRRQRSFFFNYFIFSLSCGALIAVTVVVWLEDNKGWSYGFGVSTAAILISVPVFLAGSRV 271

Query: 255 YRIKTPAGSPITTIFKVLASAAFNSRKAKTCNNVVMNISTSSVSSDTTTDIILDEQRQNM 314
           YR+K P+GSPITT+FKVL +A +   K +  + +V+   T +   D+ T       +QN 
Sbjct: 272 YRLKVPSGSPITTLFKVLTAALYAKYKKRRTSRIVVTCHTRNDCDDSVT-------KQNC 331

Query: 315 EKENNPITIPIPVSTQAMEFLNKATINNPNHPQ-LNCTVKQVEEAKIVLKIFPIFTSTIM 374
           + ++  +            FL +      + P+ L CT +QV++ KIV+KI PIF STIM
Sbjct: 332 DGDDGFLG----------SFLGEVVRERESLPRPLRCTEEQVKDVKIVIKILPIFMSTIM 391

Query: 375 LNCCLAQLSTFSVQQAATMNTKLGSFKVPPASLPVFPVIFIMILAPTYNHFILPIARKLT 434
           LNCCLAQLSTFSVQQA+TMNTKLGSF VPPA+LPVFPV+F+MILAPTYNH +LP+ARK T
Sbjct: 392 LNCCLAQLSTFSVQQASTMNTKLGSFTVPPAALPVFPVVFMMILAPTYNHLLLPLARKST 451

Query: 435 KSEMGITHLQRIGTGLVLSTVAMAVAALIETKRKTLAMKIHKLD-----SSEPLPITFLW 494
           K+E GITHLQRIGTGLVLS VAMAVAAL+ETKRK + +     +     SS PLPITFLW
Sbjct: 452 KTETGITHLQRIGTGLVLSIVAMAVAALVETKRKHVVVSCCSNNNSSSYSSSPLPITFLW 511

Query: 495 VALQYLFLGSADLFSLAGMMEFFFTEAPLSMRSLATALSWASLAMGYFLSSVLVTLVNGV 554
           VA+QY+FLGSADLF+LAGMMEFFFTEAP +MRSLAT+LSWASLAMGY+ SSVLV+ VN V
Sbjct: 512 VAIQYVFLGSADLFTLAGMMEFFFTEAPSTMRSLATSLSWASLAMGYYFSSVLVSAVNFV 571

Query: 555 TKACGFRPWLYGKSLNRYHLERFYWVMCVLSGLNFFQYLFWANRYTYRSTNG 601
           T      PWL G++LN+YHLERFYW+MCVLSG+NF  YLFWA+RY YRS  G
Sbjct: 572 TGLNHHNPWLLGENLNQYHLERFYWLMCVLSGINFLHYLFWASRYVYRSNQG 589

BLAST of Lsi06G014860 vs. ExPASy Swiss-Prot
Match: Q8VYE4 (Protein NRT1/ PTR FAMILY 4.5 OS=Arabidopsis thaliana OX=3702 GN=NPF4.5 PE=2 SV=1)

HSP 1 Score: 677.2 bits (1746), Expect = 1.8e-193
Identity = 364/586 (62.12%), Postives = 438/586 (74.74%), Query Frame = 0

Query: 15  VEETQQLNLWEDYVDWRKRPAVKGRHGGMLAASFVLVVEVLENLAFLANASNLVLYLSKF 74
           VE    ++ WE Y DWR + A++GRHGGMLAASFVL VE+LENLAFLANASNLVLYL  F
Sbjct: 3   VEMHGDVSKWEGYADWRNKAALRGRHGGMLAASFVLAVEILENLAFLANASNLVLYLKNF 62

Query: 75  MHYSPSESANIVTNFMGTAFLLALLGGFLADAFFTTYSIFLISAAIESLGQAGCPV--RL 134
           MH S + S++ VT FM TAFLLALLGGFLADAFF+T+ IFLISA+IE LG     +  R 
Sbjct: 63  MHMSLARSSSEVTTFMATAFLLALLGGFLADAFFSTFVIFLISASIEFLGLILLTIQARR 122

Query: 135 VKVRPARCVTNAAGNGNPCHKVGGGEAVMLFAGLYMVALGVGGIKGSLPPHGAEQFDETS 194
             + P  C ++AA     C  VGG +A  LF GLY+V+LG+GGIKGSLP HGAEQFDE +
Sbjct: 123 PSLMPPPCKSSAALR---CEVVGGSKAAFLFVGLYLVSLGIGGIKGSLPSHGAEQFDEGT 182

Query: 195 FEGRKKRSAFFNYFIFCLSCGALIAVTLVVWMEDNKGWQWGFGISTLTILISIPIFLLGS 254
            +GRK+RS FFNY++FCLSCGAL+AVT VVW+EDNKGW+WGFG+ST++I +SI +FLLGS
Sbjct: 183 PKGRKQRSTFFNYYVFCLSCGALVAVTFVVWIEDNKGWEWGFGVSTISIFLSILVFLLGS 242

Query: 255 PTYRIKTPAGSPITTIFKVLASAAFNSRKAKTCNNVVMNISTSSVSSDTTTDIILDEQRQ 314
             Y+ K P GSP+TTIFKVL +A+  S  +KT +N   +    S   + T          
Sbjct: 243 RFYKNKIPRGSPLTTIFKVLLAASIVSCSSKTSSNHFTSREVQSEHEEKT---------- 302

Query: 315 NMEKENNPITIPIPVSTQAMEFLNKATINNPNHPQLNCTVKQVEEAKIVLKIFPIFTSTI 374
                      P    T ++  LNKA     +H  L CTV+QVE+ KIVLK+ PIF  TI
Sbjct: 303 -----------PSQSLTNSLTCLNKAIEGKTHHIWLECTVQQVEDVKIVLKMLPIFGCTI 362

Query: 375 MLNCCLAQLSTFSVQQAATMNTKLGSFKVPPASLPVFPVIFIMILAPTYNHFILPIARKL 434
           MLNCCLAQLST+SV QAATMN K+ +F VP ASLPVFPV+F++ILAPTY+H I+P ARK+
Sbjct: 363 MLNCCLAQLSTYSVHQAATMNRKIVNFNVPSASLPVFPVVFMLILAPTYDHLIIPFARKV 422

Query: 435 TKSEMGITHLQRIGTGLVLSTVAMAVAALIETKRKTLAMKIHKLDSSEPLPITFLWVALQ 494
           TKSE+GITHLQRIG GLVLS VAMAVAAL+E KRK +A +   LDS E LPITFLW+ALQ
Sbjct: 423 TKSEIGITHLQRIGVGLVLSIVAMAVAALVELKRKQVAREAGLLDSEETLPITFLWIALQ 482

Query: 495 YLFLGSADLFSLAGMMEFFFTEAPLSMRSLATALSWASLAMGYFLSSVLVTLVNGVTKAC 554
           YLFLGSADLF+LAG++EFFFTEAP SMRSLAT+LSWASLA+GY+LSSV+V +VN VTK+ 
Sbjct: 483 YLFLGSADLFTLAGLLEFFFTEAPSSMRSLATSLSWASLALGYYLSSVMVPIVNRVTKSA 542

Query: 555 GFRPWLYGKSLNRYHLERFYWVMCVLSGLNFFQYLFWANRYTYRST 599
           G  PWL G+ LNR  L+ FYW+MCVLS +NF  YLFWA RY Y ST
Sbjct: 543 GQSPWL-GEKLNRNRLDLFYWLMCVLSVVNFLHYLFWAKRYKYIST 563

BLAST of Lsi06G014860 vs. ExPASy Swiss-Prot
Match: Q9LSF0 (Protein NRT1/ PTR FAMILY 4.1 OS=Arabidopsis thaliana OX=3702 GN=NPF4.1 PE=2 SV=1)

HSP 1 Score: 382.9 bits (982), Expect = 7.0e-105
Identity = 234/576 (40.62%), Postives = 334/576 (57.99%), Query Frame = 0

Query: 20  QLNLWEDYVDWRKRPAVKGRHGGMLAASFVLVVEVLENLAFLANASNLVLYLSKFMHYSP 79
           Q+ + E + DWR + A+ G+HGG+ AA    VVE +EN+ FLA ++N ++Y +K M+YS 
Sbjct: 2   QIEMEEKFEDWRGKEAISGKHGGIKAAFIACVVETMENMVFLACSTNFMMYFTKSMNYST 61

Query: 80  SESANIVTNFMGTAFLLALLGGFLADAFFTTYSIFLISAAIESLGQAGCPVRLVKVRPAR 139
            ++A +VTNF+GT+FLL + GGF+AD+F T ++ F++  +IE LG       L+ +    
Sbjct: 62  PKAATMVTNFVGTSFLLTIFGGFVADSFLTRFAAFVLFGSIELLG-------LIMLTLQA 121

Query: 140 CVTNAAGNGNPCHKVGGGEAVMLFAGLYMVALGVGGIKGSLPPHGAEQFDETSFEGRKKR 199
            +T     G    K    ++ +LF GLY +A+GVGG+KGSLP HG    D+     ++  
Sbjct: 122 HITKLQPQGG--KKPSTPQSTVLFTGLYAIAIGVGGVKGSLPAHGG---DQIGTRNQRLI 181

Query: 200 SAFFNYFIFCLSCGALIAVTLVVWMEDNKGWQWGFGISTLTILISIPIFLLGSPTYRIKT 259
           S FFN++ F +  G  +AVTL+VW+E+N GW   F IST  +  +I +F+ G P YR K 
Sbjct: 182 SGFFNWYFFSVCLGGFLAVTLMVWIEENIGWSSSFTISTAVLASAIFVFVAGCPMYRFKR 241

Query: 260 PAGSPITTIFKVLASAAFNSRKAKTCNNVVMNISTSSVSSDTTTDIILDEQRQNMEKENN 319
           PAGSP+T I  V  SAA N  +  T   VV     S+  S                    
Sbjct: 242 PAGSPLTRIVNVFVSAARNRNRFVTDAEVVTQNHNSTDKS-------------------- 301

Query: 320 PITIPIPVSTQAMEFLNKATINNPNHPQLNCTVKQVEEAKIVLKIFPIFTSTIMLNCCLA 379
                  +     +FLNKA +NN        +  QVEE +  L + PIF STI++NCC+A
Sbjct: 302 -------IHHNKFKFLNKAKLNN------KISATQVEETRTFLALLPIFGSTIIMNCCVA 361

Query: 380 QLSTFSVQQAATMNTKLG-SFKVPPASLPVFPVIFIMILAPTYNHFILPIARKLTKSEMG 439
           Q+ TFSVQQ    N KL  SF++P ASL   P++ ++     Y  F     R L+ SE  
Sbjct: 362 QMGTFSVQQGMVTNRKLSRSFEIPVASLNAIPLLCMLSSLALYELF---GKRILSNSERS 421

Query: 440 IT-HLQRIGTGLVLSTVAMAVAALIETKRKTLAMKIHKLDSSEPLPITFLWVALQYLFLG 499
            + +L+RIG GL L++++MAVAA++E KRK  A+          + I+  W+ LQ++ L 
Sbjct: 422 SSFNLKRIGYGLALTSISMAVAAIVEVKRKHEAV-------HNNIKISVFWLELQFVMLS 481

Query: 500 SADLFSLAGMMEFFFTEAPLSMRSLATALSWASLAMGYFLSSVLVTLVNGVTKACGFRPW 559
            +D+ ++ GM+EFFF E+P SMRS++TAL W S AMG+FLSSVLV +VNG+T       W
Sbjct: 482 LSDMLTVGGMLEFFFRESPASMRSMSTALGWCSTAMGFFLSSVLVEVVNGIT------GW 515

Query: 560 LYGKSLNRYHLERFYWVMCVLSGLNFFQYLFWANRY 594
           L    LN   LE FY V+CVL+ LN F Y+FWA RY
Sbjct: 542 L-RDDLNESRLELFYLVLCVLNTLNLFNYIFWAKRY 515

BLAST of Lsi06G014860 vs. ExPASy Swiss-Prot
Match: Q9LSE8 (Protein NRT1/ PTR FAMILY 4.2 OS=Arabidopsis thaliana OX=3702 GN=NPF4.2 PE=2 SV=1)

HSP 1 Score: 377.5 bits (968), Expect = 2.9e-103
Identity = 232/576 (40.28%), Postives = 335/576 (58.16%), Query Frame = 0

Query: 25  EDYVDWRKRPAVKGRHGGMLAASFVLVVEVLENLAFLANASNLVLYLSKFMHYSPSESAN 84
           E + DW+ + A+ G+HGG+ AAS V VV ++EN+ F+AN  N V Y    MHY+P+ +AN
Sbjct: 7   EKFEDWKGKEAIPGKHGGIRAASIVCVVVMMENIVFIANGFNFVKYFMGSMHYTPATAAN 66

Query: 85  IVTNFMGTAFLLALLGGFLADAFFTTYSIFLISAAIESLG------QAGCPVRLVKVRPA 144
           +VTNFMGT+FLL L GGF+AD+F T ++ F++   IE +G      QA  P    K+ P 
Sbjct: 67  MVTNFMGTSFLLTLFGGFIADSFVTHFTTFIVFCCIELMGLILLTFQAHNP----KLLPE 126

Query: 145 RCVTNAAGNGNPCHKVGGGEAVMLFAGLYMVALGVGGIKGSLPPHGAEQFDETSFEGRKK 204
           +  T +             ++ +LF GLY +A+G GG+K SLP HG +Q D       + 
Sbjct: 127 KDKTPST-----------LQSAILFTGLYAMAIGTGGLKASLPSHGGDQIDR---RNPRL 186

Query: 205 RSAFFNYFIFCLSCGALIAVTLVVWMEDNKGWQWGFGISTLTILISIPIFLLGSPTYRIK 264
            S FF++  F +  G L+AVT+V+W+E+ KGW W F IS   +  ++ IF +G P YR K
Sbjct: 187 ISRFFDWLYFSICSGCLLAVTVVLWIEEKKGWIWSFNISVGILATALCIFTVGLPFYRFK 246

Query: 265 TPAGSPITTIFKVLASAAFNSRKAKTCNNVVMNISTSSVSSDTTTDIILDEQRQNMEKEN 324
            P GSP+  I  V+ SAA N  K+                        LDE     E   
Sbjct: 247 RPNGSPLKKIAIVIISAARNRNKSD-----------------------LDE-----EMMR 306

Query: 325 NPITIPIPVSTQAMEFLNKATINNPNHPQLNCTVKQVEEAKIVLKIFPIFTSTIMLNCCL 384
             I+I    S   +++++KAT+N       N +  +VEE +  L + PIF STI+++CC+
Sbjct: 307 GLISIYKNNSHNKLKWIDKATLNK------NISETEVEETRTFLGLLPIFGSTIVMSCCV 366

Query: 385 AQLSTFSVQQAATMNTKL-GSFKVPPASLPVFPVIFIMILAPTYNHFILPIARKLTKSEM 444
           AQLSTFS QQ   MN KL  SF++P  SL   P+IF+++  P Y  F   I+     +  
Sbjct: 367 AQLSTFSAQQGMLMNKKLFHSFEIPVPSLTAIPLIFMLLSIPLYEFFGKKISSG-NNNRS 426

Query: 445 GITHLQRIGTGLVLSTVAMAVAALIETKRKTLAMKIHKLDSSEPLPITFLWVALQYLFLG 504
              +L+RIG GL LS+V+MAV+A++E KRK      H++  +    I+ LW+  QYL L 
Sbjct: 427 SSFNLKRIGLGLALSSVSMAVSAIVEAKRK------HEVVHNN-FRISVLWLVFQYLMLS 486

Query: 505 SADLFSLAGMMEFFFTEAPLSMRSLATALSWASLAMGYFLSSVLVTLVNGVTKACGFRPW 564
            +D+ +L GM+EFF+ EAP +M+S++TAL W S A+G+FLS+ LV + N VT   G + W
Sbjct: 487 VSDMLTLGGMLEFFYREAPSNMKSISTALGWCSTALGFFLSTTLVEVTNAVTGRLGHQ-W 521

Query: 565 LYGKSLNRYHLERFYWVMCVLSGLNFFQYLFWANRY 594
           L G+ LN+  LE FY ++CVL+ LN   Y+FWA RY
Sbjct: 547 LGGEDLNKTRLELFYVLLCVLNTLNLLNYIFWAKRY 521

BLAST of Lsi06G014860 vs. ExPASy TrEMBL
Match: A0A5A7UWF3 (Protein NRT1/ PTR FAMILY 4.6-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold274G002280 PE=3 SV=1)

HSP 1 Score: 1076.2 bits (2782), Expect = 0.0e+00
Identity = 556/626 (88.82%), Postives = 582/626 (92.97%), Query Frame = 0

Query: 1   MSSITVTPLETQNHVEETQQLNLWEDYVDWRKRPAVKGRHGGMLAASFVLVVEVLENLAF 60
           MSSIT TPL+T+ HVEETQ+LNL EDYVDWRKRPAVKGRHGGMLAASFVLVVEVLENLAF
Sbjct: 1   MSSITATPLQTEKHVEETQRLNLCEDYVDWRKRPAVKGRHGGMLAASFVLVVEVLENLAF 60

Query: 61  LANASNLVLYLSKFMHYSPSESANIVTNFMGTAFLLALLGGFLADAFFTTYSIFLISAAI 120
           LANASNLVLYLSKFMHYSPSESANIVTNFMGTAFLLALLGGFLADAFFTTYSIFLISAAI
Sbjct: 61  LANASNLVLYLSKFMHYSPSESANIVTNFMGTAFLLALLGGFLADAFFTTYSIFLISAAI 120

Query: 121 ESLG------QAGCPVRLVKVRPARCVTNAAGNGNPCHKVGGGEAVMLFAGLYMVALGVG 180
           ESLG      QA  P     ++PARCV+NA GNGNPCHKV GGEA MLFAGLYMVALGVG
Sbjct: 121 ESLGLVILTLQADVP----HLKPARCVSNAVGNGNPCHKVEGGEAAMLFAGLYMVALGVG 180

Query: 181 GIKGSLPPHGAEQFDETSFEGRKKRSAFFNYFIFCLSCGALIAVTLVVWMEDNKGWQWGF 240
           GIKG+LPPHGAEQFDETSFEGRKKRS FFNYFIFCLSCGALIAVTLVVWMEDNKGWQWGF
Sbjct: 181 GIKGALPPHGAEQFDETSFEGRKKRSVFFNYFIFCLSCGALIAVTLVVWMEDNKGWQWGF 240

Query: 241 GISTLTILISIPIFLLGSPTYRIKTPAGSPITTIFKVLASAAFNSRKAKTCNNVVMNIST 300
           GISTLTILISIPIFLLGSPTYRIKTPAGSPITTIFKVL SAAFNSRK KT NNVV+NIST
Sbjct: 241 GISTLTILISIPIFLLGSPTYRIKTPAGSPITTIFKVLVSAAFNSRKKKTRNNVVINIST 300

Query: 301 SSVSSDTTTDIILDEQRQNMEKENNPITIPIPVSTQAMEFLNKATINNPNHPQLNCTVKQ 360
           SSVSSD TTDII+DEQRQ +EKEN+    PIP+ TQAMEFLNKATI+NPNHPQLNCTVKQ
Sbjct: 301 SSVSSDATTDIIVDEQRQTLEKEND----PIPIQTQAMEFLNKATIDNPNHPQLNCTVKQ 360

Query: 361 VEEAKIVLKIFPIFTSTIMLNCCLAQLSTFSVQQAATMNTKLGSFKVPPASLPVFPVIFI 420
           VEEAKIVLK+ PIFTSTIMLNCCLAQLSTFSVQQAATMNTKLGS KVPPASLPVFPV+FI
Sbjct: 361 VEEAKIVLKVLPIFTSTIMLNCCLAQLSTFSVQQAATMNTKLGSLKVPPASLPVFPVLFI 420

Query: 421 MILAPTYNHFILPIARKLTKSEMGITHLQRIGTGLVLSTVAMAVAALIETKRKTLAMKIH 480
           MILAPTYNH +LPIARKLTKSEMGITHLQRIGTGLVLSTVAMAVAAL+ETKRK LA+K H
Sbjct: 421 MILAPTYNHLVLPIARKLTKSEMGITHLQRIGTGLVLSTVAMAVAALVETKRKNLAIKTH 480

Query: 481 KLDSSEPLPITFLWVALQYLFLGSADLFSLAGMMEFFFTEAPLSMRSLATALSWASLAMG 540
           KLDS +PLPITFLWVALQYLFLGSADLFSLAGMMEFFFTEAPLSMRSLATALSWASLAMG
Sbjct: 481 KLDSPDPLPITFLWVALQYLFLGSADLFSLAGMMEFFFTEAPLSMRSLATALSWASLAMG 540

Query: 541 YFLSSVLVTLVNGVTKACGFRPWLYGKSLNRYHLERFYWVMCVLSGLNFFQYLFWANRYT 600
           Y+ SSVLVTLVNGVTKACG RPWLYGKSLN YHLERFYWVMC+LSGLNF QYLFWA+RYT
Sbjct: 541 YYFSSVLVTLVNGVTKACGLRPWLYGKSLNHYHLERFYWVMCILSGLNFLQYLFWASRYT 600

Query: 601 YRSTNGGEQSLKEQPNNAALVDERDV 621
           YRS NGGEQSLKEQP  + L++ER+V
Sbjct: 601 YRSINGGEQSLKEQPYKSDLIEEREV 618

BLAST of Lsi06G014860 vs. ExPASy TrEMBL
Match: A0A1S3BVK2 (protein NRT1/ PTR FAMILY 4.6-like OS=Cucumis melo OX=3656 GN=LOC103493972 PE=3 SV=1)

HSP 1 Score: 1076.2 bits (2782), Expect = 0.0e+00
Identity = 556/626 (88.82%), Postives = 582/626 (92.97%), Query Frame = 0

Query: 1   MSSITVTPLETQNHVEETQQLNLWEDYVDWRKRPAVKGRHGGMLAASFVLVVEVLENLAF 60
           MSSIT TPL+T+ HVEETQ+LNL EDYVDWRKRPAVKGRHGGMLAASFVLVVEVLENLAF
Sbjct: 1   MSSITATPLQTEKHVEETQRLNLCEDYVDWRKRPAVKGRHGGMLAASFVLVVEVLENLAF 60

Query: 61  LANASNLVLYLSKFMHYSPSESANIVTNFMGTAFLLALLGGFLADAFFTTYSIFLISAAI 120
           LANASNLVLYLSKFMHYSPSESANIVTNFMGTAFLLALLGGFLADAFFTTYSIFLISAAI
Sbjct: 61  LANASNLVLYLSKFMHYSPSESANIVTNFMGTAFLLALLGGFLADAFFTTYSIFLISAAI 120

Query: 121 ESLG------QAGCPVRLVKVRPARCVTNAAGNGNPCHKVGGGEAVMLFAGLYMVALGVG 180
           ESLG      QA  P     ++PARCV+NA GNGNPCHKV GGEA MLFAGLYMVALGVG
Sbjct: 121 ESLGLVILTLQADVP----HLKPARCVSNAVGNGNPCHKVEGGEAAMLFAGLYMVALGVG 180

Query: 181 GIKGSLPPHGAEQFDETSFEGRKKRSAFFNYFIFCLSCGALIAVTLVVWMEDNKGWQWGF 240
           GIKG+LPPHGAEQFDETSFEGRKKRS FFNYFIFCLSCGALIAVTLVVWMEDNKGWQWGF
Sbjct: 181 GIKGALPPHGAEQFDETSFEGRKKRSVFFNYFIFCLSCGALIAVTLVVWMEDNKGWQWGF 240

Query: 241 GISTLTILISIPIFLLGSPTYRIKTPAGSPITTIFKVLASAAFNSRKAKTCNNVVMNIST 300
           GISTLTILISIPIFLLGSPTYRIKTPAGSPITTIFKVL SAAFNSRK KT NNVV+NIST
Sbjct: 241 GISTLTILISIPIFLLGSPTYRIKTPAGSPITTIFKVLVSAAFNSRKKKTRNNVVINIST 300

Query: 301 SSVSSDTTTDIILDEQRQNMEKENNPITIPIPVSTQAMEFLNKATINNPNHPQLNCTVKQ 360
           SSVSSD TTDII+DEQRQ +EKEN+    PIP+ TQAMEFLNKATI+NPNHPQLNCTVKQ
Sbjct: 301 SSVSSDATTDIIVDEQRQTLEKEND----PIPIQTQAMEFLNKATIDNPNHPQLNCTVKQ 360

Query: 361 VEEAKIVLKIFPIFTSTIMLNCCLAQLSTFSVQQAATMNTKLGSFKVPPASLPVFPVIFI 420
           VEEAKIVLK+ PIFTSTIMLNCCLAQLSTFSVQQAATMNTKLGS KVPPASLPVFPV+FI
Sbjct: 361 VEEAKIVLKVLPIFTSTIMLNCCLAQLSTFSVQQAATMNTKLGSLKVPPASLPVFPVLFI 420

Query: 421 MILAPTYNHFILPIARKLTKSEMGITHLQRIGTGLVLSTVAMAVAALIETKRKTLAMKIH 480
           MILAPTYNH +LPIARKLTKSEMGITHLQRIGTGLVLSTVAMAVAAL+ETKRK LA+K H
Sbjct: 421 MILAPTYNHLVLPIARKLTKSEMGITHLQRIGTGLVLSTVAMAVAALVETKRKNLAIKTH 480

Query: 481 KLDSSEPLPITFLWVALQYLFLGSADLFSLAGMMEFFFTEAPLSMRSLATALSWASLAMG 540
           KLDS +PLPITFLWVALQYLFLGSADLFSLAGMMEFFFTEAPLSMRSLATALSWASLAMG
Sbjct: 481 KLDSPDPLPITFLWVALQYLFLGSADLFSLAGMMEFFFTEAPLSMRSLATALSWASLAMG 540

Query: 541 YFLSSVLVTLVNGVTKACGFRPWLYGKSLNRYHLERFYWVMCVLSGLNFFQYLFWANRYT 600
           Y+ SSVLVTLVNGVTKACG RPWLYGKSLN YHLERFYWVMC+LSGLNF QYLFWA+RYT
Sbjct: 541 YYFSSVLVTLVNGVTKACGLRPWLYGKSLNHYHLERFYWVMCILSGLNFLQYLFWASRYT 600

Query: 601 YRSTNGGEQSLKEQPNNAALVDERDV 621
           YRS NGGEQSLKEQP  + L++ER+V
Sbjct: 601 YRSINGGEQSLKEQPYKSDLIEEREV 618

BLAST of Lsi06G014860 vs. ExPASy TrEMBL
Match: L0ELE8 (Nitrate transporter NRT1.2B OS=Cucumis sativus OX=3659 PE=2 SV=1)

HSP 1 Score: 1065.8 bits (2755), Expect = 6.6e-308
Identity = 551/626 (88.02%), Postives = 578/626 (92.33%), Query Frame = 0

Query: 1   MSSITVTPLETQNHVEETQQLNLWEDYVDWRKRPAVKGRHGGMLAASFVLVVEVLENLAF 60
           MSS T TPLET+ HVEE Q+LNLWEDYVDWRKRPAVKGRHGGMLAASFVLVVEVLENLAF
Sbjct: 1   MSSTTATPLETEKHVEEAQRLNLWEDYVDWRKRPAVKGRHGGMLAASFVLVVEVLENLAF 60

Query: 61  LANASNLVLYLSKFMHYSPSESANIVTNFMGTAFLLALLGGFLADAFFTTYSIFLISAAI 120
           LANASNLVLYLSKFMHYSPSESANIVTNFMGTAFLLALLGGFL+DAFFTTYSIFLISAAI
Sbjct: 61  LANASNLVLYLSKFMHYSPSESANIVTNFMGTAFLLALLGGFLSDAFFTTYSIFLISAAI 120

Query: 121 ESLG------QAGCPVRLVKVRPARCVTNAAGNGNPCHKVGGGEAVMLFAGLYMVALGVG 180
           ESLG      QA  P     ++P+RCV+NA GNGNPCHKV GGEA MLFAGLYMVALGVG
Sbjct: 121 ESLGLVILTLQADVPY----LKPSRCVSNAVGNGNPCHKVEGGEAAMLFAGLYMVALGVG 180

Query: 181 GIKGSLPPHGAEQFDETSFEGRKKRSAFFNYFIFCLSCGALIAVTLVVWMEDNKGWQWGF 240
           GIKG+LPPHGAEQFDETSFEGRKKRS FFNYFIFCLSCGALIAVTLVVWMEDNKGWQWGF
Sbjct: 181 GIKGALPPHGAEQFDETSFEGRKKRSVFFNYFIFCLSCGALIAVTLVVWMEDNKGWQWGF 240

Query: 241 GISTLTILISIPIFLLGSPTYRIKTPAGSPITTIFKVLASAAFNSRKAKTCNNVVMNIST 300
           GISTLTILISIPIFLLGSPTYRIKTPAGSPITTIFKVL SAAFN+RK KT NNVVMNIST
Sbjct: 241 GISTLTILISIPIFLLGSPTYRIKTPAGSPITTIFKVLVSAAFNNRKKKTRNNVVMNIST 300

Query: 301 SSVSSDTTTDIILDEQRQNMEKENNPITIPIPVSTQAMEFLNKATINNPNHPQLNCTVKQ 360
           SSVSSDTTTDII DEQRQ MEKE++    P P+ TQAMEFLNKATI+NP+HPQLNCTVKQ
Sbjct: 301 SSVSSDTTTDIIADEQRQTMEKESD----PSPIQTQAMEFLNKATIDNPSHPQLNCTVKQ 360

Query: 361 VEEAKIVLKIFPIFTSTIMLNCCLAQLSTFSVQQAATMNTKLGSFKVPPASLPVFPVIFI 420
           VEEAKIVLKI PIFTSTIMLNCCLAQLSTFSVQQAATMNTKLGS KVPPASLPVFPV+FI
Sbjct: 361 VEEAKIVLKILPIFTSTIMLNCCLAQLSTFSVQQAATMNTKLGSLKVPPASLPVFPVLFI 420

Query: 421 MILAPTYNHFILPIARKLTKSEMGITHLQRIGTGLVLSTVAMAVAALIETKRKTLAMKIH 480
           MILAP+YNH +LP+ARKLTKSEMGITHLQRIGTGLVLSTVAMAVAAL+ETKRK LA+K H
Sbjct: 421 MILAPSYNHLVLPVARKLTKSEMGITHLQRIGTGLVLSTVAMAVAALVETKRKNLAIKTH 480

Query: 481 KLDSSEPLPITFLWVALQYLFLGSADLFSLAGMMEFFFTEAPLSMRSLATALSWASLAMG 540
           KLDS +PLPITFLWVALQYLFLGSADLFSLAGMMEFFFTEAPLSMRSLATALSWASLAMG
Sbjct: 481 KLDSPDPLPITFLWVALQYLFLGSADLFSLAGMMEFFFTEAPLSMRSLATALSWASLAMG 540

Query: 541 YFLSSVLVTLVNGVTKACGFRPWLYGKSLNRYHLERFYWVMCVLSGLNFFQYLFWANRYT 600
           Y+ SSVLV LVNGVTKACG RPWL GKSLN YHLERFYWVMC+LSGLNF QYLFWA+RYT
Sbjct: 541 YYFSSVLVALVNGVTKACGLRPWLSGKSLNHYHLERFYWVMCILSGLNFLQYLFWASRYT 600

Query: 601 YRSTNGGEQSLKEQPNNAALVDERDV 621
           YRS NGGEQSLKEQP  + LV+ER+V
Sbjct: 601 YRSINGGEQSLKEQPYKSDLVEEREV 618

BLAST of Lsi06G014860 vs. ExPASy TrEMBL
Match: A0A0A0LP21 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G008560 PE=3 SV=1)

HSP 1 Score: 1064.7 bits (2752), Expect = 1.5e-307
Identity = 550/626 (87.86%), Postives = 578/626 (92.33%), Query Frame = 0

Query: 1   MSSITVTPLETQNHVEETQQLNLWEDYVDWRKRPAVKGRHGGMLAASFVLVVEVLENLAF 60
           MSS T TPLET+ HVEE Q+LNLWEDYVDWRKRPAVKGRHGGMLAASFVLVVEVLENLAF
Sbjct: 1   MSSTTATPLETEKHVEEAQRLNLWEDYVDWRKRPAVKGRHGGMLAASFVLVVEVLENLAF 60

Query: 61  LANASNLVLYLSKFMHYSPSESANIVTNFMGTAFLLALLGGFLADAFFTTYSIFLISAAI 120
           LANASNLVLYLSKFMHYSPSESANIVTNFMGTAFLLALLGGFL+DAFFTTYSIFLISAAI
Sbjct: 61  LANASNLVLYLSKFMHYSPSESANIVTNFMGTAFLLALLGGFLSDAFFTTYSIFLISAAI 120

Query: 121 ESLG------QAGCPVRLVKVRPARCVTNAAGNGNPCHKVGGGEAVMLFAGLYMVALGVG 180
           ESLG      QA  P     ++P+RCV+NA GNGNPCHKV GGEA MLFAGLYMVALGVG
Sbjct: 121 ESLGLVILTLQADVPY----LKPSRCVSNAVGNGNPCHKVEGGEAAMLFAGLYMVALGVG 180

Query: 181 GIKGSLPPHGAEQFDETSFEGRKKRSAFFNYFIFCLSCGALIAVTLVVWMEDNKGWQWGF 240
           GIKG+LPPHGAEQFDETSFEGRKKRS FFNYFIFCLSCGALIAVTLVVWMEDNKGWQWGF
Sbjct: 181 GIKGALPPHGAEQFDETSFEGRKKRSVFFNYFIFCLSCGALIAVTLVVWMEDNKGWQWGF 240

Query: 241 GISTLTILISIPIFLLGSPTYRIKTPAGSPITTIFKVLASAAFNSRKAKTCNNVVMNIST 300
           GISTLTILISIPIFLLGSPTYRIKTPAGSPITTIFKVL SAAFN+RK KT NNVVMNIST
Sbjct: 241 GISTLTILISIPIFLLGSPTYRIKTPAGSPITTIFKVLVSAAFNNRKKKTRNNVVMNIST 300

Query: 301 SSVSSDTTTDIILDEQRQNMEKENNPITIPIPVSTQAMEFLNKATINNPNHPQLNCTVKQ 360
           SSVSSDTTTDII DEQRQ MEKE++    P P+ TQAMEFLNKATI+NP+HPQLNCTVKQ
Sbjct: 301 SSVSSDTTTDIIADEQRQTMEKESD----PSPIQTQAMEFLNKATIDNPSHPQLNCTVKQ 360

Query: 361 VEEAKIVLKIFPIFTSTIMLNCCLAQLSTFSVQQAATMNTKLGSFKVPPASLPVFPVIFI 420
           VEEAKIVLKI PIFTSTIMLNCCLAQLSTFSVQQAATMNTKLGS KVPPASLPVFPV+FI
Sbjct: 361 VEEAKIVLKILPIFTSTIMLNCCLAQLSTFSVQQAATMNTKLGSLKVPPASLPVFPVLFI 420

Query: 421 MILAPTYNHFILPIARKLTKSEMGITHLQRIGTGLVLSTVAMAVAALIETKRKTLAMKIH 480
           MILAP+YNH +LP+ARKLTKSEMGITHLQRIGTGLVLSTVAMAVAAL+ETKRK LA+K H
Sbjct: 421 MILAPSYNHLVLPVARKLTKSEMGITHLQRIGTGLVLSTVAMAVAALVETKRKNLAIKTH 480

Query: 481 KLDSSEPLPITFLWVALQYLFLGSADLFSLAGMMEFFFTEAPLSMRSLATALSWASLAMG 540
           KLDS +PLPITFLWVALQYLFLGSADLFSLAGMMEFFFTEAPLSMRSLATALSWASLAMG
Sbjct: 481 KLDSPDPLPITFLWVALQYLFLGSADLFSLAGMMEFFFTEAPLSMRSLATALSWASLAMG 540

Query: 541 YFLSSVLVTLVNGVTKACGFRPWLYGKSLNRYHLERFYWVMCVLSGLNFFQYLFWANRYT 600
           Y+ SSVLV LVNGVTKACG RPWL GKSLN YHLERFYWVMC+LSGLNF QYLFWA+RYT
Sbjct: 541 YYFSSVLVALVNGVTKACGLRPWLSGKSLNHYHLERFYWVMCILSGLNFLQYLFWASRYT 600

Query: 601 YRSTNGGEQSLKEQPNNAALVDERDV 621
           YRS NGGEQSLK+QP  + LV+ER+V
Sbjct: 601 YRSINGGEQSLKDQPYKSDLVEEREV 618

BLAST of Lsi06G014860 vs. ExPASy TrEMBL
Match: A0A5D3BH75 (Protein NRT1/ PTR FAMILY 4.6-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold350G002360 PE=3 SV=1)

HSP 1 Score: 1050.4 bits (2715), Expect = 2.9e-303
Identity = 544/612 (88.89%), Postives = 568/612 (92.81%), Query Frame = 0

Query: 15  VEETQQLNLWEDYVDWRKRPAVKGRHGGMLAASFVLVVEVLENLAFLANASNLVLYLSKF 74
           +EETQ+LNL EDYVDWRKRPAVKGRHGGMLAASFVLVVEVLENLAFLANASNLVLYLSKF
Sbjct: 79  LEETQRLNLCEDYVDWRKRPAVKGRHGGMLAASFVLVVEVLENLAFLANASNLVLYLSKF 138

Query: 75  MHYSPSESANIVTNFMGTAFLLALLGGFLADAFFTTYSIFLISAAIESLG------QAGC 134
           MHYSPSESANIVTNFMGTAFLLALLGGFLADAFFTTYSIFLISAAIESLG      QA  
Sbjct: 139 MHYSPSESANIVTNFMGTAFLLALLGGFLADAFFTTYSIFLISAAIESLGLVILTLQADV 198

Query: 135 PVRLVKVRPARCVTNAAGNGNPCHKVGGGEAVMLFAGLYMVALGVGGIKGSLPPHGAEQF 194
           P     ++PARCV+NA GNGNPCHKV GGEA MLFAGLYMVALGVGGIKG+LPPHGAEQF
Sbjct: 199 P----HLKPARCVSNAVGNGNPCHKVEGGEAAMLFAGLYMVALGVGGIKGALPPHGAEQF 258

Query: 195 DETSFEGRKKRSAFFNYFIFCLSCGALIAVTLVVWMEDNKGWQWGFGISTLTILISIPIF 254
           DETSFEGRKKRS FFNYFIFCLSCGALIAVTLVVWMEDNKGWQWGFGISTLTILISIPIF
Sbjct: 259 DETSFEGRKKRSVFFNYFIFCLSCGALIAVTLVVWMEDNKGWQWGFGISTLTILISIPIF 318

Query: 255 LLGSPTYRIKTPAGSPITTIFKVLASAAFNSRKAKTCNNVVMNISTSSVSSDTTTDIILD 314
           LLGSPTYRIKTPAGSPITTIFKVL SAAFNSRK KT NNVV+NISTSSVSSD T DII+D
Sbjct: 319 LLGSPTYRIKTPAGSPITTIFKVLVSAAFNSRKKKTRNNVVINISTSSVSSDATADIIVD 378

Query: 315 EQRQNMEKENNPITIPIPVSTQAMEFLNKATINNPNHPQLNCTVKQVEEAKIVLKIFPIF 374
           EQRQ +EKEN+    PIP+ TQAMEFLNKATI+NPNHPQLNCTVKQVEEAKIVLKI PIF
Sbjct: 379 EQRQTLEKEND----PIPIQTQAMEFLNKATIDNPNHPQLNCTVKQVEEAKIVLKILPIF 438

Query: 375 TSTIMLNCCLAQLSTFSVQQAATMNTKLGSFKVPPASLPVFPVIFIMILAPTYNHFILPI 434
           TSTIMLNCCLAQLSTFSVQQAATMNTKLGS KVPPASLPVFPV+FIMILAPTYNH +LPI
Sbjct: 439 TSTIMLNCCLAQLSTFSVQQAATMNTKLGSLKVPPASLPVFPVLFIMILAPTYNHLVLPI 498

Query: 435 ARKLTKSEMGITHLQRIGTGLVLSTVAMAVAALIETKRKTLAMKIHKLDSSEPLPITFLW 494
           ARKLTKSEMGITHLQRIGTGLVLSTVAMAVAAL+ETKRK LA+K HKLDS +PLPITFLW
Sbjct: 499 ARKLTKSEMGITHLQRIGTGLVLSTVAMAVAALVETKRKNLAIKTHKLDSPDPLPITFLW 558

Query: 495 VALQYLFLGSADLFSLAGMMEFFFTEAPLSMRSLATALSWASLAMGYFLSSVLVTLVNGV 554
           VALQYLFLGSADLFSLAGMMEFFFTEAPLSMRSLATALSWASLAMGY+ SSVLVTLVN V
Sbjct: 559 VALQYLFLGSADLFSLAGMMEFFFTEAPLSMRSLATALSWASLAMGYYFSSVLVTLVNDV 618

Query: 555 TKACGFRPWLYGKSLNRYHLERFYWVMCVLSGLNFFQYLFWANRYTYRSTNGGEQSLKEQ 614
           TKACG RPWLYGKSLN YHLERFYWVMC+LSGLNF QYLFWA+RYTYRS NGGEQSLKEQ
Sbjct: 619 TKACGLRPWLYGKSLNHYHLERFYWVMCILSGLNFLQYLFWASRYTYRSINGGEQSLKEQ 678

Query: 615 PNNAALVDERDV 621
           P  + L++ER+V
Sbjct: 679 PYKSDLIEEREV 682

BLAST of Lsi06G014860 vs. NCBI nr
Match: XP_038878388.1 (protein NRT1/ PTR FAMILY 4.6-like isoform X1 [Benincasa hispida])

HSP 1 Score: 1076.6 bits (2783), Expect = 0.0e+00
Identity = 563/626 (89.94%), Postives = 579/626 (92.49%), Query Frame = 0

Query: 1   MSSITVTPLETQNHVEETQQLNLWEDYVDWRKRPAVKGRHGGMLAASFVLVVEVLENLAF 60
           MSSI VTPLE Q H+EETQQLN+W DY DWRKRPAVKGRHGGMLAASFVLVVEVLENLAF
Sbjct: 1   MSSIIVTPLEAQEHMEETQQLNIWGDYFDWRKRPAVKGRHGGMLAASFVLVVEVLENLAF 60

Query: 61  LANASNLVLYLSKFMHYSPSESANIVTNFMGTAFLLALLGGFLADAFFTTYSIFLISAAI 120
           LANASNLVLYLSKFMHYSPSESANIVTNFMGTAFLLALLGGFLADAFFTTYSIFLISAAI
Sbjct: 61  LANASNLVLYLSKFMHYSPSESANIVTNFMGTAFLLALLGGFLADAFFTTYSIFLISAAI 120

Query: 121 ESLG------QAGCPVRLVKVRPARCVTNAAGNGNPCHKVGGGEAVMLFAGLYMVALGVG 180
           ESLG      QA  P     ++PARC+    GNGNPCHKVGGGEA MLFAGLYMVALGVG
Sbjct: 121 ESLGLVILTLQADVPY----LKPARCI---VGNGNPCHKVGGGEAAMLFAGLYMVALGVG 180

Query: 181 GIKGSLPPHGAEQFDETSFEGRKKRSAFFNYFIFCLSCGALIAVTLVVWMEDNKGWQWGF 240
           GIKG+LP HGAEQFDET+FEGRKKRSAFFNYFIFCLSCGALIAVTLVVWMEDNKGWQWGF
Sbjct: 181 GIKGALPSHGAEQFDETTFEGRKKRSAFFNYFIFCLSCGALIAVTLVVWMEDNKGWQWGF 240

Query: 241 GISTLTILISIPIFLLGSPTYRIKTPAGSPITTIFKVLASAAFNSRKAKTCNNVVMNIST 300
           GISTLTILISIPIFLLGSPTYRIKTPAGSPITTIFKVLASAAFN+RKAKT NNVVMN+ST
Sbjct: 241 GISTLTILISIPIFLLGSPTYRIKTPAGSPITTIFKVLASAAFNNRKAKTSNNVVMNLST 300

Query: 301 SSVSSDTTTDIILDEQRQNMEKENNPITIPIPVSTQAMEFLNKATINNPNHPQLNCTVKQ 360
           SSVSSDTTTDII DEQ Q+MEKENN    PIP+ TQ+MEFLNKATINNPNHPQLNCTVKQ
Sbjct: 301 SSVSSDTTTDIIADEQNQSMEKENN----PIPIQTQSMEFLNKATINNPNHPQLNCTVKQ 360

Query: 361 VEEAKIVLKIFPIFTSTIMLNCCLAQLSTFSVQQAATMNTKLGSFKVPPASLPVFPVIFI 420
           VEEAKIVLKIFPIFTSTIMLNCCLAQLSTFSVQQAATMNTKL SFKVPPASLPVFPVIFI
Sbjct: 361 VEEAKIVLKIFPIFTSTIMLNCCLAQLSTFSVQQAATMNTKLSSFKVPPASLPVFPVIFI 420

Query: 421 MILAPTYNHFILPIARKLTKSEMGITHLQRIGTGLVLSTVAMAVAALIETKRKTLAMKIH 480
           MILAPTYN  ILPIARKLTKSEMGITHLQRIGTGLVLSTVAMAVAAL+ETKRK LAMK H
Sbjct: 421 MILAPTYNQLILPIARKLTKSEMGITHLQRIGTGLVLSTVAMAVAALVETKRKALAMKTH 480

Query: 481 KLDSSEPLPITFLWVALQYLFLGSADLFSLAGMMEFFFTEAPLSMRSLATALSWASLAMG 540
           KLDSSEPLPITFLWVALQYLFLGSADLFSLAGMMEFFFTEAPLSMRSLATALSWASLAMG
Sbjct: 481 KLDSSEPLPITFLWVALQYLFLGSADLFSLAGMMEFFFTEAPLSMRSLATALSWASLAMG 540

Query: 541 YFLSSVLVTLVNGVTKACGFRPWLYGKSLNRYHLERFYWVMCVLSGLNFFQYLFWANRYT 600
           Y+ SSVLVTLVNGVTKACG RPWLYGKSLN YHLERFYWVMCVLSGLNF QYLFWANRYT
Sbjct: 541 YYFSSVLVTLVNGVTKACGLRPWLYGKSLNHYHLERFYWVMCVLSGLNFLQYLFWANRYT 600

Query: 601 YRSTNGGEQSLKEQPNNAALVDERDV 621
           YRS NG EQSLKEQ   + LV+ERDV
Sbjct: 601 YRSINGREQSLKEQSQMSDLVEERDV 615

BLAST of Lsi06G014860 vs. NCBI nr
Match: XP_008453176.1 (PREDICTED: protein NRT1/ PTR FAMILY 4.6-like [Cucumis melo] >KAA0057925.1 protein NRT1/ PTR FAMILY 4.6-like [Cucumis melo var. makuwa])

HSP 1 Score: 1076.2 bits (2782), Expect = 0.0e+00
Identity = 556/626 (88.82%), Postives = 582/626 (92.97%), Query Frame = 0

Query: 1   MSSITVTPLETQNHVEETQQLNLWEDYVDWRKRPAVKGRHGGMLAASFVLVVEVLENLAF 60
           MSSIT TPL+T+ HVEETQ+LNL EDYVDWRKRPAVKGRHGGMLAASFVLVVEVLENLAF
Sbjct: 1   MSSITATPLQTEKHVEETQRLNLCEDYVDWRKRPAVKGRHGGMLAASFVLVVEVLENLAF 60

Query: 61  LANASNLVLYLSKFMHYSPSESANIVTNFMGTAFLLALLGGFLADAFFTTYSIFLISAAI 120
           LANASNLVLYLSKFMHYSPSESANIVTNFMGTAFLLALLGGFLADAFFTTYSIFLISAAI
Sbjct: 61  LANASNLVLYLSKFMHYSPSESANIVTNFMGTAFLLALLGGFLADAFFTTYSIFLISAAI 120

Query: 121 ESLG------QAGCPVRLVKVRPARCVTNAAGNGNPCHKVGGGEAVMLFAGLYMVALGVG 180
           ESLG      QA  P     ++PARCV+NA GNGNPCHKV GGEA MLFAGLYMVALGVG
Sbjct: 121 ESLGLVILTLQADVP----HLKPARCVSNAVGNGNPCHKVEGGEAAMLFAGLYMVALGVG 180

Query: 181 GIKGSLPPHGAEQFDETSFEGRKKRSAFFNYFIFCLSCGALIAVTLVVWMEDNKGWQWGF 240
           GIKG+LPPHGAEQFDETSFEGRKKRS FFNYFIFCLSCGALIAVTLVVWMEDNKGWQWGF
Sbjct: 181 GIKGALPPHGAEQFDETSFEGRKKRSVFFNYFIFCLSCGALIAVTLVVWMEDNKGWQWGF 240

Query: 241 GISTLTILISIPIFLLGSPTYRIKTPAGSPITTIFKVLASAAFNSRKAKTCNNVVMNIST 300
           GISTLTILISIPIFLLGSPTYRIKTPAGSPITTIFKVL SAAFNSRK KT NNVV+NIST
Sbjct: 241 GISTLTILISIPIFLLGSPTYRIKTPAGSPITTIFKVLVSAAFNSRKKKTRNNVVINIST 300

Query: 301 SSVSSDTTTDIILDEQRQNMEKENNPITIPIPVSTQAMEFLNKATINNPNHPQLNCTVKQ 360
           SSVSSD TTDII+DEQRQ +EKEN+    PIP+ TQAMEFLNKATI+NPNHPQLNCTVKQ
Sbjct: 301 SSVSSDATTDIIVDEQRQTLEKEND----PIPIQTQAMEFLNKATIDNPNHPQLNCTVKQ 360

Query: 361 VEEAKIVLKIFPIFTSTIMLNCCLAQLSTFSVQQAATMNTKLGSFKVPPASLPVFPVIFI 420
           VEEAKIVLK+ PIFTSTIMLNCCLAQLSTFSVQQAATMNTKLGS KVPPASLPVFPV+FI
Sbjct: 361 VEEAKIVLKVLPIFTSTIMLNCCLAQLSTFSVQQAATMNTKLGSLKVPPASLPVFPVLFI 420

Query: 421 MILAPTYNHFILPIARKLTKSEMGITHLQRIGTGLVLSTVAMAVAALIETKRKTLAMKIH 480
           MILAPTYNH +LPIARKLTKSEMGITHLQRIGTGLVLSTVAMAVAAL+ETKRK LA+K H
Sbjct: 421 MILAPTYNHLVLPIARKLTKSEMGITHLQRIGTGLVLSTVAMAVAALVETKRKNLAIKTH 480

Query: 481 KLDSSEPLPITFLWVALQYLFLGSADLFSLAGMMEFFFTEAPLSMRSLATALSWASLAMG 540
           KLDS +PLPITFLWVALQYLFLGSADLFSLAGMMEFFFTEAPLSMRSLATALSWASLAMG
Sbjct: 481 KLDSPDPLPITFLWVALQYLFLGSADLFSLAGMMEFFFTEAPLSMRSLATALSWASLAMG 540

Query: 541 YFLSSVLVTLVNGVTKACGFRPWLYGKSLNRYHLERFYWVMCVLSGLNFFQYLFWANRYT 600
           Y+ SSVLVTLVNGVTKACG RPWLYGKSLN YHLERFYWVMC+LSGLNF QYLFWA+RYT
Sbjct: 541 YYFSSVLVTLVNGVTKACGLRPWLYGKSLNHYHLERFYWVMCILSGLNFLQYLFWASRYT 600

Query: 601 YRSTNGGEQSLKEQPNNAALVDERDV 621
           YRS NGGEQSLKEQP  + L++ER+V
Sbjct: 601 YRSINGGEQSLKEQPYKSDLIEEREV 618

BLAST of Lsi06G014860 vs. NCBI nr
Match: NP_001267691.1 (nitrate transporter 1.2-like [Cucumis sativus] >AGA60120.1 nitrate transporter NRT1.2B [Cucumis sativus] >KAE8652411.1 hypothetical protein Csa_013073 [Cucumis sativus])

HSP 1 Score: 1065.8 bits (2755), Expect = 1.4e-307
Identity = 551/626 (88.02%), Postives = 578/626 (92.33%), Query Frame = 0

Query: 1   MSSITVTPLETQNHVEETQQLNLWEDYVDWRKRPAVKGRHGGMLAASFVLVVEVLENLAF 60
           MSS T TPLET+ HVEE Q+LNLWEDYVDWRKRPAVKGRHGGMLAASFVLVVEVLENLAF
Sbjct: 1   MSSTTATPLETEKHVEEAQRLNLWEDYVDWRKRPAVKGRHGGMLAASFVLVVEVLENLAF 60

Query: 61  LANASNLVLYLSKFMHYSPSESANIVTNFMGTAFLLALLGGFLADAFFTTYSIFLISAAI 120
           LANASNLVLYLSKFMHYSPSESANIVTNFMGTAFLLALLGGFL+DAFFTTYSIFLISAAI
Sbjct: 61  LANASNLVLYLSKFMHYSPSESANIVTNFMGTAFLLALLGGFLSDAFFTTYSIFLISAAI 120

Query: 121 ESLG------QAGCPVRLVKVRPARCVTNAAGNGNPCHKVGGGEAVMLFAGLYMVALGVG 180
           ESLG      QA  P     ++P+RCV+NA GNGNPCHKV GGEA MLFAGLYMVALGVG
Sbjct: 121 ESLGLVILTLQADVPY----LKPSRCVSNAVGNGNPCHKVEGGEAAMLFAGLYMVALGVG 180

Query: 181 GIKGSLPPHGAEQFDETSFEGRKKRSAFFNYFIFCLSCGALIAVTLVVWMEDNKGWQWGF 240
           GIKG+LPPHGAEQFDETSFEGRKKRS FFNYFIFCLSCGALIAVTLVVWMEDNKGWQWGF
Sbjct: 181 GIKGALPPHGAEQFDETSFEGRKKRSVFFNYFIFCLSCGALIAVTLVVWMEDNKGWQWGF 240

Query: 241 GISTLTILISIPIFLLGSPTYRIKTPAGSPITTIFKVLASAAFNSRKAKTCNNVVMNIST 300
           GISTLTILISIPIFLLGSPTYRIKTPAGSPITTIFKVL SAAFN+RK KT NNVVMNIST
Sbjct: 241 GISTLTILISIPIFLLGSPTYRIKTPAGSPITTIFKVLVSAAFNNRKKKTRNNVVMNIST 300

Query: 301 SSVSSDTTTDIILDEQRQNMEKENNPITIPIPVSTQAMEFLNKATINNPNHPQLNCTVKQ 360
           SSVSSDTTTDII DEQRQ MEKE++    P P+ TQAMEFLNKATI+NP+HPQLNCTVKQ
Sbjct: 301 SSVSSDTTTDIIADEQRQTMEKESD----PSPIQTQAMEFLNKATIDNPSHPQLNCTVKQ 360

Query: 361 VEEAKIVLKIFPIFTSTIMLNCCLAQLSTFSVQQAATMNTKLGSFKVPPASLPVFPVIFI 420
           VEEAKIVLKI PIFTSTIMLNCCLAQLSTFSVQQAATMNTKLGS KVPPASLPVFPV+FI
Sbjct: 361 VEEAKIVLKILPIFTSTIMLNCCLAQLSTFSVQQAATMNTKLGSLKVPPASLPVFPVLFI 420

Query: 421 MILAPTYNHFILPIARKLTKSEMGITHLQRIGTGLVLSTVAMAVAALIETKRKTLAMKIH 480
           MILAP+YNH +LP+ARKLTKSEMGITHLQRIGTGLVLSTVAMAVAAL+ETKRK LA+K H
Sbjct: 421 MILAPSYNHLVLPVARKLTKSEMGITHLQRIGTGLVLSTVAMAVAALVETKRKNLAIKTH 480

Query: 481 KLDSSEPLPITFLWVALQYLFLGSADLFSLAGMMEFFFTEAPLSMRSLATALSWASLAMG 540
           KLDS +PLPITFLWVALQYLFLGSADLFSLAGMMEFFFTEAPLSMRSLATALSWASLAMG
Sbjct: 481 KLDSPDPLPITFLWVALQYLFLGSADLFSLAGMMEFFFTEAPLSMRSLATALSWASLAMG 540

Query: 541 YFLSSVLVTLVNGVTKACGFRPWLYGKSLNRYHLERFYWVMCVLSGLNFFQYLFWANRYT 600
           Y+ SSVLV LVNGVTKACG RPWL GKSLN YHLERFYWVMC+LSGLNF QYLFWA+RYT
Sbjct: 541 YYFSSVLVALVNGVTKACGLRPWLSGKSLNHYHLERFYWVMCILSGLNFLQYLFWASRYT 600

Query: 601 YRSTNGGEQSLKEQPNNAALVDERDV 621
           YRS NGGEQSLKEQP  + LV+ER+V
Sbjct: 601 YRSINGGEQSLKEQPYKSDLVEEREV 618

BLAST of Lsi06G014860 vs. NCBI nr
Match: TYJ98613.1 (protein NRT1/ PTR FAMILY 4.6-like [Cucumis melo var. makuwa])

HSP 1 Score: 1050.4 bits (2715), Expect = 6.0e-303
Identity = 544/612 (88.89%), Postives = 568/612 (92.81%), Query Frame = 0

Query: 15  VEETQQLNLWEDYVDWRKRPAVKGRHGGMLAASFVLVVEVLENLAFLANASNLVLYLSKF 74
           +EETQ+LNL EDYVDWRKRPAVKGRHGGMLAASFVLVVEVLENLAFLANASNLVLYLSKF
Sbjct: 79  LEETQRLNLCEDYVDWRKRPAVKGRHGGMLAASFVLVVEVLENLAFLANASNLVLYLSKF 138

Query: 75  MHYSPSESANIVTNFMGTAFLLALLGGFLADAFFTTYSIFLISAAIESLG------QAGC 134
           MHYSPSESANIVTNFMGTAFLLALLGGFLADAFFTTYSIFLISAAIESLG      QA  
Sbjct: 139 MHYSPSESANIVTNFMGTAFLLALLGGFLADAFFTTYSIFLISAAIESLGLVILTLQADV 198

Query: 135 PVRLVKVRPARCVTNAAGNGNPCHKVGGGEAVMLFAGLYMVALGVGGIKGSLPPHGAEQF 194
           P     ++PARCV+NA GNGNPCHKV GGEA MLFAGLYMVALGVGGIKG+LPPHGAEQF
Sbjct: 199 P----HLKPARCVSNAVGNGNPCHKVEGGEAAMLFAGLYMVALGVGGIKGALPPHGAEQF 258

Query: 195 DETSFEGRKKRSAFFNYFIFCLSCGALIAVTLVVWMEDNKGWQWGFGISTLTILISIPIF 254
           DETSFEGRKKRS FFNYFIFCLSCGALIAVTLVVWMEDNKGWQWGFGISTLTILISIPIF
Sbjct: 259 DETSFEGRKKRSVFFNYFIFCLSCGALIAVTLVVWMEDNKGWQWGFGISTLTILISIPIF 318

Query: 255 LLGSPTYRIKTPAGSPITTIFKVLASAAFNSRKAKTCNNVVMNISTSSVSSDTTTDIILD 314
           LLGSPTYRIKTPAGSPITTIFKVL SAAFNSRK KT NNVV+NISTSSVSSD T DII+D
Sbjct: 319 LLGSPTYRIKTPAGSPITTIFKVLVSAAFNSRKKKTRNNVVINISTSSVSSDATADIIVD 378

Query: 315 EQRQNMEKENNPITIPIPVSTQAMEFLNKATINNPNHPQLNCTVKQVEEAKIVLKIFPIF 374
           EQRQ +EKEN+    PIP+ TQAMEFLNKATI+NPNHPQLNCTVKQVEEAKIVLKI PIF
Sbjct: 379 EQRQTLEKEND----PIPIQTQAMEFLNKATIDNPNHPQLNCTVKQVEEAKIVLKILPIF 438

Query: 375 TSTIMLNCCLAQLSTFSVQQAATMNTKLGSFKVPPASLPVFPVIFIMILAPTYNHFILPI 434
           TSTIMLNCCLAQLSTFSVQQAATMNTKLGS KVPPASLPVFPV+FIMILAPTYNH +LPI
Sbjct: 439 TSTIMLNCCLAQLSTFSVQQAATMNTKLGSLKVPPASLPVFPVLFIMILAPTYNHLVLPI 498

Query: 435 ARKLTKSEMGITHLQRIGTGLVLSTVAMAVAALIETKRKTLAMKIHKLDSSEPLPITFLW 494
           ARKLTKSEMGITHLQRIGTGLVLSTVAMAVAAL+ETKRK LA+K HKLDS +PLPITFLW
Sbjct: 499 ARKLTKSEMGITHLQRIGTGLVLSTVAMAVAALVETKRKNLAIKTHKLDSPDPLPITFLW 558

Query: 495 VALQYLFLGSADLFSLAGMMEFFFTEAPLSMRSLATALSWASLAMGYFLSSVLVTLVNGV 554
           VALQYLFLGSADLFSLAGMMEFFFTEAPLSMRSLATALSWASLAMGY+ SSVLVTLVN V
Sbjct: 559 VALQYLFLGSADLFSLAGMMEFFFTEAPLSMRSLATALSWASLAMGYYFSSVLVTLVNDV 618

Query: 555 TKACGFRPWLYGKSLNRYHLERFYWVMCVLSGLNFFQYLFWANRYTYRSTNGGEQSLKEQ 614
           TKACG RPWLYGKSLN YHLERFYWVMC+LSGLNF QYLFWA+RYTYRS NGGEQSLKEQ
Sbjct: 619 TKACGLRPWLYGKSLNHYHLERFYWVMCILSGLNFLQYLFWASRYTYRSINGGEQSLKEQ 678

Query: 615 PNNAALVDERDV 621
           P  + L++ER+V
Sbjct: 679 PYKSDLIEEREV 682

BLAST of Lsi06G014860 vs. NCBI nr
Match: XP_038878390.1 (protein NRT1/ PTR FAMILY 4.6-like isoform X2 [Benincasa hispida] >XP_038878391.1 protein NRT1/ PTR FAMILY 4.6-like isoform X2 [Benincasa hispida] >XP_038878392.1 protein NRT1/ PTR FAMILY 4.6-like isoform X2 [Benincasa hispida] >XP_038878393.1 protein NRT1/ PTR FAMILY 4.6-like isoform X2 [Benincasa hispida] >XP_038878394.1 protein NRT1/ PTR FAMILY 4.6-like isoform X2 [Benincasa hispida])

HSP 1 Score: 1001.1 bits (2587), Expect = 4.1e-288
Identity = 528/584 (90.41%), Postives = 542/584 (92.81%), Query Frame = 0

Query: 43  MLAASFVLVVEVLENLAFLANASNLVLYLSKFMHYSPSESANIVTNFMGTAFLLALLGGF 102
           MLAASFVLVVEVLENLAFLANASNLVLYLSKFMHYSPSESANIVTNFMGTAFLLALLGGF
Sbjct: 1   MLAASFVLVVEVLENLAFLANASNLVLYLSKFMHYSPSESANIVTNFMGTAFLLALLGGF 60

Query: 103 LADAFFTTYSIFLISAAIESLG------QAGCPVRLVKVRPARCVTNAAGNGNPCHKVGG 162
           LADAFFTTYSIFLISAAIESLG      QA  P     ++PARC+    GNGNPCHKVGG
Sbjct: 61  LADAFFTTYSIFLISAAIESLGLVILTLQADVPY----LKPARCI---VGNGNPCHKVGG 120

Query: 163 GEAVMLFAGLYMVALGVGGIKGSLPPHGAEQFDETSFEGRKKRSAFFNYFIFCLSCGALI 222
           GEA MLFAGLYMVALGVGGIKG+LP HGAEQFDET+FEGRKKRSAFFNYFIFCLSCGALI
Sbjct: 121 GEAAMLFAGLYMVALGVGGIKGALPSHGAEQFDETTFEGRKKRSAFFNYFIFCLSCGALI 180

Query: 223 AVTLVVWMEDNKGWQWGFGISTLTILISIPIFLLGSPTYRIKTPAGSPITTIFKVLASAA 282
           AVTLVVWMEDNKGWQWGFGISTLTILISIPIFLLGSPTYRIKTPAGSPITTIFKVLASAA
Sbjct: 181 AVTLVVWMEDNKGWQWGFGISTLTILISIPIFLLGSPTYRIKTPAGSPITTIFKVLASAA 240

Query: 283 FNSRKAKTCNNVVMNISTSSVSSDTTTDIILDEQRQNMEKENNPITIPIPVSTQAMEFLN 342
           FN+RKAKT NNVVMN+STSSVSSDTTTDII DEQ Q+MEKENN    PIP+ TQ+MEFLN
Sbjct: 241 FNNRKAKTSNNVVMNLSTSSVSSDTTTDIIADEQNQSMEKENN----PIPIQTQSMEFLN 300

Query: 343 KATINNPNHPQLNCTVKQVEEAKIVLKIFPIFTSTIMLNCCLAQLSTFSVQQAATMNTKL 402
           KATINNPNHPQLNCTVKQVEEAKIVLKIFPIFTSTIMLNCCLAQLSTFSVQQAATMNTKL
Sbjct: 301 KATINNPNHPQLNCTVKQVEEAKIVLKIFPIFTSTIMLNCCLAQLSTFSVQQAATMNTKL 360

Query: 403 GSFKVPPASLPVFPVIFIMILAPTYNHFILPIARKLTKSEMGITHLQRIGTGLVLSTVAM 462
            SFKVPPASLPVFPVIFIMILAPTYN  ILPIARKLTKSEMGITHLQRIGTGLVLSTVAM
Sbjct: 361 SSFKVPPASLPVFPVIFIMILAPTYNQLILPIARKLTKSEMGITHLQRIGTGLVLSTVAM 420

Query: 463 AVAALIETKRKTLAMKIHKLDSSEPLPITFLWVALQYLFLGSADLFSLAGMMEFFFTEAP 522
           AVAAL+ETKRK LAMK HKLDSSEPLPITFLWVALQYLFLGSADLFSLAGMMEFFFTEAP
Sbjct: 421 AVAALVETKRKALAMKTHKLDSSEPLPITFLWVALQYLFLGSADLFSLAGMMEFFFTEAP 480

Query: 523 LSMRSLATALSWASLAMGYFLSSVLVTLVNGVTKACGFRPWLYGKSLNRYHLERFYWVMC 582
           LSMRSLATALSWASLAMGY+ SSVLVTLVNGVTKACG RPWLYGKSLN YHLERFYWVMC
Sbjct: 481 LSMRSLATALSWASLAMGYYFSSVLVTLVNGVTKACGLRPWLYGKSLNHYHLERFYWVMC 540

Query: 583 VLSGLNFFQYLFWANRYTYRSTNGGEQSLKEQPNNAALVDERDV 621
           VLSGLNF QYLFWANRYTYRS NG EQSLKEQ   + LV+ERDV
Sbjct: 541 VLSGLNFLQYLFWANRYTYRSINGREQSLKEQSQMSDLVEERDV 573

BLAST of Lsi06G014860 vs. TAIR 10
Match: AT1G69850.1 (nitrate transporter 1:2 )

HSP 1 Score: 709.1 bits (1829), Expect = 3.0e-204
Identity = 371/587 (63.20%), Postives = 450/587 (76.66%), Query Frame = 0

Query: 15  VEETQQLNLWEDYVDWRKRPAVKGRHGGMLAASFVLVVEVLENLAFLANASNLVLYLSKF 74
           +E  ++++ WE Y DWR R AVKGRHGGMLAASFVLVVE+LENLA+LANASNLVLYL ++
Sbjct: 1   MEVEEEVSRWEGYADWRNRAAVKGRHGGMLAASFVLVVEILENLAYLANASNLVLYLREY 60

Query: 75  MHYSPSESANIVTNFMGTAFLLALLGGFLADAFFTTYSIFLISAAIESLGQAGCPV--RL 134
           MH SPS+SAN VTNFMGTAFLLALLGGFL+DAFF+T+ IFLISA+IE LG     +  R 
Sbjct: 61  MHMSPSKSANDVTNFMGTAFLLALLGGFLSDAFFSTFQIFLISASIEFLGLIILTIQART 120

Query: 135 VKVRPARCVTNAAGNGNPCHKVGGGEAVMLFAGLYMVALGVGGIKGSLPPHGAEQFDETS 194
             + P  C      +   C +V G +A MLF GLY+VALGVGGIKGSL  HGAEQFDE++
Sbjct: 121 PSLMPPSC------DSPTCEEVSGSKAAMLFVGLYLVALGVGGIKGSLASHGAEQFDEST 180

Query: 195 FEGRKKRSAFFNYFIFCLSCGALIAVTLVVWMEDNKGWQWGFGISTLTILISIPIFLLGS 254
            +GRK+RS FFNYF+FCL+CGAL+AVT VVW+EDNKGW+WGFG+ST+ I +SI IFL GS
Sbjct: 181 PKGRKQRSTFFNYFVFCLACGALVAVTFVVWLEDNKGWEWGFGVSTIAIFVSILIFLSGS 240

Query: 255 PTYRIKTPAGSPITTIFKVLASAAFNSRKAKTCNNVVMNISTSSVSSDTTTDIILDEQRQ 314
             YR K P GSP+TTI KVL +A+     + + +N V ++S S  +   +      E + 
Sbjct: 241 RFYRNKIPCGSPLTTILKVLLAASVKCCSSGSSSNAVASMSVSPSNHCVSKGKKEVESQG 300

Query: 315 NMEKENNPITIPIPVS-TQAMEFLNKATINNPNHPQLNCTVKQVEEAKIVLKIFPIFTST 374
            +EK      +P     T +++ LN A    P H  L CTV+QVE+ KIVLK+ PIF  T
Sbjct: 301 ELEKPRQEEALPPRAQLTNSLKVLNGAADEKPVHRLLECTVQQVEDVKIVLKMLPIFACT 360

Query: 375 IMLNCCLAQLSTFSVQQAATMNTKLGSFKVPPASLPVFPVIFIMILAPTYNHFILPIARK 434
           IMLNCCLAQLSTFSVQQAA+MNTK+GS K+PPASLP+FPV+FIMILAP Y+H I+P ARK
Sbjct: 361 IMLNCCLAQLSTFSVQQAASMNTKIGSLKIPPASLPIFPVVFIMILAPIYDHLIIPFARK 420

Query: 435 LTKSEMGITHLQRIGTGLVLSTVAMAVAALIETKRKTLAMKIHKLDSSEPLPITFLWVAL 494
            TK+E G+THLQRIG GLVLS +AMAVAAL+E KRK +A     LDS E LP+TFLW+AL
Sbjct: 421 ATKTETGVTHLQRIGVGLVLSILAMAVAALVEIKRKGVAKDSGLLDSKETLPVTFLWIAL 480

Query: 495 QYLFLGSADLFSLAGMMEFFFTEAPLSMRSLATALSWASLAMGYFLSSVLVTLVNGVTKA 554
           QYLFLGSADLF+LAG++E+FFTEAP SMRSLAT+LSWASLAMGY+LSSV+V++VN +T +
Sbjct: 481 QYLFLGSADLFTLAGLLEYFFTEAPSSMRSLATSLSWASLAMGYYLSSVIVSIVNSITGS 540

Query: 555 CGFRPWLYGKSLNRYHLERFYWVMCVLSGLNFFQYLFWANRYTYRST 599
            G  PWL GKS+NRY L+ FYW+MCVLS  NF  YLFWA RY YRST
Sbjct: 541 SGNTPWLRGKSINRYKLDYFYWLMCVLSAANFLHYLFWAMRYKYRST 581

BLAST of Lsi06G014860 vs. TAIR 10
Match: AT5G62730.1 (Major facilitator superfamily protein )

HSP 1 Score: 694.5 bits (1791), Expect = 7.7e-200
Identity = 374/592 (63.18%), Postives = 444/592 (75.00%), Query Frame = 0

Query: 15  VEETQQLNLWEDYVDWRKRPAVKGRHGGMLAASFVLVVEVLENLAFLANASNLVLYLSKF 74
           ++E  +L+ W  YVDWR RPA++GRHGGMLAASFVLVVEVLENLAFLANASNLVLYLS  
Sbjct: 32  MDEANRLSAWNGYVDWRSRPALRGRHGGMLAASFVLVVEVLENLAFLANASNLVLYLSTK 91

Query: 75  MHYSPSESANIVTNFMGTAFLLALLGGFLADAFFTTYSIFLISAAIESLGQAGCPVRLVK 134
           M +SPS +AN VT FMGTAF LALLGGFLADAFFTT+ I+L+SAAIE LG     V+  +
Sbjct: 92  MGFSPSGAANAVTAFMGTAFFLALLGGFLADAFFTTFHIYLVSAAIEFLGLMVLTVQAHE 151

Query: 135 VRPARCVTNAAGNGNPCHKVGGGEAVMLFAGLYMVALGVGGIKGSLPPHGAEQFDETSFE 194
                            H       V LF GLY+VALGVGGIKGSLPPHGAEQFDE +  
Sbjct: 152 -----------------HSTEPWSRVFLFVGLYLVALGVGGIKGSLPPHGAEQFDEETSS 211

Query: 195 GRKKRSAFFNYFIFCLSCGALIAVTLVVWMEDNKGWQWGFGISTLTILISIPIFLLGSPT 254
           GR++RS FFNYFIF LSCGALIAVT+VVW+EDNKGW +GFG+ST  ILIS+P+FL GS  
Sbjct: 212 GRRQRSFFFNYFIFSLSCGALIAVTVVVWLEDNKGWSYGFGVSTAAILISVPVFLAGSRV 271

Query: 255 YRIKTPAGSPITTIFKVLASAAFNSRKAKTCNNVVMNISTSSVSSDTTTDIILDEQRQNM 314
           YR+K P+GSPITT+FKVL +A +   K +  + +V+   T +   D+ T       +QN 
Sbjct: 272 YRLKVPSGSPITTLFKVLTAALYAKYKKRRTSRIVVTCHTRNDCDDSVT-------KQNC 331

Query: 315 EKENNPITIPIPVSTQAMEFLNKATINNPNHPQ-LNCTVKQVEEAKIVLKIFPIFTSTIM 374
           + ++  +            FL +      + P+ L CT +QV++ KIV+KI PIF STIM
Sbjct: 332 DGDDGFLG----------SFLGEVVRERESLPRPLRCTEEQVKDVKIVIKILPIFMSTIM 391

Query: 375 LNCCLAQLSTFSVQQAATMNTKLGSFKVPPASLPVFPVIFIMILAPTYNHFILPIARKLT 434
           LNCCLAQLSTFSVQQA+TMNTKLGSF VPPA+LPVFPV+F+MILAPTYNH +LP+ARK T
Sbjct: 392 LNCCLAQLSTFSVQQASTMNTKLGSFTVPPAALPVFPVVFMMILAPTYNHLLLPLARKST 451

Query: 435 KSEMGITHLQRIGTGLVLSTVAMAVAALIETKRKTLAMKIHKLD-----SSEPLPITFLW 494
           K+E GITHLQRIGTGLVLS VAMAVAAL+ETKRK + +     +     SS PLPITFLW
Sbjct: 452 KTETGITHLQRIGTGLVLSIVAMAVAALVETKRKHVVVSCCSNNNSSSYSSSPLPITFLW 511

Query: 495 VALQYLFLGSADLFSLAGMMEFFFTEAPLSMRSLATALSWASLAMGYFLSSVLVTLVNGV 554
           VA+QY+FLGSADLF+LAGMMEFFFTEAP +MRSLAT+LSWASLAMGY+ SSVLV+ VN V
Sbjct: 512 VAIQYVFLGSADLFTLAGMMEFFFTEAPSTMRSLATSLSWASLAMGYYFSSVLVSAVNFV 571

Query: 555 TKACGFRPWLYGKSLNRYHLERFYWVMCVLSGLNFFQYLFWANRYTYRSTNG 601
           T      PWL G++LN+YHLERFYW+MCVLSG+NF  YLFWA+RY YRS  G
Sbjct: 572 TGLNHHNPWLLGENLNQYHLERFYWLMCVLSGINFLHYLFWASRYVYRSNQG 589

BLAST of Lsi06G014860 vs. TAIR 10
Match: AT1G27040.1 (Major facilitator superfamily protein )

HSP 1 Score: 677.2 bits (1746), Expect = 1.3e-194
Identity = 364/586 (62.12%), Postives = 438/586 (74.74%), Query Frame = 0

Query: 15  VEETQQLNLWEDYVDWRKRPAVKGRHGGMLAASFVLVVEVLENLAFLANASNLVLYLSKF 74
           VE    ++ WE Y DWR + A++GRHGGMLAASFVL VE+LENLAFLANASNLVLYL  F
Sbjct: 3   VEMHGDVSKWEGYADWRNKAALRGRHGGMLAASFVLAVEILENLAFLANASNLVLYLKNF 62

Query: 75  MHYSPSESANIVTNFMGTAFLLALLGGFLADAFFTTYSIFLISAAIESLGQAGCPV--RL 134
           MH S + S++ VT FM TAFLLALLGGFLADAFF+T+ IFLISA+IE LG     +  R 
Sbjct: 63  MHMSLARSSSEVTTFMATAFLLALLGGFLADAFFSTFVIFLISASIEFLGLILLTIQARR 122

Query: 135 VKVRPARCVTNAAGNGNPCHKVGGGEAVMLFAGLYMVALGVGGIKGSLPPHGAEQFDETS 194
             + P  C ++AA     C  VGG +A  LF GLY+V+LG+GGIKGSLP HGAEQFDE +
Sbjct: 123 PSLMPPPCKSSAALR---CEVVGGSKAAFLFVGLYLVSLGIGGIKGSLPSHGAEQFDEGT 182

Query: 195 FEGRKKRSAFFNYFIFCLSCGALIAVTLVVWMEDNKGWQWGFGISTLTILISIPIFLLGS 254
            +GRK+RS FFNY++FCLSCGAL+AVT VVW+EDNKGW+WGFG+ST++I +SI +FLLGS
Sbjct: 183 PKGRKQRSTFFNYYVFCLSCGALVAVTFVVWIEDNKGWEWGFGVSTISIFLSILVFLLGS 242

Query: 255 PTYRIKTPAGSPITTIFKVLASAAFNSRKAKTCNNVVMNISTSSVSSDTTTDIILDEQRQ 314
             Y+ K P GSP+TTIFKVL +A+  S  +KT +N   +    S   + T          
Sbjct: 243 RFYKNKIPRGSPLTTIFKVLLAASIVSCSSKTSSNHFTSREVQSEHEEKT---------- 302

Query: 315 NMEKENNPITIPIPVSTQAMEFLNKATINNPNHPQLNCTVKQVEEAKIVLKIFPIFTSTI 374
                      P    T ++  LNKA     +H  L CTV+QVE+ KIVLK+ PIF  TI
Sbjct: 303 -----------PSQSLTNSLTCLNKAIEGKTHHIWLECTVQQVEDVKIVLKMLPIFGCTI 362

Query: 375 MLNCCLAQLSTFSVQQAATMNTKLGSFKVPPASLPVFPVIFIMILAPTYNHFILPIARKL 434
           MLNCCLAQLST+SV QAATMN K+ +F VP ASLPVFPV+F++ILAPTY+H I+P ARK+
Sbjct: 363 MLNCCLAQLSTYSVHQAATMNRKIVNFNVPSASLPVFPVVFMLILAPTYDHLIIPFARKV 422

Query: 435 TKSEMGITHLQRIGTGLVLSTVAMAVAALIETKRKTLAMKIHKLDSSEPLPITFLWVALQ 494
           TKSE+GITHLQRIG GLVLS VAMAVAAL+E KRK +A +   LDS E LPITFLW+ALQ
Sbjct: 423 TKSEIGITHLQRIGVGLVLSIVAMAVAALVELKRKQVAREAGLLDSEETLPITFLWIALQ 482

Query: 495 YLFLGSADLFSLAGMMEFFFTEAPLSMRSLATALSWASLAMGYFLSSVLVTLVNGVTKAC 554
           YLFLGSADLF+LAG++EFFFTEAP SMRSLAT+LSWASLA+GY+LSSV+V +VN VTK+ 
Sbjct: 483 YLFLGSADLFTLAGLLEFFFTEAPSSMRSLATSLSWASLALGYYLSSVMVPIVNRVTKSA 542

Query: 555 GFRPWLYGKSLNRYHLERFYWVMCVLSGLNFFQYLFWANRYTYRST 599
           G  PWL G+ LNR  L+ FYW+MCVLS +NF  YLFWA RY Y ST
Sbjct: 543 GQSPWL-GEKLNRNRLDLFYWLMCVLSVVNFLHYLFWAKRYKYIST 563

BLAST of Lsi06G014860 vs. TAIR 10
Match: AT1G27040.2 (Major facilitator superfamily protein )

HSP 1 Score: 676.0 bits (1743), Expect = 2.8e-194
Identity = 362/577 (62.74%), Postives = 434/577 (75.22%), Query Frame = 0

Query: 24  WEDYVDWRKRPAVKGRHGGMLAASFVLVVEVLENLAFLANASNLVLYLSKFMHYSPSESA 83
           WE Y DWR + A++GRHGGMLAASFVL VE+LENLAFLANASNLVLYL  FMH S + S+
Sbjct: 8   WEGYADWRNKAALRGRHGGMLAASFVLAVEILENLAFLANASNLVLYLKNFMHMSLARSS 67

Query: 84  NIVTNFMGTAFLLALLGGFLADAFFTTYSIFLISAAIESLGQAGCPV--RLVKVRPARCV 143
           + VT FM TAFLLALLGGFLADAFF+T+ IFLISA+IE LG     +  R   + P  C 
Sbjct: 68  SEVTTFMATAFLLALLGGFLADAFFSTFVIFLISASIEFLGLILLTIQARRPSLMPPPCK 127

Query: 144 TNAAGNGNPCHKVGGGEAVMLFAGLYMVALGVGGIKGSLPPHGAEQFDETSFEGRKKRSA 203
           ++AA     C  VGG +A  LF GLY+V+LG+GGIKGSLP HGAEQFDE + +GRK+RS 
Sbjct: 128 SSAALR---CEVVGGSKAAFLFVGLYLVSLGIGGIKGSLPSHGAEQFDEGTPKGRKQRST 187

Query: 204 FFNYFIFCLSCGALIAVTLVVWMEDNKGWQWGFGISTLTILISIPIFLLGSPTYRIKTPA 263
           FFNY++FCLSCGAL+AVT VVW+EDNKGW+WGFG+ST++I +SI +FLLGS  Y+ K P 
Sbjct: 188 FFNYYVFCLSCGALVAVTFVVWIEDNKGWEWGFGVSTISIFLSILVFLLGSRFYKNKIPR 247

Query: 264 GSPITTIFKVLASAAFNSRKAKTCNNVVMNISTSSVSSDTTTDIILDEQRQNMEKENNPI 323
           GSP+TTIFKVL +A+  S  +KT +N   +    S   + T                   
Sbjct: 248 GSPLTTIFKVLLAASIVSCSSKTSSNHFTSREVQSEHEEKT------------------- 307

Query: 324 TIPIPVSTQAMEFLNKATINNPNHPQLNCTVKQVEEAKIVLKIFPIFTSTIMLNCCLAQL 383
             P    T ++  LNKA     +H  L CTV+QVE+ KIVLK+ PIF  TIMLNCCLAQL
Sbjct: 308 --PSQSLTNSLTCLNKAIEGKTHHIWLECTVQQVEDVKIVLKMLPIFGCTIMLNCCLAQL 367

Query: 384 STFSVQQAATMNTKLGSFKVPPASLPVFPVIFIMILAPTYNHFILPIARKLTKSEMGITH 443
           ST+SV QAATMN K+ +F VP ASLPVFPV+F++ILAPTY+H I+P ARK+TKSE+GITH
Sbjct: 368 STYSVHQAATMNRKIVNFNVPSASLPVFPVVFMLILAPTYDHLIIPFARKVTKSEIGITH 427

Query: 444 LQRIGTGLVLSTVAMAVAALIETKRKTLAMKIHKLDSSEPLPITFLWVALQYLFLGSADL 503
           LQRIG GLVLS VAMAVAAL+E KRK +A +   LDS E LPITFLW+ALQYLFLGSADL
Sbjct: 428 LQRIGVGLVLSIVAMAVAALVELKRKQVAREAGLLDSEETLPITFLWIALQYLFLGSADL 487

Query: 504 FSLAGMMEFFFTEAPLSMRSLATALSWASLAMGYFLSSVLVTLVNGVTKACGFRPWLYGK 563
           F+LAG++EFFFTEAP SMRSLAT+LSWASLA+GY+LSSV+V +VN VTK+ G  PWL G+
Sbjct: 488 FTLAGLLEFFFTEAPSSMRSLATSLSWASLALGYYLSSVMVPIVNRVTKSAGQSPWL-GE 547

Query: 564 SLNRYHLERFYWVMCVLSGLNFFQYLFWANRYTYRST 599
            LNR  L+ FYW+MCVLS +NF  YLFWA RY Y ST
Sbjct: 548 KLNRNRLDLFYWLMCVLSVVNFLHYLFWAKRYKYIST 559

BLAST of Lsi06G014860 vs. TAIR 10
Match: AT3G25260.1 (Major facilitator superfamily protein )

HSP 1 Score: 382.9 bits (982), Expect = 5.0e-106
Identity = 234/576 (40.62%), Postives = 334/576 (57.99%), Query Frame = 0

Query: 20  QLNLWEDYVDWRKRPAVKGRHGGMLAASFVLVVEVLENLAFLANASNLVLYLSKFMHYSP 79
           Q+ + E + DWR + A+ G+HGG+ AA    VVE +EN+ FLA ++N ++Y +K M+YS 
Sbjct: 2   QIEMEEKFEDWRGKEAISGKHGGIKAAFIACVVETMENMVFLACSTNFMMYFTKSMNYST 61

Query: 80  SESANIVTNFMGTAFLLALLGGFLADAFFTTYSIFLISAAIESLGQAGCPVRLVKVRPAR 139
            ++A +VTNF+GT+FLL + GGF+AD+F T ++ F++  +IE LG       L+ +    
Sbjct: 62  PKAATMVTNFVGTSFLLTIFGGFVADSFLTRFAAFVLFGSIELLG-------LIMLTLQA 121

Query: 140 CVTNAAGNGNPCHKVGGGEAVMLFAGLYMVALGVGGIKGSLPPHGAEQFDETSFEGRKKR 199
            +T     G    K    ++ +LF GLY +A+GVGG+KGSLP HG    D+     ++  
Sbjct: 122 HITKLQPQGG--KKPSTPQSTVLFTGLYAIAIGVGGVKGSLPAHGG---DQIGTRNQRLI 181

Query: 200 SAFFNYFIFCLSCGALIAVTLVVWMEDNKGWQWGFGISTLTILISIPIFLLGSPTYRIKT 259
           S FFN++ F +  G  +AVTL+VW+E+N GW   F IST  +  +I +F+ G P YR K 
Sbjct: 182 SGFFNWYFFSVCLGGFLAVTLMVWIEENIGWSSSFTISTAVLASAIFVFVAGCPMYRFKR 241

Query: 260 PAGSPITTIFKVLASAAFNSRKAKTCNNVVMNISTSSVSSDTTTDIILDEQRQNMEKENN 319
           PAGSP+T I  V  SAA N  +  T   VV     S+  S                    
Sbjct: 242 PAGSPLTRIVNVFVSAARNRNRFVTDAEVVTQNHNSTDKS-------------------- 301

Query: 320 PITIPIPVSTQAMEFLNKATINNPNHPQLNCTVKQVEEAKIVLKIFPIFTSTIMLNCCLA 379
                  +     +FLNKA +NN        +  QVEE +  L + PIF STI++NCC+A
Sbjct: 302 -------IHHNKFKFLNKAKLNN------KISATQVEETRTFLALLPIFGSTIIMNCCVA 361

Query: 380 QLSTFSVQQAATMNTKLG-SFKVPPASLPVFPVIFIMILAPTYNHFILPIARKLTKSEMG 439
           Q+ TFSVQQ    N KL  SF++P ASL   P++ ++     Y  F     R L+ SE  
Sbjct: 362 QMGTFSVQQGMVTNRKLSRSFEIPVASLNAIPLLCMLSSLALYELF---GKRILSNSERS 421

Query: 440 IT-HLQRIGTGLVLSTVAMAVAALIETKRKTLAMKIHKLDSSEPLPITFLWVALQYLFLG 499
            + +L+RIG GL L++++MAVAA++E KRK  A+          + I+  W+ LQ++ L 
Sbjct: 422 SSFNLKRIGYGLALTSISMAVAAIVEVKRKHEAV-------HNNIKISVFWLELQFVMLS 481

Query: 500 SADLFSLAGMMEFFFTEAPLSMRSLATALSWASLAMGYFLSSVLVTLVNGVTKACGFRPW 559
            +D+ ++ GM+EFFF E+P SMRS++TAL W S AMG+FLSSVLV +VNG+T       W
Sbjct: 482 LSDMLTVGGMLEFFFRESPASMRSMSTALGWCSTAMGFFLSSVLVEVVNGIT------GW 515

Query: 560 LYGKSLNRYHLERFYWVMCVLSGLNFFQYLFWANRY 594
           L    LN   LE FY V+CVL+ LN F Y+FWA RY
Sbjct: 542 L-RDDLNESRLELFYLVLCVLNTLNLFNYIFWAKRY 515