Lsi06G013710 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi06G013710
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	protein MIS12 homolog
Location	chr06: 24275866 .. 24279262 (+)
RNA-Seq Expression	Lsi06G013710
Synteny	Lsi06G013710

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TGTAAGCCCGCTCCGCGTCCCCATCATTCGCCACAGCCATTTTGGCGGCAAAATTTCTCCATTTATGCCAAAGCTTTGGAGCTTCCATTTATCTCTCCATTTGAATTTCTCGTCGTCTTCTTGATTGGGAAAGAAGCTTTTTCAGGAAGCGAAAGGGAAATTTTGATGGAAGGAAGTAAAGGCGAAGCAGTATTCGATTCACTGAATCTGAATCCACAGCTCTTCATTAATGAAGCTCTCAATATTGTCGACGATTTGGTTGATGACGCTTTTGATTTCTATCAATCGTATTGCTCTCTCTATTTCCATCTCTTCAACTTAGGTTTCCAATTTTGTTCGGTTAATTTTATTGATTCTTGTCAATTCTTTTTCCCTTCAGACAAGCATCGGCCGCCTTGAAGACCGAGGGCTCCGATAGATCCCAGGATCTTACCCTGGTAAGGTTTAATTTGTATTATATTATTTGACATTTTCTTATCAGCGGTTAATTTCCGACTTTTTAAGTTTGTACTCTTAAGTAATTCATTTGAACTGTTCCGATTTTCTTTGGACAAAATTGTTTATTTGCCTCGTTCCTGTAGGGAATATCGCGGGTCCGAACCTTGGTCCAATTGGGTCTGGATAAGCGGTTGGCCATGTGGGAGAAGTACTGTCTCAATCATTGTTTTTCAGTTCCTGAAGGTTTTTCTTTACCCTCAAATGTAGGTTTTTCTTCTACTCTTTTTGTGATAAGGCTCCGGTGCCCAAGGATTAAAATATCAAAGTCGATATCAATATCGAAATTTCAAGTTATAGTTTATCAATTGAATATCAATATCGACAAATATTTGTGTAACTCAATAAATTTATAAAAAAAAGTATTTAAAATTAATAATTAAGTTGTTTTTACTTTCAAATAAGTTATAGATCTTATTGAGATTTATAGAAGACATTTTCTTATGTTTTTTACGTGTTTATAATAGATACCAGAGATGTCTACAAACCCATGTCAAATGACATATCAACAAATAATTGAAACCTTGTCTGTGCTTTGTTATGTGAAGTTGCTTTTTTAGTGTTGTTTTTGCAACTGAATTAGTGGCCCTTTACGTCCGAATCATTTCACAAGTGATTAATAATTGAGCATTTAAAATGGCTGCTTGTTGATTTTGCGAGTTTCCCACTTTTTGTGTACTGATTAGAGAGCTTCCCCAAATTTTGGTTCTGGGGCGAACAAATTTTGAAATTTTGGAGAAATGTGCATCATCAAGATTCAAGTTTTTTACAAGATATATTCATTGTATTGTTTTAGATGCAGTTTCGAGTTTCCATATTTTCTGACCTCAGATCTTCCTCATTTTGTTTGCTATAACTCAACGTGGTTCCACTGTCACTAGAAATATGGTCATGATTGTAAAAGATTCTTTGTAGCTCTAAAAAGAATGTAAGCTAACGTCTGCAGCATTTTTTAGAAAATCTCTGTCATTGGTGCATCATAGCTTTTCTTTTTCAATTATTTTTGAAGTCTAACCTATGTGAGGTCCAGTTCGACTCTTTTCATTTTGCACCAATATAGTTAGTTACTCGGGGTTGAGCAATTTCTGTCTGTTGCACTGGCATCTCAATGGAATCTGCGATATCTTATTGTCAAGTTGATAAATTATCAATGGTTGCCACATCTTGTTTGTGAACTTTGAGTTATGGTTAATTGAGTCTATGTATTCTCTTACTACTTTTTCTTCTTTCTAATGCAATAAACATATTTCTGATTCAGATCTAAATATTTTCTTTAAGTTGTCTTAGGATGAATCACCTGGTGTCACTTCAATAAGTCATGACTATGACGTGGATCTGGATACAGAGTTAGATTTTTTGAGGAATAAGCTCTCTGAGGTATTGAGTGTGTCGTATGTTAATTACTTCTTGTGTTTATGTATATTATATTTCATGAGGAAATGTATTTTTTCAGGCCAGAAAAGAGAATATTGTATTGAGCCAAGAACTACAAGCTTTGGAAAGGCAAACTGCTTCCAGTAACTCCCAAATCAATCATTTGAACGAAGCATTACAATTATATGAGCAAAATTCTGTGAATGAGATGTTCCAGGGTAATTAAGTTGTGCTTAAAATCATTTTTTTCTTCCTGCACTCCTGGGTTTACATGCGCCCTACTCTACTAGTATACTGAAGAAGGCATACTCGAAATGAAATATACAAATAATATATGAAGCTGTTTTTTCTTATAATGAACCTATATTGTCGTAGTCAGCTTGGTAGATTAAAGTTTCTGTAAACACTAACCTTCTCTCCAACTAATCGTCCTGGCCTGCATATCTCATGGTGACACAAATTCTAATAGTTCCAAATGATACTTGAACACCTGCAAAATCATTTCATTTAGGCAATTTGTATATTACCCTAAACTCTAATAGTCCCATTTTCTGCTGTTCATTCCATAAAAGAGGAACTTCAGTATGTGAATAAGGCAATTTCTCTTGCTATTTTCATTTTGAAGGCCACTTGGACAAAATTTATTCACTAGTATTAAAATTCATTGTACTGAAACTTTAGCAGTTGGCTGGTTAGTTGGGGTTTGTGCTATTTTGTGGGATATTTAGGGGGAGAGGAATAACAGAATGTTCAGAAGTGTGGAGAGGGACTATGGTGATGGTGATGTTTGGTCCTTGGTGAGGTTCTATGTTTTTCTTTGAGCTTCAGTTTTGAAGACTTTTTAATTATCCTCTGGATAGGGTTTTGCAGACTTCTTCTTTGTATTCTTTCATTTTTTTTCTCAATGAAAGTCATTTTTTCTATCAAACTTCAACGGTTGGCAGGTAAAAGCTGTTCATGATTTTCTTGCAGAAATGATAAACATTGCATCGGAGCTGCGAACGAAAATAGGAAAGCTGAAGAAAAGGAGGTTGGAGGAAACCAAGCTCACTAAAATAGAGAAGGTTCATACAAACGGAGACATTTCTCACCATCACAATGGTAGGATTTTCAAGCTACTTTATTATCAATCAAGCTGGTTTTAGCTTTCTGGAAGTTGAGTATCATCTCACATGCTGGTTGATGCTTGTTGCTGTCCTGCAGGTTTCTTTAATGCTAAGCTGGATGATATTCAAGAATTTTTAGCTGATTTGAAGACCCTTTGAAATCATTTCACTATGCCATCAACCTTACTGTATATGATGCTTGGTGTGAAACTTGTCACTATGAGTTCTTCAATGTCTTAACTTGATTTAGCTGTTGTGAGATATTTTAAACCAAATTTGTATAATATATCATTTGGATTGATGACCATCCTTAGCAATCTTCTTAGAGAGTTTGCAGATGGGCCTTCAGTAGGTGTTGGCTCAATCCTATAAATTATGATGGTTCACATTAGACTTTTGAAGCTTCTCTCATTACCTTTTCG

mRNA sequence

TGTAAGCCCGCTCCGCGTCCCCATCATTCGCCACAGCCATTTTGGCGGCAAAATTTCTCCATTTATGCCAAAGCTTTGGAGCTTCCATTTATCTCTCCATTTGAATTTCTCGTCGTCTTCTTGATTGGGAAAGAAGCTTTTTCAGGAAGCGAAAGGGAAATTTTGATGGAAGGAAGTAAAGGCGAAGCAGTATTCGATTCACTGAATCTGAATCCACAGCTCTTCATTAATGAAGCTCTCAATATTGTCGACGATTTGGTTGATGACGCTTTTGATTTCTATCAATCACAAGCATCGGCCGCCTTGAAGACCGAGGGCTCCGATAGATCCCAGGATCTTACCCTGGTAAGGGAATATCGCGGGTCCGAACCTTGGTCCAATTGGGTCTGGATAAGCGGTTGGCCATGTGGGAGAAGTACTGTCTCAATCATTGTTTTTCAGTTCCTGAAGGATGAATCACCTGGTGTCACTTCAATAAGTCATGACTATGACGTGGATCTGGATACAGAGTTAGATTTTTTGAGGAATAAGCTCTCTGAGGCCAGAAAAGAGAATATTGTATTGAGCCAAGAACTACAAGCTTTGGAAAGGCAAACTGCTTCCAGTAACTCCCAAATCAATCATTTGAACGAAGCATTACAATTATATGAGCAAAATTCTGTGAATGAGATGTTCCAGGGTTTCTTTAATGCTAAGCTGGATGATATTCAAGAATTTTTAGCTGATTTGAAGACCCTTTGAAATCATTTCACTATGCCATCAACCTTACTGTATATGATGCTTGGTGTGAAACTTGTCACTATGAGTTCTTCAATGTCTTAACTTGATTTAGCTGTTGTGAGATATTTTAAACCAAATTTGTATAATATATCATTTGGATTGATGACCATCCTTAGCAATCTTCTTAGAGAGTTTGCAGATGGGCCTTCAGTAGGTGTTGGCTCAATCCTATAAATTATGATGGTTCACATTAGACTTTTGAAGCTTCTCTCATTACCTTTTCG

Coding sequence (CDS)

ATGGAAGGAAGTAAAGGCGAAGCAGTATTCGATTCACTGAATCTGAATCCACAGCTCTTCATTAATGAAGCTCTCAATATTGTCGACGATTTGGTTGATGACGCTTTTGATTTCTATCAATCACAAGCATCGGCCGCCTTGAAGACCGAGGGCTCCGATAGATCCCAGGATCTTACCCTGGTAAGGGAATATCGCGGGTCCGAACCTTGGTCCAATTGGGTCTGGATAAGCGGTTGGCCATGTGGGAGAAGTACTGTCTCAATCATTGTTTTTCAGTTCCTGAAGGATGAATCACCTGGTGTCACTTCAATAAGTCATGACTATGACGTGGATCTGGATACAGAGTTAGATTTTTTGAGGAATAAGCTCTCTGAGGCCAGAAAAGAGAATATTGTATTGAGCCAAGAACTACAAGCTTTGGAAAGGCAAACTGCTTCCAGTAACTCCCAAATCAATCATTTGAACGAAGCATTACAATTATATGAGCAAAATTCTGTGAATGAGATGTTCCAGGGTTTCTTTAATGCTAAGCTGGATGATATTCAAGAATTTTTAGCTGATTTGAAGACCCTTTGA

Protein sequence

MEGSKGEAVFDSLNLNPQLFINEALNIVDDLVDDAFDFYQSQASAALKTEGSDRSQDLTLVREYRGSEPWSNWVWISGWPCGRSTVSIIVFQFLKDESPGVTSISHDYDVDLDTELDFLRNKLSEARKENIVLSQELQALERQTASSNSQINHLNEALQLYEQNSVNEMFQGFFNAKLDDIQEFLADLKTL

Homology

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BLAST of Lsi06G013710 vs. ExPASy Swiss-Prot
Match: Q2V0Z5 (Protein MIS12 homolog OS=Arabidopsis thaliana OX=3702 GN=MIS12 PE=3 SV=1)

HSP 1 Score: 124.0 bits (310), Expect = 1.8e-27
Identity = 78/182 (42.86%), Postives = 109/182 (59.89%), Query Frame = 0


Query: 1   MEGSKGEAVFDSLNLNPQLFINEALNIVDDLVDDAFDFYQSQASAALKTEGSDRSQDLTL 60
           MEGSK EAVFDS+NLNPQ+FINEA+N V+D VD AFDFY   AS +LK +GSD+ +   L
Sbjct: 1   MEGSKSEAVFDSMNLNPQIFINEAINSVEDYVDQAFDFYARDASKSLKIKGSDKQKSQAL 60

Query: 61  ---VREYRG------SEPWSNWVWISGWPCGRSTVSIIVFQFLKDESPGVTSISHD--YD 120
              +   RG            W   S   C       +   F+  +S   +S+  D  YD
Sbjct: 61  SNGIARVRGLLLSVIDNRLKLWESYSLRFC-----FAVPDGFVLPKSEESSSVHQDGLYD 120

Query: 121 VDLDTELDFLRNKLSEARKENIVLSQELQALERQTASSNSQINHLNEALQLYEQNSVNEM 172
           ++LD ELD LR+KL+   K ++ L  ELQALER + S    +  +NEAL+LY+++S++E+
Sbjct: 121 LELDAELDSLRDKLNVVGKRSVELDSELQALERSSVSRERSLRLVNEALELYDESSMDEI 177

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BLAST of Lsi06G013710 vs. ExPASy TrEMBL
Match: A0A5A7US57 (Centromere protein Mis12 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold629G00040 PE=3 SV=1)

HSP 1 Score: 235.7 bits (600), Expect = 1.6e-58
Identity = 145/239 (60.67%), Postives = 159/239 (66.53%), Query Frame = 0


Query: 1   MEGSKGEAVFDSLNLNPQLFINEALNIVDDLVDDAFDFYQSQASAALKTEGSDRSQDLTL 60
           MEGSKGE+VF+SLNLNPQLFINEALN VDDLVDDAFDFYQSQASAALKTEGSDRSQDLTL
Sbjct: 1   MEGSKGESVFNSLNLNPQLFINEALNTVDDLVDDAFDFYQSQASAALKTEGSDRSQDLTL 60

Query: 61  -VREYRGSEPWSNWVWISGWP--CGRSTVSIIVFQFL--KDESPGVTSISHDYDVDLDTE 120
            + + R          ++ W   C     S+     L   DESPGVTSI+HD DVDLDTE
Sbjct: 61  GISQVRALVQLGLDKRLAMWEKYCLNHCFSVPEGFSLPSNDESPGVTSINHDQDVDLDTE 120

Query: 121 LDFLRNKLSEARKENIVLSQELQALERQTASSNSQINHLNEALQLYEQNSVNEMFQ---- 180
           LD LRNKLSE RKENIVL+QELQALERQTASSNSQI+H NEALQLYEQ+SVN+MFQ    
Sbjct: 121 LDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQEMIK 180

Query: 181 ---------------------------------------GFFNAKLDDIQEFLADLKTL 192
                                                  GF NAKLDDIQEFL+DLK +
Sbjct: 181 TASELRAKIGKMKKRRTEEAKLAKVEKVHTNGDISHHHNGFSNAKLDDIQEFLSDLKKI 239

[Close]

BLAST of Lsi06G013710 vs. ExPASy TrEMBL
Match: A0A5A7UQ95 (Centromere protein Mis12 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold274G003370 PE=3 SV=1)

HSP 1 Score: 234.2 bits (596), Expect = 4.6e-58
Identity = 144/239 (60.25%), Postives = 158/239 (66.11%), Query Frame = 0


Query: 1   MEGSKGEAVFDSLNLNPQLFINEALNIVDDLVDDAFDFYQSQASAALKTEGSDRSQDLTL 60
           MEGSKGE+VF+SLNLNPQLFINEALN VDDLVDDAFDFYQSQASA LKTEGSDRSQDLTL
Sbjct: 1   MEGSKGESVFNSLNLNPQLFINEALNTVDDLVDDAFDFYQSQASATLKTEGSDRSQDLTL 60

Query: 61  -VREYRGSEPWSNWVWISGWP--CGRSTVSIIVFQFL--KDESPGVTSISHDYDVDLDTE 120
            + + R          ++ W   C     S+     L   DESPGVTSI+HD DVDLDTE
Sbjct: 61  GISQVRALVQLGLDKRLAMWEKYCLNHCFSVPEGFSLPSNDESPGVTSINHDQDVDLDTE 120

Query: 121 LDFLRNKLSEARKENIVLSQELQALERQTASSNSQINHLNEALQLYEQNSVNEMFQ---- 180
           LD LRNKLSE RKENIVL+QELQALERQTASSNSQI+H NEALQLYEQ+SVN+MFQ    
Sbjct: 121 LDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQEMIK 180

Query: 181 ---------------------------------------GFFNAKLDDIQEFLADLKTL 192
                                                  GF NAKLDDIQEFL+DLK +
Sbjct: 181 TASELRSKIGKMKKRRTEEAKLAKVEKVHTNGDISHHHNGFSNAKLDDIQEFLSDLKKI 239

[Close]

BLAST of Lsi06G013710 vs. ExPASy TrEMBL
Match: A0A0A0LPL2 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G014450 PE=3 SV=1)

HSP 1 Score: 233.4 bits (594), Expect = 7.8e-58
Identity = 144/238 (60.50%), Postives = 157/238 (65.97%), Query Frame = 0


Query: 1   MEGSKGEAVFDSLNLNPQLFINEALNIVDDLVDDAFDFYQSQASAALKTEGSDRSQDLTL 60
           MEGSKGE VF+SLNLNPQLFINEALN VDDLVDDAFDFYQSQASAALKTE SDRSQDLTL
Sbjct: 1   MEGSKGEVVFNSLNLNPQLFINEALNTVDDLVDDAFDFYQSQASAALKTESSDRSQDLTL 60

Query: 61  -VREYRGSEPWSNWVWISGWP--CGRSTVSIIVFQFL--KDESPGVTSISHDYDVDLDTE 120
            + + R          ++ W   C     S+     L   DESPGVTSISHD+DVDLDTE
Sbjct: 61  GISQVRALVQLGLHKRLAMWEKYCLNHCFSVPEGFSLPSDDESPGVTSISHDHDVDLDTE 120

Query: 121 LDFLRNKLSEARKENIVLSQELQALERQTASSNSQINHLNEALQLYEQNSVNEMFQ---- 180
           LD LRNKLSE RKENIVL+QELQALERQTASSNSQI+H NEALQLYEQ+SVN+MFQ    
Sbjct: 121 LDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQEMIR 180

Query: 181 --------------------------------------GFFNAKLDDIQEFLADLKTL 192
                                                 GF NAKLDDIQEFL+ LK +
Sbjct: 181 TASELRVKIGKLKKRTEETKLAIVEKVHTNGDISHHHKGFSNAKLDDIQEFLSGLKKI 238

[Close]

BLAST of Lsi06G013710 vs. ExPASy TrEMBL
Match: A0A6J1JME3 (protein MIS12 homolog isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111485708 PE=3 SV=1)

HSP 1 Score: 229.2 bits (583), Expect = 1.5e-56
Identity = 149/248 (60.08%), Postives = 156/248 (62.90%), Query Frame = 0


Query: 1   MEGSKGEAVFDSLNLNPQLFINEALNIVDDLVDDAFDFYQSQASAALKTEGSDRSQDLTL 60
           MEGSKGE VFDSLNLNPQLFINEALNIVDDLVDDAFDFYQ+QASA+LKTEGSDRSQDL L
Sbjct: 1   MEGSKGEEVFDSLNLNPQLFINEALNIVDDLVDDAFDFYQTQASASLKTEGSDRSQDLIL 60

Query: 61  ----VREYRGS------EPWSNWV--WISGWPCGRSTVSIIVFQFLKDESPGVTSISHD- 120
               VR +  S        W  +        P G S  S        DESP VTS+S D 
Sbjct: 61  GISRVRTFVQSGLDKRLAMWEKYCLNHCFSVPEGFSLPS-------NDESPVVTSVSRDC 120

Query: 121 -YDVDLDTELDFLRNKLSEARKENIVLSQELQALERQTASSNSQINHLNEALQLYEQNSV 180
            YDVDLDTELDFLR  LSE RKENIVL+QELQALERQTASSNSQ+N  NEALQLYEQNSV
Sbjct: 121 LYDVDLDTELDFLRKNLSEVRKENIVLNQELQALERQTASSNSQVNRFNEALQLYEQNSV 180

Query: 181 NEMFQ-------------------------------------------GFFNAKLDDIQE 192
           NEMFQ                                           GF NAKLDDIQE
Sbjct: 181 NEMFQEMMGTASELRAKIGKLKKRRMEESKLTKVEKIHTNRDISHHHKGFSNAKLDDIQE 240

[Close]

BLAST of Lsi06G013710 vs. ExPASy TrEMBL
Match: A0A6J1JD43 (protein MIS12 homolog isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111485708 PE=3 SV=1)

HSP 1 Score: 228.8 bits (582), Expect = 1.9e-56
Identity = 149/249 (59.84%), Postives = 156/249 (62.65%), Query Frame = 0


Query: 1   MEGSKGEAVFDSLNLNPQLFINEALNIVDDLVDDAFDFYQSQASAALKTEGSDRSQDLTL 60
           MEGSKGE VFDSLNLNPQLFINEALNIVDDLVDDAFDFYQ+QASA+LKTEGSDRSQDL L
Sbjct: 1   MEGSKGEEVFDSLNLNPQLFINEALNIVDDLVDDAFDFYQTQASASLKTEGSDRSQDLIL 60

Query: 61  ----VREYRGS------EPWSNWV--WISGWPCGRSTVSIIVFQFLKDESPGVTSISHD- 120
               VR +  S        W  +        P G S  S        DESP VTS+S D 
Sbjct: 61  GISRVRTFVQSGLDKRLAMWEKYCLNHCFSVPEGFSLPS-------NDESPVVTSVSRDC 120

Query: 121 -YDVDLDTELDFLRNKLSEARKENIVLSQELQALERQTASSNSQINHLNEALQLYEQNSV 180
            YDVDLDTELDFLR  LSE RKENIVL+QELQALERQTASSNSQ+N  NEALQLYEQNSV
Sbjct: 121 LYDVDLDTELDFLRKNLSEVRKENIVLNQELQALERQTASSNSQVNRFNEALQLYEQNSV 180

Query: 181 NEMFQ--------------------------------------------GFFNAKLDDIQ 192
           NEMFQ                                            GF NAKLDDIQ
Sbjct: 181 NEMFQAEMMGTASELRAKIGKLKKRRMEESKLTKVEKIHTNRDISHHHKGFSNAKLDDIQ 240

[Close]

BLAST of Lsi06G013710 vs. NCBI nr
Match: XP_031742242.1 (protein MIS12 homolog isoform X2 [Cucumis sativus] >KGN63753.2 hypothetical protein Csa_013161 [Cucumis sativus])

HSP 1 Score: 304.3 bits (778), Expect = 7.4e-79
Identity = 166/233 (71.24%), Postives = 175/233 (75.11%), Query Frame = 0


Query: 1   MEGSKGEAVFDSLNLNPQLFINEALNIVDDLVDDAFDFYQSQASAALKTEGSDRSQDLTL 60
           MEGSKGE VF+SLNLNPQLFINEALN VDDLVDDAFDFYQSQASAALKTE SDRSQDLTL
Sbjct: 1   MEGSKGEVVFNSLNLNPQLFINEALNTVDDLVDDAFDFYQSQASAALKTESSDRSQDLTL 60

Query: 61  VREYRGSEPWSNWVWISGWPCGRSTVSIIVFQFLKDESPGVTSISHDYDVDLDTELDFLR 120
           VREYR SE WSNWV IS WPCG+ST  I VFQ LKDESPGVTSISHD+DVDLDTELD LR
Sbjct: 61  VREYRKSERWSNWVCISDWPCGKSTALITVFQCLKDESPGVTSISHDHDVDLDTELDLLR 120

Query: 121 NKLSEARKENIVLSQELQALERQTASSNSQINHLNEALQLYEQNSVNEMFQ--------- 180
           NKLSE RKENIVL+QELQALERQTASSNSQI+H NEALQLYEQ+SVN+MFQ         
Sbjct: 121 NKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQEMIRTASEL 180

Query: 181 ---------------------------------GFFNAKLDDIQEFLADLKTL 192
                                            GF NAKLDDIQEFL+DLK +
Sbjct: 181 RVKIGKLKKRTEETKLAIVEKVHTNGDISHHHKGFSNAKLDDIQEFLSDLKKI 233

[Close]

BLAST of Lsi06G013710 vs. NCBI nr
Match: XP_038879945.1 (protein MIS12 homolog [Benincasa hispida])

HSP 1 Score: 237.3 bits (604), Expect = 1.1e-58
Identity = 148/239 (61.92%), Postives = 156/239 (65.27%), Query Frame = 0


Query: 1   MEGSKGEAVFDSLNLNPQLFINEALNIVDDLVDDAFDFYQSQASAALKTEGSDRSQDLTL 60
           MEGSKGEAVFDSLNLNPQLFINEALNIVDDLVDDAF FYQSQASA LKTEGSDRSQDLTL
Sbjct: 31  MEGSKGEAVFDSLNLNPQLFINEALNIVDDLVDDAFGFYQSQASATLKTEGSDRSQDLTL 90

Query: 61  -VREYRGSEPWSNWVWISGWP--CGRSTVSIIVFQFL--KDESPGVTSISHDYDVDLDTE 120
            +   R          ++ W   C     S+     L   D+SPGVTSISHDYDV LDTE
Sbjct: 91  GISRVRALVQLGLDKRLAMWEKYCLNHCFSVPEGFSLPSNDDSPGVTSISHDYDVGLDTE 150

Query: 121 LDFLRNKLSEARKENIVLSQELQALERQTASSNSQINHLNEALQLYEQNSVNEMFQ---- 180
           LDFLRNKLSE RKENIVL+QELQALERQTA+SNSQINH NEALQLYEQNSVNEMFQ    
Sbjct: 151 LDFLRNKLSEVRKENIVLNQELQALERQTATSNSQINHFNEALQLYEQNSVNEMFQEMMG 210

Query: 181 ---------------------------------------GFFNAKLDDIQEFLADLKTL 192
                                                  G  NAKLDDI+ FL DLKTL
Sbjct: 211 TASELRAKIGKLKKRRMEESKLTKVEKVHTNGDISHYHNGLSNAKLDDIKIFLDDLKTL 269

[Close]

BLAST of Lsi06G013710 vs. NCBI nr
Match: XP_004138204.3 (protein MIS12 homolog isoform X1 [Cucumis sativus])

HSP 1 Score: 236.1 bits (601), Expect = 2.5e-58
Identity = 145/238 (60.92%), Postives = 158/238 (66.39%), Query Frame = 0


Query: 1   MEGSKGEAVFDSLNLNPQLFINEALNIVDDLVDDAFDFYQSQASAALKTEGSDRSQDLTL 60
           MEGSKGE VF+SLNLNPQLFINEALN VDDLVDDAFDFYQSQASAALKTE SDRSQDLTL
Sbjct: 1   MEGSKGEVVFNSLNLNPQLFINEALNTVDDLVDDAFDFYQSQASAALKTESSDRSQDLTL 60

Query: 61  -VREYRGSEPWSNWVWISGWP--CGRSTVSIIVFQFL--KDESPGVTSISHDYDVDLDTE 120
            + + R          ++ W   C     S+     L   DESPGVTSISHD+DVDLDTE
Sbjct: 61  GISQVRALVQLGLHKRLAMWEKYCLNHCFSVPEGFSLPSDDESPGVTSISHDHDVDLDTE 120

Query: 121 LDFLRNKLSEARKENIVLSQELQALERQTASSNSQINHLNEALQLYEQNSVNEMFQ---- 180
           LD LRNKLSE RKENIVL+QELQALERQTASSNSQI+H NEALQLYEQ+SVN+MFQ    
Sbjct: 121 LDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQEMIR 180

Query: 181 --------------------------------------GFFNAKLDDIQEFLADLKTL 192
                                                 GF NAKLDDIQEFL+DLK +
Sbjct: 181 TASELRVKIGKLKKRTEETKLAIVEKVHTNGDISHHHKGFSNAKLDDIQEFLSDLKKI 238

[Close]

BLAST of Lsi06G013710 vs. NCBI nr
Match: KAA0058004.1 (Centromere protein Mis12 [Cucumis melo var. makuwa] >TYK28378.1 Centromere protein Mis12 [Cucumis melo var. makuwa])

HSP 1 Score: 235.7 bits (600), Expect = 3.2e-58
Identity = 145/239 (60.67%), Postives = 159/239 (66.53%), Query Frame = 0


Query: 1   MEGSKGEAVFDSLNLNPQLFINEALNIVDDLVDDAFDFYQSQASAALKTEGSDRSQDLTL 60
           MEGSKGE+VF+SLNLNPQLFINEALN VDDLVDDAFDFYQSQASAALKTEGSDRSQDLTL
Sbjct: 1   MEGSKGESVFNSLNLNPQLFINEALNTVDDLVDDAFDFYQSQASAALKTEGSDRSQDLTL 60

Query: 61  -VREYRGSEPWSNWVWISGWP--CGRSTVSIIVFQFL--KDESPGVTSISHDYDVDLDTE 120
            + + R          ++ W   C     S+     L   DESPGVTSI+HD DVDLDTE
Sbjct: 61  GISQVRALVQLGLDKRLAMWEKYCLNHCFSVPEGFSLPSNDESPGVTSINHDQDVDLDTE 120

Query: 121 LDFLRNKLSEARKENIVLSQELQALERQTASSNSQINHLNEALQLYEQNSVNEMFQ---- 180
           LD LRNKLSE RKENIVL+QELQALERQTASSNSQI+H NEALQLYEQ+SVN+MFQ    
Sbjct: 121 LDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQEMIK 180

Query: 181 ---------------------------------------GFFNAKLDDIQEFLADLKTL 192
                                                  GF NAKLDDIQEFL+DLK +
Sbjct: 181 TASELRAKIGKMKKRRTEEAKLAKVEKVHTNGDISHHHNGFSNAKLDDIQEFLSDLKKI 239

[Close]

BLAST of Lsi06G013710 vs. NCBI nr
Match: KAA0058032.1 (Centromere protein Mis12 [Cucumis melo var. makuwa])

HSP 1 Score: 234.2 bits (596), Expect = 9.4e-58
Identity = 144/239 (60.25%), Postives = 158/239 (66.11%), Query Frame = 0


Query: 1   MEGSKGEAVFDSLNLNPQLFINEALNIVDDLVDDAFDFYQSQASAALKTEGSDRSQDLTL 60
           MEGSKGE+VF+SLNLNPQLFINEALN VDDLVDDAFDFYQSQASA LKTEGSDRSQDLTL
Sbjct: 1   MEGSKGESVFNSLNLNPQLFINEALNTVDDLVDDAFDFYQSQASATLKTEGSDRSQDLTL 60

Query: 61  -VREYRGSEPWSNWVWISGWP--CGRSTVSIIVFQFL--KDESPGVTSISHDYDVDLDTE 120
            + + R          ++ W   C     S+     L   DESPGVTSI+HD DVDLDTE
Sbjct: 61  GISQVRALVQLGLDKRLAMWEKYCLNHCFSVPEGFSLPSNDESPGVTSINHDQDVDLDTE 120

Query: 121 LDFLRNKLSEARKENIVLSQELQALERQTASSNSQINHLNEALQLYEQNSVNEMFQ---- 180
           LD LRNKLSE RKENIVL+QELQALERQTASSNSQI+H NEALQLYEQ+SVN+MFQ    
Sbjct: 121 LDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQEMIK 180

Query: 181 ---------------------------------------GFFNAKLDDIQEFLADLKTL 192
                                                  GF NAKLDDIQEFL+DLK +
Sbjct: 181 TASELRSKIGKMKKRRTEEAKLAKVEKVHTNGDISHHHNGFSNAKLDDIQEFLSDLKKI 239

[Close]

BLAST of Lsi06G013710 vs. TAIR 10
Match: AT5G35520.1 (minichromosome instability 12 (mis12)-like )

HSP 1 Score: 124.0 bits (310), Expect = 1.3e-28
Identity = 78/182 (42.86%), Postives = 109/182 (59.89%), Query Frame = 0


Query: 1   MEGSKGEAVFDSLNLNPQLFINEALNIVDDLVDDAFDFYQSQASAALKTEGSDRSQDLTL 60
           MEGSK EAVFDS+NLNPQ+FINEA+N V+D VD AFDFY   AS +LK +GSD+ +   L
Sbjct: 1   MEGSKSEAVFDSMNLNPQIFINEAINSVEDYVDQAFDFYARDASKSLKIKGSDKQKSQAL 60

Query: 61  ---VREYRG------SEPWSNWVWISGWPCGRSTVSIIVFQFLKDESPGVTSISHD--YD 120
              +   RG            W   S   C       +   F+  +S   +S+  D  YD
Sbjct: 61  SNGIARVRGLLLSVIDNRLKLWESYSLRFC-----FAVPDGFVLPKSEESSSVHQDGLYD 120

Query: 121 VDLDTELDFLRNKLSEARKENIVLSQELQALERQTASSNSQINHLNEALQLYEQNSVNEM 172
           ++LD ELD LR+KL+   K ++ L  ELQALER + S    +  +NEAL+LY+++S++E+
Sbjct: 121 LELDAELDSLRDKLNVVGKRSVELDSELQALERSSVSRERSLRLVNEALELYDESSMDEI 177

The following BLAST results are available for this feature:

BLAST of Lsi06G013710 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q2V0Z5	1.8e-27	42.86	Protein MIS12 homolog OS=Arabidopsis thaliana OX=3702 GN=MIS12 PE=3 SV=1	[more]

BLAST of Lsi06G013710 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7US57	1.6e-58	60.67	Centromere protein Mis12 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffol...	[more]
A0A5A7UQ95	4.6e-58	60.25	Centromere protein Mis12 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffol...	[more]
A0A0A0LPL2	7.8e-58	60.50	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G014450 PE=3 SV=1	[more]
A0A6J1JME3	1.5e-56	60.08	protein MIS12 homolog isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111485708 PE=...	[more]
A0A6J1JD43	1.9e-56	59.84	protein MIS12 homolog isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111485708 PE=...	[more]

BLAST of Lsi06G013710 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_031742242.1	7.4e-79	71.24	protein MIS12 homolog isoform X2 [Cucumis sativus] >KGN63753.2 hypothetical prot...	[more]
XP_038879945.1	1.1e-58	61.92	protein MIS12 homolog [Benincasa hispida]	[more]
XP_004138204.3	2.5e-58	60.92	protein MIS12 homolog isoform X1 [Cucumis sativus]	[more]
KAA0058004.1	3.2e-58	60.67	Centromere protein Mis12 [Cucumis melo var. makuwa] >TYK28378.1 Centromere prote...	[more]
KAA0058032.1	9.4e-58	60.25	Centromere protein Mis12 [Cucumis melo var. makuwa]	[more]

BLAST of Lsi06G013710 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G35520.1	1.3e-28	42.86	minichromosome instability 12 (mis12)-like	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18

Position :

Zoom :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 109..157
IPR008685	Centromere protein Mis12	PANTHER	PTHR14527	PROTEIN MIS12 HOMOLOG	coord: 1..58 coord: 107..173

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi06G013710.1	Lsi06G013710.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0051276	chromosome organization
biological_process	GO:0000278	mitotic cell cycle
cellular_component	GO:0000776	kinetochore
cellular_component	GO:0005634	nucleus
cellular_component	GO:0000775	chromosome, centromeric region