Lsi06G012460 (gene) Bottle gourd (USVL1VR-Ls) v1

BLAST of Lsi06G012460 vs. ExPASy Swiss-Prot
Match: P21885 (Arginine decarboxylase OS=Bacillus subtilis (strain 168) OX=224308 GN=speA PE=1 SV=2)

HSP 1 Score: 327.4 bits (838), Expect = 3.3e-88
Identity = 179/478 (37.45%), Postives = 269/478 (56.28%), Query Frame = 0

Query: 113 PLVDALKVSSEQNAARFHFPGHNRGRAGPPSFTQLIGLKAFMHDLPELPELDNLFCPEGP 172
           PL   LK  + +   +FH PGH +G    P F Q IG  A   DL  +  LD+L  P+G 
Sbjct: 7   PLYTGLKKHASRQPVQFHIPGHKKGAGMDPEFRQFIGENALSIDLINIEPLDDLHAPKGI 66

Query: 173 ILEAQQQAAKLFGASETWFLVGGTTCGIQAAIMATCSPGDHIILPRNSHVSVISALVLSG 232
           I +AQ  AA+ FGA  T+F V GT+  I   +MA C PGD II+PRN H S+++A+V SG
Sbjct: 67  IKQAQDLAAEAFGADHTFFSVQGTSGAIMTMVMAVCGPGDKIIIPRNVHKSIMTAIVFSG 126

Query: 233 AIPKYIMPVYDSNWDIA-GEVDRAIKDLEMEGQKASAVFVTSPTYHGICSNLGEISQICH 292
           A+P +I P  D+   I+ G    + K    E   A  + V +PTY G+ ++L  I ++ H
Sbjct: 127 AVPIFIHPEIDNELGISHGITLESAKRALTEHPDAKGLLVINPTYFGVAADLKSIVELAH 186

Query: 293 VKGIPFIVDEAHGAHFGFQPQLPLSALQQGVDLAVQSTHKVLYSLTQSSMLHMSGNIVDK 352
              +P +VDEAHG H  F  +LPLSA+Q G D+A  S HK+  SLTQSS+L+M   +V K
Sbjct: 187 SFDVPVLVDEAHGVHIHFHDELPLSAMQAGADIAATSVHKLGGSLTQSSILNMREGLVSK 246

Query: 353 ERICRCLQTLQSTSPSYLLLASLDAARAQLSDNPDKIFNRAIYLAYQAKRKINKISGVSI 412
           +R+   L  L +TS SYLLLASLD AR +L+    ++    + LA Q + ++N+I G+  
Sbjct: 247 DRVQSILSMLTTTSTSYLLLASLDVARKRLATEGRQLAEETLKLANQTRDRLNQIEGIYC 306

Query: 413 LEFPMFSNFPA--IDPLRLTIGFQQLGLSGYEADEILYKNHNIVCELVGNQSITFIINLG 472
           +   +  +  A   DP +L I  + LGL+G++ ++ L ++ NI  EL    +I  I   G
Sbjct: 307 VGSEILGSKAAYSYDPTKLIISVKSLGLTGHDVEKWLRESFNIEVELSDLYNILCIFTPG 366

Query: 473 TSEDDIEILVSGIEDVSSFASIMRIEGRTKPIVSAPFPNVKISLNPRDAFFAKKRRENIK 532
            S++D + LV  + +++   S   +  +   ++    P   +++ PRDAF+A      +K
Sbjct: 367 DSQNDADRLVEALTEIAQQMSEQDVTHQQTEVLLPEIP--LLAMTPRDAFYANTEVIPLK 426

Query: 533 ECVGKVCGELICLYPPGIPVMIPGEIISEEVVDYLLHLKGKGASISGASDPKLSSLLV 588
           E  G++  E + +YPPGIP+ IPGEII+EE + Y+      G  + G  D  L  + V
Sbjct: 427 EASGRIIAEFVMVYPPGIPIFIPGEIITEENISYIFKNLDAGLPVQGPEDSTLHMIRV 482

BLAST of Lsi06G012460 vs. ExPASy Swiss-Prot
Match: Q9K9K5 (Arginine decarboxylase OS=Bacillus halodurans (strain ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125) OX=272558 GN=speA PE=3 SV=1)

HSP 1 Score: 318.9 bits (816), Expect = 1.2e-85
Identity = 182/479 (38.00%), Postives = 270/479 (56.37%), Query Frame = 0

Query: 113 PLVDALKVSSEQNAARFHFPGHNRGRAGPPSFTQLIGLKAFMHDLPELPELDNLFCPEGP 172
           PL   +   ++ N  +FH PGH +G    P+F   IG  A   DL  +  LD+L  P G 
Sbjct: 8   PLFSGVVAHAKHNPVQFHIPGHKKGAGMDPAFRSFIGDNALSIDLINIGPLDDLHHPHGI 67

Query: 173 ILEAQQQAAKLFGASETWFLVGGTTCGIQAAIMATCSPGDHIILPRNSHVSVISALVLSG 232
           I EAQ+ AA+ FGA  T+F V GT+  I   IM+   PG+ II+PRN H S++SA+V SG
Sbjct: 68  IKEAQELAAEAFGADHTFFSVQGTSGAIMTMIMSVVGPGEKIIVPRNVHKSIMSAIVFSG 127

Query: 233 AIPKYIMPVYDSNWDIA-GEVDRAIKDLEMEGQKASAVFVTSPTYHGICSNLGEISQICH 292
           A P +I P  D    I+ G    A++        A  + V +PTY GI +NL +I ++CH
Sbjct: 128 ATPVFIHPEIDPLLGISHGITIEAVEKALDAHPDAKGLLVINPTYFGIAANLKKIVELCH 187

Query: 293 VKGIPFIVDEAHGAHFGFQPQLPLSALQQGVDLAVQSTHKVLYSLTQSSMLHMSGNIVDK 352
            + +P +VDEAHG H  F   LPLSA+Q G D+A  S HK+  SLTQSS+L++   +V  
Sbjct: 188 SRDVPVLVDEAHGVHIHFHEALPLSAMQAGADMAATSVHKLGGSLTQSSILNVREGLVSA 247

Query: 353 ERICRCLQTLQSTSPSYLLLASLDAARAQLSDNPDKIFNRAIYLAYQAKRKINKISGVSI 412
           +R+   +  L +TS SYLLLASLDAAR  L+ N   +    I LA QA+ +IN I G+  
Sbjct: 248 KRVQTIISMLTTTSTSYLLLASLDAARKHLATNGRDLIGYTIQLADQARDQINAIDGLYC 307

Query: 413 LEFPMFSNFPA--IDPLRLTIGFQQLGLSGYEADEILYKNHNIVCELVGNQSITFIINLG 472
           +   +         DP +L I  + LG++GY+A+  L +++ I  EL    +I  I++ G
Sbjct: 308 VGKEILGTIATYDYDPTKLIISVKNLGITGYDAEVWLREHYQIEVELSDLYNILCIVSFG 367

Query: 473 TSEDDIEILVSGIEDVSSFASIMRIEGRTKPIVSAPFPNV-KISLNPRDAFFAKKRRENI 532
            +E ++++LV  +   S  A + +     +  VS   PN+  ++++PRDAF+A+      
Sbjct: 368 DTEREMDLLVKAL---SELADLHKHGICERSPVSVYVPNIPTLAMSPRDAFYAETEVVPF 427

Query: 533 KECVGKVCGELICLYPPGIPVMIPGEIISEEVVDYLLHLKGKGASISGASDPKLSSLLV 588
           ++ VG+   E I +YPPGIP++IPGEII+E  + Y+      G  + G  D    +L V
Sbjct: 428 EDSVGRTIAEFIMVYPPGIPILIPGEIITESNLAYIRENNRAGLPVQGPEDDTFRTLRV 483

BLAST of Lsi06G012460 vs. ExPASy Swiss-Prot
Match: Q81MS2 (Arginine decarboxylase OS=Bacillus anthracis OX=1392 GN=speA PE=3 SV=1)

HSP 1 Score: 308.5 bits (789), Expect = 1.6e-82
Identity = 169/482 (35.06%), Postives = 270/482 (56.02%), Query Frame = 0

Query: 113 PLVDALKVSSEQNAARFHFPGHNRGRAGPPSFTQLIGLKAFMHDLPELPELDNLFCPEGP 172
           PL  AL   S++N  +FH PGH +G+   P F + IG  A   DL  +  LD+L  P+G 
Sbjct: 10  PLFTALVEHSKRNPIQFHIPGHKKGQGMDPEFREFIGHNALAIDLINIAPLDDLHHPKGM 69

Query: 173 ILEAQQQAAKLFGASETWFLVGGTTCGIQAAIMATCSPGDHIILPRNSHVSVISALVLSG 232
           I EAQ  AA  FGA  T+F + GT+  I   +M+ C PGD I++PRN H SV+SA++ SG
Sbjct: 70  IKEAQDLAAAAFGADHTFFSIQGTSGAIMTMVMSVCGPGDKILVPRNVHKSVMSAIIFSG 129

Query: 233 AIPKYIMPVYDSNWDIA-GEVDRAIKDLEMEGQKASAVFVTSPTYHGICSNLGEISQICH 292
           A P ++ P  D    I+ G   +++K    E   A  + V +PTY G  ++L +I Q+ H
Sbjct: 130 AKPIFMHPEIDPKLGISHGITIQSVKKALEEHSDAKGLLVINPTYFGFAADLEQIVQLAH 189

Query: 293 VKGIPFIVDEAHGAHFGFQPQLPLSALQQGVDLAVQSTHKVLYSLTQSSMLHMSGNIVDK 352
              IP +VDEAHG H  F  +LP+SA+Q G D+A  S HK+  SLTQSS+L++   +V+ 
Sbjct: 190 SYDIPVLVDEAHGVHIHFHDELPMSAMQAGADMAATSVHKLGGSLTQSSILNVKEGLVNV 249

Query: 353 ERICRCLQTLQSTSPSYLLLASLDAARAQLSDNPDKIFNRAIYLAYQAKRKINKIS---- 412
           + +   +  L +TS SY+LLASLD AR +L+     +  + I LA Q +  IN I     
Sbjct: 250 KHVQSIISMLTTTSTSYILLASLDVARKRLATEGKALIEQTIQLAEQVRNAINDIEHLYC 309

Query: 413 -GVSILEFPMFSNFPAIDPLRLTIGFQQLGLSGYEADEILYKNHNIVCELVGNQSITFII 472
            G  +L      N+   DP ++ +  + LG++G++A+  L + +NI  EL    +I  ++
Sbjct: 310 PGKEMLGTDATFNY---DPTKIIVSVKDLGITGHQAEVWLREQYNIEVELSDLYNILCLV 369

Query: 473 NLGTSEDDIEILVSGIEDVSSFASIMRIEGRTKPIVSAPFPNVKI-SLNPRDAFFAKKRR 532
             G +E +   L++ ++D+S   +I + +      +    P + + +L+PRDAF+++   
Sbjct: 370 TFGDTESETNTLIAALQDLS---AIFKNKADKGVRIQVEIPEIPVLALSPRDAFYSETEV 429

Query: 533 ENIKECVGKVCGELICLYPPGIPVMIPGEIISEEVVDYLLHLKGKGASISGASDPKLSSL 588
              +   G++  + + +YPPGIP+  PGEII+++ ++Y+      G  + G  D  L +L
Sbjct: 430 IPFENAAGRIIADFVMVYPPGIPIFTPGEIITQDNLEYIRKNLEAGLPVQGPEDMTLQTL 485

BLAST of Lsi06G012460 vs. ExPASy Swiss-Prot
Match: Q819L4 (Arginine decarboxylase OS=Bacillus cereus (strain ATCC 14579 / DSM 31 / JCM 2152 / NBRC 15305 / NCIMB 9373 / NRRL B-3711) OX=226900 GN=speA PE=3 SV=1)

HSP 1 Score: 292.0 bits (746), Expect = 1.5e-77
Identity = 162/448 (36.16%), Postives = 251/448 (56.03%), Query Frame = 0

Query: 113 PLVDALKVSSEQNAARFHFPGHNRGRAGPPSFTQLIGLKAFMHDLPELPELDNLFCPEGP 172
           PL  AL   S++N  +FH PGH +G+   P+F + IG  A   DL  +  LD+L  P+G 
Sbjct: 7   PLFTALVEHSKRNPIQFHIPGHKKGQGMDPTFREFIGHNALAIDLINIAPLDDLHHPKGM 66

Query: 173 ILEAQQQAAKLFGASETWFLVGGTTCGIQAAIMATCSPGDHIILPRNSHVSVISALVLSG 232
           I EAQ  AA  FGA  T+F + GT+  I   +M+ C PGD I++PRN H SV+SA++ SG
Sbjct: 67  IKEAQDLAAAAFGADHTFFSIQGTSGAIMTMVMSVCGPGDKILVPRNVHKSVMSAIIFSG 126

Query: 233 AIPKYIMPVYDSNWDIA-GEVDRAIKDLEMEGQKASAVFVTSPTYHGICSNLGEISQICH 292
           A P ++ P  D    I+ G   +++K    E   A  + V +PTY G  ++L +I Q+ H
Sbjct: 127 AKPIFMHPEIDPKLGISHGITIQSVKKALEEHSDAKGLLVINPTYFGFAADLEQIVQLAH 186

Query: 293 VKGIPFIVDEAHGAHFGFQPQLPLSALQQGVDLAVQSTHKVLYSLTQSSMLHMSGNIVDK 352
              IP +VDEAHG H  F  +LP+SA+Q G D+A  S HK+  SLTQSS+L++   +V+ 
Sbjct: 187 SYDIPVLVDEAHGVHIHFHDELPMSAMQAGADMAATSVHKLGGSLTQSSILNVKEGLVNV 246

Query: 353 ERICRCLQTLQSTSPSYLLLASLDAARAQLSDNPDKIFNRAIYLAYQAKRKINKIS---- 412
           + +   +  L +TS SY+LLASLD AR +L+     +  + I LA   +  IN I     
Sbjct: 247 KHVQSIISMLTTTSTSYILLASLDVARKRLATEGTALIEQTIQLAEHVRDAINSIEHLYC 306

Query: 413 -GVSILEFPMFSNFPAIDPLRLTIGFQQLGLSGYEADEILYKNHNIVCELVGNQSITFII 472
            G  +L      N+   DP ++ +  + LG++G++A+  L + +NI  EL    +I  +I
Sbjct: 307 PGKEMLGTDATFNY---DPTKIIVSVKDLGITGHQAEVWLREQYNIEVELSDLYNILCLI 366

Query: 473 NLGTSEDDIEILVSGIEDVSSFASIMRIEGRTKPIVSAPFPNVKI-SLNPRDAFFAKKRR 532
            LG +E D   L++ ++D+   A+  R        V    P + + +L+PRDAF+++   
Sbjct: 367 TLGDTESDTNTLIAALQDL---AATFRNRADKGVQVQVEIPEIPVLALSPRDAFYSETEV 426

Query: 533 ENIKECVGKVCGELICLYPPGIPVMIPG 554
              +   G++  + + +YPPGIP+  PG
Sbjct: 427 IPFENAAGRIIADFVMVYPPGIPIFTPG 448

BLAST of Lsi06G012460 vs. ExPASy Swiss-Prot
Match: P37536 (Uncharacterized protein YaaO OS=Bacillus subtilis (strain 168) OX=224308 GN=yaaO PE=3 SV=1)

HSP 1 Score: 241.5 bits (615), Expect = 2.4e-62
Identity = 165/486 (33.95%), Postives = 249/486 (51.23%), Query Frame = 0

Query: 113 PLVDALKVSSEQNAARFHFPGHNRGRA----GPPSFTQLIGLKAFMHDLPELPELDNLFC 172
           PL  AL   + +N+  FH PGH+ G          F  L+ +     D+ EL  LD+L  
Sbjct: 4   PLYKALIQHARRNSHSFHVPGHHNGDVFFDDAKSIFDPLLTI-----DVTELAGLDDLHH 63

Query: 173 PEGPILEAQQQAAKLFGASETWFLVGGTTCGIQAAIMATCSPGDHIILPRNSHVSVISAL 232
           P G I EAQ+ A++L+G++E++FLV GTT G  A I++ C PGD I++ RN H SV  A+
Sbjct: 64  PSGVIKEAQELASQLYGSAESFFLVNGTTVGNLAMILSVCEPGDTILVQRNCHKSVFHAV 123

Query: 233 VLSGAIPKYIMPVYDSNWDIAGEVD-RAIKDLEMEGQKASAVFVTSPTYHGICSNLGEIS 292
            LSGA P Y+ P  DS   +   V    IK+       A  + +T+PTY+G  ++L EI 
Sbjct: 124 DLSGAEPVYLAPDVDSAMHVPTHVPLGTIKEALEAYPDAKGLVLTNPTYYGHSADLTEII 183

Query: 293 QICHVKGIPFIVDEAHGAHFGFQPQLPLSALQQGVDLAVQSTHKVLYSLTQSSMLHMSGN 352
              H  GIP +VDEAHGAHF      P+SAL+ G D+ VQS HK L ++T  S LH++ +
Sbjct: 184 TEAHHYGIPVLVDEAHGAHFILGEPFPVSALKMGADIVVQSAHKTLPAMTMGSYLHLNSS 243

Query: 353 I-VDKERICRCLQTLQSTSPSYLLLASLDAARAQLSD-NPDKIFNRAIYLAYQAKRKINK 412
             ++++R+   L  LQS+SPSY ++ASLD ARA +     ++  +  +      K+  + 
Sbjct: 244 CRINRDRVAEYLNRLQSSSPSYPIMASLDIARAYVQHIIEEQKLSDILQRIETLKQTFDS 303

Query: 413 ISGVSILEFPMFSNFPAIDPLRLTIGFQQLGLSGYEADEILYKNHNIVCELVGNQSITFI 472
           ++    +     +     DPL+LTI  ++ G SGY    IL +  NI  EL     +  +
Sbjct: 304 LTNAEAVN--PANPLIITDPLKLTIRSKR-GHSGYTLQSIL-ERANIFTELADENQVLLV 363

Query: 473 INLG----TSEDDIEILVSGIEDVSSFASIMRIEGRTKPIVSAPFPNVKISLNPRDAFFA 532
           + LG     + + I  +   IE      + +  E   +P+   P+P        +     
Sbjct: 364 LPLGGKRRINAEIIRSIDEEIEKTPPDQTFVSAEWGVQPVTVLPYPK-------KVLHSF 423

Query: 533 KKRRENIKECVGKVCGELICLYPPGIPVMIPGEIISEEVVDYLLHLKGKGASISGASDPK 588
           KK   + +E  G++  E I  YPPGIP+++ GE I++E V  L  L      + G    K
Sbjct: 424 KKEYVSFEEAAGRLNAEDIIPYPPGIPMIMAGERITKESVQKLSRLISMKTHVQGNMKIK 473

BLAST of Lsi06G012460 vs. ExPASy TrEMBL
Match: A0A0A0LR51 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G424710 PE=3 SV=1)

HSP 1 Score: 941.4 bits (2432), Expect = 1.8e-270
Identity = 471/519 (90.75%), Postives = 490/519 (94.41%), Query Frame = 0

Query: 77  ETNIVEPKHNQDRSRKKQTTKKGIRESPISQEGSLPPLVDALKVSSEQNAARFHFPGHNR 136
           ETNIVEPKHN+DRSRKK  TKK IRES ISQEGS PPLV+ALKVS++Q+AARFHFPGHNR
Sbjct: 53  ETNIVEPKHNEDRSRKKHPTKKVIRESSISQEGSPPPLVNALKVSAQQDAARFHFPGHNR 112

Query: 137 GRAGPPSFTQLIGLKAFMHDLPELPELDNLFCPEGPILEAQQQAAKLFGASETWFLVGGT 196
           GRAGPPSFTQLIGLK FMHDLPELPELDNLFCPEGPILEAQQQAAKLFGASETWFLVGGT
Sbjct: 113 GRAGPPSFTQLIGLKPFMHDLPELPELDNLFCPEGPILEAQQQAAKLFGASETWFLVGGT 172

Query: 197 TCGIQAAIMATCSPGDHIILPRNSHVSVISALVLSGAIPKYIMPVYDSNWDIAG-----E 256
           TCGIQAAIMATCSPGDHIILPRNSHVSVISALVLSGAIPKYIMPVYDSNWDIAG     +
Sbjct: 173 TCGIQAAIMATCSPGDHIILPRNSHVSVISALVLSGAIPKYIMPVYDSNWDIAGVVTPSQ 232

Query: 257 VDRAIKDLEMEGQKASAVFVTSPTYHGICSNLGEISQICHVKGIPFIVDEAHGAHFGFQP 316
           + RAIKDLE+EGQKASAVFVTSPTYHGICSNL +ISQICHVKGIP IVDEAHGAHFGFQP
Sbjct: 233 IGRAIKDLEVEGQKASAVFVTSPTYHGICSNLSKISQICHVKGIPLIVDEAHGAHFGFQP 292

Query: 317 QLPLSALQQGVDLAVQSTHKVLYSLTQSSMLHMSGNIVDKERICRCLQTLQSTSPSYLLL 376
           QLPLSALQQG DLA QSTHKVL SLTQSSMLHMSGN+VD+ER+CRCLQTLQS+SPSYLLL
Sbjct: 293 QLPLSALQQGADLAAQSTHKVLCSLTQSSMLHMSGNLVDRERVCRCLQTLQSSSPSYLLL 352

Query: 377 ASLDAARAQLSDNPDKIFNRAIYLAYQAKRKINKISGVSILEFPMFSNFPAIDPLRLTIG 436
           ASLDAARAQLSDNPDK FNRAI LAYQAK KINKISG+SILE PM SNFPA+DPLRLTIG
Sbjct: 353 ASLDAARAQLSDNPDKTFNRAIDLAYQAKSKINKISGISILECPMLSNFPAVDPLRLTIG 412

Query: 437 FQQLGLSGYEADEILYKNHNIVCELVGNQSITFIINLGTSEDDIEILVSGIEDVSSFASI 496
           FQQLG+SGYEADEILYKNHNIVCELVGNQSITF+INLGTSEDDIE LVSGIEDVSSFAS+
Sbjct: 413 FQQLGISGYEADEILYKNHNIVCELVGNQSITFVINLGTSEDDIERLVSGIEDVSSFASV 472

Query: 497 MRIEGRTKPIVSAPFPNVKISLNPRDAFFAKKRRENIKECVGKVCGELICLYPPGIPVMI 556
            RIEGR+K  VSA FPNVKISLNPRDAFF KKRRENIKECVGKVCGELIC YPPGIPVMI
Sbjct: 473 QRIEGRSKLSVSALFPNVKISLNPRDAFFVKKRRENIKECVGKVCGELICPYPPGIPVMI 532

Query: 557 PGEIISEEVVDYLLHLKGKGASISGASDPKLSSLLVCNV 591
           PGEIISEEVVDYLLHLKGKGASISGASDPKLSSLLVCNV
Sbjct: 533 PGEIISEEVVDYLLHLKGKGASISGASDPKLSSLLVCNV 571

BLAST of Lsi06G012460 vs. ExPASy TrEMBL
Match: A0A5A7SNX5 (Arginine decarboxylase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold465G00290 PE=4 SV=1)

HSP 1 Score: 913.7 bits (2360), Expect = 4.0e-262
Identity = 465/558 (83.33%), Postives = 486/558 (87.10%), Query Frame = 0

Query: 77  ETNIVEPKHNQDRSRKKQTTKKGIRESPISQEGSLPPLVDALKVSSEQNAARFHFPGHNR 136
           ETNIVEPK NQDRSRKK  +KK IRE  ISQEGS PPLV+ALKVS++Q+AARFHFPGHNR
Sbjct: 53  ETNIVEPKPNQDRSRKKHRSKKAIREPSISQEGSPPPLVNALKVSAQQDAARFHFPGHNR 112

Query: 137 GRAGPPSFTQLIGLKAFMHDLPELPELDNLFCPEGPILEAQQQAAKLFGASETWFLVGGT 196
           GRAGPPSFTQLIGLK FMHDLPELPELDNLFCPEGPILEAQQQAAKLFGASETWFLVGGT
Sbjct: 113 GRAGPPSFTQLIGLKPFMHDLPELPELDNLFCPEGPILEAQQQAAKLFGASETWFLVGGT 172

Query: 197 TCGIQAAIMATCSPGDHIILPRNSHVSVISALVLSGAIPKYIMPVYDSNWDIAGE----- 256
           TCGIQAAIMATCSPGDHIILPRNSH+SVISALVLSGAIPKYIMPVYDSNWDIAGE     
Sbjct: 173 TCGIQAAIMATCSPGDHIILPRNSHISVISALVLSGAIPKYIMPVYDSNWDIAGEVTPSQ 232

Query: 257 ---------------------------------------VDRAIKDLEMEGQKASAVFVT 316
                                                  V RAIKDLE++GQKASAVFVT
Sbjct: 233 ASEEHLSSTHGLAIPLTIYNPPYCKRDNRWFDIILNAAQVGRAIKDLEIDGQKASAVFVT 292

Query: 317 SPTYHGICSNLGEISQICHVKGIPFIVDEAHGAHFGFQPQLPLSALQQGVDLAVQSTHKV 376
           SPTYHGICSNL EISQICHVKGIP IVDEAHGAHFGFQPQLPLSALQQGVDL  QSTHKV
Sbjct: 293 SPTYHGICSNLSEISQICHVKGIPLIVDEAHGAHFGFQPQLPLSALQQGVDLVAQSTHKV 352

Query: 377 LYSLTQSSMLHMSGNIVDKERICRCLQTLQSTSPSYLLLASLDAARAQLSDNPDKIFNRA 436
           L SLTQSSMLHMSGN++D+ER+CRCLQTLQS+SPSYLLLASLDAARAQLSDNPDKIFN A
Sbjct: 353 LCSLTQSSMLHMSGNLIDRERVCRCLQTLQSSSPSYLLLASLDAARAQLSDNPDKIFNTA 412

Query: 437 IYLAYQAKRKINKISGVSILEFPMFSNFPAIDPLRLTIGFQQLGLSGYEADEILYKNHNI 496
           I LAYQAK +INKISG+SILE  MFSNFP +DPLRLTIGFQQLG+SGYEADEILYKNHNI
Sbjct: 413 IDLAYQAKSEINKISGISILECAMFSNFPVVDPLRLTIGFQQLGISGYEADEILYKNHNI 472

Query: 497 VCELVGNQSITFIINLGTSEDDIEILVSGIEDVSSFASIMRIEGRTKPIVSAPFPNVKIS 556
           VCELVGNQ ITF+INLGTSEDDIE LV GIE+VSSFAS+MRIEGR+K  VSA FPNVKIS
Sbjct: 473 VCELVGNQFITFVINLGTSEDDIERLVLGIENVSSFASVMRIEGRSKLSVSALFPNVKIS 532

Query: 557 LNPRDAFFAKKRRENIKECVGKVCGELICLYPPGIPVMIPGEIISEEVVDYLLHLKGKGA 591
           LNPRDAFF KKRRENIKECVGKVCGELIC YPPGIPVMIPGEIISEEVVDYLLHLKGKGA
Sbjct: 533 LNPRDAFFVKKRRENIKECVGKVCGELICPYPPGIPVMIPGEIISEEVVDYLLHLKGKGA 592

BLAST of Lsi06G012460 vs. ExPASy TrEMBL
Match: A0A6J1JG27 (uncharacterized protein LOC111484796 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111484796 PE=3 SV=1)

HSP 1 Score: 911.8 bits (2355), Expect = 1.5e-261
Identity = 458/520 (88.08%), Postives = 484/520 (93.08%), Query Frame = 0

Query: 77  ETNIVEPKHNQDRSRKKQTTKKGIRESPISQEGSLP-PLVDALKVSSEQNAARFHFPGHN 136
           ET+IV+PK+NQD   KKQT+ K I ESPISQEGS   PLV+ALKVS+E++AARFHFPGHN
Sbjct: 40  ETSIVKPKNNQDGPSKKQTSTKAIGESPISQEGSRTLPLVNALKVSAEKDAARFHFPGHN 99

Query: 137 RGRAGPPSFTQLIGLKAFMHDLPELPELDNLFCPEGPILEAQQQAAKLFGASETWFLVGG 196
            GRA P SFTQLIGLK FMHDLPELPELDNLFCPEGPILEAQQQAAKLFGASETWFLVGG
Sbjct: 100 GGRAAPSSFTQLIGLKPFMHDLPELPELDNLFCPEGPILEAQQQAAKLFGASETWFLVGG 159

Query: 197 TTCGIQAAIMATCSPGDHIILPRNSHVSVISALVLSGAIPKYIMPVYDSNWDIAG----- 256
           TTCGIQAAIMATCSPGDHIILPRNSH+SVISALV+SGAIPKYIMP YDSNWDIAG     
Sbjct: 160 TTCGIQAAIMATCSPGDHIILPRNSHISVISALVISGAIPKYIMPEYDSNWDIAGGVTPS 219

Query: 257 EVDRAIKDLEMEGQKASAVFVTSPTYHGICSNLGEISQICHVKGIPFIVDEAHGAHFGFQ 316
           +VDR IKDLEMEGQKASAV VTSPTYHGICS+L EISQICH KGIP IVDEAHGAHFGFQ
Sbjct: 220 QVDREIKDLEMEGQKASAVLVTSPTYHGICSDLREISQICHAKGIPLIVDEAHGAHFGFQ 279

Query: 317 PQLPLSALQQGVDLAVQSTHKVLYSLTQSSMLHMSGNIVDKERICRCLQTLQSTSPSYLL 376
           PQLPLSALQQG DLAVQSTHKVL SLTQSSMLHMSGNI+D+E ICRCLQTLQSTSPSYLL
Sbjct: 280 PQLPLSALQQGADLAVQSTHKVLCSLTQSSMLHMSGNIIDREMICRCLQTLQSTSPSYLL 339

Query: 377 LASLDAARAQLSDNPDKIFNRAIYLAYQAKRKINKISGVSILEFPMFSNFPAIDPLRLTI 436
           LASLDAARAQLSDNPDKIFNRAI LAYQAKRK+NK SG+SIL+FPMFSNFPAIDPLRLTI
Sbjct: 340 LASLDAARAQLSDNPDKIFNRAIDLAYQAKRKMNKTSGISILKFPMFSNFPAIDPLRLTI 399

Query: 437 GFQQLGLSGYEADEILYKNHNIVCELVGNQSITFIINLGTSEDDIEILVSGIEDVSSFAS 496
           GFQQLGLSGYEADE +YKNHNIVCELVGNQSITF+INLGT EDDIE LVSGI+DVSSFAS
Sbjct: 400 GFQQLGLSGYEADETIYKNHNIVCELVGNQSITFVINLGTCEDDIERLVSGIDDVSSFAS 459

Query: 497 IMRIEGRTKPIVSAPFPNVKISLNPRDAFFAKKRRENIKECVGKVCGELICLYPPGIPVM 556
           I+RIEGR+K  VSAPFPNVKI+LNPRDAFF+KKRRENIKECVGKVCGEL+C YPPGIPVM
Sbjct: 460 ILRIEGRSKFSVSAPFPNVKINLNPRDAFFSKKRRENIKECVGKVCGELVCPYPPGIPVM 519

Query: 557 IPGEIISEEVVDYLLHLKGKGASISGASDPKLSSLLVCNV 591
           IPGEIISEEV+DYLLHLK KGASISGASDPKLSSLLVCNV
Sbjct: 520 IPGEIISEEVLDYLLHLKSKGASISGASDPKLSSLLVCNV 559

BLAST of Lsi06G012460 vs. ExPASy TrEMBL
Match: A0A6J1JDD3 (uncharacterized protein LOC111484796 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111484796 PE=3 SV=1)

HSP 1 Score: 911.8 bits (2355), Expect = 1.5e-261
Identity = 458/520 (88.08%), Postives = 484/520 (93.08%), Query Frame = 0

Query: 77  ETNIVEPKHNQDRSRKKQTTKKGIRESPISQEGSLP-PLVDALKVSSEQNAARFHFPGHN 136
           ET+IV+PK+NQD   KKQT+ K I ESPISQEGS   PLV+ALKVS+E++AARFHFPGHN
Sbjct: 39  ETSIVKPKNNQDGPSKKQTSTKAIGESPISQEGSRTLPLVNALKVSAEKDAARFHFPGHN 98

Query: 137 RGRAGPPSFTQLIGLKAFMHDLPELPELDNLFCPEGPILEAQQQAAKLFGASETWFLVGG 196
            GRA P SFTQLIGLK FMHDLPELPELDNLFCPEGPILEAQQQAAKLFGASETWFLVGG
Sbjct: 99  GGRAAPSSFTQLIGLKPFMHDLPELPELDNLFCPEGPILEAQQQAAKLFGASETWFLVGG 158

Query: 197 TTCGIQAAIMATCSPGDHIILPRNSHVSVISALVLSGAIPKYIMPVYDSNWDIAG----- 256
           TTCGIQAAIMATCSPGDHIILPRNSH+SVISALV+SGAIPKYIMP YDSNWDIAG     
Sbjct: 159 TTCGIQAAIMATCSPGDHIILPRNSHISVISALVISGAIPKYIMPEYDSNWDIAGGVTPS 218

Query: 257 EVDRAIKDLEMEGQKASAVFVTSPTYHGICSNLGEISQICHVKGIPFIVDEAHGAHFGFQ 316
           +VDR IKDLEMEGQKASAV VTSPTYHGICS+L EISQICH KGIP IVDEAHGAHFGFQ
Sbjct: 219 QVDREIKDLEMEGQKASAVLVTSPTYHGICSDLREISQICHAKGIPLIVDEAHGAHFGFQ 278

Query: 317 PQLPLSALQQGVDLAVQSTHKVLYSLTQSSMLHMSGNIVDKERICRCLQTLQSTSPSYLL 376
           PQLPLSALQQG DLAVQSTHKVL SLTQSSMLHMSGNI+D+E ICRCLQTLQSTSPSYLL
Sbjct: 279 PQLPLSALQQGADLAVQSTHKVLCSLTQSSMLHMSGNIIDREMICRCLQTLQSTSPSYLL 338

Query: 377 LASLDAARAQLSDNPDKIFNRAIYLAYQAKRKINKISGVSILEFPMFSNFPAIDPLRLTI 436
           LASLDAARAQLSDNPDKIFNRAI LAYQAKRK+NK SG+SIL+FPMFSNFPAIDPLRLTI
Sbjct: 339 LASLDAARAQLSDNPDKIFNRAIDLAYQAKRKMNKTSGISILKFPMFSNFPAIDPLRLTI 398

Query: 437 GFQQLGLSGYEADEILYKNHNIVCELVGNQSITFIINLGTSEDDIEILVSGIEDVSSFAS 496
           GFQQLGLSGYEADE +YKNHNIVCELVGNQSITF+INLGT EDDIE LVSGI+DVSSFAS
Sbjct: 399 GFQQLGLSGYEADETIYKNHNIVCELVGNQSITFVINLGTCEDDIERLVSGIDDVSSFAS 458

Query: 497 IMRIEGRTKPIVSAPFPNVKISLNPRDAFFAKKRRENIKECVGKVCGELICLYPPGIPVM 556
           I+RIEGR+K  VSAPFPNVKI+LNPRDAFF+KKRRENIKECVGKVCGEL+C YPPGIPVM
Sbjct: 459 ILRIEGRSKFSVSAPFPNVKINLNPRDAFFSKKRRENIKECVGKVCGELVCPYPPGIPVM 518

Query: 557 IPGEIISEEVVDYLLHLKGKGASISGASDPKLSSLLVCNV 591
           IPGEIISEEV+DYLLHLK KGASISGASDPKLSSLLVCNV
Sbjct: 519 IPGEIISEEVLDYLLHLKSKGASISGASDPKLSSLLVCNV 558

BLAST of Lsi06G012460 vs. ExPASy TrEMBL
Match: A0A6J1E1Q8 (uncharacterized protein LOC111429785 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111429785 PE=3 SV=1)

HSP 1 Score: 911.8 bits (2355), Expect = 1.5e-261
Identity = 459/520 (88.27%), Postives = 484/520 (93.08%), Query Frame = 0

Query: 77  ETNIVEPKHNQDRSRKKQTTKKGIRESPISQEGSLP-PLVDALKVSSEQNAARFHFPGHN 136
           ET+IV+PK+NQD S KKQT+ K I ESPISQEGS   PLV+ALKVS+E++AARFHFPGHN
Sbjct: 39  ETSIVKPKNNQDGSSKKQTSTKAIGESPISQEGSSTLPLVNALKVSAEKDAARFHFPGHN 98

Query: 137 RGRAGPPSFTQLIGLKAFMHDLPELPELDNLFCPEGPILEAQQQAAKLFGASETWFLVGG 196
            GRA P SFTQLIGLK FMHDLPELPELDNLFCPEGPILEAQQQAAKLFGASETWFLVGG
Sbjct: 99  GGRAAPSSFTQLIGLKPFMHDLPELPELDNLFCPEGPILEAQQQAAKLFGASETWFLVGG 158

Query: 197 TTCGIQAAIMATCSPGDHIILPRNSHVSVISALVLSGAIPKYIMPVYDSNWDIAG----- 256
           TTCGIQAAIMATCSPGDHIILPRNSH+SVISALV+SGAIPKYIMP YDSNWDIAG     
Sbjct: 159 TTCGIQAAIMATCSPGDHIILPRNSHISVISALVISGAIPKYIMPEYDSNWDIAGGVTPS 218

Query: 257 EVDRAIKDLEMEGQKASAVFVTSPTYHGICSNLGEISQICHVKGIPFIVDEAHGAHFGFQ 316
           +VDRAI+DLEMEGQKASAV VTSPTYHGICS+L EISQICH KGIP IVDEAHGAHFGFQ
Sbjct: 219 QVDRAIEDLEMEGQKASAVLVTSPTYHGICSDLREISQICHAKGIPLIVDEAHGAHFGFQ 278

Query: 317 PQLPLSALQQGVDLAVQSTHKVLYSLTQSSMLHMSGNIVDKERICRCLQTLQSTSPSYLL 376
           PQLPLSALQQG DLAVQSTHKVL SLTQSSMLHMSGNI+D+E +CRCLQTLQSTSPSYLL
Sbjct: 279 PQLPLSALQQGADLAVQSTHKVLCSLTQSSMLHMSGNIIDREMVCRCLQTLQSTSPSYLL 338

Query: 377 LASLDAARAQLSDNPDKIFNRAIYLAYQAKRKINKISGVSILEFPMFSNFPAIDPLRLTI 436
           LASLDAARAQLSDNPDKIFNRAI LAYQAK K+NKISG+SILEFPMFSNFPAIDPLRLTI
Sbjct: 339 LASLDAARAQLSDNPDKIFNRAIDLAYQAKSKMNKISGISILEFPMFSNFPAIDPLRLTI 398

Query: 437 GFQQLGLSGYEADEILYKNHNIVCELVGNQSITFIINLGTSEDDIEILVSGIEDVSSFAS 496
           GFQQLGLSGYEAD  +YKNHNIVCELVGNQSITF+INLGT EDDIE LVSGI+DVSSFAS
Sbjct: 399 GFQQLGLSGYEADATIYKNHNIVCELVGNQSITFVINLGTCEDDIERLVSGIDDVSSFAS 458

Query: 497 IMRIEGRTKPIVSAPFPNVKISLNPRDAFFAKKRRENIKECVGKVCGELICLYPPGIPVM 556
           I+RIEGR+K  VSAPFPNVKISLNPRDAFF+KKRRENIKECVGKVCGELIC YPPGIPVM
Sbjct: 459 ILRIEGRSKFSVSAPFPNVKISLNPRDAFFSKKRRENIKECVGKVCGELICPYPPGIPVM 518

Query: 557 IPGEIISEEVVDYLLHLKGKGASISGASDPKLSSLLVCNV 591
           IPGEIISEEV+DYLLHLK KGASISGASDPKL SLLVCNV
Sbjct: 519 IPGEIISEEVLDYLLHLKSKGASISGASDPKLYSLLVCNV 558

BLAST of Lsi06G012460 vs. NCBI nr
Match: XP_011650023.1 (uncharacterized protein LOC101211215 [Cucumis sativus] >KGN63292.1 hypothetical protein Csa_022366 [Cucumis sativus])

HSP 1 Score: 941.4 bits (2432), Expect = 3.7e-270
Identity = 471/519 (90.75%), Postives = 490/519 (94.41%), Query Frame = 0

Query: 77  ETNIVEPKHNQDRSRKKQTTKKGIRESPISQEGSLPPLVDALKVSSEQNAARFHFPGHNR 136
           ETNIVEPKHN+DRSRKK  TKK IRES ISQEGS PPLV+ALKVS++Q+AARFHFPGHNR
Sbjct: 53  ETNIVEPKHNEDRSRKKHPTKKVIRESSISQEGSPPPLVNALKVSAQQDAARFHFPGHNR 112

Query: 137 GRAGPPSFTQLIGLKAFMHDLPELPELDNLFCPEGPILEAQQQAAKLFGASETWFLVGGT 196
           GRAGPPSFTQLIGLK FMHDLPELPELDNLFCPEGPILEAQQQAAKLFGASETWFLVGGT
Sbjct: 113 GRAGPPSFTQLIGLKPFMHDLPELPELDNLFCPEGPILEAQQQAAKLFGASETWFLVGGT 172

Query: 197 TCGIQAAIMATCSPGDHIILPRNSHVSVISALVLSGAIPKYIMPVYDSNWDIAG-----E 256
           TCGIQAAIMATCSPGDHIILPRNSHVSVISALVLSGAIPKYIMPVYDSNWDIAG     +
Sbjct: 173 TCGIQAAIMATCSPGDHIILPRNSHVSVISALVLSGAIPKYIMPVYDSNWDIAGVVTPSQ 232

Query: 257 VDRAIKDLEMEGQKASAVFVTSPTYHGICSNLGEISQICHVKGIPFIVDEAHGAHFGFQP 316
           + RAIKDLE+EGQKASAVFVTSPTYHGICSNL +ISQICHVKGIP IVDEAHGAHFGFQP
Sbjct: 233 IGRAIKDLEVEGQKASAVFVTSPTYHGICSNLSKISQICHVKGIPLIVDEAHGAHFGFQP 292

Query: 317 QLPLSALQQGVDLAVQSTHKVLYSLTQSSMLHMSGNIVDKERICRCLQTLQSTSPSYLLL 376
           QLPLSALQQG DLA QSTHKVL SLTQSSMLHMSGN+VD+ER+CRCLQTLQS+SPSYLLL
Sbjct: 293 QLPLSALQQGADLAAQSTHKVLCSLTQSSMLHMSGNLVDRERVCRCLQTLQSSSPSYLLL 352

Query: 377 ASLDAARAQLSDNPDKIFNRAIYLAYQAKRKINKISGVSILEFPMFSNFPAIDPLRLTIG 436
           ASLDAARAQLSDNPDK FNRAI LAYQAK KINKISG+SILE PM SNFPA+DPLRLTIG
Sbjct: 353 ASLDAARAQLSDNPDKTFNRAIDLAYQAKSKINKISGISILECPMLSNFPAVDPLRLTIG 412

Query: 437 FQQLGLSGYEADEILYKNHNIVCELVGNQSITFIINLGTSEDDIEILVSGIEDVSSFASI 496
           FQQLG+SGYEADEILYKNHNIVCELVGNQSITF+INLGTSEDDIE LVSGIEDVSSFAS+
Sbjct: 413 FQQLGISGYEADEILYKNHNIVCELVGNQSITFVINLGTSEDDIERLVSGIEDVSSFASV 472

Query: 497 MRIEGRTKPIVSAPFPNVKISLNPRDAFFAKKRRENIKECVGKVCGELICLYPPGIPVMI 556
            RIEGR+K  VSA FPNVKISLNPRDAFF KKRRENIKECVGKVCGELIC YPPGIPVMI
Sbjct: 473 QRIEGRSKLSVSALFPNVKISLNPRDAFFVKKRRENIKECVGKVCGELICPYPPGIPVMI 532

Query: 557 PGEIISEEVVDYLLHLKGKGASISGASDPKLSSLLVCNV 591
           PGEIISEEVVDYLLHLKGKGASISGASDPKLSSLLVCNV
Sbjct: 533 PGEIISEEVVDYLLHLKGKGASISGASDPKLSSLLVCNV 571

BLAST of Lsi06G012460 vs. NCBI nr
Match: XP_038878280.1 (arginine decarboxylase-like [Benincasa hispida])

HSP 1 Score: 938.3 bits (2424), Expect = 3.1e-269
Identity = 472/519 (90.94%), Postives = 489/519 (94.22%), Query Frame = 0

Query: 77  ETNIVEPKHNQDRSRKKQTTKKGIRESPISQEGSLPPLVDALKVSSEQNAARFHFPGHNR 136
           E NIVEPKHNQDRSRKKQTT+K IR  PISQEGS PPLV+ALKVS+EQNAARFHFPGHNR
Sbjct: 40  EANIVEPKHNQDRSRKKQTTEKAIRVPPISQEGSPPPLVNALKVSAEQNAARFHFPGHNR 99

Query: 137 GRAGPPSFTQLIGLKAFMHDLPELPELDNLFCPEGPILEAQQQAAKLFGASETWFLVGGT 196
           GRAGPPSFTQLIGLK F+HDLPELPELDNLFCPEGPILEAQQQAAKLFGASETWFLVGGT
Sbjct: 100 GRAGPPSFTQLIGLKPFVHDLPELPELDNLFCPEGPILEAQQQAAKLFGASETWFLVGGT 159

Query: 197 TCGIQAAIMATCSPGDHIILPRNSHVSVISALVLSGAIPKYIMPVYDSNWDIAGE----- 256
           TCGIQAAIMATCSPGDHIILPRNSHVSVISALVLSGAIPKYIMPVYDSNWDIAGE     
Sbjct: 160 TCGIQAAIMATCSPGDHIILPRNSHVSVISALVLSGAIPKYIMPVYDSNWDIAGEVTPSQ 219

Query: 257 VDRAIKDLEMEGQKASAVFVTSPTYHGICSNLGEISQICHVKGIPFIVDEAHGAHFGFQP 316
           VD+AIKDLEM+GQKASAVFVTSPTYHGICSNL EISQICHVKGIPFIVDEAHGAHFGFQP
Sbjct: 220 VDKAIKDLEMQGQKASAVFVTSPTYHGICSNLSEISQICHVKGIPFIVDEAHGAHFGFQP 279

Query: 317 QLPLSALQQGVDLAVQSTHKVLYSLTQSSMLHMSGNIVDKERICRCLQTLQSTSPSYLLL 376
           +LPLSALQQG DLA QSTHKVL SLTQSSMLHMSGNIVD+ER+CRCLQTLQSTSPSYLLL
Sbjct: 280 ELPLSALQQGADLAAQSTHKVLCSLTQSSMLHMSGNIVDRERVCRCLQTLQSTSPSYLLL 339

Query: 377 ASLDAARAQLSDNPDKIFNRAIYLAYQAKRKINKISGVSILEFPMFSNFPAIDPLRLTIG 436
           ASLDAARAQLSDNPDKIFNRAI LAYQAK KINKISG+SIL+FP  +NFPAIDPLRLTIG
Sbjct: 340 ASLDAARAQLSDNPDKIFNRAINLAYQAKSKINKISGISILKFPPVTNFPAIDPLRLTIG 399

Query: 437 FQQLGLSGYEADEILYKNHNIVCELVGNQSITFIINLGTSEDDIEILVSGIEDVSSFASI 496
           FQQLGLSGYEADEILYKNH+IVCELVGNQSITF+INLGTSEDDIE LV GIEDV SFASI
Sbjct: 400 FQQLGLSGYEADEILYKNHDIVCELVGNQSITFVINLGTSEDDIERLVPGIEDVCSFASI 459

Query: 497 MRIEGRTKPIVSAPFPNVKISLNPRDAFFAKKRRENIKECVGKVCGELICLYPPGIPVMI 556
           + IE R+K  VSAPFPNVKISLNPRDAFFAKKRRENIKECVGKV GELIC YPPGIPVMI
Sbjct: 460 LGIEERSKLNVSAPFPNVKISLNPRDAFFAKKRRENIKECVGKVSGELICPYPPGIPVMI 519

Query: 557 PGEIISEEVVDYLLHLKGKGASISGASDPKLSSLLVCNV 591
           PGE ISEEVVDYLLHLKG GASISGASDPKLSSLLVCN+
Sbjct: 520 PGETISEEVVDYLLHLKGIGASISGASDPKLSSLLVCNL 558

BLAST of Lsi06G012460 vs. NCBI nr
Match: KAG7023342.1 (speA, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 921.4 bits (2380), Expect = 4.0e-264
Identity = 460/515 (89.32%), Postives = 485/515 (94.17%), Query Frame = 0

Query: 77  ETNIVEPKHNQDRSRKKQTTKKGIRESPISQEGSLP-PLVDALKVSSEQNAARFHFPGHN 136
           ET+IV+PK+NQD S KKQT+ K I ESPISQEGS   PLV+ALKVS+E++AARFHFPGHN
Sbjct: 67  ETSIVKPKNNQDGSSKKQTSTKAIGESPISQEGSSTLPLVNALKVSAEKDAARFHFPGHN 126

Query: 137 RGRAGPPSFTQLIGLKAFMHDLPELPELDNLFCPEGPILEAQQQAAKLFGASETWFLVGG 196
            GRA P SFTQLIGLK FMHDLPELPELDNLFCPEGPILEAQQQAAKLFGASETWFLVGG
Sbjct: 127 GGRAAPSSFTQLIGLKPFMHDLPELPELDNLFCPEGPILEAQQQAAKLFGASETWFLVGG 186

Query: 197 TTCGIQAAIMATCSPGDHIILPRNSHVSVISALVLSGAIPKYIMPVYDSNWDIAGEVDRA 256
           TTCGIQAAIMATCSPGDHIILPRNSH+SVISALV+SGAIPKYIMP YDSNWDIAG VDRA
Sbjct: 187 TTCGIQAAIMATCSPGDHIILPRNSHISVISALVISGAIPKYIMPEYDSNWDIAGGVDRA 246

Query: 257 IKDLEMEGQKASAVFVTSPTYHGICSNLGEISQICHVKGIPFIVDEAHGAHFGFQPQLPL 316
           I+DLEMEGQKASAV VTSPTYHGICS+L EISQICH KGIP IVDEAHGAHFGFQPQLPL
Sbjct: 247 IEDLEMEGQKASAVLVTSPTYHGICSDLREISQICHAKGIPLIVDEAHGAHFGFQPQLPL 306

Query: 317 SALQQGVDLAVQSTHKVLYSLTQSSMLHMSGNIVDKERICRCLQTLQSTSPSYLLLASLD 376
           SALQQG DLAVQSTHKVL SLTQSSMLHMSGNI+D+E +CRCLQTLQSTSPSYLLLASLD
Sbjct: 307 SALQQGADLAVQSTHKVLCSLTQSSMLHMSGNIIDREMVCRCLQTLQSTSPSYLLLASLD 366

Query: 377 AARAQLSDNPDKIFNRAIYLAYQAKRKINKISGVSILEFPMFSNFPAIDPLRLTIGFQQL 436
           AARAQLSDNPDKIFNRAI LAYQAK K+NKISG+SILEFPMFSNFPAIDPLRLTIGFQQL
Sbjct: 367 AARAQLSDNPDKIFNRAIDLAYQAKSKMNKISGISILEFPMFSNFPAIDPLRLTIGFQQL 426

Query: 437 GLSGYEADEILYKNHNIVCELVGNQSITFIINLGTSEDDIEILVSGIEDVSSFASIMRIE 496
           GLSGYEADE +YKNHNIVCELVGNQSITF+INLGT EDDIE LVSGI+DVSSFASI+RIE
Sbjct: 427 GLSGYEADETIYKNHNIVCELVGNQSITFVINLGTCEDDIERLVSGIDDVSSFASILRIE 486

Query: 497 GRTKPIVSAPFPNVKISLNPRDAFFAKKRRENIKECVGKVCGELICLYPPGIPVMIPGEI 556
           GR+K  VSAPFPNVKISLNPRDAFF+KKRRENIKECVGKVCGELIC YPPGIPVMIPGEI
Sbjct: 487 GRSKVTVSAPFPNVKISLNPRDAFFSKKRRENIKECVGKVCGELICPYPPGIPVMIPGEI 546

Query: 557 ISEEVVDYLLHLKGKGASISGASDPKLSSLLVCNV 591
           ISEEV+DYLLHLK KGAS+SGASDPKLSSLLVCNV
Sbjct: 547 ISEEVLDYLLHLKSKGASLSGASDPKLSSLLVCNV 581

BLAST of Lsi06G012460 vs. NCBI nr
Match: KAG6589654.1 (hypothetical protein SDJN03_15077, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 916.8 bits (2368), Expect = 9.8e-263
Identity = 460/520 (88.46%), Postives = 486/520 (93.46%), Query Frame = 0

Query: 77  ETNIVEPKHNQDRSRKKQTTKKGIRESPISQEGSLP-PLVDALKVSSEQNAARFHFPGHN 136
           ET+IV+PK+NQD S KKQT+ K I ESPISQEGS   PLV+ALKVS+E++AARFHFPGHN
Sbjct: 40  ETSIVKPKNNQDGSSKKQTSTKAIGESPISQEGSSTLPLVNALKVSAEKDAARFHFPGHN 99

Query: 137 RGRAGPPSFTQLIGLKAFMHDLPELPELDNLFCPEGPILEAQQQAAKLFGASETWFLVGG 196
            GRA P SFTQLIGLK FMHDLPELPELDNLFCPEGPILEAQQQAAKLFGASETWFLVGG
Sbjct: 100 GGRAAPSSFTQLIGLKPFMHDLPELPELDNLFCPEGPILEAQQQAAKLFGASETWFLVGG 159

Query: 197 TTCGIQAAIMATCSPGDHIILPRNSHVSVISALVLSGAIPKYIMPVYDSNWDIAG----- 256
           TTCGIQAAIMATCSPGDHIILPRNSH+SVISALV+SGAIPKYIMP YDSNWDIAG     
Sbjct: 160 TTCGIQAAIMATCSPGDHIILPRNSHISVISALVISGAIPKYIMPEYDSNWDIAGGVTPS 219

Query: 257 EVDRAIKDLEMEGQKASAVFVTSPTYHGICSNLGEISQICHVKGIPFIVDEAHGAHFGFQ 316
           +VDRAI+DLEMEGQKASAV VTSPTYHGICS+L EISQICH KGIP IVDEAHGAHFGFQ
Sbjct: 220 QVDRAIEDLEMEGQKASAVLVTSPTYHGICSDLREISQICHAKGIPLIVDEAHGAHFGFQ 279

Query: 317 PQLPLSALQQGVDLAVQSTHKVLYSLTQSSMLHMSGNIVDKERICRCLQTLQSTSPSYLL 376
           PQLPLSALQQG DLAVQSTHKVL SLTQSSMLHMSGNI+D+E +CRCLQTLQSTSPSYLL
Sbjct: 280 PQLPLSALQQGADLAVQSTHKVLCSLTQSSMLHMSGNIIDREMVCRCLQTLQSTSPSYLL 339

Query: 377 LASLDAARAQLSDNPDKIFNRAIYLAYQAKRKINKISGVSILEFPMFSNFPAIDPLRLTI 436
           LASLDAARAQLSDNPDKIFNRAI LAYQAK K+NKISG+SILEFPMFSNFPAIDPLRLTI
Sbjct: 340 LASLDAARAQLSDNPDKIFNRAIDLAYQAKSKMNKISGISILEFPMFSNFPAIDPLRLTI 399

Query: 437 GFQQLGLSGYEADEILYKNHNIVCELVGNQSITFIINLGTSEDDIEILVSGIEDVSSFAS 496
           GFQQLGLSGYEADE +YKNHNIVCELVGNQSITF+INLGT EDDIE LVSGI+DVSSFAS
Sbjct: 400 GFQQLGLSGYEADETIYKNHNIVCELVGNQSITFVINLGTCEDDIERLVSGIDDVSSFAS 459

Query: 497 IMRIEGRTKPIVSAPFPNVKISLNPRDAFFAKKRRENIKECVGKVCGELICLYPPGIPVM 556
           I+RIEGR+K  VSAPFPNVKISLNPRDAFF+KKRRENIKECVGKVCGELIC YPPGIPVM
Sbjct: 460 ILRIEGRSKVTVSAPFPNVKISLNPRDAFFSKKRRENIKECVGKVCGELICPYPPGIPVM 519

Query: 557 IPGEIISEEVVDYLLHLKGKGASISGASDPKLSSLLVCNV 591
           IPGEIISEEV+DYLLHLK KGAS+SGASDPKLSSLLVCNV
Sbjct: 520 IPGEIISEEVLDYLLHLKSKGASLSGASDPKLSSLLVCNV 559

BLAST of Lsi06G012460 vs. NCBI nr
Match: KAA0032520.1 (arginine decarboxylase-like [Cucumis melo var. makuwa])

HSP 1 Score: 913.7 bits (2360), Expect = 8.3e-262
Identity = 465/558 (83.33%), Postives = 486/558 (87.10%), Query Frame = 0

Query: 77  ETNIVEPKHNQDRSRKKQTTKKGIRESPISQEGSLPPLVDALKVSSEQNAARFHFPGHNR 136
           ETNIVEPK NQDRSRKK  +KK IRE  ISQEGS PPLV+ALKVS++Q+AARFHFPGHNR
Sbjct: 53  ETNIVEPKPNQDRSRKKHRSKKAIREPSISQEGSPPPLVNALKVSAQQDAARFHFPGHNR 112

Query: 137 GRAGPPSFTQLIGLKAFMHDLPELPELDNLFCPEGPILEAQQQAAKLFGASETWFLVGGT 196
           GRAGPPSFTQLIGLK FMHDLPELPELDNLFCPEGPILEAQQQAAKLFGASETWFLVGGT
Sbjct: 113 GRAGPPSFTQLIGLKPFMHDLPELPELDNLFCPEGPILEAQQQAAKLFGASETWFLVGGT 172

Query: 197 TCGIQAAIMATCSPGDHIILPRNSHVSVISALVLSGAIPKYIMPVYDSNWDIAGE----- 256
           TCGIQAAIMATCSPGDHIILPRNSH+SVISALVLSGAIPKYIMPVYDSNWDIAGE     
Sbjct: 173 TCGIQAAIMATCSPGDHIILPRNSHISVISALVLSGAIPKYIMPVYDSNWDIAGEVTPSQ 232

Query: 257 ---------------------------------------VDRAIKDLEMEGQKASAVFVT 316
                                                  V RAIKDLE++GQKASAVFVT
Sbjct: 233 ASEEHLSSTHGLAIPLTIYNPPYCKRDNRWFDIILNAAQVGRAIKDLEIDGQKASAVFVT 292

Query: 317 SPTYHGICSNLGEISQICHVKGIPFIVDEAHGAHFGFQPQLPLSALQQGVDLAVQSTHKV 376
           SPTYHGICSNL EISQICHVKGIP IVDEAHGAHFGFQPQLPLSALQQGVDL  QSTHKV
Sbjct: 293 SPTYHGICSNLSEISQICHVKGIPLIVDEAHGAHFGFQPQLPLSALQQGVDLVAQSTHKV 352

Query: 377 LYSLTQSSMLHMSGNIVDKERICRCLQTLQSTSPSYLLLASLDAARAQLSDNPDKIFNRA 436
           L SLTQSSMLHMSGN++D+ER+CRCLQTLQS+SPSYLLLASLDAARAQLSDNPDKIFN A
Sbjct: 353 LCSLTQSSMLHMSGNLIDRERVCRCLQTLQSSSPSYLLLASLDAARAQLSDNPDKIFNTA 412

Query: 437 IYLAYQAKRKINKISGVSILEFPMFSNFPAIDPLRLTIGFQQLGLSGYEADEILYKNHNI 496
           I LAYQAK +INKISG+SILE  MFSNFP +DPLRLTIGFQQLG+SGYEADEILYKNHNI
Sbjct: 413 IDLAYQAKSEINKISGISILECAMFSNFPVVDPLRLTIGFQQLGISGYEADEILYKNHNI 472

Query: 497 VCELVGNQSITFIINLGTSEDDIEILVSGIEDVSSFASIMRIEGRTKPIVSAPFPNVKIS 556
           VCELVGNQ ITF+INLGTSEDDIE LV GIE+VSSFAS+MRIEGR+K  VSA FPNVKIS
Sbjct: 473 VCELVGNQFITFVINLGTSEDDIERLVLGIENVSSFASVMRIEGRSKLSVSALFPNVKIS 532

Query: 557 LNPRDAFFAKKRRENIKECVGKVCGELICLYPPGIPVMIPGEIISEEVVDYLLHLKGKGA 591
           LNPRDAFF KKRRENIKECVGKVCGELIC YPPGIPVMIPGEIISEEVVDYLLHLKGKGA
Sbjct: 533 LNPRDAFFVKKRRENIKECVGKVCGELICPYPPGIPVMIPGEIISEEVVDYLLHLKGKGA 592