Lsi06G011330 (gene) Bottle gourd (USVL1VR-Ls) v1

BLAST of Lsi06G011330 vs. ExPASy Swiss-Prot
Match: M4ISH0 (4-coumarate--CoA ligase CCL1 OS=Humulus lupulus OX=3486 GN=CCL1 PE=1 SV=1)

HSP 1 Score: 733.4 bits (1892), Expect = 2.1e-210
Identity = 370/561 (65.95%), Postives = 443/561 (78.97%), Query Frame = 0

Query: 44  DDPSLEFIFRSKLPQIPIPTHLPLHTYCFEKISEFKHRPCLINAATGESYTYGEVQETSR 103
           DD   EFIFRSKLP I IP HLPLH+YCFE IS+FK RPCLIN ATGE  TY +V  TSR
Sbjct: 7   DDHQEEFIFRSKLPDIYIPNHLPLHSYCFENISQFKDRPCLINGATGEIITYADVDLTSR 66

Query: 104 RVAAGLHKLGIGKGDVIMLLLQNTPQFVLAFLGTSYLGAAATTVNPFFMPAEIAKQAASS 163
           +VAAGL KLGI +GDVIMLLLQN+P+FV AFL  SY+GA  TT NPF+ PAE+AKQAA+S
Sbjct: 67  KVAAGLDKLGIKQGDVIMLLLQNSPEFVYAFLAASYIGAIITTANPFYTPAEVAKQAAAS 126

Query: 164 GAKLIITQAAFAEKVKKLSQENKATIKVIFIDDDPPLEGGYHFSSLIDDVAKVEMEDVKI 223
             KL+IT A + +KVK+ +   ++ +KV+ +D  PP     HFS L     + E+  VKI
Sbjct: 127 KTKLVITLAGYIDKVKEFT-GGESGVKVMCVDAPPPESECLHFSEL-TQADETEIPAVKI 186

Query: 224 SPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPHLYIRSDDVVLCVLPLFHI 283
            PDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDG+NP+LY   +DV+LCVLPLFHI
Sbjct: 187 HPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGDNPNLYFHQNDVILCVLPLFHI 246

Query: 284 YSLNTIMMCSLRVGAAILIVRKFDVNSLVELIPKYRVTFAPFVPPIVLAIVKSPAVDHSD 343
           YSLN+I++C LRVGAAILI++KF+++ L+ELI K++VT APFVPPIVL++ K P +   D
Sbjct: 247 YSLNSILLCGLRVGAAILIMQKFEISKLLELIEKFKVTIAPFVPPIVLSVAKCPDLHRYD 306

Query: 344 MSSLRMALSGAAPLGKNLEDAFRTKFSHVILGQGYGMTELGSVMTMSLAFAKERFEMKSG 403
           +SS+R  +SG AP+GK LEDA + K  H  LGQGYGMTE G V++M LAFAKE F +KSG
Sbjct: 307 LSSIRTVMSGGAPMGKELEDAVKEKLPHAKLGQGYGMTEAGPVLSMCLAFAKEPFPIKSG 366

Query: 404 GCGTIMRNTEMKIINPQTGASLPRNQAGEICIRSPQMMKGYLNDEEATKGIIDKDGWLHT 463
            CGT++RN EMKI++P TGASLPRNQ+GEICIR  Q+MKGY+ND EATKG ID+ GWLHT
Sbjct: 367 ACGTVVRNAEMKIVDPDTGASLPRNQSGEICIRGKQIMKGYINDAEATKGTIDEGGWLHT 426

Query: 464 GDIGFVDDDDEVFIVDRLKELIKYKGFQVAPAELEALLISHGHIADAAVIPMKDEVAGEV 523
           GDIGF+D+DDE+FIVDRLKELIKYKGFQVAPAELE++LISH +I DAAV+PMKDE AGEV
Sbjct: 427 GDIGFIDNDDELFIVDRLKELIKYKGFQVAPAELESMLISHPNITDAAVVPMKDEAAGEV 486

Query: 524 PVAFIVLSDGSNITEDEIKQYISKQFSGLISHYLLPIDGDFCIDFTVVFYKRINRVFFVD 583
           PVAF+V S+GS ITE++IKQYISKQ                     VVFYKRIN+ FF++
Sbjct: 487 PVAFVVRSNGSKITEEDIKQYISKQ---------------------VVFYKRINKAFFIE 544

Query: 584 SIPKSPSGKILRRQLRALLAT 605
            IPK+PSGKILR+ LRA L T
Sbjct: 547 EIPKNPSGKILRKILRAKLVT 544

BLAST of Lsi06G011330 vs. ExPASy Swiss-Prot
Match: P31684 (4-coumarate--CoA ligase 1 OS=Solanum tuberosum OX=4113 GN=4CL1 PE=3 SV=1)

HSP 1 Score: 732.3 bits (1889), Expect = 4.6e-210
Identity = 377/570 (66.14%), Postives = 442/570 (77.54%), Query Frame = 0

Query: 39  PMAAADDPSLEFIFRSKLPQIPIPTHLPLHTYCFEKISEFKHRPCLINAATGESYTYGEV 98
           PM      S + IFRSKLP I IP HLPLH+YCFE +SEF  RPCLI+ A    YTY EV
Sbjct: 2   PMDTETKQSGDLIFRSKLPDIYIPKHLPLHSYCFENLSEFNSRPCLIDGANDRIYTYAEV 61

Query: 99  QETSRRVAAGLHKLGIGKGDVIMLLLQNTPQFVLAFLGTSYLGAAATTVNPFFMPAEIAK 158
           + TSR+VA GL+KLGI + D IM+LL N P+FV AF+G SYLGA +T  NP F PAE+ K
Sbjct: 62  ELTSRKVAVGLNKLGIQQKDTIMILLPNCPEFVFAFIGASYLGAISTMANPLFTPAEVVK 121

Query: 159 QAASSGAKLIITQAAFAEKVKKLSQENKATIKVIFIDDDPPLEGGYHFSSLIDDVAKVEM 218
           QA +S AK++ITQA FA KVK  + EN   +KVI +D  P  EG  HFS LI    + E+
Sbjct: 122 QAKASSAKIVITQACFAGKVKDYAIEN--DLKVICVDSVP--EGCVHFSELIQS-DEHEI 181

Query: 219 EDVKISPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPHLYIRSDDVVLCVL 278
            DVKI PDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGEN +LY+ SDDV++CVL
Sbjct: 182 PDVKIQPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENANLYMHSDDVLMCVL 241

Query: 279 PLFHIYSLNTIMMCSLRVGAAILIVRKFDVNSLVELIPKYRVTFAPFVPPIVLAIVKSPA 338
           PLFHIYSLN++++C+LRVGAAILI++KFD+   +ELIPK++VT  PFVPPIVLAI KSP 
Sbjct: 242 PLFHIYSLNSVLLCALRVGAAILIMQKFDIAQFLELIPKHKVTIGPFVPPIVLAIAKSPL 301

Query: 339 VDHSDMSSLRMALSGAAPLGKNLEDAFRTKFSHVILGQGYGMTELGSVMTMSLAFAKERF 398
           VD+ D+SS+R  +SGAAPLGK LEDA R KF +  LGQGYGMTE G V+ M LAFAKE F
Sbjct: 302 VDNYDLSSVRTVMSGAAPLGKELEDAVRAKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPF 361

Query: 399 EMKSGGCGTIMRNTEMKIINPQTGASLPRNQAGEICIRSPQMMKGYLNDEEATKGIIDKD 458
           ++KSG CGT++RN EMKI++P TG SLPRNQ GEICIR  Q+MKGYLND EAT   I+K+
Sbjct: 362 DIKSGACGTVVRNAEMKIVDPDTGCSLPRNQPGEICIRGDQIMKGYLNDPEATARTIEKE 421

Query: 459 GWLHTGDIGFVDDDDEVFIVDRLKELIKYKGFQVAPAELEALLISHGHIADAAVIPMKDE 518
           GWLHTGDIGF+DDDDE+FIVDRLKELIKYKGFQVAPAELEALLI+H  I+DAAV+PM DE
Sbjct: 422 GWLHTGDIGFIDDDDELFIVDRLKELIKYKGFQVAPAELEALLINHPDISDAAVVPMIDE 481

Query: 519 VAGEVPVAFIVLSDGSNITEDEIKQYISKQFSGLISHYLLPIDGDFCIDFTVVFYKRINR 578
            AGEVPVAF+V S+GS ITEDE+K +ISKQ                     V+FYKRI R
Sbjct: 482 QAGEVPVAFVVRSNGSTITEDEVKDFISKQ---------------------VIFYKRIKR 541

Query: 579 VFFVDSIPKSPSGKILRRQLRALLATSIPN 609
           VFFV+++PKSPSGKILR+ LRA LA  I N
Sbjct: 542 VFFVETVPKSPSGKILRKDLRARLAAGISN 545

BLAST of Lsi06G011330 vs. ExPASy Swiss-Prot
Match: O24146 (4-coumarate--CoA ligase 2 OS=Nicotiana tabacum OX=4097 GN=4CL2 PE=1 SV=1)

HSP 1 Score: 731.5 bits (1887), Expect = 7.9e-210
Identity = 372/565 (65.84%), Postives = 442/565 (78.23%), Query Frame = 0

Query: 44  DDPSLEFIFRSKLPQIPIPTHLPLHTYCFEKISEFKHRPCLINAATGESYTYGEVQETSR 103
           D   ++ IFRSKLP I IP HLPLH+YCFE ISEF  RPCLIN A  + YTY +V+  SR
Sbjct: 4   DTKQVDIIFRSKLPDIYIPNHLPLHSYCFENISEFSSRPCLINGANKQIYTYADVELNSR 63

Query: 104 RVAAGLHKLGIGKGDVIMLLLQNTPQFVLAFLGTSYLGAAATTVNPFFMPAEIAKQAASS 163
           +VAAGLHK GI   D IM+LL N+P+FV AF+G SYLGA +T  NP F PAE+ KQA +S
Sbjct: 64  KVAAGLHKQGIQPKDTIMILLPNSPEFVFAFIGASYLGAISTMANPLFTPAEVVKQAKAS 123

Query: 164 GAKLIITQAAFAEKVKKLSQENKATIKVIFIDDDPPLEGGYHFSSLIDDVAKVEMEDVKI 223
            AK+I+TQA    KVK  + EN   +K+I ID  P  EG  HF S++    + ++ +V+I
Sbjct: 124 SAKIIVTQACHVNKVKDYAFEN--DVKIICIDSAP--EGCLHF-SVLTQANEHDIPEVEI 183

Query: 224 SPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPHLYIRSDDVVLCVLPLFHI 283
            PDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENP+LYI S+DV+LCVLPLFHI
Sbjct: 184 QPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYIHSEDVMLCVLPLFHI 243

Query: 284 YSLNTIMMCSLRVGAAILIVRKFDVNSLVELIPKYRVTFAPFVPPIVLAIVKSPAVDHSD 343
           YSLN++++C LRVGAAILI++KFD+ S +ELI +Y+VT  PFVPPIVLAI KSP VD  D
Sbjct: 244 YSLNSVLLCGLRVGAAILIMQKFDIVSFLELIQRYKVTIGPFVPPIVLAIAKSPMVDDYD 303

Query: 344 MSSLRMALSGAAPLGKNLEDAFRTKFSHVILGQGYGMTELGSVMTMSLAFAKERFEMKSG 403
           +SS+R  +SGAAPLGK LED  R KF +  LGQGYGMTE G V+ M LAFAKE FE+KSG
Sbjct: 304 LSSVRTVMSGAAPLGKELEDTVRAKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFEIKSG 363

Query: 404 GCGTIMRNTEMKIINPQTGASLPRNQAGEICIRSPQMMKGYLNDEEATKGIIDKDGWLHT 463
            CGT++RN EMKI++P+TG SLPRNQ+GEICIR  Q+MKGYLND EAT   IDK+GWL+T
Sbjct: 364 ACGTVVRNAEMKIVDPKTGNSLPRNQSGEICIRGDQIMKGYLNDPEATARTIDKEGWLYT 423

Query: 464 GDIGFVDDDDEVFIVDRLKELIKYKGFQVAPAELEALLISHGHIADAAVIPMKDEVAGEV 523
           GDIG++DDDDE+FIVDRLKELIKYKGFQVAPAELEALL++H +I+DAAV+PMKDE AGEV
Sbjct: 424 GDIGYIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLNHPNISDAAVVPMKDEQAGEV 483

Query: 524 PVAFIVLSDGSNITEDEIKQYISKQFSGLISHYLLPIDGDFCIDFTVVFYKRINRVFFVD 583
           PVAF+V S+GS ITEDE+K +ISKQ                     V+FYKRI RVFFVD
Sbjct: 484 PVAFVVRSNGSTITEDEVKDFISKQ---------------------VIFYKRIKRVFFVD 542

Query: 584 SIPKSPSGKILRRQLRALLATSIPN 609
           +IPKSPSGKILR+ LRA LA  +PN
Sbjct: 544 AIPKSPSGKILRKDLRAKLAAGLPN 542

BLAST of Lsi06G011330 vs. ExPASy Swiss-Prot
Match: O24145 (4-coumarate--CoA ligase 1 OS=Nicotiana tabacum OX=4097 GN=4CL1 PE=2 SV=1)

HSP 1 Score: 729.6 bits (1882), Expect = 3.0e-209
Identity = 371/560 (66.25%), Postives = 439/560 (78.39%), Query Frame = 0

Query: 49  EFIFRSKLPQIPIPTHLPLHTYCFEKISEFKHRPCLINAATGESYTYGEVQETSRRVAAG 108
           + IFRSKLP I IP HLPLH+YCFE ISEF  RPCLIN A  + YTY EV+ T R+VA G
Sbjct: 14  DLIFRSKLPDIYIPKHLPLHSYCFENISEFSSRPCLINGANDQIYTYAEVELTCRKVAVG 73

Query: 109 LHKLGIGKGDVIMLLLQNTPQFVLAFLGTSYLGAAATTVNPFFMPAEIAKQAASSGAKLI 168
           L+KLGI + D IM+LL N+P+FV AF+G SYLGA +T  NP F PAE+ KQA +S AK+I
Sbjct: 74  LNKLGIQQKDTIMILLPNSPEFVFAFMGASYLGAISTMANPLFTPAEVVKQAKASSAKII 133

Query: 169 ITQAAFAEKVKKLSQENKATIKVIFIDDDPPLEGGYHFSSLIDDVAKVEMEDVKISPDDV 228
           ITQ+ F  KVK  + EN   +KVI ID  P  EG  HFS L     + E+ +VKI PDDV
Sbjct: 134 ITQSCFVGKVKDYASEN--DVKVICIDSAP--EGCLHFSELTQS-DEHEIPEVKIQPDDV 193

Query: 229 VALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPHLYIRSDDVVLCVLPLFHIYSLNT 288
           VALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGEN +LY+ S+DV++CVLPLFHIYSLN+
Sbjct: 194 VALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENANLYMHSEDVLMCVLPLFHIYSLNS 253

Query: 289 IMMCSLRVGAAILIVRKFDVNSLVELIPKYRVTFAPFVPPIVLAIVKSPAVDHSDMSSLR 348
           I++C LRVGAAILI++KFD+   +ELI KY+V+  PFVPPIVLAI KSP VD  D+SS+R
Sbjct: 254 ILLCGLRVGAAILIMQKFDIAPFLELIQKYKVSIGPFVPPIVLAIAKSPIVDSYDLSSVR 313

Query: 349 MALSGAAPLGKNLEDAFRTKFSHVILGQGYGMTELGSVMTMSLAFAKERFEMKSGGCGTI 408
             +SGAAPLGK LEDA RTKF +  LGQGYGMTE G V+ M LAFAKE F++KSG CGT+
Sbjct: 314 TVMSGAAPLGKELEDAVRTKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFDIKSGACGTV 373

Query: 409 MRNTEMKIINPQTGASLPRNQAGEICIRSPQMMKGYLNDEEATKGIIDKDGWLHTGDIGF 468
           +RN EMKI++P TG SLPRNQ GEICIR  Q+MKGYLND EAT   IDK+GWLHTGDIGF
Sbjct: 374 VRNAEMKIVDPDTGCSLPRNQPGEICIRGDQIMKGYLNDPEATTRTIDKEGWLHTGDIGF 433

Query: 469 VDDDDEVFIVDRLKELIKYKGFQVAPAELEALLISHGHIADAAVIPMKDEVAGEVPVAFI 528
           +D+DDE+FIVDRLKELIKYKGFQVAPAE+EALL++H +I+DAAV+PMKDE AGEVPVAF+
Sbjct: 434 IDEDDELFIVDRLKELIKYKGFQVAPAEIEALLLNHPNISDAAVVPMKDEQAGEVPVAFV 493

Query: 529 VLSDGSNITEDEIKQYISKQFSGLISHYLLPIDGDFCIDFTVVFYKRINRVFFVDSIPKS 588
           V S+GS ITEDE+K +ISKQ                     V+FYKR+ RVFFV+++PKS
Sbjct: 494 VRSNGSAITEDEVKDFISKQ---------------------VIFYKRVKRVFFVETVPKS 547

Query: 589 PSGKILRRQLRALLATSIPN 609
           PSGKILR+ LRA LA  +PN
Sbjct: 554 PSGKILRKDLRARLAAGVPN 547

BLAST of Lsi06G011330 vs. ExPASy Swiss-Prot
Match: P31685 (4-coumarate--CoA ligase 2 OS=Solanum tuberosum OX=4113 GN=4CL2 PE=3 SV=1)

HSP 1 Score: 729.6 bits (1882), Expect = 3.0e-209
Identity = 376/570 (65.96%), Postives = 441/570 (77.37%), Query Frame = 0

Query: 39  PMAAADDPSLEFIFRSKLPQIPIPTHLPLHTYCFEKISEFKHRPCLINAATGESYTYGEV 98
           PM      S + IFRSKLP I IP HLPLH+YCFE +SEF  RPCLI+ A    YTY EV
Sbjct: 2   PMDIETKQSGDLIFRSKLPDIYIPKHLPLHSYCFENLSEFNSRPCLIDGANDRIYTYAEV 61

Query: 99  QETSRRVAAGLHKLGIGKGDVIMLLLQNTPQFVLAFLGTSYLGAAATTVNPFFMPAEIAK 158
           + TSR+VA GL+KLGI + D IM+LL N P+FV AF+G SYLGA +T  NP F PAE+ K
Sbjct: 62  ELTSRKVAVGLNKLGIQQKDTIMILLPNCPEFVFAFIGASYLGAISTMANPLFTPAEVVK 121

Query: 159 QAASSGAKLIITQAAFAEKVKKLSQENKATIKVIFIDDDPPLEGGYHFSSLIDDVAKVEM 218
           QA +S AK++ITQA FA KVK  + EN   +KVI +D  P  EG  HFS LI    + E+
Sbjct: 122 QAKASSAKIVITQACFAGKVKDYAIEN--DLKVICVDSAP--EGCVHFSELIQS-DEHEI 181

Query: 219 EDVKISPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPHLYIRSDDVVLCVL 278
            DVKI PDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGEN +LY+ SDDV++CVL
Sbjct: 182 PDVKIQPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENANLYMHSDDVLMCVL 241

Query: 279 PLFHIYSLNTIMMCSLRVGAAILIVRKFDVNSLVELIPKYRVTFAPFVPPIVLAIVKSPA 338
           PLFHIYSLN++++C+LRVGAAILI++KFD+   +ELIPK++VT  PFVPPIVLAI KSP 
Sbjct: 242 PLFHIYSLNSVLLCALRVGAAILIMQKFDIAQFLELIPKHKVTIGPFVPPIVLAIAKSPL 301

Query: 339 VDHSDMSSLRMALSGAAPLGKNLEDAFRTKFSHVILGQGYGMTELGSVMTMSLAFAKERF 398
           V + D+SS+R  +SGAAPLGK LEDA R KF +  LGQGYGMTE G V+ M LAFAKE F
Sbjct: 302 VHNYDLSSVRTVMSGAAPLGKELEDAVRAKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPF 361

Query: 399 EMKSGGCGTIMRNTEMKIINPQTGASLPRNQAGEICIRSPQMMKGYLNDEEATKGIIDKD 458
           ++KSG CGT++RN EMKI++P TG SLPRNQ GEICIR  Q+MKGYLND EAT   I+K+
Sbjct: 362 DIKSGACGTVVRNAEMKIVDPDTGCSLPRNQPGEICIRGDQIMKGYLNDPEATARTIEKE 421

Query: 459 GWLHTGDIGFVDDDDEVFIVDRLKELIKYKGFQVAPAELEALLISHGHIADAAVIPMKDE 518
           GWLHTGDIGF+DDDDE+FIVDRLKELIKYKGFQVAPAELEALLI+H  I+DAAV+PM DE
Sbjct: 422 GWLHTGDIGFIDDDDELFIVDRLKELIKYKGFQVAPAELEALLINHPDISDAAVVPMIDE 481

Query: 519 VAGEVPVAFIVLSDGSNITEDEIKQYISKQFSGLISHYLLPIDGDFCIDFTVVFYKRINR 578
            AGEVPVAF+V S+GS ITEDE+K +ISKQ                     V+FYKRI R
Sbjct: 482 QAGEVPVAFVVRSNGSTITEDEVKDFISKQ---------------------VIFYKRIKR 541

Query: 579 VFFVDSIPKSPSGKILRRQLRALLATSIPN 609
           VFFV+++PKSPSGKILR+ LRA LA  I N
Sbjct: 542 VFFVETVPKSPSGKILRKDLRARLAAGISN 545

BLAST of Lsi06G011330 vs. ExPASy TrEMBL
Match: A0A5D3CN41 (4-coumarate--CoA ligase 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold152G00670 PE=4 SV=1)

HSP 1 Score: 927.5 bits (2396), Expect = 2.8e-266
Identity = 483/571 (84.59%), Postives = 506/571 (88.62%), Query Frame = 0

Query: 40  MAAADDPSLEFIFRSKLPQIPIPTHLPLHTYCFEKISEFKHRPCLINAATGESYTYGEVQ 99
           MAAAD  S EF+FRSKLP+IPI THLPLHTYCFE ISEFK RPCLINAATG +YTYGEVQ
Sbjct: 1   MAAADGSSPEFLFRSKLPEIPISTHLPLHTYCFENISEFKQRPCLINAATGHTYTYGEVQ 60

Query: 100 ETSRRVAAGLHKLGIGKGDVIMLLLQNTPQFVLAFLGTSYLGAAATTVNPFFMPAEIAKQ 159
            TSRRVAAGLHKLGIGKGDVIMLLLQNTP+FVLAFL  SYLGAAAT  NPFFMPAEIAKQ
Sbjct: 61  ATSRRVAAGLHKLGIGKGDVIMLLLQNTPEFVLAFLAASYLGAAATMANPFFMPAEIAKQ 120

Query: 160 AASSGAKLIITQAAFAEKVKKLSQENKATIKVIFI-DDDPPLEGGYHFSSLIDDVAK--- 219
           A SSGAK+IITQ+AFAEKVKKLSQ ++  IKVIFI DDDPP +GGY FSSLIDDVAK   
Sbjct: 121 AVSSGAKVIITQSAFAEKVKKLSQGSEMIIKVIFIDDDDPPTKGGYKFSSLIDDVAKEEE 180

Query: 220 VEMEDVKISPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPHLYIRSDDVVL 279
           +EM DVKISPDDVVALPYSSGTTGLPKGVMLTHKG VTSVAQQVDGENPHLYIRSDDVVL
Sbjct: 181 LEMGDVKISPDDVVALPYSSGTTGLPKGVMLTHKGSVTSVAQQVDGENPHLYIRSDDVVL 240

Query: 280 CVLPLFHIYSLNTIMMCSLRVGAAILIVRKFDVNSLVELIPKYRVTFAPFVPPIVLAIVK 339
           C+LPLFHIYSLN+IMMCSLRVGAAILIV+KFD+NSL+EL+PKY+VTFAP VPPIVLAI K
Sbjct: 241 CLLPLFHIYSLNSIMMCSLRVGAAILIVQKFDINSLLELVPKYKVTFAPVVPPIVLAIAK 300

Query: 340 SPAVDHSDMSSLRMALSGAAPLGKNLEDAFRTKFSHVILGQGYGMTELGSVMTMSLAFAK 399
           SPAVDH DMSSLRM LSGAAPLGKNLEDAFR K  HVILGQGYGMTE GSVMTMSLAFAK
Sbjct: 301 SPAVDHFDMSSLRMVLSGAAPLGKNLEDAFRAKLPHVILGQGYGMTEAGSVMTMSLAFAK 360

Query: 400 ERFEMKSGGCGTIMRNTEMKIINPQTGASLPRNQAGEICIRSPQMMKGYLNDEEATKGII 459
           E F +KSGGCGTIMRN+EMKI+ PQTG SLPRN+ GEICIRS QMMKGYLNDEEATK II
Sbjct: 361 EGFGIKSGGCGTIMRNSEMKIVCPQTGVSLPRNRTGEICIRSFQMMKGYLNDEEATKAII 420

Query: 460 DKDGWLHTGDIGFVDDDDEVFIVDRLKELIKYKGFQVAPAELEALLISHGHIADAAVIPM 519
           DKDGWLHTGDIG+VDDDDE+FIVDRLKELIKYKGFQVAPAELEALLISHGHIADAAVIPM
Sbjct: 421 DKDGWLHTGDIGYVDDDDELFIVDRLKELIKYKGFQVAPAELEALLISHGHIADAAVIPM 480

Query: 520 KDEVAGEVPVAFIVLSDGSNITEDEIKQYISKQFSGLISHYLLPIDGDFCIDFTVVFYKR 579
            DEVAGEVPV FIV  DGSNITEDEIKQYISKQ                     VVFYKR
Sbjct: 481 NDEVAGEVPVTFIVRFDGSNITEDEIKQYISKQ---------------------VVFYKR 540

Query: 580 INRVFFVDSIPKSPSGKILRRQLRALLATSI 607
           INRVFFVDSIPKSPSGKILRRQLRALLA SI
Sbjct: 541 INRVFFVDSIPKSPSGKILRRQLRALLAASI 550

BLAST of Lsi06G011330 vs. ExPASy TrEMBL
Match: A0A1S3BWP0 (4-coumarate--CoA ligase 1-like OS=Cucumis melo OX=3656 GN=LOC103494274 PE=4 SV=1)

HSP 1 Score: 927.5 bits (2396), Expect = 2.8e-266
Identity = 483/571 (84.59%), Postives = 506/571 (88.62%), Query Frame = 0

Query: 40  MAAADDPSLEFIFRSKLPQIPIPTHLPLHTYCFEKISEFKHRPCLINAATGESYTYGEVQ 99
           MAAAD  S EF+FRSKLP+IPI THLPLHTYCFE ISEFK RPCLINAATG +YTYGEVQ
Sbjct: 1   MAAADGSSPEFLFRSKLPEIPISTHLPLHTYCFENISEFKQRPCLINAATGHTYTYGEVQ 60

Query: 100 ETSRRVAAGLHKLGIGKGDVIMLLLQNTPQFVLAFLGTSYLGAAATTVNPFFMPAEIAKQ 159
            TSRRVAAGLHKLGIGKGDVIMLLLQNTP+FVLAFL  SYLGAAAT  NPFFMPAEIAKQ
Sbjct: 61  ATSRRVAAGLHKLGIGKGDVIMLLLQNTPEFVLAFLAASYLGAAATMANPFFMPAEIAKQ 120

Query: 160 AASSGAKLIITQAAFAEKVKKLSQENKATIKVIFI-DDDPPLEGGYHFSSLIDDVAK--- 219
           A SSGAK+IITQ+AFAEKVKKLSQ ++  IKVIFI DDDPP +GGY FSSLIDDVAK   
Sbjct: 121 AVSSGAKVIITQSAFAEKVKKLSQGSEMIIKVIFIDDDDPPTKGGYKFSSLIDDVAKEEE 180

Query: 220 VEMEDVKISPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPHLYIRSDDVVL 279
           +EM DVKISPDDVVALPYSSGTTGLPKGVMLTHKG VTSVAQQVDGENPHLYIRSDDVVL
Sbjct: 181 LEMGDVKISPDDVVALPYSSGTTGLPKGVMLTHKGSVTSVAQQVDGENPHLYIRSDDVVL 240

Query: 280 CVLPLFHIYSLNTIMMCSLRVGAAILIVRKFDVNSLVELIPKYRVTFAPFVPPIVLAIVK 339
           C+LPLFHIYSLN+IMMCSLRVGAAILIV+KFD+NSL+EL+PKY+VTFAP VPPIVLAI K
Sbjct: 241 CLLPLFHIYSLNSIMMCSLRVGAAILIVQKFDINSLLELVPKYKVTFAPVVPPIVLAIAK 300

Query: 340 SPAVDHSDMSSLRMALSGAAPLGKNLEDAFRTKFSHVILGQGYGMTELGSVMTMSLAFAK 399
           SPAVDH DMSSLRM LSGAAPLGKNLEDAFR K  HVILGQGYGMTE GSVMTMSLAFAK
Sbjct: 301 SPAVDHFDMSSLRMVLSGAAPLGKNLEDAFRAKLPHVILGQGYGMTEAGSVMTMSLAFAK 360

Query: 400 ERFEMKSGGCGTIMRNTEMKIINPQTGASLPRNQAGEICIRSPQMMKGYLNDEEATKGII 459
           E F +KSGGCGTIMRN+EMKI+ PQTG SLPRN+ GEICIRS QMMKGYLNDEEATK II
Sbjct: 361 EGFGIKSGGCGTIMRNSEMKIVCPQTGVSLPRNRTGEICIRSFQMMKGYLNDEEATKAII 420

Query: 460 DKDGWLHTGDIGFVDDDDEVFIVDRLKELIKYKGFQVAPAELEALLISHGHIADAAVIPM 519
           DKDGWLHTGDIG+VDDDDE+FIVDRLKELIKYKGFQVAPAELEALLISHGHIADAAVIPM
Sbjct: 421 DKDGWLHTGDIGYVDDDDELFIVDRLKELIKYKGFQVAPAELEALLISHGHIADAAVIPM 480

Query: 520 KDEVAGEVPVAFIVLSDGSNITEDEIKQYISKQFSGLISHYLLPIDGDFCIDFTVVFYKR 579
            DEVAGEVPV FIV  DGSNITEDEIKQYISKQ                     VVFYKR
Sbjct: 481 NDEVAGEVPVTFIVRFDGSNITEDEIKQYISKQ---------------------VVFYKR 540

Query: 580 INRVFFVDSIPKSPSGKILRRQLRALLATSI 607
           INRVFFVDSIPKSPSGKILRRQLRALLA SI
Sbjct: 541 INRVFFVDSIPKSPSGKILRRQLRALLAASI 550

BLAST of Lsi06G011330 vs. ExPASy TrEMBL
Match: A0A5A7UT30 (4-coumarate--CoA ligase 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold274G006230 PE=4 SV=1)

HSP 1 Score: 926.8 bits (2394), Expect = 4.7e-266
Identity = 483/573 (84.29%), Postives = 506/573 (88.31%), Query Frame = 0

Query: 40  MAAADDPSLEFIFRSKLPQIPIPTHLPLHTYCFEKISEFKHRPCLINAATGESYTYGEVQ 99
           MAAAD  S EF+FRSKLP+IPI THLPLHTYCFE ISEFK RPCLINAATG +YTYGEVQ
Sbjct: 1   MAAADGSSPEFLFRSKLPEIPISTHLPLHTYCFENISEFKQRPCLINAATGHTYTYGEVQ 60

Query: 100 ETSRRVAAGLHKLGIGKGDVIMLLLQNTPQFVLAFLGTSYLGAAATTVNPFFMPAEIAKQ 159
             SRRVAAGLHKLGIGKGDVIMLLLQNTP+FVLAFL  SYLGAAAT  NPFFMPAEIAKQ
Sbjct: 61  AISRRVAAGLHKLGIGKGDVIMLLLQNTPEFVLAFLAASYLGAAATMANPFFMPAEIAKQ 120

Query: 160 AASSGAKLIITQAAFAEKVKKLSQENKATIKVIFI-DDDPPLEGGYHFSSLIDDVAK--- 219
           A SSGAK+IITQ+AFAEKVKKLSQ ++  IKVIFI DDDPP +GGY FSSLIDDVAK   
Sbjct: 121 AVSSGAKVIITQSAFAEKVKKLSQGSEMIIKVIFIDDDDPPTKGGYKFSSLIDDVAKEEE 180

Query: 220 VEMEDVKISPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPHLYIRSDDVVL 279
           +EM DVKISPDDVVALPYSSGTTGLPKGVMLTHKG VTSVAQQVDGENPHLYIRSDDVVL
Sbjct: 181 LEMGDVKISPDDVVALPYSSGTTGLPKGVMLTHKGSVTSVAQQVDGENPHLYIRSDDVVL 240

Query: 280 CVLPLFHIYSLNTIMMCSLRVGAAILIVRKFDVNSLVELIPKYRVTFAPFVPPIVLAIVK 339
           C+LPLFHIYSLN+IMMCSLRVGAAILIV+KFD+NSL+EL+PKY+VTFAP VPPIVLAI K
Sbjct: 241 CLLPLFHIYSLNSIMMCSLRVGAAILIVQKFDINSLLELVPKYKVTFAPVVPPIVLAIAK 300

Query: 340 SPAVDHSDMSSLRMALSGAAPLGKNLEDAFRTKFSHVILGQGYGMTELGSVMTMSLAFAK 399
           SPAVDH DMSSLRM LSGAAPLGKNLEDAFR K  HVILGQGYGMTE GSVMTMSLAFAK
Sbjct: 301 SPAVDHFDMSSLRMVLSGAAPLGKNLEDAFRAKLPHVILGQGYGMTEAGSVMTMSLAFAK 360

Query: 400 ERFEMKSGGCGTIMRNTEMKIINPQTGASLPRNQAGEICIRSPQMMKGYLNDEEATKGII 459
           E F +KSGGCGTIMRN+EMKI+ PQTG SLPRN+ GEICIRS QMMKGYLNDEEATK II
Sbjct: 361 EGFGIKSGGCGTIMRNSEMKIVCPQTGVSLPRNRTGEICIRSFQMMKGYLNDEEATKAII 420

Query: 460 DKDGWLHTGDIGFVDDDDEVFIVDRLKELIKYKGFQVAPAELEALLISHGHIADAAVIPM 519
           DKDGWLHTGDIG+VDDDDE+FIVDRLKELIKYKGFQVAPAELEALLISHGHIADAAVIPM
Sbjct: 421 DKDGWLHTGDIGYVDDDDELFIVDRLKELIKYKGFQVAPAELEALLISHGHIADAAVIPM 480

Query: 520 KDEVAGEVPVAFIVLSDGSNITEDEIKQYISKQFSGLISHYLLPIDGDFCIDFTVVFYKR 579
            DEVAGEVPV FIV  DGSNITEDEIKQYISKQ                     VVFYKR
Sbjct: 481 NDEVAGEVPVTFIVRFDGSNITEDEIKQYISKQ---------------------VVFYKR 540

Query: 580 INRVFFVDSIPKSPSGKILRRQLRALLATSIPN 609
           INRVFFVDSIPKSPSGKILRRQLRALLA SI N
Sbjct: 541 INRVFFVDSIPKSPSGKILRRQLRALLAASILN 552

BLAST of Lsi06G011330 vs. ExPASy TrEMBL
Match: A0A0A0LWT6 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G423020 PE=4 SV=1)

HSP 1 Score: 909.4 bits (2349), Expect = 7.8e-261
Identity = 478/571 (83.71%), Postives = 502/571 (87.92%), Query Frame = 0

Query: 40  MAAADDPSLEFIFRSKLPQIPIPTHLPLHTYCFEKISEFKHRPCLINAATGESYTYGEVQ 99
           MAAAD  S EFIFRSKLP+IPI THLPLHTYCFE ISEFKHRPCLINAATG  YTYGEVQ
Sbjct: 1   MAAADASSPEFIFRSKLPEIPISTHLPLHTYCFENISEFKHRPCLINAATGHIYTYGEVQ 60

Query: 100 ETSRRVAAGLHKLGIGKGDVIMLLLQNTPQFVLAFLGTSYLGAAATTVNPFFMPAEIAKQ 159
            TSRRVAAGLHKLGIGKGDVIMLLLQNTP+FVLAFLG SYLGAAAT  NPFFM +EIAKQ
Sbjct: 61  ATSRRVAAGLHKLGIGKGDVIMLLLQNTPEFVLAFLGASYLGAAATMANPFFMQSEIAKQ 120

Query: 160 AASSGAKLIITQAAFAEKVKKLSQENKATIKVIFIDD-DPPLEGGYHFSSLIDDVAK--- 219
           A SSGAK+IITQAAFAEKVK LSQEN+  IKVIFIDD DPP      FSSLI+DVAK   
Sbjct: 121 AVSSGAKVIITQAAFAEKVKNLSQENEMIIKVIFIDDNDPP-----QFSSLIEDVAKEEE 180

Query: 220 VEMEDVKISPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPHLYIRSDDVVL 279
           +EM DVKISP+DVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPHL+IRSDDVVL
Sbjct: 181 LEMGDVKISPEDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPHLHIRSDDVVL 240

Query: 280 CVLPLFHIYSLNTIMMCSLRVGAAILIVRKFDVNSLVELIPKYRVTFAPFVPPIVLAIVK 339
           C+LPLFHIYSLN+IMMC+LRVGAAIL+V+KFDVNSLVEL+ KY+VTFAPFVPPIVLAI K
Sbjct: 241 CLLPLFHIYSLNSIMMCALRVGAAILLVQKFDVNSLVELVSKYKVTFAPFVPPIVLAIAK 300

Query: 340 SPAVDHSDMSSLRMALSGAAPLGKNLEDAFRTKFSHVILGQGYGMTELGSVMTMSLAFAK 399
           SPAVDH DMSSLR+ LSGAAPLGKNLEDAFR K  HVILGQGYGMTE GSVMTMSLAF K
Sbjct: 301 SPAVDHFDMSSLRIVLSGAAPLGKNLEDAFRAKLPHVILGQGYGMTEAGSVMTMSLAFVK 360

Query: 400 ERFEMKSGGCGTIMRNTEMKIINPQTGASLPRNQAGEICIRSPQMMKGYLNDEEATKGII 459
           E F +KSGGCGTIMRN+EMKI+N QTG SLPRNQ GEICIRS QMMKGYLNDE+ATK II
Sbjct: 361 EGFGIKSGGCGTIMRNSEMKILNLQTGESLPRNQTGEICIRSSQMMKGYLNDEDATKAII 420

Query: 460 DKDGWLHTGDIGFVDDDDEVFIVDRLKELIKYKGFQVAPAELEALLISHGHIADAAVIPM 519
           D+DGWLHTGDIGFVDDDDE+FIVDRLKELIKYKGFQVAPAELEALLISH HIADAAVIPM
Sbjct: 421 DEDGWLHTGDIGFVDDDDELFIVDRLKELIKYKGFQVAPAELEALLISHAHIADAAVIPM 480

Query: 520 KDEVAGEVPVAFIVLSDGSNITEDEIKQYISKQFSGLISHYLLPIDGDFCIDFTVVFYKR 579
            DEVAGEVPVAFIV  DGSNITEDEIKQ+ISKQ                     VVFYKR
Sbjct: 481 NDEVAGEVPVAFIVRFDGSNITEDEIKQFISKQ---------------------VVFYKR 540

Query: 580 INRVFFVDSIPKSPSGKILRRQLRALLATSI 607
           INRVFFVDSIPKSPSGKILRRQLRALLA SI
Sbjct: 541 INRVFFVDSIPKSPSGKILRRQLRALLAASI 545

BLAST of Lsi06G011330 vs. ExPASy TrEMBL
Match: A0A6J1JA64 (4-coumarate--CoA ligase 1-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111484944 PE=4 SV=1)

HSP 1 Score: 873.6 bits (2256), Expect = 4.7e-250
Identity = 452/567 (79.72%), Postives = 482/567 (85.01%), Query Frame = 0

Query: 42  AADDPSLEFIFRSKLPQIPIPTHLPLHTYCFEKISEFKHRPCLINAATGESYTYGEVQET 101
           AA DPS EFIFRSKLP + IP HLPLHTYCFE+ISEFKH PCLINAATG+ YTYGEV  T
Sbjct: 2   AAADPSPEFIFRSKLPDLTIPNHLPLHTYCFERISEFKHNPCLINAATGDIYTYGEVHVT 61

Query: 102 SRRVAAGLHKLGIGKGDVIMLLLQNTPQFVLAFLGTSYLGAAATTVNPFFMPAEIAKQAA 161
           +RRVAAGLHKLGIGKGDVIMLLLQNTPQF  AFLG S+  A AT  NPFF PAEIAKQAA
Sbjct: 62  ARRVAAGLHKLGIGKGDVIMLLLQNTPQFAFAFLGASFACAIATMANPFFTPAEIAKQAA 121

Query: 162 SSGAKLIITQAAFAEKVKKLSQENKATIKVIFIDDDPPLEGGYHFSSLIDDVAKVEMEDV 221
           SSGAKLIITQAAFAEKVK L QEN+  +KVIFID  P  EGG HFSSL D+  + E+ DV
Sbjct: 122 SSGAKLIITQAAFAEKVKNLPQENQGILKVIFIDSPP--EGGCHFSSLTDEAKEEELTDV 181

Query: 222 KISPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPHLYIRSDDVVLCVLPLF 281
           K SPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPH +IRS+DVVLCVLPLF
Sbjct: 182 KFSPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPHFHIRSNDVVLCVLPLF 241

Query: 282 HIYSLNTIMMCSLRVGAAILIVRKFDVNSLVELIPKYRVTFAPFVPPIVLAIVKSPAVDH 341
           HIYSL++I+M SLRVGAAIL+V+KFD+NSLV L+PKYRVT A  VPPIVLA+VKSP VD 
Sbjct: 242 HIYSLSSIVMSSLRVGAAILLVQKFDINSLVALVPKYRVTIATVVPPIVLAMVKSPVVDC 301

Query: 342 SDMSSLRMALSGAAPLGKNLEDAFRTKFSHVILGQGYGMTELGSVMTMSLAFAKERFEMK 401
           SDMSS+R+ LSGAAPLGKNLEDAFR KF H ILGQGYGMTE GSV+TMSLA AKERF +K
Sbjct: 302 SDMSSIRIVLSGAAPLGKNLEDAFRAKFPHAILGQGYGMTESGSVLTMSLALAKERFAIK 361

Query: 402 SGGCGTIMRNTEMKIINPQTGASLPRNQAGEICIRSPQMMKGYLNDEEATKGIIDKDGWL 461
           SG CGT+MRN EMKI+NP+TG SL RNQ GEI +RSPQMMKGYLNDEEATKGIID DGWL
Sbjct: 362 SGACGTLMRNAEMKIVNPETGVSLSRNQPGEIYVRSPQMMKGYLNDEEATKGIIDGDGWL 421

Query: 462 HTGDIGFVDDDDEVFIVDRLKELIKYKGFQVAPAELEALLISHGHIADAAVIPMKDEVAG 521
           HTGDIG VDDDDEVFIVDRLKELIKYKGFQVAPAELE LLIS+ HI D AVIPMKDEVAG
Sbjct: 422 HTGDIGLVDDDDEVFIVDRLKELIKYKGFQVAPAELEGLLISNVHIVDVAVIPMKDEVAG 481

Query: 522 EVPVAFIVLSDGSNITEDEIKQYISKQFSGLISHYLLPIDGDFCIDFTVVFYKRINRVFF 581
           E PVAFIV SDGSNITEDEIK+YISKQ                     VVFYKRINRVFF
Sbjct: 482 EAPVAFIVRSDGSNITEDEIKEYISKQ---------------------VVFYKRINRVFF 541

Query: 582 VDSIPKSPSGKILRRQLRALLATSIPN 609
           VDSIPKSPSGKILRRQLRALL  SIPN
Sbjct: 542 VDSIPKSPSGKILRRQLRALLPASIPN 545

BLAST of Lsi06G011330 vs. NCBI nr
Match: XP_038878634.1 (4-coumarate--CoA ligase 1-like [Benincasa hispida])

HSP 1 Score: 937.2 bits (2421), Expect = 7.2e-269
Identity = 488/576 (84.72%), Postives = 509/576 (88.37%), Query Frame = 0

Query: 40  MAAADDPSLEFIFRSKLPQIPIPTHLPLHTYCFEKISEFKHRPCLINAATGESYTYGEVQ 99
           MAA +D S EFIFRSKLP+I IPTHLPLHTY FEK+SEF +RPCLINA TGE++TYGEV 
Sbjct: 1   MAATEDSSPEFIFRSKLPEISIPTHLPLHTYLFEKVSEFTNRPCLINATTGETFTYGEVH 60

Query: 100 ETSRRVAAGLHKLGIGKGDVIMLLLQNTPQFVLAFLGTSYLGAAATTVNPFFMPAEIAKQ 159
           ETSRRVAAGLHKLGI KGDVIMLLLQNTPQFV AFLG SYLGAAATT NPFF PAEI KQ
Sbjct: 61  ETSRRVAAGLHKLGIRKGDVIMLLLQNTPQFVFAFLGASYLGAAATTANPFFTPAEITKQ 120

Query: 160 AASSGAKLIITQAAFAEKVKKLSQENKATIKVIFI----DDDPPLEGGYHFSSLIDDVAK 219
           AASS  KLIITQAAFAEKVKKLSQENKA IKVIFI    DDDPP +GGYHFSSL DDVAK
Sbjct: 121 AASSSIKLIITQAAFAEKVKKLSQENKAIIKVIFIDDDDDDDPPPDGGYHFSSLTDDVAK 180

Query: 220 ---VEMEDVKISPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPHLYIRSDD 279
              +EM D K+SPDDVVALPYSSGTTGLPKGVMLTHKGLV  VAQQVDGENPH  I+SDD
Sbjct: 181 EEEMEMGDAKVSPDDVVALPYSSGTTGLPKGVMLTHKGLVAIVAQQVDGENPHFNIQSDD 240

Query: 280 VVLCVLPLFHIYSLNTIMMCSLRVGAAILIVRKFDVNSLVELIPKYRVTFAPFVPPIVLA 339
           VVLCVLPLFHIYSLN+IMMCSLRVGAAILIV+KFDVN L+ LIPKY+VT AP VPPIVLA
Sbjct: 241 VVLCVLPLFHIYSLNSIMMCSLRVGAAILIVQKFDVNLLLGLIPKYKVTIAPIVPPIVLA 300

Query: 340 IVKSPAVDHSDMSSLRMALSGAAPLGKNLEDAFRTKFSHVILGQGYGMTELGSVMTMSLA 399
           IVKSPAVDHSDMSSLR+ +SGAAPLGKNLEDAFRTK  HVILGQGYGMTE GS MTMSLA
Sbjct: 301 IVKSPAVDHSDMSSLRIVMSGAAPLGKNLEDAFRTKLPHVILGQGYGMTESGSAMTMSLA 360

Query: 400 FAKERFEMKSGGCGTIMRNTEMKIINPQTGASLPRNQAGEICIRSPQMMKGYLNDEEATK 459
           FAKE FE+KSGGCGTIMRNTEMKIINPQTGASLPRNQAGEICI+S Q+MKGYLNDEEATK
Sbjct: 361 FAKEGFEIKSGGCGTIMRNTEMKIINPQTGASLPRNQAGEICIKSLQIMKGYLNDEEATK 420

Query: 460 GIIDKDGWLHTGDIGFVDDDDEVFIVDRLKELIKYKGFQVAPAELEALLISHGHIADAAV 519
           GIIDKDGWLHTGD+GFVDDDDEVFIVDRLKELIKYKGFQVAPAELEALLISHGHIADAAV
Sbjct: 421 GIIDKDGWLHTGDVGFVDDDDEVFIVDRLKELIKYKGFQVAPAELEALLISHGHIADAAV 480

Query: 520 IPMKDEVAGEVPVAFIVLSDGSNITEDEIKQYISKQFSGLISHYLLPIDGDFCIDFTVVF 579
           IPMKDEVAGEVPVAFI  SDG+NITEDEIKQYISKQ                     VVF
Sbjct: 481 IPMKDEVAGEVPVAFIARSDGTNITEDEIKQYISKQ---------------------VVF 540

Query: 580 YKRINRVFFVDSIPKSPSGKILRRQLRALLATSIPN 609
           YKRINRVFFVDSIPKSPSGKILRRQLRALLA SIPN
Sbjct: 541 YKRINRVFFVDSIPKSPSGKILRRQLRALLAASIPN 555

BLAST of Lsi06G011330 vs. NCBI nr
Match: XP_008453615.1 (PREDICTED: 4-coumarate--CoA ligase 1-like [Cucumis melo] >TYK11846.1 4-coumarate--CoA ligase 1-like [Cucumis melo var. makuwa])

HSP 1 Score: 927.5 bits (2396), Expect = 5.7e-266
Identity = 483/571 (84.59%), Postives = 506/571 (88.62%), Query Frame = 0

Query: 40  MAAADDPSLEFIFRSKLPQIPIPTHLPLHTYCFEKISEFKHRPCLINAATGESYTYGEVQ 99
           MAAAD  S EF+FRSKLP+IPI THLPLHTYCFE ISEFK RPCLINAATG +YTYGEVQ
Sbjct: 1   MAAADGSSPEFLFRSKLPEIPISTHLPLHTYCFENISEFKQRPCLINAATGHTYTYGEVQ 60

Query: 100 ETSRRVAAGLHKLGIGKGDVIMLLLQNTPQFVLAFLGTSYLGAAATTVNPFFMPAEIAKQ 159
            TSRRVAAGLHKLGIGKGDVIMLLLQNTP+FVLAFL  SYLGAAAT  NPFFMPAEIAKQ
Sbjct: 61  ATSRRVAAGLHKLGIGKGDVIMLLLQNTPEFVLAFLAASYLGAAATMANPFFMPAEIAKQ 120

Query: 160 AASSGAKLIITQAAFAEKVKKLSQENKATIKVIFI-DDDPPLEGGYHFSSLIDDVAK--- 219
           A SSGAK+IITQ+AFAEKVKKLSQ ++  IKVIFI DDDPP +GGY FSSLIDDVAK   
Sbjct: 121 AVSSGAKVIITQSAFAEKVKKLSQGSEMIIKVIFIDDDDPPTKGGYKFSSLIDDVAKEEE 180

Query: 220 VEMEDVKISPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPHLYIRSDDVVL 279
           +EM DVKISPDDVVALPYSSGTTGLPKGVMLTHKG VTSVAQQVDGENPHLYIRSDDVVL
Sbjct: 181 LEMGDVKISPDDVVALPYSSGTTGLPKGVMLTHKGSVTSVAQQVDGENPHLYIRSDDVVL 240

Query: 280 CVLPLFHIYSLNTIMMCSLRVGAAILIVRKFDVNSLVELIPKYRVTFAPFVPPIVLAIVK 339
           C+LPLFHIYSLN+IMMCSLRVGAAILIV+KFD+NSL+EL+PKY+VTFAP VPPIVLAI K
Sbjct: 241 CLLPLFHIYSLNSIMMCSLRVGAAILIVQKFDINSLLELVPKYKVTFAPVVPPIVLAIAK 300

Query: 340 SPAVDHSDMSSLRMALSGAAPLGKNLEDAFRTKFSHVILGQGYGMTELGSVMTMSLAFAK 399
           SPAVDH DMSSLRM LSGAAPLGKNLEDAFR K  HVILGQGYGMTE GSVMTMSLAFAK
Sbjct: 301 SPAVDHFDMSSLRMVLSGAAPLGKNLEDAFRAKLPHVILGQGYGMTEAGSVMTMSLAFAK 360

Query: 400 ERFEMKSGGCGTIMRNTEMKIINPQTGASLPRNQAGEICIRSPQMMKGYLNDEEATKGII 459
           E F +KSGGCGTIMRN+EMKI+ PQTG SLPRN+ GEICIRS QMMKGYLNDEEATK II
Sbjct: 361 EGFGIKSGGCGTIMRNSEMKIVCPQTGVSLPRNRTGEICIRSFQMMKGYLNDEEATKAII 420

Query: 460 DKDGWLHTGDIGFVDDDDEVFIVDRLKELIKYKGFQVAPAELEALLISHGHIADAAVIPM 519
           DKDGWLHTGDIG+VDDDDE+FIVDRLKELIKYKGFQVAPAELEALLISHGHIADAAVIPM
Sbjct: 421 DKDGWLHTGDIGYVDDDDELFIVDRLKELIKYKGFQVAPAELEALLISHGHIADAAVIPM 480

Query: 520 KDEVAGEVPVAFIVLSDGSNITEDEIKQYISKQFSGLISHYLLPIDGDFCIDFTVVFYKR 579
            DEVAGEVPV FIV  DGSNITEDEIKQYISKQ                     VVFYKR
Sbjct: 481 NDEVAGEVPVTFIVRFDGSNITEDEIKQYISKQ---------------------VVFYKR 540

Query: 580 INRVFFVDSIPKSPSGKILRRQLRALLATSI 607
           INRVFFVDSIPKSPSGKILRRQLRALLA SI
Sbjct: 541 INRVFFVDSIPKSPSGKILRRQLRALLAASI 550

BLAST of Lsi06G011330 vs. NCBI nr
Match: KAA0058290.1 (4-coumarate--CoA ligase 1-like [Cucumis melo var. makuwa])

HSP 1 Score: 926.8 bits (2394), Expect = 9.7e-266
Identity = 483/573 (84.29%), Postives = 506/573 (88.31%), Query Frame = 0

Query: 40  MAAADDPSLEFIFRSKLPQIPIPTHLPLHTYCFEKISEFKHRPCLINAATGESYTYGEVQ 99
           MAAAD  S EF+FRSKLP+IPI THLPLHTYCFE ISEFK RPCLINAATG +YTYGEVQ
Sbjct: 1   MAAADGSSPEFLFRSKLPEIPISTHLPLHTYCFENISEFKQRPCLINAATGHTYTYGEVQ 60

Query: 100 ETSRRVAAGLHKLGIGKGDVIMLLLQNTPQFVLAFLGTSYLGAAATTVNPFFMPAEIAKQ 159
             SRRVAAGLHKLGIGKGDVIMLLLQNTP+FVLAFL  SYLGAAAT  NPFFMPAEIAKQ
Sbjct: 61  AISRRVAAGLHKLGIGKGDVIMLLLQNTPEFVLAFLAASYLGAAATMANPFFMPAEIAKQ 120

Query: 160 AASSGAKLIITQAAFAEKVKKLSQENKATIKVIFI-DDDPPLEGGYHFSSLIDDVAK--- 219
           A SSGAK+IITQ+AFAEKVKKLSQ ++  IKVIFI DDDPP +GGY FSSLIDDVAK   
Sbjct: 121 AVSSGAKVIITQSAFAEKVKKLSQGSEMIIKVIFIDDDDPPTKGGYKFSSLIDDVAKEEE 180

Query: 220 VEMEDVKISPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPHLYIRSDDVVL 279
           +EM DVKISPDDVVALPYSSGTTGLPKGVMLTHKG VTSVAQQVDGENPHLYIRSDDVVL
Sbjct: 181 LEMGDVKISPDDVVALPYSSGTTGLPKGVMLTHKGSVTSVAQQVDGENPHLYIRSDDVVL 240

Query: 280 CVLPLFHIYSLNTIMMCSLRVGAAILIVRKFDVNSLVELIPKYRVTFAPFVPPIVLAIVK 339
           C+LPLFHIYSLN+IMMCSLRVGAAILIV+KFD+NSL+EL+PKY+VTFAP VPPIVLAI K
Sbjct: 241 CLLPLFHIYSLNSIMMCSLRVGAAILIVQKFDINSLLELVPKYKVTFAPVVPPIVLAIAK 300

Query: 340 SPAVDHSDMSSLRMALSGAAPLGKNLEDAFRTKFSHVILGQGYGMTELGSVMTMSLAFAK 399
           SPAVDH DMSSLRM LSGAAPLGKNLEDAFR K  HVILGQGYGMTE GSVMTMSLAFAK
Sbjct: 301 SPAVDHFDMSSLRMVLSGAAPLGKNLEDAFRAKLPHVILGQGYGMTEAGSVMTMSLAFAK 360

Query: 400 ERFEMKSGGCGTIMRNTEMKIINPQTGASLPRNQAGEICIRSPQMMKGYLNDEEATKGII 459
           E F +KSGGCGTIMRN+EMKI+ PQTG SLPRN+ GEICIRS QMMKGYLNDEEATK II
Sbjct: 361 EGFGIKSGGCGTIMRNSEMKIVCPQTGVSLPRNRTGEICIRSFQMMKGYLNDEEATKAII 420

Query: 460 DKDGWLHTGDIGFVDDDDEVFIVDRLKELIKYKGFQVAPAELEALLISHGHIADAAVIPM 519
           DKDGWLHTGDIG+VDDDDE+FIVDRLKELIKYKGFQVAPAELEALLISHGHIADAAVIPM
Sbjct: 421 DKDGWLHTGDIGYVDDDDELFIVDRLKELIKYKGFQVAPAELEALLISHGHIADAAVIPM 480

Query: 520 KDEVAGEVPVAFIVLSDGSNITEDEIKQYISKQFSGLISHYLLPIDGDFCIDFTVVFYKR 579
            DEVAGEVPV FIV  DGSNITEDEIKQYISKQ                     VVFYKR
Sbjct: 481 NDEVAGEVPVTFIVRFDGSNITEDEIKQYISKQ---------------------VVFYKR 540

Query: 580 INRVFFVDSIPKSPSGKILRRQLRALLATSIPN 609
           INRVFFVDSIPKSPSGKILRRQLRALLA SI N
Sbjct: 541 INRVFFVDSIPKSPSGKILRRQLRALLAASILN 552

BLAST of Lsi06G011330 vs. NCBI nr
Match: XP_004146311.1 (4-coumarate--CoA ligase 1 [Cucumis sativus] >KGN65444.1 hypothetical protein Csa_019996 [Cucumis sativus])

HSP 1 Score: 909.4 bits (2349), Expect = 1.6e-260
Identity = 478/571 (83.71%), Postives = 502/571 (87.92%), Query Frame = 0

Query: 40  MAAADDPSLEFIFRSKLPQIPIPTHLPLHTYCFEKISEFKHRPCLINAATGESYTYGEVQ 99
           MAAAD  S EFIFRSKLP+IPI THLPLHTYCFE ISEFKHRPCLINAATG  YTYGEVQ
Sbjct: 1   MAAADASSPEFIFRSKLPEIPISTHLPLHTYCFENISEFKHRPCLINAATGHIYTYGEVQ 60

Query: 100 ETSRRVAAGLHKLGIGKGDVIMLLLQNTPQFVLAFLGTSYLGAAATTVNPFFMPAEIAKQ 159
            TSRRVAAGLHKLGIGKGDVIMLLLQNTP+FVLAFLG SYLGAAAT  NPFFM +EIAKQ
Sbjct: 61  ATSRRVAAGLHKLGIGKGDVIMLLLQNTPEFVLAFLGASYLGAAATMANPFFMQSEIAKQ 120

Query: 160 AASSGAKLIITQAAFAEKVKKLSQENKATIKVIFIDD-DPPLEGGYHFSSLIDDVAK--- 219
           A SSGAK+IITQAAFAEKVK LSQEN+  IKVIFIDD DPP      FSSLI+DVAK   
Sbjct: 121 AVSSGAKVIITQAAFAEKVKNLSQENEMIIKVIFIDDNDPP-----QFSSLIEDVAKEEE 180

Query: 220 VEMEDVKISPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPHLYIRSDDVVL 279
           +EM DVKISP+DVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPHL+IRSDDVVL
Sbjct: 181 LEMGDVKISPEDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPHLHIRSDDVVL 240

Query: 280 CVLPLFHIYSLNTIMMCSLRVGAAILIVRKFDVNSLVELIPKYRVTFAPFVPPIVLAIVK 339
           C+LPLFHIYSLN+IMMC+LRVGAAIL+V+KFDVNSLVEL+ KY+VTFAPFVPPIVLAI K
Sbjct: 241 CLLPLFHIYSLNSIMMCALRVGAAILLVQKFDVNSLVELVSKYKVTFAPFVPPIVLAIAK 300

Query: 340 SPAVDHSDMSSLRMALSGAAPLGKNLEDAFRTKFSHVILGQGYGMTELGSVMTMSLAFAK 399
           SPAVDH DMSSLR+ LSGAAPLGKNLEDAFR K  HVILGQGYGMTE GSVMTMSLAF K
Sbjct: 301 SPAVDHFDMSSLRIVLSGAAPLGKNLEDAFRAKLPHVILGQGYGMTEAGSVMTMSLAFVK 360

Query: 400 ERFEMKSGGCGTIMRNTEMKIINPQTGASLPRNQAGEICIRSPQMMKGYLNDEEATKGII 459
           E F +KSGGCGTIMRN+EMKI+N QTG SLPRNQ GEICIRS QMMKGYLNDE+ATK II
Sbjct: 361 EGFGIKSGGCGTIMRNSEMKILNLQTGESLPRNQTGEICIRSSQMMKGYLNDEDATKAII 420

Query: 460 DKDGWLHTGDIGFVDDDDEVFIVDRLKELIKYKGFQVAPAELEALLISHGHIADAAVIPM 519
           D+DGWLHTGDIGFVDDDDE+FIVDRLKELIKYKGFQVAPAELEALLISH HIADAAVIPM
Sbjct: 421 DEDGWLHTGDIGFVDDDDELFIVDRLKELIKYKGFQVAPAELEALLISHAHIADAAVIPM 480

Query: 520 KDEVAGEVPVAFIVLSDGSNITEDEIKQYISKQFSGLISHYLLPIDGDFCIDFTVVFYKR 579
            DEVAGEVPVAFIV  DGSNITEDEIKQ+ISKQ                     VVFYKR
Sbjct: 481 NDEVAGEVPVAFIVRFDGSNITEDEIKQFISKQ---------------------VVFYKR 540

Query: 580 INRVFFVDSIPKSPSGKILRRQLRALLATSI 607
           INRVFFVDSIPKSPSGKILRRQLRALLA SI
Sbjct: 541 INRVFFVDSIPKSPSGKILRRQLRALLAASI 545

BLAST of Lsi06G011330 vs. NCBI nr
Match: XP_023515445.1 (4-coumarate--CoA ligase 1-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 874.8 bits (2259), Expect = 4.4e-250
Identity = 452/567 (79.72%), Postives = 482/567 (85.01%), Query Frame = 0

Query: 42  AADDPSLEFIFRSKLPQIPIPTHLPLHTYCFEKISEFKHRPCLINAATGESYTYGEVQET 101
           AA DPS EFIFRSKLP + IP HLPLHTYCFE+ISEFKH PCLINAATG+ YTYGEV  T
Sbjct: 2   AAADPSPEFIFRSKLPDLTIPNHLPLHTYCFERISEFKHNPCLINAATGDIYTYGEVHVT 61

Query: 102 SRRVAAGLHKLGIGKGDVIMLLLQNTPQFVLAFLGTSYLGAAATTVNPFFMPAEIAKQAA 161
           +RRVAAGLHKLGIGKGDVIMLLLQNTPQFV AFLG S+  A AT  NPFF PAEI KQAA
Sbjct: 62  ARRVAAGLHKLGIGKGDVIMLLLQNTPQFVFAFLGASFACAIATMANPFFTPAEIGKQAA 121

Query: 162 SSGAKLIITQAAFAEKVKKLSQENKATIKVIFIDDDPPLEGGYHFSSLIDDVAKVEMEDV 221
           SSGAKLIITQAAFAEKVK L QEN+  +KV+FID  P  EGG HFSSL D+  + EM DV
Sbjct: 122 SSGAKLIITQAAFAEKVKMLPQENQGILKVVFIDSPP--EGGCHFSSLTDEAKEEEMADV 181

Query: 222 KISPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPHLYIRSDDVVLCVLPLF 281
           K SPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPH +IRS+DVVLCVLPLF
Sbjct: 182 KFSPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPHFHIRSNDVVLCVLPLF 241

Query: 282 HIYSLNTIMMCSLRVGAAILIVRKFDVNSLVELIPKYRVTFAPFVPPIVLAIVKSPAVDH 341
           HIYSL++I+M SLRVGAAILIV+KFD+NSLV L+PKYRVT A  VPPIVLA+VKSP VDH
Sbjct: 242 HIYSLSSIVMTSLRVGAAILIVQKFDINSLVALVPKYRVTIATVVPPIVLAMVKSPVVDH 301

Query: 342 SDMSSLRMALSGAAPLGKNLEDAFRTKFSHVILGQGYGMTELGSVMTMSLAFAKERFEMK 401
           SDMSS+R+ LSGAAPLGKNLEDAFR K  H ILGQGYGMTE GSV+TMSLA AKERF +K
Sbjct: 302 SDMSSIRIVLSGAAPLGKNLEDAFRAKLPHAILGQGYGMTESGSVLTMSLALAKERFAIK 361

Query: 402 SGGCGTIMRNTEMKIINPQTGASLPRNQAGEICIRSPQMMKGYLNDEEATKGIIDKDGWL 461
           SG CGT+MRN EMKI+NP TG SL +NQ GEI ++SPQMMKGYLNDEEATKGIID+ GWL
Sbjct: 362 SGACGTLMRNAEMKIVNPDTGVSLSKNQPGEIYVKSPQMMKGYLNDEEATKGIIDEHGWL 421

Query: 462 HTGDIGFVDDDDEVFIVDRLKELIKYKGFQVAPAELEALLISHGHIADAAVIPMKDEVAG 521
           HTGDIG VDDDDEVFIVDRLKELIKYKGFQVAPAELE LLIS+ HI D AVIPMKDEVAG
Sbjct: 422 HTGDIGLVDDDDEVFIVDRLKELIKYKGFQVAPAELEGLLISNVHIVDVAVIPMKDEVAG 481

Query: 522 EVPVAFIVLSDGSNITEDEIKQYISKQFSGLISHYLLPIDGDFCIDFTVVFYKRINRVFF 581
           EVPVAFIV SDGSNITEDEIKQYISKQ                     VVFYKRINRVFF
Sbjct: 482 EVPVAFIVRSDGSNITEDEIKQYISKQ---------------------VVFYKRINRVFF 541

Query: 582 VDSIPKSPSGKILRRQLRALLATSIPN 609
           VDSIPKSPSGKILRRQLRALL  SIPN
Sbjct: 542 VDSIPKSPSGKILRRQLRALLPASIPN 545

BLAST of Lsi06G011330 vs. TAIR 10
Match: AT3G21240.1 (4-coumarate:CoA ligase 2 )

HSP 1 Score: 684.1 bits (1764), Expect = 1.0e-196
Identity = 352/563 (62.52%), Postives = 421/563 (74.78%), Query Frame = 0

Query: 47  SLEFIFRSKLPQIPIPTHLPLHTYCFEKISEFKHRPCLINAATGESYTYGEVQETSRRVA 106
           S + IFRS+LP I IP HLPLH Y FE ISEF  +PCLIN  TGE YTY +V  TSR++A
Sbjct: 18  SNDVIFRSRLPDIYIPNHLPLHDYIFENISEFAAKPCLINGPTGEVYTYADVHVTSRKLA 77

Query: 107 AGLHKLGIGKGDVIMLLLQNTPQFVLAFLGTSYLGAAATTVNPFFMPAEIAKQAASSGAK 166
           AGLH LG+ + DV+M+LL N+P+ VL FL  S++GA  T+ NPFF PAEI+KQA +S AK
Sbjct: 78  AGLHNLGVKQHDVVMILLPNSPEVVLTFLAASFIGAITTSANPFFTPAEISKQAKASAAK 137

Query: 167 LIITQAAFAEKVKKLSQENKATIKVIFIDDDPPLEGGYHFSSLI-DDVAKVEMEDVKISP 226
           LI+TQ+ + +K+K L  +    + ++  D D   E    FS L   +  +V+    KISP
Sbjct: 138 LIVTQSRYVDKIKNLQNDG---VLIVTTDSDAIPENCLRFSELTQSEEPRVDSIPEKISP 197

Query: 227 DDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPHLYIRSDDVVLCVLPLFHIYS 286
           +DVVALP+SSGTTGLPKGVMLTHKGLVTSVAQQVDGENP+LY   DDV+LCVLP+FHIY+
Sbjct: 198 EDVVALPFSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFNRDDVILCVLPMFHIYA 257

Query: 287 LNTIMMCSLRVGAAILIVRKFDVNSLVELIPKYRVTFAPFVPPIVLAIVKSPAVDHSDMS 346
           LN+IM+CSLRVGA ILI+ KF++  L+E I + +VT A  VPPIVLAI KSP  +  D+S
Sbjct: 258 LNSIMLCSLRVGATILIMPKFEITLLLEQIQRCKVTVAMVVPPIVLAIAKSPETEKYDLS 317

Query: 347 SLRMALSGAAPLGKNLEDAFRTKFSHVILGQGYGMTELGSVMTMSLAFAKERFEMKSGGC 406
           S+RM  SGAAPLGK LEDA   KF +  LGQGYGMTE G V+ MSL FAKE F +KSG C
Sbjct: 318 SVRMVKSGAAPLGKELEDAISAKFPNAKLGQGYGMTEAGPVLAMSLGFAKEPFPVKSGAC 377

Query: 407 GTIMRNTEMKIINPQTGASLPRNQAGEICIRSPQMMKGYLNDEEATKGIIDKDGWLHTGD 466
           GT++RN EMKI++P TG SLPRN+ GEICIR  Q+MKGYLND  AT   IDKDGWLHTGD
Sbjct: 378 GTVVRNAEMKILDPDTGDSLPRNKPGEICIRGNQIMKGYLNDPLATASTIDKDGWLHTGD 437

Query: 467 IGFVDDDDEVFIVDRLKELIKYKGFQVAPAELEALLISHGHIADAAVIPMKDEVAGEVPV 526
           +GF+DDDDE+FIVDRLKELIKYKGFQVAPAELE+LLI H  I D AV+ MK+E AGEVPV
Sbjct: 438 VGFIDDDDELFIVDRLKELIKYKGFQVAPAELESLLIGHPEINDVAVVAMKEEDAGEVPV 497

Query: 527 AFIVLSDGSNITEDEIKQYISKQFSGLISHYLLPIDGDFCIDFTVVFYKRINRVFFVDSI 586
           AF+V S  SNI+EDEIKQ++SKQ                     VVFYKRIN+VFF DSI
Sbjct: 498 AFVVRSKDSNISEDEIKQFVSKQ---------------------VVFYKRINKVFFTDSI 556

Query: 587 PKSPSGKILRRQLRALLATSIPN 609
           PK+PSGKILR+ LRA LA  + N
Sbjct: 558 PKAPSGKILRKDLRARLANGLMN 556

BLAST of Lsi06G011330 vs. TAIR 10
Match: AT1G51680.1 (4-coumarate:CoA ligase 1 )

HSP 1 Score: 681.4 bits (1757), Expect = 6.6e-196
Identity = 351/563 (62.34%), Postives = 419/563 (74.42%), Query Frame = 0

Query: 49  EFIFRSKLPQIPIPTHLPLHTYCFEKISEFKHRPCLINAATGESYTYGEVQETSRRVAAG 108
           + IFRSKLP I IP HL LH Y F+ ISEF  +PCLIN  TG  YTY +V   SR++AA 
Sbjct: 22  DVIFRSKLPDIYIPNHLSLHDYIFQNISEFATKPCLINGPTGHVYTYSDVHVISRQIAAN 81

Query: 109 LHKLGIGKGDVIMLLLQNTPQFVLAFLGTSYLGAAATTVNPFFMPAEIAKQAASSGAKLI 168
            HKLG+ + DV+MLLL N P+FVL+FL  S+ GA AT  NPFF PAEIAKQA +S  KLI
Sbjct: 82  FHKLGVNQNDVVMLLLPNCPEFVLSFLAASFRGATATAANPFFTPAEIAKQAKASNTKLI 141

Query: 169 ITQAAFAEKVKKLSQENKATIKVIFIDDD---PPLEGGYHFSSLIDDVAKVE--MEDVKI 228
           IT+A + +K+K L  +N   + ++ IDD+   P  EG   F+ L     +    ++ V+I
Sbjct: 142 ITEARYVDKIKPL--QNDDGVVIVCIDDNESVPIPEGCLRFTELTQSTTEASEVIDSVEI 201

Query: 229 SPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPHLYIRSDDVVLCVLPLFHI 288
           SPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENP+LY  SDDV+LCVLP+FHI
Sbjct: 202 SPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFHSDDVILCVLPMFHI 261

Query: 289 YSLNTIMMCSLRVGAAILIVRKFDVNSLVELIPKYRVTFAPFVPPIVLAIVKSPAVDHSD 348
           Y+LN+IM+C LRVGAAILI+ KF++N L+ELI + +VT AP VPPIVLAI KS   +  D
Sbjct: 262 YALNSIMLCGLRVGAAILIMPKFEINLLLELIQRCKVTVAPMVPPIVLAIAKSSETEKYD 321

Query: 349 MSSLRMALSGAAPLGKNLEDAFRTKFSHVILGQGYGMTELGSVMTMSLAFAKERFEMKSG 408
           +SS+R+  SGAAPLGK LEDA   KF +  LGQGYGMTE G V+ MSL FAKE F +KSG
Sbjct: 322 LSSIRVVKSGAAPLGKELEDAVNAKFPNAKLGQGYGMTEAGPVLAMSLGFAKEPFPVKSG 381

Query: 409 GCGTIMRNTEMKIINPQTGASLPRNQAGEICIRSPQMMKGYLNDEEATKGIIDKDGWLHT 468
            CGT++RN EMKI++P TG SL RNQ GEICIR  Q+MKGYLN+  AT   IDKDGWLHT
Sbjct: 382 ACGTVVRNAEMKIVDPDTGDSLSRNQPGEICIRGHQIMKGYLNNPAATAETIDKDGWLHT 441

Query: 469 GDIGFVDDDDEVFIVDRLKELIKYKGFQVAPAELEALLISHGHIADAAVIPMKDEVAGEV 528
           GDIG +DDDDE+FIVDRLKELIKYKGFQVAPAELEALLI H  I D AV+ MK+E AGEV
Sbjct: 442 GDIGLIDDDDELFIVDRLKELIKYKGFQVAPAELEALLIGHPDITDVAVVAMKEEAAGEV 501

Query: 529 PVAFIVLSDGSNITEDEIKQYISKQFSGLISHYLLPIDGDFCIDFTVVFYKRINRVFFVD 588
           PVAF+V S  S ++ED++KQ++SKQ                     VVFYKRIN+VFF +
Sbjct: 502 PVAFVVKSKDSELSEDDVKQFVSKQ---------------------VVFYKRINKVFFTE 561

Query: 589 SIPKSPSGKILRRQLRALLATSI 607
           SIPK+PSGKILR+ LRA LA  +
Sbjct: 562 SIPKAPSGKILRKDLRAKLANGL 561

BLAST of Lsi06G011330 vs. TAIR 10
Match: AT1G65060.1 (4-coumarate:CoA ligase 3 )

HSP 1 Score: 649.4 bits (1674), Expect = 2.8e-186
Identity = 329/572 (57.52%), Postives = 420/572 (73.43%), Query Frame = 0

Query: 32  TSTSATPPMAAADDPSLEFIFRSKLPQIPIPTHLPLHTYCFEKISEFKHRPCLINAATGE 91
           T TS          P    IFRSKLP I IP HLPLHTYCFEK+S    +PCLI  +TG+
Sbjct: 15  TDTSVVSDDVLPHSPPTPRIFRSKLPDIDIPNHLPLHTYCFEKLSSVSDKPCLIVGSTGK 74

Query: 92  SYTYGEVQETSRRVAAGLHKLGIGKGDVIMLLLQNTPQFVLAFLGTSYLGAAATTVNPFF 151
           SYTYGE     RRVA+GL+KLGI KGDVIM+LLQN+ +FV +F+G S +GA +TT NPF+
Sbjct: 75  SYTYGETHLICRRVASGLYKLGIRKGDVIMILLQNSAEFVFSFMGASMIGAVSTTANPFY 134

Query: 152 MPAEIAKQAASSGAKLIITQAAFAEKVKKLSQENKATIKVIFIDDDPPLEGGYHFSSLI- 211
              E+ KQ  SSGAKLIIT + + +K+K L +        +   D+P  E    FS+LI 
Sbjct: 135 TSQELYKQLKSSGAKLIITHSQYVDKLKNLGEN-----LTLITTDEPTPENCLPFSTLIT 194

Query: 212 DDVAKVEMEDVKISPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPHLYIRS 271
           DD      E V I  DD  ALP+SSGTTGLPKGV+LTHK L+TSVAQQVDG+NP+LY++S
Sbjct: 195 DDETNPFQETVDIGGDDAAALPFSSGTTGLPKGVVLTHKSLITSVAQQVDGDNPNLYLKS 254

Query: 272 DDVVLCVLPLFHIYSLNTIMMCSLRVGAAILIVRKFDVNSLVELIPKYRVTFAPFVPPIV 331
           +DV+LCVLPLFHIYSLN++++ SLR GA +L++ KF++ +L++LI ++RVT A  VPP+V
Sbjct: 255 NDVILCVLPLFHIYSLNSVLLNSLRSGATVLLMHKFEIGALLDLIQRHRVTIAALVPPLV 314

Query: 332 LAIVKSPAVDHSDMSSLRMALSGAAPLGKNLEDAFRTKFSHVILGQGYGMTELGSVMTMS 391
           +A+ K+P V+  D+SS+R  LSGAAPLGK L+D+ R +    ILGQGYGMTE G V++MS
Sbjct: 315 IALAKNPTVNSYDLSSVRFVLSGAAPLGKELQDSLRRRLPQAILGQGYGMTEAGPVLSMS 374

Query: 392 LAFAKERFEMKSGGCGTIMRNTEMKIINPQTGASLPRNQAGEICIRSPQMMKGYLNDEEA 451
           L FAKE    KSG CGT++RN E+K+++ +T  SL  NQ GEICIR  Q+MK YLND EA
Sbjct: 375 LGFAKEPIPTKSGSCGTVVRNAELKVVHLETRLSLGYNQPGEICIRGQQIMKEYLNDPEA 434

Query: 452 TKGIIDKDGWLHTGDIGFVDDDDEVFIVDRLKELIKYKGFQVAPAELEALLISHGHIADA 511
           T   ID++GWLHTGDIG+VD+DDE+FIVDRLKE+IK+KGFQV PAELE+LLI+H  IADA
Sbjct: 435 TSATIDEEGWLHTGDIGYVDEDDEIFIVDRLKEVIKFKGFQVPPAELESLLINHHSIADA 494

Query: 512 AVIPMKDEVAGEVPVAFIVLSDGSNITEDEIKQYISKQFSGLISHYLLPIDGDFCIDFTV 571
           AV+P  DEVAGEVPVAF+V S+G++ITE+++K+Y++KQ                     V
Sbjct: 495 AVVPQNDEVAGEVPVAFVVRSNGNDITEEDVKEYVAKQ---------------------V 554

Query: 572 VFYKRINRVFFVDSIPKSPSGKILRRQLRALL 603
           VFYKR+++VFFV SIPKSPSGKILR+ L+A L
Sbjct: 555 VFYKRLHKVFFVASIPKSPSGKILRKDLKAKL 560

BLAST of Lsi06G011330 vs. TAIR 10
Match: AT1G51680.3 (4-coumarate:CoA ligase 1 )

HSP 1 Score: 639.4 bits (1648), Expect = 2.9e-183
Identity = 324/510 (63.53%), Postives = 388/510 (76.08%), Query Frame = 0

Query: 49  EFIFRSKLPQIPIPTHLPLHTYCFEKISEFKHRPCLINAATGESYTYGEVQETSRRVAAG 108
           + IFRSKLP I IP HL LH Y F+ ISEF  +PCLIN  TG  YTY +V   SR++AA 
Sbjct: 22  DVIFRSKLPDIYIPNHLSLHDYIFQNISEFATKPCLINGPTGHVYTYSDVHVISRQIAAN 81

Query: 109 LHKLGIGKGDVIMLLLQNTPQFVLAFLGTSYLGAAATTVNPFFMPAEIAKQAASSGAKLI 168
            HKLG+ + DV+MLLL N P+FVL+FL  S+ GA AT  NPFF PAEIAKQA +S  KLI
Sbjct: 82  FHKLGVNQNDVVMLLLPNCPEFVLSFLAASFRGATATAANPFFTPAEIAKQAKASNTKLI 141

Query: 169 ITQAAFAEKVKKLSQENKATIKVIFIDDD---PPLEGGYHFSSLIDDVAKVE--MEDVKI 228
           IT+A + +K+K L  +N   + ++ IDD+   P  EG   F+ L     +    ++ V+I
Sbjct: 142 ITEARYVDKIKPL--QNDDGVVIVCIDDNESVPIPEGCLRFTELTQSTTEASEVIDSVEI 201

Query: 229 SPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPHLYIRSDDVVLCVLPLFHI 288
           SPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENP+LY  SDDV+LCVLP+FHI
Sbjct: 202 SPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFHSDDVILCVLPMFHI 261

Query: 289 YSLNTIMMCSLRVGAAILIVRKFDVNSLVELIPKYRVTFAPFVPPIVLAIVKSPAVDHSD 348
           Y+LN+IM+C LRVGAAILI+ KF++N L+ELI + +VT AP VPPIVLAI KS   +  D
Sbjct: 262 YALNSIMLCGLRVGAAILIMPKFEINLLLELIQRCKVTVAPMVPPIVLAIAKSSETEKYD 321

Query: 349 MSSLRMALSGAAPLGKNLEDAFRTKFSHVILGQGYGMTELGSVMTMSLAFAKERFEMKSG 408
           +SS+R+  SGAAPLGK LEDA   KF +  LGQGYGMTE G V+ MSL FAKE F +KSG
Sbjct: 322 LSSIRVVKSGAAPLGKELEDAVNAKFPNAKLGQGYGMTEAGPVLAMSLGFAKEPFPVKSG 381

Query: 409 GCGTIMRNTEMKIINPQTGASLPRNQAGEICIRSPQMMKGYLNDEEATKGIIDKDGWLHT 468
            CGT++RN EMKI++P TG SL RNQ GEICIR  Q+MKGYLN+  AT   IDKDGWLHT
Sbjct: 382 ACGTVVRNAEMKIVDPDTGDSLSRNQPGEICIRGHQIMKGYLNNPAATAETIDKDGWLHT 441

Query: 469 GDIGFVDDDDEVFIVDRLKELIKYKGFQVAPAELEALLISHGHIADAAVIPMKDEVAGEV 528
           GDIG +DDDDE+FIVDRLKELIKYKGFQVAPAELEALLI H  I D AV+ MK+E AGEV
Sbjct: 442 GDIGLIDDDDELFIVDRLKELIKYKGFQVAPAELEALLIGHPDITDVAVVAMKEEAAGEV 501

Query: 529 PVAFIVLSDGSNITEDEIKQYISKQFSGLI 554
           PVAF+V S  S ++ED++KQ++SKQ    +
Sbjct: 502 PVAFVVKSKDSELSEDDVKQFVSKQVKSCV 529

BLAST of Lsi06G011330 vs. TAIR 10
Match: AT3G21230.1 (4-coumarate:CoA ligase 5 )

HSP 1 Score: 621.3 bits (1601), Expect = 8.1e-178
Identity = 322/572 (56.29%), Postives = 411/572 (71.85%), Query Frame = 0

Query: 44  DDPSLEFIFRSKLPQIPIPTHLPLHTYCFEKIS----EFKHRPCLINAATGESYTYGEVQ 103
           ++PS +FIFRSKLP I IP HLPL  Y F++ S          C+I+ ATG   TY +VQ
Sbjct: 21  EEPSHDFIFRSKLPDIFIPNHLPLTDYVFQRFSGDGDGDSSTTCIIDGATGRILTYADVQ 80

Query: 104 ETSRRVAAGLHKLGIGKGDVIMLLLQNTPQFVLAFLGTSYLGAAATTVNPFFMPAEIAKQ 163
              RR+AAG+H+LGI  GDV+MLLL N+P+F L+FL  +YLGA +TT NPF+   EIAKQ
Sbjct: 81  TNMRRIAAGIHRLGIRHGDVVMLLLPNSPEFALSFLAVAYLGAVSTTANPFYTQPEIAKQ 140

Query: 164 AASSGAKLIITQAAFAEKVKKLSQENKATIKVIFIDDD-------PPLEGGYHFSSLIDD 223
           A +S AK+IIT+    +K+  L  +    + ++ +DDD          +G   F+ L   
Sbjct: 141 AKASAAKMIITKKCLVDKLTNLKNDG---VLIVCLDDDGDNGVVSSSDDGCVSFTEL-TQ 200

Query: 224 VAKVEMEDVKISPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPHLYIRSDD 283
             + E+   KISP+D VA+PYSSGTTGLPKGVM+THKGLVTS+AQ+VDGENP+L   ++D
Sbjct: 201 ADETELLKPKISPEDTVAMPYSSGTTGLPKGVMITHKGLVTSIAQKVDGENPNLNFTAND 260

Query: 284 VVLCVLPLFHIYSLNTIMMCSLRVGAAILIVRKFDVNSLVELIPKYRVTFAPFVPPIVLA 343
           V+LC LP+FHIY+L+ +M+ ++R GAA+LIV +F++N ++ELI +Y+VT  P  PP+VLA
Sbjct: 261 VILCFLPMFHIYALDALMLSAMRTGAALLIVPRFELNLVMELIQRYKVTVVPVAPPVVLA 320

Query: 344 IVKSPAVDHSDMSSLRMALSGAAPLGKNLEDAFRTKFSHVILGQGYGMTELGSVMTMSLA 403
            +KSP  +  D+SS+R+ LSGAA L K LEDA R KF + I GQGYGMTE G+V   SLA
Sbjct: 321 FIKSPETERYDLSSVRIMLSGAATLKKELEDAVRLKFPNAIFGQGYGMTESGTV-AKSLA 380

Query: 404 FAKERFEMKSGGCGTIMRNTEMKIINPQTGASLPRNQAGEICIRSPQMMKGYLNDEEATK 463
           FAK  F+ KSG CGT++RN EMK+++ +TG SLPRN++GEIC+R  Q+MKGYLND EAT 
Sbjct: 381 FAKNPFKTKSGACGTVIRNAEMKVVDTETGISLPRNKSGEICVRGHQLMKGYLNDPEATA 440

Query: 464 GIIDKDGWLHTGDIGFVDDDDEVFIVDRLKELIKYKGFQVAPAELEALLISHGHIADAAV 523
             IDKDGWLHTGDIGFVDDDDE+FIVDRLKELIK+KG+QVAPAELEALLISH  I DAAV
Sbjct: 441 RTIDKDGWLHTGDIGFVDDDDEIFIVDRLKELIKFKGYQVAPAELEALLISHPSIDDAAV 500

Query: 524 IPMKDEVAGEVPVAFIVLSDGSNITEDEIKQYISKQFSGLISHYLLPIDGDFCIDFTVVF 583
           + MKDEVA EVPVAF+  S GS +TED++K Y++KQ                     VV 
Sbjct: 501 VAMKDEVADEVPVAFVARSQGSQLTEDDVKSYVNKQ---------------------VVH 560

Query: 584 YKRINRVFFVDSIPKSPSGKILRRQLRALLAT 605
           YKRI  VFF++ IPK+ SGKILR+ LRA L T
Sbjct: 561 YKRIKMVFFIEVIPKAVSGKILRKDLRAKLET 566