Lsi06G009200 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi06G009200
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	vacuolar protein sorting-associated protein 25
Location	chr06: 18962728 .. 18966651 (-)
RNA-Seq Expression	Lsi06G009200
Synteny	Lsi06G009200

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCAGAAATTGGGAGATTTCAAGCTACCACATTTCTTCAATTATCCTCCATATTTCACGTAATTTTCATCATCTGTTTCAGTTATCATGTTTGTTACATCTTTATTAGCTACACAAGTAACTTGGAAGCTGTGTGAGTCAATTTCTGATGATGTTGATATCTTGGTTTTGACGACAATTTCCTGAGCGAAGAAAACTGACACTTCACTGCTCAACTAGTAGTTACACAACTGTAAACGGAAACTGCATATACTGGATCCCTTCTGCTTTCCCTACCAGCCTCATATATACGAATGTGATAGTTGTAAAGTTGTACCTTACGGCAATCCTGCAATTCTTATTGGATAACGTTGATTGTTTCACTTTTCTCAAGCTTGTCTTCTGTCTTGGGTTGCATATATAACCTTTTCATTGCATGTCGCCTCAGTTTGCAACCAGTGCGGGACACCCGGGAGAAGCAAATTCAACTTTGGAAGGAACTTATCCTCGATTACTGCCGAACGCAAAAGATATTTGTCATTGGACTCGAAGAGGACTTTCCTCTATTTTCAAATTCTGAGATTGAAAGTAAATTTCATAACCTTGTTTGGTCCTAATTGGCTGTTAAAACTTCTTGGTGTTTTAGACTCAGTCACGAAGCTGTGTATTACTGTTGCAGTTGTTTGATGTAATTTACAGTGTGGTTGTGATGCTACAGGATCTCTCAGTCATGAAGCTAGAGAAGCTTTTCTCTCAGCTTTAGTTTCAGATGGTTAGGGCCTGCTAGTTTAGTTCTATAATTCTATCTGTTTACAGTTTTCATTCTTTTTCATAGGGAAAAAACTGATACTTGTTCATTTTAATTGTCCTATAGGTCGTGCAGAATGGCTGGATAAAGGACACAGGAAATGCCTTATTTTATGGCACCGGATTCAAGATTGGGCTGACATCATTTTGCACTTTGTAAGAACTAAATACCTTGGTTAAATTATAAGTTTAGTCTCAAAATTTTGATATGTCTATTTGCTCCATGAATTTTCAAAAGTGGCCAATAGATCAAACCTTTGACAAATTTGAAAATTTTCAAAATTACTTAATCTATTAGATGTGATTTTATGCTAATGGAATTCTAAACTTTCACTTTTGTGTCTAGTAAATTTGCGAAAATTCAAAAGTTTAGGGACTAAATTTATAATTTTGAAAGTTCAAGGACTAAATAGACACAAATTTCAAAATTAAGGAACTGAATTTGTAATTTAACCAAATATTTTATTAACTATCATAGTTATGAGTGAGTTTGGAATGATTTTGAAAGGTAAAAAATTCCACAAGTATTTTTATAGAAAATCAGAAGTGCTTTGAAAAATTCTTATTCTTAAATAATCTCCTATAAATAGGTGAAAAATAATGGACTTGAAGATAGTGTTATGACCATCGAGGAGATACGTTCAGGAGTTGAGTCTAGAGGAACAGGTACGTCCCCTTCTTATACTCAAGGTCTTCAAGAACGAGATATTTGTTTCTTTAAACAAATAAAAATATAAAAACAAAAAATTCAAAAATGAAATATTTAGGTTAATAACTAATCATTTTAAACTTTTTAAACAAGTTAAAATGTTGGGCAAAATGCATTTTTAGTCCCCGAGGTTTGAGCTTGATGTCTATTTGGTCCTTGAAGTTTCAAAATGAACACTTTAGTCCTCGAGGTTTGGAAAATATTTCTAAATGGTCCCTGAGTTAATTTGACCGTTAGTTAACGGAAAAGTGGTAAATTTTAAATGAGGTGACATTTGTACTATTTTTTTTTTTTAATTTTCTATATTATGTGGATTTTTTTTCTCTTTCCTTTGTCTCTCTCCTCCCTTCCCTTTCCCCTCTCCCATCCATGGAATCCCCTTAGTTTCTCTACTCCCTTCTTCTTTCTCTCTCTCCTCCTAGTATGGATTAAAAAAATAGCATATCTGTTTTCACTCCCCTCCATGTCTGCATAAAAAAAAAAATCACATATCTGTTTTCACTCATCCCTAAAATAGCAAGCACAAATCCAACTAAATCTCCAAATATATCTCCAAAAATACTAAAAAAAAACTTTAAAAATCGGAGTGATAATTGATAGGAGCATCTCCATTTTAAGCTTTAGGTCAAAGTTTCTAATTGTCTAAAATTATAATTTAACCACATAATTATATCACATCACCAAGAGTTTAGGCAAAATGATAGTGCTTAAATTAGATTCGACATTTATATATACATTTAGTAGTGTTTTTTAGATTTGGTTTTTTTTTTTTTTGGTGAAAATGAGGATAGGTAGAGAAACTGATAGAGCTTCTATGGCAACCAAGAACTGAACACCTTGCCACCAGTTCAGATTTGATTTTGAAAACATCCTAAAGTCTACACCATTTTATGAATTAATTTTCTTGTTTTTTTTGGGAGATTTTTGGAGATTTAATTGAATTTGTGTTTGCTATTTGGATTTGCTATTTTAGGAATGAGTGAAAGCACGTGAATTTTTTTTTCACGCAATCATGGAGGGAAGTGAAAACAGATATGAAAGGAGGAGAGAAACTAAGGAGATTCCATGGAGGGGAGAGAGAAAGAGAAGGGAGGAAAAAAACAATCCACCTAATTGAAAATTTAAAAAAATAGTACAAATGGCCACCTCATTTAAAATTTGCCCACTCAACACGCCATTTCACTTTTCTGTTAGTCAACTAACGGTCAAATTAACTCAGGGATCATTTAGAAGTATTTTTCAAACCTCAGGGACTAAAATGTTCATTTTGAAACTTCAAGGACCAAATAGACATCAAAGCTCAAACCTTGGGGACCAAAAGTGTATTTTGCCCATGTTTTATTTATTTAATATTTTCATCATCACATCGTTTCAAATCTCAATTTTTGTCCTAACATTCTATTTTTTTTTTTAATTTTAATTTAGCCAGTAAATGTATACAAATTTCATGAACCAACTTTGGTAGAAATATGAGTGTTGCTATGTACTTCGCTTTAAGTTCAAAAGTCCAAATCTTCATTCTTCACATTTGTTGTACTAAACTTTGGTAGAAACGGAACTTATAAGTTGTTATGTAAACATTCCCATACCACATTTATTGTAGAGTGACGATATACGCTTGCTATTTTGTCATGAGCCTAGCTCAGCTATAGTTGATATGTACTTCTCTTTAAACATGAAGGTTCAAATCTCCCTACCATACATTTGTTGTACTGAAACTCCAATAGAAACAAAACATATAAGCTTTTACATAAACATTTCCATACCACATTTATTGTAGAGTGACGATATACGCTTGATATTTGCAAAACTATTTGTTTTTTGCTTAAACTCTACCATATCCTGAAATAGCATTCAAACTTTATTTTCACAAACAGATTATTTGTTTTTTCTGTTTTATGCATCAGAGCTTCAAGGGATTGATCGTACGATATTAATGAGGGCATTGAAGCTACTTGAACAAAAGGGGAAGCTTGCAGTTTTCAAAGGTTCTTCAACAGATGATGAAGGCATTAAATTCTCTGTATAACTACCAATATGTATTCACATTGACTGCCACTCTTTGTTTTTTAACACTTCTAAATGGAACTTTCATTTAGTTTCCCCGTCAATTCAAACTGGGATTATATGTTTCTTGGTGCAGTTGTTCAGTGTAACGTAACCAAGAGGCCTCCCAATAGAACTTGAAGAGCCCATGCTTGGGAGTACTTTGGGAAATAGTTATTGTATATTGTTAAGAAAAACTAAAAACAATCTTTTTATTTACTTGAGTATTTGGACTGGTTTATGAGCTGGTGGATAGTCCAATGATTGTACGATATTTGATTTTTAAAATTCTTTTATAGAAAATTCTATATAGGTAGTCACTGTAGGCTTTTTAGATATTCGAAAAAAATCATTCAGGGAAAACTTCTTGTCCTTGAATTTTG

mRNA sequence

ATGCAGAAATTGGGAGATTTCAAGCTACCACATTTCTTCAATTATCCTCCATATTTCACTTTGCAACCAGTGCGGGACACCCGGGAGAAGCAAATTCAACTTTGGAAGGAACTTATCCTCGATTACTGCCGAACGCAAAAGATATTTGTCATTGGACTCGAAGAGGACTTTCCTCTATTTTCAAATTCTGAGATTGAAAGATCTCTCAGTCATGAAGCTAGAGAAGCTTTTCTCTCAGCTTTAGTGAAAAATAATGGACTTGAAGATAGTGTTATGACCATCGAGGAGATACGTTCAGGAGTTGAGTCTAGAGGAACAGAGCTTCAAGGGATTGATCGTACGATATTAATGAGGGCATTGAAGCTACTTGAACAAAAGGGGAAGCTTGCAGTTTTCAAAGGTTCTTCAACAGATGATGAAGGCATTAAATTCTCTGTATAACTACCAATATGTATTCACATTGACTGCCACTCTTTGTTTTTTAACACTTCTAAATGGAACTTTCATTTAGTTTCCCCGTCAATTCAAACTGGGATTATATGTTTCTTGGTGCAGTTGTTCAGTGTAACGTAACCAAGAGGCCTCCCAATAGAACTTGAAGAGCCCATGCTTGGGAGTACTTTGGGAAATAGTTATTGTATATTGTTAAGAAAAACTAAAAACAATCTTTTTATTTACTTGAGTATTTGGACTGGTTTATGAGCTGGTGGATAGTCCAATGATTGTACGATATTTGATTTTTAAAATTCTTTTATAGAAAATTCTATATAGGTAGTCACTGTAGGCTTTTTAGATATTCGAAAAAAATCATTCAGGGAAAACTTCTTGTCCTTGAATTTTG

Coding sequence (CDS)

ATGCAGAAATTGGGAGATTTCAAGCTACCACATTTCTTCAATTATCCTCCATATTTCACTTTGCAACCAGTGCGGGACACCCGGGAGAAGCAAATTCAACTTTGGAAGGAACTTATCCTCGATTACTGCCGAACGCAAAAGATATTTGTCATTGGACTCGAAGAGGACTTTCCTCTATTTTCAAATTCTGAGATTGAAAGATCTCTCAGTCATGAAGCTAGAGAAGCTTTTCTCTCAGCTTTAGTGAAAAATAATGGACTTGAAGATAGTGTTATGACCATCGAGGAGATACGTTCAGGAGTTGAGTCTAGAGGAACAGAGCTTCAAGGGATTGATCGTACGATATTAATGAGGGCATTGAAGCTACTTGAACAAAAGGGGAAGCTTGCAGTTTTCAAAGGTTCTTCAACAGATGATGAAGGCATTAAATTCTCTGTATAA

Protein sequence

MQKLGDFKLPHFFNYPPYFTLQPVRDTREKQIQLWKELILDYCRTQKIFVIGLEEDFPLFSNSEIERSLSHEAREAFLSALVKNNGLEDSVMTIEEIRSGVESRGTELQGIDRTILMRALKLLEQKGKLAVFKGSSTDDEGIKFSV

Homology

[Close]

BLAST of Lsi06G009200 vs. ExPASy Swiss-Prot
Match: Q8VZC9 (Vacuolar protein sorting-associated protein 25 OS=Arabidopsis thaliana OX=3702 GN=VPS25 PE=1 SV=1)

HSP 1 Score: 243.0 bits (619), Expect = 2.0e-63
Identity = 126/179 (70.39%), Postives = 138/179 (77.09%), Query Frame = 0


Query: 1   MQKLGDFKLPHFFNYPPYFTLQPVRDTREKQIQLWKELILDYCRTQKIFVIGLEEDFPLF 60
           MQKL DFKLP FFNYPPYFTLQPVRDTREKQIQLWKELILDYC++QKIF+IG+EEDFPLF
Sbjct: 1   MQKLADFKLPQFFNYPPYFTLQPVRDTREKQIQLWKELILDYCKSQKIFLIGVEEDFPLF 60

Query: 61  SNSEIERSLSHEAREAFLSAL---------------------------------VKNNGL 120
           SNS I+RSLSHEARE FLSA+                                 V++NGL
Sbjct: 61  SNSAIDRSLSHEARETFLSAIVGEGRAEWLDKGHRKCLILWHRIQDWADIVLQFVRDNGL 120

Query: 121 EDSVMTIEEIRSGVESRGTELQGIDRTILMRALKLLEQKGKLAVFKGSSTDDEGIKFSV 147
           EDSVMT+EEIRSG ES GTELQGIDRTILMRALKLLE KGKLA+FKG+S DDEG+KFSV
Sbjct: 121 EDSVMTVEEIRSGTESLGTELQGIDRTILMRALKLLENKGKLALFKGTSADDEGVKFSV 179

[Close]

BLAST of Lsi06G009200 vs. ExPASy Swiss-Prot
Match: Q6AX45 (Vacuolar protein-sorting-associated protein 25 OS=Xenopus laevis OX=8355 GN=vps25 PE=2 SV=1)

HSP 1 Score: 100.9 bits (250), Expect = 1.3e-20
Identity = 57/173 (32.95%), Postives = 90/173 (52.02%), Query Frame = 0


Query: 7   FKLPHFFNYPPYFTLQPVRDTREKQIQLWKELILDYCRTQKIFVIGLEE--DFPLFSNSE 66
           F+ P  +N+PP+FTLQP  DTR+KQ+  W  L+L YCR  K++ + L E  + PLF+N +
Sbjct: 3   FEWPWQYNFPPFFTLQPNVDTRQKQLSAWSSLVLSYCRQNKLYTMNLMEIQESPLFNNKK 62

Query: 67  IERSLSHEAREAFLSAL---------------------------------VKNNGLEDSV 126
           I+R LS E+ +  L  L                                 V  NG+ +SV
Sbjct: 63  IQRKLSLESVQVVLEELRKKGNLEWIDKNKSRFLIMWRRPDEWGKVIYQWVSKNGMTNSV 122

Query: 127 MTIEEIRSGVESRGTELQGIDRTILMRALKLLEQKGKLAVFKGSSTDDEGIKF 145
            T+ E+ SG ++ G E  G+D  +L+R+L+ L+Q+ K  +   +  +  G+KF
Sbjct: 123 FTLYELISGDDTEGEEFHGLDEAMLLRSLEALQQEHKAEII--TLNESRGVKF 173

[Close]

BLAST of Lsi06G009200 vs. ExPASy Swiss-Prot
Match: Q55GD9 (Vacuolar protein-sorting-associated protein 25 OS=Dictyostelium discoideum OX=44689 GN=vps25 PE=1 SV=1)

HSP 1 Score: 93.6 bits (231), Expect = 2.0e-18
Identity = 55/188 (29.26%), Postives = 96/188 (51.06%), Query Frame = 0


Query: 7   FKLPHFFNYPPYFTLQPVRDTREKQIQLWKELILDYCRTQKIFVIGLEEDFP----LFSN 66
           F+ P +++  P+FT+QP+ +TR+KQ Q+W++LIL YCR  KI+ + + E       LF+N
Sbjct: 6   FQFPPYYHKEPFFTIQPILNTRKKQFQMWQDLILQYCRYYKIYELDINESIKSNSVLFNN 65

Query: 67  SEIERSLSHEAREAFLSALVKNN------------------------------------- 126
            +I R LS EA ++ +  +++N                                      
Sbjct: 66  EKINRKLSREALKSIIDDIIENGFAEWVDKEKEKEKEKDKDNNNNNRVLIMWRKPDEWAS 125

Query: 127 ---------GLEDSVMTIEEIRSGVESRGTELQGIDRTILMRALKLLEQKGKLAVFKGSS 145
                    GL ++V+T+ EI++G +S+  E   ++ TILM++LK+LE++ K   F  S 
Sbjct: 126 LIYKWVADCGLLNTVLTVWEIQNGDDSKKQEFHQLNTTILMKSLKVLEKQSKCQTF--SQ 185

[Close]

BLAST of Lsi06G009200 vs. ExPASy Swiss-Prot
Match: Q6NWF4 (Vacuolar protein-sorting-associated protein 25 OS=Danio rerio OX=7955 GN=vps25 PE=2 SV=2)

HSP 1 Score: 92.0 bits (227), Expect = 5.8e-18
Identity = 54/173 (31.21%), Postives = 89/173 (51.45%), Query Frame = 0


Query: 7   FKLPHFFNYPPYFTLQPVRDTREKQIQLWKELILDYCRTQKIFVIGLEE--DFPLFSNSE 66
           F+ P  +N+PP+FTLQP  DTR+KQ+  W  L+L YCR +K++ + + E  + P+F+N +
Sbjct: 3   FEWPWQYNFPPFFTLQPNVDTRQKQLAAWCSLVLSYCRHRKLYTLDVLEAQESPVFNNKK 62

Query: 67  IERSLSHEAREAFLSAL---------------------------------VKNNGLEDSV 126
           IER LS EA +     L                                 V  NG+ +SV
Sbjct: 63  IERKLSVEAIQVVFEELRKKGNLEWLDKNKSRCLIMWRRPEEWGKLIYQWVSKNGMVNSV 122

Query: 127 MTIEEIRSGVESRGTELQGIDRTILMRALKLLEQKGKLAVFKGSSTDDEGIKF 145
            T+ E+ +G ++   E  G++  +L+R+L+ L+  GK  +   +  D +G+KF
Sbjct: 123 FTLYELANGDDTEKEEFHGLEDWMLLRSLQALQTDGKAEII--TMDDGKGVKF 173

[Close]

BLAST of Lsi06G009200 vs. ExPASy Swiss-Prot
Match: Q9CQ80 (Vacuolar protein-sorting-associated protein 25 OS=Mus musculus OX=10090 GN=Vps25 PE=1 SV=1)

HSP 1 Score: 83.2 bits (204), Expect = 2.7e-15
Identity = 52/173 (30.06%), Postives = 83/173 (47.98%), Query Frame = 0


Query: 7   FKLPHFFNYPPYFTLQPVRDTREKQIQLWKELILDYCR--TQKIFVIGLEEDFPLFSNSE 66
           F+ P  + +PP+FTLQP  DTR+KQ+  W  L+L +CR   Q    +   ++ PLF+N +
Sbjct: 5   FEWPWQYRFPPFFTLQPNVDTRQKQLAAWCSLVLSFCRLHKQSSMTVMEAQESPLFNNVK 64

Query: 67  IERSLSHEAREAFLSALVKNNGLE---------------------------------DSV 126
           ++R L  E+ +  L  L K   LE                                 +SV
Sbjct: 65  LQRKLPVESIQIVLEELRKKGNLEWLDKNKSSFLIMWRRPEEWGKLIYQWVSRSGQNNSV 124

Query: 127 MTIEEIRSGVESRGTELQGIDRTILMRALKLLEQKGKLAVFKGSSTDDEGIKF 145
            T+ E+ SG ++   E  G+D   L+RAL+ L+Q+ K  +   + +D  G+KF
Sbjct: 125 FTLYELTSGEDTEDEEFHGLDEATLLRALQALQQEHKAEII--TVSDGRGVKF 175

[Close]

BLAST of Lsi06G009200 vs. ExPASy TrEMBL
Match: A0A5A7UHJ5 (Vacuolar protein sorting-associated protein 25 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold139G001240 PE=3 SV=1)

HSP 1 Score: 268.9 bits (686), Expect = 1.3e-68
Identity = 142/179 (79.33%), Postives = 145/179 (81.01%), Query Frame = 0


Query: 1   MQKLGDFKLPHFFNYPPYFTLQPVRDTREKQIQLWKELILDYCRTQKIFVIGLEEDFPLF 60
           MQKLGDFKLPHFFNYPPYFTLQPVRDTREKQIQLWKELILDYCRTQKIFVIGLEEDFPLF
Sbjct: 1   MQKLGDFKLPHFFNYPPYFTLQPVRDTREKQIQLWKELILDYCRTQKIFVIGLEEDFPLF 60

Query: 61  SNSEIERSLSHEAREAFLSAL---------------------------------VKNNGL 120
           SNS IERSLSHEAR+AFLSAL                                 VKNNGL
Sbjct: 61  SNSVIERSLSHEARQAFLSALVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGL 120

Query: 121 EDSVMTIEEIRSGVESRGTELQGIDRTILMRALKLLEQKGKLAVFKGSSTDDEGIKFSV 147
           EDSVMT+EEIRSGVESRGTELQGIDRTILMRALKLLEQKGKLA+FKGSSTDDEGIKFSV
Sbjct: 121 EDSVMTVEEIRSGVESRGTELQGIDRTILMRALKLLEQKGKLAIFKGSSTDDEGIKFSV 179

[Close]

BLAST of Lsi06G009200 vs. ExPASy TrEMBL
Match: A0A1S3BKR8 (vacuolar protein sorting-associated protein 25 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103490745 PE=3 SV=1)

HSP 1 Score: 268.9 bits (686), Expect = 1.3e-68
Identity = 142/179 (79.33%), Postives = 145/179 (81.01%), Query Frame = 0


Query: 1   MQKLGDFKLPHFFNYPPYFTLQPVRDTREKQIQLWKELILDYCRTQKIFVIGLEEDFPLF 60
           MQKLGDFKLPHFFNYPPYFTLQPVRDTREKQIQLWKELILDYCRTQKIFVIGLEEDFPLF
Sbjct: 1   MQKLGDFKLPHFFNYPPYFTLQPVRDTREKQIQLWKELILDYCRTQKIFVIGLEEDFPLF 60

Query: 61  SNSEIERSLSHEAREAFLSAL---------------------------------VKNNGL 120
           SNS IERSLSHEAR+AFLSAL                                 VKNNGL
Sbjct: 61  SNSVIERSLSHEARQAFLSALVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGL 120

Query: 121 EDSVMTIEEIRSGVESRGTELQGIDRTILMRALKLLEQKGKLAVFKGSSTDDEGIKFSV 147
           EDSVMT+EEIRSGVESRGTELQGIDRTILMRALKLLEQKGKLA+FKGSSTDDEGIKFSV
Sbjct: 121 EDSVMTVEEIRSGVESRGTELQGIDRTILMRALKLLEQKGKLAIFKGSSTDDEGIKFSV 179

[Close]

BLAST of Lsi06G009200 vs. ExPASy TrEMBL
Match: A0A0A0L1R7 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G006740 PE=3 SV=1)

HSP 1 Score: 266.9 bits (681), Expect = 4.9e-68
Identity = 140/179 (78.21%), Postives = 145/179 (81.01%), Query Frame = 0


Query: 1   MQKLGDFKLPHFFNYPPYFTLQPVRDTREKQIQLWKELILDYCRTQKIFVIGLEEDFPLF 60
           MQKLGDFKLPHFFNYPPYFTLQPVRDTREKQIQLWKELILDYCRTQKIFVIGLEEDFPLF
Sbjct: 1   MQKLGDFKLPHFFNYPPYFTLQPVRDTREKQIQLWKELILDYCRTQKIFVIGLEEDFPLF 60

Query: 61  SNSEIERSLSHEAREAFLSAL---------------------------------VKNNGL 120
           SNS IERSLSHEAR+AF+SAL                                 VKNNGL
Sbjct: 61  SNSVIERSLSHEARQAFISALVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGL 120

Query: 121 EDSVMTIEEIRSGVESRGTELQGIDRTILMRALKLLEQKGKLAVFKGSSTDDEGIKFSV 147
           EDSVMT+EEIRSGVESRGTEL+GIDRTILMRALKLLEQKGKLA+FKGSSTDDEGIKFSV
Sbjct: 121 EDSVMTVEEIRSGVESRGTELEGIDRTILMRALKLLEQKGKLAIFKGSSTDDEGIKFSV 179

[Close]

BLAST of Lsi06G009200 vs. ExPASy TrEMBL
Match: A0A6J1CUP8 (vacuolar protein sorting-associated protein 25 OS=Momordica charantia OX=3673 GN=LOC111014756 PE=3 SV=1)

HSP 1 Score: 264.6 bits (675), Expect = 2.4e-67
Identity = 138/179 (77.09%), Postives = 145/179 (81.01%), Query Frame = 0


Query: 1   MQKLGDFKLPHFFNYPPYFTLQPVRDTREKQIQLWKELILDYCRTQKIFVIGLEEDFPLF 60
           MQKLGDFKLPHFFNYPPYFTLQPVRDTREKQIQLWKELILDYCRTQKIFVIGLEE+FP+F
Sbjct: 1   MQKLGDFKLPHFFNYPPYFTLQPVRDTREKQIQLWKELILDYCRTQKIFVIGLEEEFPIF 60

Query: 61  SNSEIERSLSHEAREAFLSAL---------------------------------VKNNGL 120
           +N+ IERSLSHEAREAFLSAL                                 VKNNGL
Sbjct: 61  TNTAIERSLSHEAREAFLSALVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGL 120

Query: 121 EDSVMTIEEIRSGVESRGTELQGIDRTILMRALKLLEQKGKLAVFKGSSTDDEGIKFSV 147
           EDSVMT+EE+RSGVESRGTELQGIDRTILMRALKLLEQKGKLA+FKGSSTDDEGIKFSV
Sbjct: 121 EDSVMTVEEMRSGVESRGTELQGIDRTILMRALKLLEQKGKLAIFKGSSTDDEGIKFSV 179

[Close]

BLAST of Lsi06G009200 vs. ExPASy TrEMBL
Match: A0A6J1L2J9 (vacuolar protein sorting-associated protein 25 OS=Cucurbita maxima OX=3661 GN=LOC111498518 PE=3 SV=1)

HSP 1 Score: 262.3 bits (669), Expect = 1.2e-66
Identity = 139/179 (77.65%), Postives = 143/179 (79.89%), Query Frame = 0


Query: 1   MQKLGDFKLPHFFNYPPYFTLQPVRDTREKQIQLWKELILDYCRTQKIFVIGLEEDFPLF 60
           MQKLGDFKLPHFFNYPPYFTLQPV+DTREKQIQLWKELILDYCRTQKIFVIGLEE+FPLF
Sbjct: 1   MQKLGDFKLPHFFNYPPYFTLQPVKDTREKQIQLWKELILDYCRTQKIFVIGLEEEFPLF 60

Query: 61  SNSEIERSLSHEAREAFLSAL---------------------------------VKNNGL 120
           SNS IERSLSHEAR AFLSAL                                 VKNNGL
Sbjct: 61  SNSVIERSLSHEARVAFLSALVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGL 120

Query: 121 EDSVMTIEEIRSGVESRGTELQGIDRTILMRALKLLEQKGKLAVFKGSSTDDEGIKFSV 147
           EDSVMT+EEIRSGVESRGTELQGIDRTILMRALK LEQKGKLA+FKGSSTDDEGIKFSV
Sbjct: 121 EDSVMTVEEIRSGVESRGTELQGIDRTILMRALKQLEQKGKLAIFKGSSTDDEGIKFSV 179

[Close]

BLAST of Lsi06G009200 vs. NCBI nr
Match: XP_038891553.1 (vacuolar protein sorting-associated protein 25 [Benincasa hispida])

HSP 1 Score: 272.7 bits (696), Expect = 1.8e-69
Identity = 144/179 (80.45%), Postives = 146/179 (81.56%), Query Frame = 0


Query: 1   MQKLGDFKLPHFFNYPPYFTLQPVRDTREKQIQLWKELILDYCRTQKIFVIGLEEDFPLF 60
           MQKLGDFKLPHFFNYPPYFTLQPVRDTREKQIQLWKELILDYCRTQKIFVIGLEEDFPLF
Sbjct: 1   MQKLGDFKLPHFFNYPPYFTLQPVRDTREKQIQLWKELILDYCRTQKIFVIGLEEDFPLF 60

Query: 61  SNSEIERSLSHEAREAFLSAL---------------------------------VKNNGL 120
           SNSEIERSLSHEAREAFLSAL                                 VKNNGL
Sbjct: 61  SNSEIERSLSHEAREAFLSALVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGL 120

Query: 121 EDSVMTIEEIRSGVESRGTELQGIDRTILMRALKLLEQKGKLAVFKGSSTDDEGIKFSV 147
           EDSVMTIEEIRSGVESRGTELQGIDRT+LMRALKLLEQKGKLA+FKGSSTDDEGIKFSV
Sbjct: 121 EDSVMTIEEIRSGVESRGTELQGIDRTVLMRALKLLEQKGKLAIFKGSSTDDEGIKFSV 179

[Close]

BLAST of Lsi06G009200 vs. NCBI nr
Match: XP_008448627.1 (PREDICTED: vacuolar protein sorting-associated protein 25 isoform X1 [Cucumis melo] >KAA0053011.1 vacuolar protein sorting-associated protein 25 isoform X1 [Cucumis melo var. makuwa] >TYK11467.1 vacuolar protein sorting-associated protein 25 isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 268.9 bits (686), Expect = 2.6e-68
Identity = 142/179 (79.33%), Postives = 145/179 (81.01%), Query Frame = 0


Query: 1   MQKLGDFKLPHFFNYPPYFTLQPVRDTREKQIQLWKELILDYCRTQKIFVIGLEEDFPLF 60
           MQKLGDFKLPHFFNYPPYFTLQPVRDTREKQIQLWKELILDYCRTQKIFVIGLEEDFPLF
Sbjct: 1   MQKLGDFKLPHFFNYPPYFTLQPVRDTREKQIQLWKELILDYCRTQKIFVIGLEEDFPLF 60

Query: 61  SNSEIERSLSHEAREAFLSAL---------------------------------VKNNGL 120
           SNS IERSLSHEAR+AFLSAL                                 VKNNGL
Sbjct: 61  SNSVIERSLSHEARQAFLSALVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGL 120

Query: 121 EDSVMTIEEIRSGVESRGTELQGIDRTILMRALKLLEQKGKLAVFKGSSTDDEGIKFSV 147
           EDSVMT+EEIRSGVESRGTELQGIDRTILMRALKLLEQKGKLA+FKGSSTDDEGIKFSV
Sbjct: 121 EDSVMTVEEIRSGVESRGTELQGIDRTILMRALKLLEQKGKLAIFKGSSTDDEGIKFSV 179

[Close]

BLAST of Lsi06G009200 vs. NCBI nr
Match: XP_004146105.1 (vacuolar protein sorting-associated protein 25 [Cucumis sativus] >KGN55713.1 hypothetical protein Csa_009640 [Cucumis sativus])

HSP 1 Score: 266.9 bits (681), Expect = 1.0e-67
Identity = 140/179 (78.21%), Postives = 145/179 (81.01%), Query Frame = 0


Query: 1   MQKLGDFKLPHFFNYPPYFTLQPVRDTREKQIQLWKELILDYCRTQKIFVIGLEEDFPLF 60
           MQKLGDFKLPHFFNYPPYFTLQPVRDTREKQIQLWKELILDYCRTQKIFVIGLEEDFPLF
Sbjct: 1   MQKLGDFKLPHFFNYPPYFTLQPVRDTREKQIQLWKELILDYCRTQKIFVIGLEEDFPLF 60

Query: 61  SNSEIERSLSHEAREAFLSAL---------------------------------VKNNGL 120
           SNS IERSLSHEAR+AF+SAL                                 VKNNGL
Sbjct: 61  SNSVIERSLSHEARQAFISALVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGL 120

Query: 121 EDSVMTIEEIRSGVESRGTELQGIDRTILMRALKLLEQKGKLAVFKGSSTDDEGIKFSV 147
           EDSVMT+EEIRSGVESRGTEL+GIDRTILMRALKLLEQKGKLA+FKGSSTDDEGIKFSV
Sbjct: 121 EDSVMTVEEIRSGVESRGTELEGIDRTILMRALKLLEQKGKLAIFKGSSTDDEGIKFSV 179

[Close]

BLAST of Lsi06G009200 vs. NCBI nr
Match: XP_022145259.1 (vacuolar protein sorting-associated protein 25 [Momordica charantia])

HSP 1 Score: 264.6 bits (675), Expect = 5.0e-67
Identity = 138/179 (77.09%), Postives = 145/179 (81.01%), Query Frame = 0


Query: 1   MQKLGDFKLPHFFNYPPYFTLQPVRDTREKQIQLWKELILDYCRTQKIFVIGLEEDFPLF 60
           MQKLGDFKLPHFFNYPPYFTLQPVRDTREKQIQLWKELILDYCRTQKIFVIGLEE+FP+F
Sbjct: 1   MQKLGDFKLPHFFNYPPYFTLQPVRDTREKQIQLWKELILDYCRTQKIFVIGLEEEFPIF 60

Query: 61  SNSEIERSLSHEAREAFLSAL---------------------------------VKNNGL 120
           +N+ IERSLSHEAREAFLSAL                                 VKNNGL
Sbjct: 61  TNTAIERSLSHEAREAFLSALVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGL 120

Query: 121 EDSVMTIEEIRSGVESRGTELQGIDRTILMRALKLLEQKGKLAVFKGSSTDDEGIKFSV 147
           EDSVMT+EE+RSGVESRGTELQGIDRTILMRALKLLEQKGKLA+FKGSSTDDEGIKFSV
Sbjct: 121 EDSVMTVEEMRSGVESRGTELQGIDRTILMRALKLLEQKGKLAIFKGSSTDDEGIKFSV 179

[Close]

BLAST of Lsi06G009200 vs. NCBI nr
Match: XP_022949655.1 (vacuolar protein sorting-associated protein 25 [Cucurbita moschata] >XP_023005573.1 vacuolar protein sorting-associated protein 25 [Cucurbita maxima] >KAG6596709.1 Vacuolar protein sorting-associated protein 25, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 262.3 bits (669), Expect = 2.5e-66
Identity = 139/179 (77.65%), Postives = 143/179 (79.89%), Query Frame = 0


Query: 1   MQKLGDFKLPHFFNYPPYFTLQPVRDTREKQIQLWKELILDYCRTQKIFVIGLEEDFPLF 60
           MQKLGDFKLPHFFNYPPYFTLQPV+DTREKQIQLWKELILDYCRTQKIFVIGLEE+FPLF
Sbjct: 1   MQKLGDFKLPHFFNYPPYFTLQPVKDTREKQIQLWKELILDYCRTQKIFVIGLEEEFPLF 60

Query: 61  SNSEIERSLSHEAREAFLSAL---------------------------------VKNNGL 120
           SNS IERSLSHEAR AFLSAL                                 VKNNGL
Sbjct: 61  SNSVIERSLSHEARVAFLSALVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGL 120

Query: 121 EDSVMTIEEIRSGVESRGTELQGIDRTILMRALKLLEQKGKLAVFKGSSTDDEGIKFSV 147
           EDSVMT+EEIRSGVESRGTELQGIDRTILMRALK LEQKGKLA+FKGSSTDDEGIKFSV
Sbjct: 121 EDSVMTVEEIRSGVESRGTELQGIDRTILMRALKQLEQKGKLAIFKGSSTDDEGIKFSV 179

[Close]

BLAST of Lsi06G009200 vs. TAIR 10
Match: AT4G19003.2 (E2F/DP family winged-helix DNA-binding domain )

HSP 1 Score: 243.0 bits (619), Expect = 1.4e-64
Identity = 126/179 (70.39%), Postives = 138/179 (77.09%), Query Frame = 0


Query: 1   MQKLGDFKLPHFFNYPPYFTLQPVRDTREKQIQLWKELILDYCRTQKIFVIGLEEDFPLF 60
           MQKL DFKLP FFNYPPYFTLQPVRDTREKQIQLWKELILDYC++QKIF+IG+EEDFPLF
Sbjct: 1   MQKLADFKLPQFFNYPPYFTLQPVRDTREKQIQLWKELILDYCKSQKIFLIGVEEDFPLF 60

Query: 61  SNSEIERSLSHEAREAFLSAL---------------------------------VKNNGL 120
           SNS I+RSLSHEARE FLSA+                                 V++NGL
Sbjct: 61  SNSAIDRSLSHEARETFLSAIVGEGRAEWLDKGHRKCLILWHRIQDWADIVLQFVRDNGL 120

Query: 121 EDSVMTIEEIRSGVESRGTELQGIDRTILMRALKLLEQKGKLAVFKGSSTDDEGIKFSV 147
           EDSVMT+EEIRSG ES GTELQGIDRTILMRALKLLE KGKLA+FKG+S DDEG+KFSV
Sbjct: 121 EDSVMTVEEIRSGTESLGTELQGIDRTILMRALKLLENKGKLALFKGTSADDEGVKFSV 179

[Close]

BLAST of Lsi06G009200 vs. TAIR 10
Match: AT4G19003.1 (E2F/DP family winged-helix DNA-binding domain )

HSP 1 Score: 243.0 bits (619), Expect = 1.4e-64
Identity = 126/179 (70.39%), Postives = 138/179 (77.09%), Query Frame = 0


Query: 1   MQKLGDFKLPHFFNYPPYFTLQPVRDTREKQIQLWKELILDYCRTQKIFVIGLEEDFPLF 60
           MQKL DFKLP FFNYPPYFTLQPVRDTREKQIQLWKELILDYC++QKIF+IG+EEDFPLF
Sbjct: 1   MQKLADFKLPQFFNYPPYFTLQPVRDTREKQIQLWKELILDYCKSQKIFLIGVEEDFPLF 60

Query: 61  SNSEIERSLSHEAREAFLSAL---------------------------------VKNNGL 120
           SNS I+RSLSHEARE FLSA+                                 V++NGL
Sbjct: 61  SNSAIDRSLSHEARETFLSAIVGEGRAEWLDKGHRKCLILWHRIQDWADIVLQFVRDNGL 120

Query: 121 EDSVMTIEEIRSGVESRGTELQGIDRTILMRALKLLEQKGKLAVFKGSSTDDEGIKFSV 147
           EDSVMT+EEIRSG ES GTELQGIDRTILMRALKLLE KGKLA+FKG+S DDEG+KFSV
Sbjct: 121 EDSVMTVEEIRSGTESLGTELQGIDRTILMRALKLLENKGKLALFKGTSADDEGVKFSV 179

The following BLAST results are available for this feature:

BLAST of Lsi06G009200 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8VZC9	2.0e-63	70.39	Vacuolar protein sorting-associated protein 25 OS=Arabidopsis thaliana OX=3702 G...	[more]
Q6AX45	1.3e-20	32.95	Vacuolar protein-sorting-associated protein 25 OS=Xenopus laevis OX=8355 GN=vps2...	[more]
Q55GD9	2.0e-18	29.26	Vacuolar protein-sorting-associated protein 25 OS=Dictyostelium discoideum OX=44...	[more]
Q6NWF4	5.8e-18	31.21	Vacuolar protein-sorting-associated protein 25 OS=Danio rerio OX=7955 GN=vps25 P...	[more]
Q9CQ80	2.7e-15	30.06	Vacuolar protein-sorting-associated protein 25 OS=Mus musculus OX=10090 GN=Vps25...	[more]

BLAST of Lsi06G009200 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7UHJ5	1.3e-68	79.33	Vacuolar protein sorting-associated protein 25 isoform X1 OS=Cucumis melo var. m...	[more]
A0A1S3BKR8	1.3e-68	79.33	vacuolar protein sorting-associated protein 25 isoform X1 OS=Cucumis melo OX=365...	[more]
A0A0A0L1R7	4.9e-68	78.21	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G006740 PE=3 SV=1	[more]
A0A6J1CUP8	2.4e-67	77.09	vacuolar protein sorting-associated protein 25 OS=Momordica charantia OX=3673 GN...	[more]
A0A6J1L2J9	1.2e-66	77.65	vacuolar protein sorting-associated protein 25 OS=Cucurbita maxima OX=3661 GN=LO...	[more]

BLAST of Lsi06G009200 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038891553.1	1.8e-69	80.45	vacuolar protein sorting-associated protein 25 [Benincasa hispida]	[more]
XP_008448627.1	2.6e-68	79.33	PREDICTED: vacuolar protein sorting-associated protein 25 isoform X1 [Cucumis me...	[more]
XP_004146105.1	1.0e-67	78.21	vacuolar protein sorting-associated protein 25 [Cucumis sativus] >KGN55713.1 hyp...	[more]
XP_022145259.1	5.0e-67	77.09	vacuolar protein sorting-associated protein 25 [Momordica charantia]	[more]
XP_022949655.1	2.5e-66	77.65	vacuolar protein sorting-associated protein 25 [Cucurbita moschata] >XP_02300557...	[more]

BLAST of Lsi06G009200 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G19003.2	1.4e-64	70.39	E2F/DP family winged-helix DNA-binding domain	[more]
AT4G19003.1	1.4e-64	70.39	E2F/DP family winged-helix DNA-binding domain	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR008570	ESCRT-II complex, Vps25 subunit	PFAM	PF05871	ESCRT-II	coord: 13..85 e-value: 2.6E-21 score: 76.3
IPR008570	ESCRT-II complex, Vps25 subunit	PANTHER	PTHR13149	VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN VPS25	coord: 1..84 coord: 81..146
IPR014041	ESCRT-II complex, Vps25 subunit, N-terminal winged helix	GENE3D	1.10.10.570		coord: 4..97 e-value: 2.4E-25 score: 90.7
None	No IPR available	PANTHER	PTHR13149:SF1	SUBFAMILY NOT NAMED	coord: 1..84 coord: 81..146
IPR036390	Winged helix DNA-binding domain superfamily	SUPERFAMILY	46785	"Winged helix" DNA-binding domain	coord: 80..144
IPR036390	Winged helix DNA-binding domain superfamily	SUPERFAMILY	46785	"Winged helix" DNA-binding domain	coord: 7..88

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi06G009200.1	Lsi06G009200.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0043328	protein transport to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway
biological_process	GO:0071985	multivesicular body sorting pathway
cellular_component	GO:0000814	ESCRT II complex
molecular_function	GO:0008168	methyltransferase activity
molecular_function	GO:0042803	protein homodimerization activity
molecular_function	GO:0005198	structural molecule activity