Lsi06G008290 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi06G008290
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionE3 ubiquitin-protein ligase ATL42-like
Locationchr06: 17612827 .. 17614095 (-)
RNA-Seq ExpressionLsi06G008290
SyntenyLsi06G008290
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAGTAAGTTTAGTAGAATCATTATGATAGTTATGACTATTTTGCTTTTTCCTGTCAATGCACAAGCACCTCCTCCACCAAAAGCTGGTTCTGGTATTCCACAAAACTTCACCATTGTTATTGGAATCCTCTCCATCATGTTCTCTATGGCCTTCATCCTTGTTCTTTATGCAAAATTCTGTCACCAGACTTCCCAACTTCGTGGTGAACCACAAGCTCATCTCGGGCTTGGACTTCTCAGATCAGACTCGGGATTTTCTGGGGTTGATCAAAAGGTGATTGATGCACTTCCTTTCTTTAAATTCTCATCACTCAAAGGATCAAGGGAAGGGCTGGAATGTGCTGTTTGCTTATCCAAATTTGAGGACATTGAAGTCCTTCGGTTGCTTCCGAAATGCAAGCATGCCTTTCACATTGGCTGTATTGATCATTGGCTCGAAAAGCACTCGAGCTGTCCGCTTTGTCGGTGTAGGATCAGCATTGAGGACGTTGCATTCTTTACCTATTCCAACAGTATGAGACTAATGAGGAACAACAGCCAGCCAGAGCTGCCACAAGATTCACACATGGAGCTCTTCGTACAGAGAGAGGAAGATCATCATAACTCTTCAAGGTTCAACACTGGATCCAGCTTCAGAAAAACAGGAAAGGGTAACAAGGAAAAAGAAGTCTTGATCGAAAACGAAGCCGGTGAACCTGGAAGTGAGGAGCGGGTGCTCTTTCACAAGCACAAACACAAGATTCTTGTATCTGACTTTGTTTTCATGAACAGATGGAGCAACATGAGTTCTTCTGACCTCATGTTCTTGAACACAGAAATGCTTGGGGCCATGTCTAGCAATAGATTCTCTTCTTTGAATTCAGAAAGCATTCGTCGATCGGCAACAAGAGAAACTGAGGGCAAAAGGACTTCACAAATTAAAGAAGAGATGGAAACGAAGAGGTCATTTGAGAGGAGGATAAGCATTGCTGAGAAAAATAGCTTAGTTTCAATCTCAGGTATTCCCTCTATATCAGATTCTAATGCAAGTACATCAAGGACCACAAATCTTAATGAAAGAAGATCTGTGTCAGACACCACTGCTTTCTCAAGATTTGGTCATTTCAATGAGAATGCAATAAGAGACAGTTCTATGATTGAAGCTGATGCAAAAGATTACAGAATTAGGCAGGTATGGTTGCCAATAGCCAAAAGAACAGTACAATGGTTTGCCAATAGAGAACAAAGGTCTCAAGTGTCAAAGTACACACAGCAGCCAGATGTATAG

mRNA sequence

ATGAGTAAGTTTAGTAGAATCATTATGATAGTTATGACTATTTTGCTTTTTCCTGTCAATGCACAAGCACCTCCTCCACCAAAAGCTGGTTCTGGTATTCCACAAAACTTCACCATTGTTATTGGAATCCTCTCCATCATGTTCTCTATGGCCTTCATCCTTGTTCTTTATGCAAAATTCTGTCACCAGACTTCCCAACTTCGTGGTGAACCACAAGCTCATCTCGGGCTTGGACTTCTCAGATCAGACTCGGGATTTTCTGGGGTTGATCAAAAGGTGATTGATGCACTTCCTTTCTTTAAATTCTCATCACTCAAAGGATCAAGGGAAGGGCTGGAATGTGCTGTTTGCTTATCCAAATTTGAGGACATTGAAGTCCTTCGGTTGCTTCCGAAATGCAAGCATGCCTTTCACATTGGCTGTATTGATCATTGGCTCGAAAAGCACTCGAGCTGTCCGCTTTGTCGGTGTAGGATCAGCATTGAGGACGTTGCATTCTTTACCTATTCCAACAGTATGAGACTAATGAGGAACAACAGCCAGCCAGAGCTGCCACAAGATTCACACATGGAGCTCTTCGTACAGAGAGAGGAAGATCATCATAACTCTTCAAGGTTCAACACTGGATCCAGCTTCAGAAAAACAGGAAAGGGTAACAAGGAAAAAGAAGTCTTGATCGAAAACGAAGCCGGTGAACCTGGAAGTGAGGAGCGGGTGCTCTTTCACAAGCACAAACACAAGATTCTTGTATCTGACTTTGTTTTCATGAACAGATGGAGCAACATGAGTTCTTCTGACCTCATGTTCTTGAACACAGAAATGCTTGGGGCCATGTCTAGCAATAGATTCTCTTCTTTGAATTCAGAAAGCATTCGTCGATCGGCAACAAGAGAAACTGAGGGCAAAAGGACTTCACAAATTAAAGAAGAGATGGAAACGAAGAGGTCATTTGAGAGGAGGATAAGCATTGCTGAGAAAAATAGCTTAGTTTCAATCTCAGGTATTCCCTCTATATCAGATTCTAATGCAAGTACATCAAGGACCACAAATCTTAATGAAAGAAGATCTGTGTCAGACACCACTGCTTTCTCAAGATTTGGTCATTTCAATGAGAATGCAATAAGAGACAGTTCTATGATTGAAGCTGATGCAAAAGATTACAGAATTAGGCAGGTATGGTTGCCAATAGCCAAAAGAACAGTACAATGGTTTGCCAATAGAGAACAAAGGTCTCAAGTGTCAAAGTACACACAGCAGCCAGATGTATAG

Coding sequence (CDS)

ATGAGTAAGTTTAGTAGAATCATTATGATAGTTATGACTATTTTGCTTTTTCCTGTCAATGCACAAGCACCTCCTCCACCAAAAGCTGGTTCTGGTATTCCACAAAACTTCACCATTGTTATTGGAATCCTCTCCATCATGTTCTCTATGGCCTTCATCCTTGTTCTTTATGCAAAATTCTGTCACCAGACTTCCCAACTTCGTGGTGAACCACAAGCTCATCTCGGGCTTGGACTTCTCAGATCAGACTCGGGATTTTCTGGGGTTGATCAAAAGGTGATTGATGCACTTCCTTTCTTTAAATTCTCATCACTCAAAGGATCAAGGGAAGGGCTGGAATGTGCTGTTTGCTTATCCAAATTTGAGGACATTGAAGTCCTTCGGTTGCTTCCGAAATGCAAGCATGCCTTTCACATTGGCTGTATTGATCATTGGCTCGAAAAGCACTCGAGCTGTCCGCTTTGTCGGTGTAGGATCAGCATTGAGGACGTTGCATTCTTTACCTATTCCAACAGTATGAGACTAATGAGGAACAACAGCCAGCCAGAGCTGCCACAAGATTCACACATGGAGCTCTTCGTACAGAGAGAGGAAGATCATCATAACTCTTCAAGGTTCAACACTGGATCCAGCTTCAGAAAAACAGGAAAGGGTAACAAGGAAAAAGAAGTCTTGATCGAAAACGAAGCCGGTGAACCTGGAAGTGAGGAGCGGGTGCTCTTTCACAAGCACAAACACAAGATTCTTGTATCTGACTTTGTTTTCATGAACAGATGGAGCAACATGAGTTCTTCTGACCTCATGTTCTTGAACACAGAAATGCTTGGGGCCATGTCTAGCAATAGATTCTCTTCTTTGAATTCAGAAAGCATTCGTCGATCGGCAACAAGAGAAACTGAGGGCAAAAGGACTTCACAAATTAAAGAAGAGATGGAAACGAAGAGGTCATTTGAGAGGAGGATAAGCATTGCTGAGAAAAATAGCTTAGTTTCAATCTCAGGTATTCCCTCTATATCAGATTCTAATGCAAGTACATCAAGGACCACAAATCTTAATGAAAGAAGATCTGTGTCAGACACCACTGCTTTCTCAAGATTTGGTCATTTCAATGAGAATGCAATAAGAGACAGTTCTATGATTGAAGCTGATGCAAAAGATTACAGAATTAGGCAGGTATGGTTGCCAATAGCCAAAAGAACAGTACAATGGTTTGCCAATAGAGAACAAAGGTCTCAAGTGTCAAAGTACACACAGCAGCCAGATGTATAG

Protein sequence

MSKFSRIIMIVMTILLFPVNAQAPPPPKAGSGIPQNFTIVIGILSIMFSMAFILVLYAKFCHQTSQLRGEPQAHLGLGLLRSDSGFSGVDQKVIDALPFFKFSSLKGSREGLECAVCLSKFEDIEVLRLLPKCKHAFHIGCIDHWLEKHSSCPLCRCRISIEDVAFFTYSNSMRLMRNNSQPELPQDSHMELFVQREEDHHNSSRFNTGSSFRKTGKGNKEKEVLIENEAGEPGSEERVLFHKHKHKILVSDFVFMNRWSNMSSSDLMFLNTEMLGAMSSNRFSSLNSESIRRSATRETEGKRTSQIKEEMETKRSFERRISIAEKNSLVSISGIPSISDSNASTSRTTNLNERRSVSDTTAFSRFGHFNENAIRDSSMIEADAKDYRIRQVWLPIAKRTVQWFANREQRSQVSKYTQQPDV
Homology
BLAST of Lsi06G008290 vs. ExPASy Swiss-Prot
Match: Q5XF85 (E3 ubiquitin-protein ligase ATL42 OS=Arabidopsis thaliana OX=3702 GN=ATL42 PE=1 SV=2)

HSP 1 Score: 303.5 bits (776), Expect = 3.7e-81
Identity = 192/447 (42.95%), Postives = 282/447 (63.09%), Query Frame = 0

Query: 6   RIIMIVMTILLFPVNAQAPPPPKAG---SGIPQNFTIVIGILSIMFSMAFILVLYAKFCH 65
           +I    + +L    +AQ PPP + G   +    +  +V G+L+IMF++ F+L++YAK CH
Sbjct: 3   QIFFFFLPLLHSYASAQTPPPFRNGDLVANFEPSLAVVTGVLAIMFALTFVLLVYAKCCH 62

Query: 66  ------QTSQLRGEPQAHLGLGLLRSDSG---FSGVDQKVIDALPFFKFSSLKGSREGLE 125
                    + R + +   G+   RS +    FSG+D+  I++LP F+FS+LKGS++GL+
Sbjct: 63  IDLRSGSGDRRRHDRRLRQGIFFNRSTASSDRFSGLDKTAIESLPLFRFSALKGSKQGLD 122

Query: 126 CAVCLSKFEDIEVLRLLPKCKHAFHIGCIDHWLEKHSSCPLCRCRISI-EDVAFFTYSNS 185
           C+VCLSKFE +E+LRLLPKC+HAFHIGCID WLE+H++CPLCR R+S+ ED +  T  NS
Sbjct: 123 CSVCLSKFESVEILRLLPKCRHAFHIGCIDQWLEQHATCPLCRDRVSMEEDSSVLTNGNS 182

Query: 186 MRLMRNNSQPELPQDSHMELFVQREEDHH--------NSSRFNTGSSFRKTGK-GNKEKE 245
            R +   +Q E+ +DS +EL+++REE+           SSRF+ G SFRK  K GNKEK 
Sbjct: 183 FRFL---NQSEIREDSSLELYIEREEEEERIHREELSGSSRFSIGESFRKILKLGNKEKT 242

Query: 246 VLIENEAGEPGSEERVLFHKHKHKILVSDFVFMNRWSNMSSSDLMFLNTEMLGAMSSNRF 305
           +L E+   +   +E+ L HK  H+I+VSD VF NRWSN+SSSDLMFLN+EM+ ++SS RF
Sbjct: 243 LLDEHVNDK---DEKKLMHKFNHRIVVSDVVFKNRWSNVSSSDLMFLNSEMVNSISSERF 302

Query: 306 SSLNSESIRRSATRETEGKRTSQIKEEMETKRSFERRISIAEKNSLVSISGIPSISDSNA 365
           SSL  + ++R    +  G    +IKEEME KR  E +++     S+ ++    S +  + 
Sbjct: 303 SSL--DHVKRGDEEDQIG--ILRIKEEMEAKRMLENKLT-----SMTTM--FSSENGDSG 362

Query: 366 STSRTTNLNE-RRSVSDTTAFSRF-----GHFNENAIRDSSMIE--ADAKDYRIRQVWLP 423
           S SR+  +   RRSVSD TA  R      G  + +A   +S ++   +  + R R++WLP
Sbjct: 363 SKSRSVMIEPGRRSVSDITAVPRLSISIHGDCSGSAAETASALQNGGNETEERRRRLWLP 422

BLAST of Lsi06G008290 vs. ExPASy Swiss-Prot
Match: Q9SL78 (Putative RING-H2 finger protein ATL12 OS=Arabidopsis thaliana OX=3702 GN=ATL12 PE=3 SV=1)

HSP 1 Score: 269.2 bits (687), Expect = 7.7e-71
Identity = 174/427 (40.75%), Postives = 251/427 (58.78%), Query Frame = 0

Query: 8   IMIVMTILLFPVNAQAPPPPK---AGSGIPQNFTIVIGILSIMFSMAFILVLYAKFCHQT 67
           I+    I L  V+AQ+PPPP           +  I+ G+ SI+F++ F+L++YAK  H  
Sbjct: 9   ILFFFIIFLDYVSAQSPPPPNLYATSDLFKPSLAIITGVFSIVFTLTFVLLVYAKCFH-- 68

Query: 68  SQLRGEPQA--------HLGLGLLRSDSGFSGVDQKVIDALPFFKFSSLKGSREGLECAV 127
           + LR E  +         L  GL    S FSG+D+K I++LPFF+FS+LKG ++GLEC+V
Sbjct: 69  NDLRSETDSDGERIRHDRLWQGLFNRSSRFSGLDKKAIESLPFFRFSALKGLKQGLECSV 128

Query: 128 CLSKFEDIEVLRLLPKCKHAFHIGCIDHWLEKHSSCPLCRCRISIEDVAFFTYSNSMRLM 187
           CLSKFED+E+LRLLPKC+HAFHIGCID WLE+H++CPLCR R++IED      ++S  L 
Sbjct: 129 CLSKFEDVEILRLLPKCRHAFHIGCIDQWLEQHATCPLCRNRVNIEDDLSVLGNSSTSLR 188

Query: 188 RNNSQPELPQDSHMELFVQREE-DHHNSSRFNTGSSFRKTGKGNKEKEVLIENEAGEPGS 247
             N      +DS +E++++REE  +  SSRF   SSFRK  K    K +L+E E G    
Sbjct: 189 ILNQSETREEDSRLEIYIEREEGTNDGSSRF---SSFRKILK----KSLLLERE-GNENI 248

Query: 248 EERVLFHKHKHKILVSDFVFMNRWSNMSSSDLMFLNTEMLGAMSSNRFSSLNSESIRRSA 307
           +E+ L HK  H+I+VSD VF NRWSN++SSDL FL +EML ++SS+RFSS+  + + R  
Sbjct: 249 DEKKLMHKFNHRIVVSDAVFKNRWSNITSSDLTFLTSEMLNSVSSDRFSSV--DRVHRGN 308

Query: 308 TRETEGKRTSQIKEEMETKRSFERRISIAEKNSLVSISGIPSISDSNASTSRTTNLNERR 367
            R+         KE+ME K     R+ I  K+S            S  + S  T ++  +
Sbjct: 309 LRD---------KEDMEMK-----RMLIKHKDS------------SRRTVSEITTVSREK 368

Query: 368 SVSDTTAFSRFGHFNENAIRDSSMIEADAKDYRIRQVWLPIAKRTVQWFANREQRSQVSK 423
           +V  +   S        A+  ++       + R R++WLPIA+RT QWF NRE+ + ++ 
Sbjct: 369 AVGGSYRGSTASTSQNYAVTATT-------EERRRRLWLPIARRTAQWFVNREKSNDLNT 390

BLAST of Lsi06G008290 vs. ExPASy Swiss-Prot
Match: Q5EAE9 (RING-H2 finger protein ATL43 OS=Arabidopsis thaliana OX=3702 GN=ATL43 PE=2 SV=2)

HSP 1 Score: 146.4 bits (368), Expect = 7.5e-34
Identity = 106/310 (34.19%), Postives = 154/310 (49.68%), Query Frame = 0

Query: 18  PVNAQAPPP-PKAGSGIPQNFTIVIGILSIMFSMAFILVLYAKFCHQT--SQLRGEPQAH 77
           P+   +PPP     S +     +VI +L+  FS+ F+L+LY K C +   S     PQ  
Sbjct: 38  PLPPPSPPPRHNFTSSLMPGIAVVIAVLTAFFSLTFLLLLYVKHCKRRNGSVYVNHPQR- 97

Query: 78  LGLGLLRSDSGF------------SGVDQKVIDALPFFKFSSLKGSREGLECAVCLSKFE 137
               + R   G+            SG+D+ VI++LP F+F +L G ++GLECAVCL++FE
Sbjct: 98  --FAITRYGGGYYNGGGVVGGRKNSGIDRSVIESLPVFRFGALSGHKDGLECAVCLARFE 157

Query: 138 DIEVLRLLPKCKHAFHIGCIDHWLEKHSSCPLCRCRISIEDVAFFTYSNSMRLMR----- 197
             EVLRLLPKCKHAFH+ C+D WL+ HS+CPLCR R+  ED+      NS   ++     
Sbjct: 158 PTEVLRLLPKCKHAFHVECVDTWLDAHSTCPLCRYRVDPEDILLIGDCNSWFELQFSKDE 217

Query: 198 NNSQPELPQDSHMELFVQREEDHHNS-----SRFN---TGSSFRKTGKGNKEK--EVLIE 257
           +NS    P      + V R    H+S     SR N   T SS++      +      L  
Sbjct: 218 SNSVNNNPPGLTRFIPVSRISGRHSSAGERASRLNEIRTSSSYKSNPMSFRRSLDSSLKV 277

Query: 258 NEAGEPGSE--------------------ERVLFH-KHKHKILVSDFVFMN-RWSNMSSS 276
           N+AGE  SE                     R  F  + +H+I++S     + RWS +  S
Sbjct: 278 NDAGEEKSESVAVNCLDRLQRKDGLLLIPNRESFEGRFEHRIIISGGNRDDQRWSEVRPS 337

BLAST of Lsi06G008290 vs. ExPASy Swiss-Prot
Match: Q8W571 (RING-H2 finger protein ATL32 OS=Arabidopsis thaliana OX=3702 GN=ATL32 PE=2 SV=3)

HSP 1 Score: 109.0 bits (271), Expect = 1.3e-22
Identity = 61/162 (37.65%), Postives = 91/162 (56.17%), Query Frame = 0

Query: 7   IIMIVMTILLFPVNAQ--APPPPKAGSGIPQNFTIVIGILSIMFSMAFILVLYAKFCHQT 66
           II+ V  I+    NAQ  +P PP   +G   + T V  +L  +F +  +L +Y + C   
Sbjct: 14  IILHVAIIIQSKANAQSFSPSPPDLQTGHTPSKTTVFAVLVTLFFLTGLLSVYIRHC--- 73

Query: 67  SQLRGEPQAHLGLGLLRSDSGFS---GVDQKVIDALPFFKFSSLKGSREG---LECAVCL 126
              R  P +       R++ G S   G+D  V+++ P F +SS+K S+ G   LECA+CL
Sbjct: 74  --ARSNPDSSTRYFRNRANDGSSRRGGLDNAVVESFPVFAYSSVKESKIGSKDLECAICL 133

Query: 127 SKFEDIEVLRLLPKCKHAFHIGCIDHWLEKHSSCPLCRCRIS 161
           ++ ED E +RLLP C H FHI CID WL  H++CP+CR  ++
Sbjct: 134 NELEDHETVRLLPICNHLFHIDCIDTWLYSHATCPVCRSNLT 170

BLAST of Lsi06G008290 vs. ExPASy Swiss-Prot
Match: Q9SRQ8 (RING-H2 finger protein ATL51 OS=Arabidopsis thaliana OX=3702 GN=ATL51 PE=2 SV=2)

HSP 1 Score: 103.6 bits (257), Expect = 5.6e-21
Identity = 62/220 (28.18%), Postives = 97/220 (44.09%), Query Frame = 0

Query: 24  PPPP---------KAGSGIPQNFTIVIGILSIMFSMAFILVLYAKFCHQTSQLRGEPQAH 83
           PPPP          + S        +IGIL+  F +     L +K+CH+         A 
Sbjct: 38  PPPPSFYLDDEDDSSSSDFSPLLIALIGILASAFILVSYYTLISKYCHRRRHNSSSTSA- 97

Query: 84  LGLGLLRSD-----------------------SGFSGVDQKVIDALPFFKFSSLKGSREG 143
             +  + SD                        G  G+D+ +I ++  +K+  + G  E 
Sbjct: 98  AAINRISSDYTWQGTNNNNNNGATNPNQTIGGGGGDGLDESLIKSITVYKYRKMDGFVES 157

Query: 144 LECAVCLSKFEDIEVLRLLPKCKHAFHIGCIDHWLEKHSSCPLCRCRISIEDVAFFTYSN 203
            +C+VCLS+F++ E LRLLPKC HAFH+ CID WL+ HS+CPLCR  I            
Sbjct: 158 SDCSVCLSEFQENESLRLLPKCNHAFHVPCIDTWLKSHSNCPLCRAFIVTSSAVEIVDLT 217

Query: 204 SMRLMRNNSQPELPQDSHMELFVQREEDHHNSSRFNTGSS 212
           + +++  N+      DS + + +  E     +   N GS+
Sbjct: 218 NQQIVTENNSISTGDDSVVVVNLDLENSRSRNETVNEGST 256

BLAST of Lsi06G008290 vs. ExPASy TrEMBL
Match: A0A6J1CUV0 (E3 ubiquitin-protein ligase ATL42-like OS=Momordica charantia OX=3673 GN=LOC111014428 PE=4 SV=1)

HSP 1 Score: 647.1 bits (1668), Expect = 5.0e-182
Identity = 338/422 (80.09%), Postives = 378/422 (89.57%), Query Frame = 0

Query: 1   MSKFSRIIMIVMTILLFPVNAQAPPPPKAGSGIPQNFTIVIGILSIMFSMAFILVLYAKF 60
           MSKFSRII+I+   LL   NAQ   PPKA SGIP+N T++IGI+SIM S+AFILVLYAKF
Sbjct: 1   MSKFSRIILIITATLLVHANAQV-SPPKADSGIPENLTMLIGIISIMLSLAFILVLYAKF 60

Query: 61  CHQTSQLRGEPQAHLGLGLLRSDSGFSGVDQKVIDALPFFKFSSLKGSREGLECAVCLSK 120
           CH+ S  RG P+AHLG+GLLRSD  FSGVDQKVID+LPFF+FSSLKGSREGLECAVCLSK
Sbjct: 61  CHRNS--RG-PRAHLGVGLLRSDLRFSGVDQKVIDSLPFFRFSSLKGSREGLECAVCLSK 120

Query: 121 FEDIEVLRLLPKCKHAFHIGCIDHWLEKHSSCPLCRCRISIEDVAFFTYSNSMRLMRNNS 180
           FEDIEVLRLLPKCKHAFHIGC+DHWLE HSSCP+CRCRIS+EDVAFFT SNSMRLMRNNS
Sbjct: 121 FEDIEVLRLLPKCKHAFHIGCVDHWLENHSSCPMCRCRISVEDVAFFTCSNSMRLMRNNS 180

Query: 181 QPELPQDSHMELFVQREEDHHNSSRFNTGSSFRKTGKGNKEKEVLIENEAGEPGSEERVL 240
           Q ELPQD +MELFVQREEDHHNSSRFN GSSF K GKG+KEK+      A E  SEE V+
Sbjct: 181 QSELPQDPNMELFVQREEDHHNSSRFNIGSSFGKIGKGSKEKD-----GARERQSEEHVV 240

Query: 241 FHKHKHKILVSDFVFMNRWSNMSSSDLMFLNTEMLGAMSSNRFSSLNSESIRRSATRETE 300
           FHKHKHKILVSDFVFMNRWSN+SSSDLMFLNTEMLGAMSSNRFSSL++++IRRS T ETE
Sbjct: 241 FHKHKHKILVSDFVFMNRWSNLSSSDLMFLNTEMLGAMSSNRFSSLDTDNIRRSETGETE 300

Query: 301 GKRTSQIKEEMETKRSFERRISIAEKNSLVSISGIPSISDSNASTSRTTNLNERRSVSDT 360
            KR S IKEEME KRSFE RISIAEKN+L+SI+G+PSISDSNASTSRT NLNERRSVSDT
Sbjct: 301 DKRISNIKEEMEMKRSFETRISIAEKNTLISITGMPSISDSNASTSRTINLNERRSVSDT 360

Query: 361 TAFSRFGHFNENAIRDSSMIEADAKDYRIRQVWLPIAKRTVQWFANREQRSQVSKYTQQP 420
           TAFSRFG+FNENAIR++S+IE++A+DYRIR++WLPIA+RTVQWFANRE R+Q+SKYTQQP
Sbjct: 361 TAFSRFGNFNENAIRETSVIESNAEDYRIRKIWLPIARRTVQWFANREHRAQLSKYTQQP 413

Query: 421 DV 423
           DV
Sbjct: 421 DV 413

BLAST of Lsi06G008290 vs. ExPASy TrEMBL
Match: A0A5D3DZA7 (E3 ubiquitin-protein ligase ATL42-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold403G00780 PE=4 SV=1)

HSP 1 Score: 603.2 bits (1554), Expect = 8.3e-169
Identity = 327/421 (77.67%), Postives = 351/421 (83.37%), Query Frame = 0

Query: 1   MSKFSRIIMIVMTILLFPVNAQAPPPPKAGSGIPQNFTIVIGILSIMFSMAFILVLYAKF 60
           M +F+RIIMI++ I LFPV AQAP PPKAGS IPQNFTIVIGILSIMFSM FILV+YAKF
Sbjct: 1   MGRFNRIIMIILIIFLFPVIAQAPSPPKAGSVIPQNFTIVIGILSIMFSMVFILVVYAKF 60

Query: 61  CHQTSQLRGEPQAHLGLGLLRSDSGFSGVDQKVIDALPFFKFSSLKGSREGLECAVCLSK 120
           CH TS  RGEPQA+L  GLLRSDS FSGVDQKVIDALPFF+FSSLKGSREGLECAVCLSK
Sbjct: 61  CHPTSHFRGEPQANLRHGLLRSDSRFSGVDQKVIDALPFFRFSSLKGSREGLECAVCLSK 120

Query: 121 FEDIEVLRLLPKCKHAFHIGCIDHWLEKHSSCPLCRCRISIEDVAFFTYSNSMRLMRNNS 180
           FEDIEVLRLLPKCKHAFHIGCIDHWLEKHSSCP+CRCRIS+EDVAFF  SNS+RL+RNNS
Sbjct: 121 FEDIEVLRLLPKCKHAFHIGCIDHWLEKHSSCPICRCRISVEDVAFFK-SNSIRLIRNNS 180

Query: 181 QPELPQDSHMELFVQREEDHHNSSRFNTGSSFRKTGKGNKEKEVLIENEAGEPGSEERVL 240
           QP+LPQDSHMELFV REE+HH SSR N G+SFRK GKG+KEKEVLIE+EA          
Sbjct: 181 QPDLPQDSHMELFVLREENHHYSSRSNDGTSFRKIGKGSKEKEVLIESEA---------- 240

Query: 241 FHKHKHKILVSDFVFMNRWSNMSSSDLMFLNTEMLGAMSSNRFSSLNSESIRRSATRETE 300
                                           EMLGAMSS+RFSSL SESIRRSATRE E
Sbjct: 241 --------------------------------EMLGAMSSSRFSSLESESIRRSATRERE 300

Query: 301 GKRTSQIKEEMETKRSFERRISIAEKNSLVSISGIPSISDSNASTSRTTNLNERRSVSDT 360
           GKRTSQIKE ME KRSFERRISIAEKNSLVSISGI S SDSNASTS+TTNLNE RSVSDT
Sbjct: 301 GKRTSQIKEAMEMKRSFERRISIAEKNSLVSISGISSTSDSNASTSKTTNLNE-RSVSDT 360

Query: 361 TAFSRFGHFNENAIRDSSMIEADAKDYRIRQVWLPIAKRTVQWFANREQRSQVSKYTQQP 420
           TAFSRFG+FNENAIRDSSMIEADAK YRIRQ+WLPIA+RTVQWFANRE+RS++SKYTQQP
Sbjct: 361 TAFSRFGYFNENAIRDSSMIEADAKSYRIRQLWLPIARRTVQWFANREERSRLSKYTQQP 377

Query: 421 D 422
           D
Sbjct: 421 D 377

BLAST of Lsi06G008290 vs. ExPASy TrEMBL
Match: A0A1S3CHH4 (E3 ubiquitin-protein ligase ATL42-like OS=Cucumis melo OX=3656 GN=LOC103500996 PE=4 SV=1)

HSP 1 Score: 578.2 bits (1489), Expect = 2.9e-161
Identity = 316/421 (75.06%), Postives = 338/421 (80.29%), Query Frame = 0

Query: 1   MSKFSRIIMIVMTILLFPVNAQAPPPPKAGSGIPQNFTIVIGILSIMFSMAFILVLYAKF 60
           M +F+RIIMI++ I LFPV AQAP PPKAGS IPQNFTIVIGILSIMFSM FILV+YAKF
Sbjct: 1   MGRFNRIIMIILIIFLFPVIAQAPSPPKAGSVIPQNFTIVIGILSIMFSMVFILVVYAKF 60

Query: 61  CHQTSQLRGEPQAHLGLGLLRSDSGFSGVDQKVIDALPFFKFSSLKGSREGLECAVCLSK 120
           CH TS  RGEPQA+L  GLLRSDS FSGVDQKVIDALPFF+FSSLKGSREGLECAVCLSK
Sbjct: 61  CHPTSHFRGEPQANLRHGLLRSDSRFSGVDQKVIDALPFFRFSSLKGSREGLECAVCLSK 120

Query: 121 FEDIEVLRLLPKCKHAFHIGCIDHWLEKHSSCPLCRCRISIEDVAFFTYSNSMRLMRNNS 180
           FEDIEVLRLLPKCKHAFHIGCIDHWLEKHSSCP+CRCRIS+EDVAFF  SNS+RL+RNNS
Sbjct: 121 FEDIEVLRLLPKCKHAFHIGCIDHWLEKHSSCPICRCRISVEDVAFFK-SNSIRLIRNNS 180

Query: 181 QPELPQDSHMELFVQREEDHHNSSRFNTGSSFRKTGKGNKEKEVLIENEAGEPGSEERVL 240
           QP+LPQDSHMELFV REE+HH SSR N G+SFRK GK                       
Sbjct: 181 QPDLPQDSHMELFVLREENHHYSSRSNDGTSFRKIGK----------------------- 240

Query: 241 FHKHKHKILVSDFVFMNRWSNMSSSDLMFLNTEMLGAMSSNRFSSLNSESIRRSATRETE 300
                                           EMLGAMSS+RFSSL SESIRRSATRE E
Sbjct: 241 --------------------------------EMLGAMSSSRFSSLESESIRRSATRERE 300

Query: 301 GKRTSQIKEEMETKRSFERRISIAEKNSLVSISGIPSISDSNASTSRTTNLNERRSVSDT 360
           GKRTSQIKE ME KRSFERRISIAEKNSLVSISGI S SDSNASTS+TTNLNE RSVSDT
Sbjct: 301 GKRTSQIKEAMEMKRSFERRISIAEKNSLVSISGISSTSDSNASTSKTTNLNE-RSVSDT 360

Query: 361 TAFSRFGHFNENAIRDSSMIEADAKDYRIRQVWLPIAKRTVQWFANREQRSQVSKYTQQP 420
           TAFSRFG+FNENAIRDSSMIEADAK YRIRQ+WLPIA+RTVQWFANRE+RS++SKYTQQP
Sbjct: 361 TAFSRFGYFNENAIRDSSMIEADAKSYRIRQLWLPIARRTVQWFANREERSRLSKYTQQP 364

Query: 421 D 422
           D
Sbjct: 421 D 364

BLAST of Lsi06G008290 vs. ExPASy TrEMBL
Match: A0A2N9GBZ7 (RING-type domain-containing protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS24653 PE=4 SV=1)

HSP 1 Score: 432.6 bits (1111), Expect = 1.9e-117
Identity = 236/417 (56.59%), Postives = 304/417 (72.90%), Query Frame = 0

Query: 7   IIMIVMTILLFPVNAQAPPPPKAGSGIPQNFTIVIGILSIMFSMAFILVLYAKFCHQTSQ 66
           +++ V+    F V AQ       GS  P + T+VIGILS+MFS+  +L++YAKFCH+   
Sbjct: 6   LLIFVLHFFFFHVKAQYTGD---GSASPGSLTVVIGILSLMFSLTILLLVYAKFCHRRGP 65

Query: 67  LRGEPQAHLGLGLLRSDSGFSGVDQKVIDALPFFKFSSLKGSREGLECAVCLSKFEDIEV 126
           +  +PQ      L+RS S FSG+D+ VI++LPFF+FSSLKGSR+GLECAVCLSKFED E+
Sbjct: 66  IHSDPQ---NFALIRSSSRFSGIDKTVIESLPFFRFSSLKGSRDGLECAVCLSKFEDTEI 125

Query: 127 LRLLPKCKHAFHIGCIDHWLEKHSSCPLCRCRISIEDVAFFTYSNSMRLMRNNSQPELPQ 186
           LRLLPKCKHAFHI CID+WLEKH+SCPLCR ++S ED + FTY+NSMRL+   +Q EL Q
Sbjct: 126 LRLLPKCKHAFHIECIDNWLEKHASCPLCRHKVSAEDPSIFTYTNSMRLI---NQSELRQ 185

Query: 187 DSHMELFVQREEDHHNSSRFNTGSSFRKTGKGNKEKEVLIENEAGEPGSEERVLFHKHKH 246
           DS++ELFVQREEDHH SSRF+ G+SFRKT KG+KE+  LI+ EA     E  ++ HKH H
Sbjct: 186 DSNIELFVQREEDHHGSSRFSIGNSFRKTEKGDKEENPLIQEEADNSFDENHIVLHKHNH 245

Query: 247 KILVSDFVFMNRWSNMSSSDLMFLNTEMLGAMSSNRFSSLNSESIRRSATRETEGKRTSQ 306
           KI+V DFVF NRWSN+SSSDLMFLN+EMLGA SS RF+S +  + + S  RE E     +
Sbjct: 246 KIIVPDFVFKNRWSNLSSSDLMFLNSEMLGATSSYRFASFDLNNEQSSTARENE--HIVK 305

Query: 307 IKEEMETKRSFERRISIAEKNSLVSISGIPSISDSNAS---TSRTTNLNERRSVSDTTAF 366
           IKEEM+ KR FE ++S   K S VS  GIPS SDS ++   TSR  N  ERRS+S+ TA 
Sbjct: 306 IKEEMDMKRLFENKVSTLNKTSAVSFPGIPSTSDSTSNLSQTSRYMNPTERRSMSEITAL 365

Query: 367 SRFGHFN-ENAIRDSSMIEADAKDYRIRQVWLPIAKRTVQWFANREQRSQVSKYTQQ 420
           SR+     +N I ++S++  + K  R+R++WLPIA+RTV W+ANRE+RSQ S+ TQQ
Sbjct: 366 SRYEDSGMKNRIGEASLLGNNVKAERMRRLWLPIARRTVHWYANRERRSQQSQNTQQ 411

BLAST of Lsi06G008290 vs. ExPASy TrEMBL
Match: A0A7N2KVY2 (RING-type domain-containing protein OS=Quercus lobata OX=97700 PE=4 SV=1)

HSP 1 Score: 430.3 bits (1105), Expect = 9.6e-117
Identity = 231/396 (58.33%), Postives = 293/396 (73.99%), Query Frame = 0

Query: 29  AGSGIPQNFTIVIGILSIMFSMAFILVLYAKFCHQTSQLRGEPQAHLGLGLLRSDSGFSG 88
           A   +  +  +VIGILSIMFS+ F+L++YAKFCH+   +  +PQ     G +RS S FSG
Sbjct: 24  ASQPVQPSLAVVIGILSIMFSLTFLLLVYAKFCHRRGSIHSDPQ---NFGFIRSSSRFSG 83

Query: 89  VDQKVIDALPFFKFSSLKGSREGLECAVCLSKFEDIEVLRLLPKCKHAFHIGCIDHWLEK 148
           +D+ VI++LPFF+FSSLKG++EGLEC+VCLSKFED E+LRLLPKCKHAFHI CID+WL K
Sbjct: 84  IDKTVIESLPFFRFSSLKGTKEGLECSVCLSKFEDTEMLRLLPKCKHAFHIDCIDNWLIK 143

Query: 149 HSSCPLCRCRISIEDVAFFTYSNSMRLMRNNSQPELPQDSHMELFVQREEDHHNSSRFNT 208
           HSSCPLCR ++S ED   FTY+NSMRLM   +Q EL QDS++ELFVQREEDHH SSRF+ 
Sbjct: 144 HSSCPLCRHKVSPEDPTIFTYTNSMRLM---NQSELRQDSNIELFVQREEDHHGSSRFSI 203

Query: 209 GSSFRKTGKGNKEKEVLIENEAGEPGSEERVLFHKHKHKILVSDFVFMNRWSNMSSSDLM 268
           GSSFRKT KGN E++ LI  E  +   E   + HKH HKI+VSDF+F NRWS++SSSDLM
Sbjct: 204 GSSFRKTEKGNTEEKPLIFQEEADNNDENNKVLHKHNHKIIVSDFIFNNRWSSVSSSDLM 263

Query: 269 FLNTEMLGAMSSNRFSSLNSESIRRSATRETEGKRTSQIKEEMETKRSFERRISIAEKNS 328
           FLN+E+L A SS RFSSL+  + + S  R  E     +IKEEME KRSFE ++S   K S
Sbjct: 264 FLNSELLSATSSYRFSSLDWNNEQSSTPRSLENGHIVKIKEEMELKRSFETKVSTLNKTS 323

Query: 329 LVSISGIPSISDSN---ASTSRTTNLNERRSVSDTTAFSRFGHFN--ENAIRDSSMIEAD 388
            VS  G+PS SDS    + TSR  N  ERRS+S+ TA SR+   +  +N IR+SS+IE +
Sbjct: 324 PVSFPGLPSTSDSTPNLSQTSRYMNPTERRSMSEITALSRYEDISGIKNRIRESSLIENN 383

Query: 389 AKDYRIRQVWLPIAKRTVQWFANREQRSQVSKYTQQ 420
            K  R+R++W PIAKRTV+W+ANRE+RSQ S+ TQ+
Sbjct: 384 VKAERMRRLWFPIAKRTVKWYANRERRSQQSQNTQR 413

BLAST of Lsi06G008290 vs. NCBI nr
Match: XP_031738779.1 (putative RING-H2 finger protein ATL12 [Cucumis sativus] >KAE8652892.1 hypothetical protein Csa_022677 [Cucumis sativus])

HSP 1 Score: 701.0 bits (1808), Expect = 6.0e-198
Identity = 365/422 (86.49%), Postives = 389/422 (92.18%), Query Frame = 0

Query: 1   MSKFSRIIMIVMTILLFPVNAQAPPPPKAGSGIPQNFTIVIGILSIMFSMAFILVLYAKF 60
           M KFSRIIMI++TI LFPV AQAP PPKAGSGIPQNFTIVIGILSIMFSM FILV+YAKF
Sbjct: 1   MGKFSRIIMIILTIFLFPVIAQAPSPPKAGSGIPQNFTIVIGILSIMFSMVFILVVYAKF 60

Query: 61  CHQTSQLRGEPQAHLGLGLLRSDSGFSGVDQKVIDALPFFKFSSLKGSREGLECAVCLSK 120
           CH TS  RGEPQAHLG GLLRSDS FSGVDQKVIDALPFF+FSSLKGSREGLECAVCLS 
Sbjct: 61  CHPTSHFRGEPQAHLGHGLLRSDSRFSGVDQKVIDALPFFRFSSLKGSREGLECAVCLSN 120

Query: 121 FEDIEVLRLLPKCKHAFHIGCIDHWLEKHSSCPLCRCRISIEDVAFFTYSNSMRLMRNNS 180
           FEDIEVLRLLPKCKHAFHIGCIDHWLEKHSSCP+CRCRI +ED+AFF  SNS+RL+RNNS
Sbjct: 121 FEDIEVLRLLPKCKHAFHIGCIDHWLEKHSSCPICRCRIGVEDIAFFK-SNSIRLIRNNS 180

Query: 181 QPELPQDSHMELFVQREEDHHNSSRFNTGSSFRKTGKGNKEKEVLIENEAGEPGSEERVL 240
           + ELPQDS +ELFVQREE+HHNSSR N G+SFR  GKGNKEKEVLIENEAGE  SEERVL
Sbjct: 181 RSELPQDSRIELFVQREENHHNSSRSNNGTSFRNIGKGNKEKEVLIENEAGECESEERVL 240

Query: 241 FHKHKHKILVSDFVFMNRWSNMSSSDLMFLNTEMLGAMSSNRFSSLNSESIRRSATRETE 300
           FHKHKHKILVSDFVFMNRWSN+SSSDL+FLNTEMLG MSSNRFSSL+SESI +SATRE E
Sbjct: 241 FHKHKHKILVSDFVFMNRWSNLSSSDLVFLNTEMLGVMSSNRFSSLDSESISQSATREKE 300

Query: 301 GKRTSQIKEEMETKRSFERRISIAEKNSLVSISGIPSI-SDSNASTSRTTNLNERRSVSD 360
           GK+TSQIKEEME KRSFERRISIAEKNSLVSISGI S  SDSNASTSRTT LNERRSVSD
Sbjct: 301 GKKTSQIKEEMEMKRSFERRISIAEKNSLVSISGISSTSSDSNASTSRTTILNERRSVSD 360

Query: 361 TTAFSRFGHFNENAIRDSSMIEADAKDYRIRQVWLPIAKRTVQWFANREQRSQVSKYTQQ 420
           TTAFSRFG+FNENA RDSSMIEADAK +RIRQ+WLPIA+RTVQWFANREQR ++SKYTQQ
Sbjct: 361 TTAFSRFGYFNENATRDSSMIEADAKSFRIRQLWLPIARRTVQWFANREQRFRLSKYTQQ 420

Query: 421 PD 422
           PD
Sbjct: 421 PD 421

BLAST of Lsi06G008290 vs. NCBI nr
Match: XP_031742649.1 (E3 ubiquitin-protein ligase ATL42-like, partial [Cucumis sativus])

HSP 1 Score: 682.2 bits (1759), Expect = 2.9e-192
Identity = 354/406 (87.19%), Postives = 376/406 (92.61%), Query Frame = 0

Query: 17  FPVNAQAPPPPKAGSGIPQNFTIVIGILSIMFSMAFILVLYAKFCHQTSQLRGEPQAHLG 76
           FPV AQAP PPKAGSGIPQNFTIVIGILSIMFSM FILV+YAKFCH TS  RGEPQAHLG
Sbjct: 5   FPVIAQAPSPPKAGSGIPQNFTIVIGILSIMFSMVFILVVYAKFCHPTSHFRGEPQAHLG 64

Query: 77  LGLLRSDSGFSGVDQKVIDALPFFKFSSLKGSREGLECAVCLSKFEDIEVLRLLPKCKHA 136
            GLLRSDS FSGVDQKVIDALPFF+FSSLKGSREGLECAVCLSKFEDIEVLRLLPKCKHA
Sbjct: 65  HGLLRSDSRFSGVDQKVIDALPFFRFSSLKGSREGLECAVCLSKFEDIEVLRLLPKCKHA 124

Query: 137 FHIGCIDHWLEKHSSCPLCRCRISIEDVAFFTYSNSMRLMRNNSQPELPQDSHMELFVQR 196
           FHIGCIDHWLEKHSSCP+CRCRI +ED+AFF  SNS+RL+RNNS+PELPQDS +ELFVQR
Sbjct: 125 FHIGCIDHWLEKHSSCPICRCRIGVEDIAFFK-SNSIRLIRNNSRPELPQDSRIELFVQR 184

Query: 197 EEDHHNSSRFNTGSSFRKTGKGNKEKEVLIENEAGEPGSEERVLFHKHKHKILVSDFVFM 256
           EE+HHNSSR N G+SFR  GKGNKEKEVLIENEAGE  SEERVLFHKHKHKILVSDFVFM
Sbjct: 185 EENHHNSSRSNNGTSFRNIGKGNKEKEVLIENEAGECESEERVLFHKHKHKILVSDFVFM 244

Query: 257 NRWSNMSSSDLMFLNTEMLGAMSSNRFSSLNSESIRRSATRETEGKRTSQIKEEMETKRS 316
           NRWSN+SSSDL+FLNTEMLG MSSNRFSSL+SESI +SATRE EGK+TSQIKEEME KRS
Sbjct: 245 NRWSNLSSSDLVFLNTEMLGVMSSNRFSSLDSESISQSATREKEGKKTSQIKEEMEMKRS 304

Query: 317 FERRISIAEKNSLVSISGIPSI-SDSNASTSRTTNLNERRSVSDTTAFSRFGHFNENAIR 376
           FERRISIAEKNSLVSISGI S  SD NASTSRTT LNERRSVSDTTAFSRFG+FNENA R
Sbjct: 305 FERRISIAEKNSLVSISGISSTSSDPNASTSRTTILNERRSVSDTTAFSRFGYFNENATR 364

Query: 377 DSSMIEADAKDYRIRQVWLPIAKRTVQWFANREQRSQVSKYTQQPD 422
           DSSMIEADAK +RIRQ+WLPIA+RTVQWFANREQR ++SKYTQQPD
Sbjct: 365 DSSMIEADAKSFRIRQLWLPIARRTVQWFANREQRFRLSKYTQQPD 409

BLAST of Lsi06G008290 vs. NCBI nr
Match: XP_022144842.1 (E3 ubiquitin-protein ligase ATL42-like [Momordica charantia])

HSP 1 Score: 647.1 bits (1668), Expect = 1.0e-181
Identity = 338/422 (80.09%), Postives = 378/422 (89.57%), Query Frame = 0

Query: 1   MSKFSRIIMIVMTILLFPVNAQAPPPPKAGSGIPQNFTIVIGILSIMFSMAFILVLYAKF 60
           MSKFSRII+I+   LL   NAQ   PPKA SGIP+N T++IGI+SIM S+AFILVLYAKF
Sbjct: 1   MSKFSRIILIITATLLVHANAQV-SPPKADSGIPENLTMLIGIISIMLSLAFILVLYAKF 60

Query: 61  CHQTSQLRGEPQAHLGLGLLRSDSGFSGVDQKVIDALPFFKFSSLKGSREGLECAVCLSK 120
           CH+ S  RG P+AHLG+GLLRSD  FSGVDQKVID+LPFF+FSSLKGSREGLECAVCLSK
Sbjct: 61  CHRNS--RG-PRAHLGVGLLRSDLRFSGVDQKVIDSLPFFRFSSLKGSREGLECAVCLSK 120

Query: 121 FEDIEVLRLLPKCKHAFHIGCIDHWLEKHSSCPLCRCRISIEDVAFFTYSNSMRLMRNNS 180
           FEDIEVLRLLPKCKHAFHIGC+DHWLE HSSCP+CRCRIS+EDVAFFT SNSMRLMRNNS
Sbjct: 121 FEDIEVLRLLPKCKHAFHIGCVDHWLENHSSCPMCRCRISVEDVAFFTCSNSMRLMRNNS 180

Query: 181 QPELPQDSHMELFVQREEDHHNSSRFNTGSSFRKTGKGNKEKEVLIENEAGEPGSEERVL 240
           Q ELPQD +MELFVQREEDHHNSSRFN GSSF K GKG+KEK+      A E  SEE V+
Sbjct: 181 QSELPQDPNMELFVQREEDHHNSSRFNIGSSFGKIGKGSKEKD-----GARERQSEEHVV 240

Query: 241 FHKHKHKILVSDFVFMNRWSNMSSSDLMFLNTEMLGAMSSNRFSSLNSESIRRSATRETE 300
           FHKHKHKILVSDFVFMNRWSN+SSSDLMFLNTEMLGAMSSNRFSSL++++IRRS T ETE
Sbjct: 241 FHKHKHKILVSDFVFMNRWSNLSSSDLMFLNTEMLGAMSSNRFSSLDTDNIRRSETGETE 300

Query: 301 GKRTSQIKEEMETKRSFERRISIAEKNSLVSISGIPSISDSNASTSRTTNLNERRSVSDT 360
            KR S IKEEME KRSFE RISIAEKN+L+SI+G+PSISDSNASTSRT NLNERRSVSDT
Sbjct: 301 DKRISNIKEEMEMKRSFETRISIAEKNTLISITGMPSISDSNASTSRTINLNERRSVSDT 360

Query: 361 TAFSRFGHFNENAIRDSSMIEADAKDYRIRQVWLPIAKRTVQWFANREQRSQVSKYTQQP 420
           TAFSRFG+FNENAIR++S+IE++A+DYRIR++WLPIA+RTVQWFANRE R+Q+SKYTQQP
Sbjct: 361 TAFSRFGNFNENAIRETSVIESNAEDYRIRKIWLPIARRTVQWFANREHRAQLSKYTQQP 413

Query: 421 DV 423
           DV
Sbjct: 421 DV 413

BLAST of Lsi06G008290 vs. NCBI nr
Match: KAE8647036.1 (hypothetical protein Csa_023000 [Cucumis sativus])

HSP 1 Score: 629.0 bits (1621), Expect = 2.9e-176
Identity = 326/376 (86.70%), Postives = 348/376 (92.55%), Query Frame = 0

Query: 47  MFSMAFILVLYAKFCHQTSQLRGEPQAHLGLGLLRSDSGFSGVDQKVIDALPFFKFSSLK 106
           MFSM FILV+YAKFCH TS  RGEPQAHLG GLLRSDS FSGVDQKVIDALPFF+FSSLK
Sbjct: 1   MFSMVFILVVYAKFCHPTSHFRGEPQAHLGHGLLRSDSRFSGVDQKVIDALPFFRFSSLK 60

Query: 107 GSREGLECAVCLSKFEDIEVLRLLPKCKHAFHIGCIDHWLEKHSSCPLCRCRISIEDVAF 166
           GSREGLECAVCLSKFEDIEVLRLLPKCKHAFHIGCIDHWLEKHSSCP+CRCRI +ED+AF
Sbjct: 61  GSREGLECAVCLSKFEDIEVLRLLPKCKHAFHIGCIDHWLEKHSSCPICRCRIGVEDIAF 120

Query: 167 FTYSNSMRLMRNNSQPELPQDSHMELFVQREEDHHNSSRFNTGSSFRKTGKGNKEKEVLI 226
           F  SNS+RL+RNNS+PELPQDS +ELFVQREE+HHNSSR N G+SFR  GKGNKEKEVLI
Sbjct: 121 FK-SNSIRLIRNNSRPELPQDSRIELFVQREENHHNSSRSNNGTSFRNIGKGNKEKEVLI 180

Query: 227 ENEAGEPGSEERVLFHKHKHKILVSDFVFMNRWSNMSSSDLMFLNTEMLGAMSSNRFSSL 286
           ENEAGE  SEERVLFHKHKHKILVSDFVFMNRWSN+SSSDL+FLNTEMLG MSSNRFSSL
Sbjct: 181 ENEAGECESEERVLFHKHKHKILVSDFVFMNRWSNLSSSDLVFLNTEMLGVMSSNRFSSL 240

Query: 287 NSESIRRSATRETEGKRTSQIKEEMETKRSFERRISIAEKNSLVSISGIPSI-SDSNAST 346
           +SESI +SATRE EGK+TSQIKEEME KRSFERRISIAEKNSLVSISGI S  SD NAST
Sbjct: 241 DSESISQSATREKEGKKTSQIKEEMEMKRSFERRISIAEKNSLVSISGISSTSSDPNAST 300

Query: 347 SRTTNLNERRSVSDTTAFSRFGHFNENAIRDSSMIEADAKDYRIRQVWLPIAKRTVQWFA 406
           SRTT LNERRSVSDTTAFSRFG+FNENA RDSSMIEADAK +RIRQ+WLPIA+RTVQWFA
Sbjct: 301 SRTTILNERRSVSDTTAFSRFGYFNENATRDSSMIEADAKSFRIRQLWLPIARRTVQWFA 360

Query: 407 NREQRSQVSKYTQQPD 422
           NREQR ++SKYTQQPD
Sbjct: 361 NREQRFRLSKYTQQPD 375

BLAST of Lsi06G008290 vs. NCBI nr
Match: KAA0046520.1 (E3 ubiquitin-protein ligase ATL42-like [Cucumis melo var. makuwa] >TYK28739.1 E3 ubiquitin-protein ligase ATL42-like [Cucumis melo var. makuwa])

HSP 1 Score: 603.2 bits (1554), Expect = 1.7e-168
Identity = 327/421 (77.67%), Postives = 351/421 (83.37%), Query Frame = 0

Query: 1   MSKFSRIIMIVMTILLFPVNAQAPPPPKAGSGIPQNFTIVIGILSIMFSMAFILVLYAKF 60
           M +F+RIIMI++ I LFPV AQAP PPKAGS IPQNFTIVIGILSIMFSM FILV+YAKF
Sbjct: 1   MGRFNRIIMIILIIFLFPVIAQAPSPPKAGSVIPQNFTIVIGILSIMFSMVFILVVYAKF 60

Query: 61  CHQTSQLRGEPQAHLGLGLLRSDSGFSGVDQKVIDALPFFKFSSLKGSREGLECAVCLSK 120
           CH TS  RGEPQA+L  GLLRSDS FSGVDQKVIDALPFF+FSSLKGSREGLECAVCLSK
Sbjct: 61  CHPTSHFRGEPQANLRHGLLRSDSRFSGVDQKVIDALPFFRFSSLKGSREGLECAVCLSK 120

Query: 121 FEDIEVLRLLPKCKHAFHIGCIDHWLEKHSSCPLCRCRISIEDVAFFTYSNSMRLMRNNS 180
           FEDIEVLRLLPKCKHAFHIGCIDHWLEKHSSCP+CRCRIS+EDVAFF  SNS+RL+RNNS
Sbjct: 121 FEDIEVLRLLPKCKHAFHIGCIDHWLEKHSSCPICRCRISVEDVAFFK-SNSIRLIRNNS 180

Query: 181 QPELPQDSHMELFVQREEDHHNSSRFNTGSSFRKTGKGNKEKEVLIENEAGEPGSEERVL 240
           QP+LPQDSHMELFV REE+HH SSR N G+SFRK GKG+KEKEVLIE+EA          
Sbjct: 181 QPDLPQDSHMELFVLREENHHYSSRSNDGTSFRKIGKGSKEKEVLIESEA---------- 240

Query: 241 FHKHKHKILVSDFVFMNRWSNMSSSDLMFLNTEMLGAMSSNRFSSLNSESIRRSATRETE 300
                                           EMLGAMSS+RFSSL SESIRRSATRE E
Sbjct: 241 --------------------------------EMLGAMSSSRFSSLESESIRRSATRERE 300

Query: 301 GKRTSQIKEEMETKRSFERRISIAEKNSLVSISGIPSISDSNASTSRTTNLNERRSVSDT 360
           GKRTSQIKE ME KRSFERRISIAEKNSLVSISGI S SDSNASTS+TTNLNE RSVSDT
Sbjct: 301 GKRTSQIKEAMEMKRSFERRISIAEKNSLVSISGISSTSDSNASTSKTTNLNE-RSVSDT 360

Query: 361 TAFSRFGHFNENAIRDSSMIEADAKDYRIRQVWLPIAKRTVQWFANREQRSQVSKYTQQP 420
           TAFSRFG+FNENAIRDSSMIEADAK YRIRQ+WLPIA+RTVQWFANRE+RS++SKYTQQP
Sbjct: 361 TAFSRFGYFNENAIRDSSMIEADAKSYRIRQLWLPIARRTVQWFANREERSRLSKYTQQP 377

Query: 421 D 422
           D
Sbjct: 421 D 377

BLAST of Lsi06G008290 vs. TAIR 10
Match: AT4G28890.1 (RING/U-box superfamily protein )

HSP 1 Score: 303.5 bits (776), Expect = 2.6e-82
Identity = 192/447 (42.95%), Postives = 282/447 (63.09%), Query Frame = 0

Query: 6   RIIMIVMTILLFPVNAQAPPPPKAG---SGIPQNFTIVIGILSIMFSMAFILVLYAKFCH 65
           +I    + +L    +AQ PPP + G   +    +  +V G+L+IMF++ F+L++YAK CH
Sbjct: 3   QIFFFFLPLLHSYASAQTPPPFRNGDLVANFEPSLAVVTGVLAIMFALTFVLLVYAKCCH 62

Query: 66  ------QTSQLRGEPQAHLGLGLLRSDSG---FSGVDQKVIDALPFFKFSSLKGSREGLE 125
                    + R + +   G+   RS +    FSG+D+  I++LP F+FS+LKGS++GL+
Sbjct: 63  IDLRSGSGDRRRHDRRLRQGIFFNRSTASSDRFSGLDKTAIESLPLFRFSALKGSKQGLD 122

Query: 126 CAVCLSKFEDIEVLRLLPKCKHAFHIGCIDHWLEKHSSCPLCRCRISI-EDVAFFTYSNS 185
           C+VCLSKFE +E+LRLLPKC+HAFHIGCID WLE+H++CPLCR R+S+ ED +  T  NS
Sbjct: 123 CSVCLSKFESVEILRLLPKCRHAFHIGCIDQWLEQHATCPLCRDRVSMEEDSSVLTNGNS 182

Query: 186 MRLMRNNSQPELPQDSHMELFVQREEDHH--------NSSRFNTGSSFRKTGK-GNKEKE 245
            R +   +Q E+ +DS +EL+++REE+           SSRF+ G SFRK  K GNKEK 
Sbjct: 183 FRFL---NQSEIREDSSLELYIEREEEEERIHREELSGSSRFSIGESFRKILKLGNKEKT 242

Query: 246 VLIENEAGEPGSEERVLFHKHKHKILVSDFVFMNRWSNMSSSDLMFLNTEMLGAMSSNRF 305
           +L E+   +   +E+ L HK  H+I+VSD VF NRWSN+SSSDLMFLN+EM+ ++SS RF
Sbjct: 243 LLDEHVNDK---DEKKLMHKFNHRIVVSDVVFKNRWSNVSSSDLMFLNSEMVNSISSERF 302

Query: 306 SSLNSESIRRSATRETEGKRTSQIKEEMETKRSFERRISIAEKNSLVSISGIPSISDSNA 365
           SSL  + ++R    +  G    +IKEEME KR  E +++     S+ ++    S +  + 
Sbjct: 303 SSL--DHVKRGDEEDQIG--ILRIKEEMEAKRMLENKLT-----SMTTM--FSSENGDSG 362

Query: 366 STSRTTNLNE-RRSVSDTTAFSRF-----GHFNENAIRDSSMIE--ADAKDYRIRQVWLP 423
           S SR+  +   RRSVSD TA  R      G  + +A   +S ++   +  + R R++WLP
Sbjct: 363 SKSRSVMIEPGRRSVSDITAVPRLSISIHGDCSGSAAETASALQNGGNETEERRRRLWLP 422

BLAST of Lsi06G008290 vs. TAIR 10
Match: AT2G20030.1 (RING/U-box superfamily protein )

HSP 1 Score: 269.2 bits (687), Expect = 5.5e-72
Identity = 174/427 (40.75%), Postives = 251/427 (58.78%), Query Frame = 0

Query: 8   IMIVMTILLFPVNAQAPPPPK---AGSGIPQNFTIVIGILSIMFSMAFILVLYAKFCHQT 67
           I+    I L  V+AQ+PPPP           +  I+ G+ SI+F++ F+L++YAK  H  
Sbjct: 9   ILFFFIIFLDYVSAQSPPPPNLYATSDLFKPSLAIITGVFSIVFTLTFVLLVYAKCFH-- 68

Query: 68  SQLRGEPQA--------HLGLGLLRSDSGFSGVDQKVIDALPFFKFSSLKGSREGLECAV 127
           + LR E  +         L  GL    S FSG+D+K I++LPFF+FS+LKG ++GLEC+V
Sbjct: 69  NDLRSETDSDGERIRHDRLWQGLFNRSSRFSGLDKKAIESLPFFRFSALKGLKQGLECSV 128

Query: 128 CLSKFEDIEVLRLLPKCKHAFHIGCIDHWLEKHSSCPLCRCRISIEDVAFFTYSNSMRLM 187
           CLSKFED+E+LRLLPKC+HAFHIGCID WLE+H++CPLCR R++IED      ++S  L 
Sbjct: 129 CLSKFEDVEILRLLPKCRHAFHIGCIDQWLEQHATCPLCRNRVNIEDDLSVLGNSSTSLR 188

Query: 188 RNNSQPELPQDSHMELFVQREE-DHHNSSRFNTGSSFRKTGKGNKEKEVLIENEAGEPGS 247
             N      +DS +E++++REE  +  SSRF   SSFRK  K    K +L+E E G    
Sbjct: 189 ILNQSETREEDSRLEIYIEREEGTNDGSSRF---SSFRKILK----KSLLLERE-GNENI 248

Query: 248 EERVLFHKHKHKILVSDFVFMNRWSNMSSSDLMFLNTEMLGAMSSNRFSSLNSESIRRSA 307
           +E+ L HK  H+I+VSD VF NRWSN++SSDL FL +EML ++SS+RFSS+  + + R  
Sbjct: 249 DEKKLMHKFNHRIVVSDAVFKNRWSNITSSDLTFLTSEMLNSVSSDRFSSV--DRVHRGN 308

Query: 308 TRETEGKRTSQIKEEMETKRSFERRISIAEKNSLVSISGIPSISDSNASTSRTTNLNERR 367
            R+         KE+ME K     R+ I  K+S            S  + S  T ++  +
Sbjct: 309 LRD---------KEDMEMK-----RMLIKHKDS------------SRRTVSEITTVSREK 368

Query: 368 SVSDTTAFSRFGHFNENAIRDSSMIEADAKDYRIRQVWLPIAKRTVQWFANREQRSQVSK 423
           +V  +   S        A+  ++       + R R++WLPIA+RT QWF NRE+ + ++ 
Sbjct: 369 AVGGSYRGSTASTSQNYAVTATT-------EERRRRLWLPIARRTAQWFVNREKSNDLNT 390

BLAST of Lsi06G008290 vs. TAIR 10
Match: AT5G05810.1 (RING/U-box superfamily protein )

HSP 1 Score: 141.7 bits (356), Expect = 1.3e-33
Identity = 101/288 (35.07%), Postives = 146/288 (50.69%), Query Frame = 0

Query: 39  IVIGILSIMFSMAFILVLYAKFCHQT--SQLRGEPQAHLGLGLLRSDSGF---------- 98
           +VI +L+  FS+ F+L+LY K C +   S     PQ      + R   G+          
Sbjct: 6   VVIAVLTAFFSLTFLLLLYVKHCKRRNGSVYVNHPQR---FAITRYGGGYYNGGGVVGGR 65

Query: 99  --SGVDQKVIDALPFFKFSSLKGSREGLECAVCLSKFEDIEVLRLLPKCKHAFHIGCIDH 158
             SG+D+ VI++LP F+F +L G ++GLECAVCL++FE  EVLRLLPKCKHAFH+ C+D 
Sbjct: 66  KNSGIDRSVIESLPVFRFGALSGHKDGLECAVCLARFEPTEVLRLLPKCKHAFHVECVDT 125

Query: 159 WLEKHSSCPLCRCRISIEDVAFFTYSNSMRLMR-----NNSQPELPQDSHMELFVQREED 218
           WL+ HS+CPLCR R+  ED+      NS   ++     +NS    P      + V R   
Sbjct: 126 WLDAHSTCPLCRYRVDPEDILLIGDCNSWFELQFSKDESNSVNNNPPGLTRFIPVSRISG 185

Query: 219 HHNS-----SRFN---TGSSFRKTGKGNKEK--EVLIENEAGEPGSE------------- 276
            H+S     SR N   T SS++      +      L  N+AGE  SE             
Sbjct: 186 RHSSAGERASRLNEIRTSSSYKSNPMSFRRSLDSSLKVNDAGEEKSESVAVNCLDRLQRK 245

BLAST of Lsi06G008290 vs. TAIR 10
Match: AT4G40070.1 (RING/U-box superfamily protein )

HSP 1 Score: 109.0 bits (271), Expect = 9.4e-24
Identity = 61/162 (37.65%), Postives = 91/162 (56.17%), Query Frame = 0

Query: 7   IIMIVMTILLFPVNAQ--APPPPKAGSGIPQNFTIVIGILSIMFSMAFILVLYAKFCHQT 66
           II+ V  I+    NAQ  +P PP   +G   + T V  +L  +F +  +L +Y + C   
Sbjct: 14  IILHVAIIIQSKANAQSFSPSPPDLQTGHTPSKTTVFAVLVTLFFLTGLLSVYIRHC--- 73

Query: 67  SQLRGEPQAHLGLGLLRSDSGFS---GVDQKVIDALPFFKFSSLKGSREG---LECAVCL 126
              R  P +       R++ G S   G+D  V+++ P F +SS+K S+ G   LECA+CL
Sbjct: 74  --ARSNPDSSTRYFRNRANDGSSRRGGLDNAVVESFPVFAYSSVKESKIGSKDLECAICL 133

Query: 127 SKFEDIEVLRLLPKCKHAFHIGCIDHWLEKHSSCPLCRCRIS 161
           ++ ED E +RLLP C H FHI CID WL  H++CP+CR  ++
Sbjct: 134 NELEDHETVRLLPICNHLFHIDCIDTWLYSHATCPVCRSNLT 170

BLAST of Lsi06G008290 vs. TAIR 10
Match: AT3G03550.1 (RING/U-box superfamily protein )

HSP 1 Score: 103.6 bits (257), Expect = 4.0e-22
Identity = 62/220 (28.18%), Postives = 97/220 (44.09%), Query Frame = 0

Query: 24  PPPP---------KAGSGIPQNFTIVIGILSIMFSMAFILVLYAKFCHQTSQLRGEPQAH 83
           PPPP          + S        +IGIL+  F +     L +K+CH+         A 
Sbjct: 38  PPPPSFYLDDEDDSSSSDFSPLLIALIGILASAFILVSYYTLISKYCHRRRHNSSSTSA- 97

Query: 84  LGLGLLRSD-----------------------SGFSGVDQKVIDALPFFKFSSLKGSREG 143
             +  + SD                        G  G+D+ +I ++  +K+  + G  E 
Sbjct: 98  AAINRISSDYTWQGTNNNNNNGATNPNQTIGGGGGDGLDESLIKSITVYKYRKMDGFVES 157

Query: 144 LECAVCLSKFEDIEVLRLLPKCKHAFHIGCIDHWLEKHSSCPLCRCRISIEDVAFFTYSN 203
            +C+VCLS+F++ E LRLLPKC HAFH+ CID WL+ HS+CPLCR  I            
Sbjct: 158 SDCSVCLSEFQENESLRLLPKCNHAFHVPCIDTWLKSHSNCPLCRAFIVTSSAVEIVDLT 217

Query: 204 SMRLMRNNSQPELPQDSHMELFVQREEDHHNSSRFNTGSS 212
           + +++  N+      DS + + +  E     +   N GS+
Sbjct: 218 NQQIVTENNSISTGDDSVVVVNLDLENSRSRNETVNEGST 256

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q5XF853.7e-8142.95E3 ubiquitin-protein ligase ATL42 OS=Arabidopsis thaliana OX=3702 GN=ATL42 PE=1 ... [more]
Q9SL787.7e-7140.75Putative RING-H2 finger protein ATL12 OS=Arabidopsis thaliana OX=3702 GN=ATL12 P... [more]
Q5EAE97.5e-3434.19RING-H2 finger protein ATL43 OS=Arabidopsis thaliana OX=3702 GN=ATL43 PE=2 SV=2[more]
Q8W5711.3e-2237.65RING-H2 finger protein ATL32 OS=Arabidopsis thaliana OX=3702 GN=ATL32 PE=2 SV=3[more]
Q9SRQ85.6e-2128.18RING-H2 finger protein ATL51 OS=Arabidopsis thaliana OX=3702 GN=ATL51 PE=2 SV=2[more]
Match NameE-valueIdentityDescription
A0A6J1CUV05.0e-18280.09E3 ubiquitin-protein ligase ATL42-like OS=Momordica charantia OX=3673 GN=LOC1110... [more]
A0A5D3DZA78.3e-16977.67E3 ubiquitin-protein ligase ATL42-like OS=Cucumis melo var. makuwa OX=1194695 GN... [more]
A0A1S3CHH42.9e-16175.06E3 ubiquitin-protein ligase ATL42-like OS=Cucumis melo OX=3656 GN=LOC103500996 P... [more]
A0A2N9GBZ71.9e-11756.59RING-type domain-containing protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS2465... [more]
A0A7N2KVY29.6e-11758.33RING-type domain-containing protein OS=Quercus lobata OX=97700 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
XP_031738779.16.0e-19886.49putative RING-H2 finger protein ATL12 [Cucumis sativus] >KAE8652892.1 hypothetic... [more]
XP_031742649.12.9e-19287.19E3 ubiquitin-protein ligase ATL42-like, partial [Cucumis sativus][more]
XP_022144842.11.0e-18180.09E3 ubiquitin-protein ligase ATL42-like [Momordica charantia][more]
KAE8647036.12.9e-17686.70hypothetical protein Csa_023000 [Cucumis sativus][more]
KAA0046520.11.7e-16877.67E3 ubiquitin-protein ligase ATL42-like [Cucumis melo var. makuwa] >TYK28739.1 E3... [more]
Match NameE-valueIdentityDescription
AT4G28890.12.6e-8242.95RING/U-box superfamily protein [more]
AT2G20030.15.5e-7240.75RING/U-box superfamily protein [more]
AT5G05810.11.3e-3335.07RING/U-box superfamily protein [more]
AT4G40070.19.4e-2437.65RING/U-box superfamily protein [more]
AT3G03550.14.0e-2228.18RING/U-box superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 307..327
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 288..313
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 293..313
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 200..219
NoneNo IPR availablePANTHERPTHR46539E3 UBIQUITIN-PROTEIN LIGASE ATL42coord: 29..415
NoneNo IPR availablePANTHERPTHR46539:SF1E3 UBIQUITIN-PROTEIN LIGASE ATL42coord: 29..415
NoneNo IPR availableCDDcd16454RING-H2_PA-TM-RINGcoord: 113..156
e-value: 6.15019E-21
score: 83.5053
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 93..161
IPR001841Zinc finger, RING-typeSMARTSM00184ring_2coord: 114..155
e-value: 7.3E-7
score: 38.8
IPR001841Zinc finger, RING-typePFAMPF13639zf-RING_2coord: 113..156
e-value: 1.9E-12
score: 47.4
IPR001841Zinc finger, RING-typePROSITEPS50089ZF_RING_2coord: 114..156
score: 12.474524
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 88..164
e-value: 1.3E-21
score: 77.6

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi06G008290.1Lsi06G008290.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane