Lsi06G008270 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi06G008270
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionProtein-serine/threonine phosphatase
Locationchr06: 17574255 .. 17578005 (+)
RNA-Seq ExpressionLsi06G008270
SyntenyLsi06G008270
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCCTCCATCTCCAGCTCACAGGAAGTCATGGGAAGAAGAAGTAAGCTCATGTCTTGCTTCTAATATATCTTGATTTTCAAAGAAAATCAGTACAGATTTGTTTCATTTGTTTGTTATTTACTTATTTACCTCGAATGAACCTTTGAGGTGCTGAGTTTTTGGCTTCATACTCCACAGGCACATATTGAACGAGCATTGCACGACCATTTCCTCTTCAGAAAACTAACTGATTCTCAGTGCCAAGTTTTATTAGATTGCATGCAAAGAGTTGAGGTCACACCCGGGCAAATTGTAGTCGAACAAGTAGTTTTCTTTCTTTTTCTTGTTTTTTTTTTTTTCCTTCCCTTGTCATGAAAGCCAACTGCCATCCTTAAATCAAATACATATGTGCAAATGAGAATTTAAGGACAACTATTTTTATATGGTGGATTTTACATGGAAGAATGAAGATGCCAATTGTTTGTTATGTGCACGGCCTGTGTTGGTTTGTAGGGTGGCGAAGGTGACTGTTTTTATGTGGTTGGCAGTGGAGAATTTGAGGTCTTGGCAACCCAGGTTCATATCATTTTTCTTTCTTTTGATCCCTTATATTTCATATTCAAGGCATTTTCTTGAGACTTTATGTTGACTCAATGATATCATCACAATTACAGGAAGAAAGCCACGGAGAGGTTCCTCGGATTCTGCAACACTATACAGCTGAGAAGCTCTCTTCCTTCGGGGAACTGGCTTTGATGTGGGTTTTCTCTTGTTAATGCACGTACTTTATCATCTATGGGTAGTACTTATTTTGTTTAATGAGTTTGTTATGCGTATAATGTATATACAGGTATAACAAACCACTTCAAGCCTCGGTACGTGCTGTGACAAGTGGGACACTTTGGGCTTTGAAAAGAGAGGACTTTCGTGGAATTCTTATATCAGAATTTTCTAACTTGTCATCTTTGAAATTGCTTCGATCTGTGGACCTTTTGTCGAAGTTAACAATCTTGCAATTGAGTCAAATTGCAGACTCCCTTTCTGAAGTTCACTTCTCAGATGGGGAGCTGATTGTTGATGGGGTAACCTCATTTTTTAACAGCACGTCACTGACAATTTTTATTTTCTTTACAACTCTTCAAGTATTTTTATTTATTTATTTATTTTTGCAGACAGAAGACTTATGTTCACTGCACATTATTCAAAAGGGACAAGTGAGGATTACTTTTGATGCGGAGTTAATGAGCAGTTCAAATGTTTACAGCTTCAATTATGCCAGTCAAAGAGAGGACAATGCTATGCAGAGTGATAACGAGATCTCAGCTATTAGGAAAGAGGGAAGTTATTTTGGCGAATGGGCACTTCTTGGTGAGCGTATCGGCTTCTTACGTGCAGTTGCTGTGGGAGATGTTGTGTGTGCTATTTTAACAAAGGAAAAGTTTGAATCAGTTGTTGGCCCTTTACCAAATCTCTCTCAAGATGATCAAAAGTATATTGCATTTGCTTTTGAGTTGTATGGGCACACTTTTATCATATTTTTTCTTCAGTCATATTCTAGCCTGTACACATTGTTTGACTTCATGTAGGGTGAAAGAACACTCTTCAAACTCTCTCCATCAATCTGCCAAAATTATGGATATTTCAGCTCTTTCAAAAGTTGAGCTCTCTGATCTGGTGAGAATAAATTTTGCTGTTTGTGGTTTTGTTACTGTAACTATGTTTTCCCCCTTTTTCTCCGGAAACTGACACCTTATGTTATCTTCTTTGTTTGTTTTTGTTTATAGGAGTGGAAAATGTGTTTATATTCCACGGAATACAGTGAAATTGGGCTAGTTCGGTTAAGGAACACAGGTTATCTTGTAGTATCTCGTCAATCTTCTTTATAATGGCTATGAATAATGTTCTTTGATGTCTATTTGTCAAGCTGATGGAACTGCCTTGGTGAATTTGAAGCAGAAACTATGCTTAGTTTAAAAAGGTTTACAAGAGAGAAGGTCAAAAGGCTGGGACTTGAAGCACAAGTTTTGAAGGAAAAAAATCTTATGAAGAGCATCAGTTCTTCAGCTTGCGTGCCGCAACTTCTCTGCACATGTTTTGATCAATCACATGCTGGCATATTGCTAAAGACATGCCTTGCTTGTCCTTTGTCTTCGATACTTCATGTCCCACTCGATGAATCTTCTGCACGTTTCTTTGCAGCCTCTCTTGTTATGGCACTGGAAGATTTGCACAAGGTTTGATTCTACGGACTTGCTGTTGGTCCTCTTTTCTTCTTCAATGCCGAATCAATTTTCGCCTTGGATTTTTCTGCAATTTTCTCTTTCTATATCCCCTCTTCATTTGAATTTTCAGCTCGGTGTTCTCCACAGAGGAATCTCCCCTGATGTTCTAATGTTGGATCAAAATGGACGCATACAGGTCTATACCTGCTGTGATTTTTTTTCCTACATGAACATATTTTTCAAGGTCTCTTCTTAAAAAATAGCAGTCCGTAGAACCTCACTTATATTTCTTGTATTCCTCTTATTGCAGTTAGTAGACTTCAGATTTGGGAAAAGAACTTTGGGCGAGAGAACATATACTATTTGCGGGATGGCCGACTTTTTAGCACCCGAGATAGTTCAAGGCAATGGTCATGGTTTTGCTGCTGACAGGTAAAAATGCAATTTTGTTTGCACTTTGATAAGGTCATCTTGAAAATAACTCCCAATGCTTACATAATCATAAATGAGAACATATTCAAGCCATGGTGTGTTATATTGGACTTGGCCACTTTCACTTCACTTGAACTTACAACTCACTAACCACGTTGAAAACGCCCAGGTGGGCAGTGGGAGTCTTAATCTATTTCATGCTGAAAGGTGAAATGCCATTTGGATCGTGGCGACGAAGCGAGCTCGATACTTTTTCAAAGATTGCAAAAGGACAGTTAAGTCTTCCCCAAATTTTCAGTCCTGAAGCAATGGATCTCATCGTCAAGGTAATGCTAGTGATCAACAATCTCCTCTACCCTTTCCCATTCCCATGTACTTTGCTAAGCCAATCATATATAGTAATTTTCACCTTTTGTAATAAAAAATATGATCAGTTACTTGAAGTTGATGAGAAGAAAAGACTTGGAAACGAGAACCAAAACTCTGTTAGAAGTCATCCATGGTTTGATGGCGTTGATTGGAAGGGGATCCATGACGGTACCTTTCCCGTTCCTGAGACAATAACTTCTCGTGTAGCTCAATATTTGGAGAGCTACCCTGAAGATTGCAGTGTTTCTTTAATTAAACCACCTCAAGATCTTGAAGAACAGGATGCCCCCGATTGGATCAACGACTGGTAGAAGTACAACACCGACAAAACTCTCCATCAAAATATGTATGCAGGCTTTTTTCAGGTGGAGTCTGTCTTCTGCTGATCGGTTCCCGTTGTTCTCAGTCAACACGAATCGATAATTTGAGAAATCCTTCGATATAATGCAAGTAAGTTGCATCGTATCAAGTTCATGTGAAGATTAGGTTGGTGAATAGATGTTATTTTTCCCATTTTTGTCTGTTGAAAATTGATTTCTGTGAATACAGAGTAGGTATATATTATATATGTAAATGAAGTAGAGTTCATTTTTTTGCTCTACAGTAAAATTGTTACTTGTCTTGTGGAACCTTCTGTTACTGGCTGCTGATAATAAATCCATCTTCTCCAGCCAGTGGTTTTATTGTTGGTTTTCTAGAGTTCATTCAAACTTAATATATTGCCTCTCTCTTCCTTTTGATTTTGTG

mRNA sequence

ATGCCTCCATCTCCAGCTCACAGGAAGTCATGGGAAGAAGAAGCACATATTGAACGAGCATTGCACGACCATTTCCTCTTCAGAAAACTAACTGATTCTCAGTGCCAAGTTTTATTAGATTGCATGCAAAGAGTTGAGGTCACACCCGGGCAAATTGTAGTCGAACAAGGTGGCGAAGGTGACTGTTTTTATGTGGTTGGCAGTGGAGAATTTGAGGTCTTGGCAACCCAGGAAGAAAGCCACGGAGAGGTTCCTCGGATTCTGCAACACTATACAGCTGAGAAGCTCTCTTCCTTCGGGGAACTGGCTTTGATGTATAACAAACCACTTCAAGCCTCGGTACGTGCTGTGACAAGTGGGACACTTTGGGCTTTGAAAAGAGAGGACTTTCGTGGAATTCTTATATCAGAATTTTCTAACTTGTCATCTTTGAAATTGCTTCGATCTGTGGACCTTTTGTCGAAGTTAACAATCTTGCAATTGAGTCAAATTGCAGACTCCCTTTCTGAAGTTCACTTCTCAGATGGGGAGCTGATTGTTGATGGGACAGAAGACTTATGTTCACTGCACATTATTCAAAAGGGACAAGTGAGGATTACTTTTGATGCGGAGTTAATGAGCAGTTCAAATGTTTACAGCTTCAATTATGCCAGTCAAAGAGAGGACAATGCTATGCAGAGTGATAACGAGATCTCAGCTATTAGGAAAGAGGGAAGTTATTTTGGCGAATGGGCACTTCTTGGTGAGCGTATCGGCTTCTTACGTGCAGTTGCTGTGGGAGATGTTGTGTGTGCTATTTTAACAAAGGAAAAGTTTGAATCAGTTGTTGGCCCTTTACCAAATCTCTCTCAAGATGATCAAAAGGTGAAAGAACACTCTTCAAACTCTCTCCATCAATCTGCCAAAATTATGGATATTTCAGCTCTTTCAAAAGTTGAGCTCTCTGATCTGGAGTGGAAAATGTGTTTATATTCCACGGAATACAGTGAAATTGGGCTAGTTCGGTTAAGGAACACAGAAACTATGCTTAGTTTAAAAAGGTTTACAAGAGAGAAGGTCAAAAGGCTGGGACTTGAAGCACAAGTTTTGAAGGAAAAAAATCTTATGAAGAGCATCAGTTCTTCAGCTTGCGTGCCGCAACTTCTCTGCACATGTTTTGATCAATCACATGCTGGCATATTGCTAAAGACATGCCTTGCTTGTCCTTTGTCTTCGATACTTCATGTCCCACTCGATGAATCTTCTGCACGTTTCTTTGCAGCCTCTCTTGTTATGGCACTGGAAGATTTGCACAAGCTCGGTGTTCTCCACAGAGGAATCTCCCCTGATGTTCTAATGTTGGATCAAAATGGACGCATACAGTTAGTAGACTTCAGATTTGGGAAAAGAACTTTGGGCGAGAGAACATATACTATTTGCGGGATGGCCGACTTTTTAGCACCCGAGATAGTTCAAGGCAATGGTCATGGTTTTGCTGCTGACAGGTGGGCAGTGGGAGTCTTAATCTATTTCATGCTGAAAGGTGAAATGCCATTTGGATCGTGGCGACGAAGCGAGCTCGATACTTTTTCAAAGATTGCAAAAGGACAGTTAAGTCTTCCCCAAATTTTCAGTCCTGAAGCAATGGATCTCATCGTCAAGGTAATGCTAGTGATCAACAATCTCCTCTACCCTTTCCCATTCCCATGTACTTTGCTAAGCCAATCATATATAGTAATTTTCACCTTTTGTAATAAAAAATATGATCAGTTACTTGAAGTTGATGAGAAGAAAAGACTTGGAAACGAGAACCAAAACTCTGTTAGAAGTCATCCATGGTTTGATGGCGTTGATTGGAAGGGGATCCATGACGGTACCTTTCCCGTTCCTGAGACAATAACTTCTCGTGTAGCTCAATATTTGGAGAGCTACCCTGAAGATTGCAGTGTTTCTTTAATTAAACCACCTCAAGATCTTGAAGAACAGGATGCCCCCGATTGGATCAACGACTGGTAGAAGTACAACACCGACAAAACTCTCCATCAAAATATGTATGCAGGCTTTTTTCAGGTGGAGTCTGTCTTCTGCTGATCGGTTCCCGTTGTTCTCAGTCAACACGAATCGATAATTTGAGAAATCCTTCGATATAATGCAAGTAAGTTGCATCGTATCAAGTTCATGTGAAGATTAGGTTGGTGAATAGATGTTATTTTTCCCATTTTTGTCTGTTGAAAATTGATTTCTGTGAATACAGAGTAGGTATATATTATATATGTAAATGAAGTAGAGTTCATTTTTTTGCTCTACAGTAAAATTGTTACTTGTCTTGTGGAACCTTCTGTTACTGGCTGCTGATAATAAATCCATCTTCTCCAGCCAGTGGTTTTATTGTTGGTTTTCTAGAGTTCATTCAAACTTAATATATTGCCTCTCTCTTCCTTTTGATTTTGTG

Coding sequence (CDS)

ATGCCTCCATCTCCAGCTCACAGGAAGTCATGGGAAGAAGAAGCACATATTGAACGAGCATTGCACGACCATTTCCTCTTCAGAAAACTAACTGATTCTCAGTGCCAAGTTTTATTAGATTGCATGCAAAGAGTTGAGGTCACACCCGGGCAAATTGTAGTCGAACAAGGTGGCGAAGGTGACTGTTTTTATGTGGTTGGCAGTGGAGAATTTGAGGTCTTGGCAACCCAGGAAGAAAGCCACGGAGAGGTTCCTCGGATTCTGCAACACTATACAGCTGAGAAGCTCTCTTCCTTCGGGGAACTGGCTTTGATGTATAACAAACCACTTCAAGCCTCGGTACGTGCTGTGACAAGTGGGACACTTTGGGCTTTGAAAAGAGAGGACTTTCGTGGAATTCTTATATCAGAATTTTCTAACTTGTCATCTTTGAAATTGCTTCGATCTGTGGACCTTTTGTCGAAGTTAACAATCTTGCAATTGAGTCAAATTGCAGACTCCCTTTCTGAAGTTCACTTCTCAGATGGGGAGCTGATTGTTGATGGGACAGAAGACTTATGTTCACTGCACATTATTCAAAAGGGACAAGTGAGGATTACTTTTGATGCGGAGTTAATGAGCAGTTCAAATGTTTACAGCTTCAATTATGCCAGTCAAAGAGAGGACAATGCTATGCAGAGTGATAACGAGATCTCAGCTATTAGGAAAGAGGGAAGTTATTTTGGCGAATGGGCACTTCTTGGTGAGCGTATCGGCTTCTTACGTGCAGTTGCTGTGGGAGATGTTGTGTGTGCTATTTTAACAAAGGAAAAGTTTGAATCAGTTGTTGGCCCTTTACCAAATCTCTCTCAAGATGATCAAAAGGTGAAAGAACACTCTTCAAACTCTCTCCATCAATCTGCCAAAATTATGGATATTTCAGCTCTTTCAAAAGTTGAGCTCTCTGATCTGGAGTGGAAAATGTGTTTATATTCCACGGAATACAGTGAAATTGGGCTAGTTCGGTTAAGGAACACAGAAACTATGCTTAGTTTAAAAAGGTTTACAAGAGAGAAGGTCAAAAGGCTGGGACTTGAAGCACAAGTTTTGAAGGAAAAAAATCTTATGAAGAGCATCAGTTCTTCAGCTTGCGTGCCGCAACTTCTCTGCACATGTTTTGATCAATCACATGCTGGCATATTGCTAAAGACATGCCTTGCTTGTCCTTTGTCTTCGATACTTCATGTCCCACTCGATGAATCTTCTGCACGTTTCTTTGCAGCCTCTCTTGTTATGGCACTGGAAGATTTGCACAAGCTCGGTGTTCTCCACAGAGGAATCTCCCCTGATGTTCTAATGTTGGATCAAAATGGACGCATACAGTTAGTAGACTTCAGATTTGGGAAAAGAACTTTGGGCGAGAGAACATATACTATTTGCGGGATGGCCGACTTTTTAGCACCCGAGATAGTTCAAGGCAATGGTCATGGTTTTGCTGCTGACAGGTGGGCAGTGGGAGTCTTAATCTATTTCATGCTGAAAGGTGAAATGCCATTTGGATCGTGGCGACGAAGCGAGCTCGATACTTTTTCAAAGATTGCAAAAGGACAGTTAAGTCTTCCCCAAATTTTCAGTCCTGAAGCAATGGATCTCATCGTCAAGGTAATGCTAGTGATCAACAATCTCCTCTACCCTTTCCCATTCCCATGTACTTTGCTAAGCCAATCATATATAGTAATTTTCACCTTTTGTAATAAAAAATATGATCAGTTACTTGAAGTTGATGAGAAGAAAAGACTTGGAAACGAGAACCAAAACTCTGTTAGAAGTCATCCATGGTTTGATGGCGTTGATTGGAAGGGGATCCATGACGGTACCTTTCCCGTTCCTGAGACAATAACTTCTCGTGTAGCTCAATATTTGGAGAGCTACCCTGAAGATTGCAGTGTTTCTTTAATTAAACCACCTCAAGATCTTGAAGAACAGGATGCCCCCGATTGGATCAACGACTGGTAG

Protein sequence

MPPSPAHRKSWEEEAHIERALHDHFLFRKLTDSQCQVLLDCMQRVEVTPGQIVVEQGGEGDCFYVVGSGEFEVLATQEESHGEVPRILQHYTAEKLSSFGELALMYNKPLQASVRAVTSGTLWALKREDFRGILISEFSNLSSLKLLRSVDLLSKLTILQLSQIADSLSEVHFSDGELIVDGTEDLCSLHIIQKGQVRITFDAELMSSSNVYSFNYASQREDNAMQSDNEISAIRKEGSYFGEWALLGERIGFLRAVAVGDVVCAILTKEKFESVVGPLPNLSQDDQKVKEHSSNSLHQSAKIMDISALSKVELSDLEWKMCLYSTEYSEIGLVRLRNTETMLSLKRFTREKVKRLGLEAQVLKEKNLMKSISSSACVPQLLCTCFDQSHAGILLKTCLACPLSSILHVPLDESSARFFAASLVMALEDLHKLGVLHRGISPDVLMLDQNGRIQLVDFRFGKRTLGERTYTICGMADFLAPEIVQGNGHGFAADRWAVGVLIYFMLKGEMPFGSWRRSELDTFSKIAKGQLSLPQIFSPEAMDLIVKVMLVINNLLYPFPFPCTLLSQSYIVIFTFCNKKYDQLLEVDEKKRLGNENQNSVRSHPWFDGVDWKGIHDGTFPVPETITSRVAQYLESYPEDCSVSLIKPPQDLEEQDAPDWINDW
Homology
BLAST of Lsi06G008270 vs. ExPASy Swiss-Prot
Match: Q9SL76 (Protein phosphatase 2C and cyclic nucleotide-binding/kinase domain-containing protein OS=Arabidopsis thaliana OX=3702 GN=At2g20050/At2g20040 PE=2 SV=2)

HSP 1 Score: 775.8 bits (2002), Expect = 4.0e-223
Identity = 406/669 (60.69%), Postives = 492/669 (73.54%), Query Frame = 0

Query: 1    MPPSPAHRKSWEEEAHIERALHDHFLFRKLTDSQCQVLLDCMQRVEVTPGQIVVEQGGEG 60
            +PPSPAHRK+WEEEAHIER L DHFLFRKLTDSQCQVLLDCMQR+E  PG IVV+QGGEG
Sbjct: 466  VPPSPAHRKTWEEEAHIERVLRDHFLFRKLTDSQCQVLLDCMQRLEANPGDIVVKQGGEG 525

Query: 61   DCFYVVGSGEFEVLATQEESHGEVPRILQHYTAEKLSSFGELALMYNKPLQASVRAVTSG 120
            DCFYVVGSGEFEVLATQ+  +GEVPRILQ YTAEK SSFGELALM+NKPLQASVRAV  G
Sbjct: 526  DCFYVVGSGEFEVLATQDGKNGEVPRILQRYTAEKQSSFGELALMHNKPLQASVRAVDHG 585

Query: 121  TLWALKREDFRGILISEFSNLSSLKLLRSVDLLSKLTILQLSQIADSLSEVHFSDGELIV 180
            TLWALKREDFRGIL+SEFSNL+SLKLLRSVDLLS+LTILQLS +A+SLSE  FSDG+ IV
Sbjct: 586  TLWALKREDFRGILMSEFSNLASLKLLRSVDLLSRLTILQLSHVAESLSEACFSDGQTIV 645

Query: 181  DGTEDLCSLHIIQKGQVRITFDAELMSSSNVYSFNYASQREDNAMQSDNEISAIRKEGSY 240
               + L  L++IQKG+V+I+F  E++ S NV S       E + ++   E+S  + EGSY
Sbjct: 646  TKDQKLQGLYVIQKGRVKISFCTEVLESQNVSSLTTGITNEYDNLEIGTEVSIEKHEGSY 705

Query: 241  FGEWALLGERIGFLRAVAVGDVVCAILTKEKFESVVGPLPNLSQDDQKVKEHSSNSLHQS 300
            FGEWALLGE    L  VAVG+VVC +LTKE FES VGPL N+S D  K +  S     +S
Sbjct: 706  FGEWALLGELKDSLSVVAVGEVVCVVLTKENFESAVGPLTNISDDGPKTRHSSFELSKES 765

Query: 301  AKIMDISALSKVELSDLEWKMCLYSTEYSEIGLVRLRNTETMLSLKRFTREKVKRLGLEA 360
            AK+ D +AL+K  L+DLEW  CL +T+ SEIGLV L++ E +LSLKRF+++KVK+LG EA
Sbjct: 766  AKVTDTTALAKATLADLEWTTCLSTTDCSEIGLVHLKDKENLLSLKRFSKQKVKKLGKEA 825

Query: 361  QVLKEKNLMKS-ISSSACVPQLLCTCFDQSHAGILLKTCLACPLSSILHVPLDESSARFF 420
            QVLKE+NLMK+ I  SA VP++LCTC DQ+ A ILL T LACP+SS+LH PLDESS RF 
Sbjct: 826  QVLKERNLMKNVIKPSAIVPEILCTCVDQTFAAILLNTTLACPISSLLHSPLDESSVRFI 885

Query: 421  AASLVMALEDLHKLGVLHRGISPDVLMLDQNGRIQLVDFRFGKRTLGERTYTICGMADFL 480
              SLV A+ED+HK  +L RG SP++LMLDQ+G +Q+VDFRF K+  GERT+TICG AD+L
Sbjct: 886  TGSLVSAIEDIHKNEILFRGSSPELLMLDQSGYLQIVDFRFAKKLSGERTFTICGNADYL 945

Query: 481  APEIVQGNGHGFAADRWAVGVLIYFMLKGEMPFGSWRRSELDTFSKIAKGQLSLPQIFSP 540
            APEIVQG GHG+AAD WA+GVLIY+ML+GEMPFGSWR SELDTF KIAKGQL+ P++ S 
Sbjct: 946  APEIVQGKGHGYAADWWALGVLIYYMLEGEMPFGSWRESELDTFQKIAKGQLTFPRVLSS 1005

Query: 541  EAMDLIVKVMLVINNLLYPFPFPCTLLSQSYIVIFTFCNKKYDQLLEVDEKKRLGNE-NQ 600
            EA DLI K                                    LLEVDE  R G++   
Sbjct: 1006 EAEDLITK------------------------------------LLEVDENLRFGSQGGP 1065

Query: 601  NSVRSHPWFDGVDWKGIHDGTFPVPETITSRVAQYLES---YPEDCSVSLIKPPQDLEEQ 660
             S++ HPWF+G+ W+ I +  F VP+ I SR+  +LE+    P + S SL       E+Q
Sbjct: 1066 ESIKKHPWFNGLKWEAISNREFQVPQEIISRIHHHLENDNVLPLETSKSL----DTTEDQ 1094

Query: 661  DAPDWINDW 665
            DA +W+ +W
Sbjct: 1126 DAQNWLEEW 1094

BLAST of Lsi06G008270 vs. ExPASy Swiss-Prot
Match: P0C605 (cGMP-dependent protein kinase 1 OS=Mus musculus OX=10090 GN=Prkg1 PE=1 SV=1)

HSP 1 Score: 220.3 bits (560), Expect = 6.4e-56
Identity = 176/656 (26.83%), Postives = 303/656 (46.19%), Query Frame = 0

Query: 9   KSWEEEAHIERALHDHFLFRKLTDSQCQVLLDCMQRVEVTPGQIVVEQGGEGDCFYVVGS 68
           KS   +  I+ A+ D+   + L  SQ Q ++DCM  VE      ++++G  G   YV+  
Sbjct: 86  KSERSKDLIKEAILDNDFMKNLELSQIQEIVDCMYPVEYGKDSCIIKEGDVGSLVYVMED 145

Query: 69  GEFEVLATQEESHGEVPRILQHYTAEKLSSFGELALMYNKPLQASVRAVTSGTLWALKRE 128
           G+ EV  T+E         ++  T      FGELA++YN    A+V+ + +  LWA+ R+
Sbjct: 146 GKVEV--TKEG--------VKLCTMGPGKVFGELAILYNCTRTATVKTLVNVKLWAIDRQ 205

Query: 129 DFRGILISE--FSNLSSLKLLRSVDLLSKLTILQLSQIADSLSEVHFSDGELIVDGTEDL 188
            F+ I++      +   ++ L+SV     L    LS++AD L E H+ +GE I+      
Sbjct: 206 CFQTIMMRTGLIKHTEYMEFLKSVPTFQSLPDEILSKLADVLEETHYENGEYIIRQGARG 265

Query: 189 CSLHIIQKGQVRITFDAELMSSSNVYSFNYASQREDNAMQSDNEISAIRKEGSYFGEWAL 248
            +  II KGQV +T                   RED+  +    +  + K G +FGE AL
Sbjct: 266 DTFFIISKGQVNVT-------------------REDSPSEDPVFLRTLGK-GDWFGEKAL 325

Query: 249 LGERIGFLRAVAVGDVVCAILTKEKFESVVGPLPNLSQDDQKVKEHSSNSLHQSAKIMDI 308
            GE +     +A   V C ++ ++ F+ ++G L ++S    K  E +       A   + 
Sbjct: 326 QGEDVRTANVIAAEAVTCLVIDRDSFKHLIGGLDDVS---NKAYEDAEAKAKYEA---EA 385

Query: 309 SALSKVELSDLEWKMCLYSTEYSEIGLVRLRNTET-MLSLKRFTREKVKRLGLEAQVLKE 368
           +  + ++LSD      L    +  + LV+L++ E+   ++K   +  +     +  +  E
Sbjct: 386 AFFANLKLSDFNIIDTLGVGGFGRVELVQLKSEESKTFAMKILKKRHIVDTRQQEHIRSE 445

Query: 369 KNLMKSISSSACVPQLLCTCFDQSHAGILLKTCLACPLSSIL--HVPLDESSARFFAASL 428
           K +M+   S   V +L  T  D  +  +L++ CL   L +IL      ++S+ RF+ A +
Sbjct: 446 KQIMQGAHSDFIV-RLYRTFKDSKYLYMLMEACLGGELWTILRDRGSFEDSTTRFYTACV 505

Query: 429 VMALEDLHKLGVLHRGISPDVLMLDQNGRIQLVDFRFGKRT-LGERTYTICGMADFLAPE 488
           V A   LH  G+++R + P+ L+LD  G  +LVDF F K+   G++T+T CG  +++APE
Sbjct: 506 VEAFAYLHSKGIIYRDLKPENLILDHRGYAKLVDFGFAKKIGFGKKTWTFCGTPEYVAPE 565

Query: 489 IVQGNGHGFAADRWAVGVLIYFMLKGEMPFGSWRRSELDTFSKIAKG--QLSLPQIFSPE 548
           I+   GH  +AD W++G+L+Y +L G  PF       + T++ I +G   +  P+  +  
Sbjct: 566 IILNKGHDISADYWSLGILMYELLTGSPPFSG--PDPMKTYNIILRGIDMIEFPKKIAKN 625

Query: 549 AMDLIVKVMLVINNLLYPFPFPCTLLSQSYIVIFTFCNKKYDQLLEVDEKKRLGN--ENQ 608
           A +LI K                                    L   +  +RLGN     
Sbjct: 626 AANLIKK------------------------------------LCRDNPSERLGNLKNGV 662

Query: 609 NSVRSHPWFDGVDWKGIHDGTFP---VPETITSRVAQYLESYPEDCSVSLIKPPQD 652
             ++ H WF+G +W+G+  GT     +P   +       +S+PED      +PP D
Sbjct: 686 KDIQKHKWFEGFNWEGLRKGTLTPPIIPSVASPTDTSNFDSFPEDSD----EPPPD 662

BLAST of Lsi06G008270 vs. ExPASy Swiss-Prot
Match: Q61410 (cGMP-dependent protein kinase 2 OS=Mus musculus OX=10090 GN=Prkg2 PE=1 SV=1)

HSP 1 Score: 219.5 bits (558), Expect = 1.1e-55
Identity = 178/681 (26.14%), Postives = 312/681 (45.81%), Query Frame = 0

Query: 8   RKSWEEEAHIERALHDHFLFRKLTDSQCQVLLDCMQRVEVTPGQIVVEQGGEGDCFYVVG 67
           RK   E+  I  AL+ +   ++L   Q + +++CM   +++ G  V++QG  G+  +V+ 
Sbjct: 150 RKDSSEKKLITDALNKNQFLKRLDPQQIKDMVECMYGEKLSTGSYVIKQGEPGNHIFVLA 209

Query: 68  SGEFEVLATQEESHGEVPRILQHYTAEKLSSFGELALMYNKPLQASVRAVTSGTLWALKR 127
            G  EV    E+    +P           ++FGELA++YN    ASV+A+T+   WAL R
Sbjct: 210 EGRLEVF-QGEKLLSSIP---------MWTTFGELAILYNCTRTASVKAITNVKTWALDR 269

Query: 128 EDFRGIL--ISEFSNLSSLKLLRSVDLLSKLTILQLSQIADSLSEVHFSDGELIVDGTED 187
           E F+ I+   ++  +      LRSV LL  L   +L++I D L   ++  G+ I+   E+
Sbjct: 270 EVFQNIMRRTAQARDEEYRNFLRSVSLLKNLPEDKLTKIIDCLEVEYYDKGDYIIREGEE 329

Query: 188 LCSLHIIQKGQVRITFDAELMSSSNVYSFNYASQREDNAMQSDNEISAIRKEGSYFGEWA 247
             +  I+ KG+V++T   E                         ++    ++G YFGE A
Sbjct: 330 GSTFFILAKGKVKVTQSTE--------------------GHDQPQLIKTLQKGEYFGEKA 389

Query: 248 LLGERIGFLRAVA-VGDVVCAILTKEKFESVVGP-----------LPNLSQDDQKVKEHS 307
           L+ + +     +A   DV C ++ +E F   VG            +  L++DD+K     
Sbjct: 390 LISDDVRSANIIAEENDVACLVIDRETFNQTVGTFDELQKYLEGYVATLNRDDEKRHAKR 449

Query: 308 SNSLHQSAKIMDISALSKVE----------LSDLEWKMCLYSTEYSEIGLVRLRNTETML 367
           S S  + +K + +  +   E            +LE    L    +  + LV+++N     
Sbjct: 450 SMSSWKLSKALSLEMIQLKEKVARFSSTSPFQNLEIIATLGVGGFGRVELVKVKNENVAF 509

Query: 368 SLKRFTREKVKRLGLEAQVLKEKNLMKSISSSACVPQLLCTCFDQSHAGILLKTCLACPL 427
           ++K   ++ +     +  V  EK +++ + S   V +L  T  D  +  +LL+ CL   L
Sbjct: 510 AMKCIRKKHIVDTKQQEHVYSEKRILEELCSPFIV-KLYRTFKDNKYVYMLLEACLGGEL 569

Query: 428 SSIL--HVPLDESSARFFAASLVMALEDLHKLGVLHRGISPDVLMLDQNGRIQLVDFRFG 487
            SIL      DE +++F  A +  A + LH LG+++R + P+ L+LD +G ++LVDF F 
Sbjct: 570 WSILRDRGSFDEPTSKFCVACVTEAFDYLHLLGIIYRDLKPENLILDADGYLKLVDFGFA 629

Query: 488 KRT-LGERTYTICGMADFLAPEIVQGNGHGFAADRWAVGVLIYFMLKGEMPFGSWRRSEL 547
           K+   G++T+T CG  +++APE++   GH F+ D W++G+L+Y +L G  PF      ++
Sbjct: 630 KKIGSGQKTWTFCGTPEYVAPEVILNKGHDFSVDFWSLGILVYELLTGNPPFSG--IDQM 689

Query: 548 DTFSKIAKGQLSLPQIFSPEAMDLIVKVMLVINNLLYPFPFPCTLLSQSYIVIFTFCNKK 607
            T++ I KG          E MD                 FP  +  +   +I   C + 
Sbjct: 690 MTYNLILKG---------IEKMD-----------------FPRKITRRPEDLIRRLCRQ- 749

Query: 608 YDQLLEVDEKKRLGN--ENQNSVRSHPWFDGVDWKGIHDGTFPVP---ETITSRVAQYLE 657
                  +  +RLGN     N ++ H W +G +W+G+   + P P   E        Y +
Sbjct: 750 -------NPTERLGNLKNGINDIKKHRWLNGFNWEGLKARSLPSPLRRELSGPIDHSYFD 759

BLAST of Lsi06G008270 vs. ExPASy Swiss-Prot
Match: Q13237 (cGMP-dependent protein kinase 2 OS=Homo sapiens OX=9606 GN=PRKG2 PE=1 SV=1)

HSP 1 Score: 219.2 bits (557), Expect = 1.4e-55
Identity = 178/681 (26.14%), Postives = 310/681 (45.52%), Query Frame = 0

Query: 8   RKSWEEEAHIERALHDHFLFRKLTDSQCQVLLDCMQRVEVTPGQIVVEQGGEGDCFYVVG 67
           RK   E+  I  AL+ +   ++L   Q + +++CM       G  +++QG  G+  +V+ 
Sbjct: 150 RKDSSEKKLITDALNKNQFLKRLDPQQIKDMVECMYGRNYQQGSYIIKQGEPGNHIFVLA 209

Query: 68  SGEFEVLATQEESHGEVPRILQHYTAEKLSSFGELALMYNKPLQASVRAVTSGTLWALKR 127
            G  EV    E+    +P           ++FGELA++YN    ASV+A+T+   WAL R
Sbjct: 210 EGRLEVF-QGEKLLSSIP---------MWTTFGELAILYNCTRTASVKAITNVKTWALDR 269

Query: 128 EDFRGIL--ISEFSNLSSLKLLRSVDLLSKLTILQLSQIADSLSEVHFSDGELIVDGTED 187
           E F+ I+   ++  +      LRSV LL  L   +L++I D L   ++  G+ I+   E+
Sbjct: 270 EVFQNIMRRTAQARDEQYRNFLRSVSLLKNLPEDKLTKIIDCLEVEYYDKGDYIIREGEE 329

Query: 188 LCSLHIIQKGQVRITFDAELMSSSNVYSFNYASQREDNAMQSDNEISAIRKEGSYFGEWA 247
             +  I+ KG+V++T   E                         ++    ++G YFGE A
Sbjct: 330 GSTFFILAKGKVKVTQSTE--------------------GHDQPQLIKTLQKGEYFGEKA 389

Query: 248 LLGERIGFLRAVA-VGDVVCAILTKEKFESVVGP-----------LPNLSQDDQKVKEHS 307
           L+ + +     +A   DV C ++ +E F   VG            + NL++DD+K     
Sbjct: 390 LISDDVRSANIIAEENDVACLVIDRETFNQTVGTFEELQKYLEGYVANLNRDDEKRHAKR 449

Query: 308 SNSLHQSAKIMDISAL----------SKVELSDLEWKMCLYSTEYSEIGLVRLRNTETML 367
           S S  + +K + +  +          S     +LE    L    +  + LV+++N     
Sbjct: 450 SMSNWKLSKALSLEMIQLKEKVARFSSSSPFQNLEIIATLGVGGFGRVELVKVKNENVAF 509

Query: 368 SLKRFTREKVKRLGLEAQVLKEKNLMKSISSSACVPQLLCTCFDQSHAGILLKTCLACPL 427
           ++K   ++ +     +  V  EK +++ + S   V +L  T  D  +  +LL+ CL   L
Sbjct: 510 AMKCIRKKHIVDTKQQEHVYSEKRILEELCSPFIV-KLYRTFKDNKYVYMLLEACLGGEL 569

Query: 428 SSIL--HVPLDESSARFFAASLVMALEDLHKLGVLHRGISPDVLMLDQNGRIQLVDFRFG 487
            SIL      DE +++F  A +  A + LH+LG+++R + P+ L+LD  G ++LVDF F 
Sbjct: 570 WSILRDRGSFDEPTSKFCVACVTEAFDYLHRLGIIYRDLKPENLILDAEGYLKLVDFGFA 629

Query: 488 KRT-LGERTYTICGMADFLAPEIVQGNGHGFAADRWAVGVLIYFMLKGEMPFGSWRRSEL 547
           K+   G++T+T CG  +++APE++   GH F+ D W++G+L+Y +L G  PF      ++
Sbjct: 630 KKIGSGQKTWTFCGTPEYVAPEVILNKGHDFSVDFWSLGILVYELLTGNPPFSG--VDQM 689

Query: 548 DTFSKIAKGQLSLPQIFSPEAMDLIVKVMLVINNLLYPFPFPCTLLSQSYIVIFTFCNKK 607
            T++ I KG          E MD                 FP  +  +   +I   C + 
Sbjct: 690 MTYNLILKG---------IEKMD-----------------FPRKITRRPEDLIRRLCRQ- 749

Query: 608 YDQLLEVDEKKRLGN--ENQNSVRSHPWFDGVDWKGIHDGTFPVP---ETITSRVAQYLE 657
                  +  +RLGN     N ++ H W +G +W+G+   + P P   E        Y +
Sbjct: 750 -------NPTERLGNLKNGINDIKKHRWLNGFNWEGLKARSLPSPLQRELKGPIDHSYFD 759

BLAST of Lsi06G008270 vs. ExPASy Swiss-Prot
Match: Q64595 (cGMP-dependent protein kinase 2 OS=Rattus norvegicus OX=10116 GN=Prkg2 PE=1 SV=1)

HSP 1 Score: 218.4 bits (555), Expect = 2.4e-55
Identity = 178/681 (26.14%), Postives = 310/681 (45.52%), Query Frame = 0

Query: 8   RKSWEEEAHIERALHDHFLFRKLTDSQCQVLLDCMQRVEVTPGQIVVEQGGEGDCFYVVG 67
           RK   E+  I  AL+ +   ++L   Q + +++CM       G  +V+QG  G+  +V+ 
Sbjct: 150 RKDSSEKKLITDALNKNQFLKRLDPQQIKDMVECMYGRNYQQGSYIVKQGEPGNHIFVLA 209

Query: 68  SGEFEVLATQEESHGEVPRILQHYTAEKLSSFGELALMYNKPLQASVRAVTSGTLWALKR 127
            G  EV    E+    +P           ++FGELA++YN    ASV+A+T+   WAL R
Sbjct: 210 EGRLEVF-QGEKLLSSIP---------MWTTFGELAILYNCTRTASVKAITNVKTWALDR 269

Query: 128 EDFRGIL--ISEFSNLSSLKLLRSVDLLSKLTILQLSQIADSLSEVHFSDGELIVDGTED 187
           E F+ I+   ++  +      LRSV LL  L   +L++I D L   ++  G+ I+   E+
Sbjct: 270 EVFQNIMRRTAQARDEEYRNFLRSVSLLKNLPEDKLTKIIDCLEVEYYDKGDYIIREGEE 329

Query: 188 LCSLHIIQKGQVRITFDAELMSSSNVYSFNYASQREDNAMQSDNEISAIRKEGSYFGEWA 247
             +  I+ KG+V++T   E                         ++    ++G YFGE A
Sbjct: 330 GSTFFILAKGKVKVTQSTE--------------------GHDQPQLIKTLQKGEYFGEKA 389

Query: 248 LLGERIGFLRAVA-VGDVVCAILTKEKFESVVGP-----------LPNLSQDDQKVKEHS 307
           L+ + +     +A   DV C ++ +E F   VG            +  L++DD+K     
Sbjct: 390 LISDDVRSANIIAEENDVACLVIDRETFNQTVGTFDELQKYLEGYVATLNRDDEKRHAKR 449

Query: 308 SNSLHQSAKIMDISALSKVE----------LSDLEWKMCLYSTEYSEIGLVRLRNTETML 367
           S S  + +K + +  +   E            +LE    L    +  + LV+++N     
Sbjct: 450 SMSSWKLSKALSLEMIQLKEKVARFSSTSPFQNLEIIATLGVGGFGRVELVKVKNENIAF 509

Query: 368 SLKRFTREKVKRLGLEAQVLKEKNLMKSISSSACVPQLLCTCFDQSHAGILLKTCLACPL 427
           ++K   ++ +     +  V  EK +++ + S   V +L  T  D  +  +LL+ CL   L
Sbjct: 510 AMKCIRKKHIVDTKQQEHVYSEKRILEELCSPFIV-KLYRTFKDNKYVYMLLEACLGGEL 569

Query: 428 SSIL--HVPLDESSARFFAASLVMALEDLHKLGVLHRGISPDVLMLDQNGRIQLVDFRFG 487
            SIL      DE +++F  A +  A + LH+LG+++R + P+ L+LD +G ++LVDF F 
Sbjct: 570 WSILRDRGSFDEPTSKFCVACVTEAFDYLHRLGIIYRDLKPENLILDADGYLKLVDFGFA 629

Query: 488 KRT-LGERTYTICGMADFLAPEIVQGNGHGFAADRWAVGVLIYFMLKGEMPFGSWRRSEL 547
           K+   G++T+T CG  +++APE++   GH F+ D W++G+L+Y +L G  PF      ++
Sbjct: 630 KKIGSGQKTWTFCGTPEYVAPEVILNKGHDFSVDFWSLGILVYELLTGNPPFSG--IDQM 689

Query: 548 DTFSKIAKGQLSLPQIFSPEAMDLIVKVMLVINNLLYPFPFPCTLLSQSYIVIFTFCNKK 607
            T++ I KG          E MD                 FP  +  +   +I   C + 
Sbjct: 690 MTYNLILKG---------IEKMD-----------------FPRKITRRPEDLIRRLCRQ- 749

Query: 608 YDQLLEVDEKKRLGN--ENQNSVRSHPWFDGVDWKGIHDGTFPVP---ETITSRVAQYLE 657
                  +  +RLGN     N ++ H W +G +W+G+   + P P   E        Y +
Sbjct: 750 -------NPTERLGNLKNGINDIKKHRWLNGFNWEGLKARSLPSPLRRELSGPIDHSYFD 759

BLAST of Lsi06G008270 vs. ExPASy TrEMBL
Match: A0A5D3DYE9 (Protein-serine/threonine phosphatase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold403G00620 PE=3 SV=1)

HSP 1 Score: 1140.2 bits (2948), Expect = 0.0e+00
Identity = 581/664 (87.50%), Postives = 602/664 (90.66%), Query Frame = 0

Query: 1    MPPSPAHRKSWEEEAHIERALHDHFLFRKLTDSQCQVLLDCMQRVEVTPGQIVVEQGGEG 60
            +PPSPAHRKSWEEEAHIERALHDHFLFRKLTDSQCQVLLDCMQRVEV PGQIVVEQGGEG
Sbjct: 582  VPPSPAHRKSWEEEAHIERALHDHFLFRKLTDSQCQVLLDCMQRVEVIPGQIVVEQGGEG 641

Query: 61   DCFYVVGSGEFEVLATQEESHGEVPRILQHYTAEKLSSFGELALMYNKPLQASVRAVTSG 120
            DCFYVVGSGEFEVLATQEESHGEVPR+LQHYTAEKLSSFGELALMYNKPLQASVRAVTSG
Sbjct: 642  DCFYVVGSGEFEVLATQEESHGEVPRVLQHYTAEKLSSFGELALMYNKPLQASVRAVTSG 701

Query: 121  TLWALKREDFRGILISEFSNLSSLKLLRSVDLLSKLTILQLSQIADSLSEVHFSDGELIV 180
            TLWALKREDFRGILISEFSNLSSLKLLRSVDLLSKLTILQLS IAD LSEV FSDGELIV
Sbjct: 702  TLWALKREDFRGILISEFSNLSSLKLLRSVDLLSKLTILQLSHIADCLSEVQFSDGELIV 761

Query: 181  DGTEDLCSLHIIQKGQVRITFDAELMSSSNVYSFNYASQREDNAMQSDNEISAIRKEGSY 240
            DGTE  C+LHIIQKGQVRITFDAELMS+SNVYSFNY SQ+ED A QS NEISAIRKEGSY
Sbjct: 762  DGTEGSCALHIIQKGQVRITFDAELMSNSNVYSFNYVSQKEDGAAQSGNEISAIRKEGSY 821

Query: 241  FGEWALLGERIGFLRAVAVGDVVCAILTKEKFESVVGPLPNLSQDDQKVKEHSSNSLHQS 300
            FGEWALLGE IGFLRAVAVGDV+C ILTKEKFESVVG LP LSQDDQK KEHSSNSLHQS
Sbjct: 822  FGEWALLGEHIGFLRAVAVGDVLCTILTKEKFESVVGSLPKLSQDDQKAKEHSSNSLHQS 881

Query: 301  AKIMDISALSKVELSDLEWKMCLYSTEYSEIGLVRLRNTETMLSLKRFTREKVKRLGLEA 360
            AK +DISALSKVELSDLEWKMCL+STEYSEIGLV+LRNTETMLSLKRFTR+KVKRLGLEA
Sbjct: 882  AKFIDISALSKVELSDLEWKMCLFSTEYSEIGLVQLRNTETMLSLKRFTRQKVKRLGLEA 941

Query: 361  QVLKEKNLMKSISSSACVPQLLCTCFDQSHAGILLKTCLACPLSSILHVPLDESSARFFA 420
            QVLKEKNLMK+ISSSACVP+LLCTCFDQSHAGILLKTCLACPLSSILHVPLDESSARFFA
Sbjct: 942  QVLKEKNLMKTISSSACVPELLCTCFDQSHAGILLKTCLACPLSSILHVPLDESSARFFA 1001

Query: 421  ASLVMALEDLHKLGVLHRGISPDVLMLDQNGRIQLVDFRFGKRTLGERTYTICGMADFLA 480
            ASLVMALEDLHKLGVLHRGISPDVLMLDQ G IQLVDFRFGK+TLGERTYTICGMADFLA
Sbjct: 1002 ASLVMALEDLHKLGVLHRGISPDVLMLDQTGHIQLVDFRFGKKTLGERTYTICGMADFLA 1061

Query: 481  PEIVQGNGHGFAADRWAVGVLIYFMLKGEMPFGSWRRSELDTFSKIAKGQLSLPQIFSPE 540
            PEIVQGNGHGFAAD WA+GVLI+FMLK EMPFGSWR+SELDTFSKIAKGQLSLPQIFSPE
Sbjct: 1062 PEIVQGNGHGFAADWWALGVLIHFMLKCEMPFGSWRQSELDTFSKIAKGQLSLPQIFSPE 1121

Query: 541  AMDLIVKVMLVINNLLYPFPFPCTLLSQSYIVIFTFCNKKYDQLLEVDEKKRLGNENQNS 600
            A+DLI K                                    LLEVDEKKRLGNENQN+
Sbjct: 1122 AIDLITK------------------------------------LLEVDEKKRLGNENQNA 1181

Query: 601  VRSHPWFDGVDWKGIHDGTFPVPETITSRVAQYLESYPEDCSVSLIKPPQDLEEQDAPDW 660
            VRSH WFDGVDWKGI +GTFPVPETITSRVAQYLESYPE+CS SL KPPQDLEEQD P+W
Sbjct: 1182 VRSHTWFDGVDWKGIREGTFPVPETITSRVAQYLESYPENCSASLTKPPQDLEEQDVPEW 1209

Query: 661  INDW 665
            INDW
Sbjct: 1242 INDW 1209

BLAST of Lsi06G008270 vs. ExPASy TrEMBL
Match: A0A1S3CHI5 (Protein-serine/threonine phosphatase OS=Cucumis melo OX=3656 GN=LOC103500993 PE=3 SV=1)

HSP 1 Score: 1140.2 bits (2948), Expect = 0.0e+00
Identity = 581/664 (87.50%), Postives = 602/664 (90.66%), Query Frame = 0

Query: 1    MPPSPAHRKSWEEEAHIERALHDHFLFRKLTDSQCQVLLDCMQRVEVTPGQIVVEQGGEG 60
            +PPSPAHRKSWEEEAHIERALHDHFLFRKLTDSQCQVLLDCMQRVEV PGQIVVEQGGEG
Sbjct: 455  VPPSPAHRKSWEEEAHIERALHDHFLFRKLTDSQCQVLLDCMQRVEVIPGQIVVEQGGEG 514

Query: 61   DCFYVVGSGEFEVLATQEESHGEVPRILQHYTAEKLSSFGELALMYNKPLQASVRAVTSG 120
            DCFYVVGSGEFEVLATQEESHGEVPR+LQHYTAEKLSSFGELALMYNKPLQASVRAVTSG
Sbjct: 515  DCFYVVGSGEFEVLATQEESHGEVPRVLQHYTAEKLSSFGELALMYNKPLQASVRAVTSG 574

Query: 121  TLWALKREDFRGILISEFSNLSSLKLLRSVDLLSKLTILQLSQIADSLSEVHFSDGELIV 180
            TLWALKREDFRGILISEFSNLSSLKLLRSVDLLSKLTILQLS IAD LSEV FSDGELIV
Sbjct: 575  TLWALKREDFRGILISEFSNLSSLKLLRSVDLLSKLTILQLSHIADCLSEVQFSDGELIV 634

Query: 181  DGTEDLCSLHIIQKGQVRITFDAELMSSSNVYSFNYASQREDNAMQSDNEISAIRKEGSY 240
            DGTE  C+LHIIQKGQVRITFDAELMS+SNVYSFNY SQ+ED A QS NEISAIRKEGSY
Sbjct: 635  DGTEGSCALHIIQKGQVRITFDAELMSNSNVYSFNYVSQKEDGAAQSGNEISAIRKEGSY 694

Query: 241  FGEWALLGERIGFLRAVAVGDVVCAILTKEKFESVVGPLPNLSQDDQKVKEHSSNSLHQS 300
            FGEWALLGE IGFLRAVAVGDV+C ILTKEKFESVVG LP LSQDDQK KEHSSNSLHQS
Sbjct: 695  FGEWALLGEHIGFLRAVAVGDVLCTILTKEKFESVVGSLPKLSQDDQKAKEHSSNSLHQS 754

Query: 301  AKIMDISALSKVELSDLEWKMCLYSTEYSEIGLVRLRNTETMLSLKRFTREKVKRLGLEA 360
            AK +DISALSKVELSDLEWKMCL+STEYSEIGLV+LRNTETMLSLKRFTR+KVKRLGLEA
Sbjct: 755  AKFIDISALSKVELSDLEWKMCLFSTEYSEIGLVQLRNTETMLSLKRFTRQKVKRLGLEA 814

Query: 361  QVLKEKNLMKSISSSACVPQLLCTCFDQSHAGILLKTCLACPLSSILHVPLDESSARFFA 420
            QVLKEKNLMK+ISSSACVP+LLCTCFDQSHAGILLKTCLACPLSSILHVPLDESSARFFA
Sbjct: 815  QVLKEKNLMKTISSSACVPELLCTCFDQSHAGILLKTCLACPLSSILHVPLDESSARFFA 874

Query: 421  ASLVMALEDLHKLGVLHRGISPDVLMLDQNGRIQLVDFRFGKRTLGERTYTICGMADFLA 480
            ASLVMALEDLHKLGVLHRGISPDVLMLDQ G IQLVDFRFGK+TLGERTYTICGMADFLA
Sbjct: 875  ASLVMALEDLHKLGVLHRGISPDVLMLDQTGHIQLVDFRFGKKTLGERTYTICGMADFLA 934

Query: 481  PEIVQGNGHGFAADRWAVGVLIYFMLKGEMPFGSWRRSELDTFSKIAKGQLSLPQIFSPE 540
            PEIVQGNGHGFAAD WA+GVLI+FMLK EMPFGSWR+SELDTFSKIAKGQLSLPQIFSPE
Sbjct: 935  PEIVQGNGHGFAADWWALGVLIHFMLKCEMPFGSWRQSELDTFSKIAKGQLSLPQIFSPE 994

Query: 541  AMDLIVKVMLVINNLLYPFPFPCTLLSQSYIVIFTFCNKKYDQLLEVDEKKRLGNENQNS 600
            A+DLI K                                    LLEVDEKKRLGNENQN+
Sbjct: 995  AIDLITK------------------------------------LLEVDEKKRLGNENQNA 1054

Query: 601  VRSHPWFDGVDWKGIHDGTFPVPETITSRVAQYLESYPEDCSVSLIKPPQDLEEQDAPDW 660
            VRSH WFDGVDWKGI +GTFPVPETITSRVAQYLESYPE+CS SL KPPQDLEEQD P+W
Sbjct: 1055 VRSHTWFDGVDWKGIREGTFPVPETITSRVAQYLESYPENCSASLTKPPQDLEEQDVPEW 1082

Query: 661  INDW 665
            INDW
Sbjct: 1115 INDW 1082

BLAST of Lsi06G008270 vs. ExPASy TrEMBL
Match: A0A5A7TSX7 (Protein-serine/threonine phosphatase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold114G00370 PE=3 SV=1)

HSP 1 Score: 1135.6 bits (2936), Expect = 0.0e+00
Identity = 581/665 (87.37%), Postives = 602/665 (90.53%), Query Frame = 0

Query: 1    MPPSPAHRKSWEEEAHIERALHDHFLFRKLTDSQCQVLLDCMQRVEVTPGQIVVEQGGEG 60
            +PPSPAHRKSWEEEAHIERALHDHFLFRKLTDSQCQVLLDCMQRVEV PGQIVVEQGGEG
Sbjct: 582  VPPSPAHRKSWEEEAHIERALHDHFLFRKLTDSQCQVLLDCMQRVEVIPGQIVVEQGGEG 641

Query: 61   DCFYVVGSGEFEVLATQEESHGEVPRILQHYTAEKLSSFGELALMYNKPLQASVRAVTSG 120
            DCFYVVGSGEFEVLATQEESHGEVPR+LQHYTAEKLSSFGELALMYNKPLQASVRAVTSG
Sbjct: 642  DCFYVVGSGEFEVLATQEESHGEVPRVLQHYTAEKLSSFGELALMYNKPLQASVRAVTSG 701

Query: 121  TLWALKREDFRGILISEFSNLSSLKLLRSVDLLSKLTILQLSQIADSLSEVHFSDGELIV 180
            TLWALKREDFRGILISEFSNLSSLKLLRSVDLLSKLTILQLS IAD LSEV FSDGELIV
Sbjct: 702  TLWALKREDFRGILISEFSNLSSLKLLRSVDLLSKLTILQLSHIADCLSEVQFSDGELIV 761

Query: 181  DGTEDLCSLHIIQKGQVRITFDAELMSSSNVYSFNYASQREDNAMQSDNEISAIRKEGSY 240
            DGTE  C+LHIIQKGQVRITFDAELMS+SNVYSFNY SQ+ED A QS NEISAIRKEGSY
Sbjct: 762  DGTEGSCALHIIQKGQVRITFDAELMSNSNVYSFNYVSQKEDGAAQSGNEISAIRKEGSY 821

Query: 241  FGEWALLGERIGFLRAVAVGDVVCAILTKEKFESVVGPLPNLSQDDQKVKEHSSNSLHQS 300
            FGEWALLGE IGFLRAVAVGDV+C ILTKEKFESVVG LP LSQDDQK KEHSSNSLHQS
Sbjct: 822  FGEWALLGEHIGFLRAVAVGDVLCTILTKEKFESVVGSLPKLSQDDQKAKEHSSNSLHQS 881

Query: 301  AKIMDISALSKVELSDLEWKMCLYSTEYSEIGLVRLRNT-ETMLSLKRFTREKVKRLGLE 360
            AK +DISALSKVELSDLEWKMCL+STEYSEIGLV+LRNT ETMLSLKRFTR+KVKRLGLE
Sbjct: 882  AKFIDISALSKVELSDLEWKMCLFSTEYSEIGLVQLRNTAETMLSLKRFTRQKVKRLGLE 941

Query: 361  AQVLKEKNLMKSISSSACVPQLLCTCFDQSHAGILLKTCLACPLSSILHVPLDESSARFF 420
            AQVLKEKNLMK+ISSSACVP+LLCTCFDQSHAGILLKTCLACPLSSILHVPLDESSARFF
Sbjct: 942  AQVLKEKNLMKTISSSACVPELLCTCFDQSHAGILLKTCLACPLSSILHVPLDESSARFF 1001

Query: 421  AASLVMALEDLHKLGVLHRGISPDVLMLDQNGRIQLVDFRFGKRTLGERTYTICGMADFL 480
            AASLVMALEDLHKLGVLHRGISPDVLMLDQ G IQLVDFRFGK+TLGERTYTICGMADFL
Sbjct: 1002 AASLVMALEDLHKLGVLHRGISPDVLMLDQTGHIQLVDFRFGKKTLGERTYTICGMADFL 1061

Query: 481  APEIVQGNGHGFAADRWAVGVLIYFMLKGEMPFGSWRRSELDTFSKIAKGQLSLPQIFSP 540
            APEIVQGNGHGFAAD WA+GVLI+FMLK EMPFGSWR+SELDTFSKIAKGQLSLPQIFSP
Sbjct: 1062 APEIVQGNGHGFAADWWALGVLIHFMLKCEMPFGSWRQSELDTFSKIAKGQLSLPQIFSP 1121

Query: 541  EAMDLIVKVMLVINNLLYPFPFPCTLLSQSYIVIFTFCNKKYDQLLEVDEKKRLGNENQN 600
            EA+DLI K                                    LLEVDEKKRLGNENQN
Sbjct: 1122 EAIDLITK------------------------------------LLEVDEKKRLGNENQN 1181

Query: 601  SVRSHPWFDGVDWKGIHDGTFPVPETITSRVAQYLESYPEDCSVSLIKPPQDLEEQDAPD 660
            +VRSH WFDGVDWKGI +GTFPVPETITSRVAQYLESYPE+CS SL KPPQDLEEQD P+
Sbjct: 1182 AVRSHTWFDGVDWKGIREGTFPVPETITSRVAQYLESYPENCSASLTKPPQDLEEQDVPE 1210

Query: 661  WINDW 665
            WINDW
Sbjct: 1242 WINDW 1210

BLAST of Lsi06G008270 vs. ExPASy TrEMBL
Match: A0A1S3CJ42 (Protein-serine/threonine phosphatase OS=Cucumis melo OX=3656 GN=LOC103500993 PE=3 SV=1)

HSP 1 Score: 1135.6 bits (2936), Expect = 0.0e+00
Identity = 581/665 (87.37%), Postives = 602/665 (90.53%), Query Frame = 0

Query: 1    MPPSPAHRKSWEEEAHIERALHDHFLFRKLTDSQCQVLLDCMQRVEVTPGQIVVEQGGEG 60
            +PPSPAHRKSWEEEAHIERALHDHFLFRKLTDSQCQVLLDCMQRVEV PGQIVVEQGGEG
Sbjct: 455  VPPSPAHRKSWEEEAHIERALHDHFLFRKLTDSQCQVLLDCMQRVEVIPGQIVVEQGGEG 514

Query: 61   DCFYVVGSGEFEVLATQEESHGEVPRILQHYTAEKLSSFGELALMYNKPLQASVRAVTSG 120
            DCFYVVGSGEFEVLATQEESHGEVPR+LQHYTAEKLSSFGELALMYNKPLQASVRAVTSG
Sbjct: 515  DCFYVVGSGEFEVLATQEESHGEVPRVLQHYTAEKLSSFGELALMYNKPLQASVRAVTSG 574

Query: 121  TLWALKREDFRGILISEFSNLSSLKLLRSVDLLSKLTILQLSQIADSLSEVHFSDGELIV 180
            TLWALKREDFRGILISEFSNLSSLKLLRSVDLLSKLTILQLS IAD LSEV FSDGELIV
Sbjct: 575  TLWALKREDFRGILISEFSNLSSLKLLRSVDLLSKLTILQLSHIADCLSEVQFSDGELIV 634

Query: 181  DGTEDLCSLHIIQKGQVRITFDAELMSSSNVYSFNYASQREDNAMQSDNEISAIRKEGSY 240
            DGTE  C+LHIIQKGQVRITFDAELMS+SNVYSFNY SQ+ED A QS NEISAIRKEGSY
Sbjct: 635  DGTEGSCALHIIQKGQVRITFDAELMSNSNVYSFNYVSQKEDGAAQSGNEISAIRKEGSY 694

Query: 241  FGEWALLGERIGFLRAVAVGDVVCAILTKEKFESVVGPLPNLSQDDQKVKEHSSNSLHQS 300
            FGEWALLGE IGFLRAVAVGDV+C ILTKEKFESVVG LP LSQDDQK KEHSSNSLHQS
Sbjct: 695  FGEWALLGEHIGFLRAVAVGDVLCTILTKEKFESVVGSLPKLSQDDQKAKEHSSNSLHQS 754

Query: 301  AKIMDISALSKVELSDLEWKMCLYSTEYSEIGLVRLRNT-ETMLSLKRFTREKVKRLGLE 360
            AK +DISALSKVELSDLEWKMCL+STEYSEIGLV+LRNT ETMLSLKRFTR+KVKRLGLE
Sbjct: 755  AKFIDISALSKVELSDLEWKMCLFSTEYSEIGLVQLRNTAETMLSLKRFTRQKVKRLGLE 814

Query: 361  AQVLKEKNLMKSISSSACVPQLLCTCFDQSHAGILLKTCLACPLSSILHVPLDESSARFF 420
            AQVLKEKNLMK+ISSSACVP+LLCTCFDQSHAGILLKTCLACPLSSILHVPLDESSARFF
Sbjct: 815  AQVLKEKNLMKTISSSACVPELLCTCFDQSHAGILLKTCLACPLSSILHVPLDESSARFF 874

Query: 421  AASLVMALEDLHKLGVLHRGISPDVLMLDQNGRIQLVDFRFGKRTLGERTYTICGMADFL 480
            AASLVMALEDLHKLGVLHRGISPDVLMLDQ G IQLVDFRFGK+TLGERTYTICGMADFL
Sbjct: 875  AASLVMALEDLHKLGVLHRGISPDVLMLDQTGHIQLVDFRFGKKTLGERTYTICGMADFL 934

Query: 481  APEIVQGNGHGFAADRWAVGVLIYFMLKGEMPFGSWRRSELDTFSKIAKGQLSLPQIFSP 540
            APEIVQGNGHGFAAD WA+GVLI+FMLK EMPFGSWR+SELDTFSKIAKGQLSLPQIFSP
Sbjct: 935  APEIVQGNGHGFAADWWALGVLIHFMLKCEMPFGSWRQSELDTFSKIAKGQLSLPQIFSP 994

Query: 541  EAMDLIVKVMLVINNLLYPFPFPCTLLSQSYIVIFTFCNKKYDQLLEVDEKKRLGNENQN 600
            EA+DLI K                                    LLEVDEKKRLGNENQN
Sbjct: 995  EAIDLITK------------------------------------LLEVDEKKRLGNENQN 1054

Query: 601  SVRSHPWFDGVDWKGIHDGTFPVPETITSRVAQYLESYPEDCSVSLIKPPQDLEEQDAPD 660
            +VRSH WFDGVDWKGI +GTFPVPETITSRVAQYLESYPE+CS SL KPPQDLEEQD P+
Sbjct: 1055 AVRSHTWFDGVDWKGIREGTFPVPETITSRVAQYLESYPENCSASLTKPPQDLEEQDVPE 1083

Query: 661  WINDW 665
            WINDW
Sbjct: 1115 WINDW 1083

BLAST of Lsi06G008270 vs. ExPASy TrEMBL
Match: A0A6J1CV03 (Protein-serine/threonine phosphatase OS=Momordica charantia OX=3673 GN=LOC111014466 PE=3 SV=1)

HSP 1 Score: 1092.0 bits (2823), Expect = 0.0e+00
Identity = 556/662 (83.99%), Postives = 590/662 (89.12%), Query Frame = 0

Query: 3    PSPAHRKSWEEEAHIERALHDHFLFRKLTDSQCQVLLDCMQRVEVTPGQIVVEQGGEGDC 62
            PSPAHRK+WEEEAHIERALHDHFLFRKLTDSQCQVLLDCMQRVEV PGQIVVEQGGEGDC
Sbjct: 459  PSPAHRKTWEEEAHIERALHDHFLFRKLTDSQCQVLLDCMQRVEVLPGQIVVEQGGEGDC 518

Query: 63   FYVVGSGEFEVLATQEESHGEVPRILQHYTAEKLSSFGELALMYNKPLQASVRAVTSGTL 122
            FYVVGSGEFEVL TQEE++ EVPR+L HYTAEKLSSFGELALMYNKPLQASVRAVTSGTL
Sbjct: 519  FYVVGSGEFEVLTTQEENNLEVPRVLHHYTAEKLSSFGELALMYNKPLQASVRAVTSGTL 578

Query: 123  WALKREDFRGILISEFSNLSSLKLLRSVDLLSKLTILQLSQIADSLSEVHFSDGELIVDG 182
            WALKREDFRGILISEFSNLSSLKLLRSVDLLSKLTILQLS IAD LSEV FSDG+LIVDG
Sbjct: 579  WALKREDFRGILISEFSNLSSLKLLRSVDLLSKLTILQLSHIADCLSEVTFSDGQLIVDG 638

Query: 183  TEDLCSLHIIQKGQVRITFDAELMSSSNVYSFNYASQREDNAMQSDNEISAIRKEGSYFG 242
            TEDLC+L I+QKGQVRITFDAE+MSSSNVYSFNYA+++ED++ QS  EIS I+KEGSYFG
Sbjct: 639  TEDLCALXIVQKGQVRITFDAEIMSSSNVYSFNYATKKEDDSAQSGKEISVIKKEGSYFG 698

Query: 243  EWALLGERIGFLRAVAVGDVVCAILTKEKFESVVGPLPNLSQDDQKVKEHSSNSLHQSAK 302
            EWALLG+RIGFLRAVA+GD VCAILTKEKFESVVGPLP  SQD+Q+ KEHSS++LH SAK
Sbjct: 699  EWALLGDRIGFLRAVALGDAVCAILTKEKFESVVGPLPKPSQDNQRAKEHSSDNLHHSAK 758

Query: 303  IMDISALSKVELSDLEWKMCLYSTEYSEIGLVRLRNTETMLSLKRFTREKVKRLGLEAQV 362
            IMDISALSKVEL+DLEW  CLYSTEYSEIGLVRLRNTETMLSLKRFTR+KVKRLGLEAQV
Sbjct: 759  IMDISALSKVELTDLEWITCLYSTEYSEIGLVRLRNTETMLSLKRFTRQKVKRLGLEAQV 818

Query: 363  LKEKNLMKSISSSACVPQLLCTCFDQSHAGILLKTCLACPLSSILHVPLDESSARFFAAS 422
            LKEKNLMKSIS SAC+PQ LCTC D+SHAGILLKTCLACPLSSILHVPLDESSARF AAS
Sbjct: 819  LKEKNLMKSISPSACMPQFLCTCLDRSHAGILLKTCLACPLSSILHVPLDESSARFCAAS 878

Query: 423  LVMALEDLHKLGVLHRGISPDVLMLDQNGRIQLVDFRFGKRTLGERTYTICGMADFLAPE 482
            LV ALEDLHKLGVL+RGISPDVLMLDQ GRIQL+DFRFGK+T GERT TICG ADFLAPE
Sbjct: 879  LVTALEDLHKLGVLYRGISPDVLMLDQTGRIQLIDFRFGKKTSGERTNTICGTADFLAPE 938

Query: 483  IVQGNGHGFAADRWAVGVLIYFMLKGEMPFGSWRRSELDTFSKIAKGQLSLPQIFSPEAM 542
            IVQGNGHGF AD WA+GVLIYFMLKGEMPFGSWR+SELDTFSKIAKGQLSLPQIFSPEA+
Sbjct: 939  IVQGNGHGFTADWWALGVLIYFMLKGEMPFGSWRQSELDTFSKIAKGQLSLPQIFSPEAI 998

Query: 543  DLIVKVMLVINNLLYPFPFPCTLLSQSYIVIFTFCNKKYDQLLEVDEKKRLGNENQNSVR 602
            DLIVK                                    LLEVDEKKRLG+EN NSVR
Sbjct: 999  DLIVK------------------------------------LLEVDEKKRLGSENPNSVR 1058

Query: 603  SHPWFDGVDWKGIHDGTFPVPETITSRVAQYLESYPEDCSVSLIKPPQDLEEQDAPDWIN 662
             HPWF+GVDWKGIHDGTFPVP+ ITSRVAQYLESY EDCSVSL+KPPQD EEQDA DWIN
Sbjct: 1059 RHPWFNGVDWKGIHDGTFPVPDAITSRVAQYLESYAEDCSVSLVKPPQDPEEQDATDWIN 1084

Query: 663  DW 665
            DW
Sbjct: 1119 DW 1084

BLAST of Lsi06G008270 vs. NCBI nr
Match: XP_038878760.1 (protein phosphatase 2C and cyclic nucleotide-binding/kinase domain-containing protein isoform X2 [Benincasa hispida])

HSP 1 Score: 1144.4 bits (2959), Expect = 0.0e+00
Identity = 588/664 (88.55%), Postives = 605/664 (91.11%), Query Frame = 0

Query: 1    MPPSPAHRKSWEEEAHIERALHDHFLFRKLTDSQCQVLLDCMQRVEVTPGQIVVEQGGEG 60
            +PPSPAHRKSWEEEAHIE+ALHDHFLFRKLTDSQCQVLLDCMQRVEV PGQIVVEQGGEG
Sbjct: 455  VPPSPAHRKSWEEEAHIEQALHDHFLFRKLTDSQCQVLLDCMQRVEVIPGQIVVEQGGEG 514

Query: 61   DCFYVVGSGEFEVLATQEESHGEVPRILQHYTAEKLSSFGELALMYNKPLQASVRAVTSG 120
            DCFYVVGSGEFEVLATQEESHGEVPRILQHYTAEKLSSFGELALMYNKPLQASVRAVTSG
Sbjct: 515  DCFYVVGSGEFEVLATQEESHGEVPRILQHYTAEKLSSFGELALMYNKPLQASVRAVTSG 574

Query: 121  TLWALKREDFRGILISEFSNLSSLKLLRSVDLLSKLTILQLSQIADSLSEVHFSDGELIV 180
            TLWALKREDFRGILISEFSNLSSLKLLRSVDLLSKLTILQLSQIAD LSEVHFSDGELIV
Sbjct: 575  TLWALKREDFRGILISEFSNLSSLKLLRSVDLLSKLTILQLSQIADRLSEVHFSDGELIV 634

Query: 181  DGTEDLCSLHIIQKGQVRITFDAELMSSSNVYSFNYASQREDNAMQSDNEISAIRKEGSY 240
            DGTE L +LHIIQKGQVRITFDAELMSSSNVYSFNY SQ+ED+A QS NEIS IRKEGSY
Sbjct: 635  DGTEGLHALHIIQKGQVRITFDAELMSSSNVYSFNYPSQKEDDAAQSGNEISTIRKEGSY 694

Query: 241  FGEWALLGERIGFLRAVAVGDVVCAILTKEKFESVVGPLPNLSQDDQKVKEHSSNSLHQS 300
            FGEWALLGE IGFLRAVAVGDVVC ILTKEKFESVVGPLPNLSQDDQK KE SSN LHQS
Sbjct: 695  FGEWALLGEHIGFLRAVAVGDVVCGILTKEKFESVVGPLPNLSQDDQKAKEQSSNPLHQS 754

Query: 301  AKIMDISALSKVELSDLEWKMCLYSTEYSEIGLVRLRNTETMLSLKRFTREKVKRLGLEA 360
            AKIMDISALSKVELSDLEWKMCLYSTEYSEIGLVRLRNTETMLSLKRFTR+KVKRLGLEA
Sbjct: 755  AKIMDISALSKVELSDLEWKMCLYSTEYSEIGLVRLRNTETMLSLKRFTRQKVKRLGLEA 814

Query: 361  QVLKEKNLMKSISSSACVPQLLCTCFDQSHAGILLKTCLACPLSSILHVPLDESSARFFA 420
            QVLKEKNLMKSISSSACVPQLLCTCF+QSHAGILLKTCLACPLSSILHVPLDESSARFFA
Sbjct: 815  QVLKEKNLMKSISSSACVPQLLCTCFNQSHAGILLKTCLACPLSSILHVPLDESSARFFA 874

Query: 421  ASLVMALEDLHKLGVLHRGISPDVLMLDQNGRIQLVDFRFGKRTLGERTYTICGMADFLA 480
            ASLVMALEDLHKLGVLHRGISPDVLML++ G I+LVDFRFGKRTLGERTYTICG ADFLA
Sbjct: 875  ASLVMALEDLHKLGVLHRGISPDVLMLNKTGYIKLVDFRFGKRTLGERTYTICGTADFLA 934

Query: 481  PEIVQGNGHGFAADRWAVGVLIYFMLKGEMPFGSWRRSELDTFSKIAKGQLSLPQIFSPE 540
            PEIVQGNGHGFAAD WA+GVLIYFMLKGEMPFG  R+SELDTF KI KGQLSLPQIFSPE
Sbjct: 935  PEIVQGNGHGFAADWWALGVLIYFMLKGEMPFGLCRQSELDTFLKITKGQLSLPQIFSPE 994

Query: 541  AMDLIVKVMLVINNLLYPFPFPCTLLSQSYIVIFTFCNKKYDQLLEVDEKKRLGNENQNS 600
            A+DLIVK                                    LLEVDE KRLGNENQNS
Sbjct: 995  AIDLIVK------------------------------------LLEVDETKRLGNENQNS 1054

Query: 601  VRSHPWFDGVDWKGIHDGTFPVPETITSRVAQYLESYPEDCSVSLIKPPQDLEEQDAPDW 660
            VRSHPWFDGVDWKGIHDGTFPVP+TITSRVAQYLESYPEDCS SL+KPPQDLEEQDAP+W
Sbjct: 1055 VRSHPWFDGVDWKGIHDGTFPVPKTITSRVAQYLESYPEDCSGSLMKPPQDLEEQDAPEW 1082

Query: 661  INDW 665
            I+DW
Sbjct: 1115 IDDW 1082

BLAST of Lsi06G008270 vs. NCBI nr
Match: XP_008462694.1 (PREDICTED: protein phosphatase 2C and cyclic nucleotide-binding/kinase domain-containing protein isoform X2 [Cucumis melo])

HSP 1 Score: 1140.2 bits (2948), Expect = 0.0e+00
Identity = 581/664 (87.50%), Postives = 602/664 (90.66%), Query Frame = 0

Query: 1    MPPSPAHRKSWEEEAHIERALHDHFLFRKLTDSQCQVLLDCMQRVEVTPGQIVVEQGGEG 60
            +PPSPAHRKSWEEEAHIERALHDHFLFRKLTDSQCQVLLDCMQRVEV PGQIVVEQGGEG
Sbjct: 455  VPPSPAHRKSWEEEAHIERALHDHFLFRKLTDSQCQVLLDCMQRVEVIPGQIVVEQGGEG 514

Query: 61   DCFYVVGSGEFEVLATQEESHGEVPRILQHYTAEKLSSFGELALMYNKPLQASVRAVTSG 120
            DCFYVVGSGEFEVLATQEESHGEVPR+LQHYTAEKLSSFGELALMYNKPLQASVRAVTSG
Sbjct: 515  DCFYVVGSGEFEVLATQEESHGEVPRVLQHYTAEKLSSFGELALMYNKPLQASVRAVTSG 574

Query: 121  TLWALKREDFRGILISEFSNLSSLKLLRSVDLLSKLTILQLSQIADSLSEVHFSDGELIV 180
            TLWALKREDFRGILISEFSNLSSLKLLRSVDLLSKLTILQLS IAD LSEV FSDGELIV
Sbjct: 575  TLWALKREDFRGILISEFSNLSSLKLLRSVDLLSKLTILQLSHIADCLSEVQFSDGELIV 634

Query: 181  DGTEDLCSLHIIQKGQVRITFDAELMSSSNVYSFNYASQREDNAMQSDNEISAIRKEGSY 240
            DGTE  C+LHIIQKGQVRITFDAELMS+SNVYSFNY SQ+ED A QS NEISAIRKEGSY
Sbjct: 635  DGTEGSCALHIIQKGQVRITFDAELMSNSNVYSFNYVSQKEDGAAQSGNEISAIRKEGSY 694

Query: 241  FGEWALLGERIGFLRAVAVGDVVCAILTKEKFESVVGPLPNLSQDDQKVKEHSSNSLHQS 300
            FGEWALLGE IGFLRAVAVGDV+C ILTKEKFESVVG LP LSQDDQK KEHSSNSLHQS
Sbjct: 695  FGEWALLGEHIGFLRAVAVGDVLCTILTKEKFESVVGSLPKLSQDDQKAKEHSSNSLHQS 754

Query: 301  AKIMDISALSKVELSDLEWKMCLYSTEYSEIGLVRLRNTETMLSLKRFTREKVKRLGLEA 360
            AK +DISALSKVELSDLEWKMCL+STEYSEIGLV+LRNTETMLSLKRFTR+KVKRLGLEA
Sbjct: 755  AKFIDISALSKVELSDLEWKMCLFSTEYSEIGLVQLRNTETMLSLKRFTRQKVKRLGLEA 814

Query: 361  QVLKEKNLMKSISSSACVPQLLCTCFDQSHAGILLKTCLACPLSSILHVPLDESSARFFA 420
            QVLKEKNLMK+ISSSACVP+LLCTCFDQSHAGILLKTCLACPLSSILHVPLDESSARFFA
Sbjct: 815  QVLKEKNLMKTISSSACVPELLCTCFDQSHAGILLKTCLACPLSSILHVPLDESSARFFA 874

Query: 421  ASLVMALEDLHKLGVLHRGISPDVLMLDQNGRIQLVDFRFGKRTLGERTYTICGMADFLA 480
            ASLVMALEDLHKLGVLHRGISPDVLMLDQ G IQLVDFRFGK+TLGERTYTICGMADFLA
Sbjct: 875  ASLVMALEDLHKLGVLHRGISPDVLMLDQTGHIQLVDFRFGKKTLGERTYTICGMADFLA 934

Query: 481  PEIVQGNGHGFAADRWAVGVLIYFMLKGEMPFGSWRRSELDTFSKIAKGQLSLPQIFSPE 540
            PEIVQGNGHGFAAD WA+GVLI+FMLK EMPFGSWR+SELDTFSKIAKGQLSLPQIFSPE
Sbjct: 935  PEIVQGNGHGFAADWWALGVLIHFMLKCEMPFGSWRQSELDTFSKIAKGQLSLPQIFSPE 994

Query: 541  AMDLIVKVMLVINNLLYPFPFPCTLLSQSYIVIFTFCNKKYDQLLEVDEKKRLGNENQNS 600
            A+DLI K                                    LLEVDEKKRLGNENQN+
Sbjct: 995  AIDLITK------------------------------------LLEVDEKKRLGNENQNA 1054

Query: 601  VRSHPWFDGVDWKGIHDGTFPVPETITSRVAQYLESYPEDCSVSLIKPPQDLEEQDAPDW 660
            VRSH WFDGVDWKGI +GTFPVPETITSRVAQYLESYPE+CS SL KPPQDLEEQD P+W
Sbjct: 1055 VRSHTWFDGVDWKGIREGTFPVPETITSRVAQYLESYPENCSASLTKPPQDLEEQDVPEW 1082

Query: 661  INDW 665
            INDW
Sbjct: 1115 INDW 1082

BLAST of Lsi06G008270 vs. NCBI nr
Match: TYK28736.1 (protein phosphatase 2C and cyclic nucleotide-binding/kinase domain-containing protein isoform X2 [Cucumis melo var. makuwa])

HSP 1 Score: 1140.2 bits (2948), Expect = 0.0e+00
Identity = 581/664 (87.50%), Postives = 602/664 (90.66%), Query Frame = 0

Query: 1    MPPSPAHRKSWEEEAHIERALHDHFLFRKLTDSQCQVLLDCMQRVEVTPGQIVVEQGGEG 60
            +PPSPAHRKSWEEEAHIERALHDHFLFRKLTDSQCQVLLDCMQRVEV PGQIVVEQGGEG
Sbjct: 582  VPPSPAHRKSWEEEAHIERALHDHFLFRKLTDSQCQVLLDCMQRVEVIPGQIVVEQGGEG 641

Query: 61   DCFYVVGSGEFEVLATQEESHGEVPRILQHYTAEKLSSFGELALMYNKPLQASVRAVTSG 120
            DCFYVVGSGEFEVLATQEESHGEVPR+LQHYTAEKLSSFGELALMYNKPLQASVRAVTSG
Sbjct: 642  DCFYVVGSGEFEVLATQEESHGEVPRVLQHYTAEKLSSFGELALMYNKPLQASVRAVTSG 701

Query: 121  TLWALKREDFRGILISEFSNLSSLKLLRSVDLLSKLTILQLSQIADSLSEVHFSDGELIV 180
            TLWALKREDFRGILISEFSNLSSLKLLRSVDLLSKLTILQLS IAD LSEV FSDGELIV
Sbjct: 702  TLWALKREDFRGILISEFSNLSSLKLLRSVDLLSKLTILQLSHIADCLSEVQFSDGELIV 761

Query: 181  DGTEDLCSLHIIQKGQVRITFDAELMSSSNVYSFNYASQREDNAMQSDNEISAIRKEGSY 240
            DGTE  C+LHIIQKGQVRITFDAELMS+SNVYSFNY SQ+ED A QS NEISAIRKEGSY
Sbjct: 762  DGTEGSCALHIIQKGQVRITFDAELMSNSNVYSFNYVSQKEDGAAQSGNEISAIRKEGSY 821

Query: 241  FGEWALLGERIGFLRAVAVGDVVCAILTKEKFESVVGPLPNLSQDDQKVKEHSSNSLHQS 300
            FGEWALLGE IGFLRAVAVGDV+C ILTKEKFESVVG LP LSQDDQK KEHSSNSLHQS
Sbjct: 822  FGEWALLGEHIGFLRAVAVGDVLCTILTKEKFESVVGSLPKLSQDDQKAKEHSSNSLHQS 881

Query: 301  AKIMDISALSKVELSDLEWKMCLYSTEYSEIGLVRLRNTETMLSLKRFTREKVKRLGLEA 360
            AK +DISALSKVELSDLEWKMCL+STEYSEIGLV+LRNTETMLSLKRFTR+KVKRLGLEA
Sbjct: 882  AKFIDISALSKVELSDLEWKMCLFSTEYSEIGLVQLRNTETMLSLKRFTRQKVKRLGLEA 941

Query: 361  QVLKEKNLMKSISSSACVPQLLCTCFDQSHAGILLKTCLACPLSSILHVPLDESSARFFA 420
            QVLKEKNLMK+ISSSACVP+LLCTCFDQSHAGILLKTCLACPLSSILHVPLDESSARFFA
Sbjct: 942  QVLKEKNLMKTISSSACVPELLCTCFDQSHAGILLKTCLACPLSSILHVPLDESSARFFA 1001

Query: 421  ASLVMALEDLHKLGVLHRGISPDVLMLDQNGRIQLVDFRFGKRTLGERTYTICGMADFLA 480
            ASLVMALEDLHKLGVLHRGISPDVLMLDQ G IQLVDFRFGK+TLGERTYTICGMADFLA
Sbjct: 1002 ASLVMALEDLHKLGVLHRGISPDVLMLDQTGHIQLVDFRFGKKTLGERTYTICGMADFLA 1061

Query: 481  PEIVQGNGHGFAADRWAVGVLIYFMLKGEMPFGSWRRSELDTFSKIAKGQLSLPQIFSPE 540
            PEIVQGNGHGFAAD WA+GVLI+FMLK EMPFGSWR+SELDTFSKIAKGQLSLPQIFSPE
Sbjct: 1062 PEIVQGNGHGFAADWWALGVLIHFMLKCEMPFGSWRQSELDTFSKIAKGQLSLPQIFSPE 1121

Query: 541  AMDLIVKVMLVINNLLYPFPFPCTLLSQSYIVIFTFCNKKYDQLLEVDEKKRLGNENQNS 600
            A+DLI K                                    LLEVDEKKRLGNENQN+
Sbjct: 1122 AIDLITK------------------------------------LLEVDEKKRLGNENQNA 1181

Query: 601  VRSHPWFDGVDWKGIHDGTFPVPETITSRVAQYLESYPEDCSVSLIKPPQDLEEQDAPDW 660
            VRSH WFDGVDWKGI +GTFPVPETITSRVAQYLESYPE+CS SL KPPQDLEEQD P+W
Sbjct: 1182 VRSHTWFDGVDWKGIREGTFPVPETITSRVAQYLESYPENCSASLTKPPQDLEEQDVPEW 1209

Query: 661  INDW 665
            INDW
Sbjct: 1242 INDW 1209

BLAST of Lsi06G008270 vs. NCBI nr
Match: XP_038878756.1 (protein phosphatase 2C and cyclic nucleotide-binding/kinase domain-containing protein isoform X1 [Benincasa hispida] >XP_038878757.1 protein phosphatase 2C and cyclic nucleotide-binding/kinase domain-containing protein isoform X1 [Benincasa hispida] >XP_038878759.1 protein phosphatase 2C and cyclic nucleotide-binding/kinase domain-containing protein isoform X1 [Benincasa hispida])

HSP 1 Score: 1139.8 bits (2947), Expect = 0.0e+00
Identity = 588/665 (88.42%), Postives = 605/665 (90.98%), Query Frame = 0

Query: 1    MPPSPAHRKSWEEEAHIERALHDHFLFRKLTDSQCQVLLDCMQRVEVTPGQIVVEQGGEG 60
            +PPSPAHRKSWEEEAHIE+ALHDHFLFRKLTDSQCQVLLDCMQRVEV PGQIVVEQGGEG
Sbjct: 455  VPPSPAHRKSWEEEAHIEQALHDHFLFRKLTDSQCQVLLDCMQRVEVIPGQIVVEQGGEG 514

Query: 61   DCFYVVGSGEFEVLATQEESHGEVPRILQHYTAEKLSSFGELALMYNKPLQASVRAVTSG 120
            DCFYVVGSGEFEVLATQEESHGEVPRILQHYTAEKLSSFGELALMYNKPLQASVRAVTSG
Sbjct: 515  DCFYVVGSGEFEVLATQEESHGEVPRILQHYTAEKLSSFGELALMYNKPLQASVRAVTSG 574

Query: 121  TLWALKREDFRGILISEFSNLSSLKLLRSVDLLSKLTILQLSQIADSLSEVHFSDGELIV 180
            TLWALKREDFRGILISEFSNLSSLKLLRSVDLLSKLTILQLSQIAD LSEVHFSDGELIV
Sbjct: 575  TLWALKREDFRGILISEFSNLSSLKLLRSVDLLSKLTILQLSQIADRLSEVHFSDGELIV 634

Query: 181  DGTEDLCSLHIIQKGQVRITFDAELMSSSNVYSFNYASQREDNAMQSDNEISAIRKEGSY 240
            DGTE L +LHIIQKGQVRITFDAELMSSSNVYSFNY SQ+ED+A QS NEIS IRKEGSY
Sbjct: 635  DGTEGLHALHIIQKGQVRITFDAELMSSSNVYSFNYPSQKEDDAAQSGNEISTIRKEGSY 694

Query: 241  FGEWALLGERIGFLRAVAVGDVVCAILTKEKFESVVGPLPNLSQDDQKVKEHSSNSLHQS 300
            FGEWALLGE IGFLRAVAVGDVVC ILTKEKFESVVGPLPNLSQDDQK KE SSN LHQS
Sbjct: 695  FGEWALLGEHIGFLRAVAVGDVVCGILTKEKFESVVGPLPNLSQDDQKAKEQSSNPLHQS 754

Query: 301  AKIMDISALSKVELSDLEWKMCLYSTEYSEIGLVRLRNT-ETMLSLKRFTREKVKRLGLE 360
            AKIMDISALSKVELSDLEWKMCLYSTEYSEIGLVRLRNT ETMLSLKRFTR+KVKRLGLE
Sbjct: 755  AKIMDISALSKVELSDLEWKMCLYSTEYSEIGLVRLRNTAETMLSLKRFTRQKVKRLGLE 814

Query: 361  AQVLKEKNLMKSISSSACVPQLLCTCFDQSHAGILLKTCLACPLSSILHVPLDESSARFF 420
            AQVLKEKNLMKSISSSACVPQLLCTCF+QSHAGILLKTCLACPLSSILHVPLDESSARFF
Sbjct: 815  AQVLKEKNLMKSISSSACVPQLLCTCFNQSHAGILLKTCLACPLSSILHVPLDESSARFF 874

Query: 421  AASLVMALEDLHKLGVLHRGISPDVLMLDQNGRIQLVDFRFGKRTLGERTYTICGMADFL 480
            AASLVMALEDLHKLGVLHRGISPDVLML++ G I+LVDFRFGKRTLGERTYTICG ADFL
Sbjct: 875  AASLVMALEDLHKLGVLHRGISPDVLMLNKTGYIKLVDFRFGKRTLGERTYTICGTADFL 934

Query: 481  APEIVQGNGHGFAADRWAVGVLIYFMLKGEMPFGSWRRSELDTFSKIAKGQLSLPQIFSP 540
            APEIVQGNGHGFAAD WA+GVLIYFMLKGEMPFG  R+SELDTF KI KGQLSLPQIFSP
Sbjct: 935  APEIVQGNGHGFAADWWALGVLIYFMLKGEMPFGLCRQSELDTFLKITKGQLSLPQIFSP 994

Query: 541  EAMDLIVKVMLVINNLLYPFPFPCTLLSQSYIVIFTFCNKKYDQLLEVDEKKRLGNENQN 600
            EA+DLIVK                                    LLEVDE KRLGNENQN
Sbjct: 995  EAIDLIVK------------------------------------LLEVDETKRLGNENQN 1054

Query: 601  SVRSHPWFDGVDWKGIHDGTFPVPETITSRVAQYLESYPEDCSVSLIKPPQDLEEQDAPD 660
            SVRSHPWFDGVDWKGIHDGTFPVP+TITSRVAQYLESYPEDCS SL+KPPQDLEEQDAP+
Sbjct: 1055 SVRSHPWFDGVDWKGIHDGTFPVPKTITSRVAQYLESYPEDCSGSLMKPPQDLEEQDAPE 1083

Query: 661  WINDW 665
            WI+DW
Sbjct: 1115 WIDDW 1083

BLAST of Lsi06G008270 vs. NCBI nr
Match: KAA0046522.1 (protein phosphatase 2C and cyclic nucleotide-binding/kinase domain-containing protein isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 1135.6 bits (2936), Expect = 0.0e+00
Identity = 581/665 (87.37%), Postives = 602/665 (90.53%), Query Frame = 0

Query: 1    MPPSPAHRKSWEEEAHIERALHDHFLFRKLTDSQCQVLLDCMQRVEVTPGQIVVEQGGEG 60
            +PPSPAHRKSWEEEAHIERALHDHFLFRKLTDSQCQVLLDCMQRVEV PGQIVVEQGGEG
Sbjct: 582  VPPSPAHRKSWEEEAHIERALHDHFLFRKLTDSQCQVLLDCMQRVEVIPGQIVVEQGGEG 641

Query: 61   DCFYVVGSGEFEVLATQEESHGEVPRILQHYTAEKLSSFGELALMYNKPLQASVRAVTSG 120
            DCFYVVGSGEFEVLATQEESHGEVPR+LQHYTAEKLSSFGELALMYNKPLQASVRAVTSG
Sbjct: 642  DCFYVVGSGEFEVLATQEESHGEVPRVLQHYTAEKLSSFGELALMYNKPLQASVRAVTSG 701

Query: 121  TLWALKREDFRGILISEFSNLSSLKLLRSVDLLSKLTILQLSQIADSLSEVHFSDGELIV 180
            TLWALKREDFRGILISEFSNLSSLKLLRSVDLLSKLTILQLS IAD LSEV FSDGELIV
Sbjct: 702  TLWALKREDFRGILISEFSNLSSLKLLRSVDLLSKLTILQLSHIADCLSEVQFSDGELIV 761

Query: 181  DGTEDLCSLHIIQKGQVRITFDAELMSSSNVYSFNYASQREDNAMQSDNEISAIRKEGSY 240
            DGTE  C+LHIIQKGQVRITFDAELMS+SNVYSFNY SQ+ED A QS NEISAIRKEGSY
Sbjct: 762  DGTEGSCALHIIQKGQVRITFDAELMSNSNVYSFNYVSQKEDGAAQSGNEISAIRKEGSY 821

Query: 241  FGEWALLGERIGFLRAVAVGDVVCAILTKEKFESVVGPLPNLSQDDQKVKEHSSNSLHQS 300
            FGEWALLGE IGFLRAVAVGDV+C ILTKEKFESVVG LP LSQDDQK KEHSSNSLHQS
Sbjct: 822  FGEWALLGEHIGFLRAVAVGDVLCTILTKEKFESVVGSLPKLSQDDQKAKEHSSNSLHQS 881

Query: 301  AKIMDISALSKVELSDLEWKMCLYSTEYSEIGLVRLRNT-ETMLSLKRFTREKVKRLGLE 360
            AK +DISALSKVELSDLEWKMCL+STEYSEIGLV+LRNT ETMLSLKRFTR+KVKRLGLE
Sbjct: 882  AKFIDISALSKVELSDLEWKMCLFSTEYSEIGLVQLRNTAETMLSLKRFTRQKVKRLGLE 941

Query: 361  AQVLKEKNLMKSISSSACVPQLLCTCFDQSHAGILLKTCLACPLSSILHVPLDESSARFF 420
            AQVLKEKNLMK+ISSSACVP+LLCTCFDQSHAGILLKTCLACPLSSILHVPLDESSARFF
Sbjct: 942  AQVLKEKNLMKTISSSACVPELLCTCFDQSHAGILLKTCLACPLSSILHVPLDESSARFF 1001

Query: 421  AASLVMALEDLHKLGVLHRGISPDVLMLDQNGRIQLVDFRFGKRTLGERTYTICGMADFL 480
            AASLVMALEDLHKLGVLHRGISPDVLMLDQ G IQLVDFRFGK+TLGERTYTICGMADFL
Sbjct: 1002 AASLVMALEDLHKLGVLHRGISPDVLMLDQTGHIQLVDFRFGKKTLGERTYTICGMADFL 1061

Query: 481  APEIVQGNGHGFAADRWAVGVLIYFMLKGEMPFGSWRRSELDTFSKIAKGQLSLPQIFSP 540
            APEIVQGNGHGFAAD WA+GVLI+FMLK EMPFGSWR+SELDTFSKIAKGQLSLPQIFSP
Sbjct: 1062 APEIVQGNGHGFAADWWALGVLIHFMLKCEMPFGSWRQSELDTFSKIAKGQLSLPQIFSP 1121

Query: 541  EAMDLIVKVMLVINNLLYPFPFPCTLLSQSYIVIFTFCNKKYDQLLEVDEKKRLGNENQN 600
            EA+DLI K                                    LLEVDEKKRLGNENQN
Sbjct: 1122 EAIDLITK------------------------------------LLEVDEKKRLGNENQN 1181

Query: 601  SVRSHPWFDGVDWKGIHDGTFPVPETITSRVAQYLESYPEDCSVSLIKPPQDLEEQDAPD 660
            +VRSH WFDGVDWKGI +GTFPVPETITSRVAQYLESYPE+CS SL KPPQDLEEQD P+
Sbjct: 1182 AVRSHTWFDGVDWKGIREGTFPVPETITSRVAQYLESYPENCSASLTKPPQDLEEQDVPE 1210

Query: 661  WINDW 665
            WINDW
Sbjct: 1242 WINDW 1210

BLAST of Lsi06G008270 vs. TAIR 10
Match: AT2G20050.1 (protein serine/threonine phosphatases;protein kinases;catalytics;cAMP-dependent protein kinase regulators;ATP binding;protein serine/threonine phosphatases )

HSP 1 Score: 775.8 bits (2002), Expect = 2.8e-224
Identity = 406/669 (60.69%), Postives = 492/669 (73.54%), Query Frame = 0

Query: 1    MPPSPAHRKSWEEEAHIERALHDHFLFRKLTDSQCQVLLDCMQRVEVTPGQIVVEQGGEG 60
            +PPSPAHRK+WEEEAHIER L DHFLFRKLTDSQCQVLLDCMQR+E  PG IVV+QGGEG
Sbjct: 466  VPPSPAHRKTWEEEAHIERVLRDHFLFRKLTDSQCQVLLDCMQRLEANPGDIVVKQGGEG 525

Query: 61   DCFYVVGSGEFEVLATQEESHGEVPRILQHYTAEKLSSFGELALMYNKPLQASVRAVTSG 120
            DCFYVVGSGEFEVLATQ+  +GEVPRILQ YTAEK SSFGELALM+NKPLQASVRAV  G
Sbjct: 526  DCFYVVGSGEFEVLATQDGKNGEVPRILQRYTAEKQSSFGELALMHNKPLQASVRAVDHG 585

Query: 121  TLWALKREDFRGILISEFSNLSSLKLLRSVDLLSKLTILQLSQIADSLSEVHFSDGELIV 180
            TLWALKREDFRGIL+SEFSNL+SLKLLRSVDLLS+LTILQLS +A+SLSE  FSDG+ IV
Sbjct: 586  TLWALKREDFRGILMSEFSNLASLKLLRSVDLLSRLTILQLSHVAESLSEACFSDGQTIV 645

Query: 181  DGTEDLCSLHIIQKGQVRITFDAELMSSSNVYSFNYASQREDNAMQSDNEISAIRKEGSY 240
               + L  L++IQKG+V+I+F  E++ S NV S       E + ++   E+S  + EGSY
Sbjct: 646  TKDQKLQGLYVIQKGRVKISFCTEVLESQNVSSLTTGITNEYDNLEIGTEVSIEKHEGSY 705

Query: 241  FGEWALLGERIGFLRAVAVGDVVCAILTKEKFESVVGPLPNLSQDDQKVKEHSSNSLHQS 300
            FGEWALLGE    L  VAVG+VVC +LTKE FES VGPL N+S D  K +  S     +S
Sbjct: 706  FGEWALLGELKDSLSVVAVGEVVCVVLTKENFESAVGPLTNISDDGPKTRHSSFELSKES 765

Query: 301  AKIMDISALSKVELSDLEWKMCLYSTEYSEIGLVRLRNTETMLSLKRFTREKVKRLGLEA 360
            AK+ D +AL+K  L+DLEW  CL +T+ SEIGLV L++ E +LSLKRF+++KVK+LG EA
Sbjct: 766  AKVTDTTALAKATLADLEWTTCLSTTDCSEIGLVHLKDKENLLSLKRFSKQKVKKLGKEA 825

Query: 361  QVLKEKNLMKS-ISSSACVPQLLCTCFDQSHAGILLKTCLACPLSSILHVPLDESSARFF 420
            QVLKE+NLMK+ I  SA VP++LCTC DQ+ A ILL T LACP+SS+LH PLDESS RF 
Sbjct: 826  QVLKERNLMKNVIKPSAIVPEILCTCVDQTFAAILLNTTLACPISSLLHSPLDESSVRFI 885

Query: 421  AASLVMALEDLHKLGVLHRGISPDVLMLDQNGRIQLVDFRFGKRTLGERTYTICGMADFL 480
              SLV A+ED+HK  +L RG SP++LMLDQ+G +Q+VDFRF K+  GERT+TICG AD+L
Sbjct: 886  TGSLVSAIEDIHKNEILFRGSSPELLMLDQSGYLQIVDFRFAKKLSGERTFTICGNADYL 945

Query: 481  APEIVQGNGHGFAADRWAVGVLIYFMLKGEMPFGSWRRSELDTFSKIAKGQLSLPQIFSP 540
            APEIVQG GHG+AAD WA+GVLIY+ML+GEMPFGSWR SELDTF KIAKGQL+ P++ S 
Sbjct: 946  APEIVQGKGHGYAADWWALGVLIYYMLEGEMPFGSWRESELDTFQKIAKGQLTFPRVLSS 1005

Query: 541  EAMDLIVKVMLVINNLLYPFPFPCTLLSQSYIVIFTFCNKKYDQLLEVDEKKRLGNE-NQ 600
            EA DLI K                                    LLEVDE  R G++   
Sbjct: 1006 EAEDLITK------------------------------------LLEVDENLRFGSQGGP 1065

Query: 601  NSVRSHPWFDGVDWKGIHDGTFPVPETITSRVAQYLES---YPEDCSVSLIKPPQDLEEQ 660
             S++ HPWF+G+ W+ I +  F VP+ I SR+  +LE+    P + S SL       E+Q
Sbjct: 1066 ESIKKHPWFNGLKWEAISNREFQVPQEIISRIHHHLENDNVLPLETSKSL----DTTEDQ 1094

Query: 661  DAPDWINDW 665
            DA +W+ +W
Sbjct: 1126 DAQNWLEEW 1094

BLAST of Lsi06G008270 vs. TAIR 10
Match: AT2G20050.2 (protein serine/threonine phosphatases;protein kinases;catalytics;cAMP-dependent protein kinase regulators;ATP binding;protein serine/threonine phosphatases )

HSP 1 Score: 770.8 bits (1989), Expect = 9.1e-223
Identity = 406/669 (60.69%), Postives = 491/669 (73.39%), Query Frame = 0

Query: 1    MPPSPAHRKSWEEEAHIERALHDHFLFRKLTDSQCQVLLDCMQRVEVTPGQIVVEQGGEG 60
            +PPSPAHRK+WEEEAHIER L DHFLFRKLTDSQCQVLLDCMQR+E  PG IVV+QGGEG
Sbjct: 466  VPPSPAHRKTWEEEAHIERVLRDHFLFRKLTDSQCQVLLDCMQRLEANPGDIVVKQGGEG 525

Query: 61   DCFYVVGSGEFEVLATQEESHGEVPRILQHYTAEKLSSFGELALMYNKPLQASVRAVTSG 120
            DCFYVVGSGEFEVLATQ   +GEVPRILQ YTAEK SSFGELALM+NKPLQASVRAV  G
Sbjct: 526  DCFYVVGSGEFEVLATQ---NGEVPRILQRYTAEKQSSFGELALMHNKPLQASVRAVDHG 585

Query: 121  TLWALKREDFRGILISEFSNLSSLKLLRSVDLLSKLTILQLSQIADSLSEVHFSDGELIV 180
            TLWALKREDFRGIL+SEFSNL+SLKLLRSVDLLS+LTILQLS +A+SLSE  FSDG+ IV
Sbjct: 586  TLWALKREDFRGILMSEFSNLASLKLLRSVDLLSRLTILQLSHVAESLSEACFSDGQTIV 645

Query: 181  DGTEDLCSLHIIQKGQVRITFDAELMSSSNVYSFNYASQREDNAMQSDNEISAIRKEGSY 240
               + L  L++IQKG+V+I+F  E++ S NV S       E + ++   E+S  + EGSY
Sbjct: 646  TKDQKLQGLYVIQKGRVKISFCTEVLESQNVSSLTTGITNEYDNLEIGTEVSIEKHEGSY 705

Query: 241  FGEWALLGERIGFLRAVAVGDVVCAILTKEKFESVVGPLPNLSQDDQKVKEHSSNSLHQS 300
            FGEWALLGE    L  VAVG+VVC +LTKE FES VGPL N+S D  K +  S     +S
Sbjct: 706  FGEWALLGELKDSLSVVAVGEVVCVVLTKENFESAVGPLTNISDDGPKTRHSSFELSKES 765

Query: 301  AKIMDISALSKVELSDLEWKMCLYSTEYSEIGLVRLRNTETMLSLKRFTREKVKRLGLEA 360
            AK+ D +AL+K  L+DLEW  CL +T+ SEIGLV L++ E +LSLKRF+++KVK+LG EA
Sbjct: 766  AKVTDTTALAKATLADLEWTTCLSTTDCSEIGLVHLKDKENLLSLKRFSKQKVKKLGKEA 825

Query: 361  QVLKEKNLMKS-ISSSACVPQLLCTCFDQSHAGILLKTCLACPLSSILHVPLDESSARFF 420
            QVLKE+NLMK+ I  SA VP++LCTC DQ+ A ILL T LACP+SS+LH PLDESS RF 
Sbjct: 826  QVLKERNLMKNVIKPSAIVPEILCTCVDQTFAAILLNTTLACPISSLLHSPLDESSVRFI 885

Query: 421  AASLVMALEDLHKLGVLHRGISPDVLMLDQNGRIQLVDFRFGKRTLGERTYTICGMADFL 480
              SLV A+ED+HK  +L RG SP++LMLDQ+G +Q+VDFRF K+  GERT+TICG AD+L
Sbjct: 886  TGSLVSAIEDIHKNEILFRGSSPELLMLDQSGYLQIVDFRFAKKLSGERTFTICGNADYL 945

Query: 481  APEIVQGNGHGFAADRWAVGVLIYFMLKGEMPFGSWRRSELDTFSKIAKGQLSLPQIFSP 540
            APEIVQG GHG+AAD WA+GVLIY+ML+GEMPFGSWR SELDTF KIAKGQL+ P++ S 
Sbjct: 946  APEIVQGKGHGYAADWWALGVLIYYMLEGEMPFGSWRESELDTFQKIAKGQLTFPRVLSS 1005

Query: 541  EAMDLIVKVMLVINNLLYPFPFPCTLLSQSYIVIFTFCNKKYDQLLEVDEKKRLGNE-NQ 600
            EA DLI K                                    LLEVDE  R G++   
Sbjct: 1006 EAEDLITK------------------------------------LLEVDENLRFGSQGGP 1065

Query: 601  NSVRSHPWFDGVDWKGIHDGTFPVPETITSRVAQYLES---YPEDCSVSLIKPPQDLEEQ 660
             S++ HPWF+G+ W+ I +  F VP+ I SR+  +LE+    P + S SL       E+Q
Sbjct: 1066 ESIKKHPWFNGLKWEAISNREFQVPQEIISRIHHHLENDNVLPLETSKSL----DTTEDQ 1091

Query: 661  DAPDWINDW 665
            DA +W+ +W
Sbjct: 1126 DAQNWLEEW 1091

BLAST of Lsi06G008270 vs. TAIR 10
Match: AT1G45160.1 (Protein kinase superfamily protein )

HSP 1 Score: 114.0 bits (284), Expect = 4.6e-25
Identity = 101/391 (25.83%), Postives = 168/391 (42.97%), Query Frame = 0

Query: 291 EHSSNSLHQSAKIMDISALSKVELSDLEWKMCLYSTEYSEIGLVRLRNTETMLSLKRFTR 350
           + S + L  ++    +    ++ + D E    +    + ++ L R R T    ++K   +
Sbjct: 644 KESEDVLEHASATPQLLLKDRISIDDFEIIKPISRGAFGKVFLARKRTTGDFFAIKVLKK 703

Query: 351 EKVKRLGLEAQVLKEKNLMKSISSSACVPQLLC-TCFDQSHAGILLKTCLACPLSSILHV 410
             + R     ++L+E+N++ ++     V      TC D  +  ++++      L S+L  
Sbjct: 704 LDMIRKNDIERILQERNILITVRYPFLVRFFYSFTCRDNLY--LVMEYLNGGDLYSLLQK 763

Query: 411 P--LDESSARFFAASLVMALEDLHKLGVLHRGISPDVLMLDQNGRIQLVDFRFGKRTLGE 470
              LDE  AR + A LV+ALE LH L ++HR + PD L++  NG I+L DF   K  L  
Sbjct: 764 VGCLDEEIARIYIAELVLALEYLHSLKIVHRDLKPDNLLIAYNGHIKLTDFGLSKIGLIN 823

Query: 471 RT-----------------------------YTICGMADFLAPEIVQGNGHGFAADRWAV 530
            T                             ++  G  D+LAPEI+ G  HG+AAD W+ 
Sbjct: 824 NTIDLSGHESDVSPRTNSHHFQKNQEEERIRHSAVGTPDYLAPEILLGTEHGYAADWWSA 883

Query: 531 GVLIYFMLKGEMPFGSWRRSELDTFSKIAKGQLSLPQI---FSPEAMDLIVKVMLVINNL 590
           G++++ +L G  PF + R  ++  F  I  G++  P +    S EA DLI          
Sbjct: 884 GIVLFELLTGIPPFTASRPEKI--FDNILNGKMPWPDVPGEMSYEAQDLI---------- 943

Query: 591 LYPFPFPCTLLSQSYIVIFTFCNKKYDQLLEVDEKKRLGNENQNSVRSHPWFDGVDWKGI 641
                                     ++LL  + +KRLG      V+SHP+F GVDW+ +
Sbjct: 944 --------------------------NRLLVHEPEKRLGANGAAEVKSHPFFQGVDWENL 994

BLAST of Lsi06G008270 vs. TAIR 10
Match: AT1G45160.2 (Protein kinase superfamily protein )

HSP 1 Score: 114.0 bits (284), Expect = 4.6e-25
Identity = 101/391 (25.83%), Postives = 168/391 (42.97%), Query Frame = 0

Query: 291 EHSSNSLHQSAKIMDISALSKVELSDLEWKMCLYSTEYSEIGLVRLRNTETMLSLKRFTR 350
           + S + L  ++    +    ++ + D E    +    + ++ L R R T    ++K   +
Sbjct: 644 KESEDVLEHASATPQLLLKDRISIDDFEIIKPISRGAFGKVFLARKRTTGDFFAIKVLKK 703

Query: 351 EKVKRLGLEAQVLKEKNLMKSISSSACVPQLLC-TCFDQSHAGILLKTCLACPLSSILHV 410
             + R     ++L+E+N++ ++     V      TC D  +  ++++      L S+L  
Sbjct: 704 LDMIRKNDIERILQERNILITVRYPFLVRFFYSFTCRDNLY--LVMEYLNGGDLYSLLQK 763

Query: 411 P--LDESSARFFAASLVMALEDLHKLGVLHRGISPDVLMLDQNGRIQLVDFRFGKRTLGE 470
              LDE  AR + A LV+ALE LH L ++HR + PD L++  NG I+L DF   K  L  
Sbjct: 764 VGCLDEEIARIYIAELVLALEYLHSLKIVHRDLKPDNLLIAYNGHIKLTDFGLSKIGLIN 823

Query: 471 RT-----------------------------YTICGMADFLAPEIVQGNGHGFAADRWAV 530
            T                             ++  G  D+LAPEI+ G  HG+AAD W+ 
Sbjct: 824 NTIDLSGHESDVSPRTNSHHFQKNQEEERIRHSAVGTPDYLAPEILLGTEHGYAADWWSA 883

Query: 531 GVLIYFMLKGEMPFGSWRRSELDTFSKIAKGQLSLPQI---FSPEAMDLIVKVMLVINNL 590
           G++++ +L G  PF + R  ++  F  I  G++  P +    S EA DLI          
Sbjct: 884 GIVLFELLTGIPPFTASRPEKI--FDNILNGKMPWPDVPGEMSYEAQDLI---------- 943

Query: 591 LYPFPFPCTLLSQSYIVIFTFCNKKYDQLLEVDEKKRLGNENQNSVRSHPWFDGVDWKGI 641
                                     ++LL  + +KRLG      V+SHP+F GVDW+ +
Sbjct: 944 --------------------------NRLLVHEPEKRLGANGAAEVKSHPFFQGVDWENL 994

BLAST of Lsi06G008270 vs. TAIR 10
Match: AT3G08720.1 (serine/threonine protein kinase 2 )

HSP 1 Score: 112.8 bits (281), Expect = 1.0e-24
Identity = 64/205 (31.22%), Postives = 102/205 (49.76%), Query Frame = 0

Query: 413 ESSARFFAASLVMALEDLHKLGVLHRGISPDVLMLDQNGRIQLVDFRFGKR-TLGERTYT 472
           E  AR + A +V A+  LH+ G++HR + P+ +++D +G + L DF   K      R+ +
Sbjct: 237 EDLARVYTAEIVSAVSHLHEKGIMHRDLKPENILMDVDGHVMLTDFGLAKEFEENTRSNS 296

Query: 473 ICGMADFLAPEIVQGNGHGFAADRWAVGVLIYFMLKGEMPF-GSWRRSELDTFSKIAKGQ 532
           +CG  +++APEIV+G GH  AAD W+VG+L+Y ML G+ PF GS  + +     KI K +
Sbjct: 297 MCGTTEYMAPEIVRGKGHDKAADWWSVGILLYEMLTGKPPFLGSKGKIQ----QKIVKDK 356

Query: 533 LSLPQIFSPEAMDLIVKVMLVINNLLYPFPFPCTLLSQSYIVIFTFCNKKYDQLLEVDEK 592
           + LPQ  S EA  L+                                      LL+ + +
Sbjct: 357 IKLPQFLSNEAHALL------------------------------------KGLLQKEPE 401

Query: 593 KRLGN--ENQNSVRSHPWFDGVDWK 614
           +RLG+       ++ H WF  ++WK
Sbjct: 417 RRLGSGPSGAEEIKKHKWFKAINWK 401

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SL764.0e-22360.69Protein phosphatase 2C and cyclic nucleotide-binding/kinase domain-containing pr... [more]
P0C6056.4e-5626.83cGMP-dependent protein kinase 1 OS=Mus musculus OX=10090 GN=Prkg1 PE=1 SV=1[more]
Q614101.1e-5526.14cGMP-dependent protein kinase 2 OS=Mus musculus OX=10090 GN=Prkg2 PE=1 SV=1[more]
Q132371.4e-5526.14cGMP-dependent protein kinase 2 OS=Homo sapiens OX=9606 GN=PRKG2 PE=1 SV=1[more]
Q645952.4e-5526.14cGMP-dependent protein kinase 2 OS=Rattus norvegicus OX=10116 GN=Prkg2 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A5D3DYE90.0e+0087.50Protein-serine/threonine phosphatase OS=Cucumis melo var. makuwa OX=1194695 GN=E... [more]
A0A1S3CHI50.0e+0087.50Protein-serine/threonine phosphatase OS=Cucumis melo OX=3656 GN=LOC103500993 PE=... [more]
A0A5A7TSX70.0e+0087.37Protein-serine/threonine phosphatase OS=Cucumis melo var. makuwa OX=1194695 GN=E... [more]
A0A1S3CJ420.0e+0087.37Protein-serine/threonine phosphatase OS=Cucumis melo OX=3656 GN=LOC103500993 PE=... [more]
A0A6J1CV030.0e+0083.99Protein-serine/threonine phosphatase OS=Momordica charantia OX=3673 GN=LOC111014... [more]
Match NameE-valueIdentityDescription
XP_038878760.10.0e+0088.55protein phosphatase 2C and cyclic nucleotide-binding/kinase domain-containing pr... [more]
XP_008462694.10.0e+0087.50PREDICTED: protein phosphatase 2C and cyclic nucleotide-binding/kinase domain-co... [more]
TYK28736.10.0e+0087.50protein phosphatase 2C and cyclic nucleotide-binding/kinase domain-containing pr... [more]
XP_038878756.10.0e+0088.42protein phosphatase 2C and cyclic nucleotide-binding/kinase domain-containing pr... [more]
KAA0046522.10.0e+0087.37protein phosphatase 2C and cyclic nucleotide-binding/kinase domain-containing pr... [more]
Match NameE-valueIdentityDescription
AT2G20050.12.8e-22460.69protein serine/threonine phosphatases;protein kinases;catalytics;cAMP-dependent ... [more]
AT2G20050.29.1e-22360.69protein serine/threonine phosphatases;protein kinases;catalytics;cAMP-dependent ... [more]
AT1G45160.14.6e-2525.83Protein kinase superfamily protein [more]
AT1G45160.24.6e-2525.83Protein kinase superfamily protein [more]
AT3G08720.11.0e-2431.22serine/threonine protein kinase 2 [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePRINTSPR00103CAMPKINASEcoord: 272..284
score: 32.05
coord: 62..76
score: 39.11
coord: 97..106
score: 60.33
coord: 47..61
score: 43.56
NoneNo IPR availableGENE3D3.30.200.20Phosphorylase Kinase; domain 1coord: 316..401
e-value: 7.0E-47
score: 161.8
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 402..549
e-value: 7.0E-47
score: 161.8
NoneNo IPR availablePIRSRPIRSR000559-2PIRSR000559-2coord: 9..548
e-value: 2.0E-82
score: 275.2
NoneNo IPR availablePANTHERPTHR24353CYCLIC NUCLEOTIDE-DEPENDENT PROTEIN KINASEcoord: 1..551
coord: 582..664
NoneNo IPR availablePANTHERPTHR24353:SF127PROTEIN PHOSPHATASE 2C AND CYCLIC NUCLEOTIDE-BINDING/KINASE DOMAIN-CONTAINING PROTEINcoord: 1..551
coord: 582..664
IPR000719Protein kinase domainSMARTSM00220serkin_6coord: 324..607
e-value: 1.1E-13
score: 61.4
IPR000719Protein kinase domainPFAMPF00069Pkinasecoord: 328..607
e-value: 6.5E-29
score: 101.1
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 317..607
score: 25.530964
IPR000595Cyclic nucleotide-binding domainSMARTSM00100cnmp_10coord: 152..291
e-value: 0.012
score: 24.8
coord: 26..149
e-value: 3.3E-11
score: 53.2
IPR000595Cyclic nucleotide-binding domainPFAMPF00027cNMP_bindingcoord: 45..134
e-value: 6.2E-18
score: 64.7
IPR000595Cyclic nucleotide-binding domainPROSITEPS50042CNMP_BINDING_3coord: 26..134
score: 26.219601
IPR000595Cyclic nucleotide-binding domainPROSITEPS50042CNMP_BINDING_3coord: 152..278
score: 16.151001
IPR000595Cyclic nucleotide-binding domainCDDcd00038CAP_EDcoord: 152..284
e-value: 1.1425E-11
score: 60.0322
IPR000595Cyclic nucleotide-binding domainCDDcd00038CAP_EDcoord: 26..134
e-value: 1.17438E-21
score: 88.5369
IPR014710RmlC-like jelly roll foldGENE3D2.60.120.10Jelly Rollscoord: 2..134
e-value: 1.7E-31
score: 110.8
IPR014710RmlC-like jelly roll foldGENE3D2.60.120.10Jelly Rollscoord: 140..299
e-value: 1.9E-21
score: 78.1
IPR018490Cyclic nucleotide-binding-likeSUPERFAMILY51206cAMP-binding domain-likecoord: 145..283
IPR018490Cyclic nucleotide-binding-likeSUPERFAMILY51206cAMP-binding domain-likecoord: 7..135
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 317..617

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi06G008270.1Lsi06G008270.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006470 protein dephosphorylation
biological_process GO:0006468 protein phosphorylation
molecular_function GO:0005524 ATP binding
molecular_function GO:0046872 metal ion binding
molecular_function GO:0004672 protein kinase activity
molecular_function GO:0004722 protein serine/threonine phosphatase activity