Lsi06G007850 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi06G007850
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	Ribonuclease H
Location	chr06: 15969647 .. 15970216 (-)
RNA-Seq Expression	Lsi06G007850
Synteny	Lsi06G007850

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGATCCTATAAAGTATATTTTCGAGAAAACATCATTGTCTGGAAGAATAGCCAAGTGGCAAGTATTGCTATCTGAGTATGATATTGTTTATGTCACTAGAAAAGCCATAAAAGGGTGCGCAATCGCCGATTGCCTAGCTGAGTTACCAGTTGAAGACTATGAACCGATGAATTTTGAGTTTCTAGATGAAGATATTATGACGATAAAAGCACTCCAGGATATAGAAACATGGACAATGTTGTTTGATGGGGCCACGAACGAGGTAGGACATGGTGTAGGAGCTATTTTGATATCTCCAAAAAGGAAGTTATATAGCCTGACGGCAAAGTTGTACTTCGATTGCACGAATAACATGGCCGAATATGAGGCATGTAGTATGGGAGTTCAAATGGCCTTCAATATGAAGATTAAAAAGTTGAAAGTTTATGGAGATTTCCTTTTAGTAGTGCATCAACTATGTGGGGAATGGGAGACAAGAGACCCTAAATTGATTCCCTATCATCAATACATCCGAGAGTTGGCTCAAAATTTCGAATCGATCACATTTGAGCATATCATGCGAAAATAA

mRNA sequence

ATGGATCCTATAAAGTATATTTTCGAGAAAACATCATTGTCTGGAAGAATAGCCAAGTGGCAAGTATTGCTATCTGAGTATGATATTGTTTATGTCACTAGAAAAGCCATAAAAGGGTGCGCAATCGCCGATTGCCTAGCTGAGTTACCAGTTGAAGACTATGAACCGATGAATTTTGAGTTTCTAGATGAAGATATTATGACGATAAAAGCACTCCAGGATATAGAAACATGGACAATGTTGTTTGATGGGGCCACGAACGAGGTAGGACATGGTGTAGGAGCTATTTTGATATCTCCAAAAAGGAAGTTATATAGCCTGACGGCAAAGTTGTACTTCGATTGCACGAATAACATGGCCGAATATGAGGCATGTAGTATGGGAGTTCAAATGGCCTTCAATATGAAGATTAAAAAGTTGAAAGTTTATGGAGATTTCCTTTTAGTAGTGCATCAACTATGTGGGGAATGGGAGACAAGAGACCCTAAATTGATTCCCTATCATCAATACATCCGAGAGTTGGCTCAAAATTTCGAATCGATCACATTTGAGCATATCATGCGAAAATAA

Coding sequence (CDS)

ATGGATCCTATAAAGTATATTTTCGAGAAAACATCATTGTCTGGAAGAATAGCCAAGTGGCAAGTATTGCTATCTGAGTATGATATTGTTTATGTCACTAGAAAAGCCATAAAAGGGTGCGCAATCGCCGATTGCCTAGCTGAGTTACCAGTTGAAGACTATGAACCGATGAATTTTGAGTTTCTAGATGAAGATATTATGACGATAAAAGCACTCCAGGATATAGAAACATGGACAATGTTGTTTGATGGGGCCACGAACGAGGTAGGACATGGTGTAGGAGCTATTTTGATATCTCCAAAAAGGAAGTTATATAGCCTGACGGCAAAGTTGTACTTCGATTGCACGAATAACATGGCCGAATATGAGGCATGTAGTATGGGAGTTCAAATGGCCTTCAATATGAAGATTAAAAAGTTGAAAGTTTATGGAGATTTCCTTTTAGTAGTGCATCAACTATGTGGGGAATGGGAGACAAGAGACCCTAAATTGATTCCCTATCATCAATACATCCGAGAGTTGGCTCAAAATTTCGAATCGATCACATTTGAGCATATCATGCGAAAATAA

Protein sequence

MDPIKYIFEKTSLSGRIAKWQVLLSEYDIVYVTRKAIKGCAIADCLAELPVEDYEPMNFEFLDEDIMTIKALQDIETWTMLFDGATNEVGHGVGAILISPKRKLYSLTAKLYFDCTNNMAEYEACSMGVQMAFNMKIKKLKVYGDFLLVVHQLCGEWETRDPKLIPYHQYIRELAQNFESITFEHIMRK

Homology

[Close]

BLAST of Lsi06G007850 vs. ExPASy Swiss-Prot
Match: Q9HSF6 (Ribonuclease HI OS=Halobacterium salinarum (strain ATCC 700922 / JCM 11081 / NRC-1) OX=64091 GN=rnhA PE=1 SV=1)

HSP 1 Score: 57.0 bits (136), Expect = 2.7e-07
Identity = 35/109 (32.11%), Postives = 51/109 (46.79%), Query Frame = 0


Query: 82  FDGAT--NEVGHGVGAILISPKRKLYSLTAKLYFDCTNNMAEYEACSMGVQMAFNMKIKK 141
           FDGA+  N     VG +L+S    + +         TNN AEY+A    ++ A +     
Sbjct: 74  FDGASRGNPGPAAVGWVLVSGDGGIVAEGGDTIGRATNNQAEYDALIAALEAAADFGFDD 133

Query: 142 LKVYGDFLLVVHQLCGEWETRDPKLIPYHQYIRELAQNFESITFEHIMR 189
           +++ GD  LV  QL G W+T DP L       REL   F+  +  H+ R
Sbjct: 134 IELRGDSQLVEKQLTGAWDTNDPDLRRKRVRARELLTGFDDWSITHVPR 182

[Close]

BLAST of Lsi06G007850 vs. ExPASy TrEMBL
Match: A0A5D3BPI8 (RNase H domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold49G00050 PE=4 SV=1)

HSP 1 Score: 299.7 bits (766), Expect = 8.8e-78
Identity = 144/187 (77.01%), Postives = 157/187 (83.96%), Query Frame = 0


Query: 1   MDPIKYIFEKTSLSGRIAKWQVLLSEYDIVYVTRKAIKGCAIADCLAELPVEDYEPMNFE 60
           MDPIKYIFEK+SL GRIAKWQVLL EYDI+YVTRKA+KG AI DCLA  PVEDYEPM  E
Sbjct: 64  MDPIKYIFEKSSLFGRIAKWQVLLLEYDIIYVTRKAMKGSAIVDCLANFPVEDYEPMKIE 123

Query: 61  FLDEDIMTIK-ALQDIETWTMLFDGATNEVGHGVGAILISPKRKLYSLTAKLYFDCTNNM 120
           F DED+M +   LQD E WTM FDGATNEVGHGVGAIL S   K Y LT KLYFDCT+NM
Sbjct: 124 FPDEDVMIVSDTLQDTEIWTMFFDGATNEVGHGVGAILTSSDGKSYPLTTKLYFDCTHNM 183

Query: 121 AEYEACSMGVQMAFNMKIKKLKVYGDFLLVVHQLCGEWETRDPKLIPYHQYIRELAQNFE 180
           AEYEACSMGVQMA++MKIKKL+VY D LL +HQL GEWET D KLIPY +YIRELAQNFE
Sbjct: 184 AEYEACSMGVQMAYDMKIKKLQVYRDSLLAIHQLNGEWETIDSKLIPYDKYIRELAQNFE 243

Query: 181 SITFEHI 187
           SITF+H+
Sbjct: 244 SITFKHV 250

[Close]

BLAST of Lsi06G007850 vs. ExPASy TrEMBL
Match: A0A6J1E2J7 (Ribonuclease H OS=Momordica charantia OX=3673 GN=LOC111025431 PE=4 SV=1)

HSP 1 Score: 279.6 bits (714), Expect = 9.4e-72
Identity = 133/192 (69.27%), Postives = 161/192 (83.85%), Query Frame = 0


Query: 1    MDPIKYIFEKTSLSGRIAKWQVLLSEYDIVYVTRKAIKGCAIADCLAELPVEDYEPMNFE 60
            MDPIKYIFEK SLSGRIA+WQVLLSEYDIVYVTRKAIKG A+AD LA+ P+ DY P+ F+
Sbjct: 1219 MDPIKYIFEKPSLSGRIARWQVLLSEYDIVYVTRKAIKGSALADYLAQQPINDYIPVKFD 1278

Query: 61   FLDEDIMTIKALQ---DIETWTMLFDGATNEVGHGVGAILISPKRKLYSLTAKLYFDCTN 120
            F DE I TI A +   D +TWTM+FDGA+NE+GHG+G ILISPK +LY LTA+L FDCT+
Sbjct: 1279 FPDEYISTITASEESLDPQTWTMMFDGASNELGHGIGGILISPKGELYPLTARLCFDCTH 1338

Query: 121  NMAEYEACSMGVQMAFNMKIKKLKVYGDFLLVVHQLCGEWETRDPKLIPYHQYIRELAQN 180
            NMAEYEACSMGVQ A +MK+KKLKV+GD +LV+HQL GEWETRD KL+PY Q I EL+Q 
Sbjct: 1339 NMAEYEACSMGVQAAVDMKVKKLKVFGDSMLVIHQLRGEWETRDVKLLPYKQLITELSQE 1398

Query: 181  FESITFEHIMRK 190
            F+ I+F+++ R+
Sbjct: 1399 FDEISFDYLPRE 1410

[Close]

BLAST of Lsi06G007850 vs. ExPASy TrEMBL
Match: A0A5A7UKV4 (Retrovirus-related Pol polyprotein from transposon 17.6 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1163G00350 PE=4 SV=1)

HSP 1 Score: 275.8 bits (704), Expect = 1.4e-70
Identity = 132/172 (76.74%), Postives = 145/172 (84.30%), Query Frame = 0


Query: 1   MDPIKYIFEKTSLSGRIAKWQVLLSEYDIVYVTRKAIKGCAIADCLAELPVEDYEPMNFE 60
           MDPIKYIFEK SLSGRIAKWQVL SEYDI+YVTR  +KG AI DCLA+LPVEDYE M FE
Sbjct: 1   MDPIKYIFEKPSLSGRIAKWQVLPSEYDIIYVTRNVVKGSAITDCLADLPVEDYELMKFE 60

Query: 61  FLDEDIMTIK-ALQDIETWTMLFDGATNEVGHGVGAILISPKRKLYSLTAKLYFDCTNNM 120
           FLDED+MT+    QD E WTM FDGATNEVGHGVGAIL  P  K Y LTAKLYFDCTNNM
Sbjct: 61  FLDEDVMTVSDTPQDTEIWTMFFDGATNEVGHGVGAILTFPDGKSYPLTAKLYFDCTNNM 120

Query: 121 AEYEACSMGVQMAFNMKIKKLKVYGDFLLVVHQLCGEWETRDPKLIPYHQYI 172
           AEYE CSMGV+MA+++KIKKL+VY D LLV+HQL  EWETRD KLIPY++YI
Sbjct: 121 AEYETCSMGVKMAYDLKIKKLQVYEDSLLVIHQLNREWETRDSKLIPYNKYI 172

[Close]

BLAST of Lsi06G007850 vs. ExPASy TrEMBL
Match: A0A5A7T0A1 (Ribonuclease H OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold86G00070 PE=4 SV=1)

HSP 1 Score: 275.8 bits (704), Expect = 1.4e-70
Identity = 129/190 (67.89%), Postives = 160/190 (84.21%), Query Frame = 0


Query: 1   MDPIKYIFEKTSLSGRIAKWQVLLSEYDIVYVTRKAIKGCAIADCLAELPVEDYEPMNFE 60
           MDPIKYIFEK SLSGRIAKWQVLLSEYDIVYVT+KAIKG A+AD LA  PV DYEPM  +
Sbjct: 234 MDPIKYIFEKPSLSGRIAKWQVLLSEYDIVYVTKKAIKGSAVADHLAAQPVADYEPMRID 293

Query: 61  FLDEDIMTI-KALQDIETWTMLFDGATNEVGHGVGAILISPKRKLYSLTAKLYFDCTNNM 120
           FLDE+I  + K  +D ETWTMLFDGA+NE+GHG+G +LIS + K++SLTAKL F+CT+N+
Sbjct: 294 FLDENIFLVEKNARDHETWTMLFDGASNELGHGIGVVLISSEGKVFSLTAKLCFECTHNI 353

Query: 121 AEYEACSMGVQMAFNMKIKKLKVYGDFLLVVHQLCGEWETRDPKLIPYHQYIRELAQNFE 180
           AEYEAC MG+Q+A++M IKKLKV GD +LV+HQ+  EWE RD KL+PY QY+ +L+QNFE
Sbjct: 354 AEYEACIMGLQVAWDMSIKKLKVLGDSMLVIHQVKEEWEIRDAKLVPYSQYVTKLSQNFE 413

Query: 181 SITFEHIMRK 190
            I+F+H+ R+
Sbjct: 414 KISFDHVPRE 423

[Close]

BLAST of Lsi06G007850 vs. ExPASy TrEMBL
Match: A0A1S4DTT9 (uncharacterized protein LOC107990505 OS=Cucumis melo OX=3656 GN=LOC107990505 PE=4 SV=1)

HSP 1 Score: 275.8 bits (704), Expect = 1.4e-70
Identity = 132/172 (76.74%), Postives = 145/172 (84.30%), Query Frame = 0


Query: 1   MDPIKYIFEKTSLSGRIAKWQVLLSEYDIVYVTRKAIKGCAIADCLAELPVEDYEPMNFE 60
           MDPIKYIFEK SLSGRIAKWQVL SEYDI+YVTR  +KG AI DCLA+LPVEDYE M FE
Sbjct: 1   MDPIKYIFEKPSLSGRIAKWQVLPSEYDIIYVTRNVVKGSAITDCLADLPVEDYELMKFE 60

Query: 61  FLDEDIMTIK-ALQDIETWTMLFDGATNEVGHGVGAILISPKRKLYSLTAKLYFDCTNNM 120
           FLDED+MT+    QD E WTM FDGATNEVGHGVGAIL  P  K Y LTAKLYFDCTNNM
Sbjct: 61  FLDEDVMTVSDTPQDTEIWTMFFDGATNEVGHGVGAILTFPDGKSYPLTAKLYFDCTNNM 120

Query: 121 AEYEACSMGVQMAFNMKIKKLKVYGDFLLVVHQLCGEWETRDPKLIPYHQYI 172
           AEYE CSMGV+MA+++KIKKL+VY D LLV+HQL  EWETRD KLIPY++YI
Sbjct: 121 AEYETCSMGVKMAYDLKIKKLQVYEDSLLVIHQLNREWETRDSKLIPYNKYI 172

[Close]

BLAST of Lsi06G007850 vs. NCBI nr
Match: KAA0033845.1 (uncharacterized protein E6C27_scaffold43059G00190 [Cucumis melo var. makuwa] >TYK01227.1 uncharacterized protein E5676_scaffold49G00050 [Cucumis melo var. makuwa])

HSP 1 Score: 299.7 bits (766), Expect = 1.8e-77
Identity = 144/187 (77.01%), Postives = 157/187 (83.96%), Query Frame = 0


Query: 1   MDPIKYIFEKTSLSGRIAKWQVLLSEYDIVYVTRKAIKGCAIADCLAELPVEDYEPMNFE 60
           MDPIKYIFEK+SL GRIAKWQVLL EYDI+YVTRKA+KG AI DCLA  PVEDYEPM  E
Sbjct: 64  MDPIKYIFEKSSLFGRIAKWQVLLLEYDIIYVTRKAMKGSAIVDCLANFPVEDYEPMKIE 123

Query: 61  FLDEDIMTIK-ALQDIETWTMLFDGATNEVGHGVGAILISPKRKLYSLTAKLYFDCTNNM 120
           F DED+M +   LQD E WTM FDGATNEVGHGVGAIL S   K Y LT KLYFDCT+NM
Sbjct: 124 FPDEDVMIVSDTLQDTEIWTMFFDGATNEVGHGVGAILTSSDGKSYPLTTKLYFDCTHNM 183

Query: 121 AEYEACSMGVQMAFNMKIKKLKVYGDFLLVVHQLCGEWETRDPKLIPYHQYIRELAQNFE 180
           AEYEACSMGVQMA++MKIKKL+VY D LL +HQL GEWET D KLIPY +YIRELAQNFE
Sbjct: 184 AEYEACSMGVQMAYDMKIKKLQVYRDSLLAIHQLNGEWETIDSKLIPYDKYIRELAQNFE 243

Query: 181 SITFEHI 187
           SITF+H+
Sbjct: 244 SITFKHV 250

[Close]

BLAST of Lsi06G007850 vs. NCBI nr
Match: XP_022158986.1 (LOW QUALITY PROTEIN: uncharacterized protein LOC111025431 [Momordica charantia])

HSP 1 Score: 279.6 bits (714), Expect = 1.9e-71
Identity = 133/192 (69.27%), Postives = 161/192 (83.85%), Query Frame = 0


Query: 1    MDPIKYIFEKTSLSGRIAKWQVLLSEYDIVYVTRKAIKGCAIADCLAELPVEDYEPMNFE 60
            MDPIKYIFEK SLSGRIA+WQVLLSEYDIVYVTRKAIKG A+AD LA+ P+ DY P+ F+
Sbjct: 1219 MDPIKYIFEKPSLSGRIARWQVLLSEYDIVYVTRKAIKGSALADYLAQQPINDYIPVKFD 1278

Query: 61   FLDEDIMTIKALQ---DIETWTMLFDGATNEVGHGVGAILISPKRKLYSLTAKLYFDCTN 120
            F DE I TI A +   D +TWTM+FDGA+NE+GHG+G ILISPK +LY LTA+L FDCT+
Sbjct: 1279 FPDEYISTITASEESLDPQTWTMMFDGASNELGHGIGGILISPKGELYPLTARLCFDCTH 1338

Query: 121  NMAEYEACSMGVQMAFNMKIKKLKVYGDFLLVVHQLCGEWETRDPKLIPYHQYIRELAQN 180
            NMAEYEACSMGVQ A +MK+KKLKV+GD +LV+HQL GEWETRD KL+PY Q I EL+Q 
Sbjct: 1339 NMAEYEACSMGVQAAVDMKVKKLKVFGDSMLVIHQLRGEWETRDVKLLPYKQLITELSQE 1398

Query: 181  FESITFEHIMRK 190
            F+ I+F+++ R+
Sbjct: 1399 FDEISFDYLPRE 1410

[Close]

BLAST of Lsi06G007850 vs. NCBI nr
Match: XP_016899404.1 (PREDICTED: uncharacterized protein LOC107990505 [Cucumis melo] >KAA0054926.1 Retrovirus-related Pol polyprotein from transposon 17.6 [Cucumis melo var. makuwa] >TYK22715.1 Retrovirus-related Pol polyprotein from transposon 17.6 [Cucumis melo var. makuwa])

HSP 1 Score: 275.8 bits (704), Expect = 2.8e-70
Identity = 132/172 (76.74%), Postives = 145/172 (84.30%), Query Frame = 0


Query: 1   MDPIKYIFEKTSLSGRIAKWQVLLSEYDIVYVTRKAIKGCAIADCLAELPVEDYEPMNFE 60
           MDPIKYIFEK SLSGRIAKWQVL SEYDI+YVTR  +KG AI DCLA+LPVEDYE M FE
Sbjct: 1   MDPIKYIFEKPSLSGRIAKWQVLPSEYDIIYVTRNVVKGSAITDCLADLPVEDYELMKFE 60

Query: 61  FLDEDIMTIK-ALQDIETWTMLFDGATNEVGHGVGAILISPKRKLYSLTAKLYFDCTNNM 120
           FLDED+MT+    QD E WTM FDGATNEVGHGVGAIL  P  K Y LTAKLYFDCTNNM
Sbjct: 61  FLDEDVMTVSDTPQDTEIWTMFFDGATNEVGHGVGAILTFPDGKSYPLTAKLYFDCTNNM 120

Query: 121 AEYEACSMGVQMAFNMKIKKLKVYGDFLLVVHQLCGEWETRDPKLIPYHQYI 172
           AEYE CSMGV+MA+++KIKKL+VY D LLV+HQL  EWETRD KLIPY++YI
Sbjct: 121 AEYETCSMGVKMAYDLKIKKLQVYEDSLLVIHQLNREWETRDSKLIPYNKYI 172

[Close]

BLAST of Lsi06G007850 vs. NCBI nr
Match: KAA0036934.1 (uncharacterized protein E6C27_scaffold86G00070 [Cucumis melo var. makuwa])

HSP 1 Score: 275.8 bits (704), Expect = 2.8e-70
Identity = 129/190 (67.89%), Postives = 160/190 (84.21%), Query Frame = 0


Query: 1   MDPIKYIFEKTSLSGRIAKWQVLLSEYDIVYVTRKAIKGCAIADCLAELPVEDYEPMNFE 60
           MDPIKYIFEK SLSGRIAKWQVLLSEYDIVYVT+KAIKG A+AD LA  PV DYEPM  +
Sbjct: 234 MDPIKYIFEKPSLSGRIAKWQVLLSEYDIVYVTKKAIKGSAVADHLAAQPVADYEPMRID 293

Query: 61  FLDEDIMTI-KALQDIETWTMLFDGATNEVGHGVGAILISPKRKLYSLTAKLYFDCTNNM 120
           FLDE+I  + K  +D ETWTMLFDGA+NE+GHG+G +LIS + K++SLTAKL F+CT+N+
Sbjct: 294 FLDENIFLVEKNARDHETWTMLFDGASNELGHGIGVVLISSEGKVFSLTAKLCFECTHNI 353

Query: 121 AEYEACSMGVQMAFNMKIKKLKVYGDFLLVVHQLCGEWETRDPKLIPYHQYIRELAQNFE 180
           AEYEAC MG+Q+A++M IKKLKV GD +LV+HQ+  EWE RD KL+PY QY+ +L+QNFE
Sbjct: 354 AEYEACIMGLQVAWDMSIKKLKVLGDSMLVIHQVKEEWEIRDAKLVPYSQYVTKLSQNFE 413

Query: 181 SITFEHIMRK 190
            I+F+H+ R+
Sbjct: 414 KISFDHVPRE 423

[Close]

BLAST of Lsi06G007850 vs. NCBI nr
Match: XP_022147189.1 (LOW QUALITY PROTEIN: uncharacterized protein LOC111016200 [Momordica charantia])

HSP 1 Score: 274.2 bits (700), Expect = 8.2e-70
Identity = 130/192 (67.71%), Postives = 160/192 (83.33%), Query Frame = 0


Query: 1    MDPIKYIFEKTSLSGRIAKWQVLLSEYDIVYVTRKAIKGCAIADCLAELPVEDYEPMNFE 60
            MDPIKYIFEK SLSG IA+WQVLLSEYDIVYVT+KAIKG A+AD LA+ P+ DY P+ F+
Sbjct: 1610 MDPIKYIFEKPSLSGGIARWQVLLSEYDIVYVTQKAIKGSALADYLAQQPINDYIPVKFD 1669

Query: 61   FLDEDIMTIKALQ---DIETWTMLFDGATNEVGHGVGAILISPKRKLYSLTAKLYFDCTN 120
            F DE I TI A +   D +TWTM+FDGA+NE+GHG+GAILISPK +LY L A+L FDC +
Sbjct: 1670 FPDEYISTITASEESLDPQTWTMMFDGASNELGHGIGAILISPKGELYPLIARLCFDCKH 1729

Query: 121  NMAEYEACSMGVQMAFNMKIKKLKVYGDFLLVVHQLCGEWETRDPKLIPYHQYIRELAQN 180
            NMAEYEACSMGVQ A +MK+KKLKV+GD +LV+HQL GEWETRD KL+PY Q+I EL+Q 
Sbjct: 1730 NMAEYEACSMGVQAAIDMKVKKLKVFGDSMLVIHQLRGEWETRDVKLLPYKQFITELSQE 1789

Query: 181  FESITFEHIMRK 190
            F+ I+F+++ R+
Sbjct: 1790 FDEISFDYLPRE 1801

[Close]

BLAST of Lsi06G007850 vs. TAIR 10
Match: AT3G01410.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 70.9 bits (172), Expect = 1.3e-12
Identity = 38/130 (29.23%), Postives = 69/130 (53.08%), Query Frame = 0


Query: 62  LDEDIMTIKALQDIETWTMLFDGAT--NEVGHGVGAILISPKRKLYSLTAKLYFDCTNNM 121
           +   ++T   ++  ++ T+ FDGA+  N    G GA+L +    +     +   + TNN+
Sbjct: 139 IPSSLLTRTPIRQNDSCTIEFDGASKGNPGKAGAGAVLRASDNSVLFYLREGVGNATNNV 198

Query: 122 AEYEACSMGVQMAFNMKIKKLKVYGDFLLVVHQLCGEWETRDPKLIPYHQYIRELAQNFE 181
           AEY A  +G++ A +   K + V GD +LV  Q+ G W+T  PK+    +  +EL  +F+
Sbjct: 199 AEYRALLLGLRSALDKGFKNVHVLGDSMLVCMQVQGAWKTNHPKMAELCKQAKELMNSFK 258

Query: 182 SITFEHIMRK 190
           +   +HI R+
Sbjct: 259 TFDIKHIARE 268

[Close]

BLAST of Lsi06G007850 vs. TAIR 10
Match: AT3G01410.2 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 70.9 bits (172), Expect = 1.3e-12
Identity = 38/130 (29.23%), Postives = 69/130 (53.08%), Query Frame = 0


Query: 62  LDEDIMTIKALQDIETWTMLFDGAT--NEVGHGVGAILISPKRKLYSLTAKLYFDCTNNM 121
           +   ++T   ++  ++ T+ FDGA+  N    G GA+L +    +     +   + TNN+
Sbjct: 139 IPSSLLTRTPIRQNDSCTIEFDGASKGNPGKAGAGAVLRASDNSVLFYLREGVGNATNNV 198

Query: 122 AEYEACSMGVQMAFNMKIKKLKVYGDFLLVVHQLCGEWETRDPKLIPYHQYIRELAQNFE 181
           AEY A  +G++ A +   K + V GD +LV  Q+ G W+T  PK+    +  +EL  +F+
Sbjct: 199 AEYRALLLGLRSALDKGFKNVHVLGDSMLVCMQVQGAWKTNHPKMAELCKQAKELMNSFK 258

Query: 182 SITFEHIMRK 190
           +   +HI R+
Sbjct: 259 TFDIKHIARE 268

The following BLAST results are available for this feature:

BLAST of Lsi06G007850 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9HSF6	2.7e-07	32.11	Ribonuclease HI OS=Halobacterium salinarum (strain ATCC 700922 / JCM 11081 / NRC...	[more]

BLAST of Lsi06G007850 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5D3BPI8	8.8e-78	77.01	RNase H domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E567...	[more]
A0A6J1E2J7	9.4e-72	69.27	Ribonuclease H OS=Momordica charantia OX=3673 GN=LOC111025431 PE=4 SV=1	[more]
A0A5A7UKV4	1.4e-70	76.74	Retrovirus-related Pol polyprotein from transposon 17.6 OS=Cucumis melo var. mak...	[more]
A0A5A7T0A1	1.4e-70	67.89	Ribonuclease H OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold86G00070 ...	[more]
A0A1S4DTT9	1.4e-70	76.74	uncharacterized protein LOC107990505 OS=Cucumis melo OX=3656 GN=LOC107990505 PE=...	[more]

BLAST of Lsi06G007850 vs. NCBI nr


Match Name	E-value	Identity	Description
KAA0033845.1	1.8e-77	77.01	uncharacterized protein E6C27_scaffold43059G00190 [Cucumis melo var. makuwa] >TY...	[more]
XP_022158986.1	1.9e-71	69.27	LOW QUALITY PROTEIN: uncharacterized protein LOC111025431 [Momordica charantia]	[more]
XP_016899404.1	2.8e-70	76.74	PREDICTED: uncharacterized protein LOC107990505 [Cucumis melo] >KAA0054926.1 Ret...	[more]
KAA0036934.1	2.8e-70	67.89	uncharacterized protein E6C27_scaffold86G00070 [Cucumis melo var. makuwa]	[more]
XP_022147189.1	8.2e-70	67.71	LOW QUALITY PROTEIN: uncharacterized protein LOC111016200 [Momordica charantia]	[more]

BLAST of Lsi06G007850 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G01410.1	1.3e-12	29.23	Polynucleotidyl transferase, ribonuclease H-like superfamily protein	[more]
AT3G01410.2	1.3e-12	29.23	Polynucleotidyl transferase, ribonuclease H-like superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR036397	Ribonuclease H superfamily	GENE3D	3.30.420.10		coord: 79..189 e-value: 2.5E-24 score: 87.7
IPR002156	Ribonuclease H domain	PFAM	PF13456	RVT_3	coord: 82..188 e-value: 1.3E-16 score: 60.6
IPR002156	Ribonuclease H domain	PROSITE	PS50879	RNASE_H	coord: 74..189 score: 11.191744
None	No IPR available	PANTHER	PTHR24559:SF322	RNA-DIRECTED DNA POLYMERASE (REVERSE TRANSCRIPTASE), RIBONUCLEASE H-LIKE PROTEIN	coord: 2..188
None	No IPR available	PANTHER	PTHR24559	TRANSPOSON TY3-I GAG-POL POLYPROTEIN	coord: 2..188
None	No IPR available	CDD	cd09279	RNase_HI_like	coord: 78..189 e-value: 6.03541E-38 score: 125.278
IPR012337	Ribonuclease H-like superfamily	SUPERFAMILY	53098	Ribonuclease H-like	coord: 76..188

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi06G007850.1	Lsi06G007850.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0071897	DNA biosynthetic process
biological_process	GO:0015074	DNA integration
biological_process	GO:0006310	DNA recombination
biological_process	GO:0090502	RNA phosphodiester bond hydrolysis, endonucleolytic
cellular_component	GO:0030430	host cell cytoplasm
cellular_component	GO:0005634	nucleus
molecular_function	GO:0003887	DNA-directed DNA polymerase activity
molecular_function	GO:0003723	RNA binding
molecular_function	GO:0004523	RNA-DNA hybrid ribonuclease activity
molecular_function	GO:0003676	nucleic acid binding