Lsi06G006960 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi06G006960
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	seipin-1
Location	chr06: 11056237 .. 11056980 (+)
RNA-Seq Expression	Lsi06G006960
Synteny	Lsi06G006960

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAATTATCTAAACTCAATATTTGCTGATCATCAGTTGAGTGCAGAGTTGATATCAACAAATGGAAACATAATAACCGGTTCAAGCCAACCCTGCATGCTCCGGTTCAGAAGCACACCGGTCCGGTTGACCCGAACTTTTCTCACTAGCTTTCCTCTCCTCTTAGGAATCTCAAGTGAATCCCAAAGGCTAACTTTTCCCATACTAGAGCACAAGGAAGAAAATCACCAACGAAGTGCAGCCATCCATGTTACTATTAGCCCTCGCATTGGAACCACGGCCCTCCCCGAGCTCTACGAAGCTCATATTTTAATAAACTCGAAACTACCAAAGACGAAAGAGTTGTTGCATCGGTGGCGATGGACTTGCTTCCTATGGATGTCTCTTTATTTGTACGTGATGTTTCTAGTGATCTTTATGTTCTTTTGGAAGCCGGTTGTGTTTCGTGCAATGACGTTGTCGGAGTTGAGTGGTTTTGATGAAGGAGCTCCGACGACGAAGGAAGCAGAGGAGTCGTTCGATGAGATGGCGGAGATAACGGTGGAGTTGTTGAGAAAATGGCAAGAGATAAGGAGGAAGAGAAAGGCTGCCGTGTTTGGGTATGGAGAAGTTGGAGGAGGAGAAGATGACGTTGGGTCGACATCTGCGTCGAGCATTAGTTGTAGCAGAGAGTATGTAGGTGCTGTTTTCGAGGAAGATGTCGAGGACTCGGAGTCGGTGCTTCTAGAAGGTTCGGAGGAGTAG

mRNA sequence

ATGAATTATCTAAACTCAATATTTGCTGATCATCAGTTGAGTGCAGAGTTGATATCAACAAATGGAAACATAATAACCGGTTCAAGCCAACCCTGCATGCTCCGGTTCAGAAGCACACCGGTCCGGTTGACCCGAACTTTTCTCACTAGCTTTCCTCTCCTCTTAGGAATCTCAAGTGAATCCCAAAGGCTAACTTTTCCCATACTAGAGCACAAGGAAGAAAATCACCAACGAAGTGCAGCCATCCATGTTACTATTAGCCCTCGCATTGGAACCACGGCCCTCCCCGAGCTCTACGAAGCTCATATTTTAATAAACTCGAAACTACCAAAGACGAAAGAGTTGTTGCATCGGTGGCGATGGACTTGCTTCCTATGGATGTCTCTTTATTTGTACGTGATGTTTCTAGTGATCTTTATGTTCTTTTGGAAGCCGGTTGTGTTTCGTGCAATGACGTTGTCGGAGTTGAGTGGTTTTGATGAAGGAGCTCCGACGACGAAGGAAGCAGAGGAGTCGTTCGATGAGATGGCGGAGATAACGGTGGAGTTGTTGAGAAAATGGCAAGAGATAAGGAGGAAGAGAAAGGCTGCCGTGTTTGGGTATGGAGAAGTTGGAGGAGGAGAAGATGACGTTGGGTCGACATCTGCGTCGAGCATTAGTTGTAGCAGAGAGTATGTAGGTGCTGTTTTCGAGGAAGATGTCGAGGACTCGGAGTCGGTGCTTCTAGAAGGTTCGGAGGAGTAG

Coding sequence (CDS)

ATGAATTATCTAAACTCAATATTTGCTGATCATCAGTTGAGTGCAGAGTTGATATCAACAAATGGAAACATAATAACCGGTTCAAGCCAACCCTGCATGCTCCGGTTCAGAAGCACACCGGTCCGGTTGACCCGAACTTTTCTCACTAGCTTTCCTCTCCTCTTAGGAATCTCAAGTGAATCCCAAAGGCTAACTTTTCCCATACTAGAGCACAAGGAAGAAAATCACCAACGAAGTGCAGCCATCCATGTTACTATTAGCCCTCGCATTGGAACCACGGCCCTCCCCGAGCTCTACGAAGCTCATATTTTAATAAACTCGAAACTACCAAAGACGAAAGAGTTGTTGCATCGGTGGCGATGGACTTGCTTCCTATGGATGTCTCTTTATTTGTACGTGATGTTTCTAGTGATCTTTATGTTCTTTTGGAAGCCGGTTGTGTTTCGTGCAATGACGTTGTCGGAGTTGAGTGGTTTTGATGAAGGAGCTCCGACGACGAAGGAAGCAGAGGAGTCGTTCGATGAGATGGCGGAGATAACGGTGGAGTTGTTGAGAAAATGGCAAGAGATAAGGAGGAAGAGAAAGGCTGCCGTGTTTGGGTATGGAGAAGTTGGAGGAGGAGAAGATGACGTTGGGTCGACATCTGCGTCGAGCATTAGTTGTAGCAGAGAGTATGTAGGTGCTGTTTTCGAGGAAGATGTCGAGGACTCGGAGTCGGTGCTTCTAGAAGGTTCGGAGGAGTAG

Protein sequence

MNYLNSIFADHQLSAELISTNGNIITGSSQPCMLRFRSTPVRLTRTFLTSFPLLLGISSESQRLTFPILEHKEENHQRSAAIHVTISPRIGTTALPELYEAHILINSKLPKTKELLHRWRWTCFLWMSLYLYVMFLVIFMFFWKPVVFRAMTLSELSGFDEGAPTTKEAEESFDEMAEITVELLRKWQEIRRKRKAAVFGYGEVGGGEDDVGSTSASSISCSREYVGAVFEEDVEDSESVLLEGSEE

Homology

[Close]

BLAST of Lsi06G006960 vs. ExPASy Swiss-Prot
Match: Q9FFD9 (Seipin-1 OS=Arabidopsis thaliana OX=3702 GN=SEI1 PE=2 SV=1)

HSP 1 Score: 128.6 bits (322), Expect = 9.5e-29
Identity = 76/184 (41.30%), Postives = 105/184 (57.07%), Query Frame = 0


Query: 12  QLSAELISTNGNIITGSSQPCMLRFRSTPVRLTRTFLTSFPLLLGISSESQRLTFPILEH 71
           QL  EL+S  G  I  SSQPCMLRFRS P+RL RTF+ S PL+ GI++E+Q +    L+H
Sbjct: 182 QLKVELLSLKGETIARSSQPCMLRFRSKPIRLARTFVMSVPLIAGIANEAQTMRIDALKH 241

Query: 72  KEENHQRSAAIHVTISPRIGTTALPELYEAHILINSKLPKTKELLHRWRWTCFLWMSLYL 131
            +E   R+ A+  T+ PR  T  LP+LYEA I+INSK P  K + + W+WT  +W S+YL
Sbjct: 242 -QEKMPRTKAVRATLIPRAQTRTLPQLYEAEIVINSKPPWIKRMAYNWKWTLCVWTSMYL 301

Query: 132 YVMFLVIFMFFWKPVVFRAMTLSELSGFDEGAPTTKEAEESFDEMAEITVELLRKWQEIR 191
           YV  L   ++ ++PV+F               P T     S  E  EI V    + Q + 
Sbjct: 302 YVAILTALLWCFRPVLF---------------PYTSSRTISESENLEIEVVEEEQEQVME 349

Query: 192 RKRK 196
           R+R+
Sbjct: 362 RRRR 349

[Close]

BLAST of Lsi06G006960 vs. ExPASy Swiss-Prot
Match: Q8L615 (Seipin-3 OS=Arabidopsis thaliana OX=3702 GN=SEI3 PE=2 SV=1)

HSP 1 Score: 86.3 bits (212), Expect = 5.4e-16
Identity = 45/139 (32.37%), Postives = 82/139 (58.99%), Query Frame = 0


Query: 12  QLSAELISTNGNIITGSSQPCMLRFRSTPVRLTRTFLTSFPLLLGISSESQRLTFPILEH 71
           Q+  + +S +G+++  S +PCM++F S P+RL +T L   PL+ G  SE Q L   +   
Sbjct: 341 QVRVDFLSASGHVLASSRRPCMVKFSSEPIRLVQTLLKIAPLVTGYVSEIQTLNLKLKGL 400

Query: 72  KEENHQRSAAIHVTISPRI---GTTALPELYEAHILINSKLPKTKELLHRWRWTCFLWMS 131
            E++   +A + + I  R        +PE+Y+A + + SKLP  K ++  WR T F+W+S
Sbjct: 401 VEKDIIPTACLKIMIEQRAEFRPGAGIPEIYDASLFLESKLPFLKRIIWNWRKTLFVWIS 460

Query: 132 LYLYVMFLVIFMFFWKPVV 148
           + L++M L+  + F++P++
Sbjct: 461 MSLFIMELLFALVFFRPLI 479

[Close]

BLAST of Lsi06G006960 vs. ExPASy Swiss-Prot
Match: F4I340 (Seipin-2 OS=Arabidopsis thaliana OX=3702 GN=SEI2 PE=2 SV=1)

HSP 1 Score: 77.4 bits (189), Expect = 2.5e-13
Identity = 45/139 (32.37%), Postives = 79/139 (56.83%), Query Frame = 0


Query: 12  QLSAELISTNGNIITGSSQPCMLRFRSTPVRLTRTFLTSFPLLLGISSESQRLTFPILEH 71
           Q+  + +S +G  I    +PCMLRFRS P+RL +TF    PL+ G  SE Q L+  +   
Sbjct: 370 QVRVDFLSVDGQTIASIRRPCMLRFRSEPIRLVQTFFKVVPLVTGYVSEIQTLSLKLKGF 429

Query: 72  KEENHQRSAAIHVTISPRI---GTTALPELYEAHILINSKLPKTKELLHRWRWTCFLWMS 131
            E++   +A + + I  R        +PELY+A + + S LP  ++++ +WR T F+W+S
Sbjct: 430 VEKDIP-TACLKIIIEQRAEFRPGAGIPELYDASLSVESGLPFFRKIIWKWRKTLFVWIS 489

Query: 132 LYLYVMFLVIFMFFWKPVV 148
           + L++  L+  +   +P++
Sbjct: 490 MSLFITELLFTLVCCRPLI 507

[Close]

BLAST of Lsi06G006960 vs. ExPASy TrEMBL
Match: A0A1S3B8L6 (seipin-1 OS=Cucumis melo OX=3656 GN=LOC103487228 PE=4 SV=1)

HSP 1 Score: 356.7 bits (914), Expect = 7.9e-95
Identity = 191/247 (77.33%), Postives = 213/247 (86.23%), Query Frame = 0


Query: 5   NSIFADHQLSAELISTNGNIITGSSQPCMLRFRSTPVRLTRTFLTSFPLLLGISSESQRL 64
           N  F   QLSAELISTNGNIIT SSQPCMLRFRS PVR  RT LTSFP+L+GIS+E+QRL
Sbjct: 183 NREFGVFQLSAELISTNGNIITSSSQPCMLRFRSAPVRFARTLLTSFPILIGISTEAQRL 242

Query: 65  TFPILEHKEENHQRSAAIHVTISPRIGTTALPELYEAHILINSKLPKTKELLHRWRWTCF 124
           +FPIL+HKEEN +RSAAI  TISPRIGT+ALPELYEAHI+INSKLP+ KELL RWRWTCF
Sbjct: 243 SFPILKHKEENQERSAAIQFTISPRIGTSALPELYEAHIVINSKLPRMKELLRRWRWTCF 302

Query: 125 LWMSLYLYVMFLVIFMFFWKPVVFRAMTLS-ELSGFDEGAPTTKE---AEESFDEMAEIT 184
           LW S+YLY+MFL IFM FWKPV+FRAMTL+ ELS FD+  P  +E   A+ES DEMAEIT
Sbjct: 303 LWTSIYLYLMFLAIFMLFWKPVMFRAMTLTPELSDFDQD-PRRRELEGADESLDEMAEIT 362

Query: 185 VELLRKWQEIRRKRKAAVFGYGEVGGGEDDVGSTSASSISCSREYVGAVFEEDVEDSESV 244
           VELLRKWQE+RRKRKAA+FGY   G GE+DVGSTSASSISCSR+Y+ AVFEEDV DSESV
Sbjct: 363 VELLRKWQEMRRKRKAAMFGY---GSGEEDVGSTSASSISCSRDYIAAVFEEDVGDSESV 422

Query: 245 LLEGSEE 248
           LLEGSEE
Sbjct: 423 LLEGSEE 425

[Close]

BLAST of Lsi06G006960 vs. ExPASy TrEMBL
Match: A0A5D3E501 (Seipin-1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold84664G00880 PE=4 SV=1)

HSP 1 Score: 353.2 bits (905), Expect = 8.7e-94
Identity = 188/239 (78.66%), Postives = 210/239 (87.87%), Query Frame = 0


Query: 13  LSAELISTNGNIITGSSQPCMLRFRSTPVRLTRTFLTSFPLLLGISSESQRLTFPILEHK 72
           LSAELISTNGNIIT SSQPCMLRFRS PVR  RT LTSFP+L+GIS+E+QRL+FPIL+HK
Sbjct: 163 LSAELISTNGNIITSSSQPCMLRFRSAPVRFARTLLTSFPILIGISTEAQRLSFPILKHK 222

Query: 73  EENHQRSAAIHVTISPRIGTTALPELYEAHILINSKLPKTKELLHRWRWTCFLWMSLYLY 132
           EEN +RSAAI  TISPRIGT+ALPELYEAHI+INSKLP+ KELL RWRWTCFLW S+YLY
Sbjct: 223 EENQERSAAIQFTISPRIGTSALPELYEAHIVINSKLPRMKELLRRWRWTCFLWTSIYLY 282

Query: 133 VMFLVIFMFFWKPVVFRAMTLS-ELSGFDEGAPTTKE---AEESFDEMAEITVELLRKWQ 192
           +MFL IFM FWKPV+FRAMTL+ ELS FD+  P  +E   A+ES DEMAEITVELLRKWQ
Sbjct: 283 LMFLAIFMLFWKPVMFRAMTLTPELSDFDQD-PRRRELEGADESLDEMAEITVELLRKWQ 342

Query: 193 EIRRKRKAAVFGYGEVGGGEDDVGSTSASSISCSREYVGAVFEEDVEDSESVLLEGSEE 248
           E+RRKRKAA+FGY   G GE+DVGSTSASSISCSR+Y+ AVFEEDV DSESVLLEGSEE
Sbjct: 343 EMRRKRKAAMFGY---GSGEEDVGSTSASSISCSRDYIAAVFEEDVGDSESVLLEGSEE 397

[Close]

BLAST of Lsi06G006960 vs. ExPASy TrEMBL
Match: A0A5A7T443 (Seipin-1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold270G001120 PE=4 SV=1)

HSP 1 Score: 351.7 bits (901), Expect = 2.5e-93
Identity = 187/239 (78.24%), Postives = 210/239 (87.87%), Query Frame = 0


Query: 13  LSAELISTNGNIITGSSQPCMLRFRSTPVRLTRTFLTSFPLLLGISSESQRLTFPILEHK 72
           LSAELISTNGNIIT SSQPCMLRFRS PVR  RT LTSFP+L+GIS+E+QRL+FPIL+HK
Sbjct: 163 LSAELISTNGNIITSSSQPCMLRFRSAPVRFARTLLTSFPILIGISTEAQRLSFPILKHK 222

Query: 73  EENHQRSAAIHVTISPRIGTTALPELYEAHILINSKLPKTKELLHRWRWTCFLWMSLYLY 132
           EEN +RSAAI  TISPRIGT+ALPELYEAHI+INSKLP+ KELL RWRWTCFLW S+YLY
Sbjct: 223 EENQERSAAIQFTISPRIGTSALPELYEAHIVINSKLPRMKELLRRWRWTCFLWTSIYLY 282

Query: 133 VMFLVIFMFFWKPVVFRAMTLS-ELSGFDEGAPTTKE---AEESFDEMAEITVELLRKWQ 192
           +MFL IFM FWKPV+FRAMTL+ ELS FD+  P  +E   A+ES +EMAEITVELLRKWQ
Sbjct: 283 LMFLAIFMLFWKPVMFRAMTLTPELSDFDQD-PRRRELEGADESLEEMAEITVELLRKWQ 342

Query: 193 EIRRKRKAAVFGYGEVGGGEDDVGSTSASSISCSREYVGAVFEEDVEDSESVLLEGSEE 248
           E+RRKRKAA+FGY   G GE+DVGSTSASSISCSR+Y+ AVFEEDV DSESVLLEGSEE
Sbjct: 343 EMRRKRKAAMFGY---GSGEEDVGSTSASSISCSRDYIAAVFEEDVGDSESVLLEGSEE 397

[Close]

BLAST of Lsi06G006960 vs. ExPASy TrEMBL
Match: A0A6J1KZV1 (seipin-1 OS=Cucurbita maxima OX=3661 GN=LOC111498585 PE=4 SV=1)

HSP 1 Score: 349.7 bits (896), Expect = 9.7e-93
Identity = 176/236 (74.58%), Postives = 208/236 (88.14%), Query Frame = 0


Query: 12  QLSAELISTNGNIITGSSQPCMLRFRSTPVRLTRTFLTSFPLLLGISSESQRLTFPILEH 71
           QLSAELISTNGNII GSSQPCMLRFRSTPVR TRTFL+  PLLLG+S+ESQ+LTFP+L+H
Sbjct: 254 QLSAELISTNGNIIAGSSQPCMLRFRSTPVRFTRTFLSGLPLLLGLSTESQKLTFPVLKH 313

Query: 72  KEENHQRSAAIHVTISPRIGTTALPELYEAHILINSKLPKTKELLHRWRWTCFLWMSLYL 131
           KE+++QRS AI VTI+PRIGT+ALPELYEAHILINSKLP TKEL+HRWRWTCFLW S Y 
Sbjct: 314 KEQSYQRSGAIQVTITPRIGTSALPELYEAHILINSKLPWTKELVHRWRWTCFLWTSFYF 373

Query: 132 YVMFLVIFMFFWKPVVFRAMTLSELSGFDEGAPTTKEAEESFDEMAEITVELLRKWQEIR 191
           Y+ FL IF++FW+P+VFRA+T++   GFD  A  +KE E+SFDEMAE+TVELLRKWQE+R
Sbjct: 374 YLTFLAIFVYFWRPIVFRAVTVARF-GFDREASMSKEVEKSFDEMAEVTVELLRKWQEMR 433

Query: 192 RKRKAAVFGYGEVGGGEDDVGSTSASSISCSREYVGAVFEEDVEDSESVLLEGSEE 248
           RKRKAA+FGY      ++DVGSTSASSISCSREY+G++FEEDV DSES++LEG EE
Sbjct: 434 RKRKAALFGY------DEDVGSTSASSISCSREYIGSIFEEDVGDSESMVLEGWEE 482

[Close]

BLAST of Lsi06G006960 vs. ExPASy TrEMBL
Match: A0A6J1F5S3 (seipin-1 OS=Cucurbita moschata OX=3662 GN=LOC111441088 PE=4 SV=1)

HSP 1 Score: 346.7 bits (888), Expect = 8.2e-92
Identity = 175/236 (74.15%), Postives = 209/236 (88.56%), Query Frame = 0


Query: 12  QLSAELISTNGNIITGSSQPCMLRFRSTPVRLTRTFLTSFPLLLGISSESQRLTFPILEH 71
           QLSAELISTNGNII GSSQPCMLRFRSTPVR TRTFL+S PLLLG+S+ESQ+LTFP+L++
Sbjct: 239 QLSAELISTNGNIIAGSSQPCMLRFRSTPVRFTRTFLSSLPLLLGLSTESQKLTFPVLKY 298

Query: 72  KEENHQRSAAIHVTISPRIGTTALPELYEAHILINSKLPKTKELLHRWRWTCFLWMSLYL 131
           KE+++QRS AI VTI+PR+GT+ALPELYEAHILINSKLP TKEL+HRWRWTCFLW S YL
Sbjct: 299 KEQSYQRSGAIQVTITPRVGTSALPELYEAHILINSKLPWTKELVHRWRWTCFLWSSFYL 358

Query: 132 YVMFLVIFMFFWKPVVFRAMTLSELSGFDEGAPTTKEAEESFDEMAEITVELLRKWQEIR 191
           Y+ FL IF++FW+P+VFRA+TL+    F+  A  +KE E+SFDEMAE+TVELLRKWQE+R
Sbjct: 359 YLTFLAIFVYFWRPIVFRAVTLARFD-FNREASISKEVEKSFDEMAEVTVELLRKWQEMR 418

Query: 192 RKRKAAVFGYGEVGGGEDDVGSTSASSISCSREYVGAVFEEDVEDSESVLLEGSEE 248
           RKRKAA+FGY      ++DVGSTSASSISCSREY+G++FEEDV DSES++LEG EE
Sbjct: 419 RKRKAALFGY------DEDVGSTSASSISCSREYIGSIFEEDVGDSESMVLEGWEE 467

[Close]

BLAST of Lsi06G006960 vs. NCBI nr
Match: XP_038901874.1 (seipin-1 isoform X2 [Benincasa hispida])

HSP 1 Score: 373.2 bits (957), Expect = 1.7e-99
Identity = 204/242 (84.30%), Postives = 217/242 (89.67%), Query Frame = 0


Query: 12  QLSAELISTNGNIITGSSQPCMLRFRSTPVRLTRTFLTSFPLLLGISSESQRLTFPILEH 71
           QLSAELISTNGNII  SSQPCMLRFRSTPVR  +TFLTS PLLLGIS+E+Q+LTFPIL H
Sbjct: 187 QLSAELISTNGNIIARSSQPCMLRFRSTPVRFAQTFLTSLPLLLGISTETQKLTFPILNH 246

Query: 72  KEENHQRSAAIHVTISPRIGTTALPELYEAHILINSKLPKTKELLHRWRWTCFLWMSLYL 131
           KEENHQRSAAI VTISPR+GT+ALPELYEAHILINSKLPK KELL RWRWTCFLW SLYL
Sbjct: 247 KEENHQRSAAIQVTISPRMGTSALPELYEAHILINSKLPKMKELLRRWRWTCFLWTSLYL 306

Query: 132 YVMFLVIFMFFWKPVVFRAMTLSELSGFDEGAPTTKEAEESFDEMAEITVELLRKWQEIR 191
           Y++FLVIFMFF KPVVFRAMTL+ELSGFD+GAPT KEAEESFDEMAEITVELLRKWQE+R
Sbjct: 307 YLLFLVIFMFFCKPVVFRAMTLAELSGFDKGAPTRKEAEESFDEMAEITVELLRKWQEMR 366

Query: 192 RKRKAAVFGYGEVGGGEDDVGS--TSASSISC-SREYVGAVFEEDVED----SESVLLEG 247
           RKRKAA+F Y EVG G  DVGS  TSASSISC SREY+ AVFEEDVED    SESVLLEG
Sbjct: 367 RKRKAAMFRYEEVGRG-GDVGSIQTSASSISCSSREYIAAVFEEDVEDSDSESESVLLEG 426

[Close]

BLAST of Lsi06G006960 vs. NCBI nr
Match: XP_038901873.1 (seipin-1 isoform X1 [Benincasa hispida])

HSP 1 Score: 371.3 bits (952), Expect = 6.4e-99
Identity = 203/242 (83.88%), Postives = 216/242 (89.26%), Query Frame = 0


Query: 12  QLSAELISTNGNIITGSSQPCMLRFRSTPVRLTRTFLTSFPLLLGISSESQRLTFPILEH 71
           QLSAELISTNGNII  SSQPCMLRFRSTPVR  +TFLTS PLLLGIS+E+Q+LTFPIL H
Sbjct: 187 QLSAELISTNGNIIARSSQPCMLRFRSTPVRFAQTFLTSLPLLLGISTETQKLTFPILNH 246

Query: 72  KEENHQRSAAIHVTISPRIGTTALPELYEAHILINSKLPKTKELLHRWRWTCFLWMSLYL 131
           KEENHQRSAAI VTISPR+GT+ALPELYEAHILINSKLPK KELL RWRWTCFLW SLYL
Sbjct: 247 KEENHQRSAAIQVTISPRMGTSALPELYEAHILINSKLPKMKELLRRWRWTCFLWTSLYL 306

Query: 132 YVMFLVIFMFFWKPVVFRAMTLSELSGFDEGAPTTKEAEESFDEMAEITVELLRKWQEIR 191
           Y++FLVIFMFF KPVVFRAMTL+ELSGFD+GAPT KEAEESFDEMAEITVELLRKWQE+R
Sbjct: 307 YLLFLVIFMFFCKPVVFRAMTLAELSGFDKGAPTRKEAEESFDEMAEITVELLRKWQEMR 366

Query: 192 RKRKAAVFGYGEVGGGEDDVGS--TSASSISC-SREYVGAVFEEDVED----SESVLLEG 247
           RKRKAA+F Y EVG G  DVGS  TSASSISC SREY+ AVFEEDVED    SESVLLEG
Sbjct: 367 RKRKAAMFRYEEVGRG-GDVGSIQTSASSISCSSREYIAAVFEEDVEDSDSESESVLLEG 426

[Close]

BLAST of Lsi06G006960 vs. NCBI nr
Match: XP_008443693.1 (PREDICTED: seipin-1 [Cucumis melo])

HSP 1 Score: 356.7 bits (914), Expect = 1.6e-94
Identity = 191/247 (77.33%), Postives = 213/247 (86.23%), Query Frame = 0


Query: 5   NSIFADHQLSAELISTNGNIITGSSQPCMLRFRSTPVRLTRTFLTSFPLLLGISSESQRL 64
           N  F   QLSAELISTNGNIIT SSQPCMLRFRS PVR  RT LTSFP+L+GIS+E+QRL
Sbjct: 183 NREFGVFQLSAELISTNGNIITSSSQPCMLRFRSAPVRFARTLLTSFPILIGISTEAQRL 242

Query: 65  TFPILEHKEENHQRSAAIHVTISPRIGTTALPELYEAHILINSKLPKTKELLHRWRWTCF 124
           +FPIL+HKEEN +RSAAI  TISPRIGT+ALPELYEAHI+INSKLP+ KELL RWRWTCF
Sbjct: 243 SFPILKHKEENQERSAAIQFTISPRIGTSALPELYEAHIVINSKLPRMKELLRRWRWTCF 302

Query: 125 LWMSLYLYVMFLVIFMFFWKPVVFRAMTLS-ELSGFDEGAPTTKE---AEESFDEMAEIT 184
           LW S+YLY+MFL IFM FWKPV+FRAMTL+ ELS FD+  P  +E   A+ES DEMAEIT
Sbjct: 303 LWTSIYLYLMFLAIFMLFWKPVMFRAMTLTPELSDFDQD-PRRRELEGADESLDEMAEIT 362

Query: 185 VELLRKWQEIRRKRKAAVFGYGEVGGGEDDVGSTSASSISCSREYVGAVFEEDVEDSESV 244
           VELLRKWQE+RRKRKAA+FGY   G GE+DVGSTSASSISCSR+Y+ AVFEEDV DSESV
Sbjct: 363 VELLRKWQEMRRKRKAAMFGY---GSGEEDVGSTSASSISCSRDYIAAVFEEDVGDSESV 422

Query: 245 LLEGSEE 248
           LLEGSEE
Sbjct: 423 LLEGSEE 425

[Close]

BLAST of Lsi06G006960 vs. NCBI nr
Match: TYK30661.1 (seipin-1 [Cucumis melo var. makuwa])

HSP 1 Score: 353.2 bits (905), Expect = 1.8e-93
Identity = 188/239 (78.66%), Postives = 210/239 (87.87%), Query Frame = 0


Query: 13  LSAELISTNGNIITGSSQPCMLRFRSTPVRLTRTFLTSFPLLLGISSESQRLTFPILEHK 72
           LSAELISTNGNIIT SSQPCMLRFRS PVR  RT LTSFP+L+GIS+E+QRL+FPIL+HK
Sbjct: 163 LSAELISTNGNIITSSSQPCMLRFRSAPVRFARTLLTSFPILIGISTEAQRLSFPILKHK 222

Query: 73  EENHQRSAAIHVTISPRIGTTALPELYEAHILINSKLPKTKELLHRWRWTCFLWMSLYLY 132
           EEN +RSAAI  TISPRIGT+ALPELYEAHI+INSKLP+ KELL RWRWTCFLW S+YLY
Sbjct: 223 EENQERSAAIQFTISPRIGTSALPELYEAHIVINSKLPRMKELLRRWRWTCFLWTSIYLY 282

Query: 133 VMFLVIFMFFWKPVVFRAMTLS-ELSGFDEGAPTTKE---AEESFDEMAEITVELLRKWQ 192
           +MFL IFM FWKPV+FRAMTL+ ELS FD+  P  +E   A+ES DEMAEITVELLRKWQ
Sbjct: 283 LMFLAIFMLFWKPVMFRAMTLTPELSDFDQD-PRRRELEGADESLDEMAEITVELLRKWQ 342

Query: 193 EIRRKRKAAVFGYGEVGGGEDDVGSTSASSISCSREYVGAVFEEDVEDSESVLLEGSEE 248
           E+RRKRKAA+FGY   G GE+DVGSTSASSISCSR+Y+ AVFEEDV DSESVLLEGSEE
Sbjct: 343 EMRRKRKAAMFGY---GSGEEDVGSTSASSISCSRDYIAAVFEEDVGDSESVLLEGSEE 397

[Close]

BLAST of Lsi06G006960 vs. NCBI nr
Match: KAA0038262.1 (seipin-1 [Cucumis melo var. makuwa])

HSP 1 Score: 351.7 bits (901), Expect = 5.3e-93
Identity = 187/239 (78.24%), Postives = 210/239 (87.87%), Query Frame = 0


Query: 13  LSAELISTNGNIITGSSQPCMLRFRSTPVRLTRTFLTSFPLLLGISSESQRLTFPILEHK 72
           LSAELISTNGNIIT SSQPCMLRFRS PVR  RT LTSFP+L+GIS+E+QRL+FPIL+HK
Sbjct: 163 LSAELISTNGNIITSSSQPCMLRFRSAPVRFARTLLTSFPILIGISTEAQRLSFPILKHK 222

Query: 73  EENHQRSAAIHVTISPRIGTTALPELYEAHILINSKLPKTKELLHRWRWTCFLWMSLYLY 132
           EEN +RSAAI  TISPRIGT+ALPELYEAHI+INSKLP+ KELL RWRWTCFLW S+YLY
Sbjct: 223 EENQERSAAIQFTISPRIGTSALPELYEAHIVINSKLPRMKELLRRWRWTCFLWTSIYLY 282

Query: 133 VMFLVIFMFFWKPVVFRAMTLS-ELSGFDEGAPTTKE---AEESFDEMAEITVELLRKWQ 192
           +MFL IFM FWKPV+FRAMTL+ ELS FD+  P  +E   A+ES +EMAEITVELLRKWQ
Sbjct: 283 LMFLAIFMLFWKPVMFRAMTLTPELSDFDQD-PRRRELEGADESLEEMAEITVELLRKWQ 342

Query: 193 EIRRKRKAAVFGYGEVGGGEDDVGSTSASSISCSREYVGAVFEEDVEDSESVLLEGSEE 248
           E+RRKRKAA+FGY   G GE+DVGSTSASSISCSR+Y+ AVFEEDV DSESVLLEGSEE
Sbjct: 343 EMRRKRKAAMFGY---GSGEEDVGSTSASSISCSRDYIAAVFEEDVGDSESVLLEGSEE 397

[Close]

BLAST of Lsi06G006960 vs. TAIR 10
Match: AT5G16460.1 (Putative adipose-regulatory protein (Seipin) )

HSP 1 Score: 128.6 bits (322), Expect = 6.7e-30
Identity = 76/184 (41.30%), Postives = 105/184 (57.07%), Query Frame = 0


Query: 12  QLSAELISTNGNIITGSSQPCMLRFRSTPVRLTRTFLTSFPLLLGISSESQRLTFPILEH 71
           QL  EL+S  G  I  SSQPCMLRFRS P+RL RTF+ S PL+ GI++E+Q +    L+H
Sbjct: 182 QLKVELLSLKGETIARSSQPCMLRFRSKPIRLARTFVMSVPLIAGIANEAQTMRIDALKH 241

Query: 72  KEENHQRSAAIHVTISPRIGTTALPELYEAHILINSKLPKTKELLHRWRWTCFLWMSLYL 131
            +E   R+ A+  T+ PR  T  LP+LYEA I+INSK P  K + + W+WT  +W S+YL
Sbjct: 242 -QEKMPRTKAVRATLIPRAQTRTLPQLYEAEIVINSKPPWIKRMAYNWKWTLCVWTSMYL 301

Query: 132 YVMFLVIFMFFWKPVVFRAMTLSELSGFDEGAPTTKEAEESFDEMAEITVELLRKWQEIR 191
           YV  L   ++ ++PV+F               P T     S  E  EI V    + Q + 
Sbjct: 302 YVAILTALLWCFRPVLF---------------PYTSSRTISESENLEIEVVEEEQEQVME 349

Query: 192 RKRK 196
           R+R+
Sbjct: 362 RRRR 349

[Close]

BLAST of Lsi06G006960 vs. TAIR 10
Match: AT2G34380.1 (Putative adipose-regulatory protein (Seipin) )

HSP 1 Score: 86.3 bits (212), Expect = 3.8e-17
Identity = 45/139 (32.37%), Postives = 82/139 (58.99%), Query Frame = 0


Query: 12  QLSAELISTNGNIITGSSQPCMLRFRSTPVRLTRTFLTSFPLLLGISSESQRLTFPILEH 71
           Q+  + +S +G+++  S +PCM++F S P+RL +T L   PL+ G  SE Q L   +   
Sbjct: 341 QVRVDFLSASGHVLASSRRPCMVKFSSEPIRLVQTLLKIAPLVTGYVSEIQTLNLKLKGL 400

Query: 72  KEENHQRSAAIHVTISPRI---GTTALPELYEAHILINSKLPKTKELLHRWRWTCFLWMS 131
            E++   +A + + I  R        +PE+Y+A + + SKLP  K ++  WR T F+W+S
Sbjct: 401 VEKDIIPTACLKIMIEQRAEFRPGAGIPEIYDASLFLESKLPFLKRIIWNWRKTLFVWIS 460

Query: 132 LYLYVMFLVIFMFFWKPVV 148
           + L++M L+  + F++P++
Sbjct: 461 MSLFIMELLFALVFFRPLI 479

[Close]

BLAST of Lsi06G006960 vs. TAIR 10
Match: AT1G29760.1 (Putative adipose-regulatory protein (Seipin) )

HSP 1 Score: 77.4 bits (189), Expect = 1.8e-14
Identity = 45/139 (32.37%), Postives = 79/139 (56.83%), Query Frame = 0


Query: 12  QLSAELISTNGNIITGSSQPCMLRFRSTPVRLTRTFLTSFPLLLGISSESQRLTFPILEH 71
           Q+  + +S +G  I    +PCMLRFRS P+RL +TF    PL+ G  SE Q L+  +   
Sbjct: 370 QVRVDFLSVDGQTIASIRRPCMLRFRSEPIRLVQTFFKVVPLVTGYVSEIQTLSLKLKGF 429

Query: 72  KEENHQRSAAIHVTISPRI---GTTALPELYEAHILINSKLPKTKELLHRWRWTCFLWMS 131
            E++   +A + + I  R        +PELY+A + + S LP  ++++ +WR T F+W+S
Sbjct: 430 VEKDIP-TACLKIIIEQRAEFRPGAGIPELYDASLSVESGLPFFRKIIWKWRKTLFVWIS 489

Query: 132 LYLYVMFLVIFMFFWKPVV 148
           + L++  L+  +   +P++
Sbjct: 490 MSLFITELLFTLVCCRPLI 507

The following BLAST results are available for this feature:

BLAST of Lsi06G006960 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9FFD9	9.5e-29	41.30	Seipin-1 OS=Arabidopsis thaliana OX=3702 GN=SEI1 PE=2 SV=1	[more]
Q8L615	5.4e-16	32.37	Seipin-3 OS=Arabidopsis thaliana OX=3702 GN=SEI3 PE=2 SV=1	[more]
F4I340	2.5e-13	32.37	Seipin-2 OS=Arabidopsis thaliana OX=3702 GN=SEI2 PE=2 SV=1	[more]

BLAST of Lsi06G006960 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S3B8L6	7.9e-95	77.33	seipin-1 OS=Cucumis melo OX=3656 GN=LOC103487228 PE=4 SV=1	[more]
A0A5D3E501	8.7e-94	78.66	Seipin-1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold84664G00880 PE=...	[more]
A0A5A7T443	2.5e-93	78.24	Seipin-1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold270G001120 PE=4...	[more]
A0A6J1KZV1	9.7e-93	74.58	seipin-1 OS=Cucurbita maxima OX=3661 GN=LOC111498585 PE=4 SV=1	[more]
A0A6J1F5S3	8.2e-92	74.15	seipin-1 OS=Cucurbita moschata OX=3662 GN=LOC111441088 PE=4 SV=1	[more]

BLAST of Lsi06G006960 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038901874.1	1.7e-99	84.30	seipin-1 isoform X2 [Benincasa hispida]	[more]
XP_038901873.1	6.4e-99	83.88	seipin-1 isoform X1 [Benincasa hispida]	[more]
XP_008443693.1	1.6e-94	77.33	PREDICTED: seipin-1 [Cucumis melo]	[more]
TYK30661.1	1.8e-93	78.66	seipin-1 [Cucumis melo var. makuwa]	[more]
KAA0038262.1	5.3e-93	78.24	seipin-1 [Cucumis melo var. makuwa]	[more]

BLAST of Lsi06G006960 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G16460.1	6.7e-30	41.30	Putative adipose-regulatory protein (Seipin)	[more]
AT2G34380.1	3.8e-17	32.37	Putative adipose-regulatory protein (Seipin)	[more]
AT1G29760.1	1.8e-14	32.37	Putative adipose-regulatory protein (Seipin)	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR009617	Seipin family	PFAM	PF06775	Seipin	coord: 12..130 e-value: 1.0E-22 score: 81.1
IPR009617	Seipin family	PANTHER	PTHR21212	BERNARDINELLI-SEIP CONGENITAL LIPODYSTROPHY 2 HOMOLOG BSCL2 PROTEIN	coord: 10..241
None	No IPR available	PANTHER	PTHR21212:SF5	SEIPIN-1	coord: 10..241

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi06G006960.1	Lsi06G006960.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0140042	lipid droplet formation
biological_process	GO:0006629	lipid metabolic process
biological_process	GO:0009846	pollen germination
biological_process	GO:0080155	regulation of double fertilization forming a zygote and endosperm
biological_process	GO:0010162	seed dormancy process
biological_process	GO:0010344	seed oilbody biogenesis
biological_process	GO:0019915	lipid storage
cellular_component	GO:0030176	integral component of endoplasmic reticulum membrane