Lsi06G003400 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi06G003400
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Descriptionsubtilisin-like protease SBT1.8
Locationchr06: 3784647 .. 3788086 (+)
RNA-Seq ExpressionLsi06G003400
SyntenyLsi06G003400
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TTCTTTGTTTGACAGTCAGCTCTGTTTTGAGAGCTCTGTTTCGCCATTTCCCCCCTGCTTTTATGCTTTCTTATAACTAAGAAATTTCTCCTTTCTTCCTTCCCATGGGGTCCATGGCTTCTCATTTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCCTTGTGTCTTCGTGAATGCCAAGAAAACCTACATTGTTCGTATGAAACATAATGCTCTGCCATCACAGTATCTCACCCACCATGACTGGTACTCTGCTCATCTTCAATCTCTCTCTTCATCTTCCCCCTCTGATTCCCTTCTCTATACCTACACTTCCGCCTACCATGGCTTCGCTGCTTCTCTGGATTCAGATGAAGCAGAATTGCTCCGCCAATCCGATTCAGTTCTGGGTGTTTATGAAGACACTGTTTACAATCTTCACACCACTCGAACTCCGGGATTCCTTGGTCTCGACTCCGATTTCGGGTTATGGGAAGGTCACAACACTCAGGATCTCAACCAAGCTTCTCACGATGTCATCATTGGGGTTCTCGATACTGGGATTTGGCCGGAATCAAAGAGCTTTGATGACGCTGGAATGCCGGAGATCCCATCACGGTGGCGTGGAGAATGTGAATCAGGACCTGATTTCAATCCTTCTCTCTGTAACAAGAAACTAATCGGAGCTCGTAGCTTCTCCAAGGGTTATCAAATGGCTTCCGGTGGTGGGTATTTCAGAAAACCTAGAGAAAACGAGTCTCCTCGAGATCAGGACGGCCATGGAACGCACACAGCGAGCACAGCTGCTGGTTCACATGTGGCTAACGCGAGCTTACTTGGTTATGCCAGAGGCATTGCTAGAGGAATGGCTCCTCAAGCAAGAGTTGCTGCTTACAAGACTTGTTGGCCTACTGGTTGCTTTGGATCTGACATTCTTGCCGGTATGGATCGGGCGATATCCGACGGCGTCGATGTACTTTCACTCTCTTTAGGTGGTGGCTCTGCTCCGTATTACAGAGATACCATAGCAATCGGAGCATTTGCAGCCATGGAGAAAGGGGTTTTTGTTTCCTGCTCAGCTGGGAACAGTGGACCAAATAAGGCTTCTCTAGCCAATGTAGCACCGTGGATCATGACAGTCGGAGCTGGAACGCTCGACCGGGACTTCCCAGCTTATGTCCAGCTTGGAAATGGTAAACGGTTCACTGGAGTGTCACTTTACAGTGGGCAAGGAATGGGGAGTAAGCCGGTGGAATTGGTGTACAACAAAGGAAGTAACACCTCTAGCAATATGTGCTTGCCGGGATCTCTCGAGCCGTCGGTGGTTCGGGGGAAGGTGGTGGTTTGTGACAGAGGAATCAATGCCAGAGTAGAAAAGGGAGGAGTGGTGCGAGATGCAGGTGGGATTGGGATGATTCTGGCAAACACAGCAGCTAGTGGGGAGGAATTGGTGGCTGACAGTCACTTGCTGCCAGCCGTGGCGGTGGGCAGGAAAACCGGTGACTTAATCCGGCAGTATGTTCGGTCCATTTCGAATCCAACGGCAGTGCTGAGCTTCGGTGGGACAATTCTGAACGTCCGCCCATCTCCAGTGGTGGCGGCGTTTAGTTCTAGAGGACCCAACTTGGTAACTCCACAAATCCTAAAGCCAGACGTTATTGGTCCTGGCGTTAACATCCTGGCTGCTTGGTCTGAGTCCATTGGACCTACTGGATTGGACAATGACAAGAGGAAAACTCAGTTCAATATTATGTCAGGTATGTCTCAATGGCTAAGGCTCTATCCAATTGTCCTGTGATTTCTAGGCAAATAATGTTCATTCTGTAACTGATAAATCTTAATCTTCTGTTATGTAGGAACTTCCATGTCTTGCCCACATATAAGTGGGTTGGCAGCATTGCTAAAGGCAGCTCATCCACAATGGAGTCCAAGTGCAATCAAATCCGCGTTAATGACAACCGCATACACGCAGGACAACACCAACTCGTCTCTCCGAGATGCGGCCGGAGGGGGATTTTCCAATCCATGGGCTCATGGAGCTGGCCATGTTGATCCTCACAAAGCTCTCTCCCCTGGCCTTCTATACGATATCTCCACTGATGATTACATTGCCTTCTTGTGCTCCTTGGACTATGGGATCGATCATGTTCAGGCAATCGTGAAACGGTCGAACATAACCTGCTCTAGGAAGTTTGCTGATCCAGGACAACTTAACTACCCTTCATTTTCAGTTGTGTTTGGGAGCAAGAGGGTTGTTCGGTACACTCGTATCGTCACCAATGTTGGGGCTGCAGGGTCAGTTTACGAAGTGGCTACTACTGCACCGTCTGTTGTGAATGTGACTGTAAAACCTTCAAAGCTTGTGTTTACTAAAGTTGGGGAGAGGAAGAGGTACACTGTTACATTCGTGGCGAGTAGGGATGCTGCTCAAACCACGAGATTTGGGTTCGGGTCGATTGAGTGGAGCAATGACCAACACCAAGTTAGAAGCCCAGTGGCATTTGCCTGGACAAGGTTGTGATTGATGTTTTCTGTATAGGGCGTTTTCCAATTGTTGTAGGTTTTTCCATTCCTTCAACTTGCTCCAGTTTCAGAAAGAAAACAGCAAGCAAGGTGGTGATCAACAATCAGCAAGAGACTGATCATCATCGAGTATGGCAAAATTTAGGAGGGTTAGTATAAGAGTCTGAAAGGCACCAAGTTGGGAAGTTCTGGGGAGAGTTAAAAAACTAATGTAATGGCATAAAGTTTGGCATTTCTTTTCCTAGTTATCTAAAAATTGTTGTTACTGTCGGGTCTGTTAACGAAAATTCAAAAACGTTGAAGGCCAGTGAAGTGTGTGCTTTTAACTTCGAATGTTGACTCCCAAAATTCTCTCATCTGTGATTTCTCTCTTCATTATAAAACTAGACAAAGCTGCAGTGATAAACATGTTCCTTTCCGTATTTCAACTCACAAACCAAGAAAAGATGACGCTGCACTTTCTCGGCAGAGGTGGTGTGCATAATGAATCAAAACTAGAGACGGATCAAAGTAGCTTACCTGCCAAGCCGATAGGCAAAGGGCCAAAGGATGGAGCATGCAAAGTGTATTGTAGGACCTGTTGATCCTAAACAATGTTTTGCCCGAAACGACCCCCCTTGAAAATGTCACTTTTATGAGGGGGCACTAAAATGGAACTTGATCGGATGCAGGTATCCAATCGTACGCCTGTTGTGTGACCCGTTGGTCCTAAGCATGCAGCGACCCACCTAGAAAAGCTTGCCTTTATGGGGGCACTAAAATGGAACTTGGATCAGACGTGGGTATCCAGTCCCGCCGCAATCATAAAGAACATAAGCACGATACCTCTCAGCCCTTGATGCAAAAGCATCTGGGGCCGTGCTACTAGTACCAGATACAGACCGACACAGATTATCCATAAGCCTCCAGTACGATTACTAATTTA

mRNA sequence

TTCTTTGTTTGACAGTCAGCTCTGTTTTGAGAGCTCTGTTTCGCCATTTCCCCCCTGCTTTTATGCTTTCTTATAACTAAGAAATTTCTCCTTTCTTCCTTCCCATGGGGTCCATGGCTTCTCATTTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCCTTGTGTCTTCGTGAATGCCAAGAAAACCTACATTGTTCGTATGAAACATAATGCTCTGCCATCACAGTATCTCACCCACCATGACTGGTACTCTGCTCATCTTCAATCTCTCTCTTCATCTTCCCCCTCTGATTCCCTTCTCTATACCTACACTTCCGCCTACCATGGCTTCGCTGCTTCTCTGGATTCAGATGAAGCAGAATTGCTCCGCCAATCCGATTCAGTTCTGGGTGTTTATGAAGACACTGTTTACAATCTTCACACCACTCGAACTCCGGGATTCCTTGGTCTCGACTCCGATTTCGGGTTATGGGAAGGTCACAACACTCAGGATCTCAACCAAGCTTCTCACGATGTCATCATTGGGGTTCTCGATACTGGGATTTGGCCGGAATCAAAGAGCTTTGATGACGCTGGAATGCCGGAGATCCCATCACGGTGGCGTGGAGAATGTGAATCAGGACCTGATTTCAATCCTTCTCTCTGTAACAAGAAACTAATCGGAGCTCGTAGCTTCTCCAAGGGTTATCAAATGGCTTCCGGTGGTGGGTATTTCAGAAAACCTAGAGAAAACGAGTCTCCTCGAGATCAGGACGGCCATGGAACGCACACAGCGAGCACAGCTGCTGGTTCACATGTGGCTAACGCGAGCTTACTTGGTTATGCCAGAGGCATTGCTAGAGGAATGGCTCCTCAAGCAAGAGTTGCTGCTTACAAGACTTGTTGGCCTACTGGTTGCTTTGGATCTGACATTCTTGCCGGTATGGATCGGGCGATATCCGACGGCGTCGATGTACTTTCACTCTCTTTAGGTGGTGGCTCTGCTCCGTATTACAGAGATACCATAGCAATCGGAGCATTTGCAGCCATGGAGAAAGGGGTTTTTGTTTCCTGCTCAGCTGGGAACAGTGGACCAAATAAGGCTTCTCTAGCCAATGTAGCACCGTGGATCATGACAGTCGGAGCTGGAACGCTCGACCGGGACTTCCCAGCTTATGTCCAGCTTGGAAATGGTAAACGGTTCACTGGAGTGTCACTTTACAGTGGGCAAGGAATGGGGAGTAAGCCGGTGGAATTGGTGTACAACAAAGGAAGTAACACCTCTAGCAATATGTGCTTGCCGGGATCTCTCGAGCCGTCGGTGGTTCGGGGGAAGGTGGTGGTTTGTGACAGAGGAATCAATGCCAGAGTAGAAAAGGGAGGAGTGGTGCGAGATGCAGGTGGGATTGGGATGATTCTGGCAAACACAGCAGCTAGTGGGGAGGAATTGGTGGCTGACAGTCACTTGCTGCCAGCCGTGGCGGTGGGCAGGAAAACCGGTGACTTAATCCGGCAGTATGTTCGGTCCATTTCGAATCCAACGGCAGTGCTGAGCTTCGGTGGGACAATTCTGAACGTCCGCCCATCTCCAGTGGTGGCGGCGTTTAGTTCTAGAGGACCCAACTTGGTAACTCCACAAATCCTAAAGCCAGACGTTATTGGTCCTGGCGTTAACATCCTGGCTGCTTGGTCTGAGTCCATTGGACCTACTGGATTGGACAATGACAAGAGGAAAACTCAGTTCAATATTATGTCAGGAACTTCCATGTCTTGCCCACATATAAGTGGGTTGGCAGCATTGCTAAAGGCAGCTCATCCACAATGGAGTCCAAGTGCAATCAAATCCGCGTTAATGACAACCGCATACACGCAGGACAACACCAACTCGTCTCTCCGAGATGCGGCCGGAGGGGGATTTTCCAATCCATGGGCTCATGGAGCTGGCCATGTTGATCCTCACAAAGCTCTCTCCCCTGGCCTTCTATACGATATCTCCACTGATGATTACATTGCCTTCTTGTGCTCCTTGGACTATGGGATCGATCATGTTCAGGCAATCGTGAAACGGTCGAACATAACCTGCTCTAGGAAGTTTGCTGATCCAGGACAACTTAACTACCCTTCATTTTCAGTTGTGTTTGGGAGCAAGAGGGTTGTTCGGTACACTCGTATCGTCACCAATGTTGGGGCTGCAGGGTCAGTTTACGAAGTGGCTACTACTGCACCGTCTGTTGTGAATGTGACTGTAAAACCTTCAAAGCTTGTGTTTACTAAAGTTGGGGAGAGGAAGAGGTACACTGTTACATTCGTGGCGAGTAGGGATGCTGCTCAAACCACGAGATTTGGGTTCGGGTCGATTGAGTGGAGCAATGACCAACACCAAGTTAGAAGCCCAGTGGCATTTGCCTGGACAAGGTTGTGATTGATGTTTTCTGTATAGGGCGTTTTCCAATTGTTGTAGGTTTTTCCATTCCTTCAACTTGCTCCAGTTTCAGAAAGAAAACAGCAAGCAAGGTGGTGATCAACAATCAGCAAGAGACTGATCATCATCGAGTATGGCAAAATTTAGGAGGGTTAGTATAAGAGTCTGAAAGGCACCAAGTTGGGAAGTTCTGGGGAGAGTTAAAAAACTAATGTAATGGCATAAAGTTTGGCATTTCTTTTCCTAGTTATCTAAAAATTGTTGTTACTGTCGGGTCTGTTAACGAAAATTCAAAAACGTTGAAGGCCAGTGAAGTGTGTGCTTTTAACTTCGAATGTTGACTCCCAAAATTCTCTCATCTGTGATTTCTCTCTTCATTATAAAACTAGACAAAGCTGCAGTGATAAACATGTTCCTTTCCGTATTTCAACTCACAAACCAAGAAAAGATGACGCTGCACTTTCTCGGCAGAGGTGGTGTGCATAATGAATCAAAACTAGAGACGGATCAAAGTAGCTTACCTGCCAAGCCGATAGGCAAAGGGCCAAAGGATGGAGCATGCAAAGTGTATTGTAGGACCTGTTGATCCTAAACAATGTTTTGCCCGAAACGACCCCCCTTGAAAATGTCACTTTTATGAGGGGGCACTAAAATGGAACTTGATCGGATGCAGGTATCCAATCGTACGCCTGTTGTGTGACCCGTTGGTCCTAAGCATGCAGCGACCCACCTAGAAAAGCTTGCCTTTATGGGGGCACTAAAATGGAACTTGGATCAGACGTGGGTATCCAGTCCCGCCGCAATCATAAAGAACATAAGCACGATACCTCTCAGCCCTTGATGCAAAAGCATCTGGGGCCGTGCTACTAGTACCAGATACAGACCGACACAGATTATCCATAAGCCTCCAGTACGATTACTAATTTA

Coding sequence (CDS)

ATGGGGTCCATGGCTTCTCATTTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCCTTGTGTCTTCGTGAATGCCAAGAAAACCTACATTGTTCGTATGAAACATAATGCTCTGCCATCACAGTATCTCACCCACCATGACTGGTACTCTGCTCATCTTCAATCTCTCTCTTCATCTTCCCCCTCTGATTCCCTTCTCTATACCTACACTTCCGCCTACCATGGCTTCGCTGCTTCTCTGGATTCAGATGAAGCAGAATTGCTCCGCCAATCCGATTCAGTTCTGGGTGTTTATGAAGACACTGTTTACAATCTTCACACCACTCGAACTCCGGGATTCCTTGGTCTCGACTCCGATTTCGGGTTATGGGAAGGTCACAACACTCAGGATCTCAACCAAGCTTCTCACGATGTCATCATTGGGGTTCTCGATACTGGGATTTGGCCGGAATCAAAGAGCTTTGATGACGCTGGAATGCCGGAGATCCCATCACGGTGGCGTGGAGAATGTGAATCAGGACCTGATTTCAATCCTTCTCTCTGTAACAAGAAACTAATCGGAGCTCGTAGCTTCTCCAAGGGTTATCAAATGGCTTCCGGTGGTGGGTATTTCAGAAAACCTAGAGAAAACGAGTCTCCTCGAGATCAGGACGGCCATGGAACGCACACAGCGAGCACAGCTGCTGGTTCACATGTGGCTAACGCGAGCTTACTTGGTTATGCCAGAGGCATTGCTAGAGGAATGGCTCCTCAAGCAAGAGTTGCTGCTTACAAGACTTGTTGGCCTACTGGTTGCTTTGGATCTGACATTCTTGCCGGTATGGATCGGGCGATATCCGACGGCGTCGATGTACTTTCACTCTCTTTAGGTGGTGGCTCTGCTCCGTATTACAGAGATACCATAGCAATCGGAGCATTTGCAGCCATGGAGAAAGGGGTTTTTGTTTCCTGCTCAGCTGGGAACAGTGGACCAAATAAGGCTTCTCTAGCCAATGTAGCACCGTGGATCATGACAGTCGGAGCTGGAACGCTCGACCGGGACTTCCCAGCTTATGTCCAGCTTGGAAATGGTAAACGGTTCACTGGAGTGTCACTTTACAGTGGGCAAGGAATGGGGAGTAAGCCGGTGGAATTGGTGTACAACAAAGGAAGTAACACCTCTAGCAATATGTGCTTGCCGGGATCTCTCGAGCCGTCGGTGGTTCGGGGGAAGGTGGTGGTTTGTGACAGAGGAATCAATGCCAGAGTAGAAAAGGGAGGAGTGGTGCGAGATGCAGGTGGGATTGGGATGATTCTGGCAAACACAGCAGCTAGTGGGGAGGAATTGGTGGCTGACAGTCACTTGCTGCCAGCCGTGGCGGTGGGCAGGAAAACCGGTGACTTAATCCGGCAGTATGTTCGGTCCATTTCGAATCCAACGGCAGTGCTGAGCTTCGGTGGGACAATTCTGAACGTCCGCCCATCTCCAGTGGTGGCGGCGTTTAGTTCTAGAGGACCCAACTTGGTAACTCCACAAATCCTAAAGCCAGACGTTATTGGTCCTGGCGTTAACATCCTGGCTGCTTGGTCTGAGTCCATTGGACCTACTGGATTGGACAATGACAAGAGGAAAACTCAGTTCAATATTATGTCAGGAACTTCCATGTCTTGCCCACATATAAGTGGGTTGGCAGCATTGCTAAAGGCAGCTCATCCACAATGGAGTCCAAGTGCAATCAAATCCGCGTTAATGACAACCGCATACACGCAGGACAACACCAACTCGTCTCTCCGAGATGCGGCCGGAGGGGGATTTTCCAATCCATGGGCTCATGGAGCTGGCCATGTTGATCCTCACAAAGCTCTCTCCCCTGGCCTTCTATACGATATCTCCACTGATGATTACATTGCCTTCTTGTGCTCCTTGGACTATGGGATCGATCATGTTCAGGCAATCGTGAAACGGTCGAACATAACCTGCTCTAGGAAGTTTGCTGATCCAGGACAACTTAACTACCCTTCATTTTCAGTTGTGTTTGGGAGCAAGAGGGTTGTTCGGTACACTCGTATCGTCACCAATGTTGGGGCTGCAGGGTCAGTTTACGAAGTGGCTACTACTGCACCGTCTGTTGTGAATGTGACTGTAAAACCTTCAAAGCTTGTGTTTACTAAAGTTGGGGAGAGGAAGAGGTACACTGTTACATTCGTGGCGAGTAGGGATGCTGCTCAAACCACGAGATTTGGGTTCGGGTCGATTGAGTGGAGCAATGACCAACACCAAGTTAGAAGCCCAGTGGCATTTGCCTGGACAAGGTTGTGA

Protein sequence

MGSMASHFLLLLLLLLLLLLLLPCVFVNAKKTYIVRMKHNALPSQYLTHHDWYSAHLQSLSSSSPSDSLLYTYTSAYHGFAASLDSDEAELLRQSDSVLGVYEDTVYNLHTTRTPGFLGLDSDFGLWEGHNTQDLNQASHDVIIGVLDTGIWPESKSFDDAGMPEIPSRWRGECESGPDFNPSLCNKKLIGARSFSKGYQMASGGGYFRKPRENESPRDQDGHGTHTASTAAGSHVANASLLGYARGIARGMAPQARVAAYKTCWPTGCFGSDILAGMDRAISDGVDVLSLSLGGGSAPYYRDTIAIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKRFTGVSLYSGQGMGSKPVELVYNKGSNTSSNMCLPGSLEPSVVRGKVVVCDRGINARVEKGGVVRDAGGIGMILANTAASGEELVADSHLLPAVAVGRKTGDLIRQYVRSISNPTAVLSFGGTILNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLDNDKRKTQFNIMSGTSMSCPHISGLAALLKAAHPQWSPSAIKSALMTTAYTQDNTNSSLRDAAGGGFSNPWAHGAGHVDPHKALSPGLLYDISTDDYIAFLCSLDYGIDHVQAIVKRSNITCSRKFADPGQLNYPSFSVVFGSKRVVRYTRIVTNVGAAGSVYEVATTAPSVVNVTVKPSKLVFTKVGERKRYTVTFVASRDAAQTTRFGFGSIEWSNDQHQVRSPVAFAWTRL
Homology
BLAST of Lsi06G003400 vs. ExPASy Swiss-Prot
Match: Q9ZUF6 (Subtilisin-like protease SBT1.8 OS=Arabidopsis thaliana OX=3702 GN=SBT1.8 PE=1 SV=1)

HSP 1 Score: 1104.7 bits (2856), Expect = 0.0e+00
Identity = 553/769 (71.91%), Postives = 644/769 (83.75%), Query Frame = 0

Query: 1   MGSMASHFLLLLLLLLLLLLLLPCVFVNAKKTYIVRMKHNALPSQYLTHHDWYSAHLQSL 60
           M S +S    + ++   L LLL      AKKTYI+R+ H+  P  +LTHHDWY++ L S 
Sbjct: 1   MASSSSSSSSITIITTFLFLLL---HTTAKKTYIIRVNHSDKPESFLTHHDWYTSQLNSE 60

Query: 61  SSSSPSDSLLYTYTSAYHGFAASLDSDEAE-LLRQSDSVLGVYEDTVYNLHTTRTPGFLG 120
           S      SLLYTYT+++HGF+A LDS EA+ LL  S+S+L ++ED +Y LHTTRTP FLG
Sbjct: 61  S------SLLYTYTTSFHGFSAYLDSTEADSLLSSSNSILDIFEDPLYTLHTTRTPEFLG 120

Query: 121 LDSDFGLWEGHNTQDLNQASHDVIIGVLDTGIWPESKSFDDAGMPEIPSRWRGECESGPD 180
           L+S+FG+       DL  +S+ VIIGVLDTG+WPES+SFDD  MPEIPS+W+GECESG D
Sbjct: 121 LNSEFGV------HDLGSSSNGVIIGVLDTGVWPESRSFDDTDMPEIPSKWKGECESGSD 180

Query: 181 FNPSLCNKKLIGARSFSKGYQMASGGGYFRKPRENESPRDQDGHGTHTASTAAGSHVANA 240
           F+  LCNKKLIGARSFSKG+QMASGGG+  K RE+ SPRD DGHGTHT++TAAGS V NA
Sbjct: 181 FDSKLCNKKLIGARSFSKGFQMASGGGFSSK-RESVSPRDVDGHGTHTSTTAAGSAVRNA 240

Query: 241 SLLGYARGIARGMAPQARVAAYKTCWPTGCFGSDILAGMDRAISDGVDVLSLSLGGGSAP 300
           S LGYA G ARGMA +ARVA YK CW TGCFGSDILA MDRAI DGVDVLSLSLGGGSAP
Sbjct: 241 SFLGYAAGTARGMATRARVATYKVCWSTGCFGSDILAAMDRAILDGVDVLSLSLGGGSAP 300

Query: 301 YYRDTIAIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQLG 360
           YYRDTIAIGAF+AME+GVFVSCSAGNSGP +AS+ANVAPW+MTVGAGTLDRDFPA+  LG
Sbjct: 301 YYRDTIAIGAFSAMERGVFVSCSAGNSGPTRASVANVAPWVMTVGAGTLDRDFPAFANLG 360

Query: 361 NGKRFTGVSLYSGQGMGSKPVELVYNKGSNTSSNMCLPGSLEPSVVRGKVVVCDRGINAR 420
           NGKR TGVSLYSG GMG+KP+ELVYNKG+++SSN+CLPGSL+ S+VRGK+VVCDRG+NAR
Sbjct: 361 NGKRLTGVSLYSGVGMGTKPLELVYNKGNSSSSNLCLPGSLDSSIVRGKIVVCDRGVNAR 420

Query: 421 VEKGGVVRDAGGIGMILANTAASGEELVADSHLLPAVAVGRKTGDLIRQYVRSISNPTAV 480
           VEKG VVRDAGG+GMI+ANTAASGEELVADSHLLPA+AVG+KTGDL+R+YV+S S PTA+
Sbjct: 421 VEKGAVVRDAGGLGMIMANTAASGEELVADSHLLPAIAVGKKTGDLLREYVKSDSKPTAL 480

Query: 481 LSFGGTILNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLDNDK 540
           L F GT+L+V+PSPVVAAFSSRGPN VTP+ILKPDVIGPGVNILA WS++IGPTGLD D 
Sbjct: 481 LVFKGTVLDVKPSPVVAAFSSRGPNTVTPEILKPDVIGPGVNILAGWSDAIGPTGLDKDS 540

Query: 541 RKTQFNIMSGTSMSCPHISGLAALLKAAHPQWSPSAIKSALMTTAYTQDNTNSSLRDAAG 600
           R+TQFNIMSGTSMSCPHISGLA LLKAAHP+WSPSAIKSALMTTAY  DNTN+ L DAA 
Sbjct: 541 RRTQFNIMSGTSMSCPHISGLAGLLKAAHPEWSPSAIKSALMTTAYVLDNTNAPLHDAAD 600

Query: 601 GGFSNPWAHGAGHVDPHKALSPGLLYDISTDDYIAFLCSLDYGIDHVQAIVKRSNITCSR 660
              SNP+AHG+GHVDP KALSPGL+YDIST++YI FLCSLDY +DH+ AIVKR ++ CS+
Sbjct: 601 NSLSNPYAHGSGHVDPQKALSPGLVYDISTEEYIRFLCSLDYTVDHIVAIVKRPSVNCSK 660

Query: 661 KFADPGQLNYPSFSVVFGSKRVVRYTRIVTNVGAAGSVYEVATTAPSVVNVTVKPSKLVF 720
           KF+DPGQLNYPSFSV+FG KRVVRYTR VTNVGAA SVY+V       V ++VKPSKL F
Sbjct: 661 KFSDPGQLNYPSFSVLFGGKRVVRYTREVTNVGAASSVYKVTVNGAPSVGISVKPSKLSF 720

Query: 721 TKVGERKRYTVTFVASRDAAQTTRFGFGSIEWSNDQHQVRSPVAFAWTR 769
             VGE+KRYTVTFV+ +  + T +  FGSI WSN QH+VRSPVAF+W R
Sbjct: 721 KSVGEKKRYTVTFVSKKGVSMTNKAEFGSITWSNPQHEVRSPVAFSWNR 753

BLAST of Lsi06G003400 vs. ExPASy Swiss-Prot
Match: O65351 (Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana OX=3702 GN=SBT1.7 PE=1 SV=1)

HSP 1 Score: 803.5 bits (2074), Expect = 2.1e-231
Identity = 429/772 (55.57%), Postives = 543/772 (70.34%), Query Frame = 0

Query: 4   MASHFLLLLLLLLLLLLLLPCVFVNA--KKTYIVRMKHNALPSQYLTHHDWYSAHLQSLS 63
           M+S FL      LLL L    V  ++  + TYIV M  + +PS +  H +WY + L+S+S
Sbjct: 1   MSSSFLSSTAFFLLLCLGFCHVSSSSSDQGTYIVHMAKSQMPSSFDLHSNWYDSSLRSIS 60

Query: 64  SSSPSDSLLYTYTSAYHGFAASLDSDEAELLRQSDSVLGVYEDTVYNLHTTRTPGFLGLD 123
            S+    LLYTY +A HGF+  L  +EA+ L     V+ V  +  Y LHTTRTP FLGLD
Sbjct: 61  DSA---ELLYTYENAIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLD 120

Query: 124 SDFGLWEGHNTQDLNQAS---HDVIIGVLDTGIWPESKSFDDAGMPEIPSRWRGECESGP 183
                    +T DL   +    DV++GVLDTG+WPESKS+ D G   IPS W+G CE+G 
Sbjct: 121 --------EHTADLFPEAGSYSDVVVGVLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGT 180

Query: 184 DFNPSLCNKKLIGARSFSKGYQMASGGGYFRKPRENESPRDQDGHGTHTASTAAGSHVAN 243
           +F  SLCN+KLIGAR F++GY+  S  G   + +E+ SPRD DGHGTHT+STAAGS V  
Sbjct: 181 NFTASLCNRKLIGARFFARGYE--STMGPIDESKESRSPRDDDGHGTHTSSTAAGSVVEG 240

Query: 244 ASLLGYARGIARGMAPQARVAAYKTCWPTGCFGSDILAGMDRAISDGVDVLSLSLGGGSA 303
           ASLLGYA G ARGMAP+ARVA YK CW  GCF SDILA +D+AI+D V+VLS+SLGGG +
Sbjct: 241 ASLLGYASGTARGMAPRARVAVYKVCWLGGCFSSDILAAIDKAIADNVNVLSMSLGGGMS 300

Query: 304 PYYRDTIAIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQL 363
            YYRD +AIGAFAAME+G+ VSCSAGN+GP+ +SL+NVAPWI TVGAGTLDRDFPA   L
Sbjct: 301 DYYRDGVAIGAFAAMERGILVSCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAIL 360

Query: 364 GNGKRFTGVSLYSGQGMGSKPVELVY--NKGSNTSSNMCLPGSLEPSVVRGKVVVCDRGI 423
           GNGK FTGVSL+ G+ +  K +  +Y  N  + T+ N+C+ G+L P  V+GK+V+CDRGI
Sbjct: 361 GNGKNFTGVSLFKGEALPDKLLPFIYAGNASNATNGNLCMTGTLIPEKVKGKIVMCDRGI 420

Query: 424 NARVEKGGVVRDAGGIGMILANTAASGEELVADSHLLPAVAVGRKTGDLIRQYVRSISNP 483
           NARV+KG VV+ AGG+GMILANTAA+GEELVAD+HLLPA  VG K GD+IR YV +  NP
Sbjct: 421 NARVQKGDVVKAAGGVGMILANTAANGEELVADAHLLPATTVGEKAGDIIRHYVTTDPNP 480

Query: 484 TAVLSFGGTILNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLD 543
           TA +S  GT++ V+PSPVVAAFSSRGPN +TP ILKPD+I PGVNILAAW+ + GPTGL 
Sbjct: 481 TASISILGTVVGVKPSPVVAAFSSRGPNSITPNILKPDLIAPGVNILAAWTGAAGPTGLA 540

Query: 544 NDKRKTQFNIMSGTSMSCPHISGLAALLKAAHPQWSPSAIKSALMTTAYTQDNTNSSLRD 603
           +D R+ +FNI+SGTSMSCPH+SGLAALLK+ HP+WSP+AI+SALMTTAY        L D
Sbjct: 541 SDSRRVEFNIISGTSMSCPHVSGLAALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLD 600

Query: 604 AAGGGFSNPWAHGAGHVDPHKALSPGLLYDISTDDYIAFLCSLDYGIDHVQAIVKRSNIT 663
            A G  S P+ HGAGHV P  A +PGL+YD++T+DY+ FLC+L+Y    +++ V R N T
Sbjct: 601 IATGKPSTPFDHGAGHVSPTTATNPGLIYDLTTEDYLGFLCALNYTSPQIRS-VSRRNYT 660

Query: 664 CS-RKFADPGQLNYPSFSVVFGSKRVVRYTRIVTNVGAAGSVYEVATTAPSVVNVTVKPS 723
           C   K      LNYPSF+V        +YTR VT+VG AG+     T+  + V ++V+P+
Sbjct: 661 CDPSKSYSVADLNYPSFAVNVDGVGAYKYTRTVTSVGGAGTYSVKVTSETTGVKISVEPA 720

Query: 724 KLVFTKVGERKRYTVTFVASRDAAQTTRFGFGSIEWSNDQHQVRSPVAFAWT 768
            L F +  E+K YTVTF         +   FGSIEWS+ +H V SPVA +WT
Sbjct: 721 VLNFKEANEKKSYTVTFTVDSSKPSGSN-SFGSIEWSDGKHVVGSPVAISWT 757

BLAST of Lsi06G003400 vs. ExPASy Swiss-Prot
Match: Q9LVJ1 (Subtilisin-like protease SBT1.4 OS=Arabidopsis thaliana OX=3702 GN=SBT1.4 PE=2 SV=1)

HSP 1 Score: 758.8 bits (1958), Expect = 5.8e-218
Identity = 413/774 (53.36%), Postives = 520/774 (67.18%), Query Frame = 0

Query: 10  LLLLLLLLLLLLLPCVF------VNAKKTYIVRMKHNALPSQYLTHHDWYSAHLQSLSSS 69
           L L  +  +  LL C F       +  ++YIV ++ +  PS + +H++W+ + L+SL SS
Sbjct: 4   LSLSSIFFVFPLLLCFFSPSSSSSDGLESYIVHVQRSHKPSLFSSHNNWHVSLLRSLPSS 63

Query: 70  SPSDSLLYTYTSAYHGFAASLDSDEAELLRQSDSVLGVYEDTVYNLHTTRTPGFLGLDSD 129
               +LLY+Y+ A HGF+A L   +   LR+  SV+ V  D    +HTT TP FLG   +
Sbjct: 64  PQPATLLYSYSRAVHGFSARLSPIQTAALRRHPSVISVIPDQAREIHTTHTPAFLGFSQN 123

Query: 130 FGLWEGHNTQDLNQASHDVIIGVLDTGIWPESKSFDDAGMPEIPSRWRGECESGPDFNPS 189
            GLW   N         DVI+GVLDTGIWPE  SF D+G+  IPS W+GECE GPDF  S
Sbjct: 124 SGLWSNSN------YGEDVIVGVLDTGIWPEHPSFSDSGLGPIPSTWKGECEIGPDFPAS 183

Query: 190 LCNKKLIGARSFSKGYQMASGGGYFRKPRENESPRDQDGHGTHTASTAAGSHVANASLLG 249
            CN+KLIGAR+F +GY     G      +E+ SPRD +GHGTHTASTAAGS VANASL  
Sbjct: 184 SCNRKLIGARAFYRGYLTQRNGTKKHAAKESRSPRDTEGHGTHTASTAAGSVVANASLYQ 243

Query: 250 YARGIARGMAPQARVAAYKTCWPTGCFGSDILAGMDRAISDGVDVLSLSLG-GGSAP-YY 309
           YARG A GMA +AR+AAYK CW  GC+ SDILA MD+A++DGV V+SLS+G  GSAP Y+
Sbjct: 244 YARGTATGMASKARIAAYKICWTGGCYDSDILAAMDQAVADGVHVISLSVGASGSAPEYH 303

Query: 310 RDTIAIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQLGNG 369
            D+IAIGAF A   G+ VSCSAGNSGPN  +  N+APWI+TVGA T+DR+F A    G+G
Sbjct: 304 TDSIAIGAFGATRHGIVVSCSAGNSGPNPETATNIAPWILTVGASTVDREFAANAITGDG 363

Query: 370 KRFTGVSLYSGQGMGSKPVELVYNKGSNTSSNMCLPGSLEPSVVRGKVVVCDRGINARVE 429
           K FTG SLY+G+ +    + LVY+   +  S +C PG L  S+V GK+V+CDRG NARVE
Sbjct: 364 KVFTGTSLYAGESLPDSQLSLVYS--GDCGSRLCYPGKLNSSLVEGKIVLCDRGGNARVE 423

Query: 430 KGGVVRDAGGIGMILANTAASGEELVADSHLLPAVAVGRKTGDLIRQYVRSISNPTAVLS 489
           KG  V+ AGG GMILANTA SGEEL ADSHL+PA  VG K GD IR Y+++  +PTA +S
Sbjct: 424 KGSAVKLAGGAGMILANTAESGEELTADSHLVPATMVGAKAGDQIRDYIKTSDSPTAKIS 483

Query: 490 FGGTILNVR-PSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLDNDKR 549
           F GT++    PSP VAAFSSRGPN +TP ILKPDVI PGVNILA W+  +GPT LD D R
Sbjct: 484 FLGTLIGPSPPSPRVAAFSSRGPNHLTPVILKPDVIAPGVNILAGWTGMVGPTDLDIDPR 543

Query: 550 KTQFNIMSGTSMSCPHISGLAALLKAAHPQWSPSAIKSALMTTAYTQDNTNSSLRDAAGG 609
           + QFNI+SGTSMSCPH+SGLAALL+ AHP WSP+AIKSAL+TTAY  +N+   + D A G
Sbjct: 544 RVQFNIISGTSMSCPHVSGLAALLRKAHPDWSPAAIKSALVTTAYDVENSGEPIEDLATG 603

Query: 610 GFSNPWAHGAGHVDPHKALSPGLLYDISTDDYIAFLCSLDYGIDHVQAIVKRSNI--TC- 669
             SN + HGAGHVDP+KAL+PGL+YDI   +Y+AFLC++ Y    +   ++   +   C 
Sbjct: 604 KSSNSFIHGAGHVDPNKALNPGLVYDIEVKEYVAFLCAVGYEFPGILVFLQDPTLYDACE 663

Query: 670 SRKFADPGQLNYPSFSVVFGSK-RVVRYTRIVTNVGA-AGSVYEVATTAPSVVNVTVKPS 729
           + K    G LNYPSFSVVF S   VV+Y R+V NVG+   +VYEV   +P+ V + V PS
Sbjct: 664 TSKLRTAGDLNYPSFSVVFASTGEVVKYKRVVKNVGSNVDAVYEVGVKSPANVEIDVSPS 723

Query: 730 KLVFTKVGERKRYTVTF---VASRDAAQTTRFGFGSIEWSNDQHQVRSPVAFAW 767
           KL F+K      Y VTF   V            FGSIEW++ +H V+SPVA  W
Sbjct: 724 KLAFSKEKSVLEYEVTFKSVVLGGGVGSVPGHEFGSIEWTDGEHVVKSPVAVQW 769

BLAST of Lsi06G003400 vs. ExPASy Swiss-Prot
Match: Q9FLI4 (Subtilisin-like protease SBT1.3 OS=Arabidopsis thaliana OX=3702 GN=SBT1.3 PE=2 SV=1)

HSP 1 Score: 738.8 bits (1906), Expect = 6.2e-212
Identity = 391/775 (50.45%), Postives = 514/775 (66.32%), Query Frame = 0

Query: 8   FLLLLLLLLLLLLLLPCVF-VNAKKTYIVRMKHNALPSQYLTHHDWYSAHLQSLSSSSPS 67
           FL ++L + L+ L       ++ KKTY++ M  +A+P  Y  H  WYS+ + S++     
Sbjct: 11  FLFIILSINLIFLQAETTTQISTKKTYVIHMDKSAMPLPYTNHLQWYSSKINSVTQHKSQ 70

Query: 68  DS------LLYTYTSAYHGFAASLDSDEAELLRQSDSVLGVYEDTVYNLHTTRTPGFLGL 127
           +       +LYTY +A+HG AA L  +EAE L + D V+ V  +T Y LHTTR+P FLGL
Sbjct: 71  EEEGNNNRILYTYQTAFHGLAAQLTQEEAERLEEEDGVVAVIPETRYELHTTRSPTFLGL 130

Query: 128 DSDFGLWEGHNTQDLNQASHDVIIGVLDTGIWPESKSFDDAGMPEIPSRWRGECESGPDF 187
           +      E           HDV++GVLDTGIWPES+SF+D GM  +P+ WRG CE+G  F
Sbjct: 131 ERQ----ESERVWAERVTDHDVVVGVLDTGIWPESESFNDTGMSPVPATWRGACETGKRF 190

Query: 188 NPSLCNKKLIGARSFSKGYQMASGGGYFRKPRENESPRDQDGHGTHTASTAAGSHVANAS 247
               CN+K++GAR F +GY+ A+  G   +  E +SPRD+DGHGTHTA+T AGS V  A+
Sbjct: 191 LKRNCNRKIVGARVFYRGYEAAT--GKIDEELEYKSPRDRDGHGTHTAATVAGSPVKGAN 250

Query: 248 LLGYARGIARGMAPQARVAAYKTCWPTGCFGSDILAGMDRAISDGVDVLSLSLGGGSAPY 307
           L G+A G ARGMA +ARVAAYK CW  GCF SDIL+ +D+A++DGV VLS+SLGGG + Y
Sbjct: 251 LFGFAYGTARGMAQKARVAAYKVCWVGGCFSSDILSAVDQAVADGVQVLSISLGGGVSTY 310

Query: 308 YRDTIAIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQLGN 367
            RD+++I  F AME GVFVSCSAGN GP+  SL NV+PWI TVGA T+DRDFPA V++G 
Sbjct: 311 SRDSLSIATFGAMEMGVFVSCSAGNGGPDPISLTNVSPWITTVGASTMDRDFPATVKIGT 370

Query: 368 GKRFTGVSLYSGQGM--GSKPVELVY---NKGSNTSSNMCLPGSLEPSVVRGKVVVCDRG 427
            + F GVSLY G+ +   +K   LVY   N  S   ++ CL G+L+   V GK+V+CDRG
Sbjct: 371 MRTFKGVSLYKGRTVLPKNKQYPLVYLGRNASSPDPTSFCLDGALDRRHVAGKIVICDRG 430

Query: 428 INARVEKGGVVRDAGGIGMILANTAASGEELVADSHLLPAVAVGRKTGDLIRQYVRSISN 487
           +  RV+KG VV+ AGGIGM+L NTA +GEELVADSH+LPAVAVG K G LI+QY  +   
Sbjct: 431 VTPRVQKGQVVKRAGGIGMVLTNTATNGEELVADSHMLPAVAVGEKEGKLIKQYAMTSKK 490

Query: 488 PTAVLSFGGTILNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGL 547
            TA L   GT + ++PSPVVAAFSSRGPN ++ +ILKPD++ PGVNILAAW+  + P+ L
Sbjct: 491 ATASLEILGTRIGIKPSPVVAAFSSRGPNFLSLEILKPDLLAPGVNILAAWTGDMAPSSL 550

Query: 548 DNDKRKTQFNIMSGTSMSCPHISGLAALLKAAHPQWSPSAIKSALMTTAYTQDNTNSSLR 607
            +D R+ +FNI+SGTSMSCPH+SG+AAL+K+ HP WSP+AIKSALMTTAY  DN    L 
Sbjct: 551 SSDPRRVKFNILSGTSMSCPHVSGVAALIKSRHPDWSPAAIKSALMTTAYVHDNMFKPLT 610

Query: 608 DAAGGGFSNPWAHGAGHVDPHKALSPGLLYDISTDDYIAFLCSLDYGIDHVQAIVKRSNI 667
           DA+G   S+P+ HGAGH+DP +A  PGL+YDI   +Y  FLC+ D     ++   K SN 
Sbjct: 611 DASGAAPSSPYDHGAGHIDPLRATDPGLVYDIGPQEYFEFLCTQDLSPSQLKVFTKHSNR 670

Query: 668 TCSRKFA-DPGQLNYPSFSVVFGSK---RVVRYTRIVTNVGAAGSVYEVATTAPSVVNVT 727
           TC    A +PG LNYP+ S +F      + +   R VTNVG   S Y+V+ +     +VT
Sbjct: 671 TCKHTLAKNPGNLNYPAISALFPENTHVKAMTLRRTVTNVGPHISSYKVSVSPFKGASVT 730

Query: 728 VKPSKLVFTKVGERKRYTVTFVASRDAAQTTRFGFGSIEWSNDQHQVRSPVAFAW 767
           V+P  L FT   ++  YTVTF   R   +  R  FG + W +  H+VRSPV   W
Sbjct: 731 VQPKTLNFTSKHQKLSYTVTF---RTRFRMKRPEFGGLVWKSTTHKVRSPVIITW 776

BLAST of Lsi06G003400 vs. ExPASy Swiss-Prot
Match: Q9LUM3 (Subtilisin-like protease SBT1.5 OS=Arabidopsis thaliana OX=3702 GN=SBT1.5 PE=2 SV=1)

HSP 1 Score: 734.6 bits (1895), Expect = 1.2e-210
Identity = 384/749 (51.27%), Postives = 511/749 (68.22%), Query Frame = 0

Query: 32  TYIVRMKHNALPSQYLTHHDWYSAHLQSLSSSSPSDSLLYTYTSAYHGFAASLDSDEAEL 91
           TYIV + H A PS + TH  WY++ L SL+SS P  S+++TY + +HGF+A L S +A  
Sbjct: 27  TYIVHVDHEAKPSIFPTHFHWYTSSLASLTSSPP--SIIHTYDTVFHGFSARLTSQDASQ 86

Query: 92  LRQSDSVLGVYEDTVYNLHTTRTPGFLGLDSDFGLWEGHNTQDLNQASHDVIIGVLDTGI 151
           L     V+ V  + V +LHTTR+P FLGL S     +     + +    D++IGV+DTG+
Sbjct: 87  LLDHPHVISVIPEQVRHLHTTRSPEFLGLRST----DKAGLLEESDFGSDLVIGVIDTGV 146

Query: 152 WPESKSFDDAGMPEIPSRWRGECESGPDFNPSLCNKKLIGARSFSKGYQMASGGGYFRKP 211
           WPE  SFDD G+  +P +W+G+C +  DF  S CN+KL+GAR F  GY+  +  G   + 
Sbjct: 147 WPERPSFDDRGLGPVPIKWKGQCIASQDFPESACNRKLVGARFFCGGYEATN--GKMNET 206

Query: 212 RENESPRDQDGHGTHTASTAAGSHVANASLLGYARGIARGMAPQARVAAYKTCWPTGCFG 271
            E  SPRD DGHGTHTAS +AG +V  AS LGYA G+A GMAP+AR+AAYK CW +GC+ 
Sbjct: 207 TEFRSPRDSDGHGTHTASISAGRYVFPASTLGYAHGVAAGMAPKARLAAYKVCWNSGCYD 266

Query: 272 SDILAGMDRAISDGVDVLSLSLGGGSAPYYRDTIAIGAFAAMEKGVFVSCSAGNSGPNKA 331
           SDILA  D A++DGVDV+SLS+GG   PYY D IAIGAF A+++G+FVS SAGN GP   
Sbjct: 267 SDILAAFDTAVADGVDVISLSVGGVVVPYYLDAIAIGAFGAIDRGIFVSASAGNGGPGAL 326

Query: 332 SLANVAPWIMTVGAGTLDRDFPAYVQLGNGKRFTGVSLYSGQGMG-SKPVELVYN----K 391
           ++ NVAPW+ TVGAGT+DRDFPA V+LGNGK  +GVS+Y G G+   +   LVY      
Sbjct: 327 TVTNVAPWMTTVGAGTIDRDFPANVKLGNGKMISGVSVYGGPGLDPGRMYPLVYGGSLLG 386

Query: 392 GSNTSSNMCLPGSLEPSVVRGKVVVCDRGINARVEKGGVVRDAGGIGMILANTAASGEEL 451
           G   SS++CL GSL+P++V+GK+V+CDRGIN+R  KG +VR  GG+GMI+AN    GE L
Sbjct: 387 GDGYSSSLCLEGSLDPNLVKGKIVLCDRGINSRATKGEIVRKNGGLGMIIANGVFDGEGL 446

Query: 452 VADSHLLPAVAVGRKTGDLIRQYV------RSISNPTAVLSFGGTILNVRPSPVVAAFSS 511
           VAD H+LPA +VG   GD IR+Y+      RS  +PTA + F GT L +RP+PVVA+FS+
Sbjct: 447 VADCHVLPATSVGASGGDEIRRYISESSKSRSSKHPTATIVFKGTRLGIRPAPVVASFSA 506

Query: 512 RGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLDNDKRKTQFNIMSGTSMSCPHISGL 571
           RGPN  TP+ILKPDVI PG+NILAAW + IGP+G+ +D R+T+FNI+SGTSM+CPH+SGL
Sbjct: 507 RGPNPETPEILKPDVIAPGLNILAAWPDRIGPSGVTSDNRRTEFNILSGTSMACPHVSGL 566

Query: 572 AALLKAAHPQWSPSAIKSALMTTAYTQDNTNSSLRDAAGGGFSNPWAHGAGHVDPHKALS 631
           AALLKAAHP WSP+AI+SAL+TTAYT DN+   + D + G  S+   +G+GHV P KA+ 
Sbjct: 567 AALLKAAHPDWSPAAIRSALITTAYTVDNSGEPMMDESTGNTSSVMDYGSGHVHPTKAMD 626

Query: 632 PGLLYDISTDDYIAFLCSLDYGIDHVQAIVKR-SNITCSRKFADPGQLNYPSFSVVF--- 691
           PGL+YDI++ DYI FLC+ +Y   ++  I +R ++   +R+    G LNYPSFSVVF   
Sbjct: 627 PGLVYDITSYDYINFLCNSNYTRTNIVTITRRQADCDGARRAGHVGNLNYPSFSVVFQQY 686

Query: 692 -GSKRVVRYTRIVTNVGAAGSVYEVATTAPSVVNVTVKPSKLVFTKVGERKRYTVTFVAS 751
             SK    + R VTNVG + SVYE+    P    VTV+P KL F +VG++  + V    +
Sbjct: 687 GESKMSTHFIRTVTNVGDSDSVYEIKIRPPRGTTVTVEPEKLSFRRVGQKLSFVVRVKTT 746

Query: 752 --RDAAQTTRFGFGSIEWSNDQHQVRSPV 763
             + +   T    G I WS+ +  V SP+
Sbjct: 747 EVKLSPGATNVETGHIVWSDGKRNVTSPL 767

BLAST of Lsi06G003400 vs. ExPASy TrEMBL
Match: A0A6J1JIF3 (subtilisin-like protease SBT1.8 OS=Cucurbita maxima OX=3661 GN=LOC111484737 PE=3 SV=1)

HSP 1 Score: 1447.6 bits (3746), Expect = 0.0e+00
Identity = 728/769 (94.67%), Postives = 747/769 (97.14%), Query Frame = 0

Query: 1   MGSMASHFLLLLLLLLLLLLLLPCVFVNAKKTYIVRMKHNALPSQYLTHHDWYSAHLQSL 60
           MGSMA    LL+   LLLLLLLPCVFVNAK+TYIVRMKH+ALPS YLTHHDWYSAHLQSL
Sbjct: 1   MGSMAR---LLVASFLLLLLLLPCVFVNAKRTYIVRMKHHALPSDYLTHHDWYSAHLQSL 60

Query: 61  SSSSPSDSLLYTYTSAYHGFAASLDSDEAELLRQSDSVLGVYEDTVYNLHTTRTPGFLGL 120
           SSS+ SDSLLYTYTSAYHGFAASLD DEAELLRQSDSVLGVYEDTVY LHTTRTPGFLGL
Sbjct: 61  SSSATSDSLLYTYTSAYHGFAASLDPDEAELLRQSDSVLGVYEDTVYKLHTTRTPGFLGL 120

Query: 121 DSDFGLWEGHNTQDLNQASHDVIIGVLDTGIWPESKSFDDAGMPEIPSRWRGECESGPDF 180
           DSDFGLWEGHNTQDL+QASHDVIIGVLDTGIWPESKSFDD GMPEIPSRWRGECESGPDF
Sbjct: 121 DSDFGLWEGHNTQDLDQASHDVIIGVLDTGIWPESKSFDDTGMPEIPSRWRGECESGPDF 180

Query: 181 NPSLCNKKLIGARSFSKGYQMASGGGYFRKPRENESPRDQDGHGTHTASTAAGSHVANAS 240
           +PSLCNKKLIGARSFSKGYQMASGGGYFRK RENESPRDQDGHGTHTASTAAGSHVANAS
Sbjct: 181 SPSLCNKKLIGARSFSKGYQMASGGGYFRKSRENESPRDQDGHGTHTASTAAGSHVANAS 240

Query: 241 LLGYARGIARGMAPQARVAAYKTCWPTGCFGSDILAGMDRAISDGVDVLSLSLGGGSAPY 300
           LLGYARGIARGMAPQARVAAYKTCWPTGCFGSDILAGMDRAISDGVDVLSLSLGGGSAPY
Sbjct: 241 LLGYARGIARGMAPQARVAAYKTCWPTGCFGSDILAGMDRAISDGVDVLSLSLGGGSAPY 300

Query: 301 YRDTIAIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQLGN 360
           YRDTIAIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQLGN
Sbjct: 301 YRDTIAIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQLGN 360

Query: 361 GKRFTGVSLYSGQGMGSKPVELVYNKGSNTSSNMCLPGSLEPSVVRGKVVVCDRGINARV 420
           GKRFTGVSLYSGQGMG+KPV LVYNKGSN+SSNMCLPGSLEP+ VRGKVV+CDRGINARV
Sbjct: 361 GKRFTGVSLYSGQGMGNKPVALVYNKGSNSSSNMCLPGSLEPAFVRGKVVICDRGINARV 420

Query: 421 EKGGVVRDAGGIGMILANTAASGEELVADSHLLPAVAVGRKTGDLIRQYVRSISNPTAVL 480
           EKGGVVRDAGGIGMILANTAASGEELVADSHLLPAVAVG KTGDLIRQYVRS++ PTAVL
Sbjct: 421 EKGGVVRDAGGIGMILANTAASGEELVADSHLLPAVAVGAKTGDLIRQYVRSVAKPTAVL 480

Query: 481 SFGGTILNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLDNDKR 540
           SFGGT+LNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLDNDKR
Sbjct: 481 SFGGTVLNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLDNDKR 540

Query: 541 KTQFNIMSGTSMSCPHISGLAALLKAAHPQWSPSAIKSALMTTAYTQDNTNSSLRDAAGG 600
           KTQFNIMSGTSMSCPHISGLAALLKAAHP WSPSAIKSALMTTAYTQDNTNSSLRDAAGG
Sbjct: 541 KTQFNIMSGTSMSCPHISGLAALLKAAHPSWSPSAIKSALMTTAYTQDNTNSSLRDAAGG 600

Query: 601 GFSNPWAHGAGHVDPHKALSPGLLYDISTDDYIAFLCSLDYGIDHVQAIVKRSNITCSRK 660
            FSNPWAHGAGHVDPHKALSPGLLYDIST+DYI FLCSLDYGIDHVQAIVKRSNITCS+K
Sbjct: 601 MFSNPWAHGAGHVDPHKALSPGLLYDISTNDYITFLCSLDYGIDHVQAIVKRSNITCSKK 660

Query: 661 FADPGQLNYPSFSVVFGSKRVVRYTRIVTNVGAAGSVYEVATTAPSVVNVTVKPSKLVFT 720
           FADPGQLNYPSFSVVFGSKRVVRYTRIVTNVGAAGSVYEVATTAPSVV VTVKPSKLVF+
Sbjct: 661 FADPGQLNYPSFSVVFGSKRVVRYTRIVTNVGAAGSVYEVATTAPSVVKVTVKPSKLVFS 720

Query: 721 KVGERKRYTVTFVASRDAAQTTRFGFGSIEWSNDQHQVRSPVAFAWTRL 770
           KVGERKRYTVTFVASRDAA+TTR+GFGSI WSNDQHQVRSPVAFAWT+L
Sbjct: 721 KVGERKRYTVTFVASRDAARTTRYGFGSIAWSNDQHQVRSPVAFAWTKL 766

BLAST of Lsi06G003400 vs. ExPASy TrEMBL
Match: A0A6J1H8F1 (subtilisin-like protease SBT1.8 OS=Cucurbita moschata OX=3662 GN=LOC111461488 PE=3 SV=1)

HSP 1 Score: 1446.0 bits (3742), Expect = 0.0e+00
Identity = 726/769 (94.41%), Postives = 746/769 (97.01%), Query Frame = 0

Query: 1   MGSMASHFLLLLLLLLLLLLLLPCVFVNAKKTYIVRMKHNALPSQYLTHHDWYSAHLQSL 60
           MGSMA    LL+   LLLLLLLPCVFVNAK+TYIVRMKH+ALPS YLTHHDWYSAHLQSL
Sbjct: 1   MGSMAR---LLVASFLLLLLLLPCVFVNAKRTYIVRMKHHALPSDYLTHHDWYSAHLQSL 60

Query: 61  SSSSPSDSLLYTYTSAYHGFAASLDSDEAELLRQSDSVLGVYEDTVYNLHTTRTPGFLGL 120
           SSS+ SDSLLYTYTSAYHGFAASLD DEAELLRQSDSVLGVYEDTVYNLHTTRTPGFLGL
Sbjct: 61  SSSATSDSLLYTYTSAYHGFAASLDPDEAELLRQSDSVLGVYEDTVYNLHTTRTPGFLGL 120

Query: 121 DSDFGLWEGHNTQDLNQASHDVIIGVLDTGIWPESKSFDDAGMPEIPSRWRGECESGPDF 180
           DSDFGLWEGHNTQDL+QASHDVIIGVLDTGIWPESKSFDD GMPEIPSRWRGECESGPDF
Sbjct: 121 DSDFGLWEGHNTQDLDQASHDVIIGVLDTGIWPESKSFDDTGMPEIPSRWRGECESGPDF 180

Query: 181 NPSLCNKKLIGARSFSKGYQMASGGGYFRKPRENESPRDQDGHGTHTASTAAGSHVANAS 240
           +PSLCNKKLIGARSFSKGYQMASGGGYFRK RENESPRDQDGHGTHTASTAAGSHVANAS
Sbjct: 181 SPSLCNKKLIGARSFSKGYQMASGGGYFRKSRENESPRDQDGHGTHTASTAAGSHVANAS 240

Query: 241 LLGYARGIARGMAPQARVAAYKTCWPTGCFGSDILAGMDRAISDGVDVLSLSLGGGSAPY 300
           LLGYARGIARGMAPQARVAAYKTCWPTGCFGSDILAGMDRAISDGVDVLSLSLGGGSAPY
Sbjct: 241 LLGYARGIARGMAPQARVAAYKTCWPTGCFGSDILAGMDRAISDGVDVLSLSLGGGSAPY 300

Query: 301 YRDTIAIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQLGN 360
           YRDTIAIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQLGN
Sbjct: 301 YRDTIAIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQLGN 360

Query: 361 GKRFTGVSLYSGQGMGSKPVELVYNKGSNTSSNMCLPGSLEPSVVRGKVVVCDRGINARV 420
           GKRFTGVSLYSGQGMG+KPV LVYNKGSN+SSNMCLPGSLEP+ VRGKVV+CDRGINARV
Sbjct: 361 GKRFTGVSLYSGQGMGNKPVALVYNKGSNSSSNMCLPGSLEPAFVRGKVVICDRGINARV 420

Query: 421 EKGGVVRDAGGIGMILANTAASGEELVADSHLLPAVAVGRKTGDLIRQYVRSISNPTAVL 480
           EKGGVVRDAGGIGMILANTAASGEELVADSHLLPAVAVG KTGDLIRQYVRS++ PTAVL
Sbjct: 421 EKGGVVRDAGGIGMILANTAASGEELVADSHLLPAVAVGAKTGDLIRQYVRSVAKPTAVL 480

Query: 481 SFGGTILNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLDNDKR 540
           SFGGT+LNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLDNDKR
Sbjct: 481 SFGGTVLNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLDNDKR 540

Query: 541 KTQFNIMSGTSMSCPHISGLAALLKAAHPQWSPSAIKSALMTTAYTQDNTNSSLRDAAGG 600
           KTQFNIMSGTSMSCPHISGLAALLKAAHP WSPSAIKSALMTTAYTQDNTNSSLRDAAGG
Sbjct: 541 KTQFNIMSGTSMSCPHISGLAALLKAAHPSWSPSAIKSALMTTAYTQDNTNSSLRDAAGG 600

Query: 601 GFSNPWAHGAGHVDPHKALSPGLLYDISTDDYIAFLCSLDYGIDHVQAIVKRSNITCSRK 660
            FSNPWAHGAGHVDPHKALSPGL+YDIST+DYI FLCSLDYGIDHVQAI KRSNITC +K
Sbjct: 601 AFSNPWAHGAGHVDPHKALSPGLVYDISTNDYITFLCSLDYGIDHVQAIAKRSNITCLKK 660

Query: 661 FADPGQLNYPSFSVVFGSKRVVRYTRIVTNVGAAGSVYEVATTAPSVVNVTVKPSKLVFT 720
           FADPGQLNYPSFSVVFGSKRVVRYTRIVTNVGAAGSVYEVATTAPSVV VTVKPSKLVF+
Sbjct: 661 FADPGQLNYPSFSVVFGSKRVVRYTRIVTNVGAAGSVYEVATTAPSVVKVTVKPSKLVFS 720

Query: 721 KVGERKRYTVTFVASRDAAQTTRFGFGSIEWSNDQHQVRSPVAFAWTRL 770
           KVGERKRYTVTFVASRDAA+TTR+GFGSI WSNDQHQVRSPVAFAWT+L
Sbjct: 721 KVGERKRYTVTFVASRDAARTTRYGFGSIAWSNDQHQVRSPVAFAWTKL 766

BLAST of Lsi06G003400 vs. ExPASy TrEMBL
Match: A0A5A7UKV3 (Subtilisin-like protease SBT1.8 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold181G001560 PE=3 SV=1)

HSP 1 Score: 1440.2 bits (3727), Expect = 0.0e+00
Identity = 723/758 (95.38%), Postives = 740/758 (97.63%), Query Frame = 0

Query: 12  LLLLLLLLLLLPCVFVNAKKTYIVRMKHNALPSQYLTHHDWYSAHLQSLSSSSPSDSLLY 71
           L+  LLLLLLL CVF+NAKKTYIV MKH+ALPS+YLTHHDWYSA LQSLSSSS SDSLLY
Sbjct: 8   LIPFLLLLLLLSCVFINAKKTYIVHMKHHALPSEYLTHHDWYSASLQSLSSSSSSDSLLY 67

Query: 72  TYTSAYHGFAASLDSDEAELLRQSDSVLGVYEDTVYNLHTTRTPGFLGLDSDFGLWEGHN 131
           TYTS++HGFAA LDS+E ELLRQSDSVLGVYEDTVYNLHTTRTPGFLGLDSDFGLWEGH 
Sbjct: 68  TYTSSFHGFAAFLDSEEVELLRQSDSVLGVYEDTVYNLHTTRTPGFLGLDSDFGLWEGHT 127

Query: 132 TQDLNQASHDVIIGVLDTGIWPESKSFDDAGMPEIPSRWRGECESGPDFNPSLCNKKLIG 191
           TQDLNQASHDVIIGVLDTGIWPESKSFDD GMPEIPSRWRG CE+GPDF+PSLCNKKLIG
Sbjct: 128 TQDLNQASHDVIIGVLDTGIWPESKSFDDTGMPEIPSRWRGGCEAGPDFSPSLCNKKLIG 187

Query: 192 ARSFSKGYQMASGGGYFRKPRENESPRDQDGHGTHTASTAAGSHVANASLLGYARGIARG 251
           ARSFSKGYQMASGGGYFRKPREN+S RDQDGHGTHTASTAAGSHV NASLLGYARGIARG
Sbjct: 188 ARSFSKGYQMASGGGYFRKPRENQSARDQDGHGTHTASTAAGSHVPNASLLGYARGIARG 247

Query: 252 MAPQARVAAYKTCWPTGCFGSDILAGMDRAISDGVDVLSLSLGGGSAPYYRDTIAIGAFA 311
           MAPQARVAAYKTCWPTGCFGSDILAGMDRAISDGVDVLSLSLGGGSAPYYRDTIAIGAFA
Sbjct: 248 MAPQARVAAYKTCWPTGCFGSDILAGMDRAISDGVDVLSLSLGGGSAPYYRDTIAIGAFA 307

Query: 312 AMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKRFTGVSLYS 371
           AMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKRFTGVSLYS
Sbjct: 308 AMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKRFTGVSLYS 367

Query: 372 GQGMGSKPVELVYNKGSNTSSNMCLPGSLEPSVVRGKVVVCDRGINARVEKGGVVRDAGG 431
           GQGMG+K V LVYNKGSNTSSNMCLPGSL+P+VVRGKVVVCDRGINARVEKGGVVRDAGG
Sbjct: 368 GQGMGNKAVALVYNKGSNTSSNMCLPGSLDPAVVRGKVVVCDRGINARVEKGGVVRDAGG 427

Query: 432 IGMILANTAASGEELVADSHLLPAVAVGRKTGDLIRQYVRSISNPTAVLSFGGTILNVRP 491
           IGMILANTAASGEELVADSHLLPAVAVGRKTGDLIRQYVRS SNPTAVLSFGGTILNVRP
Sbjct: 428 IGMILANTAASGEELVADSHLLPAVAVGRKTGDLIRQYVRSDSNPTAVLSFGGTILNVRP 487

Query: 492 SPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLDNDKRKTQFNIMSGTS 551
           SPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGL+NDKRKTQFNIMSGTS
Sbjct: 488 SPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLENDKRKTQFNIMSGTS 547

Query: 552 MSCPHISGLAALLKAAHPQWSPSAIKSALMTTAYTQDNTNSSLRDAAGGGFSNPWAHGAG 611
           MSCPHISGLAALLKAAHPQWSPSAIKSALMTTAYTQDNTNSSLRDAAGGGFSNPWAHGAG
Sbjct: 548 MSCPHISGLAALLKAAHPQWSPSAIKSALMTTAYTQDNTNSSLRDAAGGGFSNPWAHGAG 607

Query: 612 HVDPHKALSPGLLYDISTDDYIAFLCSLDYGIDHVQAIVKRSNITCSRKFADPGQLNYPS 671
           HVDPHKALSPGLLYDIST+DYIAFLCSLDYGIDHVQAIVKRSNITCSRKFADPGQLNYPS
Sbjct: 608 HVDPHKALSPGLLYDISTNDYIAFLCSLDYGIDHVQAIVKRSNITCSRKFADPGQLNYPS 667

Query: 672 FSVVFGSKRVVRYTRIVTNVGAAGSVYEVATTAPSVVNVTVKPSKLVFTKVGERKRYTVT 731
           FSVVFGSKRVVRYTRIVTNVGAAGSVY+VATTAPSVV VTVKPSKLVFTKVGERKRYTVT
Sbjct: 668 FSVVFGSKRVVRYTRIVTNVGAAGSVYDVATTAPSVVKVTVKPSKLVFTKVGERKRYTVT 727

Query: 732 FVASRDAAQTTRFGFGSIEWSNDQHQVRSPVAFAWTRL 770
           FVASRDAAQTTRFGFGSI WSNDQHQVRSPV+FAWTRL
Sbjct: 728 FVASRDAAQTTRFGFGSIVWSNDQHQVRSPVSFAWTRL 765

BLAST of Lsi06G003400 vs. ExPASy TrEMBL
Match: A0A1S3BQE8 (subtilisin-like protease SBT1.8 OS=Cucumis melo OX=3656 GN=LOC103492374 PE=3 SV=1)

HSP 1 Score: 1440.2 bits (3727), Expect = 0.0e+00
Identity = 723/758 (95.38%), Postives = 740/758 (97.63%), Query Frame = 0

Query: 12  LLLLLLLLLLLPCVFVNAKKTYIVRMKHNALPSQYLTHHDWYSAHLQSLSSSSPSDSLLY 71
           L+  LLLLLLL CVF+NAKKTYIV MKH+ALPS+YLTHHDWYSA LQSLSSSS SDSLLY
Sbjct: 8   LIPFLLLLLLLSCVFINAKKTYIVHMKHHALPSEYLTHHDWYSASLQSLSSSSSSDSLLY 67

Query: 72  TYTSAYHGFAASLDSDEAELLRQSDSVLGVYEDTVYNLHTTRTPGFLGLDSDFGLWEGHN 131
           TYTS++HGFAA LDS+E ELLRQSDSVLGVYEDTVYNLHTTRTPGFLGLDSDFGLWEGH 
Sbjct: 68  TYTSSFHGFAAFLDSEEVELLRQSDSVLGVYEDTVYNLHTTRTPGFLGLDSDFGLWEGHT 127

Query: 132 TQDLNQASHDVIIGVLDTGIWPESKSFDDAGMPEIPSRWRGECESGPDFNPSLCNKKLIG 191
           TQDLNQASHDVIIGVLDTGIWPESKSFDD GMPEIPSRWRG CE+GPDF+PSLCNKKLIG
Sbjct: 128 TQDLNQASHDVIIGVLDTGIWPESKSFDDTGMPEIPSRWRGGCEAGPDFSPSLCNKKLIG 187

Query: 192 ARSFSKGYQMASGGGYFRKPRENESPRDQDGHGTHTASTAAGSHVANASLLGYARGIARG 251
           ARSFSKGYQMASGGGYFRKPREN+S RDQDGHGTHTASTAAGSHV NASLLGYARGIARG
Sbjct: 188 ARSFSKGYQMASGGGYFRKPRENQSARDQDGHGTHTASTAAGSHVPNASLLGYARGIARG 247

Query: 252 MAPQARVAAYKTCWPTGCFGSDILAGMDRAISDGVDVLSLSLGGGSAPYYRDTIAIGAFA 311
           MAPQARVAAYKTCWPTGCFGSDILAGMDRAISDGVDVLSLSLGGGSAPYYRDTIAIGAFA
Sbjct: 248 MAPQARVAAYKTCWPTGCFGSDILAGMDRAISDGVDVLSLSLGGGSAPYYRDTIAIGAFA 307

Query: 312 AMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKRFTGVSLYS 371
           AMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKRFTGVSLYS
Sbjct: 308 AMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKRFTGVSLYS 367

Query: 372 GQGMGSKPVELVYNKGSNTSSNMCLPGSLEPSVVRGKVVVCDRGINARVEKGGVVRDAGG 431
           GQGMG+K V LVYNKGSNTSSNMCLPGSL+P+VVRGKVVVCDRGINARVEKGGVVRDAGG
Sbjct: 368 GQGMGNKAVALVYNKGSNTSSNMCLPGSLDPAVVRGKVVVCDRGINARVEKGGVVRDAGG 427

Query: 432 IGMILANTAASGEELVADSHLLPAVAVGRKTGDLIRQYVRSISNPTAVLSFGGTILNVRP 491
           IGMILANTAASGEELVADSHLLPAVAVGRKTGDLIRQYVRS SNPTAVLSFGGTILNVRP
Sbjct: 428 IGMILANTAASGEELVADSHLLPAVAVGRKTGDLIRQYVRSDSNPTAVLSFGGTILNVRP 487

Query: 492 SPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLDNDKRKTQFNIMSGTS 551
           SPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGL+NDKRKTQFNIMSGTS
Sbjct: 488 SPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLENDKRKTQFNIMSGTS 547

Query: 552 MSCPHISGLAALLKAAHPQWSPSAIKSALMTTAYTQDNTNSSLRDAAGGGFSNPWAHGAG 611
           MSCPHISGLAALLKAAHPQWSPSAIKSALMTTAYTQDNTNSSLRDAAGGGFSNPWAHGAG
Sbjct: 548 MSCPHISGLAALLKAAHPQWSPSAIKSALMTTAYTQDNTNSSLRDAAGGGFSNPWAHGAG 607

Query: 612 HVDPHKALSPGLLYDISTDDYIAFLCSLDYGIDHVQAIVKRSNITCSRKFADPGQLNYPS 671
           HVDPHKALSPGLLYDIST+DYIAFLCSLDYGIDHVQAIVKRSNITCSRKFADPGQLNYPS
Sbjct: 608 HVDPHKALSPGLLYDISTNDYIAFLCSLDYGIDHVQAIVKRSNITCSRKFADPGQLNYPS 667

Query: 672 FSVVFGSKRVVRYTRIVTNVGAAGSVYEVATTAPSVVNVTVKPSKLVFTKVGERKRYTVT 731
           FSVVFGSKRVVRYTRIVTNVGAAGSVY+VATTAPSVV VTVKPSKLVFTKVGERKRYTVT
Sbjct: 668 FSVVFGSKRVVRYTRIVTNVGAAGSVYDVATTAPSVVKVTVKPSKLVFTKVGERKRYTVT 727

Query: 732 FVASRDAAQTTRFGFGSIEWSNDQHQVRSPVAFAWTRL 770
           FVASRDAAQTTRFGFGSI WSNDQHQVRSPV+FAWTRL
Sbjct: 728 FVASRDAAQTTRFGFGSIVWSNDQHQVRSPVSFAWTRL 765

BLAST of Lsi06G003400 vs. ExPASy TrEMBL
Match: A0A5D3CDV9 (Subtilisin-like protease SBT1.8 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold16G001530 PE=3 SV=1)

HSP 1 Score: 1438.7 bits (3723), Expect = 0.0e+00
Identity = 721/755 (95.50%), Postives = 738/755 (97.75%), Query Frame = 0

Query: 13  LLLLLLLLLLPCVFVNAKKTYIVRMKHNALPSQYLTHHDWYSAHLQSLSSSSPSDSLLYT 72
           L+  LLLLLL CVF+NAKKTYIV MKH+ALPS+YLTHHDWYSA LQSLSSSS SDSLLYT
Sbjct: 8   LIPFLLLLLLSCVFINAKKTYIVHMKHHALPSEYLTHHDWYSASLQSLSSSSSSDSLLYT 67

Query: 73  YTSAYHGFAASLDSDEAELLRQSDSVLGVYEDTVYNLHTTRTPGFLGLDSDFGLWEGHNT 132
           YTS++HGFAA LDS+E ELLRQSDSVLGVYEDTVYNLHTTRTPGFLGLDSDFGLWEGH T
Sbjct: 68  YTSSFHGFAAFLDSEEVELLRQSDSVLGVYEDTVYNLHTTRTPGFLGLDSDFGLWEGHTT 127

Query: 133 QDLNQASHDVIIGVLDTGIWPESKSFDDAGMPEIPSRWRGECESGPDFNPSLCNKKLIGA 192
           QDLNQASHDVIIGVLDTGIWPESKSFDD GMPEIPSRWRGECE+GPDF+PSLCNKKLIGA
Sbjct: 128 QDLNQASHDVIIGVLDTGIWPESKSFDDTGMPEIPSRWRGECEAGPDFSPSLCNKKLIGA 187

Query: 193 RSFSKGYQMASGGGYFRKPRENESPRDQDGHGTHTASTAAGSHVANASLLGYARGIARGM 252
           RSFSKGYQMASGGGYFRKPREN+S RDQDGHGTHTASTAAGSHV NASLLGYARGIARGM
Sbjct: 188 RSFSKGYQMASGGGYFRKPRENQSARDQDGHGTHTASTAAGSHVPNASLLGYARGIARGM 247

Query: 253 APQARVAAYKTCWPTGCFGSDILAGMDRAISDGVDVLSLSLGGGSAPYYRDTIAIGAFAA 312
           APQARVAAYKTCWPTGCFGSDILAGMDRAISDGVDVLSLSLGGGSAPYYRDTIAIGAFAA
Sbjct: 248 APQARVAAYKTCWPTGCFGSDILAGMDRAISDGVDVLSLSLGGGSAPYYRDTIAIGAFAA 307

Query: 313 MEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKRFTGVSLYSG 372
           MEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKRFTGVSLYSG
Sbjct: 308 MEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQLGNGKRFTGVSLYSG 367

Query: 373 QGMGSKPVELVYNKGSNTSSNMCLPGSLEPSVVRGKVVVCDRGINARVEKGGVVRDAGGI 432
           QGMG+K V LVYNKGSNTSSNMCLPGSL+P+VVRGKVVVCDRGINARVEKGGVVRDAGGI
Sbjct: 368 QGMGNKAVALVYNKGSNTSSNMCLPGSLDPAVVRGKVVVCDRGINARVEKGGVVRDAGGI 427

Query: 433 GMILANTAASGEELVADSHLLPAVAVGRKTGDLIRQYVRSISNPTAVLSFGGTILNVRPS 492
           GMILANTAASGEELVADSHLLPAVAVGRKTGDLIRQYVRS SNPTAVLSFGGTILNVRPS
Sbjct: 428 GMILANTAASGEELVADSHLLPAVAVGRKTGDLIRQYVRSDSNPTAVLSFGGTILNVRPS 487

Query: 493 PVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLDNDKRKTQFNIMSGTSM 552
           PVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGL+NDKRKTQFNIMSGTSM
Sbjct: 488 PVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLENDKRKTQFNIMSGTSM 547

Query: 553 SCPHISGLAALLKAAHPQWSPSAIKSALMTTAYTQDNTNSSLRDAAGGGFSNPWAHGAGH 612
           SCPHISGLAALLKAAHPQWSPSAIKSALMTTAYTQDNTNSSLRDAAGGGFSNPWAHGAGH
Sbjct: 548 SCPHISGLAALLKAAHPQWSPSAIKSALMTTAYTQDNTNSSLRDAAGGGFSNPWAHGAGH 607

Query: 613 VDPHKALSPGLLYDISTDDYIAFLCSLDYGIDHVQAIVKRSNITCSRKFADPGQLNYPSF 672
           VDPHKALSPGLLYDIST+DYIAFLCSLDYGIDHVQAIVKRSNITCSRKFADPGQLNYPSF
Sbjct: 608 VDPHKALSPGLLYDISTNDYIAFLCSLDYGIDHVQAIVKRSNITCSRKFADPGQLNYPSF 667

Query: 673 SVVFGSKRVVRYTRIVTNVGAAGSVYEVATTAPSVVNVTVKPSKLVFTKVGERKRYTVTF 732
           SVVFGSKRVVRYTRIVTNVGAAGSVY+VATTAPSVV VTVKPSKLVFTKVGERKRYTVTF
Sbjct: 668 SVVFGSKRVVRYTRIVTNVGAAGSVYDVATTAPSVVKVTVKPSKLVFTKVGERKRYTVTF 727

Query: 733 VASRDAAQTTRFGFGSIEWSNDQHQVRSPVAFAWT 768
           VASRDAAQTTRFGFGSI WSNDQHQVRSPV+FAWT
Sbjct: 728 VASRDAAQTTRFGFGSIVWSNDQHQVRSPVSFAWT 762

BLAST of Lsi06G003400 vs. NCBI nr
Match: XP_038879224.1 (subtilisin-like protease SBT1.8 [Benincasa hispida])

HSP 1 Score: 1466.4 bits (3795), Expect = 0.0e+00
Identity = 741/769 (96.36%), Postives = 750/769 (97.53%), Query Frame = 0

Query: 1   MGSMASHFLLLLLLLLLLLLLLPCVFVNAKKTYIVRMKHNALPSQYLTHHDWYSAHLQSL 60
           MGSMA       LL+  LLLLLPCVFVNAKKTYIVRMKHNALPS+YLTHHDWYSAHLQSL
Sbjct: 1   MGSMAR------LLIAFLLLLLPCVFVNAKKTYIVRMKHNALPSEYLTHHDWYSAHLQSL 60

Query: 61  SSSSPSDSLLYTYTSAYHGFAASLDSDEAELLRQSDSVLGVYEDTVYNLHTTRTPGFLGL 120
           SSSS SDSLLYTYTSAYHGFAASLDS+EAELLRQSDSVLGVYED+VY LHTTRTPGFLGL
Sbjct: 61  SSSSTSDSLLYTYTSAYHGFAASLDSNEAELLRQSDSVLGVYEDSVYTLHTTRTPGFLGL 120

Query: 121 DSDFGLWEGHNTQDLNQASHDVIIGVLDTGIWPESKSFDDAGMPEIPSRWRGECESGPDF 180
           DSDFGLWEGHNTQDLNQASHDVIIGVLDTGIWPESKSFDD GMPEIPSRWRGECESG DF
Sbjct: 121 DSDFGLWEGHNTQDLNQASHDVIIGVLDTGIWPESKSFDDTGMPEIPSRWRGECESGADF 180

Query: 181 NPSLCNKKLIGARSFSKGYQMASGGGYFRKPRENESPRDQDGHGTHTASTAAGSHVANAS 240
           +PSLCNKKLIGARSFSKGYQMASGGGYFRKPRENESPRDQDGHGTHTASTAAGSHVANAS
Sbjct: 181 SPSLCNKKLIGARSFSKGYQMASGGGYFRKPRENESPRDQDGHGTHTASTAAGSHVANAS 240

Query: 241 LLGYARGIARGMAPQARVAAYKTCWPTGCFGSDILAGMDRAISDGVDVLSLSLGGGSAPY 300
           LLGYARGIARGMAPQARVAAYKTCWPTGCFGSDILAGMDRAISDGVDVLSLSLGGGSAPY
Sbjct: 241 LLGYARGIARGMAPQARVAAYKTCWPTGCFGSDILAGMDRAISDGVDVLSLSLGGGSAPY 300

Query: 301 YRDTIAIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQLGN 360
           YRDTIAIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQ+GN
Sbjct: 301 YRDTIAIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQIGN 360

Query: 361 GKRFTGVSLYSGQGMGSKPVELVYNKGSNTSSNMCLPGSLEPSVVRGKVVVCDRGINARV 420
           GKRFTGVSLYSGQGMGSK VELVYNKGSNTSSNMCLPGSLEP+VVRGKVVVCDRGINARV
Sbjct: 361 GKRFTGVSLYSGQGMGSKAVELVYNKGSNTSSNMCLPGSLEPAVVRGKVVVCDRGINARV 420

Query: 421 EKGGVVRDAGGIGMILANTAASGEELVADSHLLPAVAVGRKTGDLIRQYVRSISNPTAVL 480
           EKGGVVRDAGGIGMILANTAASGEELVADSHLLPAVAVGRKTGDLIRQYVRS SNPTAVL
Sbjct: 421 EKGGVVRDAGGIGMILANTAASGEELVADSHLLPAVAVGRKTGDLIRQYVRSDSNPTAVL 480

Query: 481 SFGGTILNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLDNDKR 540
           SFGGTILNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLDNDKR
Sbjct: 481 SFGGTILNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLDNDKR 540

Query: 541 KTQFNIMSGTSMSCPHISGLAALLKAAHPQWSPSAIKSALMTTAYTQDNTNSSLRDAAGG 600
           KTQFNIMSGTSMSCPHISGLAALLKAAHPQWSPSAIKSALMTTAYTQDNTNSSLRDAAGG
Sbjct: 541 KTQFNIMSGTSMSCPHISGLAALLKAAHPQWSPSAIKSALMTTAYTQDNTNSSLRDAAGG 600

Query: 601 GFSNPWAHGAGHVDPHKALSPGLLYDISTDDYIAFLCSLDYGIDHVQAIVKRSNITCSRK 660
           GFSNPWAHGAGHVDPHKALSPGLLYDIST+DYIAFLCSLDYGIDHVQAIVKRSNITCSRK
Sbjct: 601 GFSNPWAHGAGHVDPHKALSPGLLYDISTNDYIAFLCSLDYGIDHVQAIVKRSNITCSRK 660

Query: 661 FADPGQLNYPSFSVVFGSKRVVRYTRIVTNVGAAGSVYEVATTAPSVVNVTVKPSKLVFT 720
           FADPGQLNYPSFSVVFGSKRVVRYTRIVTNVGAAGSVYEVATTAPSVV VTVKPSKLVFT
Sbjct: 661 FADPGQLNYPSFSVVFGSKRVVRYTRIVTNVGAAGSVYEVATTAPSVVKVTVKPSKLVFT 720

Query: 721 KVGERKRYTVTFVASRDAAQTTRFGFGSIEWSNDQHQVRSPVAFAWTRL 770
           KVGERKRYTVTFVASRDAAQT RFGFGSI W+N QHQVRSPVAFAWTRL
Sbjct: 721 KVGERKRYTVTFVASRDAAQTMRFGFGSIAWNNYQHQVRSPVAFAWTRL 763

BLAST of Lsi06G003400 vs. NCBI nr
Match: XP_023516159.1 (subtilisin-like protease SBT1.8 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1448.7 bits (3749), Expect = 0.0e+00
Identity = 727/769 (94.54%), Postives = 747/769 (97.14%), Query Frame = 0

Query: 1   MGSMASHFLLLLLLLLLLLLLLPCVFVNAKKTYIVRMKHNALPSQYLTHHDWYSAHLQSL 60
           MGSMAS    LL+   LLLLLLPCVFVNAK+TYIVRMKH+ALPS YLTHHDWYSAHLQSL
Sbjct: 1   MGSMAS----LLVAYFLLLLLLPCVFVNAKRTYIVRMKHHALPSDYLTHHDWYSAHLQSL 60

Query: 61  SSSSPSDSLLYTYTSAYHGFAASLDSDEAELLRQSDSVLGVYEDTVYNLHTTRTPGFLGL 120
           SSS+ SDSLLYTYTSAYHGFAASLD DEAELLRQSDSVLGVYEDTVYNLHTTRTPGFLGL
Sbjct: 61  SSSATSDSLLYTYTSAYHGFAASLDPDEAELLRQSDSVLGVYEDTVYNLHTTRTPGFLGL 120

Query: 121 DSDFGLWEGHNTQDLNQASHDVIIGVLDTGIWPESKSFDDAGMPEIPSRWRGECESGPDF 180
           DSDFGLWEGHNTQDL+QASHDVIIGVLDTGIWPESKSFDD GMPEIPSRWRGECESGPDF
Sbjct: 121 DSDFGLWEGHNTQDLDQASHDVIIGVLDTGIWPESKSFDDTGMPEIPSRWRGECESGPDF 180

Query: 181 NPSLCNKKLIGARSFSKGYQMASGGGYFRKPRENESPRDQDGHGTHTASTAAGSHVANAS 240
           +PSLCNKKLIGARSFSKGYQMASGGGYFRK RENESPRDQDGHGTHTASTAAGSHVANAS
Sbjct: 181 SPSLCNKKLIGARSFSKGYQMASGGGYFRKSRENESPRDQDGHGTHTASTAAGSHVANAS 240

Query: 241 LLGYARGIARGMAPQARVAAYKTCWPTGCFGSDILAGMDRAISDGVDVLSLSLGGGSAPY 300
           LLGYARGIARGMAPQARVAAYKTCWPTGCFGSDILAGMDRAISDGVDVLSLSLGGGSAPY
Sbjct: 241 LLGYARGIARGMAPQARVAAYKTCWPTGCFGSDILAGMDRAISDGVDVLSLSLGGGSAPY 300

Query: 301 YRDTIAIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQLGN 360
           YRDTIAIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQLGN
Sbjct: 301 YRDTIAIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQLGN 360

Query: 361 GKRFTGVSLYSGQGMGSKPVELVYNKGSNTSSNMCLPGSLEPSVVRGKVVVCDRGINARV 420
           GKRFTGVSLYSGQGMG+KPV LVYNKGSN+SSNMCLPGSLEP+ VRGKVV+CDRGINARV
Sbjct: 361 GKRFTGVSLYSGQGMGNKPVALVYNKGSNSSSNMCLPGSLEPAFVRGKVVICDRGINARV 420

Query: 421 EKGGVVRDAGGIGMILANTAASGEELVADSHLLPAVAVGRKTGDLIRQYVRSISNPTAVL 480
           EKGGVVRDAGGIGMILANTAASGEELVADSHLLPAVAVG KTGDLIRQYVRS++ PTAVL
Sbjct: 421 EKGGVVRDAGGIGMILANTAASGEELVADSHLLPAVAVGAKTGDLIRQYVRSVAKPTAVL 480

Query: 481 SFGGTILNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLDNDKR 540
           SFGGT+LNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLDNDKR
Sbjct: 481 SFGGTVLNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLDNDKR 540

Query: 541 KTQFNIMSGTSMSCPHISGLAALLKAAHPQWSPSAIKSALMTTAYTQDNTNSSLRDAAGG 600
           KTQFNIMSGTSMSCPHISGLAALLKAAHP WSPSAIKSALMTTAYTQDNTNSSLRDAAGG
Sbjct: 541 KTQFNIMSGTSMSCPHISGLAALLKAAHPSWSPSAIKSALMTTAYTQDNTNSSLRDAAGG 600

Query: 601 GFSNPWAHGAGHVDPHKALSPGLLYDISTDDYIAFLCSLDYGIDHVQAIVKRSNITCSRK 660
            FSNPWAHGAGHVDPHKALSPGL+YDIST+DYI FLCSLDYGIDHVQAI KRSNITCS+K
Sbjct: 601 MFSNPWAHGAGHVDPHKALSPGLVYDISTNDYITFLCSLDYGIDHVQAIAKRSNITCSKK 660

Query: 661 FADPGQLNYPSFSVVFGSKRVVRYTRIVTNVGAAGSVYEVATTAPSVVNVTVKPSKLVFT 720
           FADPGQLNYPSFSVVFGSKRVVRYTRIVTNVGAAGSVYEVATTAPSVV VTVKPSKLVF+
Sbjct: 661 FADPGQLNYPSFSVVFGSKRVVRYTRIVTNVGAAGSVYEVATTAPSVVKVTVKPSKLVFS 720

Query: 721 KVGERKRYTVTFVASRDAAQTTRFGFGSIEWSNDQHQVRSPVAFAWTRL 770
           KVGERKRYTVTFVASRDAA+TTR+GFGSI WSNDQHQVRSPVAFAWT+L
Sbjct: 721 KVGERKRYTVTFVASRDAARTTRYGFGSIAWSNDQHQVRSPVAFAWTKL 765

BLAST of Lsi06G003400 vs. NCBI nr
Match: XP_022987083.1 (subtilisin-like protease SBT1.8 [Cucurbita maxima])

HSP 1 Score: 1447.6 bits (3746), Expect = 0.0e+00
Identity = 728/769 (94.67%), Postives = 747/769 (97.14%), Query Frame = 0

Query: 1   MGSMASHFLLLLLLLLLLLLLLPCVFVNAKKTYIVRMKHNALPSQYLTHHDWYSAHLQSL 60
           MGSMA    LL+   LLLLLLLPCVFVNAK+TYIVRMKH+ALPS YLTHHDWYSAHLQSL
Sbjct: 1   MGSMAR---LLVASFLLLLLLLPCVFVNAKRTYIVRMKHHALPSDYLTHHDWYSAHLQSL 60

Query: 61  SSSSPSDSLLYTYTSAYHGFAASLDSDEAELLRQSDSVLGVYEDTVYNLHTTRTPGFLGL 120
           SSS+ SDSLLYTYTSAYHGFAASLD DEAELLRQSDSVLGVYEDTVY LHTTRTPGFLGL
Sbjct: 61  SSSATSDSLLYTYTSAYHGFAASLDPDEAELLRQSDSVLGVYEDTVYKLHTTRTPGFLGL 120

Query: 121 DSDFGLWEGHNTQDLNQASHDVIIGVLDTGIWPESKSFDDAGMPEIPSRWRGECESGPDF 180
           DSDFGLWEGHNTQDL+QASHDVIIGVLDTGIWPESKSFDD GMPEIPSRWRGECESGPDF
Sbjct: 121 DSDFGLWEGHNTQDLDQASHDVIIGVLDTGIWPESKSFDDTGMPEIPSRWRGECESGPDF 180

Query: 181 NPSLCNKKLIGARSFSKGYQMASGGGYFRKPRENESPRDQDGHGTHTASTAAGSHVANAS 240
           +PSLCNKKLIGARSFSKGYQMASGGGYFRK RENESPRDQDGHGTHTASTAAGSHVANAS
Sbjct: 181 SPSLCNKKLIGARSFSKGYQMASGGGYFRKSRENESPRDQDGHGTHTASTAAGSHVANAS 240

Query: 241 LLGYARGIARGMAPQARVAAYKTCWPTGCFGSDILAGMDRAISDGVDVLSLSLGGGSAPY 300
           LLGYARGIARGMAPQARVAAYKTCWPTGCFGSDILAGMDRAISDGVDVLSLSLGGGSAPY
Sbjct: 241 LLGYARGIARGMAPQARVAAYKTCWPTGCFGSDILAGMDRAISDGVDVLSLSLGGGSAPY 300

Query: 301 YRDTIAIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQLGN 360
           YRDTIAIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQLGN
Sbjct: 301 YRDTIAIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQLGN 360

Query: 361 GKRFTGVSLYSGQGMGSKPVELVYNKGSNTSSNMCLPGSLEPSVVRGKVVVCDRGINARV 420
           GKRFTGVSLYSGQGMG+KPV LVYNKGSN+SSNMCLPGSLEP+ VRGKVV+CDRGINARV
Sbjct: 361 GKRFTGVSLYSGQGMGNKPVALVYNKGSNSSSNMCLPGSLEPAFVRGKVVICDRGINARV 420

Query: 421 EKGGVVRDAGGIGMILANTAASGEELVADSHLLPAVAVGRKTGDLIRQYVRSISNPTAVL 480
           EKGGVVRDAGGIGMILANTAASGEELVADSHLLPAVAVG KTGDLIRQYVRS++ PTAVL
Sbjct: 421 EKGGVVRDAGGIGMILANTAASGEELVADSHLLPAVAVGAKTGDLIRQYVRSVAKPTAVL 480

Query: 481 SFGGTILNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLDNDKR 540
           SFGGT+LNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLDNDKR
Sbjct: 481 SFGGTVLNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLDNDKR 540

Query: 541 KTQFNIMSGTSMSCPHISGLAALLKAAHPQWSPSAIKSALMTTAYTQDNTNSSLRDAAGG 600
           KTQFNIMSGTSMSCPHISGLAALLKAAHP WSPSAIKSALMTTAYTQDNTNSSLRDAAGG
Sbjct: 541 KTQFNIMSGTSMSCPHISGLAALLKAAHPSWSPSAIKSALMTTAYTQDNTNSSLRDAAGG 600

Query: 601 GFSNPWAHGAGHVDPHKALSPGLLYDISTDDYIAFLCSLDYGIDHVQAIVKRSNITCSRK 660
            FSNPWAHGAGHVDPHKALSPGLLYDIST+DYI FLCSLDYGIDHVQAIVKRSNITCS+K
Sbjct: 601 MFSNPWAHGAGHVDPHKALSPGLLYDISTNDYITFLCSLDYGIDHVQAIVKRSNITCSKK 660

Query: 661 FADPGQLNYPSFSVVFGSKRVVRYTRIVTNVGAAGSVYEVATTAPSVVNVTVKPSKLVFT 720
           FADPGQLNYPSFSVVFGSKRVVRYTRIVTNVGAAGSVYEVATTAPSVV VTVKPSKLVF+
Sbjct: 661 FADPGQLNYPSFSVVFGSKRVVRYTRIVTNVGAAGSVYEVATTAPSVVKVTVKPSKLVFS 720

Query: 721 KVGERKRYTVTFVASRDAAQTTRFGFGSIEWSNDQHQVRSPVAFAWTRL 770
           KVGERKRYTVTFVASRDAA+TTR+GFGSI WSNDQHQVRSPVAFAWT+L
Sbjct: 721 KVGERKRYTVTFVASRDAARTTRYGFGSIAWSNDQHQVRSPVAFAWTKL 766

BLAST of Lsi06G003400 vs. NCBI nr
Match: XP_022960792.1 (subtilisin-like protease SBT1.8 [Cucurbita moschata] >KAG7023704.1 Subtilisin-like protease SBT1.8 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1446.0 bits (3742), Expect = 0.0e+00
Identity = 726/769 (94.41%), Postives = 746/769 (97.01%), Query Frame = 0

Query: 1   MGSMASHFLLLLLLLLLLLLLLPCVFVNAKKTYIVRMKHNALPSQYLTHHDWYSAHLQSL 60
           MGSMA    LL+   LLLLLLLPCVFVNAK+TYIVRMKH+ALPS YLTHHDWYSAHLQSL
Sbjct: 1   MGSMAR---LLVASFLLLLLLLPCVFVNAKRTYIVRMKHHALPSDYLTHHDWYSAHLQSL 60

Query: 61  SSSSPSDSLLYTYTSAYHGFAASLDSDEAELLRQSDSVLGVYEDTVYNLHTTRTPGFLGL 120
           SSS+ SDSLLYTYTSAYHGFAASLD DEAELLRQSDSVLGVYEDTVYNLHTTRTPGFLGL
Sbjct: 61  SSSATSDSLLYTYTSAYHGFAASLDPDEAELLRQSDSVLGVYEDTVYNLHTTRTPGFLGL 120

Query: 121 DSDFGLWEGHNTQDLNQASHDVIIGVLDTGIWPESKSFDDAGMPEIPSRWRGECESGPDF 180
           DSDFGLWEGHNTQDL+QASHDVIIGVLDTGIWPESKSFDD GMPEIPSRWRGECESGPDF
Sbjct: 121 DSDFGLWEGHNTQDLDQASHDVIIGVLDTGIWPESKSFDDTGMPEIPSRWRGECESGPDF 180

Query: 181 NPSLCNKKLIGARSFSKGYQMASGGGYFRKPRENESPRDQDGHGTHTASTAAGSHVANAS 240
           +PSLCNKKLIGARSFSKGYQMASGGGYFRK RENESPRDQDGHGTHTASTAAGSHVANAS
Sbjct: 181 SPSLCNKKLIGARSFSKGYQMASGGGYFRKSRENESPRDQDGHGTHTASTAAGSHVANAS 240

Query: 241 LLGYARGIARGMAPQARVAAYKTCWPTGCFGSDILAGMDRAISDGVDVLSLSLGGGSAPY 300
           LLGYARGIARGMAPQARVAAYKTCWPTGCFGSDILAGMDRAISDGVDVLSLSLGGGSAPY
Sbjct: 241 LLGYARGIARGMAPQARVAAYKTCWPTGCFGSDILAGMDRAISDGVDVLSLSLGGGSAPY 300

Query: 301 YRDTIAIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQLGN 360
           YRDTIAIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQLGN
Sbjct: 301 YRDTIAIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQLGN 360

Query: 361 GKRFTGVSLYSGQGMGSKPVELVYNKGSNTSSNMCLPGSLEPSVVRGKVVVCDRGINARV 420
           GKRFTGVSLYSGQGMG+KPV LVYNKGSN+SSNMCLPGSLEP+ VRGKVV+CDRGINARV
Sbjct: 361 GKRFTGVSLYSGQGMGNKPVALVYNKGSNSSSNMCLPGSLEPAFVRGKVVICDRGINARV 420

Query: 421 EKGGVVRDAGGIGMILANTAASGEELVADSHLLPAVAVGRKTGDLIRQYVRSISNPTAVL 480
           EKGGVVRDAGGIGMILANTAASGEELVADSHLLPAVAVG KTGDLIRQYVRS++ PTAVL
Sbjct: 421 EKGGVVRDAGGIGMILANTAASGEELVADSHLLPAVAVGAKTGDLIRQYVRSVAKPTAVL 480

Query: 481 SFGGTILNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLDNDKR 540
           SFGGT+LNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLDNDKR
Sbjct: 481 SFGGTVLNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLDNDKR 540

Query: 541 KTQFNIMSGTSMSCPHISGLAALLKAAHPQWSPSAIKSALMTTAYTQDNTNSSLRDAAGG 600
           KTQFNIMSGTSMSCPHISGLAALLKAAHP WSPSAIKSALMTTAYTQDNTNSSLRDAAGG
Sbjct: 541 KTQFNIMSGTSMSCPHISGLAALLKAAHPSWSPSAIKSALMTTAYTQDNTNSSLRDAAGG 600

Query: 601 GFSNPWAHGAGHVDPHKALSPGLLYDISTDDYIAFLCSLDYGIDHVQAIVKRSNITCSRK 660
            FSNPWAHGAGHVDPHKALSPGL+YDIST+DYI FLCSLDYGIDHVQAI KRSNITC +K
Sbjct: 601 AFSNPWAHGAGHVDPHKALSPGLVYDISTNDYITFLCSLDYGIDHVQAIAKRSNITCLKK 660

Query: 661 FADPGQLNYPSFSVVFGSKRVVRYTRIVTNVGAAGSVYEVATTAPSVVNVTVKPSKLVFT 720
           FADPGQLNYPSFSVVFGSKRVVRYTRIVTNVGAAGSVYEVATTAPSVV VTVKPSKLVF+
Sbjct: 661 FADPGQLNYPSFSVVFGSKRVVRYTRIVTNVGAAGSVYEVATTAPSVVKVTVKPSKLVFS 720

Query: 721 KVGERKRYTVTFVASRDAAQTTRFGFGSIEWSNDQHQVRSPVAFAWTRL 770
           KVGERKRYTVTFVASRDAA+TTR+GFGSI WSNDQHQVRSPVAFAWT+L
Sbjct: 721 KVGERKRYTVTFVASRDAARTTRYGFGSIAWSNDQHQVRSPVAFAWTKL 766

BLAST of Lsi06G003400 vs. NCBI nr
Match: KAG6590040.1 (Subtilisin-like protease 1.8, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1445.3 bits (3740), Expect = 0.0e+00
Identity = 725/769 (94.28%), Postives = 745/769 (96.88%), Query Frame = 0

Query: 1   MGSMASHFLLLLLLLLLLLLLLPCVFVNAKKTYIVRMKHNALPSQYLTHHDWYSAHLQSL 60
           MGSMA     LL+   LLLLLLPCVFVNAK+TYIVRMKH+ALPS YLTHHDWYSAHLQSL
Sbjct: 1   MGSMAR----LLVASFLLLLLLPCVFVNAKRTYIVRMKHHALPSDYLTHHDWYSAHLQSL 60

Query: 61  SSSSPSDSLLYTYTSAYHGFAASLDSDEAELLRQSDSVLGVYEDTVYNLHTTRTPGFLGL 120
           SSS+ SDSLLYTYTSAYHGFAASLD DEAELLRQSDSVLGVYEDTVYNLHTTRTPGFLGL
Sbjct: 61  SSSATSDSLLYTYTSAYHGFAASLDPDEAELLRQSDSVLGVYEDTVYNLHTTRTPGFLGL 120

Query: 121 DSDFGLWEGHNTQDLNQASHDVIIGVLDTGIWPESKSFDDAGMPEIPSRWRGECESGPDF 180
           DSDFGLWEGHNTQDL+QASHDVIIGVLDTGIWPESKSFDD GMPEIPSRWRGECESGPDF
Sbjct: 121 DSDFGLWEGHNTQDLDQASHDVIIGVLDTGIWPESKSFDDTGMPEIPSRWRGECESGPDF 180

Query: 181 NPSLCNKKLIGARSFSKGYQMASGGGYFRKPRENESPRDQDGHGTHTASTAAGSHVANAS 240
           +PSLCNKKLIGARSFSKGYQMASGGGYFRK RENESPRDQDGHGTHTASTAAGSHVANAS
Sbjct: 181 SPSLCNKKLIGARSFSKGYQMASGGGYFRKSRENESPRDQDGHGTHTASTAAGSHVANAS 240

Query: 241 LLGYARGIARGMAPQARVAAYKTCWPTGCFGSDILAGMDRAISDGVDVLSLSLGGGSAPY 300
           LLGYARGIARGMAPQARVAAYKTCWPTGCFGSDILAGMDRAISDGVDVLSLSLGGGSAPY
Sbjct: 241 LLGYARGIARGMAPQARVAAYKTCWPTGCFGSDILAGMDRAISDGVDVLSLSLGGGSAPY 300

Query: 301 YRDTIAIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQLGN 360
           YRDTIAIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQLGN
Sbjct: 301 YRDTIAIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQLGN 360

Query: 361 GKRFTGVSLYSGQGMGSKPVELVYNKGSNTSSNMCLPGSLEPSVVRGKVVVCDRGINARV 420
           GKRFTGVSLYSGQGMG+KPV LVYNKGSN+SSNMCLPGSLEP+ VRGKVV+CDRGINARV
Sbjct: 361 GKRFTGVSLYSGQGMGNKPVALVYNKGSNSSSNMCLPGSLEPAFVRGKVVICDRGINARV 420

Query: 421 EKGGVVRDAGGIGMILANTAASGEELVADSHLLPAVAVGRKTGDLIRQYVRSISNPTAVL 480
           EKGGVVRDAGGIGMILANTAASGEELVADSHLLPAVAVG KTGDLIRQYVRS++ PTAVL
Sbjct: 421 EKGGVVRDAGGIGMILANTAASGEELVADSHLLPAVAVGAKTGDLIRQYVRSVAKPTAVL 480

Query: 481 SFGGTILNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLDNDKR 540
           SFGGT+LNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLDNDKR
Sbjct: 481 SFGGTVLNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLDNDKR 540

Query: 541 KTQFNIMSGTSMSCPHISGLAALLKAAHPQWSPSAIKSALMTTAYTQDNTNSSLRDAAGG 600
           KTQFNIMSGTSMSCPHISGLAALLKAAHP WSPSAIKSALMTTAYTQDNTNSSLRDAAGG
Sbjct: 541 KTQFNIMSGTSMSCPHISGLAALLKAAHPSWSPSAIKSALMTTAYTQDNTNSSLRDAAGG 600

Query: 601 GFSNPWAHGAGHVDPHKALSPGLLYDISTDDYIAFLCSLDYGIDHVQAIVKRSNITCSRK 660
            FSNPWAHGAGHVDPHKALSPGL+YDIST+DYI FLCSLDYGIDHVQAI KRSNITC +K
Sbjct: 601 AFSNPWAHGAGHVDPHKALSPGLVYDISTNDYITFLCSLDYGIDHVQAIAKRSNITCLKK 660

Query: 661 FADPGQLNYPSFSVVFGSKRVVRYTRIVTNVGAAGSVYEVATTAPSVVNVTVKPSKLVFT 720
           FADPGQLNYPSFSVVFGSKRVVRYTRIVTNVGAAGSVYEVATTAPSVV VTVKPSKLVF+
Sbjct: 661 FADPGQLNYPSFSVVFGSKRVVRYTRIVTNVGAAGSVYEVATTAPSVVKVTVKPSKLVFS 720

Query: 721 KVGERKRYTVTFVASRDAAQTTRFGFGSIEWSNDQHQVRSPVAFAWTRL 770
           KVGERKRYTVTFVASRDAA+TTR+GFGSI WSNDQHQVRSPVAFAWT+L
Sbjct: 721 KVGERKRYTVTFVASRDAARTTRYGFGSIAWSNDQHQVRSPVAFAWTKL 765

BLAST of Lsi06G003400 vs. TAIR 10
Match: AT2G05920.1 (Subtilase family protein )

HSP 1 Score: 1104.7 bits (2856), Expect = 0.0e+00
Identity = 553/769 (71.91%), Postives = 644/769 (83.75%), Query Frame = 0

Query: 1   MGSMASHFLLLLLLLLLLLLLLPCVFVNAKKTYIVRMKHNALPSQYLTHHDWYSAHLQSL 60
           M S +S    + ++   L LLL      AKKTYI+R+ H+  P  +LTHHDWY++ L S 
Sbjct: 1   MASSSSSSSSITIITTFLFLLL---HTTAKKTYIIRVNHSDKPESFLTHHDWYTSQLNSE 60

Query: 61  SSSSPSDSLLYTYTSAYHGFAASLDSDEAE-LLRQSDSVLGVYEDTVYNLHTTRTPGFLG 120
           S      SLLYTYT+++HGF+A LDS EA+ LL  S+S+L ++ED +Y LHTTRTP FLG
Sbjct: 61  S------SLLYTYTTSFHGFSAYLDSTEADSLLSSSNSILDIFEDPLYTLHTTRTPEFLG 120

Query: 121 LDSDFGLWEGHNTQDLNQASHDVIIGVLDTGIWPESKSFDDAGMPEIPSRWRGECESGPD 180
           L+S+FG+       DL  +S+ VIIGVLDTG+WPES+SFDD  MPEIPS+W+GECESG D
Sbjct: 121 LNSEFGV------HDLGSSSNGVIIGVLDTGVWPESRSFDDTDMPEIPSKWKGECESGSD 180

Query: 181 FNPSLCNKKLIGARSFSKGYQMASGGGYFRKPRENESPRDQDGHGTHTASTAAGSHVANA 240
           F+  LCNKKLIGARSFSKG+QMASGGG+  K RE+ SPRD DGHGTHT++TAAGS V NA
Sbjct: 181 FDSKLCNKKLIGARSFSKGFQMASGGGFSSK-RESVSPRDVDGHGTHTSTTAAGSAVRNA 240

Query: 241 SLLGYARGIARGMAPQARVAAYKTCWPTGCFGSDILAGMDRAISDGVDVLSLSLGGGSAP 300
           S LGYA G ARGMA +ARVA YK CW TGCFGSDILA MDRAI DGVDVLSLSLGGGSAP
Sbjct: 241 SFLGYAAGTARGMATRARVATYKVCWSTGCFGSDILAAMDRAILDGVDVLSLSLGGGSAP 300

Query: 301 YYRDTIAIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQLG 360
           YYRDTIAIGAF+AME+GVFVSCSAGNSGP +AS+ANVAPW+MTVGAGTLDRDFPA+  LG
Sbjct: 301 YYRDTIAIGAFSAMERGVFVSCSAGNSGPTRASVANVAPWVMTVGAGTLDRDFPAFANLG 360

Query: 361 NGKRFTGVSLYSGQGMGSKPVELVYNKGSNTSSNMCLPGSLEPSVVRGKVVVCDRGINAR 420
           NGKR TGVSLYSG GMG+KP+ELVYNKG+++SSN+CLPGSL+ S+VRGK+VVCDRG+NAR
Sbjct: 361 NGKRLTGVSLYSGVGMGTKPLELVYNKGNSSSSNLCLPGSLDSSIVRGKIVVCDRGVNAR 420

Query: 421 VEKGGVVRDAGGIGMILANTAASGEELVADSHLLPAVAVGRKTGDLIRQYVRSISNPTAV 480
           VEKG VVRDAGG+GMI+ANTAASGEELVADSHLLPA+AVG+KTGDL+R+YV+S S PTA+
Sbjct: 421 VEKGAVVRDAGGLGMIMANTAASGEELVADSHLLPAIAVGKKTGDLLREYVKSDSKPTAL 480

Query: 481 LSFGGTILNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLDNDK 540
           L F GT+L+V+PSPVVAAFSSRGPN VTP+ILKPDVIGPGVNILA WS++IGPTGLD D 
Sbjct: 481 LVFKGTVLDVKPSPVVAAFSSRGPNTVTPEILKPDVIGPGVNILAGWSDAIGPTGLDKDS 540

Query: 541 RKTQFNIMSGTSMSCPHISGLAALLKAAHPQWSPSAIKSALMTTAYTQDNTNSSLRDAAG 600
           R+TQFNIMSGTSMSCPHISGLA LLKAAHP+WSPSAIKSALMTTAY  DNTN+ L DAA 
Sbjct: 541 RRTQFNIMSGTSMSCPHISGLAGLLKAAHPEWSPSAIKSALMTTAYVLDNTNAPLHDAAD 600

Query: 601 GGFSNPWAHGAGHVDPHKALSPGLLYDISTDDYIAFLCSLDYGIDHVQAIVKRSNITCSR 660
              SNP+AHG+GHVDP KALSPGL+YDIST++YI FLCSLDY +DH+ AIVKR ++ CS+
Sbjct: 601 NSLSNPYAHGSGHVDPQKALSPGLVYDISTEEYIRFLCSLDYTVDHIVAIVKRPSVNCSK 660

Query: 661 KFADPGQLNYPSFSVVFGSKRVVRYTRIVTNVGAAGSVYEVATTAPSVVNVTVKPSKLVF 720
           KF+DPGQLNYPSFSV+FG KRVVRYTR VTNVGAA SVY+V       V ++VKPSKL F
Sbjct: 661 KFSDPGQLNYPSFSVLFGGKRVVRYTREVTNVGAASSVYKVTVNGAPSVGISVKPSKLSF 720

Query: 721 TKVGERKRYTVTFVASRDAAQTTRFGFGSIEWSNDQHQVRSPVAFAWTR 769
             VGE+KRYTVTFV+ +  + T +  FGSI WSN QH+VRSPVAF+W R
Sbjct: 721 KSVGEKKRYTVTFVSKKGVSMTNKAEFGSITWSNPQHEVRSPVAFSWNR 753

BLAST of Lsi06G003400 vs. TAIR 10
Match: AT5G67360.1 (Subtilase family protein )

HSP 1 Score: 803.5 bits (2074), Expect = 1.5e-232
Identity = 429/772 (55.57%), Postives = 543/772 (70.34%), Query Frame = 0

Query: 4   MASHFLLLLLLLLLLLLLLPCVFVNA--KKTYIVRMKHNALPSQYLTHHDWYSAHLQSLS 63
           M+S FL      LLL L    V  ++  + TYIV M  + +PS +  H +WY + L+S+S
Sbjct: 1   MSSSFLSSTAFFLLLCLGFCHVSSSSSDQGTYIVHMAKSQMPSSFDLHSNWYDSSLRSIS 60

Query: 64  SSSPSDSLLYTYTSAYHGFAASLDSDEAELLRQSDSVLGVYEDTVYNLHTTRTPGFLGLD 123
            S+    LLYTY +A HGF+  L  +EA+ L     V+ V  +  Y LHTTRTP FLGLD
Sbjct: 61  DSA---ELLYTYENAIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLD 120

Query: 124 SDFGLWEGHNTQDLNQAS---HDVIIGVLDTGIWPESKSFDDAGMPEIPSRWRGECESGP 183
                    +T DL   +    DV++GVLDTG+WPESKS+ D G   IPS W+G CE+G 
Sbjct: 121 --------EHTADLFPEAGSYSDVVVGVLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGT 180

Query: 184 DFNPSLCNKKLIGARSFSKGYQMASGGGYFRKPRENESPRDQDGHGTHTASTAAGSHVAN 243
           +F  SLCN+KLIGAR F++GY+  S  G   + +E+ SPRD DGHGTHT+STAAGS V  
Sbjct: 181 NFTASLCNRKLIGARFFARGYE--STMGPIDESKESRSPRDDDGHGTHTSSTAAGSVVEG 240

Query: 244 ASLLGYARGIARGMAPQARVAAYKTCWPTGCFGSDILAGMDRAISDGVDVLSLSLGGGSA 303
           ASLLGYA G ARGMAP+ARVA YK CW  GCF SDILA +D+AI+D V+VLS+SLGGG +
Sbjct: 241 ASLLGYASGTARGMAPRARVAVYKVCWLGGCFSSDILAAIDKAIADNVNVLSMSLGGGMS 300

Query: 304 PYYRDTIAIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQL 363
            YYRD +AIGAFAAME+G+ VSCSAGN+GP+ +SL+NVAPWI TVGAGTLDRDFPA   L
Sbjct: 301 DYYRDGVAIGAFAAMERGILVSCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAIL 360

Query: 364 GNGKRFTGVSLYSGQGMGSKPVELVY--NKGSNTSSNMCLPGSLEPSVVRGKVVVCDRGI 423
           GNGK FTGVSL+ G+ +  K +  +Y  N  + T+ N+C+ G+L P  V+GK+V+CDRGI
Sbjct: 361 GNGKNFTGVSLFKGEALPDKLLPFIYAGNASNATNGNLCMTGTLIPEKVKGKIVMCDRGI 420

Query: 424 NARVEKGGVVRDAGGIGMILANTAASGEELVADSHLLPAVAVGRKTGDLIRQYVRSISNP 483
           NARV+KG VV+ AGG+GMILANTAA+GEELVAD+HLLPA  VG K GD+IR YV +  NP
Sbjct: 421 NARVQKGDVVKAAGGVGMILANTAANGEELVADAHLLPATTVGEKAGDIIRHYVTTDPNP 480

Query: 484 TAVLSFGGTILNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLD 543
           TA +S  GT++ V+PSPVVAAFSSRGPN +TP ILKPD+I PGVNILAAW+ + GPTGL 
Sbjct: 481 TASISILGTVVGVKPSPVVAAFSSRGPNSITPNILKPDLIAPGVNILAAWTGAAGPTGLA 540

Query: 544 NDKRKTQFNIMSGTSMSCPHISGLAALLKAAHPQWSPSAIKSALMTTAYTQDNTNSSLRD 603
           +D R+ +FNI+SGTSMSCPH+SGLAALLK+ HP+WSP+AI+SALMTTAY        L D
Sbjct: 541 SDSRRVEFNIISGTSMSCPHVSGLAALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLD 600

Query: 604 AAGGGFSNPWAHGAGHVDPHKALSPGLLYDISTDDYIAFLCSLDYGIDHVQAIVKRSNIT 663
            A G  S P+ HGAGHV P  A +PGL+YD++T+DY+ FLC+L+Y    +++ V R N T
Sbjct: 601 IATGKPSTPFDHGAGHVSPTTATNPGLIYDLTTEDYLGFLCALNYTSPQIRS-VSRRNYT 660

Query: 664 CS-RKFADPGQLNYPSFSVVFGSKRVVRYTRIVTNVGAAGSVYEVATTAPSVVNVTVKPS 723
           C   K      LNYPSF+V        +YTR VT+VG AG+     T+  + V ++V+P+
Sbjct: 661 CDPSKSYSVADLNYPSFAVNVDGVGAYKYTRTVTSVGGAGTYSVKVTSETTGVKISVEPA 720

Query: 724 KLVFTKVGERKRYTVTFVASRDAAQTTRFGFGSIEWSNDQHQVRSPVAFAWT 768
            L F +  E+K YTVTF         +   FGSIEWS+ +H V SPVA +WT
Sbjct: 721 VLNFKEANEKKSYTVTFTVDSSKPSGSN-SFGSIEWSDGKHVVGSPVAISWT 757

BLAST of Lsi06G003400 vs. TAIR 10
Match: AT3G14067.1 (Subtilase family protein )

HSP 1 Score: 758.8 bits (1958), Expect = 4.1e-219
Identity = 413/774 (53.36%), Postives = 520/774 (67.18%), Query Frame = 0

Query: 10  LLLLLLLLLLLLLPCVF------VNAKKTYIVRMKHNALPSQYLTHHDWYSAHLQSLSSS 69
           L L  +  +  LL C F       +  ++YIV ++ +  PS + +H++W+ + L+SL SS
Sbjct: 4   LSLSSIFFVFPLLLCFFSPSSSSSDGLESYIVHVQRSHKPSLFSSHNNWHVSLLRSLPSS 63

Query: 70  SPSDSLLYTYTSAYHGFAASLDSDEAELLRQSDSVLGVYEDTVYNLHTTRTPGFLGLDSD 129
               +LLY+Y+ A HGF+A L   +   LR+  SV+ V  D    +HTT TP FLG   +
Sbjct: 64  PQPATLLYSYSRAVHGFSARLSPIQTAALRRHPSVISVIPDQAREIHTTHTPAFLGFSQN 123

Query: 130 FGLWEGHNTQDLNQASHDVIIGVLDTGIWPESKSFDDAGMPEIPSRWRGECESGPDFNPS 189
            GLW   N         DVI+GVLDTGIWPE  SF D+G+  IPS W+GECE GPDF  S
Sbjct: 124 SGLWSNSN------YGEDVIVGVLDTGIWPEHPSFSDSGLGPIPSTWKGECEIGPDFPAS 183

Query: 190 LCNKKLIGARSFSKGYQMASGGGYFRKPRENESPRDQDGHGTHTASTAAGSHVANASLLG 249
            CN+KLIGAR+F +GY     G      +E+ SPRD +GHGTHTASTAAGS VANASL  
Sbjct: 184 SCNRKLIGARAFYRGYLTQRNGTKKHAAKESRSPRDTEGHGTHTASTAAGSVVANASLYQ 243

Query: 250 YARGIARGMAPQARVAAYKTCWPTGCFGSDILAGMDRAISDGVDVLSLSLG-GGSAP-YY 309
           YARG A GMA +AR+AAYK CW  GC+ SDILA MD+A++DGV V+SLS+G  GSAP Y+
Sbjct: 244 YARGTATGMASKARIAAYKICWTGGCYDSDILAAMDQAVADGVHVISLSVGASGSAPEYH 303

Query: 310 RDTIAIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQLGNG 369
            D+IAIGAF A   G+ VSCSAGNSGPN  +  N+APWI+TVGA T+DR+F A    G+G
Sbjct: 304 TDSIAIGAFGATRHGIVVSCSAGNSGPNPETATNIAPWILTVGASTVDREFAANAITGDG 363

Query: 370 KRFTGVSLYSGQGMGSKPVELVYNKGSNTSSNMCLPGSLEPSVVRGKVVVCDRGINARVE 429
           K FTG SLY+G+ +    + LVY+   +  S +C PG L  S+V GK+V+CDRG NARVE
Sbjct: 364 KVFTGTSLYAGESLPDSQLSLVYS--GDCGSRLCYPGKLNSSLVEGKIVLCDRGGNARVE 423

Query: 430 KGGVVRDAGGIGMILANTAASGEELVADSHLLPAVAVGRKTGDLIRQYVRSISNPTAVLS 489
           KG  V+ AGG GMILANTA SGEEL ADSHL+PA  VG K GD IR Y+++  +PTA +S
Sbjct: 424 KGSAVKLAGGAGMILANTAESGEELTADSHLVPATMVGAKAGDQIRDYIKTSDSPTAKIS 483

Query: 490 FGGTILNVR-PSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLDNDKR 549
           F GT++    PSP VAAFSSRGPN +TP ILKPDVI PGVNILA W+  +GPT LD D R
Sbjct: 484 FLGTLIGPSPPSPRVAAFSSRGPNHLTPVILKPDVIAPGVNILAGWTGMVGPTDLDIDPR 543

Query: 550 KTQFNIMSGTSMSCPHISGLAALLKAAHPQWSPSAIKSALMTTAYTQDNTNSSLRDAAGG 609
           + QFNI+SGTSMSCPH+SGLAALL+ AHP WSP+AIKSAL+TTAY  +N+   + D A G
Sbjct: 544 RVQFNIISGTSMSCPHVSGLAALLRKAHPDWSPAAIKSALVTTAYDVENSGEPIEDLATG 603

Query: 610 GFSNPWAHGAGHVDPHKALSPGLLYDISTDDYIAFLCSLDYGIDHVQAIVKRSNI--TC- 669
             SN + HGAGHVDP+KAL+PGL+YDI   +Y+AFLC++ Y    +   ++   +   C 
Sbjct: 604 KSSNSFIHGAGHVDPNKALNPGLVYDIEVKEYVAFLCAVGYEFPGILVFLQDPTLYDACE 663

Query: 670 SRKFADPGQLNYPSFSVVFGSK-RVVRYTRIVTNVGA-AGSVYEVATTAPSVVNVTVKPS 729
           + K    G LNYPSFSVVF S   VV+Y R+V NVG+   +VYEV   +P+ V + V PS
Sbjct: 664 TSKLRTAGDLNYPSFSVVFASTGEVVKYKRVVKNVGSNVDAVYEVGVKSPANVEIDVSPS 723

Query: 730 KLVFTKVGERKRYTVTF---VASRDAAQTTRFGFGSIEWSNDQHQVRSPVAFAW 767
           KL F+K      Y VTF   V            FGSIEW++ +H V+SPVA  W
Sbjct: 724 KLAFSKEKSVLEYEVTFKSVVLGGGVGSVPGHEFGSIEWTDGEHVVKSPVAVQW 769

BLAST of Lsi06G003400 vs. TAIR 10
Match: AT5G51750.1 (subtilase 1.3 )

HSP 1 Score: 738.8 bits (1906), Expect = 4.4e-213
Identity = 391/775 (50.45%), Postives = 514/775 (66.32%), Query Frame = 0

Query: 8   FLLLLLLLLLLLLLLPCVF-VNAKKTYIVRMKHNALPSQYLTHHDWYSAHLQSLSSSSPS 67
           FL ++L + L+ L       ++ KKTY++ M  +A+P  Y  H  WYS+ + S++     
Sbjct: 11  FLFIILSINLIFLQAETTTQISTKKTYVIHMDKSAMPLPYTNHLQWYSSKINSVTQHKSQ 70

Query: 68  DS------LLYTYTSAYHGFAASLDSDEAELLRQSDSVLGVYEDTVYNLHTTRTPGFLGL 127
           +       +LYTY +A+HG AA L  +EAE L + D V+ V  +T Y LHTTR+P FLGL
Sbjct: 71  EEEGNNNRILYTYQTAFHGLAAQLTQEEAERLEEEDGVVAVIPETRYELHTTRSPTFLGL 130

Query: 128 DSDFGLWEGHNTQDLNQASHDVIIGVLDTGIWPESKSFDDAGMPEIPSRWRGECESGPDF 187
           +      E           HDV++GVLDTGIWPES+SF+D GM  +P+ WRG CE+G  F
Sbjct: 131 ERQ----ESERVWAERVTDHDVVVGVLDTGIWPESESFNDTGMSPVPATWRGACETGKRF 190

Query: 188 NPSLCNKKLIGARSFSKGYQMASGGGYFRKPRENESPRDQDGHGTHTASTAAGSHVANAS 247
               CN+K++GAR F +GY+ A+  G   +  E +SPRD+DGHGTHTA+T AGS V  A+
Sbjct: 191 LKRNCNRKIVGARVFYRGYEAAT--GKIDEELEYKSPRDRDGHGTHTAATVAGSPVKGAN 250

Query: 248 LLGYARGIARGMAPQARVAAYKTCWPTGCFGSDILAGMDRAISDGVDVLSLSLGGGSAPY 307
           L G+A G ARGMA +ARVAAYK CW  GCF SDIL+ +D+A++DGV VLS+SLGGG + Y
Sbjct: 251 LFGFAYGTARGMAQKARVAAYKVCWVGGCFSSDILSAVDQAVADGVQVLSISLGGGVSTY 310

Query: 308 YRDTIAIGAFAAMEKGVFVSCSAGNSGPNKASLANVAPWIMTVGAGTLDRDFPAYVQLGN 367
            RD+++I  F AME GVFVSCSAGN GP+  SL NV+PWI TVGA T+DRDFPA V++G 
Sbjct: 311 SRDSLSIATFGAMEMGVFVSCSAGNGGPDPISLTNVSPWITTVGASTMDRDFPATVKIGT 370

Query: 368 GKRFTGVSLYSGQGM--GSKPVELVY---NKGSNTSSNMCLPGSLEPSVVRGKVVVCDRG 427
            + F GVSLY G+ +   +K   LVY   N  S   ++ CL G+L+   V GK+V+CDRG
Sbjct: 371 MRTFKGVSLYKGRTVLPKNKQYPLVYLGRNASSPDPTSFCLDGALDRRHVAGKIVICDRG 430

Query: 428 INARVEKGGVVRDAGGIGMILANTAASGEELVADSHLLPAVAVGRKTGDLIRQYVRSISN 487
           +  RV+KG VV+ AGGIGM+L NTA +GEELVADSH+LPAVAVG K G LI+QY  +   
Sbjct: 431 VTPRVQKGQVVKRAGGIGMVLTNTATNGEELVADSHMLPAVAVGEKEGKLIKQYAMTSKK 490

Query: 488 PTAVLSFGGTILNVRPSPVVAAFSSRGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGL 547
            TA L   GT + ++PSPVVAAFSSRGPN ++ +ILKPD++ PGVNILAAW+  + P+ L
Sbjct: 491 ATASLEILGTRIGIKPSPVVAAFSSRGPNFLSLEILKPDLLAPGVNILAAWTGDMAPSSL 550

Query: 548 DNDKRKTQFNIMSGTSMSCPHISGLAALLKAAHPQWSPSAIKSALMTTAYTQDNTNSSLR 607
            +D R+ +FNI+SGTSMSCPH+SG+AAL+K+ HP WSP+AIKSALMTTAY  DN    L 
Sbjct: 551 SSDPRRVKFNILSGTSMSCPHVSGVAALIKSRHPDWSPAAIKSALMTTAYVHDNMFKPLT 610

Query: 608 DAAGGGFSNPWAHGAGHVDPHKALSPGLLYDISTDDYIAFLCSLDYGIDHVQAIVKRSNI 667
           DA+G   S+P+ HGAGH+DP +A  PGL+YDI   +Y  FLC+ D     ++   K SN 
Sbjct: 611 DASGAAPSSPYDHGAGHIDPLRATDPGLVYDIGPQEYFEFLCTQDLSPSQLKVFTKHSNR 670

Query: 668 TCSRKFA-DPGQLNYPSFSVVFGSK---RVVRYTRIVTNVGAAGSVYEVATTAPSVVNVT 727
           TC    A +PG LNYP+ S +F      + +   R VTNVG   S Y+V+ +     +VT
Sbjct: 671 TCKHTLAKNPGNLNYPAISALFPENTHVKAMTLRRTVTNVGPHISSYKVSVSPFKGASVT 730

Query: 728 VKPSKLVFTKVGERKRYTVTFVASRDAAQTTRFGFGSIEWSNDQHQVRSPVAFAW 767
           V+P  L FT   ++  YTVTF   R   +  R  FG + W +  H+VRSPV   W
Sbjct: 731 VQPKTLNFTSKHQKLSYTVTF---RTRFRMKRPEFGGLVWKSTTHKVRSPVIITW 776

BLAST of Lsi06G003400 vs. TAIR 10
Match: AT3G14240.1 (Subtilase family protein )

HSP 1 Score: 734.6 bits (1895), Expect = 8.4e-212
Identity = 384/749 (51.27%), Postives = 511/749 (68.22%), Query Frame = 0

Query: 32  TYIVRMKHNALPSQYLTHHDWYSAHLQSLSSSSPSDSLLYTYTSAYHGFAASLDSDEAEL 91
           TYIV + H A PS + TH  WY++ L SL+SS P  S+++TY + +HGF+A L S +A  
Sbjct: 27  TYIVHVDHEAKPSIFPTHFHWYTSSLASLTSSPP--SIIHTYDTVFHGFSARLTSQDASQ 86

Query: 92  LRQSDSVLGVYEDTVYNLHTTRTPGFLGLDSDFGLWEGHNTQDLNQASHDVIIGVLDTGI 151
           L     V+ V  + V +LHTTR+P FLGL S     +     + +    D++IGV+DTG+
Sbjct: 87  LLDHPHVISVIPEQVRHLHTTRSPEFLGLRST----DKAGLLEESDFGSDLVIGVIDTGV 146

Query: 152 WPESKSFDDAGMPEIPSRWRGECESGPDFNPSLCNKKLIGARSFSKGYQMASGGGYFRKP 211
           WPE  SFDD G+  +P +W+G+C +  DF  S CN+KL+GAR F  GY+  +  G   + 
Sbjct: 147 WPERPSFDDRGLGPVPIKWKGQCIASQDFPESACNRKLVGARFFCGGYEATN--GKMNET 206

Query: 212 RENESPRDQDGHGTHTASTAAGSHVANASLLGYARGIARGMAPQARVAAYKTCWPTGCFG 271
            E  SPRD DGHGTHTAS +AG +V  AS LGYA G+A GMAP+AR+AAYK CW +GC+ 
Sbjct: 207 TEFRSPRDSDGHGTHTASISAGRYVFPASTLGYAHGVAAGMAPKARLAAYKVCWNSGCYD 266

Query: 272 SDILAGMDRAISDGVDVLSLSLGGGSAPYYRDTIAIGAFAAMEKGVFVSCSAGNSGPNKA 331
           SDILA  D A++DGVDV+SLS+GG   PYY D IAIGAF A+++G+FVS SAGN GP   
Sbjct: 267 SDILAAFDTAVADGVDVISLSVGGVVVPYYLDAIAIGAFGAIDRGIFVSASAGNGGPGAL 326

Query: 332 SLANVAPWIMTVGAGTLDRDFPAYVQLGNGKRFTGVSLYSGQGMG-SKPVELVYN----K 391
           ++ NVAPW+ TVGAGT+DRDFPA V+LGNGK  +GVS+Y G G+   +   LVY      
Sbjct: 327 TVTNVAPWMTTVGAGTIDRDFPANVKLGNGKMISGVSVYGGPGLDPGRMYPLVYGGSLLG 386

Query: 392 GSNTSSNMCLPGSLEPSVVRGKVVVCDRGINARVEKGGVVRDAGGIGMILANTAASGEEL 451
           G   SS++CL GSL+P++V+GK+V+CDRGIN+R  KG +VR  GG+GMI+AN    GE L
Sbjct: 387 GDGYSSSLCLEGSLDPNLVKGKIVLCDRGINSRATKGEIVRKNGGLGMIIANGVFDGEGL 446

Query: 452 VADSHLLPAVAVGRKTGDLIRQYV------RSISNPTAVLSFGGTILNVRPSPVVAAFSS 511
           VAD H+LPA +VG   GD IR+Y+      RS  +PTA + F GT L +RP+PVVA+FS+
Sbjct: 447 VADCHVLPATSVGASGGDEIRRYISESSKSRSSKHPTATIVFKGTRLGIRPAPVVASFSA 506

Query: 512 RGPNLVTPQILKPDVIGPGVNILAAWSESIGPTGLDNDKRKTQFNIMSGTSMSCPHISGL 571
           RGPN  TP+ILKPDVI PG+NILAAW + IGP+G+ +D R+T+FNI+SGTSM+CPH+SGL
Sbjct: 507 RGPNPETPEILKPDVIAPGLNILAAWPDRIGPSGVTSDNRRTEFNILSGTSMACPHVSGL 566

Query: 572 AALLKAAHPQWSPSAIKSALMTTAYTQDNTNSSLRDAAGGGFSNPWAHGAGHVDPHKALS 631
           AALLKAAHP WSP+AI+SAL+TTAYT DN+   + D + G  S+   +G+GHV P KA+ 
Sbjct: 567 AALLKAAHPDWSPAAIRSALITTAYTVDNSGEPMMDESTGNTSSVMDYGSGHVHPTKAMD 626

Query: 632 PGLLYDISTDDYIAFLCSLDYGIDHVQAIVKR-SNITCSRKFADPGQLNYPSFSVVF--- 691
           PGL+YDI++ DYI FLC+ +Y   ++  I +R ++   +R+    G LNYPSFSVVF   
Sbjct: 627 PGLVYDITSYDYINFLCNSNYTRTNIVTITRRQADCDGARRAGHVGNLNYPSFSVVFQQY 686

Query: 692 -GSKRVVRYTRIVTNVGAAGSVYEVATTAPSVVNVTVKPSKLVFTKVGERKRYTVTFVAS 751
             SK    + R VTNVG + SVYE+    P    VTV+P KL F +VG++  + V    +
Sbjct: 687 GESKMSTHFIRTVTNVGDSDSVYEIKIRPPRGTTVTVEPEKLSFRRVGQKLSFVVRVKTT 746

Query: 752 --RDAAQTTRFGFGSIEWSNDQHQVRSPV 763
             + +   T    G I WS+ +  V SP+
Sbjct: 747 EVKLSPGATNVETGHIVWSDGKRNVTSPL 767

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9ZUF60.0e+0071.91Subtilisin-like protease SBT1.8 OS=Arabidopsis thaliana OX=3702 GN=SBT1.8 PE=1 S... [more]
O653512.1e-23155.57Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana OX=3702 GN=SBT1.7 PE=1 S... [more]
Q9LVJ15.8e-21853.36Subtilisin-like protease SBT1.4 OS=Arabidopsis thaliana OX=3702 GN=SBT1.4 PE=2 S... [more]
Q9FLI46.2e-21250.45Subtilisin-like protease SBT1.3 OS=Arabidopsis thaliana OX=3702 GN=SBT1.3 PE=2 S... [more]
Q9LUM31.2e-21051.27Subtilisin-like protease SBT1.5 OS=Arabidopsis thaliana OX=3702 GN=SBT1.5 PE=2 S... [more]
Match NameE-valueIdentityDescription
A0A6J1JIF30.0e+0094.67subtilisin-like protease SBT1.8 OS=Cucurbita maxima OX=3661 GN=LOC111484737 PE=3... [more]
A0A6J1H8F10.0e+0094.41subtilisin-like protease SBT1.8 OS=Cucurbita moschata OX=3662 GN=LOC111461488 PE... [more]
A0A5A7UKV30.0e+0095.38Subtilisin-like protease SBT1.8 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_... [more]
A0A1S3BQE80.0e+0095.38subtilisin-like protease SBT1.8 OS=Cucumis melo OX=3656 GN=LOC103492374 PE=3 SV=... [more]
A0A5D3CDV90.0e+0095.50Subtilisin-like protease SBT1.8 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_... [more]
Match NameE-valueIdentityDescription
XP_038879224.10.0e+0096.36subtilisin-like protease SBT1.8 [Benincasa hispida][more]
XP_023516159.10.0e+0094.54subtilisin-like protease SBT1.8 [Cucurbita pepo subsp. pepo][more]
XP_022987083.10.0e+0094.67subtilisin-like protease SBT1.8 [Cucurbita maxima][more]
XP_022960792.10.0e+0094.41subtilisin-like protease SBT1.8 [Cucurbita moschata] >KAG7023704.1 Subtilisin-li... [more]
KAG6590040.10.0e+0094.28Subtilisin-like protease 1.8, partial [Cucurbita argyrosperma subsp. sororia][more]
Match NameE-valueIdentityDescription
AT2G05920.10.0e+0071.91Subtilase family protein [more]
AT5G67360.11.5e-23255.57Subtilase family protein [more]
AT3G14067.14.1e-21953.36Subtilase family protein [more]
AT5G51750.14.4e-21350.45subtilase 1.3 [more]
AT3G14240.18.4e-21251.27Subtilase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR015500Peptidase S8, subtilisin-relatedPRINTSPR00723SUBTILISINcoord: 219..232
score: 49.97
coord: 548..564
score: 56.45
coord: 139..158
score: 29.87
IPR041469Subtilisin-like protease, fibronectin type-III domainPFAMPF17766fn3_6coord: 666..763
e-value: 1.5E-27
score: 95.7
IPR000209Peptidase S8/S53 domainPFAMPF00082Peptidase_S8coord: 140..590
e-value: 3.1E-49
score: 168.2
NoneNo IPR availableGENE3D2.60.40.2310coord: 636..766
e-value: 5.2E-37
score: 128.5
NoneNo IPR availableGENE3D3.50.30.30coord: 349..487
e-value: 5.5E-187
score: 624.2
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 203..230
NoneNo IPR availablePANTHERPTHR10795:SF653CUCUMISIN-RELATEDcoord: 22..766
NoneNo IPR availablePROSITEPS51892SUBTILASEcoord: 123..619
score: 28.259632
NoneNo IPR availableCDDcd02120PA_subtilisin_likecoord: 355..478
e-value: 9.4063E-40
score: 140.627
NoneNo IPR availableSUPERFAMILY54897Protease propeptides/inhibitorscoord: 31..108
IPR003137PA domainPFAMPF02225PAcoord: 381..465
e-value: 4.5E-10
score: 39.5
IPR010259Peptidase S8 propeptide/proteinase inhibitor I9PFAMPF05922Inhibitor_I9coord: 32..110
e-value: 2.0E-14
score: 54.0
IPR036852Peptidase S8/S53 domain superfamilyGENE3D3.40.50.200Peptidase S8/S53 domaincoord: 141..632
e-value: 5.5E-187
score: 624.2
IPR036852Peptidase S8/S53 domain superfamilySUPERFAMILY52743Subtilisin-likecoord: 109..623
IPR037045Peptidase S8 propeptide/proteinase inhibitor I9 superfamilyGENE3D3.30.70.80Peptidase S8 propeptide/proteinase inhibitor I9coord: 27..110
e-value: 2.8E-18
score: 68.1
IPR045051Subtilisin-like proteasePANTHERPTHR10795PROPROTEIN CONVERTASE SUBTILISIN/KEXINcoord: 22..766
IPR023828Peptidase S8, subtilisin, Ser-active sitePROSITEPS00138SUBTILASE_SERcoord: 549..559
IPR034197Cucumisin-like catalytic domainCDDcd04852Peptidases_S8_3coord: 107..585
e-value: 6.82853E-147
score: 430.481

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi06G003400.1Lsi06G003400.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
biological_process GO:0009610 response to symbiotic fungus
molecular_function GO:0004252 serine-type endopeptidase activity
molecular_function GO:0008236 serine-type peptidase activity