Lsi06G003350 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi06G003350
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionTubulin--tyrosine ligase-like protein 12
Locationchr06: 3706863 .. 3712810 (+)
RNA-Seq ExpressionLsi06G003350
SyntenyLsi06G003350
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TCCAAGCCCACAGCGATTGAGCATCTTCCTCAGTGCGGTGGCCGAGCTCAGTTCAAAGCCGCCATTGAACGGCAAAGGACAGCGAAAATTCACGTCGAGAAATGGCCGACGGAAAAAGAATCCAAACCTTTGAGGATTTCGTCGAAGTTCATGGTTTGCTCCTCACTGCCTCTGGACTTCCCCAGTCTCTGCACCGTCAACTGTTTCAGAAACTGACCTCAGAGACCTTCGACGGCGGCGCTCACTTCCAAGTCGAACCGTTTGAGGATGGACGCTGTAGACGCCTCGTCCTCTCCTCAGACTTCATGGCCAAGGAGTCGCACGTCTTCCTTGTCGACCATGCCTGGACTTTCCGTCTCTCCGACGCTTACACGCAGGTTAGGTTCTTATACTGAATTTGTTACTTCAATTCGAAGGCTTCTGAAGTGAGCTACTCTTATTTAGTTGTTAACTTGGAAATGCTTGCGTGCGGACAGTTGTTGGAAGTGCCTGGATTGGCCGAGAGAATGGCGTCCTTGATGTGTGTGGATGTTGATATGAATTTGGATGAGGAAGAGGAAGATCAATCAAAATCAAATGATGATGAAGCTAAACAGAGTGTTTGGGAACTATTGGAGAGTGAAATACGCGGTGCGAAAGAAAAAGGAAATGACTCTGTTAGATGGTTGGAGCTTGAGGATCTTCAAATTGAAGATGATACACTTTTGTCCCTCGATTTGCCAACTAAGTTTCCGGTAACTTTATCATTTGAGCAAACAATTTGACATAAAATGCTATCATCGGAAGCTAATGTGAACTCAATGTTGAAATGGTTGTATTGCGTCGATTATAATTGCAATGTTAATGATTTCCTTTGAATATTGTAGGAAAGTAGAACATGAAATTCAAATTATGATGTTAGCATTTTTCTGGAGAATGTGAAGCATTTTAACTATATTAAAATACTATCATATAGGAGTTGTATTGAAATTGTTAGATATATGATATTGCATTCTGAATATTTTCAACATTTTTATTTAAACTAATGTTGGAAAGAGATAAAACTTTCTCCTAATATCACTTTCTAGTATCGAAGCTATATGGATATCTTCATATAAATATGTAGAAGATGTCAGTATTTCATTTCTCAATCAGTTCTGTTCGCTCACTAGTTTTAAATGTGTGCTATATTTCTTATGATTTAGGATTTACTTGCGCTTAGCTTGACTGGAAACAAGCTTAGAAATGTAGACGTGATCGCCCAGGAAGTTGCTAAATTTAAACATCTTAGAGCACTTTGGTTGAACGATAATCCAGTTGCAGAAAACTGGTACGTTTGATGGTCTAGTGATGCATAATACTTTTGGGTCTTCATGCTTAAATTAATGAATGTCCTGCTGAAGTTTTCTGAACTCTTCCTTTTCAGTGATGAGAACCTGCAGCAGAAAGTTCTTGAAGGATCTCCAAATTTAGAAATCTACAACTCACGATTTACTCTGAACTTTTCCAAGTGGGCATTGGGATTCTGTGGAGATATGTATGGGAAGGATAATCCTGGAAGCATCTACCCAGCTGATCACACATTGCAATGCTTGACAAGTCTAGACCTTTCAAGTAGATATATTCACAACTTAATCAACAAGGTAAGAATTTCACAACTGTTTTGGTTAGCATGTTATAATCTTGAAGGAAATAGGAACTCTTTGTTTTGGCTAGCACTGACTTTGTTTTCATCAAAGGGATGACAGAAAGTTCGACCAATGTTTAAGGCATTCAAATGTATCAGCTATGACCATTAGTTAGTTTCACATAGTTTGATAATCCTCTTAGTCATCCTTCTCAGGCCTTTTCACCCGTTGAGCTGCCATCTCTCTTGTACTTGAATCTTAAGGGAAACCCCTTGGAACAGAATTCTGTTGGTGACTTGTTGAAAGTCCTGAAGGAATTTCCTTGCTTAAGTTCTTTGGAGGTATTTTGTCAAAATTGGTCTATCTGCTATGGAATCCTGCTCTAAATGTCTGTTTGGAAAGATCTATTAATCTTGCACTGTTCTTATTTGGTTATGCAGGTGGATATTCCTGGTCCTCTTGGAGAGAAGGCTTCAGACATAATTGAATCTCTCCCTAATCTGAATGCTCTGAATGGCATTGACGTGGCAAAAATATTGAATTCTGGAAAGCATGTGATTGATTCGATGCTTCTGCCACGTCTCCCTGAGTGGGCTCCTGAGGAGCCTCTCCCTGATCGTGTTATAAATGCGATGTGGCAATATTTGATGACCTACAGACTAGCAGATGAGGAAAAGATTGATGAAACCTCTGTTTGGTATGCTGTTTATCGCCTAGGTTTGAGTTACTTTTCCATCTTTTCCAGAATGTACATGTTTTAATCTTTTATCTGGAGCTCTTGGCAGGTACGTGATGGATGAGCTAGGTTCAGCTTTGCGGCATAGTGATGAGCCAAATTTCAGAGTGGCTCCTTTCCTCTTCATGCCAGAGGGAGAGCTGACGTCCGCCATAAGGTTTCTTTATTATCTATTTCTTCCCCCTTCATTGTATAAGAACATGCTCTTTTTATGTATTATTGTGCCTTTGTGAAAATAACCCTTTAATTCTGTTTTCCAGCTTTACCATATTATGGCCAATCCACAATGTTCAGAAAGGTGATGAATGCACTCGTGATTATCTGTTTGGCTTTGGGGAGGACAAACAACGGTCCGCTAGACTTACAGCTTGGTTTCACACCCCGCAAAACTATTTTGTGCATGTACTTTCCTACTTATGAATCTCTTATTCATTTAACTGTTAAAGCTGTCACGGGGCATTGCTAAGTTTGAATCTAATCTAGAGAAACTATATCAGGAGTATGAGAAACACATCAGGAATCTGCAGTCGAAAGCGTTGGCTTCATCTATTTCTCAAACCACGTCAAACCCCGAAGGACTACATCAGAGCAAAGGAGGCACTTTTCATGTTTATACTGACAATCCTCAAGTTGAAGAATTTTTAAACCGCCCTGAATTTATAATGAGTATGTACTGCAACCGTTTTCAATTATGTCAGAATACTTTAGTATGTAGCTCTACTAATTTAGATTGAGCTAAAGTAACTTCCAAAAAATCAATCGCATCAGGAGTATGGTTATTTAGGTTTTTTTTTTAGCTCCAAGAATTGTACTTCACTCAGTATGTTGATCATTTTCTTAGATGGTCATAAATTTCTTTGTTCAATTTATTTGCTTCCAGCTAGTGATCCAAAAGAGGCAGATATCATATGGACAAGTATGCAGATTGATGAGGATACAAGAAAGGCTACAGGGATAACAGATCAGCAATATGTAAACCAATTTCCCTTCGAAGCTTGTCTAGTCATGAAACATCATTTGGCAGAGACTGTTGAGAAGGTACAGAATGGGAACTAGCTCATATATATATATATATATATATATATATATTTATATATATTGGAATTAATTTAATTCTTATTTCTTTCTGCCAAATGCTAAAACATAGAAATTTCACTTTTCCAACAGGCACATGGATGTCCTGAGTGGTTGCAGCCTACATACAATCTCGAGACACATTTATCTCAACTTATTGGTGATTATTTGGTGCGTAAAAGAGATGGATTAAATAATTTATGGATACTGAAACCATGGAATATGGCACGGACTATCGATACTACAGTCACCGATAACCTATCTGCTATTATCCGTCTCATGGAAACTGGTCCTAAGATATGTCAAAAGTATATTGAGCATCCTGCTCTGTTTAACGGGAAGAAGTTTGATCTGCGTTATATTGTACTTGTACGCAGTATGAAGCCTTTGGAAATTTTCCTCGCAGATTCTTTTTGGGTATGTGAATTCTTCTTTCATCTTTCTTGAACAAACTATGTCAAAGTCCATACTCAATCCCTTCCCTCTCTTCAGGTTCGATTGGCGAATAACCCATACTCTTTAGAGACACAGAGTCTGTTTGAGTACGAGACTCACTTTACGGTTATGGTATGCTCTAATTACCATCATTTGTGACAAAATTTATGTAGTTTTATCTTGCAGTCAGTGTAGCTTGCAATAGTTCTTGGTGCGCCAGAGAGATCGATTACAATGCTGTTTCATTTTAGACCACTTATTTACTCATATGCTTCACTTGCAGAACTACCGTGGAAAATTAAATCATAAAAATATATCCGATTTTGTCAAGGAATTTGAGCAAGAGCACAATGGTATGCAAGCTTTTAATTTTGCTTTCCTTGCCCTTTAGGGTGTTCCTTTTGATTCTTTTGTTCAATATATATACCTGTTTCTTATTTAAAAAAATGCTTTTAATTTATCAACGAGCTTTCCTTTCATGTTATATTAATACTCTAAATTAGCTAAAGCTTTATACCGTGATGAGAATATTGCAGTTAAGTGGTTGGATATCCATTCGAGAGTGAGAAGCATGATCCGATCAGTTTTCGAGTCAGCTATGGTGGTTCACCCTGAGATGCATAGTCCATTCTCTAGGGCCATATATGGTTTGGATGTGATGCTTGATTCTTCTTTTCAACCAAAGTTATTGGAGGTTTGTACTAATACTATCTGTTTTCATTATTTGGGTTGCGTTTTCATCATCATATCAAATGATCATATTTATAGTAGAAGTGCCAAAACAAAAGAAAAAAGCTTAAAATGACTGAAACCAAAATCTGTCTCTGTTGGAACTGAGTCAGTTTGGCAGTTTTGGAAAACCTAATATTTTCAGTTAGTTTTTGTTTAAAAAGTGGCTAAACCGAATATACCTAATCGGACTGAACAATGTGTATTAAAAAAATGAAATATGTTATCAATGGTGGTGGTCAAGGTGACCAATAAAACCCTACCTAATAGCGATATAGTTGGAGAGGTAACAAAACCCTATATCATTTCAAAACCGATAAACCTAAAAAAATGGATTATTTTGTGAAAGAACTTTTAGGCCTATAAAAAGTGGGATTTGTGCTTGTGGAAATTATCGAGTAATCGGAGATAAAAAAGAAGATTTGAAATTTTGTGAACAATGTGGATGTAATGTGGATGTAAAATGCAATATGATATTCTTGAGGTGATGAGTTTGACCGGGAATGCATCATTTTTATCTATATATCTTGATACTTTTCATCCAGCTCATTGGCCCAATTGATTGCGGGTTCAGGACCCATTCCCTAGGCCCAACTCTTTGTAAAGTGCATGGTTTAACCAGACAACAAACTGCTAGACCGAATCGACTTACCTTTTGGTTAATCGATTTGAGGGTTTGGTTATGTATCTTTAGCAAATCGGTTCAATTTGCTATAATTCTTTATATATTTGGCGATTCAGCTTCCATTCGACTCTCAAATTGAACTGAATCAAAACAAACCAAACCGAAGTGATTTCACCCCTAATTTATGAATACTGCCTTCATGCTTTCTTTTTTGTTACCAGTCCATTTTGCTATCAATTGTCTATGCTGTTTTACCAATGTTGCAATGTTCATCTCACTTGTTTAGGTGACTTACTGCCCTGATTGCACCCGAGCTTGCAAGTATGACGTGGAAAATGTTTATGGAGGAGTTATAAAGGGCAAAGAATTTTACAACGATGTTTTTGGCTGTCTCTTCTTAAATGAAACCACTCATGTCACTCCATTATAATCAAACCACCACATTTCAATTTTGTTACGGTAGGTACTGGTAGCTTTTATTTATTATTTTTCTCCCCCTTGAGGCTTTGTATCATTATTTTTCATTTCATGTTTTGAGTTATAACTTATAATGTAATCAAATTGAGGCTTTGTATCATTATTTTTCATTTCATTATTTTTTACCCTTTCTTGCACCATCAAATTGAAATTACCGAAACGACCTTATTGTGCACGCATGTTGGGATCTCGACTTTTGTTACATTGGGTATTTGACAGCAAGAAAGTGAGAGAAAATAGGGTTTGAATAAAGCTTAAAACAAAA

mRNA sequence

TCCAAGCCCACAGCGATTGAGCATCTTCCTCAGTGCGGTGGCCGAGCTCAGTTCAAAGCCGCCATTGAACGGCAAAGGACAGCGAAAATTCACGTCGAGAAATGGCCGACGGAAAAAGAATCCAAACCTTTGAGGATTTCGTCGAAGTTCATGGTTTGCTCCTCACTGCCTCTGGACTTCCCCAGTCTCTGCACCAAACTGACCTCAGAGACCTTCGACGGCGGCGCTCACTTCCAAGTCGAACCGTTTGAGGATGGACGCTGTAGACGCCTCGTCCTCTCCTCAGACTTCATGGCCAAGGAGTCGCACGTCTTCCTTGTCGACCATGCCTGGACTTTCCGTCTCTCCGACGCTTACACGCAGTTGTTGGAAGTGCCTGGATTGGCCGAGAGAATGGCGTCCTTGATGTGTGTGGATGTTGATATGAATTTGGATGAGGAAGAGGAAGATCAATCAAAATCAAATGATGATGAAGCTAAACAGAGTGTTTGGGAACTATTGGAGAGTGAAATACGCGGTGCGAAAGAAAAAGGAAATGACTCTGTTAGATGGTTGGAGCTTGAGGATCTTCAAATTGAAGATGATACACTTTTGTCCCTCGATTTGCCAACTAAGTTTCCGGATTTACTTGCGCTTAGCTTGACTGGAAACAAGCTTAGAAATGTAGACGTGATCGCCCAGGAAGTTGCTAAATTTAAACATCTTAGAGCACTTTGGTTGAACGATAATCCAGTTGCAGAAAACTGGTACTTTTCTGAACTCTTCCTTTTCAGTGATGAGAACCTGCAGCAGAAAGTTCTTGAAGGATCTCCAAATTTAGAAATCTACAACTCACGATTTACTCTGAACTTTTCCAAGTGGGCATTGGGATTCTGTGGAGATATGTATGGGAAGGATAATCCTGGAAGCATCTACCCAGCTGATCACACATTGCAATGCTTGACAAGTCTAGACCTTTCAAGTAGATATATTCACAACTTAATCAACAAGGCCTTTTCACCCGTTGAGCTGCCATCTCTCTTGTACTTGAATCTTAAGGGAAACCCCTTGGAACAGAATTCTGTTGGTGACTTGTTGAAAGTCCTGAAGGAATTTCCTTGCTTAAGTTCTTTGGAGGTGGATATTCCTGGTCCTCTTGGAGAGAAGGCTTCAGACATAATTGAATCTCTCCCTAATCTGAATGCTCTGAATGGCATTGACGTGGCAAAAATATTGAATTCTGGAAAGCATGTGATTGATTCGATGCTTCTGCCACGTCTCCCTGAGTGGGCTCCTGAGGAGCCTCTCCCTGATCGTGTTATAAATGCGATGTGGCAATATTTGATGACCTACAGACTAGCAGATGAGGAAAAGATTGATGAAACCTCTGTTTGGTATGCTGTTTATCGCCTAGGTTTGAGTTACTTTTCCATCTTTTCCAGAATGTACGTGATGGATGAGCTAGGTTCAGCTTTGCGGCATAGTGATGAGCCAAATTTCAGAGTGGCTCCTTTCCTCTTCATGCCAGAGGGAGAGCTGACGTCCGCCATAAGCTTTACCATATTATGGCCAATCCACAATGTTCAGAAAGGTGATGAATGCACTCGTGATTATCTGTTTGGCTTTGGGGAGGACAAACAACGGTCCGCTAGACTTACAGCTTGGTTTCACACCCCGCAAAACTATTTTGTGCATGAGTATGAGAAACACATCAGGAATCTGCAGTCGAAAGCGTTGGCTTCATCTATTTCTCAAACCACGTCAAACCCCGAAGGACTACATCAGAGCAAAGGAGGCACTTTTCATGTTTATACTGACAATCCTCAAGTTGAAGAATTTTTAAACCGCCCTGAATTTATAATGACTAGTGATCCAAAAGAGGCAGATATCATATGGACAAGTATGCAGATTGATGAGGATACAAGAAAGGCTACAGGGATAACAGATCAGCAATATGTAAACCAATTTCCCTTCGAAGCTTGTCTAGTCATGAAACATCATTTGGCAGAGACTGTTGAGAAGGCACATGGATGTCCTGAGTGGTTGCAGCCTACATACAATCTCGAGACACATTTATCTCAACTTATTGGTGATTATTTGGTGCGTAAAAGAGATGGATTAAATAATTTATGGATACTGAAACCATGGAATATGGCACGGACTATCGATACTACAGTCACCGATAACCTATCTGCTATTATCCGTCTCATGGAAACTGGTCCTAAGATATGTCAAAAGTATATTGAGCATCCTGCTCTGTTTAACGGGAAGAAGTTTGATCTGCGTTATATTGTACTTGTACGCAGTATGAAGCCTTTGGAAATTTTCCTCGCAGATTCTTTTTGGGTTCGATTGGCGAATAACCCATACTCTTTAGAGACACAGAGTCTGTTTGAGTACGAGACTCACTTTACGGTTATGTTTTATCTTGCAGTCAGTGTAGCTTGCAATAGTTCTTGGTGCGCCAGAGAGATCGATTACAATGCTTGGTTGGATATCCATTCGAGAGTGAGAAGCATGATCCGATCAGTTTTCGAGTCAGCTATGGTGGTTCACCCTGAGATGCATAGTCCATTCTCTAGGGCCATATATGGTTTGGATGTGATGCTTGATTCTTCTTTTCAACCAAAGTTATTGGAGGTGACTTACTGCCCTGATTGCACCCGAGCTTGCAAGTATGACGTGGAAAATGTTTATGGAGGAGTTATAAAGGGCAAAGAATTTTACAACGATGTTTTTGGCTGTCTCTTCTTAAATGAAACCACTCATGTCACTCCATTATAATCAAACCACCACATTTCAATTTTGTTACGGTAGGTACTGGTAGCTTTTATTTATTATTTTTCTCCCCCTTGAGGCTTTGTATCATTATTTTTCATTTCATGTTTTGAGTTATAACTTATAATGTAATCAAATTGAGGCTTTGTATCATTATTTTTCATTTCATTATTTTTTACCCTTTCTTGCACCATCAAATTGAAATTACCGAAACGACCTTATTGTGCACGCATGTTGGGATCTCGACTTTTGTTACATTGGGTATTTGACAGCAAGAAAGTGAGAGAAAATAGGGTTTGAATAAAGCTTAAAACAAAA

Coding sequence (CDS)

TCCAAGCCCACAGCGATTGAGCATCTTCCTCAGTGCGGTGGCCGAGCTCAGTTCAAAGCCGCCATTGAACGGCAAAGGACAGCGAAAATTCACGTCGAGAAATGGCCGACGGAAAAAGAATCCAAACCTTTGAGGATTTCGTCGAAGTTCATGGTTTGCTCCTCACTGCCTCTGGACTTCCCCAGTCTCTGCACCAAACTGACCTCAGAGACCTTCGACGGCGGCGCTCACTTCCAAGTCGAACCGTTTGAGGATGGACGCTGTAGACGCCTCGTCCTCTCCTCAGACTTCATGGCCAAGGAGTCGCACGTCTTCCTTGTCGACCATGCCTGGACTTTCCGTCTCTCCGACGCTTACACGCAGTTGTTGGAAGTGCCTGGATTGGCCGAGAGAATGGCGTCCTTGATGTGTGTGGATGTTGATATGAATTTGGATGAGGAAGAGGAAGATCAATCAAAATCAAATGATGATGAAGCTAAACAGAGTGTTTGGGAACTATTGGAGAGTGAAATACGCGGTGCGAAAGAAAAAGGAAATGACTCTGTTAGATGGTTGGAGCTTGAGGATCTTCAAATTGAAGATGATACACTTTTGTCCCTCGATTTGCCAACTAAGTTTCCGGATTTACTTGCGCTTAGCTTGACTGGAAACAAGCTTAGAAATGTAGACGTGATCGCCCAGGAAGTTGCTAAATTTAAACATCTTAGAGCACTTTGGTTGAACGATAATCCAGTTGCAGAAAACTGGTACTTTTCTGAACTCTTCCTTTTCAGTGATGAGAACCTGCAGCAGAAAGTTCTTGAAGGATCTCCAAATTTAGAAATCTACAACTCACGATTTACTCTGAACTTTTCCAAGTGGGCATTGGGATTCTGTGGAGATATGTATGGGAAGGATAATCCTGGAAGCATCTACCCAGCTGATCACACATTGCAATGCTTGACAAGTCTAGACCTTTCAAGTAGATATATTCACAACTTAATCAACAAGGCCTTTTCACCCGTTGAGCTGCCATCTCTCTTGTACTTGAATCTTAAGGGAAACCCCTTGGAACAGAATTCTGTTGGTGACTTGTTGAAAGTCCTGAAGGAATTTCCTTGCTTAAGTTCTTTGGAGGTGGATATTCCTGGTCCTCTTGGAGAGAAGGCTTCAGACATAATTGAATCTCTCCCTAATCTGAATGCTCTGAATGGCATTGACGTGGCAAAAATATTGAATTCTGGAAAGCATGTGATTGATTCGATGCTTCTGCCACGTCTCCCTGAGTGGGCTCCTGAGGAGCCTCTCCCTGATCGTGTTATAAATGCGATGTGGCAATATTTGATGACCTACAGACTAGCAGATGAGGAAAAGATTGATGAAACCTCTGTTTGGTATGCTGTTTATCGCCTAGGTTTGAGTTACTTTTCCATCTTTTCCAGAATGTACGTGATGGATGAGCTAGGTTCAGCTTTGCGGCATAGTGATGAGCCAAATTTCAGAGTGGCTCCTTTCCTCTTCATGCCAGAGGGAGAGCTGACGTCCGCCATAAGCTTTACCATATTATGGCCAATCCACAATGTTCAGAAAGGTGATGAATGCACTCGTGATTATCTGTTTGGCTTTGGGGAGGACAAACAACGGTCCGCTAGACTTACAGCTTGGTTTCACACCCCGCAAAACTATTTTGTGCATGAGTATGAGAAACACATCAGGAATCTGCAGTCGAAAGCGTTGGCTTCATCTATTTCTCAAACCACGTCAAACCCCGAAGGACTACATCAGAGCAAAGGAGGCACTTTTCATGTTTATACTGACAATCCTCAAGTTGAAGAATTTTTAAACCGCCCTGAATTTATAATGACTAGTGATCCAAAAGAGGCAGATATCATATGGACAAGTATGCAGATTGATGAGGATACAAGAAAGGCTACAGGGATAACAGATCAGCAATATGTAAACCAATTTCCCTTCGAAGCTTGTCTAGTCATGAAACATCATTTGGCAGAGACTGTTGAGAAGGCACATGGATGTCCTGAGTGGTTGCAGCCTACATACAATCTCGAGACACATTTATCTCAACTTATTGGTGATTATTTGGTGCGTAAAAGAGATGGATTAAATAATTTATGGATACTGAAACCATGGAATATGGCACGGACTATCGATACTACAGTCACCGATAACCTATCTGCTATTATCCGTCTCATGGAAACTGGTCCTAAGATATGTCAAAAGTATATTGAGCATCCTGCTCTGTTTAACGGGAAGAAGTTTGATCTGCGTTATATTGTACTTGTACGCAGTATGAAGCCTTTGGAAATTTTCCTCGCAGATTCTTTTTGGGTTCGATTGGCGAATAACCCATACTCTTTAGAGACACAGAGTCTGTTTGAGTACGAGACTCACTTTACGGTTATGTTTTATCTTGCAGTCAGTGTAGCTTGCAATAGTTCTTGGTGCGCCAGAGAGATCGATTACAATGCTTGGTTGGATATCCATTCGAGAGTGAGAAGCATGATCCGATCAGTTTTCGAGTCAGCTATGGTGGTTCACCCTGAGATGCATAGTCCATTCTCTAGGGCCATATATGGTTTGGATGTGATGCTTGATTCTTCTTTTCAACCAAAGTTATTGGAGGTGACTTACTGCCCTGATTGCACCCGAGCTTGCAAGTATGACGTGGAAAATGTTTATGGAGGAGTTATAAAGGGCAAAGAATTTTACAACGATGTTTTTGGCTGTCTCTTCTTAAATGAAACCACTCATGTCACTCCATTATAA

Protein sequence

SKPTAIEHLPQCGGRAQFKAAIERQRTAKIHVEKWPTEKESKPLRISSKFMVCSSLPLDFPSLCTKLTSETFDGGAHFQVEPFEDGRCRRLVLSSDFMAKESHVFLVDHAWTFRLSDAYTQLLEVPGLAERMASLMCVDVDMNLDEEEEDQSKSNDDEAKQSVWELLESEIRGAKEKGNDSVRWLELEDLQIEDDTLLSLDLPTKFPDLLALSLTGNKLRNVDVIAQEVAKFKHLRALWLNDNPVAENWYFSELFLFSDENLQQKVLEGSPNLEIYNSRFTLNFSKWALGFCGDMYGKDNPGSIYPADHTLQCLTSLDLSSRYIHNLINKAFSPVELPSLLYLNLKGNPLEQNSVGDLLKVLKEFPCLSSLEVDIPGPLGEKASDIIESLPNLNALNGIDVAKILNSGKHVIDSMLLPRLPEWAPEEPLPDRVINAMWQYLMTYRLADEEKIDETSVWYAVYRLGLSYFSIFSRMYVMDELGSALRHSDEPNFRVAPFLFMPEGELTSAISFTILWPIHNVQKGDECTRDYLFGFGEDKQRSARLTAWFHTPQNYFVHEYEKHIRNLQSKALASSISQTTSNPEGLHQSKGGTFHVYTDNPQVEEFLNRPEFIMTSDPKEADIIWTSMQIDEDTRKATGITDQQYVNQFPFEACLVMKHHLAETVEKAHGCPEWLQPTYNLETHLSQLIGDYLVRKRDGLNNLWILKPWNMARTIDTTVTDNLSAIIRLMETGPKICQKYIEHPALFNGKKFDLRYIVLVRSMKPLEIFLADSFWVRLANNPYSLETQSLFEYETHFTVMFYLAVSVACNSSWCAREIDYNAWLDIHSRVRSMIRSVFESAMVVHPEMHSPFSRAIYGLDVMLDSSFQPKLLEVTYCPDCTRACKYDVENVYGGVIKGKEFYNDVFGCLFLNETTHVTPL
Homology
BLAST of Lsi06G003350 vs. ExPASy Swiss-Prot
Match: Q3UDE2 (Tubulin--tyrosine ligase-like protein 12 OS=Mus musculus OX=10090 GN=Ttll12 PE=1 SV=1)

HSP 1 Score: 292.7 bits (748), Expect = 1.4e-77
Identity = 187/542 (34.50%), Postives = 270/542 (49.82%), Query Frame = 0

Query: 422 EWAPEEPLPD---RVINAMWQYLMTYRLADEEKIDETSVWYAVYRLGLSYFSIFSRMYVM 481
           E+  E P P+    V+  MW++  TY+LA     ++  VW                 Y+M
Sbjct: 147 EFHGEVPSPEVVALVLEEMWKFNQTYQLAHGTAEEKVPVW-----------------YIM 206

Query: 482 DELGSALRHSDEPNFRVAPFLFMPEGELTSAISFTILWPIHNVQKGDECTRDYLFGFGED 541
           DE GS ++HSD P+F  APF +MP+      +++T+LWP+ ++  G+E TRD+ +G  + 
Sbjct: 207 DEFGSRIQHSDMPSFATAPFFYMPQ-----QVAYTLLWPLRDLDTGEEVTRDFAYGEADP 266

Query: 542 KQRSARLTAW---------FHTPQNYFVHEYEKHIRNLQSKALASSISQTTSNPEGLHQS 601
             R   L  W         F TP+     +Y + I     + L  +IS          + 
Sbjct: 267 LIRKCMLLPWAPADMLDLSFSTPEP--PAKYYQAILEENKEKLPLAISPVA-------RP 326

Query: 602 KGGTFHVYTDNPQVEEFLNRPEFIMTSDPKEADIIWTSMQIDEDTRKATGITDQQYVNQF 661
           +G  F V+ D  QV   L  P F  T    +ADI +      +D  K +  + Q  +NQF
Sbjct: 327 QGHVFRVHCDVQQVLGHLTHPRFTFTDSEADADIFFHFSHF-KDYMKLSQESPQVLLNQF 386

Query: 662 PFEACLVMKHHLAETVEKAHG--CPEWLQPTYNLETHLSQLIGDYLVRKRDGLNNLWILK 721
           P E  L +K  LA    +A G   P WL  T+NL T L Q +  +  R+R G +N WI K
Sbjct: 387 PCENLLTVKDCLASIARRAGGPEGPPWLPRTFNLRTELPQFVSYFQHRERRGEDNHWICK 446

Query: 722 PWNMARTIDTTVTDNLSAIIRLMETGPKICQKYIEHPALF-----NGKKFDLRYIVLVRS 781
           PWN+AR++DT VT+NL +IIR  E+ PK+  KYIE P LF        KFD+RYIVL+RS
Sbjct: 447 PWNLARSLDTHVTNNLHSIIRHRESTPKVVSKYIESPVLFLREDVGNVKFDIRYIVLLRS 506

Query: 782 MKPLEIFLADSFWVRLANNPYSLETQSLFEYETHFTVMFYLAVSVACNSSWCAREIDYN- 841
           ++PL +F  D FW+R +N P++L+   L +YE HFTVM Y       +     +++ YN 
Sbjct: 507 VRPLRLFAYDVFWLRFSNRPFALD--DLDDYEKHFTVMNY-------DPDVVLKQVHYNE 566

Query: 842 ------------AWLDIHSRVRSMIRSVFESAMVVHPEM---HSPFSRAIYGLDVMLD-- 901
                        W D+ + +      +F+ A    P M     P SRA+Y +D+ML+  
Sbjct: 567 FIPQFEKQYPEFPWSDVQAEIFKAFTELFQVACAKPPPMGLCDYPSSRAMYAIDLMLNWD 626

Query: 902 ------SSFQPKLLEVTYCPDCTRACKYDVENVYGGVIKGKEFYNDVFGCLFLNET--TH 919
                    QP++LEV + PDC RAC+Y              F+NDVF  LFL+ET   H
Sbjct: 627 NHPDGKRVMQPQILEVNFNPDCERACRYH-----------PSFFNDVFSTLFLDETDNCH 636


HSP 2 Score: 54.7 bits (130), Expect = 6.5e-06
Identity = 34/101 (33.66%), Postives = 50/101 (49.50%), Query Frame = 0

Query: 63  LCTKLTSETFDGGAHF---QVEPFEDGR------------------CRRLVLSSDFMAK- 122
           L  KL  E FD G  F   QVE  E+                    C +++++S+   + 
Sbjct: 47  LLHKLEHEVFDAGEMFGIMQVEEVEEAEDEAAREAQRKQPNPGGELCYKVIVTSESGVRA 106

Query: 123 --ESHVFLVDHAWTFRLSDAYTQLLEVPGLAERMASLMCVD 140
              + +FL+DHAWT R+  A  QL +VPGL  RMA+LM ++
Sbjct: 107 DDPNSIFLIDHAWTCRVEHARKQLQQVPGLLHRMANLMGIE 147

BLAST of Lsi06G003350 vs. ExPASy Swiss-Prot
Match: Q14166 (Tubulin--tyrosine ligase-like protein 12 OS=Homo sapiens OX=9606 GN=TTLL12 PE=1 SV=2)

HSP 1 Score: 278.5 bits (711), Expect = 2.8e-73
Identity = 178/514 (34.63%), Postives = 256/514 (49.81%), Query Frame = 0

Query: 433 VINAMWQYLMTYRLADEEKIDETSVWYAVYRLGLSYFSIFSRMYVMDELGSALRHSDEPN 492
           V+  MW++  TY+LA     ++  VW                 Y+MDE GS ++H+D P+
Sbjct: 166 VLEEMWKFNQTYQLAHGTAEEKMPVW-----------------YIMDEFGSRIQHADVPS 225

Query: 493 FRVAPFLFMPEGELTSAISFTILWPIHNVQKGDECTRDYLFGFGEDKQRSARLTAWFHTP 552
           F  APF +MP+      +++T+LWP+ ++  G+E TRD+ +G  +   R   L  W  T 
Sbjct: 226 FATAPFFYMPQ-----QVAYTLLWPLRDLDTGEEVTRDFAYGETDPLIRKCMLLPWAPTD 285

Query: 553 QNYFVHEYEKHIRNLQSKALASSISQTTSNPEGLHQSKGGTFHVYTDNPQVEEFLNRPEF 612
                    +         L  +  +   +   +    G  F VYTD  QV   L  P F
Sbjct: 286 MLDLSSCTPEPPAEHYQAILEENKEKLPLDINPVVHPHGHIFKVYTDVQQVASSLTHPRF 345

Query: 613 IMTSDPKEADIIWTSMQIDEDTRKATGITDQQYVNQFPFEACLVMKHHLAETVEKAHG-- 672
            +T    +ADI++      +D RK +       +NQFP E  L +K  LA    +A G  
Sbjct: 346 TLTQSEADADILFNFSHF-KDYRKLSQERPGVLLNQFPCENLLTVKDCLASIARRAGGPE 405

Query: 673 CPEWLQPTYNLETHLSQLIGDYLVRKRDGLNNLWILKPWNMARTIDTTVTDNLSAIIRLM 732
            P WL  T+NL T L Q +  +  R+R G +N WI KPWN+AR++DT VT +L +IIR  
Sbjct: 406 GPPWLPRTFNLRTELPQFVSYFQQRERWGEDNHWICKPWNLARSLDTHVTKSLHSIIRHR 465

Query: 733 ETGPKICQKYIEHPALF----NGK-KFDLRYIVLVRSMKPLEIFLADSFWVRLANNPYSL 792
           E+ PK+  KYIE P LF     GK KFD+RYIVL+RS++PL +F+ D FW+R +N  ++L
Sbjct: 466 ESTPKVVSKYIESPVLFLREDVGKVKFDIRYIVLLRSVRPLRLFVYDVFWLRFSNRAFAL 525

Query: 793 ETQSLFEYETHFTVMFY----LAVSVACNSSWCAREIDYN--AWLDIHSRVRSMIRSVFE 852
               L +YE HFTVM Y    +   V C       E  Y    W D+ + +      +F+
Sbjct: 526 --NDLDDYEKHFTVMNYDPDVVLKQVHCEEFIPEFEKQYPEFPWTDVQAEIFRAFTELFQ 585

Query: 853 SAMVVHPEM---HSPFSRAIYGLDVMLD--------SSFQPKLLEVTYCPDCTRACKYDV 912
            A    P +     P SRA+Y +D+ML            QP++LEV + PDC RAC+Y  
Sbjct: 586 VACAKPPPLGLCDYPSSRAMYAVDLMLKWDNGPDGRRVMQPQILEVNFNPDCERACRYH- 643

Query: 913 ENVYGGVIKGKEFYNDVFGCLFLNET--THVTPL 921
                       F+NDVF  LFL++    HVT L
Sbjct: 646 ----------PTFFNDVFSTLFLDQPGGCHVTCL 643


HSP 2 Score: 51.6 bits (122), Expect = 5.5e-05
Identity = 43/144 (29.86%), Postives = 63/144 (43.75%), Query Frame = 0

Query: 63  LCTKLTSETFDGGAHF---QVEPFEDGR------------------CRRLVLSSDFMAKE 122
           L  KL  E FD G  F   QVE  E+                    C +++++ +   + 
Sbjct: 52  LLHKLEHEVFDAGEVFGIMQVEEVEEEEDEAAREVRKQQPNPGNELCYKVIVTRESGLQA 111

Query: 123 SH---VFLVDHAWTFRLSDAYTQLLEVPGLAERMASLMCVDVDMNLDEEE------EDQS 169
           +H   +FL+DHAWT R+  A  QL +VPGL  RMA+LM ++    L   E      E+  
Sbjct: 112 AHPNSIFLIDHAWTCRVEHARQQLQQVPGLLHRMANLMGIEFHGELPSTEAVALVLEEMW 171

BLAST of Lsi06G003350 vs. ExPASy Swiss-Prot
Match: Q09512 (Tubulin--tyrosine ligase-like protein 12 OS=Caenorhabditis elegans OX=6239 GN=ttll-12 PE=3 SV=1)

HSP 1 Score: 239.6 bits (610), Expect = 1.4e-61
Identity = 167/536 (31.16%), Postives = 258/536 (48.13%), Query Frame = 0

Query: 425 PEEPLPDRVINAMWQYLMTYRLA---DEEKIDETSVWYAVYRLGLSYFSIFSRMYVMDEL 484
           P+  L ++V+ A+W+Y  TY ++   D  +I++ SVW                 YVMD+ 
Sbjct: 168 PKNELTEKVLKALWKYSQTYSVSYQLDNGEIEKKSVW-----------------YVMDDF 227

Query: 485 GSALRHSDEPNFRVAPFLFMPEGELTSAISFTILWPIHNVQKGDECTRDYLFGFGEDKQR 544
           G+ +RHS EPN R+ P +F+P+       +++I++    V   +E   D+          
Sbjct: 228 GTRVRHSTEPNVRIVPLMFLPQN-----CAYSIMFLTKPVNTDEEIMMDW-----ASNVI 287

Query: 545 SARLTAW-FHTPQNYFVHEYEKH------------IRNLQSKALASSISQTTSNP---EG 604
           +A+   W  +  Q +   ++ K                     LA    Q T        
Sbjct: 288 TAQHPEWRKYIEQPWAAQDFSKETMIPDAPTLEYFTSGRNPDFLAGPTEQQTCQSAIFTS 347

Query: 605 LHQSKGGTFHVYTDNPQVEEFLNRPEFIMTSDPKEADIIWTSMQIDEDTRKATGITDQQY 664
           L   K     VY D+ Q+ E L   +     D K+AD+IW       D ++ +       
Sbjct: 348 LPILKKRKIKVYADDTQLTEHLKNHKVEYVDDIKKADVIWMIKHF-HDYKQLSEENPCGM 407

Query: 665 VNQFPFEACLVMKHHLAETVEKAHGCPEWLQPTYNLETHLSQLIGDYLVRKRDGLNNLWI 724
           +NQFPFE+C+ +K  LA    +     +W Q TYNL T L + +  +  R+ +G +N+WI
Sbjct: 408 INQFPFESCITVKDLLAACAMRDPAKNDWYQLTYNLNTQLPEFVARFQNRELNGQHNVWI 467

Query: 725 LKPWNMARTIDTTVTDNLSAIIRLMETGPKICQKYIEHPALF------NGKKFDLRYIVL 784
           +KPWN+AR +D TVT++L+ IIR++ETGPKI  +YI  P LF      N  KFDLRYIV 
Sbjct: 468 VKPWNLARGMDMTVTEDLNQIIRMIETGPKIVCEYIPRPLLFPRPDNGNKVKFDLRYIVF 527

Query: 785 VRSMKPLEIFLADSFWVRLANNPYSLETQSLFEYETHFTVMFYL----AVSVACNSSWCA 844
           +  + P+  ++ + FW+R A N +SL   +  + ETHFTV  YL     + + C +    
Sbjct: 528 LNGIAPVTAYVYNRFWIRFAINEFSL--SNFEDVETHFTVFNYLDKEKILQMKCENFIET 587

Query: 845 REIDYN--AWLDIHSRVRSMIRSVFESAMVVH-PEMHSP--FSRAIYGLDVML----DSS 904
            E  Y    W ++   +   IR   E+A     P   +P   SRA+YG+D+ML    +  
Sbjct: 588 IEKAYPRIQWSEVQKDINLTIRKAIEAAAKEEAPRGVAPNVQSRAMYGVDIMLQHGDNDV 647

Query: 905 FQPKLLEVTYCPDCTRACKYDVENVYGGVIKGKEFYNDVFGCLFLNE--TTHVTPL 921
            +  LLE+ + PD TRAC+Y             +F + VF  LFL+E   T VTP+
Sbjct: 648 IKSTLLEINFMPDTTRACQY-----------YPDFADTVFETLFLDEIDPTKVTPI 662

BLAST of Lsi06G003350 vs. ExPASy Swiss-Prot
Match: A8XXC0 (Tubulin--tyrosine ligase-like protein 12 OS=Caenorhabditis briggsae OX=6238 GN=ttll-12 PE=3 SV=2)

HSP 1 Score: 235.3 bits (599), Expect = 2.7e-60
Identity = 169/540 (31.30%), Postives = 263/540 (48.70%), Query Frame = 0

Query: 425 PEEPLPDRVINAMWQYLMTYRLA---DEEKIDETSVWYAVYRLGLSYFSIFSRMYVMDEL 484
           P++ + +RV+  +W++  TY ++   +  ++++  VW                 YVMD+ 
Sbjct: 171 PKKTMTERVMRNLWKFAQTYTVSYQLENGEMEKKHVW-----------------YVMDDF 230

Query: 485 GSALRHSDEPNFRVAPFLFMPEG------ELTSAI----SFTILWPIHNVQKGDECTRDY 544
           GS +RHS  PN R+ P +F+P+        LT  +      T+ W  + +   +   R Y
Sbjct: 231 GSRIRHSGCPNVRIVPLMFLPQNCAYSIMFLTKPVKIDDEITMDWAANVITAKNPEWRQY 290

Query: 545 LFGFGEDKQRSAR-------LTAWFHTPQNYFVHEYEKHIRNLQSKALASSISQTTSNPE 604
           L     +K  S+           +F + +N      EK  +  +S A+ S++S       
Sbjct: 291 LEMPWAEKDFSSESMVPGPPTLEYFTSGRNPDFLADEKDKKTCES-AIFSALSVLKK--- 350

Query: 605 GLHQSKGGTF-HVYTDNPQVEEFLNRPEFIMTSDPKEADIIWTSMQIDEDTRKATGITDQ 664
              Q K   F  ++ D+ Q+ E L   +     D K AD+IW      + +  A      
Sbjct: 351 ---QGKIKIFLQIFADDTQLTEHLKSRQIEYVDDWKAADVIWMIKHFHDYSNLAQENPCA 410

Query: 665 QYVNQFPFEACLVMKHHLAETVEKAHGCPEWLQPTYNLETHLSQLIGDYLVRKRDGLNNL 724
           Q +NQFPFE+C+ +K  LA    +     +W Q TYNL T L + +  +  R+++G +N+
Sbjct: 411 Q-INQFPFESCITVKDLLAACAMRDPSKNDWYQLTYNLNTQLPEFVARFQNRQKNGEHNV 470

Query: 725 WILKPWNMARTIDTTVTDNLSAIIRLMETGPKICQKYIEHPALF------NGKKFDLRYI 784
           WI+KPWN+AR ++  VTD+L+ IIR++ETGPKI  +YI  P LF      N  KFDLRYI
Sbjct: 471 WIVKPWNLARGMEMAVTDDLNQIIRMVETGPKIVCEYIARPLLFPRPDNGNKVKFDLRYI 530

Query: 785 VLVRSMKPLEIFLADSFWVRLANNPYSLETQSLFEYETHFTVMFYL----AVSVACNSSW 844
           V V ++ P+  ++ + FW+R A N + L      + ETHFTV  YL     + + C    
Sbjct: 531 VFVNAIGPVTAYVYNRFWIRFAINQFQLGAYD--DLETHFTVFNYLDKEKVLQMKCEKFV 590

Query: 845 CAREIDYN--AWLDIHSRVRSMIRSVFE-SAMVVHPEMHSP--FSRAIYGLDVMLDSS-- 904
              E  Y    W  I + + S IR   E +A    P   +P   SRA+YG+D+ML  S  
Sbjct: 591 EIIEKTYPKLKWTQIQADINSTIRKAIEVAASEPSPRGVAPNTQSRAMYGVDIMLQESPE 650

Query: 905 ----FQPKLLEVTYCPDCTRACKYDVENVYGGVIKGKEFYNDVFGCLFLNE--TTHVTPL 921
                +P LLE+ + PD TRAC+Y             +F + VF  +FL+E   T VTP+
Sbjct: 651 SPDVIKPTLLEINFMPDTTRACQY-----------YPDFADTVFDTMFLDEIDPTKVTPI 672

BLAST of Lsi06G003350 vs. ExPASy Swiss-Prot
Match: Q9VM91 (Tubulin glycylase 3A OS=Drosophila melanogaster OX=7227 GN=TTLL3A PE=1 SV=1)

HSP 1 Score: 72.4 bits (176), Expect = 3.0e-11
Identity = 58/226 (25.66%), Postives = 95/226 (42.04%), Query Frame = 0

Query: 698 DGLNNLWILKPWNMARTIDTTVTDNLSAI-----IRLMETGPKICQKYIEHPALFNGKKF 757
           DG  N+WI+KP N  R     + DNL  I     + +      + QKYIE P +    KF
Sbjct: 412 DGYQNMWIVKPANKCRGRGIILMDNLKKILGVVNLSIASKSRYVVQKYIERPLILFQTKF 471

Query: 758 DLRYIVLVRSMKPLEIFLADSFWVRLANNPYSLETQSLFEYETHFTVMFYLA-----VSV 817
           D+R   L+ + +PL ++     ++R ++  YSL       + T++ +            +
Sbjct: 472 DIRQWFLITNTQPLVVWFYRESYLRFSSQEYSLSNHHESVHLTNYAIQKKYTNGKRDKRL 531

Query: 818 ACNSSW-------CAREI-DYNAWLDIHSRVRSMIRSVFESAMVVHPEM--HSPFSRAIY 877
              + W         R+I  YN WL+   R+   +R      M+   E     P +  ++
Sbjct: 532 PSENMWDCYSFQAYLRQIGKYNMWLE---RIFPGMRKAIVGCMLASQENMDRRPNTFELF 591

Query: 878 GLDVMLDSSFQPKLLEVTYCPD-------CTRACKYDVENVYGGVI 897
           G D M+  +F P L+E+   PD         R C   +E+V   VI
Sbjct: 592 GADFMICENFYPWLIEINSSPDLGATTSVTARMCPQCLEDVVKVVI 634

BLAST of Lsi06G003350 vs. ExPASy TrEMBL
Match: A0A5D3CI02 (Tubulin--tyrosine ligase-like protein 12 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold16G001580 PE=4 SV=1)

HSP 1 Score: 1530.4 bits (3961), Expect = 0.0e+00
Identity = 758/883 (85.84%), Postives = 791/883 (89.58%), Query Frame = 0

Query: 45  RISSKFMVCSSLPLDF-PSLCTKLTSETFDGGAHFQVEPFEDGRCRRLVLSSDFMAKESH 104
           ++    +  S LP      L  KLTSETFDGG+HFQVEPFEDGRCRRLVLSSDFMAKESH
Sbjct: 15  KVHGLLLTASGLPQSLHRQLFQKLTSETFDGGSHFQVEPFEDGRCRRLVLSSDFMAKESH 74

Query: 105 VFLVDHAWTFRLSDAYTQLLEVPGLAERMASLMCVDVDMNLDEEEEDQSKSN--DDEAKQ 164
           VFLVDHAWTFRLSDAY QLLEVPGLAERMASLMCVD+D+NLDEE+ED SKSN  DD+AKQ
Sbjct: 75  VFLVDHAWTFRLSDAYKQLLEVPGLAERMASLMCVDIDLNLDEEDEDHSKSNVDDDDAKQ 134

Query: 165 SVWELLESEIRGAKEKGNDSVRWLELEDLQIEDDTLLSLDLPTKFPDLLALSLTGNKLRN 224
           SVWEL+ESEIRGA EKGNDSVRWLELEDLQI+DDTLLSLDLPTKFPDLLALSLTGNKL++
Sbjct: 135 SVWELIESEIRGAIEKGNDSVRWLELEDLQIDDDTLLSLDLPTKFPDLLALSLTGNKLKD 194

Query: 225 VDVIAQEVAKFKHLRALWLNDNPVAENWYFSELFLFSDENLQQKVLEGSPNLEIYNSRFT 284
           VD +A+EV KFKHLRALWLNDNPVAEN          D +LQQ+VLEGSPNLEIYNSRFT
Sbjct: 195 VDTVAREVVKFKHLRALWLNDNPVAEN---------CDASLQQRVLEGSPNLEIYNSRFT 254

Query: 285 LNFSKWALGFCGDMYGKDNPGSIYPADHTLQCLTSLDLSSRYIHNLINKAFSPVELPSLL 344
           LNFSKWALGFCGDMYGKDNPGSIYP+DHTLQCLTSLDLSSR IHNLINKAFSPVELPSL 
Sbjct: 255 LNFSKWALGFCGDMYGKDNPGSIYPSDHTLQCLTSLDLSSRCIHNLINKAFSPVELPSLS 314

Query: 345 YLNLKGNPLEQNSVGDLLKVLKEFPCLSSLEVDIPGPLGEKASDIIESLPNLNALNGIDV 404
           YLNL+GNPLEQNSVGDLLK+LKEFPCLSSLEVDIPGPLGEKASDIIESLPNL+ LNGIDV
Sbjct: 315 YLNLRGNPLEQNSVGDLLKILKEFPCLSSLEVDIPGPLGEKASDIIESLPNLSNLNGIDV 374

Query: 405 AKILNSGKHVIDSMLLPRLPEWAPEEPLPDRVINAMWQYLMTYRLADEEKIDETSVWYAV 464
            KIL+SGKH+IDSMLLPRLPEWAPEE LPDRVINAMWQYLMTYRLADEEKIDETSVW   
Sbjct: 375 EKILSSGKHMIDSMLLPRLPEWAPEETLPDRVINAMWQYLMTYRLADEEKIDETSVW--- 434

Query: 465 YRLGLSYFSIFSRMYVMDELGSALRHSDEPNFRVAPFLFMPEGELTSAISFTILWPIHNV 524
                         YVMDELGSALRHSDEPNFRVAPFLFMPEG LTSAISFTILWPIHNV
Sbjct: 435 --------------YVMDELGSALRHSDEPNFRVAPFLFMPEGNLTSAISFTILWPIHNV 494

Query: 525 QKGDECTRDYLFGFGEDKQRSARLTAWFHTPQNYFVHEYEKHIRNLQSKALASSISQTTS 584
           QKGDECTRDYLFG GEDKQRSARLTAWFHTPQNYFVHEYEKHI+NLQSK L S ISQTTS
Sbjct: 495 QKGDECTRDYLFGIGEDKQRSARLTAWFHTPQNYFVHEYEKHIKNLQSKVLTSPISQTTS 554

Query: 585 NPEGLHQSKGGTFHVYTDNPQVEEFLNRPEFIMTSDPKEADIIWTSMQIDEDTRKATGIT 644
             E L QSKGGT  VYTDNPQVEEFLNRPEF +TSDPKEADIIWTSMQIDEDTRKATGIT
Sbjct: 555 KTEELRQSKGGTLRVYTDNPQVEEFLNRPEFTITSDPKEADIIWTSMQIDEDTRKATGIT 614

Query: 645 DQQYVNQFPFEACLVMKHHLAETVEKAHGCPEWLQPTYNLETHLSQLIGDYLVRKRDGLN 704
           D+QYVNQFPFEACLVMKHHLAET+EKAHGCPEWLQPTYNLETHLSQLIGDY VRKRDGLN
Sbjct: 615 DKQYVNQFPFEACLVMKHHLAETIEKAHGCPEWLQPTYNLETHLSQLIGDYFVRKRDGLN 674

Query: 705 NLWILKPWNMARTIDTTVTDNLSAIIRLMETGPKICQKYIEHPALFNGKKFDLRYIVLVR 764
           NLWILKPWNMARTIDTTVTDNLSAIIRLMETGPKICQKYIEHPALFNGKKFDLRYIVLVR
Sbjct: 675 NLWILKPWNMARTIDTTVTDNLSAIIRLMETGPKICQKYIEHPALFNGKKFDLRYIVLVR 734

Query: 765 SMKPLEIFLADSFWVRLANNPYSLETQSLFEYETHFTVMFYLAVSVACNSSWCAREIDYN 824
           SMKPLEIFLADSFWVRLANNPYSLE QSLFEYETHFTVM Y       N +   RE +  
Sbjct: 735 SMKPLEIFLADSFWVRLANNPYSLEKQSLFEYETHFTVMNYRGRLNHKNIADFVREFEQE 794

Query: 825 ---AWLDIHSRVRSMIRSVFESAMVVHPEMHSPFSRAIYGLDVMLDSSFQPKLLEVTYCP 884
               WLDIHSRV SMIRSVFESA VVHPEMHSPFSRA+YGLDVMLDSSFQPKLLEVTYCP
Sbjct: 795 HNVKWLDIHSRVTSMIRSVFESAAVVHPEMHSPFSRAMYGLDVMLDSSFQPKLLEVTYCP 854

Query: 885 DCTRACKYDVENVY-GGVIKGKEFYNDVFGCLFLNETTHVTPL 921
           DCTRACKYDVENV+ GGVI+G+EFYN +FGCLFLNETTHVTPL
Sbjct: 855 DCTRACKYDVENVFGGGVIEGREFYNYIFGCLFLNETTHVTPL 871

BLAST of Lsi06G003350 vs. ExPASy TrEMBL
Match: A0A5A7UQ89 (Tubulin--tyrosine ligase-like protein 12 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold181G001610 PE=4 SV=1)

HSP 1 Score: 1528.5 bits (3956), Expect = 0.0e+00
Identity = 757/883 (85.73%), Postives = 791/883 (89.58%), Query Frame = 0

Query: 45  RISSKFMVCSSLPLDF-PSLCTKLTSETFDGGAHFQVEPFEDGRCRRLVLSSDFMAKESH 104
           ++    +  S LP      L  KLTSETFDGG+HFQVEPFEDGRCRRLVLSSDFMAKESH
Sbjct: 15  KVHGLLLTASGLPQSLHRQLFQKLTSETFDGGSHFQVEPFEDGRCRRLVLSSDFMAKESH 74

Query: 105 VFLVDHAWTFRLSDAYTQLLEVPGLAERMASLMCVDVDMNLDEEEEDQSKSN--DDEAKQ 164
           VFLVDHAWTFRLSDAY QLLEVPGLAERMASLMCVD+D+NLDEE+ED SKSN  DD+AKQ
Sbjct: 75  VFLVDHAWTFRLSDAYKQLLEVPGLAERMASLMCVDIDLNLDEEDEDHSKSNVDDDDAKQ 134

Query: 165 SVWELLESEIRGAKEKGNDSVRWLELEDLQIEDDTLLSLDLPTKFPDLLALSLTGNKLRN 224
           SVWEL+ESEIRGA EKGNDSVRWLELE+LQI+DDTLLSLDLPTKFPDLLALSLTGNKL++
Sbjct: 135 SVWELIESEIRGAIEKGNDSVRWLELENLQIDDDTLLSLDLPTKFPDLLALSLTGNKLKD 194

Query: 225 VDVIAQEVAKFKHLRALWLNDNPVAENWYFSELFLFSDENLQQKVLEGSPNLEIYNSRFT 284
           VD +A+EV KFKHLRALWLNDNPVAEN          D +LQQ+VLEGSPNLEIYNSRFT
Sbjct: 195 VDTVAREVVKFKHLRALWLNDNPVAEN---------CDASLQQRVLEGSPNLEIYNSRFT 254

Query: 285 LNFSKWALGFCGDMYGKDNPGSIYPADHTLQCLTSLDLSSRYIHNLINKAFSPVELPSLL 344
           LNFSKWALGFCGDMYGKDNPGSIYP+DHTLQCLTSLDLSSR IHNLINKAFSPVELPSL 
Sbjct: 255 LNFSKWALGFCGDMYGKDNPGSIYPSDHTLQCLTSLDLSSRCIHNLINKAFSPVELPSLS 314

Query: 345 YLNLKGNPLEQNSVGDLLKVLKEFPCLSSLEVDIPGPLGEKASDIIESLPNLNALNGIDV 404
           YLNL+GNPLEQNSVGDLLK+LKEFPCLSSLEVDIPGPLGEKASDIIESLPNL+ LNGIDV
Sbjct: 315 YLNLRGNPLEQNSVGDLLKILKEFPCLSSLEVDIPGPLGEKASDIIESLPNLSNLNGIDV 374

Query: 405 AKILNSGKHVIDSMLLPRLPEWAPEEPLPDRVINAMWQYLMTYRLADEEKIDETSVWYAV 464
            KIL+SGKH+IDSMLLPRLPEWAPEE LPDRVINAMWQYLMTYRLADEEKIDETSVW   
Sbjct: 375 EKILSSGKHMIDSMLLPRLPEWAPEETLPDRVINAMWQYLMTYRLADEEKIDETSVW--- 434

Query: 465 YRLGLSYFSIFSRMYVMDELGSALRHSDEPNFRVAPFLFMPEGELTSAISFTILWPIHNV 524
                         YVMDELGSALRHSDEPNFRVAPFLFMPEG LTSAISFTILWPIHNV
Sbjct: 435 --------------YVMDELGSALRHSDEPNFRVAPFLFMPEGNLTSAISFTILWPIHNV 494

Query: 525 QKGDECTRDYLFGFGEDKQRSARLTAWFHTPQNYFVHEYEKHIRNLQSKALASSISQTTS 584
           QKGDECTRDYLFG GEDKQRSARLTAWFHTPQNYFVHEYEKHI+NLQSK L S ISQTTS
Sbjct: 495 QKGDECTRDYLFGIGEDKQRSARLTAWFHTPQNYFVHEYEKHIKNLQSKVLTSPISQTTS 554

Query: 585 NPEGLHQSKGGTFHVYTDNPQVEEFLNRPEFIMTSDPKEADIIWTSMQIDEDTRKATGIT 644
             E L QSKGGT  VYTDNPQVEEFLNRPEF +TSDPKEADIIWTSMQIDEDTRKATGIT
Sbjct: 555 KTEELRQSKGGTLRVYTDNPQVEEFLNRPEFTITSDPKEADIIWTSMQIDEDTRKATGIT 614

Query: 645 DQQYVNQFPFEACLVMKHHLAETVEKAHGCPEWLQPTYNLETHLSQLIGDYLVRKRDGLN 704
           D+QYVNQFPFEACLVMKHHLAET+EKAHGCPEWLQPTYNLETHLSQLIGDY VRKRDGLN
Sbjct: 615 DKQYVNQFPFEACLVMKHHLAETIEKAHGCPEWLQPTYNLETHLSQLIGDYFVRKRDGLN 674

Query: 705 NLWILKPWNMARTIDTTVTDNLSAIIRLMETGPKICQKYIEHPALFNGKKFDLRYIVLVR 764
           NLWILKPWNMARTIDTTVTDNLSAIIRLMETGPKICQKYIEHPALFNGKKFDLRYIVLVR
Sbjct: 675 NLWILKPWNMARTIDTTVTDNLSAIIRLMETGPKICQKYIEHPALFNGKKFDLRYIVLVR 734

Query: 765 SMKPLEIFLADSFWVRLANNPYSLETQSLFEYETHFTVMFYLAVSVACNSSWCAREIDYN 824
           SMKPLEIFLADSFWVRLANNPYSLE QSLFEYETHFTVM Y       N +   RE +  
Sbjct: 735 SMKPLEIFLADSFWVRLANNPYSLEKQSLFEYETHFTVMNYRGRLNHKNIADFVREFEQE 794

Query: 825 ---AWLDIHSRVRSMIRSVFESAMVVHPEMHSPFSRAIYGLDVMLDSSFQPKLLEVTYCP 884
               WLDIHSRV SMIRSVFESA VVHPEMHSPFSRA+YGLDVMLDSSFQPKLLEVTYCP
Sbjct: 795 HNVKWLDIHSRVTSMIRSVFESAAVVHPEMHSPFSRAMYGLDVMLDSSFQPKLLEVTYCP 854

Query: 885 DCTRACKYDVENVY-GGVIKGKEFYNDVFGCLFLNETTHVTPL 921
           DCTRACKYDVENV+ GGVI+G+EFYN +FGCLFLNETTHVTPL
Sbjct: 855 DCTRACKYDVENVFGGGVIEGREFYNYIFGCLFLNETTHVTPL 871

BLAST of Lsi06G003350 vs. ExPASy TrEMBL
Match: A0A1S3BR24 (tubulin--tyrosine ligase-like protein 12 OS=Cucumis melo OX=3656 GN=LOC103492368 PE=4 SV=1)

HSP 1 Score: 1528.5 bits (3956), Expect = 0.0e+00
Identity = 757/883 (85.73%), Postives = 791/883 (89.58%), Query Frame = 0

Query: 45  RISSKFMVCSSLPLDF-PSLCTKLTSETFDGGAHFQVEPFEDGRCRRLVLSSDFMAKESH 104
           ++    +  S LP      L  KLTSETFDGG+HFQVEPFEDGRCRRLVLSSDFMAKESH
Sbjct: 15  KVHGLLLTASGLPQSLHRQLFQKLTSETFDGGSHFQVEPFEDGRCRRLVLSSDFMAKESH 74

Query: 105 VFLVDHAWTFRLSDAYTQLLEVPGLAERMASLMCVDVDMNLDEEEEDQSKSN--DDEAKQ 164
           VFLVDHAWTFRLSDAY QLLEVPGLAERMASLMCVD+D+NLDEE+ED SKSN  DD+AKQ
Sbjct: 75  VFLVDHAWTFRLSDAYKQLLEVPGLAERMASLMCVDIDLNLDEEDEDHSKSNVDDDDAKQ 134

Query: 165 SVWELLESEIRGAKEKGNDSVRWLELEDLQIEDDTLLSLDLPTKFPDLLALSLTGNKLRN 224
           SVWEL+ESEIRGA EKGNDSVRWLELE+LQI+DDTLLSLDLPTKFPDLLALSLTGNKL++
Sbjct: 135 SVWELIESEIRGAIEKGNDSVRWLELENLQIDDDTLLSLDLPTKFPDLLALSLTGNKLKD 194

Query: 225 VDVIAQEVAKFKHLRALWLNDNPVAENWYFSELFLFSDENLQQKVLEGSPNLEIYNSRFT 284
           VD +A+EV KFKHLRALWLNDNPVAEN          D +LQQ+VLEGSPNLEIYNSRFT
Sbjct: 195 VDTVAREVVKFKHLRALWLNDNPVAEN---------CDASLQQRVLEGSPNLEIYNSRFT 254

Query: 285 LNFSKWALGFCGDMYGKDNPGSIYPADHTLQCLTSLDLSSRYIHNLINKAFSPVELPSLL 344
           LNFSKWALGFCGDMYGKDNPGSIYP+DHTLQCLTSLDLSSR IHNLINKAFSPVELPSL 
Sbjct: 255 LNFSKWALGFCGDMYGKDNPGSIYPSDHTLQCLTSLDLSSRCIHNLINKAFSPVELPSLS 314

Query: 345 YLNLKGNPLEQNSVGDLLKVLKEFPCLSSLEVDIPGPLGEKASDIIESLPNLNALNGIDV 404
           YLNL+GNPLEQNSVGDLLK+LKEFPCLSSLEVDIPGPLGEKASDIIESLPNL+ LNGIDV
Sbjct: 315 YLNLRGNPLEQNSVGDLLKILKEFPCLSSLEVDIPGPLGEKASDIIESLPNLSNLNGIDV 374

Query: 405 AKILNSGKHVIDSMLLPRLPEWAPEEPLPDRVINAMWQYLMTYRLADEEKIDETSVWYAV 464
            KIL+SGKH+IDSMLLPRLPEWAPEE LPDRVINAMWQYLMTYRLADEEKIDETSVW   
Sbjct: 375 EKILSSGKHMIDSMLLPRLPEWAPEETLPDRVINAMWQYLMTYRLADEEKIDETSVW--- 434

Query: 465 YRLGLSYFSIFSRMYVMDELGSALRHSDEPNFRVAPFLFMPEGELTSAISFTILWPIHNV 524
                         YVMDELGSALRHSDEPNFRVAPFLFMPEG LTSAISFTILWPIHNV
Sbjct: 435 --------------YVMDELGSALRHSDEPNFRVAPFLFMPEGNLTSAISFTILWPIHNV 494

Query: 525 QKGDECTRDYLFGFGEDKQRSARLTAWFHTPQNYFVHEYEKHIRNLQSKALASSISQTTS 584
           QKGDECTRDYLFG GEDKQRSARLTAWFHTPQNYFVHEYEKHI+NLQSK L S ISQTTS
Sbjct: 495 QKGDECTRDYLFGIGEDKQRSARLTAWFHTPQNYFVHEYEKHIKNLQSKVLTSPISQTTS 554

Query: 585 NPEGLHQSKGGTFHVYTDNPQVEEFLNRPEFIMTSDPKEADIIWTSMQIDEDTRKATGIT 644
             E L QSKGGT  VYTDNPQVEEFLNRPEF +TSDPKEADIIWTSMQIDEDTRKATGIT
Sbjct: 555 KTEELRQSKGGTLRVYTDNPQVEEFLNRPEFTITSDPKEADIIWTSMQIDEDTRKATGIT 614

Query: 645 DQQYVNQFPFEACLVMKHHLAETVEKAHGCPEWLQPTYNLETHLSQLIGDYLVRKRDGLN 704
           D+QYVNQFPFEACLVMKHHLAET+EKAHGCPEWLQPTYNLETHLSQLIGDY VRKRDGLN
Sbjct: 615 DKQYVNQFPFEACLVMKHHLAETIEKAHGCPEWLQPTYNLETHLSQLIGDYFVRKRDGLN 674

Query: 705 NLWILKPWNMARTIDTTVTDNLSAIIRLMETGPKICQKYIEHPALFNGKKFDLRYIVLVR 764
           NLWILKPWNMARTIDTTVTDNLSAIIRLMETGPKICQKYIEHPALFNGKKFDLRYIVLVR
Sbjct: 675 NLWILKPWNMARTIDTTVTDNLSAIIRLMETGPKICQKYIEHPALFNGKKFDLRYIVLVR 734

Query: 765 SMKPLEIFLADSFWVRLANNPYSLETQSLFEYETHFTVMFYLAVSVACNSSWCAREIDYN 824
           SMKPLEIFLADSFWVRLANNPYSLE QSLFEYETHFTVM Y       N +   RE +  
Sbjct: 735 SMKPLEIFLADSFWVRLANNPYSLEKQSLFEYETHFTVMNYRGRLNHKNIADFVREFEQE 794

Query: 825 ---AWLDIHSRVRSMIRSVFESAMVVHPEMHSPFSRAIYGLDVMLDSSFQPKLLEVTYCP 884
               WLDIHSRV SMIRSVFESA VVHPEMHSPFSRA+YGLDVMLDSSFQPKLLEVTYCP
Sbjct: 795 HNVKWLDIHSRVTSMIRSVFESAAVVHPEMHSPFSRAMYGLDVMLDSSFQPKLLEVTYCP 854

Query: 885 DCTRACKYDVENVY-GGVIKGKEFYNDVFGCLFLNETTHVTPL 921
           DCTRACKYDVENV+ GGVI+G+EFYN +FGCLFLNETTHVTPL
Sbjct: 855 DCTRACKYDVENVFGGGVIEGREFYNYIFGCLFLNETTHVTPL 871

BLAST of Lsi06G003350 vs. ExPASy TrEMBL
Match: A0A0A0LWN5 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G596500 PE=4 SV=1)

HSP 1 Score: 1522.3 bits (3940), Expect = 0.0e+00
Identity = 758/887 (85.46%), Postives = 788/887 (88.84%), Query Frame = 0

Query: 45  RISSKFMVCSSLPLDF-PSLCTKLTSETFDGGAHFQVEPFEDGRCRRLVLSSDFMAKESH 104
           ++    +  S LP      L  KLTSETFDGG+HFQVE FEDGRCRRLVLSSD MAKESH
Sbjct: 15  KVHGLLLTASGLPQSLHRQLFQKLTSETFDGGSHFQVEQFEDGRCRRLVLSSDCMAKESH 74

Query: 105 VFLVDHAWTFRLSDAYTQLLEVPGLAERMASLMCVDVDMNLDEEEEDQSKSND------D 164
           VF+VDHAWTFRLSDAY QLLEVPGLAERMASLMCVD+D+NL EE+ED SKSND      D
Sbjct: 75  VFVVDHAWTFRLSDAYKQLLEVPGLAERMASLMCVDIDLNLAEEDEDHSKSNDDGDGDGD 134

Query: 165 EAKQSVWELLESEIRGAKEKGNDSVRWLELEDLQIEDDTLLSLDLPTKFPDLLALSLTGN 224
           +AKQSVWEL+ESEIRGAKEKGNDSVRWLELEDLQI+DD LLSLDLPTKFPDLLALSLTGN
Sbjct: 135 DAKQSVWELIESEIRGAKEKGNDSVRWLELEDLQIDDDALLSLDLPTKFPDLLALSLTGN 194

Query: 225 KLRNVDVIAQEVAKFKHLRALWLNDNPVAENWYFSELFLFSDENLQQKVLEGSPNLEIYN 284
           KL++VDV+A+EVAKFKHLRALWLNDNPVAEN          D NLQQKVLE SPNLEIYN
Sbjct: 195 KLKDVDVVAREVAKFKHLRALWLNDNPVAEN---------CDANLQQKVLEASPNLEIYN 254

Query: 285 SRFTLNFSKWALGFCGDMYGKDNPGSIYPADHTLQCLTSLDLSSRYIHNLINKAFSPVEL 344
           SRFTLNFSKWALGFCGDMYGKDNPGSIYP+DHTLQCLTSLDLSSR IHNLINKAFSPVEL
Sbjct: 255 SRFTLNFSKWALGFCGDMYGKDNPGSIYPSDHTLQCLTSLDLSSRCIHNLINKAFSPVEL 314

Query: 345 PSLLYLNLKGNPLEQNSVGDLLKVLKEFPCLSSLEVDIPGPLGEKASDIIESLPNLNALN 404
           PSL YLNL+GNPLEQNSVGDLLK+LKEFPCLSSLEVDIPGPLGEKASDIIESLPNL+ LN
Sbjct: 315 PSLSYLNLRGNPLEQNSVGDLLKILKEFPCLSSLEVDIPGPLGEKASDIIESLPNLSNLN 374

Query: 405 GIDVAKILNSGKHVIDSMLLPRLPEWAPEEPLPDRVINAMWQYLMTYRLADEEKIDETSV 464
           GIDVAKILNSGKHVIDSMLLPRLPEWAPEE LPDRVINAMWQYLMTYRLADEEKIDETSV
Sbjct: 375 GIDVAKILNSGKHVIDSMLLPRLPEWAPEETLPDRVINAMWQYLMTYRLADEEKIDETSV 434

Query: 465 WYAVYRLGLSYFSIFSRMYVMDELGSALRHSDEPNFRVAPFLFMPEGELTSAISFTILWP 524
           W                 YVMDELGSALRHSDEPNFRVAPFLFMPEG L SAISFTILWP
Sbjct: 435 W-----------------YVMDELGSALRHSDEPNFRVAPFLFMPEGNLMSAISFTILWP 494

Query: 525 IHNVQKGDECTRDYLFGFGEDKQRSARLTAWFHTPQNYFVHEYEKHIRNLQSKALASSIS 584
           IHNVQKGDECTRDYLFG GEDKQRSARLTAWFHTPQNYFVHEYEKHI+NLQSK L S IS
Sbjct: 495 IHNVQKGDECTRDYLFGIGEDKQRSARLTAWFHTPQNYFVHEYEKHIKNLQSKVLTSPIS 554

Query: 585 QTTSNPEGLHQSKGGTFHVYTDNPQVEEFLNRPEFIMTSDPKEADIIWTSMQIDEDTRKA 644
           QTTS  E L QSKGGT  VYTDNPQVEEFLNRPEF +TSDPKEADIIWTSMQIDEDTRKA
Sbjct: 555 QTTSKTEELCQSKGGTLRVYTDNPQVEEFLNRPEFTITSDPKEADIIWTSMQIDEDTRKA 614

Query: 645 TGITDQQYVNQFPFEACLVMKHHLAETVEKAHGCPEWLQPTYNLETHLSQLIGDYLVRKR 704
           TGITD+QYVNQFPFEACLVMKHHLAET+EKAHGCPEWLQPTYNLETHLSQLIGDY VRKR
Sbjct: 615 TGITDKQYVNQFPFEACLVMKHHLAETIEKAHGCPEWLQPTYNLETHLSQLIGDYFVRKR 674

Query: 705 DGLNNLWILKPWNMARTIDTTVTDNLSAIIRLMETGPKICQKYIEHPALFNGKKFDLRYI 764
           D LNNLWILKPWNMARTIDTTVTDNLSAIIRLMETGPKICQKYIEHPALFNGKKFDLRYI
Sbjct: 675 DRLNNLWILKPWNMARTIDTTVTDNLSAIIRLMETGPKICQKYIEHPALFNGKKFDLRYI 734

Query: 765 VLVRSMKPLEIFLADSFWVRLANNPYSLETQSLFEYETHFTVMFYLAVSVACNSSWCARE 824
           VLVRSMKPLEIFLADSFWVRLANNPYSLE QSLFEYETHFTVM Y       N +   RE
Sbjct: 735 VLVRSMKPLEIFLADSFWVRLANNPYSLEKQSLFEYETHFTVMNYRGRLNHKNIADFVRE 794

Query: 825 IDYN---AWLDIHSRVRSMIRSVFESAMVVHPEMHSPFSRAIYGLDVMLDSSFQPKLLEV 884
            +      WLDIHSRVRSMIRSVFESA VVHPEMHSPFSRA+YGLDVMLDSSFQPKLLEV
Sbjct: 795 FEQEHNVKWLDIHSRVRSMIRSVFESAAVVHPEMHSPFSRAMYGLDVMLDSSFQPKLLEV 854

Query: 885 TYCPDCTRACKYDVENVYGG-VIKGKEFYNDVFGCLFLNETTHVTPL 921
           TYCPDCTRACKYDVENV+GG +IKG+ FYN +FGCLFLNETTHVTPL
Sbjct: 855 TYCPDCTRACKYDVENVFGGEIIKGEGFYNYIFGCLFLNETTHVTPL 875

BLAST of Lsi06G003350 vs. ExPASy TrEMBL
Match: A0A6J1F3Y4 (tubulin--tyrosine ligase-like protein 12 OS=Cucurbita moschata OX=3662 GN=LOC111439992 PE=4 SV=1)

HSP 1 Score: 1491.5 bits (3860), Expect = 0.0e+00
Identity = 742/882 (84.13%), Postives = 779/882 (88.32%), Query Frame = 0

Query: 44  LRISSKFMVCSSLPLDF-PSLCTKLTSETFDGGAHFQVEPFEDGRCRRLVLSSDFMAKES 103
           + +    +  S LP      L  KLTSETFDGGAHFQVEPFEDGRCRRLVLSSDFM+KES
Sbjct: 14  VEVHGLLLAASGLPQSLHRQLFQKLTSETFDGGAHFQVEPFEDGRCRRLVLSSDFMSKES 73

Query: 104 HVFLVDHAWTFRLSDAYTQLLEVPGLAERMASLMCVDVDMNLDEEEEDQSKSNDDEAKQS 163
           HVFLVDHAWTFRLSDAY QLLEVPGLAERMASLMCVDVDMNLDE+ ++     ++  +QS
Sbjct: 74  HVFLVDHAWTFRLSDAYNQLLEVPGLAERMASLMCVDVDMNLDEDAKE-----EEVDQQS 133

Query: 164 VWELLESEIRGAKEKGNDSVRWLELEDLQIEDDTLLSLDLPTKFPDLLALSLTGNKLRNV 223
           VWELLESEI GAKEKGN SVRWLELEDLQI+DDTLLSLDLPTKFPDLLALSLTGNKL NV
Sbjct: 134 VWELLESEIHGAKEKGNGSVRWLELEDLQIDDDTLLSLDLPTKFPDLLALSLTGNKLSNV 193

Query: 224 DVIAQEVAKFKHLRALWLNDNPVAENWYFSELFLFSDENLQQKVLEGSPNLEIYNSRFTL 283
           DV+AQEVAKFKHLRALWLN+NP  EN          DE LQQKVL+GSPNLEIYNSRFT 
Sbjct: 194 DVVAQEVAKFKHLRALWLNNNPAVEN---------CDEILQQKVLDGSPNLEIYNSRFTH 253

Query: 284 NFSKWALGFCGDMYGKDNPGSIYPADHTLQCLTSLDLSSRYIHNLINKAFSPVELPSLLY 343
           NFS+WALGFCGD+Y KDNPGSIYPADH+LQ +TSLDLS R IHNL NKAFSPVELPSL Y
Sbjct: 254 NFSEWALGFCGDIYEKDNPGSIYPADHSLQDVTSLDLSHRGIHNLNNKAFSPVELPSLSY 313

Query: 344 LNLKGNPLEQNSVGDLLKVLKEFPCLSSLEVDIPGPLGEKASDIIESLPNLNALNGIDVA 403
           LNL+GNPLEQNSVGDLL++LKEFPCLSSLEVDIPGPLGEKA DIIESLPNL+ LN IDV 
Sbjct: 314 LNLRGNPLEQNSVGDLLEILKEFPCLSSLEVDIPGPLGEKAVDIIESLPNLHTLNSIDVK 373

Query: 404 KILNSGKHVIDSMLLPRLPEWAPEEPLPDRVINAMWQYLMTYRLADEEKIDETSVWYAVY 463
           KILNSGKHVIDSMLLPRLPEW PEEPLPDRVINAMWQYLMTYRLADEEKIDETSVW    
Sbjct: 374 KILNSGKHVIDSMLLPRLPEWTPEEPLPDRVINAMWQYLMTYRLADEEKIDETSVW---- 433

Query: 464 RLGLSYFSIFSRMYVMDELGSALRHSDEPNFRVAPFLFMPEGELTSAISFTILWPIHNVQ 523
                        YVMDELGSALRHSDEPNFRVAPFLFMP+G+L SAISFTILWPI +VQ
Sbjct: 434 -------------YVMDELGSALRHSDEPNFRVAPFLFMPDGKLASAISFTILWPILDVQ 493

Query: 524 KGDECTRDYLFGFGEDKQRSARLTAWFHTPQNYFVHEYEKHIRNLQSKALASSISQTTSN 583
           KGDECTRDYLFG GEDKQRSARLTAWFHTPQNYFVHEYEK+IRNLQSKALAS I QTTSN
Sbjct: 494 KGDECTRDYLFGIGEDKQRSARLTAWFHTPQNYFVHEYEKYIRNLQSKALASPIPQTTSN 553

Query: 584 PEGLHQSKGGTFHVYTDNPQVEEFLNRPEFIMTSDPKEADIIWTSMQIDEDTRKATGITD 643
            EG HQ KGGT  VYTDNPQVEE+LNRPEFI+TSDPKEADIIWTSMQIDE+T+KATGITD
Sbjct: 554 TEGRHQIKGGTLRVYTDNPQVEEYLNRPEFIITSDPKEADIIWTSMQIDEETKKATGITD 613

Query: 644 QQYVNQFPFEACLVMKHHLAETVEKAHGCPEWLQPTYNLETHLSQLIGDYLVRKRDGLNN 703
           QQYVNQFPFEACLVMKHHLAETVEKAHGCPEWLQPTYNLETHLSQLIGDYLVRKRDGLNN
Sbjct: 614 QQYVNQFPFEACLVMKHHLAETVEKAHGCPEWLQPTYNLETHLSQLIGDYLVRKRDGLNN 673

Query: 704 LWILKPWNMARTIDTTVTDNLSAIIRLMETGPKICQKYIEHPALFNGKKFDLRYIVLVRS 763
           LWILKPWNMARTIDTTVTDNLSAIIRLMETGPKICQKYIEHPALFNGKKFDLRYIVLVRS
Sbjct: 674 LWILKPWNMARTIDTTVTDNLSAIIRLMETGPKICQKYIEHPALFNGKKFDLRYIVLVRS 733

Query: 764 MKPLEIFLADSFWVRLANNPYSLETQSLFEYETHFTVMFYLAVSVACNSSWCAREIDYN- 823
           MKPLEIFLADSFWVRLANNPYSLE QSLFEYETHFTVM Y       N +   +E +   
Sbjct: 734 MKPLEIFLADSFWVRLANNPYSLEKQSLFEYETHFTVMNYRGKLNHKNIADFVKEFEQEH 793

Query: 824 --AWLDIHSRVRSMIRSVFESAMVVHPEMHSPFSRAIYGLDVMLDSSFQPKLLEVTYCPD 883
              WLDIHSRVRSMIRSVFESA VVHPEMHSPFSRA+YGLDVMLDSSFQPKLLEVTYCPD
Sbjct: 794 NVKWLDIHSRVRSMIRSVFESAAVVHPEMHSPFSRAMYGLDVMLDSSFQPKLLEVTYCPD 853

Query: 884 CTRACKYDVENVYG-GVIKGKEFYNDVFGCLFLNETTHVTPL 921
           CTRACKYDVENV+G G+IKG+EFYNDVFGCLFLNETTH TPL
Sbjct: 854 CTRACKYDVENVFGDGIIKGREFYNDVFGCLFLNETTHATPL 864

BLAST of Lsi06G003350 vs. NCBI nr
Match: XP_038879092.1 (tubulin--tyrosine ligase-like protein 12 [Benincasa hispida])

HSP 1 Score: 1541.2 bits (3989), Expect = 0.0e+00
Identity = 767/883 (86.86%), Postives = 789/883 (89.35%), Query Frame = 0

Query: 44  LRISSKFMVCSSLPLDF-PSLCTKLTSETFDGGAHFQVEPFEDGRCRRLVLSSDFMAKES 103
           + +    +  S LP      L  KLTSETFDGGAHFQVEPFEDGRCRRLVLSSDFMAKES
Sbjct: 14  VEVHGLLLTASGLPQSLHRQLFLKLTSETFDGGAHFQVEPFEDGRCRRLVLSSDFMAKES 73

Query: 104 HVFLVDHAWTFRLSDAYTQLLEVPGLAERMASLMCVDVDMNLDEEEEDQSKSNDDEAKQS 163
           HVFLVDHAWTFRLSDAY QLLEVPGLAERMASLMCVD+DMNLDEE ED SKSNDDEAKQ 
Sbjct: 74  HVFLVDHAWTFRLSDAYKQLLEVPGLAERMASLMCVDIDMNLDEEGEDYSKSNDDEAKQG 133

Query: 164 VWELLESEIRGAKEKGNDSVRWLELEDLQIEDDTLLSLDLPTKFPDLLALSLTGNKLRNV 223
           VWELLESE+RGAKEKGNDSVRWLELEDLQI DDTLLSLDLPTKFPDLLALSLTGNKLRNV
Sbjct: 134 VWELLESEVRGAKEKGNDSVRWLELEDLQINDDTLLSLDLPTKFPDLLALSLTGNKLRNV 193

Query: 224 DVIAQEVAKFKHLRALWLNDNPVAENWYFSELFLFSDENLQQKVLEGSPNLEIYNSRFTL 283
           DV+AQEVAKFKHLRALWLNDNPVAEN          DENL QKVLEGS NLEIYNSRFTL
Sbjct: 194 DVVAQEVAKFKHLRALWLNDNPVAEN---------CDENLLQKVLEGSSNLEIYNSRFTL 253

Query: 284 NFSKWALGFCGDMYGKDNPGSIYPADHTLQCLTSLDLSSRYIHNLINKAFSPVELPSLLY 343
           N+SKWALGFCGD+Y KDNPGSIYPADHTLQCLT LDLSSR IHNLINKAFSPVELPSL Y
Sbjct: 254 NYSKWALGFCGDVYEKDNPGSIYPADHTLQCLTRLDLSSRCIHNLINKAFSPVELPSLSY 313

Query: 344 LNLKGNPLEQNSVGDLLKVLKEFPCLSSLEVDIPGPLGEKASDIIESLPNLNALNGIDVA 403
           LNL+GNPLEQNSVGDLLKVLKEFPCLSSLEVDIPGPLGEKASDII+SLPNL  LNGIDVA
Sbjct: 314 LNLRGNPLEQNSVGDLLKVLKEFPCLSSLEVDIPGPLGEKASDIIKSLPNLKTLNGIDVA 373

Query: 404 KILNSGKHVIDSMLLPRLPEWAPEEPLPDRVINAMWQYLMTYRLADEEKIDETSVWYAVY 463
           KILNSGKHVIDSMLLPRLPEWAPEEPLPDRVINAMWQYLMTYRLADEEKIDETSVW    
Sbjct: 374 KILNSGKHVIDSMLLPRLPEWAPEEPLPDRVINAMWQYLMTYRLADEEKIDETSVW---- 433

Query: 464 RLGLSYFSIFSRMYVMDELGSALRHSDEPNFRVAPFLFMPEGELTSAISFTILWPIHNVQ 523
                        YVMDELGS LRHSDEPNF VAPFLFMPEG LTSAISFT+LWPIHNVQ
Sbjct: 434 -------------YVMDELGSVLRHSDEPNFSVAPFLFMPEGNLTSAISFTLLWPIHNVQ 493

Query: 524 KGDECTRDYLFGFGEDKQRSARLTAWFHTPQNYFVHEYEKHIRNLQSKALASSISQTTSN 583
           KGDECTRDYLFG GEDKQRSARLTAWFHTPQNYFV+EYEKHIRNL+ KALASSISQTTSN
Sbjct: 494 KGDECTRDYLFGIGEDKQRSARLTAWFHTPQNYFVNEYEKHIRNLRWKALASSISQTTSN 553

Query: 584 PEGLHQSKGGTFHVYTDNPQVEEFLNRPEFIMTSDPKEADIIWTSMQIDEDTRKATGITD 643
            EGLHQSKGG  HVYTDNPQVEEFLNRPEFIMTSDPKEADIIWTSMQIDE+TRKATGITD
Sbjct: 554 TEGLHQSKGGILHVYTDNPQVEEFLNRPEFIMTSDPKEADIIWTSMQIDEETRKATGITD 613

Query: 644 QQYVNQFPFEACLVMKHHLAETVEKAHGCPEWLQPTYNLETHLSQLIGDYLVRKRDGLNN 703
           QQYVNQFPFEACLVMKHHLAETVEKAHGC EWLQPTYNLETHLSQLIGDY VRKRDGLNN
Sbjct: 614 QQYVNQFPFEACLVMKHHLAETVEKAHGCTEWLQPTYNLETHLSQLIGDYFVRKRDGLNN 673

Query: 704 LWILKPWNMARTIDTTVTDNLSAIIRLMETGPKICQKYIEHPALFNGKKFDLRYIVLVRS 763
           LWILKPWNMARTIDTTVTDNLS+IIRLMETGPKICQKYIEHPALFNGKKFDLRYIVLVRS
Sbjct: 674 LWILKPWNMARTIDTTVTDNLSSIIRLMETGPKICQKYIEHPALFNGKKFDLRYIVLVRS 733

Query: 764 MKPLEIFLADSFWVRLANNPYSLETQSLFEYETHFTVMFYLAVSVACNSSWCAREIDYN- 823
           MKPLEIFLADSFWVRLANNPYSLE +SLFEYETHFTVM Y       N +   +E +   
Sbjct: 734 MKPLEIFLADSFWVRLANNPYSLEKKSLFEYETHFTVMNYRGRLNHKNIADFVKEFEQEH 793

Query: 824 --AWLDIHSRVRSMIRSVFESAMVVHPEMHSPFSRAIYGLDVMLDSSFQPKLLEVTYCPD 883
              WLDIHS VRSMIRSVFESA VVHPEMHSPFSRA+YGLDVMLDSSFQPKLLEVTYCPD
Sbjct: 794 NVKWLDIHSTVRSMIRSVFESAAVVHPEMHSPFSRAMYGLDVMLDSSFQPKLLEVTYCPD 853

Query: 884 CTRACKYDVENVYGG--VIKGKEFYNDVFGCLFLNETTHVTPL 921
           C RACKYDVENV G   V+KG+EFYN VFGCLFLNETTHVTPL
Sbjct: 854 CNRACKYDVENVLGDGEVVKGQEFYNYVFGCLFLNETTHVTPL 870

BLAST of Lsi06G003350 vs. NCBI nr
Match: TYK10026.1 (tubulin--tyrosine ligase-like protein 12 [Cucumis melo var. makuwa])

HSP 1 Score: 1530.4 bits (3961), Expect = 0.0e+00
Identity = 758/883 (85.84%), Postives = 791/883 (89.58%), Query Frame = 0

Query: 45  RISSKFMVCSSLPLDF-PSLCTKLTSETFDGGAHFQVEPFEDGRCRRLVLSSDFMAKESH 104
           ++    +  S LP      L  KLTSETFDGG+HFQVEPFEDGRCRRLVLSSDFMAKESH
Sbjct: 15  KVHGLLLTASGLPQSLHRQLFQKLTSETFDGGSHFQVEPFEDGRCRRLVLSSDFMAKESH 74

Query: 105 VFLVDHAWTFRLSDAYTQLLEVPGLAERMASLMCVDVDMNLDEEEEDQSKSN--DDEAKQ 164
           VFLVDHAWTFRLSDAY QLLEVPGLAERMASLMCVD+D+NLDEE+ED SKSN  DD+AKQ
Sbjct: 75  VFLVDHAWTFRLSDAYKQLLEVPGLAERMASLMCVDIDLNLDEEDEDHSKSNVDDDDAKQ 134

Query: 165 SVWELLESEIRGAKEKGNDSVRWLELEDLQIEDDTLLSLDLPTKFPDLLALSLTGNKLRN 224
           SVWEL+ESEIRGA EKGNDSVRWLELEDLQI+DDTLLSLDLPTKFPDLLALSLTGNKL++
Sbjct: 135 SVWELIESEIRGAIEKGNDSVRWLELEDLQIDDDTLLSLDLPTKFPDLLALSLTGNKLKD 194

Query: 225 VDVIAQEVAKFKHLRALWLNDNPVAENWYFSELFLFSDENLQQKVLEGSPNLEIYNSRFT 284
           VD +A+EV KFKHLRALWLNDNPVAEN          D +LQQ+VLEGSPNLEIYNSRFT
Sbjct: 195 VDTVAREVVKFKHLRALWLNDNPVAEN---------CDASLQQRVLEGSPNLEIYNSRFT 254

Query: 285 LNFSKWALGFCGDMYGKDNPGSIYPADHTLQCLTSLDLSSRYIHNLINKAFSPVELPSLL 344
           LNFSKWALGFCGDMYGKDNPGSIYP+DHTLQCLTSLDLSSR IHNLINKAFSPVELPSL 
Sbjct: 255 LNFSKWALGFCGDMYGKDNPGSIYPSDHTLQCLTSLDLSSRCIHNLINKAFSPVELPSLS 314

Query: 345 YLNLKGNPLEQNSVGDLLKVLKEFPCLSSLEVDIPGPLGEKASDIIESLPNLNALNGIDV 404
           YLNL+GNPLEQNSVGDLLK+LKEFPCLSSLEVDIPGPLGEKASDIIESLPNL+ LNGIDV
Sbjct: 315 YLNLRGNPLEQNSVGDLLKILKEFPCLSSLEVDIPGPLGEKASDIIESLPNLSNLNGIDV 374

Query: 405 AKILNSGKHVIDSMLLPRLPEWAPEEPLPDRVINAMWQYLMTYRLADEEKIDETSVWYAV 464
            KIL+SGKH+IDSMLLPRLPEWAPEE LPDRVINAMWQYLMTYRLADEEKIDETSVW   
Sbjct: 375 EKILSSGKHMIDSMLLPRLPEWAPEETLPDRVINAMWQYLMTYRLADEEKIDETSVW--- 434

Query: 465 YRLGLSYFSIFSRMYVMDELGSALRHSDEPNFRVAPFLFMPEGELTSAISFTILWPIHNV 524
                         YVMDELGSALRHSDEPNFRVAPFLFMPEG LTSAISFTILWPIHNV
Sbjct: 435 --------------YVMDELGSALRHSDEPNFRVAPFLFMPEGNLTSAISFTILWPIHNV 494

Query: 525 QKGDECTRDYLFGFGEDKQRSARLTAWFHTPQNYFVHEYEKHIRNLQSKALASSISQTTS 584
           QKGDECTRDYLFG GEDKQRSARLTAWFHTPQNYFVHEYEKHI+NLQSK L S ISQTTS
Sbjct: 495 QKGDECTRDYLFGIGEDKQRSARLTAWFHTPQNYFVHEYEKHIKNLQSKVLTSPISQTTS 554

Query: 585 NPEGLHQSKGGTFHVYTDNPQVEEFLNRPEFIMTSDPKEADIIWTSMQIDEDTRKATGIT 644
             E L QSKGGT  VYTDNPQVEEFLNRPEF +TSDPKEADIIWTSMQIDEDTRKATGIT
Sbjct: 555 KTEELRQSKGGTLRVYTDNPQVEEFLNRPEFTITSDPKEADIIWTSMQIDEDTRKATGIT 614

Query: 645 DQQYVNQFPFEACLVMKHHLAETVEKAHGCPEWLQPTYNLETHLSQLIGDYLVRKRDGLN 704
           D+QYVNQFPFEACLVMKHHLAET+EKAHGCPEWLQPTYNLETHLSQLIGDY VRKRDGLN
Sbjct: 615 DKQYVNQFPFEACLVMKHHLAETIEKAHGCPEWLQPTYNLETHLSQLIGDYFVRKRDGLN 674

Query: 705 NLWILKPWNMARTIDTTVTDNLSAIIRLMETGPKICQKYIEHPALFNGKKFDLRYIVLVR 764
           NLWILKPWNMARTIDTTVTDNLSAIIRLMETGPKICQKYIEHPALFNGKKFDLRYIVLVR
Sbjct: 675 NLWILKPWNMARTIDTTVTDNLSAIIRLMETGPKICQKYIEHPALFNGKKFDLRYIVLVR 734

Query: 765 SMKPLEIFLADSFWVRLANNPYSLETQSLFEYETHFTVMFYLAVSVACNSSWCAREIDYN 824
           SMKPLEIFLADSFWVRLANNPYSLE QSLFEYETHFTVM Y       N +   RE +  
Sbjct: 735 SMKPLEIFLADSFWVRLANNPYSLEKQSLFEYETHFTVMNYRGRLNHKNIADFVREFEQE 794

Query: 825 ---AWLDIHSRVRSMIRSVFESAMVVHPEMHSPFSRAIYGLDVMLDSSFQPKLLEVTYCP 884
               WLDIHSRV SMIRSVFESA VVHPEMHSPFSRA+YGLDVMLDSSFQPKLLEVTYCP
Sbjct: 795 HNVKWLDIHSRVTSMIRSVFESAAVVHPEMHSPFSRAMYGLDVMLDSSFQPKLLEVTYCP 854

Query: 885 DCTRACKYDVENVY-GGVIKGKEFYNDVFGCLFLNETTHVTPL 921
           DCTRACKYDVENV+ GGVI+G+EFYN +FGCLFLNETTHVTPL
Sbjct: 855 DCTRACKYDVENVFGGGVIEGREFYNYIFGCLFLNETTHVTPL 871

BLAST of Lsi06G003350 vs. NCBI nr
Match: XP_008450923.1 (PREDICTED: tubulin--tyrosine ligase-like protein 12 [Cucumis melo] >KAA0055775.1 tubulin--tyrosine ligase-like protein 12 [Cucumis melo var. makuwa])

HSP 1 Score: 1528.5 bits (3956), Expect = 0.0e+00
Identity = 757/883 (85.73%), Postives = 791/883 (89.58%), Query Frame = 0

Query: 45  RISSKFMVCSSLPLDF-PSLCTKLTSETFDGGAHFQVEPFEDGRCRRLVLSSDFMAKESH 104
           ++    +  S LP      L  KLTSETFDGG+HFQVEPFEDGRCRRLVLSSDFMAKESH
Sbjct: 15  KVHGLLLTASGLPQSLHRQLFQKLTSETFDGGSHFQVEPFEDGRCRRLVLSSDFMAKESH 74

Query: 105 VFLVDHAWTFRLSDAYTQLLEVPGLAERMASLMCVDVDMNLDEEEEDQSKSN--DDEAKQ 164
           VFLVDHAWTFRLSDAY QLLEVPGLAERMASLMCVD+D+NLDEE+ED SKSN  DD+AKQ
Sbjct: 75  VFLVDHAWTFRLSDAYKQLLEVPGLAERMASLMCVDIDLNLDEEDEDHSKSNVDDDDAKQ 134

Query: 165 SVWELLESEIRGAKEKGNDSVRWLELEDLQIEDDTLLSLDLPTKFPDLLALSLTGNKLRN 224
           SVWEL+ESEIRGA EKGNDSVRWLELE+LQI+DDTLLSLDLPTKFPDLLALSLTGNKL++
Sbjct: 135 SVWELIESEIRGAIEKGNDSVRWLELENLQIDDDTLLSLDLPTKFPDLLALSLTGNKLKD 194

Query: 225 VDVIAQEVAKFKHLRALWLNDNPVAENWYFSELFLFSDENLQQKVLEGSPNLEIYNSRFT 284
           VD +A+EV KFKHLRALWLNDNPVAEN          D +LQQ+VLEGSPNLEIYNSRFT
Sbjct: 195 VDTVAREVVKFKHLRALWLNDNPVAEN---------CDASLQQRVLEGSPNLEIYNSRFT 254

Query: 285 LNFSKWALGFCGDMYGKDNPGSIYPADHTLQCLTSLDLSSRYIHNLINKAFSPVELPSLL 344
           LNFSKWALGFCGDMYGKDNPGSIYP+DHTLQCLTSLDLSSR IHNLINKAFSPVELPSL 
Sbjct: 255 LNFSKWALGFCGDMYGKDNPGSIYPSDHTLQCLTSLDLSSRCIHNLINKAFSPVELPSLS 314

Query: 345 YLNLKGNPLEQNSVGDLLKVLKEFPCLSSLEVDIPGPLGEKASDIIESLPNLNALNGIDV 404
           YLNL+GNPLEQNSVGDLLK+LKEFPCLSSLEVDIPGPLGEKASDIIESLPNL+ LNGIDV
Sbjct: 315 YLNLRGNPLEQNSVGDLLKILKEFPCLSSLEVDIPGPLGEKASDIIESLPNLSNLNGIDV 374

Query: 405 AKILNSGKHVIDSMLLPRLPEWAPEEPLPDRVINAMWQYLMTYRLADEEKIDETSVWYAV 464
            KIL+SGKH+IDSMLLPRLPEWAPEE LPDRVINAMWQYLMTYRLADEEKIDETSVW   
Sbjct: 375 EKILSSGKHMIDSMLLPRLPEWAPEETLPDRVINAMWQYLMTYRLADEEKIDETSVW--- 434

Query: 465 YRLGLSYFSIFSRMYVMDELGSALRHSDEPNFRVAPFLFMPEGELTSAISFTILWPIHNV 524
                         YVMDELGSALRHSDEPNFRVAPFLFMPEG LTSAISFTILWPIHNV
Sbjct: 435 --------------YVMDELGSALRHSDEPNFRVAPFLFMPEGNLTSAISFTILWPIHNV 494

Query: 525 QKGDECTRDYLFGFGEDKQRSARLTAWFHTPQNYFVHEYEKHIRNLQSKALASSISQTTS 584
           QKGDECTRDYLFG GEDKQRSARLTAWFHTPQNYFVHEYEKHI+NLQSK L S ISQTTS
Sbjct: 495 QKGDECTRDYLFGIGEDKQRSARLTAWFHTPQNYFVHEYEKHIKNLQSKVLTSPISQTTS 554

Query: 585 NPEGLHQSKGGTFHVYTDNPQVEEFLNRPEFIMTSDPKEADIIWTSMQIDEDTRKATGIT 644
             E L QSKGGT  VYTDNPQVEEFLNRPEF +TSDPKEADIIWTSMQIDEDTRKATGIT
Sbjct: 555 KTEELRQSKGGTLRVYTDNPQVEEFLNRPEFTITSDPKEADIIWTSMQIDEDTRKATGIT 614

Query: 645 DQQYVNQFPFEACLVMKHHLAETVEKAHGCPEWLQPTYNLETHLSQLIGDYLVRKRDGLN 704
           D+QYVNQFPFEACLVMKHHLAET+EKAHGCPEWLQPTYNLETHLSQLIGDY VRKRDGLN
Sbjct: 615 DKQYVNQFPFEACLVMKHHLAETIEKAHGCPEWLQPTYNLETHLSQLIGDYFVRKRDGLN 674

Query: 705 NLWILKPWNMARTIDTTVTDNLSAIIRLMETGPKICQKYIEHPALFNGKKFDLRYIVLVR 764
           NLWILKPWNMARTIDTTVTDNLSAIIRLMETGPKICQKYIEHPALFNGKKFDLRYIVLVR
Sbjct: 675 NLWILKPWNMARTIDTTVTDNLSAIIRLMETGPKICQKYIEHPALFNGKKFDLRYIVLVR 734

Query: 765 SMKPLEIFLADSFWVRLANNPYSLETQSLFEYETHFTVMFYLAVSVACNSSWCAREIDYN 824
           SMKPLEIFLADSFWVRLANNPYSLE QSLFEYETHFTVM Y       N +   RE +  
Sbjct: 735 SMKPLEIFLADSFWVRLANNPYSLEKQSLFEYETHFTVMNYRGRLNHKNIADFVREFEQE 794

Query: 825 ---AWLDIHSRVRSMIRSVFESAMVVHPEMHSPFSRAIYGLDVMLDSSFQPKLLEVTYCP 884
               WLDIHSRV SMIRSVFESA VVHPEMHSPFSRA+YGLDVMLDSSFQPKLLEVTYCP
Sbjct: 795 HNVKWLDIHSRVTSMIRSVFESAAVVHPEMHSPFSRAMYGLDVMLDSSFQPKLLEVTYCP 854

Query: 885 DCTRACKYDVENVY-GGVIKGKEFYNDVFGCLFLNETTHVTPL 921
           DCTRACKYDVENV+ GGVI+G+EFYN +FGCLFLNETTHVTPL
Sbjct: 855 DCTRACKYDVENVFGGGVIEGREFYNYIFGCLFLNETTHVTPL 871

BLAST of Lsi06G003350 vs. NCBI nr
Match: XP_004144031.1 (tubulin--tyrosine ligase-like protein 12 [Cucumis sativus] >KGN66300.1 hypothetical protein Csa_007663 [Cucumis sativus])

HSP 1 Score: 1522.3 bits (3940), Expect = 0.0e+00
Identity = 758/887 (85.46%), Postives = 788/887 (88.84%), Query Frame = 0

Query: 45  RISSKFMVCSSLPLDF-PSLCTKLTSETFDGGAHFQVEPFEDGRCRRLVLSSDFMAKESH 104
           ++    +  S LP      L  KLTSETFDGG+HFQVE FEDGRCRRLVLSSD MAKESH
Sbjct: 15  KVHGLLLTASGLPQSLHRQLFQKLTSETFDGGSHFQVEQFEDGRCRRLVLSSDCMAKESH 74

Query: 105 VFLVDHAWTFRLSDAYTQLLEVPGLAERMASLMCVDVDMNLDEEEEDQSKSND------D 164
           VF+VDHAWTFRLSDAY QLLEVPGLAERMASLMCVD+D+NL EE+ED SKSND      D
Sbjct: 75  VFVVDHAWTFRLSDAYKQLLEVPGLAERMASLMCVDIDLNLAEEDEDHSKSNDDGDGDGD 134

Query: 165 EAKQSVWELLESEIRGAKEKGNDSVRWLELEDLQIEDDTLLSLDLPTKFPDLLALSLTGN 224
           +AKQSVWEL+ESEIRGAKEKGNDSVRWLELEDLQI+DD LLSLDLPTKFPDLLALSLTGN
Sbjct: 135 DAKQSVWELIESEIRGAKEKGNDSVRWLELEDLQIDDDALLSLDLPTKFPDLLALSLTGN 194

Query: 225 KLRNVDVIAQEVAKFKHLRALWLNDNPVAENWYFSELFLFSDENLQQKVLEGSPNLEIYN 284
           KL++VDV+A+EVAKFKHLRALWLNDNPVAEN          D NLQQKVLE SPNLEIYN
Sbjct: 195 KLKDVDVVAREVAKFKHLRALWLNDNPVAEN---------CDANLQQKVLEASPNLEIYN 254

Query: 285 SRFTLNFSKWALGFCGDMYGKDNPGSIYPADHTLQCLTSLDLSSRYIHNLINKAFSPVEL 344
           SRFTLNFSKWALGFCGDMYGKDNPGSIYP+DHTLQCLTSLDLSSR IHNLINKAFSPVEL
Sbjct: 255 SRFTLNFSKWALGFCGDMYGKDNPGSIYPSDHTLQCLTSLDLSSRCIHNLINKAFSPVEL 314

Query: 345 PSLLYLNLKGNPLEQNSVGDLLKVLKEFPCLSSLEVDIPGPLGEKASDIIESLPNLNALN 404
           PSL YLNL+GNPLEQNSVGDLLK+LKEFPCLSSLEVDIPGPLGEKASDIIESLPNL+ LN
Sbjct: 315 PSLSYLNLRGNPLEQNSVGDLLKILKEFPCLSSLEVDIPGPLGEKASDIIESLPNLSNLN 374

Query: 405 GIDVAKILNSGKHVIDSMLLPRLPEWAPEEPLPDRVINAMWQYLMTYRLADEEKIDETSV 464
           GIDVAKILNSGKHVIDSMLLPRLPEWAPEE LPDRVINAMWQYLMTYRLADEEKIDETSV
Sbjct: 375 GIDVAKILNSGKHVIDSMLLPRLPEWAPEETLPDRVINAMWQYLMTYRLADEEKIDETSV 434

Query: 465 WYAVYRLGLSYFSIFSRMYVMDELGSALRHSDEPNFRVAPFLFMPEGELTSAISFTILWP 524
           W                 YVMDELGSALRHSDEPNFRVAPFLFMPEG L SAISFTILWP
Sbjct: 435 W-----------------YVMDELGSALRHSDEPNFRVAPFLFMPEGNLMSAISFTILWP 494

Query: 525 IHNVQKGDECTRDYLFGFGEDKQRSARLTAWFHTPQNYFVHEYEKHIRNLQSKALASSIS 584
           IHNVQKGDECTRDYLFG GEDKQRSARLTAWFHTPQNYFVHEYEKHI+NLQSK L S IS
Sbjct: 495 IHNVQKGDECTRDYLFGIGEDKQRSARLTAWFHTPQNYFVHEYEKHIKNLQSKVLTSPIS 554

Query: 585 QTTSNPEGLHQSKGGTFHVYTDNPQVEEFLNRPEFIMTSDPKEADIIWTSMQIDEDTRKA 644
           QTTS  E L QSKGGT  VYTDNPQVEEFLNRPEF +TSDPKEADIIWTSMQIDEDTRKA
Sbjct: 555 QTTSKTEELCQSKGGTLRVYTDNPQVEEFLNRPEFTITSDPKEADIIWTSMQIDEDTRKA 614

Query: 645 TGITDQQYVNQFPFEACLVMKHHLAETVEKAHGCPEWLQPTYNLETHLSQLIGDYLVRKR 704
           TGITD+QYVNQFPFEACLVMKHHLAET+EKAHGCPEWLQPTYNLETHLSQLIGDY VRKR
Sbjct: 615 TGITDKQYVNQFPFEACLVMKHHLAETIEKAHGCPEWLQPTYNLETHLSQLIGDYFVRKR 674

Query: 705 DGLNNLWILKPWNMARTIDTTVTDNLSAIIRLMETGPKICQKYIEHPALFNGKKFDLRYI 764
           D LNNLWILKPWNMARTIDTTVTDNLSAIIRLMETGPKICQKYIEHPALFNGKKFDLRYI
Sbjct: 675 DRLNNLWILKPWNMARTIDTTVTDNLSAIIRLMETGPKICQKYIEHPALFNGKKFDLRYI 734

Query: 765 VLVRSMKPLEIFLADSFWVRLANNPYSLETQSLFEYETHFTVMFYLAVSVACNSSWCARE 824
           VLVRSMKPLEIFLADSFWVRLANNPYSLE QSLFEYETHFTVM Y       N +   RE
Sbjct: 735 VLVRSMKPLEIFLADSFWVRLANNPYSLEKQSLFEYETHFTVMNYRGRLNHKNIADFVRE 794

Query: 825 IDYN---AWLDIHSRVRSMIRSVFESAMVVHPEMHSPFSRAIYGLDVMLDSSFQPKLLEV 884
            +      WLDIHSRVRSMIRSVFESA VVHPEMHSPFSRA+YGLDVMLDSSFQPKLLEV
Sbjct: 795 FEQEHNVKWLDIHSRVRSMIRSVFESAAVVHPEMHSPFSRAMYGLDVMLDSSFQPKLLEV 854

Query: 885 TYCPDCTRACKYDVENVYGG-VIKGKEFYNDVFGCLFLNETTHVTPL 921
           TYCPDCTRACKYDVENV+GG +IKG+ FYN +FGCLFLNETTHVTPL
Sbjct: 855 TYCPDCTRACKYDVENVFGGEIIKGEGFYNYIFGCLFLNETTHVTPL 875

BLAST of Lsi06G003350 vs. NCBI nr
Match: KAG6588082.1 (Tubulin--tyrosine ligase-like protein 12, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1493.4 bits (3865), Expect = 0.0e+00
Identity = 743/882 (84.24%), Postives = 779/882 (88.32%), Query Frame = 0

Query: 44  LRISSKFMVCSSLPLDFP-SLCTKLTSETFDGGAHFQVEPFEDGRCRRLVLSSDFMAKES 103
           + +    +  S LP      L  KLTSETFDGGAHFQVEPFEDGRCRRLVLSSDFM+KES
Sbjct: 42  VEVHGLLLAASGLPQSLHCQLFQKLTSETFDGGAHFQVEPFEDGRCRRLVLSSDFMSKES 101

Query: 104 HVFLVDHAWTFRLSDAYTQLLEVPGLAERMASLMCVDVDMNLDEEEEDQSKSNDDEAKQS 163
           HVFLVDHAWTFRLSDAY QLLEVPGLAERMASLMCVDVDMNLDE+ ++     ++  +QS
Sbjct: 102 HVFLVDHAWTFRLSDAYNQLLEVPGLAERMASLMCVDVDMNLDEDAKE-----EEVDQQS 161

Query: 164 VWELLESEIRGAKEKGNDSVRWLELEDLQIEDDTLLSLDLPTKFPDLLALSLTGNKLRNV 223
           VWELLESEI GAKEKGNDSVRWLELEDLQI+DDTLLSLDLPTKFPDLLALSLTGNKL NV
Sbjct: 162 VWELLESEIHGAKEKGNDSVRWLELEDLQIDDDTLLSLDLPTKFPDLLALSLTGNKLSNV 221

Query: 224 DVIAQEVAKFKHLRALWLNDNPVAENWYFSELFLFSDENLQQKVLEGSPNLEIYNSRFTL 283
           DV+AQEVAKFKHLRALWLN+NP  EN          DE LQQKVL+GSPNLEIYNSRFT 
Sbjct: 222 DVVAQEVAKFKHLRALWLNNNPAVEN---------CDEILQQKVLDGSPNLEIYNSRFTH 281

Query: 284 NFSKWALGFCGDMYGKDNPGSIYPADHTLQCLTSLDLSSRYIHNLINKAFSPVELPSLLY 343
           NFSKWALGFCGD+Y KDNPGSIYPADH+LQ +TSLDLS R IHNL NKAFSPVELPSL Y
Sbjct: 282 NFSKWALGFCGDIYEKDNPGSIYPADHSLQDVTSLDLSHRCIHNLNNKAFSPVELPSLSY 341

Query: 344 LNLKGNPLEQNSVGDLLKVLKEFPCLSSLEVDIPGPLGEKASDIIESLPNLNALNGIDVA 403
           LNL+GNPLEQNSVGDLL++LKEFPCLSSLEVDIPGPLGEKA DIIESLPNL+ LN IDV 
Sbjct: 342 LNLRGNPLEQNSVGDLLEILKEFPCLSSLEVDIPGPLGEKAVDIIESLPNLHTLNSIDVK 401

Query: 404 KILNSGKHVIDSMLLPRLPEWAPEEPLPDRVINAMWQYLMTYRLADEEKIDETSVWYAVY 463
           KILNSGKHVIDSMLLPRLPEW PEEPLPDRVINAMWQYLMTYRLADEEKIDETSVW    
Sbjct: 402 KILNSGKHVIDSMLLPRLPEWTPEEPLPDRVINAMWQYLMTYRLADEEKIDETSVW---- 461

Query: 464 RLGLSYFSIFSRMYVMDELGSALRHSDEPNFRVAPFLFMPEGELTSAISFTILWPIHNVQ 523
                        YVMDELGSALRHSDEPNFRVAPFLFMP+G+L SAISFTILWPI +VQ
Sbjct: 462 -------------YVMDELGSALRHSDEPNFRVAPFLFMPDGKLASAISFTILWPILDVQ 521

Query: 524 KGDECTRDYLFGFGEDKQRSARLTAWFHTPQNYFVHEYEKHIRNLQSKALASSISQTTSN 583
           KGDECTRDYLFG GEDKQRSARLTAWFHTPQNYFVHEYEK+IRNLQSKALAS I QTTSN
Sbjct: 522 KGDECTRDYLFGIGEDKQRSARLTAWFHTPQNYFVHEYEKYIRNLQSKALASPIPQTTSN 581

Query: 584 PEGLHQSKGGTFHVYTDNPQVEEFLNRPEFIMTSDPKEADIIWTSMQIDEDTRKATGITD 643
            EG HQ KGGT  VYTDNPQVEE+LNRPEFI+TSDPKEADIIWTSMQIDE+T+KATGITD
Sbjct: 582 TEGRHQIKGGTLRVYTDNPQVEEYLNRPEFIITSDPKEADIIWTSMQIDEETKKATGITD 641

Query: 644 QQYVNQFPFEACLVMKHHLAETVEKAHGCPEWLQPTYNLETHLSQLIGDYLVRKRDGLNN 703
           QQYVNQFPFEACLVMKHHLAETVEKAHGCPEWLQPTYNLETHLSQLIGDYLVRKRDGLNN
Sbjct: 642 QQYVNQFPFEACLVMKHHLAETVEKAHGCPEWLQPTYNLETHLSQLIGDYLVRKRDGLNN 701

Query: 704 LWILKPWNMARTIDTTVTDNLSAIIRLMETGPKICQKYIEHPALFNGKKFDLRYIVLVRS 763
           LWILKPWNMARTIDTTVTDNLSAIIRLMETGPKICQKYIEHPALFNGKKFDLRYIVLVRS
Sbjct: 702 LWILKPWNMARTIDTTVTDNLSAIIRLMETGPKICQKYIEHPALFNGKKFDLRYIVLVRS 761

Query: 764 MKPLEIFLADSFWVRLANNPYSLETQSLFEYETHFTVMFYLAVSVACNSSWCAREIDYN- 823
           MKPLEIFLADSFWVRLANNPYSLE QSLFEYETHFTVM Y       N +   +E +   
Sbjct: 762 MKPLEIFLADSFWVRLANNPYSLEKQSLFEYETHFTVMNYRGKLNHKNIADFVKEFEQEH 821

Query: 824 --AWLDIHSRVRSMIRSVFESAMVVHPEMHSPFSRAIYGLDVMLDSSFQPKLLEVTYCPD 883
              WLDIHSRVRSMIRSVFESA VVHPEMHSPFSRA+YGLDVMLDSSFQPKLLEVTYCPD
Sbjct: 822 NVKWLDIHSRVRSMIRSVFESAAVVHPEMHSPFSRAMYGLDVMLDSSFQPKLLEVTYCPD 881

Query: 884 CTRACKYDVENVYGG-VIKGKEFYNDVFGCLFLNETTHVTPL 921
           CTRACKYDVENV+G  +IKG+EFYNDVFGCLFLNETTH TPL
Sbjct: 882 CTRACKYDVENVFGDEIIKGREFYNDVFGCLFLNETTHATPL 892

BLAST of Lsi06G003350 vs. TAIR 10
Match: AT1G77550.1 (tubulin-tyrosine ligases;tubulin-tyrosine ligases )

HSP 1 Score: 1115.9 bits (2885), Expect = 0.0e+00
Identity = 545/882 (61.79%), Postives = 680/882 (77.10%), Query Frame = 0

Query: 44  LRISSKFMVCSSLPLD-FPSLCTKLTSETFDGGAHFQVEPFEDGRCRRLVLSSDFMAKES 103
           +++    +  S LP   +P L  KL  +TFDGG +FQ+E  ED + R+L+L+++ M K+S
Sbjct: 11  VKVHGILLAASGLPPKLYPRLFEKLAYDTFDGGDYFQIESCEDDQRRKLLLTAESMPKDS 70

Query: 104 HVFLVDHAWTFRLSDAYTQLLEVPGLAERMASLMCVDVDMNLDEEEEDQSKSNDDEAKQS 163
            VFLVDHAWTFRL DAY QL E+PGLAERMASLM VD+D++  EEE  +  S D      
Sbjct: 71  DVFLVDHAWTFRLPDAYKQLKEIPGLAERMASLMSVDIDVDAGEEEVAEELSVD------ 130

Query: 164 VWELLESEIRGAKEKGNDSVRWLELEDLQIEDDTLLSLDLPTKFPDLLALSLTGNKLRNV 223
             +++++EIR A +KG DS+RWLELE L + DD+LLSL LP+KF DL+ALSL GNK+ + 
Sbjct: 131 --QIIDNEIRYAADKGYDSLRWLELEGLGV-DDSLLSLHLPSKFQDLVALSLIGNKIESA 190

Query: 224 DVIAQEVAKFKHLRALWLNDNPVAENWYFSELFLFSDENLQQKVLEGSPNLEIYNSRFTL 283
           DV+ QE+ KFK+L+ALWLNDNPV +          S+  +  ++L+G P+LEIYNS FT 
Sbjct: 191 DVVIQEIVKFKNLKALWLNDNPVLQK---------SERQMADEILQGCPSLEIYNSCFTP 250

Query: 284 NFSKWALGFCGDMYGKDNPGSIYPADHTLQCLTSLDLSSRYIHNLINKAFSPVELPSLLY 343
           N+  WALGFCGD++GKDNPG +   D  L  +TSLDLS+R IHNL+NKAFS  E+P L +
Sbjct: 251 NYGLWALGFCGDIFGKDNPGCV-QQDQPLCNVTSLDLSNRSIHNLVNKAFSVHEMPLLSH 310

Query: 344 LNLKGNPLEQNSVGDLLKVLKEFPCLSSLEVDIPGPLGEKASDIIESLPNLNALNGIDVA 403
           LN++GN L+QNSVG+LL+VLK FP LSSLEVDIPGPLG  A +I+ESL NL+ LNG+D A
Sbjct: 311 LNIRGNSLDQNSVGELLEVLKLFPSLSSLEVDIPGPLGINALEILESLSNLSLLNGVDTA 370

Query: 404 KILNSGKHVIDSMLLPRLPEWAPEEPLPDRVINAMWQYLMTYRLADEEKIDETSVWYAVY 463
           KIL +GKHV+DSML PR+PE  P++ L DRV++AMW Y + YRLAD+EK+DETS+W    
Sbjct: 371 KILETGKHVVDSMLQPRIPELNPDDTLVDRVLDAMWLYALNYRLADDEKLDETSLW---- 430

Query: 464 RLGLSYFSIFSRMYVMDELGSALRHSDEPNFRVAPFLFMPEGELTSAISFTILWPIHNVQ 523
                        YVMDELGSALRHSDEPNF+VAPFLFMP G+L SA+S++++WPI + Q
Sbjct: 431 -------------YVMDELGSALRHSDEPNFKVAPFLFMPSGKLESAVSYSVMWPIKHSQ 490

Query: 524 KGDECTRDYLFGFGEDKQRSARLTAWFHTPQNYFVHEYEKHIRNLQSKALASSISQTTSN 583
           KGDECTRD+L G GEDKQRSARLTAWF TP+NYF+HE+EK+ + LQ+KA  S  S   S 
Sbjct: 491 KGDECTRDFLSGIGEDKQRSARLTAWFQTPENYFIHEFEKYQQKLQAKAFESKPS-NPSV 550

Query: 584 PEGLHQSKGGTFHVYTDNPQVEEFLNRPEFIMTSDPKEADIIWTSMQIDEDTRKATGITD 643
              +  S G    VYTD PQVEEFL RPEF++T++PK+ADI+WTS+Q+D++ +K  GITD
Sbjct: 551 SRSIQHSDGSPLLVYTDLPQVEEFLTRPEFVITNEPKDADILWTSVQVDDELKKEVGITD 610

Query: 644 QQYVNQFPFEACLVMKHHLAETVEKAHGCPEWLQPTYNLETHLSQLIGDYLVRKRDGLNN 703
            QY+NQFPFEACLVMKHHLAET++  +G P+WLQPTYNLET LSQ IGDY VRKRD LNN
Sbjct: 611 DQYINQFPFEACLVMKHHLAETIQMGYGSPKWLQPTYNLETQLSQFIGDYCVRKRDQLNN 670

Query: 704 LWILKPWNMARTIDTTVTDNLSAIIRLMETGPKICQKYIEHPALFNGKKFDLRYIVLVRS 763
           LWILKPWNMARTIDT++TDNLSAIIR+METGPKICQKYIEHPALF G KFDLRY+VLVRS
Sbjct: 671 LWILKPWNMARTIDTSITDNLSAIIRMMETGPKICQKYIEHPALFKGNKFDLRYVVLVRS 730

Query: 764 MKPLEIFLADSFWVRLANNPYSLETQSLFEYETHFTVMFYLAVSVACNSSWCAREIDYN- 823
           + PLEI+L + FWVRL+NNPYSLE  S FEYETHFTVM Y        ++   RE +   
Sbjct: 731 IDPLEIYLIEIFWVRLSNNPYSLEKHSFFEYETHFTVMNYGRKLNHKPTAEFVREFEQEH 790

Query: 824 --AWLDIHSRVRSMIRSVFESAMVVHPEMHSPFSRAIYGLDVMLDSSFQPKLLEVTYCPD 883
              W+DIH +V+ +IR+VFE+A + HPEM SP SRA+YG+DVMLDSSF+PK+LEVTYCPD
Sbjct: 791 NVKWMDIHEKVKQVIRAVFEAAALAHPEMQSPKSRAMYGVDVMLDSSFEPKILEVTYCPD 850

Query: 884 CTRACKYDVENVYG-GVIKGKEFYNDVFGCLFLNETTHVTPL 921
           C RACKYD+E + G G++KG +F+N+VFGCLFL+ET HVTPL
Sbjct: 851 CMRACKYDMETIDGKGIVKGGDFFNNVFGCLFLDETAHVTPL 855

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q3UDE21.4e-7734.50Tubulin--tyrosine ligase-like protein 12 OS=Mus musculus OX=10090 GN=Ttll12 PE=1... [more]
Q141662.8e-7334.63Tubulin--tyrosine ligase-like protein 12 OS=Homo sapiens OX=9606 GN=TTLL12 PE=1 ... [more]
Q095121.4e-6131.16Tubulin--tyrosine ligase-like protein 12 OS=Caenorhabditis elegans OX=6239 GN=tt... [more]
A8XXC02.7e-6031.30Tubulin--tyrosine ligase-like protein 12 OS=Caenorhabditis briggsae OX=6238 GN=t... [more]
Q9VM913.0e-1125.66Tubulin glycylase 3A OS=Drosophila melanogaster OX=7227 GN=TTLL3A PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A5D3CI020.0e+0085.84Tubulin--tyrosine ligase-like protein 12 OS=Cucumis melo var. makuwa OX=1194695 ... [more]
A0A5A7UQ890.0e+0085.73Tubulin--tyrosine ligase-like protein 12 OS=Cucumis melo var. makuwa OX=1194695 ... [more]
A0A1S3BR240.0e+0085.73tubulin--tyrosine ligase-like protein 12 OS=Cucumis melo OX=3656 GN=LOC103492368... [more]
A0A0A0LWN50.0e+0085.46Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G596500 PE=4 SV=1[more]
A0A6J1F3Y40.0e+0084.13tubulin--tyrosine ligase-like protein 12 OS=Cucurbita moschata OX=3662 GN=LOC111... [more]
Match NameE-valueIdentityDescription
XP_038879092.10.0e+0086.86tubulin--tyrosine ligase-like protein 12 [Benincasa hispida][more]
TYK10026.10.0e+0085.84tubulin--tyrosine ligase-like protein 12 [Cucumis melo var. makuwa][more]
XP_008450923.10.0e+0085.73PREDICTED: tubulin--tyrosine ligase-like protein 12 [Cucumis melo] >KAA0055775.1... [more]
XP_004144031.10.0e+0085.46tubulin--tyrosine ligase-like protein 12 [Cucumis sativus] >KGN66300.1 hypotheti... [more]
KAG6588082.10.0e+0084.24Tubulin--tyrosine ligase-like protein 12, partial [Cucurbita argyrosperma subsp.... [more]
Match NameE-valueIdentityDescription
AT1G77550.10.0e+0061.79tubulin-tyrosine ligases;tubulin-tyrosine ligases [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR004344Tubulin-tyrosine ligase/Tubulin polyglutamylasePFAMPF03133TTLcoord: 643..889
e-value: 2.4E-47
score: 161.5
IPR004344Tubulin-tyrosine ligase/Tubulin polyglutamylasePROSITEPS51221TTLcoord: 589..914
score: 30.362619
NoneNo IPR availableGENE3D3.30.470.20coord: 715..901
e-value: 1.9E-30
score: 108.0
NoneNo IPR availableSUPERFAMILY56059Glutathione synthetase ATP-binding domain-likecoord: 700..880
NoneNo IPR availableSUPERFAMILY52047RNI-likecoord: 181..396
IPR032675Leucine-rich repeat domain superfamilyGENE3D3.80.10.10Ribonuclease Inhibitorcoord: 128..281
e-value: 1.9E-12
score: 48.7
IPR032675Leucine-rich repeat domain superfamilyGENE3D3.80.10.10Ribonuclease Inhibitorcoord: 302..404
e-value: 1.0E-6
score: 29.9
IPR027749Tubulin--tyrosine ligase-like protein 12PANTHERPTHR46088TUBULIN--TYROSINE LIGASE-LIKE PROTEIN 12coord: 61..919

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi06G003350.1Lsi06G003350.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006464 cellular protein modification process
molecular_function GO:0016874 ligase activity