Lsi05G015650 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi05G015650
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionChaperone protein DnaJ
Locationchr05: 23415577 .. 23416794 (+)
RNA-Seq ExpressionLsi05G015650
SyntenyLsi05G015650
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGTCCTGACTCCGATTTCAAATCCCATTTAGCCTCTGAAATCTGCTCCTTCTCCTCCCGTACTGTCTCTTGCCCTCACCGCCATTTCTCCCTCCCTTTCGTCGACTGGTATCGCCTTTTTCGGGTATGTTTTTCCCCTTCCTCTTATGGGTTTTCTTTCTTTTCTTCCCTTCTCTCTTAATCCATTCTCATAGGTCGAACAAGACGCTGGAATCGATTCCATTCGTAGGCGATATCTCAAGCTTGGTGGGTTTCTCTTTTCTTCCTCTCTCCATTCCCATTTCCTTTTGTTTTGATCAGAAAATGTGCTTTGGATTTGCAGCTCTGCAACTTCATCCAGATAAAAACTCACACCCCAAGGCCGAAATCGCCTTCAAGCTTGTCTCTGAGGTTCGATTCCATTCATTTTCTTGGAAACCCAACTTTCTGCCTTATATCTGTTCTACATTTTTGTGATTTTGCTTTTTGAGTGTTTTTTCCATGACGATCCATTACTGTAAATCTTGCAGTTAATTTCTGTATTCATGTACTACTCGTGAATCTTGATGAATGTTGCTATAATGAAACTTTGTGTTTTTTCACTTTATTGCAAAATAAATTCTGAAGGATGAGAAATGTGAATGTGAATGAGGGATTGTTTTGTTTGTGTTTGTAGGGATATGGTTGTTTGAGTGATAATGTGAAGAGAAGGGAATTTGATTTGGATAGGAAGAAGAAGTTTTGCCCTGATTGCAACACAATCCCTTATAGAAATTTCAGCTCTGCTAGAAACTTGAATGCCTTGAATGTATTAGGAAGTTTTCACAACAACAACAACAACAACATCCATGGAGGTTTGAGAGATATGAAAGAGAGATTGAGAGAGGAAGCTAATGTGATTGAGAATTGTTTGAGGGCTAATAATTCTGCAGCAAACAGAGAAACTTCACTATTCAATCCACCTGGTTTCAACATGTTTCATTACAGAAACCACAAAGAAACCCCAATCTTCAATCCTTCTGATTATGTTTGTCAAGGCTACCCCCATTTCCGAACCCGAATCCACCCGAAACCGCAGAGTTTTTGTTGTTTTAGAACTGGAAATGCATTGAAGTATGAACAAGCTAAAGGGAATTATGAGTATCCAGTGTTTGAGATTAGTAGATCAGAAAGTTTTCTTTTACAGAAACAATCTCCTTTTGTTTATTCAAGTGTTAACAAATCATCACATTGGTGA

mRNA sequence

ATGGGTCCTGACTCCGATTTCAAATCCCATTTAGCCTCTGAAATCTGCTCCTTCTCCTCCCGTACTGTCTCTTGCCCTCACCGCCATTTCTCCCTCCCTTTCGTCGACTGGTATCGCCTTTTTCGGGTCGAACAAGACGCTGGAATCGATTCCATTCGTAGGCGATATCTCAAGCTTGCTCTGCAACTTCATCCAGATAAAAACTCACACCCCAAGGCCGAAATCGCCTTCAAGCTTGTCTCTGAGGGATATGGTTGTTTGAGTGATAATGTGAAGAGAAGGGAATTTGATTTGGATAGGAAGAAGAAGTTTTGCCCTGATTGCAACACAATCCCTTATAGAAATTTCAGCTCTGCTAGAAACTTGAATGCCTTGAATGTATTAGGAAGTTTTCACAACAACAACAACAACAACATCCATGGAGGTTTGAGAGATATGAAAGAGAGATTGAGAGAGGAAGCTAATGTGATTGAGAATTGTTTGAGGGCTAATAATTCTGCAGCAAACAGAGAAACTTCACTATTCAATCCACCTGGTTTCAACATGTTTCATTACAGAAACCACAAAGAAACCCCAATCTTCAATCCTTCTGATTATGTTTGTCAAGGCTACCCCCATTTCCGAACCCGAATCCACCCGAAACCGCAGAGTTTTTGTTGTTTTAGAACTGGAAATGCATTGAAGTATGAACAAGCTAAAGGGAATTATGAGTATCCAGTGTTTGAGATTAGTAGATCAGAAAGTTTTCTTTTACAGAAACAATCTCCTTTTGTTTATTCAAGTGTTAACAAATCATCACATTGGTGA

Coding sequence (CDS)

ATGGGTCCTGACTCCGATTTCAAATCCCATTTAGCCTCTGAAATCTGCTCCTTCTCCTCCCGTACTGTCTCTTGCCCTCACCGCCATTTCTCCCTCCCTTTCGTCGACTGGTATCGCCTTTTTCGGGTCGAACAAGACGCTGGAATCGATTCCATTCGTAGGCGATATCTCAAGCTTGCTCTGCAACTTCATCCAGATAAAAACTCACACCCCAAGGCCGAAATCGCCTTCAAGCTTGTCTCTGAGGGATATGGTTGTTTGAGTGATAATGTGAAGAGAAGGGAATTTGATTTGGATAGGAAGAAGAAGTTTTGCCCTGATTGCAACACAATCCCTTATAGAAATTTCAGCTCTGCTAGAAACTTGAATGCCTTGAATGTATTAGGAAGTTTTCACAACAACAACAACAACAACATCCATGGAGGTTTGAGAGATATGAAAGAGAGATTGAGAGAGGAAGCTAATGTGATTGAGAATTGTTTGAGGGCTAATAATTCTGCAGCAAACAGAGAAACTTCACTATTCAATCCACCTGGTTTCAACATGTTTCATTACAGAAACCACAAAGAAACCCCAATCTTCAATCCTTCTGATTATGTTTGTCAAGGCTACCCCCATTTCCGAACCCGAATCCACCCGAAACCGCAGAGTTTTTGTTGTTTTAGAACTGGAAATGCATTGAAGTATGAACAAGCTAAAGGGAATTATGAGTATCCAGTGTTTGAGATTAGTAGATCAGAAAGTTTTCTTTTACAGAAACAATCTCCTTTTGTTTATTCAAGTGTTAACAAATCATCACATTGGTGA

Protein sequence

MGPDSDFKSHLASEICSFSSRTVSCPHRHFSLPFVDWYRLFRVEQDAGIDSIRRRYLKLALQLHPDKNSHPKAEIAFKLVSEGYGCLSDNVKRREFDLDRKKKFCPDCNTIPYRNFSSARNLNALNVLGSFHNNNNNNIHGGLRDMKERLREEANVIENCLRANNSAANRETSLFNPPGFNMFHYRNHKETPIFNPSDYVCQGYPHFRTRIHPKPQSFCCFRTGNALKYEQAKGNYEYPVFEISRSESFLLQKQSPFVYSSVNKSSHW
Homology
BLAST of Lsi05G015650 vs. ExPASy Swiss-Prot
Match: Q9FH28 (Chaperone protein dnaJ 49 OS=Arabidopsis thaliana OX=3702 GN=ATJ49 PE=2 SV=2)

HSP 1 Score: 70.9 bits (172), Expect = 2.5e-11
Identity = 30/62 (48.39%), Postives = 44/62 (70.97%), Query Frame = 0

Query: 36  DWYRLFRVEQDAGIDSIRRRYLKLALQLHPDKNSHPKAEIAFKLVSEGYGCLSDNVKRRE 95
           D+Y +  +E++  +D IR+ Y KL+L++HPDKN  P +E AFK VS+ + CLSD   RR+
Sbjct: 99  DYYAILGLEKNCSVDEIRKAYRKLSLKVHPDKNKAPGSEEAFKKVSKAFTCLSDGNSRRQ 158

Query: 96  FD 98
           FD
Sbjct: 159 FD 160

BLAST of Lsi05G015650 vs. ExPASy Swiss-Prot
Match: Q8TQR1 (Chaperone protein DnaJ OS=Methanosarcina acetivorans (strain ATCC 35395 / DSM 2834 / JCM 12185 / C2A) OX=188937 GN=dnaJ PE=3 SV=1)

HSP 1 Score: 68.2 bits (165), Expect = 1.6e-10
Identity = 29/62 (46.77%), Postives = 41/62 (66.13%), Query Frame = 0

Query: 36 DWYRLFRVEQDAGIDSIRRRYLKLALQLHPDKNSHPKAEIAFKLVSEGYGCLSDNVKRRE 95
          D+Y +  + +DA ++ I++ Y KLALQ HPD+N  P AE  FK +SE Y  LSD  KR +
Sbjct: 6  DYYEILGLPKDASVEDIKKTYRKLALQYHPDRNKDPGAEDKFKEISEAYAVLSDTEKRAQ 65

Query: 96 FD 98
          +D
Sbjct: 66 YD 67

BLAST of Lsi05G015650 vs. ExPASy Swiss-Prot
Match: P0CW07 (Chaperone protein DnaJ OS=Methanosarcina mazei (strain ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11833 / OCM 88) OX=192952 GN=dnaJ PE=3 SV=1)

HSP 1 Score: 67.0 bits (162), Expect = 3.7e-10
Identity = 28/62 (45.16%), Postives = 41/62 (66.13%), Query Frame = 0

Query: 36 DWYRLFRVEQDAGIDSIRRRYLKLALQLHPDKNSHPKAEIAFKLVSEGYGCLSDNVKRRE 95
          D+Y +  + +D+ ++ I++ Y KLALQ HPD+N  P AE  FK +SE Y  LSD  KR +
Sbjct: 6  DYYEILGLSKDSSVEDIKKTYRKLALQYHPDRNKEPGAEEKFKEISEAYAVLSDAEKRAQ 65

Query: 96 FD 98
          +D
Sbjct: 66 YD 67

BLAST of Lsi05G015650 vs. ExPASy Swiss-Prot
Match: P0CW06 (Chaperone protein DnaJ OS=Methanosarcina mazei OX=2209 GN=dnaJ PE=3 SV=1)

HSP 1 Score: 67.0 bits (162), Expect = 3.7e-10
Identity = 28/62 (45.16%), Postives = 41/62 (66.13%), Query Frame = 0

Query: 36 DWYRLFRVEQDAGIDSIRRRYLKLALQLHPDKNSHPKAEIAFKLVSEGYGCLSDNVKRRE 95
          D+Y +  + +D+ ++ I++ Y KLALQ HPD+N  P AE  FK +SE Y  LSD  KR +
Sbjct: 6  DYYEILGLSKDSSVEDIKKTYRKLALQYHPDRNKEPGAEEKFKEISEAYAVLSDAEKRAQ 65

Query: 96 FD 98
          +D
Sbjct: 66 YD 67

BLAST of Lsi05G015650 vs. ExPASy Swiss-Prot
Match: Q17438 (DnaJ homolog subfamily B member 1 OS=Caenorhabditis elegans OX=6239 GN=dnj-1 PE=1 SV=1)

HSP 1 Score: 65.5 bits (158), Expect = 1.1e-09
Identity = 29/62 (46.77%), Postives = 40/62 (64.52%), Query Frame = 0

Query: 36  DWYRLFRVEQDAGIDSIRRRYLKLALQLHPDKNSHPKAEIAFKLVSEGYGCLSDNVKRRE 95
           D+Y + ++++ A  D IR+ Y KLAL+LHPDK   P A  AFK +   Y  LSD  KRR+
Sbjct: 137 DYYEILKIDKKASDDDIRKEYRKLALKLHPDKCRAPHATEAFKALGNAYAVLSDTDKRRQ 196

Query: 96  FD 98
           +D
Sbjct: 197 YD 198

BLAST of Lsi05G015650 vs. ExPASy TrEMBL
Match: A0A5D3CXW5 (Chaperone protein DnaJ OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold209G001280 PE=4 SV=1)

HSP 1 Score: 475.3 bits (1222), Expect = 1.7e-130
Identity = 231/270 (85.56%), Postives = 244/270 (90.37%), Query Frame = 0

Query: 1   MGPDSDFKSHLASEICSFSSRTVSCPHRHFSLPFVDWYRLFRVEQDAGIDSIRRRYLKLA 60
           MGPDSDFKSHLA EI SFSSRTVSCPHRHFS PF+DWYRLF V+QDA ID IR RYLKLA
Sbjct: 1   MGPDSDFKSHLAYEISSFSSRTVSCPHRHFSPPFIDWYRLFGVQQDAPIDFIRSRYLKLA 60

Query: 61  LQLHPDKNSHPKAEIAFKLVSEGYGCLSDNVKRREFDLDRKKKFCPDCNTIPYRNFSSAR 120
           LQLHPDKNSHPKAEIAFKLVSEGYGCLSDNVKRR FDLDRK KFCPDCNTIPYRNF+SA 
Sbjct: 61  LQLHPDKNSHPKAEIAFKLVSEGYGCLSDNVKRRAFDLDRKNKFCPDCNTIPYRNFTSAT 120

Query: 121 NLNALNVLGSFHNNNNNNIH-GGLRDMKERLREEANVIENCLRANNSAANRETSLFNPPG 180
           NLNALN+ G FH    NNIH GGLRDMKERLREEANVIENCLRANNS++  ETSLFNPPG
Sbjct: 121 NLNALNIFGGFH----NNIHGGGLRDMKERLREEANVIENCLRANNSSSTTETSLFNPPG 180

Query: 181 FNMFHYRNHKETPIFNPSDYVCQGYPHFRTRIHPKPQSFCCFRTGNALKYEQAKGNYEYP 240
            NMFH+RN+KETPIFNPSDYVCQGYPHFRTRIHPK +SF CFR+GNALKYEQ +G YEYP
Sbjct: 181 CNMFHHRNYKETPIFNPSDYVCQGYPHFRTRIHPKARSFRCFRSGNALKYEQGRGKYEYP 240

Query: 241 VFEI-SRSESFLLQKQSPFVYSSVNKSSHW 269
           VFEI S+SESFLLQKQS FVYS+VNKSSHW
Sbjct: 241 VFEISSKSESFLLQKQSAFVYSNVNKSSHW 266

BLAST of Lsi05G015650 vs. ExPASy TrEMBL
Match: A0A5A7U7H9 (Chaperone protein DnaJ OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold171G007750 PE=4 SV=1)

HSP 1 Score: 473.8 bits (1218), Expect = 4.8e-130
Identity = 230/270 (85.19%), Postives = 243/270 (90.00%), Query Frame = 0

Query: 1   MGPDSDFKSHLASEICSFSSRTVSCPHRHFSLPFVDWYRLFRVEQDAGIDSIRRRYLKLA 60
           MGPDSDFKSHL  EI SFSSRTVSCPHRHFS PF+DWYRLF V+QDA ID IR RYLKLA
Sbjct: 1   MGPDSDFKSHLTYEISSFSSRTVSCPHRHFSPPFIDWYRLFGVQQDAPIDFIRSRYLKLA 60

Query: 61  LQLHPDKNSHPKAEIAFKLVSEGYGCLSDNVKRREFDLDRKKKFCPDCNTIPYRNFSSAR 120
           LQLHPDKNSHPKAEIAFKLVSEGYGCLSDNVKRR FDLDRK KFCPDCNTIPYRNF+SA 
Sbjct: 61  LQLHPDKNSHPKAEIAFKLVSEGYGCLSDNVKRRAFDLDRKNKFCPDCNTIPYRNFTSAT 120

Query: 121 NLNALNVLGSFHNNNNNNIH-GGLRDMKERLREEANVIENCLRANNSAANRETSLFNPPG 180
           NLNALN+ G FH    NNIH GGLRDMKERLREEANVIENCLRANNS++  ETSLFNPPG
Sbjct: 121 NLNALNIFGGFH----NNIHGGGLRDMKERLREEANVIENCLRANNSSSTTETSLFNPPG 180

Query: 181 FNMFHYRNHKETPIFNPSDYVCQGYPHFRTRIHPKPQSFCCFRTGNALKYEQAKGNYEYP 240
            NMFH+RN+KETPIFNPSDYVCQGYPHFRTRIHPK +SF CFR+GNALKYEQ +G YEYP
Sbjct: 181 CNMFHHRNYKETPIFNPSDYVCQGYPHFRTRIHPKARSFRCFRSGNALKYEQGRGKYEYP 240

Query: 241 VFEI-SRSESFLLQKQSPFVYSSVNKSSHW 269
           VFEI S+SESFLLQKQS FVYS+VNKSSHW
Sbjct: 241 VFEISSKSESFLLQKQSAFVYSNVNKSSHW 266

BLAST of Lsi05G015650 vs. ExPASy TrEMBL
Match: A0A1S3AXH7 (chaperone protein DnaJ OS=Cucumis melo OX=3656 GN=LOC103483872 PE=4 SV=1)

HSP 1 Score: 473.4 bits (1217), Expect = 6.3e-130
Identity = 231/271 (85.24%), Postives = 243/271 (89.67%), Query Frame = 0

Query: 1   MGPDSDFKSHLASEICSFSSRTVSCPHRHFSLPFVDWYRLFRVEQDAGIDSIRRRYLKLA 60
           MGPDSDFKSHLA EI SFSS TVSCPHRHFS PF+DWYRLF V+QDA ID IR RYLKLA
Sbjct: 1   MGPDSDFKSHLAYEISSFSSLTVSCPHRHFSPPFIDWYRLFGVQQDAPIDFIRSRYLKLA 60

Query: 61  LQLHPDKNSHPKAEIAFKLVSEGYGCLSDNVKRREFDLDRKKKFCPDCNTIPYRNFSSAR 120
           LQLHPDKNSHPKAEIAFKLVSEGYGCLSDNVKRR FDLDRK KFCPDCNTIPYRNF+SA 
Sbjct: 61  LQLHPDKNSHPKAEIAFKLVSEGYGCLSDNVKRRAFDLDRKNKFCPDCNTIPYRNFTSAT 120

Query: 121 NLNALNVLGSFHNNNNNNIH-GGLRDMKERLREEANVIENCLRANNSAANRETSLFNPPG 180
           NLNALN+ G FH    NNIH GGLRDMKERLREEANVIENCLRANNS++  ETSLFNPPG
Sbjct: 121 NLNALNIFGGFH----NNIHGGGLRDMKERLREEANVIENCLRANNSSSTTETSLFNPPG 180

Query: 181 FNMFHYRNHKETPIFNPSDYVCQGYPHFRTRIHPKPQSFCCFRTGNALKYEQAKGNYEYP 240
            NMFH+RN+KETPIFNPSDYVCQGYPHFRTRIHPK +SF CFR+GNALKYEQ +G YEYP
Sbjct: 181 CNMFHHRNYKETPIFNPSDYVCQGYPHFRTRIHPKARSFRCFRSGNALKYEQGRGKYEYP 240

Query: 241 VFEI--SRSESFLLQKQSPFVYSSVNKSSHW 269
           VFEI  SRSESFLLQKQS FVYS+VNKSSHW
Sbjct: 241 VFEISSSRSESFLLQKQSAFVYSNVNKSSHW 267

BLAST of Lsi05G015650 vs. ExPASy TrEMBL
Match: A0A0A0L5B2 (J domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G165080 PE=4 SV=1)

HSP 1 Score: 461.8 bits (1187), Expect = 1.9e-126
Identity = 229/271 (84.50%), Postives = 241/271 (88.93%), Query Frame = 0

Query: 1   MGPDSDFKSHLASEICSFSSRTVSCPHRHFSLPFVDWYRLFRVEQDAGIDSIRRRYLKLA 60
           MGP SDFKSHLASEI SFSS TVSCPHR FS PF+DWYRLF V+QDA ID IR RYLKLA
Sbjct: 1   MGPHSDFKSHLASEISSFSSLTVSCPHRLFSPPFIDWYRLFGVQQDAPIDFIRSRYLKLA 60

Query: 61  LQLHPDKNSHPKAEIAFKLVSEGYGCLSDNVKRREFDLDRKKKFCPDCNTIPYRNFSSAR 120
           LQLHPDKNSHPKAEIAFKLVSEGYGCLSDNVKRR FDLDRK KFC DCNTIPYRN++SA 
Sbjct: 61  LQLHPDKNSHPKAEIAFKLVSEGYGCLSDNVKRRAFDLDRKDKFCADCNTIPYRNYTSAT 120

Query: 121 NLNALNVLGSFHNNNNNNIHG-GLRDMKERLREEANVIENCLRANNSAANRETSLFNPPG 180
           NLNALNVLGSFH    NNIHG GLRD+KERLREEANVIE CLRANNS+   ETSLFNPPG
Sbjct: 121 NLNALNVLGSFH----NNIHGRGLRDVKERLREEANVIEYCLRANNSSTTTETSLFNPPG 180

Query: 181 FNMFHYRNHKETPIFNPSDYVCQGYPHFRTRIHPKPQSFCCFRTGNALKYEQAKGNYEYP 240
            +MFH+RN+KETPIFNPSDYVCQGYPHFRTRIHPKPQSF CFR+GNALKYEQ +G  EYP
Sbjct: 181 CSMFHHRNYKETPIFNPSDYVCQGYPHFRTRIHPKPQSFRCFRSGNALKYEQGRGKCEYP 240

Query: 241 VFEIS--RSESFLLQKQSPFVYSSVNKSSHW 269
           VFEIS  RSESFLLQKQS FVYS+VNKSSHW
Sbjct: 241 VFEISSNRSESFLLQKQSAFVYSNVNKSSHW 267

BLAST of Lsi05G015650 vs. ExPASy TrEMBL
Match: A0A6J1IXP0 (dnaJ homolog subfamily B member 9-like OS=Cucurbita maxima OX=3661 GN=LOC111479463 PE=4 SV=1)

HSP 1 Score: 380.2 bits (975), Expect = 7.2e-102
Identity = 188/271 (69.37%), Postives = 206/271 (76.01%), Query Frame = 0

Query: 1   MGPDSDFKSHLASEICSFSSRTVSCPHRHFSLPFVDWYRLFRVEQDAGIDSIRRRYLKLA 60
           M PDSDFKS LA+EICSFS+R+VSCPHRHFS PF+DWYRLF VEQDAGIDSIRRRYLKLA
Sbjct: 1   MAPDSDFKSDLAAEICSFSARSVSCPHRHFSRPFLDWYRLFGVEQDAGIDSIRRRYLKLA 60

Query: 61  LQLHPDKNSHPKAEIAFKLVSEGYGCLSDNVKRREFDLDRKKKFCPDCNTIPYRNFSSAR 120
           LQLHPDKN+HPKAEIAFKLVSEGYGCL DNVKRR FDLDR KKFCPDCNTIPYRN SSAR
Sbjct: 61  LQLHPDKNAHPKAEIAFKLVSEGYGCLIDNVKRRAFDLDRMKKFCPDCNTIPYRNLSSAR 120

Query: 121 NLNALNVLGSFHNNNNNNIHGGLRDMKERLREEANVIENCLRANNSAANRETSLFNPP-- 180
           N N L                             N+ ENCLRANNS    +    +P   
Sbjct: 121 NSNPL-----------------------------NLFENCLRANNSREFSQQFNNSPDCL 180

Query: 181 -GFNMFHYRNHKETPIFNPSDYVCQGYPHFRTRIHPKPQSFCCFRTGNALKYEQAKGNYE 240
                FH+RNHKE+PIFNPSDY CQGYPHFRT+IHPKPQS+ CFRTGN+LKYEQ +G YE
Sbjct: 181 INNGRFHHRNHKESPIFNPSDYACQGYPHFRTQIHPKPQSYRCFRTGNSLKYEQGRGKYE 240

Query: 241 YPVFEISRSESFLLQKQSPFVYSSVNKSSHW 269
           YPVFE+SRSE F+LQ+QS FV S+VNKS  W
Sbjct: 241 YPVFEMSRSERFVLQQQSAFVCSNVNKSHWW 242

BLAST of Lsi05G015650 vs. NCBI nr
Match: XP_038902437.1 (uncharacterized protein LOC120089081 [Benincasa hispida])

HSP 1 Score: 503.1 bits (1294), Expect = 1.5e-138
Identity = 244/269 (90.71%), Postives = 252/269 (93.68%), Query Frame = 0

Query: 1   MGPDSDFKSHLASEICSFSSRTVSCPHRHFSLPFVDWYRLFRVEQDAGIDSIRRRYLKLA 60
           MGPDSDFKSHLA+EI SFSSRTVSC HRHFSLPF+DWYRLF V+QDAGIDSIRRRYLKLA
Sbjct: 1   MGPDSDFKSHLATEISSFSSRTVSCSHRHFSLPFIDWYRLFGVQQDAGIDSIRRRYLKLA 60

Query: 61  LQLHPDKNSHPKAEIAFKLVSEGYGCLSDNVKRREFDLDRKKKFCPDCNTIPYRNFSSAR 120
           LQLHPDKNSHPKAEIAFKLVSEGYGCLSDNVKRREFDLDRKKKFCPDCNTIPYRNF+SAR
Sbjct: 61  LQLHPDKNSHPKAEIAFKLVSEGYGCLSDNVKRREFDLDRKKKFCPDCNTIPYRNFTSAR 120

Query: 121 NLNALNVLGSFHNNNN-NNIHGGLRDMKERLREEANVIENCLRANNSAANRETSLFNPPG 180
           N   LNVLG FHNNNN NNIHG LRDMKERLREEANVI+NCLR NNSAANRETSLFNPPG
Sbjct: 121 N---LNVLGGFHNNNNINNIHGDLRDMKERLREEANVIKNCLRTNNSAANRETSLFNPPG 180

Query: 181 FNMFHYRNHKETPIFNPSDYVCQGYPHFRTRIHPKPQSFCCFRTGNALKYEQAKGNYEYP 240
           F MFHY+NHKETPIFNPSDYVCQGYPHFRTRIHPKPQSF CFRTGNALKYEQ +G YEYP
Sbjct: 181 FTMFHYKNHKETPIFNPSDYVCQGYPHFRTRIHPKPQSFRCFRTGNALKYEQGRGKYEYP 240

Query: 241 VFEISRSESFLLQKQSPFVYSSVNKSSHW 269
           VFEISRSESFLLQKQS FVYS+VNK  HW
Sbjct: 241 VFEISRSESFLLQKQSAFVYSNVNK-PHW 265

BLAST of Lsi05G015650 vs. NCBI nr
Match: TYK16200.1 (chaperone protein DnaJ [Cucumis melo var. makuwa])

HSP 1 Score: 475.3 bits (1222), Expect = 3.4e-130
Identity = 231/270 (85.56%), Postives = 244/270 (90.37%), Query Frame = 0

Query: 1   MGPDSDFKSHLASEICSFSSRTVSCPHRHFSLPFVDWYRLFRVEQDAGIDSIRRRYLKLA 60
           MGPDSDFKSHLA EI SFSSRTVSCPHRHFS PF+DWYRLF V+QDA ID IR RYLKLA
Sbjct: 1   MGPDSDFKSHLAYEISSFSSRTVSCPHRHFSPPFIDWYRLFGVQQDAPIDFIRSRYLKLA 60

Query: 61  LQLHPDKNSHPKAEIAFKLVSEGYGCLSDNVKRREFDLDRKKKFCPDCNTIPYRNFSSAR 120
           LQLHPDKNSHPKAEIAFKLVSEGYGCLSDNVKRR FDLDRK KFCPDCNTIPYRNF+SA 
Sbjct: 61  LQLHPDKNSHPKAEIAFKLVSEGYGCLSDNVKRRAFDLDRKNKFCPDCNTIPYRNFTSAT 120

Query: 121 NLNALNVLGSFHNNNNNNIH-GGLRDMKERLREEANVIENCLRANNSAANRETSLFNPPG 180
           NLNALN+ G FH    NNIH GGLRDMKERLREEANVIENCLRANNS++  ETSLFNPPG
Sbjct: 121 NLNALNIFGGFH----NNIHGGGLRDMKERLREEANVIENCLRANNSSSTTETSLFNPPG 180

Query: 181 FNMFHYRNHKETPIFNPSDYVCQGYPHFRTRIHPKPQSFCCFRTGNALKYEQAKGNYEYP 240
            NMFH+RN+KETPIFNPSDYVCQGYPHFRTRIHPK +SF CFR+GNALKYEQ +G YEYP
Sbjct: 181 CNMFHHRNYKETPIFNPSDYVCQGYPHFRTRIHPKARSFRCFRSGNALKYEQGRGKYEYP 240

Query: 241 VFEI-SRSESFLLQKQSPFVYSSVNKSSHW 269
           VFEI S+SESFLLQKQS FVYS+VNKSSHW
Sbjct: 241 VFEISSKSESFLLQKQSAFVYSNVNKSSHW 266

BLAST of Lsi05G015650 vs. NCBI nr
Match: KAA0049521.1 (chaperone protein DnaJ [Cucumis melo var. makuwa])

HSP 1 Score: 473.8 bits (1218), Expect = 9.9e-130
Identity = 230/270 (85.19%), Postives = 243/270 (90.00%), Query Frame = 0

Query: 1   MGPDSDFKSHLASEICSFSSRTVSCPHRHFSLPFVDWYRLFRVEQDAGIDSIRRRYLKLA 60
           MGPDSDFKSHL  EI SFSSRTVSCPHRHFS PF+DWYRLF V+QDA ID IR RYLKLA
Sbjct: 1   MGPDSDFKSHLTYEISSFSSRTVSCPHRHFSPPFIDWYRLFGVQQDAPIDFIRSRYLKLA 60

Query: 61  LQLHPDKNSHPKAEIAFKLVSEGYGCLSDNVKRREFDLDRKKKFCPDCNTIPYRNFSSAR 120
           LQLHPDKNSHPKAEIAFKLVSEGYGCLSDNVKRR FDLDRK KFCPDCNTIPYRNF+SA 
Sbjct: 61  LQLHPDKNSHPKAEIAFKLVSEGYGCLSDNVKRRAFDLDRKNKFCPDCNTIPYRNFTSAT 120

Query: 121 NLNALNVLGSFHNNNNNNIH-GGLRDMKERLREEANVIENCLRANNSAANRETSLFNPPG 180
           NLNALN+ G FH    NNIH GGLRDMKERLREEANVIENCLRANNS++  ETSLFNPPG
Sbjct: 121 NLNALNIFGGFH----NNIHGGGLRDMKERLREEANVIENCLRANNSSSTTETSLFNPPG 180

Query: 181 FNMFHYRNHKETPIFNPSDYVCQGYPHFRTRIHPKPQSFCCFRTGNALKYEQAKGNYEYP 240
            NMFH+RN+KETPIFNPSDYVCQGYPHFRTRIHPK +SF CFR+GNALKYEQ +G YEYP
Sbjct: 181 CNMFHHRNYKETPIFNPSDYVCQGYPHFRTRIHPKARSFRCFRSGNALKYEQGRGKYEYP 240

Query: 241 VFEI-SRSESFLLQKQSPFVYSSVNKSSHW 269
           VFEI S+SESFLLQKQS FVYS+VNKSSHW
Sbjct: 241 VFEISSKSESFLLQKQSAFVYSNVNKSSHW 266

BLAST of Lsi05G015650 vs. NCBI nr
Match: XP_008438915.1 (PREDICTED: chaperone protein DnaJ [Cucumis melo])

HSP 1 Score: 473.4 bits (1217), Expect = 1.3e-129
Identity = 231/271 (85.24%), Postives = 243/271 (89.67%), Query Frame = 0

Query: 1   MGPDSDFKSHLASEICSFSSRTVSCPHRHFSLPFVDWYRLFRVEQDAGIDSIRRRYLKLA 60
           MGPDSDFKSHLA EI SFSS TVSCPHRHFS PF+DWYRLF V+QDA ID IR RYLKLA
Sbjct: 1   MGPDSDFKSHLAYEISSFSSLTVSCPHRHFSPPFIDWYRLFGVQQDAPIDFIRSRYLKLA 60

Query: 61  LQLHPDKNSHPKAEIAFKLVSEGYGCLSDNVKRREFDLDRKKKFCPDCNTIPYRNFSSAR 120
           LQLHPDKNSHPKAEIAFKLVSEGYGCLSDNVKRR FDLDRK KFCPDCNTIPYRNF+SA 
Sbjct: 61  LQLHPDKNSHPKAEIAFKLVSEGYGCLSDNVKRRAFDLDRKNKFCPDCNTIPYRNFTSAT 120

Query: 121 NLNALNVLGSFHNNNNNNIH-GGLRDMKERLREEANVIENCLRANNSAANRETSLFNPPG 180
           NLNALN+ G FH    NNIH GGLRDMKERLREEANVIENCLRANNS++  ETSLFNPPG
Sbjct: 121 NLNALNIFGGFH----NNIHGGGLRDMKERLREEANVIENCLRANNSSSTTETSLFNPPG 180

Query: 181 FNMFHYRNHKETPIFNPSDYVCQGYPHFRTRIHPKPQSFCCFRTGNALKYEQAKGNYEYP 240
            NMFH+RN+KETPIFNPSDYVCQGYPHFRTRIHPK +SF CFR+GNALKYEQ +G YEYP
Sbjct: 181 CNMFHHRNYKETPIFNPSDYVCQGYPHFRTRIHPKARSFRCFRSGNALKYEQGRGKYEYP 240

Query: 241 VFEI--SRSESFLLQKQSPFVYSSVNKSSHW 269
           VFEI  SRSESFLLQKQS FVYS+VNKSSHW
Sbjct: 241 VFEISSSRSESFLLQKQSAFVYSNVNKSSHW 267

BLAST of Lsi05G015650 vs. NCBI nr
Match: XP_004134496.2 (uncharacterized protein LOC101213407 [Cucumis sativus] >KGN57140.1 hypothetical protein Csa_010349 [Cucumis sativus])

HSP 1 Score: 461.8 bits (1187), Expect = 3.9e-126
Identity = 229/271 (84.50%), Postives = 241/271 (88.93%), Query Frame = 0

Query: 1   MGPDSDFKSHLASEICSFSSRTVSCPHRHFSLPFVDWYRLFRVEQDAGIDSIRRRYLKLA 60
           MGP SDFKSHLASEI SFSS TVSCPHR FS PF+DWYRLF V+QDA ID IR RYLKLA
Sbjct: 1   MGPHSDFKSHLASEISSFSSLTVSCPHRLFSPPFIDWYRLFGVQQDAPIDFIRSRYLKLA 60

Query: 61  LQLHPDKNSHPKAEIAFKLVSEGYGCLSDNVKRREFDLDRKKKFCPDCNTIPYRNFSSAR 120
           LQLHPDKNSHPKAEIAFKLVSEGYGCLSDNVKRR FDLDRK KFC DCNTIPYRN++SA 
Sbjct: 61  LQLHPDKNSHPKAEIAFKLVSEGYGCLSDNVKRRAFDLDRKDKFCADCNTIPYRNYTSAT 120

Query: 121 NLNALNVLGSFHNNNNNNIHG-GLRDMKERLREEANVIENCLRANNSAANRETSLFNPPG 180
           NLNALNVLGSFH    NNIHG GLRD+KERLREEANVIE CLRANNS+   ETSLFNPPG
Sbjct: 121 NLNALNVLGSFH----NNIHGRGLRDVKERLREEANVIEYCLRANNSSTTTETSLFNPPG 180

Query: 181 FNMFHYRNHKETPIFNPSDYVCQGYPHFRTRIHPKPQSFCCFRTGNALKYEQAKGNYEYP 240
            +MFH+RN+KETPIFNPSDYVCQGYPHFRTRIHPKPQSF CFR+GNALKYEQ +G  EYP
Sbjct: 181 CSMFHHRNYKETPIFNPSDYVCQGYPHFRTRIHPKPQSFRCFRSGNALKYEQGRGKCEYP 240

Query: 241 VFEIS--RSESFLLQKQSPFVYSSVNKSSHW 269
           VFEIS  RSESFLLQKQS FVYS+VNKSSHW
Sbjct: 241 VFEISSNRSESFLLQKQSAFVYSNVNKSSHW 267

BLAST of Lsi05G015650 vs. TAIR 10
Match: AT3G06778.1 (Chaperone DnaJ-domain superfamily protein )

HSP 1 Score: 156.4 bits (394), Expect = 3.3e-38
Identity = 85/213 (39.91%), Postives = 126/213 (59.15%), Query Frame = 0

Query: 3   PDSDFKSHLASEICSFSSRTVSCPHRH---FSLPFVDWYRLFRVEQDAGIDSIRRRYLKL 62
           P  D K+ L S+I + S   +SC H +       F+DWY +  +++DA +  IR+RY KL
Sbjct: 6   PGPDPKAALVSDILALSRSPISCIHINRISSGSCFIDWYLILGIQEDAEVKVIRKRYHKL 65

Query: 63  ALQLHPDKNSHPKAEIAFKLVSEGYGCLSDNVKRREFDLDRKKKFCPDCNTIPYRNFSSA 122
           AL++HPDKN+HPKA+IAFKL+ E Y CLSD  KRR F++DR+   C  C+ + ++   S 
Sbjct: 66  ALKVHPDKNNHPKADIAFKLIHEAYLCLSDETKRRSFNIDRRNNICLKCSRVSHK---SK 125

Query: 123 RNLNALNVLGSFHNNNNNNIHGGLRDMKERLREEANVIENCLRANNSAANRETSLFNPPG 182
            N N         ++  N     L+D++++ REE  VIE CL+ N++      +   PP 
Sbjct: 126 ENRN---------DSKPNRFCQTLKDIRDKFREENMVIERCLKTNSAFFMGNRTNETPPV 185

Query: 183 FNM-FHYRNHKETPIFNPSDYVCQGYPHFRTRI 212
           + +    R +KE+P+FNPSDY   GYPH R R+
Sbjct: 186 YGIPKQNRINKESPVFNPSDYRLWGYPHVRNRV 206

BLAST of Lsi05G015650 vs. TAIR 10
Match: AT5G18750.1 (DNAJ heat shock N-terminal domain-containing protein )

HSP 1 Score: 72.8 bits (177), Expect = 4.8e-13
Identity = 35/69 (50.72%), Postives = 45/69 (65.22%), Query Frame = 0

Query: 36  DWYRLFRVEQDAGIDSIRRRYLKLALQLHPDKNSHPKAEIAFKLVSEGYGCLSDNVKRRE 95
           DWY++ +VEQ A  ++I+++Y KLAL LHPDKN  P AE AFK + E    L D  KRR 
Sbjct: 66  DWYKILQVEQTADENTIKKQYKKLALHLHPDKNKLPGAESAFKTIGEAQRVLLDKDKRRF 125

Query: 96  FDLDRKKKF 105
            D+ RK  F
Sbjct: 126 HDMRRKPVF 134

BLAST of Lsi05G015650 vs. TAIR 10
Match: AT5G49060.1 (Heat shock protein DnaJ, N-terminal with domain of unknown function (DUF1977) )

HSP 1 Score: 70.9 bits (172), Expect = 1.8e-12
Identity = 30/62 (48.39%), Postives = 44/62 (70.97%), Query Frame = 0

Query: 36  DWYRLFRVEQDAGIDSIRRRYLKLALQLHPDKNSHPKAEIAFKLVSEGYGCLSDNVKRRE 95
           D+Y +  +E++  +D IR+ Y KL+L++HPDKN  P +E AFK VS+ + CLSD   RR+
Sbjct: 99  DYYAILGLEKNCSVDEIRKAYRKLSLKVHPDKNKAPGSEEAFKKVSKAFTCLSDGNSRRQ 158

Query: 96  FD 98
           FD
Sbjct: 159 FD 160

BLAST of Lsi05G015650 vs. TAIR 10
Match: AT3G04980.1 (DNAJ heat shock N-terminal domain-containing protein )

HSP 1 Score: 69.7 bits (169), Expect = 4.0e-12
Identity = 32/62 (51.61%), Postives = 42/62 (67.74%), Query Frame = 0

Query: 36  DWYRLFRVEQDAGIDSIRRRYLKLALQLHPDKNSHPKAEIAFKLVSEGYGCLSDNVKRRE 95
           DWY + +V+  A  D+I+++Y KLAL LHPDKN    AE AFKLV E    LSD +KR +
Sbjct: 48  DWYGVLQVQPYADADTIKKQYRKLALLLHPDKNKFAGAEAAFKLVGEANRLLSDQIKRSQ 107

Query: 96  FD 98
           +D
Sbjct: 108 YD 109

BLAST of Lsi05G015650 vs. TAIR 10
Match: AT2G35540.1 (DNAJ heat shock N-terminal domain-containing protein )

HSP 1 Score: 68.2 bits (165), Expect = 1.2e-11
Identity = 29/65 (44.62%), Postives = 46/65 (70.77%), Query Frame = 0

Query: 34  FVDWYRLFRVEQDAGIDSIRRRYLKLALQLHPDKNSHPKAEIAFKLVSEGYGCLSDNVKR 93
           F +WY++ +VE  + I++I+++Y KLAL LHPDKN +   E  FKL++E +   SD V+R
Sbjct: 69  FPEWYKVLKVEPFSHINTIKQQYRKLALVLHPDKNPYVGCEEGFKLLNEAFRVFSDKVRR 128

Query: 94  REFDL 99
            E+D+
Sbjct: 129 TEYDM 133

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9FH282.5e-1148.39Chaperone protein dnaJ 49 OS=Arabidopsis thaliana OX=3702 GN=ATJ49 PE=2 SV=2[more]
Q8TQR11.6e-1046.77Chaperone protein DnaJ OS=Methanosarcina acetivorans (strain ATCC 35395 / DSM 28... [more]
P0CW073.7e-1045.16Chaperone protein DnaJ OS=Methanosarcina mazei (strain ATCC BAA-159 / DSM 3647 /... [more]
P0CW063.7e-1045.16Chaperone protein DnaJ OS=Methanosarcina mazei OX=2209 GN=dnaJ PE=3 SV=1[more]
Q174381.1e-0946.77DnaJ homolog subfamily B member 1 OS=Caenorhabditis elegans OX=6239 GN=dnj-1 PE=... [more]
Match NameE-valueIdentityDescription
A0A5D3CXW51.7e-13085.56Chaperone protein DnaJ OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold2... [more]
A0A5A7U7H94.8e-13085.19Chaperone protein DnaJ OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold1... [more]
A0A1S3AXH76.3e-13085.24chaperone protein DnaJ OS=Cucumis melo OX=3656 GN=LOC103483872 PE=4 SV=1[more]
A0A0A0L5B21.9e-12684.50J domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G165080 PE=4 SV=1[more]
A0A6J1IXP07.2e-10269.37dnaJ homolog subfamily B member 9-like OS=Cucurbita maxima OX=3661 GN=LOC1114794... [more]
Match NameE-valueIdentityDescription
XP_038902437.11.5e-13890.71uncharacterized protein LOC120089081 [Benincasa hispida][more]
TYK16200.13.4e-13085.56chaperone protein DnaJ [Cucumis melo var. makuwa][more]
KAA0049521.19.9e-13085.19chaperone protein DnaJ [Cucumis melo var. makuwa][more]
XP_008438915.11.3e-12985.24PREDICTED: chaperone protein DnaJ [Cucumis melo][more]
XP_004134496.23.9e-12684.50uncharacterized protein LOC101213407 [Cucumis sativus] >KGN57140.1 hypothetical ... [more]
Match NameE-valueIdentityDescription
AT3G06778.13.3e-3839.91Chaperone DnaJ-domain superfamily protein [more]
AT5G18750.14.8e-1350.72DNAJ heat shock N-terminal domain-containing protein [more]
AT5G49060.11.8e-1248.39Heat shock protein DnaJ, N-terminal with domain of unknown function (DUF1977) [more]
AT3G04980.14.0e-1251.61DNAJ heat shock N-terminal domain-containing protein [more]
AT2G35540.11.2e-1144.62DNAJ heat shock N-terminal domain-containing protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 143..163
NoneNo IPR availablePANTHERPTHR44137BNAC03G44070D PROTEINcoord: 4..250
NoneNo IPR availablePANTHERPTHR44137:SF13CHAPERONE DNAJ-DOMAIN SUPERFAMILY PROTEINcoord: 4..250
IPR001623DnaJ domainPRINTSPR00625JDOMAINcoord: 38..56
score: 28.54
coord: 72..92
score: 42.4
coord: 56..71
score: 50.47
IPR001623DnaJ domainSMARTSM00271dnaj_3coord: 35..92
e-value: 2.1E-15
score: 67.2
IPR001623DnaJ domainPFAMPF00226DnaJcoord: 36..97
e-value: 5.6E-17
score: 61.6
IPR001623DnaJ domainPROSITEPS50076DNAJ_2coord: 36..100
score: 14.697661
IPR001623DnaJ domainCDDcd06257DnaJcoord: 36..89
e-value: 2.11604E-15
score: 66.8001
IPR036869Chaperone J-domain superfamilyGENE3D1.10.287.110DnaJ domaincoord: 35..151
e-value: 4.0E-19
score: 71.1
IPR036869Chaperone J-domain superfamilySUPERFAMILY46565Chaperone J-domaincoord: 25..100

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi05G015650.1Lsi05G015650.1mRNA