Lsi05G004520 (gene) Bottle gourd (USVL1VR-Ls) v1

BLAST of Lsi05G004520 vs. ExPASy Swiss-Prot
Match: Q8L1Z7 (2,3-bisphosphoglycerate-dependent phosphoglycerate mutase OS=Bartonella henselae (strain ATCC 49882 / DSM 28221 / Houston 1) OX=283166 GN=gpmA PE=3 SV=1)

HSP 1 Score: 63.5 bits (153), Expect = 7.5e-09
Identity = 42/131 (32.06%), Postives = 67/131 (51.15%), Query Frame = 0

Query: 121 LISHGECELNLRSDLVGGRCPEAGLTPKGKRQGRALAVFLKSQGVRFNAVYSSPLDRARS 180
           LI HG+ E NL++   G + P  GLT KG+ +  A    LK  G++F+  Y+S L RA+ 
Sbjct: 7   LIRHGQSEWNLKNLFTGWKDP--GLTEKGRTEAIAAGKKLKETGLKFDIAYTSALQRAQK 66

Query: 181 MAVSVCQEMNFMEEQIQNSDALTEMSQGHWERCLRSEIYTP--ETLNFIDRIQPDFSPPD 240
            A ++ ++M   + ++  + AL E + G      + E+     E    I R     +PP+
Sbjct: 67  TAQNILEQMEQSDLELIKTPALNERNYGDLSGLNKDEVRQKWGEQQVQIWRRSYTIAPPN 126

Query: 241 GESLRQVEFRM 250
           GESLR    R+
Sbjct: 127 GESLRDTGARV 135

BLAST of Lsi05G004520 vs. ExPASy Swiss-Prot
Match: P07953 (6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 1 OS=Rattus norvegicus OX=10116 GN=Pfkfb1 PE=1 SV=3)

HSP 1 Score: 57.4 bits (137), Expect = 5.4e-07
Identity = 47/156 (30.13%), Postives = 75/156 (48.08%), Query Frame = 0

Query: 119 VFLISHGECELNLRSDLVGGRCPEAGLTPKGKRQGRALAVFLKSQGVRFNAVYSSPLDRA 178
           ++L  HGE ELNLR  + G    ++GL+ +GK+   ALA F++SQG+    V++S + R 
Sbjct: 254 IYLCRHGESELNLRGRIGG----DSGLSARGKQYAYALANFIRSQGISSLKVWTSHMKR- 313

Query: 179 RSMAVSVCQEMNFMEEQIQNSDALTEMSQGHWERCLRSEI--YTPETLNFIDRIQPDFSP 238
               +   + +    EQ +   AL E+  G  E     EI  + PE     D+ +  +  
Sbjct: 314 ---TIQTAEALGVPYEQWK---ALNEIDAGVCEEMTYEEIQEHYPEEFALRDQDKYRYRY 373

Query: 239 PDGESLRQVEFRMVHFLNGTILELPEKLRSDVLSHR 273
           P GES       +V  L   I+EL  +    V+ H+
Sbjct: 374 PKGESYED----LVQRLEPVIMELERQENVLVICHQ 394

BLAST of Lsi05G004520 vs. ExPASy Swiss-Prot
Match: P16118 (6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 1 OS=Homo sapiens OX=9606 GN=PFKFB1 PE=1 SV=3)

HSP 1 Score: 55.8 bits (133), Expect = 1.6e-06
Identity = 46/156 (29.49%), Postives = 75/156 (48.08%), Query Frame = 0

Query: 119 VFLISHGECELNLRSDLVGGRCPEAGLTPKGKRQGRALAVFLKSQGVRFNAVYSSPLDRA 178
           ++L  HGE ELN+R  + G    ++GL+ +GK+   ALA F++SQG+    V++S + R 
Sbjct: 254 IYLCRHGESELNIRGRIGG----DSGLSVRGKQYAYALANFIQSQGISSLKVWTSHMKR- 313

Query: 179 RSMAVSVCQEMNFMEEQIQNSDALTEMSQGHWERCLRSEI--YTPETLNFIDRIQPDFSP 238
               +   + +    EQ +   AL E+  G  E     EI  + PE     D+ +  +  
Sbjct: 314 ---TIQTAEALGVPYEQWK---ALNEIDAGVCEEMTYEEIQEHYPEEFALRDQDKYRYRY 373

Query: 239 PDGESLRQVEFRMVHFLNGTILELPEKLRSDVLSHR 273
           P GES       +V  L   I+EL  +    V+ H+
Sbjct: 374 PKGESYED----LVQRLEPVIMELERQENVLVICHQ 394

BLAST of Lsi05G004520 vs. ExPASy Swiss-Prot
Match: P49872 (6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 1 OS=Bos taurus OX=9913 GN=PFKFB1 PE=2 SV=1)

HSP 1 Score: 55.5 bits (132), Expect = 2.0e-06
Identity = 47/156 (30.13%), Postives = 74/156 (47.44%), Query Frame = 0

Query: 119 VFLISHGECELNLRSDLVGGRCPEAGLTPKGKRQGRALAVFLKSQGVRFNAVYSSPLDRA 178
           ++L  HGE ELNLR  + G    ++GL+ +GK+   ALA F++SQG+    V +S + R 
Sbjct: 254 IYLCRHGESELNLRGRIGG----DSGLSARGKQYAYALANFIQSQGISSLKVGTSHMKR- 313

Query: 179 RSMAVSVCQEMNFMEEQIQNSDALTEMSQGHWERCLRSEI--YTPETLNFIDRIQPDFSP 238
               +   + +    EQ +   AL E+  G  E     EI  + PE     D+ +  +  
Sbjct: 314 ---TIQTAEALGLPYEQWK---ALNEIDAGVCEEMTYEEIQEHYPEEFALRDQDKYRYRY 373

Query: 239 PDGESLRQVEFRMVHFLNGTILELPEKLRSDVLSHR 273
           P GES       +V  L   I+EL  +    V+ H+
Sbjct: 374 PKGESYED----LVQRLEPVIMELERQENVLVICHQ 394

BLAST of Lsi05G004520 vs. ExPASy Swiss-Prot
Match: P70266 (6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 1 OS=Mus musculus OX=10090 GN=Pfkfb1 PE=1 SV=2)

HSP 1 Score: 55.1 bits (131), Expect = 2.7e-06
Identity = 46/156 (29.49%), Postives = 74/156 (47.44%), Query Frame = 0

Query: 119 VFLISHGECELNLRSDLVGGRCPEAGLTPKGKRQGRALAVFLKSQGVRFNAVYSSPLDRA 178
           ++L  HGE ELNLR  + G    ++GL+ +GK+   ALA F++SQ +    V++S + R 
Sbjct: 254 IYLCRHGESELNLRGRIGG----DSGLSARGKQYAYALANFIRSQSISSLKVWTSHMKR- 313

Query: 179 RSMAVSVCQEMNFMEEQIQNSDALTEMSQGHWERCLRSEI--YTPETLNFIDRIQPDFSP 238
               +   + +    EQ +   AL E+  G  E     EI  + PE     D+ +  +  
Sbjct: 314 ---TIQTAEALGVPYEQWK---ALNEIDAGVCEEMTYEEIQEHYPEEFALRDQDKYRYRY 373

Query: 239 PDGESLRQVEFRMVHFLNGTILELPEKLRSDVLSHR 273
           P GES       +V  L   I+EL  +    V+ H+
Sbjct: 374 PKGESYED----LVQRLEPVIMELERQENVLVICHQ 394

BLAST of Lsi05G004520 vs. ExPASy TrEMBL
Match: A0A1S3BNZ5 (uncharacterized protein LOC103491727 OS=Cucumis melo OX=3656 GN=LOC103491727 PE=4 SV=1)

HSP 1 Score: 715.7 bits (1846), Expect = 1.3e-202
Identity = 372/417 (89.21%), Postives = 385/417 (92.33%), Query Frame = 0

Query: 1   MGSAQSVQLAEEREEDEEEEEE-DDEDDGDDNVEPARAELDNYLVKKVLEQEPEMLPCHA 60
           MGSA SVQLAEEREE+EEEEEE DDEDDGDDNVEPARAEL NYLVKKV+EQEPEMLPCHA
Sbjct: 1   MGSAHSVQLAEEREEEEEEEEEDDDEDDGDDNVEPARAELGNYLVKKVIEQEPEMLPCHA 60

Query: 61  LASPLSPQPSSLGTPRLGPAIKVWDPYNVLAPPPQLPPPPLFTRSFSSSSIDDDRVVMEV 120
           LASPLSPQPSS+GTPRLGPAIKVWDPYNVLAPPPQLPPPP+FTRSFSSSSIDDDRVVMEV
Sbjct: 61  LASPLSPQPSSIGTPRLGPAIKVWDPYNVLAPPPQLPPPPMFTRSFSSSSIDDDRVVMEV 120

Query: 121 FLISHGECELNLRSDLVGGRCPEAGLTPKGKRQGRALAVFLKSQGVRFNAVYSSPLDRAR 180
           FLISHGECELNLRSDLVGGRCPEAGLTPKGKRQGRALAVFLKSQGVRFNAVYSSPLDRAR
Sbjct: 121 FLISHGECELNLRSDLVGGRCPEAGLTPKGKRQGRALAVFLKSQGVRFNAVYSSPLDRAR 180

Query: 181 SMAVSVCQEMNFMEEQIQNSDALTEMSQGHWERCLRSEIYTPETLNFIDRIQPDFSPPDG 240
           SMAVSVCQEMNFMEEQIQNSDALTEMSQGHWERCLRSEIYTPETLNFIDRIQPDFSPP G
Sbjct: 181 SMAVSVCQEMNFMEEQIQNSDALTEMSQGHWERCLRSEIYTPETLNFIDRIQPDFSPPAG 240

Query: 241 ESLRQVEFRMVHFLNGTILELPEKLRSDVLSHRPNETLQMSHELTNSIHDRDGHSLPSPH 300
           ESLRQVEFRMV FLNGTILELPEKLRSDVL HRPN+TLQMSHELTNSI DRDGHSLPSPH
Sbjct: 241 ESLRQVEFRMVQFLNGTILELPEKLRSDVLLHRPNDTLQMSHELTNSIRDRDGHSLPSPH 300

Query: 301 WDLPHRQRHGITRRKSGKSRLQTVTTTGEHEAEDEVSPHDATHQSTLNDLNIRNSSSCTT 360
           WDLPHRQR GITRRKSGKSRLQTVT+TGEH+ E+EVSPHDATHQSTLNDL+IRNSSSCTT
Sbjct: 301 WDLPHRQRQGITRRKSGKSRLQTVTSTGEHDVEEEVSPHDATHQSTLNDLSIRNSSSCTT 360

Query: 361 CIGIFTHSIPIKCLLAGLLECSLKDLSASMATAAVCFCVALSLATLFALSLATGSSI 417
           CIGIFTH+IPIKCLL GLLECSL  L          FC+  S  T+   SL +G  I
Sbjct: 361 CIGIFTHAIPIKCLLTGLLECSLVMLHK--------FCIEDSSVTVLQHSLKSGWQI 409

BLAST of Lsi05G004520 vs. ExPASy TrEMBL
Match: A0A6J1J699 (uncharacterized protein LOC111482146 OS=Cucurbita maxima OX=3661 GN=LOC111482146 PE=4 SV=1)

HSP 1 Score: 683.3 bits (1762), Expect = 7.4e-193
Identity = 358/422 (84.83%), Postives = 375/422 (88.86%), Query Frame = 0

Query: 1   MGSAQSVQLAEEREEDEEEEEE------DDEDDGDDNVEPARAELDNYLVKKVLEQEPEM 60
           MGSA+SVQ+AEEREEDEEEEEE      DD DDGDDNVEP R EL+NYLVKKVLEQEPEM
Sbjct: 1   MGSAESVQVAEEREEDEEEEEEEEEEEDDDGDDGDDNVEPVRVELENYLVKKVLEQEPEM 60

Query: 61  LPCHALASPLSPQPSSLGTPRLGPAIKVWDPYNVLAPPPQLPPPPLFTRSFSSSSIDDDR 120
           LPCHALASPLSPQ SSLGTPRLGPAIKVWDPYNVLAPPPQLPPPP+FTRSFSSSSIDDDR
Sbjct: 61  LPCHALASPLSPQLSSLGTPRLGPAIKVWDPYNVLAPPPQLPPPPMFTRSFSSSSIDDDR 120

Query: 121 VVMEVFLISHGECELNLRSDLVGGRCPEAGLTPKGKRQGRALAVFLKSQGVRFNAVYSSP 180
           VVMEVFLISHGECELNLRSDLVGGRCPEA LTPKGKRQ RALAVFLKSQGVRFNAV+SSP
Sbjct: 121 VVMEVFLISHGECELNLRSDLVGGRCPEAALTPKGKRQARALAVFLKSQGVRFNAVFSSP 180

Query: 181 LDRARSMAVSVCQEMNFMEEQIQNSDALTEMSQGHWERCLRSEIYTPETLNFIDRIQPDF 240
           LDRARSMAVSVCQE+NF EEQIQ SDALTEMSQGHWERCLRSEIYTPETL+FIDR+QPDF
Sbjct: 181 LDRARSMAVSVCQEINFTEEQIQTSDALTEMSQGHWERCLRSEIYTPETLSFIDRMQPDF 240

Query: 241 SPPDGESLRQVEFRMVHFLNGTILELPEKLRSDVLSHRPNETLQMSHELTNSIHDRDGHS 300
           SPP GESLRQVEFRMVHFLNGTILELPEKLRSDV+SH PNETLQ+SHELTNSIHDRD  S
Sbjct: 241 SPPAGESLRQVEFRMVHFLNGTILELPEKLRSDVVSHHPNETLQISHELTNSIHDRDSQS 300

Query: 301 LPSPHWDLPHRQRHGITRRKSGKSRLQTVTTTGEHEAEDEVSPHDATHQSTLNDLNIRNS 360
           LPS  WDLP RQRHGITR+KSGKSRLQTVTTTGEHEA+DE+SP DATHQSTLNDL IRNS
Sbjct: 301 LPSSQWDLPQRQRHGITRKKSGKSRLQTVTTTGEHEADDELSPRDATHQSTLNDLRIRNS 360

Query: 361 SSCTTCIGIFTHSIPIKCLLAGLLECSLKDLSASMATAAVCFCVALSLATLFALSLATGS 417
           SSCTTCI +FTHSIPIKCLL GLLECSL  L          FC+  S  T+   SL +G 
Sbjct: 361 SSCTTCIAVFTHSIPIKCLLTGLLECSLVMLHK--------FCIEDSSVTVLQHSLKSGW 414

BLAST of Lsi05G004520 vs. ExPASy TrEMBL
Match: A0A6J1F368 (uncharacterized protein LOC111441774 OS=Cucurbita moschata OX=3662 GN=LOC111441774 PE=4 SV=1)

HSP 1 Score: 679.9 bits (1753), Expect = 8.1e-192
Identity = 356/425 (83.76%), Postives = 375/425 (88.24%), Query Frame = 0

Query: 1   MGSAQSVQLAEEREEDEEEEEE---------DDEDDGDDNVEPARAELDNYLVKKVLEQE 60
           MGSA+SVQ+AEEREEDEEEEEE         DD DDGDDNVEP RAEL+NYLVKKVLEQE
Sbjct: 1   MGSAESVQVAEEREEDEEEEEEEEEEEEEEDDDGDDGDDNVEPVRAELENYLVKKVLEQE 60

Query: 61  PEMLPCHALASPLSPQPSSLGTPRLGPAIKVWDPYNVLAPPPQLPPPPLFTRSFSSSSID 120
           PEMLPCHALASPLSPQ SSLGTPRLGPAIKVWDPYNVLAPPPQLPPPP+FTRSFSSSSID
Sbjct: 61  PEMLPCHALASPLSPQLSSLGTPRLGPAIKVWDPYNVLAPPPQLPPPPMFTRSFSSSSID 120

Query: 121 DDRVVMEVFLISHGECELNLRSDLVGGRCPEAGLTPKGKRQGRALAVFLKSQGVRFNAVY 180
           DDRVVMEVFLISHGECELNLRSDLVGGRCPEA LTPKGKRQ RALAVFLKSQGVRFNAV+
Sbjct: 121 DDRVVMEVFLISHGECELNLRSDLVGGRCPEAALTPKGKRQARALAVFLKSQGVRFNAVF 180

Query: 181 SSPLDRARSMAVSVCQEMNFMEEQIQNSDALTEMSQGHWERCLRSEIYTPETLNFIDRIQ 240
           SSPLDRARSMA+SVCQE+NF EEQIQ SDALTEMSQGHWERCLRSEIYTPETL+FIDR+Q
Sbjct: 181 SSPLDRARSMAISVCQEINFTEEQIQTSDALTEMSQGHWERCLRSEIYTPETLSFIDRMQ 240

Query: 241 PDFSPPDGESLRQVEFRMVHFLNGTILELPEKLRSDVLSHRPNETLQMSHELTNSIHDRD 300
           PDFSPP GESLRQVEFRMVHFLNGTILELPEKLRSDV+SH PNETLQ+SHELTNSIH+RD
Sbjct: 241 PDFSPPAGESLRQVEFRMVHFLNGTILELPEKLRSDVVSHHPNETLQISHELTNSIHNRD 300

Query: 301 GHSLPSPHWDLPHRQRHGITRRKSGKSRLQTVTTTGEHEAEDEVSPHDATHQSTLNDLNI 360
             SLPS  WDLP RQRHGITR+KSGKSRLQTVTTTGEHE +DE+SP DATHQSTLNDL I
Sbjct: 301 SQSLPSSQWDLPQRQRHGITRKKSGKSRLQTVTTTGEHEVDDELSPRDATHQSTLNDLRI 360

Query: 361 RNSSSCTTCIGIFTHSIPIKCLLAGLLECSLKDLSASMATAAVCFCVALSLATLFALSLA 417
           RNSSSCTTCI +FTHSIPIKCLL GLLECSL  L          FC+  S  T+   SL 
Sbjct: 361 RNSSSCTTCIAVFTHSIPIKCLLTGLLECSLVMLHK--------FCIEDSSVTVLQHSLK 417

BLAST of Lsi05G004520 vs. ExPASy TrEMBL
Match: A0A5A7VFQ2 (Histidine phosphatase superfamily, clade-1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold595G00030 PE=4 SV=1)

HSP 1 Score: 675.6 bits (1742), Expect = 1.5e-190
Identity = 342/384 (89.06%), Postives = 354/384 (92.19%), Query Frame = 0

Query: 33  EPARAELDNYLVKKVLEQEPEMLPCHALASPLSPQPSSLGTPRLGPAIKVWDPYNVLAPP 92
           +PARAEL NYLVKKV+EQEPEMLPCHALASPLSPQPSS+GTPRLGPAIKVWDPYNVLAPP
Sbjct: 13  KPARAELGNYLVKKVIEQEPEMLPCHALASPLSPQPSSIGTPRLGPAIKVWDPYNVLAPP 72

Query: 93  PQLPPPPLFTRSFSSSSIDDDRVVMEVFLISHGECELNLRSDLVGGRCPEAGLTPKGKRQ 152
           PQLPPPP+FTRSFSSSSIDDDRVVMEVFLISHGECELNLRSDLVGGRCPEAGLTPKGKRQ
Sbjct: 73  PQLPPPPMFTRSFSSSSIDDDRVVMEVFLISHGECELNLRSDLVGGRCPEAGLTPKGKRQ 132

Query: 153 GRALAVFLKSQGVRFNAVYSSPLDRARSMAVSVCQEMNFMEEQIQNSDALTEMSQGHWER 212
           GRALAVFLKSQGVRFNAVYSSPLDRARSMAVSVCQEMNFMEEQIQNSDALTEMSQGHWER
Sbjct: 133 GRALAVFLKSQGVRFNAVYSSPLDRARSMAVSVCQEMNFMEEQIQNSDALTEMSQGHWER 192

Query: 213 CLRSEIYTPETLNFIDRIQPDFSPPDGESLRQVEFRMVHFLNGTILELPEKLRSDVLSHR 272
           CLRSEIYTPETLNFIDRIQPDFSPP GESLRQVEFRMV FLNGTILELPEKLRSDVL HR
Sbjct: 193 CLRSEIYTPETLNFIDRIQPDFSPPAGESLRQVEFRMVQFLNGTILELPEKLRSDVLLHR 252

Query: 273 PNETLQMSHELTNSIHDRDGHSLPSPHWDLPHRQRHGITRRKSGKSRLQTVTTTGEHEAE 332
           PN+TLQMSHELTNSI DRDGHSLPSPHWDLPHRQR GITRRKSGKSRLQTVT+TGEH+ E
Sbjct: 253 PNDTLQMSHELTNSIRDRDGHSLPSPHWDLPHRQRQGITRRKSGKSRLQTVTSTGEHDVE 312

Query: 333 DEVSPHDATHQSTLNDLNIRNSSSCTTCIGIFTHSIPIKCLLAGLLECSLKDLSASMATA 392
           DEVSPHDATHQSTLNDL+IRNSSSCTTCIGIFTH+IPIKCLL GLLECSL  L       
Sbjct: 313 DEVSPHDATHQSTLNDLSIRNSSSCTTCIGIFTHAIPIKCLLTGLLECSLVMLHK----- 372

Query: 393 AVCFCVALSLATLFALSLATGSSI 417
              FC+  S  T+   SL +G  I
Sbjct: 373 ---FCIEDSSVTVLQHSLKSGWQI 388

BLAST of Lsi05G004520 vs. ExPASy TrEMBL
Match: A0A6J1JQY8 (uncharacterized protein LOC111486673 OS=Cucurbita maxima OX=3661 GN=LOC111486673 PE=4 SV=1)

HSP 1 Score: 627.9 bits (1618), Expect = 3.7e-176
Identity = 338/417 (81.06%), Postives = 355/417 (85.13%), Query Frame = 0

Query: 1   MGSAQSVQLAEEREEDEEEEEEDDEDDGDDNVEPARAELDNYLVKKVLEQEPEMLPCHAL 60
           MGSAQS Q+AEEREE+EE      +DDGDDNVEPA  EL+NYLVKKVLEQEPEMLPCHAL
Sbjct: 1   MGSAQSGQVAEEREEEEE------DDDGDDNVEPASGELENYLVKKVLEQEPEMLPCHAL 60

Query: 61  ASPLSPQPSSLGTPRLGPAIKVWDPYNVLAPPPQLPPPPLFTRSFSSSSIDDDRVVMEVF 120
           ASPLSPQ SSLGTPRLGP IKVWDPYNVLAPPPQLPPPP+FTRSFSSSSIDDDRVVMEVF
Sbjct: 61  ASPLSPQLSSLGTPRLGPVIKVWDPYNVLAPPPQLPPPPVFTRSFSSSSIDDDRVVMEVF 120

Query: 121 LISHGECELNLRSDLVGGRCPEAGLTPKGKRQGRALAVFLKSQGVRFNAVYSSPLDRARS 180
           LISHGE ELNLRSDLVGGRCPEA LTPKGKRQ RALAVFLKSQGVRFNAV+SSPLDRARS
Sbjct: 121 LISHGEYELNLRSDLVGGRCPEAALTPKGKRQARALAVFLKSQGVRFNAVFSSPLDRARS 180

Query: 181 MAVSVCQEMNFMEEQIQNSDALTEMSQGHWERCLRSEIYTPETLNFIDRIQPDFSPPDGE 240
           MA SVCQEMNF EEQIQ +DALTEMS GHWE CLRS+IYTPETL+FIDRIQPDF+ P GE
Sbjct: 181 MAASVCQEMNFSEEQIQKADALTEMSHGHWEHCLRSDIYTPETLSFIDRIQPDFAAPAGE 240

Query: 241 SLRQVEFRMVHFLNGTILELPEKLRSDVLSHRPNETLQMSHELTNSIHDRDGHSL-PSPH 300
           SLRQVEFRMVHFLN TILELPEKLRSD+ + RPNETLQMSHELTNSI DRD HSL  SPH
Sbjct: 241 SLRQVEFRMVHFLNATILELPEKLRSDI-AIRPNETLQMSHELTNSILDRDAHSLQSSPH 300

Query: 301 WDLPHRQRHGITRRKSGKSRLQTVTTTGEHEAEDEVSPHDATHQSTLNDLNIRNSSSCTT 360
           WDLPHRQRHGI R+KSGKSRLQTVTTT EHEAEDE+SP DA        LNIRNSSSCTT
Sbjct: 301 WDLPHRQRHGIVRKKSGKSRLQTVTTTEEHEAEDEISPRDA-------PLNIRNSSSCTT 360

Query: 361 CIGIFTHSIPIKCLLAGLLECSLKDLSASMATAAVCFCVALSLATLFALSLATGSSI 417
           CIGIFTHSIPIKCLL GLLECSL  L          FC+  S  T+   SL +G  I
Sbjct: 361 CIGIFTHSIPIKCLLTGLLECSLAMLHK--------FCIDDSSVTVLQHSLKSGWQI 395

BLAST of Lsi05G004520 vs. NCBI nr
Match: XP_038896091.1 (uncharacterized protein LOC120084344 isoform X2 [Benincasa hispida])

HSP 1 Score: 742.7 bits (1916), Expect = 2.1e-210
Identity = 387/417 (92.81%), Postives = 392/417 (94.00%), Query Frame = 0

Query: 1   MGSAQSVQLAEEREEDEEEEEEDD-EDDGDDNVEPARAELDNYLVKKVLEQEPEMLPCHA 60
           MGSAQSVQLAEEREEDEEEEEEDD EDDGDDNVEPARAELDNYLVKKVLEQEPEMLPCHA
Sbjct: 1   MGSAQSVQLAEEREEDEEEEEEDDNEDDGDDNVEPARAELDNYLVKKVLEQEPEMLPCHA 60

Query: 61  LASPLSPQPSSLGTPRLGPAIKVWDPYNVLAPPPQLPPPPLFTRSFSSSSIDDDRVVMEV 120
           LASPLSPQPSSLGTPRLGPAIKVWDPYNVLAPPPQLPPPPLFTRSFSSSSIDDDRVVMEV
Sbjct: 61  LASPLSPQPSSLGTPRLGPAIKVWDPYNVLAPPPQLPPPPLFTRSFSSSSIDDDRVVMEV 120

Query: 121 FLISHGECELNLRSDLVGGRCPEAGLTPKGKRQGRALAVFLKSQGVRFNAVYSSPLDRAR 180
           FLISHGECELNLRSDLVGGRCPEAGLTPKGKRQGRALAVFLKSQGVRFNAVYSSPLDRAR
Sbjct: 121 FLISHGECELNLRSDLVGGRCPEAGLTPKGKRQGRALAVFLKSQGVRFNAVYSSPLDRAR 180

Query: 181 SMAVSVCQEMNFMEEQIQNSDALTEMSQGHWERCLRSEIYTPETLNFIDRIQPDFSPPDG 240
           SMAVSVCQEMNFMEEQIQNSDALTEMSQGHWERCLRSEIYTPETLNFIDRIQPDFSPP G
Sbjct: 181 SMAVSVCQEMNFMEEQIQNSDALTEMSQGHWERCLRSEIYTPETLNFIDRIQPDFSPPAG 240

Query: 241 ESLRQVEFRMVHFLNGTILELPEKLRSDVLSHRPNETLQMSHELTNSIHDRDGHSLPSPH 300
           ESLRQVEFRMVHFLNGTILELPEKLRSDVLSHRPNETLQMSHELTNSIHDRDGHSLPSPH
Sbjct: 241 ESLRQVEFRMVHFLNGTILELPEKLRSDVLSHRPNETLQMSHELTNSIHDRDGHSLPSPH 300

Query: 301 WDLPHRQRHGITRRKSGKSRLQTVTTTGEHEAEDEVSPHDATHQSTLNDLNIRNSSSCTT 360
           WDLPHRQRHGITRRKSGKSRLQTVTTTGEHEAEDE+SPHDATHQSTLNDL+IRNSS CTT
Sbjct: 301 WDLPHRQRHGITRRKSGKSRLQTVTTTGEHEAEDEISPHDATHQSTLNDLSIRNSSLCTT 360

Query: 361 CIGIFTHSIPIKCLLAGLLECSLKDLSASMATAAVCFCVALSLATLFALSLATGSSI 417
           CIGIFTHSIPIKCLLAGLLECSL  L          FC+  S  T+   SL +G  I
Sbjct: 361 CIGIFTHSIPIKCLLAGLLECSLVMLHK--------FCIEDSSVTVLQHSLKSGWQI 409

BLAST of Lsi05G004520 vs. NCBI nr
Match: XP_038896090.1 (uncharacterized protein LOC120084344 isoform X1 [Benincasa hispida])

HSP 1 Score: 735.3 bits (1897), Expect = 3.4e-208
Identity = 387/425 (91.06%), Postives = 392/425 (92.24%), Query Frame = 0

Query: 1   MGSAQSVQLAEEREEDEEEEEEDD-EDDGDDNVEPARAELDNYLVKKVLEQEPEMLPCHA 60
           MGSAQSVQLAEEREEDEEEEEEDD EDDGDDNVEPARAELDNYLVKKVLEQEPEMLPCHA
Sbjct: 1   MGSAQSVQLAEEREEDEEEEEEDDNEDDGDDNVEPARAELDNYLVKKVLEQEPEMLPCHA 60

Query: 61  LASPLSPQPSSLGTPRLGPAIKVWDPYNVLAPPPQLPPPPLFTRSFSSSSIDDDRVVMEV 120
           LASPLSPQPSSLGTPRLGPAIKVWDPYNVLAPPPQLPPPPLFTRSFSSSSIDDDRVVMEV
Sbjct: 61  LASPLSPQPSSLGTPRLGPAIKVWDPYNVLAPPPQLPPPPLFTRSFSSSSIDDDRVVMEV 120

Query: 121 FLISHGECELNLRSDLVGGRCPEAGLTPKGKRQGRALAVFLKSQGVRFNAVYSSPLDRAR 180
           FLISHGECELNLRSDLVGGRCPEAGLTPKGKRQGRALAVFLKSQGVRFNAVYSSPLDRAR
Sbjct: 121 FLISHGECELNLRSDLVGGRCPEAGLTPKGKRQGRALAVFLKSQGVRFNAVYSSPLDRAR 180

Query: 181 SMAVSVC--------QEMNFMEEQIQNSDALTEMSQGHWERCLRSEIYTPETLNFIDRIQ 240
           SMAVSVC        QEMNFMEEQIQNSDALTEMSQGHWERCLRSEIYTPETLNFIDRIQ
Sbjct: 181 SMAVSVCQTIYEMNLQEMNFMEEQIQNSDALTEMSQGHWERCLRSEIYTPETLNFIDRIQ 240

Query: 241 PDFSPPDGESLRQVEFRMVHFLNGTILELPEKLRSDVLSHRPNETLQMSHELTNSIHDRD 300
           PDFSPP GESLRQVEFRMVHFLNGTILELPEKLRSDVLSHRPNETLQMSHELTNSIHDRD
Sbjct: 241 PDFSPPAGESLRQVEFRMVHFLNGTILELPEKLRSDVLSHRPNETLQMSHELTNSIHDRD 300

Query: 301 GHSLPSPHWDLPHRQRHGITRRKSGKSRLQTVTTTGEHEAEDEVSPHDATHQSTLNDLNI 360
           GHSLPSPHWDLPHRQRHGITRRKSGKSRLQTVTTTGEHEAEDE+SPHDATHQSTLNDL+I
Sbjct: 301 GHSLPSPHWDLPHRQRHGITRRKSGKSRLQTVTTTGEHEAEDEISPHDATHQSTLNDLSI 360

Query: 361 RNSSSCTTCIGIFTHSIPIKCLLAGLLECSLKDLSASMATAAVCFCVALSLATLFALSLA 417
           RNSS CTTCIGIFTHSIPIKCLLAGLLECSL  L          FC+  S  T+   SL 
Sbjct: 361 RNSSLCTTCIGIFTHSIPIKCLLAGLLECSLVMLHK--------FCIEDSSVTVLQHSLK 417

BLAST of Lsi05G004520 vs. NCBI nr
Match: XP_008450011.1 (PREDICTED: uncharacterized protein LOC103491727 [Cucumis melo])

HSP 1 Score: 715.7 bits (1846), Expect = 2.8e-202
Identity = 372/417 (89.21%), Postives = 385/417 (92.33%), Query Frame = 0

Query: 1   MGSAQSVQLAEEREEDEEEEEE-DDEDDGDDNVEPARAELDNYLVKKVLEQEPEMLPCHA 60
           MGSA SVQLAEEREE+EEEEEE DDEDDGDDNVEPARAEL NYLVKKV+EQEPEMLPCHA
Sbjct: 1   MGSAHSVQLAEEREEEEEEEEEDDDEDDGDDNVEPARAELGNYLVKKVIEQEPEMLPCHA 60

Query: 61  LASPLSPQPSSLGTPRLGPAIKVWDPYNVLAPPPQLPPPPLFTRSFSSSSIDDDRVVMEV 120
           LASPLSPQPSS+GTPRLGPAIKVWDPYNVLAPPPQLPPPP+FTRSFSSSSIDDDRVVMEV
Sbjct: 61  LASPLSPQPSSIGTPRLGPAIKVWDPYNVLAPPPQLPPPPMFTRSFSSSSIDDDRVVMEV 120

Query: 121 FLISHGECELNLRSDLVGGRCPEAGLTPKGKRQGRALAVFLKSQGVRFNAVYSSPLDRAR 180
           FLISHGECELNLRSDLVGGRCPEAGLTPKGKRQGRALAVFLKSQGVRFNAVYSSPLDRAR
Sbjct: 121 FLISHGECELNLRSDLVGGRCPEAGLTPKGKRQGRALAVFLKSQGVRFNAVYSSPLDRAR 180

Query: 181 SMAVSVCQEMNFMEEQIQNSDALTEMSQGHWERCLRSEIYTPETLNFIDRIQPDFSPPDG 240
           SMAVSVCQEMNFMEEQIQNSDALTEMSQGHWERCLRSEIYTPETLNFIDRIQPDFSPP G
Sbjct: 181 SMAVSVCQEMNFMEEQIQNSDALTEMSQGHWERCLRSEIYTPETLNFIDRIQPDFSPPAG 240

Query: 241 ESLRQVEFRMVHFLNGTILELPEKLRSDVLSHRPNETLQMSHELTNSIHDRDGHSLPSPH 300
           ESLRQVEFRMV FLNGTILELPEKLRSDVL HRPN+TLQMSHELTNSI DRDGHSLPSPH
Sbjct: 241 ESLRQVEFRMVQFLNGTILELPEKLRSDVLLHRPNDTLQMSHELTNSIRDRDGHSLPSPH 300

Query: 301 WDLPHRQRHGITRRKSGKSRLQTVTTTGEHEAEDEVSPHDATHQSTLNDLNIRNSSSCTT 360
           WDLPHRQR GITRRKSGKSRLQTVT+TGEH+ E+EVSPHDATHQSTLNDL+IRNSSSCTT
Sbjct: 301 WDLPHRQRQGITRRKSGKSRLQTVTSTGEHDVEEEVSPHDATHQSTLNDLSIRNSSSCTT 360

Query: 361 CIGIFTHSIPIKCLLAGLLECSLKDLSASMATAAVCFCVALSLATLFALSLATGSSI 417
           CIGIFTH+IPIKCLL GLLECSL  L          FC+  S  T+   SL +G  I
Sbjct: 361 CIGIFTHAIPIKCLLTGLLECSLVMLHK--------FCIEDSSVTVLQHSLKSGWQI 409

BLAST of Lsi05G004520 vs. NCBI nr
Match: KAG7017865.1 (hypothetical protein SDJN02_19731, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 683.7 bits (1763), Expect = 1.2e-192
Identity = 359/425 (84.47%), Postives = 376/425 (88.47%), Query Frame = 0

Query: 1   MGSAQSVQLAEEREEDEEEEEE---------DDEDDGDDNVEPARAELDNYLVKKVLEQE 60
           MGSA+SVQ+AEEREEDEEEEEE         DD DDGDDNVEP RAEL+NYLVKKVLEQE
Sbjct: 1   MGSAESVQVAEEREEDEEEEEEEEEEEEEEDDDGDDGDDNVEPVRAELENYLVKKVLEQE 60

Query: 61  PEMLPCHALASPLSPQPSSLGTPRLGPAIKVWDPYNVLAPPPQLPPPPLFTRSFSSSSID 120
           PEMLPCHALASPLSPQ SSLGTPRLGPAIKVWDPYNVLAPPPQLPPPP+FTRSFSSSSID
Sbjct: 61  PEMLPCHALASPLSPQLSSLGTPRLGPAIKVWDPYNVLAPPPQLPPPPMFTRSFSSSSID 120

Query: 121 DDRVVMEVFLISHGECELNLRSDLVGGRCPEAGLTPKGKRQGRALAVFLKSQGVRFNAVY 180
           DDRVVMEVFLISHGECELNLRSDLVGGRCPEA LTPKGKRQ RALAVFLKSQGVRFNAV+
Sbjct: 121 DDRVVMEVFLISHGECELNLRSDLVGGRCPEAALTPKGKRQARALAVFLKSQGVRFNAVF 180

Query: 181 SSPLDRARSMAVSVCQEMNFMEEQIQNSDALTEMSQGHWERCLRSEIYTPETLNFIDRIQ 240
           SSPLDRARSMAVSVCQE+NF EEQIQ SDALTEMSQGHWERCLRSEIYTPETL+FIDR+Q
Sbjct: 181 SSPLDRARSMAVSVCQEINFTEEQIQTSDALTEMSQGHWERCLRSEIYTPETLSFIDRMQ 240

Query: 241 PDFSPPDGESLRQVEFRMVHFLNGTILELPEKLRSDVLSHRPNETLQMSHELTNSIHDRD 300
           PDFSPP GESLRQVEFRMVHFLNGTILELPEKLRSDV+SH PNETLQ+SHELTNSIHDRD
Sbjct: 241 PDFSPPAGESLRQVEFRMVHFLNGTILELPEKLRSDVVSHHPNETLQISHELTNSIHDRD 300

Query: 301 GHSLPSPHWDLPHRQRHGITRRKSGKSRLQTVTTTGEHEAEDEVSPHDATHQSTLNDLNI 360
             SLPS  WDLP RQRHGITR+KSGKSRLQTVTTTGEHEA+DE+SP DATHQSTLNDL I
Sbjct: 301 SQSLPSSQWDLPQRQRHGITRKKSGKSRLQTVTTTGEHEADDELSPRDATHQSTLNDLRI 360

Query: 361 RNSSSCTTCIGIFTHSIPIKCLLAGLLECSLKDLSASMATAAVCFCVALSLATLFALSLA 417
           RNSSSCTTCI +FTHSIPIKCLL GLLECSL  L          FC+  S  T+   SL 
Sbjct: 361 RNSSSCTTCIAVFTHSIPIKCLLTGLLECSLVMLHK--------FCIEDSSVTVLQHSLK 417

BLAST of Lsi05G004520 vs. NCBI nr
Match: XP_022983585.1 (uncharacterized protein LOC111482146 [Cucurbita maxima])

HSP 1 Score: 683.3 bits (1762), Expect = 1.5e-192
Identity = 358/422 (84.83%), Postives = 375/422 (88.86%), Query Frame = 0

Query: 1   MGSAQSVQLAEEREEDEEEEEE------DDEDDGDDNVEPARAELDNYLVKKVLEQEPEM 60
           MGSA+SVQ+AEEREEDEEEEEE      DD DDGDDNVEP R EL+NYLVKKVLEQEPEM
Sbjct: 1   MGSAESVQVAEEREEDEEEEEEEEEEEDDDGDDGDDNVEPVRVELENYLVKKVLEQEPEM 60

Query: 61  LPCHALASPLSPQPSSLGTPRLGPAIKVWDPYNVLAPPPQLPPPPLFTRSFSSSSIDDDR 120
           LPCHALASPLSPQ SSLGTPRLGPAIKVWDPYNVLAPPPQLPPPP+FTRSFSSSSIDDDR
Sbjct: 61  LPCHALASPLSPQLSSLGTPRLGPAIKVWDPYNVLAPPPQLPPPPMFTRSFSSSSIDDDR 120

Query: 121 VVMEVFLISHGECELNLRSDLVGGRCPEAGLTPKGKRQGRALAVFLKSQGVRFNAVYSSP 180
           VVMEVFLISHGECELNLRSDLVGGRCPEA LTPKGKRQ RALAVFLKSQGVRFNAV+SSP
Sbjct: 121 VVMEVFLISHGECELNLRSDLVGGRCPEAALTPKGKRQARALAVFLKSQGVRFNAVFSSP 180

Query: 181 LDRARSMAVSVCQEMNFMEEQIQNSDALTEMSQGHWERCLRSEIYTPETLNFIDRIQPDF 240
           LDRARSMAVSVCQE+NF EEQIQ SDALTEMSQGHWERCLRSEIYTPETL+FIDR+QPDF
Sbjct: 181 LDRARSMAVSVCQEINFTEEQIQTSDALTEMSQGHWERCLRSEIYTPETLSFIDRMQPDF 240

Query: 241 SPPDGESLRQVEFRMVHFLNGTILELPEKLRSDVLSHRPNETLQMSHELTNSIHDRDGHS 300
           SPP GESLRQVEFRMVHFLNGTILELPEKLRSDV+SH PNETLQ+SHELTNSIHDRD  S
Sbjct: 241 SPPAGESLRQVEFRMVHFLNGTILELPEKLRSDVVSHHPNETLQISHELTNSIHDRDSQS 300

Query: 301 LPSPHWDLPHRQRHGITRRKSGKSRLQTVTTTGEHEAEDEVSPHDATHQSTLNDLNIRNS 360
           LPS  WDLP RQRHGITR+KSGKSRLQTVTTTGEHEA+DE+SP DATHQSTLNDL IRNS
Sbjct: 301 LPSSQWDLPQRQRHGITRKKSGKSRLQTVTTTGEHEADDELSPRDATHQSTLNDLRIRNS 360

Query: 361 SSCTTCIGIFTHSIPIKCLLAGLLECSLKDLSASMATAAVCFCVALSLATLFALSLATGS 417
           SSCTTCI +FTHSIPIKCLL GLLECSL  L          FC+  S  T+   SL +G 
Sbjct: 361 SSCTTCIAVFTHSIPIKCLLTGLLECSLVMLHK--------FCIEDSSVTVLQHSLKSGW 414

BLAST of Lsi05G004520 vs. TAIR 10
Match: AT3G26780.1 (Phosphoglycerate mutase family protein )

HSP 1 Score: 419.9 bits (1078), Expect = 2.9e-117
Identity = 238/385 (61.82%), Postives = 286/385 (74.29%), Query Frame = 0

Query: 1   MGSAQSVQLAEEREEDEE--EEEEDDEDDGDDNVEPARAELDNYLVKKVLEQEPEMLPCH 60
           MGS+QS Q+ +E EE+EE   EEE++ED+G  N      +LDN LVKKVLEQEPEMLPCH
Sbjct: 1   MGSSQSTQMEDEEEEEEETDNEEEEEEDNGRSNTR----QLDNLLVKKVLEQEPEMLPCH 60

Query: 61  ALASPLSPQPSSLGTPRLGPAIKVWDPYNVLAPPPQLPPPPLFTRSFSSSSIDDDRVVME 120
           A ASPLSPQ SSLGTPRLGP+IKVWDPYNVL+PPPQ  PP +F+R  S    D+DR V E
Sbjct: 61  ASASPLSPQLSSLGTPRLGPSIKVWDPYNVLSPPPQ--PPSIFSRIVSG---DEDRAVTE 120

Query: 121 VFLISHGECELNLRSDLVGGRCPEAGLTPKGKRQGRALAVFLKSQGVRFNAVYSSPLDRA 180
           V+LISHGE +LNL+ DL+GGRC  A LT  GKRQ RALAVF KSQGVRFN+VYSSPLDRA
Sbjct: 121 VYLISHGESDLNLKPDLIGGRCHVAALTANGKRQARALAVFFKSQGVRFNSVYSSPLDRA 180

Query: 181 RSMAVSVCQEMNFMEEQIQNSDALTEMSQGHWERCLRSEIYTPETLNFIDRIQPDFSPPD 240
           RSMAVSVCQEM+F EE +Q+SDA+ EMS G WE C ++EIY+PETL+ I+R QPDF+ P 
Sbjct: 181 RSMAVSVCQEMSFPEEHVQSSDAVIEMSLGDWEGCNQAEIYSPETLSLIERCQPDFTAPS 240

Query: 241 GESLRQVEFRMVHFLNGTILELPEKLRSDVLSHRPNETLQMSHELTNSIHDRDGH-SLPS 300
           GESLRQVEFRMV FLNGT+  + EKLRSD  S         +H   N  H+RDG  SL S
Sbjct: 241 GESLRQVEFRMVQFLNGTVSGVAEKLRSDYSS-------TTNH---NETHERDGGVSLAS 300

Query: 301 PHWDLPHRQRHGITRRKSGKSRLQTVTTTGEHEAEDEVSPHDATHQSTLNDLNIRNSSS- 360
            +WDL H+ R  +TR+KSGKSRLQ +T    HE +D  SP +  +    N  ++ +SSS 
Sbjct: 301 TNWDLLHKHRPSLTRKKSGKSRLQVMT---NHEPDDGNSPREEVNH---NHTDLSDSSSL 360

Query: 361 CTTCIGIFTHSIPIKCLLAGLLECS 382
            + C+G+FTHS+PIKCLL G+L CS
Sbjct: 361 ISNCVGVFTHSLPIKCLLTGILGCS 360

BLAST of Lsi05G004520 vs. TAIR 10
Match: AT1G12850.1 (Phosphoglycerate mutase family protein )

HSP 1 Score: 416.4 bits (1069), Expect = 3.2e-116
Identity = 237/388 (61.08%), Postives = 278/388 (71.65%), Query Frame = 0

Query: 1   MGSAQSVQLAEEREEDEEEEE------EDDEDDGDDNVEPARAELDNYLVKKVLEQEPEM 60
           MGS+QS QL +E EE+E+E E      E++ED+ +  +   R ELDN LVKKVLEQEPEM
Sbjct: 1   MGSSQSSQLLDEEEEEEDEAESEGEEVEEEEDEAESELNNRRIELDNLLVKKVLEQEPEM 60

Query: 61  LPCHALASPLSPQPSSLGTPRLGPAIKVWDPYNVLAPPPQLPPPPLFTRSFSSSSIDDDR 120
           LPCHA ASPLSPQ SSLGTPR+GP+IKVWDPYNVLAPPP    PPLF+R  SS++  D  
Sbjct: 61  LPCHASASPLSPQLSSLGTPRIGPSIKVWDPYNVLAPPPP-SSPPLFSR-ISSAAEHDRS 120

Query: 121 VVMEVFLISHGECELNLRSDLVGGRCPEAGLTPKGKRQGRALAVFLKSQGVRFNAVYSSP 180
            V EV+ ISHGEC+LNLR DL+GGRC  A LTP GKRQ RALAVFL S+GVRF +V+SSP
Sbjct: 121 AVTEVYFISHGECDLNLRPDLIGGRCHVATLTPNGKRQARALAVFLNSEGVRFTSVFSSP 180

Query: 181 LDRARSMAVSVCQEMNFMEEQIQNSDALTEMSQGHWERCLRSEIYTPETLNFIDRIQPDF 240
           LDRARSMAVSVCQEMNF EE +Q SDA+ EMS G WE C RSEIYTPETL+ I+R QPDF
Sbjct: 181 LDRARSMAVSVCQEMNFPEEHLQVSDAVVEMSLGDWESCHRSEIYTPETLSLIERCQPDF 240

Query: 241 SPPDGESLRQVEFRMVHFLNGTILELPEKLRSDVLSHRPNETLQMSHELTNSIHDRDGHS 300
           S P GESLRQVEFRMV FLNGT+  L EKLRS++L      T      L  SIH      
Sbjct: 241 SAPSGESLRQVEFRMVQFLNGTVSGLSEKLRSELLP-STQHTNSRGFSLATSIH------ 300

Query: 301 LPSPHWDLPHRQRHGITRRKSGKSRLQTVTTTGEHEAEDEVSPHDATHQSTLNDLNIRNS 360
                       R  +TR+KSGKSR Q +  TG+HE  +E+  +    Q  L D+NI++S
Sbjct: 301 ------------RPILTRKKSGKSRFQVMNATGDHEGSEEIFSNHNDEQH-LGDINIKSS 360

Query: 361 SS-CTTCIGIFTHSIPIKCLLAGLLECS 382
           SS  +TCIG+FTHS+PIKCLL G+L CS
Sbjct: 361 SSQLSTCIGVFTHSLPIKCLLTGILGCS 366