Lsi05G003830 (gene) Bottle gourd (USVL1VR-Ls) v1

BLAST of Lsi05G003830 vs. ExPASy Swiss-Prot
Match: Q9FF18 (Cytokinin hydroxylase OS=Arabidopsis thaliana OX=3702 GN=CYP735A1 PE=1 SV=1)

HSP 1 Score: 707.2 bits (1824), Expect = 1.3e-202
Identity = 348/518 (67.18%), Postives = 419/518 (80.89%), Query Frame = 0

Query: 3   IVIFLTTLLVIFISSLAKIAYDTISCYWLTPRRIRKKMEKQGVRGPEPRPLLGNILDVAS 62
           ++  L +LLVIF++++ ++ YDTISCYWLTPRRI+K ME+QGV GP+PRPL GNIL++++
Sbjct: 2   LLTILKSLLVIFVTTILRVLYDTISCYWLTPRRIKKIMEQQGVTGPKPRPLTGNILEISA 61

Query: 63  LVAKSTANDMNSIDHDIVDRLLPHYTTWTKQYGKRFVYWNGTEPRLCLAETELIKEVLSK 122
           +V++S + D +SI HDIV RLLPHY  W+KQYGKRF+ WNGT+PRLCL ETELIKE+L K
Sbjct: 62  MVSQSASKDCDSIHHDIVGRLLPHYVAWSKQYGKRFIVWNGTDPRLCLTETELIKELLMK 121

Query: 123 NSNVCGKSWLQRQGTKHFIGKGLLMANGEDC------------------YAGMMVECTKK 182
           ++ V G+SWLQ+QGTK+FIG+GLLMANG+D                   YA  MVECT K
Sbjct: 122 HNGVSGRSWLQQQGTKNFIGRGLLMANGQDWHHQRHLAAPAFTGERLKGYARHMVECTSK 181

Query: 183 MIQSLETELDSGRTEFEIADYMKQLTADIISRTEFDANSDKGKEIFHLLTLLQRLCAQAS 242
           +++ L  E+  G  E EI + M +LTADIISRT+F ++ +KGKE+F+ LT+LQR CAQA+
Sbjct: 182 LVERLRKEVGEGANEVEIGEEMHKLTADIISRTKFGSSFEKGKELFNHLTVLQRRCAQAT 241

Query: 243 RHLCLPGSRFFPSKYNREIKSLKMEVERLLMEIIQSRKDGVEIGRSTCYGSDLLGMLLNE 302
           RHLC PGSRF PSKYNREIKSLK EVERLL+EIIQSR+D  E+GRS+ +G DLLG+LLNE
Sbjct: 242 RHLCFPGSRFLPSKYNREIKSLKKEVERLLIEIIQSRRDCAEMGRSSTHGDDLLGLLLNE 301

Query: 303 MQRKREDNGLSLNLQLIMDECKTFFFAGHETTALLLTWTIMLLATNPNWQHKVRHEVNQV 362
           M   + +N  + NLQLIMDECKTFFFAGHETTALLLTWT MLLA NP WQ KVR EV +V
Sbjct: 302 MDIDKNNNNNNNNLQLIMDECKTFFFAGHETTALLLTWTTMLLADNPTWQEKVREEVREV 361

Query: 363 CNNNGGTPSVEHLSKLTLLNMVINESLRLYPPATVLPRMAFEDIKVGDLEIPKGLSIWIP 422
              N G PSV+ LSKLT L+ VINESLRLYPPAT+LPRMAFED+K+GDL IPKGLSIWIP
Sbjct: 362 FGRN-GLPSVDQLSKLTSLSKVINESLRLYPPATLLPRMAFEDLKLGDLTIPKGLSIWIP 421

Query: 423 VLAIHHSEELWGKDANEFKPERFASKPFSGGR-FIPFASGPRNCIGQGFALMEAKIILAM 482
           VLAIHHSEELWGKDAN+F PERF  +PF+ GR FIPFA+GPRNCIGQ FALMEAKIILA 
Sbjct: 422 VLAIHHSEELWGKDANQFNPERFGGRPFASGRHFIPFAAGPRNCIGQQFALMEAKIILAT 481

Query: 483 FLSKFSFTISDSYRHAPVSVLTIQPKYGVQVYLTPINS 502
            +SKF+FTIS +YRHAP+ VLTI+PKYGVQV L P+ S
Sbjct: 482 LISKFNFTISKNYRHAPIVVLTIKPKYGVQVILKPLVS 518

BLAST of Lsi05G003830 vs. ExPASy Swiss-Prot
Match: Q9ZW95 (Cytokinin hydroxylase OS=Arabidopsis thaliana OX=3702 GN=CYP735A2 PE=1 SV=1)

HSP 1 Score: 682.6 bits (1760), Expect = 3.5e-195
Identity = 342/519 (65.90%), Postives = 412/519 (79.38%), Query Frame = 0

Query: 1   MAIVIFLTTLLVIFISSLAKIAYDTISCYWLTPRRIRKKMEKQGVRGPEPRPLLGNILDV 60
           M + + L  +LVI ++ + ++ YD+I CY+LTPRRI+K ME+QG+ GP+PR L GNI+D+
Sbjct: 1   MMVTLVLKYVLVIVMTLILRVLYDSICCYFLTPRRIKKFMERQGITGPKPRLLTGNIIDI 60

Query: 61  ASLVAKSTANDMNSIDHDIVDRLLPHYTTWTKQYGKRFVYWNGTEPRLCLAETELIKEVL 120
           + +++ S +ND +SI H+IV RLLPHY +W+KQYGKRF+ WNGTEPRLCL ETE+IKE+L
Sbjct: 61  SKMLSHSASNDCSSIHHNIVPRLLPHYVSWSKQYGKRFIMWNGTEPRLCLTETEMIKELL 120

Query: 121 SKNSNVCGKSWLQRQGTKHFIGKGLLMANGE------------------DCYAGMMVECT 180
           +K++ V GKSWLQ+QGTK FIG+GLLMANGE                    YA  MVECT
Sbjct: 121 TKHNPVTGKSWLQQQGTKGFIGRGLLMANGEAWHHQRHMAAPAFTRDRLKGYAKHMVECT 180

Query: 181 KKMIQSLETELDSGRTEFEIADYMKQLTADIISRTEFDANSDKGKEIFHLLTLLQRLCAQ 240
           K M + L  E+     E EI + M++LTADIISRTEF ++ DKGKE+F LLT+LQRLCAQ
Sbjct: 181 KMMAERLRKEVGE---EVEIGEEMRRLTADIISRTEFGSSCDKGKELFSLLTVLQRLCAQ 240

Query: 241 ASRHLCLPGSRFFPSKYNREIKSLKMEVERLLMEIIQSRKDGVEIGRSTCYGSDLLGMLL 300
           A+RHLC PGSRF PSKYNREIKSLK EVERLLMEII SRKD VEIGRS+ YG DLLG+LL
Sbjct: 241 ATRHLCFPGSRFLPSKYNREIKSLKTEVERLLMEIIDSRKDSVEIGRSSSYGDDLLGLLL 300

Query: 301 NEMQRKREDNGLSLNLQLIMDECKTFFFAGHETTALLLTWTIMLLATNPNWQHKVRHEVN 360
           N+M    + N  +LN+Q+IMDECKTFFF GHETT+LLLTWT+MLLA NP WQ  VR EV 
Sbjct: 301 NQM----DSNKNNLNVQMIMDECKTFFFTGHETTSLLLTWTLMLLAHNPTWQDNVRDEVR 360

Query: 361 QVCNNNGGTPSVEHLSKLTLLNMVINESLRLYPPATVLPRMAFEDIKVGDLEIPKGLSIW 420
           QVC  + G PSVE LS LT LN VINESLRLYPPAT+LPRMAFEDIK+GDL IPKGLSIW
Sbjct: 361 QVCGQD-GVPSVEQLSSLTSLNKVINESLRLYPPATLLPRMAFEDIKLGDLIIPKGLSIW 420

Query: 421 IPVLAIHHSEELWGKDANEFKPERFASKPFSGGR-FIPFASGPRNCIGQGFALMEAKIIL 480
           IPVLAIHHS ELWG+DANEF PERF ++ F+  R F+PFA+GPRNCIGQ FA+MEAKIIL
Sbjct: 421 IPVLAIHHSNELWGEDANEFNPERFTTRSFASSRHFMPFAAGPRNCIGQTFAMMEAKIIL 480

Query: 481 AMFLSKFSFTISDSYRHAPVSVLTIQPKYGVQVYLTPIN 501
           AM +SKFSF IS++YRHAP+ VLTI+PKYGVQ+ L P++
Sbjct: 481 AMLVSKFSFAISENYRHAPIVVLTIKPKYGVQLVLKPLD 511

BLAST of Lsi05G003830 vs. ExPASy Swiss-Prot
Match: F4IK45 (Cytochrome P450 709B2 OS=Arabidopsis thaliana OX=3702 GN=CYP709B2 PE=2 SV=1)

HSP 1 Score: 339.0 bits (868), Expect = 9.4e-92
Identity = 191/492 (38.82%), Postives = 279/492 (56.71%), Query Frame = 0

Query: 33  PRRIRKKMEKQGVRGPEPRPLLGNILDVASLVAKSTANDMNSIDHDIVDRLLPHYTTWTK 92
           P  + ++ +KQG+ GP+ R L GN+ ++  +  ++    ++   +DIV R+LPH   W  
Sbjct: 88  PWMLSRRFKKQGISGPKYRILYGNLREIRKMKNEAKLMVLDPNSNDIVPRVLPHLQQWKS 147

Query: 93  QYGKRFVYWNGTEPRLCLAETELIKEVLSKNSNVCGKSWLQRQGTKHFIGKGLLMANGED 152
           QYG+ F+YW GT+PRLC+++ EL K++LS       KS  + +  K   G GL+  NG D
Sbjct: 148 QYGETFLYWQGTDPRLCISDHELAKQILSNKFVFFSKSKTKPEILK-LSGNGLIFVNGLD 207

Query: 153 ------------------CYAGMMVECTKKMIQSLETELDSGRTE--FEIADYMKQLTAD 212
                                 +MV+CT +M    + + +   TE    I+   K+LTAD
Sbjct: 208 WVRHRRILNPAFSMDKLKLMTQLMVDCTFRMFLEWKKQRNGVETEQFVLISREFKRLTAD 267

Query: 213 IISRTEFDANSDKGKEIFHLLTLLQRLCAQASRHLCLPGSRFFPSKYNREIKSLKMEVER 272
           II+   F ++  +G E+F     LQ+ CA A   L  PG ++ P+  N +I  L M+V  
Sbjct: 268 IIATAAFGSSYAEGIEVFKSQLELQKCCAAALTDLYFPGIQYLPTPSNLQIWKLDMKVNS 327

Query: 273 LLMEIIQSRKDGVEIGRSTCYGSDLLGMLLNEMQRKREDNGLSLNLQLIMDECKTFFFAG 332
            +  II +R        S  YG+DLLG++L        +  +S++   I++ECKTFFFAG
Sbjct: 328 SIKRIIDARL----TSESKDYGNDLLGIMLTAASSNESEKKMSID--EIIEECKTFFFAG 387

Query: 333 HETTALLLTWTIMLLATNPNWQHKVRHEVNQVCNNNGGTPSVEHLSKLTLLNMVINESLR 392
           HETTA LLTW+ MLL+ + +WQ K+R EV   C  +   P  E  SKL L+N V  ESLR
Sbjct: 388 HETTANLLTWSTMLLSLHQDWQEKLREEVFNECGKD-KIPDAETCSKLKLMNTVFMESLR 447

Query: 393 LYPPATVLPRMAFEDIKVGDLEIPKGLSIWIPVLAIHHSEELWGKDANEFKPERFASKPF 452
           LY P   L R+A ED+K+G+LEIPKG +I +P+  +H  + +WG DA++F P RFA+   
Sbjct: 448 LYGPVLNLLRLASEDMKLGNLEIPKGTTIILPIAKMHRDKAVWGSDADKFNPMRFANGLS 507

Query: 453 SGGR----FIPFASGPRNCIGQGFALMEAKIILAMFLSKFSFTISDSYRHAPVSVLTIQP 501
                    + F+ GPR CIGQ FA+MEAK +LAM L +F   +S  Y+HAP   LT+QP
Sbjct: 508 RAANHPNALLAFSMGPRACIGQNFAIMEAKTVLAMILQRFRLNLSADYKHAPADHLTLQP 567

BLAST of Lsi05G003830 vs. ExPASy Swiss-Prot
Match: Q9ASR3 (Cytochrome P450 709B1 OS=Arabidopsis thaliana OX=3702 GN=CYP709B1 PE=2 SV=1)

HSP 1 Score: 337.8 bits (865), Expect = 2.1e-91
Identity = 194/527 (36.81%), Postives = 297/527 (56.36%), Query Frame = 0

Query: 1   MAIVIFLTTLLVIFISSLAKIAYDTISCYWLTPRRIRKKMEKQGVRGPEPRPLLGNILDV 60
           M +VIFL  +++I I  L +I    +   W  P  + ++++ QG+ GP  R   GN+ ++
Sbjct: 1   MGLVIFLALIVLILIIGL-RIFKAFMILVW-HPFVLTRRLKNQGISGPNYRIFYGNLSEI 60

Query: 61  ASLVAKSTANDMNSIDHDIVDRLLPHYTTWTKQYGKRFVYWNGTEPRLCLAETELIKEVL 120
             +  +S  + ++   +DI  R+LPHY  W  QYG+ F+YWNGTEPR+C+++ EL K +L
Sbjct: 61  KKMKRESHLSILDPSSNDIFPRILPHYQKWMSQYGETFLYWNGTEPRICISDPELAKTML 120

Query: 121 SKNSNVCGKSWLQRQGTKHFIGKGLLMANGED------------------CYAGMMVECT 180
           S       KS  + +  K    KGL+   G D                      +MV+CT
Sbjct: 121 SNKLGFFVKSKARPEAVKLVGSKGLVFIEGADWVRHRRILNPAFSIDRLKIMTTVMVDCT 180

Query: 181 KKMIQSLETELDSGRTEF-----EIADYMKQLTADIISRTEFDANSDKGKEIFHLLTLLQ 240
            KM++    E     TE      E+ +  ++LTADII+ + F ++  +G E+F     L+
Sbjct: 181 LKMLEEWRKESTKEETEHPKIKKEMNEEFQRLTADIIATSAFGSSYVEGIEVFRSQMELK 240

Query: 241 RLCAQASRHLCLPGSRFFPSKYNREIKSLKMEVERLLMEIIQSRKDGVEIGRSTCYGSDL 300
           R    +   + +PG+++ P+  N  +  L+ +++  +  II SR     +   + YG DL
Sbjct: 241 RCYTTSLNQVSIPGTQYLPTPSNIRVWKLERKMDNSIKRIISSR-----LQSKSDYGDDL 300

Query: 301 LGMLLNEMQRKREDNGLSLNLQLIMDECKTFFFAGHETTALLLTWTIMLLATNPNWQHKV 360
           LG+LL     + ++  +S  ++ I+ EC+TFFF GHETT+ LL WT MLL+ + +WQ K+
Sbjct: 301 LGILLKAYNTEGKERKMS--IEEIIHECRTFFFGGHETTSNLLAWTTMLLSLHQDWQEKL 360

Query: 361 RHEVNQVCNNNGGTPSVEHLSKLTLLNMVINESLRLYPPATVLPRMAFEDIKVGDLEIPK 420
           R E+ + C     TP  E  SKL L+NMVI ESLRLY P + L R A  +IK+GDLEIPK
Sbjct: 361 REEIFKECGKE-KTPDSETFSKLKLMNMVIMESLRLYGPVSALAREASVNIKLGDLEIPK 420

Query: 421 GLSIWIPVLAIHHSEELWGKDANEFKPERFASKPFSGGR----FIPFASGPRNCIGQGFA 480
           G ++ IP+L +H  + LWG DA++F P RFA+            + F+ GPR CIGQ F 
Sbjct: 421 GTTVVIPLLKMHSDKTLWGSDADKFNPMRFANGVSRAANHPNALLAFSVGPRACIGQNFV 480

Query: 481 LMEAKIILAMFLSKFSF-TISDSYRHAPVSVLTIQPKYGVQVYLTPI 500
           ++EAK +L M L +F F ++ D Y+H PV  +TIQP+YG+ V L P+
Sbjct: 481 MIEAKTVLTMILQRFRFISLCDEYKHTPVDNVTIQPQYGLPVMLQPL 517

BLAST of Lsi05G003830 vs. ExPASy Swiss-Prot
Match: W8JWW3 (Cytochrome P450 72A225 OS=Catharanthus roseus OX=4058 GN=CYP72A225 PE=2 SV=1)

HSP 1 Score: 315.8 bits (808), Expect = 8.5e-85
Identity = 178/521 (34.17%), Postives = 298/521 (57.20%), Query Frame = 0

Query: 5   IFLTTLLVIFISSLAKIAYDTISCYWLTPRRIRKKMEKQGVRGPEPRPLLGNILDVASLV 64
           +F   + V+       +A+  ++  W+ P+++ K++ + G++G   R L+G++ + + + 
Sbjct: 20  VFYRGISVLGTCFFLLLAWKMLNWAWIKPKKLEKRLRQLGLKGNSYRLLIGDLGETSQMD 79

Query: 65  AKSTANDMNSIDHDIVDRLLPHYTTWTKQYGKRFVYWNGTEPRLCLAETELIKEVLSKN- 124
            ++ +  +N  +H I+ R++P      + +GK    W G  P + + + ELIKEVL+KN 
Sbjct: 80  KEAKSKPINFTNH-IIPRIMPFLHKIIQSHGKNPFIWQGPNPAVIITDPELIKEVLTKNY 139

Query: 125 ------SNVCGK-------SWLQRQGTKH--FIGKGLLMANGEDCYAGMMVECTK--KMI 184
                 S+  GK       ++ + +  KH   +     +   +       + C++  K  
Sbjct: 140 TYQKPPSDDLGKLISQGVAAYDKDKWAKHRRLLNPAFHIEKLKHMLPAFHLSCSEMLKKW 199

Query: 185 QSLETELDSGRTEFEIADYMKQLTADIISRTEFDANSDKGKEIFHLLTLLQRLCAQASRH 244
           + L +    G  E ++  Y++ +T+D+ISRT F +N ++GK IF L   +     Q  + 
Sbjct: 200 EKLASASAEGSFELDVWPYLQDMTSDVISRTAFGSNLEEGKNIFKLQKEILEHILQILQS 259

Query: 245 LCLPGSRFFPSKYNREIKSLKMEVERLLMEIIQSRKDGVEIGRSTCYGSDLLGMLLNEMQ 304
              PG  + P+K  R +K +  EV   ++ II  R + ++ G  +    DLLG+LL+   
Sbjct: 260 FYFPGMLYLPTKRIRRMKKISKEVHSSVLNIIMKRVEAMKAGEPS--NDDLLGILLDSNF 319

Query: 305 RKRE-DNG------LSLNLQLIMDECKTFFFAGHETTALLLTWTIMLLATNPNWQHKVRH 364
           ++ E DNG        ++ + +++ECK F+FAG ETTA+LL WT++LL+ +P WQ + R 
Sbjct: 320 KEIENDNGNNSNKNCGMSFEEVIEECKLFYFAGQETTAVLLVWTLLLLSKHPEWQMRARD 379

Query: 365 EVNQVCNNNGGTPSVEHLSKLTLLNMVINESLRLYPPATVLPRMAFEDIKVGDLEIPKGL 424
           EV Q+  N+   P  + L+ L ++ MVINE LRLYPP  ++ R+  ED K+G L +P G+
Sbjct: 380 EVLQIFGNS--EPDFDALNHLKVITMVINEVLRLYPPGFMMSRVIHEDTKLGSLSLPGGI 439

Query: 425 SIWIPVLAIHHSEELWGKDANEFKPERFAS--KPFSGGRFI--PFASGPRNCIGQGFALM 484
           SI++P + +HH EELWG DA EFKPERF+      + G+FI  PF+ GPR CIG  FA++
Sbjct: 440 SIFLPTIMLHHDEELWGDDAKEFKPERFSEGISKATKGQFIYFPFSGGPRICIGNNFAML 499

Query: 485 EAKIILAMFLSKFSFTISDSYRHAPVSVLTIQPKYGVQVYL 497
           EAK+ LA+ L +F+F IS SY HAP +++T+QP+YG Q+ L
Sbjct: 500 EAKMALALILQRFTFEISPSYTHAPHTLVTLQPQYGAQLIL 535

BLAST of Lsi05G003830 vs. ExPASy TrEMBL
Match: A0A1S3CGC0 (cytokinin hydroxylase-like OS=Cucumis melo OX=3656 GN=LOC103500156 PE=3 SV=1)

HSP 1 Score: 941.4 bits (2432), Expect = 1.5e-270
Identity = 471/521 (90.40%), Postives = 487/521 (93.47%), Query Frame = 0

Query: 1   MAIVIFLTTLLVIFISSLAKIAYDTISCYWLTPRRIRKKMEKQGVRGPEPRPLLGNILDV 60
           MAI+IFLTTLLVIFISSLAKIAYDTISCYWLTPRRIRKKMEKQGVRGP PRPL+GNILDV
Sbjct: 1   MAIIIFLTTLLVIFISSLAKIAYDTISCYWLTPRRIRKKMEKQGVRGPVPRPLVGNILDV 60

Query: 61  ASLVAKSTANDMNSIDHDIVDRLLPHYTTWTKQYGKRFVYWNGTEPRLCLAETELIKEVL 120
           A+L+AKSTA DM+SIDH+IVDRLLPHYTTWTKQYGKRF+YWNG EPRLCLAETELIKEVL
Sbjct: 61  AALLAKSTAKDMSSIDHNIVDRLLPHYTTWTKQYGKRFIYWNGMEPRLCLAETELIKEVL 120

Query: 121 SKNSNVCGKSWLQRQGTKHFIGKGLLMANGED------------------CYAGMMVECT 180
           SKNSNVCG+SWLQRQGTKHFIGKGLLMANGED                   YAG+MVECT
Sbjct: 121 SKNSNVCGRSWLQRQGTKHFIGKGLLMANGEDWYHQRHIVAPSFMGDKLKSYAGLMVECT 180

Query: 181 KKMIQSLETELDSGRTEFEIADYMKQLTADIISRTEFDANSDKGKEIFHLLTLLQRLCAQ 240
            K+IQSLETELDSGRTEFEIADYMKQLTADIISRTEFDANSDKG +IFHLLTLLQ LCAQ
Sbjct: 181 DKLIQSLETELDSGRTEFEIADYMKQLTADIISRTEFDANSDKGNQIFHLLTLLQHLCAQ 240

Query: 241 ASRHLCLPGSRFFPSKYNREIKSLKMEVERLLMEIIQSRKDGVEIGRSTCYGSDLLGMLL 300
           ASRHLCLPGSRFFPSKYNREIKSLKMEVERLLMEIIQSRKDGVEIGRSTCYGSDLLGMLL
Sbjct: 241 ASRHLCLPGSRFFPSKYNREIKSLKMEVERLLMEIIQSRKDGVEIGRSTCYGSDLLGMLL 300

Query: 301 NEMQRKRE--DNGLSLNLQLIMDECKTFFFAGHETTALLLTWTIMLLATNPNWQHKVRHE 360
           NEMQRK++  DNG SLNLQLIMDECKTFFFAGHETTALLLTWTIMLLATNPNWQ KVRHE
Sbjct: 301 NEMQRKKDDGDNGFSLNLQLIMDECKTFFFAGHETTALLLTWTIMLLATNPNWQQKVRHE 360

Query: 361 VNQVCNNNGGTPSVEHLSKLTLLNMVINESLRLYPPATVLPRMAFEDIKVGDLEIPKGLS 420
           VNQVC +NG TPSVEHLSK TLLNMVINESLRLYPPATVLPRMAFEDIKVGDLEIPKGLS
Sbjct: 361 VNQVC-HNGATPSVEHLSKFTLLNMVINESLRLYPPATVLPRMAFEDIKVGDLEIPKGLS 420

Query: 421 IWIPVLAIHHSEELWGKDANEFKPERFASKPFSGGRFIPFASGPRNCIGQGFALMEAKII 480
           IWIPVLAIHHSEELWGKD NEFKPERFASKPFSGGRFIPFA+GPRNC+GQGFALMEAKII
Sbjct: 421 IWIPVLAIHHSEELWGKDVNEFKPERFASKPFSGGRFIPFAAGPRNCVGQGFALMEAKII 480

Query: 481 LAMFLSKFSFTISDSYRHAPVSVLTIQPKYGVQVYLTPINS 502
           LAM LSKFSFTISDSYRHAPVSVLTIQPKYGVQVYLTPINS
Sbjct: 481 LAMLLSKFSFTISDSYRHAPVSVLTIQPKYGVQVYLTPINS 520

BLAST of Lsi05G003830 vs. ExPASy TrEMBL
Match: A0A0A0L6Y6 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G271920 PE=3 SV=1)

HSP 1 Score: 932.9 bits (2410), Expect = 5.4e-268
Identity = 467/521 (89.64%), Postives = 483/521 (92.71%), Query Frame = 0

Query: 1   MAIVIFLTTLLVIFISSLAKIAYDTISCYWLTPRRIRKKMEKQGVRGPEPRPLLGNILDV 60
           MAI+I LTTLLVIFISSLAKIAYDTISCYWLTPRRIRKKMEKQGVRGP PRPL+GNILDV
Sbjct: 1   MAIMIILTTLLVIFISSLAKIAYDTISCYWLTPRRIRKKMEKQGVRGPVPRPLVGNILDV 60

Query: 61  ASLVAKSTANDMNSIDHDIVDRLLPHYTTWTKQYGKRFVYWNGTEPRLCLAETELIKEVL 120
           A+L+ KSTA DM+SIDH+IVDRLLPHYTTWTKQYGKRF+YWNG EPRLCLAETELIKEVL
Sbjct: 61  AALLGKSTAKDMSSIDHNIVDRLLPHYTTWTKQYGKRFIYWNGMEPRLCLAETELIKEVL 120

Query: 121 SKNSNVCGKSWLQRQGTKHFIGKGLLMANGED------------------CYAGMMVECT 180
           SKNSNVCG+SWLQRQGTKHFIGKGLLMANGED                   YAG+MVECT
Sbjct: 121 SKNSNVCGRSWLQRQGTKHFIGKGLLMANGEDWYHQRHIVAPSFMGDKLKSYAGLMVECT 180

Query: 181 KKMIQSLETELDSGRTEFEIADYMKQLTADIISRTEFDANSDKGKEIFHLLTLLQRLCAQ 240
            K+IQSLETE DSGRTEFEIADYMKQLTADIISRTEFD NSDKG +IFHLLTLLQ LCAQ
Sbjct: 181 NKLIQSLETEFDSGRTEFEIADYMKQLTADIISRTEFDCNSDKGNQIFHLLTLLQHLCAQ 240

Query: 241 ASRHLCLPGSRFFPSKYNREIKSLKMEVERLLMEIIQSRKDGVEIGRSTCYGSDLLGMLL 300
           ASRHLCLPGSRFFPSKYNREIKSLKMEVERLLMEIIQSRKDGVEIGRSTCYGSDLLGMLL
Sbjct: 241 ASRHLCLPGSRFFPSKYNREIKSLKMEVERLLMEIIQSRKDGVEIGRSTCYGSDLLGMLL 300

Query: 301 NEMQRKRE--DNGLSLNLQLIMDECKTFFFAGHETTALLLTWTIMLLATNPNWQHKVRHE 360
           NEMQ++RE  DNG SLNLQLIMDECKTFFFAGHETTALLLTWTIMLLATNPNWQ KVRHE
Sbjct: 301 NEMQKRREDGDNGFSLNLQLIMDECKTFFFAGHETTALLLTWTIMLLATNPNWQQKVRHE 360

Query: 361 VNQVCNNNGGTPSVEHLSKLTLLNMVINESLRLYPPATVLPRMAFEDIKVGDLEIPKGLS 420
           VNQVC +NG TPSVEHLSK TLLNMVINESLRLYPPATVLPRMAFEDIKVGDLEIPKGLS
Sbjct: 361 VNQVC-HNGATPSVEHLSKFTLLNMVINESLRLYPPATVLPRMAFEDIKVGDLEIPKGLS 420

Query: 421 IWIPVLAIHHSEELWGKDANEFKPERFASKPFSGGRFIPFASGPRNCIGQGFALMEAKII 480
           IWIPVLAIHHSEELWGKD NEFKPERFASKPFSGGRFIPFA+GPRNCIGQGFALMEAKII
Sbjct: 421 IWIPVLAIHHSEELWGKDVNEFKPERFASKPFSGGRFIPFAAGPRNCIGQGFALMEAKII 480

Query: 481 LAMFLSKFSFTISDSYRHAPVSVLTIQPKYGVQVYLTPINS 502
           LAM LSKFSF+ISDSYRHAPVSVLTIQPKYGVQVYLTPINS
Sbjct: 481 LAMLLSKFSFSISDSYRHAPVSVLTIQPKYGVQVYLTPINS 520

BLAST of Lsi05G003830 vs. ExPASy TrEMBL
Match: A0A5A7V8T6 (Cytokinin hydroxylase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold508G00410 PE=3 SV=1)

HSP 1 Score: 873.2 bits (2255), Expect = 5.1e-250
Identity = 433/482 (89.83%), Postives = 448/482 (92.95%), Query Frame = 0

Query: 40  MEKQGVRGPEPRPLLGNILDVASLVAKSTANDMNSIDHDIVDRLLPHYTTWTKQYGKRFV 99
           MEKQGVRGP PRPL+GNILDVA+L+AKSTA DM+SIDH+IVDRLLPHYTTWTKQYGKRF+
Sbjct: 1   MEKQGVRGPVPRPLVGNILDVAALLAKSTAKDMSSIDHNIVDRLLPHYTTWTKQYGKRFI 60

Query: 100 YWNGTEPRLCLAETELIKEVLSKNSNVCGKSWLQRQGTKHFIGKGLLMANGED------- 159
           YWNG EPRLCLAETELIKEVLSKNSNVCG+SWLQRQGTKHFIGKGLLMANGED       
Sbjct: 61  YWNGMEPRLCLAETELIKEVLSKNSNVCGRSWLQRQGTKHFIGKGLLMANGEDWYHQRHI 120

Query: 160 -----------CYAGMMVECTKKMIQSLETELDSGRTEFEIADYMKQLTADIISRTEFDA 219
                       YAG+MVECT K+IQSLETELDSGRTEFEIADYMKQLTADIISRTEFDA
Sbjct: 121 VAPSFMGDKLKSYAGLMVECTDKLIQSLETELDSGRTEFEIADYMKQLTADIISRTEFDA 180

Query: 220 NSDKGKEIFHLLTLLQRLCAQASRHLCLPGSRFFPSKYNREIKSLKMEVERLLMEIIQSR 279
           NSDKG +IFHLLTLLQ LCAQASRHLCLPGSRFFPSKYNREIKSLKMEVERLLMEIIQSR
Sbjct: 181 NSDKGNQIFHLLTLLQHLCAQASRHLCLPGSRFFPSKYNREIKSLKMEVERLLMEIIQSR 240

Query: 280 KDGVEIGRSTCYGSDLLGMLLNEMQRKRE--DNGLSLNLQLIMDECKTFFFAGHETTALL 339
           KDGVEIGRSTCYGSDLLGMLLNEMQRK++  DNG SLNLQLIMDECKTFFFAGHETTALL
Sbjct: 241 KDGVEIGRSTCYGSDLLGMLLNEMQRKKDDGDNGFSLNLQLIMDECKTFFFAGHETTALL 300

Query: 340 LTWTIMLLATNPNWQHKVRHEVNQVCNNNGGTPSVEHLSKLTLLNMVINESLRLYPPATV 399
           LTWTIMLLATNPNWQ KVRHEVNQVC +NG TPSVEHLSK TLLNMVINESLRLYPPATV
Sbjct: 301 LTWTIMLLATNPNWQQKVRHEVNQVC-HNGATPSVEHLSKFTLLNMVINESLRLYPPATV 360

Query: 400 LPRMAFEDIKVGDLEIPKGLSIWIPVLAIHHSEELWGKDANEFKPERFASKPFSGGRFIP 459
           LPRMAFEDIKVGDLEIPKGLSIWIPVLAIHHSEELWGKD NEFKPERFASKPFSGGRFIP
Sbjct: 361 LPRMAFEDIKVGDLEIPKGLSIWIPVLAIHHSEELWGKDVNEFKPERFASKPFSGGRFIP 420

Query: 460 FASGPRNCIGQGFALMEAKIILAMFLSKFSFTISDSYRHAPVSVLTIQPKYGVQVYLTPI 502
           FA+GPRNC+GQGFALMEAKIILAM LSKFSFTISDSYRHAPVSVLTIQPKYGVQVYLTPI
Sbjct: 421 FAAGPRNCVGQGFALMEAKIILAMLLSKFSFTISDSYRHAPVSVLTIQPKYGVQVYLTPI 480

BLAST of Lsi05G003830 vs. ExPASy TrEMBL
Match: A0A6J1J2Y1 (cytokinin hydroxylase-like OS=Cucurbita maxima OX=3661 GN=LOC111482203 PE=3 SV=1)

HSP 1 Score: 864.8 bits (2233), Expect = 1.8e-247
Identity = 430/519 (82.85%), Postives = 462/519 (89.02%), Query Frame = 0

Query: 1   MAIVIFLTTLLVIFISSLAKIAYDTISCYWLTPRRIRKKMEKQGVRGPEPRPLLGNILDV 60
           MA +IFLTTL VIFISSLAKI+YDT S Y LTPRRIRK ME+QGVRGP+PR L+GNILDV
Sbjct: 1   MAFIIFLTTLFVIFISSLAKISYDTFSFYCLTPRRIRKTMEQQGVRGPKPRLLIGNILDV 60

Query: 61  ASLVAKSTANDMNSIDHDIVDRLLPHYTTWTKQYGKRFVYWNGTEPRLCLAETELIKEVL 120
             LVAKSTANDM+SIDHDIVDRLLPHYT WTKQYGKRF+YWNGTEPRLCLAETELIKE+L
Sbjct: 61  VGLVAKSTANDMSSIDHDIVDRLLPHYTIWTKQYGKRFIYWNGTEPRLCLAETELIKELL 120

Query: 121 SKNSNVCGKSWLQRQGTKHFIGKGLLMANGED------------------CYAGMMVECT 180
            K S+VCG+SWLQRQGTKHFIGKGLLMANGED                   YAG MVECT
Sbjct: 121 GKKSSVCGRSWLQRQGTKHFIGKGLLMANGEDWYHQRHIVAPAFMGDKLKSYAGFMVECT 180

Query: 181 KKMIQSLETELDSGRTEFEIADYMKQLTADIISRTEFDANSDKGKEIFHLLTLLQRLCAQ 240
            KMIQS+ETELDSGRTEFEIADYMKQLTADIISRTEFD +SDKG +IFHLLTLLQRLCAQ
Sbjct: 181 NKMIQSMETELDSGRTEFEIADYMKQLTADIISRTEFDTDSDKGNQIFHLLTLLQRLCAQ 240

Query: 241 ASRHLCLPGSRFFPSKYNREIKSLKMEVERLLMEIIQSRKDGVEIGRSTCYGSDLLGMLL 300
           ASRHLCLPGSRF PSKYNREIKSLK EVERLLMEII+SRKDGVEIGRS+CYGSDLLGMLL
Sbjct: 241 ASRHLCLPGSRFVPSKYNREIKSLKKEVERLLMEIIESRKDGVEIGRSSCYGSDLLGMLL 300

Query: 301 NEMQRKREDNGLSLNLQLIMDECKTFFFAGHETTALLLTWTIMLLATNPNWQHKVRHEVN 360
           NEMQ+++     +LNLQLI+DECKTFFFAGHETTALLLTWTIMLLATNPNWQH VR EVN
Sbjct: 301 NEMQQRKNSTTTTLNLQLIIDECKTFFFAGHETTALLLTWTIMLLATNPNWQHMVRAEVN 360

Query: 361 QVCNNNGGTPSVEHLSKLTLLNMVINESLRLYPPATVLPRMAFEDIKVGDLEIPKGLSIW 420
           QVC  N  TPS++HLSKLTLLNMVINESLRLYPPATVLPRMA ED+K+GD++IPKGLSIW
Sbjct: 361 QVC--NARTPSIQHLSKLTLLNMVINESLRLYPPATVLPRMALEDMKLGDMDIPKGLSIW 420

Query: 421 IPVLAIHHSEELWGKDANEFKPERFASKPFSGGRFIPFASGPRNCIGQGFALMEAKIILA 480
           IPVLAIHHSEELWGKDAN+F P RFA+K F GGRFIPFA GPRNCIGQGFA+MEAKIILA
Sbjct: 421 IPVLAIHHSEELWGKDANQFNPNRFAAKAFGGGRFIPFAGGPRNCIGQGFAIMEAKIILA 480

Query: 481 MFLSKFSFTISDSYRHAPVSVLTIQPKYGVQVYLTPINS 502
           MFLSKFSFTISD+YRHAPV VLTIQPK+GVQV LTP +S
Sbjct: 481 MFLSKFSFTISDTYRHAPVVVLTIQPKHGVQVCLTPFSS 517

BLAST of Lsi05G003830 vs. ExPASy TrEMBL
Match: A0A6J1F8S1 (cytokinin hydroxylase OS=Cucurbita moschata OX=3662 GN=LOC111441866 PE=3 SV=1)

HSP 1 Score: 852.0 bits (2200), Expect = 1.2e-243
Identity = 425/519 (81.89%), Postives = 460/519 (88.63%), Query Frame = 0

Query: 1   MAIVIFLTTLLVIFISSLAKIAYDTISCYWLTPRRIRKKMEKQGVRGPEPRPLLGNILDV 60
           MA +IFLTTLL+IF++SLA+I+YDT S Y LTPRRIRK ME+QGVRGP+PR L+GNILDV
Sbjct: 1   MAFIIFLTTLLLIFLASLARISYDTFSFYCLTPRRIRKTMEQQGVRGPKPRLLIGNILDV 60

Query: 61  ASLVAKSTANDMNSIDHDIVDRLLPHYTTWTKQYGKRFVYWNGTEPRLCLAETELIKEVL 120
           A LVAKSTAN M+SIDHDIVDRLLPHYT WTKQYGKRF+YWNGTEPRLCLAETELIKE+L
Sbjct: 61  AGLVAKSTANHMSSIDHDIVDRLLPHYTIWTKQYGKRFIYWNGTEPRLCLAETELIKELL 120

Query: 121 SKNSNVCGKSWLQRQGTKHFIGKGLLMANGED------------------CYAGMMVECT 180
            K S+VCG+SWLQRQGTKHFIGKGLLMANGED                   YAG MVECT
Sbjct: 121 GKKSSVCGRSWLQRQGTKHFIGKGLLMANGEDWYHQRHIMAPAFMGDKLKSYAGFMVECT 180

Query: 181 KKMIQSLETELDSGRTEFEIADYMKQLTADIISRTEFDANSDKGKEIFHLLTLLQRLCAQ 240
            KMIQS+ETEL+SGRTEFEIADYMKQLTADIISRTEFD +SDKG +IFHLLTLLQRLCAQ
Sbjct: 181 NKMIQSMETELESGRTEFEIADYMKQLTADIISRTEFDTDSDKGNQIFHLLTLLQRLCAQ 240

Query: 241 ASRHLCLPGSRFFPSKYNREIKSLKMEVERLLMEIIQSRKDGVEIGRSTCYGSDLLGMLL 300
           ASRHLCLPGSRF PSKYNREIKSLK EVERLLMEII+SRKDGVEIGRS+CYGSDLLGMLL
Sbjct: 241 ASRHLCLPGSRFVPSKYNREIKSLKKEVERLLMEIIESRKDGVEIGRSSCYGSDLLGMLL 300

Query: 301 NEMQRKREDNGLSLNLQLIMDECKTFFFAGHETTALLLTWTIMLLATNPNWQHKVRHEVN 360
           NEMQ +R++   +LNLQLIMDECKTFFFAGHETTALLLTWTIMLLATNPNWQH VR +VN
Sbjct: 301 NEMQ-QRKNISTTLNLQLIMDECKTFFFAGHETTALLLTWTIMLLATNPNWQHMVRAQVN 360

Query: 361 QVCNNNGGTPSVEHLSKLTLLNMVINESLRLYPPATVLPRMAFEDIKVGDLEIPKGLSIW 420
           QVC  N   PS++HLSKLTLLNMVINESLRLYPPATVLPRMA ED+K+GD++IPKGLSIW
Sbjct: 361 QVC--NARAPSIQHLSKLTLLNMVINESLRLYPPATVLPRMALEDMKLGDMDIPKGLSIW 420

Query: 421 IPVLAIHHSEELWGKDANEFKPERFASKPFSGGRFIPFASGPRNCIGQGFALMEAKIILA 480
           IPVLAIHHSEELWGKDAN+F P RF +K F GGRFIPFA GPRNCIGQGFA+MEAKIILA
Sbjct: 421 IPVLAIHHSEELWGKDANQFNPNRFGAKAFGGGRFIPFAGGPRNCIGQGFAIMEAKIILA 480

Query: 481 MFLSKFSFTISDSYRHAPVSVLTIQPKYGVQVYLTPINS 502
           MFLSKF FTISD+YRHAPV VLTIQPKYGVQV LTP  S
Sbjct: 481 MFLSKFRFTISDTYRHAPVVVLTIQPKYGVQVCLTPFTS 516

BLAST of Lsi05G003830 vs. NCBI nr
Match: XP_038905687.1 (cytokinin hydroxylase-like [Benincasa hispida])

HSP 1 Score: 967.2 bits (2499), Expect = 5.4e-278
Identity = 484/519 (93.26%), Postives = 494/519 (95.18%), Query Frame = 0

Query: 1   MAIVIFLTTLLVIFISSLAKIAYDTISCYWLTPRRIRKKMEKQGVRGPEPRPLLGNILDV 60
           MAIVIFLTT+LVIFISSLAKIAYDTISCYWLTPRRIRKKMEKQGV GP+PRPLLGNILDV
Sbjct: 1   MAIVIFLTTILVIFISSLAKIAYDTISCYWLTPRRIRKKMEKQGVCGPDPRPLLGNILDV 60

Query: 61  ASLVAKSTANDMNSIDHDIVDRLLPHYTTWTKQYGKRFVYWNGTEPRLCLAETELIKEVL 120
           A+LVAKSTANDM SIDH+IV RLLPHYTTWTKQYGKRF+YWNGTEPRLCLAETELIKEVL
Sbjct: 61  AALVAKSTANDMKSIDHNIVGRLLPHYTTWTKQYGKRFIYWNGTEPRLCLAETELIKEVL 120

Query: 121 SKNSNVCGKSWLQRQGTKHFIGKGLLMANGED------------------CYAGMMVECT 180
           SKNSNVCGKSWLQRQGTKHFIGKGLLMANGED                   YAG+MVECT
Sbjct: 121 SKNSNVCGKSWLQRQGTKHFIGKGLLMANGEDWYHQRHIVAPAFMGDKLKSYAGLMVECT 180

Query: 181 KKMIQSLETELDSGRTEFEIADYMKQLTADIISRTEFDANSDKGKEIFHLLTLLQRLCAQ 240
           KKMIQSLETE DSGRTEFEIADYMKQLTADIISRTEFDANSDKGKEIFHLLTLLQRLCAQ
Sbjct: 181 KKMIQSLETEFDSGRTEFEIADYMKQLTADIISRTEFDANSDKGKEIFHLLTLLQRLCAQ 240

Query: 241 ASRHLCLPGSRFFPSKYNREIKSLKMEVERLLMEIIQSRKDGVEIGRSTCYGSDLLGMLL 300
           ASRHLCLPGSRFFPSKYNREIKSLKMEVERLLMEIIQSRKDGVEIGRSTCYGSDLLGMLL
Sbjct: 241 ASRHLCLPGSRFFPSKYNREIKSLKMEVERLLMEIIQSRKDGVEIGRSTCYGSDLLGMLL 300

Query: 301 NEMQRKREDNGLSLNLQLIMDECKTFFFAGHETTALLLTWTIMLLATNPNWQHKVRHEVN 360
           NEMQRKREDNG SLNLQLIMDECKTFFFAGHETTALLLTWTIMLLATNP+WQHKVRHEVN
Sbjct: 301 NEMQRKREDNGFSLNLQLIMDECKTFFFAGHETTALLLTWTIMLLATNPDWQHKVRHEVN 360

Query: 361 QVCNNNGGTPSVEHLSKLTLLNMVINESLRLYPPATVLPRMAFEDIKVGDLEIPKGLSIW 420
           QVC NNGG+PSVEHLSKLTLLNMVINESLRLYPPATVLPRMAFEDIKVGDLEIPKGLSIW
Sbjct: 361 QVC-NNGGSPSVEHLSKLTLLNMVINESLRLYPPATVLPRMAFEDIKVGDLEIPKGLSIW 420

Query: 421 IPVLAIHHSEELWGKDANEFKPERFASKPFSGGRFIPFASGPRNCIGQGFALMEAKIILA 480
           IPVLAIHHSEELWG+DANEFKPERFASKPFSGGRFIPFASGPRNCIGQGFALMEAKIILA
Sbjct: 421 IPVLAIHHSEELWGEDANEFKPERFASKPFSGGRFIPFASGPRNCIGQGFALMEAKIILA 480

Query: 481 MFLSKFSFTISDSYRHAPVSVLTIQPKYGVQVYLTPINS 502
           MF+SKFSFTISDSYRHAPVSVLTIQPKYGVQVYLTPINS
Sbjct: 481 MFISKFSFTISDSYRHAPVSVLTIQPKYGVQVYLTPINS 518

BLAST of Lsi05G003830 vs. NCBI nr
Match: XP_008461587.1 (PREDICTED: cytokinin hydroxylase-like [Cucumis melo])

HSP 1 Score: 941.4 bits (2432), Expect = 3.1e-270
Identity = 471/521 (90.40%), Postives = 487/521 (93.47%), Query Frame = 0

Query: 1   MAIVIFLTTLLVIFISSLAKIAYDTISCYWLTPRRIRKKMEKQGVRGPEPRPLLGNILDV 60
           MAI+IFLTTLLVIFISSLAKIAYDTISCYWLTPRRIRKKMEKQGVRGP PRPL+GNILDV
Sbjct: 1   MAIIIFLTTLLVIFISSLAKIAYDTISCYWLTPRRIRKKMEKQGVRGPVPRPLVGNILDV 60

Query: 61  ASLVAKSTANDMNSIDHDIVDRLLPHYTTWTKQYGKRFVYWNGTEPRLCLAETELIKEVL 120
           A+L+AKSTA DM+SIDH+IVDRLLPHYTTWTKQYGKRF+YWNG EPRLCLAETELIKEVL
Sbjct: 61  AALLAKSTAKDMSSIDHNIVDRLLPHYTTWTKQYGKRFIYWNGMEPRLCLAETELIKEVL 120

Query: 121 SKNSNVCGKSWLQRQGTKHFIGKGLLMANGED------------------CYAGMMVECT 180
           SKNSNVCG+SWLQRQGTKHFIGKGLLMANGED                   YAG+MVECT
Sbjct: 121 SKNSNVCGRSWLQRQGTKHFIGKGLLMANGEDWYHQRHIVAPSFMGDKLKSYAGLMVECT 180

Query: 181 KKMIQSLETELDSGRTEFEIADYMKQLTADIISRTEFDANSDKGKEIFHLLTLLQRLCAQ 240
            K+IQSLETELDSGRTEFEIADYMKQLTADIISRTEFDANSDKG +IFHLLTLLQ LCAQ
Sbjct: 181 DKLIQSLETELDSGRTEFEIADYMKQLTADIISRTEFDANSDKGNQIFHLLTLLQHLCAQ 240

Query: 241 ASRHLCLPGSRFFPSKYNREIKSLKMEVERLLMEIIQSRKDGVEIGRSTCYGSDLLGMLL 300
           ASRHLCLPGSRFFPSKYNREIKSLKMEVERLLMEIIQSRKDGVEIGRSTCYGSDLLGMLL
Sbjct: 241 ASRHLCLPGSRFFPSKYNREIKSLKMEVERLLMEIIQSRKDGVEIGRSTCYGSDLLGMLL 300

Query: 301 NEMQRKRE--DNGLSLNLQLIMDECKTFFFAGHETTALLLTWTIMLLATNPNWQHKVRHE 360
           NEMQRK++  DNG SLNLQLIMDECKTFFFAGHETTALLLTWTIMLLATNPNWQ KVRHE
Sbjct: 301 NEMQRKKDDGDNGFSLNLQLIMDECKTFFFAGHETTALLLTWTIMLLATNPNWQQKVRHE 360

Query: 361 VNQVCNNNGGTPSVEHLSKLTLLNMVINESLRLYPPATVLPRMAFEDIKVGDLEIPKGLS 420
           VNQVC +NG TPSVEHLSK TLLNMVINESLRLYPPATVLPRMAFEDIKVGDLEIPKGLS
Sbjct: 361 VNQVC-HNGATPSVEHLSKFTLLNMVINESLRLYPPATVLPRMAFEDIKVGDLEIPKGLS 420

Query: 421 IWIPVLAIHHSEELWGKDANEFKPERFASKPFSGGRFIPFASGPRNCIGQGFALMEAKII 480
           IWIPVLAIHHSEELWGKD NEFKPERFASKPFSGGRFIPFA+GPRNC+GQGFALMEAKII
Sbjct: 421 IWIPVLAIHHSEELWGKDVNEFKPERFASKPFSGGRFIPFAAGPRNCVGQGFALMEAKII 480

Query: 481 LAMFLSKFSFTISDSYRHAPVSVLTIQPKYGVQVYLTPINS 502
           LAM LSKFSFTISDSYRHAPVSVLTIQPKYGVQVYLTPINS
Sbjct: 481 LAMLLSKFSFTISDSYRHAPVSVLTIQPKYGVQVYLTPINS 520

BLAST of Lsi05G003830 vs. NCBI nr
Match: XP_004145660.1 (cytokinin hydroxylase [Cucumis sativus] >KGN57750.1 hypothetical protein Csa_009800 [Cucumis sativus])

HSP 1 Score: 932.9 bits (2410), Expect = 1.1e-267
Identity = 467/521 (89.64%), Postives = 483/521 (92.71%), Query Frame = 0

Query: 1   MAIVIFLTTLLVIFISSLAKIAYDTISCYWLTPRRIRKKMEKQGVRGPEPRPLLGNILDV 60
           MAI+I LTTLLVIFISSLAKIAYDTISCYWLTPRRIRKKMEKQGVRGP PRPL+GNILDV
Sbjct: 1   MAIMIILTTLLVIFISSLAKIAYDTISCYWLTPRRIRKKMEKQGVRGPVPRPLVGNILDV 60

Query: 61  ASLVAKSTANDMNSIDHDIVDRLLPHYTTWTKQYGKRFVYWNGTEPRLCLAETELIKEVL 120
           A+L+ KSTA DM+SIDH+IVDRLLPHYTTWTKQYGKRF+YWNG EPRLCLAETELIKEVL
Sbjct: 61  AALLGKSTAKDMSSIDHNIVDRLLPHYTTWTKQYGKRFIYWNGMEPRLCLAETELIKEVL 120

Query: 121 SKNSNVCGKSWLQRQGTKHFIGKGLLMANGED------------------CYAGMMVECT 180
           SKNSNVCG+SWLQRQGTKHFIGKGLLMANGED                   YAG+MVECT
Sbjct: 121 SKNSNVCGRSWLQRQGTKHFIGKGLLMANGEDWYHQRHIVAPSFMGDKLKSYAGLMVECT 180

Query: 181 KKMIQSLETELDSGRTEFEIADYMKQLTADIISRTEFDANSDKGKEIFHLLTLLQRLCAQ 240
            K+IQSLETE DSGRTEFEIADYMKQLTADIISRTEFD NSDKG +IFHLLTLLQ LCAQ
Sbjct: 181 NKLIQSLETEFDSGRTEFEIADYMKQLTADIISRTEFDCNSDKGNQIFHLLTLLQHLCAQ 240

Query: 241 ASRHLCLPGSRFFPSKYNREIKSLKMEVERLLMEIIQSRKDGVEIGRSTCYGSDLLGMLL 300
           ASRHLCLPGSRFFPSKYNREIKSLKMEVERLLMEIIQSRKDGVEIGRSTCYGSDLLGMLL
Sbjct: 241 ASRHLCLPGSRFFPSKYNREIKSLKMEVERLLMEIIQSRKDGVEIGRSTCYGSDLLGMLL 300

Query: 301 NEMQRKRE--DNGLSLNLQLIMDECKTFFFAGHETTALLLTWTIMLLATNPNWQHKVRHE 360
           NEMQ++RE  DNG SLNLQLIMDECKTFFFAGHETTALLLTWTIMLLATNPNWQ KVRHE
Sbjct: 301 NEMQKRREDGDNGFSLNLQLIMDECKTFFFAGHETTALLLTWTIMLLATNPNWQQKVRHE 360

Query: 361 VNQVCNNNGGTPSVEHLSKLTLLNMVINESLRLYPPATVLPRMAFEDIKVGDLEIPKGLS 420
           VNQVC +NG TPSVEHLSK TLLNMVINESLRLYPPATVLPRMAFEDIKVGDLEIPKGLS
Sbjct: 361 VNQVC-HNGATPSVEHLSKFTLLNMVINESLRLYPPATVLPRMAFEDIKVGDLEIPKGLS 420

Query: 421 IWIPVLAIHHSEELWGKDANEFKPERFASKPFSGGRFIPFASGPRNCIGQGFALMEAKII 480
           IWIPVLAIHHSEELWGKD NEFKPERFASKPFSGGRFIPFA+GPRNCIGQGFALMEAKII
Sbjct: 421 IWIPVLAIHHSEELWGKDVNEFKPERFASKPFSGGRFIPFAAGPRNCIGQGFALMEAKII 480

Query: 481 LAMFLSKFSFTISDSYRHAPVSVLTIQPKYGVQVYLTPINS 502
           LAM LSKFSF+ISDSYRHAPVSVLTIQPKYGVQVYLTPINS
Sbjct: 481 LAMLLSKFSFSISDSYRHAPVSVLTIQPKYGVQVYLTPINS 520

BLAST of Lsi05G003830 vs. NCBI nr
Match: KAA0063407.1 (cytokinin hydroxylase-like [Cucumis melo var. makuwa])

HSP 1 Score: 873.2 bits (2255), Expect = 1.1e-249
Identity = 433/482 (89.83%), Postives = 448/482 (92.95%), Query Frame = 0

Query: 40  MEKQGVRGPEPRPLLGNILDVASLVAKSTANDMNSIDHDIVDRLLPHYTTWTKQYGKRFV 99
           MEKQGVRGP PRPL+GNILDVA+L+AKSTA DM+SIDH+IVDRLLPHYTTWTKQYGKRF+
Sbjct: 1   MEKQGVRGPVPRPLVGNILDVAALLAKSTAKDMSSIDHNIVDRLLPHYTTWTKQYGKRFI 60

Query: 100 YWNGTEPRLCLAETELIKEVLSKNSNVCGKSWLQRQGTKHFIGKGLLMANGED------- 159
           YWNG EPRLCLAETELIKEVLSKNSNVCG+SWLQRQGTKHFIGKGLLMANGED       
Sbjct: 61  YWNGMEPRLCLAETELIKEVLSKNSNVCGRSWLQRQGTKHFIGKGLLMANGEDWYHQRHI 120

Query: 160 -----------CYAGMMVECTKKMIQSLETELDSGRTEFEIADYMKQLTADIISRTEFDA 219
                       YAG+MVECT K+IQSLETELDSGRTEFEIADYMKQLTADIISRTEFDA
Sbjct: 121 VAPSFMGDKLKSYAGLMVECTDKLIQSLETELDSGRTEFEIADYMKQLTADIISRTEFDA 180

Query: 220 NSDKGKEIFHLLTLLQRLCAQASRHLCLPGSRFFPSKYNREIKSLKMEVERLLMEIIQSR 279
           NSDKG +IFHLLTLLQ LCAQASRHLCLPGSRFFPSKYNREIKSLKMEVERLLMEIIQSR
Sbjct: 181 NSDKGNQIFHLLTLLQHLCAQASRHLCLPGSRFFPSKYNREIKSLKMEVERLLMEIIQSR 240

Query: 280 KDGVEIGRSTCYGSDLLGMLLNEMQRKRE--DNGLSLNLQLIMDECKTFFFAGHETTALL 339
           KDGVEIGRSTCYGSDLLGMLLNEMQRK++  DNG SLNLQLIMDECKTFFFAGHETTALL
Sbjct: 241 KDGVEIGRSTCYGSDLLGMLLNEMQRKKDDGDNGFSLNLQLIMDECKTFFFAGHETTALL 300

Query: 340 LTWTIMLLATNPNWQHKVRHEVNQVCNNNGGTPSVEHLSKLTLLNMVINESLRLYPPATV 399
           LTWTIMLLATNPNWQ KVRHEVNQVC +NG TPSVEHLSK TLLNMVINESLRLYPPATV
Sbjct: 301 LTWTIMLLATNPNWQQKVRHEVNQVC-HNGATPSVEHLSKFTLLNMVINESLRLYPPATV 360

Query: 400 LPRMAFEDIKVGDLEIPKGLSIWIPVLAIHHSEELWGKDANEFKPERFASKPFSGGRFIP 459
           LPRMAFEDIKVGDLEIPKGLSIWIPVLAIHHSEELWGKD NEFKPERFASKPFSGGRFIP
Sbjct: 361 LPRMAFEDIKVGDLEIPKGLSIWIPVLAIHHSEELWGKDVNEFKPERFASKPFSGGRFIP 420

Query: 460 FASGPRNCIGQGFALMEAKIILAMFLSKFSFTISDSYRHAPVSVLTIQPKYGVQVYLTPI 502
           FA+GPRNC+GQGFALMEAKIILAM LSKFSFTISDSYRHAPVSVLTIQPKYGVQVYLTPI
Sbjct: 421 FAAGPRNCVGQGFALMEAKIILAMLLSKFSFTISDSYRHAPVSVLTIQPKYGVQVYLTPI 480

BLAST of Lsi05G003830 vs. NCBI nr
Match: XP_022983651.1 (cytokinin hydroxylase-like [Cucurbita maxima])

HSP 1 Score: 864.8 bits (2233), Expect = 3.7e-247
Identity = 430/519 (82.85%), Postives = 462/519 (89.02%), Query Frame = 0

Query: 1   MAIVIFLTTLLVIFISSLAKIAYDTISCYWLTPRRIRKKMEKQGVRGPEPRPLLGNILDV 60
           MA +IFLTTL VIFISSLAKI+YDT S Y LTPRRIRK ME+QGVRGP+PR L+GNILDV
Sbjct: 1   MAFIIFLTTLFVIFISSLAKISYDTFSFYCLTPRRIRKTMEQQGVRGPKPRLLIGNILDV 60

Query: 61  ASLVAKSTANDMNSIDHDIVDRLLPHYTTWTKQYGKRFVYWNGTEPRLCLAETELIKEVL 120
             LVAKSTANDM+SIDHDIVDRLLPHYT WTKQYGKRF+YWNGTEPRLCLAETELIKE+L
Sbjct: 61  VGLVAKSTANDMSSIDHDIVDRLLPHYTIWTKQYGKRFIYWNGTEPRLCLAETELIKELL 120

Query: 121 SKNSNVCGKSWLQRQGTKHFIGKGLLMANGED------------------CYAGMMVECT 180
            K S+VCG+SWLQRQGTKHFIGKGLLMANGED                   YAG MVECT
Sbjct: 121 GKKSSVCGRSWLQRQGTKHFIGKGLLMANGEDWYHQRHIVAPAFMGDKLKSYAGFMVECT 180

Query: 181 KKMIQSLETELDSGRTEFEIADYMKQLTADIISRTEFDANSDKGKEIFHLLTLLQRLCAQ 240
            KMIQS+ETELDSGRTEFEIADYMKQLTADIISRTEFD +SDKG +IFHLLTLLQRLCAQ
Sbjct: 181 NKMIQSMETELDSGRTEFEIADYMKQLTADIISRTEFDTDSDKGNQIFHLLTLLQRLCAQ 240

Query: 241 ASRHLCLPGSRFFPSKYNREIKSLKMEVERLLMEIIQSRKDGVEIGRSTCYGSDLLGMLL 300
           ASRHLCLPGSRF PSKYNREIKSLK EVERLLMEII+SRKDGVEIGRS+CYGSDLLGMLL
Sbjct: 241 ASRHLCLPGSRFVPSKYNREIKSLKKEVERLLMEIIESRKDGVEIGRSSCYGSDLLGMLL 300

Query: 301 NEMQRKREDNGLSLNLQLIMDECKTFFFAGHETTALLLTWTIMLLATNPNWQHKVRHEVN 360
           NEMQ+++     +LNLQLI+DECKTFFFAGHETTALLLTWTIMLLATNPNWQH VR EVN
Sbjct: 301 NEMQQRKNSTTTTLNLQLIIDECKTFFFAGHETTALLLTWTIMLLATNPNWQHMVRAEVN 360

Query: 361 QVCNNNGGTPSVEHLSKLTLLNMVINESLRLYPPATVLPRMAFEDIKVGDLEIPKGLSIW 420
           QVC  N  TPS++HLSKLTLLNMVINESLRLYPPATVLPRMA ED+K+GD++IPKGLSIW
Sbjct: 361 QVC--NARTPSIQHLSKLTLLNMVINESLRLYPPATVLPRMALEDMKLGDMDIPKGLSIW 420

Query: 421 IPVLAIHHSEELWGKDANEFKPERFASKPFSGGRFIPFASGPRNCIGQGFALMEAKIILA 480
           IPVLAIHHSEELWGKDAN+F P RFA+K F GGRFIPFA GPRNCIGQGFA+MEAKIILA
Sbjct: 421 IPVLAIHHSEELWGKDANQFNPNRFAAKAFGGGRFIPFAGGPRNCIGQGFAIMEAKIILA 480

Query: 481 MFLSKFSFTISDSYRHAPVSVLTIQPKYGVQVYLTPINS 502
           MFLSKFSFTISD+YRHAPV VLTIQPK+GVQV LTP +S
Sbjct: 481 MFLSKFSFTISDTYRHAPVVVLTIQPKHGVQVCLTPFSS 517

BLAST of Lsi05G003830 vs. TAIR 10
Match: AT5G38450.1 (cytochrome P450, family 735, subfamily A, polypeptide 1 )

HSP 1 Score: 707.2 bits (1824), Expect = 9.3e-204
Identity = 348/518 (67.18%), Postives = 419/518 (80.89%), Query Frame = 0

Query: 3   IVIFLTTLLVIFISSLAKIAYDTISCYWLTPRRIRKKMEKQGVRGPEPRPLLGNILDVAS 62
           ++  L +LLVIF++++ ++ YDTISCYWLTPRRI+K ME+QGV GP+PRPL GNIL++++
Sbjct: 2   LLTILKSLLVIFVTTILRVLYDTISCYWLTPRRIKKIMEQQGVTGPKPRPLTGNILEISA 61

Query: 63  LVAKSTANDMNSIDHDIVDRLLPHYTTWTKQYGKRFVYWNGTEPRLCLAETELIKEVLSK 122
           +V++S + D +SI HDIV RLLPHY  W+KQYGKRF+ WNGT+PRLCL ETELIKE+L K
Sbjct: 62  MVSQSASKDCDSIHHDIVGRLLPHYVAWSKQYGKRFIVWNGTDPRLCLTETELIKELLMK 121

Query: 123 NSNVCGKSWLQRQGTKHFIGKGLLMANGEDC------------------YAGMMVECTKK 182
           ++ V G+SWLQ+QGTK+FIG+GLLMANG+D                   YA  MVECT K
Sbjct: 122 HNGVSGRSWLQQQGTKNFIGRGLLMANGQDWHHQRHLAAPAFTGERLKGYARHMVECTSK 181

Query: 183 MIQSLETELDSGRTEFEIADYMKQLTADIISRTEFDANSDKGKEIFHLLTLLQRLCAQAS 242
           +++ L  E+  G  E EI + M +LTADIISRT+F ++ +KGKE+F+ LT+LQR CAQA+
Sbjct: 182 LVERLRKEVGEGANEVEIGEEMHKLTADIISRTKFGSSFEKGKELFNHLTVLQRRCAQAT 241

Query: 243 RHLCLPGSRFFPSKYNREIKSLKMEVERLLMEIIQSRKDGVEIGRSTCYGSDLLGMLLNE 302
           RHLC PGSRF PSKYNREIKSLK EVERLL+EIIQSR+D  E+GRS+ +G DLLG+LLNE
Sbjct: 242 RHLCFPGSRFLPSKYNREIKSLKKEVERLLIEIIQSRRDCAEMGRSSTHGDDLLGLLLNE 301

Query: 303 MQRKREDNGLSLNLQLIMDECKTFFFAGHETTALLLTWTIMLLATNPNWQHKVRHEVNQV 362
           M   + +N  + NLQLIMDECKTFFFAGHETTALLLTWT MLLA NP WQ KVR EV +V
Sbjct: 302 MDIDKNNNNNNNNLQLIMDECKTFFFAGHETTALLLTWTTMLLADNPTWQEKVREEVREV 361

Query: 363 CNNNGGTPSVEHLSKLTLLNMVINESLRLYPPATVLPRMAFEDIKVGDLEIPKGLSIWIP 422
              N G PSV+ LSKLT L+ VINESLRLYPPAT+LPRMAFED+K+GDL IPKGLSIWIP
Sbjct: 362 FGRN-GLPSVDQLSKLTSLSKVINESLRLYPPATLLPRMAFEDLKLGDLTIPKGLSIWIP 421

Query: 423 VLAIHHSEELWGKDANEFKPERFASKPFSGGR-FIPFASGPRNCIGQGFALMEAKIILAM 482
           VLAIHHSEELWGKDAN+F PERF  +PF+ GR FIPFA+GPRNCIGQ FALMEAKIILA 
Sbjct: 422 VLAIHHSEELWGKDANQFNPERFGGRPFASGRHFIPFAAGPRNCIGQQFALMEAKIILAT 481

Query: 483 FLSKFSFTISDSYRHAPVSVLTIQPKYGVQVYLTPINS 502
            +SKF+FTIS +YRHAP+ VLTI+PKYGVQV L P+ S
Sbjct: 482 LISKFNFTISKNYRHAPIVVLTIKPKYGVQVILKPLVS 518

BLAST of Lsi05G003830 vs. TAIR 10
Match: AT1G67110.1 (cytochrome P450, family 735, subfamily A, polypeptide 2 )

HSP 1 Score: 682.6 bits (1760), Expect = 2.5e-196
Identity = 342/519 (65.90%), Postives = 412/519 (79.38%), Query Frame = 0

Query: 1   MAIVIFLTTLLVIFISSLAKIAYDTISCYWLTPRRIRKKMEKQGVRGPEPRPLLGNILDV 60
           M + + L  +LVI ++ + ++ YD+I CY+LTPRRI+K ME+QG+ GP+PR L GNI+D+
Sbjct: 1   MMVTLVLKYVLVIVMTLILRVLYDSICCYFLTPRRIKKFMERQGITGPKPRLLTGNIIDI 60

Query: 61  ASLVAKSTANDMNSIDHDIVDRLLPHYTTWTKQYGKRFVYWNGTEPRLCLAETELIKEVL 120
           + +++ S +ND +SI H+IV RLLPHY +W+KQYGKRF+ WNGTEPRLCL ETE+IKE+L
Sbjct: 61  SKMLSHSASNDCSSIHHNIVPRLLPHYVSWSKQYGKRFIMWNGTEPRLCLTETEMIKELL 120

Query: 121 SKNSNVCGKSWLQRQGTKHFIGKGLLMANGE------------------DCYAGMMVECT 180
           +K++ V GKSWLQ+QGTK FIG+GLLMANGE                    YA  MVECT
Sbjct: 121 TKHNPVTGKSWLQQQGTKGFIGRGLLMANGEAWHHQRHMAAPAFTRDRLKGYAKHMVECT 180

Query: 181 KKMIQSLETELDSGRTEFEIADYMKQLTADIISRTEFDANSDKGKEIFHLLTLLQRLCAQ 240
           K M + L  E+     E EI + M++LTADIISRTEF ++ DKGKE+F LLT+LQRLCAQ
Sbjct: 181 KMMAERLRKEVGE---EVEIGEEMRRLTADIISRTEFGSSCDKGKELFSLLTVLQRLCAQ 240

Query: 241 ASRHLCLPGSRFFPSKYNREIKSLKMEVERLLMEIIQSRKDGVEIGRSTCYGSDLLGMLL 300
           A+RHLC PGSRF PSKYNREIKSLK EVERLLMEII SRKD VEIGRS+ YG DLLG+LL
Sbjct: 241 ATRHLCFPGSRFLPSKYNREIKSLKTEVERLLMEIIDSRKDSVEIGRSSSYGDDLLGLLL 300

Query: 301 NEMQRKREDNGLSLNLQLIMDECKTFFFAGHETTALLLTWTIMLLATNPNWQHKVRHEVN 360
           N+M    + N  +LN+Q+IMDECKTFFF GHETT+LLLTWT+MLLA NP WQ  VR EV 
Sbjct: 301 NQM----DSNKNNLNVQMIMDECKTFFFTGHETTSLLLTWTLMLLAHNPTWQDNVRDEVR 360

Query: 361 QVCNNNGGTPSVEHLSKLTLLNMVINESLRLYPPATVLPRMAFEDIKVGDLEIPKGLSIW 420
           QVC  + G PSVE LS LT LN VINESLRLYPPAT+LPRMAFEDIK+GDL IPKGLSIW
Sbjct: 361 QVCGQD-GVPSVEQLSSLTSLNKVINESLRLYPPATLLPRMAFEDIKLGDLIIPKGLSIW 420

Query: 421 IPVLAIHHSEELWGKDANEFKPERFASKPFSGGR-FIPFASGPRNCIGQGFALMEAKIIL 480
           IPVLAIHHS ELWG+DANEF PERF ++ F+  R F+PFA+GPRNCIGQ FA+MEAKIIL
Sbjct: 421 IPVLAIHHSNELWGEDANEFNPERFTTRSFASSRHFMPFAAGPRNCIGQTFAMMEAKIIL 480

Query: 481 AMFLSKFSFTISDSYRHAPVSVLTIQPKYGVQVYLTPIN 501
           AM +SKFSF IS++YRHAP+ VLTI+PKYGVQ+ L P++
Sbjct: 481 AMLVSKFSFAISENYRHAPIVVLTIKPKYGVQLVLKPLD 511

BLAST of Lsi05G003830 vs. TAIR 10
Match: AT2G46950.1 (cytochrome P450, family 709, subfamily B, polypeptide 2 )

HSP 1 Score: 339.0 bits (868), Expect = 6.7e-93
Identity = 191/492 (38.82%), Postives = 279/492 (56.71%), Query Frame = 0

Query: 33  PRRIRKKMEKQGVRGPEPRPLLGNILDVASLVAKSTANDMNSIDHDIVDRLLPHYTTWTK 92
           P  + ++ +KQG+ GP+ R L GN+ ++  +  ++    ++   +DIV R+LPH   W  
Sbjct: 88  PWMLSRRFKKQGISGPKYRILYGNLREIRKMKNEAKLMVLDPNSNDIVPRVLPHLQQWKS 147

Query: 93  QYGKRFVYWNGTEPRLCLAETELIKEVLSKNSNVCGKSWLQRQGTKHFIGKGLLMANGED 152
           QYG+ F+YW GT+PRLC+++ EL K++LS       KS  + +  K   G GL+  NG D
Sbjct: 148 QYGETFLYWQGTDPRLCISDHELAKQILSNKFVFFSKSKTKPEILK-LSGNGLIFVNGLD 207

Query: 153 ------------------CYAGMMVECTKKMIQSLETELDSGRTE--FEIADYMKQLTAD 212
                                 +MV+CT +M    + + +   TE    I+   K+LTAD
Sbjct: 208 WVRHRRILNPAFSMDKLKLMTQLMVDCTFRMFLEWKKQRNGVETEQFVLISREFKRLTAD 267

Query: 213 IISRTEFDANSDKGKEIFHLLTLLQRLCAQASRHLCLPGSRFFPSKYNREIKSLKMEVER 272
           II+   F ++  +G E+F     LQ+ CA A   L  PG ++ P+  N +I  L M+V  
Sbjct: 268 IIATAAFGSSYAEGIEVFKSQLELQKCCAAALTDLYFPGIQYLPTPSNLQIWKLDMKVNS 327

Query: 273 LLMEIIQSRKDGVEIGRSTCYGSDLLGMLLNEMQRKREDNGLSLNLQLIMDECKTFFFAG 332
            +  II +R        S  YG+DLLG++L        +  +S++   I++ECKTFFFAG
Sbjct: 328 SIKRIIDARL----TSESKDYGNDLLGIMLTAASSNESEKKMSID--EIIEECKTFFFAG 387

Query: 333 HETTALLLTWTIMLLATNPNWQHKVRHEVNQVCNNNGGTPSVEHLSKLTLLNMVINESLR 392
           HETTA LLTW+ MLL+ + +WQ K+R EV   C  +   P  E  SKL L+N V  ESLR
Sbjct: 388 HETTANLLTWSTMLLSLHQDWQEKLREEVFNECGKD-KIPDAETCSKLKLMNTVFMESLR 447

Query: 393 LYPPATVLPRMAFEDIKVGDLEIPKGLSIWIPVLAIHHSEELWGKDANEFKPERFASKPF 452
           LY P   L R+A ED+K+G+LEIPKG +I +P+  +H  + +WG DA++F P RFA+   
Sbjct: 448 LYGPVLNLLRLASEDMKLGNLEIPKGTTIILPIAKMHRDKAVWGSDADKFNPMRFANGLS 507

Query: 453 SGGR----FIPFASGPRNCIGQGFALMEAKIILAMFLSKFSFTISDSYRHAPVSVLTIQP 501
                    + F+ GPR CIGQ FA+MEAK +LAM L +F   +S  Y+HAP   LT+QP
Sbjct: 508 RAANHPNALLAFSMGPRACIGQNFAIMEAKTVLAMILQRFRLNLSADYKHAPADHLTLQP 567

BLAST of Lsi05G003830 vs. TAIR 10
Match: AT2G46960.2 (cytochrome P450, family 709, subfamily B, polypeptide 1 )

HSP 1 Score: 337.8 bits (865), Expect = 1.5e-92
Identity = 194/527 (36.81%), Postives = 297/527 (56.36%), Query Frame = 0

Query: 1   MAIVIFLTTLLVIFISSLAKIAYDTISCYWLTPRRIRKKMEKQGVRGPEPRPLLGNILDV 60
           M +VIFL  +++I I  L +I    +   W  P  + ++++ QG+ GP  R   GN+ ++
Sbjct: 1   MGLVIFLALIVLILIIGL-RIFKAFMILVW-HPFVLTRRLKNQGISGPNYRIFYGNLSEI 60

Query: 61  ASLVAKSTANDMNSIDHDIVDRLLPHYTTWTKQYGKRFVYWNGTEPRLCLAETELIKEVL 120
             +  +S  + ++   +DI  R+LPHY  W  QYG+ F+YWNGTEPR+C+++ EL K +L
Sbjct: 61  KKMKRESHLSILDPSSNDIFPRILPHYQKWMSQYGETFLYWNGTEPRICISDPELAKTML 120

Query: 121 SKNSNVCGKSWLQRQGTKHFIGKGLLMANGED------------------CYAGMMVECT 180
           S       KS  + +  K    KGL+   G D                      +MV+CT
Sbjct: 121 SNKLGFFVKSKARPEAVKLVGSKGLVFIEGADWVRHRRILNPAFSIDRLKIMTTVMVDCT 180

Query: 181 KKMIQSLETELDSGRTEF-----EIADYMKQLTADIISRTEFDANSDKGKEIFHLLTLLQ 240
            KM++    E     TE      E+ +  ++LTADII+ + F ++  +G E+F     L+
Sbjct: 181 LKMLEEWRKESTKEETEHPKIKKEMNEEFQRLTADIIATSAFGSSYVEGIEVFRSQMELK 240

Query: 241 RLCAQASRHLCLPGSRFFPSKYNREIKSLKMEVERLLMEIIQSRKDGVEIGRSTCYGSDL 300
           R    +   + +PG+++ P+  N  +  L+ +++  +  II SR     +   + YG DL
Sbjct: 241 RCYTTSLNQVSIPGTQYLPTPSNIRVWKLERKMDNSIKRIISSR-----LQSKSDYGDDL 300

Query: 301 LGMLLNEMQRKREDNGLSLNLQLIMDECKTFFFAGHETTALLLTWTIMLLATNPNWQHKV 360
           LG+LL     + ++  +S  ++ I+ EC+TFFF GHETT+ LL WT MLL+ + +WQ K+
Sbjct: 301 LGILLKAYNTEGKERKMS--IEEIIHECRTFFFGGHETTSNLLAWTTMLLSLHQDWQEKL 360

Query: 361 RHEVNQVCNNNGGTPSVEHLSKLTLLNMVINESLRLYPPATVLPRMAFEDIKVGDLEIPK 420
           R E+ + C     TP  E  SKL L+NMVI ESLRLY P + L R A  +IK+GDLEIPK
Sbjct: 361 REEIFKECGKE-KTPDSETFSKLKLMNMVIMESLRLYGPVSALAREASVNIKLGDLEIPK 420

Query: 421 GLSIWIPVLAIHHSEELWGKDANEFKPERFASKPFSGGR----FIPFASGPRNCIGQGFA 480
           G ++ IP+L +H  + LWG DA++F P RFA+            + F+ GPR CIGQ F 
Sbjct: 421 GTTVVIPLLKMHSDKTLWGSDADKFNPMRFANGVSRAANHPNALLAFSVGPRACIGQNFV 480

Query: 481 LMEAKIILAMFLSKFSF-TISDSYRHAPVSVLTIQPKYGVQVYLTPI 500
           ++EAK +L M L +F F ++ D Y+H PV  +TIQP+YG+ V L P+
Sbjct: 481 MIEAKTVLTMILQRFRFISLCDEYKHTPVDNVTIQPQYGLPVMLQPL 517

BLAST of Lsi05G003830 vs. TAIR 10
Match: AT5G52400.1 (cytochrome P450, family 715, subfamily A, polypeptide 1 )

HSP 1 Score: 315.5 bits (807), Expect = 7.9e-86
Identity = 189/527 (35.86%), Postives = 277/527 (52.56%), Query Frame = 0

Query: 5   IFLTTLLVIFISSLAKIAYDTISCYWLTPRRIRKKMEKQGVRGPEPRPLLGNILDVASLV 64
           IF    +V+      K+    + C W+ P R +KK+   G  GP P    GN+ D+  L 
Sbjct: 12  IFFKVFVVVICLMFLKL---FLRC-WIWPVRAQKKLRGNGFVGPAPSFPFGNLNDMKKLK 71

Query: 65  AKSTANDMNS----IDHDIVDRLLPHYTTWTKQYGKRFVYWNGTEPRLCLAETELIKEVL 124
             S   D +     I+HDI    LPH+  W ++YGK FVYW G EP + +A+ E +  V+
Sbjct: 72  MASVVVDNSKSSTIINHDIHSIALPHFARWQQEYGKVFVYWLGIEPFVYVADPEFL-SVM 131

Query: 125 SKNSNVCGKSW----LQRQGTKHFIGKGLLMANGED------------------CYAGMM 184
           SK   V GKSW    + ++  +   G GL+M  G+D                      MM
Sbjct: 132 SK--GVLGKSWGKPNVFKKDREPMFGTGLVMVEGDDWTRHRHIITPAFAPLNLKVMTNMM 191

Query: 185 VECTKKMIQSLETELDSGRTEFEIADYMKQLTADIISRTEFDANSDKGKEIFHLLTLLQR 244
           VE    M+     +++SG  EF++   +     +II++T F    + G ++   L  +Q 
Sbjct: 192 VESVSNMLDRWGIQINSGNPEFDMESEIIGTAGEIIAKTSFGVTGENGTQVLKNLRAVQF 251

Query: 245 LCAQASRHLCLPGSRFFPSKYNREIKSLKMEVERLLMEIIQSRKDGVEIGRSTCYGSDLL 304
               ++R++ +P S     K   + K L  E++ LL+  I  RK  + +      G DLL
Sbjct: 252 ALFNSNRYVGVPFSNILSYKQTVKAKGLGHEIDGLLLSFINKRK--ISLAEGDDQGHDLL 311

Query: 305 GMLLNEMQRKREDNGLSLNLQLIMDECKTFFFAGHETTALLLTWTIMLLATNPNWQHKVR 364
           GMLL      + D   +   + ++DECKTFFFAGHETTAL LTWT MLLA +P WQ  +R
Sbjct: 312 GMLL------KADQKGNFTAKELVDECKTFFFAGHETTALALTWTFMLLAIHPEWQDTIR 371

Query: 365 HEVNQVCNNNGGTPSVEH--LSKLTLLNMVINESLRLYPPATVLPRMAFEDIKVGDLEIP 424
            E+ +V     G   +E+  L+ L  ++ V+NE LRLYPPA    R A  DI+V    IP
Sbjct: 372 EEIREVI----GDSKIEYNKLAGLKKMSWVMNEVLRLYPPAPNAQRQARNDIEVNGRVIP 431

Query: 425 KGLSIWIPVLAIHHSEELWGKDANEFKPERFASKPFSGGR----FIPFASGPRNCIGQGF 484
            G +IWI V+A+HH  ELWG D NEFKPERF      G +    ++PF  G R CIG+  
Sbjct: 432 NGTNIWIDVVAMHHDVELWGDDVNEFKPERFDGNLHGGCKNKMGYMPFGFGGRMCIGRNL 491

Query: 485 ALMEAKIILAMFLSKFSFTISDSYRHAPVSVLTIQPKYGVQVYLTPI 500
             ME KI+L++ LS+F  ++S  YRH+P  +L+++P YG+ + + P+
Sbjct: 492 TTMEYKIVLSLVLSRFEISVSPGYRHSPTYMLSLRPGYGLPLIIRPL 519