Lsi05G003610 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi05G003610
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Descriptionagamous-like MADS-box protein AGL14
Locationchr05: 4597288 .. 4598034 (-)
RNA-Seq ExpressionLsi05G003610
SyntenyLsi05G003610
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGAAGAGGAAAATTGAGCCTCAATCTGATTCCGAATGAGAAATCTCGAATCTCAACCTTCAAAAAGAGGAAGAACAGTTTGATGAAGAAGGCTTACGAGCTTTCCACGCTTTGCGACGTTCGAACCTGCGTTCTCATCTATGGCCCTGCTCAATATCCTTCCCAATCGGACTTCCAATTTTACACTTGGCCTTCCAATCGAACCGACCTCGAAGCCATGATCCGTGCCTACAAGACCAACTCATCCTCTAATTTTTACAAACACAAGTGTTTCCGTTTGACTGATTTTTTCTCGGATCGGAAGAGGAAAATCGAGTCGGAGATGTGTAAACTTCATGAGAAATTTGAGGAAGTTATGTGCCCTAAGTGGGACGAGCAGCTTGAGCGTTTGTCAGAACACGAATTGAGAGAGTTCATGGAAGGACTCGATGTCAAAATTGAAGCCGCCAATCGAATGATCAATTTCATGGAAGCCGATCACGAAGACCTATTTCGCGAGTTGTTTCCAGAAAATACAGTTCAAATAGGAGGGGATTCCAATACTCCTCCTCCTCCTCCAGTACTGAATCCATCAGATTTGTTCATGATTGGGGCTAACCCTAATGAAGAAGATGTGGCGATGAATGAGTTCAATATTAGGTATGAAGAAGACAACTACATGGCTCATTACGTAAATCTGATGAACGATGAGTTTGATTATTGCAATAATATTGAGCATTTGAAGATGCCCACCGGTAACTTCTAA

mRNA sequence

ATGGGAAGAGGAAAATTGAGCCTCAATCTGATTCCGAATGAGAAATCTCGAATCTCAACCTTCAAAAAGAGGAAGAACAGTTTGATGAAGAAGGCTTACGAGCTTTCCACGCTTTGCGACGTTCGAACCTGCGTTCTCATCTATGGCCCTGCTCAATATCCTTCCCAATCGGACTTCCAATTTTACACTTGGCCTTCCAATCGAACCGACCTCGAAGCCATGATCCGTGCCTACAAGACCAACTCATCCTCTAATTTTTACAAACACAAGTGTTTCCGTTTGACTGATTTTTTCTCGGATCGGAAGAGGAAAATCGAGTCGGAGATGTGTAAACTTCATGAGAAATTTGAGGAAGTTATGTGCCCTAAGTGGGACGAGCAGCTTGAGCGTTTGTCAGAACACGAATTGAGAGAGTTCATGGAAGGACTCGATGTCAAAATTGAAGCCGCCAATCGAATGATCAATTTCATGGAAGCCGATCACGAAGACCTATTTCGCGAGTTGTTTCCAGAAAATACAGTTCAAATAGGAGGGGATTCCAATACTCCTCCTCCTCCTCCAGTACTGAATCCATCAGATTTGTTCATGATTGGGGCTAACCCTAATGAAGAAGATGTGGCGATGAATGAGTTCAATATTAGGTATGAAGAAGACAACTACATGGCTCATTACGTAAATCTGATGAACGATGAGTTTGATTATTGCAATAATATTGAGCATTTGAAGATGCCCACCGGTAACTTCTAA

Coding sequence (CDS)

ATGGGAAGAGGAAAATTGAGCCTCAATCTGATTCCGAATGAGAAATCTCGAATCTCAACCTTCAAAAAGAGGAAGAACAGTTTGATGAAGAAGGCTTACGAGCTTTCCACGCTTTGCGACGTTCGAACCTGCGTTCTCATCTATGGCCCTGCTCAATATCCTTCCCAATCGGACTTCCAATTTTACACTTGGCCTTCCAATCGAACCGACCTCGAAGCCATGATCCGTGCCTACAAGACCAACTCATCCTCTAATTTTTACAAACACAAGTGTTTCCGTTTGACTGATTTTTTCTCGGATCGGAAGAGGAAAATCGAGTCGGAGATGTGTAAACTTCATGAGAAATTTGAGGAAGTTATGTGCCCTAAGTGGGACGAGCAGCTTGAGCGTTTGTCAGAACACGAATTGAGAGAGTTCATGGAAGGACTCGATGTCAAAATTGAAGCCGCCAATCGAATGATCAATTTCATGGAAGCCGATCACGAAGACCTATTTCGCGAGTTGTTTCCAGAAAATACAGTTCAAATAGGAGGGGATTCCAATACTCCTCCTCCTCCTCCAGTACTGAATCCATCAGATTTGTTCATGATTGGGGCTAACCCTAATGAAGAAGATGTGGCGATGAATGAGTTCAATATTAGGTATGAAGAAGACAACTACATGGCTCATTACGTAAATCTGATGAACGATGAGTTTGATTATTGCAATAATATTGAGCATTTGAAGATGCCCACCGGTAACTTCTAA

Protein sequence

MGRGKLSLNLIPNEKSRISTFKKRKNSLMKKAYELSTLCDVRTCVLIYGPAQYPSQSDFQFYTWPSNRTDLEAMIRAYKTNSSSNFYKHKCFRLTDFFSDRKRKIESEMCKLHEKFEEVMCPKWDEQLERLSEHELREFMEGLDVKIEAANRMINFMEADHEDLFRELFPENTVQIGGDSNTPPPPPVLNPSDLFMIGANPNEEDVAMNEFNIRYEEDNYMAHYVNLMNDEFDYCNNIEHLKMPTGNF
Homology
BLAST of Lsi05G003610 vs. ExPASy Swiss-Prot
Match: Q9FIM0 (Agamous-like MADS-box protein AGL82 OS=Arabidopsis thaliana OX=3702 GN=AGL82 PE=1 SV=1)

HSP 1 Score: 80.1 bits (196), Expect = 3.9e-14
Identity = 55/171 (32.16%), Postives = 84/171 (49.12%), Query Frame = 0

Query: 6   LSLNLIPNEKSRISTFKKRKNSLMKKAYELSTLCDVRTCVLIYGPAQYPSQSDFQFYTWP 65
           + L  I N+K+RI+T+KKRK SL KKA E STLC V TC+++YGP +       +   WP
Sbjct: 6   VDLQRIANDKTRITTYKKRKASLYKKAQEFSTLCGVETCLIVYGPTKATDVVISEPEIWP 65

Query: 66  SNRTDLEAMIRAYKTNSSSNFYKHKCFRLTDFFSD---RKRKIESEMCKLHEKFEEVMCP 125
            + T + A+IR YK   S++  K     +  F +D       +  +  K   K+      
Sbjct: 66  KDETKVRAIIRKYKDTVSTSCRKET--NVETFVNDVGKGNEVVTKKRVKRENKYS----- 125

Query: 126 KWDEQLERLSEHELREFMEGLDVKI-EAANRMINFMEADHEDLFRELFPEN 173
            W+E+L++ S  +L      +D K+ EA  R    M   +       FP+N
Sbjct: 126 SWEEKLDKCSREQLHGIFCAVDSKLNEAVTRQERSMFRVNHQAMDTPFPQN 169

BLAST of Lsi05G003610 vs. ExPASy Swiss-Prot
Match: Q9FJK3 (Agamous-like MADS-box protein AGL80 OS=Arabidopsis thaliana OX=3702 GN=AGL80 PE=1 SV=1)

HSP 1 Score: 62.8 bits (151), Expect = 6.4e-09
Identity = 34/80 (42.50%), Postives = 49/80 (61.25%), Query Frame = 0

Query: 1  MGRGKLSLNLIPNEKSRISTFKKRKNSLMKKAYELSTLCDVRTCVLIYGPAQYPSQSDFQ 60
          M R K+ L  I N+ SR +TFKKRK  LMKK +ELSTLC +  C +IY P  Y +  +  
Sbjct: 1  MTRKKVKLAYISNDSSRKATFKKRKKGLMKKVHELSTLCGITACAIIYSP--YDTNPE-- 60

Query: 61 FYTWPSNRTDLEAMIRAYKT 81
             WPSN + ++ ++  ++T
Sbjct: 61 --VWPSN-SGVQRVVSEFRT 73

BLAST of Lsi05G003610 vs. ExPASy Swiss-Prot
Match: Q7XJK5 (Agamous-like MADS-box protein AGL90 OS=Arabidopsis thaliana OX=3702 GN=AGL90 PE=1 SV=2)

HSP 1 Score: 60.8 bits (146), Expect = 2.4e-08
Identity = 51/181 (28.18%), Postives = 86/181 (47.51%), Query Frame = 0

Query: 5   KLSLNLIPNEKSRISTFKKRKNSLMKKAYELSTLCDVRTCVLIYGPAQYPSQSDFQFYTW 64
           K+ L+LI NE+SR ++F KRKN + KK +ELSTLC V+ C LIY P     +S      W
Sbjct: 3   KVKLSLIANERSRKTSFMKRKNGIFKKLHELSTLCGVQACALIYSPFIPVPES------W 62

Query: 65  PSNR------TDLEAMIRAYKTNSSSNFYKHKCFRLTDFFSDRKRKIESEMCKLHEKFEE 124
           PS        +    M R  +T    +   H   R+T    ++ + + +E  +L  +   
Sbjct: 63  PSREGAKKVASKFLEMPRTARTRKMMDQETHLMERITK-AKEQLKNLAAENRELQVRRFM 122

Query: 125 VMCPKWDEQLERLSEHELREFMEGLDVKIEAANRMINFMEADHEDLFRELFPENTVQIGG 180
             C +      R    +L++ +  +++ ++  N  I  ++ + E L   + P  T +IG 
Sbjct: 123 FDCVEGKMSQYRYDAKDLQDLLSCMNLYLDQLNGRIESIKENGESLLSSVSPFPT-RIGV 175

BLAST of Lsi05G003610 vs. ExPASy Swiss-Prot
Match: O64703 (Agamous-like MADS-box protein AGL29 OS=Arabidopsis thaliana OX=3702 GN=AGL29 PE=2 SV=1)

HSP 1 Score: 59.3 bits (142), Expect = 7.1e-08
Identity = 46/167 (27.54%), Postives = 79/167 (47.31%), Query Frame = 0

Query: 1   MGRGKLSLNLIPNEKSRISTFKKRKNSLMKKAYELSTLCDVRTCVLIYGPAQYPSQSDFQ 60
           MGR K+ + ++ +  +R  TF KR+  L KKA EL+TLC+    ++++ P   P      
Sbjct: 1   MGRRKIKMEMVQDMNTRQVTFSKRRTGLFKKASELATLCNAELGIVVFSPGGKPFS---- 60

Query: 61  FYTWPSNRTDLEAMIRAYKTNSS-----SNFYKHKCFRLTDFFSDRKRKIESEM---CKL 120
            Y  P+  +  E  +R Y  + S     S  Y+ K  RL++      +++E+E     K 
Sbjct: 61  -YGKPNLDSVAERFMREYDDSDSGDEEKSGNYRPKLKRLSERLDLLNQEVEAEKERGEKS 120

Query: 121 HEKFEEVMCPKWDEQLERLSEHELREFMEGLDVKIEAANRMINFMEA 160
            EK E     ++ E +E L+  EL E+ + L          +N ++A
Sbjct: 121 QEKLESAGDERFKESIETLTLDELNEYKDRLQTVHGRIEGQVNHLQA 162

BLAST of Lsi05G003610 vs. ExPASy Swiss-Prot
Match: Q07474 (Floral homeotic protein PMADS 2 OS=Petunia hybrida OX=4102 GN=PMADS2 PE=2 SV=1)

HSP 1 Score: 59.3 bits (142), Expect = 7.1e-08
Identity = 47/184 (25.54%), Postives = 86/184 (46.74%), Query Frame = 0

Query: 1   MGRGKLSLNLIPNEKSRISTFKKRKNSLMKKAYELSTLCDVRTCVLIYGPAQYPSQSDFQ 60
           MGRGK+ +  I N  +R  T+ KR+N ++KKA E++ LCD +  ++I+G       +  +
Sbjct: 1   MGRGKIEIKRIENSSNRQVTYSKRRNGIIKKAKEITVLCDAKVSLIIFG-------NSGK 60

Query: 61  FYTWPSNRTDLEAMIRAYKTNSSSNFYKHKCFRLTDFFSDRKRKIESEMCKL-HEKFEEV 120
            + + S  T L  M+  Y+  S    +  K   L++     K++ ++   KL H K E++
Sbjct: 61  MHEYCSPSTTLPDMLDGYQKTSGRRLWDAKHENLSNEIDRIKKENDNMQVKLRHLKGEDI 120

Query: 121 MCPKWDEQLERLSEHELREFMEGLDVKIEAANRMI----NFMEADHEDLFRELFPENTVQ 180
                 E +  + E  L   +  +  K     RM+      +E +H+ L   L  +    
Sbjct: 121 NSLNHKELM--VLEEGLTNGLSSISAKQSEILRMVRKNDQILEEEHKQLQYALHQKEMAA 175

BLAST of Lsi05G003610 vs. ExPASy TrEMBL
Match: A0A6J1F4C8 (agamous-like MADS-box protein AGL14 OS=Cucurbita moschata OX=3662 GN=LOC111442271 PE=4 SV=1)

HSP 1 Score: 275.4 bits (703), Expect = 2.3e-70
Identity = 154/260 (59.23%), Postives = 189/260 (72.69%), Query Frame = 0

Query: 1   MGRGKLSLNLIPNEKSRISTFKKRKNSLMKKAYELSTLCDVRTCVLIYGPA----QYPSQ 60
           M RGKLS NLI N KSR STFK+RKNSLMKKAYELSTLCDVRTCVLI GP     + PS 
Sbjct: 1   MVRGKLSFNLIRNRKSRCSTFKQRKNSLMKKAYELSTLCDVRTCVLINGPGFAPDEDPSL 60

Query: 61  SDFQFYTWPSNRTDLEAMIRAYKTNSSSNFYKHKCFRLTDFFSDRKRKIESEMCKLHEKF 120
           S  +F+TWPSNR+++E MI AYK N + NF K KCF L+D+FSDRKRK+ +EMCK  EK 
Sbjct: 61  SSVEFHTWPSNRSEVEHMIHAYK-NGNCNFQKRKCFDLSDYFSDRKRKLVTEMCKHREKV 120

Query: 121 EEVMCPKWDEQLERLSEHELREFMEGLDVKIEAANRMINFMEADHEDLFRELFP----EN 180
            ++M P WDE+L+ LSE +LREFM GLD +IEAA RM++FM+AD+E+LF  +F     EN
Sbjct: 121 GKIMWPDWDERLDCLSEQQLREFMNGLDSRIEAAGRMVDFMDADNENLF-GMFSCEEYEN 180

Query: 181 TVQIGGDSNTPPPPPVLNPSDLFMIGANPNEEDVAMN----EFNIRYEEDNYMAHYVNLM 240
            VQ+GGDS+TP PP V   S   +   NPN+ ++  N    EF +  E++NYMA Y NLM
Sbjct: 181 VVQMGGDSSTPVPPLVELESPDSLNSPNPNKHEIGCNFENLEFLLEDEDENYMALYANLM 240

Query: 241 NDEFDYCNNIEHLKMPTGNF 249
           NDEFD CN+IE   + TGNF
Sbjct: 241 NDEFDECNDIEQW-LRTGNF 257

BLAST of Lsi05G003610 vs. ExPASy TrEMBL
Match: A0A6J1J5U6 (agamous-like MADS-box protein AGL14 OS=Cucurbita maxima OX=3661 GN=LOC111482037 PE=4 SV=1)

HSP 1 Score: 265.4 bits (677), Expect = 2.4e-67
Identity = 154/262 (58.78%), Postives = 189/262 (72.14%), Query Frame = 0

Query: 1   MGRGKLSLNLIPNEKSRISTFKKRKNSLMKKAYELSTLCDVRTCVLIYGPA----QYPSQ 60
           M RGKLS NLI N KSR STFK+RKNSLMKKAYELSTLCDVRTCVLI GP     + PS 
Sbjct: 1   MVRGKLSFNLIRNRKSRCSTFKQRKNSLMKKAYELSTLCDVRTCVLINGPGFAPDEDPSL 60

Query: 61  SDFQFYTWPSNRTDLEAMIRAYKTNSSSNFYKHKCFRLTDFFSDRKRKIESEMCKLHEKF 120
           S  +F+TWPSNR+++E MI AYK N + N  K KCF L+D+FSDRKRK+ +EMCK  EK 
Sbjct: 61  SSVEFHTWPSNRSEVEHMIHAYK-NGNCNLQKRKCFDLSDYFSDRKRKLVTEMCKHREKV 120

Query: 121 EEVMCPKWDEQLERLSEHELREFMEGLDVKIEAANRMINFMEADHEDLFRELFP----EN 180
            ++M P WDE+L+ LSE +LREFM GLD +IEAA RMI+FM+AD+E+LF  +F     EN
Sbjct: 121 GKIMWPDWDERLDCLSEQQLREFMNGLDSRIEAAGRMIDFMDADNENLF-GMFSCEEYEN 180

Query: 181 TVQIGGDSNTPPPPPV-LNPSD-LFMIGANPNEEDVAMNEFNIRY----EEDNYMAHYVN 240
            VQ+GGDS+TP    V L  +D L     NP+E+++  N  N+ Y    E++NYMA Y N
Sbjct: 181 VVQMGGDSSTPVLSLVELESTDSLNSPNPNPDEDEIGCNFENLEYLLEDEDENYMALYAN 240

Query: 241 LMNDEFDYCNNIEHLKMPTGNF 249
           LMNDEFD CN+IE   + TGNF
Sbjct: 241 LMNDEFDECNDIEQW-LRTGNF 259

BLAST of Lsi05G003610 vs. ExPASy TrEMBL
Match: A0A6J1CQM2 (uncharacterized protein LOC111013282 OS=Momordica charantia OX=3673 GN=LOC111013282 PE=4 SV=1)

HSP 1 Score: 159.8 bits (403), Expect = 1.4e-35
Identity = 98/179 (54.75%), Postives = 115/179 (64.25%), Query Frame = 0

Query: 1   MGRGKLSLNLIPNEKSRISTFKKRKNSLMKKAYELSTLCDVRTCVLIYGPA--QYPSQSD 60
           MGRGKLSL LI + KSR +TF+KR  SLMKKA+ELSTLCDVRTCVLI+GPA  Q   +S 
Sbjct: 1   MGRGKLSLKLISSHKSRTATFRKRTKSLMKKAFELSTLCDVRTCVLIHGPAPPQDGDRSV 60

Query: 61  FQFYTWPSNRTDLEAMIRAYKTNSSSNFYKHKCFRLTDFFSDRKRKIESEMCKLHEKFEE 120
            + +TWP NR ++E +IRAYKTN        K F L DFFS R +KIE E  K     EE
Sbjct: 61  VESHTWPPNREEIEGIIRAYKTNYLRK-PSMKSFGLYDFFSGRMKKIEKERAKFS---EE 120

Query: 121 VMCPKWDEQLERLSEHELREFMEGLDVKIEAANRMINFMEA----DHEDLFRELFPENT 174
           +    WDE+L  LSE +LR FM+ L  KIEAA  MI FME     D   +  ELF E T
Sbjct: 121 IKYAGWDERLNCLSEQQLRLFMDNLGSKIEAAEGMIGFMEGVSAIDSAGVSTELFEEKT 175

BLAST of Lsi05G003610 vs. ExPASy TrEMBL
Match: A0A6J1D6T5 (floral homeotic protein DEFICIENS-like OS=Momordica charantia OX=3673 GN=LOC111017467 PE=4 SV=1)

HSP 1 Score: 157.5 bits (397), Expect = 7.1e-35
Identity = 86/163 (52.76%), Postives = 110/163 (67.48%), Query Frame = 0

Query: 1   MGRGKLSLNLIPNEKSRISTFKKRKNSLMKKAYELSTLCDVRTCVLIYGPAQYPSQSDFQ 60
           MGRGKLS+ +IPNEKSR +TF KRK SLM+KAYELSTLCDVRTCV + GP Q   QS  +
Sbjct: 1   MGRGKLSMTMIPNEKSRKTTFFKRKKSLMRKAYELSTLCDVRTCVFVCGPNQ-NDQSSVE 60

Query: 61  FYTWPSNRTDLEAMIRAYKTNSSSNFYKHKCFRLTDFFSDRKRKIESEMCKLHEKFEEVM 120
            +TWP +R D++ MI +Y+ N        K F L DFFS+RK+KIES++ KLH+      
Sbjct: 61  VHTWPPSRDDVDDMIASYRANCLHK-RAGKPFDLLDFFSERKKKIESDISKLHKDLARAK 120

Query: 121 CPKWDEQLERLSEHELREFMEGLDVKIEAANRMINFMEADHED 164
            PKWDE+L+ L E +LR  ME LD K++A  R I     + E+
Sbjct: 121 FPKWDERLDYLLEDQLRVLMEELDSKLDATKRKIEIAMENLEE 161

BLAST of Lsi05G003610 vs. ExPASy TrEMBL
Match: A0A6J1G795 (floral homeotic protein FBP1-like OS=Cucurbita moschata OX=3662 GN=LOC111451466 PE=4 SV=1)

HSP 1 Score: 157.5 bits (397), Expect = 7.1e-35
Identity = 86/158 (54.43%), Postives = 107/158 (67.72%), Query Frame = 0

Query: 1   MGRGKLSLNLIPNEKSRISTFKKRKNSLMKKAYELSTLCDVRTCVLIYGPAQYPSQSDFQ 60
           MGRGKLS+ LI NEKSR +TF KRKNSLM+KAYELSTLCDVRTCV+++GP Q        
Sbjct: 1   MGRGKLSMKLISNEKSRKTTFSKRKNSLMRKAYELSTLCDVRTCVIVHGPNQ---SDQSP 60

Query: 61  FYTWPSNRTDLEAMIRAYKTNSSSNFYKHKCFRLTDFFSDRKRKIESEMCKLHEKFEEVM 120
            +TWPS+R  +  MI +YK+N        K F L DFFSDRK+KIES+M KL +   +  
Sbjct: 61  IHTWPSSRDSVNDMIASYKSNCLHK-RTRKSFNLFDFFSDRKKKIESDMSKLRKDVAKAR 120

Query: 121 CPKWDEQLERLSEHELREFMEGLDVKIEAANRMINFME 159
            PKWDE+LE L E +LR  M  +D K++A  R I  +E
Sbjct: 121 YPKWDERLEYLMEEQLRVLMIEIDSKLDATKRRIENVE 154

BLAST of Lsi05G003610 vs. NCBI nr
Match: XP_038887059.1 (agamous-like MADS-box protein AGL82, partial [Benincasa hispida])

HSP 1 Score: 307.4 bits (786), Expect = 1.1e-79
Identity = 176/243 (72.43%), Postives = 191/243 (78.60%), Query Frame = 0

Query: 1   MGRGKLSLNLIPNEKSRISTFKKRKNSLMKKAYELSTLCDVRTCVLIYGPA----QYPSQ 60
           MGRGKLSLNLIPN KSRISTFKKRKNSLMKKAYELSTLCDV+TCVL++GPA    + PSQ
Sbjct: 1   MGRGKLSLNLIPNRKSRISTFKKRKNSLMKKAYELSTLCDVQTCVLVHGPASLPTEDPSQ 60

Query: 61  SDFQFYTWPSNRTDLEAMIRAYKTNSSSNFYKHKCFRLTDFFSDRKRKIESEMCKLHEKF 120
           SD +F+TWPS+RT LEAMIRAYKT  S NF K KCF LTD+FSDRKRKIESE CKL EKF
Sbjct: 61  SDLEFHTWPSDRTHLEAMIRAYKTTRSCNFQKCKCFGLTDYFSDRKRKIESETCKLREKF 120

Query: 121 EEVMCPKWDEQLERLSEHELREFMEGLDVKIEAANRMINFME-ADHEDLFRELFPENTVQ 180
              MCPKWDE L+RLSEHELREFMEGLD KIEAAN MI+FME ADHE+LF EL  EN+VQ
Sbjct: 121 ---MCPKWDEWLDRLSEHELREFMEGLDAKIEAANGMIDFMELADHENLFGELCSENSVQ 180

Query: 181 IGGDSNTPPPPPVLNPS--DLFMIGANPNEEDV-AMNEF-NIRY--------EEDNYMAH 227
           +G DS     P VL PS  DLF  GAN  EEDV A++E  NIRY        EEDNYMAH
Sbjct: 181 MGEDS----IPQVLIPSVDDLFATGANHTEEDVMALDEIDNIRYLQMLLGEEEEDNYMAH 236

BLAST of Lsi05G003610 vs. NCBI nr
Match: XP_038887053.1 (agamous-like MADS-box protein AGL82, partial [Benincasa hispida])

HSP 1 Score: 288.1 bits (736), Expect = 7.2e-74
Identity = 160/213 (75.12%), Postives = 173/213 (81.22%), Query Frame = 0

Query: 1   MGRGKLSLNLIPNEKSRISTFKKRKNSLMKKAYELSTLCDVRTCVLIYGPA----QYPSQ 60
           MGRGKLSLNLIPN KSRISTFKKRKNSLMKKAYELSTLCDV+TCVL++GPA    + PSQ
Sbjct: 1   MGRGKLSLNLIPNRKSRISTFKKRKNSLMKKAYELSTLCDVQTCVLVHGPASLPTEDPSQ 60

Query: 61  SDFQFYTWPSNRTDLEAMIRAYKTNSSSNFYKHKCFRLTDFFSDRKRKIESEMCKLHEKF 120
           SD +F+TWPS+RT LEAMIRAYKT  S NF K KCF LTD+FSDRKRKIESE CKL EKF
Sbjct: 61  SDPEFHTWPSDRTHLEAMIRAYKTTRSCNFQKCKCFGLTDYFSDRKRKIESETCKLREKF 120

Query: 121 EEVMCPKWDEQLERLSEHELREFMEGLDVKIEAANRMINFME-ADHEDLFRELFPENTVQ 180
              MCPKWDE L+RLSEHELREFMEGLD KIEAAN MI+FME ADHE+LF EL  EN+VQ
Sbjct: 121 ---MCPKWDEWLDRLSEHELREFMEGLDAKIEAANGMIDFMELADHENLFGELCSENSVQ 180

Query: 181 IGGDSNTPPPPPVLNPS--DLFMIGANPNEEDV 207
           +G DS     P VL PS  DLF  GAN  EEDV
Sbjct: 181 MGEDS----IPQVLIPSVDDLFATGANHTEEDV 206

BLAST of Lsi05G003610 vs. NCBI nr
Match: XP_022935361.1 (agamous-like MADS-box protein AGL14 [Cucurbita moschata])

HSP 1 Score: 275.4 bits (703), Expect = 4.8e-70
Identity = 154/260 (59.23%), Postives = 189/260 (72.69%), Query Frame = 0

Query: 1   MGRGKLSLNLIPNEKSRISTFKKRKNSLMKKAYELSTLCDVRTCVLIYGPA----QYPSQ 60
           M RGKLS NLI N KSR STFK+RKNSLMKKAYELSTLCDVRTCVLI GP     + PS 
Sbjct: 1   MVRGKLSFNLIRNRKSRCSTFKQRKNSLMKKAYELSTLCDVRTCVLINGPGFAPDEDPSL 60

Query: 61  SDFQFYTWPSNRTDLEAMIRAYKTNSSSNFYKHKCFRLTDFFSDRKRKIESEMCKLHEKF 120
           S  +F+TWPSNR+++E MI AYK N + NF K KCF L+D+FSDRKRK+ +EMCK  EK 
Sbjct: 61  SSVEFHTWPSNRSEVEHMIHAYK-NGNCNFQKRKCFDLSDYFSDRKRKLVTEMCKHREKV 120

Query: 121 EEVMCPKWDEQLERLSEHELREFMEGLDVKIEAANRMINFMEADHEDLFRELFP----EN 180
            ++M P WDE+L+ LSE +LREFM GLD +IEAA RM++FM+AD+E+LF  +F     EN
Sbjct: 121 GKIMWPDWDERLDCLSEQQLREFMNGLDSRIEAAGRMVDFMDADNENLF-GMFSCEEYEN 180

Query: 181 TVQIGGDSNTPPPPPVLNPSDLFMIGANPNEEDVAMN----EFNIRYEEDNYMAHYVNLM 240
            VQ+GGDS+TP PP V   S   +   NPN+ ++  N    EF +  E++NYMA Y NLM
Sbjct: 181 VVQMGGDSSTPVPPLVELESPDSLNSPNPNKHEIGCNFENLEFLLEDEDENYMALYANLM 240

Query: 241 NDEFDYCNNIEHLKMPTGNF 249
           NDEFD CN+IE   + TGNF
Sbjct: 241 NDEFDECNDIEQW-LRTGNF 257

BLAST of Lsi05G003610 vs. NCBI nr
Match: XP_023528957.1 (agamous-like MADS-box protein AGL14 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 273.5 bits (698), Expect = 1.8e-69
Identity = 155/262 (59.16%), Postives = 189/262 (72.14%), Query Frame = 0

Query: 1   MGRGKLSLNLIPNEKSRISTFKKRKNSLMKKAYELSTLCDVRTCVLIYGPA----QYPSQ 60
           M RGKLS NLI N KSR STFK+RKNSLMKKAYELSTLCDVRTCVLI GP     + PS 
Sbjct: 1   MVRGKLSFNLIRNRKSRCSTFKQRKNSLMKKAYELSTLCDVRTCVLINGPGFAPDEDPSL 60

Query: 61  SDFQFYTWPSNRTDLEAMIRAYKTNSSSNFYKHKCFRLTDFFSDRKRKIESEMCKLHEKF 120
           S  +F+TWPSNR+++E MI AYK N + N  K KCF L+D+FSDRKRK+ +EMCK  EK 
Sbjct: 61  SSVEFHTWPSNRSEVEHMIHAYK-NGNCNLQKRKCFDLSDYFSDRKRKLVTEMCKHREKV 120

Query: 121 EEVMCPKWDEQLERLSEHELREFMEGLDVKIEAANRMINFMEADHEDLFRELFP----EN 180
            ++M P WDE+L+ LSE +LREFM GLD +IEAA RMI+FM+AD+E+LF  +F     EN
Sbjct: 121 GKIMWPDWDERLDCLSEQQLREFMNGLDSRIEAAGRMIDFMDADNENLF-GMFSCEEYEN 180

Query: 181 TVQIGGDSNTPPPPPV--LNPSDLFMIGANPNEEDVAMN----EFNIRYEEDNYMAHYVN 240
            VQ+GGDS+TP PP V   +P  L     NPNE+++  N    EF +  E++NYMA Y N
Sbjct: 181 VVQMGGDSSTPVPPLVELESPDSLNTPNPNPNEDEIGCNFENLEFLLEDEDENYMALYAN 240

Query: 241 LMNDEFDYCNNIEHLKMPTGNF 249
           LMNDE D CN+IE   + TGNF
Sbjct: 241 LMNDELDECNDIEQW-LRTGNF 259

BLAST of Lsi05G003610 vs. NCBI nr
Match: KAG6581223.1 (Agamous-like MADS-box protein AGL82, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 265.4 bits (677), Expect = 5.0e-67
Identity = 154/264 (58.33%), Postives = 187/264 (70.83%), Query Frame = 0

Query: 1   MGRGKLSLNLIPNEKSRISTFKKRKNSLMKKAYELSTLCDVRTCVLIYGPA----QYPSQ 60
           M RGKLS NLI N KSR STFK+RKNSLMKKAYELSTLCDVRTCVLI GP     + PS 
Sbjct: 1   MVRGKLSFNLIRNRKSRCSTFKQRKNSLMKKAYELSTLCDVRTCVLINGPGFAPDEDPSL 60

Query: 61  SDFQFYTWPSNRTDLEAMIRAYKTNSSSNFYKHKCFRLTDFFSDRKRKIESEMCKLHEKF 120
              +F+TWPSNR+++E MI AYK N + N  K KCF L+D+FSDRKRK+ +EMCK  EK 
Sbjct: 61  FSVEFHTWPSNRSEVEHMIHAYK-NGNCNLQKRKCFDLSDYFSDRKRKLVTEMCKHREKV 120

Query: 121 EEVMCPKWDEQLERLSEHELREFMEGLDVKIEAANRMINFMEADHEDLFRELFP----EN 180
            ++M P WDE+L+ LSE +LREFM GLD +IEAA RMI+FM+AD+E+LF  +F     EN
Sbjct: 121 GKIMWPDWDERLDCLSEQQLREFMNGLDSRIEAAGRMIDFMDADNENLF-GMFSCEEYEN 180

Query: 181 TVQIGGDSNTPPPPPV--LNPSDLFM--IGANPNEEDVAMN----EFNIRYEEDNYMAHY 240
            VQ+GGDS+TP PP V   +P  L       NPNE ++  N    EF +  E++NYMA Y
Sbjct: 181 VVQMGGDSSTPVPPLVELESPDSLNSPNPNPNPNEHEIGCNFENLEFLLEDEDENYMALY 240

Query: 241 VNLMNDEFDYCNNIEHLKMPTGNF 249
            NLMNDE D CN+IE   + TGNF
Sbjct: 241 ANLMNDELDECNDIEQW-LRTGNF 261

BLAST of Lsi05G003610 vs. TAIR 10
Match: AT5G58890.1 (AGAMOUS-like 82 )

HSP 1 Score: 80.1 bits (196), Expect = 2.8e-15
Identity = 55/171 (32.16%), Postives = 84/171 (49.12%), Query Frame = 0

Query: 6   LSLNLIPNEKSRISTFKKRKNSLMKKAYELSTLCDVRTCVLIYGPAQYPSQSDFQFYTWP 65
           + L  I N+K+RI+T+KKRK SL KKA E STLC V TC+++YGP +       +   WP
Sbjct: 6   VDLQRIANDKTRITTYKKRKASLYKKAQEFSTLCGVETCLIVYGPTKATDVVISEPEIWP 65

Query: 66  SNRTDLEAMIRAYKTNSSSNFYKHKCFRLTDFFSD---RKRKIESEMCKLHEKFEEVMCP 125
            + T + A+IR YK   S++  K     +  F +D       +  +  K   K+      
Sbjct: 66  KDETKVRAIIRKYKDTVSTSCRKET--NVETFVNDVGKGNEVVTKKRVKRENKYS----- 125

Query: 126 KWDEQLERLSEHELREFMEGLDVKI-EAANRMINFMEADHEDLFRELFPEN 173
            W+E+L++ S  +L      +D K+ EA  R    M   +       FP+N
Sbjct: 126 SWEEKLDKCSREQLHGIFCAVDSKLNEAVTRQERSMFRVNHQAMDTPFPQN 169

BLAST of Lsi05G003610 vs. TAIR 10
Match: AT5G55690.1 (MADS-box transcription factor family protein )

HSP 1 Score: 80.1 bits (196), Expect = 2.8e-15
Identity = 52/157 (33.12%), Postives = 85/157 (54.14%), Query Frame = 0

Query: 1   MGRGKLSLNLIPNEKSRISTFKKRKNSLMKKAYELSTLCDVRTCVLIYGPAQYPSQSDFQ 60
           MGR  + +  I NEK+RI+T+KKRK  L KKA E STLC V TCV++YGP++   +   +
Sbjct: 1   MGRKMVKMTRITNEKTRITTYKKRKACLYKKASEFSTLCGVDTCVIVYGPSRAGDEMVME 60

Query: 61  FYTWPSNRTDLEAMIRAYKTNSSSNFYKHKCFRLTDFFSDRKRKIESEMCKLHEKFEEVM 120
              WP + + +  ++  Y+  +SS+    K + + +       K+E     +  K+    
Sbjct: 61  PELWPKDGSKVREILTKYRDTASSSC--TKTYTVQECLEKNNTKVEKP--TIATKY---- 120

Query: 121 CPKWDEQLERLSEHELREFMEGLDVKI-EAANRMINF 157
            P WD++L++ S ++L      ++ KI EA NR   F
Sbjct: 121 -PTWDKKLDQCSLNDLYAVFMAVENKIQEATNRNQTF 148

BLAST of Lsi05G003610 vs. TAIR 10
Match: AT5G26580.1 (AGAMOUS-like-34 )

HSP 1 Score: 63.5 bits (153), Expect = 2.7e-10
Identity = 31/50 (62.00%), Postives = 37/50 (74.00%), Query Frame = 0

Query: 1  MGRGKLSLNLIPNEKSRISTFKKRKNSLMKKAYELSTLCDVRTCVLIYGP 51
          MG  K+ L+LI NE SR ++F KRKN +MKK YELSTLC V+ C LIY P
Sbjct: 1  MGMKKVKLSLIANEISRETSFMKRKNGIMKKLYELSTLCGVQACTLIYSP 50

BLAST of Lsi05G003610 vs. TAIR 10
Match: AT5G48670.1 (AGAMOUS-like 80 )

HSP 1 Score: 62.8 bits (151), Expect = 4.6e-10
Identity = 34/80 (42.50%), Postives = 49/80 (61.25%), Query Frame = 0

Query: 1  MGRGKLSLNLIPNEKSRISTFKKRKNSLMKKAYELSTLCDVRTCVLIYGPAQYPSQSDFQ 60
          M R K+ L  I N+ SR +TFKKRK  LMKK +ELSTLC +  C +IY P  Y +  +  
Sbjct: 1  MTRKKVKLAYISNDSSRKATFKKRKKGLMKKVHELSTLCGITACAIIYSP--YDTNPE-- 60

Query: 61 FYTWPSNRTDLEAMIRAYKT 81
             WPSN + ++ ++  ++T
Sbjct: 61 --VWPSN-SGVQRVVSEFRT 73

BLAST of Lsi05G003610 vs. TAIR 10
Match: AT5G27960.1 (AGAMOUS-like 90 )

HSP 1 Score: 60.8 bits (146), Expect = 1.7e-09
Identity = 51/181 (28.18%), Postives = 86/181 (47.51%), Query Frame = 0

Query: 5   KLSLNLIPNEKSRISTFKKRKNSLMKKAYELSTLCDVRTCVLIYGPAQYPSQSDFQFYTW 64
           K+ L+LI NE+SR ++F KRKN + KK +ELSTLC V+ C LIY P     +S      W
Sbjct: 3   KVKLSLIANERSRKTSFMKRKNGIFKKLHELSTLCGVQACALIYSPFIPVPES------W 62

Query: 65  PSNR------TDLEAMIRAYKTNSSSNFYKHKCFRLTDFFSDRKRKIESEMCKLHEKFEE 124
           PS        +    M R  +T    +   H   R+T    ++ + + +E  +L  +   
Sbjct: 63  PSREGAKKVASKFLEMPRTARTRKMMDQETHLMERITK-AKEQLKNLAAENRELQVRRFM 122

Query: 125 VMCPKWDEQLERLSEHELREFMEGLDVKIEAANRMINFMEADHEDLFRELFPENTVQIGG 180
             C +      R    +L++ +  +++ ++  N  I  ++ + E L   + P  T +IG 
Sbjct: 123 FDCVEGKMSQYRYDAKDLQDLLSCMNLYLDQLNGRIESIKENGESLLSSVSPFPT-RIGV 175

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9FIM03.9e-1432.16Agamous-like MADS-box protein AGL82 OS=Arabidopsis thaliana OX=3702 GN=AGL82 PE=... [more]
Q9FJK36.4e-0942.50Agamous-like MADS-box protein AGL80 OS=Arabidopsis thaliana OX=3702 GN=AGL80 PE=... [more]
Q7XJK52.4e-0828.18Agamous-like MADS-box protein AGL90 OS=Arabidopsis thaliana OX=3702 GN=AGL90 PE=... [more]
O647037.1e-0827.54Agamous-like MADS-box protein AGL29 OS=Arabidopsis thaliana OX=3702 GN=AGL29 PE=... [more]
Q074747.1e-0825.54Floral homeotic protein PMADS 2 OS=Petunia hybrida OX=4102 GN=PMADS2 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1F4C82.3e-7059.23agamous-like MADS-box protein AGL14 OS=Cucurbita moschata OX=3662 GN=LOC11144227... [more]
A0A6J1J5U62.4e-6758.78agamous-like MADS-box protein AGL14 OS=Cucurbita maxima OX=3661 GN=LOC111482037 ... [more]
A0A6J1CQM21.4e-3554.75uncharacterized protein LOC111013282 OS=Momordica charantia OX=3673 GN=LOC111013... [more]
A0A6J1D6T57.1e-3552.76floral homeotic protein DEFICIENS-like OS=Momordica charantia OX=3673 GN=LOC1110... [more]
A0A6J1G7957.1e-3554.43floral homeotic protein FBP1-like OS=Cucurbita moschata OX=3662 GN=LOC111451466 ... [more]
Match NameE-valueIdentityDescription
XP_038887059.11.1e-7972.43agamous-like MADS-box protein AGL82, partial [Benincasa hispida][more]
XP_038887053.17.2e-7475.12agamous-like MADS-box protein AGL82, partial [Benincasa hispida][more]
XP_022935361.14.8e-7059.23agamous-like MADS-box protein AGL14 [Cucurbita moschata][more]
XP_023528957.11.8e-6959.16agamous-like MADS-box protein AGL14 [Cucurbita pepo subsp. pepo][more]
KAG6581223.15.0e-6758.33Agamous-like MADS-box protein AGL82, partial [Cucurbita argyrosperma subsp. soro... [more]
Match NameE-valueIdentityDescription
AT5G58890.12.8e-1532.16AGAMOUS-like 82 [more]
AT5G55690.12.8e-1533.12MADS-box transcription factor family protein [more]
AT5G26580.12.7e-1062.00AGAMOUS-like-34 [more]
AT5G48670.14.6e-1042.50AGAMOUS-like 80 [more]
AT5G27960.11.7e-0928.18AGAMOUS-like 90 [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 140..167
NoneNo IPR availablePANTHERPTHR11945MADS BOX PROTEINcoord: 3..186
NoneNo IPR availablePANTHERPTHR11945:SF176AGAMOUS-LIKE MADS-BOX PROTEIN AGL82coord: 3..186
NoneNo IPR availableCDDcd00120MADScoord: 2..50
e-value: 9.11296E-23
score: 86.0285
IPR002100Transcription factor, MADS-boxPRINTSPR00404MADSDOMAINcoord: 3..23
score: 41.02
coord: 23..38
score: 69.15
coord: 38..59
score: 18.18
IPR002100Transcription factor, MADS-boxSMARTSM00432madsneu2coord: 1..60
e-value: 3.1E-16
score: 69.9
IPR002100Transcription factor, MADS-boxPFAMPF00319SRF-TFcoord: 11..53
e-value: 3.4E-17
score: 61.7
IPR002100Transcription factor, MADS-boxPROSITEPS50066MADS_BOX_2coord: 1..49
score: 18.810555
IPR036879Transcription factor, MADS-box superfamilyGENE3D3.40.1810.10coord: 13..104
e-value: 2.6E-18
score: 67.9
IPR036879Transcription factor, MADS-box superfamilySUPERFAMILY55455SRF-likecoord: 2..91

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi05G003610.1Lsi05G003610.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006357 regulation of transcription by RNA polymerase II
molecular_function GO:0000987 cis-regulatory region sequence-specific DNA binding
molecular_function GO:0046983 protein dimerization activity
molecular_function GO:0003677 DNA binding