Lsi05G001390 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi05G001390
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description3-hydroxybutyryl-CoA dehydrogenase
Locationchr05: 2055082 .. 2057813 (+)
RNA-Seq ExpressionLsi05G001390
SyntenyLsi05G001390
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGACGGAAATCAAGGCCATTGGAGTCGTCGGAGGCGGTCAAATGGGATCCGGAATTGCCCAACTCGCCGCCACACACGGCCATGAAGTTTGGCTAATTGATTCAGACCCTGCCGCTCTTGCTAAAGCATCCGACTCCATTTCTTCCTCAATCCAACGCCTGGTCTCTAAAAACCTTCTCTCTCGAGTAAGAATTCTTTCCATTCATTGCTTCGCTTAACTTCTACGTAGTTCTTTTGACTGGAGGGCTTTGAGTGTCATTGGATCCTTTATTAGCTGTTTTATGTTACCTTTGAGTTATCATTTATTGGGTGTTTTGTGTTGTTTATTTTGAAATTGCTCTTAACAGATCAATTGCAGAGAATCACAAAGTGTTAGGGCTTGTTATGGAGTGATTTGGAAATGTCTAAATCTGAAACGTGTATTTCATTATTCAAAATCAATTTATTGTTTGATTTCACACTTTTAAGCATGAGTTTATACCATTGAAATTGATTTTGAATTATTAAAGAGATTTTTCGGAGTTATTTTAAAAATGACAAGCACCCTCTTAATGATTAGGGTGGAAAATGCTTGATTTATTATTATTTATTTGGTTGTTTACTTATGAACAATCTATAGCAATAGGAAATTGCAGTCTCTTGGCCCTATCGTTACCCTTTCTACTGAGGATAACACTGATCCACTGTCTTAGGAAAAAGAAAACAAGAGAATCCTTTCCATATTCCATGTCTCTGCAGAGAAGCTTGTGATATTCTTGTGCTCTCCCATACTGATGGACTAGGAAAATATGGTCTAAAATATTTCAATCCTGCCATGACGACGGTACGTGTCGATGCCAGTGTCAGTACCAAAGATGACAAATATTAGTGGGAGCTTGGCTAAATCCTCTTGAATATAGATCAATAATTGAAATTGAAAATAGTTTCATTCAATGACATATGATCACCAGGGAATTGGATTTGATTGGAGGTAGCCAATGAAACAAGAGTGAACCTTGACGAGCTTCTCTGTGAGCCACAAGATGATGCATTAAACCCAATGTACTTTCTTATAGCTCTTCAATCTTTTGCATTAAACAATAATAACTTGCAGGCTTATAGAAGCCATGAATAATATGGAACTAAATCAATAGAGGAAAGATTTTAGAAGTGGAGAATAAATGGAATGTAAATGAGAATAACTATAGCCAGCTATTGCAATATATTAGAGTGTATTGTAATAATACGATTGTAATAAACAGCTAACGAGTGTTTTGACTGTAGATTCTACCCATGGTGGCTACCTATTTCATAAAGGTTAGGTGACTATGAATAATGCTGAGTAAGGGAGGATTTTTTTTTTTTTTTGTTTTTATCTTTACTAATGTCTTTTCAAATTCTTATGGCCTATGATGCAAAATAAATGTTTCATCCGCATTGAGCACAGTTTGATCCGAAGTTAAAACTGAGTTATTTATTACGGGTTTGTCATTGATTATGGAAAGGCTGACATACAACTCTATAACAGAGTTCTACCGGTCAAATGAACTTCTCTGTTACTCTATTTAAGTTCTGCCGTCCAAATGGTAGATATTTAACCAGCGTATGAAATCCACTTAACAGGAAGTTGGTGTCATTGCTCTTCAACGTCTTAAGTTTTCAACTGATTTGAAAGAGCTTCATTCAGCAGATGTCATCATCGAAGCTATCGTGGAATCAGAAGATGTTAAAAGAAAGCTATTTCTTGAATTAGATAAGATTGCAAAACGGTCTTCCATTCTAGCTTCTAATACAAGTTCCATCCCGATTACTCGCTTAGCATCAGCAACCACACGTCCTGACCAGGTTTCAGCTTCTCTTCCCCCTCATATGCATGAATGCCGATACATAATCATTAATCTTGTGCATTAAACTGCTGTTTTTCTCTTGAAGTGTCTTTGATATGTAGTTTTACTAGCTAGCCATGTGGTTTTAGCATACATTGAATGAAACTACAATTATAAAATTAACATAGGATTTTATCTCAAAATCAATTGGCAATGAGTGGAGTAGTCTCTGTATCAATAAAGATTGTGAGGTCTCTTTGTCTTTCCGATGTGAAGTCTTCAACATTGCAAGCTTGCAAGTGAAACATTTAATATCATTGCAGGTTATTGGCATGCATTTCATGAATCCCCCACCTATAATGAAGTTGGTTGAGATTGTTCGAGGTGCGGATACATCCAATAAAACATTTGATGCCATAAAAATCTTGGCTGAGAGGTGATTCACTTCATTGATTGCTCTCAAATTCGCCTCTTTTCCTCCTCCACCATCAGTTGTCTTTTCTAGTAACACGGGACGACTTCACTTGATAATAGTTTTAACTTTCTTTACTTTCAGGCTTGGCAAAACAGTGATATGTTCTCAGGACTACTCTGGCTTCATAGTGAACAGAATCCTAATGCCAATGATTAACGAGGCGTTTTTTGCTCTCTACACGGGAGTGGCAACGAAGGAAGACATTGACACGGGAATGAAGCTAGGAACAAATCATCCTATGGGTCCCTTGGAGCTCGCAGACTTCATTGGTTTGGATGTCTGTTTATCAATCATGAAGGTTCTCCACTCTGGACTTGGTGACAATAAATATGCTCCCTGCCCTCTTCTCGTGCAGTATGTTGATGCTGGTCGCCTGGGAAGAAAGCGGGGAATAGGCGTATACGATTACCGTAAATTACCGCAATCGGTAAAATCCAAGTCCCGACTTTGA

mRNA sequence

ATGACGGAAATCAAGGCCATTGGAGTCGTCGGAGGCGGTCAAATGGGATCCGGAATTGCCCAACTCGCCGCCACACACGGCCATGAAGTTTGGCTAATTGATTCAGACCCTGCCGCTCTTGCTAAAGCATCCGACTCCATTTCTTCCTCAATCCAACGCCTGGTCTCTAAAAACCTTCTCTCTCGAGAAGTTGGTGTCATTGCTCTTCAACGTCTTAAGTTTTCAACTGATTTGAAAGAGCTTCATTCAGCAGATGTCATCATCGAAGCTATCGTGGAATCAGAAGATGTTAAAAGAAAGCTATTTCTTGAATTAGATAAGATTGCAAAACGGTCTTCCATTCTAGCTTCTAATACAAGTTCCATCCCGATTACTCGCTTAGCATCAGCAACCACACGTCCTGACCAGTTGGTTGAGATTGTTCGAGGTGCGGATACATCCAATAAAACATTTGATGCCATAAAAATCTTGGCTGAGAGGCTTGGCAAAACAGTGATATGTTCTCAGGACTACTCTGGCTTCATAGTGAACAGAATCCTAATGCCAATGATTAACGAGGCGTTTTTTGCTCTCTACACGGGAGTGGCAACGAAGGAAGACATTGACACGGGAATGAAGCTAGGAACAAATCATCCTATGGGTCCCTTGGAGCTCGCAGACTTCATTGGTTTGGATGTCTGTTTATCAATCATGAAGGTTCTCCACTCTGGACTTGGTGACAATAAATATGCTCCCTGCCCTCTTCTCGTGCAGTATGTTGATGCTGGTCGCCTGGGAAGAAAGCGGGGAATAGGCGTATACGATTACCGTAAATTACCGCAATCGGTAAAATCCAAGTCCCGACTTTGA

Coding sequence (CDS)

ATGACGGAAATCAAGGCCATTGGAGTCGTCGGAGGCGGTCAAATGGGATCCGGAATTGCCCAACTCGCCGCCACACACGGCCATGAAGTTTGGCTAATTGATTCAGACCCTGCCGCTCTTGCTAAAGCATCCGACTCCATTTCTTCCTCAATCCAACGCCTGGTCTCTAAAAACCTTCTCTCTCGAGAAGTTGGTGTCATTGCTCTTCAACGTCTTAAGTTTTCAACTGATTTGAAAGAGCTTCATTCAGCAGATGTCATCATCGAAGCTATCGTGGAATCAGAAGATGTTAAAAGAAAGCTATTTCTTGAATTAGATAAGATTGCAAAACGGTCTTCCATTCTAGCTTCTAATACAAGTTCCATCCCGATTACTCGCTTAGCATCAGCAACCACACGTCCTGACCAGTTGGTTGAGATTGTTCGAGGTGCGGATACATCCAATAAAACATTTGATGCCATAAAAATCTTGGCTGAGAGGCTTGGCAAAACAGTGATATGTTCTCAGGACTACTCTGGCTTCATAGTGAACAGAATCCTAATGCCAATGATTAACGAGGCGTTTTTTGCTCTCTACACGGGAGTGGCAACGAAGGAAGACATTGACACGGGAATGAAGCTAGGAACAAATCATCCTATGGGTCCCTTGGAGCTCGCAGACTTCATTGGTTTGGATGTCTGTTTATCAATCATGAAGGTTCTCCACTCTGGACTTGGTGACAATAAATATGCTCCCTGCCCTCTTCTCGTGCAGTATGTTGATGCTGGTCGCCTGGGAAGAAAGCGGGGAATAGGCGTATACGATTACCGTAAATTACCGCAATCGGTAAAATCCAAGTCCCGACTTTGA

Protein sequence

MTEIKAIGVVGGGQMGSGIAQLAATHGHEVWLIDSDPAALAKASDSISSSIQRLVSKNLLSREVGVIALQRLKFSTDLKELHSADVIIEAIVESEDVKRKLFLELDKIAKRSSILASNTSSIPITRLASATTRPDQLVEIVRGADTSNKTFDAIKILAERLGKTVICSQDYSGFIVNRILMPMINEAFFALYTGVATKEDIDTGMKLGTNHPMGPLELADFIGLDVCLSIMKVLHSGLGDNKYAPCPLLVQYVDAGRLGRKRGIGVYDYRKLPQSVKSKSRL
Homology
[Close]
BLAST of Lsi05G001390 vs. ExPASy Swiss-Prot
Match: Q45223 (3-hydroxybutyryl-CoA dehydrogenase OS=Bradyrhizobium diazoefficiens (strain JCM 10833 / BCRC 13528 / IAM 13628 / NBRC 14792 / USDA 110) OX=224911 GN=hbdA PE=3 SV=1)

HSP 1 Score: 270.4 bits (690), Expect = 2.3e-71
Identity = 147/281 (52.31%), Postives = 187/281 (66.55%), Query Frame = 0 

Query: 4   IKAIGVVGGGQMGSGIAQLAATHGHEVWLIDSDPAALAKASDSISSSIQRLVSKNLLSRE 63
           IK +GV+G GQMG+GIA +AA  G +V L D     L     +I+ ++ R VSK +++ E
Sbjct: 5   IKKVGVIGAGQMGNGIAHVAALAGFDVVLNDVSADRLKSGMATINGNLARQVSKKVVTEE 64

Query: 64  VGVIALQRLKFSTDLKELHSADVIIEAIVESEDVKRKLFLELDKIAKRSSILASNTSSIP 123
               AL R+  +  L +L   D++IE  VE E+VKRK+F EL  + K  +I+AS+TSSI 
Sbjct: 65  AKTKALSRIVAAEKLDDLADCDLVIETAVEKEEVKRKIFHELCAVLKPEAIVASDTSSIS 124

Query: 124 ITRLASATTRPD--------------QLVEIVRGADTSNKTFDAIKILAERLGKTVICSQ 183
           ITRLA+AT RP+              +LVE++RG  T + TF+A K    +LGK V  S+
Sbjct: 125 ITRLAAATDRPERFIGIHFMNPVPLMELVELIRGIATDDATFEASKEFVAKLGKQVAVSE 184

Query: 184 DYSGFIVNRILMPMINEAFFALYTGVATKEDIDTGMKLGTNHPMGPLELADFIGLDVCLS 243
           D+  FIVNRIL+PMINEA + LY GV   E ID  MKLG +HPMGPLELADFIGLD CLS
Sbjct: 185 DFPAFIVNRILLPMINEAIYTLYEGVGNVEAIDAAMKLGAHHPMGPLELADFIGLDTCLS 244

Query: 244 IMKVLHSGLGDNKYAPCPLLVQYVDAGRLGRKRGIGVYDYR 271
           IM+VLH GL D+KY PCPLLV+YV+AG LGRK   G YDYR
Sbjct: 245 IMQVLHEGLADSKYRPCPLLVKYVEAGWLGRKTQRGFYDYR 285

[Close]
BLAST of Lsi05G001390 vs. ExPASy Swiss-Prot
Match: P45856 (Probable 3-hydroxybutyryl-CoA dehydrogenase OS=Bacillus subtilis (strain 168) OX=224308 GN=mmgB PE=2 SV=1)

HSP 1 Score: 252.3 bits (643), Expect = 6.5e-66
Identity = 139/283 (49.12%), Postives = 176/283 (62.19%), Query Frame = 0 

Query: 3   EIKAIGVVGGGQMGSGIAQLAATHGHEVWLIDSDPAALAKASDSISSSIQRLVSKNLLSR 62
           EIK I V G GQMGSGIAQ AA  G  V + D +P A       +   + R   K   + 
Sbjct: 2   EIKQIMVAGAGQMGSGIAQTAADAGFYVRMYDVNPEAAEAGLKRLKKQLARDAEKGKRTE 61

Query: 63  EVGVIALQRLKFSTDLKELHSADVIIEAIVESEDVKRKLFLELDKIAKRSSILASNTSSI 122
                 + R+  S  L+E   AD++IEAI E+   K ++F  LD+I    +ILASNTSS+
Sbjct: 62  TEVKSVINRISISQTLEEAEHADIVIEAIAENMAAKTEMFKTLDRICPPHTILASNTSSL 121

Query: 123 PITRLASATTRPD--------------QLVEIVRGADTSNKTFDAIKILAERLGKTVICS 182
           PIT +A+ T RP               +LVE++RG  TS +T   +  LAE++GKT +  
Sbjct: 122 PITEIAAVTNRPQRVIGMHFMNPVPVMKLVEVIRGLATSEETALDVMALAEKMGKTAVEV 181

Query: 183 QDYSGFIVNRILMPMINEAFFALYTGVATKEDIDTGMKLGTNHPMGPLELADFIGLDVCL 242
            D+ GF+ NR+L+PMINEA + +Y GVA  E ID  MKLG NHPMGPL LADFIGLD CL
Sbjct: 182 NDFPGFVSNRVLLPMINEAIYCVYEGVAKPEAIDEVMKLGMNHPMGPLALADFIGLDTCL 241

Query: 243 SIMKVLHSGLGDNKYAPCPLLVQYVDAGRLGRKRGIGVYDYRK 272
           SIM+VLHSGLGD+KY PCPLL +YV AG LG+K G G YDY +
Sbjct: 242 SIMEVLHSGLGDSKYRPCPLLRKYVKAGWLGKKSGRGFYDYEE 284

[Close]
BLAST of Lsi05G001390 vs. ExPASy Swiss-Prot
Match: P52041 (3-hydroxybutyryl-CoA dehydrogenase OS=Clostridium acetobutylicum (strain ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787) OX=272562 GN=hbd PE=1 SV=2)

HSP 1 Score: 245.4 bits (625), Expect = 7.9e-64
Identity = 138/282 (48.94%), Postives = 178/282 (63.12%), Query Frame = 0 

Query: 4   IKAIGVVGGGQMGSGIAQLAATHGHEVWLIDSDPAALAKASDSISSSIQRLVSKNLLSRE 63
           +K + V+G G MGSGIAQ  A  G EV L D     + +  D I+ ++ +LV K  +   
Sbjct: 1   MKKVCVIGAGTMGSGIAQAFAAKGFEVVLRDIKDEFVDRGLDFINKNLSKLVKKGKIEEA 60

Query: 64  VGVIALQRLKFSTDLKELHSADVIIEAIVESEDVKRKLFLELDKIAKRSSILASNTSSIP 123
             V  L R+  + DL      D++IEA VE  D+K+++F +LD I K  +ILASNTSS+ 
Sbjct: 61  TKVEILTRISGTVDLNMAADCDLVIEAAVERMDIKKQIFADLDNICKPETILASNTSSLS 120

Query: 124 ITRLASATTRPD--------------QLVEIVRGADTSNKTFDAIKILAERLGKTVICSQ 183
           IT +ASAT RPD              +LVE++RG  TS +TFDA+K  +  +GK  +   
Sbjct: 121 ITEVASATKRPDKVIGMHFFNPAPVMKLVEVIRGIATSQETFDAVKETSIAIGKDPVEVA 180

Query: 184 DYSGFIVNRILMPMINEAFFALYTGVATKEDIDTGMKLGTNHPMGPLELADFIGLDVCLS 243
           +  GF+VNRIL+PMINEA   L  G+A+ EDID  MKLG NHPMGPLEL DFIGLD+CL+
Sbjct: 181 EAPGFVVNRILIPMINEAVGILAEGIASVEDIDKAMKLGANHPMGPLELGDFIGLDICLA 240

Query: 244 IMKVLHSGLGDNKYAPCPLLVQYVDAGRLGRKRGIGVYDYRK 272
           IM VL+S  GD+KY P  LL +YV AG LGRK G G YDY K
Sbjct: 241 IMDVLYSETGDSKYRPHTLLKKYVRAGWLGRKSGKGFYDYSK 282

[Close]
BLAST of Lsi05G001390 vs. ExPASy Swiss-Prot
Match: Q9RVG1 (Probable 3-hydroxybutyryl-CoA dehydrogenase OS=Deinococcus radiodurans (strain ATCC 13939 / DSM 20539 / JCM 16871 / LMG 4051 / NBRC 15346 / NCIMB 9279 / R1 / VKM B-1422) OX=243230 GN=hbd PE=3 SV=1)

HSP 1 Score: 244.6 bits (623), Expect = 1.4e-63
Identity = 138/276 (50.00%), Postives = 176/276 (63.77%), Query Frame = 0 

Query: 8   GVVGGGQMGSGIAQLAATHGHEVWLIDSDPAALAKASDSISSSIQRLVSKNLLSREVGVI 67
           GV+G GQMG GIAQ+AA  G +V + D     L +    I  S+ +L  K  L+ +    
Sbjct: 4   GVIGAGQMGGGIAQVAAQSGFDVVVHDQKQEFLDRGKGVIEKSLGKLHEKGKLT-DAPET 63

Query: 68  ALQRLKFSTDLKELHSADVIIEAIVESEDVKRKLFLELDKIAKRSSILASNTSSIPITRL 127
            L R+ F+TDL++    D+++EAIVE++ +K  LF +L +I K   ILASNTSSIPIT L
Sbjct: 64  VLGRIHFTTDLQDFADCDLVVEAIVENQQIKNDLFKQLGQIVKPEGILASNTSSIPITAL 123

Query: 128 ASATTRPD--------------QLVEIVRGADTSNKTFDAIKILAERLGKTVICSQDYSG 187
           A+A+ RP               QLVE++RG  TS++T   +   AE+LGKT +   D+ G
Sbjct: 124 ATASGRPAQFIGMHFMNPVPLMQLVEVIRGYQTSDETARIVTETAEKLGKTPLSCNDFPG 183

Query: 188 FIVNRILMPMINEAFFALYTGVATKEDIDTGMKLGTNHPMGPLELADFIGLDVCLSIMKV 247
           F+ NRILMPM+NEA   +  GVA  E ID  MKLG NHPMGPL LADFIGLD CL+IM+V
Sbjct: 184 FVSNRILMPMLNEAIQCVMEGVAEPEAIDGIMKLGMNHPMGPLTLADFIGLDTCLAIMEV 243

Query: 248 LHSGLGDNKYAPCPLLVQYVDAGRLGRKRGIGVYDY 270
           LH GLGD+KY P PLL + V AG LGRK G G Y Y
Sbjct: 244 LHQGLGDDKYRPSPLLRKMVQAGLLGRKSGEGFYKY 278

[Close]
BLAST of Lsi05G001390 vs. ExPASy Swiss-Prot
Match: P77851 (3-hydroxybutyryl-CoA dehydrogenase OS=Thermoanaerobacterium thermosaccharolyticum (strain ATCC 7956 / DSM 571 / NCIMB 9385 / NCA 3814 / NCTC 13789 / WDCM 00135 / 2032) OX=580327 GN=hbd PE=3 SV=2)

HSP 1 Score: 233.0 bits (593), Expect = 4.1e-60
Identity = 130/281 (46.26%), Postives = 184/281 (65.48%), Query Frame = 0 

Query: 4   IKAIGVVGGGQMGSGIAQLAATHGHEVWLIDSDPAALAKASDSISSSIQRLVSKNLLSRE 63
           ++ I V+G G MGSGIAQ+ A +G EV L D D   + K   +I  ++QR V K  ++ +
Sbjct: 1   MQKICVIGAGTMGSGIAQVFAQNGFEVILRDIDMKFVEKGFGTIEKNLQRNVDKGKITAD 60

Query: 64  VGVIALQRLKFSTDLKELHSADVIIEAIVESEDVKRKLFLELDKIAKRSSILASNTSSIP 123
                L R++ +T+L++   AD ++EA +E+ D+K+++F ELD+I K  +ILASNTSS+ 
Sbjct: 61  EKNEILSRIRGTTNLEDAKEADFVVEAAIENMDLKKQIFKELDEICKMETILASNTSSLS 120

Query: 124 ITRLASATTRPD--------------QLVEIVRGADTSNKTFDAIKILAERLGKTVICSQ 183
           IT +ASAT RP+              +LVE+++G  TS +TF+ ++ LA ++ KT I  +
Sbjct: 121 ITEIASATKRPEKVIGMHFFNPVPVMKLVEVIKGLKTSEQTFNVVRELALKVDKTPIEVK 180

Query: 184 DYSGFIVNRILMPMINEAFFALYTGVATKEDIDTGMKLGTNHPMGPLELADFIGLDVCLS 243
           +  GF+VNRIL+PMINEA   L  GVAT EDID  MKLG NHP+GPL L+D IG DV L+
Sbjct: 181 EAPGFVVNRILIPMINEAIGILADGVATAEDIDEAMKLGANHPIGPLALSDLIGNDVVLA 240

Query: 244 IMKVLHSGLGDNKYAPCPLLVQYVDAGRLGRKRGIGVYDYR 271
           IM VL+   GD+KY P PLL + V  G LGRK G G ++Y+
Sbjct: 241 IMNVLYEEYGDSKYRPHPLLKKMVRGGLLGRKTGKGFFEYK 281

[Close]
BLAST of Lsi05G001390 vs. ExPASy TrEMBL
Match: A0A5D3CH80 (3-hydroxybutyryl-CoA dehydrogenase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold227G00980 PE=3 SV=1)

HSP 1 Score: 509.6 bits (1311), Expect = 8.3e-141
Identity = 269/296 (90.88%), Postives = 276/296 (93.24%), Query Frame = 0 

Query: 1   MTEIKAIGVVGGGQMGSGIAQLAATHGHEVWLIDSDPAALAKASDSISSSIQRLVSKNLL 60
           MTEIKAIGVVGGGQMGSGIAQLAATHGH+VWLIDSDPAALAKA+DSISSSIQRLVS+NLL
Sbjct: 1   MTEIKAIGVVGGGQMGSGIAQLAATHGHQVWLIDSDPAALAKATDSISSSIQRLVSRNLL 60

Query: 61  SREVGVIALQRLKFSTDLKELHSADVIIEAIVESEDVKRKLFLELDKIAKRSSILASNTS 120
           SRE+G IALQRLKFSTDLKELHSADVIIEAIVESEDVKRKLF+ELDKIAKRSSILASNTS
Sbjct: 61  SREIGAIALQRLKFSTDLKELHSADVIIEAIVESEDVKRKLFVELDKIAKRSSILASNTS 120

Query: 121 SIPITRLASATTRPDQ--------------LVEIVRGADTSNKTFDAIKILAERLGKTVI 180
           SIPITRLASATTRP+Q              LVEIVRGADTSN+TFDAIKILAERLGKTVI
Sbjct: 121 SIPITRLASATTRPEQVIGMHFMNPPPIMKLVEIVRGADTSNETFDAIKILAERLGKTVI 180

Query: 181 CSQDYSGFIVNRILMPMINEAFFALYTGVATKEDIDTGMKLGTNHPMGPLELADFIGLDV 240
           CSQDYSGFIVNRILMPMINEAFF LYTGVATKEDIDTGMKLGTNHPMGPLELADFIGLDV
Sbjct: 181 CSQDYSGFIVNRILMPMINEAFFTLYTGVATKEDIDTGMKLGTNHPMGPLELADFIGLDV 240

Query: 241 CLSIMKVLHSGLGDNKYAPCPLLVQYVDAGRLGRKRGIGVYDYRKLPQSVKSKSRL 283
           CLSIMKVLHSGLGDNKYAPCPLLVQYVDAGRLGRKRGIGVYDYRK P   KSKSRL
Sbjct: 241 CLSIMKVLHSGLGDNKYAPCPLLVQYVDAGRLGRKRGIGVYDYRKQPLLGKSKSRL 296

[Close]
BLAST of Lsi05G001390 vs. ExPASy TrEMBL
Match: A0A5A7USC9 (3-hydroxybutyryl-CoA dehydrogenase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold803G00200 PE=3 SV=1)

HSP 1 Score: 506.1 bits (1302), Expect = 9.2e-140
Identity = 267/296 (90.20%), Postives = 275/296 (92.91%), Query Frame = 0 

Query: 1   MTEIKAIGVVGGGQMGSGIAQLAATHGHEVWLIDSDPAALAKASDSISSSIQRLVSKNLL 60
           MTEIKAIGVVGGGQMGSGIAQLAATHGH+VWLIDSDPAALAKA+DSISSSIQRLVS+NLL
Sbjct: 14  MTEIKAIGVVGGGQMGSGIAQLAATHGHQVWLIDSDPAALAKATDSISSSIQRLVSRNLL 73

Query: 61  SREVGVIALQRLKFSTDLKELHSADVIIEAIVESEDVKRKLFLELDKIAKRSSILASNTS 120
           SRE+G IALQRLKFSTDLKELHSADVIIEAIVESEDVKRKLF+ELDKIAKRSSILASNTS
Sbjct: 74  SREIGAIALQRLKFSTDLKELHSADVIIEAIVESEDVKRKLFVELDKIAKRSSILASNTS 133

Query: 121 SIPITRLASATTRPDQ--------------LVEIVRGADTSNKTFDAIKILAERLGKTVI 180
           SIPITRLASATTRP+Q              LVEIVRGADTS++TFDAIKILAERLGKTVI
Sbjct: 134 SIPITRLASATTRPEQVIGMHFMNPPPIMKLVEIVRGADTSDETFDAIKILAERLGKTVI 193

Query: 181 CSQDYSGFIVNRILMPMINEAFFALYTGVATKEDIDTGMKLGTNHPMGPLELADFIGLDV 240
           CSQDYSGFIVNRILMPMINEAFF LYTGVATKEDIDTGMKLGTNHPMGPLELADFIGLDV
Sbjct: 194 CSQDYSGFIVNRILMPMINEAFFTLYTGVATKEDIDTGMKLGTNHPMGPLELADFIGLDV 253

Query: 241 CLSIMKVLHSGLGDNKYAPCPLLVQYVDAGRLGRKRGIGVYDYRKLPQSVKSKSRL 283
           CLSIMKVLHSGLGDNKYAPCPLLVQY DAGRLGRKRGIGVYDYRK P   KSKSRL
Sbjct: 254 CLSIMKVLHSGLGDNKYAPCPLLVQYADAGRLGRKRGIGVYDYRKQPLLGKSKSRL 309

[Close]
BLAST of Lsi05G001390 vs. ExPASy TrEMBL
Match: A0A1S3C2Y7 (3-hydroxybutyryl-CoA dehydrogenase OS=Cucumis melo OX=3656 GN=LOC103496074 PE=3 SV=1)

HSP 1 Score: 506.1 bits (1302), Expect = 9.2e-140
Identity = 267/296 (90.20%), Postives = 275/296 (92.91%), Query Frame = 0 

Query: 1   MTEIKAIGVVGGGQMGSGIAQLAATHGHEVWLIDSDPAALAKASDSISSSIQRLVSKNLL 60
           MTEIKAIGVVGGGQMGSGIAQLAATHGH+VWLIDSDPAALAKA+DSISSSIQRLVS+NLL
Sbjct: 14  MTEIKAIGVVGGGQMGSGIAQLAATHGHQVWLIDSDPAALAKATDSISSSIQRLVSRNLL 73

Query: 61  SREVGVIALQRLKFSTDLKELHSADVIIEAIVESEDVKRKLFLELDKIAKRSSILASNTS 120
           SRE+G IALQRLKFSTDLKELHSADVIIEAIVESEDVKRKLF+ELDKIAKRSSILASNTS
Sbjct: 74  SREIGAIALQRLKFSTDLKELHSADVIIEAIVESEDVKRKLFVELDKIAKRSSILASNTS 133

Query: 121 SIPITRLASATTRPDQ--------------LVEIVRGADTSNKTFDAIKILAERLGKTVI 180
           SIPITRLASATTRP+Q              LVEIVRGADTS++TFDAIKILAERLGKTVI
Sbjct: 134 SIPITRLASATTRPEQVIGMHFMNPPPIMKLVEIVRGADTSDETFDAIKILAERLGKTVI 193

Query: 181 CSQDYSGFIVNRILMPMINEAFFALYTGVATKEDIDTGMKLGTNHPMGPLELADFIGLDV 240
           CSQDYSGFIVNRILMPMINEAFF LYTGVATKEDIDTGMKLGTNHPMGPLELADFIGLDV
Sbjct: 194 CSQDYSGFIVNRILMPMINEAFFTLYTGVATKEDIDTGMKLGTNHPMGPLELADFIGLDV 253

Query: 241 CLSIMKVLHSGLGDNKYAPCPLLVQYVDAGRLGRKRGIGVYDYRKLPQSVKSKSRL 283
           CLSIMKVLHSGLGDNKYAPCPLLVQY DAGRLGRKRGIGVYDYRK P   KSKSRL
Sbjct: 254 CLSIMKVLHSGLGDNKYAPCPLLVQYADAGRLGRKRGIGVYDYRKQPLLGKSKSRL 309

[Close]
BLAST of Lsi05G001390 vs. ExPASy TrEMBL
Match: A0A0A0L6N5 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G234000 PE=3 SV=1)

HSP 1 Score: 503.1 bits (1294), Expect = 7.8e-139
Identity = 266/296 (89.86%), Postives = 276/296 (93.24%), Query Frame = 0 

Query: 1   MTEIKAIGVVGGGQMGSGIAQLAATHGHEVWLIDSDPAALAKASDSISSSIQRLVSKNLL 60
           MTEIKAIGVVGGGQMGSGIAQLAAT+GH+VWLIDSD AALAKA+DSISSSIQRLVS+NLL
Sbjct: 1   MTEIKAIGVVGGGQMGSGIAQLAATYGHQVWLIDSDSAALAKATDSISSSIQRLVSRNLL 60

Query: 61  SREVGVIALQRLKFSTDLKELHSADVIIEAIVESEDVKRKLFLELDKIAKRSSILASNTS 120
           SRE+G  ALQRLKFSTDLKELHSADVIIEAI+ESEDVKRKLF+ELDKIAKRSSILASNTS
Sbjct: 61  SREIGANALQRLKFSTDLKELHSADVIIEAILESEDVKRKLFVELDKIAKRSSILASNTS 120

Query: 121 SIPITRLASATTRPDQ--------------LVEIVRGADTSNKTFDAIKILAERLGKTVI 180
           SIPITRLASATTRP+Q              LVEIVRGADTSN+TFDAIKILAERLGKTVI
Sbjct: 121 SIPITRLASATTRPEQVIGMHFMNPPPVMKLVEIVRGADTSNETFDAIKILAERLGKTVI 180

Query: 181 CSQDYSGFIVNRILMPMINEAFFALYTGVATKEDIDTGMKLGTNHPMGPLELADFIGLDV 240
           CSQDYSGFIVNRILMPMINEAFFALYTGVATKEDIDTGMKLGTNHPMGPLELADFIGLDV
Sbjct: 181 CSQDYSGFIVNRILMPMINEAFFALYTGVATKEDIDTGMKLGTNHPMGPLELADFIGLDV 240

Query: 241 CLSIMKVLHSGLGDNKYAPCPLLVQYVDAGRLGRKRGIGVYDYRKLPQSVKSKSRL 283
           CLSIMKVLHSGLGDNKYAPCPLLVQYVDAGRLGRKR IGVYDYRKLP+  KSKSRL
Sbjct: 241 CLSIMKVLHSGLGDNKYAPCPLLVQYVDAGRLGRKRRIGVYDYRKLPEPDKSKSRL 296

[Close]
BLAST of Lsi05G001390 vs. ExPASy TrEMBL
Match: A0A6J1CM78 (uncharacterized protein LOC111012899 OS=Momordica charantia OX=3673 GN=LOC111012899 PE=3 SV=1)

HSP 1 Score: 483.4 bits (1243), Expect = 6.4e-133
Identity = 256/294 (87.07%), Postives = 267/294 (90.82%), Query Frame = 0 

Query: 3   EIKAIGVVGGGQMGSGIAQLAATHGHEVWLIDSDPAALAKASDSISSSIQRLVSKNLLSR 62
           EI+ IGVVGGGQMGSGIAQLAATHG +VWLIDSDPAALAKA++SIS+SIQRLVSKNLLS+
Sbjct: 2   EIRTIGVVGGGQMGSGIAQLAATHGCQVWLIDSDPAALAKANESISASIQRLVSKNLLSQ 61

Query: 63  EVGVIALQRLKFSTDLKELHSADVIIEAIVESEDVKRKLFLELDKIAKRSSILASNTSSI 122
           EV V ALQRLKFSTDLKELHSADV+IEAIVESEDVKR+LFLELDKI KRS ILASNTSSI
Sbjct: 62  EVSVSALQRLKFSTDLKELHSADVVIEAIVESEDVKRRLFLELDKIVKRSGILASNTSSI 121

Query: 123 PITRLASATTRPDQ--------------LVEIVRGADTSNKTFDAIKILAERLGKTVICS 182
           PITRLASATTRP Q              LVEIVRGADTSN+TFDAIK LAERLGKTVICS
Sbjct: 122 PITRLASATTRPKQVIGMHFMNPPPIMKLVEIVRGADTSNETFDAIKTLAERLGKTVICS 181

Query: 183 QDYSGFIVNRILMPMINEAFFALYTGVATKEDIDTGMKLGTNHPMGPLELADFIGLDVCL 242
           QDYSGFIVNRIL+PMINEAFFALYTGVATKEDIDTGMKLGTNHPMGPLELADFIGLDVCL
Sbjct: 182 QDYSGFIVNRILIPMINEAFFALYTGVATKEDIDTGMKLGTNHPMGPLELADFIGLDVCL 241

Query: 243 SIMKVLHSGLGDNKYAPCPLLVQYVDAGRLGRKRGIGVYDYRKLPQSVKSKSRL 283
           SIMKVLHSGLGD KYAPCPLLVQYVDAGRLGRKRGIGVYDYRK+ Q  KS SRL
Sbjct: 242 SIMKVLHSGLGDGKYAPCPLLVQYVDAGRLGRKRGIGVYDYRKVTQPKKSTSRL 295

[Close]
BLAST of Lsi05G001390 vs. NCBI nr
Match: TYK11243.1 (3-hydroxybutyryl-CoA dehydrogenase [Cucumis melo var. makuwa])

HSP 1 Score: 509.6 bits (1311), Expect = 1.7e-140
Identity = 269/296 (90.88%), Postives = 276/296 (93.24%), Query Frame = 0 

Query: 1   MTEIKAIGVVGGGQMGSGIAQLAATHGHEVWLIDSDPAALAKASDSISSSIQRLVSKNLL 60
           MTEIKAIGVVGGGQMGSGIAQLAATHGH+VWLIDSDPAALAKA+DSISSSIQRLVS+NLL
Sbjct: 1   MTEIKAIGVVGGGQMGSGIAQLAATHGHQVWLIDSDPAALAKATDSISSSIQRLVSRNLL 60

Query: 61  SREVGVIALQRLKFSTDLKELHSADVIIEAIVESEDVKRKLFLELDKIAKRSSILASNTS 120
           SRE+G IALQRLKFSTDLKELHSADVIIEAIVESEDVKRKLF+ELDKIAKRSSILASNTS
Sbjct: 61  SREIGAIALQRLKFSTDLKELHSADVIIEAIVESEDVKRKLFVELDKIAKRSSILASNTS 120

Query: 121 SIPITRLASATTRPDQ--------------LVEIVRGADTSNKTFDAIKILAERLGKTVI 180
           SIPITRLASATTRP+Q              LVEIVRGADTSN+TFDAIKILAERLGKTVI
Sbjct: 121 SIPITRLASATTRPEQVIGMHFMNPPPIMKLVEIVRGADTSNETFDAIKILAERLGKTVI 180

Query: 181 CSQDYSGFIVNRILMPMINEAFFALYTGVATKEDIDTGMKLGTNHPMGPLELADFIGLDV 240
           CSQDYSGFIVNRILMPMINEAFF LYTGVATKEDIDTGMKLGTNHPMGPLELADFIGLDV
Sbjct: 181 CSQDYSGFIVNRILMPMINEAFFTLYTGVATKEDIDTGMKLGTNHPMGPLELADFIGLDV 240

Query: 241 CLSIMKVLHSGLGDNKYAPCPLLVQYVDAGRLGRKRGIGVYDYRKLPQSVKSKSRL 283
           CLSIMKVLHSGLGDNKYAPCPLLVQYVDAGRLGRKRGIGVYDYRK P   KSKSRL
Sbjct: 241 CLSIMKVLHSGLGDNKYAPCPLLVQYVDAGRLGRKRGIGVYDYRKQPLLGKSKSRL 296

[Close]
BLAST of Lsi05G001390 vs. NCBI nr
Match: XP_038878166.1 (3-hydroxybutyryl-CoA dehydrogenase [Benincasa hispida])

HSP 1 Score: 509.6 bits (1311), Expect = 1.7e-140
Identity = 270/296 (91.22%), Postives = 276/296 (93.24%), Query Frame = 0 

Query: 1   MTEIKAIGVVGGGQMGSGIAQLAATHGHEVWLIDSDPAALAKASDSISSSIQRLVSKNLL 60
           MTEIKAIGVVGGGQMGSGIAQLAATHGHEVWLIDSDPAALAKASDSISSSIQRL+SKNLL
Sbjct: 1   MTEIKAIGVVGGGQMGSGIAQLAATHGHEVWLIDSDPAALAKASDSISSSIQRLISKNLL 60

Query: 61  SREVGVIALQRLKFSTDLKELHSADVIIEAIVESEDVKRKLFLELDKIAKRSSILASNTS 120
           SREVGV ALQRLKFSTDLKELHSADVIIEAIVE EDVKRKLFLELDKIAKRSSILASNTS
Sbjct: 61  SREVGVNALQRLKFSTDLKELHSADVIIEAIVELEDVKRKLFLELDKIAKRSSILASNTS 120

Query: 121 SIPITRLASATTRPDQ--------------LVEIVRGADTSNKTFDAIKILAERLGKTVI 180
           SIPITRLASATTRP+Q              LVEI+RGADTS++TFDAIKILAERLGKTVI
Sbjct: 121 SIPITRLASATTRPEQVIGMHFMNPPPIMKLVEIIRGADTSDETFDAIKILAERLGKTVI 180

Query: 181 CSQDYSGFIVNRILMPMINEAFFALYTGVATKEDIDTGMKLGTNHPMGPLELADFIGLDV 240
           CSQDYSGFIVNRILMPMINEAFF LYTGVATKEDID GMKLGTN+PMGPLELADFIGLDV
Sbjct: 181 CSQDYSGFIVNRILMPMINEAFFTLYTGVATKEDIDKGMKLGTNYPMGPLELADFIGLDV 240

Query: 241 CLSIMKVLHSGLGDNKYAPCPLLVQYVDAGRLGRKRGIGVYDYRKLPQSVKSKSRL 283
           CLSIMKVLH GLGDNKYAPCPLLVQYVDAGRLGRKRGIGVYDYRKLPQS KSKSRL
Sbjct: 241 CLSIMKVLHCGLGDNKYAPCPLLVQYVDAGRLGRKRGIGVYDYRKLPQSQKSKSRL 296

[Close]
BLAST of Lsi05G001390 vs. NCBI nr
Match: XP_008456022.1 (PREDICTED: 3-hydroxybutyryl-CoA dehydrogenase [Cucumis melo] >KAA0058823.1 3-hydroxybutyryl-CoA dehydrogenase [Cucumis melo var. makuwa])

HSP 1 Score: 506.1 bits (1302), Expect = 1.9e-139
Identity = 267/296 (90.20%), Postives = 275/296 (92.91%), Query Frame = 0 

Query: 1   MTEIKAIGVVGGGQMGSGIAQLAATHGHEVWLIDSDPAALAKASDSISSSIQRLVSKNLL 60
           MTEIKAIGVVGGGQMGSGIAQLAATHGH+VWLIDSDPAALAKA+DSISSSIQRLVS+NLL
Sbjct: 14  MTEIKAIGVVGGGQMGSGIAQLAATHGHQVWLIDSDPAALAKATDSISSSIQRLVSRNLL 73

Query: 61  SREVGVIALQRLKFSTDLKELHSADVIIEAIVESEDVKRKLFLELDKIAKRSSILASNTS 120
           SRE+G IALQRLKFSTDLKELHSADVIIEAIVESEDVKRKLF+ELDKIAKRSSILASNTS
Sbjct: 74  SREIGAIALQRLKFSTDLKELHSADVIIEAIVESEDVKRKLFVELDKIAKRSSILASNTS 133

Query: 121 SIPITRLASATTRPDQ--------------LVEIVRGADTSNKTFDAIKILAERLGKTVI 180
           SIPITRLASATTRP+Q              LVEIVRGADTS++TFDAIKILAERLGKTVI
Sbjct: 134 SIPITRLASATTRPEQVIGMHFMNPPPIMKLVEIVRGADTSDETFDAIKILAERLGKTVI 193

Query: 181 CSQDYSGFIVNRILMPMINEAFFALYTGVATKEDIDTGMKLGTNHPMGPLELADFIGLDV 240
           CSQDYSGFIVNRILMPMINEAFF LYTGVATKEDIDTGMKLGTNHPMGPLELADFIGLDV
Sbjct: 194 CSQDYSGFIVNRILMPMINEAFFTLYTGVATKEDIDTGMKLGTNHPMGPLELADFIGLDV 253

Query: 241 CLSIMKVLHSGLGDNKYAPCPLLVQYVDAGRLGRKRGIGVYDYRKLPQSVKSKSRL 283
           CLSIMKVLHSGLGDNKYAPCPLLVQY DAGRLGRKRGIGVYDYRK P   KSKSRL
Sbjct: 254 CLSIMKVLHSGLGDNKYAPCPLLVQYADAGRLGRKRGIGVYDYRKQPLLGKSKSRL 309

[Close]
BLAST of Lsi05G001390 vs. NCBI nr
Match: XP_004146246.1 (uncharacterized protein LOC101221475 isoform X1 [Cucumis sativus])

HSP 1 Score: 503.1 bits (1294), Expect = 1.6e-138
Identity = 266/296 (89.86%), Postives = 276/296 (93.24%), Query Frame = 0 

Query: 1   MTEIKAIGVVGGGQMGSGIAQLAATHGHEVWLIDSDPAALAKASDSISSSIQRLVSKNLL 60
           MTEIKAIGVVGGGQMGSGIAQLAAT+GH+VWLIDSD AALAKA+DSISSSIQRLVS+NLL
Sbjct: 1   MTEIKAIGVVGGGQMGSGIAQLAATYGHQVWLIDSDSAALAKATDSISSSIQRLVSRNLL 60

Query: 61  SREVGVIALQRLKFSTDLKELHSADVIIEAIVESEDVKRKLFLELDKIAKRSSILASNTS 120
           SRE+G  ALQRLKFSTDLKELHSADVIIEAI+ESEDVKRKLF+ELDKIAKRSSILASNTS
Sbjct: 61  SREIGANALQRLKFSTDLKELHSADVIIEAILESEDVKRKLFVELDKIAKRSSILASNTS 120

Query: 121 SIPITRLASATTRPDQ--------------LVEIVRGADTSNKTFDAIKILAERLGKTVI 180
           SIPITRLASATTRP+Q              LVEIVRGADTSN+TFDAIKILAERLGKTVI
Sbjct: 121 SIPITRLASATTRPEQVIGMHFMNPPPVMKLVEIVRGADTSNETFDAIKILAERLGKTVI 180

Query: 181 CSQDYSGFIVNRILMPMINEAFFALYTGVATKEDIDTGMKLGTNHPMGPLELADFIGLDV 240
           CSQDYSGFIVNRILMPMINEAFFALYTGVATKEDIDTGMKLGTNHPMGPLELADFIGLDV
Sbjct: 181 CSQDYSGFIVNRILMPMINEAFFALYTGVATKEDIDTGMKLGTNHPMGPLELADFIGLDV 240

Query: 241 CLSIMKVLHSGLGDNKYAPCPLLVQYVDAGRLGRKRGIGVYDYRKLPQSVKSKSRL 283
           CLSIMKVLHSGLGDNKYAPCPLLVQYVDAGRLGRKR IGVYDYRKLP+  KSKSRL
Sbjct: 241 CLSIMKVLHSGLGDNKYAPCPLLVQYVDAGRLGRKRRIGVYDYRKLPEPDKSKSRL 296

[Close]
BLAST of Lsi05G001390 vs. NCBI nr
Match: XP_023553026.1 (uncharacterized protein LOC111810545 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 484.6 bits (1246), Expect = 5.9e-133
Identity = 255/296 (86.15%), Postives = 271/296 (91.55%), Query Frame = 0 

Query: 1   MTEIKAIGVVGGGQMGSGIAQLAATHGHEVWLIDSDPAALAKASDSISSSIQRLVSKNLL 60
           MTEIK IGVVGGGQMGSGIAQLAA +GH+VWLIDSDPAAL KA++SISSSIQRLVS+NLL
Sbjct: 1   MTEIKVIGVVGGGQMGSGIAQLAAAYGHDVWLIDSDPAALTKATNSISSSIQRLVSRNLL 60

Query: 61  SREVGVIALQRLKFSTDLKELHSADVIIEAIVESEDVKRKLFLELDKIAKRSSILASNTS 120
           SREVGV ALQRLK STDLKELHSADVIIEAIVESEDVKRKLFLELDKIAK S+ILASNTS
Sbjct: 61  SREVGVSALQRLKCSTDLKELHSADVIIEAIVESEDVKRKLFLELDKIAKPSAILASNTS 120

Query: 121 SIPITRLASATTRPD--------------QLVEIVRGADTSNKTFDAIKILAERLGKTVI 180
           SIPITRLAS+TTRPD              +LVEIVRGADTS++TF AIK LAERLGKT+I
Sbjct: 121 SIPITRLASSTTRPDKVIGMHFMNPPPIMKLVEIVRGADTSDETFHAIKTLAERLGKTLI 180

Query: 181 CSQDYSGFIVNRILMPMINEAFFALYTGVATKEDIDTGMKLGTNHPMGPLELADFIGLDV 240
           CSQDYSGFIVNRILMPMINEAFFALYTGVATKEDIDTGMKLGTNHPMGPLELAD IGLDV
Sbjct: 181 CSQDYSGFIVNRILMPMINEAFFALYTGVATKEDIDTGMKLGTNHPMGPLELADLIGLDV 240

Query: 241 CLSIMKVLHSGLGDNKYAPCPLLVQYVDAGRLGRKRGIGVYDYRKLPQSVKSKSRL 283
           C+SIMKVLHSGLGD+KYAPCPLLVQYVDAGRLGRKRG+G+YDYRKLP+  KSKSRL
Sbjct: 241 CVSIMKVLHSGLGDDKYAPCPLLVQYVDAGRLGRKRGLGIYDYRKLPKPEKSKSRL 296

[Close]
BLAST of Lsi05G001390 vs. TAIR 10
Match: AT3G15290.1 (3-hydroxyacyl-CoA dehydrogenase family protein )

HSP 1 Score: 399.1 bits (1024), Expect = 3.1e-111
Identity = 204/293 (69.62%), Postives = 244/293 (83.28%), Query Frame = 0 

Query: 1   MTEIKAIGVVGGGQMGSGIAQLAATHGHEVWLIDSDPAALAKASDSISSSIQRLVSKNLL 60
           M E+K++GVVG GQMGSGIAQLAAT G +VWL+D+D  AL++A+ +ISSS++R VSK L+
Sbjct: 1   MAEMKSVGVVGAGQMGSGIAQLAATSGLDVWLMDADRDALSRATAAISSSVKRFVSKGLI 60

Query: 61  SREVGVIALQRLKFSTDLKELHSADVIIEAIVESEDVKRKLFLELDKIAKRSSILASNTS 120
           S+EVG  A+ RL+ +++L++L SAD+I+EAIVESED+K+KLF +LD IAK S+ILASNTS
Sbjct: 61  SKEVGDDAMHRLRLTSNLEDLCSADIIVEAIVESEDIKKKLFKDLDGIAKSSAILASNTS 120

Query: 121 SIPITRLASATTRPDQ--------------LVEIVRGADTSNKTFDAIKILAERLGKTVI 180
           SI ITRLASAT RP Q              LVEI+RGADTS +TF A K+LAER GKT +
Sbjct: 121 SISITRLASATRRPSQVIGMHFMNPPPIMKLVEIIRGADTSEETFLATKVLAERFGKTTV 180

Query: 181 CSQDYSGFIVNRILMPMINEAFFALYTGVATKEDIDTGMKLGTNHPMGPLELADFIGLDV 240
           CSQDY+GF+VNRILMPMINEAF  LYTGVATKEDID+GMK GTNHPMGPLELAD IGLDV
Sbjct: 181 CSQDYAGFVVNRILMPMINEAFHTLYTGVATKEDIDSGMKHGTNHPMGPLELADLIGLDV 240

Query: 241 CLSIMKVLHSGLGDNKYAPCPLLVQYVDAGRLGRKRGIGVYDYRKLPQSVKSK 280
           CLS+MKVLH GLGD+KYAPCPLLVQYVDAGRLGRKRG+GVYDYR+  Q +  +
Sbjct: 241 CLSVMKVLHEGLGDSKYAPCPLLVQYVDAGRLGRKRGVGVYDYREATQKLSPR 293

[Close]
BLAST of Lsi05G001390 vs. TAIR 10
Match: AT3G06860.1 (multifunctional protein 2 )

HSP 1 Score: 109.8 bits (273), Expect = 3.7e-24
Identity = 83/286 (29.02%), Postives = 137/286 (47.90%), Query Frame = 0 

Query: 3   EIKAIGVVGGGQMGSGIAQLAATHGHEVWLIDSDPAALAKASDSISSSIQRLVSKNLLSR 62
           +IK + ++GGG MGSGIA       + V L + +   L      + +++Q  V K  +S+
Sbjct: 311 KIKKVAIIGGGLMGSGIATALILSNYPVILKEVNEKFLEAGIGRVKANLQSRVRKGSMSQ 370

Query: 63  EVGVIALQRLKFSTDLKELHSADVIIEAIVESEDVKRKLFLELDKIAKRSSILASNTSSI 122
           E     +  LK S D +     D++IEA++E+  +K+++F +L+K   +  ILASNTS+I
Sbjct: 371 EKFEKTMSLLKGSLDYESFRDVDMVIEAVIENISLKQQIFADLEKYCPQHCILASNTSTI 430

Query: 123 PITRLASATTRPDQLV--------------EIVRGADTSNKTFDAIKILAERLGKTVICS 182
            + ++   T   D++V              EIVR   TS +    +  + +++ KT +  
Sbjct: 431 DLNKIGERTKSQDRIVGAHFFSPAHIMPLLEIVRTNHTSAQVIVDLLDVGKKIKKTPVVV 490

Query: 183 QDYSGFIVNRILMPMINEAFFALYTGVATKEDIDTGM-KLGTNHPMGPLELADFIGLDVC 242
            + +GF VNR+  P    A F +  G A    ID  + K G   PMGP  L D +G  V 
Sbjct: 491 GNCTGFAVNRMFFPYTQAAMFLVECG-ADPYLIDRAISKFGM--PMGPFRLCDLVGFGVA 550

Query: 243 LSIMKVLHSGLGDNKYAP--CPLLVQYVDAGRLGRKRGIGVYDYRK 272
           ++          +  Y     PL+ +   AG   RK G  +YD ++
Sbjct: 551 IATATQFIENFSERTYKSMIIPLMQEDKRAGEATRK-GFYLYDDKR 592

[Close]
BLAST of Lsi05G001390 vs. TAIR 10
Match: AT4G29010.1 (Enoyl-CoA hydratase/isomerase family )

HSP 1 Score: 109.4 bits (272), Expect = 4.8e-24
Identity = 81/282 (28.72%), Postives = 130/282 (46.10%), Query Frame = 0 

Query: 4   IKAIGVVGGGQMGSGIAQLAATHGHEVWLIDSDPAALAKASDSISSSIQRLVSKNLLSRE 63
           IK + V+GGG MGSGIA         V L + +   L K   S+ ++++ LVS+  L+++
Sbjct: 309 IKKVAVIGGGLMGSGIATALLLSNIRVVLKEINSEFLMKGIKSVEANMKSLVSRGKLTQD 368

Query: 64  VGVIALQRLKFSTDLKELHSADVIIEAIVESEDVKRKLFLELDKIAKRSSILASNTSSIP 123
               AL   K   D  E +  D++IEA++E+  +K+ +F E++K+     ILASNTS+I 
Sbjct: 369 KAGKALSLFKGVLDYTEFNDVDMVIEAVIENIQLKQNIFKEIEKVCSPHCILASNTSTID 428

Query: 124 ITRLASATTRPDQLV--------------EIVRGADTSNKTFDAIKILAERLGKTVICSQ 183
           +  +   T   D++V              EIVR  +TS +    +  + + + K  +   
Sbjct: 429 LDVIGEKTNSKDRIVGAHFFSPAHLMPLLEIVRSKNTSAQVILDLMAVGKAIKKVPVVVG 488

Query: 184 DYSGFIVNRILMPMINEAFFALYTGVATKEDIDTGMKLGTNHPMGPLELADFIGLDVCLS 243
           +  GF VNR   P    A      GV            G   P+GP +L D  G  + L+
Sbjct: 489 NCIGFAVNRTFFPYSQAAHMLANLGVDLFRIDSVITSFGL--PLGPFQLGDLAGHGIGLA 548

Query: 244 IMKVLHSGLGDNKYAPCPLLVQYVDAGRLGRKRGIGVYDYRK 272
           +  +     GD  +   P+    + +GR G+  G G Y Y K
Sbjct: 549 VGPIYAKVYGDRMFR-SPMTELLLKSGRNGKINGRGYYIYEK 587

The following BLAST results are available for this feature:
BLAST of Lsi05G001390 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q452232.3e-7152.313-hydroxybutyryl-CoA dehydrogenase OS=Bradyrhizobium diazoefficiens (strain JCM ... [more]
P458566.5e-6649.12Probable 3-hydroxybutyryl-CoA dehydrogenase OS=Bacillus subtilis (strain 168) OX... [more]
P520417.9e-6448.943-hydroxybutyryl-CoA dehydrogenase OS=Clostridium acetobutylicum (strain ATCC 82... [more]
Q9RVG11.4e-6350.00Probable 3-hydroxybutyryl-CoA dehydrogenase OS=Deinococcus radiodurans (strain A... [more]
P778514.1e-6046.263-hydroxybutyryl-CoA dehydrogenase OS=Thermoanaerobacterium thermosaccharolyticu... [more]
BLAST of Lsi05G001390 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A5D3CH808.3e-14190.883-hydroxybutyryl-CoA dehydrogenase OS=Cucumis melo var. makuwa OX=1194695 GN=E56... [more]
A0A5A7USC99.2e-14090.203-hydroxybutyryl-CoA dehydrogenase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C... [more]
A0A1S3C2Y79.2e-14090.203-hydroxybutyryl-CoA dehydrogenase OS=Cucumis melo OX=3656 GN=LOC103496074 PE=3 ... [more]
A0A0A0L6N57.8e-13989.86Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G234000 PE=3 SV=1[more]
A0A6J1CM786.4e-13387.07uncharacterized protein LOC111012899 OS=Momordica charantia OX=3673 GN=LOC111012... [more]
BLAST of Lsi05G001390 vs. NCBI nr
Match NameE-valueIdentityDescription
TYK11243.11.7e-14090.883-hydroxybutyryl-CoA dehydrogenase [Cucumis melo var. makuwa][more]
XP_038878166.11.7e-14091.223-hydroxybutyryl-CoA dehydrogenase [Benincasa hispida][more]
XP_008456022.11.9e-13990.20PREDICTED: 3-hydroxybutyryl-CoA dehydrogenase [Cucumis melo] >KAA0058823.1 3-hyd... [more]
XP_004146246.11.6e-13889.86uncharacterized protein LOC101221475 isoform X1 [Cucumis sativus][more]
XP_023553026.15.9e-13386.15uncharacterized protein LOC111810545 [Cucurbita pepo subsp. pepo][more]
BLAST of Lsi05G001390 vs. TAIR 10
Match NameE-valueIdentityDescription
AT3G15290.13.1e-11169.623-hydroxyacyl-CoA dehydrogenase family protein [more]
AT3G06860.13.7e-2429.02multifunctional protein 2 [more]
AT4G29010.14.8e-2428.72Enoyl-CoA hydratase/isomerase family [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR0226943-hydroxyacyl-CoA dehydrogenasePIRSFPIRSF000105HCDHcoord: 1..281
e-value: 4.3E-73
score: 244.2
IPR0061763-hydroxyacyl-CoA dehydrogenase, NAD bindingPFAMPF027373HCDH_Ncoord: 7..170
e-value: 8.2E-45
score: 152.9
IPR0061083-hydroxyacyl-CoA dehydrogenase, C-terminalPFAMPF007253HCDHcoord: 173..269
e-value: 3.1E-35
score: 120.6
NoneNo IPR availableGENE3D3.40.50.720coord: 1..175
e-value: 1.8E-49
score: 170.1
NoneNo IPR availablePIRSRPIRSR500134-3PIRSR500134-3coord: 7..92
e-value: 0.0017
score: 15.1
NoneNo IPR availablePANTHERPTHR480753-HYDROXYACYL-COA DEHYDROGENASE FAMILY PROTEINcoord: 3..274
NoneNo IPR availablePANTHERPTHR48075:SF53-HYDROXYACYL-COA DEHYDROGENASE FAMILY PROTEINcoord: 3..274
IPR0133286-phosphogluconate dehydrogenase, domain 2GENE3D1.10.1040.10coord: 176..281
e-value: 4.4E-33
score: 116.1
IPR0061803-hydroxyacyl-CoA dehydrogenase, conserved sitePROSITEPS000673HCDHcoord: 170..195
IPR036291NAD(P)-binding domain superfamilySUPERFAMILY51735NAD(P)-binding Rossmann-fold domainscoord: 1..171
IPR0089276-phosphogluconate dehydrogenase-like, C-terminal domain superfamilySUPERFAMILY481796-phosphogluconate dehydrogenase C-terminal domain-likecoord: 173..271

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi05G001390.1Lsi05G001390.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006631 fatty acid metabolic process
molecular_function GO:0004300 enoyl-CoA hydratase activity
molecular_function GO:0070403 NAD+ binding
molecular_function GO:0016616 oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
molecular_function GO:0016491 oxidoreductase activity