Lsi05G000450 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi05G000450
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	Plant invertase/pectin methylesterase inhibitor superfamily protein, putative
Location	chr05: 965787 .. 966338 (+)
RNA-Seq Expression	Lsi05G000450
Synteny	Lsi05G000450

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAAAATCCCATTTCAAAATCAATTTCATTACTTCTTTTCATTCCAACATTTCTTCAATTAGCATATTGTTTAGACATTGTTCAACAGTCTTGCAAATTGGCTGCACAAACAGACCCATATGTTAACTACAAACTATGTGTTCAAAGCCTTAAAGCCAATCCCAATTATTCCAAAGCTGCAAATTTCAAAGACTTGCTTATGATTTCAATCAACCACTCAAAGGCCAATGCCACAGAAATTGGGTCACAGATTTCAGAGCTTTTGAAAGGAAGTAATGAGAAATTGGGGAAATATTCATTGAATTGTTTGAAAAGCTGTTTAGAACTTTATTCTGAGGCTATTTCTGATTTGGAAAAGGCTTTAGGAGCTTTGAAAATGGAGGATTATGAGACAGCAAATACAGGGGTTAGTGCAGCCATGGATGCACCAATAAGTTGTGAAGATGGGTATAGGGAAAAAGATGGAGAAGTTTCTCCTTTCACAGAGATCAATGATGGGTTCTTTCAATTGATTGCAATTTCTCTTGCTTTTATTAATATGTCTAAGTAA

mRNA sequence

ATGGAAAATCCCATTTCAAAATCAATTTCATTACTTCTTTTCATTCCAACATTTCTTCAATTAGCATATTGTTTAGACATTGTTCAACAGTCTTGCAAATTGGCTGCACAAACAGACCCATATGTTAACTACAAACTATGTGTTCAAAGCCTTAAAGCCAATCCCAATTATTCCAAAGCTGCAAATTTCAAAGACTTGCTTATGATTTCAATCAACCACTCAAAGGCCAATGCCACAGAAATTGGGTCACAGATTTCAGAGCTTTTGAAAGGAAGTAATGAGAAATTGGGGAAATATTCATTGAATTGTTTGAAAAGCTGTTTAGAACTTTATTCTGAGGCTATTTCTGATTTGGAAAAGGCTTTAGGAGCTTTGAAAATGGAGGATTATGAGACAGCAAATACAGGGGTTAGTGCAGCCATGGATGCACCAATAAGTTGTGAAGATGGGTATAGGGAAAAAGATGGAGAAGTTTCTCCTTTCACAGAGATCAATGATGGGTTCTTTCAATTGATTGCAATTTCTCTTGCTTTTATTAATATGTCTAAGTAA

Coding sequence (CDS)

ATGGAAAATCCCATTTCAAAATCAATTTCATTACTTCTTTTCATTCCAACATTTCTTCAATTAGCATATTGTTTAGACATTGTTCAACAGTCTTGCAAATTGGCTGCACAAACAGACCCATATGTTAACTACAAACTATGTGTTCAAAGCCTTAAAGCCAATCCCAATTATTCCAAAGCTGCAAATTTCAAAGACTTGCTTATGATTTCAATCAACCACTCAAAGGCCAATGCCACAGAAATTGGGTCACAGATTTCAGAGCTTTTGAAAGGAAGTAATGAGAAATTGGGGAAATATTCATTGAATTGTTTGAAAAGCTGTTTAGAACTTTATTCTGAGGCTATTTCTGATTTGGAAAAGGCTTTAGGAGCTTTGAAAATGGAGGATTATGAGACAGCAAATACAGGGGTTAGTGCAGCCATGGATGCACCAATAAGTTGTGAAGATGGGTATAGGGAAAAAGATGGAGAAGTTTCTCCTTTCACAGAGATCAATGATGGGTTCTTTCAATTGATTGCAATTTCTCTTGCTTTTATTAATATGTCTAAGTAA

Protein sequence

MENPISKSISLLLFIPTFLQLAYCLDIVQQSCKLAAQTDPYVNYKLCVQSLKANPNYSKAANFKDLLMISINHSKANATEIGSQISELLKGSNEKLGKYSLNCLKSCLELYSEAISDLEKALGALKMEDYETANTGVSAAMDAPISCEDGYREKDGEVSPFTEINDGFFQLIAISLAFINMSK

Homology

[Close]

BLAST of Lsi05G000450 vs. ExPASy Swiss-Prot
Match: Q8GT41 (Putative invertase inhibitor OS=Platanus acerifolia OX=140101 PE=1 SV=1)

HSP 1 Score: 111.3 bits (277), Expect = 1.2e-23
Identity = 72/178 (40.45%), Postives = 105/178 (58.99%), Query Frame = 0


Query: 8   SISLLLF---IPTFLQLAYCLDIVQQSCKLAAQTDPYVNYKLCVQSLKANPNYSKAANFK 67
           S SL +F   +   LQ     DIVQ +CK  AQ  P VNY  CV+SL A+P  S  A+ +
Sbjct: 4   SFSLCIFFFNLLLLLQAVISADIVQGTCKKVAQRSPNVNYDFCVKSLGADPK-SHTADLQ 63

Query: 68  DLLMISINHSKANATEIGSQISELLKGS-NEKLGKYSLNCLKSCLELYSEAISDLEKALG 127
            L +IS N +  + ++I + I  +LK   +  L KY    L  C+ LY++A S +++A+ 
Sbjct: 64  GLGVISANLAIQHGSKIQTFIGRILKSKVDPALKKY----LNDCVGLYADAKSSVQEAIA 123

Query: 128 ALKMEDYETANTGVSAAMDAPISCEDGYREKDGEVSPFTEINDGFFQLIAISLAFINM 182
             K +DY +AN  +SAA+D  ++CEDG++EK G VSP T+ N  + QL AISLA   +
Sbjct: 124 DFKSKDYASANVKMSAALDDSVTCEDGFKEKKGIVSPVTKENKDYVQLTAISLAITKL 176

[Close]

BLAST of Lsi05G000450 vs. ExPASy Swiss-Prot
Match: A9YUH4 (Putative invertase inhibitor OS=Platanus orientalis OX=122832 PE=1 SV=1)

HSP 1 Score: 108.6 bits (270), Expect = 7.5e-23
Identity = 65/157 (41.40%), Postives = 96/157 (61.15%), Query Frame = 0


Query: 26  DIVQQSCKLAAQTDPYVNYKLCVQSLKANPNYSKAANFKDLLMISINHSKANATEIGSQI 85
           DIVQ +CK  AQ  P VNY  CV+SL A+P  S +A+ + L +IS N +    ++I + I
Sbjct: 16  DIVQGTCKKVAQRSPNVNYDFCVKSLGADPK-SHSADLQGLGVISANLAIQQGSKIQTFI 75

Query: 86  SELLKGS-NEKLGKYSLNCLKSCLELYSEAISDLEKALGALKMEDYETANTGVSAAMDAP 145
             +LK   +  L KY    L  C+ LY++A S +++A+   K +DY +AN  +SAA+D  
Sbjct: 76  GRILKSKVDPALKKY----LNDCVGLYADAKSSVQEAIADFKSKDYASANVKMSAALDDS 135

Query: 146 ISCEDGYREKDGEVSPFTEINDGFFQLIAISLAFINM 182
           ++CEDG++EK G  SP T+ N  + QL AISLA   +
Sbjct: 136 VTCEDGFKEKKGIASPVTKENKDYVQLTAISLAITKL 167

[Close]

BLAST of Lsi05G000450 vs. ExPASy Swiss-Prot
Match: Q9FJR5 (Pectinesterase inhibitor 12 OS=Arabidopsis thaliana OX=3702 GN=PMEI12 PE=2 SV=1)

HSP 1 Score: 94.4 bits (233), Expect = 1.5e-18
Identity = 60/173 (34.68%), Postives = 91/173 (52.60%), Query Frame = 0


Query: 9   ISLLLFIPTFLQLAYCLDIVQQSCKLAAQTDPYVNYKLCVQSLKANPNYSKAANFKDLLM 68
           +SL++F       A    ++Q SCK A   DP ++Y  CV SL  +P    A   + L++
Sbjct: 5   VSLVIFSLFLNGFATAQTLIQDSCKKAFAKDPQLSYDFCVNSLTQDPQSKAATTLESLVL 64

Query: 69  ISINHSKANATEIGSQISELLKGSNEKLGKYSLNCLKSCLELYSEAISDLEKALGALKME 128
            S   + A  T +   +++ LK   ++  +  +  LK CL  Y++A  DL  AL  +K  
Sbjct: 65  ASTKTAAAKITNLKGIVAQDLK---DQRYQDIVEDLKLCLGFYNDANDDLTTALANIKSR 124

Query: 129 DYETANTGVSAAMDAPISCEDGYREKDGEVSPFTEINDGFFQLIAISLAFINM 182
           DY+ AN  +SAA+D P +CED ++E   + SP T  N   F+ I I LAF NM
Sbjct: 125 DYQGANINLSAALDVPGNCEDDFKEAK-KTSPITNENSILFKTILIPLAFTNM 173

[Close]

BLAST of Lsi05G000450 vs. ExPASy Swiss-Prot
Match: Q9LUV1 (Pectinesterase inhibitor 2 OS=Arabidopsis thaliana OX=3702 GN=PMEI2 PE=1 SV=1)

HSP 1 Score: 53.5 bits (127), Expect = 2.9e-06
Identity = 45/140 (32.14%), Postives = 65/140 (46.43%), Query Frame = 0


Query: 43  NYKLCVQSLKANPNYSKAANFKDLLMISINHSKANATEIGSQISELLKGSNEKLGKYSLN 102
           N   C   +K+NP  S  A+ + L  I+   ++ +A+E   +I  L+K +     K    
Sbjct: 39  NQSFCTSYMKSNPKTS-GADLQTLANITFGSAQTSASEGFRKIQSLVKTATNPTMK---K 98

Query: 103 CLKSCLELYSEAISDLEKALGALKMEDYETANTGVSAAMDAPISCEDGYREKDGEVSPFT 162
              SC++ Y  AIS L  A  +L   D +  N  VSAAM+ P +CE      D +V P  
Sbjct: 99  AYTSCVQHYKSAISSLNDAKQSLASGDGKGLNIKVSAAMEGPSTCEQDM--ADFKVDPSA 158

Query: 163 EINDGFFQ-LIAISLAFINM 182
             N G FQ +  I L   NM
Sbjct: 159 VKNSGDFQNICGIVLVISNM 172

[Close]

BLAST of Lsi05G000450 vs. ExPASy TrEMBL
Match: A0A0A0LHE9 (PMEI domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G910710 PE=4 SV=1)

HSP 1 Score: 283.9 bits (725), Expect = 4.8e-73
Identity = 145/181 (80.11%), Postives = 163/181 (90.06%), Query Frame = 0


Query: 1   MENPISKSISLLLFIPTFLQLAYCLDIVQQSCKLAAQTDPYVNYKLCVQSLKANPNYSKA 60
           MENP S+SI  LL +PT +QLAY LDIVQ SCKLAA+TDPYV+YKLCVQ+LKA+PN SK 
Sbjct: 1   MENPTSRSILFLLCVPTLIQLAYGLDIVQHSCKLAAKTDPYVDYKLCVQTLKASPN-SKD 60

Query: 61  ANFKDLLMISINHSKANATEIGSQISELLKGSNEKLGKYSLNCLKSCLELYSEAISDLEK 120
           A FKDL++ISIN SKANATEIGS+ISEL+K  +EK G+YSLNCLKSCLELYSEA+SDLEK
Sbjct: 61  AEFKDLVVISINQSKANATEIGSEISELMKRRSEKWGQYSLNCLKSCLELYSEAVSDLEK 120

Query: 121 ALGALKMEDYETANTGVSAAMDAPISCEDGYREKDGEVSPFTEINDGFFQLIAISLAFIN 180
           AL  LKMEDYETA TGVSAAMDAP+SCEDGY+EKDGEVSP +EINDGFFQL+AISLAFIN
Sbjct: 121 ALKGLKMEDYETAKTGVSAAMDAPVSCEDGYKEKDGEVSPLSEINDGFFQLVAISLAFIN 180

Query: 181 M 182
           M
Sbjct: 181 M 180

[Close]

BLAST of Lsi05G000450 vs. ExPASy TrEMBL
Match: A0A5A7TAE5 (Putative invertase inhibitor OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold455G004030 PE=4 SV=1)

HSP 1 Score: 274.6 bits (701), Expect = 2.9e-70
Identity = 141/181 (77.90%), Postives = 161/181 (88.95%), Query Frame = 0


Query: 1   MENPISKSISLLLFIPTFLQLAYCLDIVQQSCKLAAQTDPYVNYKLCVQSLKANPNYSKA 60
           MENPI+KSI  LL +PT +QLA  LDIVQ  CKLAA+TDPYV+YKLCVQ+LKANPN S+ 
Sbjct: 1   MENPIAKSILFLLCVPTLIQLANGLDIVQHYCKLAAKTDPYVDYKLCVQTLKANPN-SED 60

Query: 61  ANFKDLLMISINHSKANATEIGSQISELLKGSNEKLGKYSLNCLKSCLELYSEAISDLEK 120
           A+FKDL+MISIN +KANATEI S+ISEL+K ++EK GKYSLNCLKSCLELYSEA+SDL+K
Sbjct: 61  ADFKDLVMISINQAKANATEIRSEISELMKRTSEKWGKYSLNCLKSCLELYSEAVSDLKK 120

Query: 121 ALGALKMEDYETANTGVSAAMDAPISCEDGYREKDGEVSPFTEINDGFFQLIAISLAFIN 180
           AL  LKMEDYETA T VSAAMDAP+SCEDGY+EKDGEVSP +E NDGFFQL+AISLAFIN
Sbjct: 121 ALRGLKMEDYETAKTAVSAAMDAPVSCEDGYKEKDGEVSPLSETNDGFFQLVAISLAFIN 180

Query: 181 M 182
           M
Sbjct: 181 M 180

[Close]

BLAST of Lsi05G000450 vs. ExPASy TrEMBL
Match: A0A1S3CQ13 (putative invertase inhibitor OS=Cucumis melo OX=3656 GN=LOC103503498 PE=4 SV=1)

HSP 1 Score: 274.6 bits (701), Expect = 2.9e-70
Identity = 141/181 (77.90%), Postives = 161/181 (88.95%), Query Frame = 0


Query: 1   MENPISKSISLLLFIPTFLQLAYCLDIVQQSCKLAAQTDPYVNYKLCVQSLKANPNYSKA 60
           MENPI+KSI  LL +PT +QLA  LDIVQ  CKLAA+TDPYV+YKLCVQ+LKANPN S+ 
Sbjct: 1   MENPIAKSILFLLCVPTLIQLANGLDIVQHYCKLAAKTDPYVDYKLCVQTLKANPN-SED 60

Query: 61  ANFKDLLMISINHSKANATEIGSQISELLKGSNEKLGKYSLNCLKSCLELYSEAISDLEK 120
           A+FKDL+MISIN +KANATEI S+ISEL+K ++EK GKYSLNCLKSCLELYSEA+SDL+K
Sbjct: 61  ADFKDLVMISINQAKANATEIRSEISELMKRTSEKWGKYSLNCLKSCLELYSEAVSDLKK 120

Query: 121 ALGALKMEDYETANTGVSAAMDAPISCEDGYREKDGEVSPFTEINDGFFQLIAISLAFIN 180
           AL  LKMEDYETA T VSAAMDAP+SCEDGY+EKDGEVSP +E NDGFFQL+AISLAFIN
Sbjct: 121 ALRGLKMEDYETAKTAVSAAMDAPVSCEDGYKEKDGEVSPLSETNDGFFQLVAISLAFIN 180

Query: 181 M 182
           M
Sbjct: 181 M 180

[Close]

BLAST of Lsi05G000450 vs. ExPASy TrEMBL
Match: A0A6J1ICI2 (putative invertase inhibitor OS=Cucurbita maxima OX=3661 GN=LOC111471816 PE=4 SV=1)

HSP 1 Score: 247.3 bits (630), Expect = 5.0e-62
Identity = 134/184 (72.83%), Postives = 154/184 (83.70%), Query Frame = 0


Query: 1   MENPISKSISLLLF-IPTFLQLAYCLDIVQQSCKLAAQTDPYVNYKLCVQSLKANPNYSK 60
           M++PIS  I LLL  +PT   LA+ LDI+Q+SCK AA+TDPYV+YKLCVQ+L+ANPN SK
Sbjct: 1   MKSPISNPILLLLLSLPTPFHLAFSLDIIQRSCKSAAKTDPYVDYKLCVQTLRANPN-SK 60

Query: 61  AANFKDLLMISINHSKANATEIGSQISELLKGSNE-KLGKYSLNCLKSCLELYSEAISDL 120
           AA FK+L++ISI  SKANATEIGS+ISELLKGS     GKYSL CLK CLE+YSEA+S L
Sbjct: 61  AATFKELVLISITQSKANATEIGSEISELLKGSERWGKGKYSLGCLKGCLEIYSEAVSGL 120

Query: 121 EKALGALKMEDYETANTGVSAAMDAPISCEDGYREKDGEVSPFTEINDGFFQLIAISLAF 180
           E ALGA+KM DYETAN  VSAAMDA +SCEDGY+EKDGEVSP TEIN GFFQL AISLAF
Sbjct: 121 ELALGAVKMGDYETANIKVSAAMDAALSCEDGYKEKDGEVSPLTEINCGFFQLNAISLAF 180

Query: 181 INMS 183
           INM+
Sbjct: 181 INMA 183

[Close]

BLAST of Lsi05G000450 vs. ExPASy TrEMBL
Match: A0A6J1H1W1 (putative invertase inhibitor OS=Cucurbita moschata OX=3662 GN=LOC111459677 PE=4 SV=1)

HSP 1 Score: 230.7 bits (587), Expect = 4.8e-57
Identity = 122/181 (67.40%), Postives = 146/181 (80.66%), Query Frame = 0


Query: 1   MENPISKSISLLLFIPTFLQLAYCLDIVQQSCKLAAQTDPYVNYKLCVQSLKANPNYSKA 60
           M+   S SI +L  + T  Q  +CLDIVQ+SCKLAA+ DPYV+YKLCV+SL+ANP+ SK 
Sbjct: 1   MKTHFSTSILMLFSLLTLFQFGFCLDIVQRSCKLAAKRDPYVDYKLCVRSLRANPS-SKD 60

Query: 61  ANFKDLLMISINHSKANATEIGSQISELLKGSNEKLGKYSLNCLKSCLELYSEAISDLEK 120
           A FKDL++ISI  SKANAT IGS+I+ LLKG   K GKYS +CL+SCLE+YSEA+SDL+ 
Sbjct: 61  ATFKDLVLISIEQSKANATAIGSEITGLLKGGG-KWGKYSTSCLESCLEVYSEAVSDLKL 120

Query: 121 ALGALKMEDYETANTGVSAAMDAPISCEDGYREKDGEVSPFTEINDGFFQLIAISLAFIN 180
            L A+KM DYETANT VSAAMDAP++CEDGY EKDGEVSP TE N GFFQL AISLAFI+
Sbjct: 121 GLVAVKMGDYETANTEVSAAMDAPLTCEDGYNEKDGEVSPLTEKNGGFFQLTAISLAFIS 179

Query: 181 M 182
           +
Sbjct: 181 L 179

[Close]

BLAST of Lsi05G000450 vs. NCBI nr
Match: XP_038888305.1 (putative invertase inhibitor [Benincasa hispida])

HSP 1 Score: 287.7 bits (735), Expect = 6.9e-74
Identity = 147/183 (80.33%), Postives = 164/183 (89.62%), Query Frame = 0


Query: 1   MENPISKSISLLLFIPTFLQLAYCLDIVQQSCKLAAQTDPYVNYKLCVQSLKANPNYSKA 60
           M++  +KSI  LL +PT  QLAYCLDIVQ SCKLAA+TDPYV+YKLCVQ LKANPN SK 
Sbjct: 1   MKDLTTKSILFLLCVPTLFQLAYCLDIVQHSCKLAAKTDPYVDYKLCVQILKANPN-SKD 60

Query: 61  ANFKDLLMISINHSKANATEIGSQISELLKGSNEKLGKYSLNCLKSCLELYSEAISDLEK 120
           ANFKDL+MISIN SKANATEI S+IS+L+KG+NEK GKYSLNCLKSCLELYSEA+SDL+K
Sbjct: 61  ANFKDLVMISINQSKANATEIRSEISQLIKGTNEKWGKYSLNCLKSCLELYSEAVSDLKK 120

Query: 121 ALGALKMEDYETANTGVSAAMDAPISCEDGYREKDGEVSPFTEINDGFFQLIAISLAFIN 180
           AL AL+MEDYET NT VSAAMDAP+SCEDGY+EKDGEVSP TEINDGFFQLIAISLAF+N
Sbjct: 121 ALRALEMEDYETTNTEVSAAMDAPVSCEDGYKEKDGEVSPLTEINDGFFQLIAISLAFVN 180

Query: 181 MSK 184
           M K
Sbjct: 181 MCK 182

[Close]

BLAST of Lsi05G000450 vs. NCBI nr
Match: XP_004148520.1 (putative invertase inhibitor [Cucumis sativus])

HSP 1 Score: 283.9 bits (725), Expect = 1.0e-72
Identity = 145/181 (80.11%), Postives = 163/181 (90.06%), Query Frame = 0


Query: 1   MENPISKSISLLLFIPTFLQLAYCLDIVQQSCKLAAQTDPYVNYKLCVQSLKANPNYSKA 60
           MENP S+SI  LL +PT +QLAY LDIVQ SCKLAA+TDPYV+YKLCVQ+LKA+PN SK 
Sbjct: 1   MENPTSRSILFLLCVPTLIQLAYGLDIVQHSCKLAAKTDPYVDYKLCVQTLKASPN-SKD 60

Query: 61  ANFKDLLMISINHSKANATEIGSQISELLKGSNEKLGKYSLNCLKSCLELYSEAISDLEK 120
           A FKDL++ISIN SKANATEIGS+ISEL+K  +EK G+YSLNCLKSCLELYSEA+SDLEK
Sbjct: 61  AEFKDLVVISINQSKANATEIGSEISELMKRRSEKWGQYSLNCLKSCLELYSEAVSDLEK 120

Query: 121 ALGALKMEDYETANTGVSAAMDAPISCEDGYREKDGEVSPFTEINDGFFQLIAISLAFIN 180
           AL  LKMEDYETA TGVSAAMDAP+SCEDGY+EKDGEVSP +EINDGFFQL+AISLAFIN
Sbjct: 121 ALKGLKMEDYETAKTGVSAAMDAPVSCEDGYKEKDGEVSPLSEINDGFFQLVAISLAFIN 180

Query: 181 M 182
           M
Sbjct: 181 M 180

[Close]

BLAST of Lsi05G000450 vs. NCBI nr
Match: XP_008465919.1 (PREDICTED: putative invertase inhibitor [Cucumis melo] >KAA0038527.1 putative invertase inhibitor [Cucumis melo var. makuwa] >TYK31124.1 putative invertase inhibitor [Cucumis melo var. makuwa])

HSP 1 Score: 274.6 bits (701), Expect = 6.0e-70
Identity = 141/181 (77.90%), Postives = 161/181 (88.95%), Query Frame = 0


Query: 1   MENPISKSISLLLFIPTFLQLAYCLDIVQQSCKLAAQTDPYVNYKLCVQSLKANPNYSKA 60
           MENPI+KSI  LL +PT +QLA  LDIVQ  CKLAA+TDPYV+YKLCVQ+LKANPN S+ 
Sbjct: 1   MENPIAKSILFLLCVPTLIQLANGLDIVQHYCKLAAKTDPYVDYKLCVQTLKANPN-SED 60

Query: 61  ANFKDLLMISINHSKANATEIGSQISELLKGSNEKLGKYSLNCLKSCLELYSEAISDLEK 120
           A+FKDL+MISIN +KANATEI S+ISEL+K ++EK GKYSLNCLKSCLELYSEA+SDL+K
Sbjct: 61  ADFKDLVMISINQAKANATEIRSEISELMKRTSEKWGKYSLNCLKSCLELYSEAVSDLKK 120

Query: 121 ALGALKMEDYETANTGVSAAMDAPISCEDGYREKDGEVSPFTEINDGFFQLIAISLAFIN 180
           AL  LKMEDYETA T VSAAMDAP+SCEDGY+EKDGEVSP +E NDGFFQL+AISLAFIN
Sbjct: 121 ALRGLKMEDYETAKTAVSAAMDAPVSCEDGYKEKDGEVSPLSETNDGFFQLVAISLAFIN 180

Query: 181 M 182
           M
Sbjct: 181 M 180

[Close]

BLAST of Lsi05G000450 vs. NCBI nr
Match: KAE8651458.1 (hypothetical protein Csa_001325 [Cucumis sativus])

HSP 1 Score: 263.5 bits (672), Expect = 1.4e-66
Identity = 134/169 (79.29%), Postives = 151/169 (89.35%), Query Frame = 0


Query: 1   MENPISKSISLLLFIPTFLQLAYCLDIVQQSCKLAAQTDPYVNYKLCVQSLKANPNYSKA 60
           MENP S+SI  LL +PT +QLAY LDIVQ SCKLAA+TDPYV+YKLCVQ+LKA+PN SK 
Sbjct: 1   MENPTSRSILFLLCVPTLIQLAYGLDIVQHSCKLAAKTDPYVDYKLCVQTLKASPN-SKD 60

Query: 61  ANFKDLLMISINHSKANATEIGSQISELLKGSNEKLGKYSLNCLKSCLELYSEAISDLEK 120
           A FKDL++ISIN SKANATEIGS+ISEL+K  +EK G+YSLNCLKSCLELYSEA+SDLEK
Sbjct: 61  AEFKDLVVISINQSKANATEIGSEISELMKRRSEKWGQYSLNCLKSCLELYSEAVSDLEK 120

Query: 121 ALGALKMEDYETANTGVSAAMDAPISCEDGYREKDGEVSPFTEINDGFF 170
           AL  LKMEDYETA TGVSAAMDAP+SCEDGY+EKDGEVSP +EINDGFF
Sbjct: 121 ALKGLKMEDYETAKTGVSAAMDAPVSCEDGYKEKDGEVSPLSEINDGFF 168

[Close]

BLAST of Lsi05G000450 vs. NCBI nr
Match: XP_022973258.1 (putative invertase inhibitor [Cucurbita maxima])

HSP 1 Score: 247.3 bits (630), Expect = 1.0e-61
Identity = 134/184 (72.83%), Postives = 154/184 (83.70%), Query Frame = 0


Query: 1   MENPISKSISLLLF-IPTFLQLAYCLDIVQQSCKLAAQTDPYVNYKLCVQSLKANPNYSK 60
           M++PIS  I LLL  +PT   LA+ LDI+Q+SCK AA+TDPYV+YKLCVQ+L+ANPN SK
Sbjct: 1   MKSPISNPILLLLLSLPTPFHLAFSLDIIQRSCKSAAKTDPYVDYKLCVQTLRANPN-SK 60

Query: 61  AANFKDLLMISINHSKANATEIGSQISELLKGSNE-KLGKYSLNCLKSCLELYSEAISDL 120
           AA FK+L++ISI  SKANATEIGS+ISELLKGS     GKYSL CLK CLE+YSEA+S L
Sbjct: 61  AATFKELVLISITQSKANATEIGSEISELLKGSERWGKGKYSLGCLKGCLEIYSEAVSGL 120

Query: 121 EKALGALKMEDYETANTGVSAAMDAPISCEDGYREKDGEVSPFTEINDGFFQLIAISLAF 180
           E ALGA+KM DYETAN  VSAAMDA +SCEDGY+EKDGEVSP TEIN GFFQL AISLAF
Sbjct: 121 ELALGAVKMGDYETANIKVSAAMDAALSCEDGYKEKDGEVSPLTEINCGFFQLNAISLAF 180

Query: 181 INMS 183
           INM+
Sbjct: 181 INMA 183

[Close]

BLAST of Lsi05G000450 vs. TAIR 10
Match: AT5G46940.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 118.6 bits (296), Expect = 5.2e-27
Identity = 68/179 (37.99%), Postives = 103/179 (57.54%), Query Frame = 0


Query: 9   ISLLLFIPTFLQLAYCL----DIVQQSCKLAAQTDPYVNYKLCVQSLKANPNYSKAANFK 68
           +  LL++ TF  L+  L     +++ SCK A  T P   Y LCV SL+ NP    A +  
Sbjct: 1   MKFLLYLVTFFVLSNGLANGQTLIRNSCKKATATSPKFKYNLCVTSLETNPQAKTAKDLA 60

Query: 69  DLLMISINHSKANATEIGSQISELLKGSNEKLGKYSLNCLKSCLELYSEAISDLEKALGA 128
            L+M S  ++   AT +   + +++KG  +K+ K +   L+ CL+LY++AI  L +AL  
Sbjct: 61  GLVMASTKNAVTKATTLKGTVDKIIKG--KKVNKMTAMPLRDCLQLYTDAIGSLNEALAG 120

Query: 129 LKMEDYETANTGVSAAMDAPISCEDGYREKDGEVSPFTEINDGFFQLIAISLAFINMSK 184
           +K  +Y T  T +SAAMD P +CE G++E+    SP T+ ND  +Q+I I LAF NM K
Sbjct: 121 VKSRNYPTVKTVLSAAMDTPSTCETGFKERKAP-SPVTKENDNLYQMILIPLAFTNMLK 176

[Close]

BLAST of Lsi05G000450 vs. TAIR 10
Match: AT5G46930.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 104.8 bits (260), Expect = 7.7e-23
Identity = 61/175 (34.86%), Postives = 98/175 (56.00%), Query Frame = 0


Query: 8   SISLLLFIPTFLQLAYCLDIVQQSCKLAAQTDPYVNYKLCVQSLKANPNYSKAANFKDLL 67
           S+ L++F       A    +++ SCK A   DP + Y  CV+SL+ NP    A + + L+
Sbjct: 4   SLYLVMFFLLLNCFATAQSLIRDSCKKATTKDPKLKYDFCVKSLEENPQSKTAKSLEGLV 63

Query: 68  MISINHSKANATEIGSQISELLKGSNEKLGKYSLNCLKSCLELYSEAISDLEKALGALKM 127
            +S  ++ +  T +   + ++LK    ++ +  L+    CLELY+EAI  L ++L  +K 
Sbjct: 64  FVSTKNAVSKTTSLKGMVDKILKEDKYEVERPLLD----CLELYTEAIDSLNQSLDTVKS 123

Query: 128 EDYETANTGVSAAMDAPISCEDGY-REKDGEVSPFTEINDGFFQLIAISLAFINM 182
            DY+TA   +SAAMDAP SCE  + + K    SPFT+ ND  F ++ I +AF +M
Sbjct: 124 RDYKTATMLMSAAMDAPGSCETKFTKRKKAVKSPFTKENDVLFYMVLIPIAFTSM 174

[Close]

BLAST of Lsi05G000450 vs. TAIR 10
Match: AT5G46970.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 104.0 bits (258), Expect = 1.3e-22
Identity = 58/144 (40.28%), Postives = 87/144 (60.42%), Query Frame = 0


Query: 27  IVQQSCKLAAQTDPYVNYKLCVQSLKANPNYSKAANFKDLLMISINHSKANATEIGSQIS 86
           ++Q  CK AA  +P ++Y  CV+SL+ NP    A +   L+M+S  ++ +  T +   + 
Sbjct: 23  LIQDFCKKAADKNPKIHYNFCVKSLEENPQSKTARSLDRLVMLSTKNAVSKTTSMKGIVD 82

Query: 87  ELLKGSNEKLGKYSLNCLKSCLELYSEAISDLEKALGALKMEDYETANTGVSAAMDAPIS 146
           ++LK    +   YS   L+ CLELYS+A + L++AL  +K  DY+TAN  +SAAM AP S
Sbjct: 83  KILK--ENRFEMYSEKPLRDCLELYSDATNSLKEALTIIKSRDYKTANVVISAAMGAPPS 142

Query: 147 CEDGYRE-KDGEVSPFTEINDGFF 170
           CE G++E K    SPFT+ ND  F
Sbjct: 143 CEIGFKEGKKPLKSPFTKDNDVLF 164

[Close]

BLAST of Lsi05G000450 vs. TAIR 10
Match: AT5G46960.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 94.4 bits (233), Expect = 1.0e-19
Identity = 60/173 (34.68%), Postives = 91/173 (52.60%), Query Frame = 0


Query: 9   ISLLLFIPTFLQLAYCLDIVQQSCKLAAQTDPYVNYKLCVQSLKANPNYSKAANFKDLLM 68
           +SL++F       A    ++Q SCK A   DP ++Y  CV SL  +P    A   + L++
Sbjct: 5   VSLVIFSLFLNGFATAQTLIQDSCKKAFAKDPQLSYDFCVNSLTQDPQSKAATTLESLVL 64

Query: 69  ISINHSKANATEIGSQISELLKGSNEKLGKYSLNCLKSCLELYSEAISDLEKALGALKME 128
            S   + A  T +   +++ LK   ++  +  +  LK CL  Y++A  DL  AL  +K  
Sbjct: 65  ASTKTAAAKITNLKGIVAQDLK---DQRYQDIVEDLKLCLGFYNDANDDLTTALANIKSR 124

Query: 129 DYETANTGVSAAMDAPISCEDGYREKDGEVSPFTEINDGFFQLIAISLAFINM 182
           DY+ AN  +SAA+D P +CED ++E   + SP T  N   F+ I I LAF NM
Sbjct: 125 DYQGANINLSAALDVPGNCEDDFKEAK-KTSPITNENSILFKTILIPLAFTNM 173

[Close]

BLAST of Lsi05G000450 vs. TAIR 10
Match: AT5G46950.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 92.4 bits (228), Expect = 4.0e-19
Identity = 59/173 (34.10%), Postives = 92/173 (53.18%), Query Frame = 0


Query: 9   ISLLLFIPTFLQLAYCLDIVQQSCKLAAQTDPYVNYKLCVQSLKANPNYSKAANFKDLLM 68
           +SL++F       A    ++Q SCK A   DP  +Y  CVQSL  +P    A   +DL +
Sbjct: 5   VSLVIFSLFLNGFATAQTLIQDSCKKAFAKDPQSSYDFCVQSLTQDPQSKAATTLEDLAL 64

Query: 69  ISINHSKANATEIGSQISELLKGSNEKLGKYSLNCLKSCLELYSEAISDLEKALGALKME 128
            S  +  A  T +   +++ LK   ++  +  +  LK CL  Y +A   L+ AL  +K  
Sbjct: 65  ASTKNVAAKITNLKGIVAQDLK---DQRYQDIVEDLKLCLGFYKDANDSLKTALANIKSR 124

Query: 129 DYETANTGVSAAMDAPISCEDGYREKDGEVSPFTEINDGFFQLIAISLAFINM 182
           DY+ AN+ +SAA++AP  CED ++E + + SP T  N+  ++ I I LAF  M
Sbjct: 125 DYDGANSNLSAALNAPGDCEDDFKEAE-KKSPITNENNILYKTIVIPLAFTTM 173

The following BLAST results are available for this feature:

BLAST of Lsi05G000450 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8GT41	1.2e-23	40.45	Putative invertase inhibitor OS=Platanus acerifolia OX=140101 PE=1 SV=1	[more]
A9YUH4	7.5e-23	41.40	Putative invertase inhibitor OS=Platanus orientalis OX=122832 PE=1 SV=1	[more]
Q9FJR5	1.5e-18	34.68	Pectinesterase inhibitor 12 OS=Arabidopsis thaliana OX=3702 GN=PMEI12 PE=2 SV=1	[more]
Q9LUV1	2.9e-06	32.14	Pectinesterase inhibitor 2 OS=Arabidopsis thaliana OX=3702 GN=PMEI2 PE=1 SV=1	[more]

BLAST of Lsi05G000450 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LHE9	4.8e-73	80.11	PMEI domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G910710 PE=4 S...	[more]
A0A5A7TAE5	2.9e-70	77.90	Putative invertase inhibitor OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sca...	[more]
A0A1S3CQ13	2.9e-70	77.90	putative invertase inhibitor OS=Cucumis melo OX=3656 GN=LOC103503498 PE=4 SV=1	[more]
A0A6J1ICI2	5.0e-62	72.83	putative invertase inhibitor OS=Cucurbita maxima OX=3661 GN=LOC111471816 PE=4 SV...	[more]
A0A6J1H1W1	4.8e-57	67.40	putative invertase inhibitor OS=Cucurbita moschata OX=3662 GN=LOC111459677 PE=4 ...	[more]

BLAST of Lsi05G000450 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038888305.1	6.9e-74	80.33	putative invertase inhibitor [Benincasa hispida]	[more]
XP_004148520.1	1.0e-72	80.11	putative invertase inhibitor [Cucumis sativus]	[more]
XP_008465919.1	6.0e-70	77.90	PREDICTED: putative invertase inhibitor [Cucumis melo] >KAA0038527.1 putative in...	[more]
KAE8651458.1	1.4e-66	79.29	hypothetical protein Csa_001325 [Cucumis sativus]	[more]
XP_022973258.1	1.0e-61	72.83	putative invertase inhibitor [Cucurbita maxima]	[more]

BLAST of Lsi05G000450 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G46940.1	5.2e-27	37.99	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT5G46930.1	7.7e-23	34.86	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT5G46970.1	1.3e-22	40.28	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT5G46960.1	1.0e-19	34.68	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT5G46950.1	4.0e-19	34.10	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 108..128
None	No IPR available	PANTHER	PTHR31080:SF239	PLANT INVERTASE/PECTIN METHYLESTERASE INHIBITOR SUPERFAMILY PROTEIN	coord: 18..181
None	No IPR available	PANTHER	PTHR31080	PECTINESTERASE INHIBITOR-LIKE	coord: 18..181
IPR006501	Pectinesterase inhibitor domain	SMART	SM00856	PMEI_2	coord: 23..178 e-value: 9.9E-30 score: 114.8
IPR006501	Pectinesterase inhibitor domain	PFAM	PF04043	PMEI	coord: 27..177 e-value: 6.2E-30 score: 104.5
IPR006501	Pectinesterase inhibitor domain	TIGRFAM	TIGR01614	TIGR01614	coord: 1..181 e-value: 1.6E-36 score: 123.3
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	GENE3D	1.20.140.40	Invertase/pectin methylesterase inhibitor family protein	coord: 25..181 e-value: 2.0E-35 score: 124.0
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	SUPERFAMILY	101148	Plant invertase/pectin methylesterase inhibitor	coord: 26..181
IPR034088	Pla a 1-like	CDD	cd15795	PMEI-Pla_a_1_like	coord: 32..181 e-value: 1.54139E-44 score: 142.504

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi05G000450.1	Lsi05G000450.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0043086	negative regulation of catalytic activity
molecular_function	GO:0004857	enzyme inhibitor activity