Lsi04G022480 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi04G022480
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	Seryl-tRNA synthetase
Location	chr04: 29514918 .. 29518639 (+)
RNA-Seq Expression	Lsi04G022480
Synteny	Lsi04G022480

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

CATCAGAAGATGAAGATAATCGGGGCAAGGGCAGCCATCCGCTACTTCCATTCCTTCCCGCCAAGCAAAAACTTCCTCAAAGCTCCAACAATGGGATTCCAAACCCTTAAGTTCGCCGCCTTTCCAGCCTCTTCCACTTCCCGTCTAATTTTCCGGCCACCCACCAAAACCCCTTCTCTCAGTTCCCTTCATCCTTCTTTCCGTAGCCACAGACCTCCATCTCAATTGCTCGTTCGAGCTCTCTCATTGTCGGCTGTCCAAGTCGACCCTGCCACTGCAATCGCCGGTGAAGAAAAGGGTAATTTCATTTTCTCATCGTTGTTATTGTCGTTAACTTTTTTTTGATTCCTCCAATTTTCTTGGCGAGAAAATGGGTGTTTGATGTTGTGTATTGTTGTTCTTGATTTGGATTAGTTGTGAAACCTCAATGGAAAGCTTCAATAGACTTCAAGTGGATTAGGGATAACAAGGATTTGGTGGCTTCAAATATAGCGAATCGGAAATCTGGTGCTAATTTGGAACTTGTGCTCCAGCTCTATGACAAAATGTTAAATCTTCAGAAGGTTAGATATTGATTTGGTTTGTTTCCCCTTTTCTTTTCTGGGTGTTGACTTACTTATCATATGGATTAACTCTGTAATAATTCTTGCACCATTGTAGCTCTAACTGCATTGTTCATCAAAACTTTAGTTGCCCAACTATACACGAGGACAGACCATCAAACTAGTTCTTTTGCCCTTTTGCCCCTTTTTAAATCAATGCTTAGCTAACTGTTACTCACAGATACCAGGAAGTCGAGCGGCTTCGTGCGGAAAGGAATGTGGTTGCAAACAAGATGAAAGGGAAGCTGGAACCATCTGAGCGACAAAAACTCATAGAAGAAGGTATGTGCATTTTTCTGTCTGAAAATGTCGTTAGCTTCATTTTCAATTTCTGTGTTACCATATTTACTCTGGTGTTCTCTTTTCAGGAAAGAATCTGAAGGAAGGACTGGTTGCTTTGGAAGAAAACCTTCTTAAACTAACAGAACAGCTTCAACAAGAAGCACAATGTATACCAAACACGACCCATCCGGATGTTCCAGTCGGAGGGGAGGACTGCTCGATAATAAGAAAGATGGTATTTCTGCTTTTCAAACTCTGAATTATATTTCCTTACATCAATCCTTTTGAATGGTAGGCTTATGCCCTGCAATCTCTTTTGTAGGTCGGTAGCCCGAGGGAGTTCAGTTTTCCTGTTAAAGATCATCTTGAACTTGGGAAGCAGCTAAACCTTTTTGACTTTGATGCTGCTGCTGAGGTACAATCCTTGTTCAGTTTAAGGTACTTAAGAGCTGCTAGTTCATCTTGTTATGTCCATTTGAAAATGTTTGTGAAGAAGCTATTGTTTTGAGTCTTTTTAAGTCTTCACAGAGCACTTCTCCATCCTCCTTTTCTTTTTGTTAATGCTTATCATTCTATGTAATAAGATTGGAGGGCTGTATTTTTAAAAGATAAATAATTTAAACTTTTGAACTATCCAGTTCTTAGTTATGTTGTATGACATAATAAGTTAGATGTCGTCCTCGCATACGTTACTACGCTTATGAGTATTGGTTTAGCTGTTCATTGTCTCTATACACGACTTTGCCTCTTTTGTTTAGTTTGAGATATTCAATCCATTATCCTTAACTTGGCTTATTCTTATTTTAAACTTATATTAGTTTCCTTAACTTATGTTTTTGAATGCGCGCACGGCCTTAATGTTAAATTTTGCTGGTTTATACAGGTCAGTGGATCTAAGTTCTACTACCTGAAAAATGCGGCAGTAACATTAGAAATGGGTTTGATCAACTGGACACTCTCTGAAGTTATGAAGAGGGGCTTTACTCCTTTAATAACCCCAGAAATTGTTAGGTCCACAGTCGTTGAAAAGTGTGGATTCCAACCTCGTGGTGAAAATACTCAGGTTTTGCATATTTTTCTCGATTTTTTGCAGTATGATTGCTAGGTTCAATGTTGTGAGATAACTTTCCAGTATAAGATAATTTCATCCGTTCATAGCATGCACATTTGGTTTGACACAGCAGGCTAATTATAAGATTTCTTATACATATAGATGTGATTTAAATGTCATTTTAAGTCTCTGCTCTCCAGTCTAGACATACCCCAAGAAAAATGAGTGCCTTCTTTCCAACTATATTATTTGTAAGACGATTTAGTTACAATCCTTTCACACCCTTGGCTTGTTGGTTGGCAGGTTTATTCCATTGAGGGAAGTGACCAATGCCTCATTGGTACTGCAGAGATTCCTGTAGGAGGGATTCACATGGATTCCATCCTTTCTGAGTCTTCATTACCTTTGAAGTATGTTGCTTATTCTCATTGCTTTCGTACCGAGGCAGGTGCTGCTGGAGCTGCAACAAGGTACTGACCGATAAACTTCCCTGACCCTTTTCATTTTTCTCAATGACACTCTATTTTATATAACAAAGGCATGTTTGAGAGTGATTTTAAAGCGGTTAAAATAACTTTTGTCATGTTCACTGTTATTGAGACAACCCTAGGCATGCACCTAAGGCAAGAGGTGCTGTGACTTGAAGTTAGTGCACCTGTTGTCATGCCTAGGCGAACGTCTTCAAAGACCGAGCGCCTGCAAATATTTGCGTATTTTCTTTTAATAAAATTAATCGACAGCCCATGATGCCCACGTAAAGGCTTTTCGTGCAGAGATTTCGATTACTTACGTTTTTTTAACCCAATTTTTAAGACATGTTTCAAAGTGATTTCAAACATGATAAAAGTAATGTTAACAACTTCAACTTTACTCCCAAACATGTATTAAATCTCTTTCCTAAAATCAACATTTCTAAGGCTGTGATCCCACCGTCGTCTGAAAACTTCATCTTTTTCTAAATGAACAAGCCCTGAGCCTTAGCCTCACCTCTTTTGTTTCACTCACATCACTAATGTAGTCGCCTCTTCTCTAATGAGTATTTGATTTATTAATCTGATGATGAACAGAGGCCTGTATCGAGTTCACCAGTTCAGCAAGGTAGAAATGTTTATATTTTGCCGCCCTGAAGAGAGTGAGTCTTACCATGAAGAACTCATTAGGATTGAAGAGGACCTATTCTCATCACTGGGATTGCATTTCAAGTAATGATCTGCTTTTAGTTATCTTCATTGTCATTGTATTGTTTGAATAACACATCCGTTTTTGCCTTTTGGTTTCCCCATCTCACTTCGTTTCTGTTATCGTAGGACCTTGGATATGGCTTCAGCGGATTTAGGCGCACCTGCTTATCGAAAATTTGACGTGGAGGCATGGATGCCAGGTTTGGGACGATATGGCGAGGTACTCCATTCTCTCTCTCTCCTTTGTATGCAGTAAGGGTACAATTAAAGTTAAACTATTATGAGATGTTTGAATCTTATTTATAAATCATACGCTGTTGTCGTCAACAGATATCAAGTGCATCGAATTGTACAGACTATCAAAGCCGCAGATTAGGAATCCGATATCGTCCACAAGAACCCGTACCAACAACTTCAAAGAAGAGTAAAGGCAATCTGGCGCCTACACAATTTGTTCACACATTGAATGCGACAGCATGTGCTGTGCCTCGAATGATCGTTTGCTTGCTTGAAAATTATCAGCAGGAAGATGGATCTGTTATTATTCCTGAGCCATTGAGGCCTTTCATGGGTGGTCTAGAGCTAATTTCTCCTACATCTGCATAG

mRNA sequence

CATCAGAAGATGAAGATAATCGGGGCAAGGGCAGCCATCCGCTACTTCCATTCCTTCCCGCCAAGCAAAAACTTCCTCAAAGCTCCAACAATGGGATTCCAAACCCTTAAGTTCGCCGCCTTTCCAGCCTCTTCCACTTCCCGTCTAATTTTCCGGCCACCCACCAAAACCCCTTCTCTCAGTTCCCTTCATCCTTCTTTCCGTAGCCACAGACCTCCATCTCAATTGCTCGTTCGAGCTCTCTCATTGTCGGCTGTCCAAGTCGACCCTGCCACTGCAATCGCCGGTGAAGAAAAGGTTGTGAAACCTCAATGGAAAGCTTCAATAGACTTCAAGTGGATTAGGGATAACAAGGATTTGGTGGCTTCAAATATAGCGAATCGGAAATCTGGTGCTAATTTGGAACTTGTGCTCCAGCTCTATGACAAAATGTTAAATCTTCAGAAGGAAGTCGAGCGGCTTCGTGCGGAAAGGAATGTGGTTGCAAACAAGATGAAAGGGAAGCTGGAACCATCTGAGCGACAAAAACTCATAGAAGAAGGAAAGAATCTGAAGGAAGGACTGGTTGCTTTGGAAGAAAACCTTCTTAAACTAACAGAACAGCTTCAACAAGAAGCACAATGTATACCAAACACGACCCATCCGGATGTTCCAGTCGGAGGGGAGGACTGCTCGATAATAAGAAAGATGGTCGGTAGCCCGAGGGAGTTCAGTTTTCCTGTTAAAGATCATCTTGAACTTGGGAAGCAGCTAAACCTTTTTGACTTTGATGCTGCTGCTGAGGTACAATCCTTGTTCAGTTTAAGGTACTTAAGAGCTGCTAGTTCATCTTGTTATGTCCATTTGAAAATTCTTCACAGAGCACTTCTCCATCCTCCTTTTCTTTTTGTTAATGCTTATCATTCTATATGTCGTCCTCGCATACGTTACTACGCTTATGAGTATTGGTTTAGCTGTTCATTGTCTCTATACACGACTTTGCCTCTTTTGTTTATTTCCTTAACTTATGTTTTTGAATGCGCGCACGGCCTTAATGTCAGTGGATCTAAGTTCTACTACCTGAAAAATGCGGCAGTAACATTAGAAATGGGTTTGATCAACTGGACACTCTCTGAAGTTATGAAGAGGGGCTTTACTCCTTTAATAACCCCAGAAATTGTTAGGTCCACAGTCGTTGAAAAGTGTGGATTCCAACCTCGTGGTGAAAATACTCAGGTTTATTCCATTGAGGGAAGTGACCAATGCCTCATTGGTACTGCAGAGATTCCTGTAGGAGGGATTCACATGGATTCCATCCTTTCTGAGTCTTCATTACCTTTGAAGTATGTTGCTTATTCTCATTGCTTTCGTACCGAGGCAGGTGCTGCTGGAGCTGCAACAAGAGGCCTGTATCGAGTTCACCAGTTCAGCAAGGTAGAAATGTTTATATTTTGCCGCCCTGAAGAGAGTGAGTCTTACCATGAAGAACTCATTAGGATTGAAGAGGACCTATTCTCATCACTGGGATTGCATTTCAAGACCTTGGATATGGCTTCAGCGGATTTAGGCGCACCTGCTTATCGAAAATTTGACGTGGAGGCATGGATGCCAGGTTTGGGACGATATGGCGAGATATCAAGTGCATCGAATTGTACAGACTATCAAAGCCGCAGATTAGGAATCCGATATCGTCCACAAGAACCCGTACCAACAACTTCAAAGAAGAGTAAAGGCAATCTGGCGCCTACACAATTTGTTCACACATTGAATGCGACAGCATGTGCTGTGCCTCGAATGATCGTTTGCTTGCTTGAAAATTATCAGCAGGAAGATGGATCTGTTATTATTCCTGAGCCATTGAGGCCTTTCATGGGTGGTCTAGAGCTAATTTCTCCTACATCTGCATAG

Coding sequence (CDS)

ATGAAGATAATCGGGGCAAGGGCAGCCATCCGCTACTTCCATTCCTTCCCGCCAAGCAAAAACTTCCTCAAAGCTCCAACAATGGGATTCCAAACCCTTAAGTTCGCCGCCTTTCCAGCCTCTTCCACTTCCCGTCTAATTTTCCGGCCACCCACCAAAACCCCTTCTCTCAGTTCCCTTCATCCTTCTTTCCGTAGCCACAGACCTCCATCTCAATTGCTCGTTCGAGCTCTCTCATTGTCGGCTGTCCAAGTCGACCCTGCCACTGCAATCGCCGGTGAAGAAAAGGTTGTGAAACCTCAATGGAAAGCTTCAATAGACTTCAAGTGGATTAGGGATAACAAGGATTTGGTGGCTTCAAATATAGCGAATCGGAAATCTGGTGCTAATTTGGAACTTGTGCTCCAGCTCTATGACAAAATGTTAAATCTTCAGAAGGAAGTCGAGCGGCTTCGTGCGGAAAGGAATGTGGTTGCAAACAAGATGAAAGGGAAGCTGGAACCATCTGAGCGACAAAAACTCATAGAAGAAGGAAAGAATCTGAAGGAAGGACTGGTTGCTTTGGAAGAAAACCTTCTTAAACTAACAGAACAGCTTCAACAAGAAGCACAATGTATACCAAACACGACCCATCCGGATGTTCCAGTCGGAGGGGAGGACTGCTCGATAATAAGAAAGATGGTCGGTAGCCCGAGGGAGTTCAGTTTTCCTGTTAAAGATCATCTTGAACTTGGGAAGCAGCTAAACCTTTTTGACTTTGATGCTGCTGCTGAGGTACAATCCTTGTTCAGTTTAAGGTACTTAAGAGCTGCTAGTTCATCTTGTTATGTCCATTTGAAAATTCTTCACAGAGCACTTCTCCATCCTCCTTTTCTTTTTGTTAATGCTTATCATTCTATATGTCGTCCTCGCATACGTTACTACGCTTATGAGTATTGGTTTAGCTGTTCATTGTCTCTATACACGACTTTGCCTCTTTTGTTTATTTCCTTAACTTATGTTTTTGAATGCGCGCACGGCCTTAATGTCAGTGGATCTAAGTTCTACTACCTGAAAAATGCGGCAGTAACATTAGAAATGGGTTTGATCAACTGGACACTCTCTGAAGTTATGAAGAGGGGCTTTACTCCTTTAATAACCCCAGAAATTGTTAGGTCCACAGTCGTTGAAAAGTGTGGATTCCAACCTCGTGGTGAAAATACTCAGGTTTATTCCATTGAGGGAAGTGACCAATGCCTCATTGGTACTGCAGAGATTCCTGTAGGAGGGATTCACATGGATTCCATCCTTTCTGAGTCTTCATTACCTTTGAAGTATGTTGCTTATTCTCATTGCTTTCGTACCGAGGCAGGTGCTGCTGGAGCTGCAACAAGAGGCCTGTATCGAGTTCACCAGTTCAGCAAGGTAGAAATGTTTATATTTTGCCGCCCTGAAGAGAGTGAGTCTTACCATGAAGAACTCATTAGGATTGAAGAGGACCTATTCTCATCACTGGGATTGCATTTCAAGACCTTGGATATGGCTTCAGCGGATTTAGGCGCACCTGCTTATCGAAAATTTGACGTGGAGGCATGGATGCCAGGTTTGGGACGATATGGCGAGATATCAAGTGCATCGAATTGTACAGACTATCAAAGCCGCAGATTAGGAATCCGATATCGTCCACAAGAACCCGTACCAACAACTTCAAAGAAGAGTAAAGGCAATCTGGCGCCTACACAATTTGTTCACACATTGAATGCGACAGCATGTGCTGTGCCTCGAATGATCGTTTGCTTGCTTGAAAATTATCAGCAGGAAGATGGATCTGTTATTATTCCTGAGCCATTGAGGCCTTTCATGGGTGGTCTAGAGCTAATTTCTCCTACATCTGCATAG

Protein sequence

MKIIGARAAIRYFHSFPPSKNFLKAPTMGFQTLKFAAFPASSTSRLIFRPPTKTPSLSSLHPSFRSHRPPSQLLVRALSLSAVQVDPATAIAGEEKVVKPQWKASIDFKWIRDNKDLVASNIANRKSGANLELVLQLYDKMLNLQKEVERLRAERNVVANKMKGKLEPSERQKLIEEGKNLKEGLVALEENLLKLTEQLQQEAQCIPNTTHPDVPVGGEDCSIIRKMVGSPREFSFPVKDHLELGKQLNLFDFDAAAEVQSLFSLRYLRAASSSCYVHLKILHRALLHPPFLFVNAYHSICRPRIRYYAYEYWFSCSLSLYTTLPLLFISLTYVFECAHGLNVSGSKFYYLKNAAVTLEMGLINWTLSEVMKRGFTPLITPEIVRSTVVEKCGFQPRGENTQVYSIEGSDQCLIGTAEIPVGGIHMDSILSESSLPLKYVAYSHCFRTEAGAAGAATRGLYRVHQFSKVEMFIFCRPEESESYHEELIRIEEDLFSSLGLHFKTLDMASADLGAPAYRKFDVEAWMPGLGRYGEISSASNCTDYQSRRLGIRYRPQEPVPTTSKKSKGNLAPTQFVHTLNATACAVPRMIVCLLENYQQEDGSVIIPEPLRPFMGGLELISPTSA

Homology

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BLAST of Lsi04G022480 vs. ExPASy Swiss-Prot
Match: Q8RWT8 (Serine--tRNA ligase, chloroplastic/mitochondrial OS=Arabidopsis thaliana OX=3702 GN=OVA7 PE=2 SV=1)

HSP 1 Score: 724.2 bits (1868), Expect = 1.3e-207
Identity = 381/597 (63.82%), Postives = 433/597 (72.53%), Query Frame = 0


Query: 28  MGFQTLKF-AAFPASSTSRLIFRPPTKTPSLSSLHPSFRSHRPPSQLLVRALSLS-AVQV 87
           MG   L+  AA P + +SRL  +P T T +L       +    P  LLVRA S S AVQ 
Sbjct: 1   MGLHKLRLAAAVPVTFSSRLFLKPFTNTLTLGLFSRHLQPRNKP--LLVRAFSASAAVQD 60

Query: 88  DPATAIAGEEKVVKPQWKASIDFKWIRDNKDLVASNIANRKSGANLELVLQLYDKMLNLQ 147
            PAT  +      +P WKA+IDFKWIRDNK+ V  NI NR S ANLE VLQLY+ M+NLQ
Sbjct: 61  IPATQTSDSSVAARPLWKAAIDFKWIRDNKEAVEINIRNRNSNANLEAVLQLYENMVNLQ 120

Query: 148 KEVERLRAERNVVANKMKGKLEPSERQKLIEEGKNLKEGLVALEENLLKLTEQLQQEAQC 207
           KEVERLR ERN VA KMKGKLEPSER++L+EEGKNLKE LV LEE+L+KL ++LQ  AQ 
Sbjct: 121 KEVERLREERNNVAKKMKGKLEPSERERLVEEGKNLKESLVTLEEDLVKLKDELQHVAQS 180

Query: 208 IPNTTHPDVPVGGEDCSIIRKMVGSPREFSFPVKDHLELGKQLNLFDFDAAAEVQSLFSL 267
           IPN THPDVPVGGED S IR+ VGSPREFSFP+KDHL+LGK L+L DFD+AAE       
Sbjct: 181 IPNMTHPDVPVGGEDSSAIRQEVGSPREFSFPIKDHLQLGKDLDLIDFDSAAE------- 240

Query: 268 RYLRAASSSCYVHLKILHRALLHPPFLFVNAYHSICRPRIRYYAYEYWFSCSLSLYTTLP 327
                                                                       
Sbjct: 241 ------------------------------------------------------------ 300

Query: 328 LLFISLTYVFECAHGLNVSGSKFYYLKNAAVTLEMGLINWTLSEVMKRGFTPLITPEIVR 387
                            VSGSKF+YLKN AV LEM L+NWTLS+VMK+G+TPL TPEIVR
Sbjct: 301 -----------------VSGSKFFYLKNEAVLLEMALLNWTLSQVMKKGYTPLTTPEIVR 360

Query: 388 STVVEKCGFQPRGENTQVYSIEGSDQCLIGTAEIPVGGIHMDSILSESSLPLKYVAYSHC 447
           S++VEKCGFQPRG+NTQVYSI+G+DQCLIGTAEIPVGGIHMDSIL ES+LPLKY+A+SHC
Sbjct: 361 SSIVEKCGFQPRGDNTQVYSIDGTDQCLIGTAEIPVGGIHMDSILLESALPLKYIAFSHC 420

Query: 448 FRTEAGAAGAATRGLYRVHQFSKVEMFIFCRPEESESYHEELIRIEEDLFSSLGLHFKTL 507
           FRTEAGAAGAAT+GLYRVHQFSK EMF+ C+PE+SES+H+ELI+IEEDLF+SLGLHFKTL
Sbjct: 421 FRTEAGAAGAATKGLYRVHQFSKAEMFVICQPEDSESFHQELIQIEEDLFTSLGLHFKTL 480

Query: 508 DMASADLGAPAYRKFDVEAWMPGLGRYGEISSASNCTDYQSRRLGIRYRPQEPVPTTSKK 567
           DMA+ADLGAPAYRKFD+EAWMPGLGR+GEISSASNCTDYQSRRLGIRYRP EP  T  KK
Sbjct: 481 DMATADLGAPAYRKFDIEAWMPGLGRFGEISSASNCTDYQSRRLGIRYRPSEPPQTGPKK 511

Query: 568 SKGNLAPTQFVHTLNATACAVPRMIVCLLENYQQEDGSVIIPEPLRPFMGGLELISP 623
            K NL  T+FVHTLNATACAVPRM+VCLLENYQQEDGSV+IPEPLRPFMGG+ELI P
Sbjct: 541 GKANLPATKFVHTLNATACAVPRMMVCLLENYQQEDGSVVIPEPLRPFMGGIELIKP 511

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BLAST of Lsi04G022480 vs. ExPASy Swiss-Prot
Match: Q73KB2 (Serine--tRNA ligase OS=Treponema denticola (strain ATCC 35405 / DSM 14222 / CIP 103919 / JCM 8153 / KCTC 15104) OX=243275 GN=serS PE=3 SV=1)

HSP 1 Score: 387.1 bits (993), Expect = 3.7e-106
Identity = 209/513 (40.74%), Postives = 286/513 (55.75%), Query Frame = 0


Query: 106 IDFKWIRDNKDLVASNIANRKSGANLELVLQLYDKMLNLQKEVERLRAERNVVANKMKGK 165
           +D+K+I++N + V  NI NR   A+ +  ++LYDK   L   ++ L+ +RN  +  MK K
Sbjct: 2   LDYKFIKENVEAVKQNIKNRHMNADADKAVELYDKRTALVTSLQNLQKDRNDNSQSMKQK 61

Query: 166 LEPSERQKLIEEGKNLKEGLVALEENLLKLTEQLQQEAQCIPNTTHPDVPVGGEDCSIIR 225
           L P ERQKL+++GK +KE +  +E  L +  + L +    IPN  HP+ PVG ED   + 
Sbjct: 62  LSPEERQKLVDQGKAIKEKIAQVEAELAEAEKALHEAVSKIPNMAHPEAPVGKEDSDNLE 121

Query: 226 -KMVGSPREFSFPVKDHLELGKQLNLFDFDAAAEVQSLFSLRYLRAASSSCYVHLKILHR 285
            K  G+  +F F  KDH++LG+ L+L DF+A                             
Sbjct: 122 VKRCGTVPKFDFEPKDHVQLGQDLDLIDFEA----------------------------- 181

Query: 286 ALLHPPFLFVNAYHSICRPRIRYYAYEYWFSCSLSLYTTLPLLFISLTYVFECAHGLNVS 345
                                                                  G  VS
Sbjct: 182 -------------------------------------------------------GTKVS 241

Query: 346 GSKFYYLKNAAVTLEMGLINWTLSEVMKRGFTPLITPEIVRSTVVEKCGFQPRGENTQVY 405
           G KFY+LKN AV LE  L  + L+ + K GF P ITP+I +  ++   GF PRGE + VY
Sbjct: 242 GVKFYFLKNEAVFLEQALTMYGLNILRKHGFKPFITPDIAKEEILYGIGFNPRGEESNVY 301

Query: 406 SIEGSDQCLIGTAEIPVGGIHMDSILSESSLPLKYVAYSHCFRTEAGAAGAATRGLYRVH 465
           S+EG   CL+ TAEI +GG H D I+ + SLPLKY   SHCFR EAGAAG  ++GLYRVH
Sbjct: 302 SLEGEGTCLVATAEITLGGYHSDEIIKKESLPLKYCGLSHCFRREAGAAGQFSKGLYRVH 361

Query: 466 QFSKVEMFIFCRPEESESYHEELIRIEEDLFSSLGLHFKTLDMASADLGAPAYRKFDVEA 525
           QFSK+EMF++C PEES++ HEEL  IEE++F+ LG+ F+ +D  + DLGAPAYRK+D+EA
Sbjct: 362 QFSKLEMFVYCTPEESDALHEELRLIEEEIFNGLGIPFRVVDTCTGDLGAPAYRKWDLEA 417

Query: 526 WMPGL--GRYGEISSASNCTDYQSRRLGIRYRPQEPVPTTSKKSKGNLAPTQFVHTLNAT 585
           WMPG   G +GE++S SNCTDYQ+RRL IRY+  +                +F+HTLN T
Sbjct: 422 WMPGRNGGEWGEVTSTSNCTDYQARRLNIRYKDDD-------------GKNKFLHTLNGT 417

Query: 586 ACAVPRMIVCLLENYQQEDGSVIIPEPLRPFMG 616
           A A+ R ++ +LENYQQ DGS+ IPE L P+ G
Sbjct: 482 ALAMSRAMIAVLENYQQADGSIKIPEALVPYCG 417

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BLAST of Lsi04G022480 vs. ExPASy Swiss-Prot
Match: Q1AX26 (Serine--tRNA ligase OS=Rubrobacter xylanophilus (strain DSM 9941 / NBRC 16129 / PRD-1) OX=266117 GN=serS PE=3 SV=1)

HSP 1 Score: 358.6 bits (919), Expect = 1.4e-97
Identity = 204/518 (39.38%), Postives = 287/518 (55.41%), Query Frame = 0


Query: 106 IDFKWIRDNKDLVASNIANRKSGANLELVLQLYDKMLNLQKEVERLRAERNVVANKMKGK 165
           +D ++IR+N + V  N  +R   A+++LV++L D+   L +E+E LRA +N +A  +  +
Sbjct: 2   LDLRYIRENAEAVKKNCRDRGVEADVDLVVELADRRSALIRELEGLRARQNQLAKAVAKE 61

Query: 166 LEPSERQKLIEEGKNLKEGLVALEENLLKLTEQLQQEAQCIPNTTHPDVPVGGEDCSIIR 225
            +   R +LIEE + LK  +   EE L  + E+L +E   IPN THP+ PVG +D   + 
Sbjct: 62  RDERSRGRLIEESRELKGLIPRREEELRGVEERLAEEQLKIPNMTHPEAPVGRDDSENVE 121

Query: 226 -KMVGSPREFSFPVKDHLELGKQLNLFDFDAAAEVQSLFSLRYLRAASSSCYVHLKILHR 285
            +  G   EF F  KDH+ELG+ L + DFDA                             
Sbjct: 122 IRRWGEAPEFGFEPKDHVELGEALGIIDFDA----------------------------- 181

Query: 286 ALLHPPFLFVNAYHSICRPRIRYYAYEYWFSCSLSLYTTLPLLFISLTYVFECAHGLNVS 345
                                                                  G   +
Sbjct: 182 -------------------------------------------------------GAKTT 241

Query: 346 GSKFYYLKNAAVTLEMGLINWTLSEVMKRGFTPLITPEIVRSTVVEKCGFQPRGENTQVY 405
           GSKFY+L+  AV LE+GL+ + L  +  RG+   +TP++ R  ++   GF PRG  TQ+Y
Sbjct: 242 GSKFYFLRGEAVLLELGLVRFALDLLAGRGYELAMTPDLARDRMLLGTGFVPRGPETQIY 301

Query: 406 SIEGSDQCLIGTAEIPVGGIHMDSILSESSLPLKYVAYSHCFRTEAGAAGAATRGLYRVH 465
           S+E +D  LI TAEI + G  +D I++E SLP ++   SHCFRTEAGA G A+RGLYRVH
Sbjct: 302 SVENTDLSLIATAEITLAGQLVDEIVAEESLPRRFAGLSHCFRTEAGAHGRASRGLYRVH 361

Query: 466 QFSKVEMFIFCRPEESESYHEELIRIEEDLFSSLGLHFKTLDMASADLGAPAYRKFDVEA 525
           QF+KVEMF F  PE+S   HEE++ IEE++F +L + ++ +D+ + DLGA AYRK+DVEA
Sbjct: 362 QFTKVEMFAFTTPEQSGEMHEEMLSIEEEIFRTLEIPYRVVDICTGDLGAAAYRKYDVEA 421

Query: 526 WMPGLGRYGEISSASNCTDYQSRRLGIRYRPQEPVPTTSKKSKGNLAPTQFVHTLNATAC 585
           WMPG G YGE++S SN TDYQ+RRLGIRYRP+   P             + +HTLN TA 
Sbjct: 422 WMPGRGDYGEVTSTSNTTDYQARRLGIRYRPEGGRP-------------RLLHTLNGTAI 421

Query: 586 AVPRMIVCLLENYQQEDGSVIIPEPLRPFMGGLELISP 623
           AV R ++ LLEN+Q+EDGSV +PE L P++ G E+I P
Sbjct: 482 AVSRTMIALLENHQREDGSVALPEALVPYV-GREVIGP 421

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BLAST of Lsi04G022480 vs. ExPASy Swiss-Prot
Match: B5RL50 (Serine--tRNA ligase OS=Borrelia duttonii (strain Ly) OX=412419 GN=serS PE=3 SV=1)

HSP 1 Score: 356.3 bits (913), Expect = 7.1e-97
Identity = 209/521 (40.12%), Postives = 285/521 (54.70%), Query Frame = 0


Query: 106 IDFKWIRDNKDLVASNIANRKSGANLELVLQLYDKMLNLQKEVERLRAERNVVANKMKGK 165
           +D K+IRDN +LV  NI NR     L+L++   D+   L  ++  L A RN  AN MK  
Sbjct: 2   LDLKFIRDNLELVRENIKNRGLKLELDLLISFDDERRKLITKIGELNALRNENANAMKDN 61

Query: 166 LEPSERQKLIEEGKNLKEGLVALEENLLKLTEQLQQEAQCIPNTTHPDVPVG-GEDCSII 225
           ++ S R  LIE+GK LK  ++ LEE L  LT +L  E + IPN + PDVPVG  ED +++
Sbjct: 62  IDDSSRHYLIEKGKALKAEIMDLEERLGYLTSRLLVEHKRIPNISAPDVPVGVSEDENVV 121

Query: 226 RKMVGSPREFSFPVKDHLELGKQLNLFDFDAAAEVQSLFSLRYLRAASSSCYVHLKILHR 285
            K+ G+  +F F  KDHLE+G  L+LFDF+ A E                          
Sbjct: 122 LKVSGNIPKFDFKPKDHLEIGIHLDLFDFEKARE-------------------------- 181

Query: 286 ALLHPPFLFVNAYHSICRPRIRYYAYEYWFSCSLSLYTTLPLLFISLTYVFECAHGLNVS 345
                                                                     VS
Sbjct: 182 ----------------------------------------------------------VS 241

Query: 346 GSKFYYLKNAAVTLEMGLINWTLSEVMKRGFTPLITPEIVRSTVVEKCGFQPRGENTQVY 405
           G+KFYYLKN AV LE+ +IN+  S++  +GF   ITP++ R  +V+  GF PRG  + +Y
Sbjct: 242 GNKFYYLKNEAVFLELAMINFAFSKLKAKGFDLFITPDVAREFIVDGIGFNPRGAESNIY 301

Query: 406 SIEGSDQCLIGTAEIPVGGIHMDSILSESSLPLKYVAYSHCFRTEAGAAGAATRGLYRVH 465
            IE +D+ L+GTAEI +GG + ++IL   S PLK    SHCFR EAGAAG  ++GLYRVH
Sbjct: 302 KIEDTDKYLVGTAEITLGGYYYNTILDLKS-PLKMAGLSHCFRKEAGAAGQFSKGLYRVH 361

Query: 466 QFSKVEMFIFCRPEESESYHEELIRIEEDLFSSLGLHFKTLDMASADLGAPAYRKFDVEA 525
           QFSKVEMF FCR E+S+  H E + +EE++F+ L + ++ L++ S+DLGAPAY+K+D+EA
Sbjct: 362 QFSKVEMFCFCRSEDSDRIHNEFLELEEEIFTELEIPYRVLNVCSSDLGAPAYKKYDIEA 421

Query: 526 WMPGLGR---YGEISSASNCTDYQSRRLGIRYRPQEPVPTTSKKSKGNLAPTQFVHTLNA 585
           WMPG G    YGE++S SNCTDYQSRRL IRY+                   +FVH +N 
Sbjct: 422 WMPGRGENGDYGEVTSTSNCTDYQSRRLKIRYKED--------------GQNKFVHMING 422

Query: 586 TACAVPRMIVCLLENYQQEDGSVIIPEPLRPFMGGLELISP 623
           TA A  R ++ +LEN+Q   G V IP+ L  +  G + ISP
Sbjct: 482 TAIASTRTLIAILENFQDAKGGVRIPKSLVKYT-GFDYISP 422

[Close]

BLAST of Lsi04G022480 vs. ExPASy Swiss-Prot
Match: B5RR43 (Serine--tRNA ligase OS=Borrelia recurrentis (strain A1) OX=412418 GN=serS PE=3 SV=1)

HSP 1 Score: 353.6 bits (906), Expect = 4.6e-96
Identity = 209/521 (40.12%), Postives = 283/521 (54.32%), Query Frame = 0


Query: 106 IDFKWIRDNKDLVASNIANRKSGANLELVLQLYDKMLNLQKEVERLRAERNVVANKMKGK 165
           +D K+IRDN +LV  NI NR     L+L++   D+   L  ++  L A RN  AN MK  
Sbjct: 2   LDLKFIRDNLELVRENIKNRGLKLELDLLISFDDERRKLITKIGELNALRNENANAMKDN 61

Query: 166 LEPSERQKLIEEGKNLKEGLVALEENLLKLTEQLQQEAQCIPNTTHPDVPVG-GEDCSII 225
           +  S R  LIE+GK LK  ++ LEE L  LT +L  E + IPN + PDVPVG  ED +++
Sbjct: 62  IGDSSRHYLIEKGKALKAEIMDLEERLGYLTSRLLVEHKRIPNISAPDVPVGVSEDENVV 121

Query: 226 RKMVGSPREFSFPVKDHLELGKQLNLFDFDAAAEVQSLFSLRYLRAASSSCYVHLKILHR 285
            K+ G+  +F F  KDHLE+G  L+LFDF+ A E                          
Sbjct: 122 LKVSGNIPKFDFKPKDHLEIGIHLDLFDFEKARE-------------------------- 181

Query: 286 ALLHPPFLFVNAYHSICRPRIRYYAYEYWFSCSLSLYTTLPLLFISLTYVFECAHGLNVS 345
                                                                     VS
Sbjct: 182 ----------------------------------------------------------VS 241

Query: 346 GSKFYYLKNAAVTLEMGLINWTLSEVMKRGFTPLITPEIVRSTVVEKCGFQPRGENTQVY 405
           G+KFYYLKN AV LE+ +IN+  S++  +GF   ITP++ R  +V+  GF PRG  + +Y
Sbjct: 242 GNKFYYLKNEAVFLELAMINFAFSKLKAKGFDLFITPDVAREFIVDGIGFNPRGAESNIY 301

Query: 406 SIEGSDQCLIGTAEIPVGGIHMDSILSESSLPLKYVAYSHCFRTEAGAAGAATRGLYRVH 465
            IE +D+ L+GTAEI +GG + ++IL   S PLK    SHCFR EAGAAG  ++GLYRVH
Sbjct: 302 KIEDTDKYLVGTAEITLGGYYYNTILDLKS-PLKMAGLSHCFRKEAGAAGQFSKGLYRVH 361

Query: 466 QFSKVEMFIFCRPEESESYHEELIRIEEDLFSSLGLHFKTLDMASADLGAPAYRKFDVEA 525
           QFSKVEMF FCR E+S+  H E + +EE++F  L + ++ L++ S+DLGAPAY+K+D+EA
Sbjct: 362 QFSKVEMFCFCRSEDSDRIHNEFLELEEEIFIELEIPYRVLNVCSSDLGAPAYKKYDIEA 421

Query: 526 WMPGLGR---YGEISSASNCTDYQSRRLGIRYRPQEPVPTTSKKSKGNLAPTQFVHTLNA 585
           WMPG G    YGE++S SNCTDYQSRRL IRY+                   +FVH +N 
Sbjct: 422 WMPGRGENGDYGEVTSTSNCTDYQSRRLKIRYKED--------------GQNKFVHMING 422

Query: 586 TACAVPRMIVCLLENYQQEDGSVIIPEPLRPFMGGLELISP 623
           TA A  R ++ +LEN+Q   G V IP+ L  +  G + ISP
Sbjct: 482 TAIASTRTLIAILENFQDAKGGVRIPKSLVKYT-GFDYISP 422

[Close]

BLAST of Lsi04G022480 vs. ExPASy TrEMBL
Match: A0A0A0LHK3 (Seryl-tRNA synthetase OS=Cucumis sativus OX=3659 GN=Csa_3G889830 PE=4 SV=1)

HSP 1 Score: 952.6 bits (2461), Expect = 8.2e-274
Identity = 496/624 (79.49%), Postives = 516/624 (82.69%), Query Frame = 0


Query: 3   IIGARAAIRYFHSFPPSKNFLKAPTMGFQTLKFAAFPASSTSRLIFRPPTKTPSLSSLHP 62
           I+G R AIR+FHSFPPS  F KA TMGFQTLK  AFPASSTSRL+  PPTKTPSL+SLHP
Sbjct: 5   IMGERGAIRHFHSFPPSHTFPKASTMGFQTLKLPAFPASSTSRLMVLPPTKTPSLTSLHP 64

Query: 63  SFRSHRPPSQLLVRALSLSAVQVD-PATAIAGEEKVVKPQWKASIDFKWIRDNKDLVASN 122
             R+HRPPS+LLVRA SL +V+ D P + IAGEEKVVKPQWKASIDFKWIRDNKDLVASN
Sbjct: 65  CVRTHRPPSRLLVRAFSLPSVEFDHPPSTIAGEEKVVKPQWKASIDFKWIRDNKDLVASN 124

Query: 123 IANRKSGANLELVLQLYDKMLNLQKEVERLRAERNVVANKMKGKLEPSERQKLIEEGKNL 182
           I NRKSGANLELVLQLYDKMLNLQKEVE+LRAERN+VANKMKGKLEPSERQKLIEEGKNL
Sbjct: 125 IQNRKSGANLELVLQLYDKMLNLQKEVEQLRAERNMVANKMKGKLEPSERQKLIEEGKNL 184

Query: 183 KEGLVALEENLLKLTEQLQQEAQCIPNTTHPDVPVGGEDCSIIRKMVGSPREFSFPVKDH 242
           KEGLV+LEENLLKLT+QLQQEAQCIPNTTHPDVP+GGEDCSIIRKMVGSPREFSFPVKDH
Sbjct: 185 KEGLVSLEENLLKLTDQLQQEAQCIPNTTHPDVPIGGEDCSIIRKMVGSPREFSFPVKDH 244

Query: 243 LELGKQLNLFDFDAAAEVQSLFSLRYLRAASSSCYVHLKILHRALLHPPFLFVNAYHSIC 302
           LELGKQL++FDFDAAAE                                           
Sbjct: 245 LELGKQLDIFDFDAAAE------------------------------------------- 304

Query: 303 RPRIRYYAYEYWFSCSLSLYTTLPLLFISLTYVFECAHGLNVSGSKFYYLKNAAVTLEMG 362
                                                    VSGSKFYYLKNAAVTLE+G
Sbjct: 305 -----------------------------------------VSGSKFYYLKNAAVTLELG 364

Query: 363 LINWTLSEVMKRGFTPLITPEIVRSTVVEKCGFQPRGENTQVYSIEGSDQCLIGTAEIPV 422
           LINWTLSEVMKRGFTPLITPEIVRSTVVEKCGFQPRGENTQVYSIEGSDQCLIGTAEIPV
Sbjct: 365 LINWTLSEVMKRGFTPLITPEIVRSTVVEKCGFQPRGENTQVYSIEGSDQCLIGTAEIPV 424

Query: 423 GGIHMDSILSESSLPLKYVAYSHCFRTEAGAAGAATRGLYRVHQFSKVEMFIFCRPEESE 482
           GGIHMDSILSESSLPLKYVAYSHCFRTEAGAAGAATRGLYRVHQFSKVEMFIFCRPEESE
Sbjct: 425 GGIHMDSILSESSLPLKYVAYSHCFRTEAGAAGAATRGLYRVHQFSKVEMFIFCRPEESE 484

Query: 483 SYHEELIRIEEDLFSSLGLHFKTLDMASADLGAPAYRKFDVEAWMPGLGRYGEISSASNC 542
           SYHEELIRIEEDLFSSLGLHFKTLDMASADLGAPAYRKFDVEAWMPGLGRYGEISSASNC
Sbjct: 485 SYHEELIRIEEDLFSSLGLHFKTLDMASADLGAPAYRKFDVEAWMPGLGRYGEISSASNC 544

Query: 543 TDYQSRRLGIRYRPQEPVPTTSKKSKGNLAPTQFVHTLNATACAVPRMIVCLLENYQQED 602
           TDYQSRRLGIRYRPQE VP+T KK KGNLAPTQFVHTLNATACAVPRM+VCLLENYQQED
Sbjct: 545 TDYQSRRLGIRYRPQEQVPSTLKKGKGNLAPTQFVHTLNATACAVPRMMVCLLENYQQED 544

Query: 603 GSVIIPEPLRPFMGGLELISPTSA 626
           GSVIIPEPLRPFMGGL+ ISP SA
Sbjct: 605 GSVIIPEPLRPFMGGLDRISPASA 544

[Close]

BLAST of Lsi04G022480 vs. ExPASy TrEMBL
Match: A0A1S3CQP4 (Seryl-tRNA synthetase OS=Cucumis melo OX=3656 GN=LOC103503692 PE=4 SV=1)

HSP 1 Score: 932.2 bits (2408), Expect = 1.2e-267
Identity = 486/603 (80.60%), Postives = 502/603 (83.25%), Query Frame = 0


Query: 24  KAPTMGFQTLKFAAFPASSTSRLIFRPPTKTPSLSSLHPSFRSHRPPSQLLVRALSLSAV 83
           KA TMGFQTLK A FPASSTSRL+F PPTKTPSL+  HP  R+HRPPS+L VRA SL +V
Sbjct: 43  KASTMGFQTLKLADFPASSTSRLMFLPPTKTPSLTPFHPCVRTHRPPSRLFVRAFSLPSV 102

Query: 84  QVD-PATAIAGEEKVVKPQWKASIDFKWIRDNKDLVASNIANRKSGANLELVLQLYDKML 143
           QVD P T IAGEEKVVKPQWKASIDFKWIRDNKDLVASNI NRKSGANLELVLQLYD+ML
Sbjct: 103 QVDHPPTTIAGEEKVVKPQWKASIDFKWIRDNKDLVASNIHNRKSGANLELVLQLYDEML 162

Query: 144 NLQKEVERLRAERNVVANKMKGKLEPSERQKLIEEGKNLKEGLVALEENLLKLTEQLQQE 203
           NLQKEVE+LRAERNVVANKMKGKLEPSERQKLIEEGKNLKEGLV+LEENLLKLT+QLQQE
Sbjct: 163 NLQKEVEQLRAERNVVANKMKGKLEPSERQKLIEEGKNLKEGLVSLEENLLKLTDQLQQE 222

Query: 204 AQCIPNTTHPDVPVGGEDCSIIRKMVGSPREFSFPVKDHLELGKQLNLFDFDAAAEVQSL 263
           AQCIPNTTHPDVP+GGEDCSIIRKMVGSPREFSFPVKDHLELG+QL++FDFDAAAE    
Sbjct: 223 AQCIPNTTHPDVPIGGEDCSIIRKMVGSPREFSFPVKDHLELGRQLDIFDFDAAAE---- 282

Query: 264 FSLRYLRAASSSCYVHLKILHRALLHPPFLFVNAYHSICRPRIRYYAYEYWFSCSLSLYT 323
                                                                       
Sbjct: 283 ------------------------------------------------------------ 342

Query: 324 TLPLLFISLTYVFECAHGLNVSGSKFYYLKNAAVTLEMGLINWTLSEVMKRGFTPLITPE 383
                               VSGSKFYYLKNAAVTLE+GLINWTLSEVMKRGFTPLITPE
Sbjct: 343 --------------------VSGSKFYYLKNAAVTLELGLINWTLSEVMKRGFTPLITPE 402

Query: 384 IVRSTVVEKCGFQPRGENTQVYSIEGSDQCLIGTAEIPVGGIHMDSILSESSLPLKYVAY 443
           IVRSTVVEKCGFQPRGENTQVYSIEGSDQCLIGTAEIPVGGIHMDSILSESSLPLKYVAY
Sbjct: 403 IVRSTVVEKCGFQPRGENTQVYSIEGSDQCLIGTAEIPVGGIHMDSILSESSLPLKYVAY 462

Query: 444 SHCFRTEAGAAGAATRGLYRVHQFSKVEMFIFCRPEESESYHEELIRIEEDLFSSLGLHF 503
           SHCFRTEAGAAGAATRGLYRVHQFSKVEMFIFCRPEESESYHEELIRIEEDLFSSLGLHF
Sbjct: 463 SHCFRTEAGAAGAATRGLYRVHQFSKVEMFIFCRPEESESYHEELIRIEEDLFSSLGLHF 522

Query: 504 KTLDMASADLGAPAYRKFDVEAWMPGLGRYGEISSASNCTDYQSRRLGIRYRPQEPVPTT 563
           KTLDMASADLGAPAYRKFDVEAWMPGLGRYGEISSASNCTDYQSRRLGIRYRPQEPVP+T
Sbjct: 523 KTLDMASADLGAPAYRKFDVEAWMPGLGRYGEISSASNCTDYQSRRLGIRYRPQEPVPST 561

Query: 564 SKKSKGNLAPTQFVHTLNATACAVPRMIVCLLENYQQEDGSVIIPEPLRPFMGGLELISP 623
            KK KGNLAPTQFVHTLNATACAVPRMIVCLLENYQQEDGSVIIPEPLRPFMGGL+LISP
Sbjct: 583 LKKGKGNLAPTQFVHTLNATACAVPRMIVCLLENYQQEDGSVIIPEPLRPFMGGLDLISP 561

Query: 624 TSA 626
           TSA
Sbjct: 643 TSA 561

[Close]

BLAST of Lsi04G022480 vs. ExPASy TrEMBL
Match: A0A5A7TBE3 (Seryl-tRNA synthetase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold455G006050 PE=4 SV=1)

HSP 1 Score: 927.5 bits (2396), Expect = 2.8e-266
Identity = 483/599 (80.63%), Postives = 499/599 (83.31%), Query Frame = 0


Query: 28  MGFQTLKFAAFPASSTSRLIFRPPTKTPSLSSLHPSFRSHRPPSQLLVRALSLSAVQVD- 87
           MGFQTLK A FPASSTSRL+F PPTKTPSL+  HP  R+HRPPS+L VRA SL +VQVD 
Sbjct: 1   MGFQTLKLADFPASSTSRLMFLPPTKTPSLTPFHPCVRTHRPPSRLFVRAFSLPSVQVDH 60

Query: 88  PATAIAGEEKVVKPQWKASIDFKWIRDNKDLVASNIANRKSGANLELVLQLYDKMLNLQK 147
           P T IAGEEKVVKPQWKASIDFKWIRDNKDLVASNI NRKSGANLELVLQLYD+MLNLQK
Sbjct: 61  PPTTIAGEEKVVKPQWKASIDFKWIRDNKDLVASNIHNRKSGANLELVLQLYDEMLNLQK 120

Query: 148 EVERLRAERNVVANKMKGKLEPSERQKLIEEGKNLKEGLVALEENLLKLTEQLQQEAQCI 207
           EVE+LRAERNVVANKMKGKLEPSERQKLIEEGKNLKEGLV+LEENLLKLT+QLQQEAQCI
Sbjct: 121 EVEQLRAERNVVANKMKGKLEPSERQKLIEEGKNLKEGLVSLEENLLKLTDQLQQEAQCI 180

Query: 208 PNTTHPDVPVGGEDCSIIRKMVGSPREFSFPVKDHLELGKQLNLFDFDAAAEVQSLFSLR 267
           PNTTHPDVP+GGEDCSIIRKMVGSPREFSFPVKDHLELG+QL++FDFDAAAE        
Sbjct: 181 PNTTHPDVPIGGEDCSIIRKMVGSPREFSFPVKDHLELGRQLDIFDFDAAAE-------- 240

Query: 268 YLRAASSSCYVHLKILHRALLHPPFLFVNAYHSICRPRIRYYAYEYWFSCSLSLYTTLPL 327
                                                                       
Sbjct: 241 ------------------------------------------------------------ 300

Query: 328 LFISLTYVFECAHGLNVSGSKFYYLKNAAVTLEMGLINWTLSEVMKRGFTPLITPEIVRS 387
                           VSGSKFYYLKNAAVTLE+GLINWTLSEVMKRGFTPLITPEIVRS
Sbjct: 301 ----------------VSGSKFYYLKNAAVTLELGLINWTLSEVMKRGFTPLITPEIVRS 360

Query: 388 TVVEKCGFQPRGENTQVYSIEGSDQCLIGTAEIPVGGIHMDSILSESSLPLKYVAYSHCF 447
           TVVEKCGFQPRGENTQVYSIEGSDQCLIGTAEIPVGGIHMDSILSESSLPLKYVAYSHCF
Sbjct: 361 TVVEKCGFQPRGENTQVYSIEGSDQCLIGTAEIPVGGIHMDSILSESSLPLKYVAYSHCF 420

Query: 448 RTEAGAAGAATRGLYRVHQFSKVEMFIFCRPEESESYHEELIRIEEDLFSSLGLHFKTLD 507
           RTEAGAAGAATRGLYRVHQFSKVEMFIFCRPEESESYHEELIRIEEDLFSSLGLHFKTLD
Sbjct: 421 RTEAGAAGAATRGLYRVHQFSKVEMFIFCRPEESESYHEELIRIEEDLFSSLGLHFKTLD 480

Query: 508 MASADLGAPAYRKFDVEAWMPGLGRYGEISSASNCTDYQSRRLGIRYRPQEPVPTTSKKS 567
           MASADLGAPAYRKFDVEAWMPGLGRYGEISSASNCTDYQSRRLGIRYRPQEPVP+T KK 
Sbjct: 481 MASADLGAPAYRKFDVEAWMPGLGRYGEISSASNCTDYQSRRLGIRYRPQEPVPSTLKKG 515

Query: 568 KGNLAPTQFVHTLNATACAVPRMIVCLLENYQQEDGSVIIPEPLRPFMGGLELISPTSA 626
           KGNLAPTQFVHTLNATACAVPRMIVCLLENYQQEDGSVIIPEPLRPFMGGL+LISPTSA
Sbjct: 541 KGNLAPTQFVHTLNATACAVPRMIVCLLENYQQEDGSVIIPEPLRPFMGGLDLISPTSA 515

[Close]

BLAST of Lsi04G022480 vs. ExPASy TrEMBL
Match: A0A6J1FJG1 (Seryl-tRNA synthetase OS=Cucurbita moschata OX=3662 GN=LOC111445913 PE=4 SV=1)

HSP 1 Score: 927.5 bits (2396), Expect = 2.8e-266
Identity = 492/634 (77.60%), Postives = 513/634 (80.91%), Query Frame = 0


Query: 1   MKIIGARAAIRYFHSFPPSKNFLKAPTMGF---------QTLKFAAFPASSTSRLIFRPP 60
           MKII ARAAIRYFH+FPPSK+F +APTMGF         QTLK AAFP  +TSRLIFR P
Sbjct: 1   MKIIEARAAIRYFHTFPPSKSFRRAPTMGFHSSLSATSLQTLKLAAFP--TTSRLIFRLP 60

Query: 61  TKTPSLSSLHPSFRSHRPPSQLLVRALSLSAVQVDPATAIAGEEKVVKPQWKASIDFKWI 120
           TKT SL+SLHP FR+ +PP  LLVRALS SAVQVDP TA AGEE V KPQWKASIDFKWI
Sbjct: 61  TKTASLNSLHPCFRNRKPPCPLLVRALSFSAVQVDPPTATAGEEIVGKPQWKASIDFKWI 120

Query: 121 RDNKDLVASNIANRKSGANLELVLQLYDKMLNLQKEVERLRAERNVVANKMKGKLEPSER 180
           RDNKDLVA+NI NRKSGANLELVLQLYDKMLNLQKEVERLRAERN+VANKMKGKLEPSER
Sbjct: 121 RDNKDLVAANIENRKSGANLELVLQLYDKMLNLQKEVERLRAERNMVANKMKGKLEPSER 180

Query: 181 QKLIEEGKNLKEGLVALEENLLKLTEQLQQEAQCIPNTTHPDVPVGGEDCSIIRKMVGSP 240
           QKLIEEGKNLKEGLV+LEENLLKLT+QLQQEAQCIPN THPDVP+GGEDCSIIRK VGSP
Sbjct: 181 QKLIEEGKNLKEGLVSLEENLLKLTDQLQQEAQCIPNITHPDVPIGGEDCSIIRKTVGSP 240

Query: 241 REFSFPVKDHLELGKQLNLFDFDAAAEVQSLFSLRYLRAASSSCYVHLKILHRALLHPPF 300
           REF F VKDHLELGKQLNLFDFDAAAE                                 
Sbjct: 241 REFGFSVKDHLELGKQLNLFDFDAAAE--------------------------------- 300

Query: 301 LFVNAYHSICRPRIRYYAYEYWFSCSLSLYTTLPLLFISLTYVFECAHGLNVSGSKFYYL 360
                                                              VSGSKFYYL
Sbjct: 301 ---------------------------------------------------VSGSKFYYL 360

Query: 361 KNAAVTLEMGLINWTLSEVMKRGFTPLITPEIVRSTVVEKCGFQPRGENTQVYSIEGSDQ 420
           KNAAVTLEMGLI+WTLSEVMKRGFTP+ITPEIVRS+VVEKCGFQPRGENTQVYSIEGSDQ
Sbjct: 361 KNAAVTLEMGLISWTLSEVMKRGFTPVITPEIVRSSVVEKCGFQPRGENTQVYSIEGSDQ 420

Query: 421 CLIGTAEIPVGGIHMDSILSESSLPLKYVAYSHCFRTEAGAAGAATRGLYRVHQFSKVEM 480
           CLIGTAEIPVGGIHMDSIL+ESSLPLKYVAYSHCFRTEAGAAGAATRGLYRVHQFSKVEM
Sbjct: 421 CLIGTAEIPVGGIHMDSILAESSLPLKYVAYSHCFRTEAGAAGAATRGLYRVHQFSKVEM 480

Query: 481 FIFCRPEESESYHEELIRIEEDLFSSLGLHFKTLDMASADLGAPAYRKFDVEAWMPGLGR 540
           FI CRPE+SESY EELI IEEDLF+SLGLHFKTLDMAS DLGAPAYRKFDVEAWMPGLGR
Sbjct: 481 FILCRPEDSESYLEELISIEEDLFTSLGLHFKTLDMASGDLGAPAYRKFDVEAWMPGLGR 540

Query: 541 YGEISSASNCTDYQSRRLGIRYRPQEPVPTTS-KKSKGNLAPTQFVHTLNATACAVPRMI 600
           YGEISSASNCTDYQSRRLGIRYRPQE +PTTS KKSKGNLAPTQFVHTLNATACAVPRMI
Sbjct: 541 YGEISSASNCTDYQSRRLGIRYRPQESIPTTSKKKSKGNLAPTQFVHTLNATACAVPRMI 548

Query: 601 VCLLENYQQEDGSVIIPEPLRPFMGGLELISPTS 625
           VCLLENYQQEDGSVIIPE LRPFMGGLE+I+PTS
Sbjct: 601 VCLLENYQQEDGSVIIPESLRPFMGGLEIIAPTS 548

[Close]

BLAST of Lsi04G022480 vs. ExPASy TrEMBL
Match: A0A6J1BV32 (Seryl-tRNA synthetase OS=Momordica charantia OX=3673 GN=LOC111006001 PE=4 SV=1)

HSP 1 Score: 885.2 bits (2286), Expect = 1.6e-253
Identity = 463/598 (77.42%), Postives = 483/598 (80.77%), Query Frame = 0


Query: 30  FQTLKFAAFPASSTSRLIFRPPTKTPSLSSLHPSF--RSHRPPSQLLVRALSLSAVQVDP 89
           FQTLK AAFP  STSRLIFRP    PSL+SL+P F     RPP  LL+RALSL A+Q DP
Sbjct: 6   FQTLKLAAFPTPSTSRLIFRP----PSLNSLNPHFCRNKRRPPIPLLLRALSLPALQADP 65

Query: 90  ATAIAGEEKVVKPQWKASIDFKWIRDNKDLVASNIANRKSGANLELVLQLYDKMLNLQKE 149
            T IAGEEKV KPQW+ASIDFKWIRDN+DLVA+NI NRKSG +LELVLQLYDKMLNLQKE
Sbjct: 66  PTTIAGEEKVAKPQWRASIDFKWIRDNRDLVAANILNRKSGVDLELVLQLYDKMLNLQKE 125

Query: 150 VERLRAERNVVANKMKGKLEPSERQKLIEEGKNLKEGLVALEENLLKLTEQLQQEAQCIP 209
           VERLRAERN VANKMKGKLEPSERQKLIEEGKNLKEGLV+LEENLLKLT+QLQQEAQCIP
Sbjct: 126 VERLRAERNAVANKMKGKLEPSERQKLIEEGKNLKEGLVSLEENLLKLTDQLQQEAQCIP 185

Query: 210 NTTHPDVPVGGEDCSIIRKMVGSPREFSFPVKDHLELGKQLNLFDFDAAAEVQSLFSLRY 269
           N THPDVP+GGE+CS IRK VG+PREFSFPVKDHLELGK+LNLFDFDAAAE         
Sbjct: 186 NMTHPDVPIGGEECSTIRKTVGTPREFSFPVKDHLELGKRLNLFDFDAAAE--------- 245

Query: 270 LRAASSSCYVHLKILHRALLHPPFLFVNAYHSICRPRIRYYAYEYWFSCSLSLYTTLPLL 329
                                                                       
Sbjct: 246 ------------------------------------------------------------ 305

Query: 330 FISLTYVFECAHGLNVSGSKFYYLKNAAVTLEMGLINWTLSEVMKRGFTPLITPEIVRST 389
                          VSGSKFYYLKNAAVTLE+GLINWTLSEVMKRGFTPLITPEIVRS+
Sbjct: 306 ---------------VSGSKFYYLKNAAVTLELGLINWTLSEVMKRGFTPLITPEIVRSS 365

Query: 390 VVEKCGFQPRGENTQVYSIEGSDQCLIGTAEIPVGGIHMDSILSESSLPLKYVAYSHCFR 449
           VVEKCGFQPRGENTQVYSIEGSDQCLIGTAEIPVGGIHMDSILSESSLPLKYVAYSHCFR
Sbjct: 366 VVEKCGFQPRGENTQVYSIEGSDQCLIGTAEIPVGGIHMDSILSESSLPLKYVAYSHCFR 425

Query: 450 TEAGAAGAATRGLYRVHQFSKVEMFIFCRPEESESYHEELIRIEEDLFSSLGLHFKTLDM 509
           TEAGAAGAATRGLYRVHQFSK+EMFIFCRPEESESYHEELIRIEE LFSSLGLHFKTLDM
Sbjct: 426 TEAGAAGAATRGLYRVHQFSKLEMFIFCRPEESESYHEELIRIEEHLFSSLGLHFKTLDM 485

Query: 510 ASADLGAPAYRKFDVEAWMPGLGRYGEISSASNCTDYQSRRLGIRYRPQEPVPTTSKKSK 569
           AS DLGAPAYRKFDVEAWMPGLGRYGEISSASNCTDYQSRRLGIRYRP EPVPTTSKK K
Sbjct: 486 ASEDLGAPAYRKFDVEAWMPGLGRYGEISSASNCTDYQSRRLGIRYRPHEPVPTTSKKGK 515

Query: 570 GNLAPTQFVHTLNATACAVPRMIVCLLENYQQEDGSVIIPEPLRPFMGGLELISPTSA 626
            NLAPTQFVHTLNATACA+PRMI+CLLENYQQEDGSVIIPEPLRPFMGGLELI+PTSA
Sbjct: 546 SNLAPTQFVHTLNATACAIPRMIICLLENYQQEDGSVIIPEPLRPFMGGLELIAPTSA 515

[Close]

BLAST of Lsi04G022480 vs. NCBI nr
Match: XP_004136430.2 (serine--tRNA ligase, chloroplastic/mitochondrial isoform X1 [Cucumis sativus])

HSP 1 Score: 952.6 bits (2461), Expect = 1.7e-273
Identity = 496/624 (79.49%), Postives = 516/624 (82.69%), Query Frame = 0


Query: 3   IIGARAAIRYFHSFPPSKNFLKAPTMGFQTLKFAAFPASSTSRLIFRPPTKTPSLSSLHP 62
           I+G R AIR+FHSFPPS  F KA TMGFQTLK  AFPASSTSRL+  PPTKTPSL+SLHP
Sbjct: 5   IMGERGAIRHFHSFPPSHTFPKASTMGFQTLKLPAFPASSTSRLMVLPPTKTPSLTSLHP 64

Query: 63  SFRSHRPPSQLLVRALSLSAVQVD-PATAIAGEEKVVKPQWKASIDFKWIRDNKDLVASN 122
             R+HRPPS+LLVRA SL +V+ D P + IAGEEKVVKPQWKASIDFKWIRDNKDLVASN
Sbjct: 65  CVRTHRPPSRLLVRAFSLPSVEFDHPPSTIAGEEKVVKPQWKASIDFKWIRDNKDLVASN 124

Query: 123 IANRKSGANLELVLQLYDKMLNLQKEVERLRAERNVVANKMKGKLEPSERQKLIEEGKNL 182
           I NRKSGANLELVLQLYDKMLNLQKEVE+LRAERN+VANKMKGKLEPSERQKLIEEGKNL
Sbjct: 125 IQNRKSGANLELVLQLYDKMLNLQKEVEQLRAERNMVANKMKGKLEPSERQKLIEEGKNL 184

Query: 183 KEGLVALEENLLKLTEQLQQEAQCIPNTTHPDVPVGGEDCSIIRKMVGSPREFSFPVKDH 242
           KEGLV+LEENLLKLT+QLQQEAQCIPNTTHPDVP+GGEDCSIIRKMVGSPREFSFPVKDH
Sbjct: 185 KEGLVSLEENLLKLTDQLQQEAQCIPNTTHPDVPIGGEDCSIIRKMVGSPREFSFPVKDH 244

Query: 243 LELGKQLNLFDFDAAAEVQSLFSLRYLRAASSSCYVHLKILHRALLHPPFLFVNAYHSIC 302
           LELGKQL++FDFDAAAE                                           
Sbjct: 245 LELGKQLDIFDFDAAAE------------------------------------------- 304

Query: 303 RPRIRYYAYEYWFSCSLSLYTTLPLLFISLTYVFECAHGLNVSGSKFYYLKNAAVTLEMG 362
                                                    VSGSKFYYLKNAAVTLE+G
Sbjct: 305 -----------------------------------------VSGSKFYYLKNAAVTLELG 364

Query: 363 LINWTLSEVMKRGFTPLITPEIVRSTVVEKCGFQPRGENTQVYSIEGSDQCLIGTAEIPV 422
           LINWTLSEVMKRGFTPLITPEIVRSTVVEKCGFQPRGENTQVYSIEGSDQCLIGTAEIPV
Sbjct: 365 LINWTLSEVMKRGFTPLITPEIVRSTVVEKCGFQPRGENTQVYSIEGSDQCLIGTAEIPV 424

Query: 423 GGIHMDSILSESSLPLKYVAYSHCFRTEAGAAGAATRGLYRVHQFSKVEMFIFCRPEESE 482
           GGIHMDSILSESSLPLKYVAYSHCFRTEAGAAGAATRGLYRVHQFSKVEMFIFCRPEESE
Sbjct: 425 GGIHMDSILSESSLPLKYVAYSHCFRTEAGAAGAATRGLYRVHQFSKVEMFIFCRPEESE 484

Query: 483 SYHEELIRIEEDLFSSLGLHFKTLDMASADLGAPAYRKFDVEAWMPGLGRYGEISSASNC 542
           SYHEELIRIEEDLFSSLGLHFKTLDMASADLGAPAYRKFDVEAWMPGLGRYGEISSASNC
Sbjct: 485 SYHEELIRIEEDLFSSLGLHFKTLDMASADLGAPAYRKFDVEAWMPGLGRYGEISSASNC 544

Query: 543 TDYQSRRLGIRYRPQEPVPTTSKKSKGNLAPTQFVHTLNATACAVPRMIVCLLENYQQED 602
           TDYQSRRLGIRYRPQE VP+T KK KGNLAPTQFVHTLNATACAVPRM+VCLLENYQQED
Sbjct: 545 TDYQSRRLGIRYRPQEQVPSTLKKGKGNLAPTQFVHTLNATACAVPRMMVCLLENYQQED 544

Query: 603 GSVIIPEPLRPFMGGLELISPTSA 626
           GSVIIPEPLRPFMGGL+ ISP SA
Sbjct: 605 GSVIIPEPLRPFMGGLDRISPASA 544

[Close]

BLAST of Lsi04G022480 vs. NCBI nr
Match: KAG6608257.1 (Serine--tRNA ligase, chloroplastic/mitochondrial, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 934.9 bits (2415), Expect = 3.7e-268
Identity = 494/634 (77.92%), Postives = 515/634 (81.23%), Query Frame = 0


Query: 1   MKIIGARAAIRYFHSFPPSKNFLKAPTMGF---------QTLKFAAFPASSTSRLIFRPP 60
           MKII ARAAIRYFH+FPPSK+F +APTMGF         QTLK AAFP  +TSRLIFR P
Sbjct: 1   MKIIEARAAIRYFHTFPPSKSFRRAPTMGFHSSFSGTSLQTLKLAAFP--TTSRLIFRLP 60

Query: 61  TKTPSLSSLHPSFRSHRPPSQLLVRALSLSAVQVDPATAIAGEEKVVKPQWKASIDFKWI 120
           TKT SL+SLHP FR+ +PP  LLVRALS SAVQVDP TA AGEE V KPQWKASIDFKWI
Sbjct: 61  TKTASLNSLHPCFRNRKPPCPLLVRALSFSAVQVDPPTATAGEEIVAKPQWKASIDFKWI 120

Query: 121 RDNKDLVASNIANRKSGANLELVLQLYDKMLNLQKEVERLRAERNVVANKMKGKLEPSER 180
           RDNKDLVA+NI NRKSGANLELVLQLYDKMLNLQKEVERLRAERN+VANKMKGKLEPSER
Sbjct: 121 RDNKDLVAANIENRKSGANLELVLQLYDKMLNLQKEVERLRAERNMVANKMKGKLEPSER 180

Query: 181 QKLIEEGKNLKEGLVALEENLLKLTEQLQQEAQCIPNTTHPDVPVGGEDCSIIRKMVGSP 240
           QKLIEEGKNLKEGLV+LEENLLKLT+QLQQEAQCIPN THPDVP+GGEDCSIIRK VGSP
Sbjct: 181 QKLIEEGKNLKEGLVSLEENLLKLTDQLQQEAQCIPNITHPDVPIGGEDCSIIRKTVGSP 240

Query: 241 REFSFPVKDHLELGKQLNLFDFDAAAEVQSLFSLRYLRAASSSCYVHLKILHRALLHPPF 300
           REF F VKDHLELGKQLNLFDFDAAAE                                 
Sbjct: 241 REFGFSVKDHLELGKQLNLFDFDAAAE--------------------------------- 300

Query: 301 LFVNAYHSICRPRIRYYAYEYWFSCSLSLYTTLPLLFISLTYVFECAHGLNVSGSKFYYL 360
                                                              VSGSKFYYL
Sbjct: 301 ---------------------------------------------------VSGSKFYYL 360

Query: 361 KNAAVTLEMGLINWTLSEVMKRGFTPLITPEIVRSTVVEKCGFQPRGENTQVYSIEGSDQ 420
           KNAAVTLEMGLI+WTLSEVMKRGFTP+ITPEIVRS+VVEKCGFQPRGENTQVYSIEGSDQ
Sbjct: 361 KNAAVTLEMGLISWTLSEVMKRGFTPVITPEIVRSSVVEKCGFQPRGENTQVYSIEGSDQ 420

Query: 421 CLIGTAEIPVGGIHMDSILSESSLPLKYVAYSHCFRTEAGAAGAATRGLYRVHQFSKVEM 480
           CLIGTAEIPVGGIHMDSIL+ESSLPLKYVAYSHCFRTEAGAAGAATRGLYRVHQFSKVEM
Sbjct: 421 CLIGTAEIPVGGIHMDSILAESSLPLKYVAYSHCFRTEAGAAGAATRGLYRVHQFSKVEM 480

Query: 481 FIFCRPEESESYHEELIRIEEDLFSSLGLHFKTLDMASADLGAPAYRKFDVEAWMPGLGR 540
           FI CRPE+SESY EELI IEEDLF+SLGLHFKTLDMAS DLGAPAYRKFDVEAWMPGLGR
Sbjct: 481 FILCRPEDSESYLEELISIEEDLFTSLGLHFKTLDMASGDLGAPAYRKFDVEAWMPGLGR 540

Query: 541 YGEISSASNCTDYQSRRLGIRYRPQEPVPTTS-KKSKGNLAPTQFVHTLNATACAVPRMI 600
           YGEISSASNCTDYQSRRLGIRYRPQEP+PTTS KKSKGNLAPTQFVHTLNATACAVPRMI
Sbjct: 541 YGEISSASNCTDYQSRRLGIRYRPQEPIPTTSKKKSKGNLAPTQFVHTLNATACAVPRMI 548

Query: 601 VCLLENYQQEDGSVIIPEPLRPFMGGLELISPTS 625
           VCLLENYQQEDGSVIIPEPLRPFMGGLE+I+PTS
Sbjct: 601 VCLLENYQQEDGSVIIPEPLRPFMGGLEIIAPTS 548

[Close]

BLAST of Lsi04G022480 vs. NCBI nr
Match: XP_008466212.1 (PREDICTED: serine--tRNA ligase, chloroplastic/mitochondrial [Cucumis melo])

HSP 1 Score: 932.2 bits (2408), Expect = 2.4e-267
Identity = 486/603 (80.60%), Postives = 502/603 (83.25%), Query Frame = 0


Query: 24  KAPTMGFQTLKFAAFPASSTSRLIFRPPTKTPSLSSLHPSFRSHRPPSQLLVRALSLSAV 83
           KA TMGFQTLK A FPASSTSRL+F PPTKTPSL+  HP  R+HRPPS+L VRA SL +V
Sbjct: 43  KASTMGFQTLKLADFPASSTSRLMFLPPTKTPSLTPFHPCVRTHRPPSRLFVRAFSLPSV 102

Query: 84  QVD-PATAIAGEEKVVKPQWKASIDFKWIRDNKDLVASNIANRKSGANLELVLQLYDKML 143
           QVD P T IAGEEKVVKPQWKASIDFKWIRDNKDLVASNI NRKSGANLELVLQLYD+ML
Sbjct: 103 QVDHPPTTIAGEEKVVKPQWKASIDFKWIRDNKDLVASNIHNRKSGANLELVLQLYDEML 162

Query: 144 NLQKEVERLRAERNVVANKMKGKLEPSERQKLIEEGKNLKEGLVALEENLLKLTEQLQQE 203
           NLQKEVE+LRAERNVVANKMKGKLEPSERQKLIEEGKNLKEGLV+LEENLLKLT+QLQQE
Sbjct: 163 NLQKEVEQLRAERNVVANKMKGKLEPSERQKLIEEGKNLKEGLVSLEENLLKLTDQLQQE 222

Query: 204 AQCIPNTTHPDVPVGGEDCSIIRKMVGSPREFSFPVKDHLELGKQLNLFDFDAAAEVQSL 263
           AQCIPNTTHPDVP+GGEDCSIIRKMVGSPREFSFPVKDHLELG+QL++FDFDAAAE    
Sbjct: 223 AQCIPNTTHPDVPIGGEDCSIIRKMVGSPREFSFPVKDHLELGRQLDIFDFDAAAE---- 282

Query: 264 FSLRYLRAASSSCYVHLKILHRALLHPPFLFVNAYHSICRPRIRYYAYEYWFSCSLSLYT 323
                                                                       
Sbjct: 283 ------------------------------------------------------------ 342

Query: 324 TLPLLFISLTYVFECAHGLNVSGSKFYYLKNAAVTLEMGLINWTLSEVMKRGFTPLITPE 383
                               VSGSKFYYLKNAAVTLE+GLINWTLSEVMKRGFTPLITPE
Sbjct: 343 --------------------VSGSKFYYLKNAAVTLELGLINWTLSEVMKRGFTPLITPE 402

Query: 384 IVRSTVVEKCGFQPRGENTQVYSIEGSDQCLIGTAEIPVGGIHMDSILSESSLPLKYVAY 443
           IVRSTVVEKCGFQPRGENTQVYSIEGSDQCLIGTAEIPVGGIHMDSILSESSLPLKYVAY
Sbjct: 403 IVRSTVVEKCGFQPRGENTQVYSIEGSDQCLIGTAEIPVGGIHMDSILSESSLPLKYVAY 462

Query: 444 SHCFRTEAGAAGAATRGLYRVHQFSKVEMFIFCRPEESESYHEELIRIEEDLFSSLGLHF 503
           SHCFRTEAGAAGAATRGLYRVHQFSKVEMFIFCRPEESESYHEELIRIEEDLFSSLGLHF
Sbjct: 463 SHCFRTEAGAAGAATRGLYRVHQFSKVEMFIFCRPEESESYHEELIRIEEDLFSSLGLHF 522

Query: 504 KTLDMASADLGAPAYRKFDVEAWMPGLGRYGEISSASNCTDYQSRRLGIRYRPQEPVPTT 563
           KTLDMASADLGAPAYRKFDVEAWMPGLGRYGEISSASNCTDYQSRRLGIRYRPQEPVP+T
Sbjct: 523 KTLDMASADLGAPAYRKFDVEAWMPGLGRYGEISSASNCTDYQSRRLGIRYRPQEPVPST 561

Query: 564 SKKSKGNLAPTQFVHTLNATACAVPRMIVCLLENYQQEDGSVIIPEPLRPFMGGLELISP 623
            KK KGNLAPTQFVHTLNATACAVPRMIVCLLENYQQEDGSVIIPEPLRPFMGGL+LISP
Sbjct: 583 LKKGKGNLAPTQFVHTLNATACAVPRMIVCLLENYQQEDGSVIIPEPLRPFMGGLDLISP 561

Query: 624 TSA 626
           TSA
Sbjct: 643 TSA 561

[Close]

BLAST of Lsi04G022480 vs. NCBI nr
Match: XP_023524141.1 (serine--tRNA ligase, chloroplastic/mitochondrial isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 928.3 bits (2398), Expect = 3.4e-266
Identity = 491/634 (77.44%), Postives = 514/634 (81.07%), Query Frame = 0


Query: 1   MKIIGARAAIRYFHSFPPSKNFLKAPTMGF---------QTLKFAAFPASSTSRLIFRPP 60
           MKII ARAAIRYFH+FPPSK+FL+APTMGF         +TLK AAFP  +TSRLIFR P
Sbjct: 1   MKIIEARAAIRYFHTFPPSKSFLRAPTMGFHSSFSVTSLKTLKVAAFP--TTSRLIFRLP 60

Query: 61  TKTPSLSSLHPSFRSHRPPSQLLVRALSLSAVQVDPATAIAGEEKVVKPQWKASIDFKWI 120
           TKT SL+SLHP FR+ +PP  LLVRALS S VQVDP TA AGEE V KPQWKASIDFKWI
Sbjct: 61  TKTASLNSLHPCFRNRKPPCPLLVRALSFSEVQVDPPTATAGEEIVAKPQWKASIDFKWI 120

Query: 121 RDNKDLVASNIANRKSGANLELVLQLYDKMLNLQKEVERLRAERNVVANKMKGKLEPSER 180
           RDNKDLVA+NI NRKSGANLELVLQLYDKMLNLQKEVERLRAERN+VANKMKGKLEPSER
Sbjct: 121 RDNKDLVAANIENRKSGANLELVLQLYDKMLNLQKEVERLRAERNMVANKMKGKLEPSER 180

Query: 181 QKLIEEGKNLKEGLVALEENLLKLTEQLQQEAQCIPNTTHPDVPVGGEDCSIIRKMVGSP 240
           QKLIEEGKNLKEGLV+LEENLLKLT+QLQQEAQCIPN THPDVP+GGEDCSIIRK VGSP
Sbjct: 181 QKLIEEGKNLKEGLVSLEENLLKLTDQLQQEAQCIPNITHPDVPIGGEDCSIIRKTVGSP 240

Query: 241 REFSFPVKDHLELGKQLNLFDFDAAAEVQSLFSLRYLRAASSSCYVHLKILHRALLHPPF 300
           REF F VKDHLELGKQLNLFDFDAAAE                                 
Sbjct: 241 REFGFSVKDHLELGKQLNLFDFDAAAE--------------------------------- 300

Query: 301 LFVNAYHSICRPRIRYYAYEYWFSCSLSLYTTLPLLFISLTYVFECAHGLNVSGSKFYYL 360
                                                              VSGSKFYYL
Sbjct: 301 ---------------------------------------------------VSGSKFYYL 360

Query: 361 KNAAVTLEMGLINWTLSEVMKRGFTPLITPEIVRSTVVEKCGFQPRGENTQVYSIEGSDQ 420
           KNAAVTLEMGLI+WTLSEVMKRGFTP+ITPEIVRS+VVEKCGFQPRGENTQVYSIEGSDQ
Sbjct: 361 KNAAVTLEMGLISWTLSEVMKRGFTPVITPEIVRSSVVEKCGFQPRGENTQVYSIEGSDQ 420

Query: 421 CLIGTAEIPVGGIHMDSILSESSLPLKYVAYSHCFRTEAGAAGAATRGLYRVHQFSKVEM 480
           CLIGTAEIPVGGIHMDSIL+ESSLPLKYVAYSHCFRTEAGAAGAATRGLYRVHQFSKVEM
Sbjct: 421 CLIGTAEIPVGGIHMDSILAESSLPLKYVAYSHCFRTEAGAAGAATRGLYRVHQFSKVEM 480

Query: 481 FIFCRPEESESYHEELIRIEEDLFSSLGLHFKTLDMASADLGAPAYRKFDVEAWMPGLGR 540
           FI CRPE+SESY EELI IEEDLF+SLGLHFKTLDMAS DLGAPAYRKFDVEAWMPGLGR
Sbjct: 481 FILCRPEDSESYLEELISIEEDLFTSLGLHFKTLDMASGDLGAPAYRKFDVEAWMPGLGR 540

Query: 541 YGEISSASNCTDYQSRRLGIRYRPQEPVPTTS-KKSKGNLAPTQFVHTLNATACAVPRMI 600
           YGEISSASNCTDYQSRRLGIRYRPQE +PTTS KK+KGNLAPTQFVHTLNATACAVPRMI
Sbjct: 541 YGEISSASNCTDYQSRRLGIRYRPQELIPTTSKKKNKGNLAPTQFVHTLNATACAVPRMI 548

Query: 601 VCLLENYQQEDGSVIIPEPLRPFMGGLELISPTS 625
           VCLLENYQQEDGSVIIPEPLRPFMGGLE+I+PTS
Sbjct: 601 VCLLENYQQEDGSVIIPEPLRPFMGGLEIIAPTS 548

[Close]

BLAST of Lsi04G022480 vs. NCBI nr
Match: XP_022940229.1 (serine--tRNA ligase, chloroplastic/mitochondrial isoform X1 [Cucurbita moschata])

HSP 1 Score: 927.5 bits (2396), Expect = 5.9e-266
Identity = 492/634 (77.60%), Postives = 513/634 (80.91%), Query Frame = 0


Query: 1   MKIIGARAAIRYFHSFPPSKNFLKAPTMGF---------QTLKFAAFPASSTSRLIFRPP 60
           MKII ARAAIRYFH+FPPSK+F +APTMGF         QTLK AAFP  +TSRLIFR P
Sbjct: 1   MKIIEARAAIRYFHTFPPSKSFRRAPTMGFHSSLSATSLQTLKLAAFP--TTSRLIFRLP 60

Query: 61  TKTPSLSSLHPSFRSHRPPSQLLVRALSLSAVQVDPATAIAGEEKVVKPQWKASIDFKWI 120
           TKT SL+SLHP FR+ +PP  LLVRALS SAVQVDP TA AGEE V KPQWKASIDFKWI
Sbjct: 61  TKTASLNSLHPCFRNRKPPCPLLVRALSFSAVQVDPPTATAGEEIVGKPQWKASIDFKWI 120

Query: 121 RDNKDLVASNIANRKSGANLELVLQLYDKMLNLQKEVERLRAERNVVANKMKGKLEPSER 180
           RDNKDLVA+NI NRKSGANLELVLQLYDKMLNLQKEVERLRAERN+VANKMKGKLEPSER
Sbjct: 121 RDNKDLVAANIENRKSGANLELVLQLYDKMLNLQKEVERLRAERNMVANKMKGKLEPSER 180

Query: 181 QKLIEEGKNLKEGLVALEENLLKLTEQLQQEAQCIPNTTHPDVPVGGEDCSIIRKMVGSP 240
           QKLIEEGKNLKEGLV+LEENLLKLT+QLQQEAQCIPN THPDVP+GGEDCSIIRK VGSP
Sbjct: 181 QKLIEEGKNLKEGLVSLEENLLKLTDQLQQEAQCIPNITHPDVPIGGEDCSIIRKTVGSP 240

Query: 241 REFSFPVKDHLELGKQLNLFDFDAAAEVQSLFSLRYLRAASSSCYVHLKILHRALLHPPF 300
           REF F VKDHLELGKQLNLFDFDAAAE                                 
Sbjct: 241 REFGFSVKDHLELGKQLNLFDFDAAAE--------------------------------- 300

Query: 301 LFVNAYHSICRPRIRYYAYEYWFSCSLSLYTTLPLLFISLTYVFECAHGLNVSGSKFYYL 360
                                                              VSGSKFYYL
Sbjct: 301 ---------------------------------------------------VSGSKFYYL 360

Query: 361 KNAAVTLEMGLINWTLSEVMKRGFTPLITPEIVRSTVVEKCGFQPRGENTQVYSIEGSDQ 420
           KNAAVTLEMGLI+WTLSEVMKRGFTP+ITPEIVRS+VVEKCGFQPRGENTQVYSIEGSDQ
Sbjct: 361 KNAAVTLEMGLISWTLSEVMKRGFTPVITPEIVRSSVVEKCGFQPRGENTQVYSIEGSDQ 420

Query: 421 CLIGTAEIPVGGIHMDSILSESSLPLKYVAYSHCFRTEAGAAGAATRGLYRVHQFSKVEM 480
           CLIGTAEIPVGGIHMDSIL+ESSLPLKYVAYSHCFRTEAGAAGAATRGLYRVHQFSKVEM
Sbjct: 421 CLIGTAEIPVGGIHMDSILAESSLPLKYVAYSHCFRTEAGAAGAATRGLYRVHQFSKVEM 480

Query: 481 FIFCRPEESESYHEELIRIEEDLFSSLGLHFKTLDMASADLGAPAYRKFDVEAWMPGLGR 540
           FI CRPE+SESY EELI IEEDLF+SLGLHFKTLDMAS DLGAPAYRKFDVEAWMPGLGR
Sbjct: 481 FILCRPEDSESYLEELISIEEDLFTSLGLHFKTLDMASGDLGAPAYRKFDVEAWMPGLGR 540

Query: 541 YGEISSASNCTDYQSRRLGIRYRPQEPVPTTS-KKSKGNLAPTQFVHTLNATACAVPRMI 600
           YGEISSASNCTDYQSRRLGIRYRPQE +PTTS KKSKGNLAPTQFVHTLNATACAVPRMI
Sbjct: 541 YGEISSASNCTDYQSRRLGIRYRPQESIPTTSKKKSKGNLAPTQFVHTLNATACAVPRMI 548

Query: 601 VCLLENYQQEDGSVIIPEPLRPFMGGLELISPTS 625
           VCLLENYQQEDGSVIIPE LRPFMGGLE+I+PTS
Sbjct: 601 VCLLENYQQEDGSVIIPESLRPFMGGLEIIAPTS 548

[Close]

BLAST of Lsi04G022480 vs. TAIR 10
Match: AT1G11870.2 (Seryl-tRNA synthetase )

HSP 1 Score: 724.2 bits (1868), Expect = 9.2e-209
Identity = 381/597 (63.82%), Postives = 433/597 (72.53%), Query Frame = 0


Query: 28  MGFQTLKF-AAFPASSTSRLIFRPPTKTPSLSSLHPSFRSHRPPSQLLVRALSLS-AVQV 87
           MG   L+  AA P + +SRL  +P T T +L       +    P  LLVRA S S AVQ 
Sbjct: 1   MGLHKLRLAAAVPVTFSSRLFLKPFTNTLTLGLFSRHLQPRNKP--LLVRAFSASAAVQD 60

Query: 88  DPATAIAGEEKVVKPQWKASIDFKWIRDNKDLVASNIANRKSGANLELVLQLYDKMLNLQ 147
            PAT  +      +P WKA+IDFKWIRDNK+ V  NI NR S ANLE VLQLY+ M+NLQ
Sbjct: 61  IPATQTSDSSVAARPLWKAAIDFKWIRDNKEAVEINIRNRNSNANLEAVLQLYENMVNLQ 120

Query: 148 KEVERLRAERNVVANKMKGKLEPSERQKLIEEGKNLKEGLVALEENLLKLTEQLQQEAQC 207
           KEVERLR ERN VA KMKGKLEPSER++L+EEGKNLKE LV LEE+L+KL ++LQ  AQ 
Sbjct: 121 KEVERLREERNNVAKKMKGKLEPSERERLVEEGKNLKESLVTLEEDLVKLKDELQHVAQS 180

Query: 208 IPNTTHPDVPVGGEDCSIIRKMVGSPREFSFPVKDHLELGKQLNLFDFDAAAEVQSLFSL 267
           IPN THPDVPVGGED S IR+ VGSPREFSFP+KDHL+LGK L+L DFD+AAE       
Sbjct: 181 IPNMTHPDVPVGGEDSSAIRQEVGSPREFSFPIKDHLQLGKDLDLIDFDSAAE------- 240

Query: 268 RYLRAASSSCYVHLKILHRALLHPPFLFVNAYHSICRPRIRYYAYEYWFSCSLSLYTTLP 327
                                                                       
Sbjct: 241 ------------------------------------------------------------ 300

Query: 328 LLFISLTYVFECAHGLNVSGSKFYYLKNAAVTLEMGLINWTLSEVMKRGFTPLITPEIVR 387
                            VSGSKF+YLKN AV LEM L+NWTLS+VMK+G+TPL TPEIVR
Sbjct: 301 -----------------VSGSKFFYLKNEAVLLEMALLNWTLSQVMKKGYTPLTTPEIVR 360

Query: 388 STVVEKCGFQPRGENTQVYSIEGSDQCLIGTAEIPVGGIHMDSILSESSLPLKYVAYSHC 447
           S++VEKCGFQPRG+NTQVYSI+G+DQCLIGTAEIPVGGIHMDSIL ES+LPLKY+A+SHC
Sbjct: 361 SSIVEKCGFQPRGDNTQVYSIDGTDQCLIGTAEIPVGGIHMDSILLESALPLKYIAFSHC 420

Query: 448 FRTEAGAAGAATRGLYRVHQFSKVEMFIFCRPEESESYHEELIRIEEDLFSSLGLHFKTL 507
           FRTEAGAAGAAT+GLYRVHQFSK EMF+ C+PE+SES+H+ELI+IEEDLF+SLGLHFKTL
Sbjct: 421 FRTEAGAAGAATKGLYRVHQFSKAEMFVICQPEDSESFHQELIQIEEDLFTSLGLHFKTL 480

Query: 508 DMASADLGAPAYRKFDVEAWMPGLGRYGEISSASNCTDYQSRRLGIRYRPQEPVPTTSKK 567
           DMA+ADLGAPAYRKFD+EAWMPGLGR+GEISSASNCTDYQSRRLGIRYRP EP  T  KK
Sbjct: 481 DMATADLGAPAYRKFDIEAWMPGLGRFGEISSASNCTDYQSRRLGIRYRPSEPPQTGPKK 511

Query: 568 SKGNLAPTQFVHTLNATACAVPRMIVCLLENYQQEDGSVIIPEPLRPFMGGLELISP 623
            K NL  T+FVHTLNATACAVPRM+VCLLENYQQEDGSV+IPEPLRPFMGG+ELI P
Sbjct: 541 GKANLPATKFVHTLNATACAVPRMMVCLLENYQQEDGSVVIPEPLRPFMGGIELIKP 511

[Close]

BLAST of Lsi04G022480 vs. TAIR 10
Match: AT1G11870.1 (Seryl-tRNA synthetase )

HSP 1 Score: 719.9 bits (1857), Expect = 1.7e-207
Identity = 381/597 (63.82%), Postives = 434/597 (72.70%), Query Frame = 0


Query: 28  MGFQTLKF-AAFPASSTSRLIFRPPTKTPSLSSLHPSFRSHRPPSQLLVRALSLS-AVQV 87
           MG   L+  AA P + +SRL  +P T T +L       +    P  LLVRA S S AVQ 
Sbjct: 1   MGLHKLRLAAAVPVTFSSRLFLKPFTNTLTLGLFSRHLQPRNKP--LLVRAFSASAAVQD 60

Query: 88  DPATAIAGEEKVVKPQWKASIDFKWIRDNKDLVASNIANRKSGANLELVLQLYDKMLNLQ 147
            PAT  +  +   +P WKA+IDFKWIRDNK+ V  NI NR S ANLE VLQLY+ M+NLQ
Sbjct: 61  IPATQTS--DSSARPLWKAAIDFKWIRDNKEAVEINIRNRNSNANLEAVLQLYENMVNLQ 120

Query: 148 KEVERLRAERNVVANKMKGKLEPSERQKLIEEGKNLKEGLVALEENLLKLTEQLQQEAQC 207
           KEVERLR ERN VA KMKGKLEPSER++L+EEGKNLKE LV LEE+L+KL ++LQ  AQ 
Sbjct: 121 KEVERLREERNNVAKKMKGKLEPSERERLVEEGKNLKESLVTLEEDLVKLKDELQHVAQS 180

Query: 208 IPNTTHPDVPVGGEDCSIIRKMVGSPREFSFPVKDHLELGKQLNLFDFDAAAEVQSLFSL 267
           IPN THPDVPVGGED S IR+ VGSPREFSFP+KDHL+LGK L+L DFD+AAE       
Sbjct: 181 IPNMTHPDVPVGGEDSSAIRQEVGSPREFSFPIKDHLQLGKDLDLIDFDSAAE------- 240

Query: 268 RYLRAASSSCYVHLKILHRALLHPPFLFVNAYHSICRPRIRYYAYEYWFSCSLSLYTTLP 327
                                                                       
Sbjct: 241 ------------------------------------------------------------ 300

Query: 328 LLFISLTYVFECAHGLNVSGSKFYYLKNAAVTLEMGLINWTLSEVMKRGFTPLITPEIVR 387
                            VSGSKF+YLKN AV LEM L+NWTLS+VMK+G+TPL TPEIVR
Sbjct: 301 -----------------VSGSKFFYLKNEAVLLEMALLNWTLSQVMKKGYTPLTTPEIVR 360

Query: 388 STVVEKCGFQPRGENTQVYSIEGSDQCLIGTAEIPVGGIHMDSILSESSLPLKYVAYSHC 447
           S++VEKCGFQPRG+NTQVYSI+G+DQCLIGTAEIPVGGIHMDSIL ES+LPLKY+A+SHC
Sbjct: 361 SSIVEKCGFQPRGDNTQVYSIDGTDQCLIGTAEIPVGGIHMDSILLESALPLKYIAFSHC 420

Query: 448 FRTEAGAAGAATRGLYRVHQFSKVEMFIFCRPEESESYHEELIRIEEDLFSSLGLHFKTL 507
           FRTEAGAAGAAT+GLYRVHQFSK EMF+ C+PE+SES+H+ELI+IEEDLF+SLGLHFKTL
Sbjct: 421 FRTEAGAAGAATKGLYRVHQFSKAEMFVICQPEDSESFHQELIQIEEDLFTSLGLHFKTL 480

Query: 508 DMASADLGAPAYRKFDVEAWMPGLGRYGEISSASNCTDYQSRRLGIRYRPQEPVPTTSKK 567
           DMA+ADLGAPAYRKFD+EAWMPGLGR+GEISSASNCTDYQSRRLGIRYRP EP  T  KK
Sbjct: 481 DMATADLGAPAYRKFDIEAWMPGLGRFGEISSASNCTDYQSRRLGIRYRPSEPPQTGPKK 509

Query: 568 SKGNLAPTQFVHTLNATACAVPRMIVCLLENYQQEDGSVIIPEPLRPFMGGLELISP 623
            K NL  T+FVHTLNATACAVPRM+VCLLENYQQEDGSV+IPEPLRPFMGG+ELI P
Sbjct: 541 GKANLPATKFVHTLNATACAVPRMMVCLLENYQQEDGSVVIPEPLRPFMGGIELIKP 509

[Close]

BLAST of Lsi04G022480 vs. TAIR 10
Match: AT1G11870.3 (Seryl-tRNA synthetase )

HSP 1 Score: 508.4 bits (1308), Expect = 7.9e-144
Identity = 279/484 (57.64%), Postives = 325/484 (67.15%), Query Frame = 0


Query: 28  MGFQTLKF-AAFPASSTSRLIFRPPTKTPSLSSLHPSFRSHRPPSQLLVRALSLS-AVQV 87
           MG   L+  AA P + +SRL  +P T T +L       +    P  LLVRA S S AVQ 
Sbjct: 1   MGLHKLRLAAAVPVTFSSRLFLKPFTNTLTLGLFSRHLQPRNKP--LLVRAFSASAAVQD 60

Query: 88  DPATAIAGEEKVVKPQWKASIDFKWIRDNKDLVASNIANRKSGANLELVLQLYDKMLNLQ 147
            PAT  +      +P WKA+IDFKWIRDNK+ V  NI NR S ANLE VLQLY+ M+NLQ
Sbjct: 61  IPATQTSDSSVAARPLWKAAIDFKWIRDNKEAVEINIRNRNSNANLEAVLQLYENMVNLQ 120

Query: 148 KEVERLRAERNVVANKMKGKLEPSERQKLIEEGKNLKEGLVALEENLLKLTEQLQQEAQC 207
           KEVERLR ERN VA KMKGKLEPSER++L+EEGKNLKE LV LEE+L+KL ++LQ  AQ 
Sbjct: 121 KEVERLREERNNVAKKMKGKLEPSERERLVEEGKNLKESLVTLEEDLVKLKDELQHVAQS 180

Query: 208 IPNTTHPDVPVGGEDCSIIRKMVGSPREFSFPVKDHLELGKQLNLFDFDAAAEVQSLFSL 267
           IPN THPDVPVGGED S IR+ VGSPREFSFP+KDHL+LGK L+L DFD+AAE       
Sbjct: 181 IPNMTHPDVPVGGEDSSAIRQEVGSPREFSFPIKDHLQLGKDLDLIDFDSAAE------- 240

Query: 268 RYLRAASSSCYVHLKILHRALLHPPFLFVNAYHSICRPRIRYYAYEYWFSCSLSLYTTLP 327
                                                                       
Sbjct: 241 ------------------------------------------------------------ 300

Query: 328 LLFISLTYVFECAHGLNVSGSKFYYLKNAAVTLEMGLINWTLSEVMKRGFTPLITPEIVR 387
                            VSGSKF+YLKN AV LEM L+NWTLS+VMK+G+TPL TPEIVR
Sbjct: 301 -----------------VSGSKFFYLKNEAVLLEMALLNWTLSQVMKKGYTPLTTPEIVR 360

Query: 388 STVVEKCGFQPRGENTQVYSIEGSDQCLIGTAEIPVGGIHMDSILSESSLPLKYVAYSHC 447
           S++VEKCGFQPRG+NTQVYSI+G+DQCLIGTAEIPVGGIHMDSIL ES+LPLKY+A+SHC
Sbjct: 361 SSIVEKCGFQPRGDNTQVYSIDGTDQCLIGTAEIPVGGIHMDSILLESALPLKYIAFSHC 398

Query: 448 FRTEAGAAGAATRGLYRVHQFSKVEMFIFCRPEESESYHEELIRIEEDLFSSLGLHFKTL 507
           FRTEAGAAGAAT+GLYRVHQFSK EMF+ C+PE+SES+H+ELI+IEEDLF+SLGLHF   
Sbjct: 421 FRTEAGAAGAATKGLYRVHQFSKAEMFVICQPEDSESFHQELIQIEEDLFTSLGLHFNRF 398

Query: 508 DMAS 510
             +S
Sbjct: 481 RRSS 398

[Close]

BLAST of Lsi04G022480 vs. TAIR 10
Match: AT5G27470.1 (seryl-tRNA synthetase / serine--tRNA ligase )

HSP 1 Score: 219.5 bits (558), Expect = 7.3e-57
Identity = 119/292 (40.75%), Postives = 172/292 (58.90%), Query Frame = 0


Query: 329 ISLTYVFECAHGLNVSGSKFYYLKNAAVTLEMGLINWTLSEVMKRGFTPLITPEIVRSTV 388
           + L  + +   G  ++G++ ++LK   + L   LIN+ L+ + KRGFT L  P  +R  V
Sbjct: 150 VELLGIADTKRGAEIAGARGFFLKGDGLMLNQALINFGLTFLKKRGFTGLQPPFFMRKDV 209

Query: 389 VEKCGFQPRGENTQVYSI--EGSDQCLIGTAEIPVGGIHMDSILSESSLPLKYVAYSHCF 448
           + KC  Q    + ++Y +  EG D+ LI TAE P+   H+D  +  + LPL+Y  YS CF
Sbjct: 210 MAKCA-QLAQFDEELYKVTGEGDDKYLIATAEQPLCAYHIDEWIHPTELPLRYAGYSSCF 269

Query: 449 RTEAGAAGAATRGLYRVHQFSKVEMFIFCRPEESESYH--EELIRIEEDLFSSLGLHFKT 508
           R EAG+ G  T G++RVHQF K+E F    P E+ S+   +E+++  ED + +L L ++ 
Sbjct: 270 RKEAGSHGRDTLGIFRVHQFEKIEQFCITGPNENASWEMLDEMMKNSEDFYQALKLPYQI 329

Query: 509 LDMASADLGAPAYRKFDVEAWMPGLGRYGEISSASNCTDYQSRRLGIRYRPQEPVPTTSK 568
           + + S  L   A +K+D+EAW P    + E+ S SNCTDYQ+RRL IRY           
Sbjct: 330 VSIVSGALNDAAAKKYDLEAWFPSSETFRELVSCSNCTDYQARRLEIRY----------G 389

Query: 569 KSKGNLAPTQFVHTLNATACAVPRMIVCLLENYQQEDGSVIIPEPLRPFMGG 617
           + K N    Q+VH LN+T  A  R I C+LENYQ+EDG V IPE L+PFMGG
Sbjct: 390 QKKSNEQTKQYVHMLNSTLTATERTICCILENYQREDG-VDIPEVLQPFMGG 429

The following BLAST results are available for this feature:

BLAST of Lsi04G022480 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8RWT8	1.3e-207	63.82	Serine--tRNA ligase, chloroplastic/mitochondrial OS=Arabidopsis thaliana OX=3702...	[more]
Q73KB2	3.7e-106	40.74	Serine--tRNA ligase OS=Treponema denticola (strain ATCC 35405 / DSM 14222 / CIP ...	[more]
Q1AX26	1.4e-97	39.38	Serine--tRNA ligase OS=Rubrobacter xylanophilus (strain DSM 9941 / NBRC 16129 / ...	[more]
B5RL50	7.1e-97	40.12	Serine--tRNA ligase OS=Borrelia duttonii (strain Ly) OX=412419 GN=serS PE=3 SV=1	[more]
B5RR43	4.6e-96	40.12	Serine--tRNA ligase OS=Borrelia recurrentis (strain A1) OX=412418 GN=serS PE=3 S...	[more]

BLAST of Lsi04G022480 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LHK3	8.2e-274	79.49	Seryl-tRNA synthetase OS=Cucumis sativus OX=3659 GN=Csa_3G889830 PE=4 SV=1	[more]
A0A1S3CQP4	1.2e-267	80.60	Seryl-tRNA synthetase OS=Cucumis melo OX=3656 GN=LOC103503692 PE=4 SV=1	[more]
A0A5A7TBE3	2.8e-266	80.63	Seryl-tRNA synthetase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold45...	[more]
A0A6J1FJG1	2.8e-266	77.60	Seryl-tRNA synthetase OS=Cucurbita moschata OX=3662 GN=LOC111445913 PE=4 SV=1	[more]
A0A6J1BV32	1.6e-253	77.42	Seryl-tRNA synthetase OS=Momordica charantia OX=3673 GN=LOC111006001 PE=4 SV=1	[more]

BLAST of Lsi04G022480 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004136430.2	1.7e-273	79.49	serine--tRNA ligase, chloroplastic/mitochondrial isoform X1 [Cucumis sativus]	[more]
KAG6608257.1	3.7e-268	77.92	Serine--tRNA ligase, chloroplastic/mitochondrial, partial [Cucurbita argyrosperm...	[more]
XP_008466212.1	2.4e-267	80.60	PREDICTED: serine--tRNA ligase, chloroplastic/mitochondrial [Cucumis melo]	[more]
XP_023524141.1	3.4e-266	77.44	serine--tRNA ligase, chloroplastic/mitochondrial isoform X1 [Cucurbita pepo subs...	[more]
XP_022940229.1	5.9e-266	77.60	serine--tRNA ligase, chloroplastic/mitochondrial isoform X1 [Cucurbita moschata]	[more]

BLAST of Lsi04G022480 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G11870.2	9.2e-209	63.82	Seryl-tRNA synthetase	[more]
AT1G11870.1	1.7e-207	63.82	Seryl-tRNA synthetase	[more]
AT1G11870.3	7.9e-144	57.64	Seryl-tRNA synthetase	[more]
AT5G27470.1	7.3e-57	40.75	seryl-tRNA synthetase / serine--tRNA ligase	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 171..205
None	No IPR available	COILS	Coil	Coil	coord: 134..161
None	No IPR available	GENE3D	3.30.930.10	Bira Bifunctional Protein; Domain 2	coord: 208..283 e-value: 1.1E-9 score: 39.8
None	No IPR available	GENE3D	3.30.930.10	Bira Bifunctional Protein; Domain 2	coord: 327..624 e-value: 1.2E-126 score: 424.3
None	No IPR available	PANTHER	PTHR11778:SF17	BNAA09G47500D PROTEIN	coord: 342..623
None	No IPR available	PANTHER	PTHR11778:SF17	BNAA09G47500D PROTEIN	coord: 67..261
None	No IPR available	SUPERFAMILY	55681	Class II aaRS and biotin synthetases	coord: 339..615
IPR002317	Serine-tRNA ligase, type1	PRINTS	PR00981	TRNASYNTHSER	coord: 449..461 score: 54.7 coord: 518..534 score: 45.1 coord: 536..552 score: 64.71 coord: 461..474 score: 59.79 coord: 501..514 score: 22.22
IPR002317	Serine-tRNA ligase, type1	TIGRFAM	TIGR00414	TIGR00414	coord: 336..614 e-value: 4.4E-104 score: 346.7
IPR002317	Serine-tRNA ligase, type1	PANTHER	PTHR11778	SERYL-TRNA SYNTHETASE	coord: 67..261
IPR002317	Serine-tRNA ligase, type1	PANTHER	PTHR11778	SERYL-TRNA SYNTHETASE	coord: 342..623
IPR002314	Aminoacyl-tRNA synthetase, class II (G/ P/ S/T)	PFAM	PF00587	tRNA-synt_2b	coord: 408..597 e-value: 2.7E-27 score: 95.9
IPR015866	Serine-tRNA synthetase, type1, N-terminal	PFAM	PF02403	Seryl_tRNA_N	coord: 106..209 e-value: 8.4E-20 score: 71.0
IPR042103	Serine-tRNA synthetase, type1, N-terminal domain superfamily	GENE3D	1.10.287.40		coord: 106..207 e-value: 5.8E-16 score: 60.6
IPR006195	Aminoacyl-tRNA synthetase, class II	PROSITE	PS50862	AA_TRNA_LIGASE_II	coord: 372..607 score: 22.773144
IPR033729	Serine-tRNA ligase catalytic core domain	CDD	cd00770	SerRS_core	coord: 223..614 e-value: 1.95795E-153 score: 441.999
IPR010978	Class I and II aminoacyl-tRNA synthetase, tRNA-binding arm	SUPERFAMILY	46589	tRNA-binding arm	coord: 106..215

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi04G022480.1	Lsi04G022480.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0070158	mitochondrial seryl-tRNA aminoacylation
biological_process	GO:0048481	plant ovule development
biological_process	GO:0006434	seryl-tRNA aminoacylation
biological_process	GO:0006418	tRNA aminoacylation for protein translation
cellular_component	GO:0009507	chloroplast
cellular_component	GO:0005739	mitochondrion
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0004828	serine-tRNA ligase activity
molecular_function	GO:0000049	tRNA binding
molecular_function	GO:0004812	aminoacyl-tRNA ligase activity
molecular_function	GO:0000166	nucleotide binding