Lsi04G022030 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi04G022030
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionCyclic nucleotide-gated ion channel like
Locationchr04: 29068095 .. 29072858 (+)
RNA-Seq ExpressionLsi04G022030
SyntenyLsi04G022030
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TTCATCTTCATCTTCCCCCAAAATGTGAACAAAATTTTGTAGCTGTCAATCAATGGATCAATCCCTCAATTCTTCCTTTTTTCCATTTCCAAATTTCCTTCTTCTTTATGCTCTACACGTTTTTCAAGATCCTCTCTCACGACTCAAACTCATTATTTTAATTTCAATTTCCACCATAAATTAACCCCAAATCTCTGTTTTCTTACTTTACTTCTTCTTCTTCTTCCTCCATTTTAACTCATCTCTGCCAGCTCTTTTCAGTGGGAAGTCAACCGCAAACGACAATCCCCCATCATTATTTCCCCGCGCTCTTTGCGGGATCCTTCTTTTTCTCCCAATGTGAATCTTTCCCACTTTTGTTCTTTGCTGAAATCGGTTCATGGAACTGAAGAAAGCCAAATTCGTCAGGTAATTTCCTGTAATTTCCTCTACTTTTTTTTTGAGATTTTGGTTGATGGGTTTTTCGTTTCTTTAGGTTTTACTCCAATGAGAAGAAGAATATTGAGCTTCCATGGGAGAAAAACGATGGGAAGAAGAGGAACGTTGAGTTCCCATTACCAGTTTTCAAAATGGGGAAGAAATTAGAGAATGAAAGTAAAATTATGAACTCAGATTCTTCAGGGAGGTCGAAGGTTTTTGTGGAGAGTAATGGGCCACAAGAAGAGAAGAATAAGAGGATTCTTAATCCAGAAAGTGAAAGGATTCTGAGATGGAATAGGGTTTTTCTCTTCTCTTGTTTGACAGCTCTTTTCGTTGACCCATTGTTCTTCTACCTTCCTTCAGTGATTCATCATAACAGGTCATCTTGTATGACTACTGATTTCAATTTGGGAATTGTTGTGACTGTTTTTCGTACTTTTGCCGATGTTTTCTATCTGTTGCATATGATTTTGAAGTTCCGGATTGCTTATGTATCACCAACTTCAAGAGTTTTTGGTAAAGGTGAACTCGTTACTGATCCAAAGAAGATTGCCAAACGCTATTTGAAATCAGATTTCTGTATAGATCTCATTGCTTCTCTGCCTCTTCCTCAGGTATTTTAGCACATTTGTTCATCACAGCTTCATGTTTGAAACAAGTTCCATTTGATAAGCATTTGGTTTTTGTAAATTCCAACTCTATTAGTATTTAGTTTCTTTGTTTTGTTATCTAATATTTATTAGTGTTTTCTAAATCAAAGCTACGTTTTCGAAAACTGAACAACGAAATTGTTAGTCAACGAAACTTATGTATTAGTTCATCACAACTTCTTTTTTGAAATTCAGCTTTTAATTTTAGGTACGTTTGTTTGATAATCATTTGTGCTCATATGATAACTATTAAATTTTTTATTTTATTTTTGAAAATTAAGTTGGTTTTCTCACATTTTTTTACAATGATATTCATCTTTTCTACGAAAATATATGGATTCTAAAACCGTTTTTAATTTTTTTTTTATAAAATGTACTTATTTTTAAAACTTGTCTTTGATTTTGAAAACATTCATAAAGATAGAGATAAGGAGAAATAAACAAATAAATAGAAGTAGTATTTATGAGCTTCATTTCCAAAAACAAAAAATCAAATATATCAAAAGGGACTTTGGTTTTTGGTTTTTAGTTTTTGAAAATTAAGCCTATAAATACTACGCTTCTTATTGATGAGTATTTTTAAAATCAAAGCCAAAATTTAAAAACAAACAAACAAAAAAAAAGTAGTTTGTTTTCGAAATTTAATTAAGAGTAAAGTTTAGTTAGAAAAGATGATAACTATTGTTAAGAAATTGTGAAAAATAAATATAATTTTCAAAAACAAAAAATCAGAAGTCGAATTAGTTATCAAAAAGGGGTTGTTCGTGTAAATTATATTGAAAAATCTGTTTGAGACCATGTTATCAATCAATTCTCAACTGTTATTTCAGCCTGTCCCAATATGATTGGCTTTGAAATTTGCAGTTTTTTTCATGTAGGCCCAGATTTGTGTGTGAGCTATACTGCAGCTGTTTCATATGAAGCAACAGTTTTCTATATTTTGTTTTTATATCTCAACTGAGGTCATAGATGATGTTATTTTTATTTCCACCTTTGATTTTTTTTTTTTTTTTTTTTTGTAAACTTATTTTGACAGATTATGATATGGTTTATCATGCCAGCAATTAGAAGCTCTCATGCAGACCATACAAACAATACTCTTGTGTTAATTGTCCTGCTTCAATATATTCCAAGATTTTACCTGATTTTCCCATTAAGTTCCCACATTATCAAAACTACTGGTGTTGTCACAAAGACTGCTTGGGCTGGGGCTGCATATAACCTTGTATTGTACATGCTAGCTAGTCATGTATGTAACAAACCCGAGCGCCTTTTGTCTCGTTTCTCGTTATTACATTCTAAATATACTTCAAAGGCAACCTGTTTGAAGTTTCTTTTGTTTACATAGATCTTAGGAGCAGCGTGGTATTTACTATCCGTGGAGCGCCATGCCATGTGCTGGAAGTTCGCCTGCAGGAACGAATTCAGTCCTATGAAATGTTTTCTTGACTATCTGGATTGTGGTACTTTAAATCATGTAGACCGCAGGATATGGGAAGTGAATACTACTGTATTTAGCCAATGTTCTCCAGATGAAGATGTCATTTTCAATTACGGGATATTTGCGAATGCGATCGCGAAAGATGTCATCTCCTCCAGGTTCCTTCAGAAGTATTTCTATTGTTTATGGTGGGGCTTACAGAACTTGAGGTATGAATACATTTGTGAGTAGTTGAGCTACTTGTATTTGTGTTTTTGCTTCTTAGATTGCTTTCTCAACTTTGACATGTTTCATTTATAAATTTTCCAGTTCCTATGGTCAGGGTTTGGAGACAAGCATCTACATGGGAGAGACTTTATTTGCTATCCTCATTGCTATAATGGGTTTGGTCTTGTTTGCACATTTGATCGGAAATATGCAGGTACATTCGAACTCATTTTGCATTTTGCACTGATCACCGTTCGATCTGTTGGAATCGGCTTAGAAAATACAGTAGTACTTTGCCTTTTCTGTATTTTTCTCATAGTTGTTACATAACATAATCACAATAAAGTTGCACTGGACCAAAGTGAATTTAACTCAACGGTAATTGATATGTACTCCATCCTTGAGGTCAAGATTCAATTCCCGTCCTCATAACCACTGGCACGGTTATACAATGACAAACCTATTGTGTTCAATGTTTATGTTCATGGCCTTTCAATTGACCATCTCCTTCATTTTGGAAGGCAATTATTTGAAAAGTGAAGACATCATTTTATGATAATATTTGTCATGCTTCTATAAACTTCTTAATCAACATGTCAACTTGGTTCTTATAGTTTGTCTTGCATTCATATATAGCATAAAGAGTATGAGTATGTTTTATCTTTGTTAGTTGTCTGGTTCCCCTCAAAATTTATTATTCTTTCTCTCTCTCTGAAACTTGTGTTATAGTGCAGACCTATTTGCAATCCATCACTGTGAGACTGGAGGAATGGAGGGTCAAGCGTCGAGACACTGAAGAATGGATGAAACATCGACAGCTCCCTCGAGATTTACAAGAACGTGTGAGGAGATTTGTTCAATATAAGTGGCTTGCAACACGAGGAGTCGACGAAGAGTCGATTCTACAAGGTCTTCCAACAGATCTCCGTCGAGATATTCAGCGCCACTTGTGTCTCGACCTTGTTCGACGTGTAAGCTCTGTTCTAAATCTCTTATTCTTATTTGTACCTCTCAGCCTGAAATGTTTGCTCTTTTGTTTTACATGCACATATCACATACACATGAACATGAAATATACTTGATCTATGAACAAAATTTGCTTTGTATGAAATCTACTTGTTTTTCGGGATTGCAGGTTCCTTTTTTCGCTCAAATGGATGATCAACTACTCGATGCAATTTGCGAGCGACTGGCTTCCTCTCTATGTACGCAACGTACTTACATTGTTCGTGAAGGAGATCCTGTTACTGAGATGCTCTTTATCATCCGAGGGATGCTCGAGAGTTCCACAACGGATGGAGGACGGTCAGGTTTTTTCAATTCGATAACCTTAAGACCTGGCGATTTTTGTGGCGAAGAGCTTCTGGCTTGGGCATTGCTACCAAAATCATCAATCAGCTTGCCATCTTCTACCAGAACAGTTCGAGCAATCACTGAAGTGGAAGCCTTTGCTCTACGTGCTGAGGATCTCAAATTCGTCGCTAACCAGTTTCGACGTCTTCATAGCAAGAAGCTTCAGCACACCTTCCGGTTTTATTCATATCACTGGAGAACCTGGGCAGCCTGCTTCATTCAGGCAGCATGGCGTCGCTTCAAAAGGCGAATCATAGCAAAGTCACTCAGCTTGCAGGAGTCTTTCTCGATGACTCCCGAGAAGCCTGTGGCCGAGGAAACCGAACAAGAAGAAGAAGAACATAACACTCCACGCTCCAATTACTCTCAAGCAAAACAAAACCTTGGTGTCACCATATTGGCTTCAAGGTTTGCTGCAAACACACGTAGAGGAGCACAAAAGCTTAAAGATATCAACTTATCCAAGCTGCGAAAGCCTGATGAGCCAGACTTTTCTGAAGAACCAGACGATTAACTTGTTGAATTTTTTTTTTTTTTTTTAAACCATTAAAGAACAGTCTCTTCTTTGACTATAATAAGATATTCACTTGTATATTATTACTTCTTTTTTGTTTTTCTACCTTTCATTAATTTATTTCTAACTTTGTATTTTGATATTGGAACTCAACCAATTCCTAGAGGTCTCTCAAACAA

mRNA sequence

TTCATCTTCATCTTCCCCCAAAATGTGAACAAAATTTTGTAGCTGTCAATCAATGGATCAATCCCTCAATTCTTCCTTTTTTCCATTTCCAAATTTCCTTCTTCTTTATGCTCTACACGTTTTTCAAGATCCTCTCTCACGACTCAAACTCATTATTTTAATTTCAATTTCCACCATAAATTAACCCCAAATCTCTGTTTTCTTACTTTACTTCTTCTTCTTCTTCCTCCATTTTAACTCATCTCTGCCAGCTCTTTTCAGTGGGAAGTCAACCGCAAACGACAATCCCCCATCATTATTTCCCCGCGCTCTTTGCGGGATCCTTCTTTTTCTCCCAATGTGAATCTTTCCCACTTTTGTTCTTTGCTGAAATCGGTTCATGGAACTGAAGAAAGCCAAATTCGTCAGGTTTTACTCCAATGAGAAGAAGAATATTGAGCTTCCATGGGAGAAAAACGATGGGAAGAAGAGGAACGTTGAGTTCCCATTACCAGTTTTCAAAATGGGGAAGAAATTAGAGAATGAAAGTAAAATTATGAACTCAGATTCTTCAGGGAGGTCGAAGGTTTTTGTGGAGAGTAATGGGCCACAAGAAGAGAAGAATAAGAGGATTCTTAATCCAGAAAGTGAAAGGATTCTGAGATGGAATAGGGTTTTTCTCTTCTCTTGTTTGACAGCTCTTTTCGTTGACCCATTGTTCTTCTACCTTCCTTCAGTGATTCATCATAACAGGTCATCTTGTATGACTACTGATTTCAATTTGGGAATTGTTGTGACTGTTTTTCGTACTTTTGCCGATGTTTTCTATCTGTTGCATATGATTTTGAAGTTCCGGATTGCTTATGTATCACCAACTTCAAGAGTTTTTGGTAAAGCAATTAGAAGCTCTCATGCAGACCATACAAACAATACTCTTGTGTTAATTGTCCTGCTTCAATATATTCCAAGATTTTACCTGATTTTCCCATTAAGTTCCCACATTATCAAAACTACTGGTGTTGTCACAAAGACTGCTTGGGCTGGGGCTGCATATAACCTTGTATTGTACATGCTAGCTAGTCATATCTTAGGAGCAGCGTGGTATTTACTATCCGTGGAGCGCCATGCCATGTGCTGGAAGTTCGCCTGCAGGAACGAATTCAGTCCTATGAAATGTTTTCTTGACTATCTGGATTGTGGTACTTTAAATCATGTAGACCGCAGGATATGGGAAGTGAATACTACTGTATTTAGCCAATGTTCTCCAGATGAAGATGTCATTTTCAATTACGGGATATTTGCGAATGCGATCGCGAAAGATGTCATCTCCTCCAGGTTCCTTCAGAAGTATTTCTATTGTTTATGGTGGGGCTTACAGAACTTGAGGTATGAATACATTTGTGATTCCTATGGTCAGGGTTTGGAGACAAGCATCTACATGGGAGAGACTTTATTTGCTATCCTCATTGCTATAATGGGTTTGGTCTTGTTTGCACATTTGATCGGAAATATGCAGACCTATTTGCAATCCATCACTGTGAGACTGGAGGAATGGAGGGTCAAGCGTCGAGACACTGAAGAATGGATGAAACATCGACAGCTCCCTCGAGATTTACAAGAACGTGTGAGGAGATTTGTTCAATATAAGTGGCTTGCAACACGAGGAGTCGACGAAGAGTCGATTCTACAAGGTCTTCCAACAGATCTCCGTCGAGATATTCAGCGCCACTTGTGTCTCGACCTTGTTCGACGTGTTCCTTTTTTCGCTCAAATGGATGATCAACTACTCGATGCAATTTGCGAGCGACTGGCTTCCTCTCTATGTACGCAACGTACTTACATTGTTCGTGAAGGAGATCCTGTTACTGAGATGCTCTTTATCATCCGAGGGATGCTCGAGAGTTCCACAACGGATGGAGGACGGTCAGGTTTTTTCAATTCGATAACCTTAAGACCTGGCGATTTTTGTGGCGAAGAGCTTCTGGCTTGGGCATTGCTACCAAAATCATCAATCAGCTTGCCATCTTCTACCAGAACAGTTCGAGCAATCACTGAAGTGGAAGCCTTTGCTCTACGTGCTGAGGATCTCAAATTCGTCGCTAACCAGTTTCGACGTCTTCATAGCAAGAAGCTTCAGCACACCTTCCGGTTTTATTCATATCACTGGAGAACCTGGGCAGCCTGCTTCATTCAGGCAGCATGGCGTCGCTTCAAAAGGCGAATCATAGCAAAGTCACTCAGCTTGCAGGAGTCTTTCTCGATGACTCCCGAGAAGCCTGTGGCCGAGGAAACCGAACAAGAAGAAGAAGAACATAACACTCCACGCTCCAATTACTCTCAAGCAAAACAAAACCTTGGTGTCACCATATTGGCTTCAAGGTTTGCTGCAAACACACGTAGAGGAGCACAAAAGCTTAAAGATATCAACTTATCCAAGCTGCGAAAGCCTGATGAGCCAGACTTTTCTGAAGAACCAGACGATTAACTTGTTGAATTTTTTTTTTTTTTTTTAAACCATTAAAGAACAGTCTCTTCTTTGACTATAATAAGATATTCACTTGTATATTATTACTTCTTTTTTGTTTTTCTACCTTTCATTAATTTATTTCTAACTTTGTATTTTGATATTGGAACTCAACCAATTCCTAGAGGTCTCTCAAACAA

Coding sequence (CDS)

ATGGAACTGAAGAAAGCCAAATTCGTCAGGTTTTACTCCAATGAGAAGAAGAATATTGAGCTTCCATGGGAGAAAAACGATGGGAAGAAGAGGAACGTTGAGTTCCCATTACCAGTTTTCAAAATGGGGAAGAAATTAGAGAATGAAAGTAAAATTATGAACTCAGATTCTTCAGGGAGGTCGAAGGTTTTTGTGGAGAGTAATGGGCCACAAGAAGAGAAGAATAAGAGGATTCTTAATCCAGAAAGTGAAAGGATTCTGAGATGGAATAGGGTTTTTCTCTTCTCTTGTTTGACAGCTCTTTTCGTTGACCCATTGTTCTTCTACCTTCCTTCAGTGATTCATCATAACAGGTCATCTTGTATGACTACTGATTTCAATTTGGGAATTGTTGTGACTGTTTTTCGTACTTTTGCCGATGTTTTCTATCTGTTGCATATGATTTTGAAGTTCCGGATTGCTTATGTATCACCAACTTCAAGAGTTTTTGGTAAAGCAATTAGAAGCTCTCATGCAGACCATACAAACAATACTCTTGTGTTAATTGTCCTGCTTCAATATATTCCAAGATTTTACCTGATTTTCCCATTAAGTTCCCACATTATCAAAACTACTGGTGTTGTCACAAAGACTGCTTGGGCTGGGGCTGCATATAACCTTGTATTGTACATGCTAGCTAGTCATATCTTAGGAGCAGCGTGGTATTTACTATCCGTGGAGCGCCATGCCATGTGCTGGAAGTTCGCCTGCAGGAACGAATTCAGTCCTATGAAATGTTTTCTTGACTATCTGGATTGTGGTACTTTAAATCATGTAGACCGCAGGATATGGGAAGTGAATACTACTGTATTTAGCCAATGTTCTCCAGATGAAGATGTCATTTTCAATTACGGGATATTTGCGAATGCGATCGCGAAAGATGTCATCTCCTCCAGGTTCCTTCAGAAGTATTTCTATTGTTTATGGTGGGGCTTACAGAACTTGAGGTATGAATACATTTGTGATTCCTATGGTCAGGGTTTGGAGACAAGCATCTACATGGGAGAGACTTTATTTGCTATCCTCATTGCTATAATGGGTTTGGTCTTGTTTGCACATTTGATCGGAAATATGCAGACCTATTTGCAATCCATCACTGTGAGACTGGAGGAATGGAGGGTCAAGCGTCGAGACACTGAAGAATGGATGAAACATCGACAGCTCCCTCGAGATTTACAAGAACGTGTGAGGAGATTTGTTCAATATAAGTGGCTTGCAACACGAGGAGTCGACGAAGAGTCGATTCTACAAGGTCTTCCAACAGATCTCCGTCGAGATATTCAGCGCCACTTGTGTCTCGACCTTGTTCGACGTGTTCCTTTTTTCGCTCAAATGGATGATCAACTACTCGATGCAATTTGCGAGCGACTGGCTTCCTCTCTATGTACGCAACGTACTTACATTGTTCGTGAAGGAGATCCTGTTACTGAGATGCTCTTTATCATCCGAGGGATGCTCGAGAGTTCCACAACGGATGGAGGACGGTCAGGTTTTTTCAATTCGATAACCTTAAGACCTGGCGATTTTTGTGGCGAAGAGCTTCTGGCTTGGGCATTGCTACCAAAATCATCAATCAGCTTGCCATCTTCTACCAGAACAGTTCGAGCAATCACTGAAGTGGAAGCCTTTGCTCTACGTGCTGAGGATCTCAAATTCGTCGCTAACCAGTTTCGACGTCTTCATAGCAAGAAGCTTCAGCACACCTTCCGGTTTTATTCATATCACTGGAGAACCTGGGCAGCCTGCTTCATTCAGGCAGCATGGCGTCGCTTCAAAAGGCGAATCATAGCAAAGTCACTCAGCTTGCAGGAGTCTTTCTCGATGACTCCCGAGAAGCCTGTGGCCGAGGAAACCGAACAAGAAGAAGAAGAACATAACACTCCACGCTCCAATTACTCTCAAGCAAAACAAAACCTTGGTGTCACCATATTGGCTTCAAGGTTTGCTGCAAACACACGTAGAGGAGCACAAAAGCTTAAAGATATCAACTTATCCAAGCTGCGAAAGCCTGATGAGCCAGACTTTTCTGAAGAACCAGACGATTAA

Protein sequence

MELKKAKFVRFYSNEKKNIELPWEKNDGKKRNVEFPLPVFKMGKKLENESKIMNSDSSGRSKVFVESNGPQEEKNKRILNPESERILRWNRVFLFSCLTALFVDPLFFYLPSVIHHNRSSCMTTDFNLGIVVTVFRTFADVFYLLHMILKFRIAYVSPTSRVFGKAIRSSHADHTNNTLVLIVLLQYIPRFYLIFPLSSHIIKTTGVVTKTAWAGAAYNLVLYMLASHILGAAWYLLSVERHAMCWKFACRNEFSPMKCFLDYLDCGTLNHVDRRIWEVNTTVFSQCSPDEDVIFNYGIFANAIAKDVISSRFLQKYFYCLWWGLQNLRYEYICDSYGQGLETSIYMGETLFAILIAIMGLVLFAHLIGNMQTYLQSITVRLEEWRVKRRDTEEWMKHRQLPRDLQERVRRFVQYKWLATRGVDEESILQGLPTDLRRDIQRHLCLDLVRRVPFFAQMDDQLLDAICERLASSLCTQRTYIVREGDPVTEMLFIIRGMLESSTTDGGRSGFFNSITLRPGDFCGEELLAWALLPKSSISLPSSTRTVRAITEVEAFALRAEDLKFVANQFRRLHSKKLQHTFRFYSYHWRTWAACFIQAAWRRFKRRIIAKSLSLQESFSMTPEKPVAEETEQEEEEHNTPRSNYSQAKQNLGVTILASRFAANTRRGAQKLKDINLSKLRKPDEPDFSEEPDD
Homology
BLAST of Lsi04G022030 vs. ExPASy Swiss-Prot
Match: Q9SJA4 (Probable cyclic nucleotide-gated ion channel 14 OS=Arabidopsis thaliana OX=3702 GN=CNGC14 PE=2 SV=2)

HSP 1 Score: 865.9 bits (2236), Expect = 3.1e-250
Identity = 469/746 (62.87%), Postives = 541/746 (72.52%), Query Frame = 0

Query: 1   MELKKAKFVRFYSNEKKNIELPWEKNDGKKRNVEFPLPVFK-----------MGKKLENE 60
           ME K+   VRFY +EK+ IE+      G+KR     +P+FK           + KK +  
Sbjct: 1   MEFKRDNTVRFYGDEKQTIEV------GEKR-----VPLFKSTTAPFMKQEVLPKKSKTR 60

Query: 61  SKIMNSDSSGRSKVFVESNGPQEEKNKRILNPESERILRWNRVFLFSCLTALFVDPLFFY 120
            KI      GR KVF E+    E +  +IL+P  + +L+WNRVFLF CL AL+VDPLFF+
Sbjct: 61  LKI---PRFGRFKVFPEN---FEIERDKILDPGGDAVLQWNRVFLFWCLVALYVDPLFFF 120

Query: 121 LPSVIHHNRSSCMTTDFNLGIVVTVFRTFADVFYLLHMILKFRIAYVSPTSRVFGK---- 180
           L SV    RSSCMTTD  LGIV+T FRT AD+FY+LH+++KFR AYVS TSRVFG+    
Sbjct: 121 LSSVKRIGRSSCMTTDLKLGIVITFFRTLADLFYVLHIVIKFRTAYVSRTSRVFGRGELV 180

Query: 181 -----------------------------------AIRSSHADHTNNTLVLIVLLQYIPR 240
                                              +IRSSH+DHT N LVLIVL+QYIPR
Sbjct: 181 KDPKLIARRYLRSDFIVDLIACLPLPQIVSWFILPSIRSSHSDHTTNALVLIVLVQYIPR 240

Query: 241 FYLIFPLSSHIIKTTGVVTKTAWAGAAYNLVLYMLASHILGAAWYLLSVERHAMCWKFAC 300
            YLIFPLS+ IIK TGVVT TAWAGAAYNL+ YMLASHILG+AWYLLS+ER A CWK  C
Sbjct: 241 LYLIFPLSAEIIKATGVVTTTAWAGAAYNLLQYMLASHILGSAWYLLSIERQATCWKAEC 300

Query: 301 RNEFSPMKCFLDYLDCGTLNHVDRRIWEVNTTVFSQCSPDEDVIFNYGIFANAIAKDVIS 360
             E  P++C  D+ DCGTL+  DR  W+  T VFS C P  ++ F +GIFA+A+ K+V+S
Sbjct: 301 HKESVPLQCVTDFFDCGTLHRDDRNNWQNTTVVFSNCDPSNNIQFTFGIFADALTKNVVS 360

Query: 361 SRFLQKYFYCLWWGLQNLRYEYICDSYGQGLETSIYMGETLFAILIAIMGLVLFAHLIGN 420
           S FL+KY YCLW+GLQNL       SYGQ L TS  + ET+FAIL+AI GLVLFA LIGN
Sbjct: 361 SPFLEKYLYCLWFGLQNL------SSYGQNLSTSTSVLETMFAILVAIFGLVLFALLIGN 420

Query: 421 MQTYLQSITVRLEEWRVKRRDTEEWMKHRQLPRDLQERVRRFVQYKWLATRGVDEESILQ 480
           MQTYLQSITVRLEEWR+KRRDTEEWM HR LP++L+ERVRRFVQYKWLATRGVDEE+IL 
Sbjct: 421 MQTYLQSITVRLEEWRLKRRDTEEWMGHRLLPQNLRERVRRFVQYKWLATRGVDEETILH 480

Query: 481 GLPTDLRRDIQRHLCLDLVRRVPFFAQMDDQLLDAICERLASSLCTQRTYIVREGDPVTE 540
            LP DLRRDIQRHLCLDLVRRVP FAQMDDQLLDAICERLASSL TQ  YIVREGDPVTE
Sbjct: 481 SLPADLRRDIQRHLCLDLVRRVPLFAQMDDQLLDAICERLASSLSTQGNYIVREGDPVTE 540

Query: 541 MLFIIRGMLESSTTDGGRSGFFNSITLRPGDFCGEELLAWALLPKSSISLPSSTRTVRAI 600
           MLFIIRG LESSTT+GGR+GFFNSITLRPGDFCGEELLAWALLPKS+++LPSSTRTVRA+
Sbjct: 541 MLFIIRGKLESSTTNGGRTGFFNSITLRPGDFCGEELLAWALLPKSTVNLPSSTRTVRAL 600

Query: 601 TEVEAFALRAEDLKFVANQFRRLHSKKLQHTFRFYSYHWRTWAACFIQAAWRRFKRRIIA 660
            EVEAFAL+A DLKFVANQFRRLHSKKLQHTFR+YS+ WRTWAACF+Q AWRR+KR+ +A
Sbjct: 601 EEVEAFALQAGDLKFVANQFRRLHSKKLQHTFRYYSHQWRTWAACFVQVAWRRYKRKKLA 660

Query: 661 KSLSLQESFSMTPEKPVAEETEQEEEEHN-------TPRSNYSQAKQNLGVTILASRFAA 690
           KSLSL ESFS   E+        EE  H          R + S  K +   TILASRFA 
Sbjct: 661 KSLSLAESFSSYDEEEAVAVAATEEMSHEGEAQSGAKARHHTSNVKPHFAATILASRFAK 720

BLAST of Lsi04G022030 vs. ExPASy Swiss-Prot
Match: Q8L7Z0 (Cyclic nucleotide-gated ion channel 17 OS=Arabidopsis thaliana OX=3702 GN=CNGC17 PE=1 SV=1)

HSP 1 Score: 826.6 bits (2134), Expect = 2.1e-238
Identity = 441/737 (59.84%), Postives = 531/737 (72.05%), Query Frame = 0

Query: 1   MELKKAKFVRFYSNEKKNIELPWEKNDGKKRNVEFPLPVFKMGKKLENESKIMNSDSSGR 60
           MEL+K K + FYS  K++ E  W  ND   ++ +  LP       L   +++  +D+S  
Sbjct: 1   MELRKDKLLMFYSEGKESKEAKWAVNDPMSKSYKLSLPSALRPDNLLPGNRLRYTDAS-- 60

Query: 61  SKVFVESNGPQEEKNKRILNPESERILRWNRVFLFSCLTALFVDPLFFYLPSVIHHNRSS 120
                +S   +    K IL+P SE +L+WN VF+ SC+ ALF+DPL+F++P++       
Sbjct: 61  -----KSKSSKVSWYKTILDPGSEIVLKWNWVFIVSCMVALFIDPLYFFVPAIGGDKNYP 120

Query: 121 CMTTDFNLGIVVTVFRTFADVFYLLHMILKFRIAYVSPTS--RVFG--------KAI--- 180
           C  TD +L I+VT FRT AD+FYLLH+ +KFR  +++P S  RVFG        KAI   
Sbjct: 121 CARTDTSLSILVTFFRTIADLFYLLHIFIKFRTGFIAPNSSTRVFGRGELVMDPKAIAWR 180

Query: 181 ----------------------------RSSHADHTNNTLVLIVLLQYIPRFYLIFPLSS 240
                                       +S   DH NN + LIVLLQYIPRFYLI PLSS
Sbjct: 181 YIKSDFIIDLIATLPLPQIVIWFVISTTKSYRFDHNNNAIALIVLLQYIPRFYLIIPLSS 240

Query: 241 HIIKTTGVVTKTAWAGAAYNLVLYMLASHILGAAWYLLSVERHAMCWKFACRNEFSPMKC 300
            I+K TGVVTKTAWAGAAYNL+LYMLASH+LGAAWY+LSV+R+  CWK  C  E   + C
Sbjct: 241 QIVKATGVVTKTAWAGAAYNLLLYMLASHVLGAAWYILSVDRYTSCWKSRCNGEAGQVNC 300

Query: 301 FLDYLDCGTLNHVDRRIWEVNTTVFSQCSPDEDVIFNYGIFANAIAKDVISSRFLQKYFY 360
            L YLDC ++   ++  W   T VF  C    +  F YGIF NAI K+V+SS+F ++YFY
Sbjct: 301 QLYYLDCDSMYDNNQMTWANVTKVFKLCDA-RNGEFKYGIFGNAITKNVVSSQFFERYFY 360

Query: 361 CLWWGLQNLRYEYICDSYGQGLETSIYMGETLFAILIAIMGLVLFAHLIGNMQTYLQSIT 420
           CLWWGLQ L       SYGQ L T+++MGET FA+LIAI GLVLFAHLIGNMQTYLQS+T
Sbjct: 361 CLWWGLQQL------SSYGQNLSTTMFMGETTFAVLIAIFGLVLFAHLIGNMQTYLQSLT 420

Query: 421 VRLEEWRVKRRDTEEWMKHRQLPRDLQERVRRFVQYKWLATRGVDEESILQGLPTDLRRD 480
           VRLEEWR+K+RDTEEWM+HRQLP +L+ RVRR+ QYKWLATRGVDEE +LQ LPTDLRRD
Sbjct: 421 VRLEEWRLKKRDTEEWMRHRQLPEELRNRVRRYEQYKWLATRGVDEEVLLQSLPTDLRRD 480

Query: 481 IQRHLCLDLVRRVPFFAQMDDQLLDAICERLASSLCTQRTYIVREGDPVTEMLFIIRGML 540
           IQRHLCLDLVRRVPFF+QMDDQLLDAICERL SSLCT+ TY+VREGD ++EMLFIIRG L
Sbjct: 481 IQRHLCLDLVRRVPFFSQMDDQLLDAICERLVSSLCTEGTYLVREGDLISEMLFIIRGRL 540

Query: 541 ESSTTDGGRSGFFNSITLRPGDFCGEELLAWALLPKSSISLPSSTRTVRAITEVEAFALR 600
           ESSTT+GGR+GFFNSI LRPGDFCGEELL+WALLPKS+++LPSSTRTVRA+ EVEAFALR
Sbjct: 541 ESSTTNGGRTGFFNSIILRPGDFCGEELLSWALLPKSTLNLPSSTRTVRALVEVEAFALR 600

Query: 601 AEDLKFVANQFRRLHSKKLQHTFRFYSYHWRTWAACFIQAAWRRFKRRIIAKSLSLQESF 660
           AEDLKFVANQFRRLHSKKLQHTFRFYS+HWRTWAACFIQAAWRR+KRR++  +L+  ES 
Sbjct: 601 AEDLKFVANQFRRLHSKKLQHTFRFYSHHWRTWAACFIQAAWRRYKRRVMENNLTAIESM 660

Query: 661 SMTPEKPVAEETEQEEEEHNTPRSNYSQAKQNLGVTILASRFAANTRRG--AQKLKDINL 695
               E  V EE    EEE     S   + K NLGV +LASRFAANTRRG  AQ++KD+ L
Sbjct: 661 E-NEEGEVGEELVVVEEEECVEES--PRTKMNLGVMVLASRFAANTRRGVAAQRVKDVEL 720

BLAST of Lsi04G022030 vs. ExPASy Swiss-Prot
Match: Q9LEQ3 (Cyclic nucleotide-gated ion channel 18 OS=Arabidopsis thaliana OX=3702 GN=CNGC18 PE=1 SV=1)

HSP 1 Score: 709.5 bits (1830), Expect = 3.7e-203
Identity = 377/676 (55.77%), Postives = 466/676 (68.93%), Query Frame = 0

Query: 77  RILNPESERILRWNRVFLFSCLTALFVDPLFFYLPSVIHHNRSSCMTTDFNLGIVVTVFR 136
           +IL+P+S  +  WN VFL + + ALF+DP +FY+P V      +C++ D +L   VT FR
Sbjct: 39  QILDPDSNIVTYWNHVFLITSILALFLDPFYFYVPYV---GGPACLSIDISLAATVTFFR 98

Query: 137 TFADVFYLLHMILKFRIAYVSPTSRVFGK------------------------------- 196
           T AD+F+LLH+ +KFR A+V+ +SRVFG+                               
Sbjct: 99  TVADIFHLLHIFMKFRTAFVARSSRVFGRGELVMDSREIAMRYLKTDFLIDVAAMLPLPQ 158

Query: 197 --------AIRSSHADHTNNTLVLIVLLQYIPRFYLIFPLSSHIIKTTGVVTKTAWAGAA 256
                   A  +  A+H N+TL LIVL+QYIPR ++IFPL+  IIKTTG + KTAWAGAA
Sbjct: 159 LVIWLVIPAATNGTANHANSTLALIVLVQYIPRSFIIFPLNQRIIKTTGFIAKTAWAGAA 218

Query: 257 YNLVLYMLASHILGAAWYLLSVERHAMCWKFACR--NEFSPMKCFLDYLDCGTLNHVDRR 316
           YNL+LY+LASH+LGA WYL S+ R   CW   C+  N    + C   +LDC +L   +R+
Sbjct: 219 YNLLLYILASHVLGAMWYLSSIGRQFSCWSNVCKKDNALRVLDCLPSFLDCKSLEQPERQ 278

Query: 317 IWEVNTTVFSQCSPDEDVI-FNYGIFANAIAKDVISSRFLQKYFYCLWWGLQNLRYEYIC 376
            W+  T V S C        F +G+FA A    V ++ F+ KY YCLWWGL+NL      
Sbjct: 279 YWQNVTQVLSHCDATSSTTNFKFGMFAEAFTTQVATTDFVSKYLYCLWWGLRNL------ 338

Query: 377 DSYGQGLETSIYMGETLFAILIAIMGLVLFAHLIGNMQTYLQSITVRLEEWRVKRRDTEE 436
            SYGQ + TS+Y+GETLF I I I GL+LF  LIGNMQ+ LQS++VR+EEWRVKRRDTEE
Sbjct: 339 SSYGQNITTSVYLGETLFCITICIFGLILFTLLIGNMQSSLQSMSVRVEEWRVKRRDTEE 398

Query: 437 WMKHRQLPRDLQERVRRFVQYKWLATRGVDEESILQGLPTDLRRDIQRHLCLDLVRRVPF 496
           WM+HRQLP +LQERVRRFVQYKWLATRGVDEESIL  LPTDLRR+IQRHLCL LVRRVPF
Sbjct: 399 WMRHRQLPPELQERVRRFVQYKWLATRGVDEESILHSLPTDLRREIQRHLCLSLVRRVPF 458

Query: 497 FAQMDDQLLDAICERLASSLCTQRTYIVREGDPVTEMLFIIRGMLESSTTDGGRSGFFNS 556
           F+QMDDQLLDAIC  L SSL T  TYI REGDPV EMLF+IRG +ESSTT+GGRSGFFNS
Sbjct: 459 FSQMDDQLLDAICGCLVSSLSTAGTYIFREGDPVNEMLFVIRGQIESSTTNGGRSGFFNS 518

Query: 557 ITLRPGDFCGEELLAWALLPKSSISLPSSTRTVRAITEVEAFALRAEDLKFVANQFRRLH 616
            TLRPGDFCGEELL WAL+P S+++LPSSTR+VRA++EVEAFAL AEDLKFVA+QF+RL 
Sbjct: 519 TTLRPGDFCGEELLTWALMPNSTLNLPSSTRSVRALSEVEAFALSAEDLKFVAHQFKRLQ 578

Query: 617 SKKLQHTFRFYSYHWRTWAACFIQAAWRRFKRRIIAKSLSLQESFS-MTPEKPVAEETEQ 676
           SKKLQH FR+YS+ WR W ACF+Q+AWRR+KRR +AK LSL ES     P++    E ++
Sbjct: 579 SKKLQHAFRYYSHQWRAWGACFVQSAWRRYKRRKLAKELSLHESSGYYYPDETGYNEEDE 638

Query: 677 EEEE--HNTPRSNYSQAKQNLGVTILASRFAANTRRGAQK--------LKD-----INLS 695
           E  E  + +     S    NLG TILAS+FAANTRRG  +         KD     + + 
Sbjct: 639 ETREYYYGSDEEGGSMDNTNLGATILASKFAANTRRGTNQKASSSSTGKKDGSSTSLKMP 698

BLAST of Lsi04G022030 vs. ExPASy Swiss-Prot
Match: Q9SU64 (Probable cyclic nucleotide-gated ion channel 16 OS=Arabidopsis thaliana OX=3702 GN=CNGC16 PE=2 SV=1)

HSP 1 Score: 693.7 bits (1789), Expect = 2.1e-198
Identity = 373/688 (54.22%), Postives = 457/688 (66.42%), Query Frame = 0

Query: 68  NGPQEEKNKRI--------LNPESERILRWNRVFLFSCLTALFVDPLFFYLPSVIHHNRS 127
           N P  ++ + I        L+P  + I RWN +FL +CL ALF+DPL+FYLP V     +
Sbjct: 26  NDPNNQRRRSIFSKLRDKTLDPGGDLITRWNHIFLITCLLALFLDPLYFYLPIV--QAGT 85

Query: 128 SCMTTDFNLGIVVTVFRTFADVFYLLHMILKFRIAYVSPTSRVFGK----------AIR- 187
           +CM+ D   GI VT FR  AD+ +L+H++LKF+ A+VS +SRVFG+          AIR 
Sbjct: 86  ACMSIDVRFGIFVTCFRNLADLSFLIHILLKFKTAFVSKSSRVFGRGELVMDRREIAIRY 145

Query: 188 -----------------------------SSHADHTNNTLVLIVLLQYIPRFYLIFPLSS 247
                                          +A H N+TL LIVL+QY+PRF ++ PL+ 
Sbjct: 146 LKSEFVIDLAATLPLPQIMIWFVIPNAGEFRYAAHQNHTLSLIVLIQYVPRFLVMLPLNR 205

Query: 248 HIIKTTGVVTKTAWAGAAYNLVLYMLASHILGAAWYLLSVERHAMCWKFACRNEFSPM-- 307
            IIK TGV  KTAW+GAAYNL+LY+L SH+LG+ WY+LS++R   CW+  C  E +    
Sbjct: 206 RIIKATGVAAKTAWSGAAYNLILYLLVSHVLGSVWYVLSIQRQHECWRRECIKEMNATHS 265

Query: 308 -KCFLDYLDCGTLNHVDRRIWEVNTTVFSQCSP--DEDVIFNYGIFANAIAKDVISSRFL 367
             C L +LDCG+L+   R+ W   T V S C    D+D  F +G+F +A   DV SS F 
Sbjct: 266 PSCSLLFLDCGSLHDPGRQAWMRITRVLSNCDARNDDDQHFQFGMFGDAFTNDVTSSPFF 325

Query: 368 QKYFYCLWWGLQNLRYEYICDSYGQGLETSIYMGETLFAILIAIMGLVLFAHLIGNMQTY 427
            KYFYCLWWGL+NL       SYGQ L  S    ET+F+  I + GLV F+HLIGN+Q Y
Sbjct: 326 DKYFYCLWWGLRNL------SSYGQSLAASTLSSETIFSCFICVAGLVFFSHLIGNVQNY 385

Query: 428 LQSITVRLEEWRVKRRDTEEWMKHRQLPRDLQERVRRFVQYKWLATRGVDEESILQGLPT 487
           LQS T RL+EWRV+RRDTEEWM+HRQLP +LQERVRRFVQYKWL TRGVDEE+IL+ LP 
Sbjct: 386 LQSTTARLDEWRVRRRDTEEWMRHRQLPDELQERVRRFVQYKWLTTRGVDEEAILRALPL 445

Query: 488 DLRRDIQRHLCLDLVRRVPFFAQMDDQLLDAICERLASSLCTQRTYIVREGDPVTEMLFI 547
           DLRR IQRHLCL LVRRVPFFAQMDDQLLDAICERL  SL T+ TY++REGDPV EMLFI
Sbjct: 446 DLRRQIQRHLCLALVRRVPFFAQMDDQLLDAICERLVPSLNTKDTYVIREGDPVNEMLFI 505

Query: 548 IRGMLESSTTDGGRSGFFNSITLRPGDFCGEELLAWALLPKSSISLPSSTRTVRAITEVE 607
           IRG +ESSTTDGGRSGFFNSITLRPGDFCGEELL WAL+P  + +LP STRTVR ++EVE
Sbjct: 506 IRGQMESSTTDGGRSGFFNSITLRPGDFCGEELLTWALVPNINHNLPLSTRTVRTLSEVE 565

Query: 608 AFALRAEDLKFVANQFRRLHSKKLQHTFRFYSYHWRTWAACFIQAAWRRFKRRIIAKSLS 667
           AFALRAEDLKFVANQFRRLHSKKLQH FR+YS+ WR W  CFIQAAWRR+ +R +A  L+
Sbjct: 566 AFALRAEDLKFVANQFRRLHSKKLQHAFRYYSHQWRAWGTCFIQAAWRRYMKRKLAMELA 625

Query: 668 LQE---SFSMTPEKPVAEETEQEEEEHNTPRSNYSQAKQNLGVTILASRFAANTRRGA-- 689
            QE    +    +     E +  E  +N    N S   QNL  TILAS+FAANT+RG   
Sbjct: 626 RQEEEDDYFYDDDGDYQFEEDMPESNNNNGDEN-SSNNQNLSATILASKFAANTKRGVLG 685

BLAST of Lsi04G022030 vs. ExPASy Swiss-Prot
Match: A0A072VMJ3 (Protein CNGC15c OS=Medicago truncatula OX=3880 GN=CNGC15C PE=1 SV=1)

HSP 1 Score: 664.8 bits (1714), Expect = 1.0e-189
Identity = 362/697 (51.94%), Postives = 461/697 (66.14%), Query Frame = 0

Query: 37  LPVFKMGKKLENESKIMNSDSSGRSKVFVESNGPQEEKNKRILNPESERILRWNRVFLFS 96
           +P     K  +NE++     +   S+VF E +  + +K   +L+P  + I RWN++FL +
Sbjct: 37  IPEQSSKKSRKNETRNKFLKTRVLSRVFSE-DYERVKKRVLVLDPRGQLIHRWNKIFLVA 96

Query: 97  CLTALFVDPLFFYLPSVIHHNRSSCMTTDFNLGIVVTVFRTFADVFYLLHMILKFRIAYV 156
           CL +LFVDPLFFYLP V       C+     L +++TV R+F D+FY++ + +KFR AYV
Sbjct: 97  CLVSLFVDPLFFYLPVV---REEVCIDIGKTLEVILTVVRSFGDLFYIVQICMKFRTAYV 156

Query: 157 SPTSRVFGK---------------------------------------AIRSSHADHTNN 216
           +P+S+VFG+                                        +R S   +T N
Sbjct: 157 APSSKVFGRGELVLTYSKIALRYFSKGFWLDFIAALPLPQVLIWIIIPTLRGSTMANTKN 216

Query: 217 TLVLIVLLQYIPRFYLIFPLSSHIIKTTGVVTKTAWAGAAYNLVLYMLASHILGAAWYLL 276
            L   ++ QYIPR YLIFPLSS I+K TGVVT+TAWAGAAYNL+LYMLASHILGA WYLL
Sbjct: 217 VLRFFIIFQYIPRLYLIFPLSSQIVKATGVVTETAWAGAAYNLMLYMLASHILGACWYLL 276

Query: 277 SVERHAMCWKFACRNEFSPMKCFLDYLDCGTLNHVDRRIWEVNTTVFSQCSPDEDVIFNY 336
           S+ER   CWK  C  E S   C   + +C ++    R  W + + V + CSP+    + +
Sbjct: 277 SIERQEACWKSVCNMEKS--NCQYGFFNCHSIKDAPRVAWFIASNVTNLCSPNAG-FYPF 336

Query: 337 GIFANAIAKDVISSRFLQKYFYCLWWGLQNLRYEYICDSYGQGLETSIYMGETLFAILIA 396
           GI+A+A+   V SS F  KYFYCLWWGL+NL       S GQGL TS ++GE + AI++A
Sbjct: 337 GIYADAMTSKVTSSPFFNKYFYCLWWGLRNL------SSLGQGLLTSTFIGEIMVAIVVA 396

Query: 397 IMGLVLFAHLIGNMQTYLQSITVRLEEWRVKRRDTEEWMKHRQLPRDLQERVRRFVQYKW 456
            +GLVLFA LIGNMQTYLQSITVRLEEWRVKR DTE+WM HRQLP +L+E +R++ QYKW
Sbjct: 397 TLGLVLFALLIGNMQTYLQSITVRLEEWRVKRTDTEQWMHHRQLPPELRESIRKYNQYKW 456

Query: 457 LATRGVDEESILQGLPTDLRRDIQRHLCLDLVRRVPFFAQMDDQLLDAICERLASSLCTQ 516
           +ATRGV+EE +L+GLP DLRR+I+RHLCL+LVR VP F QMD+++LDAICERL  +LCT+
Sbjct: 457 VATRGVEEEDLLKGLPLDLRREIKRHLCLELVRGVPLFDQMDERMLDAICERLKPALCTE 516

Query: 517 RTYIVREGDPVTEMLFIIRGMLESSTTDGGRSGFFNSITLRPGDFCGEELLAWALLPKSS 576
            TY+VREGDPV EMLFIIRG L+S TT+GGR GFFNS  + PGDFCGEELL WAL P+ S
Sbjct: 517 GTYLVREGDPVNEMLFIIRGHLDSYTTNGGRDGFFNSCRIGPGDFCGEELLTWALDPRPS 576

Query: 577 ISLPSSTRTVRAITEVEAFALRAEDLKFVANQFRRLHSKKLQHTFRFYSYHWRTWAACFI 636
           + LPSSTRTV+A +EVEAFAL AEDLKFVA+QFRRLHSK+L+H FRFYS+ WRTWAACFI
Sbjct: 577 VILPSSTRTVKAFSEVEAFALIAEDLKFVASQFRRLHSKQLRHKFRFYSHQWRTWAACFI 636

Query: 637 QAAWRRFKRRIIAKSLSLQESFSMTPEKPVAEETEQEEEEHNTPRSNYSQAKQNLGVTIL 692
           QAAWRR K+R  A  L  +E+         A E E E  +           K   G  + 
Sbjct: 637 QAAWRRHKKRKEAAELRAKENL------VAASEAENEIAK-----------KYGKGFVVY 696

BLAST of Lsi04G022030 vs. ExPASy TrEMBL
Match: A0A5A7T7B4 (Putative cyclic nucleotide-gated ion channel 14 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold92G002990 PE=4 SV=1)

HSP 1 Score: 1211.4 bits (3133), Expect = 0.0e+00
Identity = 631/734 (85.97%), Postives = 648/734 (88.28%), Query Frame = 0

Query: 1   MELKKAKFVRFYSNEKKNIELPWEKNDG-KKRNVEFPLPVFKMGKKLENESKIMNSDSSG 60
           ME  K KFVRFYSN         EKNDG KK+ +EFPLP+FKMG KLE+ESK+   DSS 
Sbjct: 1   MEHNKPKFVRFYSN--------LEKNDGNKKKGIEFPLPIFKMGTKLESESKV---DSS- 60

Query: 61  RSKVFVESNGPQEEKNKRILNPESERILRWNRVFLFSCLTALFVDPLFFYLPSVIHHNRS 120
                      QE+KNKRILNPESERILRWNRVFLFSCLTALFVDPLFFYLPSVIHHNRS
Sbjct: 61  -----------QEQKNKRILNPESERILRWNRVFLFSCLTALFVDPLFFYLPSVIHHNRS 120

Query: 121 SCMTTDFNLGIVVTVFRTFADVFYLLHMILKFRIAYVSPTSRVFGK-------------- 180
           SCMTTDFNLGIVVTVFRTFADVFYLLHMILKFRIAYVSPTSRVFGK              
Sbjct: 121 SCMTTDFNLGIVVTVFRTFADVFYLLHMILKFRIAYVSPTSRVFGKGELVTDPKKIAERY 180

Query: 181 -------------------------AIRSSHADHTNNTLVLIVLLQYIPRFYLIFPLSSH 240
                                    AIRSSHADHTNNTLVLIVLLQYIPRFYLIFPLSSH
Sbjct: 181 LKSDFCIDLIASLPLPQIMIWFIMPAIRSSHADHTNNTLVLIVLLQYIPRFYLIFPLSSH 240

Query: 241 IIKTTGVVTKTAWAGAAYNLVLYMLASHILGAAWYLLSVERHAMCWKFACRNEFSPMKCF 300
           IIKTTGVVTKTAWAGAAYNL+LYMLASHILGAAWYLLSVERHAMCWKF CR EFSPMKC 
Sbjct: 241 IIKTTGVVTKTAWAGAAYNLILYMLASHILGAAWYLLSVERHAMCWKFTCRREFSPMKCL 300

Query: 301 LDYLDCGTLNHVDRRIWEVNTTVFSQCSPDEDVIFNYGIFANAIAKDVISSRFLQKYFYC 360
           LDYLDCGTLN VDRRIWEVNTTVFSQCSPDEDV+FNYGIFA+AI K+VISSRFLQKYFYC
Sbjct: 301 LDYLDCGTLNDVDRRIWEVNTTVFSQCSPDEDVVFNYGIFADAITKNVISSRFLQKYFYC 360

Query: 361 LWWGLQNLRYEYICDSYGQGLETSIYMGETLFAILIAIMGLVLFAHLIGNMQTYLQSITV 420
           LWWGLQNL       SYGQ LETSIY+GETLFAILIAIMGLVLFAHLIGNMQTYLQSITV
Sbjct: 361 LWWGLQNL------SSYGQSLETSIYIGETLFAILIAIMGLVLFAHLIGNMQTYLQSITV 420

Query: 421 RLEEWRVKRRDTEEWMKHRQLPRDLQERVRRFVQYKWLATRGVDEESILQGLPTDLRRDI 480
           RLEEWRVKRRDTEEWMKHRQLP+DLQERVRRFVQYKWLATRGVDEESILQGLPTDLRRDI
Sbjct: 421 RLEEWRVKRRDTEEWMKHRQLPQDLQERVRRFVQYKWLATRGVDEESILQGLPTDLRRDI 480

Query: 481 QRHLCLDLVRRVPFFAQMDDQLLDAICERLASSLCTQRTYIVREGDPVTEMLFIIRGMLE 540
           QRHLCLDLVRRVPFFAQMDDQLLDAICERLASSLCTQRTYIVREGDPVTEMLFIIRGMLE
Sbjct: 481 QRHLCLDLVRRVPFFAQMDDQLLDAICERLASSLCTQRTYIVREGDPVTEMLFIIRGMLE 540

Query: 541 SSTTDGGRSGFFNSITLRPGDFCGEELLAWALLPKSSISLPSSTRTVRAITEVEAFALRA 600
           SSTTDGGR GFFNSITLRPGDFCGEELLAWALLPKSSISLPSSTRTVRAITEVEAFALRA
Sbjct: 541 SSTTDGGRLGFFNSITLRPGDFCGEELLAWALLPKSSISLPSSTRTVRAITEVEAFALRA 600

Query: 601 EDLKFVANQFRRLHSKKLQHTFRFYSYHWRTWAACFIQAAWRRFKRRIIAKSLSLQESFS 660
           EDLKFVANQFRRLHSKKLQHTFRFYSYHWRTWAACFIQAAWRRFKRR+IAKSLSLQESFS
Sbjct: 601 EDLKFVANQFRRLHSKKLQHTFRFYSYHWRTWAACFIQAAWRRFKRRVIAKSLSLQESFS 660

Query: 661 MTPEKPVAEETEQEEEEHNTPRSNYSQAKQNLGVTILASRFAANTRRGAQKLKDINLSKL 695
           +TPEKPVA+E EQ EE HNTPRS+YSQAKQNLGVTILASRFAANTRRGAQKLKD+NLSKL
Sbjct: 661 LTPEKPVADEAEQGEEGHNTPRSSYSQAKQNLGVTILASRFAANTRRGAQKLKDVNLSKL 705

BLAST of Lsi04G022030 vs. ExPASy TrEMBL
Match: A0A1S3CQV9 (probable cyclic nucleotide-gated ion channel 14 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103503747 PE=4 SV=1)

HSP 1 Score: 1211.4 bits (3133), Expect = 0.0e+00
Identity = 631/734 (85.97%), Postives = 648/734 (88.28%), Query Frame = 0

Query: 1   MELKKAKFVRFYSNEKKNIELPWEKNDG-KKRNVEFPLPVFKMGKKLENESKIMNSDSSG 60
           ME  K KFVRFYSN         EKNDG KK+ +EFPLP+FKMG KLE+ESK+   DSS 
Sbjct: 1   MEHNKPKFVRFYSN--------LEKNDGNKKKGIEFPLPIFKMGTKLESESKV---DSS- 60

Query: 61  RSKVFVESNGPQEEKNKRILNPESERILRWNRVFLFSCLTALFVDPLFFYLPSVIHHNRS 120
                      QE+KNKRILNPESERILRWNRVFLFSCLTALFVDPLFFYLPSVIHHNRS
Sbjct: 61  -----------QEQKNKRILNPESERILRWNRVFLFSCLTALFVDPLFFYLPSVIHHNRS 120

Query: 121 SCMTTDFNLGIVVTVFRTFADVFYLLHMILKFRIAYVSPTSRVFGK-------------- 180
           SCMTTDFNLGIVVTVFRTFADVFYLLHMILKFRIAYVSPTSRVFGK              
Sbjct: 121 SCMTTDFNLGIVVTVFRTFADVFYLLHMILKFRIAYVSPTSRVFGKGELVTDPKKIAERY 180

Query: 181 -------------------------AIRSSHADHTNNTLVLIVLLQYIPRFYLIFPLSSH 240
                                    AIRSSHADHTNNTLVLIVLLQYIPRFYLIFPLSSH
Sbjct: 181 LKSDFCIDLIASLPLPQIMIWFIMPAIRSSHADHTNNTLVLIVLLQYIPRFYLIFPLSSH 240

Query: 241 IIKTTGVVTKTAWAGAAYNLVLYMLASHILGAAWYLLSVERHAMCWKFACRNEFSPMKCF 300
           IIKTTGVVTKTAWAGAAYNL+LYMLASHILGAAWYLLSVERHAMCWKF CR EFSPMKC 
Sbjct: 241 IIKTTGVVTKTAWAGAAYNLILYMLASHILGAAWYLLSVERHAMCWKFTCRREFSPMKCL 300

Query: 301 LDYLDCGTLNHVDRRIWEVNTTVFSQCSPDEDVIFNYGIFANAIAKDVISSRFLQKYFYC 360
           LDYLDCGTLN VDRRIWEVNTTVFSQCSPDEDV+FNYGIFA+AI K+VISSRFLQKYFYC
Sbjct: 301 LDYLDCGTLNDVDRRIWEVNTTVFSQCSPDEDVVFNYGIFADAITKNVISSRFLQKYFYC 360

Query: 361 LWWGLQNLRYEYICDSYGQGLETSIYMGETLFAILIAIMGLVLFAHLIGNMQTYLQSITV 420
           LWWGLQNL       SYGQ LETSIY+GETLFAILIAIMGLVLFAHLIGNMQTYLQSITV
Sbjct: 361 LWWGLQNL------SSYGQSLETSIYIGETLFAILIAIMGLVLFAHLIGNMQTYLQSITV 420

Query: 421 RLEEWRVKRRDTEEWMKHRQLPRDLQERVRRFVQYKWLATRGVDEESILQGLPTDLRRDI 480
           RLEEWRVKRRDTEEWMKHRQLP+DLQERVRRFVQYKWLATRGVDEESILQGLPTDLRRDI
Sbjct: 421 RLEEWRVKRRDTEEWMKHRQLPQDLQERVRRFVQYKWLATRGVDEESILQGLPTDLRRDI 480

Query: 481 QRHLCLDLVRRVPFFAQMDDQLLDAICERLASSLCTQRTYIVREGDPVTEMLFIIRGMLE 540
           QRHLCLDLVRRVPFFAQMDDQLLDAICERLASSLCTQRTYIVREGDPVTEMLFIIRGMLE
Sbjct: 481 QRHLCLDLVRRVPFFAQMDDQLLDAICERLASSLCTQRTYIVREGDPVTEMLFIIRGMLE 540

Query: 541 SSTTDGGRSGFFNSITLRPGDFCGEELLAWALLPKSSISLPSSTRTVRAITEVEAFALRA 600
           SSTTDGGR GFFNSITLRPGDFCGEELLAWALLPKSSISLPSSTRTVRAITEVEAFALRA
Sbjct: 541 SSTTDGGRLGFFNSITLRPGDFCGEELLAWALLPKSSISLPSSTRTVRAITEVEAFALRA 600

Query: 601 EDLKFVANQFRRLHSKKLQHTFRFYSYHWRTWAACFIQAAWRRFKRRIIAKSLSLQESFS 660
           EDLKFVANQFRRLHSKKLQHTFRFYSYHWRTWAACFIQAAWRRFKRR+IAKSLSLQESFS
Sbjct: 601 EDLKFVANQFRRLHSKKLQHTFRFYSYHWRTWAACFIQAAWRRFKRRVIAKSLSLQESFS 660

Query: 661 MTPEKPVAEETEQEEEEHNTPRSNYSQAKQNLGVTILASRFAANTRRGAQKLKDINLSKL 695
           +TPEKPVA+E EQ EE HNTPRS+YSQAKQNLGVTILASRFAANTRRGAQKLKD+NLSKL
Sbjct: 661 LTPEKPVADEAEQGEEGHNTPRSSYSQAKQNLGVTILASRFAANTRRGAQKLKDVNLSKL 705

BLAST of Lsi04G022030 vs. ExPASy TrEMBL
Match: A0A0A0LGT4 (Cyclic nucleotide-binding domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G881910 PE=4 SV=1)

HSP 1 Score: 1208.0 bits (3124), Expect = 0.0e+00
Identity = 632/734 (86.10%), Postives = 647/734 (88.15%), Query Frame = 0

Query: 1   MELKKAKFVRFYSNEKKNIELPWEKNDG-KKRNVEFPLPVFKMGKKLENESKIMNSDSSG 60
           ME  K KFVRFYSN         EKNDG KK+ +EFPLPVFKMG KLE+ESK+   DSS 
Sbjct: 1   MEHNKRKFVRFYSN--------LEKNDGNKKKGIEFPLPVFKMGTKLESESKV---DSS- 60

Query: 61  RSKVFVESNGPQEEKNKRILNPESERILRWNRVFLFSCLTALFVDPLFFYLPSVIHHNRS 120
                      QE+KNKRILNPESERILRWNRVFLFSCLTALFVDPLFFYLPSVIHHNRS
Sbjct: 61  -----------QEQKNKRILNPESERILRWNRVFLFSCLTALFVDPLFFYLPSVIHHNRS 120

Query: 121 SCMTTDFNLGIVVTVFRTFADVFYLLHMILKFRIAYVSPTSRVFGK-------------- 180
           SCMTTDFNLGIVVTVFRTFADVFYLLHMILKFRIAYVSPTSRVFGK              
Sbjct: 121 SCMTTDFNLGIVVTVFRTFADVFYLLHMILKFRIAYVSPTSRVFGKGELVTDPKKIAERY 180

Query: 181 -------------------------AIRSSHADHTNNTLVLIVLLQYIPRFYLIFPLSSH 240
                                    AIRSSHADHTNNTLVLIVLLQYIPRFYLIFPLSSH
Sbjct: 181 LKSDFCVDLIASLPLPQIMIWFIMPAIRSSHADHTNNTLVLIVLLQYIPRFYLIFPLSSH 240

Query: 241 IIKTTGVVTKTAWAGAAYNLVLYMLASHILGAAWYLLSVERHAMCWKFACRNEFSPMKCF 300
           IIKTTGVVTKTAWAGAAYNLVLYMLASHILGAAWYLLSVERHAMCWKF CR EFSPMKC 
Sbjct: 241 IIKTTGVVTKTAWAGAAYNLVLYMLASHILGAAWYLLSVERHAMCWKFTCRREFSPMKCH 300

Query: 301 LDYLDCGTLNHVDRRIWEVNTTVFSQCSPDEDVIFNYGIFANAIAKDVISSRFLQKYFYC 360
           LDYLDCGTLN VDRRIWEVNTTVFSQCSPDED +FNYGIFA+AI K+VISSRFLQKYFYC
Sbjct: 301 LDYLDCGTLNDVDRRIWEVNTTVFSQCSPDEDFVFNYGIFADAITKNVISSRFLQKYFYC 360

Query: 361 LWWGLQNLRYEYICDSYGQGLETSIYMGETLFAILIAIMGLVLFAHLIGNMQTYLQSITV 420
           LWWGLQNL       SYGQ LETSI++GETLFAILIAIMGLVLFAHLIGNMQTYLQSITV
Sbjct: 361 LWWGLQNL------SSYGQSLETSIFIGETLFAILIAIMGLVLFAHLIGNMQTYLQSITV 420

Query: 421 RLEEWRVKRRDTEEWMKHRQLPRDLQERVRRFVQYKWLATRGVDEESILQGLPTDLRRDI 480
           RLEEWRVKRRDTEEWMKHRQLP+DLQERVRRFVQYKWLATRGVDEESILQGLPTDLRRDI
Sbjct: 421 RLEEWRVKRRDTEEWMKHRQLPQDLQERVRRFVQYKWLATRGVDEESILQGLPTDLRRDI 480

Query: 481 QRHLCLDLVRRVPFFAQMDDQLLDAICERLASSLCTQRTYIVREGDPVTEMLFIIRGMLE 540
           QRHLCLDLVRRVPFFAQMD QLLDAICERLASSLCTQRTYIVREGDPVTEMLFIIRGMLE
Sbjct: 481 QRHLCLDLVRRVPFFAQMDGQLLDAICERLASSLCTQRTYIVREGDPVTEMLFIIRGMLE 540

Query: 541 SSTTDGGRSGFFNSITLRPGDFCGEELLAWALLPKSSISLPSSTRTVRAITEVEAFALRA 600
           SSTTDGGR GFFNSITLRPGDFCGEELLAWALLPKSSISLPSSTRTVRAITEVEAFALRA
Sbjct: 541 SSTTDGGRLGFFNSITLRPGDFCGEELLAWALLPKSSISLPSSTRTVRAITEVEAFALRA 600

Query: 601 EDLKFVANQFRRLHSKKLQHTFRFYSYHWRTWAACFIQAAWRRFKRRIIAKSLSLQESFS 660
           EDLKFVANQFRRLHSKKLQHTFRFYSYHWRTWAACFIQAAWRRFKRRIIAKSLSLQESFS
Sbjct: 601 EDLKFVANQFRRLHSKKLQHTFRFYSYHWRTWAACFIQAAWRRFKRRIIAKSLSLQESFS 660

Query: 661 MTPEKPVAEETEQEEEEHNTPRSNYSQAKQNLGVTILASRFAANTRRGAQKLKDINLSKL 695
           +TPEKPVAEE EQEEE H+TPRS+YSQAKQNLGVTILASRFAANTRRGAQKLKD+NLSKL
Sbjct: 661 LTPEKPVAEEAEQEEEGHSTPRSSYSQAKQNLGVTILASRFAANTRRGAQKLKDVNLSKL 705

BLAST of Lsi04G022030 vs. ExPASy TrEMBL
Match: A0A6J1J1M1 (probable cyclic nucleotide-gated ion channel 14 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111481036 PE=4 SV=1)

HSP 1 Score: 1191.0 bits (3080), Expect = 0.0e+00
Identity = 619/738 (83.88%), Postives = 645/738 (87.40%), Query Frame = 0

Query: 1   MELKKAKFVRFYSNEKKNIELPWEKNDGKKRNVEFPLPVFKMG-----KKLENESKIMNS 60
           MELKK KFVRFYSNEKKN+ELPWEKNDGK+R  EF LPVFKMG       + ++ KI  S
Sbjct: 10  MELKKGKFVRFYSNEKKNLELPWEKNDGKRR-FEFALPVFKMGTAPLTNAVGDKGKI--S 69

Query: 61  DSSGRSKVFVESNGPQEEKNKRILNPESERILRWNRVFLFSCLTALFVDPLFFYLPSVIH 120
              GR K+FVE+N   E + K ILNPESE ILRWNRVFLFSCLTALFVDPLFFYLPSVIH
Sbjct: 70  SDLGRFKIFVENN---EAQQKWILNPESETILRWNRVFLFSCLTALFVDPLFFYLPSVIH 129

Query: 121 HNRSSCMTTDFNLGIVVTVFRTFADVFYLLHMILKFRIAYVSPTSRVFGK---------- 180
           HNRSSCMTTDFNLGI VTVFRTFADVFYL HM+LKFR+AYVSPTSRVFGK          
Sbjct: 130 HNRSSCMTTDFNLGIAVTVFRTFADVFYLFHMVLKFRMAYVSPTSRVFGKGELVTDPKKI 189

Query: 181 -----------------------------AIRSSHADHTNNTLVLIVLLQYIPRFYLIFP 240
                                        AIRSSHADHT+NTLVLIVLLQYIPRFYLIFP
Sbjct: 190 AKRYLKSDFCIDLIASLPLPQIMIWFIMPAIRSSHADHTSNTLVLIVLLQYIPRFYLIFP 249

Query: 241 LSSHIIKTTGVVTKTAWAGAAYNLVLYMLASHILGAAWYLLSVERHAMCWKFACRNEFSP 300
           LSSHIIKTTGVVTKTAWAGAAYNLVLYMLASHILGAAWYLLSVERHAMCWKF CR EFSP
Sbjct: 250 LSSHIIKTTGVVTKTAWAGAAYNLVLYMLASHILGAAWYLLSVERHAMCWKFTCRREFSP 309

Query: 301 MKCFLDYLDCGTLNHVDRRIWEVNTTVFSQCSPDEDVIFNYGIFANAIAKDVISSRFLQK 360
           MKC LDYLDCGTLN VDRRIWEVNTTVF+QCSPDEDV+FNYGIFANAI+K+VIS  F+QK
Sbjct: 310 MKCHLDYLDCGTLNDVDRRIWEVNTTVFAQCSPDEDVVFNYGIFANAISKNVISFGFIQK 369

Query: 361 YFYCLWWGLQNLRYEYICDSYGQGLETSIYMGETLFAILIAIMGLVLFAHLIGNMQTYLQ 420
           YFYCLWWGLQNL       SYGQGLETS ++GET FAILIAIMGLVLFAHLIGNMQTYLQ
Sbjct: 370 YFYCLWWGLQNL------SSYGQGLETSTFIGETSFAILIAIMGLVLFAHLIGNMQTYLQ 429

Query: 421 SITVRLEEWRVKRRDTEEWMKHRQLPRDLQERVRRFVQYKWLATRGVDEESILQGLPTDL 480
           SITVRLEEWRVKRRDTEEWM HRQLP DLQERVRRFVQYKWLATRGVDEESILQ LPTDL
Sbjct: 430 SITVRLEEWRVKRRDTEEWMNHRQLPEDLQERVRRFVQYKWLATRGVDEESILQALPTDL 489

Query: 481 RRDIQRHLCLDLVRRVPFFAQMDDQLLDAICERLASSLCTQRTYIVREGDPVTEMLFIIR 540
           RRDIQRHLCLDLVRRVPFFAQMDDQLLDAICERLASSLCTQ T+IVREGDPVTEM FIIR
Sbjct: 490 RRDIQRHLCLDLVRRVPFFAQMDDQLLDAICERLASSLCTQGTFIVREGDPVTEMFFIIR 549

Query: 541 GMLESSTTDGGRSGFFNSITLRPGDFCGEELLAWALLPKSSISLPSSTRTVRAITEVEAF 600
           GMLESSTTDGGRSGFFNSITLRPGDFCGEELLAWALLPKSSISLPSSTRTVRAITEVEAF
Sbjct: 550 GMLESSTTDGGRSGFFNSITLRPGDFCGEELLAWALLPKSSISLPSSTRTVRAITEVEAF 609

Query: 601 ALRAEDLKFVANQFRRLHSKKLQHTFRFYSYHWRTWAACFIQAAWRRFKRRIIAKSLSLQ 660
           ALRAEDLKFVANQFRRLHSKKLQHTFRFYSYHWRTWAACFIQAAWRRFKRRIIAK+LS+Q
Sbjct: 610 ALRAEDLKFVANQFRRLHSKKLQHTFRFYSYHWRTWAACFIQAAWRRFKRRIIAKTLSIQ 669

Query: 661 ESFSMTPEKPVAEETEQEEEEHNTPRSNYSQAKQNLGVTILASRFAANTRRGAQKLKDIN 695
           ESFS+ P+KP+A+E E EEEEHN PRSNYS AKQNLGVTILASRFAANTRRGAQKLKD+N
Sbjct: 670 ESFSLNPKKPMADEAELEEEEHN-PRSNYSHAKQNLGVTILASRFAANTRRGAQKLKDVN 729

BLAST of Lsi04G022030 vs. ExPASy TrEMBL
Match: A0A6J1CLB2 (probable cyclic nucleotide-gated ion channel 14 OS=Momordica charantia OX=3673 GN=LOC111012218 PE=4 SV=1)

HSP 1 Score: 1188.7 bits (3074), Expect = 0.0e+00
Identity = 617/742 (83.15%), Postives = 653/742 (88.01%), Query Frame = 0

Query: 1   MELKKAKFVRFYSNEKKNIELPWEKNDGKKRNVEFPLPVFKMG----KKLE----NESKI 60
           MELKK KFVRFYS+E +N+ELPWEKND K+R +  PLPVFK+G     KL+    +++KI
Sbjct: 1   MELKKGKFVRFYSDENQNLELPWEKNDPKRR-IHLPLPVFKVGTAPLAKLDAGVGDKTKI 60

Query: 61  -MNSDSSGRSKVFVESNGPQEEKNKRILNPESERILRWNRVFLFSCLTALFVDPLFFYLP 120
              +   GRSK+FVES+GP     KRILNPESE ILRWNRVFLFSCLTALFVDPLFFYLP
Sbjct: 61  NYGNPDLGRSKIFVESSGP---FFKRILNPESETILRWNRVFLFSCLTALFVDPLFFYLP 120

Query: 121 SVIHHNRSSCMTTDFNLGIVVTVFRTFADVFYLLHMILKFRIAYVSPTSRVFGK------ 180
           SVIHH+RSSCMTTDFNLGIVVTVFRTFADVFYL HM+LKFR+AYVSP SRVFGK      
Sbjct: 121 SVIHHHRSSCMTTDFNLGIVVTVFRTFADVFYLFHMVLKFRMAYVSPISRVFGKGELVTD 180

Query: 181 ---------------------------------AIRSSHADHTNNTLVLIVLLQYIPRFY 240
                                            AIRSSHADHTNNTLVLIVLLQYIPRFY
Sbjct: 181 PKMIAKRYLKSDFFIDLIASLPLPQIMIWFIMPAIRSSHADHTNNTLVLIVLLQYIPRFY 240

Query: 241 LIFPLSSHIIKTTGVVTKTAWAGAAYNLVLYMLASHILGAAWYLLSVERHAMCWKFACRN 300
           LIFPLSSHIIKTTGVVTKTAWAGAAYNLVLYMLASHILGAAWYLLSVERHAMCWK  CR 
Sbjct: 241 LIFPLSSHIIKTTGVVTKTAWAGAAYNLVLYMLASHILGAAWYLLSVERHAMCWKLTCRR 300

Query: 301 EFSPMKCFLDYLDCGTLNHVDRRIWEVNTTVFSQCSPDEDVIFNYGIFANAIAKDVISSR 360
           E SP+KC LDYLDCGTL++VDRRIWE+NTTVFSQCSPDE+ +FNYGIFA+AI+K+VISS+
Sbjct: 301 ELSPVKCLLDYLDCGTLDYVDRRIWELNTTVFSQCSPDEENVFNYGIFADAISKNVISSK 360

Query: 361 FLQKYFYCLWWGLQNLRYEYICDSYGQGLETSIYMGETLFAILIAIMGLVLFAHLIGNMQ 420
           FLQKYFYCLWWGLQNL       SYGQGL+T+ ++GETLFAILIAIMGLVLFAHLIGNMQ
Sbjct: 361 FLQKYFYCLWWGLQNL------SSYGQGLDTTTFIGETLFAILIAIMGLVLFAHLIGNMQ 420

Query: 421 TYLQSITVRLEEWRVKRRDTEEWMKHRQLPRDLQERVRRFVQYKWLATRGVDEESILQGL 480
           TYLQSITVRLEE RVKRRDTEEWMKHRQLP+DLQERVRRFVQYKWLATRGVDEESILQGL
Sbjct: 421 TYLQSITVRLEECRVKRRDTEEWMKHRQLPQDLQERVRRFVQYKWLATRGVDEESILQGL 480

Query: 481 PTDLRRDIQRHLCLDLVRRVPFFAQMDDQLLDAICERLASSLCTQRTYIVREGDPVTEML 540
           PTDLRRDIQRHLCLDLVRRVPFFAQMDDQLLDAICERLASSLCTQ TYIVREGDPVTEML
Sbjct: 481 PTDLRRDIQRHLCLDLVRRVPFFAQMDDQLLDAICERLASSLCTQGTYIVREGDPVTEML 540

Query: 541 FIIRGMLESSTTDGGRSGFFNSITLRPGDFCGEELLAWALLPKSSISLPSSTRTVRAITE 600
           FIIRGMLESSTTDGGRSGFFNSITLRPGDFCGEELLAWALLPKSSISLPSSTRTVRAITE
Sbjct: 541 FIIRGMLESSTTDGGRSGFFNSITLRPGDFCGEELLAWALLPKSSISLPSSTRTVRAITE 600

Query: 601 VEAFALRAEDLKFVANQFRRLHSKKLQHTFRFYSYHWRTWAACFIQAAWRRFKRRIIAKS 660
           VEAFALRAEDLKFVANQFRRLHSKKLQHTFRFYSYHWRTWAACFIQAAWRRFKRR+IAKS
Sbjct: 601 VEAFALRAEDLKFVANQFRRLHSKKLQHTFRFYSYHWRTWAACFIQAAWRRFKRRVIAKS 660

Query: 661 LSLQESFSMTPEKPVAEETEQEEEEHNTPRSNYSQAKQNLGVTILASRFAANTRRGAQKL 695
           LS+QESFS+TP+KP AEE EQEEEEHN PRS YSQAKQNLGVTILASRFAANTRRGAQKL
Sbjct: 661 LSMQESFSLTPKKPAAEEAEQEEEEHNIPRSKYSQAKQNLGVTILASRFAANTRRGAQKL 720

BLAST of Lsi04G022030 vs. NCBI nr
Match: XP_038896574.1 (probable cyclic nucleotide-gated ion channel 14 isoform X1 [Benincasa hispida])

HSP 1 Score: 1273.5 bits (3294), Expect = 0.0e+00
Identity = 654/733 (89.22%), Postives = 669/733 (91.27%), Query Frame = 0

Query: 1   MELKKAKFVRFYSNEKKNIELPWEKNDGKKRNVEFPLPVFKMGKKLENESKIMNSDSSGR 60
           MELKK KFVRF+SN         EKNDG KR VEFPLPVF+MGKKLENESK+MNSDSSGR
Sbjct: 10  MELKKGKFVRFHSN--------LEKNDGNKRRVEFPLPVFRMGKKLENESKVMNSDSSGR 69

Query: 61  SKVFVESNGPQEEKNKRILNPESERILRWNRVFLFSCLTALFVDPLFFYLPSVIHHNRSS 120
           SKVFVE+NG QE+KNKRILNPESERILRWNRVFLFSCLTALFVDPLFFYLPSVIHHNRSS
Sbjct: 70  SKVFVETNGLQEQKNKRILNPESERILRWNRVFLFSCLTALFVDPLFFYLPSVIHHNRSS 129

Query: 121 CMTTDFNLGIVVTVFRTFADVFYLLHMILKFRIAYVSPTSRVFGK--------------- 180
           CMTTDFNLGIVVTVFRTFADVFYLLHMILKFRIAYVSPTSRVFGK               
Sbjct: 130 CMTTDFNLGIVVTVFRTFADVFYLLHMILKFRIAYVSPTSRVFGKGELVTDPKKIAKRYL 189

Query: 181 ------------------------AIRSSHADHTNNTLVLIVLLQYIPRFYLIFPLSSHI 240
                                   AIRSSHADHTNNTLVLIVLLQYIPRFYLIFPLSSHI
Sbjct: 190 KSDFCIDLIASLPLPQIMIWFIMPAIRSSHADHTNNTLVLIVLLQYIPRFYLIFPLSSHI 249

Query: 241 IKTTGVVTKTAWAGAAYNLVLYMLASHILGAAWYLLSVERHAMCWKFACRNEFSPMKCFL 300
           IKTTGVVTKTAWAGAAYNL+LYMLASHILGAAWYLLSVERHAMCWKF CR EFSPMKC L
Sbjct: 250 IKTTGVVTKTAWAGAAYNLILYMLASHILGAAWYLLSVERHAMCWKFTCRREFSPMKCLL 309

Query: 301 DYLDCGTLNHVDRRIWEVNTTVFSQCSPDEDVIFNYGIFANAIAKDVISSRFLQKYFYCL 360
           DYLDCGTLN VDRRIWEVNTTVFSQCSPDEDV+FNYGIFA+AIAKDVISSRFLQKYFYCL
Sbjct: 310 DYLDCGTLNDVDRRIWEVNTTVFSQCSPDEDVVFNYGIFADAIAKDVISSRFLQKYFYCL 369

Query: 361 WWGLQNLRYEYICDSYGQGLETSIYMGETLFAILIAIMGLVLFAHLIGNMQTYLQSITVR 420
           WWGLQNL       SYGQGLETSI++GETLFAILIAIMGLVLFAHLIGNMQTYLQSITVR
Sbjct: 370 WWGLQNL------SSYGQGLETSIFIGETLFAILIAIMGLVLFAHLIGNMQTYLQSITVR 429

Query: 421 LEEWRVKRRDTEEWMKHRQLPRDLQERVRRFVQYKWLATRGVDEESILQGLPTDLRRDIQ 480
           LEEWRVKRRDTEEWMKHRQLP+DLQERVRRFVQYKWLATRGVDEESILQGLPTDLRRDIQ
Sbjct: 430 LEEWRVKRRDTEEWMKHRQLPQDLQERVRRFVQYKWLATRGVDEESILQGLPTDLRRDIQ 489

Query: 481 RHLCLDLVRRVPFFAQMDDQLLDAICERLASSLCTQRTYIVREGDPVTEMLFIIRGMLES 540
           RHLCLDLVRRVPFFAQMDDQLLDAICERLASSLCTQRTYIVREGDPVTEMLFIIRGMLES
Sbjct: 490 RHLCLDLVRRVPFFAQMDDQLLDAICERLASSLCTQRTYIVREGDPVTEMLFIIRGMLES 549

Query: 541 STTDGGRSGFFNSITLRPGDFCGEELLAWALLPKSSISLPSSTRTVRAITEVEAFALRAE 600
           STTDGGRSGFFNSITLRPGDFCGEELLAWALLPKSSISLPSSTRTVRAITEVEAFALRAE
Sbjct: 550 STTDGGRSGFFNSITLRPGDFCGEELLAWALLPKSSISLPSSTRTVRAITEVEAFALRAE 609

Query: 601 DLKFVANQFRRLHSKKLQHTFRFYSYHWRTWAACFIQAAWRRFKRRIIAKSLSLQESFSM 660
           DLKFVANQFRRLHSKKLQHTFRFYSYHWRTWAACFIQAAWRRFKRRIIAKSLSLQESFS+
Sbjct: 610 DLKFVANQFRRLHSKKLQHTFRFYSYHWRTWAACFIQAAWRRFKRRIIAKSLSLQESFSL 669

Query: 661 TPEKPVAEETEQEEEEHNTPRSNYSQAKQNLGVTILASRFAANTRRGAQKLKDINLSKLR 695
           TPEKPVA+E EQEEEEHNTPR+NYSQAKQNLGVTILASRFAANTRRGAQKLKD+NLSKLR
Sbjct: 670 TPEKPVADEAEQEEEEHNTPRTNYSQAKQNLGVTILASRFAANTRRGAQKLKDVNLSKLR 728

BLAST of Lsi04G022030 vs. NCBI nr
Match: XP_008466292.1 (PREDICTED: probable cyclic nucleotide-gated ion channel 14 isoform X1 [Cucumis melo] >KAA0038748.1 putative cyclic nucleotide-gated ion channel 14 isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 1211.4 bits (3133), Expect = 0.0e+00
Identity = 631/734 (85.97%), Postives = 648/734 (88.28%), Query Frame = 0

Query: 1   MELKKAKFVRFYSNEKKNIELPWEKNDG-KKRNVEFPLPVFKMGKKLENESKIMNSDSSG 60
           ME  K KFVRFYSN         EKNDG KK+ +EFPLP+FKMG KLE+ESK+   DSS 
Sbjct: 1   MEHNKPKFVRFYSN--------LEKNDGNKKKGIEFPLPIFKMGTKLESESKV---DSS- 60

Query: 61  RSKVFVESNGPQEEKNKRILNPESERILRWNRVFLFSCLTALFVDPLFFYLPSVIHHNRS 120
                      QE+KNKRILNPESERILRWNRVFLFSCLTALFVDPLFFYLPSVIHHNRS
Sbjct: 61  -----------QEQKNKRILNPESERILRWNRVFLFSCLTALFVDPLFFYLPSVIHHNRS 120

Query: 121 SCMTTDFNLGIVVTVFRTFADVFYLLHMILKFRIAYVSPTSRVFGK-------------- 180
           SCMTTDFNLGIVVTVFRTFADVFYLLHMILKFRIAYVSPTSRVFGK              
Sbjct: 121 SCMTTDFNLGIVVTVFRTFADVFYLLHMILKFRIAYVSPTSRVFGKGELVTDPKKIAERY 180

Query: 181 -------------------------AIRSSHADHTNNTLVLIVLLQYIPRFYLIFPLSSH 240
                                    AIRSSHADHTNNTLVLIVLLQYIPRFYLIFPLSSH
Sbjct: 181 LKSDFCIDLIASLPLPQIMIWFIMPAIRSSHADHTNNTLVLIVLLQYIPRFYLIFPLSSH 240

Query: 241 IIKTTGVVTKTAWAGAAYNLVLYMLASHILGAAWYLLSVERHAMCWKFACRNEFSPMKCF 300
           IIKTTGVVTKTAWAGAAYNL+LYMLASHILGAAWYLLSVERHAMCWKF CR EFSPMKC 
Sbjct: 241 IIKTTGVVTKTAWAGAAYNLILYMLASHILGAAWYLLSVERHAMCWKFTCRREFSPMKCL 300

Query: 301 LDYLDCGTLNHVDRRIWEVNTTVFSQCSPDEDVIFNYGIFANAIAKDVISSRFLQKYFYC 360
           LDYLDCGTLN VDRRIWEVNTTVFSQCSPDEDV+FNYGIFA+AI K+VISSRFLQKYFYC
Sbjct: 301 LDYLDCGTLNDVDRRIWEVNTTVFSQCSPDEDVVFNYGIFADAITKNVISSRFLQKYFYC 360

Query: 361 LWWGLQNLRYEYICDSYGQGLETSIYMGETLFAILIAIMGLVLFAHLIGNMQTYLQSITV 420
           LWWGLQNL       SYGQ LETSIY+GETLFAILIAIMGLVLFAHLIGNMQTYLQSITV
Sbjct: 361 LWWGLQNL------SSYGQSLETSIYIGETLFAILIAIMGLVLFAHLIGNMQTYLQSITV 420

Query: 421 RLEEWRVKRRDTEEWMKHRQLPRDLQERVRRFVQYKWLATRGVDEESILQGLPTDLRRDI 480
           RLEEWRVKRRDTEEWMKHRQLP+DLQERVRRFVQYKWLATRGVDEESILQGLPTDLRRDI
Sbjct: 421 RLEEWRVKRRDTEEWMKHRQLPQDLQERVRRFVQYKWLATRGVDEESILQGLPTDLRRDI 480

Query: 481 QRHLCLDLVRRVPFFAQMDDQLLDAICERLASSLCTQRTYIVREGDPVTEMLFIIRGMLE 540
           QRHLCLDLVRRVPFFAQMDDQLLDAICERLASSLCTQRTYIVREGDPVTEMLFIIRGMLE
Sbjct: 481 QRHLCLDLVRRVPFFAQMDDQLLDAICERLASSLCTQRTYIVREGDPVTEMLFIIRGMLE 540

Query: 541 SSTTDGGRSGFFNSITLRPGDFCGEELLAWALLPKSSISLPSSTRTVRAITEVEAFALRA 600
           SSTTDGGR GFFNSITLRPGDFCGEELLAWALLPKSSISLPSSTRTVRAITEVEAFALRA
Sbjct: 541 SSTTDGGRLGFFNSITLRPGDFCGEELLAWALLPKSSISLPSSTRTVRAITEVEAFALRA 600

Query: 601 EDLKFVANQFRRLHSKKLQHTFRFYSYHWRTWAACFIQAAWRRFKRRIIAKSLSLQESFS 660
           EDLKFVANQFRRLHSKKLQHTFRFYSYHWRTWAACFIQAAWRRFKRR+IAKSLSLQESFS
Sbjct: 601 EDLKFVANQFRRLHSKKLQHTFRFYSYHWRTWAACFIQAAWRRFKRRVIAKSLSLQESFS 660

Query: 661 MTPEKPVAEETEQEEEEHNTPRSNYSQAKQNLGVTILASRFAANTRRGAQKLKDINLSKL 695
           +TPEKPVA+E EQ EE HNTPRS+YSQAKQNLGVTILASRFAANTRRGAQKLKD+NLSKL
Sbjct: 661 LTPEKPVADEAEQGEEGHNTPRSSYSQAKQNLGVTILASRFAANTRRGAQKLKDVNLSKL 705

BLAST of Lsi04G022030 vs. NCBI nr
Match: XP_004136445.1 (probable cyclic nucleotide-gated ion channel 14 isoform X1 [Cucumis sativus] >KGN60179.1 hypothetical protein Csa_002518 [Cucumis sativus])

HSP 1 Score: 1208.0 bits (3124), Expect = 0.0e+00
Identity = 632/734 (86.10%), Postives = 647/734 (88.15%), Query Frame = 0

Query: 1   MELKKAKFVRFYSNEKKNIELPWEKNDG-KKRNVEFPLPVFKMGKKLENESKIMNSDSSG 60
           ME  K KFVRFYSN         EKNDG KK+ +EFPLPVFKMG KLE+ESK+   DSS 
Sbjct: 1   MEHNKRKFVRFYSN--------LEKNDGNKKKGIEFPLPVFKMGTKLESESKV---DSS- 60

Query: 61  RSKVFVESNGPQEEKNKRILNPESERILRWNRVFLFSCLTALFVDPLFFYLPSVIHHNRS 120
                      QE+KNKRILNPESERILRWNRVFLFSCLTALFVDPLFFYLPSVIHHNRS
Sbjct: 61  -----------QEQKNKRILNPESERILRWNRVFLFSCLTALFVDPLFFYLPSVIHHNRS 120

Query: 121 SCMTTDFNLGIVVTVFRTFADVFYLLHMILKFRIAYVSPTSRVFGK-------------- 180
           SCMTTDFNLGIVVTVFRTFADVFYLLHMILKFRIAYVSPTSRVFGK              
Sbjct: 121 SCMTTDFNLGIVVTVFRTFADVFYLLHMILKFRIAYVSPTSRVFGKGELVTDPKKIAERY 180

Query: 181 -------------------------AIRSSHADHTNNTLVLIVLLQYIPRFYLIFPLSSH 240
                                    AIRSSHADHTNNTLVLIVLLQYIPRFYLIFPLSSH
Sbjct: 181 LKSDFCVDLIASLPLPQIMIWFIMPAIRSSHADHTNNTLVLIVLLQYIPRFYLIFPLSSH 240

Query: 241 IIKTTGVVTKTAWAGAAYNLVLYMLASHILGAAWYLLSVERHAMCWKFACRNEFSPMKCF 300
           IIKTTGVVTKTAWAGAAYNLVLYMLASHILGAAWYLLSVERHAMCWKF CR EFSPMKC 
Sbjct: 241 IIKTTGVVTKTAWAGAAYNLVLYMLASHILGAAWYLLSVERHAMCWKFTCRREFSPMKCH 300

Query: 301 LDYLDCGTLNHVDRRIWEVNTTVFSQCSPDEDVIFNYGIFANAIAKDVISSRFLQKYFYC 360
           LDYLDCGTLN VDRRIWEVNTTVFSQCSPDED +FNYGIFA+AI K+VISSRFLQKYFYC
Sbjct: 301 LDYLDCGTLNDVDRRIWEVNTTVFSQCSPDEDFVFNYGIFADAITKNVISSRFLQKYFYC 360

Query: 361 LWWGLQNLRYEYICDSYGQGLETSIYMGETLFAILIAIMGLVLFAHLIGNMQTYLQSITV 420
           LWWGLQNL       SYGQ LETSI++GETLFAILIAIMGLVLFAHLIGNMQTYLQSITV
Sbjct: 361 LWWGLQNL------SSYGQSLETSIFIGETLFAILIAIMGLVLFAHLIGNMQTYLQSITV 420

Query: 421 RLEEWRVKRRDTEEWMKHRQLPRDLQERVRRFVQYKWLATRGVDEESILQGLPTDLRRDI 480
           RLEEWRVKRRDTEEWMKHRQLP+DLQERVRRFVQYKWLATRGVDEESILQGLPTDLRRDI
Sbjct: 421 RLEEWRVKRRDTEEWMKHRQLPQDLQERVRRFVQYKWLATRGVDEESILQGLPTDLRRDI 480

Query: 481 QRHLCLDLVRRVPFFAQMDDQLLDAICERLASSLCTQRTYIVREGDPVTEMLFIIRGMLE 540
           QRHLCLDLVRRVPFFAQMD QLLDAICERLASSLCTQRTYIVREGDPVTEMLFIIRGMLE
Sbjct: 481 QRHLCLDLVRRVPFFAQMDGQLLDAICERLASSLCTQRTYIVREGDPVTEMLFIIRGMLE 540

Query: 541 SSTTDGGRSGFFNSITLRPGDFCGEELLAWALLPKSSISLPSSTRTVRAITEVEAFALRA 600
           SSTTDGGR GFFNSITLRPGDFCGEELLAWALLPKSSISLPSSTRTVRAITEVEAFALRA
Sbjct: 541 SSTTDGGRLGFFNSITLRPGDFCGEELLAWALLPKSSISLPSSTRTVRAITEVEAFALRA 600

Query: 601 EDLKFVANQFRRLHSKKLQHTFRFYSYHWRTWAACFIQAAWRRFKRRIIAKSLSLQESFS 660
           EDLKFVANQFRRLHSKKLQHTFRFYSYHWRTWAACFIQAAWRRFKRRIIAKSLSLQESFS
Sbjct: 601 EDLKFVANQFRRLHSKKLQHTFRFYSYHWRTWAACFIQAAWRRFKRRIIAKSLSLQESFS 660

Query: 661 MTPEKPVAEETEQEEEEHNTPRSNYSQAKQNLGVTILASRFAANTRRGAQKLKDINLSKL 695
           +TPEKPVAEE EQEEE H+TPRS+YSQAKQNLGVTILASRFAANTRRGAQKLKD+NLSKL
Sbjct: 661 LTPEKPVAEEAEQEEEGHSTPRSSYSQAKQNLGVTILASRFAANTRRGAQKLKDVNLSKL 705

BLAST of Lsi04G022030 vs. NCBI nr
Match: XP_023524088.1 (probable cyclic nucleotide-gated ion channel 14 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1193.7 bits (3087), Expect = 0.0e+00
Identity = 621/738 (84.15%), Postives = 645/738 (87.40%), Query Frame = 0

Query: 1   MELKKAKFVRFYSNEKKNIELPWEKNDGKKRNVEFPLPVFKMG-----KKLENESKIMNS 60
           MELKK KFVRFYSNEKKN+ELPWEKNDGK+R  EF LPVFKMG       + ++ KI  S
Sbjct: 1   MELKKGKFVRFYSNEKKNLELPWEKNDGKRR-FEFALPVFKMGTAPLTNAVGDKGKI--S 60

Query: 61  DSSGRSKVFVESNGPQEEKNKRILNPESERILRWNRVFLFSCLTALFVDPLFFYLPSVIH 120
              GR K+FVE+N   E + K ILNPESE ILRWNRVFLFSCLTALFVDPLFFYLPSVIH
Sbjct: 61  SDLGRFKIFVENN---EAQQKWILNPESETILRWNRVFLFSCLTALFVDPLFFYLPSVIH 120

Query: 121 HNRSSCMTTDFNLGIVVTVFRTFADVFYLLHMILKFRIAYVSPTSRVFGK---------- 180
           HNRSSCMTTDFNLGI VTVFRTFADVFYL HM+LKFR+AYVSPTSRVFGK          
Sbjct: 121 HNRSSCMTTDFNLGIAVTVFRTFADVFYLFHMVLKFRMAYVSPTSRVFGKGELVTDPKKI 180

Query: 181 -----------------------------AIRSSHADHTNNTLVLIVLLQYIPRFYLIFP 240
                                        AIRSSHADHTNNTLVLIVLLQYIPRFYLIFP
Sbjct: 181 AKRYLKSDFCIDLIASLPLPQIMIWFIMPAIRSSHADHTNNTLVLIVLLQYIPRFYLIFP 240

Query: 241 LSSHIIKTTGVVTKTAWAGAAYNLVLYMLASHILGAAWYLLSVERHAMCWKFACRNEFSP 300
           LSSHIIKTTGVVTKTAWAGAAYNLVLYMLASHILGAAWYLLSVERHAMCWKF CR EFSP
Sbjct: 241 LSSHIIKTTGVVTKTAWAGAAYNLVLYMLASHILGAAWYLLSVERHAMCWKFTCRREFSP 300

Query: 301 MKCFLDYLDCGTLNHVDRRIWEVNTTVFSQCSPDEDVIFNYGIFANAIAKDVISSRFLQK 360
           MKC LDYLDCGTLN VDRRIWEVNTTVF+QCSPDEDV+FNYGIFANAI+K+VIS  F QK
Sbjct: 301 MKCHLDYLDCGTLNDVDRRIWEVNTTVFTQCSPDEDVVFNYGIFANAISKNVISFGFAQK 360

Query: 361 YFYCLWWGLQNLRYEYICDSYGQGLETSIYMGETLFAILIAIMGLVLFAHLIGNMQTYLQ 420
           YFYCLWWGLQNL       SYGQGLETS ++GET FAILIAIMGLVLFAHLIGNMQTYLQ
Sbjct: 361 YFYCLWWGLQNL------SSYGQGLETSTFIGETSFAILIAIMGLVLFAHLIGNMQTYLQ 420

Query: 421 SITVRLEEWRVKRRDTEEWMKHRQLPRDLQERVRRFVQYKWLATRGVDEESILQGLPTDL 480
           SITVRLEEWRVKRRDTEEWM HRQLP DLQERVRRFVQYKWLATRGVDEESILQ LPTDL
Sbjct: 421 SITVRLEEWRVKRRDTEEWMNHRQLPEDLQERVRRFVQYKWLATRGVDEESILQALPTDL 480

Query: 481 RRDIQRHLCLDLVRRVPFFAQMDDQLLDAICERLASSLCTQRTYIVREGDPVTEMLFIIR 540
           RRDIQRHLCLDLVRRVPFFAQMDDQLLDAICERLASSLCTQ T+IVREGDPVTEM FIIR
Sbjct: 481 RRDIQRHLCLDLVRRVPFFAQMDDQLLDAICERLASSLCTQGTFIVREGDPVTEMFFIIR 540

Query: 541 GMLESSTTDGGRSGFFNSITLRPGDFCGEELLAWALLPKSSISLPSSTRTVRAITEVEAF 600
           GMLESSTTDGGRSGFFNSITLRPGDFCGEELLAWALLPKSSISLPSSTRTVRAITEVEAF
Sbjct: 541 GMLESSTTDGGRSGFFNSITLRPGDFCGEELLAWALLPKSSISLPSSTRTVRAITEVEAF 600

Query: 601 ALRAEDLKFVANQFRRLHSKKLQHTFRFYSYHWRTWAACFIQAAWRRFKRRIIAKSLSLQ 660
           ALRAEDLKFVANQFRRLHSKKLQHTFRFYSYHWRTWAACFIQAAWRRFKRRIIAK+LS+Q
Sbjct: 601 ALRAEDLKFVANQFRRLHSKKLQHTFRFYSYHWRTWAACFIQAAWRRFKRRIIAKTLSIQ 660

Query: 661 ESFSMTPEKPVAEETEQEEEEHNTPRSNYSQAKQNLGVTILASRFAANTRRGAQKLKDIN 695
           ESFS+ P+KP+A+E E EEEEHN PRSNYSQAKQNLGVTILASRFAANTRRGAQKLKD+N
Sbjct: 661 ESFSLNPKKPMADEAELEEEEHN-PRSNYSQAKQNLGVTILASRFAANTRRGAQKLKDVN 720

BLAST of Lsi04G022030 vs. NCBI nr
Match: KAG6608211.1 (putative cyclic nucleotide-gated ion channel 14, partial [Cucurbita argyrosperma subsp. sororia] >KAG7037570.1 putative cyclic nucleotide-gated ion channel 14 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1191.4 bits (3081), Expect = 0.0e+00
Identity = 620/738 (84.01%), Postives = 644/738 (87.26%), Query Frame = 0

Query: 1   MELKKAKFVRFYSNEKKNIELPWEKNDGKKRNVEFPLPVFKMG-----KKLENESKIMNS 60
           MELKK KFVRFYSNEKKN+ELPWEKNDGK+R  EF LP FKMG       + ++ KI  S
Sbjct: 1   MELKKGKFVRFYSNEKKNLELPWEKNDGKRR-FEFALPFFKMGTAPLTNAVGDKGKI--S 60

Query: 61  DSSGRSKVFVESNGPQEEKNKRILNPESERILRWNRVFLFSCLTALFVDPLFFYLPSVIH 120
              GR K+FVE+N   E + K ILNPESE ILRWNRVFLFSCLTALFVDPLFFYLPSVIH
Sbjct: 61  SDLGRFKIFVENN---EAQQKWILNPESETILRWNRVFLFSCLTALFVDPLFFYLPSVIH 120

Query: 121 HNRSSCMTTDFNLGIVVTVFRTFADVFYLLHMILKFRIAYVSPTSRVFGK---------- 180
           HNRSSCMTTDFNLGI VTVFRTFADVFYL HM+LKFR+AYVSPTSRVFGK          
Sbjct: 121 HNRSSCMTTDFNLGIAVTVFRTFADVFYLFHMVLKFRMAYVSPTSRVFGKGELVTDPKKI 180

Query: 181 -----------------------------AIRSSHADHTNNTLVLIVLLQYIPRFYLIFP 240
                                        AIRSSHADHTNNTLVLIVLLQYIPRFYLIFP
Sbjct: 181 AKRYLTSDFCIDLIASLPLPQIMIWFIMPAIRSSHADHTNNTLVLIVLLQYIPRFYLIFP 240

Query: 241 LSSHIIKTTGVVTKTAWAGAAYNLVLYMLASHILGAAWYLLSVERHAMCWKFACRNEFSP 300
           LSSHIIKTTGVVTKTAWAGAAYNLVLYMLASHILGAAWYLLSVERHAMCWKF CR EFSP
Sbjct: 241 LSSHIIKTTGVVTKTAWAGAAYNLVLYMLASHILGAAWYLLSVERHAMCWKFTCRREFSP 300

Query: 301 MKCFLDYLDCGTLNHVDRRIWEVNTTVFSQCSPDEDVIFNYGIFANAIAKDVISSRFLQK 360
           MKC LDYLDCGTLN VDRRIWEVNTTVF+QCSPDEDV+FNYGIFANAI+K+VIS  F QK
Sbjct: 301 MKCHLDYLDCGTLNDVDRRIWEVNTTVFTQCSPDEDVVFNYGIFANAISKNVISFGFAQK 360

Query: 361 YFYCLWWGLQNLRYEYICDSYGQGLETSIYMGETLFAILIAIMGLVLFAHLIGNMQTYLQ 420
           YFYCLWWGLQNL       SYGQGLETS ++GET FAILIAIMGLVLFAHLIGNMQTYLQ
Sbjct: 361 YFYCLWWGLQNL------SSYGQGLETSTFIGETSFAILIAIMGLVLFAHLIGNMQTYLQ 420

Query: 421 SITVRLEEWRVKRRDTEEWMKHRQLPRDLQERVRRFVQYKWLATRGVDEESILQGLPTDL 480
           SITVRLEEWRVKRRDTEEWM HRQLP DLQERVRRFVQYKWLATRGVDEESILQ LPTDL
Sbjct: 421 SITVRLEEWRVKRRDTEEWMNHRQLPEDLQERVRRFVQYKWLATRGVDEESILQALPTDL 480

Query: 481 RRDIQRHLCLDLVRRVPFFAQMDDQLLDAICERLASSLCTQRTYIVREGDPVTEMLFIIR 540
           RRDIQRHLCLDLVRRVPFFAQMDDQLLDAICERLASSLCTQ T+IVREGDPVTEM FIIR
Sbjct: 481 RRDIQRHLCLDLVRRVPFFAQMDDQLLDAICERLASSLCTQGTFIVREGDPVTEMFFIIR 540

Query: 541 GMLESSTTDGGRSGFFNSITLRPGDFCGEELLAWALLPKSSISLPSSTRTVRAITEVEAF 600
           GMLESSTTDGGRSGFFNSITLRPGDFCGEELLAWALLPKSSISLPSSTRTVRAITEVEAF
Sbjct: 541 GMLESSTTDGGRSGFFNSITLRPGDFCGEELLAWALLPKSSISLPSSTRTVRAITEVEAF 600

Query: 601 ALRAEDLKFVANQFRRLHSKKLQHTFRFYSYHWRTWAACFIQAAWRRFKRRIIAKSLSLQ 660
           ALRAEDLKFVANQFRRLHSKKLQHTFRFYSYHWRTWAACFIQAAWRRFKRRIIAK+LS+Q
Sbjct: 601 ALRAEDLKFVANQFRRLHSKKLQHTFRFYSYHWRTWAACFIQAAWRRFKRRIIAKTLSIQ 660

Query: 661 ESFSMTPEKPVAEETEQEEEEHNTPRSNYSQAKQNLGVTILASRFAANTRRGAQKLKDIN 695
           ESFS+ P+KP+A+E E EEEEHN PRSNYSQAKQNLGVTILASRFAANTRRGAQKLKD+N
Sbjct: 661 ESFSLNPKKPMADEDELEEEEHN-PRSNYSQAKQNLGVTILASRFAANTRRGAQKLKDVN 720

BLAST of Lsi04G022030 vs. TAIR 10
Match: AT2G24610.1 (cyclic nucleotide-gated channel 14 )

HSP 1 Score: 865.9 bits (2236), Expect = 2.2e-251
Identity = 469/746 (62.87%), Postives = 541/746 (72.52%), Query Frame = 0

Query: 1   MELKKAKFVRFYSNEKKNIELPWEKNDGKKRNVEFPLPVFK-----------MGKKLENE 60
           ME K+   VRFY +EK+ IE+      G+KR     +P+FK           + KK +  
Sbjct: 1   MEFKRDNTVRFYGDEKQTIEV------GEKR-----VPLFKSTTAPFMKQEVLPKKSKTR 60

Query: 61  SKIMNSDSSGRSKVFVESNGPQEEKNKRILNPESERILRWNRVFLFSCLTALFVDPLFFY 120
            KI      GR KVF E+    E +  +IL+P  + +L+WNRVFLF CL AL+VDPLFF+
Sbjct: 61  LKI---PRFGRFKVFPEN---FEIERDKILDPGGDAVLQWNRVFLFWCLVALYVDPLFFF 120

Query: 121 LPSVIHHNRSSCMTTDFNLGIVVTVFRTFADVFYLLHMILKFRIAYVSPTSRVFGK---- 180
           L SV    RSSCMTTD  LGIV+T FRT AD+FY+LH+++KFR AYVS TSRVFG+    
Sbjct: 121 LSSVKRIGRSSCMTTDLKLGIVITFFRTLADLFYVLHIVIKFRTAYVSRTSRVFGRGELV 180

Query: 181 -----------------------------------AIRSSHADHTNNTLVLIVLLQYIPR 240
                                              +IRSSH+DHT N LVLIVL+QYIPR
Sbjct: 181 KDPKLIARRYLRSDFIVDLIACLPLPQIVSWFILPSIRSSHSDHTTNALVLIVLVQYIPR 240

Query: 241 FYLIFPLSSHIIKTTGVVTKTAWAGAAYNLVLYMLASHILGAAWYLLSVERHAMCWKFAC 300
            YLIFPLS+ IIK TGVVT TAWAGAAYNL+ YMLASHILG+AWYLLS+ER A CWK  C
Sbjct: 241 LYLIFPLSAEIIKATGVVTTTAWAGAAYNLLQYMLASHILGSAWYLLSIERQATCWKAEC 300

Query: 301 RNEFSPMKCFLDYLDCGTLNHVDRRIWEVNTTVFSQCSPDEDVIFNYGIFANAIAKDVIS 360
             E  P++C  D+ DCGTL+  DR  W+  T VFS C P  ++ F +GIFA+A+ K+V+S
Sbjct: 301 HKESVPLQCVTDFFDCGTLHRDDRNNWQNTTVVFSNCDPSNNIQFTFGIFADALTKNVVS 360

Query: 361 SRFLQKYFYCLWWGLQNLRYEYICDSYGQGLETSIYMGETLFAILIAIMGLVLFAHLIGN 420
           S FL+KY YCLW+GLQNL       SYGQ L TS  + ET+FAIL+AI GLVLFA LIGN
Sbjct: 361 SPFLEKYLYCLWFGLQNL------SSYGQNLSTSTSVLETMFAILVAIFGLVLFALLIGN 420

Query: 421 MQTYLQSITVRLEEWRVKRRDTEEWMKHRQLPRDLQERVRRFVQYKWLATRGVDEESILQ 480
           MQTYLQSITVRLEEWR+KRRDTEEWM HR LP++L+ERVRRFVQYKWLATRGVDEE+IL 
Sbjct: 421 MQTYLQSITVRLEEWRLKRRDTEEWMGHRLLPQNLRERVRRFVQYKWLATRGVDEETILH 480

Query: 481 GLPTDLRRDIQRHLCLDLVRRVPFFAQMDDQLLDAICERLASSLCTQRTYIVREGDPVTE 540
            LP DLRRDIQRHLCLDLVRRVP FAQMDDQLLDAICERLASSL TQ  YIVREGDPVTE
Sbjct: 481 SLPADLRRDIQRHLCLDLVRRVPLFAQMDDQLLDAICERLASSLSTQGNYIVREGDPVTE 540

Query: 541 MLFIIRGMLESSTTDGGRSGFFNSITLRPGDFCGEELLAWALLPKSSISLPSSTRTVRAI 600
           MLFIIRG LESSTT+GGR+GFFNSITLRPGDFCGEELLAWALLPKS+++LPSSTRTVRA+
Sbjct: 541 MLFIIRGKLESSTTNGGRTGFFNSITLRPGDFCGEELLAWALLPKSTVNLPSSTRTVRAL 600

Query: 601 TEVEAFALRAEDLKFVANQFRRLHSKKLQHTFRFYSYHWRTWAACFIQAAWRRFKRRIIA 660
            EVEAFAL+A DLKFVANQFRRLHSKKLQHTFR+YS+ WRTWAACF+Q AWRR+KR+ +A
Sbjct: 601 EEVEAFALQAGDLKFVANQFRRLHSKKLQHTFRYYSHQWRTWAACFVQVAWRRYKRKKLA 660

Query: 661 KSLSLQESFSMTPEKPVAEETEQEEEEHN-------TPRSNYSQAKQNLGVTILASRFAA 690
           KSLSL ESFS   E+        EE  H          R + S  K +   TILASRFA 
Sbjct: 661 KSLSLAESFSSYDEEEAVAVAATEEMSHEGEAQSGAKARHHTSNVKPHFAATILASRFAK 720

BLAST of Lsi04G022030 vs. TAIR 10
Match: AT4G30360.1 (cyclic nucleotide-gated channel 17 )

HSP 1 Score: 826.6 bits (2134), Expect = 1.5e-239
Identity = 441/737 (59.84%), Postives = 531/737 (72.05%), Query Frame = 0

Query: 1   MELKKAKFVRFYSNEKKNIELPWEKNDGKKRNVEFPLPVFKMGKKLENESKIMNSDSSGR 60
           MEL+K K + FYS  K++ E  W  ND   ++ +  LP       L   +++  +D+S  
Sbjct: 1   MELRKDKLLMFYSEGKESKEAKWAVNDPMSKSYKLSLPSALRPDNLLPGNRLRYTDAS-- 60

Query: 61  SKVFVESNGPQEEKNKRILNPESERILRWNRVFLFSCLTALFVDPLFFYLPSVIHHNRSS 120
                +S   +    K IL+P SE +L+WN VF+ SC+ ALF+DPL+F++P++       
Sbjct: 61  -----KSKSSKVSWYKTILDPGSEIVLKWNWVFIVSCMVALFIDPLYFFVPAIGGDKNYP 120

Query: 121 CMTTDFNLGIVVTVFRTFADVFYLLHMILKFRIAYVSPTS--RVFG--------KAI--- 180
           C  TD +L I+VT FRT AD+FYLLH+ +KFR  +++P S  RVFG        KAI   
Sbjct: 121 CARTDTSLSILVTFFRTIADLFYLLHIFIKFRTGFIAPNSSTRVFGRGELVMDPKAIAWR 180

Query: 181 ----------------------------RSSHADHTNNTLVLIVLLQYIPRFYLIFPLSS 240
                                       +S   DH NN + LIVLLQYIPRFYLI PLSS
Sbjct: 181 YIKSDFIIDLIATLPLPQIVIWFVISTTKSYRFDHNNNAIALIVLLQYIPRFYLIIPLSS 240

Query: 241 HIIKTTGVVTKTAWAGAAYNLVLYMLASHILGAAWYLLSVERHAMCWKFACRNEFSPMKC 300
            I+K TGVVTKTAWAGAAYNL+LYMLASH+LGAAWY+LSV+R+  CWK  C  E   + C
Sbjct: 241 QIVKATGVVTKTAWAGAAYNLLLYMLASHVLGAAWYILSVDRYTSCWKSRCNGEAGQVNC 300

Query: 301 FLDYLDCGTLNHVDRRIWEVNTTVFSQCSPDEDVIFNYGIFANAIAKDVISSRFLQKYFY 360
            L YLDC ++   ++  W   T VF  C    +  F YGIF NAI K+V+SS+F ++YFY
Sbjct: 301 QLYYLDCDSMYDNNQMTWANVTKVFKLCDA-RNGEFKYGIFGNAITKNVVSSQFFERYFY 360

Query: 361 CLWWGLQNLRYEYICDSYGQGLETSIYMGETLFAILIAIMGLVLFAHLIGNMQTYLQSIT 420
           CLWWGLQ L       SYGQ L T+++MGET FA+LIAI GLVLFAHLIGNMQTYLQS+T
Sbjct: 361 CLWWGLQQL------SSYGQNLSTTMFMGETTFAVLIAIFGLVLFAHLIGNMQTYLQSLT 420

Query: 421 VRLEEWRVKRRDTEEWMKHRQLPRDLQERVRRFVQYKWLATRGVDEESILQGLPTDLRRD 480
           VRLEEWR+K+RDTEEWM+HRQLP +L+ RVRR+ QYKWLATRGVDEE +LQ LPTDLRRD
Sbjct: 421 VRLEEWRLKKRDTEEWMRHRQLPEELRNRVRRYEQYKWLATRGVDEEVLLQSLPTDLRRD 480

Query: 481 IQRHLCLDLVRRVPFFAQMDDQLLDAICERLASSLCTQRTYIVREGDPVTEMLFIIRGML 540
           IQRHLCLDLVRRVPFF+QMDDQLLDAICERL SSLCT+ TY+VREGD ++EMLFIIRG L
Sbjct: 481 IQRHLCLDLVRRVPFFSQMDDQLLDAICERLVSSLCTEGTYLVREGDLISEMLFIIRGRL 540

Query: 541 ESSTTDGGRSGFFNSITLRPGDFCGEELLAWALLPKSSISLPSSTRTVRAITEVEAFALR 600
           ESSTT+GGR+GFFNSI LRPGDFCGEELL+WALLPKS+++LPSSTRTVRA+ EVEAFALR
Sbjct: 541 ESSTTNGGRTGFFNSIILRPGDFCGEELLSWALLPKSTLNLPSSTRTVRALVEVEAFALR 600

Query: 601 AEDLKFVANQFRRLHSKKLQHTFRFYSYHWRTWAACFIQAAWRRFKRRIIAKSLSLQESF 660
           AEDLKFVANQFRRLHSKKLQHTFRFYS+HWRTWAACFIQAAWRR+KRR++  +L+  ES 
Sbjct: 601 AEDLKFVANQFRRLHSKKLQHTFRFYSHHWRTWAACFIQAAWRRYKRRVMENNLTAIESM 660

Query: 661 SMTPEKPVAEETEQEEEEHNTPRSNYSQAKQNLGVTILASRFAANTRRG--AQKLKDINL 695
               E  V EE    EEE     S   + K NLGV +LASRFAANTRRG  AQ++KD+ L
Sbjct: 661 E-NEEGEVGEELVVVEEEECVEES--PRTKMNLGVMVLASRFAANTRRGVAAQRVKDVEL 720

BLAST of Lsi04G022030 vs. TAIR 10
Match: AT5G14870.1 (cyclic nucleotide-gated channel 18 )

HSP 1 Score: 709.5 bits (1830), Expect = 2.6e-204
Identity = 377/676 (55.77%), Postives = 466/676 (68.93%), Query Frame = 0

Query: 77  RILNPESERILRWNRVFLFSCLTALFVDPLFFYLPSVIHHNRSSCMTTDFNLGIVVTVFR 136
           +IL+P+S  +  WN VFL + + ALF+DP +FY+P V      +C++ D +L   VT FR
Sbjct: 39  QILDPDSNIVTYWNHVFLITSILALFLDPFYFYVPYV---GGPACLSIDISLAATVTFFR 98

Query: 137 TFADVFYLLHMILKFRIAYVSPTSRVFGK------------------------------- 196
           T AD+F+LLH+ +KFR A+V+ +SRVFG+                               
Sbjct: 99  TVADIFHLLHIFMKFRTAFVARSSRVFGRGELVMDSREIAMRYLKTDFLIDVAAMLPLPQ 158

Query: 197 --------AIRSSHADHTNNTLVLIVLLQYIPRFYLIFPLSSHIIKTTGVVTKTAWAGAA 256
                   A  +  A+H N+TL LIVL+QYIPR ++IFPL+  IIKTTG + KTAWAGAA
Sbjct: 159 LVIWLVIPAATNGTANHANSTLALIVLVQYIPRSFIIFPLNQRIIKTTGFIAKTAWAGAA 218

Query: 257 YNLVLYMLASHILGAAWYLLSVERHAMCWKFACR--NEFSPMKCFLDYLDCGTLNHVDRR 316
           YNL+LY+LASH+LGA WYL S+ R   CW   C+  N    + C   +LDC +L   +R+
Sbjct: 219 YNLLLYILASHVLGAMWYLSSIGRQFSCWSNVCKKDNALRVLDCLPSFLDCKSLEQPERQ 278

Query: 317 IWEVNTTVFSQCSPDEDVI-FNYGIFANAIAKDVISSRFLQKYFYCLWWGLQNLRYEYIC 376
            W+  T V S C        F +G+FA A    V ++ F+ KY YCLWWGL+NL      
Sbjct: 279 YWQNVTQVLSHCDATSSTTNFKFGMFAEAFTTQVATTDFVSKYLYCLWWGLRNL------ 338

Query: 377 DSYGQGLETSIYMGETLFAILIAIMGLVLFAHLIGNMQTYLQSITVRLEEWRVKRRDTEE 436
            SYGQ + TS+Y+GETLF I I I GL+LF  LIGNMQ+ LQS++VR+EEWRVKRRDTEE
Sbjct: 339 SSYGQNITTSVYLGETLFCITICIFGLILFTLLIGNMQSSLQSMSVRVEEWRVKRRDTEE 398

Query: 437 WMKHRQLPRDLQERVRRFVQYKWLATRGVDEESILQGLPTDLRRDIQRHLCLDLVRRVPF 496
           WM+HRQLP +LQERVRRFVQYKWLATRGVDEESIL  LPTDLRR+IQRHLCL LVRRVPF
Sbjct: 399 WMRHRQLPPELQERVRRFVQYKWLATRGVDEESILHSLPTDLRREIQRHLCLSLVRRVPF 458

Query: 497 FAQMDDQLLDAICERLASSLCTQRTYIVREGDPVTEMLFIIRGMLESSTTDGGRSGFFNS 556
           F+QMDDQLLDAIC  L SSL T  TYI REGDPV EMLF+IRG +ESSTT+GGRSGFFNS
Sbjct: 459 FSQMDDQLLDAICGCLVSSLSTAGTYIFREGDPVNEMLFVIRGQIESSTTNGGRSGFFNS 518

Query: 557 ITLRPGDFCGEELLAWALLPKSSISLPSSTRTVRAITEVEAFALRAEDLKFVANQFRRLH 616
            TLRPGDFCGEELL WAL+P S+++LPSSTR+VRA++EVEAFAL AEDLKFVA+QF+RL 
Sbjct: 519 TTLRPGDFCGEELLTWALMPNSTLNLPSSTRSVRALSEVEAFALSAEDLKFVAHQFKRLQ 578

Query: 617 SKKLQHTFRFYSYHWRTWAACFIQAAWRRFKRRIIAKSLSLQESFS-MTPEKPVAEETEQ 676
           SKKLQH FR+YS+ WR W ACF+Q+AWRR+KRR +AK LSL ES     P++    E ++
Sbjct: 579 SKKLQHAFRYYSHQWRAWGACFVQSAWRRYKRRKLAKELSLHESSGYYYPDETGYNEEDE 638

Query: 677 EEEE--HNTPRSNYSQAKQNLGVTILASRFAANTRRGAQK--------LKD-----INLS 695
           E  E  + +     S    NLG TILAS+FAANTRRG  +         KD     + + 
Sbjct: 639 ETREYYYGSDEEGGSMDNTNLGATILASKFAANTRRGTNQKASSSSTGKKDGSSTSLKMP 698

BLAST of Lsi04G022030 vs. TAIR 10
Match: AT3G48010.1 (cyclic nucleotide-gated channel 16 )

HSP 1 Score: 693.7 bits (1789), Expect = 1.5e-199
Identity = 373/688 (54.22%), Postives = 457/688 (66.42%), Query Frame = 0

Query: 68  NGPQEEKNKRI--------LNPESERILRWNRVFLFSCLTALFVDPLFFYLPSVIHHNRS 127
           N P  ++ + I        L+P  + I RWN +FL +CL ALF+DPL+FYLP V     +
Sbjct: 26  NDPNNQRRRSIFSKLRDKTLDPGGDLITRWNHIFLITCLLALFLDPLYFYLPIV--QAGT 85

Query: 128 SCMTTDFNLGIVVTVFRTFADVFYLLHMILKFRIAYVSPTSRVFGK----------AIR- 187
           +CM+ D   GI VT FR  AD+ +L+H++LKF+ A+VS +SRVFG+          AIR 
Sbjct: 86  ACMSIDVRFGIFVTCFRNLADLSFLIHILLKFKTAFVSKSSRVFGRGELVMDRREIAIRY 145

Query: 188 -----------------------------SSHADHTNNTLVLIVLLQYIPRFYLIFPLSS 247
                                          +A H N+TL LIVL+QY+PRF ++ PL+ 
Sbjct: 146 LKSEFVIDLAATLPLPQIMIWFVIPNAGEFRYAAHQNHTLSLIVLIQYVPRFLVMLPLNR 205

Query: 248 HIIKTTGVVTKTAWAGAAYNLVLYMLASHILGAAWYLLSVERHAMCWKFACRNEFSPM-- 307
            IIK TGV  KTAW+GAAYNL+LY+L SH+LG+ WY+LS++R   CW+  C  E +    
Sbjct: 206 RIIKATGVAAKTAWSGAAYNLILYLLVSHVLGSVWYVLSIQRQHECWRRECIKEMNATHS 265

Query: 308 -KCFLDYLDCGTLNHVDRRIWEVNTTVFSQCSP--DEDVIFNYGIFANAIAKDVISSRFL 367
             C L +LDCG+L+   R+ W   T V S C    D+D  F +G+F +A   DV SS F 
Sbjct: 266 PSCSLLFLDCGSLHDPGRQAWMRITRVLSNCDARNDDDQHFQFGMFGDAFTNDVTSSPFF 325

Query: 368 QKYFYCLWWGLQNLRYEYICDSYGQGLETSIYMGETLFAILIAIMGLVLFAHLIGNMQTY 427
            KYFYCLWWGL+NL       SYGQ L  S    ET+F+  I + GLV F+HLIGN+Q Y
Sbjct: 326 DKYFYCLWWGLRNL------SSYGQSLAASTLSSETIFSCFICVAGLVFFSHLIGNVQNY 385

Query: 428 LQSITVRLEEWRVKRRDTEEWMKHRQLPRDLQERVRRFVQYKWLATRGVDEESILQGLPT 487
           LQS T RL+EWRV+RRDTEEWM+HRQLP +LQERVRRFVQYKWL TRGVDEE+IL+ LP 
Sbjct: 386 LQSTTARLDEWRVRRRDTEEWMRHRQLPDELQERVRRFVQYKWLTTRGVDEEAILRALPL 445

Query: 488 DLRRDIQRHLCLDLVRRVPFFAQMDDQLLDAICERLASSLCTQRTYIVREGDPVTEMLFI 547
           DLRR IQRHLCL LVRRVPFFAQMDDQLLDAICERL  SL T+ TY++REGDPV EMLFI
Sbjct: 446 DLRRQIQRHLCLALVRRVPFFAQMDDQLLDAICERLVPSLNTKDTYVIREGDPVNEMLFI 505

Query: 548 IRGMLESSTTDGGRSGFFNSITLRPGDFCGEELLAWALLPKSSISLPSSTRTVRAITEVE 607
           IRG +ESSTTDGGRSGFFNSITLRPGDFCGEELL WAL+P  + +LP STRTVR ++EVE
Sbjct: 506 IRGQMESSTTDGGRSGFFNSITLRPGDFCGEELLTWALVPNINHNLPLSTRTVRTLSEVE 565

Query: 608 AFALRAEDLKFVANQFRRLHSKKLQHTFRFYSYHWRTWAACFIQAAWRRFKRRIIAKSLS 667
           AFALRAEDLKFVANQFRRLHSKKLQH FR+YS+ WR W  CFIQAAWRR+ +R +A  L+
Sbjct: 566 AFALRAEDLKFVANQFRRLHSKKLQHAFRYYSHQWRAWGTCFIQAAWRRYMKRKLAMELA 625

Query: 668 LQE---SFSMTPEKPVAEETEQEEEEHNTPRSNYSQAKQNLGVTILASRFAANTRRGA-- 689
            QE    +    +     E +  E  +N    N S   QNL  TILAS+FAANT+RG   
Sbjct: 626 RQEEEDDYFYDDDGDYQFEEDMPESNNNNGDEN-SSNNQNLSATILASKFAANTKRGVLG 685

BLAST of Lsi04G022030 vs. TAIR 10
Match: AT2G28260.1 (cyclic nucleotide-gated channel 15 )

HSP 1 Score: 644.4 bits (1661), Expect = 1.0e-184
Identity = 349/692 (50.43%), Postives = 443/692 (64.02%), Query Frame = 0

Query: 40  FKMGKKLENESKIMNSDSSGRSKVFVESNGPQEEKNK-RILNPESERILRWNRVFLFSCL 99
           FK+     N  K+M+     ++KV         E+ K +IL+P  + I RWN++FL +CL
Sbjct: 29  FKINGTQINNVKMMSKGKFLKAKVLSRVFSEDLERVKTKILDPRGQTIRRWNKIFLIACL 88

Query: 100 TALFVDPLFFYLPSVIHHNRSSCMTTDFNLGIVVTVFRTFADVFYLLHMILKFRIAYVSP 159
            +LFVDPLFF+LP +      +C+T    L +V+T+ R+ AD FY+  ++++FR AY++P
Sbjct: 89  VSLFVDPLFFFLPVM---RNEACITIGVRLEVVLTLIRSLADAFYIAQILIRFRTAYIAP 148

Query: 160 TSRVFGKA---------------------------------------IRSSHADHTNNTL 219
            SRVFG+                                        +R S   +T N L
Sbjct: 149 PSRVFGRGELVIDSRKIAWRYLHKSFWIHLVAALPLPQVLIWIIIPNLRGSPMTNTKNVL 208

Query: 220 VLIVLLQYIPRFYLIFPLSSHIIKTTGVVTKTAWAGAAYNLVLYMLASHILGAAWYLLSV 279
             I++ QY+PR +LIFPLS  IIK TGVVT+TAWAGAAYNL+LYMLASH+LGA WYLL+V
Sbjct: 209 RFIIIFQYVPRMFLIFPLSRQIIKATGVVTETAWAGAAYNLMLYMLASHVLGACWYLLAV 268

Query: 280 ERHAMCWKFACRNEFSPMKCFLDYLDCGTLNHVDRRIWEVNTTVFSQCSPDEDVIFNYGI 339
           ER   CW+ AC  E     C   + +C  L    R  W   + + + C P     + +GI
Sbjct: 269 ERQEACWRHACNIE--KQICQYRFFECRRLEDPQRNSWFEWSNITTICKP-ASKFYEFGI 328

Query: 340 FANAIAKDVISSRFLQKYFYCLWWGLQNLRYEYICDSYGQGLETSIYMGETLFAILIAIM 399
           F +A+   V SS+F+ KYFYCLWWGL+NL       S GQ L TS Y GE LFAI+IA +
Sbjct: 329 FGDAVTSTVTSSKFINKYFYCLWWGLKNL------SSLGQNLATSTYAGEILFAIIIATL 388

Query: 400 GLVLFAHLIGNMQTYLQSITVRLEEWRVKRRDTEEWMKHRQLPRDLQERVRRFVQYKWLA 459
           GLVLFA LIGNMQTYLQS T+RLEEWR++R DTE+WM HRQLP +L++ VR++ QYKWLA
Sbjct: 389 GLVLFALLIGNMQTYLQSTTMRLEEWRIRRTDTEQWMHHRQLPPELRQAVRKYDQYKWLA 448

Query: 460 TRGVDEESILQGLPTDLRRDIQRHLCLDLVRRVPFFAQMDDQLLDAICERLASSLCTQRT 519
           TRGVDEE++L  LP DLRRDI+RHLC DLVRRVP F QMD+++LDAICERL  +LCT+ T
Sbjct: 449 TRGVDEEALLISLPLDLRRDIKRHLCFDLVRRVPLFDQMDERMLDAICERLKPALCTEGT 508

Query: 520 YIVREGDPVTEMLFIIRGMLESSTTDGGRSGFFNSITLRPGDFCGEELLAWALLPKSSIS 579
           ++VREGDPV EMLFIIRG L+S TT+GGR+GFFNS  + PGDFCGEELL WAL P+  + 
Sbjct: 509 FLVREGDPVNEMLFIIRGHLDSYTTNGGRTGFFNSCLIGPGDFCGEELLTWALDPRPVVI 568

Query: 580 LPSSTRTVRAITEVEAFALRAEDLKFVANQFRRLHSKKLQHTFRFYSYHWRTWAACFIQA 639
           LPSSTRTV+AI EVEAFAL+AEDL+FVA+QFRRLH+K+L+H FRFYS+ WRTWAACFIQA
Sbjct: 569 LPSSTRTVKAICEVEAFALKAEDLQFVASQFRRLHTKQLRHKFRFYSHQWRTWAACFIQA 628

Query: 640 AWRRFKRRIIAKSLSLQESFSMTPEKPVAEETEQEEEEHNTPRSNYSQAKQNLGVTILAS 692
           AWRR ++R     L  +E F    E   A                            LA 
Sbjct: 629 AWRRHRKRKYKTELRAKEEFHYRFEAATAR---------------------------LAV 678

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SJA43.1e-25062.87Probable cyclic nucleotide-gated ion channel 14 OS=Arabidopsis thaliana OX=3702 ... [more]
Q8L7Z02.1e-23859.84Cyclic nucleotide-gated ion channel 17 OS=Arabidopsis thaliana OX=3702 GN=CNGC17... [more]
Q9LEQ33.7e-20355.77Cyclic nucleotide-gated ion channel 18 OS=Arabidopsis thaliana OX=3702 GN=CNGC18... [more]
Q9SU642.1e-19854.22Probable cyclic nucleotide-gated ion channel 16 OS=Arabidopsis thaliana OX=3702 ... [more]
A0A072VMJ31.0e-18951.94Protein CNGC15c OS=Medicago truncatula OX=3880 GN=CNGC15C PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A5A7T7B40.0e+0085.97Putative cyclic nucleotide-gated ion channel 14 isoform X1 OS=Cucumis melo var. ... [more]
A0A1S3CQV90.0e+0085.97probable cyclic nucleotide-gated ion channel 14 isoform X1 OS=Cucumis melo OX=36... [more]
A0A0A0LGT40.0e+0086.10Cyclic nucleotide-binding domain-containing protein OS=Cucumis sativus OX=3659 G... [more]
A0A6J1J1M10.0e+0083.88probable cyclic nucleotide-gated ion channel 14 isoform X1 OS=Cucurbita maxima O... [more]
A0A6J1CLB20.0e+0083.15probable cyclic nucleotide-gated ion channel 14 OS=Momordica charantia OX=3673 G... [more]
Match NameE-valueIdentityDescription
XP_038896574.10.0e+0089.22probable cyclic nucleotide-gated ion channel 14 isoform X1 [Benincasa hispida][more]
XP_008466292.10.0e+0085.97PREDICTED: probable cyclic nucleotide-gated ion channel 14 isoform X1 [Cucumis m... [more]
XP_004136445.10.0e+0086.10probable cyclic nucleotide-gated ion channel 14 isoform X1 [Cucumis sativus] >KG... [more]
XP_023524088.10.0e+0084.15probable cyclic nucleotide-gated ion channel 14 isoform X1 [Cucurbita pepo subsp... [more]
KAG6608211.10.0e+0084.01putative cyclic nucleotide-gated ion channel 14, partial [Cucurbita argyrosperma... [more]
Match NameE-valueIdentityDescription
AT2G24610.12.2e-25162.87cyclic nucleotide-gated channel 14 [more]
AT4G30360.11.5e-23959.84cyclic nucleotide-gated channel 17 [more]
AT5G14870.12.6e-20455.77cyclic nucleotide-gated channel 18 [more]
AT3G48010.11.5e-19954.22cyclic nucleotide-gated channel 16 [more]
AT2G28260.11.0e-18450.43cyclic nucleotide-gated channel 15 [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000595Cyclic nucleotide-binding domainSMARTSM00100cnmp_10coord: 454..585
e-value: 6.3E-13
score: 58.9
IPR000595Cyclic nucleotide-binding domainPFAMPF00027cNMP_bindingcoord: 478..565
e-value: 3.6E-8
score: 33.4
IPR000595Cyclic nucleotide-binding domainPROSITEPS50042CNMP_BINDING_3coord: 454..584
score: 10.1758
IPR000595Cyclic nucleotide-binding domainCDDcd00038CAP_EDcoord: 454..585
e-value: 5.65369E-19
score: 80.833
IPR014710RmlC-like jelly roll foldGENE3D2.60.120.10Jelly Rollscoord: 452..572
e-value: 1.7E-38
score: 133.1
IPR005821Ion transport domainPFAMPF00520Ion_transcoord: 89..381
e-value: 1.9E-21
score: 76.4
NoneNo IPR availableGENE3D1.10.287.630Helix hairpin bincoord: 382..445
e-value: 1.5E-19
score: 72.0
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 621..648
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 674..694
NoneNo IPR availablePANTHERPTHR45651:SF9CYCLIC NUCLEOTIDE-GATED ION CHANNEL 14-RELATEDcoord: 43..165
coord: 166..691
NoneNo IPR availablePANTHERPTHR45651CYCLIC NUCLEOTIDE-GATED ION CHANNEL 15-RELATED-RELATEDcoord: 43..165
coord: 166..691
NoneNo IPR availableSUPERFAMILY81324Voltage-gated potassium channelscoord: 85..376
IPR018490Cyclic nucleotide-binding-likeSUPERFAMILY51206cAMP-binding domain-likecoord: 384..576

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi04G022030.1Lsi04G022030.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0071805 potassium ion transmembrane transport
biological_process GO:0006811 ion transport
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0016020 membrane
molecular_function GO:0005249 voltage-gated potassium channel activity
molecular_function GO:0005216 ion channel activity