Lsi04G021570 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi04G021570
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	dnaJ homolog subfamily B member 4
Location	chr04: 28636644 .. 28638098 (-)
RNA-Seq Expression	Lsi04G021570
Synteny	Lsi04G021570

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGATCAGAAACCAATATCCAAACTGTTTGTATAATCAGATAAGTTTTATAATACAGGTGTTGAGTGACCCCCAGAAGAAGGCTATTTATGATCAATATGGTGAAGAAGGGTTGAAAGATATGCCACCACCAGGCAGTGCAAGCTTCCCATTTGGAAACGGTGGCGGCGGCGGGTCGAGCGGGTTTAATCCAAGGAATGCAGAGGATATTTTTGCAGAATTTTTTGGGAGCAGCCCTTTTGGGTTTGGTTCATCAGGACCAGGGAAGTCTATGAGGTACCAGTCAGAGGGAGGAGTTTTTGGGGGATTTGGTGGAAGTGAAAATATTTTCAGAACATATAGTGAAAATATAACACCAAAAAAACCTCCACCAGTTGAGAGCAAATTGCCCTGCACTCTTGAGGAGCTGTATTCAGGATCTACAAGGAAAATGAAAATTTCAAGAACAGTAGTTGATGCAAACGGGTATGCTATTTTCATCTGTTTTCACAGTAATCGGCACTTCCATTTTGTCTCTCTTTTTCTATAAGAAACTGTAGTATAACAAGATAAGTAAAAAGGAGTTACAAAAGAAGAGTCTTCCATGAAATTAACCATAAAAGACTGTAATTACTGCCGAAGAATTTCTTATGATATGGCTTGTGTGCTGTACACTATTATGAATAACAATCATAACATTTTCTTTTGTCTGAGATTCTGAGAGGATGCGATAAATGTTGAGACTATGATCCATAGAATATTAACCAGGAAAATTCTAGTATCGGATTTGTGGTTGCACTCTGATGATACTTTTAGAGGATGAAAATACTGGTTTATTTGGCTAAACATTGTTTCCCTTGTGTGAGGTTGTCAGATCTACTCACTGTAATCACAAATTTACACTTTGTGGGGGTAAAAATGACCCCTGTTATAGGCTCCTGGAATTGAAGTATCAACTTCAGGATATTTTTACATTTTTCTTAGTTGGTTATCTTCTCTAATGAATTCTGTTCCATCTTATGTTTAAAAACATTACAGACGACAAGTTCCAGAGACGGAGATATTAACCATTGATGTGAAGCCCGGTTGGAAGAAAGGGACCAAAATCACCTTCCCAGATAAAGGAAATGAACAACCAAACCAACTACCCGCAGATCTGGTATTTATTATCGATGAGAAGCCCCATGATGTTTTCAAAAGAGATGGTAATGATATCATTATGAACCATAGGGTGACGTTAGCAGAGGCATTAGGCGGAACTACTATAAATCTTACCACGCTTGATGGTCGTAACCTATCAATACCCGTAATAGACATTGTTAGCCCAGGCTACGAGCTCGTGATCTCCAAAGAGGGAATGCCAATTGTAAGAGAACCTGGTAACCGGGGTGATTTGAAGATCAAATTCGATGTAAAATTCCCAACAAGATTAACCCCCGAGCAACGAGCAGGACTCAAACGTGCTTTGGGAGGTTGA

mRNA sequence

ATGATCAGAAACCAATATCCAAACTGTTTGTATAATCAGATAAGTTTTATAATACAGGTGTTGAGTGACCCCCAGAAGAAGGCTATTTATGATCAATATGGTGAAGAAGGGTTGAAAGATATGCCACCACCAGGCAGTGCAAGCTTCCCATTTGGAAACGGTGGCGGCGGCGGGTCGAGCGGGTTTAATCCAAGGAATGCAGAGGATATTTTTGCAGAATTTTTTGGGAGCAGCCCTTTTGGGTTTGGTTCATCAGGACCAGGGAAGTCTATGAGGTACCAGTCAGAGGGAGGAGTTTTTGGGGGATTTGGTGGAAGTGAAAATATTTTCAGAACATATAGTGAAAATATAACACCAAAAAAACCTCCACCAGTTGAGAGCAAATTGCCCTGCACTCTTGAGGAGCTGTATTCAGGATCTACAAGGAAAATGAAAATTTCAAGAACAGTAGTTGATGCAAACGGACGACAAGTTCCAGAGACGGAGATATTAACCATTGATGTGAAGCCCGGTTGGAAGAAAGGGACCAAAATCACCTTCCCAGATAAAGGAAATGAACAACCAAACCAACTACCCGCAGATCTGGTATTTATTATCGATGAGAAGCCCCATGATGTTTTCAAAAGAGATGGTAATGATATCATTATGAACCATAGGGTGACGTTAGCAGAGGCATTAGGCGGAACTACTATAAATCTTACCACGCTTGATGGTCGTAACCTATCAATACCCGTAATAGACATTGTTAGCCCAGGCTACGAGCTCGTGATCTCCAAAGAGGGAATGCCAATTGTAAGAGAACCTGGTAACCGGGGTGATTTGAAGATCAAATTCGATGTAAAATTCCCAACAAGATTAACCCCCGAGCAACGAGCAGGACTCAAACGTGCTTTGGGAGGTTGA

Coding sequence (CDS)

ATGATCAGAAACCAATATCCAAACTGTTTGTATAATCAGATAAGTTTTATAATACAGGTGTTGAGTGACCCCCAGAAGAAGGCTATTTATGATCAATATGGTGAAGAAGGGTTGAAAGATATGCCACCACCAGGCAGTGCAAGCTTCCCATTTGGAAACGGTGGCGGCGGCGGGTCGAGCGGGTTTAATCCAAGGAATGCAGAGGATATTTTTGCAGAATTTTTTGGGAGCAGCCCTTTTGGGTTTGGTTCATCAGGACCAGGGAAGTCTATGAGGTACCAGTCAGAGGGAGGAGTTTTTGGGGGATTTGGTGGAAGTGAAAATATTTTCAGAACATATAGTGAAAATATAACACCAAAAAAACCTCCACCAGTTGAGAGCAAATTGCCCTGCACTCTTGAGGAGCTGTATTCAGGATCTACAAGGAAAATGAAAATTTCAAGAACAGTAGTTGATGCAAACGGACGACAAGTTCCAGAGACGGAGATATTAACCATTGATGTGAAGCCCGGTTGGAAGAAAGGGACCAAAATCACCTTCCCAGATAAAGGAAATGAACAACCAAACCAACTACCCGCAGATCTGGTATTTATTATCGATGAGAAGCCCCATGATGTTTTCAAAAGAGATGGTAATGATATCATTATGAACCATAGGGTGACGTTAGCAGAGGCATTAGGCGGAACTACTATAAATCTTACCACGCTTGATGGTCGTAACCTATCAATACCCGTAATAGACATTGTTAGCCCAGGCTACGAGCTCGTGATCTCCAAAGAGGGAATGCCAATTGTAAGAGAACCTGGTAACCGGGGTGATTTGAAGATCAAATTCGATGTAAAATTCCCAACAAGATTAACCCCCGAGCAACGAGCAGGACTCAAACGTGCTTTGGGAGGTTGA

Protein sequence

MIRNQYPNCLYNQISFIIQVLSDPQKKAIYDQYGEEGLKDMPPPGSASFPFGNGGGGGSSGFNPRNAEDIFAEFFGSSPFGFGSSGPGKSMRYQSEGGVFGGFGGSENIFRTYSENITPKKPPPVESKLPCTLEELYSGSTRKMKISRTVVDANGRQVPETEILTIDVKPGWKKGTKITFPDKGNEQPNQLPADLVFIIDEKPHDVFKRDGNDIIMNHRVTLAEALGGTTINLTTLDGRNLSIPVIDIVSPGYELVISKEGMPIVREPGNRGDLKIKFDVKFPTRLTPEQRAGLKRALGG

Homology

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BLAST of Lsi04G021570 vs. ExPASy Swiss-Prot
Match: P25685 (DnaJ homolog subfamily B member 1 OS=Homo sapiens OX=9606 GN=DNAJB1 PE=1 SV=4)

HSP 1 Score: 218.0 bits (554), Expect = 1.4e-55
Identity = 127/300 (42.33%), Postives = 183/300 (61.00%), Query Frame = 0


Query: 11  YNQISFIIQVLSDPQKKAIYDQYGEEGLKDMPPPGSASFPFGNGGGGGSSGFNPRNAED- 70
           + +I+    VLSDP+K+ I+D+YGEEGLK   P G      G+GGG   + F+     D 
Sbjct: 45  FKEIAEAYDVLSDPRKREIFDRYGEEGLKGSGPSG------GSGGGANGTSFSYTFHGDP 104

Query: 71  --IFAEFFGS-SPFG--FGSSGPGKSMRYQSE-GGVFGGFGGSENI----FRTYSENITP 130
             +FAEFFG  +PF   FG     + M       G   G GG  N+     R+  E    
Sbjct: 105 HAMFAEFFGGRNPFDTFFGQRNGEEGMDIDDPFSGFPMGMGGFTNVNFGRSRSAQEPARK 164

Query: 131 KKPPPVESKLPCTLEELYSGSTRKMKISRTVVDANGRQV-PETEILTIDVKPGWKKGTKI 190
           K+ PPV   L  +LEE+YSG T+KMKIS   ++ +G+ +  E +ILTI+VK GWK+GTKI
Sbjct: 165 KQDPPVTHDLRVSLEEIYSGCTKKMKISHKRLNPDGKSIRNEDKILTIEVKKGWKEGTKI 224

Query: 191 TFPDKGNEQPNQLPADLVFIIDEKPHDVFKRDGNDIIMNHRVTLAEALGGTTINLTTLDG 250
           TFP +G++  N +PAD+VF++ +KPH++FKRDG+D+I   R++L EAL G T+N+ TLDG
Sbjct: 225 TFPKEGDQTSNNIPADIVFVLKDKPHNIFKRDGSDVIYPARISLREALCGCTVNVPTLDG 284

Query: 251 RNLSIPVIDIVSPGYELVISKEGMPIVREPGNRGDLKIKFDVKFPTRLTPEQRAGLKRAL 299
           R + +   D++ PG    +  EG+P+ + P  RGDL I+F+V FP R+    R  L++ L
Sbjct: 285 RTIPVVFKDVIRPGMRRKVPGEGLPLPKTPEKRGDLIIEFEVIFPERIPQTSRTVLEQVL 338

[Close]

BLAST of Lsi04G021570 vs. ExPASy Swiss-Prot
Match: Q5R8J8 (DnaJ homolog subfamily B member 4 OS=Pongo abelii OX=9601 GN=DNAJB4 PE=2 SV=1)

HSP 1 Score: 214.2 bits (544), Expect = 2.1e-54
Identity = 129/298 (43.29%), Postives = 181/298 (60.74%), Query Frame = 0


Query: 11  YNQISFIIQVLSDPQKKAIYDQYGEEGLKDMPPPGSASFPFGNGGGGGSSGFN-PRNAED 70
           + +++   +VLSDP+K+ IYDQ+GEEGLK     G A    G  G GG+  +    +   
Sbjct: 45  FKEVAEAYEVLSDPKKREIYDQFGEEGLK-----GGAG---GTDGQGGTFRYTFHGDPHA 104

Query: 71  IFAEFFGSSP-----FGFGSSGPGKSMRYQSEGGVFGGFGGSENIFRTYSENITP---KK 130
            FA FFG S      FG    G   S   + +G  F  FG S N +     ++ P   K+
Sbjct: 105 TFAAFFGGSNPFEIFFGRRMGGGRDSEEMEMDGDPFSAFGFSMNGYPRDRNSVGPSRLKQ 164

Query: 131 PPPVESKLPCTLEELYSGSTRKMKISRTVVDANGRQV-PETEILTIDVKPGWKKGTKITF 190
            PPV  +L  +LEE+YSG T++MKISR  ++A+GR    E +ILTI++K GWK+GTKITF
Sbjct: 165 DPPVIHELRVSLEEIYSGCTKRMKISRKRLNADGRSYRSEDKILTIEIKKGWKEGTKITF 224

Query: 191 PDKGNEQPNQLPADLVFIIDEKPHDVFKRDGNDIIMNHRVTLAEALGGTTINLTTLDGRN 250
           P +G+E PN +PAD+VFII +K H  FKRDG++II   +++L EAL G +IN+ TLDGRN
Sbjct: 225 PREGDETPNSIPADIVFIIKDKDHPKFKRDGSNIIYTAKISLREALCGCSINVPTLDGRN 284

Query: 251 LSIPVIDIVSPGYELVISKEGMPIVREPGNRGDLKIKFDVKFPTRLTPEQRAGLKRAL 299
           + + V DIV PG    I   G+P  + P  RGDL I+F+V FP  ++   +  L++ L
Sbjct: 285 IPMSVNDIVKPGMRRRIIGYGLPFPKNPDQRGDLLIEFEVSFPDTISSSSKEVLRKHL 334

[Close]

BLAST of Lsi04G021570 vs. ExPASy Swiss-Prot
Match: Q9QYJ3 (DnaJ homolog subfamily B member 1 OS=Mus musculus OX=10090 GN=Dnajb1 PE=1 SV=3)

HSP 1 Score: 213.8 bits (543), Expect = 2.7e-54
Identity = 126/300 (42.00%), Postives = 181/300 (60.33%), Query Frame = 0


Query: 11  YNQISFIIQVLSDPQKKAIYDQYGEEGLKDMPPPGSASFPFGNGGGGGSSGFNPRNAED- 70
           + +I+    VLSDP+K+ I+D+YGEEGLK   P G      G+ GG   + F+     D 
Sbjct: 45  FKEIAEAYDVLSDPRKREIFDRYGEEGLKGGSPSG------GSSGGANGTSFSYTFHGDP 104

Query: 71  --IFAEFFGS-SPFG--FGSSGPGKSMRYQSEGGVFG-GFGGSENI----FRTYSENITP 130
             +FAEFFG  +PF   FG     + M        F  G GG  N+     R   E    
Sbjct: 105 HAMFAEFFGGRNPFDTFFGQRNGEEGMDIDDTFSSFPMGMGGFTNMNFGRSRPSQEPTRK 164

Query: 131 KKPPPVESKLPCTLEELYSGSTRKMKISRTVVDANGRQV-PETEILTIDVKPGWKKGTKI 190
           K+ PPV   L  +LEE+YSG T+KMKIS   ++ +G+ +  E +ILTI+VK GWK+GTKI
Sbjct: 165 KQDPPVTHDLRVSLEEIYSGCTKKMKISHKRLNPDGKSIRNEDKILTIEVKRGWKEGTKI 224

Query: 191 TFPDKGNEQPNQLPADLVFIIDEKPHDVFKRDGNDIIMNHRVTLAEALGGTTINLTTLDG 250
           TFP +G++  N +PAD+VF++ +KPH++FKRDG+D+I   R++L EAL G T+N+ TLDG
Sbjct: 225 TFPKEGDQTSNNIPADIVFVLKDKPHNIFKRDGSDVIYPARISLREALCGCTVNVPTLDG 284

Query: 251 RNLSIPVIDIVSPGYELVISKEGMPIVREPGNRGDLKIKFDVKFPTRLTPEQRAGLKRAL 299
           R + +   D++ PG    +  EG+P+ + P  RGDL I+F+V FP R+    R  L++ L
Sbjct: 285 RTIPVVFKDVIRPGMRRKVPGEGLPLPKTPEKRGDLVIEFEVIFPERIPVSSRTILEQVL 338

[Close]

BLAST of Lsi04G021570 vs. ExPASy Swiss-Prot
Match: Q9UDY4 (DnaJ homolog subfamily B member 4 OS=Homo sapiens OX=9606 GN=DNAJB4 PE=1 SV=1)

HSP 1 Score: 213.8 bits (543), Expect = 2.7e-54
Identity = 129/298 (43.29%), Postives = 181/298 (60.74%), Query Frame = 0


Query: 11  YNQISFIIQVLSDPQKKAIYDQYGEEGLKDMPPPGSASFPFGNGGGGGSSGFN-PRNAED 70
           + +++   +VLSDP+K+ IYDQ+GEEGLK     G A    G  G GG+  +    +   
Sbjct: 45  FKEVAEAYEVLSDPKKREIYDQFGEEGLK-----GGAG---GTDGQGGTFRYTFHGDPHA 104

Query: 71  IFAEFFGSSP-----FGFGSSGPGKSMRYQSEGGVFGGFGGSENIFRTYSENITP---KK 130
            FA FFG S      FG    G   S   + +G  F  FG S N +     ++ P   K+
Sbjct: 105 TFAAFFGGSNPFEIFFGRRMGGGRDSEEMEIDGDPFSAFGFSMNGYPRDRNSVGPSRLKQ 164

Query: 131 PPPVESKLPCTLEELYSGSTRKMKISRTVVDANGRQV-PETEILTIDVKPGWKKGTKITF 190
            PPV  +L  +LEE+YSG T++MKISR  ++A+GR    E +ILTI++K GWK+GTKITF
Sbjct: 165 DPPVIHELRVSLEEIYSGCTKRMKISRKRLNADGRSYRSEDKILTIEIKKGWKEGTKITF 224

Query: 191 PDKGNEQPNQLPADLVFIIDEKPHDVFKRDGNDIIMNHRVTLAEALGGTTINLTTLDGRN 250
           P +G+E PN +PAD+VFII +K H  FKRDG++II   +++L EAL G +IN+ TLDGRN
Sbjct: 225 PREGDETPNSIPADIVFIIKDKDHPKFKRDGSNIIYTAKISLREALCGCSINVPTLDGRN 284

Query: 251 LSIPVIDIVSPGYELVISKEGMPIVREPGNRGDLKIKFDVKFPTRLTPEQRAGLKRAL 299
           + + V DIV PG    I   G+P  + P  RGDL I+F+V FP  ++   +  L++ L
Sbjct: 285 IPMSVNDIVKPGMRRRIIGYGLPFPKNPDQRGDLLIEFEVSFPDTISSSSKEVLRKHL 334

[Close]

BLAST of Lsi04G021570 vs. ExPASy Swiss-Prot
Match: Q3MI00 (DnaJ homolog subfamily B member 1 OS=Bos taurus OX=9913 GN=DNAJB1 PE=2 SV=3)

HSP 1 Score: 213.0 bits (541), Expect = 4.6e-54
Identity = 126/300 (42.00%), Postives = 181/300 (60.33%), Query Frame = 0


Query: 11  YNQISFIIQVLSDPQKKAIYDQYGEEGLKDMPPPGSASFPFGNGGGGGSSGFNPRNAED- 70
           + +I+    VLSDP+K+ I+D+YGEEGLK   P G      G+ GG   + F+     D 
Sbjct: 45  FKEIAEAYDVLSDPRKREIFDRYGEEGLKGSGPSG------GSSGGTNGTSFSYTFHGDP 104

Query: 71  --IFAEFFGS-SPFG--FGSSGPGKSMRYQSE-GGVFGGFGGSENI----FRTYSENITP 130
             +FAEFFG  +PF   FG     + M       G   G GG  N+     R   E    
Sbjct: 105 HAMFAEFFGGRNPFDNFFGQRNGEEGMDIDDPFSGFPMGMGGFTNMNFGRSRPAQEPTRK 164

Query: 131 KKPPPVESKLPCTLEELYSGSTRKMKISRTVVDANGRQV-PETEILTIDVKPGWKKGTKI 190
           K+ PPV   L  +LEE+YSG T+KMKIS   ++ +G+ +  E +ILTI+VK GWK+GTKI
Sbjct: 165 KQDPPVTHDLRVSLEEIYSGCTKKMKISHKRLNPDGKSIRNEDKILTIEVKRGWKEGTKI 224

Query: 191 TFPDKGNEQPNQLPADLVFIIDEKPHDVFKRDGNDIIMNHRVTLAEALGGTTINLTTLDG 250
           TFP +G++  N +PAD+VF++ +KPH++FKRDG+D+I   R++L EAL G T+N+ TLDG
Sbjct: 225 TFPKEGDQTSNNIPADIVFVLKDKPHNIFKRDGSDVIYPARISLREALCGCTVNVPTLDG 284

Query: 251 RNLSIPVIDIVSPGYELVISKEGMPIVREPGNRGDLKIKFDVKFPTRLTPEQRAGLKRAL 299
           R + +   D++ PG    +  EG+P+ + P  RGDL I+F+V FP R+    R  L++ L
Sbjct: 285 RTIPVVFKDVIRPGMRRKVPGEGLPLPKTPEKRGDLIIEFEVIFPERIPQTSRTVLEQVL 338

[Close]

BLAST of Lsi04G021570 vs. ExPASy TrEMBL
Match: A0A1S3CR26 (dnaJ homolog subfamily B member 4 OS=Cucumis melo OX=3656 GN=LOC103503797 PE=4 SV=1)

HSP 1 Score: 554.7 bits (1428), Expect = 2.4e-154
Identity = 273/290 (94.14%), Postives = 282/290 (97.24%), Query Frame = 0


Query: 11  YNQISFIIQVLSDPQKKAIYDQYGEEGLKDMPPPGSASFPFGNGGGGGSSGFNPRNAEDI 70
           + QIS   +VLSDPQKKAIYDQYGEEGLKDMPPPGS  FPF NGGGGGSSGFNPRNAEDI
Sbjct: 47  FKQISEAYEVLSDPQKKAIYDQYGEEGLKDMPPPGSGGFPFANGGGGGSSGFNPRNAEDI 106

Query: 71  FAEFFGSSPFGFGSSGPGKSMRYQSEGGVFGGFGGSENIFRTYSENITPKKPPPVESKLP 130
           FAEFFGSSPFGFGSSGPGKSMRYQS+ G+FGGFGGSENIFRTYSEN+TPKKPPPVESKLP
Sbjct: 107 FAEFFGSSPFGFGSSGPGKSMRYQSD-GIFGGFGGSENIFRTYSENVTPKKPPPVESKLP 166

Query: 131 CTLEELYSGSTRKMKISRTVVDANGRQVPETEILTIDVKPGWKKGTKITFPDKGNEQPNQ 190
           CTLEELYSGSTRKMKISRTVVDANGRQVPETEILTIDVKPGWKKGTKITFPDKGNEQPNQ
Sbjct: 167 CTLEELYSGSTRKMKISRTVVDANGRQVPETEILTIDVKPGWKKGTKITFPDKGNEQPNQ 226

Query: 191 LPADLVFIIDEKPHDVFKRDGNDIIMNHRVTLAEALGGTTINLTTLDGRNLSIPVIDIVS 250
           LPADLVF+IDEKPHDVFKRDGNDIIM+HRVTLAEALGGTTINLTTLDGRNLSIPVIDIVS
Sbjct: 227 LPADLVFVIDEKPHDVFKRDGNDIIMSHRVTLAEALGGTTINLTTLDGRNLSIPVIDIVS 286

Query: 251 PGYELVISKEGMPIVREPGNRGDLKIKFDVKFPTRLTPEQRAGLKRALGG 301
           PGYELVI++EGMPIVREPGNRGDLKIKFDVKFPTRLTPEQRAGLKRALGG
Sbjct: 287 PGYELVIAREGMPIVREPGNRGDLKIKFDVKFPTRLTPEQRAGLKRALGG 335

[Close]

BLAST of Lsi04G021570 vs. ExPASy TrEMBL
Match: A0A6J1C4S9 (dnaJ homolog subfamily B member 4 OS=Momordica charantia OX=3673 GN=LOC111007916 PE=4 SV=1)

HSP 1 Score: 554.3 bits (1427), Expect = 3.1e-154
Identity = 268/290 (92.41%), Postives = 282/290 (97.24%), Query Frame = 0


Query: 11  YNQISFIIQVLSDPQKKAIYDQYGEEGLKDMPPPGSASFPFGNGGGGGSSGFNPRNAEDI 70
           + QIS   +VLSDPQKKAIYDQYGEEGLKDMPPPGS SFPFGNGG GGSSGFNPRNAEDI
Sbjct: 47  FKQISEAYEVLSDPQKKAIYDQYGEEGLKDMPPPGSGSFPFGNGGSGGSSGFNPRNAEDI 106

Query: 71  FAEFFGSSPFGFGSSGPGKSMRYQSEGGVFGGFGGSENIFRTYSENITPKKPPPVESKLP 130
           FAEFFGSSPFGFGSSGPGKSMRYQSEGG+FGGFGGSENIFRTYSE +TPKKPPPVE+KLP
Sbjct: 107 FAEFFGSSPFGFGSSGPGKSMRYQSEGGIFGGFGGSENIFRTYSEGVTPKKPPPVETKLP 166

Query: 131 CTLEELYSGSTRKMKISRTVVDANGRQVPETEILTIDVKPGWKKGTKITFPDKGNEQPNQ 190
           CTLEELYSGSTRKMKISRT+VDANGRQ+PETEILTIDVKPGWKKGTKITFPDKGNEQPNQ
Sbjct: 167 CTLEELYSGSTRKMKISRTIVDANGRQIPETEILTIDVKPGWKKGTKITFPDKGNEQPNQ 226

Query: 191 LPADLVFIIDEKPHDVFKRDGNDIIMNHRVTLAEALGGTTINLTTLDGRNLSIPVIDIVS 250
           LPADLVF+IDEKPHDVFKRDGNDIIMNHRV+LAEALGGTT++LTTLDGRNLSIPV DIVS
Sbjct: 227 LPADLVFVIDEKPHDVFKRDGNDIIMNHRVSLAEALGGTTVSLTTLDGRNLSIPVSDIVS 286

Query: 251 PGYELVISKEGMPIVREPGNRGDLKIKFDVKFPTRLTPEQRAGLKRALGG 301
           PGYELV++KEGMPIVREPGNRGDLKIKF+VKFPTRLTPEQRAGLKRALGG
Sbjct: 287 PGYELVVAKEGMPIVREPGNRGDLKIKFEVKFPTRLTPEQRAGLKRALGG 336

[Close]

BLAST of Lsi04G021570 vs. ExPASy TrEMBL
Match: A0A0A0LJC9 (J domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G878950 PE=4 SV=1)

HSP 1 Score: 553.9 bits (1426), Expect = 4.1e-154
Identity = 273/290 (94.14%), Postives = 282/290 (97.24%), Query Frame = 0


Query: 11  YNQISFIIQVLSDPQKKAIYDQYGEEGLKDMPPPGSASFPFGNGGGGGSSGFNPRNAEDI 70
           + QIS   +VLSDPQKKAIYDQYGEEGLKDMPPPGS  FPFGNGGGGGSSGFNPRNAEDI
Sbjct: 47  FKQISEAYEVLSDPQKKAIYDQYGEEGLKDMPPPGSGGFPFGNGGGGGSSGFNPRNAEDI 106

Query: 71  FAEFFGSSPFGFGSSGPGKSMRYQSEGGVFGGFGGSENIFRTYSENITPKKPPPVESKLP 130
           FAEFFGSSPFGFGSSGPGKSMRYQSE G+FGGFGGSENIFRTYSEN+TPKKP PVESKLP
Sbjct: 107 FAEFFGSSPFGFGSSGPGKSMRYQSE-GIFGGFGGSENIFRTYSENVTPKKPAPVESKLP 166

Query: 131 CTLEELYSGSTRKMKISRTVVDANGRQVPETEILTIDVKPGWKKGTKITFPDKGNEQPNQ 190
           CTLEELYSGSTRKMKISRTVVDANGRQVPETEILTIDVKPGWKKGTKITFPDKGNEQPNQ
Sbjct: 167 CTLEELYSGSTRKMKISRTVVDANGRQVPETEILTIDVKPGWKKGTKITFPDKGNEQPNQ 226

Query: 191 LPADLVFIIDEKPHDVFKRDGNDIIMNHRVTLAEALGGTTINLTTLDGRNLSIPVIDIVS 250
           LPADLVF+IDEKPHDVFKRDGNDIIMNHRVTLAEALGGTTINLTTLDGR+LSIPVIDIVS
Sbjct: 227 LPADLVFVIDEKPHDVFKRDGNDIIMNHRVTLAEALGGTTINLTTLDGRSLSIPVIDIVS 286

Query: 251 PGYELVISKEGMPIVREPGNRGDLKIKFDVKFPTRLTPEQRAGLKRALGG 301
           PGYELVI++EGMPIVREPGNRGDL+IKFDVKFPTRLTPEQRAGLKRALGG
Sbjct: 287 PGYELVIAREGMPIVREPGNRGDLRIKFDVKFPTRLTPEQRAGLKRALGG 335

[Close]

BLAST of Lsi04G021570 vs. ExPASy TrEMBL
Match: A0A6J1ILD2 (dnaJ homolog subfamily B member 4 OS=Cucurbita maxima OX=3661 GN=LOC111476642 PE=4 SV=1)

HSP 1 Score: 551.6 bits (1420), Expect = 2.0e-153
Identity = 269/290 (92.76%), Postives = 281/290 (96.90%), Query Frame = 0


Query: 11  YNQISFIIQVLSDPQKKAIYDQYGEEGLKDMPPPGSASFPFGNGGGGGSSGFNPRNAEDI 70
           + QIS   +VLSDPQKKAIYDQYGEEGLKD+PPP S SFPFGNG GGGSSGFNPRNAEDI
Sbjct: 47  FKQISEAYEVLSDPQKKAIYDQYGEEGLKDIPPPSSGSFPFGNGSGGGSSGFNPRNAEDI 106

Query: 71  FAEFFGSSPFGFGSSGPGKSMRYQSEGGVFGGFGGSENIFRTYSENITPKKPPPVESKLP 130
           FAEFFGSSPFGFGSSGPGKSMRYQSEGGVFGGFGGSEN+FRTYSEN+TPKKPPPVESKLP
Sbjct: 107 FAEFFGSSPFGFGSSGPGKSMRYQSEGGVFGGFGGSENLFRTYSENVTPKKPPPVESKLP 166

Query: 131 CTLEELYSGSTRKMKISRTVVDANGRQVPETEILTIDVKPGWKKGTKITFPDKGNEQPNQ 190
           CTLEELYSGSTRKMKISRTVVDANGR+VPETEILTIDVKPGWKKGTKITFPDKGNEQPNQ
Sbjct: 167 CTLEELYSGSTRKMKISRTVVDANGRRVPETEILTIDVKPGWKKGTKITFPDKGNEQPNQ 226

Query: 191 LPADLVFIIDEKPHDVFKRDGNDIIMNHRVTLAEALGGTTINLTTLDGRNLSIPVIDIVS 250
           LPADLVF+IDEKPHDVFKRDGNDIIMNH+VTLAEALGGTT+NLTTLDGRNLSIPVIDIVS
Sbjct: 227 LPADLVFVIDEKPHDVFKRDGNDIIMNHKVTLAEALGGTTVNLTTLDGRNLSIPVIDIVS 286

Query: 251 PGYELVISKEGMPIVREPGNRGDLKIKFDVKFPTRLTPEQRAGLKRALGG 301
           PGYEL I++EGMPIVREPGNRGDLKIKF+VKFPTRLT EQRAGLKRALGG
Sbjct: 287 PGYELTITREGMPIVREPGNRGDLKIKFEVKFPTRLTHEQRAGLKRALGG 336

[Close]

BLAST of Lsi04G021570 vs. ExPASy TrEMBL
Match: A0A5A7T6C0 (DnaJ-like protein subfamily B member 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold92G003450 PE=4 SV=1)

HSP 1 Score: 549.7 bits (1415), Expect = 7.7e-153
Identity = 270/281 (96.09%), Postives = 277/281 (98.58%), Query Frame = 0


Query: 20  VLSDPQKKAIYDQYGEEGLKDMPPPGSASFPFGNGGGGGSSGFNPRNAEDIFAEFFGSSP 79
           VLSDPQKKAIYDQYGEEGLKDMPPPGS  FPF NGGGGGSSGFNPRNAEDIFAEFFGSSP
Sbjct: 60  VLSDPQKKAIYDQYGEEGLKDMPPPGSGGFPFANGGGGGSSGFNPRNAEDIFAEFFGSSP 119

Query: 80  FGFGSSGPGKSMRYQSEGGVFGGFGGSENIFRTYSENITPKKPPPVESKLPCTLEELYSG 139
           FGFGSSGPGKSMRYQS+ G+FGGFGGSENIFRTYSEN+TPKKPPPVESKLPCTLEELYSG
Sbjct: 120 FGFGSSGPGKSMRYQSD-GIFGGFGGSENIFRTYSENVTPKKPPPVESKLPCTLEELYSG 179

Query: 140 STRKMKISRTVVDANGRQVPETEILTIDVKPGWKKGTKITFPDKGNEQPNQLPADLVFII 199
           STRKMKISRTVVDANGRQVPETEILTIDVKPGWKKGTKITFPDKGNEQPNQLPADLVF+I
Sbjct: 180 STRKMKISRTVVDANGRQVPETEILTIDVKPGWKKGTKITFPDKGNEQPNQLPADLVFVI 239

Query: 200 DEKPHDVFKRDGNDIIMNHRVTLAEALGGTTINLTTLDGRNLSIPVIDIVSPGYELVISK 259
           DEKPHDVFKRDGNDIIM+HRVTLAEALGGTTINLTTLDGRNLSIPVIDIVSPGYELVI++
Sbjct: 240 DEKPHDVFKRDGNDIIMSHRVTLAEALGGTTINLTTLDGRNLSIPVIDIVSPGYELVIAR 299

Query: 260 EGMPIVREPGNRGDLKIKFDVKFPTRLTPEQRAGLKRALGG 301
           EGMPIVREPGNRGDLKIKFDVKFPTRLTPEQRAGLKRALGG
Sbjct: 300 EGMPIVREPGNRGDLKIKFDVKFPTRLTPEQRAGLKRALGG 339

[Close]

BLAST of Lsi04G021570 vs. NCBI nr
Match: XP_008466370.1 (PREDICTED: dnaJ homolog subfamily B member 4 [Cucumis melo])

HSP 1 Score: 554.7 bits (1428), Expect = 5.0e-154
Identity = 273/290 (94.14%), Postives = 282/290 (97.24%), Query Frame = 0


Query: 11  YNQISFIIQVLSDPQKKAIYDQYGEEGLKDMPPPGSASFPFGNGGGGGSSGFNPRNAEDI 70
           + QIS   +VLSDPQKKAIYDQYGEEGLKDMPPPGS  FPF NGGGGGSSGFNPRNAEDI
Sbjct: 47  FKQISEAYEVLSDPQKKAIYDQYGEEGLKDMPPPGSGGFPFANGGGGGSSGFNPRNAEDI 106

Query: 71  FAEFFGSSPFGFGSSGPGKSMRYQSEGGVFGGFGGSENIFRTYSENITPKKPPPVESKLP 130
           FAEFFGSSPFGFGSSGPGKSMRYQS+ G+FGGFGGSENIFRTYSEN+TPKKPPPVESKLP
Sbjct: 107 FAEFFGSSPFGFGSSGPGKSMRYQSD-GIFGGFGGSENIFRTYSENVTPKKPPPVESKLP 166

Query: 131 CTLEELYSGSTRKMKISRTVVDANGRQVPETEILTIDVKPGWKKGTKITFPDKGNEQPNQ 190
           CTLEELYSGSTRKMKISRTVVDANGRQVPETEILTIDVKPGWKKGTKITFPDKGNEQPNQ
Sbjct: 167 CTLEELYSGSTRKMKISRTVVDANGRQVPETEILTIDVKPGWKKGTKITFPDKGNEQPNQ 226

Query: 191 LPADLVFIIDEKPHDVFKRDGNDIIMNHRVTLAEALGGTTINLTTLDGRNLSIPVIDIVS 250
           LPADLVF+IDEKPHDVFKRDGNDIIM+HRVTLAEALGGTTINLTTLDGRNLSIPVIDIVS
Sbjct: 227 LPADLVFVIDEKPHDVFKRDGNDIIMSHRVTLAEALGGTTINLTTLDGRNLSIPVIDIVS 286

Query: 251 PGYELVISKEGMPIVREPGNRGDLKIKFDVKFPTRLTPEQRAGLKRALGG 301
           PGYELVI++EGMPIVREPGNRGDLKIKFDVKFPTRLTPEQRAGLKRALGG
Sbjct: 287 PGYELVIAREGMPIVREPGNRGDLKIKFDVKFPTRLTPEQRAGLKRALGG 335

[Close]

BLAST of Lsi04G021570 vs. NCBI nr
Match: XP_038898720.1 (dnaJ homolog subfamily B member 1 [Benincasa hispida])

HSP 1 Score: 554.3 bits (1427), Expect = 6.5e-154
Identity = 276/291 (94.85%), Postives = 284/291 (97.59%), Query Frame = 0


Query: 11  YNQISFIIQVLSDPQKKAIYDQYGEEGLKDMPPPGSASFPFGN-GGGGGSSGFNPRNAED 70
           + QIS   +VLSDPQKKAIYDQYGEEGLKDMPPPGS SFPFGN GGGGGSSGFNPRNAED
Sbjct: 47  FKQISEAYEVLSDPQKKAIYDQYGEEGLKDMPPPGSGSFPFGNGGGGGGSSGFNPRNAED 106

Query: 71  IFAEFFGSSPFGFGSSGPGKSMRYQSEGGVFGGFGGSENIFRTYSENITPKKPPPVESKL 130
           IFAEFFGSSPFGFGSSGPGKSMRYQSE GVFGGFGGSENIFRTYSE++TPKKPPPVESKL
Sbjct: 107 IFAEFFGSSPFGFGSSGPGKSMRYQSE-GVFGGFGGSENIFRTYSESVTPKKPPPVESKL 166

Query: 131 PCTLEELYSGSTRKMKISRTVVDANGRQVPETEILTIDVKPGWKKGTKITFPDKGNEQPN 190
           PCTLEELYSGSTRKMKISRTVVDANGRQ+PETEILTIDVKPGWKKGTKITFPDKGNEQPN
Sbjct: 167 PCTLEELYSGSTRKMKISRTVVDANGRQLPETEILTIDVKPGWKKGTKITFPDKGNEQPN 226

Query: 191 QLPADLVFIIDEKPHDVFKRDGNDIIMNHRVTLAEALGGTTINLTTLDGRNLSIPVIDIV 250
           QLPADLVF+IDEKPHDVFKRDGNDIIMNHRVTLAEALGGTTINLTTLDGRNLSIPVIDIV
Sbjct: 227 QLPADLVFVIDEKPHDVFKRDGNDIIMNHRVTLAEALGGTTINLTTLDGRNLSIPVIDIV 286

Query: 251 SPGYELVISKEGMPIVREPGNRGDLKIKFDVKFPTRLTPEQRAGLKRALGG 301
           SPGYELVI++EGMPIVREPGNRGDLKIKFDVKFPTRLTPEQRAGLKRALGG
Sbjct: 287 SPGYELVIAREGMPIVREPGNRGDLKIKFDVKFPTRLTPEQRAGLKRALGG 336

[Close]

BLAST of Lsi04G021570 vs. NCBI nr
Match: XP_022136157.1 (dnaJ homolog subfamily B member 4 [Momordica charantia])

HSP 1 Score: 554.3 bits (1427), Expect = 6.5e-154
Identity = 268/290 (92.41%), Postives = 282/290 (97.24%), Query Frame = 0


Query: 11  YNQISFIIQVLSDPQKKAIYDQYGEEGLKDMPPPGSASFPFGNGGGGGSSGFNPRNAEDI 70
           + QIS   +VLSDPQKKAIYDQYGEEGLKDMPPPGS SFPFGNGG GGSSGFNPRNAEDI
Sbjct: 47  FKQISEAYEVLSDPQKKAIYDQYGEEGLKDMPPPGSGSFPFGNGGSGGSSGFNPRNAEDI 106

Query: 71  FAEFFGSSPFGFGSSGPGKSMRYQSEGGVFGGFGGSENIFRTYSENITPKKPPPVESKLP 130
           FAEFFGSSPFGFGSSGPGKSMRYQSEGG+FGGFGGSENIFRTYSE +TPKKPPPVE+KLP
Sbjct: 107 FAEFFGSSPFGFGSSGPGKSMRYQSEGGIFGGFGGSENIFRTYSEGVTPKKPPPVETKLP 166

Query: 131 CTLEELYSGSTRKMKISRTVVDANGRQVPETEILTIDVKPGWKKGTKITFPDKGNEQPNQ 190
           CTLEELYSGSTRKMKISRT+VDANGRQ+PETEILTIDVKPGWKKGTKITFPDKGNEQPNQ
Sbjct: 167 CTLEELYSGSTRKMKISRTIVDANGRQIPETEILTIDVKPGWKKGTKITFPDKGNEQPNQ 226

Query: 191 LPADLVFIIDEKPHDVFKRDGNDIIMNHRVTLAEALGGTTINLTTLDGRNLSIPVIDIVS 250
           LPADLVF+IDEKPHDVFKRDGNDIIMNHRV+LAEALGGTT++LTTLDGRNLSIPV DIVS
Sbjct: 227 LPADLVFVIDEKPHDVFKRDGNDIIMNHRVSLAEALGGTTVSLTTLDGRNLSIPVSDIVS 286

Query: 251 PGYELVISKEGMPIVREPGNRGDLKIKFDVKFPTRLTPEQRAGLKRALGG 301
           PGYELV++KEGMPIVREPGNRGDLKIKF+VKFPTRLTPEQRAGLKRALGG
Sbjct: 287 PGYELVVAKEGMPIVREPGNRGDLKIKFEVKFPTRLTPEQRAGLKRALGG 336

[Close]

BLAST of Lsi04G021570 vs. NCBI nr
Match: XP_004136336.1 (dnaJ homolog subfamily B member 1 [Cucumis sativus] >KGN60126.1 hypothetical protein Csa_001601 [Cucumis sativus])

HSP 1 Score: 553.9 bits (1426), Expect = 8.5e-154
Identity = 273/290 (94.14%), Postives = 282/290 (97.24%), Query Frame = 0


Query: 11  YNQISFIIQVLSDPQKKAIYDQYGEEGLKDMPPPGSASFPFGNGGGGGSSGFNPRNAEDI 70
           + QIS   +VLSDPQKKAIYDQYGEEGLKDMPPPGS  FPFGNGGGGGSSGFNPRNAEDI
Sbjct: 47  FKQISEAYEVLSDPQKKAIYDQYGEEGLKDMPPPGSGGFPFGNGGGGGSSGFNPRNAEDI 106

Query: 71  FAEFFGSSPFGFGSSGPGKSMRYQSEGGVFGGFGGSENIFRTYSENITPKKPPPVESKLP 130
           FAEFFGSSPFGFGSSGPGKSMRYQSE G+FGGFGGSENIFRTYSEN+TPKKP PVESKLP
Sbjct: 107 FAEFFGSSPFGFGSSGPGKSMRYQSE-GIFGGFGGSENIFRTYSENVTPKKPAPVESKLP 166

Query: 131 CTLEELYSGSTRKMKISRTVVDANGRQVPETEILTIDVKPGWKKGTKITFPDKGNEQPNQ 190
           CTLEELYSGSTRKMKISRTVVDANGRQVPETEILTIDVKPGWKKGTKITFPDKGNEQPNQ
Sbjct: 167 CTLEELYSGSTRKMKISRTVVDANGRQVPETEILTIDVKPGWKKGTKITFPDKGNEQPNQ 226

Query: 191 LPADLVFIIDEKPHDVFKRDGNDIIMNHRVTLAEALGGTTINLTTLDGRNLSIPVIDIVS 250
           LPADLVF+IDEKPHDVFKRDGNDIIMNHRVTLAEALGGTTINLTTLDGR+LSIPVIDIVS
Sbjct: 227 LPADLVFVIDEKPHDVFKRDGNDIIMNHRVTLAEALGGTTINLTTLDGRSLSIPVIDIVS 286

Query: 251 PGYELVISKEGMPIVREPGNRGDLKIKFDVKFPTRLTPEQRAGLKRALGG 301
           PGYELVI++EGMPIVREPGNRGDL+IKFDVKFPTRLTPEQRAGLKRALGG
Sbjct: 287 PGYELVIAREGMPIVREPGNRGDLRIKFDVKFPTRLTPEQRAGLKRALGG 335

[Close]

BLAST of Lsi04G021570 vs. NCBI nr
Match: XP_023535593.1 (dnaJ homolog subfamily B member 4-like [Cucurbita pepo subsp. pepo] >KAG6591589.1 DnaJ-like subfamily B member 4, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 551.6 bits (1420), Expect = 4.2e-153
Identity = 269/290 (92.76%), Postives = 281/290 (96.90%), Query Frame = 0


Query: 11  YNQISFIIQVLSDPQKKAIYDQYGEEGLKDMPPPGSASFPFGNGGGGGSSGFNPRNAEDI 70
           + QIS   +VLSDPQKKAIYDQYGEEGLKD+PPP S SFPFGNG GGGSSGFNPRNAEDI
Sbjct: 47  FKQISEAYEVLSDPQKKAIYDQYGEEGLKDIPPPSSGSFPFGNGSGGGSSGFNPRNAEDI 106

Query: 71  FAEFFGSSPFGFGSSGPGKSMRYQSEGGVFGGFGGSENIFRTYSENITPKKPPPVESKLP 130
           FAEFFGSSPFGFGSSGPGKSMRYQSEGGVFGGFGGSEN+FRTYSEN+TPKKPPPVESKLP
Sbjct: 107 FAEFFGSSPFGFGSSGPGKSMRYQSEGGVFGGFGGSENLFRTYSENVTPKKPPPVESKLP 166

Query: 131 CTLEELYSGSTRKMKISRTVVDANGRQVPETEILTIDVKPGWKKGTKITFPDKGNEQPNQ 190
           CTLEELYSGSTRKMKISRTVVDANGR+VPETEILTIDVKPGWKKGTKITFPDKGNEQPNQ
Sbjct: 167 CTLEELYSGSTRKMKISRTVVDANGRRVPETEILTIDVKPGWKKGTKITFPDKGNEQPNQ 226

Query: 191 LPADLVFIIDEKPHDVFKRDGNDIIMNHRVTLAEALGGTTINLTTLDGRNLSIPVIDIVS 250
           LPADLVF+IDEKPHDVFKRDGNDIIMNH+VTLAEALGGTT+NLTTLDGRNLSIPVIDIVS
Sbjct: 227 LPADLVFVIDEKPHDVFKRDGNDIIMNHKVTLAEALGGTTVNLTTLDGRNLSIPVIDIVS 286

Query: 251 PGYELVISKEGMPIVREPGNRGDLKIKFDVKFPTRLTPEQRAGLKRALGG 301
           PGYEL I++EGMPIVREPGNRGDLKIKF+VKFPTRLT EQRAGLKRALGG
Sbjct: 287 PGYELAITREGMPIVREPGNRGDLKIKFEVKFPTRLTHEQRAGLKRALGG 336

[Close]

BLAST of Lsi04G021570 vs. TAIR 10
Match: AT5G25530.1 (DNAJ heat shock family protein )

HSP 1 Score: 428.3 bits (1100), Expect = 5.0e-120
Identity = 217/293 (74.06%), Postives = 244/293 (83.28%), Query Frame = 0


Query: 17  IIQVLSDPQKKAIYDQYGEEGLKDMPPPGSASFPFGNGGGGGSSGFNPRNAEDIFAEFFG 76
           + QVLSDPQK+A+YDQYGEEGL DMPPPGS       G  G + GFNPRNAEDIFAEFFG
Sbjct: 61  MFQVLSDPQKRAVYDQYGEEGLSDMPPPGS------TGNNGRAGGFNPRNAEDIFAEFFG 120

Query: 77  SSPFGFGSS-GPGKSMRYQSE--GGVFGGFGG-----SENIFRTYSENI-TPKKPPPVES 136
           SSPFGFGS+ GPG+SMR+QS+  GG+FGGFGG       NIFRTYSE    PKKPPPVES
Sbjct: 121 SSPFGFGSAGGPGRSMRFQSDGGGGMFGGFGGGNNGSENNIFRTYSEGTPAPKKPPPVES 180

Query: 137 KLPCTLEELYSGSTRKMKISRTVVDANGRQVPETEILTIDVKPGWKKGTKITFPDKGNEQ 196
           KLPC+LEELYSGSTRKMKISR++VDANGRQ  ETEILTI VKPGWKKGTKI FPDKGNEQ
Sbjct: 181 KLPCSLEELYSGSTRKMKISRSIVDANGRQAQETEILTIVVKPGWKKGTKIKFPDKGNEQ 240

Query: 197 PNQLPADLVFIIDEKPHDVFKRDGNDIIMNHRVTLAEALGGTTINLTTLDGRNLSIPVID 256
            NQLPADLVF+IDEKPHD+F RDGND+I + RVTLAEA+GGTT+N+ TLDGRNL + V +
Sbjct: 241 VNQLPADLVFVIDEKPHDLFTRDGNDLITSRRVTLAEAIGGTTVNINTLDGRNLPVGVAE 300

Query: 257 IVSPGYELVISKEGMPIVREPGNRGDLKIKFDVKFPTRLTPEQRAGLKRALGG 301
           IVSPGYE V+  EGMPI +EP N+GDLKIKFDV+FP RLT EQ++ LKR L G
Sbjct: 301 IVSPGYEFVVPGEGMPIAKEPRNKGDLKIKFDVQFPARLTTEQKSALKRVLAG 347

[Close]

BLAST of Lsi04G021570 vs. TAIR 10
Match: AT2G20560.1 (DNAJ heat shock family protein )

HSP 1 Score: 322.4 bits (825), Expect = 3.9e-88
Identity = 175/301 (58.14%), Postives = 222/301 (73.75%), Query Frame = 0


Query: 10  LYNQISFIIQVLSDPQKKAIYDQYGEEGLK-DMPPP--GSASFPFGNGGGGGSSGFNPRN 69
           ++ QIS   +VLSDPQKKA+YDQYGEEGLK ++PPP  G A++ F  G G  S  FNPRN
Sbjct: 46  MFKQISEAYEVLSDPQKKAVYDQYGEEGLKGNVPPPDAGGATY-FSTGDGPTSFRFNPRN 105

Query: 70  AEDIFAEFFG-SSPFG-------FGSSGPGKSMRYQSEGGVFGGFGGSENIFRTYSENIT 129
           A+DIFAEFFG SSPFG       F SS  G +M + S G   GG GGS         +  
Sbjct: 106 ADDIFAEFFGFSSPFGGGRGGTRFSSSMFGDNM-FASFGEGGGGGGGS-------MHHGG 165

Query: 130 PKKPPPVESKLPCTLEELYSGSTRKMKISRTVVDANGRQVPETEILTIDVKPGWKKGTKI 189
            +K  P+E+KLPC+LE+LY G+T+KM+ISR + D +G+ +   EILTIDVKPGWKKGTKI
Sbjct: 166 ARKAAPIENKLPCSLEDLYKGTTKKMRISREIADVSGKTMQVEEILTIDVKPGWKKGTKI 225

Query: 190 TFPDKGNEQPNQLPADLVFIIDEKPHDVFKRDGNDIIMNHRVTLAEALGGTTINLTTLDG 249
           TFP+KGNEQP  +PADLVFIIDEKPH VF R+GND+I+  +++L EAL G T+NLTTLDG
Sbjct: 226 TFPEKGNEQPGVIPADLVFIIDEKPHPVFTREGNDLIVTQKISLVEALTGYTVNLTTLDG 285

Query: 250 RNLSIPVIDIVSPGYELVISKEGMPIVREPGNRGDLKIKFDVKFPTRLTPEQRAGLKRAL 300
           R L+IPV ++V P YE V+ KEGMP+ ++   RG+L+IKF++KFPTRLT EQ+ G+K+ L
Sbjct: 286 RRLTIPVTNVVHPEYEEVVPKEGMPLQKDQTKRGNLRIKFNIKFPTRLTSEQKTGVKKLL 337

[Close]

BLAST of Lsi04G021570 vs. TAIR 10
Match: AT4G28480.1 (DNAJ heat shock family protein )

HSP 1 Score: 313.9 bits (803), Expect = 1.4e-85
Identity = 164/303 (54.13%), Postives = 215/303 (70.96%), Query Frame = 0


Query: 11  YNQISFIIQVLSDPQKKAIYDQYGEEGLK-DMPPPGSASFP---FGNGGGGGSSGFNPRN 70
           + QIS    VLSDPQK+A+YDQYGEEGLK ++PPP +A+     F  G G  S  FNPR+
Sbjct: 47  FKQISEAYDVLSDPQKRAVYDQYGEEGLKGNVPPPNAATSGASYFSTGDGSSSFRFNPRS 106

Query: 71  AEDIFAEFFG-SSPFGFGSSGPGKSM---------RYQSEGGVFGGFGGSENIFRTYSEN 130
           A+DIFAEFFG S+PFG G  G G             Y S G   GG GG+ +    +  +
Sbjct: 107 ADDIFAEFFGFSTPFGGGGGGTGGQRFASRMFGDDMYASFGEGAGG-GGAMHHHHHHHHH 166

Query: 131 ITPKKPPPVESKLPCTLEELYSGSTRKMKISRTVVDANGRQVPETEILTIDVKPGWKKGT 190
              +K  P+E+KLPC+LE+LY G+T+KMKISR +VD +G+ +   EILTI VKPGWKKGT
Sbjct: 167 AAARKVAPIENKLPCSLEDLYKGTTKKMKISREIVDVSGKAMQVEEILTIGVKPGWKKGT 226

Query: 191 KITFPDKGNEQPNQLPADLVFIIDEKPHDVFKRDGNDIIMNHRVTLAEALGGTTINLTTL 250
           KITFP+KGNE P  +PADLVFIIDEKPH VF R+GND+I+  +V+LA+AL G T N+ TL
Sbjct: 227 KITFPEKGNEHPGVIPADLVFIIDEKPHPVFTREGNDLIVTQKVSLADALTGYTANIATL 286

Query: 251 DGRNLSIPVIDIVSPGYELVISKEGMPIVREPGNRGDLKIKFDVKFPTRLTPEQRAGLKR 300
           DGR L+IP+ +++ P YE V+ KEGMP+ ++   +G+L+IKF++KFP RLT EQ+AG K+
Sbjct: 287 DGRTLTIPITNVIHPEYEEVVPKEGMPLQKDQTKKGNLRIKFNIKFPARLTAEQKAGFKK 346

[Close]

BLAST of Lsi04G021570 vs. TAIR 10
Match: AT3G08910.1 (DNAJ heat shock family protein )

HSP 1 Score: 300.4 bits (768), Expect = 1.6e-81
Identity = 157/288 (54.51%), Postives = 208/288 (72.22%), Query Frame = 0


Query: 11  YNQISFIIQVLSDPQKKAIYDQYGEEGL-KDMPPPGSASFPFGNGGGGGSSGFNPRNAED 70
           + QIS    VLSDPQK+AIYDQYGEEGL    PPPG+     G   GG S  FN R+A+D
Sbjct: 47  FKQISEAYDVLSDPQKRAIYDQYGEEGLTSQAPPPGAGG---GFSDGGASFRFNGRSADD 106

Query: 71  IFAEFFG-SSPFGFGSSGPGKSMRYQSEGGVFGGFGGSENIFRTYSENITPKKPPPVESK 130
           IF+EFFG + PFG  S G G S           GF  +E++F   S  + P+K  P+E +
Sbjct: 107 IFSEFFGFTRPFG-DSRGAGPS----------NGFRFAEDVFS--SNVVPPRKAAPIERQ 166

Query: 131 LPCTLEELYSGSTRKMKISRTVVDANGRQVPETEILTIDVKPGWKKGTKITFPDKGNEQP 190
           LPC+LE+LY G ++KMKISR V+D++GR     EILTI++KPGWKKGTKITFP+KGNEQ 
Sbjct: 167 LPCSLEDLYKGVSKKMKISRDVLDSSGRPTTVEEILTIEIKPGWKKGTKITFPEKGNEQR 226

Query: 191 NQLPADLVFIIDEKPHDVFKRDGNDIIMNHRVTLAEALGGTTINLTTLDGRNLSIPVIDI 250
             +P+DLVFI+DEKPH VFKRDGND++M  ++ L EAL G T  ++TLDGR++++P+ ++
Sbjct: 227 GIIPSDLVFIVDEKPHAVFKRDGNDLVMTQKIPLVEALTGYTAQVSTLDGRSVTVPINNV 286

Query: 251 VSPGYELVISKEGMPIVREPGNRGDLKIKFDVKFPTRLTPEQRAGLKR 297
           +SP YE V+  EGMPI ++P  +G+L+IKF VKFP+RLT EQ++G+KR
Sbjct: 287 ISPSYEEVVKGEGMPIPKDPSKKGNLRIKFTVKFPSRLTTEQKSGIKR 318

[Close]

BLAST of Lsi04G021570 vs. TAIR 10
Match: AT1G59725.1 (DNAJ heat shock family protein )

HSP 1 Score: 296.2 bits (757), Expect = 3.0e-80
Identity = 162/298 (54.36%), Postives = 201/298 (67.45%), Query Frame = 0


Query: 11  YNQISFIIQVLSDPQKKAIYDQYGEEGLKDMPPPGSASFPFGNGGGGGSSGFN------P 70
           + QIS    VLSDP K+ IYDQYGE+GL       +AS    N   G ++ +N      P
Sbjct: 48  FKQISEAYDVLSDPNKRQIYDQYGEDGL--TATEATASSQQHNYSSGNNNNYNAGFRYYP 107

Query: 71  RNAEDIFAEFFGSSPFGFGSSGPGKSMRYQSEGGVFGG--FGGSENIFRTYSENITPKKP 130
           R+AEDIFAEFFG+S   F             +GGV GG  F  +E   +T  +    +K 
Sbjct: 108 RDAEDIFAEFFGASEKVF-------------DGGVGGGGRFKSAEAGSQTNRKTPVNRKA 167

Query: 131 PPVESKLPCTLEELYSGSTRKMKISRTVVDANGRQVPETEILTIDVKPGWKKGTKITFPD 190
           P +ESKL CTLEELY G  RKMKISR V D  G+  P  EIL ID+ PGWKKGTKITFP+
Sbjct: 168 PAIESKLACTLEELYKGGRRKMKISRVVPDGLGKSKPVEEILKIDITPGWKKGTKITFPE 227

Query: 191 KGNEQPNQLPADLVFIIDEKPHDVFKRDGNDIIMNHRVTLAEALGGTTINLTTLDGRNLS 250
           KGN++P   PADL+F+IDEKPH V+KRDGND+I++ +V+L EAL G T++LTTLDGRNL+
Sbjct: 228 KGNQEPGVTPADLIFVIDEKPHSVYKRDGNDLIVDKKVSLLEALTGITLSLTTLDGRNLT 287

Query: 251 IPVIDIVSPGYELVISKEGMPIVREPGNRGDLKIKFDVKFPTRLTPEQRAGLKRALGG 301
           IPV+DIV PG E+VI  EGMPI +E   RGDL+I F++ FP+RLT EQ+  LKR LGG
Sbjct: 288 IPVLDIVKPGQEIVIPSEGMPISKEGSKRGDLRINFEICFPSRLTSEQKTDLKRVLGG 330

The following BLAST results are available for this feature:

BLAST of Lsi04G021570 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P25685	1.4e-55	42.33	DnaJ homolog subfamily B member 1 OS=Homo sapiens OX=9606 GN=DNAJB1 PE=1 SV=4	[more]
Q5R8J8	2.1e-54	43.29	DnaJ homolog subfamily B member 4 OS=Pongo abelii OX=9601 GN=DNAJB4 PE=2 SV=1	[more]
Q9QYJ3	2.7e-54	42.00	DnaJ homolog subfamily B member 1 OS=Mus musculus OX=10090 GN=Dnajb1 PE=1 SV=3	[more]
Q9UDY4	2.7e-54	43.29	DnaJ homolog subfamily B member 4 OS=Homo sapiens OX=9606 GN=DNAJB4 PE=1 SV=1	[more]
Q3MI00	4.6e-54	42.00	DnaJ homolog subfamily B member 1 OS=Bos taurus OX=9913 GN=DNAJB1 PE=2 SV=3	[more]

BLAST of Lsi04G021570 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S3CR26	2.4e-154	94.14	dnaJ homolog subfamily B member 4 OS=Cucumis melo OX=3656 GN=LOC103503797 PE=4 S...	[more]
A0A6J1C4S9	3.1e-154	92.41	dnaJ homolog subfamily B member 4 OS=Momordica charantia OX=3673 GN=LOC111007916...	[more]
A0A0A0LJC9	4.1e-154	94.14	J domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G878950 PE=4 SV=1	[more]
A0A6J1ILD2	2.0e-153	92.76	dnaJ homolog subfamily B member 4 OS=Cucurbita maxima OX=3661 GN=LOC111476642 PE...	[more]
A0A5A7T6C0	7.7e-153	96.09	DnaJ-like protein subfamily B member 4 OS=Cucumis melo var. makuwa OX=1194695 GN...	[more]

BLAST of Lsi04G021570 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_008466370.1	5.0e-154	94.14	PREDICTED: dnaJ homolog subfamily B member 4 [Cucumis melo]	[more]
XP_038898720.1	6.5e-154	94.85	dnaJ homolog subfamily B member 1 [Benincasa hispida]	[more]
XP_022136157.1	6.5e-154	92.41	dnaJ homolog subfamily B member 4 [Momordica charantia]	[more]
XP_004136336.1	8.5e-154	94.14	dnaJ homolog subfamily B member 1 [Cucumis sativus] >KGN60126.1 hypothetical pro...	[more]
XP_023535593.1	4.2e-153	92.76	dnaJ homolog subfamily B member 4-like [Cucurbita pepo subsp. pepo] >KAG6591589....	[more]

BLAST of Lsi04G021570 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G25530.1	5.0e-120	74.06	DNAJ heat shock family protein	[more]
AT2G20560.1	3.9e-88	58.14	DNAJ heat shock family protein	[more]
AT4G28480.1	1.4e-85	54.13	DNAJ heat shock family protein	[more]
AT3G08910.1	1.6e-81	54.51	DNAJ heat shock family protein	[more]
AT1G59725.1	3.0e-80	54.36	DNAJ heat shock family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002939	Chaperone DnaJ, C-terminal	PFAM	PF01556	DnaJ_C	coord: 125..283 e-value: 6.2E-43 score: 146.6
None	No IPR available	GENE3D	2.60.260.20		coord: 203..299 e-value: 2.3E-27 score: 97.1
None	No IPR available	GENE3D	2.60.260.20		coord: 124..202 e-value: 1.1E-31 score: 110.7
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 39..63
None	No IPR available	PANTHER	PTHR24078	DNAJ HOMOLOG SUBFAMILY C MEMBER	coord: 11..300
None	No IPR available	PANTHER	PTHR24078:SF538	DNAJ HEAT SHOCK FAMILY PROTEIN	coord: 11..300
None	No IPR available	CDD	cd10747	DnaJ_C	coord: 123..286 e-value: 5.19853E-54 score: 171.454
IPR036869	Chaperone J-domain superfamily	GENE3D	1.10.287.110	DnaJ domain	coord: 10..85 e-value: 1.3E-7 score: 34.1
IPR036869	Chaperone J-domain superfamily	SUPERFAMILY	46565	Chaperone J-domain	coord: 12..80
IPR008971	HSP40/DnaJ peptide-binding	SUPERFAMILY	49493	HSP40/DnaJ peptide-binding domain	coord: 206..292
IPR008971	HSP40/DnaJ peptide-binding	SUPERFAMILY	49493	HSP40/DnaJ peptide-binding domain	coord: 124..205

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi04G021570.1	Lsi04G021570.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0051085	chaperone cofactor-dependent protein refolding
biological_process	GO:0006457	protein folding
cellular_component	GO:0005829	cytosol
molecular_function	GO:0051087	chaperone binding
molecular_function	GO:0051082	unfolded protein binding