Lsi04G020700 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi04G020700
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	proton pump-interactor 1-like
Location	chr04: 27854544 .. 27855407 (-)
RNA-Seq Expression	Lsi04G020700
Synteny	Lsi04G020700

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGATAGTGACATGATTCTATATTTACCTCAGGACCGTGAAAAGAAAGCAAAGAAGAAGGCAACAGCTTCTGGATCTGCCGCATTTCCTGATGAAGAACCTGCAGATGCTGAAGCTGATGCAGTTGAGGAGGAATCTACAGAGCTTGAAAAGGTTAATGAAAGTAGTGAAGCTCCGGTTGTTCCAGTTAAGACTAAAACCCCAAAGGAGGCTGGCATTAGGAGGCCTGGCAGGCAAAGAGGGCTGGATTCTGTTCCGAAAGTGATTCGGAAGCGCAAGAAGTCGATCAATTACTTGGAATGGGCTGGTCCTGCTGCTGCTGTTGCTGTAGTGTTACTTGTGATCCTTCTGGTGCTTGGATATTACTACTACTACCTTCTCTAAAAACTGTGAAACTAGGAGTTAGAAGAGGTTTATGGATGGGACAAATTTTCTGTTGATGAGCATGGTGATGATGAGTGGTTTTAGAGAATCAGTTGTATTTTTAATTATGTTTTATTTCTGTTTATTTCATCCTTACAAGGGATGGACTCAACTCATAATGGTTGGCATTTTAGTCAGGTTCACAGTGTTTACTGAGGTTGAAACATGCATTTGGTTGGTTCTTTCAATGTATTTCCAAATTTTGACTTTGATCAATCTTCATTGCTGATATATCTTGAGGCCTCAAATTTGCCTTTTGTAATAGGATGAATTGGCTTGTTGCCATTTTGACTGATTTGCATGATTTGAAATTGTAAAGCTTAAAAGTTTTATGTGGACGGAATATCTGAACTTGATGTTATTTAAATTTTAGGGAGAAAATCTAAGTGTATGGGGGTTGAGTAATAACATAAAATTAACTCAATTTGCTTGTAGTGAATGA

mRNA sequence

ATGATAGACCGTGAAAAGAAAGCAAAGAAGAAGGCAACAGCTTCTGGATCTGCCGCATTTCCTGATGAAGAACCTGCAGATGCTGAAGCTGATGCAGTTGAGGAGGAATCTACAGAGCTTGAAAAGGTTAATGAAAGTAGTGAAGCTCCGGTTGTTCCAGTTAAGACTAAAACCCCAAAGGAGGCTGGCATTAGGAGGCCTGGCAGGCAAAGAGGGCTGGATTCTGTTCCGAAAGTGATTCGGAAGCGCAAGAAGTCGATCAATTACTTGGAATGGGCTGGTCCTGCTGCTGCTGTTGCTGTAGTGTTACTTGTGATCCTTCTGGGAGAAAATCTAAGTGTATGGGGGTTGAGTAATAACATAAAATTAACTCAATTTGCTTGTAGTGAATGA

Coding sequence (CDS)

ATGATAGACCGTGAAAAGAAAGCAAAGAAGAAGGCAACAGCTTCTGGATCTGCCGCATTTCCTGATGAAGAACCTGCAGATGCTGAAGCTGATGCAGTTGAGGAGGAATCTACAGAGCTTGAAAAGGTTAATGAAAGTAGTGAAGCTCCGGTTGTTCCAGTTAAGACTAAAACCCCAAAGGAGGCTGGCATTAGGAGGCCTGGCAGGCAAAGAGGGCTGGATTCTGTTCCGAAAGTGATTCGGAAGCGCAAGAAGTCGATCAATTACTTGGAATGGGCTGGTCCTGCTGCTGCTGTTGCTGTAGTGTTACTTGTGATCCTTCTGGGAGAAAATCTAAGTGTATGGGGGTTGAGTAATAACATAAAATTAACTCAATTTGCTTGTAGTGAATGA

Protein sequence

MIDREKKAKKKATASGSAAFPDEEPADAEADAVEEESTELEKVNESSEAPVVPVKTKTPKEAGIRRPGRQRGLDSVPKVIRKRKKSINYLEWAGPAAAVAVVLLVILLGENLSVWGLSNNIKLTQFACSE

Homology

[Close]

BLAST of Lsi04G020700 vs. ExPASy Swiss-Prot
Match: O23144 (Proton pump-interactor 1 OS=Arabidopsis thaliana OX=3702 GN=PPI1 PE=1 SV=2)

HSP 1 Score: 49.3 bits (116), Expect = 3.8e-05
Identity = 47/122 (38.52%), Postives = 67/122 (54.92%), Query Frame = 0


Query: 2   IDREKKAKKKATASGSAAFPDEEPADAEADAVEEE----------STELEKVNESS---- 61
           ++R+KK  +KA A   AA   ++ A+ +    +E+           TE E+V E+S    
Sbjct: 487 MERKKKLAEKAAA--KAAIRAQKEAEKKEKKEQEKKAKKKTGGNTETETEEVPEASEEEI 546

Query: 62  EAPV---VPVKTKTPKEAGIRRPGRQRGLDSVPKVIRKRKKSINYLEWAGPAAAVAVVLL 107
           EAPV    P K K  KE  IR   R RG +++P+ I KRKKS NY  +A PAA V ++LL
Sbjct: 547 EAPVQEEKPQKEKVFKEKPIRNRTRGRGPETIPRAILKRKKSTNYWVYAAPAALVVLLLL 606

[Close]

BLAST of Lsi04G020700 vs. ExPASy Swiss-Prot
Match: B3H4K7 (Proton pump-interactor 2 OS=Arabidopsis thaliana OX=3702 GN=PPI2 PE=2 SV=1)

HSP 1 Score: 44.7 bits (104), Expect = 9.5e-04
Identity = 37/107 (34.58%), Postives = 58/107 (54.21%), Query Frame = 0


Query: 5   EKKAKKKATASGSAAFPDEEPADAEADAVEEESTELEKVNE---SSEAPVVPVKTKTPKE 64
           EKKAKKKA A+ S        + +E+D  +E + +LEKV     S +      ++  PK+
Sbjct: 488 EKKAKKKAAANSS--------SPSESDHSQEVTKDLEKVRTLAVSGKEKHQKERSLFPKQ 547

Query: 65  AGIRRPGRQRGLDSVPKVIRKRKKSINYLEWAGPAAAVAVVLLVILL 109
              R   R RG +++PK I  R+K+  Y  W   +AA+AV L +++L
Sbjct: 548 RSFRYKHRGRGTEALPKAILNRRKAHKYWVWGLSSAALAVALFLVVL 586

[Close]

BLAST of Lsi04G020700 vs. ExPASy TrEMBL
Match: A0A0A0L261 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G637150 PE=4 SV=1)

HSP 1 Score: 152.5 bits (384), Expect = 1.2e-33
Identity = 91/108 (84.26%), Postives = 95/108 (87.96%), Query Frame = 0


Query: 2   IDREKKAKKKATASGS-AAFPDEEPADAEADAVEEESTELEKVNESSEAPVVPVKTKTPK 61
           IDREKKAKKKAT SGS A  PDEEP D  ADAVEEESTE+EKVNE++EAP VPVKTK PK
Sbjct: 525 IDREKKAKKKATTSGSFATLPDEEPTD--ADAVEEESTEVEKVNETAEAP-VPVKTKIPK 584

Query: 62  EAGIRRPGRQRGLDSVPKVIRKRKKSINYLEWAGPAAAVAVVLLVILL 109
           E  +RRPGRQRGLDSVPKVIRKRKKSINYLEWAGPAAA A VLLVILL
Sbjct: 585 ETVVRRPGRQRGLDSVPKVIRKRKKSINYLEWAGPAAAGAAVLLVILL 629

[Close]

BLAST of Lsi04G020700 vs. ExPASy TrEMBL
Match: A0A5D3D9V7 (Proton pump-interactor 1 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold134G004000 PE=4 SV=1)

HSP 1 Score: 151.4 bits (381), Expect = 2.7e-33
Identity = 90/108 (83.33%), Postives = 95/108 (87.96%), Query Frame = 0


Query: 2   IDREKKAKKKATASGS-AAFPDEEPADAEADAVEEESTELEKVNESSEAPVVPVKTKTPK 61
           +DREKKAKKKAT SGS A  PDEEP D  ADAVEEESTE+EKVNE++EAP VPVKTK PK
Sbjct: 528 LDREKKAKKKATTSGSFATLPDEEPTD--ADAVEEESTEVEKVNETAEAP-VPVKTKIPK 587

Query: 62  EAGIRRPGRQRGLDSVPKVIRKRKKSINYLEWAGPAAAVAVVLLVILL 109
           E  +RRPGRQRGLDSVPKVIRKRKKSINYLEWAGPAAA A VLLVILL
Sbjct: 588 ETIVRRPGRQRGLDSVPKVIRKRKKSINYLEWAGPAAAGAAVLLVILL 632

[Close]

BLAST of Lsi04G020700 vs. ExPASy TrEMBL
Match: A0A5A7VD69 (Proton pump-interactor 1 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold255G00250 PE=4 SV=1)

HSP 1 Score: 151.4 bits (381), Expect = 2.7e-33
Identity = 90/108 (83.33%), Postives = 95/108 (87.96%), Query Frame = 0


Query: 2   IDREKKAKKKATASGS-AAFPDEEPADAEADAVEEESTELEKVNESSEAPVVPVKTKTPK 61
           +DREKKAKKKAT SGS A  PDEEP D  ADAVEEESTE+EKVNE++EAP VPVKTK PK
Sbjct: 397 LDREKKAKKKATTSGSFATLPDEEPTD--ADAVEEESTEVEKVNETAEAP-VPVKTKIPK 456

Query: 62  EAGIRRPGRQRGLDSVPKVIRKRKKSINYLEWAGPAAAVAVVLLVILL 109
           E  +RRPGRQRGLDSVPKVIRKRKKSINYLEWAGPAAA A VLLVILL
Sbjct: 457 ETIVRRPGRQRGLDSVPKVIRKRKKSINYLEWAGPAAAGAAVLLVILL 501

[Close]

BLAST of Lsi04G020700 vs. ExPASy TrEMBL
Match: A0A1S3BUN5 (proton pump-interactor 1 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103493484 PE=4 SV=1)

HSP 1 Score: 150.2 bits (378), Expect = 5.9e-33
Identity = 90/107 (84.11%), Postives = 94/107 (87.85%), Query Frame = 0


Query: 3   DREKKAKKKATASGS-AAFPDEEPADAEADAVEEESTELEKVNESSEAPVVPVKTKTPKE 62
           DREKKAKKKAT SGS A  PDEEP D  ADAVEEESTE+EKVNE++EAP VPVKTK PKE
Sbjct: 517 DREKKAKKKATTSGSFATLPDEEPTD--ADAVEEESTEVEKVNETAEAP-VPVKTKIPKE 576

Query: 63  AGIRRPGRQRGLDSVPKVIRKRKKSINYLEWAGPAAAVAVVLLVILL 109
             +RRPGRQRGLDSVPKVIRKRKKSINYLEWAGPAAA A VLLVILL
Sbjct: 577 TIVRRPGRQRGLDSVPKVIRKRKKSINYLEWAGPAAAGAAVLLVILL 620

[Close]

BLAST of Lsi04G020700 vs. ExPASy TrEMBL
Match: A0A6J1F7E9 (proton pump-interactor 1-like OS=Cucurbita moschata OX=3662 GN=LOC111442817 PE=4 SV=1)

HSP 1 Score: 143.3 bits (360), Expect = 7.2e-31
Identity = 81/105 (77.14%), Postives = 89/105 (84.76%), Query Frame = 0


Query: 3   DREKKAKKKATASGSAAFPDEEPADAEADAVEEESTELEKVNESSEAPVVPVKTKTPKEA 62
           DREKKAKKK TASGSA  PDEEP   EADAVEEESTELE VNES+EA  VPVK+K PKE 
Sbjct: 521 DREKKAKKKVTASGSATVPDEEP--VEADAVEEESTELENVNESAEAAPVPVKSKIPKET 580

Query: 63  GIRRPGRQRGLDSVPKVIRKRKKSINYLEWAGPAAAVAVVLLVIL 108
           GIR  GRQRGLDSVPK+IRKRKKSINY +WA PAAAV +V+L++L
Sbjct: 581 GIRSRGRQRGLDSVPKIIRKRKKSINYWDWAAPAAAVLLVILLVL 623

[Close]

BLAST of Lsi04G020700 vs. NCBI nr
Match: XP_038898028.1 (proton pump-interactor 1-like [Benincasa hispida])

HSP 1 Score: 153.3 bits (386), Expect = 1.4e-33
Identity = 89/106 (83.96%), Postives = 94/106 (88.68%), Query Frame = 0


Query: 3   DREKKAKKKATASGSAAFPDEEPADAEADAVEEESTELEKVNESSEAPVVPVKTKTPKEA 62
           DREKKAKKKATASGSA   DEEP DA+A AV+EESTELEKVNES+EAP VPVKTK PKE+
Sbjct: 520 DREKKAKKKATASGSATLTDEEPVDADAGAVQEESTELEKVNESAEAP-VPVKTKIPKES 579

Query: 63  GIRRPGRQRGLDSVPKVIRKRKKSINYLEWAGPAAAVAVVLLVILL 109
           G R  GRQRGLDSVPKVIRKRKKSINYL+WAGPAAA A VLLVILL
Sbjct: 580 GGRNRGRQRGLDSVPKVIRKRKKSINYLDWAGPAAAGAAVLLVILL 624

[Close]

BLAST of Lsi04G020700 vs. NCBI nr
Match: KAE8649841.1 (hypothetical protein Csa_012471 [Cucumis sativus])

HSP 1 Score: 152.5 bits (384), Expect = 2.5e-33
Identity = 91/108 (84.26%), Postives = 95/108 (87.96%), Query Frame = 0


Query: 2   IDREKKAKKKATASGS-AAFPDEEPADAEADAVEEESTELEKVNESSEAPVVPVKTKTPK 61
           IDREKKAKKKAT SGS A  PDEEP D  ADAVEEESTE+EKVNE++EAP VPVKTK PK
Sbjct: 525 IDREKKAKKKATTSGSFATLPDEEPTD--ADAVEEESTEVEKVNETAEAP-VPVKTKIPK 584

Query: 62  EAGIRRPGRQRGLDSVPKVIRKRKKSINYLEWAGPAAAVAVVLLVILL 109
           E  +RRPGRQRGLDSVPKVIRKRKKSINYLEWAGPAAA A VLLVILL
Sbjct: 585 ETVVRRPGRQRGLDSVPKVIRKRKKSINYLEWAGPAAAGAAVLLVILL 629

[Close]

BLAST of Lsi04G020700 vs. NCBI nr
Match: KAA0064326.1 (proton pump-interactor 1 isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 151.4 bits (381), Expect = 5.5e-33
Identity = 90/108 (83.33%), Postives = 95/108 (87.96%), Query Frame = 0


Query: 2   IDREKKAKKKATASGS-AAFPDEEPADAEADAVEEESTELEKVNESSEAPVVPVKTKTPK 61
           +DREKKAKKKAT SGS A  PDEEP D  ADAVEEESTE+EKVNE++EAP VPVKTK PK
Sbjct: 397 LDREKKAKKKATTSGSFATLPDEEPTD--ADAVEEESTEVEKVNETAEAP-VPVKTKIPK 456

Query: 62  EAGIRRPGRQRGLDSVPKVIRKRKKSINYLEWAGPAAAVAVVLLVILL 109
           E  +RRPGRQRGLDSVPKVIRKRKKSINYLEWAGPAAA A VLLVILL
Sbjct: 457 ETIVRRPGRQRGLDSVPKVIRKRKKSINYLEWAGPAAAGAAVLLVILL 501

[Close]

BLAST of Lsi04G020700 vs. NCBI nr
Match: TYK20260.1 (proton pump-interactor 1 isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 151.4 bits (381), Expect = 5.5e-33
Identity = 90/108 (83.33%), Postives = 95/108 (87.96%), Query Frame = 0


Query: 2   IDREKKAKKKATASGS-AAFPDEEPADAEADAVEEESTELEKVNESSEAPVVPVKTKTPK 61
           +DREKKAKKKAT SGS A  PDEEP D  ADAVEEESTE+EKVNE++EAP VPVKTK PK
Sbjct: 528 LDREKKAKKKATTSGSFATLPDEEPTD--ADAVEEESTEVEKVNETAEAP-VPVKTKIPK 587

Query: 62  EAGIRRPGRQRGLDSVPKVIRKRKKSINYLEWAGPAAAVAVVLLVILL 109
           E  +RRPGRQRGLDSVPKVIRKRKKSINYLEWAGPAAA A VLLVILL
Sbjct: 588 ETIVRRPGRQRGLDSVPKVIRKRKKSINYLEWAGPAAAGAAVLLVILL 632

[Close]

BLAST of Lsi04G020700 vs. NCBI nr
Match: XP_011654071.2 (proton pump-interactor 1 [Cucumis sativus])

HSP 1 Score: 150.6 bits (379), Expect = 9.3e-33
Identity = 90/107 (84.11%), Postives = 94/107 (87.85%), Query Frame = 0


Query: 3   DREKKAKKKATASGS-AAFPDEEPADAEADAVEEESTELEKVNESSEAPVVPVKTKTPKE 62
           DREKKAKKKAT SGS A  PDEEP D  ADAVEEESTE+EKVNE++EAP VPVKTK PKE
Sbjct: 517 DREKKAKKKATTSGSFATLPDEEPTD--ADAVEEESTEVEKVNETAEAP-VPVKTKIPKE 576

Query: 63  AGIRRPGRQRGLDSVPKVIRKRKKSINYLEWAGPAAAVAVVLLVILL 109
             +RRPGRQRGLDSVPKVIRKRKKSINYLEWAGPAAA A VLLVILL
Sbjct: 577 TVVRRPGRQRGLDSVPKVIRKRKKSINYLEWAGPAAAGAAVLLVILL 620

[Close]

BLAST of Lsi04G020700 vs. TAIR 10
Match: AT4G27500.1 (proton pump interactor 1 )

HSP 1 Score: 49.3 bits (116), Expect = 2.7e-06
Identity = 47/122 (38.52%), Postives = 67/122 (54.92%), Query Frame = 0


Query: 2   IDREKKAKKKATASGSAAFPDEEPADAEADAVEEE----------STELEKVNESS---- 61
           ++R+KK  +KA A   AA   ++ A+ +    +E+           TE E+V E+S    
Sbjct: 487 MERKKKLAEKAAA--KAAIRAQKEAEKKEKKEQEKKAKKKTGGNTETETEEVPEASEEEI 546

Query: 62  EAPV---VPVKTKTPKEAGIRRPGRQRGLDSVPKVIRKRKKSINYLEWAGPAAAVAVVLL 107
           EAPV    P K K  KE  IR   R RG +++P+ I KRKKS NY  +A PAA V ++LL
Sbjct: 547 EAPVQEEKPQKEKVFKEKPIRNRTRGRGPETIPRAILKRKKSTNYWVYAAPAALVVLLLL 606

The following BLAST results are available for this feature:

BLAST of Lsi04G020700 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O23144	3.8e-05	38.52	Proton pump-interactor 1 OS=Arabidopsis thaliana OX=3702 GN=PPI1 PE=1 SV=2	[more]
B3H4K7	9.5e-04	34.58	Proton pump-interactor 2 OS=Arabidopsis thaliana OX=3702 GN=PPI2 PE=2 SV=1	[more]

BLAST of Lsi04G020700 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L261	1.2e-33	84.26	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G637150 PE=4 SV=1	[more]
A0A5D3D9V7	2.7e-33	83.33	Proton pump-interactor 1 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5...	[more]
A0A5A7VD69	2.7e-33	83.33	Proton pump-interactor 1 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6...	[more]
A0A1S3BUN5	5.9e-33	84.11	proton pump-interactor 1 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103493484 PE=4...	[more]
A0A6J1F7E9	7.2e-31	77.14	proton pump-interactor 1-like OS=Cucurbita moschata OX=3662 GN=LOC111442817 PE=4...	[more]

BLAST of Lsi04G020700 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038898028.1	1.4e-33	83.96	proton pump-interactor 1-like [Benincasa hispida]	[more]
KAE8649841.1	2.5e-33	84.26	hypothetical protein Csa_012471 [Cucumis sativus]	[more]
KAA0064326.1	5.5e-33	83.33	proton pump-interactor 1 isoform X1 [Cucumis melo var. makuwa]	[more]
TYK20260.1	5.5e-33	83.33	proton pump-interactor 1 isoform X1 [Cucumis melo var. makuwa]	[more]
XP_011654071.2	9.3e-33	84.11	proton pump-interactor 1 [Cucumis sativus]	[more]

BLAST of Lsi04G020700 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G27500.1	2.7e-06	38.52	proton pump interactor 1	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 59..75
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..75

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi04G020700.1	Lsi04G020700.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane