Lsi04G020410 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi04G020410
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	E3 SUMO-protein ligase MMS21
Location	chr04: 27502028 .. 27505177 (-)
RNA-Seq Expression	Lsi04G020410
Synteny	Lsi04G020410

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GGTTCTCCAAAACTCGGACCAGTTTGTTTCCCCAAGCGGGTATTCAATATTTCGCGCTTTAAAAGTTAAAATTCGAACCATCGCCCGAGGAGTTTCTGTTTCGGCAATCTCTTCTTCAAAGGAATGGCATCCGCTTCCGATTCCCGTTCAAGCGGCGTTTCCGGTAGAATCAAATCCGCCGCCACTATTATGCACTCCGAGAATCAATCCCTCCTTGCCGTAACTTGTTTAATACTACTCTCTTACTTCTATCTCTGCTTTCAATTTGTTGTTCTGATTGATAAACGAAATATTCTGATTGTTCGAGAAAATGAAGTTGCTGTTGCTCAATAAACGTTGTTTTAGCTTTAACATCTCGATTTCCTGTTATTGAGTGTCAGTTCTAATGCTGTATTCCCTTCATTTTGCGCTTTTAACTGGGGTTTTCTAGATTTCTGAAAGCTAAAACGTCCGGGCCAAGATTATCTTATTGAAAAGAATGATTTATAAGGAATTTGACTAATGATGATAGGTTATCTGTTAACTTTTGTGATTGGATTTTACTGTCTTAATTTCAGGAATTGCGGAAGGCGCTGATTATGATGAAGGAGATTGGGGTGGATTTGGAGAGGGACAATCAGTCCAGGATGGTATTGTGAATTATGGACTTTAATTTCGAGCATCATAAATGATTGTCATTTGTTATTTTGGGAAATAATGTTTTTGTGTTAGGATAAAATCTTGTAATAAAATTTCATAAAAAAATAAAATAAAGAAAGATCTTGTAATAAAATACTAAGAAGCAATGTCAACATAACTTTGAAAGATGTTCTCTAGGACTCTTTTCGCTTATTTTAACATCTTTGGAGTTTCTTTTTTAGTTAGTTGAAAATAGAATAGCAGATTGTATACAATATGCTGCACATATATCTTGTTGGAACTTTCATTATAAACCAGCCACTTTGTCGACCGTTCAAAATTCAAATATTTGAAACTCTTATTTCCATTTAGTGATTGTGCTACCAACATACATTTTTGTTTTTGACAGACTTTCTGTGGATGACACGATATAAATAAGATTTATTTGAACCGATTCCATGCTTAAAAATTCTAATATTGGTTTCAGTAGGTGTACATTTAATTTAACTAATATCTATACCACATGCACCTTTTGGTGGATTTTCTGCTTCTGTCTATTAGATCCAATCTGAAGATAATTGTGAATCGCTGTGGTTTGGAACTCTACCAATAATCTACTTGGTATTTGAACATTATCTTAGGTCAAGGAGCTTGAAAATTCTGTTGTCGAGCTGTTGGGTACTTATGAAAACTGTAACAACTTTTCTTCTGCAATTCAGTCGGTTGGAAATAGATATGAACCAAAAGAAGAGGTGCTTAAATCTCTTTCCATGTTGAATTTTCCACTACACATTCTTTTGTTAATTTTCATTGTGTAGGTTCCTTCCTTTTCAGACGTAATATTTTATGTTGATATTTTAGTTAACAGATTTTGAGAAACTACTTGACAATGAAGTTGCAAAACTCAGCGAAAATTCATCTTCAGATTTGCTGAACCATTCAATAATTCGGCAATTTAGAGAAGCCATTTGGGTATGCATCTTCATCTCCTTTTCTATGGTCTGGGAAGGGCCTTGGCACCCATAAGAAAATAGATCAGAAAGCCAGGGGAAAATATTTCCCTTCACTTTTATTTCTGATTATAGTAAAAAAATTTATTGTGTTATCTCACTTTTGTGCCTTTCTATTGCTCGGGCAACAGCACTGGTTATGCATGGCAGCAACAAAAAGTCCTCAAATCTGTGCAAATAATTATCCTGAAACTGTGAAAATGCCCAAAAGAAAGTACATGAGTAGTAAACAAGAGAAACTATTTTACCTGTTTGCAATTTACAAATGTTTGGAAAGCATTTTATTACTCATCCAATTATAGATTTAATTCTTTCCAGTGCTTTAGCCAATCTTGGTATTCCTGCAGAATGTTCATCATGCAGGACAACCGATGCCAGGTGAGGAGCAGGAGGACATTGTGATGACCAGTACTCAGTGTAATCTATTGAATGTCACTTGCCCGTTAAGTGGAAAGCCTGTCACTGAATTAGAAGAACCTGTTCGCAGGTATATTGCAACTAAATATATTTTGATGAATAATTAAGAATTGTTATCATTTCTGTTACTATTGAAAAAGATGTCTGCCCATCCATTTGCTGTGTAGTGTGTACTGACGGCATGGATGTTCAAAGGGAATGGCTACATGCTTGTGTTATTTTCTCTGGTTACATTTTTCAAGTGGGTGCCTGAAAATGGAATGAACTAGTCTGCCACTTTGATCTTCTTCTTCCTCCTCATATGTTTCAGAGCTGAGTCTTGAAATTGTATATTTTTGTTCTTTAAACTATTAATCGTTTTAGGTTGTGGAGAAGGTTCATTCACATGGATATGGCCCTTTTTTTTTTTCCTTGGTTTTCTGTTATGGATCAGTACAAGCATCAGACCATCGTGCATTTAAACACTTCTTTAAGTCTCTTTTTGTCTCTACCTTCGGCAATTTGTAGTATGGAATGCAAGCACATATATGAAAAGGCAGCCATAATGCAGTACCTTAAATCCAAGAAATCTCGCGCTCAATGCCCGGTTGCAGGTATTCGGATCTACTACATTTCATATACTACATACAGAATGTTTACATTGGCTCGCGCTCATCTCACTTGATCTCTCTGTCCTTGTAGCCTGTCCTAAGATGTTGCAGCCCGAAAAGGTTGTGCCTGATCCGTTCTTACAGATTGAAATTGATGAACTGCGAAAAATGTCTAGGCATTCTGCAAGGATACAGGACTTCACAGAGCTTGATGCAGATTAGCACCACTGAAGTAGTTTCTATTCCTCTTTTTTGTTGTTTTTATTGTCATTGTCATCGTCGTCATTGTCTTCAGGGTCACTCTTTGGATCAAATGTTTTGCTTGGTAAGTTAAGCCAGTTGGGGGGTGTATATGGTATCTATAGTTTGTCTATATTTTCTCTTCACAATGAGTTGAATGGAGAAATGTGGCTTCATTGACAAATTGCTATTGCCCTTTATATTTTGAGTTGTTCTTTTCCTCCTGCGCCAGTGTAAATAACGTTTGTATTTGCAAACAAAATTTTGACTCTAAA

mRNA sequence

GGTTCTCCAAAACTCGGACCAGTTTGTTTCCCCAAGCGGGTATTCAATATTTCGCGCTTTAAAAGTTAAAATTCGAACCATCGCCCGAGGAGTTTCTGTTTCGGCAATCTCTTCTTCAAAGGAATGGCATCCGCTTCCGATTCCCGTTCAAGCGGCGTTTCCGGTAGAATCAAATCCGCCGCCACTATTATGCACTCCGAGAATCAATCCCTCCTTGCCGAATTGCGGAAGGCGCTGATTATGATGAAGGAGATTGGGGTGGATTTGGAGAGGGACAATCAGTCCAGGATGGTCAAGGAGCTTGAAAATTCTGTTGTCGAGCTGTTGGGTACTTATGAAAACTGTAACAACTTTTCTTCTGCAATTCAGTCGGTTGGAAATAGATATGAACCAAAAGAAGAGTTAACAGATTTTGAGAAACTACTTGACAATGAAGTTGCAAAACTCAGCGAAAATTCATCTTCAGATTTGCTGAACCATTCAATAATTCGGCAATTTAGAGAAGCCATTTGGAATGTTCATCATGCAGGACAACCGATGCCAGGTGAGGAGCAGGAGGACATTGTGATGACCAGTACTCAGTGTAATCTATTGAATGTCACTTGCCCGTTAAGTGGAAAGCCTGTCACTGAATTAGAAGAACCTGTTCGCAGTATGGAATGCAAGCACATATATGAAAAGGCAGCCATAATGCAGTACCTTAAATCCAAGAAATCTCGCGCTCAATGCCCGGTTGCAGCCTGTCCTAAGATGTTGCAGCCCGAAAAGGTTGTGCCTGATCCGTTCTTACAGATTGAAATTGATGAACTGCGAAAAATGTCTAGGCATTCTGCAAGGATACAGGACTTCACAGAGCTTGATGCAGATTAGCACCACTGAAGTAGTTTCTATTCCTCTTTTTTGTTGTTTTTATTGTCATTGTCATCGTCGTCATTGTCTTCAGGGTCACTCTTTGGATCAAATGTTTTGCTTGGTAAGTTAAGCCAGTTGGGGGGTGTATATGGTATCTATAGTTTGTCTATATTTTCTCTTCACAATGAGTTGAATGGAGAAATGTGGCTTCATTGACAAATTGCTATTGCCCTTTATATTTTGAGTTGTTCTTTTCCTCCTGCGCCAGTGTAAATAACGTTTGTATTTGCAAACAAAATTTTGACTCTAAA

Coding sequence (CDS)

ATGGCATCCGCTTCCGATTCCCGTTCAAGCGGCGTTTCCGGTAGAATCAAATCCGCCGCCACTATTATGCACTCCGAGAATCAATCCCTCCTTGCCGAATTGCGGAAGGCGCTGATTATGATGAAGGAGATTGGGGTGGATTTGGAGAGGGACAATCAGTCCAGGATGGTCAAGGAGCTTGAAAATTCTGTTGTCGAGCTGTTGGGTACTTATGAAAACTGTAACAACTTTTCTTCTGCAATTCAGTCGGTTGGAAATAGATATGAACCAAAAGAAGAGTTAACAGATTTTGAGAAACTACTTGACAATGAAGTTGCAAAACTCAGCGAAAATTCATCTTCAGATTTGCTGAACCATTCAATAATTCGGCAATTTAGAGAAGCCATTTGGAATGTTCATCATGCAGGACAACCGATGCCAGGTGAGGAGCAGGAGGACATTGTGATGACCAGTACTCAGTGTAATCTATTGAATGTCACTTGCCCGTTAAGTGGAAAGCCTGTCACTGAATTAGAAGAACCTGTTCGCAGTATGGAATGCAAGCACATATATGAAAAGGCAGCCATAATGCAGTACCTTAAATCCAAGAAATCTCGCGCTCAATGCCCGGTTGCAGCCTGTCCTAAGATGTTGCAGCCCGAAAAGGTTGTGCCTGATCCGTTCTTACAGATTGAAATTGATGAACTGCGAAAAATGTCTAGGCATTCTGCAAGGATACAGGACTTCACAGAGCTTGATGCAGATTAG

Protein sequence

MASASDSRSSGVSGRIKSAATIMHSENQSLLAELRKALIMMKEIGVDLERDNQSRMVKELENSVVELLGTYENCNNFSSAIQSVGNRYEPKEELTDFEKLLDNEVAKLSENSSSDLLNHSIIRQFREAIWNVHHAGQPMPGEEQEDIVMTSTQCNLLNVTCPLSGKPVTELEEPVRSMECKHIYEKAAIMQYLKSKKSRAQCPVAACPKMLQPEKVVPDPFLQIEIDELRKMSRHSARIQDFTELDAD

Homology

[Close]

BLAST of Lsi04G020410 vs. ExPASy Swiss-Prot
Match: Q8GYH7 (E3 SUMO-protein ligase MMS21 OS=Arabidopsis thaliana OX=3702 GN=MMS21 PE=1 SV=1)

HSP 1 Score: 276.6 bits (706), Expect = 2.8e-73
Identity = 141/244 (57.79%), Postives = 187/244 (76.64%), Query Frame = 0


Query: 4   ASDSRSSGVSGRIKSAATIMHSENQSLLAELRKALIMMKEIGVDLERDNQSRMVKELENS 63
           AS S S GV+GRI++A+ ++ S+N S LA++RKA+ MMK I V LE++NQ+  VK+LENS
Sbjct: 2   ASASSSDGVAGRIQNASLVLVSDNSSTLADIRKAVAMMKNIAVQLEKENQTDKVKDLENS 61

Query: 64  VVELLGTYENCNNFSSAIQSVGNRYEPKEELTDFEKLLDNEVAKLSENSSSDLLNHSIIR 123
           V ELL  + +CN+ S+AIQSV NRY+P E+LTDF+KLLD+E  KL    SS   N  ++R
Sbjct: 62  VAELLDLHSDCNHRSTAIQSVANRYQPVEQLTDFKKLLDDEFTKLKATPSSVPQNDHLMR 121

Query: 124 QFREAIWNVHHAGQPMPGEEQEDIVMTSTQCNLLNVTCPLSGKPVTELEEPVRSMECKHI 183
           QFREA+WNVHHAG+PMPG++ EDIVMTSTQC LLN+TCPLSGKPVTEL +PVRSM+C+H+
Sbjct: 122 QFREAVWNVHHAGEPMPGDDDEDIVMTSTQCPLLNMTCPLSGKPVTELADPVRSMDCRHV 181

Query: 184 YEKAAIMQYLKSKKSRAQCPVAACPKMLQPEKVVPDPFLQIEIDELRKMSRHSAR---IQ 243
           YEK+ I+ Y+ +  + A CPVA C   LQ  KV+ D  L+ EI+E+R +++ S R   I+
Sbjct: 182 YEKSVILHYIVNNPN-ANCPVAGCRGKLQNSKVICDAMLKFEIEEMRSLNKQSNRAEVIE 241

Query: 244 DFTE 245
           DFTE
Sbjct: 242 DFTE 244

[Close]

BLAST of Lsi04G020410 vs. ExPASy TrEMBL
Match: A0A5D3DWX9 (E3 SUMO-protein ligase MMS21 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold384G001530 PE=3 SV=1)

HSP 1 Score: 430.3 bits (1105), Expect = 5.6e-117
Identity = 220/248 (88.71%), Postives = 233/248 (93.95%), Query Frame = 0


Query: 1   MASASDSRSSGVSGRIKSAATIMHSENQSLLAELRKALIMMKEIGVDLERDNQSRMVKEL 60
           MASASDSRS+GV+GRIKSAATIMHSENQSLLAELRK LIMMKEIGVDLE++NQ++MVKEL
Sbjct: 1   MASASDSRSTGVTGRIKSAATIMHSENQSLLAELRKTLIMMKEIGVDLEKENQNKMVKEL 60

Query: 61  ENSVVELLGTYENCNNFSSAIQSVGNRYEPKEELTDFEKLLDNEVAKLSENSSSDLLNHS 120
           E S+VELL  YENCNNFSSAIQSVGN YEPKEELTDFEKLLD+EVAK+S NSSS+  NH 
Sbjct: 61  EKSMVELLSAYENCNNFSSAIQSVGNTYEPKEELTDFEKLLDDEVAKVSVNSSSNFANHP 120

Query: 121 IIRQFREAIWNVHHAGQPMPGEEQEDIVMTSTQCNLLNVTCPLSGKPVTELEEPVRSMEC 180
           IIRQFREAIWNVHHAGQPM GEEQED+VMTSTQCNLLNVTCPLSGKPVTEL EPVRS EC
Sbjct: 121 IIRQFREAIWNVHHAGQPMAGEEQEDVVMTSTQCNLLNVTCPLSGKPVTELAEPVRSAEC 180

Query: 181 KHIYEKAAIMQYLKSKKSRAQCPVAACPKMLQPEKVVPDPFLQIEIDELRKMSRHSARIQ 240
           KHIYEKAAIMQYL SKKSRAQCPVAACPKMLQP+KVV DPFL+IEIDELRKMSRHS RIQ
Sbjct: 181 KHIYEKAAIMQYLNSKKSRAQCPVAACPKMLQPDKVVLDPFLEIEIDELRKMSRHSERIQ 240

Query: 241 DFTELDAD 249
           DFTELDAD
Sbjct: 241 DFTELDAD 248

[Close]

BLAST of Lsi04G020410 vs. ExPASy TrEMBL
Match: A0A1S3CLF9 (E3 SUMO-protein ligase MMS21 OS=Cucumis melo OX=3656 GN=LOC103501788 PE=3 SV=1)

HSP 1 Score: 430.3 bits (1105), Expect = 5.6e-117
Identity = 220/248 (88.71%), Postives = 233/248 (93.95%), Query Frame = 0


Query: 1   MASASDSRSSGVSGRIKSAATIMHSENQSLLAELRKALIMMKEIGVDLERDNQSRMVKEL 60
           MASASDSRS+GV+GRIKSAATIMHSENQSLLAELRK LIMMKEIGVDLE++NQ++MVKEL
Sbjct: 1   MASASDSRSTGVTGRIKSAATIMHSENQSLLAELRKTLIMMKEIGVDLEKENQNKMVKEL 60

Query: 61  ENSVVELLGTYENCNNFSSAIQSVGNRYEPKEELTDFEKLLDNEVAKLSENSSSDLLNHS 120
           E S+VELL  YENCNNFSSAIQSVGN YEPKEELTDFEKLLD+EVAK+S NSSS+  NH 
Sbjct: 61  EKSMVELLSAYENCNNFSSAIQSVGNTYEPKEELTDFEKLLDDEVAKVSVNSSSNFANHP 120

Query: 121 IIRQFREAIWNVHHAGQPMPGEEQEDIVMTSTQCNLLNVTCPLSGKPVTELEEPVRSMEC 180
           IIRQFREAIWNVHHAGQPM GEEQED+VMTSTQCNLLNVTCPLSGKPVTEL EPVRS EC
Sbjct: 121 IIRQFREAIWNVHHAGQPMAGEEQEDVVMTSTQCNLLNVTCPLSGKPVTELAEPVRSAEC 180

Query: 181 KHIYEKAAIMQYLKSKKSRAQCPVAACPKMLQPEKVVPDPFLQIEIDELRKMSRHSARIQ 240
           KHIYEKAAIMQYL SKKSRAQCPVAACPKMLQP+KVV DPFL+IEIDELRKMSRHS RIQ
Sbjct: 181 KHIYEKAAIMQYLNSKKSRAQCPVAACPKMLQPDKVVLDPFLEIEIDELRKMSRHSERIQ 240

Query: 241 DFTELDAD 249
           DFTELDAD
Sbjct: 241 DFTELDAD 248

[Close]

BLAST of Lsi04G020410 vs. ExPASy TrEMBL
Match: A0A6J1CCS6 (E3 SUMO-protein ligase MMS21 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111010470 PE=3 SV=1)

HSP 1 Score: 429.1 bits (1102), Expect = 1.3e-116
Identity = 217/248 (87.50%), Postives = 234/248 (94.35%), Query Frame = 0


Query: 1   MASASDSRSSGVSGRIKSAATIMHSENQSLLAELRKALIMMKEIGVDLERDNQSRMVKEL 60
           MAS SD+RSS VSGRIKSAAT+MHSENQSLLAE+RK LIMMKEIG+DLERDNQS MVK+L
Sbjct: 1   MASTSDTRSSAVSGRIKSAATMMHSENQSLLAEIRKLLIMMKEIGMDLERDNQSTMVKKL 60

Query: 61  ENSVVELLGTYENCNNFSSAIQSVGNRYEPKEELTDFEKLLDNEVAKLSENSSSDLLNHS 120
           EN+VVELL TYENC+NFSSAIQSVGN YEP+EELTDFEKLLD+EVAK+SENSSS+L NHS
Sbjct: 61  ENAVVELLSTYENCDNFSSAIQSVGNIYEPREELTDFEKLLDDEVAKVSENSSSNLQNHS 120

Query: 121 IIRQFREAIWNVHHAGQPMPGEEQEDIVMTSTQCNLLNVTCPLSGKPVTELEEPVRSMEC 180
           IIR+FREAIWNVHHAGQPMPGEEQEDIVMTSTQCNLLNVTCPLSGKPVTEL +PVRSMEC
Sbjct: 121 IIRKFREAIWNVHHAGQPMPGEEQEDIVMTSTQCNLLNVTCPLSGKPVTELAQPVRSMEC 180

Query: 181 KHIYEKAAIMQYLKSKKSRAQCPVAACPKMLQPEKVVPDPFLQIEIDELRKMSRHSARIQ 240
           KH+YEK A+MQYL+SK SRAQCPVAACPKMLQPEKV PDPFL IEIDELRK SRHS RIQ
Sbjct: 181 KHVYEKEAVMQYLRSKNSRAQCPVAACPKMLQPEKVTPDPFLLIEIDELRKTSRHSVRIQ 240

Query: 241 DFTELDAD 249
           DFTELDA+
Sbjct: 241 DFTELDAE 248

[Close]

BLAST of Lsi04G020410 vs. ExPASy TrEMBL
Match: A0A6J1FRP6 (E3 SUMO-protein ligase MMS21 OS=Cucurbita moschata OX=3662 GN=LOC111446631 PE=3 SV=1)

HSP 1 Score: 429.1 bits (1102), Expect = 1.3e-116
Identity = 219/248 (88.31%), Postives = 235/248 (94.76%), Query Frame = 0


Query: 1   MASASDSRSSGVSGRIKSAATIMHSENQSLLAELRKALIMMKEIGVDLERDNQSRMVKEL 60
           MAS S+S S+GVS RIKSAATIM+SENQSLLAELRK+LIMMKEIGV+LERDNQSRMVKEL
Sbjct: 1   MASTSNSNSTGVSERIKSAATIMNSENQSLLAELRKSLIMMKEIGVELERDNQSRMVKEL 60

Query: 61  ENSVVELLGTYENCNNFSSAIQSVGNRYEPKEELTDFEKLLDNEVAKLSENSSSDLLNHS 120
           ENSVVELLGTYE C+NFSSAIQSVGN YEP+EELTDFEKLLD+EVAK+SENSSS+L NHS
Sbjct: 61  ENSVVELLGTYEKCSNFSSAIQSVGNIYEPQEELTDFEKLLDDEVAKVSENSSSNLQNHS 120

Query: 121 IIRQFREAIWNVHHAGQPMPGEEQEDIVMTSTQCNLLNVTCPLSGKPVTELEEPVRSMEC 180
           IIRQFREAIWNVHHAGQPMPGEEQED+VMTSTQCNLLNVTCPLSGKPVTEL EPVRS EC
Sbjct: 121 IIRQFREAIWNVHHAGQPMPGEEQEDVVMTSTQCNLLNVTCPLSGKPVTELAEPVRSTEC 180

Query: 181 KHIYEKAAIMQYLKSKKSRAQCPVAACPKMLQPEKVVPDPFLQIEIDELRKMSRHSARIQ 240
           KH+YEK AIMQYLKSK+SRAQCPVAACPKMLQPEKV+ DPFL IEIDELRK S+HS RIQ
Sbjct: 181 KHVYEKVAIMQYLKSKRSRAQCPVAACPKMLQPEKVISDPFLPIEIDELRKTSKHSVRIQ 240

Query: 241 DFTELDAD 249
           DFTE+DAD
Sbjct: 241 DFTEVDAD 248

[Close]

BLAST of Lsi04G020410 vs. ExPASy TrEMBL
Match: A0A6J1IXZ2 (E3 SUMO-protein ligase MMS21 OS=Cucurbita maxima OX=3661 GN=LOC111480943 PE=3 SV=1)

HSP 1 Score: 426.4 bits (1095), Expect = 8.2e-116
Identity = 218/246 (88.62%), Postives = 232/246 (94.31%), Query Frame = 0


Query: 3   SASDSRSSGVSGRIKSAATIMHSENQSLLAELRKALIMMKEIGVDLERDNQSRMVKELEN 62
           S S+S S+GVS RIKSAATIM+SENQSLLAELRK+LIMMKEIGVDLERDNQSRMVKELEN
Sbjct: 5   SNSNSNSTGVSERIKSAATIMNSENQSLLAELRKSLIMMKEIGVDLERDNQSRMVKELEN 64

Query: 63  SVVELLGTYENCNNFSSAIQSVGNRYEPKEELTDFEKLLDNEVAKLSENSSSDLLNHSII 122
           SVVELL TYE C+NFSSAIQSVGN YEP+EELTDFEKLLDNEVAK+SENSSS+L NHSII
Sbjct: 65  SVVELLSTYEKCSNFSSAIQSVGNIYEPQEELTDFEKLLDNEVAKVSENSSSNLQNHSII 124

Query: 123 RQFREAIWNVHHAGQPMPGEEQEDIVMTSTQCNLLNVTCPLSGKPVTELEEPVRSMECKH 182
           RQFREAIWNVHHAGQPMPGEEQED+VMTSTQCNLLNVTCPLSGKPVTEL EPVRS ECKH
Sbjct: 125 RQFREAIWNVHHAGQPMPGEEQEDVVMTSTQCNLLNVTCPLSGKPVTELAEPVRSTECKH 184

Query: 183 IYEKAAIMQYLKSKKSRAQCPVAACPKMLQPEKVVPDPFLQIEIDELRKMSRHSARIQDF 242
           +YEK AIMQYLKSK+SRAQCPVAACPKMLQPEKV+ DPFL IEIDELRK S+HS RIQDF
Sbjct: 185 VYEKVAIMQYLKSKRSRAQCPVAACPKMLQPEKVISDPFLPIEIDELRKTSKHSVRIQDF 244

Query: 243 TELDAD 249
           TE+DAD
Sbjct: 245 TEVDAD 250

[Close]

BLAST of Lsi04G020410 vs. NCBI nr
Match: XP_038897059.1 (E3 SUMO-protein ligase MMS21 isoform X1 [Benincasa hispida])

HSP 1 Score: 441.0 bits (1133), Expect = 6.6e-120
Identity = 226/248 (91.13%), Postives = 239/248 (96.37%), Query Frame = 0


Query: 1   MASASDSRSSGVSGRIKSAATIMHSENQSLLAELRKALIMMKEIGVDLERDNQSRMVKEL 60
           MASASDSRS+GVSGRIKSAATIMHSENQSLLAELRKALIMMKEIGV+LERDNQSRMVKEL
Sbjct: 1   MASASDSRSNGVSGRIKSAATIMHSENQSLLAELRKALIMMKEIGVELERDNQSRMVKEL 60

Query: 61  ENSVVELLGTYENCNNFSSAIQSVGNRYEPKEELTDFEKLLDNEVAKLSENSSSDLLNHS 120
           ENSVVELL TYENCNNFS AIQSVGN YEPKEELTDF KLLD+EVAK+S++SSS+L+NHS
Sbjct: 61  ENSVVELLSTYENCNNFSLAIQSVGNIYEPKEELTDFGKLLDDEVAKVSQDSSSNLMNHS 120

Query: 121 IIRQFREAIWNVHHAGQPMPGEEQEDIVMTSTQCNLLNVTCPLSGKPVTELEEPVRSMEC 180
           IIRQFREA+WNVHHAGQPMPGEE+EDIVMTSTQCNLLNVTCPLSGKPV EL EPVRS EC
Sbjct: 121 IIRQFREAVWNVHHAGQPMPGEEKEDIVMTSTQCNLLNVTCPLSGKPVIELTEPVRSAEC 180

Query: 181 KHIYEKAAIMQYLKSKKSRAQCPVAACPKMLQPEKVVPDPFLQIEIDELRKMSRHSARIQ 240
           KH+YEKAAIMQYLKSKKSRAQCPVAACPKMLQ +KVVPDPFLQIEIDELRK+SRHS RIQ
Sbjct: 181 KHVYEKAAIMQYLKSKKSRAQCPVAACPKMLQSDKVVPDPFLQIEIDELRKISRHSDRIQ 240

Query: 241 DFTELDAD 249
           DFTELDAD
Sbjct: 241 DFTELDAD 248

[Close]

BLAST of Lsi04G020410 vs. NCBI nr
Match: XP_023525588.1 (E3 SUMO-protein ligase MMS21 [Cucurbita pepo subsp. pepo] >KAG7037426.1 E3 SUMO-protein ligase MMS21 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 432.6 bits (1111), Expect = 2.3e-117
Identity = 221/248 (89.11%), Postives = 235/248 (94.76%), Query Frame = 0


Query: 1   MASASDSRSSGVSGRIKSAATIMHSENQSLLAELRKALIMMKEIGVDLERDNQSRMVKEL 60
           MAS S+S S+GVS RIKSAATIM+SENQSLLAELRK+LIMMKEIGVDLERDNQSRMVKEL
Sbjct: 1   MASTSNSNSTGVSERIKSAATIMNSENQSLLAELRKSLIMMKEIGVDLERDNQSRMVKEL 60

Query: 61  ENSVVELLGTYENCNNFSSAIQSVGNRYEPKEELTDFEKLLDNEVAKLSENSSSDLLNHS 120
           ENSVVELLGTYE C+NFSSAIQSVGN YEP+EELTDFEKLLDNEVAK+SENSSS+L NHS
Sbjct: 61  ENSVVELLGTYEKCSNFSSAIQSVGNIYEPQEELTDFEKLLDNEVAKVSENSSSNLQNHS 120

Query: 121 IIRQFREAIWNVHHAGQPMPGEEQEDIVMTSTQCNLLNVTCPLSGKPVTELEEPVRSMEC 180
           IIRQFREAIWNVHHAGQPMPGEEQED+VMTSTQCNLLNVTCPLSGKPVTEL EPVRS EC
Sbjct: 121 IIRQFREAIWNVHHAGQPMPGEEQEDVVMTSTQCNLLNVTCPLSGKPVTELAEPVRSTEC 180

Query: 181 KHIYEKAAIMQYLKSKKSRAQCPVAACPKMLQPEKVVPDPFLQIEIDELRKMSRHSARIQ 240
           KH+YEK AIMQYLKSK+SRAQCPVAACPKMLQPEKV+ DPFL IEIDELRK S+HS RIQ
Sbjct: 181 KHVYEKVAIMQYLKSKRSRAQCPVAACPKMLQPEKVISDPFLPIEIDELRKTSKHSVRIQ 240

Query: 241 DFTELDAD 249
           DFTE+DAD
Sbjct: 241 DFTEVDAD 248

[Close]

BLAST of Lsi04G020410 vs. NCBI nr
Match: KAG6607898.1 (E3 SUMO-protein ligase MMS21, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 432.2 bits (1110), Expect = 3.1e-117
Identity = 221/248 (89.11%), Postives = 235/248 (94.76%), Query Frame = 0


Query: 1   MASASDSRSSGVSGRIKSAATIMHSENQSLLAELRKALIMMKEIGVDLERDNQSRMVKEL 60
           MAS S+S S+GVS RIKSAATIM+SENQSLLAELRK+LIMMKEIGVDLERDNQSRMVKEL
Sbjct: 1   MASTSNSNSTGVSERIKSAATIMNSENQSLLAELRKSLIMMKEIGVDLERDNQSRMVKEL 60

Query: 61  ENSVVELLGTYENCNNFSSAIQSVGNRYEPKEELTDFEKLLDNEVAKLSENSSSDLLNHS 120
           ENSVVELLGTYE C+NFSSAIQSVGN YEP+EELTDFEKLLDNEVAK+SENSSS+L NHS
Sbjct: 61  ENSVVELLGTYEKCSNFSSAIQSVGNIYEPQEELTDFEKLLDNEVAKVSENSSSNLHNHS 120

Query: 121 IIRQFREAIWNVHHAGQPMPGEEQEDIVMTSTQCNLLNVTCPLSGKPVTELEEPVRSMEC 180
           IIRQFREAIWNVHHAGQPMPGEEQED+VMTSTQCNLLNVTCPLSGKPVTEL EPVRS EC
Sbjct: 121 IIRQFREAIWNVHHAGQPMPGEEQEDVVMTSTQCNLLNVTCPLSGKPVTELAEPVRSTEC 180

Query: 181 KHIYEKAAIMQYLKSKKSRAQCPVAACPKMLQPEKVVPDPFLQIEIDELRKMSRHSARIQ 240
           KH+YEK AIMQYLKSK+SRAQCPVAACPKMLQPEKV+ DPFL IEIDELRK S+HS RIQ
Sbjct: 181 KHVYEKVAIMQYLKSKRSRAQCPVAACPKMLQPEKVISDPFLPIEIDELRKTSKHSVRIQ 240

Query: 241 DFTELDAD 249
           DFTE+DAD
Sbjct: 241 DFTEVDAD 248

[Close]

BLAST of Lsi04G020410 vs. NCBI nr
Match: XP_008463706.1 (PREDICTED: E3 SUMO-protein ligase MMS21 [Cucumis melo] >KAA0066802.1 E3 SUMO-protein ligase MMS21 [Cucumis melo var. makuwa] >TYK27949.1 E3 SUMO-protein ligase MMS21 [Cucumis melo var. makuwa])

HSP 1 Score: 430.3 bits (1105), Expect = 1.2e-116
Identity = 220/248 (88.71%), Postives = 233/248 (93.95%), Query Frame = 0


Query: 1   MASASDSRSSGVSGRIKSAATIMHSENQSLLAELRKALIMMKEIGVDLERDNQSRMVKEL 60
           MASASDSRS+GV+GRIKSAATIMHSENQSLLAELRK LIMMKEIGVDLE++NQ++MVKEL
Sbjct: 1   MASASDSRSTGVTGRIKSAATIMHSENQSLLAELRKTLIMMKEIGVDLEKENQNKMVKEL 60

Query: 61  ENSVVELLGTYENCNNFSSAIQSVGNRYEPKEELTDFEKLLDNEVAKLSENSSSDLLNHS 120
           E S+VELL  YENCNNFSSAIQSVGN YEPKEELTDFEKLLD+EVAK+S NSSS+  NH 
Sbjct: 61  EKSMVELLSAYENCNNFSSAIQSVGNTYEPKEELTDFEKLLDDEVAKVSVNSSSNFANHP 120

Query: 121 IIRQFREAIWNVHHAGQPMPGEEQEDIVMTSTQCNLLNVTCPLSGKPVTELEEPVRSMEC 180
           IIRQFREAIWNVHHAGQPM GEEQED+VMTSTQCNLLNVTCPLSGKPVTEL EPVRS EC
Sbjct: 121 IIRQFREAIWNVHHAGQPMAGEEQEDVVMTSTQCNLLNVTCPLSGKPVTELAEPVRSAEC 180

Query: 181 KHIYEKAAIMQYLKSKKSRAQCPVAACPKMLQPEKVVPDPFLQIEIDELRKMSRHSARIQ 240
           KHIYEKAAIMQYL SKKSRAQCPVAACPKMLQP+KVV DPFL+IEIDELRKMSRHS RIQ
Sbjct: 181 KHIYEKAAIMQYLNSKKSRAQCPVAACPKMLQPDKVVLDPFLEIEIDELRKMSRHSERIQ 240

Query: 241 DFTELDAD 249
           DFTELDAD
Sbjct: 241 DFTELDAD 248

[Close]

BLAST of Lsi04G020410 vs. NCBI nr
Match: XP_022139610.1 (E3 SUMO-protein ligase MMS21 isoform X1 [Momordica charantia])

HSP 1 Score: 429.1 bits (1102), Expect = 2.6e-116
Identity = 217/248 (87.50%), Postives = 234/248 (94.35%), Query Frame = 0


Query: 1   MASASDSRSSGVSGRIKSAATIMHSENQSLLAELRKALIMMKEIGVDLERDNQSRMVKEL 60
           MAS SD+RSS VSGRIKSAAT+MHSENQSLLAE+RK LIMMKEIG+DLERDNQS MVK+L
Sbjct: 1   MASTSDTRSSAVSGRIKSAATMMHSENQSLLAEIRKLLIMMKEIGMDLERDNQSTMVKKL 60

Query: 61  ENSVVELLGTYENCNNFSSAIQSVGNRYEPKEELTDFEKLLDNEVAKLSENSSSDLLNHS 120
           EN+VVELL TYENC+NFSSAIQSVGN YEP+EELTDFEKLLD+EVAK+SENSSS+L NHS
Sbjct: 61  ENAVVELLSTYENCDNFSSAIQSVGNIYEPREELTDFEKLLDDEVAKVSENSSSNLQNHS 120

Query: 121 IIRQFREAIWNVHHAGQPMPGEEQEDIVMTSTQCNLLNVTCPLSGKPVTELEEPVRSMEC 180
           IIR+FREAIWNVHHAGQPMPGEEQEDIVMTSTQCNLLNVTCPLSGKPVTEL +PVRSMEC
Sbjct: 121 IIRKFREAIWNVHHAGQPMPGEEQEDIVMTSTQCNLLNVTCPLSGKPVTELAQPVRSMEC 180

Query: 181 KHIYEKAAIMQYLKSKKSRAQCPVAACPKMLQPEKVVPDPFLQIEIDELRKMSRHSARIQ 240
           KH+YEK A+MQYL+SK SRAQCPVAACPKMLQPEKV PDPFL IEIDELRK SRHS RIQ
Sbjct: 181 KHVYEKEAVMQYLRSKNSRAQCPVAACPKMLQPEKVTPDPFLLIEIDELRKTSRHSVRIQ 240

Query: 241 DFTELDAD 249
           DFTELDA+
Sbjct: 241 DFTELDAE 248

[Close]

BLAST of Lsi04G020410 vs. TAIR 10
Match: AT3G15150.1 (RING/U-box superfamily protein )

HSP 1 Score: 276.6 bits (706), Expect = 2.0e-74
Identity = 141/244 (57.79%), Postives = 187/244 (76.64%), Query Frame = 0


Query: 4   ASDSRSSGVSGRIKSAATIMHSENQSLLAELRKALIMMKEIGVDLERDNQSRMVKELENS 63
           AS S S GV+GRI++A+ ++ S+N S LA++RKA+ MMK I V LE++NQ+  VK+LENS
Sbjct: 2   ASASSSDGVAGRIQNASLVLVSDNSSTLADIRKAVAMMKNIAVQLEKENQTDKVKDLENS 61

Query: 64  VVELLGTYENCNNFSSAIQSVGNRYEPKEELTDFEKLLDNEVAKLSENSSSDLLNHSIIR 123
           V ELL  + +CN+ S+AIQSV NRY+P E+LTDF+KLLD+E  KL    SS   N  ++R
Sbjct: 62  VAELLDLHSDCNHRSTAIQSVANRYQPVEQLTDFKKLLDDEFTKLKATPSSVPQNDHLMR 121

Query: 124 QFREAIWNVHHAGQPMPGEEQEDIVMTSTQCNLLNVTCPLSGKPVTELEEPVRSMECKHI 183
           QFREA+WNVHHAG+PMPG++ EDIVMTSTQC LLN+TCPLSGKPVTEL +PVRSM+C+H+
Sbjct: 122 QFREAVWNVHHAGEPMPGDDDEDIVMTSTQCPLLNMTCPLSGKPVTELADPVRSMDCRHV 181

Query: 184 YEKAAIMQYLKSKKSRAQCPVAACPKMLQPEKVVPDPFLQIEIDELRKMSRHSAR---IQ 243
           YEK+ I+ Y+ +  + A CPVA C   LQ  KV+ D  L+ EI+E+R +++ S R   I+
Sbjct: 182 YEKSVILHYIVNNPN-ANCPVAGCRGKLQNSKVICDAMLKFEIEEMRSLNKQSNRAEVIE 241

Query: 244 DFTE 245
           DFTE
Sbjct: 242 DFTE 244

The following BLAST results are available for this feature:

BLAST of Lsi04G020410 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8GYH7	2.8e-73	57.79	E3 SUMO-protein ligase MMS21 OS=Arabidopsis thaliana OX=3702 GN=MMS21 PE=1 SV=1	[more]

BLAST of Lsi04G020410 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5D3DWX9	5.6e-117	88.71	E3 SUMO-protein ligase MMS21 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sca...	[more]
A0A1S3CLF9	5.6e-117	88.71	E3 SUMO-protein ligase MMS21 OS=Cucumis melo OX=3656 GN=LOC103501788 PE=3 SV=1	[more]
A0A6J1CCS6	1.3e-116	87.50	E3 SUMO-protein ligase MMS21 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111...	[more]
A0A6J1FRP6	1.3e-116	88.31	E3 SUMO-protein ligase MMS21 OS=Cucurbita moschata OX=3662 GN=LOC111446631 PE=3 ...	[more]
A0A6J1IXZ2	8.2e-116	88.62	E3 SUMO-protein ligase MMS21 OS=Cucurbita maxima OX=3661 GN=LOC111480943 PE=3 SV...	[more]

BLAST of Lsi04G020410 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038897059.1	6.6e-120	91.13	E3 SUMO-protein ligase MMS21 isoform X1 [Benincasa hispida]	[more]
XP_023525588.1	2.3e-117	89.11	E3 SUMO-protein ligase MMS21 [Cucurbita pepo subsp. pepo] >KAG7037426.1 E3 SUMO-...	[more]
KAG6607898.1	3.1e-117	89.11	E3 SUMO-protein ligase MMS21, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_008463706.1	1.2e-116	88.71	PREDICTED: E3 SUMO-protein ligase MMS21 [Cucumis melo] >KAA0066802.1 E3 SUMO-pro...	[more]
XP_022139610.1	2.6e-116	87.50	E3 SUMO-protein ligase MMS21 isoform X1 [Momordica charantia]	[more]

BLAST of Lsi04G020410 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G15150.1	2.0e-74	57.79	RING/U-box superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR004181	Zinc finger, MIZ-type	PFAM	PF11789	zf-Nse	coord: 145..207 e-value: 5.2E-15 score: 55.1
IPR004181	Zinc finger, MIZ-type	CDD	cd16651	SPL-RING_NSE2	coord: 160..228 e-value: 4.14578E-26 score: 95.004
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 153..241 e-value: 3.2E-20 score: 74.1
IPR026846	E3 SUMO-protein ligase Nse2 (Mms21)	PANTHER	PTHR21330	UNCHARACTERIZED	coord: 8..236
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 159..231

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi04G020410.1	Lsi04G020410.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0000724	double-strand break repair via homologous recombination
biological_process	GO:0060250	germ-line stem-cell niche homeostasis
biological_process	GO:0032876	negative regulation of DNA endoreduplication
biological_process	GO:0008284	positive regulation of cell population proliferation
biological_process	GO:0080038	positive regulation of cytokinin-activated signaling pathway
biological_process	GO:0045931	positive regulation of mitotic cell cycle
biological_process	GO:0016925	protein sumoylation
biological_process	GO:0048509	regulation of meristem development
biological_process	GO:0010082	regulation of root meristem growth
cellular_component	GO:0005634	nucleus
cellular_component	GO:0030915	Smc5-Smc6 complex
molecular_function	GO:0016874	ligase activity
molecular_function	GO:0061665	SUMO ligase activity
molecular_function	GO:0008270	zinc ion binding
molecular_function	GO:0019789	SUMO transferase activity