Lsi04G018280 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi04G018280
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionGlyceraldehyde-3-phosphate dehydrogenase
Locationchr04: 25431480 .. 25432784 (-)
RNA-Seq ExpressionLsi04G018280
SyntenyLsi04G018280
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGGTTGAACTACGCAGAGCATGTGTTTGGAAAGCTCCCACATTATATCCCTATCCACAAAATGCTTGGCGAAAAAGCCAAATTGAAATTTGGAAAATAGAGTTCAGATAAATTGTTTATAAAAATACTTACGTGGCTCCCGAGAAACACTTGCCGTATCCCTTCCATCAAATTATCTTATTACAATGTTGGAAATTATTTTTATTTATTTTAATATTTTAATTATTTTTTTAGTGTGCGTTATGGAAGGGAAAGTATAGAGATACAGTATTGTTAGCCAGTTGTTTTTTCCTTTTTGTTTTTTAGTGATTTTTTCTTTTTCCTCTTCATTTTTTTTTTCAAATGATTAAAATGTTCTTCAAAGGAATAGCAAATTGAAAATAAAATAGAATCAACGTTAAATGACTCAAATAACTCACTACTAAGGATATTATTTGTTCTCTTCTAAATTCAATGATATAAAATTCTTATCAAAATTGTTAAACCAGTCCCTAATAATTTATCCTTAGTAGTGAGCTCTTCTAAATTCAATGATATAAAATTCTTATCAAAATTGTTAAACCAGTCCCAAATAATTTATCTTTAGTAGTGAGCTCTTCTAAATTCAATGATATAAAATTCTTATCAAAATTGTTGAACCAATCTCAAACTCTTCCAACCCCAAGAATCTCATAACCTCTTGTCATGTTTTTGGGATTATTATTTAGAGACTTTTGGATGCATCATACCGTGACTTGAGGCGAGCGAGGGCTGCAACTTTGAACATTGTCCCTATGAGCATGGGTGCAGCAAAGACTGTGTCCCTTGTGCTTCCCCAACTTAAGGGCAAGCTCAATAGTATTGCACTTCATGTGCCTACTCCAAACGTTTCAGTTGTCGATCTCGTTGTGAACGTTGAGAAGAAAGGCATCTCCCCCGACGACGTCAATGTTGCCTTTAGAAAGGTAGCTGATGGACCATTGAAGGGCGTTTTAGCTGTGTGCGATATCCCTCTTGTTTCTGTAGACTTCAAGTATACTGATGTTTCCTCCACGATTGACTCCTCATTAACAATAGTAATGGGAGATGACATGCTCAAGATCGTTGCCTGGTACGGCAATAAATAGGGATACAGGTGAGGACTTTCATTCATAGAAAATGATGTTCCGCTTAACATTATAAACCAAACCACATTAATTTTCATTTGCTTATTTCTGTCTGCAGCCAAAGAGTTGTCGACTTGACTCATTTGGTGGCAAACAAATAACTAGGGGCAAGATCGGGAAAAAGCAGAGATTCATTGGAAGACTTCTGTCAGACTAA

mRNA sequence

ATGGCGAGACTTTTGGATGCATCATACCGTGACTTGAGGCGAGCGAGGGCTGCAACTTTGAACATTGTCCCTATGAGCATGGGTGCAGCAAAGACTGTGTCCCTTGTGCTTCCCCAACTTAAGGGCAAGCTCAATAGTATTGCACTTCATGTGCCTACTCCAAACGTTTCAGTTGTCGATCTCGTTGTGAACGTTGAGAAGAAAGGCATCTCCCCCGACGACGTCAATGTTGCCTTTAGAAAGGTAGCTGATGGACCATTGAAGGGCGTTTTAGCTGTGTGCGATATCCCTCTTGTTTCTGTAGACTTCAAGTATACTGATGTTTCCTCCACGATTGACTCCTCATTAACAATAGTAATGGGAGATGACATGCTCAAGATCCCAAAGAGTTGTCGACTTGACTCATTTGGTGGCAAACAAATAACTAGGGGCAAGATCGGGAAAAAGCAGAGATTCATTGGAAGACTTCTGTCAGACTAA

Coding sequence (CDS)

ATGGCGAGACTTTTGGATGCATCATACCGTGACTTGAGGCGAGCGAGGGCTGCAACTTTGAACATTGTCCCTATGAGCATGGGTGCAGCAAAGACTGTGTCCCTTGTGCTTCCCCAACTTAAGGGCAAGCTCAATAGTATTGCACTTCATGTGCCTACTCCAAACGTTTCAGTTGTCGATCTCGTTGTGAACGTTGAGAAGAAAGGCATCTCCCCCGACGACGTCAATGTTGCCTTTAGAAAGGTAGCTGATGGACCATTGAAGGGCGTTTTAGCTGTGTGCGATATCCCTCTTGTTTCTGTAGACTTCAAGTATACTGATGTTTCCTCCACGATTGACTCCTCATTAACAATAGTAATGGGAGATGACATGCTCAAGATCCCAAAGAGTTGTCGACTTGACTCATTTGGTGGCAAACAAATAACTAGGGGCAAGATCGGGAAAAAGCAGAGATTCATTGGAAGACTTCTGTCAGACTAA

Protein sequence

MARLLDASYRDLRRARAATLNIVPMSMGAAKTVSLVLPQLKGKLNSIALHVPTPNVSVVDLVVNVEKKGISPDDVNVAFRKVADGPLKGVLAVCDIPLVSVDFKYTDVSSTIDSSLTIVMGDDMLKIPKSCRLDSFGGKQITRGKIGKKQRFIGRLLSD
Homology
BLAST of Lsi04G018280 vs. ExPASy Swiss-Prot
Match: P09044 (Glyceraldehyde-3-phosphate dehydrogenase B, chloroplastic (Fragment) OS=Nicotiana tabacum OX=4097 GN=GAPB PE=2 SV=1)

HSP 1 Score: 198.7 bits (504), Expect = 4.8e-50
Identity = 105/125 (84.00%), Postives = 113/125 (90.40%), Query Frame = 0

Query: 3   RLLDASYRDLRRARAATLNIVPMSMGAAKTVSLVLPQLKGKLNSIALHVPTPNVSVVDLV 62
           RLLDAS+RDLRRARAA LNIVP S GAAK VSLVLPQLKGKLN IAL VPTPNVSVVDLV
Sbjct: 242 RLLDASHRDLRRARAAALNIVPTSTGAAKAVSLVLPQLKGKLNGIALRVPTPNVSVVDLV 301

Query: 63  VNVEKKGISPDDVNVAFRKVADGPLKGVLAVCDIPLVSVDFKYTDVSSTIDSSLTIVMGD 122
           VNV KKGI+ +DVN AFRK ADGPLKGVLAVCD PLVSVDF+ +DVSSTIDSSLT+VMGD
Sbjct: 302 VNVAKKGITAEDVNAAFRKAADGPLKGVLAVCDEPLVSVDFRCSDVSSTIDSSLTMVMGD 361

Query: 123 DMLKI 128
           DM+K+
Sbjct: 362 DMVKV 366

BLAST of Lsi04G018280 vs. ExPASy Swiss-Prot
Match: P12859 (Glyceraldehyde-3-phosphate dehydrogenase B, chloroplastic OS=Pisum sativum OX=3888 GN=GAPB PE=1 SV=2)

HSP 1 Score: 196.8 bits (499), Expect = 1.8e-49
Identity = 102/125 (81.60%), Postives = 113/125 (90.40%), Query Frame = 0

Query: 3   RLLDASYRDLRRARAATLNIVPMSMGAAKTVSLVLPQLKGKLNSIALHVPTPNVSVVDLV 62
           RLLDAS+RDLRRARAA LNIVP S GAAK VSLVLPQLKGKLN IAL VPTPNVSVVDLV
Sbjct: 273 RLLDASHRDLRRARAAALNIVPTSTGAAKAVSLVLPQLKGKLNGIALRVPTPNVSVVDLV 332

Query: 63  VNVEKKGISPDDVNVAFRKVADGPLKGVLAVCDIPLVSVDFKYTDVSSTIDSSLTIVMGD 122
           VNV KKGIS +DVN AFRK A+GPLKG+L VCD+PLVSVDF+ +DVS+TIDSSLT+VMGD
Sbjct: 333 VNVAKKGISAEDVNAAFRKAAEGPLKGILDVCDVPLVSVDFRCSDVSTTIDSSLTMVMGD 392

Query: 123 DMLKI 128
           DM+K+
Sbjct: 393 DMVKV 397

BLAST of Lsi04G018280 vs. ExPASy Swiss-Prot
Match: P25857 (Glyceraldehyde-3-phosphate dehydrogenase GAPB, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=GAPB PE=1 SV=2)

HSP 1 Score: 193.4 bits (490), Expect = 2.0e-48
Identity = 99/125 (79.20%), Postives = 113/125 (90.40%), Query Frame = 0

Query: 3   RLLDASYRDLRRARAATLNIVPMSMGAAKTVSLVLPQLKGKLNSIALHVPTPNVSVVDLV 62
           RLLDAS+RDLRRARAA LNIVP S GAAK VSLVLPQLKGKLN IAL VPTPNVSVVDLV
Sbjct: 269 RLLDASHRDLRRARAAALNIVPTSTGAAKAVSLVLPQLKGKLNGIALRVPTPNVSVVDLV 328

Query: 63  VNVEKKGISPDDVNVAFRKVADGPLKGVLAVCDIPLVSVDFKYTDVSSTIDSSLTIVMGD 122
           +NVEKKG++ +DVN AFRK A+GP+KG+L VCD PLVSVDF+ +DVS+TIDSSLT+VMGD
Sbjct: 329 INVEKKGLTAEDVNEAFRKAANGPMKGILDVCDAPLVSVDFRCSDVSTTIDSSLTMVMGD 388

Query: 123 DMLKI 128
           DM+K+
Sbjct: 389 DMVKV 393

BLAST of Lsi04G018280 vs. ExPASy Swiss-Prot
Match: P12860 (Glyceraldehyde-3-phosphate dehydrogenase B, chloroplastic OS=Spinacia oleracea OX=3562 GN=GAPB PE=1 SV=1)

HSP 1 Score: 189.1 bits (479), Expect = 3.8e-47
Identity = 100/125 (80.00%), Postives = 110/125 (88.00%), Query Frame = 0

Query: 3   RLLDASYRDLRRARAATLNIVPMSMGAAKTVSLVLPQLKGKLNSIALHVPTPNVSVVDLV 62
           RLLDAS+RDLRRARAA LNIVP S GAAK VSLVLPQLKGKLN IAL VPTPNVSVVDLV
Sbjct: 272 RLLDASHRDLRRARAAALNIVPTSTGAAKAVSLVLPQLKGKLNGIALRVPTPNVSVVDLV 331

Query: 63  VNVEKKGISPDDVNVAFRKVADGPLKGVLAVCDIPLVSVDFKYTDVSSTIDSSLTIVMGD 122
           VN+EK G++ +DVN AFRK A GPLKGVL VCDIPLVSVDF+ +D SSTIDSSLT+VMG 
Sbjct: 332 VNIEKVGVTAEDVNNAFRKAAAGPLKGVLDVCDIPLVSVDFRCSDFSSTIDSSLTMVMGG 391

Query: 123 DMLKI 128
           DM+K+
Sbjct: 392 DMVKV 396

BLAST of Lsi04G018280 vs. ExPASy Swiss-Prot
Match: P19866 (Glyceraldehyde-3-phosphate dehydrogenase A, chloroplastic OS=Spinacia oleracea OX=3562 GN=GAPA PE=1 SV=2)

HSP 1 Score: 175.3 bits (443), Expect = 5.7e-43
Identity = 94/125 (75.20%), Postives = 107/125 (85.60%), Query Frame = 0

Query: 3   RLLDASYRDLRRARAATLNIVPMSMGAAKTVSLVLPQLKGKLNSIALHVPTPNVSVVDLV 62
           RLLDAS+RDLRRARAA LNIVP S GAAK V+LVLP LKGKLN IAL VPTPNVSVVDLV
Sbjct: 252 RLLDASHRDLRRARAACLNIVPTSTGAAKAVALVLPNLKGKLNGIALRVPTPNVSVVDLV 311

Query: 63  VNVEKKGISPDDVNVAFRKVADGPLKGVLAVCDIPLVSVDFKYTDVSSTIDSSLTIVMGD 122
           V V KK  + ++VN AFR+ AD  LKG+L+VCD PLVS+DF+ TDVSSTIDSSLT+VMGD
Sbjct: 312 VQVSKKTFA-EEVNAAFRESADNELKGILSVCDEPLVSIDFRCTDVSSTIDSSLTMVMGD 371

Query: 123 DMLKI 128
           DM+K+
Sbjct: 372 DMVKV 375

BLAST of Lsi04G018280 vs. ExPASy TrEMBL
Match: A0A5D3D8Q8 (Glyceraldehyde-3-phosphate dehydrogenase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold134G00460 PE=3 SV=1)

HSP 1 Score: 209.9 bits (533), Expect = 7.7e-51
Identity = 111/125 (88.80%), Postives = 115/125 (92.00%), Query Frame = 0

Query: 3   RLLDASYRDLRRARAATLNIVPMSMGAAKTVSLVLPQLKGKLNSIALHVPTPNVSVVDLV 62
           RLLDAS+RDLRRARAA LNIVP S GAAK VSLVLPQLKGKLN IAL VPTPNVSVVDLV
Sbjct: 272 RLLDASHRDLRRARAAALNIVPTSTGAAKAVSLVLPQLKGKLNGIALRVPTPNVSVVDLV 331

Query: 63  VNVEKKGISPDDVNVAFRKVADGPLKGVLAVCDIPLVSVDFKYTDVSSTIDSSLTIVMGD 122
           VN+EKKGIS DDVN AFRK ADGPLKGVLAVCDIPLVSVDFK TDVSSTIDSSLT+VMGD
Sbjct: 332 VNIEKKGISADDVNAAFRKAADGPLKGVLAVCDIPLVSVDFKCTDVSSTIDSSLTMVMGD 391

Query: 123 DMLKI 128
           DMLK+
Sbjct: 392 DMLKV 396

BLAST of Lsi04G018280 vs. ExPASy TrEMBL
Match: A0A6J1IDR4 (Glyceraldehyde-3-phosphate dehydrogenase OS=Cucurbita maxima OX=3661 GN=LOC111474421 PE=3 SV=1)

HSP 1 Score: 209.9 bits (533), Expect = 7.7e-51
Identity = 111/125 (88.80%), Postives = 115/125 (92.00%), Query Frame = 0

Query: 3   RLLDASYRDLRRARAATLNIVPMSMGAAKTVSLVLPQLKGKLNSIALHVPTPNVSVVDLV 62
           RLLDAS+RDLRRARAA LNIVP S GAAK VSLVLPQLKGKLN IAL VPTPNVSVVDLV
Sbjct: 273 RLLDASHRDLRRARAAALNIVPTSTGAAKAVSLVLPQLKGKLNGIALRVPTPNVSVVDLV 332

Query: 63  VNVEKKGISPDDVNVAFRKVADGPLKGVLAVCDIPLVSVDFKYTDVSSTIDSSLTIVMGD 122
           VN+EKKGIS DDVN AFRK ADGPLKGVLAVCDIPLVSVDFK TDVSSTIDSSLT+VMGD
Sbjct: 333 VNIEKKGISADDVNAAFRKAADGPLKGVLAVCDIPLVSVDFKCTDVSSTIDSSLTMVMGD 392

Query: 123 DMLKI 128
           DMLK+
Sbjct: 393 DMLKV 397

BLAST of Lsi04G018280 vs. ExPASy TrEMBL
Match: A0A6J1ICN9 (Glyceraldehyde-3-phosphate dehydrogenase OS=Cucurbita maxima OX=3661 GN=LOC111474421 PE=3 SV=1)

HSP 1 Score: 209.9 bits (533), Expect = 7.7e-51
Identity = 111/125 (88.80%), Postives = 115/125 (92.00%), Query Frame = 0

Query: 3   RLLDASYRDLRRARAATLNIVPMSMGAAKTVSLVLPQLKGKLNSIALHVPTPNVSVVDLV 62
           RLLDAS+RDLRRARAA LNIVP S GAAK VSLVLPQLKGKLN IAL VPTPNVSVVDLV
Sbjct: 272 RLLDASHRDLRRARAAALNIVPTSTGAAKAVSLVLPQLKGKLNGIALRVPTPNVSVVDLV 331

Query: 63  VNVEKKGISPDDVNVAFRKVADGPLKGVLAVCDIPLVSVDFKYTDVSSTIDSSLTIVMGD 122
           VN+EKKGIS DDVN AFRK ADGPLKGVLAVCDIPLVSVDFK TDVSSTIDSSLT+VMGD
Sbjct: 332 VNIEKKGISADDVNAAFRKAADGPLKGVLAVCDIPLVSVDFKCTDVSSTIDSSLTMVMGD 391

Query: 123 DMLKI 128
           DMLK+
Sbjct: 392 DMLKV 396

BLAST of Lsi04G018280 vs. ExPASy TrEMBL
Match: A0A1M4NEC6 (Glyceraldehyde-3-phosphate dehydrogenase OS=Cucumis sativus OX=3659 GN=GAP B PE=2 SV=1)

HSP 1 Score: 209.9 bits (533), Expect = 7.7e-51
Identity = 111/125 (88.80%), Postives = 115/125 (92.00%), Query Frame = 0

Query: 3   RLLDASYRDLRRARAATLNIVPMSMGAAKTVSLVLPQLKGKLNSIALHVPTPNVSVVDLV 62
           RLLDAS+RDLRRARAA LNIVP S GAAK VSLVLPQLKGKLN IAL VPTPNVSVVDLV
Sbjct: 273 RLLDASHRDLRRARAAALNIVPTSTGAAKAVSLVLPQLKGKLNGIALRVPTPNVSVVDLV 332

Query: 63  VNVEKKGISPDDVNVAFRKVADGPLKGVLAVCDIPLVSVDFKYTDVSSTIDSSLTIVMGD 122
           VN+EKKGIS DDVN AFRK ADGPLKGVLAVCDIPLVSVDFK TDVSSTIDSSLT+VMGD
Sbjct: 333 VNIEKKGISADDVNAAFRKAADGPLKGVLAVCDIPLVSVDFKCTDVSSTIDSSLTMVMGD 392

Query: 123 DMLKI 128
           DMLK+
Sbjct: 393 DMLKV 397

BLAST of Lsi04G018280 vs. ExPASy TrEMBL
Match: A0A1B5FYN9 (Glyceraldehyde-3-phosphate dehydrogenase OS=Cucumis sativus OX=3659 GN=GAPDH B PE=2 SV=1)

HSP 1 Score: 209.9 bits (533), Expect = 7.7e-51
Identity = 111/125 (88.80%), Postives = 115/125 (92.00%), Query Frame = 0

Query: 3   RLLDASYRDLRRARAATLNIVPMSMGAAKTVSLVLPQLKGKLNSIALHVPTPNVSVVDLV 62
           RLLDAS+RDLRRARAA LNIVP S GAAK VSLVLPQLKGKLN IAL VPTPNVSVVDLV
Sbjct: 272 RLLDASHRDLRRARAAALNIVPTSTGAAKAVSLVLPQLKGKLNGIALRVPTPNVSVVDLV 331

Query: 63  VNVEKKGISPDDVNVAFRKVADGPLKGVLAVCDIPLVSVDFKYTDVSSTIDSSLTIVMGD 122
           VN+EKKGIS DDVN AFRK ADGPLKGVLAVCDIPLVSVDFK TDVSSTIDSSLT+VMGD
Sbjct: 332 VNIEKKGISADDVNAAFRKAADGPLKGVLAVCDIPLVSVDFKCTDVSSTIDSSLTMVMGD 391

Query: 123 DMLKI 128
           DMLK+
Sbjct: 392 DMLKV 396

BLAST of Lsi04G018280 vs. NCBI nr
Match: XP_022936062.1 (glyceraldehyde-3-phosphate dehydrogenase B, chloroplastic isoform X2 [Cucurbita moschata] >KAG7024091.1 Glyceraldehyde-3-phosphate dehydrogenase B, chloroplastic [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 209.9 bits (533), Expect = 1.6e-50
Identity = 111/125 (88.80%), Postives = 115/125 (92.00%), Query Frame = 0

Query: 3   RLLDASYRDLRRARAATLNIVPMSMGAAKTVSLVLPQLKGKLNSIALHVPTPNVSVVDLV 62
           RLLDAS+RDLRRARAA LNIVP S GAAK VSLVLPQLKGKLN IAL VPTPNVSVVDLV
Sbjct: 272 RLLDASHRDLRRARAAALNIVPTSTGAAKAVSLVLPQLKGKLNGIALRVPTPNVSVVDLV 331

Query: 63  VNVEKKGISPDDVNVAFRKVADGPLKGVLAVCDIPLVSVDFKYTDVSSTIDSSLTIVMGD 122
           VN+EKKGIS DDVN AFRK ADGPLKGVLAVCDIPLVSVDFK TDVSSTIDSSLT+VMGD
Sbjct: 332 VNIEKKGISADDVNAAFRKAADGPLKGVLAVCDIPLVSVDFKCTDVSSTIDSSLTMVMGD 391

Query: 123 DMLKI 128
           DMLK+
Sbjct: 392 DMLKV 396

BLAST of Lsi04G018280 vs. NCBI nr
Match: QFX66114.1 (chloroplastic glyceraldehyde-3-phosphate dehydrogenase B [Luffa aegyptiaca])

HSP 1 Score: 209.9 bits (533), Expect = 1.6e-50
Identity = 111/125 (88.80%), Postives = 115/125 (92.00%), Query Frame = 0

Query: 3   RLLDASYRDLRRARAATLNIVPMSMGAAKTVSLVLPQLKGKLNSIALHVPTPNVSVVDLV 62
           RLLDAS+RDLRRARAA LNIVP S GAAK VSLVLPQLKGKLN IAL VPTPNVSVVDLV
Sbjct: 272 RLLDASHRDLRRARAAALNIVPTSTGAAKAVSLVLPQLKGKLNGIALRVPTPNVSVVDLV 331

Query: 63  VNVEKKGISPDDVNVAFRKVADGPLKGVLAVCDIPLVSVDFKYTDVSSTIDSSLTIVMGD 122
           VN+EKKGIS DDVN AFRK ADGPLKGVLAVCDIPLVSVDFK TDVSSTIDSSLT+VMGD
Sbjct: 332 VNIEKKGISADDVNAAFRKAADGPLKGVLAVCDIPLVSVDFKCTDVSSTIDSSLTMVMGD 391

Query: 123 DMLKI 128
           DMLK+
Sbjct: 392 DMLKV 396

BLAST of Lsi04G018280 vs. NCBI nr
Match: AAG23800.1 (chloroplast NADP-dependent glyceraldehyde 3-phosphate dehydrogenase B subunit, partial [Cucurbita pepo])

HSP 1 Score: 209.9 bits (533), Expect = 1.6e-50
Identity = 111/125 (88.80%), Postives = 115/125 (92.00%), Query Frame = 0

Query: 3   RLLDASYRDLRRARAATLNIVPMSMGAAKTVSLVLPQLKGKLNSIALHVPTPNVSVVDLV 62
           RLLDAS+RDLRRARAA LNIVP S GAAK VSLVLPQLKGKLN IAL VPTPNVSVVDLV
Sbjct: 38  RLLDASHRDLRRARAAALNIVPTSTGAAKAVSLVLPQLKGKLNGIALRVPTPNVSVVDLV 97

Query: 63  VNVEKKGISPDDVNVAFRKVADGPLKGVLAVCDIPLVSVDFKYTDVSSTIDSSLTIVMGD 122
           VN+EKKGIS DDVN AFRK ADGPLKGVLAVCDIPLVSVDFK TDVSSTIDSSLT+VMGD
Sbjct: 98  VNIEKKGISADDVNAAFRKAADGPLKGVLAVCDIPLVSVDFKCTDVSSTIDSSLTMVMGD 157

Query: 123 DMLKI 128
           DMLK+
Sbjct: 158 DMLKV 162

BLAST of Lsi04G018280 vs. NCBI nr
Match: CZF77070.1 (Glyceraldehyde phosphate dehydrogenase B [Cucumis sativus])

HSP 1 Score: 209.9 bits (533), Expect = 1.6e-50
Identity = 111/125 (88.80%), Postives = 115/125 (92.00%), Query Frame = 0

Query: 3   RLLDASYRDLRRARAATLNIVPMSMGAAKTVSLVLPQLKGKLNSIALHVPTPNVSVVDLV 62
           RLLDAS+RDLRRARAA LNIVP S GAAK VSLVLPQLKGKLN IAL VPTPNVSVVDLV
Sbjct: 272 RLLDASHRDLRRARAAALNIVPTSTGAAKAVSLVLPQLKGKLNGIALRVPTPNVSVVDLV 331

Query: 63  VNVEKKGISPDDVNVAFRKVADGPLKGVLAVCDIPLVSVDFKYTDVSSTIDSSLTIVMGD 122
           VN+EKKGIS DDVN AFRK ADGPLKGVLAVCDIPLVSVDFK TDVSSTIDSSLT+VMGD
Sbjct: 332 VNIEKKGISADDVNAAFRKAADGPLKGVLAVCDIPLVSVDFKCTDVSSTIDSSLTMVMGD 391

Query: 123 DMLKI 128
           DMLK+
Sbjct: 392 DMLKV 396

BLAST of Lsi04G018280 vs. NCBI nr
Match: XP_008452998.1 (PREDICTED: glyceraldehyde-3-phosphate dehydrogenase B, chloroplastic [Cucumis melo] >KAA0064657.1 glyceraldehyde-3-phosphate dehydrogenase B [Cucumis melo var. makuwa] >TYK19934.1 glyceraldehyde-3-phosphate dehydrogenase B [Cucumis melo var. makuwa])

HSP 1 Score: 209.9 bits (533), Expect = 1.6e-50
Identity = 111/125 (88.80%), Postives = 115/125 (92.00%), Query Frame = 0

Query: 3   RLLDASYRDLRRARAATLNIVPMSMGAAKTVSLVLPQLKGKLNSIALHVPTPNVSVVDLV 62
           RLLDAS+RDLRRARAA LNIVP S GAAK VSLVLPQLKGKLN IAL VPTPNVSVVDLV
Sbjct: 272 RLLDASHRDLRRARAAALNIVPTSTGAAKAVSLVLPQLKGKLNGIALRVPTPNVSVVDLV 331

Query: 63  VNVEKKGISPDDVNVAFRKVADGPLKGVLAVCDIPLVSVDFKYTDVSSTIDSSLTIVMGD 122
           VN+EKKGIS DDVN AFRK ADGPLKGVLAVCDIPLVSVDFK TDVSSTIDSSLT+VMGD
Sbjct: 332 VNIEKKGISADDVNAAFRKAADGPLKGVLAVCDIPLVSVDFKCTDVSSTIDSSLTMVMGD 391

Query: 123 DMLKI 128
           DMLK+
Sbjct: 392 DMLKV 396

BLAST of Lsi04G018280 vs. TAIR 10
Match: AT1G42970.1 (glyceraldehyde-3-phosphate dehydrogenase B subunit )

HSP 1 Score: 193.4 bits (490), Expect = 1.4e-49
Identity = 99/125 (79.20%), Postives = 113/125 (90.40%), Query Frame = 0

Query: 3   RLLDASYRDLRRARAATLNIVPMSMGAAKTVSLVLPQLKGKLNSIALHVPTPNVSVVDLV 62
           RLLDAS+RDLRRARAA LNIVP S GAAK VSLVLPQLKGKLN IAL VPTPNVSVVDLV
Sbjct: 269 RLLDASHRDLRRARAAALNIVPTSTGAAKAVSLVLPQLKGKLNGIALRVPTPNVSVVDLV 328

Query: 63  VNVEKKGISPDDVNVAFRKVADGPLKGVLAVCDIPLVSVDFKYTDVSSTIDSSLTIVMGD 122
           +NVEKKG++ +DVN AFRK A+GP+KG+L VCD PLVSVDF+ +DVS+TIDSSLT+VMGD
Sbjct: 329 INVEKKGLTAEDVNEAFRKAANGPMKGILDVCDAPLVSVDFRCSDVSTTIDSSLTMVMGD 388

Query: 123 DMLKI 128
           DM+K+
Sbjct: 389 DMVKV 393

BLAST of Lsi04G018280 vs. TAIR 10
Match: AT1G12900.1 (glyceraldehyde 3-phosphate dehydrogenase A subunit 2 )

HSP 1 Score: 171.0 bits (432), Expect = 7.6e-43
Identity = 93/125 (74.40%), Postives = 105/125 (84.00%), Query Frame = 0

Query: 3   RLLDASYRDLRRARAATLNIVPMSMGAAKTVSLVLPQLKGKLNSIALHVPTPNVSVVDLV 62
           RLLDAS+RDLRRARAA LNIVP S GAAK V+LVLP LKGKLN IAL VPTPNVSVVDLV
Sbjct: 250 RLLDASHRDLRRARAAALNIVPTSTGAAKAVALVLPNLKGKLNGIALRVPTPNVSVVDLV 309

Query: 63  VNVEKKGISPDDVNVAFRKVADGPLKGVLAVCDIPLVSVDFKYTDVSSTIDSSLTIVMGD 122
           V V KK  + ++VN AFR  A+  LKG+L VCD PLVSVDF+ +DVSSTIDSSLT+VMGD
Sbjct: 310 VQVSKKTFA-EEVNAAFRDAAEKELKGILDVCDEPLVSVDFRCSDVSSTIDSSLTMVMGD 369

Query: 123 DMLKI 128
           DM+K+
Sbjct: 370 DMVKV 373

BLAST of Lsi04G018280 vs. TAIR 10
Match: AT1G12900.2 (glyceraldehyde 3-phosphate dehydrogenase A subunit 2 )

HSP 1 Score: 171.0 bits (432), Expect = 7.6e-43
Identity = 93/125 (74.40%), Postives = 105/125 (84.00%), Query Frame = 0

Query: 3   RLLDASYRDLRRARAATLNIVPMSMGAAKTVSLVLPQLKGKLNSIALHVPTPNVSVVDLV 62
           RLLDAS+RDLRRARAA LNIVP S GAAK V+LVLP LKGKLN IAL VPTPNVSVVDLV
Sbjct: 168 RLLDASHRDLRRARAAALNIVPTSTGAAKAVALVLPNLKGKLNGIALRVPTPNVSVVDLV 227

Query: 63  VNVEKKGISPDDVNVAFRKVADGPLKGVLAVCDIPLVSVDFKYTDVSSTIDSSLTIVMGD 122
           V V KK  + ++VN AFR  A+  LKG+L VCD PLVSVDF+ +DVSSTIDSSLT+VMGD
Sbjct: 228 VQVSKKTFA-EEVNAAFRDAAEKELKGILDVCDEPLVSVDFRCSDVSSTIDSSLTMVMGD 287

Query: 123 DMLKI 128
           DM+K+
Sbjct: 288 DMVKV 291

BLAST of Lsi04G018280 vs. TAIR 10
Match: AT1G12900.3 (glyceraldehyde 3-phosphate dehydrogenase A subunit 2 )

HSP 1 Score: 171.0 bits (432), Expect = 7.6e-43
Identity = 93/125 (74.40%), Postives = 105/125 (84.00%), Query Frame = 0

Query: 3   RLLDASYRDLRRARAATLNIVPMSMGAAKTVSLVLPQLKGKLNSIALHVPTPNVSVVDLV 62
           RLLDAS+RDLRRARAA LNIVP S GAAK V+LVLP LKGKLN IAL VPTPNVSVVDLV
Sbjct: 201 RLLDASHRDLRRARAAALNIVPTSTGAAKAVALVLPNLKGKLNGIALRVPTPNVSVVDLV 260

Query: 63  VNVEKKGISPDDVNVAFRKVADGPLKGVLAVCDIPLVSVDFKYTDVSSTIDSSLTIVMGD 122
           V V KK  + ++VN AFR  A+  LKG+L VCD PLVSVDF+ +DVSSTIDSSLT+VMGD
Sbjct: 261 VQVSKKTFA-EEVNAAFRDAAEKELKGILDVCDEPLVSVDFRCSDVSSTIDSSLTMVMGD 320

Query: 123 DMLKI 128
           DM+K+
Sbjct: 321 DMVKV 324

BLAST of Lsi04G018280 vs. TAIR 10
Match: AT1G12900.4 (glyceraldehyde 3-phosphate dehydrogenase A subunit 2 )

HSP 1 Score: 171.0 bits (432), Expect = 7.6e-43
Identity = 93/125 (74.40%), Postives = 105/125 (84.00%), Query Frame = 0

Query: 3   RLLDASYRDLRRARAATLNIVPMSMGAAKTVSLVLPQLKGKLNSIALHVPTPNVSVVDLV 62
           RLLDAS+RDLRRARAA LNIVP S GAAK V+LVLP LKGKLN IAL VPTPNVSVVDLV
Sbjct: 201 RLLDASHRDLRRARAAALNIVPTSTGAAKAVALVLPNLKGKLNGIALRVPTPNVSVVDLV 260

Query: 63  VNVEKKGISPDDVNVAFRKVADGPLKGVLAVCDIPLVSVDFKYTDVSSTIDSSLTIVMGD 122
           V V KK  + ++VN AFR  A+  LKG+L VCD PLVSVDF+ +DVSSTIDSSLT+VMGD
Sbjct: 261 VQVSKKTFA-EEVNAAFRDAAEKELKGILDVCDEPLVSVDFRCSDVSSTIDSSLTMVMGD 320

Query: 123 DMLKI 128
           DM+K+
Sbjct: 321 DMVKV 324

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P090444.8e-5084.00Glyceraldehyde-3-phosphate dehydrogenase B, chloroplastic (Fragment) OS=Nicotian... [more]
P128591.8e-4981.60Glyceraldehyde-3-phosphate dehydrogenase B, chloroplastic OS=Pisum sativum OX=38... [more]
P258572.0e-4879.20Glyceraldehyde-3-phosphate dehydrogenase GAPB, chloroplastic OS=Arabidopsis thal... [more]
P128603.8e-4780.00Glyceraldehyde-3-phosphate dehydrogenase B, chloroplastic OS=Spinacia oleracea O... [more]
P198665.7e-4375.20Glyceraldehyde-3-phosphate dehydrogenase A, chloroplastic OS=Spinacia oleracea O... [more]
Match NameE-valueIdentityDescription
A0A5D3D8Q87.7e-5188.80Glyceraldehyde-3-phosphate dehydrogenase OS=Cucumis melo var. makuwa OX=1194695 ... [more]
A0A6J1IDR47.7e-5188.80Glyceraldehyde-3-phosphate dehydrogenase OS=Cucurbita maxima OX=3661 GN=LOC11147... [more]
A0A6J1ICN97.7e-5188.80Glyceraldehyde-3-phosphate dehydrogenase OS=Cucurbita maxima OX=3661 GN=LOC11147... [more]
A0A1M4NEC67.7e-5188.80Glyceraldehyde-3-phosphate dehydrogenase OS=Cucumis sativus OX=3659 GN=GAP B PE=... [more]
A0A1B5FYN97.7e-5188.80Glyceraldehyde-3-phosphate dehydrogenase OS=Cucumis sativus OX=3659 GN=GAPDH B P... [more]
Match NameE-valueIdentityDescription
XP_022936062.11.6e-5088.80glyceraldehyde-3-phosphate dehydrogenase B, chloroplastic isoform X2 [Cucurbita ... [more]
QFX66114.11.6e-5088.80chloroplastic glyceraldehyde-3-phosphate dehydrogenase B [Luffa aegyptiaca][more]
AAG23800.11.6e-5088.80chloroplast NADP-dependent glyceraldehyde 3-phosphate dehydrogenase B subunit, p... [more]
CZF77070.11.6e-5088.80Glyceraldehyde phosphate dehydrogenase B [Cucumis sativus][more]
XP_008452998.11.6e-5088.80PREDICTED: glyceraldehyde-3-phosphate dehydrogenase B, chloroplastic [Cucumis me... [more]
Match NameE-valueIdentityDescription
AT1G42970.11.4e-4979.20glyceraldehyde-3-phosphate dehydrogenase B subunit [more]
AT1G12900.17.6e-4374.40glyceraldehyde 3-phosphate dehydrogenase A subunit 2 [more]
AT1G12900.27.6e-4374.40glyceraldehyde 3-phosphate dehydrogenase A subunit 2 [more]
AT1G12900.37.6e-4374.40glyceraldehyde 3-phosphate dehydrogenase A subunit 2 [more]
AT1G12900.47.6e-4374.40glyceraldehyde 3-phosphate dehydrogenase A subunit 2 [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR020829Glyceraldehyde 3-phosphate dehydrogenase, catalytic domainPFAMPF02800Gp_dh_Ccoord: 3..127
e-value: 8.7E-39
score: 132.7
NoneNo IPR availableGENE3D3.30.360.10Dihydrodipicolinate Reductase; domain 2coord: 3..127
e-value: 6.0E-39
score: 136.0
NoneNo IPR availablePANTHERPTHR43148GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE 2coord: 3..127
NoneNo IPR availablePANTHERPTHR43148:SF5GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE GAPB, CHLOROPLASTICcoord: 3..127
NoneNo IPR availableSUPERFAMILY55347Glyceraldehyde-3-phosphate dehydrogenase-like, C-terminal domaincoord: 3..127

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi04G018280.1Lsi04G018280.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006006 glucose metabolic process
cellular_component GO:0009536 plastid
molecular_function GO:0004365 glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity
molecular_function GO:0051287 NAD binding
molecular_function GO:0050661 NADP binding
molecular_function GO:0016620 oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor