Lsi04G017760 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi04G017760
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionPentatricopeptide repeat-containing protein, mitochondrial
Locationchr04: 24980393 .. 24981997 (+)
RNA-Seq ExpressionLsi04G017760
SyntenyLsi04G017760
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTTTGATCAGATCGAAGCCTAATCTTCCACTCTTTCTATCATATCTTTCTCAGCGTATACAGAATCACCGTTCTTTCTCATCCTCCCTTTCAATTTCAGAATCTCGCCTTCAAGACGAGCCCGCGAGCGACTGTCCTCAAAATGCCACAAATCCTGTTCTCTCGCCGGAGGAAATCCAGGCTGCCGACAAATTCCACGCCCTAATCAAGGAATACTATCGAAGAAATCCCAATCCCGATCCCACTCCACCATGCCCTAATTTCACTATTTCCGCTCTTTCCAACGACCTATCCCAAATCTCAGCCCTCCATTCCGTCTCTCCAGCCGTCGTTCGTTACGTCATCGAGAAATCTGGTGGTGTCCGTCATGGCATCCCTTTTCTTCAAGCCCTCGCGTTCTTCAACTGGGCGACGGCGGGAGAGGGTTTCGAGCATTCTGCACAGCCATACAACGAGATGATTGACCTAGCCGGTAAGGTAAAACAATTCGGATTAGCGTGGTATTTGATTGATTTGATGAAAGCTAGAAATGTAGAAATTACTGTTGAAACTTTCTCAATTCTTGTGCGGCGGTATGTGAGGGCTGGATTGGCGGCGGAGGCGGTTCATGCGTTTAATCGCATGGAGGACTATGGCTGCAATCCTGACAAGATTGCTTTCTCCATTGTTATCAGCATTCTGTGCAAGAAGAGAAGGGCAATTGAAGCCCAGTCATTTTTTGATAGTCTAAAACAAAAATTCGAACCTGATGTTGTTGTGTACACTAGCTTAGTTCATGGGTGGTGTCGAGCAGGTGATATCTCAGAGGCTGAAAGAGTATTTAAAGAGATGAAGATGGCAGGGATTAGCCCTAATGTTTATACTTATAGCATTGTGATTGATGCATTGTGTAGGTCTGGTCAAATTACTCGTGCCCATGATGTTTTCTCTGAGATGATTGATTCTGGTTGCAATCCCAACTCAGTTACATTTAACAATCTTATTAGAGTTCATGTGAAAGCTGGTAGAACAGAGAAGGTGTTGCAAGTGTATAACCAAATGAAGAGATTGGGTTGTTCTCCTGACATAGTTACTTATAATTTCCTCATAGAGACTCATTGCAGGGACGACAATCTTGGAGAGGCTATAAAGATCCTCAACTCAATGTCTAAGAAAGGCTGCACACCGAATGCATCGACGTTCAACCCTATATTTAGATGTATCGCAAAGTCGCAAGATGTAAACGGTGCTCATCGGATGTTTGCTAAGATGAAGGATGTAGGTTGTAAGCCAAATACAGTGACATACAATATCTTGATGCGAATGTTTGCGGAGTCAAAATCTGCTGATATGATTTTCAAGTTGAAGAAGGAGATGGACGAGGAAGAAGTGGAGCCTAACGTGAATACATATCGAGAATTGATATCGTTATATTGCGGGATGGGGCATTGGAACAATGCATACAAGTTCTTCAGGGAAATGATTGAGGAGAAATGCTTAAAACCCAGCATGCCTCTTTATGAGATGGTTTTGCAACAACTAAGAAAGGCAGGACAGTTGAAGAAGCATGAGGAATTGGTTGATAAGATGGTTGAGAGAGGATTCGTGTCGCGGCCCCTGTAG

mRNA sequence

ATGGCTTTGATCAGATCGAAGCCTAATCTTCCACTCTTTCTATCATATCTTTCTCAGCGTATACAGAATCACCGTTCTTTCTCATCCTCCCTTTCAATTTCAGAATCTCGCCTTCAAGACGAGCCCGCGAGCGACTGTCCTCAAAATGCCACAAATCCTGTTCTCTCGCCGGAGGAAATCCAGGCTGCCGACAAATTCCACGCCCTAATCAAGGAATACTATCGAAGAAATCCCAATCCCGATCCCACTCCACCATGCCCTAATTTCACTATTTCCGCTCTTTCCAACGACCTATCCCAAATCTCAGCCCTCCATTCCGTCTCTCCAGCCGTCGTTCGTTACGTCATCGAGAAATCTGGTGGTGTCCGTCATGGCATCCCTTTTCTTCAAGCCCTCGCGTTCTTCAACTGGGCGACGGCGGGAGAGGGTTTCGAGCATTCTGCACAGCCATACAACGAGATGATTGACCTAGCCGGTAAGGTAAAACAATTCGGATTAGCGTGGTATTTGATTGATTTGATGAAAGCTAGAAATGTAGAAATTACTGTTGAAACTTTCTCAATTCTTGTGCGGCGGTATGTGAGGGCTGGATTGGCGGCGGAGGCGGTTCATGCGTTTAATCGCATGGAGGACTATGGCTGCAATCCTGACAAGATTGCTTTCTCCATTGTTATCAGCATTCTGTGCAAGAAGAGAAGGGCAATTGAAGCCCAGTCATTTTTTGATAGTCTAAAACAAAAATTCGAACCTGATGTTGTTGTGTACACTAGCTTAGTTCATGGGTGGTGTCGAGCAGGTGATATCTCAGAGGCTGAAAGAGTATTTAAAGAGATGAAGATGGCAGGGATTAGCCCTAATGTTTATACTTATAGCATTGTGATTGATGCATTGTGTAGGTCTGGTCAAATTACTCGTGCCCATGATGTTTTCTCTGAGATGATTGATTCTGGTTGCAATCCCAACTCAGTTACATTTAACAATCTTATTAGAGTTCATGTGAAAGCTGGTAGAACAGAGAAGGTGTTGCAAGTGTATAACCAAATGAAGAGATTGGGTTGTTCTCCTGACATAGTTACTTATAATTTCCTCATAGAGACTCATTGCAGGGACGACAATCTTGGAGAGGCTATAAAGATCCTCAACTCAATGTCTAAGAAAGGCTGCACACCGAATGCATCGACGTTCAACCCTATATTTAGATGTATCGCAAAGTCGCAAGATGTAAACGGTGCTCATCGGATGTTTGCTAAGATGAAGGATGTAGGTTGTAAGCCAAATACAGTGACATACAATATCTTGATGCGAATGTTTGCGGAGTCAAAATCTGCTGATATGATTTTCAAGTTGAAGAAGGAGATGGACGAGGAAGAAGTGGAGCCTAACGTGAATACATATCGAGAATTGATATCGTTATATTGCGGGATGGGGCATTGGAACAATGCATACAAGTTCTTCAGGGAAATGATTGAGGAGAAATGCTTAAAACCCAGCATGCCTCTTTATGAGATGGTTTTGCAACAACTAAGAAAGGCAGGACAGTTGAAGAAGCATGAGGAATTGGTTGATAAGATGGTTGAGAGAGGATTCGTGTCGCGGCCCCTGTAG

Coding sequence (CDS)

ATGGCTTTGATCAGATCGAAGCCTAATCTTCCACTCTTTCTATCATATCTTTCTCAGCGTATACAGAATCACCGTTCTTTCTCATCCTCCCTTTCAATTTCAGAATCTCGCCTTCAAGACGAGCCCGCGAGCGACTGTCCTCAAAATGCCACAAATCCTGTTCTCTCGCCGGAGGAAATCCAGGCTGCCGACAAATTCCACGCCCTAATCAAGGAATACTATCGAAGAAATCCCAATCCCGATCCCACTCCACCATGCCCTAATTTCACTATTTCCGCTCTTTCCAACGACCTATCCCAAATCTCAGCCCTCCATTCCGTCTCTCCAGCCGTCGTTCGTTACGTCATCGAGAAATCTGGTGGTGTCCGTCATGGCATCCCTTTTCTTCAAGCCCTCGCGTTCTTCAACTGGGCGACGGCGGGAGAGGGTTTCGAGCATTCTGCACAGCCATACAACGAGATGATTGACCTAGCCGGTAAGGTAAAACAATTCGGATTAGCGTGGTATTTGATTGATTTGATGAAAGCTAGAAATGTAGAAATTACTGTTGAAACTTTCTCAATTCTTGTGCGGCGGTATGTGAGGGCTGGATTGGCGGCGGAGGCGGTTCATGCGTTTAATCGCATGGAGGACTATGGCTGCAATCCTGACAAGATTGCTTTCTCCATTGTTATCAGCATTCTGTGCAAGAAGAGAAGGGCAATTGAAGCCCAGTCATTTTTTGATAGTCTAAAACAAAAATTCGAACCTGATGTTGTTGTGTACACTAGCTTAGTTCATGGGTGGTGTCGAGCAGGTGATATCTCAGAGGCTGAAAGAGTATTTAAAGAGATGAAGATGGCAGGGATTAGCCCTAATGTTTATACTTATAGCATTGTGATTGATGCATTGTGTAGGTCTGGTCAAATTACTCGTGCCCATGATGTTTTCTCTGAGATGATTGATTCTGGTTGCAATCCCAACTCAGTTACATTTAACAATCTTATTAGAGTTCATGTGAAAGCTGGTAGAACAGAGAAGGTGTTGCAAGTGTATAACCAAATGAAGAGATTGGGTTGTTCTCCTGACATAGTTACTTATAATTTCCTCATAGAGACTCATTGCAGGGACGACAATCTTGGAGAGGCTATAAAGATCCTCAACTCAATGTCTAAGAAAGGCTGCACACCGAATGCATCGACGTTCAACCCTATATTTAGATGTATCGCAAAGTCGCAAGATGTAAACGGTGCTCATCGGATGTTTGCTAAGATGAAGGATGTAGGTTGTAAGCCAAATACAGTGACATACAATATCTTGATGCGAATGTTTGCGGAGTCAAAATCTGCTGATATGATTTTCAAGTTGAAGAAGGAGATGGACGAGGAAGAAGTGGAGCCTAACGTGAATACATATCGAGAATTGATATCGTTATATTGCGGGATGGGGCATTGGAACAATGCATACAAGTTCTTCAGGGAAATGATTGAGGAGAAATGCTTAAAACCCAGCATGCCTCTTTATGAGATGGTTTTGCAACAACTAAGAAAGGCAGGACAGTTGAAGAAGCATGAGGAATTGGTTGATAAGATGGTTGAGAGAGGATTCGTGTCGCGGCCCCTGTAG

Protein sequence

MALIRSKPNLPLFLSYLSQRIQNHRSFSSSLSISESRLQDEPASDCPQNATNPVLSPEEIQAADKFHALIKEYYRRNPNPDPTPPCPNFTISALSNDLSQISALHSVSPAVVRYVIEKSGGVRHGIPFLQALAFFNWATAGEGFEHSAQPYNEMIDLAGKVKQFGLAWYLIDLMKARNVEITVETFSILVRRYVRAGLAAEAVHAFNRMEDYGCNPDKIAFSIVISILCKKRRAIEAQSFFDSLKQKFEPDVVVYTSLVHGWCRAGDISEAERVFKEMKMAGISPNVYTYSIVIDALCRSGQITRAHDVFSEMIDSGCNPNSVTFNNLIRVHVKAGRTEKVLQVYNQMKRLGCSPDIVTYNFLIETHCRDDNLGEAIKILNSMSKKGCTPNASTFNPIFRCIAKSQDVNGAHRMFAKMKDVGCKPNTVTYNILMRMFAESKSADMIFKLKKEMDEEEVEPNVNTYRELISLYCGMGHWNNAYKFFREMIEEKCLKPSMPLYEMVLQQLRKAGQLKKHEELVDKMVERGFVSRPL
Homology
BLAST of Lsi04G017760 vs. ExPASy Swiss-Prot
Match: Q9LN22 (Pentatricopeptide repeat-containing protein At1g20300, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At1g20300 PE=2 SV=1)

HSP 1 Score: 743.0 bits (1917), Expect = 2.3e-213
Identity = 365/539 (67.72%), Postives = 447/539 (82.93%), Query Frame = 0

Query: 1   MALIRSKPNLPLFLSYLSQRIQNHRSFSSSLSISESRLQDEPASDCPQNAT---NPVLSP 60
           MAL+RSK +L   LS++S  +    S S++  +S+    DE A+      +   +P+L+P
Sbjct: 1   MALLRSKLHLSRTLSFISPLLPKTFSTSATSLLSDHE-NDESAATITAAVSVPISPLLTP 60

Query: 61  EEIQAADKFHALIKEYYRRNP-NPDPTPPCPNFTISALSNDLSQISALHSVSPAVVRYVI 120
           E+ Q  +KFH++IK++YR+NP +P+     P+ T+ ALS D SQI     VSP+VVR VI
Sbjct: 61  EDTQTVEKFHSIIKDHYRKNPTSPNDAILNPSLTLHALSLDFSQIET-SQVSPSVVRCVI 120

Query: 121 EKSGGVRHGIPFLQALAFFNWATAGEGFEH-SAQPYNEMIDLAGKVKQFGLAWYLIDLMK 180
           EK G VRHGIP  Q+LAFFNWAT+ + ++H S  PYNEMIDL+GKV+QF LAW+LIDLMK
Sbjct: 121 EKCGSVRHGIPLHQSLAFFNWATSRDDYDHKSPHPYNEMIDLSGKVRQFDLAWHLIDLMK 180

Query: 181 ARNVEITVETFSILVRRYVRAGLAAEAVHAFNRMEDYGCNPDKIAFSIVISILCKKRRAI 240
           +RNVEI++ETF+IL+RRYVRAGLA+EAVH FNRMEDYGC PDKIAFSIVIS L +KRRA 
Sbjct: 181 SRNVEISIETFTILIRRYVRAGLASEAVHCFNRMEDYGCVPDKIAFSIVISNLSRKRRAS 240

Query: 241 EAQSFFDSLKQKFEPDVVVYTSLVHGWCRAGDISEAERVFKEMKMAGISPNVYTYSIVID 300
           EAQSFFDSLK +FEPDV+VYT+LV GWCRAG+ISEAE+VFKEMK+AGI PNVYTYSIVID
Sbjct: 241 EAQSFFDSLKDRFEPDVIVYTNLVRGWCRAGEISEAEKVFKEMKLAGIEPNVYTYSIVID 300

Query: 301 ALCRSGQITRAHDVFSEMIDSGCNPNSVTFNNLIRVHVKAGRTEKVLQVYNQMKRLGCSP 360
           ALCR GQI+RAHDVF++M+DSGC PN++TFNNL+RVHVKAGRTEKVLQVYNQMK+LGC P
Sbjct: 301 ALCRCGQISRAHDVFADMLDSGCAPNAITFNNLMRVHVKAGRTEKVLQVYNQMKKLGCEP 360

Query: 361 DIVTYNFLIETHCRDDNLGEAIKILNSMSKKGCTPNASTFNPIFRCIAKSQDVNGAHRMF 420
           D +TYNFLIE HCRD+NL  A+K+LN+M KK C  NASTFN IFR I K +DVNGAHRM+
Sbjct: 361 DTITYNFLIEAHCRDENLENAVKVLNTMIKKKCEVNASTFNTIFRYIEKKRDVNGAHRMY 420

Query: 421 AKMKDVGCKPNTVTYNILMRMFAESKSADMIFKLKKEMDEEEVEPNVNTYRELISLYCGM 480
           +KM +  C+PNTVTYNILMRMF  SKS DM+ K+KKEMD++EVEPNVNTYR L++++CGM
Sbjct: 421 SKMMEAKCEPNTVTYNILMRMFVGSKSTDMVLKMKKEMDDKEVEPNVNTYRLLVTMFCGM 480

Query: 481 GHWNNAYKFFREMIEEKCLKPSMPLYEMVLQQLRKAGQLKKHEELVDKMVERGFVSRPL 535
           GHWNNAYK F+EM+EEKCL PS+ LYEMVL QLR+AGQLKKHEELV+KM+++G V+RPL
Sbjct: 481 GHWNNAYKLFKEMVEEKCLTPSLSLYEMVLAQLRRAGQLKKHEELVEKMIQKGLVARPL 537

BLAST of Lsi04G017760 vs. ExPASy Swiss-Prot
Match: Q9C9A2 (Pentatricopeptide repeat-containing protein At1g71060, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At1g71060 PE=2 SV=1)

HSP 1 Score: 237.3 bits (604), Expect = 4.1e-61
Identity = 140/437 (32.04%), Postives = 232/437 (53.09%), Query Frame = 0

Query: 89  FTISALSNDLSQISALHSVSPAVVRYVIEKSGGVRHGIPFLQALAFFNWATAGEGFEHSA 148
           FT S +   L++ S    +SPA++  V++K          + AL+ F WA   +GF+H+ 
Sbjct: 76  FTDSKVETLLNEASV--KLSPALIEEVLKKLSNAG-----VLALSVFKWAENQKGFKHTT 135

Query: 149 QPYNEMIDLAGKVKQFGLAWYLIDLMKARNVEITVETFSILVRRYVRAGLAAEAVHAFNR 208
             YN +I+  GK+KQF L W L+D MKA+ + ++ ETF+++ RRY RA    EA+ AF++
Sbjct: 136 SNYNALIESLGKIKQFKLIWSLVDDMKAKKL-LSKETFALISRRYARARKVKEAIGAFHK 195

Query: 209 MEDYGCNPDKIAFSIVISILCKKRRAIEAQSFFDSLKQK-FEPDVVVYTSLVHGWCRAGD 268
           ME++G   +   F+ ++  L K R   +AQ  FD +K+K FEPD+  YT L+ GW +  +
Sbjct: 196 MEEFGFKMESSDFNRMLDTLSKSRNVGDAQKVFDKMKKKRFEPDIKSYTILLEGWGQELN 255

Query: 269 ISEAERVFKEMKMAGISPNVYTYSIVIDALCRSGQITRAHDVFSEMIDSGCNPNSVTFNN 328
           +   + V +EMK  G  P+V  Y I+I+A C++ +   A   F+EM    C P+   F +
Sbjct: 256 LLRVDEVNREMKDEGFEPDVVAYGIIINAHCKAKKYEEAIRFFNEMEQRNCKPSPHIFCS 315

Query: 329 LIRVHVKAGRTEKVLQVYNQMKRLGCSPDIVTYNFLIETHCRDDNLGEAIKILNSMSKKG 388
           LI       +    L+ + + K  G   +  TYN L+  +C    + +A K ++ M  KG
Sbjct: 316 LINGLGSEKKLNDALEFFERSKSSGFPLEAPTYNALVGAYCWSQRMEDAYKTVDEMRLKG 375

Query: 389 CTPNASTFNPIFRCIAKSQDVNGAHRMFAKMKDVGCKPNTVTYNILMRMFAESKSADMIF 448
             PNA T++ I   + + Q    A+ ++  M    C+P   TY I++RMF   +  DM  
Sbjct: 376 VGPNARTYDIILHHLIRMQRSKEAYEVYQTM---SCEPTVSTYEIMVRMFCNKERLDMAI 435

Query: 449 KLKKEMDEEEVEPNVNTYRELISLYCGMGHWNNAYKFFREMIEEKCLKPSMPLYEMVLQQ 508
           K+  EM  + V P ++ +  LI+  C     + A ++F EM++   ++P   ++  + Q 
Sbjct: 436 KIWDEMKGKGVLPGMHMFSSLITALCHENKLDEACEYFNEMLDVG-IRPPGHMFSRLKQT 495

Query: 509 LRKAGQLKKHEELVDKM 525
           L   G+  K  +LV KM
Sbjct: 496 LLDEGRKDKVTDLVVKM 500

BLAST of Lsi04G017760 vs. ExPASy Swiss-Prot
Match: Q9FVX2 (Pentatricopeptide repeat-containing protein At1g77360, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At1g77360 PE=2 SV=2)

HSP 1 Score: 235.7 bits (600), Expect = 1.2e-60
Identity = 124/377 (32.89%), Postives = 213/377 (56.50%), Query Frame = 0

Query: 134 FFNWATAGEGFEHSAQPYNEMIDLAGKVKQFGLAWYLIDLMKARNVEITVETFSILVRRY 193
           FF W+     +EHS + Y+ MI+   K++Q+ L W LI+ M+ + + + VETF I++R+Y
Sbjct: 120 FFQWSEKQRHYEHSVRAYHMMIESTAKIRQYKLMWDLINAMRKKKM-LNVETFCIVMRKY 179

Query: 194 VRAGLAAEAVHAFNRMEDYGCNPDKIAFSIVISILCKKRRAIEAQSFFDSLKQKFEPDVV 253
            RA    EA++AFN ME Y   P+ +AF+ ++S LCK +   +AQ  F++++ +F PD  
Sbjct: 180 ARAQKVDEAIYAFNVMEKYDLPPNLVAFNGLLSALCKSKNVRKAQEVFENMRDRFTPDSK 239

Query: 254 VYTSLVHGWCRAGDISEAERVFKEMKMAGISPNVYTYSIVIDALCRSGQITRAHDVFSEM 313
            Y+ L+ GW +  ++ +A  VF+EM  AG  P++ TYSI++D LC++G++  A  +   M
Sbjct: 240 TYSILLEGWGKEPNLPKAREVFREMIDAGCHPDIVTYSIMVDILCKAGRVDEALGIVRSM 299

Query: 314 IDSGCNPNSVTFNNLIRVHVKAGRTEKVLQVYNQMKRLGCSPDIVTYNFLIETHCRDDNL 373
             S C P +  ++ L+  +    R E+ +  + +M+R G   D+  +N LI   C+ + +
Sbjct: 300 DPSICKPTTFIYSVLVHTYGTENRLEEAVDTFLEMERSGMKADVAVFNSLIGAFCKANRM 359

Query: 374 GEAIKILNSMSKKGCTPNASTFNPIFRCIAKSQDVNGAHRMFAKMKDVGCKPNTVTYNIL 433
               ++L  M  KG TPN+ + N I R + +  + + A  +F KM  V C+P+  TY ++
Sbjct: 360 KNVYRVLKEMKSKGVTPNSKSCNIILRHLIERGEKDEAFDVFRKMIKV-CEPDADTYTMV 419

Query: 434 MRMFAESKSADMIFKLKKEMDEEEVEPNVNTYRELISLYCGMGHWNNAYKFFREMIEEKC 493
           ++MF E K  +   K+ K M ++ V P+++T+  LI+  C       A     EMI E  
Sbjct: 420 IKMFCEKKEMETADKVWKYMRKKGVFPSMHTFSVLINGLCEERTTQKACVLLEEMI-EMG 479

Query: 494 LKPSMPLYEMVLQQLRK 511
           ++PS   +  + Q L K
Sbjct: 480 IRPSGVTFGRLRQLLIK 493

BLAST of Lsi04G017760 vs. ExPASy Swiss-Prot
Match: P0C8A0 (Pentatricopeptide repeat-containing protein At3g49730 OS=Arabidopsis thaliana OX=3702 GN=At3g49730 PE=2 SV=1)

HSP 1 Score: 221.5 bits (563), Expect = 2.3e-56
Identity = 112/358 (31.28%), Postives = 191/358 (53.35%), Query Frame = 0

Query: 134 FFNWATAGEGFEHSAQPYNEMIDLAGKVKQFGLAWYLIDLMKARNVE-ITVETFSILVRR 193
           FF WAT   G+ HS +    M+ +  K++QFG  W LI+ M+  N E I  E F +L+RR
Sbjct: 117 FFLWATKQPGYFHSYEVCKSMVMILSKMRQFGAVWGLIEEMRKTNPELIEPELFVVLMRR 176

Query: 194 YVRAGLAAEAVHAFNRMEDYGCNPDKIAFSIVISILCKKRRAIEAQSFFDSLKQKFEPDV 253
           +  A +  +AV   + M  YG  PD+  F  ++  LCK     EA   F+ +++KF P++
Sbjct: 177 FASANMVKKAVEVLDEMPKYGLEPDEYVFGCLLDALCKNGSVKEASKVFEDMREKFPPNL 236

Query: 254 VVYTSLVHGWCRAGDISEAERVFKEMKMAGISPNVYTYSIVIDALCRSGQITRAHDVFSE 313
             +TSL++GWCR G + EA+ V  +MK AG+ P++  ++ ++     +G++  A+D+ ++
Sbjct: 237 RYFTSLLYGWCREGKLMEAKEVLVQMKEAGLEPDIVVFTNLLSGYAHAGKMADAYDLMND 296

Query: 314 MIDSGCNPNSVTFNNLIRVHVKA-GRTEKVLQVYNQMKRLGCSPDIVTYNFLIETHCRDD 373
           M   G  PN   +  LI+   +   R ++ ++V+ +M+R GC  DIVTY  LI   C+  
Sbjct: 297 MRKRGFEPNVNCYTVLIQALCRTEKRMDEAMRVFVEMERYGCEADIVTYTALISGFCKWG 356

Query: 374 NLGEAIKILNSMSKKGCTPNASTFNPIFRCIAKSQDVNGAHRMFAKMKDVGCKPNTVTYN 433
            + +   +L+ M KKG  P+  T+  I     K +       +  KMK  GC P+ + YN
Sbjct: 357 MIDKGYSVLDDMRKKGVMPSQVTYMQIMVAHEKKEQFEECLELIEKMKRRGCHPDLLIYN 416

Query: 434 ILMRMFAESKSADMIFKLKKEMDEEEVEPNVNTYRELISLYCGMGHWNNAYKFFREMI 490
           +++R+  +        +L  EM+   + P V+T+  +I+ +   G    A   F+EM+
Sbjct: 417 VVIRLACKLGEVKEAVRLWNEMEANGLSPGVDTFVIMINGFTSQGFLIEACNHFKEMV 474

BLAST of Lsi04G017760 vs. ExPASy Swiss-Prot
Match: Q9S7R4 (Pentatricopeptide repeat-containing protein At1g74900, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=OTP43 PE=2 SV=1)

HSP 1 Score: 211.1 bits (536), Expect = 3.1e-53
Identity = 105/342 (30.70%), Postives = 186/342 (54.39%), Query Frame = 0

Query: 130 QALAFFNWA-TAGEGFEHSAQPYNEMIDLAGKVKQFGLAWYLIDLMKARNVEITVETFSI 189
           +AL FF++       + H A  ++  ID+A ++      W LI  M++  +  + +TF+I
Sbjct: 72  KALQFFHFLDNHHREYVHDASSFDLAIDIAARLHLHPTVWSLIHRMRSLRIGPSPKTFAI 131

Query: 190 LVRRYVRAGLAAEAVHAFNRMEDYGCNPDKIAFSIVISILCKKRRAIEAQSFFDSLKQKF 249
           +  RY  AG   +AV  F  M ++GC  D  +F+ ++ +LCK +R  +A   F +L+ +F
Sbjct: 132 VAERYASAGKPDKAVKLFLNMHEHGCFQDLASFNTILDVLCKSKRVEKAYELFRALRGRF 191

Query: 250 EPDVVVYTSLVHGWCRAGDISEAERVFKEMKMAGISPNVYTYSIVIDALCRSGQITRAHD 309
             D V Y  +++GWC      +A  V KEM   GI+PN+ TY+ ++    R+GQI  A +
Sbjct: 192 SVDTVTYNVILNGWCLIKRTPKALEVLKEMVERGINPNLTTYNTMLKGFFRAGQIRHAWE 251

Query: 310 VFSEMIDSGCNPNSVTFNNLIRVHVKAGRTEKVLQVYNQMKRLGCSPDIVTYNFLIETHC 369
            F EM    C  + VT+  ++     AG  ++   V+++M R G  P + TYN +I+  C
Sbjct: 252 FFLEMKKRDCEIDVVTYTTVVHGFGVAGEIKRARNVFDEMIREGVLPSVATYNAMIQVLC 311

Query: 370 RDDNLGEAIKILNSMSKKGCTPNASTFNPIFRCIAKSQDVNGAHRMFAKMKDVGCKPNTV 429
           + DN+  A+ +   M ++G  PN +T+N + R +  + + +    +  +M++ GC+PN  
Sbjct: 312 KKDNVENAVVMFEEMVRRGYEPNVTTYNVLIRGLFHAGEFSRGEELMQRMENEGCEPNFQ 371

Query: 430 TYNILMRMFAESKSADMIFKLKKEMDEEEVEPNVNTYRELIS 471
           TYN+++R ++E    +    L ++M   +  PN++TY  LIS
Sbjct: 372 TYNMMIRYYSECSEVEKALGLFEKMGSGDCLPNLDTYNILIS 413

BLAST of Lsi04G017760 vs. ExPASy TrEMBL
Match: A0A6J1CG38 (pentatricopeptide repeat-containing protein At1g20300, mitochondrial isoform X1 OS=Momordica charantia OX=3673 GN=LOC111011204 PE=3 SV=1)

HSP 1 Score: 983.0 bits (2540), Expect = 4.9e-283
Identity = 485/540 (89.81%), Postives = 510/540 (94.44%), Query Frame = 0

Query: 1   MALIRSKPNLPLFLSYLSQRIQNHRSFSSSLSISES------RLQDEPASDCPQNATNPV 60
           MALIRSKPNLP FLS LS RIQNHRSFSSS SISE+      + QDE AS  PQNA+ P+
Sbjct: 1   MALIRSKPNLPRFLSSLSHRIQNHRSFSSSPSISEADAEVHLQNQDEDASQSPQNASKPL 60

Query: 61  LSPEEIQAADKFHALIKEYYRRNPNPDPTPPCPNFTISALSNDLSQISALHSVSPAVVRY 120
           LSPEE+Q ADKF+ALIKEYYRRNPNPDPTPPCPNFTISA SN+LS ISA+HSVSPAVVR+
Sbjct: 61  LSPEEMQTADKFYALIKEYYRRNPNPDPTPPCPNFTISAFSNELSHISAVHSVSPAVVRH 120

Query: 121 VIEKSGGVRHGIPFLQALAFFNWATAGEGFEHSAQPYNEMIDLAGKVKQFGLAWYLIDLM 180
           VIEKSGGVRHGIPFLQALAFFNWATA EGF+HSA+PYNEMIDLAGKV+QFGLAW+LIDLM
Sbjct: 121 VIEKSGGVRHGIPFLQALAFFNWATAREGFQHSAEPYNEMIDLAGKVRQFGLAWHLIDLM 180

Query: 181 KARNVEITVETFSILVRRYVRAGLAAEAVHAFNRMEDYGCNPDKIAFSIVISILCKKRRA 240
           KAR VEITVETFSILVRRYVRAGLAAEAVHAFNRMEDYGCNPDKIAFSIV+SILCKKRRA
Sbjct: 181 KARTVEITVETFSILVRRYVRAGLAAEAVHAFNRMEDYGCNPDKIAFSIVVSILCKKRRA 240

Query: 241 IEAQSFFDSLKQKFEPDVVVYTSLVHGWCRAGDISEAERVFKEMKMAGISPNVYTYSIVI 300
            EAQSFFDSLK KFEPDVVVYTSLVHGWCRAG+ISEAERVFKEMKMAGISPNVYTYSIVI
Sbjct: 241 SEAQSFFDSLKHKFEPDVVVYTSLVHGWCRAGEISEAERVFKEMKMAGISPNVYTYSIVI 300

Query: 301 DALCRSGQITRAHDVFSEMIDSGCNPNSVTFNNLIRVHVKAGRTEKVLQVYNQMKRLGCS 360
           DALCR GQITRAHDVFSEMIDSGCNPNSVTFNNLIRVHVKAGRTEKVLQVYNQM+RLGC+
Sbjct: 301 DALCRCGQITRAHDVFSEMIDSGCNPNSVTFNNLIRVHVKAGRTEKVLQVYNQMRRLGCA 360

Query: 361 PDIVTYNFLIETHCRDDNLGEAIKILNSMSKKGCTPNASTFNPIFRCIAKSQDVNGAHRM 420
            DI+TYNF+IETHCRDDNLGEAIK+LNSM+KKGC+PNASTFNPIFRCIAKSQDVN AHRM
Sbjct: 361 ADIITYNFIIETHCRDDNLGEAIKVLNSMAKKGCSPNASTFNPIFRCIAKSQDVNSAHRM 420

Query: 421 FAKMKDVGCKPNTVTYNILMRMFAESKSADMIFKLKKEMDEEEVEPNVNTYRELISLYCG 480
           FAKMKD+GCK NTVTYNILMRMFAESKSADMIFKLKKEMDEEEVEPNVNTYR LISLYCG
Sbjct: 421 FAKMKDLGCKANTVTYNILMRMFAESKSADMIFKLKKEMDEEEVEPNVNTYRVLISLYCG 480

Query: 481 MGHWNNAYKFFREMIEEKCLKPSMPLYEMVLQQLRKAGQLKKHEELVDKMVERGFVSRPL 535
           MGHWNNAYKFFREMIEEKCLKPSMP+Y+MVLQ LR AGQLKKHEELVDKMVERGF SRPL
Sbjct: 481 MGHWNNAYKFFREMIEEKCLKPSMPIYQMVLQLLRNAGQLKKHEELVDKMVERGFASRPL 540

BLAST of Lsi04G017760 vs. ExPASy TrEMBL
Match: A0A6J1FLC3 (pentatricopeptide repeat-containing protein At1g20300, mitochondrial OS=Cucurbita moschata OX=3662 GN=LOC111446459 PE=4 SV=1)

HSP 1 Score: 978.0 bits (2527), Expect = 1.6e-281
Identity = 479/534 (89.70%), Postives = 505/534 (94.57%), Query Frame = 0

Query: 1   MALIRSKPNLPLFLSYLSQRIQNHRSFSSSLSISESRLQDEPASDCPQNATNPVLSPEEI 60
           MAL +SKPNLP FLS LS  +QNHR FSSS SISES+LQDE AS+    A+ PVL PEEI
Sbjct: 1   MALFKSKPNLPPFLSSLSPFLQNHRLFSSSTSISESQLQDEDASNTTPKASKPVLLPEEI 60

Query: 61  QAADKFHALIKEYYRRNPNPDPTPPCPNFTISALSNDLSQISALHSVSPAVVRYVIEKSG 120
           QAADKFH+LIKEYYRRNP+PDPTPP PNFTISALSN+LSQIS +H+VSP VVRYVIEKSG
Sbjct: 61  QAADKFHSLIKEYYRRNPSPDPTPPSPNFTISALSNELSQISPVHAVSPGVVRYVIEKSG 120

Query: 121 GVRHGIPFLQALAFFNWATAGEGFEHSAQPYNEMIDLAGKVKQFGLAWYLIDLMKARNVE 180
           GVRHGIPFLQALAFFNWATA E FEHSAQPYNEMIDLAGKVKQFGLAW+LIDLMKARNVE
Sbjct: 121 GVRHGIPFLQALAFFNWATASETFEHSAQPYNEMIDLAGKVKQFGLAWHLIDLMKARNVE 180

Query: 181 ITVETFSILVRRYVRAGLAAEAVHAFNRMEDYGCNPDKIAFSIVISILCKKRRAIEAQSF 240
           ITVETFSILVRRYVRAGLAAEAVHAFNRMEDYGCNPDKIAF+IVISILCKKRRA EAQSF
Sbjct: 181 ITVETFSILVRRYVRAGLAAEAVHAFNRMEDYGCNPDKIAFTIVISILCKKRRATEAQSF 240

Query: 241 FDSLKQKFEPDVVVYTSLVHGWCRAGDISEAERVFKEMKMAGISPNVYTYSIVIDALCRS 300
           FD+LK KFEPDV+VYT+LVHGWCRAGDISEAERVFKEMKMAGISPNVYTYSIVIDALCR 
Sbjct: 241 FDNLKHKFEPDVIVYTNLVHGWCRAGDISEAERVFKEMKMAGISPNVYTYSIVIDALCRC 300

Query: 301 GQITRAHDVFSEMIDSGCNPNSVTFNNLIRVHVKAGRTEKVLQVYNQMKRLGCSPDIVTY 360
           GQITRAHDVFSEMID+GCNPNSVTFNNLIRVHVKAGRTEKVLQVYNQMKRLGC+ DI+TY
Sbjct: 301 GQITRAHDVFSEMIDAGCNPNSVTFNNLIRVHVKAGRTEKVLQVYNQMKRLGCAADIITY 360

Query: 361 NFLIETHCRDDNLGEAIKILNSMSKKGCTPNASTFNPIFRCIAKSQDVNGAHRMFAKMKD 420
           NFLIETHC+DDNLGEA K+LNSM+KKGCTPNASTFNPIFRCI KS DVNG HRMFAKMKD
Sbjct: 361 NFLIETHCKDDNLGEATKVLNSMAKKGCTPNASTFNPIFRCIVKSHDVNGVHRMFAKMKD 420

Query: 421 VGCKPNTVTYNILMRMFAESKSADMIFKLKKEMDEEEVEPNVNTYRELISLYCGMGHWNN 480
           +GCKPNTVTYNILMRMFAESKSADMIFKLKKEM+EEEVEPN+NTY+ LISLYCGMGHWNN
Sbjct: 421 LGCKPNTVTYNILMRMFAESKSADMIFKLKKEMEEEEVEPNLNTYQVLISLYCGMGHWNN 480

Query: 481 AYKFFREMIEEKCLKPSMPLYEMVLQQLRKAGQLKKHEELVDKMVERGFVSRPL 535
           AYKFFREMIEEKCLKPSMP+YEMVLQQLRKAGQLKKHEELVDKMVERGF SRPL
Sbjct: 481 AYKFFREMIEEKCLKPSMPIYEMVLQQLRKAGQLKKHEELVDKMVERGFASRPL 534

BLAST of Lsi04G017760 vs. ExPASy TrEMBL
Match: A0A6J1J1A0 (pentatricopeptide repeat-containing protein At1g20300, mitochondrial OS=Cucurbita maxima OX=3661 GN=LOC111480961 PE=4 SV=1)

HSP 1 Score: 968.4 bits (2502), Expect = 1.2e-278
Identity = 475/534 (88.95%), Postives = 504/534 (94.38%), Query Frame = 0

Query: 1   MALIRSKPNLPLFLSYLSQRIQNHRSFSSSLSISESRLQDEPASDCPQNATNPVLSPEEI 60
           MAL +SKPNL  FLS LS  +QNHR FSSS SISES+LQDE AS+   NA+ PVL PEEI
Sbjct: 1   MALFKSKPNLSPFLSSLSPFLQNHRLFSSSTSISESQLQDEDASNTTPNASKPVLLPEEI 60

Query: 61  QAADKFHALIKEYYRRNPNPDPTPPCPNFTISALSNDLSQISALHSVSPAVVRYVIEKSG 120
           QAA KFH+LIKEYYRRNP+PDPTPP PNFTISALSN+LSQIS +H+VSPAVVRYVIEKSG
Sbjct: 61  QAAGKFHSLIKEYYRRNPSPDPTPPSPNFTISALSNELSQISPVHAVSPAVVRYVIEKSG 120

Query: 121 GVRHGIPFLQALAFFNWATAGEGFEHSAQPYNEMIDLAGKVKQFGLAWYLIDLMKARNVE 180
           GVRHGIPFLQALAFFNWATA E F+HSA PYN+MIDLAGKV+QFGLAW+LIDLMKARNVE
Sbjct: 121 GVRHGIPFLQALAFFNWATASETFDHSALPYNQMIDLAGKVRQFGLAWHLIDLMKARNVE 180

Query: 181 ITVETFSILVRRYVRAGLAAEAVHAFNRMEDYGCNPDKIAFSIVISILCKKRRAIEAQSF 240
           ITVETFSILVRRYVRAGLAAEAVHAFNRMEDYGCNPDKIAFSIVISIL KKRRA EAQSF
Sbjct: 181 ITVETFSILVRRYVRAGLAAEAVHAFNRMEDYGCNPDKIAFSIVISILSKKRRATEAQSF 240

Query: 241 FDSLKQKFEPDVVVYTSLVHGWCRAGDISEAERVFKEMKMAGISPNVYTYSIVIDALCRS 300
           FD+LK KFEPDV+VYTSLVHGWCRAGDISEAERVFKEMKMAGISPNVYTYSIVIDALCR 
Sbjct: 241 FDNLKHKFEPDVIVYTSLVHGWCRAGDISEAERVFKEMKMAGISPNVYTYSIVIDALCRC 300

Query: 301 GQITRAHDVFSEMIDSGCNPNSVTFNNLIRVHVKAGRTEKVLQVYNQMKRLGCSPDIVTY 360
           GQITRAHDVFSEM+D+GCNPNSVTFNNLIRVHVKAGRTEKVLQVYNQMKRLGC+ DI+TY
Sbjct: 301 GQITRAHDVFSEMVDAGCNPNSVTFNNLIRVHVKAGRTEKVLQVYNQMKRLGCAADIITY 360

Query: 361 NFLIETHCRDDNLGEAIKILNSMSKKGCTPNASTFNPIFRCIAKSQDVNGAHRMFAKMKD 420
           NFLIETHC+DDNLGEA K+LNSM+KKGCTPNASTFNPIFRCIAKS DVNG HRMFA+MKD
Sbjct: 361 NFLIETHCKDDNLGEATKVLNSMAKKGCTPNASTFNPIFRCIAKSHDVNGVHRMFARMKD 420

Query: 421 VGCKPNTVTYNILMRMFAESKSADMIFKLKKEMDEEEVEPNVNTYRELISLYCGMGHWNN 480
           +GCKPNTVTYNILMRMFAESKSADMIFKLKKEM+EEEVEPN+NTY+ LISLYCGMGHWNN
Sbjct: 421 LGCKPNTVTYNILMRMFAESKSADMIFKLKKEMEEEEVEPNLNTYQVLISLYCGMGHWNN 480

Query: 481 AYKFFREMIEEKCLKPSMPLYEMVLQQLRKAGQLKKHEELVDKMVERGFVSRPL 535
           AYKFFREMIEEKCLKPSMP+YEMVLQQLRKAGQLKKHEELVDKMVERGF SRPL
Sbjct: 481 AYKFFREMIEEKCLKPSMPIYEMVLQQLRKAGQLKKHEELVDKMVERGFASRPL 534

BLAST of Lsi04G017760 vs. ExPASy TrEMBL
Match: A0A5A7V5E7 (Calcium-dependent protein kinase 29 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold205G001380 PE=3 SV=1)

HSP 1 Score: 952.2 bits (2460), Expect = 9.2e-274
Identity = 468/534 (87.64%), Postives = 501/534 (93.82%), Query Frame = 0

Query: 1    MALIRSKPNLPLFLSYLSQRIQNHRSFSSSLSISESRLQDEPASDCPQNATNPVLSPEEI 60
            MALI+SK NLP FLS LS RIQNHRSFS S SIS+S LQDE ASD PQN +NPVLSP++I
Sbjct: 528  MALIKSKLNLPPFLSSLSHRIQNHRSFSFSPSISDSPLQDELASDSPQNPSNPVLSPDQI 587

Query: 61   QAADKFHALIKEYYRRNPNPDPTPPCPNFTISALSNDLSQISALHSVSPAVVRYVIEKSG 120
            Q ++KFHALIKEYYRRNP+PD TPP PNFTIS+LSNDLSQISA HSVSPAVVRYVIEKSG
Sbjct: 588  QVSEKFHALIKEYYRRNPSPDSTPPSPNFTISSLSNDLSQISAPHSVSPAVVRYVIEKSG 647

Query: 121  GVRHGIPFLQALAFFNWATAGEGFEHSAQPYNEMIDLAGKVKQFGLAWYLIDLMKARNVE 180
             VRHGIPFL ALAFFNW TAGEGFEHS QPYNEMIDLAGKV+QFGLAWYLIDLMKARNVE
Sbjct: 648  AVRHGIPFLPALAFFNWVTAGEGFEHSTQPYNEMIDLAGKVRQFGLAWYLIDLMKARNVE 707

Query: 181  ITVETFSILVRRYVRAGLAAEAVHAFNRMEDYGCNPDKIAFSIVISILCKKRRAIEAQSF 240
            ITVETFSILVRRYVRAGLAAEAVHAFNRME+YGC  D +AFSIVISILCKKRRA+EAQSF
Sbjct: 708  ITVETFSILVRRYVRAGLAAEAVHAFNRMEEYGCTADTVAFSIVISILCKKRRAVEAQSF 767

Query: 241  FDSLKQKFEPDVVVYTSLVHGWCRAGDISEAERVFKEMKMAGISPNVYTYSIVIDALCRS 300
            FD+LK KFEPDVVVYTSLVHGWCRAGDISEAERVFKEMKMAGISPNVYTYSIVIDALCRS
Sbjct: 768  FDNLKHKFEPDVVVYTSLVHGWCRAGDISEAERVFKEMKMAGISPNVYTYSIVIDALCRS 827

Query: 301  GQITRAHDVFSEMIDSGCNPNSVTFNNLIRVHVKAGRTEKVLQVYNQMKRLGCSPDIVTY 360
            GQITRAHDVF+EM+++GCNPNSVTFNNLIRVHV+AGRTEKVLQVYNQM+RL C+ D++TY
Sbjct: 828  GQITRAHDVFAEMLNAGCNPNSVTFNNLIRVHVRAGRTEKVLQVYNQMRRLRCAADLITY 887

Query: 361  NFLIETHCRDDNLGEAIKILNSMSKKGCTPNASTFNPIFRCIAKSQDVNGAHRMFAKMKD 420
            NFLIETHC+D+NLGEAIK+LNSM K GCTP+AS+FNPIFRCIAKSQDVNGAHRMFA+MKD
Sbjct: 888  NFLIETHCKDENLGEAIKVLNSMIKNGCTPDASSFNPIFRCIAKSQDVNGAHRMFARMKD 947

Query: 421  VGCKPNTVTYNILMRMFAESKSADMIFKLKKEMDEEEVEPNVNTYRELISLYCGMGHWNN 480
            VGCKPNT TYNILMRMFA  KSADMIFKLKKEMDEEEVEPNVNTYRELI+LYCGMGHWNN
Sbjct: 948  VGCKPNTATYNILMRMFAVPKSADMIFKLKKEMDEEEVEPNVNTYRELITLYCGMGHWNN 1007

Query: 481  AYKFFREMIEEKCLKPSMPLYEMVLQQLRKAGQLKKHEELVDKMVERGFVSRPL 535
            AYKFFREMIEEK LKPSM LY+MVL+QLR+AGQLKKHEELVDKMVERGF SR L
Sbjct: 1008 AYKFFREMIEEKNLKPSMSLYKMVLEQLREAGQLKKHEELVDKMVERGFASRNL 1061

BLAST of Lsi04G017760 vs. ExPASy TrEMBL
Match: A0A0A0LE95 (PPR_long domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G874400 PE=3 SV=1)

HSP 1 Score: 951.4 bits (2458), Expect = 1.6e-273
Identity = 465/534 (87.08%), Postives = 501/534 (93.82%), Query Frame = 0

Query: 1   MALIRSKPNLPLFLSYLSQRIQNHRSFSSSLSISESRLQDEPASDCPQNATNPVLSPEEI 60
           MALI+SK NLP FLS LS RIQNHRSFSSS SIS+  LQD+ A+D PQNA+ P+LSPE+I
Sbjct: 1   MALIKSKLNLPPFLSSLSHRIQNHRSFSSSPSISDPPLQDDLATDSPQNASIPLLSPEQI 60

Query: 61  QAADKFHALIKEYYRRNPNPDPTPPCPNFTISALSNDLSQISALHSVSPAVVRYVIEKSG 120
           Q ++KFHALIKEYYRRNP PD TPPCPNFTIS+LSNDLSQISA HSVSPAVVRYVIEKSG
Sbjct: 61  QVSEKFHALIKEYYRRNPGPDSTPPCPNFTISSLSNDLSQISAPHSVSPAVVRYVIEKSG 120

Query: 121 GVRHGIPFLQALAFFNWATAGEGFEHSAQPYNEMIDLAGKVKQFGLAWYLIDLMKARNVE 180
            VRHGIPFL ALAFFNWATAGEGFEHS QPYNEMIDLAGKVKQFGLAWYLIDLMKARNVE
Sbjct: 121 AVRHGIPFLPALAFFNWATAGEGFEHSPQPYNEMIDLAGKVKQFGLAWYLIDLMKARNVE 180

Query: 181 ITVETFSILVRRYVRAGLAAEAVHAFNRMEDYGCNPDKIAFSIVISILCKKRRAIEAQSF 240
           ITV TFS+LVRRYVRAGLAAEAVHAFNRMEDYGCN D IAFS VISILCKKRRA+EAQSF
Sbjct: 181 ITVVTFSMLVRRYVRAGLAAEAVHAFNRMEDYGCNADIIAFSNVISILCKKRRAVEAQSF 240

Query: 241 FDSLKQKFEPDVVVYTSLVHGWCRAGDISEAERVFKEMKMAGISPNVYTYSIVIDALCRS 300
           FD+LK KFEPDV+VYTSLVHGWCRAGDISEAE VF+EMKMAGISPNVYTYSIVIDALCRS
Sbjct: 241 FDNLKHKFEPDVIVYTSLVHGWCRAGDISEAESVFREMKMAGISPNVYTYSIVIDALCRS 300

Query: 301 GQITRAHDVFSEMIDSGCNPNSVTFNNLIRVHVKAGRTEKVLQVYNQMKRLGCSPDIVTY 360
           GQITRAHDVF+EM+D+GCNPNSVTFNNLIRVH++AGRTEKVLQVYNQMKRL C+ D++TY
Sbjct: 301 GQITRAHDVFAEMLDAGCNPNSVTFNNLIRVHLRAGRTEKVLQVYNQMKRLRCAADLITY 360

Query: 361 NFLIETHCRDDNLGEAIKILNSMSKKGCTPNASTFNPIFRCIAKSQDVNGAHRMFAKMKD 420
           NFLIETHC+DDNLGEAIK+LNSM+K  CTPNAS+FNPIFRCIAKSQDVNGAHRMFA+MK+
Sbjct: 361 NFLIETHCKDDNLGEAIKVLNSMAKNDCTPNASSFNPIFRCIAKSQDVNGAHRMFARMKE 420

Query: 421 VGCKPNTVTYNILMRMFAESKSADMIFKLKKEMDEEEVEPNVNTYRELISLYCGMGHWNN 480
           VGCKPNTVTYNILMRMFA  KSADMIFKLKKEMDEEEVEPN NTYRELI+LYCGMGHWN+
Sbjct: 421 VGCKPNTVTYNILMRMFAVPKSADMIFKLKKEMDEEEVEPNFNTYRELIALYCGMGHWNH 480

Query: 481 AYKFFREMIEEKCLKPSMPLYEMVLQQLRKAGQLKKHEELVDKMVERGFVSRPL 535
           AY FFREMI+EKC+KPSMPLY+MVL++LRKAGQLKKHEELVDKMVERGF SR L
Sbjct: 481 AYMFFREMIDEKCIKPSMPLYKMVLEELRKAGQLKKHEELVDKMVERGFASRNL 534

BLAST of Lsi04G017760 vs. NCBI nr
Match: XP_038877723.1 (pentatricopeptide repeat-containing protein At1g20300, mitochondrial [Benincasa hispida])

HSP 1 Score: 1002.7 bits (2591), Expect = 1.2e-288
Identity = 494/532 (92.86%), Postives = 512/532 (96.24%), Query Frame = 0

Query: 1   MALIRSKPNLPLFLSYLSQRIQNHRSFSSSLSISESRLQDEPASDCPQNATNPVLSPEEI 60
           MALI+SKPNLP FLS LSQRIQNHRSFSS  SISES LQDEPASD PQNA+ PVLSPE+I
Sbjct: 1   MALIKSKPNLPSFLSSLSQRIQNHRSFSSCPSISESSLQDEPASDSPQNASKPVLSPEQI 60

Query: 61  QAADKFHALIKEYYRRNPNPDPTPPCPNFTISALSNDLSQISALHSVSPAVVRYVIEKSG 120
           QAA+K+HALIKEYYRRNPNPD TPPCPNFTIS+LSNDLSQI+ALHS+SPAVVRYV+EKSG
Sbjct: 61  QAANKYHALIKEYYRRNPNPDHTPPCPNFTISSLSNDLSQIAALHSISPAVVRYVVEKSG 120

Query: 121 GVRHGIPFLQALAFFNWATAGEGFEHSAQPYNEMIDLAGKVKQFGLAWYLIDLMKARNVE 180
            VRHGIPFLQALAFFNWATA EGFEHS  PY EMIDLAGKV+QFGLAWYLIDLMKARNVE
Sbjct: 121 AVRHGIPFLQALAFFNWATAVEGFEHSTHPYIEMIDLAGKVRQFGLAWYLIDLMKARNVE 180

Query: 181 ITVETFSILVRRYVRAGLAAEAVHAFNRMEDYGCNPDKIAFSIVISILCKKRRAIEAQSF 240
           ITVETFSILVRRYVRAGL AEAVHAFNRMEDYGCNPDKIAFSIVISILCKKRRA EAQSF
Sbjct: 181 ITVETFSILVRRYVRAGLTAEAVHAFNRMEDYGCNPDKIAFSIVISILCKKRRATEAQSF 240

Query: 241 FDSLKQKFEPDVVVYTSLVHGWCRAGDISEAERVFKEMKMAGISPNVYTYSIVIDALCRS 300
           FDSLK KFEPDVVVYTSLVHGWCRAGDIS AERVFKEMKMAGISPNVYTYSIVIDALCRS
Sbjct: 241 FDSLKHKFEPDVVVYTSLVHGWCRAGDISGAERVFKEMKMAGISPNVYTYSIVIDALCRS 300

Query: 301 GQITRAHDVFSEMIDSGCNPNSVTFNNLIRVHVKAGRTEKVLQVYNQMKRLGCSPDIVTY 360
           GQITRAHDVFSEMID+GCNPNSVTFNNLIRVHVKAGRTEKVLQVYNQMKRLGC+ DIVTY
Sbjct: 301 GQITRAHDVFSEMIDAGCNPNSVTFNNLIRVHVKAGRTEKVLQVYNQMKRLGCAADIVTY 360

Query: 361 NFLIETHCRDDNLGEAIKILNSMSKKGCTPNASTFNPIFRCIAKSQDVNGAHRMFAKMKD 420
           NFLIETHCRDDNLGEAIK+LNSM+KKGCTPNASTFNPIFRCIAKS+DVNGAHRMFAKMK+
Sbjct: 361 NFLIETHCRDDNLGEAIKVLNSMAKKGCTPNASTFNPIFRCIAKSRDVNGAHRMFAKMKE 420

Query: 421 VGCKPNTVTYNILMRMFAESKSADMIFKLKKEMDEEEVEPNVNTYRELISLYCGMGHWNN 480
           VGCKPNTVTYNILMRMFAESKSADMIFKLKKEMDEEEVEPNVNTYRELISLYCGMGHWNN
Sbjct: 421 VGCKPNTVTYNILMRMFAESKSADMIFKLKKEMDEEEVEPNVNTYRELISLYCGMGHWNN 480

Query: 481 AYKFFREMIEEKCLKPSMPLYEMVLQQLRKAGQLKKHEELVDKMVERGFVSR 533
           AYKFFREMIEEKCLKPSM LY+MVL+QLRKAGQLKKHEELVDKMVERGF SR
Sbjct: 481 AYKFFREMIEEKCLKPSMSLYKMVLEQLRKAGQLKKHEELVDKMVERGFTSR 532

BLAST of Lsi04G017760 vs. NCBI nr
Match: XP_022140589.1 (pentatricopeptide repeat-containing protein At1g20300, mitochondrial isoform X1 [Momordica charantia])

HSP 1 Score: 983.0 bits (2540), Expect = 1.0e-282
Identity = 485/540 (89.81%), Postives = 510/540 (94.44%), Query Frame = 0

Query: 1   MALIRSKPNLPLFLSYLSQRIQNHRSFSSSLSISES------RLQDEPASDCPQNATNPV 60
           MALIRSKPNLP FLS LS RIQNHRSFSSS SISE+      + QDE AS  PQNA+ P+
Sbjct: 1   MALIRSKPNLPRFLSSLSHRIQNHRSFSSSPSISEADAEVHLQNQDEDASQSPQNASKPL 60

Query: 61  LSPEEIQAADKFHALIKEYYRRNPNPDPTPPCPNFTISALSNDLSQISALHSVSPAVVRY 120
           LSPEE+Q ADKF+ALIKEYYRRNPNPDPTPPCPNFTISA SN+LS ISA+HSVSPAVVR+
Sbjct: 61  LSPEEMQTADKFYALIKEYYRRNPNPDPTPPCPNFTISAFSNELSHISAVHSVSPAVVRH 120

Query: 121 VIEKSGGVRHGIPFLQALAFFNWATAGEGFEHSAQPYNEMIDLAGKVKQFGLAWYLIDLM 180
           VIEKSGGVRHGIPFLQALAFFNWATA EGF+HSA+PYNEMIDLAGKV+QFGLAW+LIDLM
Sbjct: 121 VIEKSGGVRHGIPFLQALAFFNWATAREGFQHSAEPYNEMIDLAGKVRQFGLAWHLIDLM 180

Query: 181 KARNVEITVETFSILVRRYVRAGLAAEAVHAFNRMEDYGCNPDKIAFSIVISILCKKRRA 240
           KAR VEITVETFSILVRRYVRAGLAAEAVHAFNRMEDYGCNPDKIAFSIV+SILCKKRRA
Sbjct: 181 KARTVEITVETFSILVRRYVRAGLAAEAVHAFNRMEDYGCNPDKIAFSIVVSILCKKRRA 240

Query: 241 IEAQSFFDSLKQKFEPDVVVYTSLVHGWCRAGDISEAERVFKEMKMAGISPNVYTYSIVI 300
            EAQSFFDSLK KFEPDVVVYTSLVHGWCRAG+ISEAERVFKEMKMAGISPNVYTYSIVI
Sbjct: 241 SEAQSFFDSLKHKFEPDVVVYTSLVHGWCRAGEISEAERVFKEMKMAGISPNVYTYSIVI 300

Query: 301 DALCRSGQITRAHDVFSEMIDSGCNPNSVTFNNLIRVHVKAGRTEKVLQVYNQMKRLGCS 360
           DALCR GQITRAHDVFSEMIDSGCNPNSVTFNNLIRVHVKAGRTEKVLQVYNQM+RLGC+
Sbjct: 301 DALCRCGQITRAHDVFSEMIDSGCNPNSVTFNNLIRVHVKAGRTEKVLQVYNQMRRLGCA 360

Query: 361 PDIVTYNFLIETHCRDDNLGEAIKILNSMSKKGCTPNASTFNPIFRCIAKSQDVNGAHRM 420
            DI+TYNF+IETHCRDDNLGEAIK+LNSM+KKGC+PNASTFNPIFRCIAKSQDVN AHRM
Sbjct: 361 ADIITYNFIIETHCRDDNLGEAIKVLNSMAKKGCSPNASTFNPIFRCIAKSQDVNSAHRM 420

Query: 421 FAKMKDVGCKPNTVTYNILMRMFAESKSADMIFKLKKEMDEEEVEPNVNTYRELISLYCG 480
           FAKMKD+GCK NTVTYNILMRMFAESKSADMIFKLKKEMDEEEVEPNVNTYR LISLYCG
Sbjct: 421 FAKMKDLGCKANTVTYNILMRMFAESKSADMIFKLKKEMDEEEVEPNVNTYRVLISLYCG 480

Query: 481 MGHWNNAYKFFREMIEEKCLKPSMPLYEMVLQQLRKAGQLKKHEELVDKMVERGFVSRPL 535
           MGHWNNAYKFFREMIEEKCLKPSMP+Y+MVLQ LR AGQLKKHEELVDKMVERGF SRPL
Sbjct: 481 MGHWNNAYKFFREMIEEKCLKPSMPIYQMVLQLLRNAGQLKKHEELVDKMVERGFASRPL 540

BLAST of Lsi04G017760 vs. NCBI nr
Match: XP_022941057.1 (pentatricopeptide repeat-containing protein At1g20300, mitochondrial [Cucurbita moschata])

HSP 1 Score: 978.0 bits (2527), Expect = 3.2e-281
Identity = 479/534 (89.70%), Postives = 505/534 (94.57%), Query Frame = 0

Query: 1   MALIRSKPNLPLFLSYLSQRIQNHRSFSSSLSISESRLQDEPASDCPQNATNPVLSPEEI 60
           MAL +SKPNLP FLS LS  +QNHR FSSS SISES+LQDE AS+    A+ PVL PEEI
Sbjct: 1   MALFKSKPNLPPFLSSLSPFLQNHRLFSSSTSISESQLQDEDASNTTPKASKPVLLPEEI 60

Query: 61  QAADKFHALIKEYYRRNPNPDPTPPCPNFTISALSNDLSQISALHSVSPAVVRYVIEKSG 120
           QAADKFH+LIKEYYRRNP+PDPTPP PNFTISALSN+LSQIS +H+VSP VVRYVIEKSG
Sbjct: 61  QAADKFHSLIKEYYRRNPSPDPTPPSPNFTISALSNELSQISPVHAVSPGVVRYVIEKSG 120

Query: 121 GVRHGIPFLQALAFFNWATAGEGFEHSAQPYNEMIDLAGKVKQFGLAWYLIDLMKARNVE 180
           GVRHGIPFLQALAFFNWATA E FEHSAQPYNEMIDLAGKVKQFGLAW+LIDLMKARNVE
Sbjct: 121 GVRHGIPFLQALAFFNWATASETFEHSAQPYNEMIDLAGKVKQFGLAWHLIDLMKARNVE 180

Query: 181 ITVETFSILVRRYVRAGLAAEAVHAFNRMEDYGCNPDKIAFSIVISILCKKRRAIEAQSF 240
           ITVETFSILVRRYVRAGLAAEAVHAFNRMEDYGCNPDKIAF+IVISILCKKRRA EAQSF
Sbjct: 181 ITVETFSILVRRYVRAGLAAEAVHAFNRMEDYGCNPDKIAFTIVISILCKKRRATEAQSF 240

Query: 241 FDSLKQKFEPDVVVYTSLVHGWCRAGDISEAERVFKEMKMAGISPNVYTYSIVIDALCRS 300
           FD+LK KFEPDV+VYT+LVHGWCRAGDISEAERVFKEMKMAGISPNVYTYSIVIDALCR 
Sbjct: 241 FDNLKHKFEPDVIVYTNLVHGWCRAGDISEAERVFKEMKMAGISPNVYTYSIVIDALCRC 300

Query: 301 GQITRAHDVFSEMIDSGCNPNSVTFNNLIRVHVKAGRTEKVLQVYNQMKRLGCSPDIVTY 360
           GQITRAHDVFSEMID+GCNPNSVTFNNLIRVHVKAGRTEKVLQVYNQMKRLGC+ DI+TY
Sbjct: 301 GQITRAHDVFSEMIDAGCNPNSVTFNNLIRVHVKAGRTEKVLQVYNQMKRLGCAADIITY 360

Query: 361 NFLIETHCRDDNLGEAIKILNSMSKKGCTPNASTFNPIFRCIAKSQDVNGAHRMFAKMKD 420
           NFLIETHC+DDNLGEA K+LNSM+KKGCTPNASTFNPIFRCI KS DVNG HRMFAKMKD
Sbjct: 361 NFLIETHCKDDNLGEATKVLNSMAKKGCTPNASTFNPIFRCIVKSHDVNGVHRMFAKMKD 420

Query: 421 VGCKPNTVTYNILMRMFAESKSADMIFKLKKEMDEEEVEPNVNTYRELISLYCGMGHWNN 480
           +GCKPNTVTYNILMRMFAESKSADMIFKLKKEM+EEEVEPN+NTY+ LISLYCGMGHWNN
Sbjct: 421 LGCKPNTVTYNILMRMFAESKSADMIFKLKKEMEEEEVEPNLNTYQVLISLYCGMGHWNN 480

Query: 481 AYKFFREMIEEKCLKPSMPLYEMVLQQLRKAGQLKKHEELVDKMVERGFVSRPL 535
           AYKFFREMIEEKCLKPSMP+YEMVLQQLRKAGQLKKHEELVDKMVERGF SRPL
Sbjct: 481 AYKFFREMIEEKCLKPSMPIYEMVLQQLRKAGQLKKHEELVDKMVERGFASRPL 534

BLAST of Lsi04G017760 vs. NCBI nr
Match: KAG6608612.1 (Pentatricopeptide repeat-containing protein, mitochondrial, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 976.5 bits (2523), Expect = 9.4e-281
Identity = 478/534 (89.51%), Postives = 505/534 (94.57%), Query Frame = 0

Query: 1   MALIRSKPNLPLFLSYLSQRIQNHRSFSSSLSISESRLQDEPASDCPQNATNPVLSPEEI 60
           MAL +SKPNLP FLS LS  +QNHR FSSS SISES+LQDE A +   +A+ PVLSPEEI
Sbjct: 1   MALFKSKPNLPPFLSSLSPFLQNHRLFSSSTSISESQLQDEDAGNTTPHASKPVLSPEEI 60

Query: 61  QAADKFHALIKEYYRRNPNPDPTPPCPNFTISALSNDLSQISALHSVSPAVVRYVIEKSG 120
           QAADKFH+LIKEYYRRNP+PDPTPP PNF ISALSN+LSQIS +H+VSPAVVRYVIEKSG
Sbjct: 61  QAADKFHSLIKEYYRRNPSPDPTPPSPNFIISALSNELSQISPVHAVSPAVVRYVIEKSG 120

Query: 121 GVRHGIPFLQALAFFNWATAGEGFEHSAQPYNEMIDLAGKVKQFGLAWYLIDLMKARNVE 180
           GVRHGIPFLQALAFFNWATA E FEHSA PYNEMIDLAGKVKQFGLAW+LIDLMKARNVE
Sbjct: 121 GVRHGIPFLQALAFFNWATASETFEHSAHPYNEMIDLAGKVKQFGLAWHLIDLMKARNVE 180

Query: 181 ITVETFSILVRRYVRAGLAAEAVHAFNRMEDYGCNPDKIAFSIVISILCKKRRAIEAQSF 240
           ITVETFSILVRRYVRAGLAAEAVHAFNRMEDYGCNPDKIAF+IVISILCKKRRA EAQSF
Sbjct: 181 ITVETFSILVRRYVRAGLAAEAVHAFNRMEDYGCNPDKIAFTIVISILCKKRRATEAQSF 240

Query: 241 FDSLKQKFEPDVVVYTSLVHGWCRAGDISEAERVFKEMKMAGISPNVYTYSIVIDALCRS 300
           FD+LK KFEPDV+VYT+LVHGWCRAGDISEAERVFKEMKMAGISPNVYTYSIVIDALCR 
Sbjct: 241 FDNLKHKFEPDVIVYTNLVHGWCRAGDISEAERVFKEMKMAGISPNVYTYSIVIDALCRC 300

Query: 301 GQITRAHDVFSEMIDSGCNPNSVTFNNLIRVHVKAGRTEKVLQVYNQMKRLGCSPDIVTY 360
           GQITRAHDVFSEMID+GCNPNSVTFNNLIRVHVKAGRTEKVLQVYNQMKRLGC+ DI+TY
Sbjct: 301 GQITRAHDVFSEMIDAGCNPNSVTFNNLIRVHVKAGRTEKVLQVYNQMKRLGCAADIITY 360

Query: 361 NFLIETHCRDDNLGEAIKILNSMSKKGCTPNASTFNPIFRCIAKSQDVNGAHRMFAKMKD 420
           NFLIETHC+DDNLGEA K+LNSM+KKGCTPNASTFNPIFRCI KS DVNG HRMFAKMKD
Sbjct: 361 NFLIETHCKDDNLGEATKVLNSMAKKGCTPNASTFNPIFRCIVKSHDVNGVHRMFAKMKD 420

Query: 421 VGCKPNTVTYNILMRMFAESKSADMIFKLKKEMDEEEVEPNVNTYRELISLYCGMGHWNN 480
           +GCKPNTVTYNILMRMFAESKSADMIFKLKKEM+EEEVEPN+NTY+ LISLYCGMGHWNN
Sbjct: 421 LGCKPNTVTYNILMRMFAESKSADMIFKLKKEMEEEEVEPNLNTYQVLISLYCGMGHWNN 480

Query: 481 AYKFFREMIEEKCLKPSMPLYEMVLQQLRKAGQLKKHEELVDKMVERGFVSRPL 535
           AYKFFREMIEEKCLKPSMP+YEMVLQQLRKAGQLKKHEELVDKMVERGF SRPL
Sbjct: 481 AYKFFREMIEEKCLKPSMPIYEMVLQQLRKAGQLKKHEELVDKMVERGFASRPL 534

BLAST of Lsi04G017760 vs. NCBI nr
Match: XP_023524986.1 (pentatricopeptide repeat-containing protein At1g20300, mitochondrial [Cucurbita pepo subsp. pepo])

HSP 1 Score: 969.1 bits (2504), Expect = 1.5e-278
Identity = 477/534 (89.33%), Postives = 502/534 (94.01%), Query Frame = 0

Query: 1   MALIRSKPNLPLFLSYLSQRIQNHRSFSSSLSISESRLQDEPASDCPQNATNPVLSPEEI 60
           MAL +SKPNLP FLS LS  +QNHR FSSS SISES+LQD  AS+    A+ PVL PEEI
Sbjct: 1   MALFKSKPNLPPFLSSLSPFLQNHRLFSSSTSISESQLQDNDASNTTPKASKPVLLPEEI 60

Query: 61  QAADKFHALIKEYYRRNPNPDPTPPCPNFTISALSNDLSQISALHSVSPAVVRYVIEKSG 120
           QAADKF +LIKEYYRRNP+PDPTPP PNFTISALSN+LSQIS +  VSPAVVRYVIEKSG
Sbjct: 61  QAADKFLSLIKEYYRRNPSPDPTPPSPNFTISALSNELSQISPVQVVSPAVVRYVIEKSG 120

Query: 121 GVRHGIPFLQALAFFNWATAGEGFEHSAQPYNEMIDLAGKVKQFGLAWYLIDLMKARNVE 180
           GVRHGIPFLQALAFFNWATA E FEHSAQPYNEMIDLAGKVKQFGLAW+LIDLMKARNVE
Sbjct: 121 GVRHGIPFLQALAFFNWATASETFEHSAQPYNEMIDLAGKVKQFGLAWHLIDLMKARNVE 180

Query: 181 ITVETFSILVRRYVRAGLAAEAVHAFNRMEDYGCNPDKIAFSIVISILCKKRRAIEAQSF 240
           ITVETFSILVRRYVRAGLAAEAVHAFNRMEDYGCNPDKIAF+IVISILCKKRRA EAQSF
Sbjct: 181 ITVETFSILVRRYVRAGLAAEAVHAFNRMEDYGCNPDKIAFTIVISILCKKRRATEAQSF 240

Query: 241 FDSLKQKFEPDVVVYTSLVHGWCRAGDISEAERVFKEMKMAGISPNVYTYSIVIDALCRS 300
           FD+LK KFEPDV+VYT+LVHGWCRAGDISEAERVFKEMKMAGISPNVYTYSIVIDALCR 
Sbjct: 241 FDNLKHKFEPDVIVYTNLVHGWCRAGDISEAERVFKEMKMAGISPNVYTYSIVIDALCRC 300

Query: 301 GQITRAHDVFSEMIDSGCNPNSVTFNNLIRVHVKAGRTEKVLQVYNQMKRLGCSPDIVTY 360
           GQITRAHDVFSEMID+GCNPNSVTFNNLIRVHVKAGRTEKVLQVYNQMKRLGC+ DI+TY
Sbjct: 301 GQITRAHDVFSEMIDAGCNPNSVTFNNLIRVHVKAGRTEKVLQVYNQMKRLGCAADIITY 360

Query: 361 NFLIETHCRDDNLGEAIKILNSMSKKGCTPNASTFNPIFRCIAKSQDVNGAHRMFAKMKD 420
           NFLIETHC+DDNLGEA K+LNSM+KKGCTPNASTFNPIFRCI KS DVNG HRMFAKMKD
Sbjct: 361 NFLIETHCKDDNLGEATKVLNSMAKKGCTPNASTFNPIFRCIVKSHDVNGVHRMFAKMKD 420

Query: 421 VGCKPNTVTYNILMRMFAESKSADMIFKLKKEMDEEEVEPNVNTYRELISLYCGMGHWNN 480
           +GCKPNTVTYNILMRMFAESKSADMIFKLKKEM+EEEVEPN+NTY+ LISLYCGMGHWNN
Sbjct: 421 LGCKPNTVTYNILMRMFAESKSADMIFKLKKEMEEEEVEPNLNTYQVLISLYCGMGHWNN 480

Query: 481 AYKFFREMIEEKCLKPSMPLYEMVLQQLRKAGQLKKHEELVDKMVERGFVSRPL 535
           AYKFFREMIEEKCLKPSMP+YEMVLQQLRKAGQLKKHEELVDKMVERGF SRPL
Sbjct: 481 AYKFFREMIEEKCLKPSMPIYEMVLQQLRKAGQLKKHEELVDKMVERGFASRPL 534

BLAST of Lsi04G017760 vs. TAIR 10
Match: AT1G20300.1 (Pentatricopeptide repeat (PPR) superfamily protein )

HSP 1 Score: 743.0 bits (1917), Expect = 1.6e-214
Identity = 365/539 (67.72%), Postives = 447/539 (82.93%), Query Frame = 0

Query: 1   MALIRSKPNLPLFLSYLSQRIQNHRSFSSSLSISESRLQDEPASDCPQNAT---NPVLSP 60
           MAL+RSK +L   LS++S  +    S S++  +S+    DE A+      +   +P+L+P
Sbjct: 1   MALLRSKLHLSRTLSFISPLLPKTFSTSATSLLSDHE-NDESAATITAAVSVPISPLLTP 60

Query: 61  EEIQAADKFHALIKEYYRRNP-NPDPTPPCPNFTISALSNDLSQISALHSVSPAVVRYVI 120
           E+ Q  +KFH++IK++YR+NP +P+     P+ T+ ALS D SQI     VSP+VVR VI
Sbjct: 61  EDTQTVEKFHSIIKDHYRKNPTSPNDAILNPSLTLHALSLDFSQIET-SQVSPSVVRCVI 120

Query: 121 EKSGGVRHGIPFLQALAFFNWATAGEGFEH-SAQPYNEMIDLAGKVKQFGLAWYLIDLMK 180
           EK G VRHGIP  Q+LAFFNWAT+ + ++H S  PYNEMIDL+GKV+QF LAW+LIDLMK
Sbjct: 121 EKCGSVRHGIPLHQSLAFFNWATSRDDYDHKSPHPYNEMIDLSGKVRQFDLAWHLIDLMK 180

Query: 181 ARNVEITVETFSILVRRYVRAGLAAEAVHAFNRMEDYGCNPDKIAFSIVISILCKKRRAI 240
           +RNVEI++ETF+IL+RRYVRAGLA+EAVH FNRMEDYGC PDKIAFSIVIS L +KRRA 
Sbjct: 181 SRNVEISIETFTILIRRYVRAGLASEAVHCFNRMEDYGCVPDKIAFSIVISNLSRKRRAS 240

Query: 241 EAQSFFDSLKQKFEPDVVVYTSLVHGWCRAGDISEAERVFKEMKMAGISPNVYTYSIVID 300
           EAQSFFDSLK +FEPDV+VYT+LV GWCRAG+ISEAE+VFKEMK+AGI PNVYTYSIVID
Sbjct: 241 EAQSFFDSLKDRFEPDVIVYTNLVRGWCRAGEISEAEKVFKEMKLAGIEPNVYTYSIVID 300

Query: 301 ALCRSGQITRAHDVFSEMIDSGCNPNSVTFNNLIRVHVKAGRTEKVLQVYNQMKRLGCSP 360
           ALCR GQI+RAHDVF++M+DSGC PN++TFNNL+RVHVKAGRTEKVLQVYNQMK+LGC P
Sbjct: 301 ALCRCGQISRAHDVFADMLDSGCAPNAITFNNLMRVHVKAGRTEKVLQVYNQMKKLGCEP 360

Query: 361 DIVTYNFLIETHCRDDNLGEAIKILNSMSKKGCTPNASTFNPIFRCIAKSQDVNGAHRMF 420
           D +TYNFLIE HCRD+NL  A+K+LN+M KK C  NASTFN IFR I K +DVNGAHRM+
Sbjct: 361 DTITYNFLIEAHCRDENLENAVKVLNTMIKKKCEVNASTFNTIFRYIEKKRDVNGAHRMY 420

Query: 421 AKMKDVGCKPNTVTYNILMRMFAESKSADMIFKLKKEMDEEEVEPNVNTYRELISLYCGM 480
           +KM +  C+PNTVTYNILMRMF  SKS DM+ K+KKEMD++EVEPNVNTYR L++++CGM
Sbjct: 421 SKMMEAKCEPNTVTYNILMRMFVGSKSTDMVLKMKKEMDDKEVEPNVNTYRLLVTMFCGM 480

Query: 481 GHWNNAYKFFREMIEEKCLKPSMPLYEMVLQQLRKAGQLKKHEELVDKMVERGFVSRPL 535
           GHWNNAYK F+EM+EEKCL PS+ LYEMVL QLR+AGQLKKHEELV+KM+++G V+RPL
Sbjct: 481 GHWNNAYKLFKEMVEEKCLTPSLSLYEMVLAQLRRAGQLKKHEELVEKMIQKGLVARPL 537

BLAST of Lsi04G017760 vs. TAIR 10
Match: AT1G71060.1 (Tetratricopeptide repeat (TPR)-like superfamily protein )

HSP 1 Score: 237.3 bits (604), Expect = 2.9e-62
Identity = 140/437 (32.04%), Postives = 232/437 (53.09%), Query Frame = 0

Query: 89  FTISALSNDLSQISALHSVSPAVVRYVIEKSGGVRHGIPFLQALAFFNWATAGEGFEHSA 148
           FT S +   L++ S    +SPA++  V++K          + AL+ F WA   +GF+H+ 
Sbjct: 76  FTDSKVETLLNEASV--KLSPALIEEVLKKLSNAG-----VLALSVFKWAENQKGFKHTT 135

Query: 149 QPYNEMIDLAGKVKQFGLAWYLIDLMKARNVEITVETFSILVRRYVRAGLAAEAVHAFNR 208
             YN +I+  GK+KQF L W L+D MKA+ + ++ ETF+++ RRY RA    EA+ AF++
Sbjct: 136 SNYNALIESLGKIKQFKLIWSLVDDMKAKKL-LSKETFALISRRYARARKVKEAIGAFHK 195

Query: 209 MEDYGCNPDKIAFSIVISILCKKRRAIEAQSFFDSLKQK-FEPDVVVYTSLVHGWCRAGD 268
           ME++G   +   F+ ++  L K R   +AQ  FD +K+K FEPD+  YT L+ GW +  +
Sbjct: 196 MEEFGFKMESSDFNRMLDTLSKSRNVGDAQKVFDKMKKKRFEPDIKSYTILLEGWGQELN 255

Query: 269 ISEAERVFKEMKMAGISPNVYTYSIVIDALCRSGQITRAHDVFSEMIDSGCNPNSVTFNN 328
           +   + V +EMK  G  P+V  Y I+I+A C++ +   A   F+EM    C P+   F +
Sbjct: 256 LLRVDEVNREMKDEGFEPDVVAYGIIINAHCKAKKYEEAIRFFNEMEQRNCKPSPHIFCS 315

Query: 329 LIRVHVKAGRTEKVLQVYNQMKRLGCSPDIVTYNFLIETHCRDDNLGEAIKILNSMSKKG 388
           LI       +    L+ + + K  G   +  TYN L+  +C    + +A K ++ M  KG
Sbjct: 316 LINGLGSEKKLNDALEFFERSKSSGFPLEAPTYNALVGAYCWSQRMEDAYKTVDEMRLKG 375

Query: 389 CTPNASTFNPIFRCIAKSQDVNGAHRMFAKMKDVGCKPNTVTYNILMRMFAESKSADMIF 448
             PNA T++ I   + + Q    A+ ++  M    C+P   TY I++RMF   +  DM  
Sbjct: 376 VGPNARTYDIILHHLIRMQRSKEAYEVYQTM---SCEPTVSTYEIMVRMFCNKERLDMAI 435

Query: 449 KLKKEMDEEEVEPNVNTYRELISLYCGMGHWNNAYKFFREMIEEKCLKPSMPLYEMVLQQ 508
           K+  EM  + V P ++ +  LI+  C     + A ++F EM++   ++P   ++  + Q 
Sbjct: 436 KIWDEMKGKGVLPGMHMFSSLITALCHENKLDEACEYFNEMLDVG-IRPPGHMFSRLKQT 495

Query: 509 LRKAGQLKKHEELVDKM 525
           L   G+  K  +LV KM
Sbjct: 496 LLDEGRKDKVTDLVVKM 500

BLAST of Lsi04G017760 vs. TAIR 10
Match: AT1G77360.1 (Tetratricopeptide repeat (TPR)-like superfamily protein )

HSP 1 Score: 235.7 bits (600), Expect = 8.5e-62
Identity = 124/377 (32.89%), Postives = 213/377 (56.50%), Query Frame = 0

Query: 134 FFNWATAGEGFEHSAQPYNEMIDLAGKVKQFGLAWYLIDLMKARNVEITVETFSILVRRY 193
           FF W+     +EHS + Y+ MI+   K++Q+ L W LI+ M+ + + + VETF I++R+Y
Sbjct: 120 FFQWSEKQRHYEHSVRAYHMMIESTAKIRQYKLMWDLINAMRKKKM-LNVETFCIVMRKY 179

Query: 194 VRAGLAAEAVHAFNRMEDYGCNPDKIAFSIVISILCKKRRAIEAQSFFDSLKQKFEPDVV 253
            RA    EA++AFN ME Y   P+ +AF+ ++S LCK +   +AQ  F++++ +F PD  
Sbjct: 180 ARAQKVDEAIYAFNVMEKYDLPPNLVAFNGLLSALCKSKNVRKAQEVFENMRDRFTPDSK 239

Query: 254 VYTSLVHGWCRAGDISEAERVFKEMKMAGISPNVYTYSIVIDALCRSGQITRAHDVFSEM 313
            Y+ L+ GW +  ++ +A  VF+EM  AG  P++ TYSI++D LC++G++  A  +   M
Sbjct: 240 TYSILLEGWGKEPNLPKAREVFREMIDAGCHPDIVTYSIMVDILCKAGRVDEALGIVRSM 299

Query: 314 IDSGCNPNSVTFNNLIRVHVKAGRTEKVLQVYNQMKRLGCSPDIVTYNFLIETHCRDDNL 373
             S C P +  ++ L+  +    R E+ +  + +M+R G   D+  +N LI   C+ + +
Sbjct: 300 DPSICKPTTFIYSVLVHTYGTENRLEEAVDTFLEMERSGMKADVAVFNSLIGAFCKANRM 359

Query: 374 GEAIKILNSMSKKGCTPNASTFNPIFRCIAKSQDVNGAHRMFAKMKDVGCKPNTVTYNIL 433
               ++L  M  KG TPN+ + N I R + +  + + A  +F KM  V C+P+  TY ++
Sbjct: 360 KNVYRVLKEMKSKGVTPNSKSCNIILRHLIERGEKDEAFDVFRKMIKV-CEPDADTYTMV 419

Query: 434 MRMFAESKSADMIFKLKKEMDEEEVEPNVNTYRELISLYCGMGHWNNAYKFFREMIEEKC 493
           ++MF E K  +   K+ K M ++ V P+++T+  LI+  C       A     EMI E  
Sbjct: 420 IKMFCEKKEMETADKVWKYMRKKGVFPSMHTFSVLINGLCEERTTQKACVLLEEMI-EMG 479

Query: 494 LKPSMPLYEMVLQQLRK 511
           ++PS   +  + Q L K
Sbjct: 480 IRPSGVTFGRLRQLLIK 493

BLAST of Lsi04G017760 vs. TAIR 10
Match: AT3G49730.1 (Tetratricopeptide repeat (TPR)-like superfamily protein )

HSP 1 Score: 221.5 bits (563), Expect = 1.7e-57
Identity = 112/358 (31.28%), Postives = 191/358 (53.35%), Query Frame = 0

Query: 134 FFNWATAGEGFEHSAQPYNEMIDLAGKVKQFGLAWYLIDLMKARNVE-ITVETFSILVRR 193
           FF WAT   G+ HS +    M+ +  K++QFG  W LI+ M+  N E I  E F +L+RR
Sbjct: 117 FFLWATKQPGYFHSYEVCKSMVMILSKMRQFGAVWGLIEEMRKTNPELIEPELFVVLMRR 176

Query: 194 YVRAGLAAEAVHAFNRMEDYGCNPDKIAFSIVISILCKKRRAIEAQSFFDSLKQKFEPDV 253
           +  A +  +AV   + M  YG  PD+  F  ++  LCK     EA   F+ +++KF P++
Sbjct: 177 FASANMVKKAVEVLDEMPKYGLEPDEYVFGCLLDALCKNGSVKEASKVFEDMREKFPPNL 236

Query: 254 VVYTSLVHGWCRAGDISEAERVFKEMKMAGISPNVYTYSIVIDALCRSGQITRAHDVFSE 313
             +TSL++GWCR G + EA+ V  +MK AG+ P++  ++ ++     +G++  A+D+ ++
Sbjct: 237 RYFTSLLYGWCREGKLMEAKEVLVQMKEAGLEPDIVVFTNLLSGYAHAGKMADAYDLMND 296

Query: 314 MIDSGCNPNSVTFNNLIRVHVKA-GRTEKVLQVYNQMKRLGCSPDIVTYNFLIETHCRDD 373
           M   G  PN   +  LI+   +   R ++ ++V+ +M+R GC  DIVTY  LI   C+  
Sbjct: 297 MRKRGFEPNVNCYTVLIQALCRTEKRMDEAMRVFVEMERYGCEADIVTYTALISGFCKWG 356

Query: 374 NLGEAIKILNSMSKKGCTPNASTFNPIFRCIAKSQDVNGAHRMFAKMKDVGCKPNTVTYN 433
            + +   +L+ M KKG  P+  T+  I     K +       +  KMK  GC P+ + YN
Sbjct: 357 MIDKGYSVLDDMRKKGVMPSQVTYMQIMVAHEKKEQFEECLELIEKMKRRGCHPDLLIYN 416

Query: 434 ILMRMFAESKSADMIFKLKKEMDEEEVEPNVNTYRELISLYCGMGHWNNAYKFFREMI 490
           +++R+  +        +L  EM+   + P V+T+  +I+ +   G    A   F+EM+
Sbjct: 417 VVIRLACKLGEVKEAVRLWNEMEANGLSPGVDTFVIMINGFTSQGFLIEACNHFKEMV 474

BLAST of Lsi04G017760 vs. TAIR 10
Match: AT1G74900.1 (Pentatricopeptide repeat (PPR) superfamily protein )

HSP 1 Score: 210.3 bits (534), Expect = 3.8e-54
Identity = 113/384 (29.43%), Postives = 202/384 (52.60%), Query Frame = 0

Query: 130 QALAFFNWA-TAGEGFEHSAQPYNEMIDLAGKVKQFGLAWYLIDLMKARNVEITVETFSI 189
           +AL FF++       + H A  ++  ID+A ++      W LI  M++  +  + +TF+I
Sbjct: 72  KALQFFHFLDNHHREYVHDASSFDLAIDIAARLHLHPTVWSLIHRMRSLRIGPSPKTFAI 131

Query: 190 LVRRYVRAGLAAEAVHAFNRMEDYGCNPDKIAFSIVISILCKKRRAIEAQSFFDSLKQKF 249
           +  RY  AG   +AV  F  M ++GC  D  +F+ ++ +LCK +R  +A   F +L+ +F
Sbjct: 132 VAERYASAGKPDKAVKLFLNMHEHGCFQDLASFNTILDVLCKSKRVEKAYELFRALRGRF 191

Query: 250 EPDVVVYTSLVHGWCRAGDISEAERVFKEMKMAGISPNVYTYSIVIDALCRSGQITRAHD 309
             D V Y  +++GWC      +A  V KEM   GI+PN+ TY+ ++    R+GQI  A +
Sbjct: 192 SVDTVTYNVILNGWCLIKRTPKALEVLKEMVERGINPNLTTYNTMLKGFFRAGQIRHAWE 251

Query: 310 VFSEMIDSGCNPNSVTFNNLIRVHVKAGRTEKVLQVYNQMKRLGCSPDIVTYNFLIETHC 369
            F EM    C  + VT+  ++     AG  ++   V+++M R G  P + TYN +I+  C
Sbjct: 252 FFLEMKKRDCEIDVVTYTTVVHGFGVAGEIKRARNVFDEMIREGVLPSVATYNAMIQVLC 311

Query: 370 RDDNLGEAIKILNSMSKKGCTPNASTFNPIFRCIAKSQDVNGAHRMFAKMKDVGCKPNTV 429
           + DN+  A+ +   M ++G  PN +T+N + R +  + + +    +  +M++ GC+PN  
Sbjct: 312 KKDNVENAVVMFEEMVRRGYEPNVTTYNVLIRGLFHAGEFSRGEELMQRMENEGCEPNFQ 371

Query: 430 TYNILMRMFAESKSADMIFKLKKEMDEEEVEPNVNTYRELISLYCGMGHWNNAYKFFREM 489
           TYN+++R ++E    +    L ++M   +  PN++TY  LIS   GM        F R+ 
Sbjct: 372 TYNMMIRYYSECSEVEKALGLFEKMGSGDCLPNLDTYNILIS---GM--------FVRKR 431

Query: 490 IEEKCLKPSMPLYEMVLQQLRKAG 513
            E+  +  +    + +L+   K+G
Sbjct: 432 SEDMVVAGNQAFAKEILRLQSKSG 444

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LN222.3e-21367.72Pentatricopeptide repeat-containing protein At1g20300, mitochondrial OS=Arabidop... [more]
Q9C9A24.1e-6132.04Pentatricopeptide repeat-containing protein At1g71060, mitochondrial OS=Arabidop... [more]
Q9FVX21.2e-6032.89Pentatricopeptide repeat-containing protein At1g77360, mitochondrial OS=Arabidop... [more]
P0C8A02.3e-5631.28Pentatricopeptide repeat-containing protein At3g49730 OS=Arabidopsis thaliana OX... [more]
Q9S7R43.1e-5330.70Pentatricopeptide repeat-containing protein At1g74900, mitochondrial OS=Arabidop... [more]
Match NameE-valueIdentityDescription
A0A6J1CG384.9e-28389.81pentatricopeptide repeat-containing protein At1g20300, mitochondrial isoform X1 ... [more]
A0A6J1FLC31.6e-28189.70pentatricopeptide repeat-containing protein At1g20300, mitochondrial OS=Cucurbit... [more]
A0A6J1J1A01.2e-27888.95pentatricopeptide repeat-containing protein At1g20300, mitochondrial OS=Cucurbit... [more]
A0A5A7V5E79.2e-27487.64Calcium-dependent protein kinase 29 OS=Cucumis melo var. makuwa OX=1194695 GN=E6... [more]
A0A0A0LE951.6e-27387.08PPR_long domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G874400 PE... [more]
Match NameE-valueIdentityDescription
XP_038877723.11.2e-28892.86pentatricopeptide repeat-containing protein At1g20300, mitochondrial [Benincasa ... [more]
XP_022140589.11.0e-28289.81pentatricopeptide repeat-containing protein At1g20300, mitochondrial isoform X1 ... [more]
XP_022941057.13.2e-28189.70pentatricopeptide repeat-containing protein At1g20300, mitochondrial [Cucurbita ... [more]
KAG6608612.19.4e-28189.51Pentatricopeptide repeat-containing protein, mitochondrial, partial [Cucurbita a... [more]
XP_023524986.11.5e-27889.33pentatricopeptide repeat-containing protein At1g20300, mitochondrial [Cucurbita ... [more]
Match NameE-valueIdentityDescription
AT1G20300.11.6e-21467.72Pentatricopeptide repeat (PPR) superfamily protein [more]
AT1G71060.12.9e-6232.04Tetratricopeptide repeat (TPR)-like superfamily protein [more]
AT1G77360.18.5e-6232.89Tetratricopeptide repeat (TPR)-like superfamily protein [more]
AT3G49730.11.7e-5731.28Tetratricopeptide repeat (TPR)-like superfamily protein [more]
AT1G74900.13.8e-5429.43Pentatricopeptide repeat (PPR) superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002885Pentatricopeptide repeatPFAMPF13812PPR_3coord: 448..505
e-value: 1.4E-6
score: 28.3
coord: 171..228
e-value: 1.5E-4
score: 21.8
coord: 378..437
e-value: 3.0E-11
score: 43.2
IPR002885Pentatricopeptide repeatTIGRFAMTIGR00756TIGR00756coord: 288..321
e-value: 8.2E-11
score: 39.4
coord: 185..217
e-value: 3.6E-6
score: 24.8
coord: 151..182
e-value: 0.0024
score: 15.9
coord: 394..427
e-value: 8.5E-6
score: 23.6
coord: 428..462
e-value: 6.2E-5
score: 20.9
coord: 253..287
e-value: 1.5E-10
score: 38.6
coord: 464..494
e-value: 9.7E-5
score: 20.3
coord: 358..392
e-value: 8.0E-8
score: 30.0
coord: 323..357
e-value: 1.1E-10
score: 38.9
IPR002885Pentatricopeptide repeatPFAMPF13041PPR_2coord: 250..299
e-value: 3.0E-19
score: 68.9
coord: 320..369
e-value: 1.6E-15
score: 57.0
IPR002885Pentatricopeptide repeatPROSITEPS51375PPRcoord: 391..425
score: 10.39137
IPR002885Pentatricopeptide repeatPROSITEPS51375PPRcoord: 461..495
score: 10.435215
IPR002885Pentatricopeptide repeatPROSITEPS51375PPRcoord: 426..460
score: 10.106377
IPR002885Pentatricopeptide repeatPROSITEPS51375PPRcoord: 251..285
score: 14.085328
IPR002885Pentatricopeptide repeatPROSITEPS51375PPRcoord: 182..216
score: 10.676364
IPR002885Pentatricopeptide repeatPROSITEPS51375PPRcoord: 286..320
score: 13.526301
IPR002885Pentatricopeptide repeatPROSITEPS51375PPRcoord: 356..390
score: 12.210946
IPR002885Pentatricopeptide repeatPROSITEPS51375PPRcoord: 321..355
score: 12.704205
IPR011990Tetratricopeptide-like helical domain superfamilyGENE3D1.25.40.10Tetratricopeptide repeat domaincoord: 122..233
e-value: 2.8E-22
score: 80.9
coord: 234..333
e-value: 1.2E-34
score: 121.3
IPR011990Tetratricopeptide-like helical domain superfamilyGENE3D1.25.40.10Tetratricopeptide repeat domaincoord: 451..528
e-value: 5.3E-14
score: 54.2
IPR011990Tetratricopeptide-like helical domain superfamilyGENE3D1.25.40.10Tetratricopeptide repeat domaincoord: 334..450
e-value: 1.7E-33
score: 118.4
IPR011990Tetratricopeptide-like helical domain superfamilySUPERFAMILY48452TPR-likecoord: 217..491
NoneNo IPR availablePANTHERPTHR45613PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEINcoord: 13..533
NoneNo IPR availablePANTHERPTHR45613:SF128CHLOROPLAST RNA PROCESSING 1-LIKE PROTEINcoord: 13..533

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi04G017760.1Lsi04G017760.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006468 protein phosphorylation
molecular_function GO:0005524 ATP binding
molecular_function GO:0005509 calcium ion binding
molecular_function GO:0005515 protein binding
molecular_function GO:0004672 protein kinase activity