Lsi04G016100 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi04G016100
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	cytochrome P450 82A3-like
Location	chr04: 23614313 .. 23616596 (+)
RNA-Seq Expression	Lsi04G016100
Synteny	Lsi04G016100

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

CGAAGACTTACCTAGCTTTTTCTTCTTCTGTGGTTGATATTAAATTCTCGCTTTTCTTGTCCATCCATTATAAAATCTTTAAAGGGAATCCAAAAATTATGGAATTCCAATTTCCCTCCTTTAACCCAATATCCATCACGATGCTTGTCGTTTTGTTTTTTATTTCATGTTATTACCTGTGGCGAAAGTGGGCGCAGCATAAAGTTACTGAGCAGAAACAGGCCCCGGCGCCGCCGGGAGCGTGGCCTGTAATCGGCCACCTCCATCTGCTGCGCAATGCGAAGCTTCCCCATCATGCTCTTGGAGCCATGGCCGACAAATATGGGCCGATTTTCCGGCTGCAGCTGGGATCACGGTCGGCTCTGGTGGTGAGCAGCTGGGAAATGGCGAAGGAAAGCATGTGCGTCAACGATGCGGCCGCCGCCTCCCGCCCTGGAGTCTCCGGCACCAAATATTTCTCTTATGACTTTGCAGCCTTTGGGTTGGCTCCTTACAGTCCTTACTGGCGGGAGATTCGTAAAGTTACTCATATGGAGCTCCTCTCTAATCCACGCGTTGATCAGGTTTTAAAACTACCCCACTTTACCCAAAGCTTAAACAAATTTGTAAAAAAAAAAAAAAAAAAAAAAAAGGTGTTTGGTTGGGTATTTTATTGTTGTTATTAATATTGAGAAAGACAGGTTAAGAGCATCATGTTTGGTGAGATCAACACATCATTGACAGAGCTATACGAAACATGGGAGGCCGAAAAGGAGGAATCAGGGCAGGTTCAAGTGGAAATGAAGCGTTGGTTTGGGAATGTGATTGTCAATATGCTTCTTAAAATCATAATCGGAAAACGATGTGTCGGTCCGAACGCCGAGGGAGGTGAAAAACAAGCTAAAGATTTTCAGTTGGCAATTAGGGACTCATTTCATTTAATGGGTCAAGGGTTATTGAGAGACTATATTCCGATCATCGGACGGCTGGGATTTAATGGACAAGTGAAGGTAATGAAGAATATAGCCACACGATTCGATATGGTTTTAAGTGAATGGTTGGAAGAGCACAAGCGTAAGAGAGCTTCTTCTTCTTCTTTTTATGGTAAAAAAGATGGGGACTTTATGGATTCTCTCCTTTCTTTATATGATGGCAAAGAGATTGAAGGCTACTATGATGGTGATACCATTATCAAAGCCACAACCCTTGTACGTACCCTTAATTCTCTTTTATCATTAACTTTTTTTTTAGTTCGGGACAAAAATCAATTTATACTCCTAAATTTGGAGATCGGAGATAATGATATCAATTTAAAGAAGTTTTACAAATATGTGAGAATTATTCATCAACGATTTTGAAATAATTCTAATAATGGGTTAAAATTAGATTCAGTTATGAAAGTTTAGAGTTTTGATTGAGCTTTTAACTTGACAACCATTCTCAAAGTAATTTACTTTAAACCACACCAGCTTGAGTTTTTAGATTTTAACATGGAATCAGAGTATGAAATTTTAATGTTATTTTTTACTCAATTAATATTGACTTTCATCTACTGGGTCTTGTATAAAATTTTCAAGACACAAGTGAGGAAGATTTAAATTTACAGTTGTAATCCATCAACTTAAACTTTTAGATTTTGTGGTAATTTAATAGACACATATGTATGTGTGTGTAGAACATGATTGCAGGTGGAACTGAAAGTACGACGGTGACTCTAACATGGGCGATGTCATTGTTACTAAACAATCCACGAGTCCTTGAAAAGGCACAACAAGAGTTGGACACGGTTGTAGGGCGAGAGAGGCGAGTGAAGGAATCAGACATAGGCAAGCTTGTTTATTTACAATCCATAATCAAAGAAACGATGCGAATGTATCCAGCAGGGCCTTTATTGGGGCCTCGTGAGTTCTACAAGGACTGTGTTGTGGCTGGTTACTTTGTCCCCAAAGGCACCCAATTGATCCCAAACATTTGGAAGATCCAAACCGACTCACGTGTTTGGCCCGACCCATTTGAATTCAAGCCGGAGCGGTTTTTGACGAGCCATAAGGATGTGGATTTGAAAGGGAATAATTTCGAGTTGATTCCGTTTGGGAGTGGAAGGAGAGGGTGTCCTGGAATTGCTTTTGGACTTCAAATGGTGCATTTTACTTTGGCTGGATTTTTACACTCATTTGATGTAAAAAAACCGACCAAGGAACCTATTGATATGTCGGAGAATTTTGGAATGGCTAATGAGAAAGTGGTTCCTCTCAATGTTACGGTCACTTCACGGTTACCTTCTCATCTTCACGCCGTCAATTAG

mRNA sequence

CGAAGACTTACCTAGCTTTTTCTTCTTCTGTGGTTGATATTAAATTCTCGCTTTTCTTGTCCATCCATTATAAAATCTTTAAAGGGAATCCAAAAATTATGGAATTCCAATTTCCCTCCTTTAACCCAATATCCATCACGATGCTTGTCGTTTTGTTTTTTATTTCATGTTATTACCTGTGGCGAAAGTGGGCGCAGCATAAAGTTACTGAGCAGAAACAGGCCCCGGCGCCGCCGGGAGCGTGGCCTGTAATCGGCCACCTCCATCTGCTGCGCAATGCGAAGCTTCCCCATCATGCTCTTGGAGCCATGGCCGACAAATATGGGCCGATTTTCCGGCTGCAGCTGGGATCACGGTCGGCTCTGGTGGTGAGCAGCTGGGAAATGGCGAAGGAAAGCATGTGCGTCAACGATGCGGCCGCCGCCTCCCGCCCTGGAGTCTCCGGCACCAAATATTTCTCTTATGACTTTGCAGCCTTTGGGTTGGCTCCTTACAGTCCTTACTGGCGGGAGATTCGTAAAGTTACTCATATGGAGCTCCTCTCTAATCCACGCGTTGATCAGGTTAAGAGCATCATGTTTGGTGAGATCAACACATCATTGACAGAGCTATACGAAACATGGGAGGCCGAAAAGGAGGAATCAGGGCAGGTTCAAGTGGAAATGAAGCGTTGGTTTGGGAATGTGATTGTCAATATGCTTCTTAAAATCATAATCGGAAAACGATGTGTCGGTCCGAACGCCGAGGGAGGTGAAAAACAAGCTAAAGATTTTCAGTTGGCAATTAGGGACTCATTTCATTTAATGGGTCAAGGGTTATTGAGAGACTATATTCCGATCATCGGACGGCTGGGATTTAATGGACAAGTGAAGGTAATGAAGAATATAGCCACACGATTCGATATGGTTTTAAGTGAATGGTTGGAAGAGCACAAGCGGCGAGAGAGGCGAGTGAAGGAATCAGACATAGGCAAGCTTGTTTATTTACAATCCATAATCAAAGAAACGATGCGAATGTATCCAGCAGGGCCTTTATTGGGGCCTCGTGAGTTCTACAAGGACTGTGTTGTGGCTGGTTACTTTGTCCCCAAAGGCACCCAATTGATCCCAAACATTTGGAAGATCCAAACCGACTCACGTGTTTGGCCCGACCCATTTGAATTCAAGCCGGAGCGGTTTTTGACGAGCCATAAGGATGTGGATTTGAAAGGGAATAATTTCGAGTTGATTCCGTTTGGGAGTGGAAGGAGAGGGTGTCCTGGAATTGCTTTTGGACTTCAAATGGTGCATTTTACTTTGGCTGGATTTTTACACTCATTTGATGTAAAAAAACCGACCAAGGAACCTATTGATATGTCGGAGAATTTTGGAATGGCTAATGAGAAAGTGGTTCCTCTCAATGTTACGGTCACTTCACGGTTACCTTCTCATCTTCACGCCGTCAATTAG

Coding sequence (CDS)

ATGGAATTCCAATTTCCCTCCTTTAACCCAATATCCATCACGATGCTTGTCGTTTTGTTTTTTATTTCATGTTATTACCTGTGGCGAAAGTGGGCGCAGCATAAAGTTACTGAGCAGAAACAGGCCCCGGCGCCGCCGGGAGCGTGGCCTGTAATCGGCCACCTCCATCTGCTGCGCAATGCGAAGCTTCCCCATCATGCTCTTGGAGCCATGGCCGACAAATATGGGCCGATTTTCCGGCTGCAGCTGGGATCACGGTCGGCTCTGGTGGTGAGCAGCTGGGAAATGGCGAAGGAAAGCATGTGCGTCAACGATGCGGCCGCCGCCTCCCGCCCTGGAGTCTCCGGCACCAAATATTTCTCTTATGACTTTGCAGCCTTTGGGTTGGCTCCTTACAGTCCTTACTGGCGGGAGATTCGTAAAGTTACTCATATGGAGCTCCTCTCTAATCCACGCGTTGATCAGGTTAAGAGCATCATGTTTGGTGAGATCAACACATCATTGACAGAGCTATACGAAACATGGGAGGCCGAAAAGGAGGAATCAGGGCAGGTTCAAGTGGAAATGAAGCGTTGGTTTGGGAATGTGATTGTCAATATGCTTCTTAAAATCATAATCGGAAAACGATGTGTCGGTCCGAACGCCGAGGGAGGTGAAAAACAAGCTAAAGATTTTCAGTTGGCAATTAGGGACTCATTTCATTTAATGGGTCAAGGGTTATTGAGAGACTATATTCCGATCATCGGACGGCTGGGATTTAATGGACAAGTGAAGGTAATGAAGAATATAGCCACACGATTCGATATGGTTTTAAGTGAATGGTTGGAAGAGCACAAGCGGCGAGAGAGGCGAGTGAAGGAATCAGACATAGGCAAGCTTGTTTATTTACAATCCATAATCAAAGAAACGATGCGAATGTATCCAGCAGGGCCTTTATTGGGGCCTCGTGAGTTCTACAAGGACTGTGTTGTGGCTGGTTACTTTGTCCCCAAAGGCACCCAATTGATCCCAAACATTTGGAAGATCCAAACCGACTCACGTGTTTGGCCCGACCCATTTGAATTCAAGCCGGAGCGGTTTTTGACGAGCCATAAGGATGTGGATTTGAAAGGGAATAATTTCGAGTTGATTCCGTTTGGGAGTGGAAGGAGAGGGTGTCCTGGAATTGCTTTTGGACTTCAAATGGTGCATTTTACTTTGGCTGGATTTTTACACTCATTTGATGTAAAAAAACCGACCAAGGAACCTATTGATATGTCGGAGAATTTTGGAATGGCTAATGAGAAAGTGGTTCCTCTCAATGTTACGGTCACTTCACGGTTACCTTCTCATCTTCACGCCGTCAATTAG

Protein sequence

MEFQFPSFNPISITMLVVLFFISCYYLWRKWAQHKVTEQKQAPAPPGAWPVIGHLHLLRNAKLPHHALGAMADKYGPIFRLQLGSRSALVVSSWEMAKESMCVNDAAAASRPGVSGTKYFSYDFAAFGLAPYSPYWREIRKVTHMELLSNPRVDQVKSIMFGEINTSLTELYETWEAEKEESGQVQVEMKRWFGNVIVNMLLKIIIGKRCVGPNAEGGEKQAKDFQLAIRDSFHLMGQGLLRDYIPIIGRLGFNGQVKVMKNIATRFDMVLSEWLEEHKRRERRVKESDIGKLVYLQSIIKETMRMYPAGPLLGPREFYKDCVVAGYFVPKGTQLIPNIWKIQTDSRVWPDPFEFKPERFLTSHKDVDLKGNNFELIPFGSGRRGCPGIAFGLQMVHFTLAGFLHSFDVKKPTKEPIDMSENFGMANEKVVPLNVTVTSRLPSHLHAVN

Homology

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BLAST of Lsi04G016100 vs. ExPASy Swiss-Prot
Match: O49858 (Cytochrome P450 82A3 OS=Glycine max OX=3847 GN=CYP82A3 PE=2 SV=1)

HSP 1 Score: 412.1 bits (1058), Expect = 7.8e-114
Identity = 211/516 (40.89%), Postives = 290/516 (56.20%), Query Frame = 0


Query: 7   SFNPISITMLVVLFFISCYYLWRKWAQHKVTEQKQAPAPPGAWPVIGHLHLLRNAKLPHH 66
           + N I+I  ++ L F+ C +L+R     K +  K AP   GAWP++GHL LL  ++ PH 
Sbjct: 9   NLNTIAIASILSLIFL-CLFLYR-----KNSRGKDAPVVSGAWPILGHLSLLNGSQTPHK 68

Query: 67  ALGAMADKYGPIFRLQLGSRSALVVSSWEMAKESMCVNDAAAASRPGVSGTKYFSYDFAA 126
            LGA+ADKYGP+F ++LG + ALV+S+WEM+KE    ND A +SRP +   +  SY+ A 
Sbjct: 69  VLGALADKYGPLFTIKLGMKPALVLSNWEMSKELFTTNDLAVSSRPKLVAVEVMSYNQAF 128

Query: 127 FGLAPYSPYWREIRKVTHMELLSNPRVDQVKSIMFGEINTSLTELYETW-EAEKEESGQV 186
            GLAPY PYWRE+RK+   E LSN R++Q   I   E+ TS+ EL++ W    K ES   
Sbjct: 129 VGLAPYGPYWRELRKIVTFEFLSNRRIEQRNHIRVSEVRTSIKELFDIWSNGNKNESRYT 188

Query: 187 QVEMKRWFGNVIVNMLLKIIIGKRCVGPNAEGGEKQAKDFQLAIRDSFHLMGQGLLRDYI 246
            V++K+W   +  NM++++++GKR  G     G+ +A+ F   IR+  +LMG   + D +
Sbjct: 189 LVDIKQWLAYLTFNMVVRMVVGKRYFGVMHVEGKDKAQRFMKNIREFMNLMGTFTVADGV 248

Query: 247 PIIGRLGFNGQVKVMKNIATRFDMVLSEWLEEHKR------------------------- 306
           P +  L   G  K MK  A   D +LSEWLEEH++                         
Sbjct: 249 PCLRWLDLGGHEKAMKANAKEVDKLLSEWLEEHRQKKLLGENVESDRDFMDVMISALNGA 308

Query: 307 --------------------------------------------------------RERR 366
                                                                   ++  
Sbjct: 309 QIGAFDADTICKATSLELILGGTDSTAVTLTWALSLLLRNPLALGKAKEEIDMQIGKDEY 368

Query: 367 VKESDIGKLVYLQSIIKETMRMYPAGPLLGPREFYKDCVVAGYFVPKGTQLIPNIWKIQT 426
           ++ESDI KLVYLQ+I+KET+R+YP  P   PREF ++C++ GY + KGT+LI N+WKI  
Sbjct: 369 IRESDISKLVYLQAIVKETLRLYPPAPFSSPREFTENCILGGYHIKKGTRLIHNLWKIHR 428

Query: 427 DSRVWPDPFEFKPERFLTSHKDVDLKGNNFELIPFGSGRRGCPGIAFGLQMVHFTLAGFL 441
           D  VW DP EFKPERFLT+HKDVDL+G+NFEL+PFGSGRR C G++ GL MVHFTLA  L
Sbjct: 429 DPSVWSDPLEFKPERFLTTHKDVDLRGHNFELLPFGSGRRVCAGMSLGLNMVHFTLANLL 488

[Close]

BLAST of Lsi04G016100 vs. ExPASy Swiss-Prot
Match: O49859 (Cytochrome P450 82A4 OS=Glycine max OX=3847 GN=CYP82A4 PE=2 SV=1)

HSP 1 Score: 409.8 bits (1052), Expect = 3.9e-113
Identity = 206/502 (41.04%), Postives = 290/502 (57.77%), Query Frame = 0


Query: 16  LVVLFFISCYYLWRKWAQHKVTEQKQAPAPPGAWPVIGHLHLLRNAKLPHHALGAMADKY 75
           +V L F+ C +L+    +      K+AP   GAWP+ GHL LL  +K PH ALGA+A+K+
Sbjct: 15  VVSLIFLLCLFLYGP-LKKVHGSSKEAPTVGGAWPIFGHLPLLIGSKSPHKALGALAEKH 74

Query: 76  GPIFRLQLGSRSALVVSSWEMAKESMCVNDAAAASRPGVSGTKYFSYDFAAFGLAPYSPY 135
           GP+F ++LG++ ALVVS WEMA+E    ND A ++RP +   +   Y+ A   +APY PY
Sbjct: 75  GPLFTIKLGAKKALVVSDWEMARECFTTNDVAVSARPKLLVAELMCYNNAMLLVAPYGPY 134

Query: 136 WREIRKVTHMELLSNPRVDQVKSIMFGEINTSLTELYETWEAEKEESGQVQVEMKRWFGN 195
           WRE+RK+   E+LS+ RV+Q++ +   E+  S+ ELY+ W ++K ES    VE+K+WF  
Sbjct: 135 WRELRKIIVTEILSSSRVEQLQDVRVSEVQNSIVELYDVWRSQKNESDYASVELKQWFAQ 194

Query: 196 VIVNMLLKIIIGKRCVGPNAEGGEKQAKDFQLAIRDSFHLMGQGLLRDYIPIIGRLGFNG 255
            I NM+L++++GKR +  +A   +++A+    A+ +   L G   + D IP +  L F G
Sbjct: 195 PIFNMVLRMVVGKRFL--SATATDEKAEKCVKAVDEFMRLAGVFTVGDAIPYLRWLDFGG 254

Query: 256 QVKVMKNIATRFDMVLSEWLEEHKR----------------------------------- 315
             K MK  A   D+++SEWLEEH++                                   
Sbjct: 255 YEKAMKETAKELDVMISEWLEEHRQKRALGEGVDGAQDFMNVMLSSLDGKTIDGIDADTL 314

Query: 316 ---------------------------------------------RERRVKESDIGKLVY 375
                                                        ++R + ESDI  LVY
Sbjct: 315 IKSTVLTIIQAGTEASISTIIWAMCLILKNPLILENKAELDIQVGKDRCICESDISNLVY 374

Query: 376 LQSIIKETMRMYPAGPLLGPREFYKDCVVAGYFVPKGTQLIPNIWKIQTDSRVWPDPFEF 435
           LQ+++KET+R+Y  GPL  PREF +DC + GY V KGT+LI NIWKI TD  VW DPFEF
Sbjct: 375 LQAVVKETLRLYAPGPLSSPREFAEDCTLGGYHVKKGTRLITNIWKIHTDPNVWSDPFEF 434

Query: 436 KPERFLTSHKDVDLKGNNFELIPFGSGRRGCPGIAFGLQMVHFTLAGFLHSFDVKKPTKE 438
           KP+RFLT+HKD+D+KG++F+L+PFGSGRR CPGI+FGLQ VH  LA FLHSF++  P+ E
Sbjct: 435 KPDRFLTTHKDIDVKGHHFQLLPFGSGRRVCPGISFGLQTVHLALASFLHSFEILNPSTE 494

[Close]

BLAST of Lsi04G016100 vs. ExPASy Swiss-Prot
Match: Q43068 (Cytochrome P450 82A1 (Fragment) OS=Pisum sativum OX=3888 GN=CYP82A1 PE=2 SV=2)

HSP 1 Score: 390.2 bits (1001), Expect = 3.2e-107
Identity = 202/531 (38.04%), Postives = 283/531 (53.30%), Query Frame = 0


Query: 9   NPISITMLVVLFFISCYYLWRKWAQHKVTEQKQAPAPPGAWPVIGHLHLLRNAKLPHHAL 68
           N ++ T +  +  IS  +   +++  KV+  K+ P   G+WP++GHL L+RN + PH  L
Sbjct: 4   NYLNTTTIAFISLISLLFFLFRFS--KVSHTKEPPIISGSWPLLGHLPLMRNTQTPHKTL 63

Query: 69  GAMADKYGPIFRLQLGSRSALVVSSWEMAKESMCVNDAAAASRPGVSGTKYFSYDFAAFG 128
           GA+ DKYGPIF ++LG+ +ALV+S+WE+AKE    ND   +SRP     +  SY+ A  G
Sbjct: 64  GALVDKYGPIFTIKLGATNALVLSNWELAKECFTKNDIVVSSRPKPVAVELMSYNQAFIG 123

Query: 129 LAPYSPYWREIRKVTHMELLSNPRVDQVKSIMFGEINTSLTELYETW------------- 188
            APY  YWR++RK+  +E+LSN R++ +  I   E+ TS+ EL   W             
Sbjct: 124 WAPYGAYWRQLRKIVTLEILSNRRIELLSHIRVSEVQTSIKELVNVWSNQISSQYGLLDD 183

Query: 189 ------EAEKEESGQVQVEMKRWFGNVIVNMLLKIIIGKRCVGPNAEGGEKQAKDFQLAI 248
                   E   +  V VE+K+WF  + +NM+L++++GKRC G      +++AK F   I
Sbjct: 184 TKSSSTNDEPSTTDYVSVELKKWFAQLTLNMVLRMVVGKRCFGDVDVENKEEAKRFLENI 243

Query: 249 RDSFHLMGQGLLRDYIPIIGRLGFNGQVKVMKNIATRFDMVLSEWLEEHKR--------- 308
           RD   L+G   + D +P +  L   G  K MK  A +FD++L+EWLEEH+          
Sbjct: 244 RDFMRLIGTFTVGDGVPFLKWLDLGGHEKEMKKCAKKFDVMLNEWLEEHREKKGLGSEDK 303

Query: 309 ------------------------------------------------------------ 368
                                                                       
Sbjct: 304 VVGERDFMDAMLLVLKDKPIEGFDVDTIIKATTLELILGGSDTTAGTLTWAMCLLLKHPH 363

Query: 369 --------------RERRVKESDIGKLVYLQSIIKETMRMYPAGPLLGPREFYKDCVVAG 428
                         +ER V ESDI KLVYL +IIKET+R+YP  P   PREF +DC + G
Sbjct: 364 VLEKLKEELNTYIGKERCVNESDINKLVYLHAIIKETLRLYPPAPFSSPREFTEDCTIGG 423

Query: 429 YFVPKGTQLIPNIWKIQTDSRVWPDPFEFKPERFLTSHKDVDLKGNNFELIPFGSGRRGC 438
           Y + KGT+L+PN+WKI  D  VWPDP EFKPERFL++HKDVD++G NFEL+PFGSGRR C
Sbjct: 424 YHIKKGTRLMPNLWKIHRDPSVWPDPLEFKPERFLSTHKDVDVRGQNFELLPFGSGRRMC 483

[Close]

BLAST of Lsi04G016100 vs. ExPASy Swiss-Prot
Match: Q9SZ46 (Xanthotoxin 5-hydroxylase CYP82C4 OS=Arabidopsis thaliana OX=3702 GN=CYP82C4 PE=1 SV=1)

HSP 1 Score: 370.9 bits (951), Expect = 2.0e-101
Identity = 204/529 (38.56%), Postives = 281/529 (53.12%), Query Frame = 0


Query: 5   FPSFNPISITMLVVLFFISCYYLWRKWAQHKVTEQKQAPAPPGAWPVIGHLHLL-RNAKL 64
           F  F PI + + + LF            + K  +  +APAP GAWP+IGHLHLL    +L
Sbjct: 6   FSLFVPILVFVFIALF-----------KKSKKPKYVKAPAPSGAWPIIGHLHLLGGKEQL 65

Query: 65  PHHALGAMADKYGPIFRLQLGSRSALVVSSWEMAKESMCVNDAAAASRPGVSGTKYFSYD 124
            +  LG MAD YGP   LQLGS  A VVSS+E+AK+   VND A ASRP  +  K+  Y+
Sbjct: 66  LYRTLGKMADHYGPAMSLQLGSNEAFVVSSFEVAKDCFTVNDKALASRPMTAAAKHMGYN 125

Query: 125 FAAFGLAPYSPYWREIRKVTHMELLSNPRVDQVKSIMFGEINTSLTELYETWEAEKEESG 184
           FA FG APYS +WRE+RK+  +ELLSN R+  +K +   EI   + +LY  W  +   + 
Sbjct: 126 FAVFGFAPYSAFWREMRKIATIELLSNRRLQMLKHVRVSEITMGVKDLYSLW-FKNGGTK 185

Query: 185 QVQVEMKRWFGNVIVNMLLKIIIGKRCVGPN---AEGGEKQAKDFQLAIRDSFHLMGQGL 244
            V V++K W  ++ +NM+++++ GKR  G     +    ++A   + AI   FHL+G   
Sbjct: 186 PVMVDLKSWLEDMTLNMIVRMVAGKRYFGGGGSVSSEDTEEAMQCKKAIAKFFHLIGIFT 245

Query: 245 LRDYIPIIGRLGFNGQVKVMKNIATRFDMVLSEWLEEHKR-------------------- 304
           + D  P +      G  K MK   +  D++L  W+E H++                    
Sbjct: 246 VSDAFPTLSFFDLQGHEKEMKQTGSELDVILERWIENHRQQRKFSGTKENDSDFIDVMMS 305

Query: 305 ------------------------------------------------------------ 364
                                                                       
Sbjct: 306 LAEQGKLSHLQYDANTSIKSTCLALILGGSDTSASTLTWAISLLLNNKEMLKKAQDEIDI 365

Query: 365 ---RERRVKESDIGKLVYLQSIIKETMRMYPAGPLLGPREFYKDCVVAGYFVPKGTQLIP 424
              R+R V++SDI  LVYLQ+IIKET+R+YPAGPLLGPRE  +DC VAGY+VP GT+LI 
Sbjct: 366 HVGRDRNVEDSDIENLVYLQAIIKETLRLYPAGPLLGPREAMEDCTVAGYYVPCGTRLIV 425

Query: 425 NIWKIQTDSRVWPDPFEFKPERFLTSH-KDVDLKGNNFELIPFGSGRRGCPGIAFGLQMV 446
           N+WKIQ D +V+ +P EF+PERF+T   K+ D++G NFEL+PFGSGRR CPG +  +Q++
Sbjct: 426 NVWKIQRDPKVYMEPNEFRPERFITGEAKEFDVRGQNFELMPFGSGRRSCPGSSLAMQVL 485

[Close]

BLAST of Lsi04G016100 vs. ExPASy Swiss-Prot
Match: O81972 (Cytochrome P450 82A2 OS=Glycine max OX=3847 GN=CYP82A2 PE=2 SV=1)

HSP 1 Score: 362.5 bits (929), Expect = 7.1e-99
Identity = 190/503 (37.77%), Postives = 267/503 (53.08%), Query Frame = 0


Query: 18  VLFFISCYYLWRKWAQHKVTEQKQAPAPPGAWPVIGHLHLLRNAKLPHHALGAMADKYGP 77
           V+  I   YL+ +    K  E+   P   GAWP+IGHL LL  +K PH  LG +ADKYGP
Sbjct: 14  VVSLILLLYLFLRGGSWKSGEE-GPPTVAGAWPIIGHLPLLLGSKTPHKTLGDLADKYGP 73

Query: 78  IFRLQLGSRSALVVSSWEMAKESMCVNDAAAASRPGVSGTKYFSYDFAAFGLAPYSPYWR 137
           IF +++G+++A+VVS+WEMAKE    ND A +S P +       Y+ +   +APY PYWR
Sbjct: 74  IFSIKIGAKNAVVVSNWEMAKECYTTNDIAVSSLPDLISANLLCYNRSMIVVAPYGPYWR 133

Query: 138 EIRKVTHMELLSNPRVDQVKSIMFGEINTSLTELYETWEAEKE-ESGQVQVEMKRWFGNV 197
           ++RK+   E LS  RV+Q+  +   E+ +S+TEL+  W + K  +SG   VE+K+WF  +
Sbjct: 134 QLRKILMSEFLSPSRVEQLHHVRVSEVQSSITELFRDWRSNKNVQSGFATVELKQWFSLL 193

Query: 198 IVNMLLKIIIGKRCVGPNAEGGEKQAKDFQLAIRDSFHLMGQGLLRDYIPIIGRLGFNGQ 257
           + NM+L+++ GKR    +    EK A     A+ +   L     + D IP +    F G 
Sbjct: 194 VFNMILRMVCGKRYFSASTSDDEK-ANRCVKAVDEFVRLAATFTVGDAIPYLRWFDFGGY 253

Query: 258 VKVMKNIATRFDMVLSEWLEEHKR------------------------------------ 317
              M+      D ++ EWL+EH++                                    
Sbjct: 254 ENDMRETGKELDEIIGEWLDEHRQKRKMGENVQDLMSVLLSLLEGKTIEGMNVDIVIKSF 313

Query: 318 ------------------------------------------RERRVKESDIGKLVYLQS 377
                                                     +ER + ESD+ KL YLQ+
Sbjct: 314 VLTVIQAGTEASITTLIWATSLILNNPSVLEKLKAELDIQVGKERYICESDLSKLTYLQA 373

Query: 378 IIKETMRMYPAGPLLGPREFYKDCVVAGYFVPKGTQLIPNIWKIQTDSRVWPDPFEFKPE 437
           ++KET+R+YP  PL  PREF +DC + GY V KGT+LI N+ KI TD  VW +P EFKPE
Sbjct: 374 VVKETLRLYPPAPLSRPREFEEDCTIGGYTVKKGTRLITNLSKIHTDHNVWSNPLEFKPE 433

Query: 438 RFLTSHKDVDLKGNNFELIPFGSGRRGCPGIAFGLQMVHFTLAGFLHSFDVKKPTKEPID 442
           RFLT+ KD+D+KG +F+L+PFG GRR CPGI  GLQ V  TLA FLHSF++  P+ EP+D
Sbjct: 434 RFLTTDKDIDMKGQHFQLLPFGGGRRICPGINLGLQTVRLTLASFLHSFEILNPSTEPLD 493

[Close]

BLAST of Lsi04G016100 vs. ExPASy TrEMBL
Match: A0A0A0LD43 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G852590 PE=3 SV=1)

HSP 1 Score: 812.4 bits (2097), Expect = 9.6e-232
Identity = 399/531 (75.14%), Postives = 427/531 (80.41%), Query Frame = 0


Query: 1   MEFQFPSFNPISITMLVVLFFISCYYLWRKWAQHKVTEQKQAPAPPGAWPVIGHLHLLRN 60
           +EFQFP FNP SITMLV LFFISCYYLW+KW Q+K TE KQAPAPPGAWP+IGHLH+L N
Sbjct: 2   VEFQFPFFNPKSITMLVFLFFISCYYLWQKWQQYKSTEPKQAPAPPGAWPIIGHLHMLHN 61

Query: 61  AKLPHHALGAMADKYGPIFRLQLGSRSALVVSSWEMAKESMCVNDAAAASRPGVSGTKYF 120
            KLPHHALGAMADKYGP+FRLQLGSRSALVVSSWEMAKESMCVNDAAAASRPGVSGTK+F
Sbjct: 62  VKLPHHALGAMADKYGPLFRLQLGSRSALVVSSWEMAKESMCVNDAAAASRPGVSGTKHF 121

Query: 121 SYDFAAFGLAPYSPYWREIRKVTHMELLSNPRVDQVKSIMFGEINTSLTELYETWEAEKE 180
           SYDFAAFGLAPYSPYWREIRKVTHMELLSNPRVDQ K+ MFGE+ TSL EL+ETW A+K+
Sbjct: 122 SYDFAAFGLAPYSPYWREIRKVTHMELLSNPRVDQFKNTMFGEVKTSLRELHETWAAQKD 181

Query: 181 ESGQVQVEMKRWFGNVIVNMLLKIIIGKRCVGPNAEGGEKQAKDFQLAIRDSFHLMGQGL 240
            SGQV+VEMKRWFG+VIVNMLLKIIIGKRCVGPNAEGGEKQAKDFQLAIRDSFHLMGQGL
Sbjct: 182 GSGQVEVEMKRWFGDVIVNMLLKIIIGKRCVGPNAEGGEKQAKDFQLAIRDSFHLMGQGL 241

Query: 241 LRDYIPIIGRLGFNGQVKVMKNIATRFDMVLSEWLEEHK--------------------- 300
           LRDYIP+IGRLGFNGQVKVM+NIATRFDMVL EWL+EHK                     
Sbjct: 242 LRDYIPLIGRLGFNGQVKVMENIATRFDMVLREWLDEHKLNRTSSCCGRKDGDFMDALVS 301

Query: 301 ------------------------------------------------------------ 360
                                                                       
Sbjct: 302 LYDGKEIEGYYDGDTIIKATTLNMVAGGTESTTVTLTWAMSLLINNPHVLERAQQELDTV 361

Query: 361 -RRERRVKESDIGKLVYLQSIIKETMRMYPAGPLLGPREFYKDCVVAGYFVPKGTQLIPN 420
             R+R++KESDI  LVYL+SIIKETMRMYPAGPLLGPREFYKDC+VAGYFVPKGTQLIPN
Sbjct: 362 VGRDRQLKESDIPNLVYLKSIIKETMRMYPAGPLLGPREFYKDCIVAGYFVPKGTQLIPN 421

Query: 421 IWKIQTDSRVWPDPFEFKPERFLTSHKDVDLKGNNFELIPFGSGRRGCPGIAFGLQMVHF 450
           IWKIQTD RVWPDPFEFKPERFLT+HK+VDLKGNNFELIPFGSGRRGCPG+AFGLQMVHF
Sbjct: 422 IWKIQTDPRVWPDPFEFKPERFLTTHKNVDLKGNNFELIPFGSGRRGCPGLAFGLQMVHF 481

[Close]

BLAST of Lsi04G016100 vs. ExPASy TrEMBL
Match: A0A1S3CMR1 (cytochrome P450 CYP82D47-like OS=Cucumis melo OX=3656 GN=LOC103502568 PE=3 SV=1)

HSP 1 Score: 798.9 bits (2062), Expect = 1.1e-227
Identity = 393/531 (74.01%), Postives = 423/531 (79.66%), Query Frame = 0


Query: 1   MEFQFPSFNPISITMLVVLFFISCYYLWRKWAQHKVTEQKQAPAPPGAWPVIGHLHLLRN 60
           +EFQFP FNP SITML++ FFISCYYLWRKW Q+K T  KQAPAPPGA P+IGHLH+L N
Sbjct: 2   IEFQFPFFNPKSITMLILWFFISCYYLWRKWKQYKSTNLKQAPAPPGALPIIGHLHMLHN 61

Query: 61  AKLPHHALGAMADKYGPIFRLQLGSRSALVVSSWEMAKESMCVNDAAAASRPGVSGTKYF 120
            KLPHHALGAMADKYGPIFRLQLGSRSALVVSSWEMAKESMCVNDAAAASRP VSGTK+F
Sbjct: 62  VKLPHHALGAMADKYGPIFRLQLGSRSALVVSSWEMAKESMCVNDAAAASRPAVSGTKHF 121

Query: 121 SYDFAAFGLAPYSPYWREIRKVTHMELLSNPRVDQVKSIMFGEINTSLTELYETWEAEKE 180
           SYDFAAFGLAPY PYWREIRKVTHMELLSNPRVDQ K+ MFGE+ TSLTEL+ETW A+K+
Sbjct: 122 SYDFAAFGLAPYGPYWREIRKVTHMELLSNPRVDQFKNTMFGEVKTSLTELHETWAAQKD 181

Query: 181 ESGQVQVEMKRWFGNVIVNMLLKIIIGKRCVGPNAEGGEKQAKDFQLAIRDSFHLMGQGL 240
            SGQV+VEMKRWFG+VIVNMLLKIIIGKRCVGPNA+GGEKQAKDFQLAIRDSFHLMGQGL
Sbjct: 182 GSGQVEVEMKRWFGDVIVNMLLKIIIGKRCVGPNAKGGEKQAKDFQLAIRDSFHLMGQGL 241

Query: 241 LRDYIPIIGRLGFNGQVKVMKNIATRFDMVLSEWLEEHKR-------------------- 300
           LRDY+P+IG LGFNGQVKVM+NIATRFD+VLSEWL+EHKR                    
Sbjct: 242 LRDYVPLIGWLGFNGQVKVMENIATRFDVVLSEWLDEHKRNRTSSCCGRRDGDFMDALVS 301

Query: 301 ------------------------------------------------------------ 360
                                                                       
Sbjct: 302 LYDGKGIEGYYDGDTIIKATTLNMVAGGTESTTVTLTWAMSLLLNNPHVLERAQQELDTV 361

Query: 361 --RERRVKESDIGKLVYLQSIIKETMRMYPAGPLLGPREFYKDCVVAGYFVPKGTQLIPN 420
             R+RR+KESDI  LVYLQSIIKETMRMYPAGPLLGPREFYKDC+VAGYFVPKGTQLIPN
Sbjct: 362 VGRDRRLKESDIPNLVYLQSIIKETMRMYPAGPLLGPREFYKDCIVAGYFVPKGTQLIPN 421

Query: 421 IWKIQTDSRVWPDPFEFKPERFLTSHKDVDLKGNNFELIPFGSGRRGCPGIAFGLQMVHF 450
           +W+IQTD RVWPDPF+FKPERFLT+HK+VDLKGNNFELIPFGSGRRGCPG AFGLQMVHF
Sbjct: 422 LWRIQTDPRVWPDPFKFKPERFLTTHKNVDLKGNNFELIPFGSGRRGCPGAAFGLQMVHF 481

[Close]

BLAST of Lsi04G016100 vs. ExPASy TrEMBL
Match: A0A6J1FBI1 (cytochrome P450 82A3-like OS=Cucurbita moschata OX=3662 GN=LOC111442563 PE=3 SV=1)

HSP 1 Score: 787.3 bits (2032), Expect = 3.3e-224
Identity = 393/531 (74.01%), Postives = 421/531 (79.28%), Query Frame = 0


Query: 1   MEFQFPSFNPISITMLVVLFFI-SCYYLWRKWAQHKVTEQKQAPAPPGAWPVIGHLHLLR 60
           M+FQ PS    SIT+L++LFFI SCYYLWRKW QHKV E+KQAP+PPGAWP+IGHLHLL 
Sbjct: 1   MDFQLPS----SITVLLLLFFITSCYYLWRKWQQHKVAERKQAPSPPGAWPIIGHLHLLH 60

Query: 61  NAKLPHHALGAMADKYGPIFRLQLGSRSALVVSSWEMAKESMCVNDAAAASRPGVSGTKY 120
           NAKLPHHALGAMADKYGPIFRLQLGSRSALVVSSWEMAKESMC NDAAAASRPGVSGTKY
Sbjct: 61  NAKLPHHALGAMADKYGPIFRLQLGSRSALVVSSWEMAKESMCFNDAAAASRPGVSGTKY 120

Query: 121 FSYDFAAFGLAPYSPYWREIRKVTHMELLSNPRVDQVKSIMFGEINTSLTELYETWEAEK 180
           FSYDFAAFGLAPYSPYWREIRKVTH+ELLSNPRV QVKS M  +INTSLTEL+ETW A+K
Sbjct: 121 FSYDFAAFGLAPYSPYWREIRKVTHIELLSNPRVHQVKSTMLAQINTSLTELHETWGAQK 180

Query: 181 EESGQVQVEMKRWFGNVIVNMLLKIIIGKRCVGPNAEGGEKQAKDFQLAIRDSFHLMGQG 240
           +ESGQV VEMKRWFG+VIVNMLLKIIIGKRCVGPNAEGGEK+AKD QLAIRDSFHLMGQG
Sbjct: 181 DESGQVAVEMKRWFGDVIVNMLLKIIIGKRCVGPNAEGGEKEAKDIQLAIRDSFHLMGQG 240

Query: 241 LLRDYIPIIGRLGFNGQVKVMKNIATRFDMVLSEWLEEHKR------------------- 300
           LL+DYIP+IGRLGFNGQV VMKNIA RFDMVLSEWLEEHKR                   
Sbjct: 241 LLQDYIPLIGRLGFNGQVPVMKNIAKRFDMVLSEWLEEHKRRRSASSGENDRDFMDALIS 300

Query: 301 ------------------------------------------------------------ 360
                                                                       
Sbjct: 301 LYNGKEIEGYYDGDTITKATTLNMFAGGTESTTVTLTWAMSLLLNNPHVLEKAWQELDAV 360

Query: 361 --RERRVKESDIGKLVYLQSIIKETMRMYPAGPLLGPREFYKDCVVAGYFVPKGTQLIPN 420
             RERRV E D+GKLVYLQ+IIKETMR+YPAGPLLGPREF+KDCVV GYFVPKGTQLIPN
Sbjct: 361 VGRERRVTEDDVGKLVYLQAIIKETMRLYPAGPLLGPREFHKDCVVGGYFVPKGTQLIPN 420

Query: 421 IWKIQTDSRVWPDPFEFKPERFLTSHKDVDLKGNNFELIPFGSGRRGCPGIAFGLQMVHF 450
           IWKIQTD RVW +PFEFKPERFLT+HKDVDLKGN+FELIPFGSGRRGCPG+AFGLQM+HF
Sbjct: 421 IWKIQTDPRVWCEPFEFKPERFLTTHKDVDLKGNHFELIPFGSGRRGCPGVAFGLQMMHF 480

[Close]

BLAST of Lsi04G016100 vs. ExPASy TrEMBL
Match: A0A6J1IGS1 (cytochrome P450 82A3-like OS=Cucurbita maxima OX=3661 GN=LOC111476809 PE=3 SV=1)

HSP 1 Score: 773.5 bits (1996), Expect = 4.9e-220
Identity = 389/531 (73.26%), Postives = 416/531 (78.34%), Query Frame = 0


Query: 1   MEFQFPSFNPISITMLVVLFFI-SCYYLWRKWAQHKVTEQKQAPAPPGAWPVIGHLHLLR 60
           MEFQ PS    SI +L+ LFFI SCYYLWRKW QHK+ ++KQAP+PPGAWP+IGHLHLL 
Sbjct: 1   MEFQLPS----SIIVLLHLFFITSCYYLWRKWQQHKLADRKQAPSPPGAWPIIGHLHLLH 60

Query: 61  NAKLPHHALGAMADKYGPIFRLQLGSRSALVVSSWEMAKESMCVNDAAAASRPGVSGTKY 120
           NAKLPHHALGAMADKYGPIF LQLGSRSALVVSSWEMAKESMCVNDAAAASRPGVSGTKY
Sbjct: 61  NAKLPHHALGAMADKYGPIFSLQLGSRSALVVSSWEMAKESMCVNDAAAASRPGVSGTKY 120

Query: 121 FSYDFAAFGLAPYSPYWREIRKVTHMELLSNPRVDQVKSIMFGEINTSLTELYETWEAEK 180
           FSYDFAAFGLAPYSPYWREIRKVTHMELLSNPRV +V S M  EINTSLTEL+ETW A+K
Sbjct: 121 FSYDFAAFGLAPYSPYWREIRKVTHMELLSNPRVYKVASTMLAEINTSLTELHETWGAQK 180

Query: 181 EESGQVQVEMKRWFGNVIVNMLLKIIIGKRCVGPNAEGGEKQAKDFQLAIRDSFHLMGQG 240
           +ESGQV VEMKRWFG+VIVNMLLKIIIGKRCVGPNAEGGEK+AKD QLAIRDSFHLMGQG
Sbjct: 181 DESGQVAVEMKRWFGDVIVNMLLKIIIGKRCVGPNAEGGEKEAKDIQLAIRDSFHLMGQG 240

Query: 241 LLRDYIPIIGRLGFNGQVKVMKNIATRFDMVLSEWLEEHKR------------------- 300
           LL+DYIP+IGRLGFNGQV VM+NIA RFDMVLS+WLEEHKR                   
Sbjct: 241 LLQDYIPLIGRLGFNGQVPVMENIAKRFDMVLSQWLEEHKRRRSASSGEKDRDFMDALIS 300

Query: 301 ------------------------------------------------------------ 360
                                                                       
Sbjct: 301 LHNGKGIEGYYDGDTITKATTLNMIAGGTESTTVTLTWAMSLLLNNPHVLEKAWQELDAV 360

Query: 361 --RERRVKESDIGKLVYLQSIIKETMRMYPAGPLLGPREFYKDCVVAGYFVPKGTQLIPN 420
             RERRV E DI KLVYLQ+IIKETMR+YPAGPLLGPREF+KDCVV GYFVPKGTQLIPN
Sbjct: 361 VGRERRVTEDDISKLVYLQAIIKETMRLYPAGPLLGPREFHKDCVVGGYFVPKGTQLIPN 420

Query: 421 IWKIQTDSRVWPDPFEFKPERFLTSHKDVDLKGNNFELIPFGSGRRGCPGIAFGLQMVHF 450
           IWKIQTD RVW +PFEFKPERFLT+HKDVDLKGN+FELIPFGSGRRGCP  AFGLQM+HF
Sbjct: 421 IWKIQTDPRVWCEPFEFKPERFLTTHKDVDLKGNHFELIPFGSGRRGCPAAAFGLQMMHF 480

[Close]

BLAST of Lsi04G016100 vs. ExPASy TrEMBL
Match: A0A6J1DJE5 (cytochrome P450 82A3-like OS=Momordica charantia OX=3673 GN=LOC111021426 PE=3 SV=1)

HSP 1 Score: 707.6 bits (1825), Expect = 3.3e-200
Identity = 354/531 (66.67%), Postives = 393/531 (74.01%), Query Frame = 0


Query: 1   MEFQFPSFNPISITMLVVLFFISCYYLWRKWAQHKVTEQKQAPAPPGAWPVIGHLHLLRN 60
           ME QFP FNP S+  LV +F    YYL RKW      E+KQAP+PPGAWP+IGHLHLL N
Sbjct: 49  MESQFPPFNPTSVATLVGIFVFCYYYLRRKW-----QERKQAPSPPGAWPIIGHLHLLHN 108

Query: 61  AKLPHHALGAMADKYGPIFRLQLGSRSALVVSSWEMAKESMCVNDAAAASRPGVSGTKYF 120
           AKLPHH LGAMADKYGPIFRLQLG RSALVVS+WEMAKESMC+NDAAA SRPG+SGTK+F
Sbjct: 109 AKLPHHTLGAMADKYGPIFRLQLGCRSALVVSNWEMAKESMCINDAAAVSRPGISGTKHF 168

Query: 121 SYDFAAFGLAPYSPYWREIRKVTHMELLSNPRVDQVKSIMFGEINTSLTELYETWEAEKE 180
           SYD+AAFGLAPYSPYWRE+RK+TH+ELLSN RVDQVK++MF +I+ SL EL+        
Sbjct: 169 SYDYAAFGLAPYSPYWREMRKITHLELLSNSRVDQVKNLMFAQIDMSLAELH-------A 228

Query: 181 ESGQVQVEMKRWFGNVIVNMLLKIIIGKRCVGPNAEGGEKQAKDFQLAIRDSFHLMGQGL 240
            S Q+ VEMKRWFG+VIVNMLLKIIIGKRCVGPNAEGGEK AKD Q+AIRDSFHLMGQGL
Sbjct: 229 GSAQIPVEMKRWFGDVIVNMLLKIIIGKRCVGPNAEGGEKGAKDLQMAIRDSFHLMGQGL 288

Query: 241 LRDYIPIIGRLGFNGQVKVMKNIATRFDMVLSEWLEEHKR-------------------- 300
           L DYIP+IGRLGF+GQVKVM++IA RFD+++SEWLEEHKR                    
Sbjct: 289 LTDYIPLIGRLGFDGQVKVMESIAKRFDVIMSEWLEEHKRKRDYGLGQNQDGDFMDSLIS 348

Query: 301 ------------------------------------------------------------ 360
                                                                       
Sbjct: 349 LLDGKGIQGYYDADTIIKATTLNMIAGGTESTTVTLTWTMTLLLNNPHTLEMAQQELDAV 408

Query: 361 --RERRVKESDIGKLVYLQSIIKETMRMYPAGPLLGPREFYKDCVVAGYFVPKGTQLIPN 420
             R+RRV ESDI KL YLQ+++KE MRMYPAGPLLGPREF  DCVVAGYFVP+GTQLIPN
Sbjct: 409 VGRDRRVNESDISKLHYLQAVVKEAMRMYPAGPLLGPREFSSDCVVAGYFVPRGTQLIPN 468

Query: 421 IWKIQTDSRVWPDPFEFKPERFLTSHKDVDLKGNNFELIPFGSGRRGCPGIAFGLQMVHF 450
           IWKIQTD RVWP+PFEFKPERFLT+HKDVD KGN+FELIPFGSGRRGCPG+AFGLQMVHF
Sbjct: 469 IWKIQTDPRVWPNPFEFKPERFLTTHKDVDAKGNHFELIPFGSGRRGCPGVAFGLQMVHF 528

[Close]

BLAST of Lsi04G016100 vs. NCBI nr
Match: XP_004147824.1 (cytochrome P450 CYP82D47 [Cucumis sativus] >KGN59910.1 hypothetical protein Csa_000849 [Cucumis sativus])

HSP 1 Score: 812.4 bits (2097), Expect = 2.0e-231
Identity = 399/531 (75.14%), Postives = 427/531 (80.41%), Query Frame = 0


Query: 1   MEFQFPSFNPISITMLVVLFFISCYYLWRKWAQHKVTEQKQAPAPPGAWPVIGHLHLLRN 60
           +EFQFP FNP SITMLV LFFISCYYLW+KW Q+K TE KQAPAPPGAWP+IGHLH+L N
Sbjct: 2   VEFQFPFFNPKSITMLVFLFFISCYYLWQKWQQYKSTEPKQAPAPPGAWPIIGHLHMLHN 61

Query: 61  AKLPHHALGAMADKYGPIFRLQLGSRSALVVSSWEMAKESMCVNDAAAASRPGVSGTKYF 120
            KLPHHALGAMADKYGP+FRLQLGSRSALVVSSWEMAKESMCVNDAAAASRPGVSGTK+F
Sbjct: 62  VKLPHHALGAMADKYGPLFRLQLGSRSALVVSSWEMAKESMCVNDAAAASRPGVSGTKHF 121

Query: 121 SYDFAAFGLAPYSPYWREIRKVTHMELLSNPRVDQVKSIMFGEINTSLTELYETWEAEKE 180
           SYDFAAFGLAPYSPYWREIRKVTHMELLSNPRVDQ K+ MFGE+ TSL EL+ETW A+K+
Sbjct: 122 SYDFAAFGLAPYSPYWREIRKVTHMELLSNPRVDQFKNTMFGEVKTSLRELHETWAAQKD 181

Query: 181 ESGQVQVEMKRWFGNVIVNMLLKIIIGKRCVGPNAEGGEKQAKDFQLAIRDSFHLMGQGL 240
            SGQV+VEMKRWFG+VIVNMLLKIIIGKRCVGPNAEGGEKQAKDFQLAIRDSFHLMGQGL
Sbjct: 182 GSGQVEVEMKRWFGDVIVNMLLKIIIGKRCVGPNAEGGEKQAKDFQLAIRDSFHLMGQGL 241

Query: 241 LRDYIPIIGRLGFNGQVKVMKNIATRFDMVLSEWLEEHK--------------------- 300
           LRDYIP+IGRLGFNGQVKVM+NIATRFDMVL EWL+EHK                     
Sbjct: 242 LRDYIPLIGRLGFNGQVKVMENIATRFDMVLREWLDEHKLNRTSSCCGRKDGDFMDALVS 301

Query: 301 ------------------------------------------------------------ 360
                                                                       
Sbjct: 302 LYDGKEIEGYYDGDTIIKATTLNMVAGGTESTTVTLTWAMSLLINNPHVLERAQQELDTV 361

Query: 361 -RRERRVKESDIGKLVYLQSIIKETMRMYPAGPLLGPREFYKDCVVAGYFVPKGTQLIPN 420
             R+R++KESDI  LVYL+SIIKETMRMYPAGPLLGPREFYKDC+VAGYFVPKGTQLIPN
Sbjct: 362 VGRDRQLKESDIPNLVYLKSIIKETMRMYPAGPLLGPREFYKDCIVAGYFVPKGTQLIPN 421

Query: 421 IWKIQTDSRVWPDPFEFKPERFLTSHKDVDLKGNNFELIPFGSGRRGCPGIAFGLQMVHF 450
           IWKIQTD RVWPDPFEFKPERFLT+HK+VDLKGNNFELIPFGSGRRGCPG+AFGLQMVHF
Sbjct: 422 IWKIQTDPRVWPDPFEFKPERFLTTHKNVDLKGNNFELIPFGSGRRGCPGLAFGLQMVHF 481

[Close]

BLAST of Lsi04G016100 vs. NCBI nr
Match: XP_038897047.1 (cytochrome P450 CYP82D47-like [Benincasa hispida])

HSP 1 Score: 805.8 bits (2080), Expect = 1.8e-229
Identity = 399/531 (75.14%), Postives = 426/531 (80.23%), Query Frame = 0


Query: 1   MEFQFPSFNPISITMLVVLFFISCYYLWRKWAQHKVTEQKQAPAPPGAWPVIGHLHLLRN 60
           MEFQFPSFNP SI ML VLFFISCYYLWRK  QH V+E+KQAP  PGAWP+IGHLHL+ N
Sbjct: 2   MEFQFPSFNPESIAMLFVLFFISCYYLWRKLEQHNVSERKQAPVLPGAWPIIGHLHLMHN 61

Query: 61  AKLPHHALGAMADKYGPIFRLQLGSRSALVVSSWEMAKESMCVNDAAAASRPGVSGTKYF 120
           AKLPHHALGAMADKYGPIFRLQLGSRSALVVSSWEMAKESMCVNDAAAASRPGVSGTK+F
Sbjct: 62  AKLPHHALGAMADKYGPIFRLQLGSRSALVVSSWEMAKESMCVNDAAAASRPGVSGTKHF 121

Query: 121 SYDFAAFGLAPYSPYWREIRKVTHMELLSNPRVDQVKSIMFGEINTSLTELYETWEAEKE 180
           SYDFAAFGLAPYSPYWREIRKVTHMELLSN RVDQ K+ MFGEI TSLTEL++TW A+K+
Sbjct: 122 SYDFAAFGLAPYSPYWREIRKVTHMELLSNQRVDQFKNTMFGEIKTSLTELHKTWAAQKD 181

Query: 181 ESGQVQVEMKRWFGNVIVNMLLKIIIGKRCVGPNAEGGEKQAKDFQLAIRDSFHLMGQGL 240
           ESGQVQVEMKRWFG+VI+NMLLKIIIGKRCVGPNAEGGEKQAKDFQLAIRDSFHLMGQGL
Sbjct: 182 ESGQVQVEMKRWFGDVILNMLLKIIIGKRCVGPNAEGGEKQAKDFQLAIRDSFHLMGQGL 241

Query: 241 LRDYIPIIGRLGFNGQVKVMKNIATRFDMVLSEWLEEHKR-------------------- 300
           LRDYIP+IGRLGFNGQVKVM+NIATRFDMVL EWLEEHKR                    
Sbjct: 242 LRDYIPLIGRLGFNGQVKVMENIATRFDMVLREWLEEHKRKRASSSSGKKNEDFMDALLS 301

Query: 301 ------------------------------------------------------------ 360
                                                                       
Sbjct: 302 LYDGKRIEGYYDGDTIIKATTLNMIAGGTESTTVTLTWAMSLLLNNPHVLEKAQQELDTV 361

Query: 361 --RERRVKESDIGKLVYLQSIIKETMRMYPAGPLLGPREFYKDCVVAGYFVPKGTQLIPN 420
             R+RR+K+SDI  LVYL  IIKETMR+YPAGPLLGPREFYKDC+V GYFVPKGTQLIPN
Sbjct: 362 VGRDRRLKDSDIPNLVYLHCIIKETMRLYPAGPLLGPREFYKDCIVDGYFVPKGTQLIPN 421

Query: 421 IWKIQTDSRVWPDPFEFKPERFLTSHKDVDLKGNNFELIPFGSGRRGCPGIAFGLQMVHF 450
           IWKIQTD RVWPDPFEFKPERFLT+HK+VDLKGNNFELIPFG+GRRGCPGI+FGLQMVHF
Sbjct: 422 IWKIQTDPRVWPDPFEFKPERFLTTHKNVDLKGNNFELIPFGTGRRGCPGISFGLQMVHF 481

[Close]

BLAST of Lsi04G016100 vs. NCBI nr
Match: XP_008464762.1 (PREDICTED: cytochrome P450 CYP82D47-like [Cucumis melo])

HSP 1 Score: 798.9 bits (2062), Expect = 2.3e-227
Identity = 393/531 (74.01%), Postives = 423/531 (79.66%), Query Frame = 0


Query: 1   MEFQFPSFNPISITMLVVLFFISCYYLWRKWAQHKVTEQKQAPAPPGAWPVIGHLHLLRN 60
           +EFQFP FNP SITML++ FFISCYYLWRKW Q+K T  KQAPAPPGA P+IGHLH+L N
Sbjct: 2   IEFQFPFFNPKSITMLILWFFISCYYLWRKWKQYKSTNLKQAPAPPGALPIIGHLHMLHN 61

Query: 61  AKLPHHALGAMADKYGPIFRLQLGSRSALVVSSWEMAKESMCVNDAAAASRPGVSGTKYF 120
            KLPHHALGAMADKYGPIFRLQLGSRSALVVSSWEMAKESMCVNDAAAASRP VSGTK+F
Sbjct: 62  VKLPHHALGAMADKYGPIFRLQLGSRSALVVSSWEMAKESMCVNDAAAASRPAVSGTKHF 121

Query: 121 SYDFAAFGLAPYSPYWREIRKVTHMELLSNPRVDQVKSIMFGEINTSLTELYETWEAEKE 180
           SYDFAAFGLAPY PYWREIRKVTHMELLSNPRVDQ K+ MFGE+ TSLTEL+ETW A+K+
Sbjct: 122 SYDFAAFGLAPYGPYWREIRKVTHMELLSNPRVDQFKNTMFGEVKTSLTELHETWAAQKD 181

Query: 181 ESGQVQVEMKRWFGNVIVNMLLKIIIGKRCVGPNAEGGEKQAKDFQLAIRDSFHLMGQGL 240
            SGQV+VEMKRWFG+VIVNMLLKIIIGKRCVGPNA+GGEKQAKDFQLAIRDSFHLMGQGL
Sbjct: 182 GSGQVEVEMKRWFGDVIVNMLLKIIIGKRCVGPNAKGGEKQAKDFQLAIRDSFHLMGQGL 241

Query: 241 LRDYIPIIGRLGFNGQVKVMKNIATRFDMVLSEWLEEHKR-------------------- 300
           LRDY+P+IG LGFNGQVKVM+NIATRFD+VLSEWL+EHKR                    
Sbjct: 242 LRDYVPLIGWLGFNGQVKVMENIATRFDVVLSEWLDEHKRNRTSSCCGRRDGDFMDALVS 301

Query: 301 ------------------------------------------------------------ 360
                                                                       
Sbjct: 302 LYDGKGIEGYYDGDTIIKATTLNMVAGGTESTTVTLTWAMSLLLNNPHVLERAQQELDTV 361

Query: 361 --RERRVKESDIGKLVYLQSIIKETMRMYPAGPLLGPREFYKDCVVAGYFVPKGTQLIPN 420
             R+RR+KESDI  LVYLQSIIKETMRMYPAGPLLGPREFYKDC+VAGYFVPKGTQLIPN
Sbjct: 362 VGRDRRLKESDIPNLVYLQSIIKETMRMYPAGPLLGPREFYKDCIVAGYFVPKGTQLIPN 421

Query: 421 IWKIQTDSRVWPDPFEFKPERFLTSHKDVDLKGNNFELIPFGSGRRGCPGIAFGLQMVHF 450
           +W+IQTD RVWPDPF+FKPERFLT+HK+VDLKGNNFELIPFGSGRRGCPG AFGLQMVHF
Sbjct: 422 LWRIQTDPRVWPDPFKFKPERFLTTHKNVDLKGNNFELIPFGSGRRGCPGAAFGLQMVHF 481

[Close]

BLAST of Lsi04G016100 vs. NCBI nr
Match: XP_022935740.1 (cytochrome P450 82A3-like [Cucurbita moschata])

HSP 1 Score: 787.3 bits (2032), Expect = 6.8e-224
Identity = 393/531 (74.01%), Postives = 421/531 (79.28%), Query Frame = 0


Query: 1   MEFQFPSFNPISITMLVVLFFI-SCYYLWRKWAQHKVTEQKQAPAPPGAWPVIGHLHLLR 60
           M+FQ PS    SIT+L++LFFI SCYYLWRKW QHKV E+KQAP+PPGAWP+IGHLHLL 
Sbjct: 1   MDFQLPS----SITVLLLLFFITSCYYLWRKWQQHKVAERKQAPSPPGAWPIIGHLHLLH 60

Query: 61  NAKLPHHALGAMADKYGPIFRLQLGSRSALVVSSWEMAKESMCVNDAAAASRPGVSGTKY 120
           NAKLPHHALGAMADKYGPIFRLQLGSRSALVVSSWEMAKESMC NDAAAASRPGVSGTKY
Sbjct: 61  NAKLPHHALGAMADKYGPIFRLQLGSRSALVVSSWEMAKESMCFNDAAAASRPGVSGTKY 120

Query: 121 FSYDFAAFGLAPYSPYWREIRKVTHMELLSNPRVDQVKSIMFGEINTSLTELYETWEAEK 180
           FSYDFAAFGLAPYSPYWREIRKVTH+ELLSNPRV QVKS M  +INTSLTEL+ETW A+K
Sbjct: 121 FSYDFAAFGLAPYSPYWREIRKVTHIELLSNPRVHQVKSTMLAQINTSLTELHETWGAQK 180

Query: 181 EESGQVQVEMKRWFGNVIVNMLLKIIIGKRCVGPNAEGGEKQAKDFQLAIRDSFHLMGQG 240
           +ESGQV VEMKRWFG+VIVNMLLKIIIGKRCVGPNAEGGEK+AKD QLAIRDSFHLMGQG
Sbjct: 181 DESGQVAVEMKRWFGDVIVNMLLKIIIGKRCVGPNAEGGEKEAKDIQLAIRDSFHLMGQG 240

Query: 241 LLRDYIPIIGRLGFNGQVKVMKNIATRFDMVLSEWLEEHKR------------------- 300
           LL+DYIP+IGRLGFNGQV VMKNIA RFDMVLSEWLEEHKR                   
Sbjct: 241 LLQDYIPLIGRLGFNGQVPVMKNIAKRFDMVLSEWLEEHKRRRSASSGENDRDFMDALIS 300

Query: 301 ------------------------------------------------------------ 360
                                                                       
Sbjct: 301 LYNGKEIEGYYDGDTITKATTLNMFAGGTESTTVTLTWAMSLLLNNPHVLEKAWQELDAV 360

Query: 361 --RERRVKESDIGKLVYLQSIIKETMRMYPAGPLLGPREFYKDCVVAGYFVPKGTQLIPN 420
             RERRV E D+GKLVYLQ+IIKETMR+YPAGPLLGPREF+KDCVV GYFVPKGTQLIPN
Sbjct: 361 VGRERRVTEDDVGKLVYLQAIIKETMRLYPAGPLLGPREFHKDCVVGGYFVPKGTQLIPN 420

Query: 421 IWKIQTDSRVWPDPFEFKPERFLTSHKDVDLKGNNFELIPFGSGRRGCPGIAFGLQMVHF 450
           IWKIQTD RVW +PFEFKPERFLT+HKDVDLKGN+FELIPFGSGRRGCPG+AFGLQM+HF
Sbjct: 421 IWKIQTDPRVWCEPFEFKPERFLTTHKDVDLKGNHFELIPFGSGRRGCPGVAFGLQMMHF 480

[Close]

BLAST of Lsi04G016100 vs. NCBI nr
Match: XP_023535455.1 (cytochrome P450 82A3-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 777.7 bits (2007), Expect = 5.4e-221
Identity = 389/531 (73.26%), Postives = 418/531 (78.72%), Query Frame = 0


Query: 1   MEFQFPSFNPISITMLVVLFFI-SCYYLWRKWAQHKVTEQKQAPAPPGAWPVIGHLHLLR 60
           M+FQ PS    SIT+L+ LFFI SCYYLWRKW  HKV E+KQAP+PPGAWP+IGHLHLL 
Sbjct: 1   MDFQLPS----SITVLLHLFFITSCYYLWRKWQHHKVAERKQAPSPPGAWPIIGHLHLLH 60

Query: 61  NAKLPHHALGAMADKYGPIFRLQLGSRSALVVSSWEMAKESMCVNDAAAASRPGVSGTKY 120
           NAKLPHHALGAMADKYGPIFRLQLGSRSALVVSSWEMAKESMCVNDAAAASRPGVSGTKY
Sbjct: 61  NAKLPHHALGAMADKYGPIFRLQLGSRSALVVSSWEMAKESMCVNDAAAASRPGVSGTKY 120

Query: 121 FSYDFAAFGLAPYSPYWREIRKVTHMELLSNPRVDQVKSIMFGEINTSLTELYETWEAEK 180
           FSYDFAAFGLAPYSPYWREIRKVTHMELLSNPRV QVKS M  +INTSLTEL+ETW A+K
Sbjct: 121 FSYDFAAFGLAPYSPYWREIRKVTHMELLSNPRVHQVKSPMLAQINTSLTELHETWGAQK 180

Query: 181 EESGQVQVEMKRWFGNVIVNMLLKIIIGKRCVGPNAEGGEKQAKDFQLAIRDSFHLMGQG 240
           +ESGQV VEMKRWFG+VI+NMLLKIIIGKRCVGPNAEGGEK+AKD QLAIRDSFHLMGQG
Sbjct: 181 DESGQVAVEMKRWFGDVIMNMLLKIIIGKRCVGPNAEGGEKEAKDIQLAIRDSFHLMGQG 240

Query: 241 LLRDYIPIIGRLGFNGQVKVMKNIATRFDMVLSEWLEEHKR------------------- 300
           LL+DYIP+IGRLGFNGQV VM+NIA RFDMVLS+WLEEHKR                   
Sbjct: 241 LLQDYIPLIGRLGFNGQVPVMENIAKRFDMVLSQWLEEHKRRRSASSGEKDRDFMDALIS 300

Query: 301 ------------------------------------------------------------ 360
                                                                       
Sbjct: 301 LYNGKEIEGYYDGDIITKATTLNMIAGGTESTTVTLTWAMSLLLNNPHVLEKAWQELDAV 360

Query: 361 --RERRVKESDIGKLVYLQSIIKETMRMYPAGPLLGPREFYKDCVVAGYFVPKGTQLIPN 420
             RERRV E DI KLVYLQ+IIKETMR+YPAGPLLGPREF+KDCVV GYFVPKGTQLIPN
Sbjct: 361 VGRERRVTEDDISKLVYLQAIIKETMRLYPAGPLLGPREFHKDCVVGGYFVPKGTQLIPN 420

Query: 421 IWKIQTDSRVWPDPFEFKPERFLTSHKDVDLKGNNFELIPFGSGRRGCPGIAFGLQMVHF 450
           IWKIQTD RVW +PFEFKPERF+ +HKDVDLKGN+FELIPFGSGRRGCPG+AFGLQM+HF
Sbjct: 421 IWKIQTDPRVWCEPFEFKPERFMRTHKDVDLKGNHFELIPFGSGRRGCPGVAFGLQMMHF 480

[Close]

BLAST of Lsi04G016100 vs. TAIR 10
Match: AT4G31940.1 (cytochrome P450, family 82, subfamily C, polypeptide 4 )

HSP 1 Score: 370.9 bits (951), Expect = 1.4e-102
Identity = 204/529 (38.56%), Postives = 281/529 (53.12%), Query Frame = 0


Query: 5   FPSFNPISITMLVVLFFISCYYLWRKWAQHKVTEQKQAPAPPGAWPVIGHLHLL-RNAKL 64
           F  F PI + + + LF            + K  +  +APAP GAWP+IGHLHLL    +L
Sbjct: 6   FSLFVPILVFVFIALF-----------KKSKKPKYVKAPAPSGAWPIIGHLHLLGGKEQL 65

Query: 65  PHHALGAMADKYGPIFRLQLGSRSALVVSSWEMAKESMCVNDAAAASRPGVSGTKYFSYD 124
            +  LG MAD YGP   LQLGS  A VVSS+E+AK+   VND A ASRP  +  K+  Y+
Sbjct: 66  LYRTLGKMADHYGPAMSLQLGSNEAFVVSSFEVAKDCFTVNDKALASRPMTAAAKHMGYN 125

Query: 125 FAAFGLAPYSPYWREIRKVTHMELLSNPRVDQVKSIMFGEINTSLTELYETWEAEKEESG 184
           FA FG APYS +WRE+RK+  +ELLSN R+  +K +   EI   + +LY  W  +   + 
Sbjct: 126 FAVFGFAPYSAFWREMRKIATIELLSNRRLQMLKHVRVSEITMGVKDLYSLW-FKNGGTK 185

Query: 185 QVQVEMKRWFGNVIVNMLLKIIIGKRCVGPN---AEGGEKQAKDFQLAIRDSFHLMGQGL 244
            V V++K W  ++ +NM+++++ GKR  G     +    ++A   + AI   FHL+G   
Sbjct: 186 PVMVDLKSWLEDMTLNMIVRMVAGKRYFGGGGSVSSEDTEEAMQCKKAIAKFFHLIGIFT 245

Query: 245 LRDYIPIIGRLGFNGQVKVMKNIATRFDMVLSEWLEEHKR-------------------- 304
           + D  P +      G  K MK   +  D++L  W+E H++                    
Sbjct: 246 VSDAFPTLSFFDLQGHEKEMKQTGSELDVILERWIENHRQQRKFSGTKENDSDFIDVMMS 305

Query: 305 ------------------------------------------------------------ 364
                                                                       
Sbjct: 306 LAEQGKLSHLQYDANTSIKSTCLALILGGSDTSASTLTWAISLLLNNKEMLKKAQDEIDI 365

Query: 365 ---RERRVKESDIGKLVYLQSIIKETMRMYPAGPLLGPREFYKDCVVAGYFVPKGTQLIP 424
              R+R V++SDI  LVYLQ+IIKET+R+YPAGPLLGPRE  +DC VAGY+VP GT+LI 
Sbjct: 366 HVGRDRNVEDSDIENLVYLQAIIKETLRLYPAGPLLGPREAMEDCTVAGYYVPCGTRLIV 425

Query: 425 NIWKIQTDSRVWPDPFEFKPERFLTSH-KDVDLKGNNFELIPFGSGRRGCPGIAFGLQMV 446
           N+WKIQ D +V+ +P EF+PERF+T   K+ D++G NFEL+PFGSGRR CPG +  +Q++
Sbjct: 426 NVWKIQRDPKVYMEPNEFRPERFITGEAKEFDVRGQNFELMPFGSGRRSCPGSSLAMQVL 485

[Close]

BLAST of Lsi04G016100 vs. TAIR 10
Match: AT4G31970.1 (cytochrome P450, family 82, subfamily C, polypeptide 2 )

HSP 1 Score: 362.1 bits (928), Expect = 6.6e-100
Identity = 198/529 (37.43%), Postives = 283/529 (53.50%), Query Frame = 0


Query: 5   FPSFNPISITMLVVLFFISCYYLWRKWAQHKVTEQKQAPAPPGAWPVIGHLHLLR-NAKL 64
           F  F PI + + + LF            + K  +  +APAP GAWP+IGHLHLL    +L
Sbjct: 6   FSLFVPILVFVFIALF-----------KKSKKPKHVKAPAPSGAWPIIGHLHLLSGKEQL 65

Query: 65  PHHALGAMADKYGPIFRLQLGSRSALVVSSWEMAKESMCVNDAAAASRPGVSGTKYFSYD 124
            +  LG MAD+YGP   L+LGS    VVSS+E+AK+   VND A ASRP  +  K+  YD
Sbjct: 66  LYRTLGKMADQYGPAMSLRLGSSETFVVSSFEVAKDCFTVNDKALASRPITAAAKHMGYD 125

Query: 125 FAAFGLAPYSPYWREIRKVTHMELLSNPRVDQVKSIMFGEINTSLTELYETWEAEKEESG 184
            A FG APYS +WRE+RK+  +ELLSN R+  +K +   EI+  + +LY  W  +K  S 
Sbjct: 126 CAVFGFAPYSAFWREMRKIATLELLSNRRLQMLKHVRVSEISMVMQDLYSLW-VKKGGSE 185

Query: 185 QVQVEMKRWFGNVIVNMLLKIIIGKRCVGPNAEGGE--KQAKDFQLAIRDSFHLMGQGLL 244
            V V++K W  ++ +NM+++++ GKR  G  +   E  ++A+  +  + + FHL+G   +
Sbjct: 186 PVMVDLKSWLEDMSLNMMVRMVAGKRYFGGGSLSPEDAEEARQCRKGVANFFHLVGIFTV 245

Query: 245 RDYIPIIGRLGFNGQVKVMKNIATRFDMVLSEWLEEHKR--------------------- 304
            D  P +G   F G  K MK      D++L  W+E H++                     
Sbjct: 246 SDAFPKLGWFDFQGHEKEMKQTGRELDVILERWIENHRQQRKVSGTKHNDSDFVDVMLSL 305

Query: 305 ------------------------------------------------------------ 364
                                                                       
Sbjct: 306 AEQGKFSHLQHDAITSIKSTCLALILGGSETSPSTLTWAISLLLNNKDMLKKAQDEIDIH 365

Query: 365 --RERRVKESDIGKLVYLQSIIKETMRMYPAGPLLGPREFYKDCVVAGYFVPKGTQLIPN 424
             R+R V++SDI  LVY+Q+IIKET+R+YPAGPLLG RE  +DC VAGY V +GT+++ N
Sbjct: 366 VGRDRNVEDSDIENLVYIQAIIKETLRLYPAGPLLGHREAIEDCTVAGYNVRRGTRMLVN 425

Query: 425 IWKIQTDSRVWPDPFEFKPERFLTSH-KDVDLKGNNFELIPFGSGRRGCPGIAFGLQMVH 447
           +WKIQ D RV+ +P EF+PERF+T   K+ D++G NFEL+PFGSGRR CPG +  +Q++H
Sbjct: 426 VWKIQRDPRVYMEPNEFRPERFITGEAKEFDVRGQNFELMPFGSGRRSCPGSSLAMQVLH 485

[Close]

BLAST of Lsi04G016100 vs. TAIR 10
Match: AT4G31950.1 (cytochrome P450, family 82, subfamily C, polypeptide 3 )

HSP 1 Score: 327.0 bits (837), Expect = 2.3e-89
Identity = 193/518 (37.26%), Postives = 267/518 (51.54%), Query Frame = 0


Query: 16  LVVLFFISCYYLWRKWAQHKVTEQKQAPAPPGAWPVIGHLHLL-RNAKLPHHALGAMADK 75
           ++V  FI+ +       + K  +  +APAP GAWP+IGHLHLL    +L +  LG MAD 
Sbjct: 12  ILVFVFIALF------KKSKKPKYVKAPAPSGAWPIIGHLHLLGGKEQLLYRTLGKMADH 71

Query: 76  YGPIFRLQLGSRSALVVSSWEMAKESMCVNDAAAASRPGVSGTKYFSYDFAAFGLAPYSP 135
           YGP   L+LGS    V SS+E+AK+   VND A AS    +  K+  Y F          
Sbjct: 72  YGPAMSLRLGSSETFVGSSFEVAKDCFTVNDKALASLM-TAAAKHMGYVF---------- 131

Query: 136 YWREIRKVTHMELLSNPRVDQVKSIMFGEINTSLTELYETWEAEKEESGQVQVEMKRWFG 195
            W E+RK+  +ELLSN R+  + ++   EI+  + +LY  W  +K  S  V V++K W  
Sbjct: 132 -WLEMRKIAMIELLSNRRLQMLNNVRVSEISMGVKDLYSLW-VKKGGSEPVMVDLKSWLE 191

Query: 196 NVIVNMLLKIIIGKRCV-GPNAEGGE--KQAKDFQLAIRDSFHLMGQGLLRDYIPIIGRL 255
           ++I NM+++++ GKR   G  AE  E  ++A+ ++  I   FHL+G   + D  P +G L
Sbjct: 192 DMIANMIMRMVAGKRYFGGGGAESSEHTEEARQWRKGIAKFFHLVGIFTVSDAFPKLGWL 251

Query: 256 GFNGQVKVMKNIATRFDMVLSEWLEEHKR------------------------------- 315
              G  K MK      D++L  W+E H++                               
Sbjct: 252 DLQGHEKEMKQTRRELDVILERWIENHRQQRKVSGTKHNDSDFVDVMLSLAEQGKLSHLQ 311

Query: 316 ----------------------------------------------------RERRVKES 375
                                                               R+R V++S
Sbjct: 312 YDANTCIKTTCLALILGGSETSPSTLTWAISLLLNNKDMLKKVQDEIDIHVGRDRNVEDS 371

Query: 376 DIGKLVYLQSIIKETMRMYPAGPLLGPREFYKDCVVAGYFVPKGTQLIPNIWKIQTDSRV 435
           DI  LVYLQ+IIKET+R+YPA PLLG RE  +DC VAGY VP GT+LI N+WKIQ D +V
Sbjct: 372 DIKNLVYLQAIIKETLRLYPAAPLLGHREAMEDCTVAGYNVPCGTRLIVNVWKIQRDPKV 431

Query: 436 WPDPFEFKPERFLTSH-KDVDLKGNNFELIPFGSGRRGCPGIAFGLQMVHFTLAGFLHSF 446
           + +P EF+PERF+T   KD D++G NFEL+PFGSGRR CPG +  +QM+H  LA FLHSF
Sbjct: 432 YMEPNEFRPERFITGEAKDFDVRGQNFELMPFGSGRRSCPGPSLAMQMLHLGLARFLHSF 491

[Close]

BLAST of Lsi04G016100 vs. TAIR 10
Match: AT2G25160.1 (cytochrome P450, family 82, subfamily F, polypeptide 1 )

HSP 1 Score: 289.3 bits (739), Expect = 5.4e-78
Identity = 172/521 (33.01%), Postives = 251/521 (48.18%), Query Frame = 0


Query: 16  LVVLFFISCYYLWR-----KWAQHKVTEQKQAPAPPGAWPVIGHLHLLRNAKLPHHALGA 75
           L++LF +S  +++      K       ++  AP  PGAWP++GHLHL       H   GA
Sbjct: 3   LIMLFLLSALFIFPVLILIKSRLRPKNKKSTAPMVPGAWPLLGHLHLFDTVNPTHVTFGA 62

Query: 76  MADKYGPIFRLQLGSRSALVVSSWEMAKESMCVNDAAAASRPGVSGTKYFSYDFAAFGLA 135
           MAD YGP+F  +LGS   ++++S E+AKE   V+D     RP ++ +K   Y+ +    +
Sbjct: 63  MADVYGPVFMAKLGSIKVMIINSKEVAKEIYTVHD-KLLERPELTASKLLGYNDSFLTFS 122

Query: 136 PYSPYWREIRKVTHMELLSNPRVDQVKSIMFGEINTSLTELYETWEAEKEESGQVQVEMK 195
           PY  YWREIRK+   EL S   VD        E + +   LY  WE   +    V V+MK
Sbjct: 123 PYGLYWREIRKIAVSELFSTSGVDMHMVSRAREADLAFRALYVRWEKRGKPKEGVLVDMK 182

Query: 196 RWFGNVIVNMLLKIIIGKRCVGPNAEGGEKQAKDFQLAIRDSFHLMGQGLLRDYIPIIGR 255
           + F ++  N+ L ++ GKR  G N     K+A+     IR+        LL D  P++G 
Sbjct: 183 QEFIDLTANISLMMVSGKRYFGENPNCEVKEARRCGKLIREFLDYFALFLLSDVAPVLGF 242

Query: 256 LGFNGQVKVMKNIATRFDMVLSEWLEEHKR------------------------------ 315
           L +  + + MK  A   D V   W+EEHK                               
Sbjct: 243 LDWKTK-RGMKRTAKGLDKVAEGWIEEHKNKRSDHGRSENDYLDILIKILGQDKIPGLSD 302

Query: 316 --------------------------------------------------RERRVKESDI 375
                                                             +ER V+E DI
Sbjct: 303 THTKIKALCLNLVLAGSETAIVVLVWAVSLLLNNPHVLRKAQEELDSKIGKERVVEELDI 362

Query: 376 GKLVYLQSIIKETMRMYPAGPLLGPREFYKDCVVA--GYFVPKGTQLIPNIWKIQTDSRV 435
             LVYLQ+I+KET R+YP  PL+  R   +D  +A     VP GTQL+ + WKI  D  V
Sbjct: 363 KDLVYLQAIVKETFRLYPPVPLVAYRAVVEDFDIAFCKCHVPAGTQLMVSAWKIHRDPNV 422

Query: 436 WPDPFEFKPERFLTSHKDVDLKGNNFELIPFGSGRRGCPGIAFGLQMVHFTLAGFLHSFD 450
           W +P +F+PERFLTS++++D+ G +++  PFG GRR CP I  G++MVH+ L  FLHSFD
Sbjct: 423 WSNPEQFEPERFLTSNRELDVGGQSYKFFPFGLGRRSCPAIPLGMRMVHYLLVRFLHSFD 482

[Close]

BLAST of Lsi04G016100 vs. TAIR 10
Match: AT3G25180.1 (cytochrome P450, family 82, subfamily G, polypeptide 1 )

HSP 1 Score: 285.0 bits (728), Expect = 1.0e-76
Identity = 174/522 (33.33%), Postives = 251/522 (48.08%), Query Frame = 0


Query: 8   FNPISITMLVVLFFISCYYLWRKWAQHKVTEQKQAPAPPGAWPVIGHLHLLRNAKLPHHA 67
           F+ + +++  +   I  Y   RK       +    P P GA P+ GHLHLLR  KL    
Sbjct: 5   FSTLQLSLFSLALVIFGYIFLRKQLSRCEVDSSTIPEPLGALPLFGHLHLLRGKKLLCKK 64

Query: 68  LGAMADKYGPIFRLQLGSRSALVVSSWEMAKESMCVNDAAAASRPGVSGTKYFSYDFAAF 127
           L AM+ K+GPIF L+LG    +V S  +  K+    ND A A+RP ++  +Y  Y+ A+ 
Sbjct: 65  LAAMSQKHGPIFSLKLGFYRLVVASDPKTVKDCFTTNDLATATRPNIAFGRYVGYNNASL 124

Query: 128 GLAPYSPYWREIRKVTHMELLSNPRVDQVKSIMFGEINTSLTELYETWEAEKEESGQVQV 187
            LAPY  YWRE+RK+  + L SN  ++ +  I   E+NT +  LY      K   G   V
Sbjct: 125 TLAPYGDYWRELRKIVTVHLFSNHSIEMLGHIRSSEVNTLIKHLY------KGNGGTSIV 184

Query: 188 EMKRWFGNVIVNMLLKIIIGKRCVGPNAEGGEKQAKDFQLAIRDSFHLMGQGLLRDYIPI 247
           ++   F  +  N++L+ ++GKR         E + K+   A++   +L    ++ D IP 
Sbjct: 185 KIDMLFEFLTFNIILRKMVGKRIGFGEVNSDEWRYKE---ALKHCEYLAVIPMIGDVIPW 244

Query: 248 IGRLGFNGQVKVMKNIATRFDMVLSEWLEEH-KRRERR---------------------- 307
           +G L F  +   MK +    D V ++WL EH K+R R                       
Sbjct: 245 LGWLDF-AKNSQMKRLFKELDSVNTKWLHEHLKKRSRNEKDQERTIMDLLLDILPEDIVI 304

Query: 308 ----------------------------------------------------------VK 367
                                                                     ++
Sbjct: 305 SGHVRDVIVKATILALTLTGSDSTSITLTWAVSLLLNNPAALEAAQEEIDNSVGKGRWIE 364

Query: 368 ESDIGKLVYLQSIIKETMRMYPAGPLLGPREFYKDCVVAGYFVPKGTQLIPNIWKIQTDS 427
           ESDI  L YLQ+I+KET R+YP  PL G RE  +DC V GY V KGT+L+ NIWK+  D 
Sbjct: 365 ESDIQNLKYLQAIVKETHRLYPPAPLTGIREAREDCFVGGYRVEKGTRLLVNIWKLHRDP 424

Query: 428 RVWPDPFEFKPERFLTSHKDVDLKGNNFELIPFGSGRRGCPGIAFGLQMVHFTLAGFLHS 449
           ++WPDP  FKPERF+      +   +NFE IPFGSGRR CPG+  GL++VHF LA  L  
Sbjct: 425 KIWPDPKTFKPERFMEDKSQCE--KSNFEYIPFGSGRRSCPGVNLGLRVVHFVLARLLQG 484

The following BLAST results are available for this feature:

BLAST of Lsi04G016100 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O49858	7.8e-114	40.89	Cytochrome P450 82A3 OS=Glycine max OX=3847 GN=CYP82A3 PE=2 SV=1	[more]
O49859	3.9e-113	41.04	Cytochrome P450 82A4 OS=Glycine max OX=3847 GN=CYP82A4 PE=2 SV=1	[more]
Q43068	3.2e-107	38.04	Cytochrome P450 82A1 (Fragment) OS=Pisum sativum OX=3888 GN=CYP82A1 PE=2 SV=2	[more]
Q9SZ46	2.0e-101	38.56	Xanthotoxin 5-hydroxylase CYP82C4 OS=Arabidopsis thaliana OX=3702 GN=CYP82C4 PE=...	[more]
O81972	7.1e-99	37.77	Cytochrome P450 82A2 OS=Glycine max OX=3847 GN=CYP82A2 PE=2 SV=1	[more]

BLAST of Lsi04G016100 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LD43	9.6e-232	75.14	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G852590 PE=3 SV=1	[more]
A0A1S3CMR1	1.1e-227	74.01	cytochrome P450 CYP82D47-like OS=Cucumis melo OX=3656 GN=LOC103502568 PE=3 SV=1	[more]
A0A6J1FBI1	3.3e-224	74.01	cytochrome P450 82A3-like OS=Cucurbita moschata OX=3662 GN=LOC111442563 PE=3 SV=...	[more]
A0A6J1IGS1	4.9e-220	73.26	cytochrome P450 82A3-like OS=Cucurbita maxima OX=3661 GN=LOC111476809 PE=3 SV=1	[more]
A0A6J1DJE5	3.3e-200	66.67	cytochrome P450 82A3-like OS=Momordica charantia OX=3673 GN=LOC111021426 PE=3 SV...	[more]

BLAST of Lsi04G016100 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004147824.1	2.0e-231	75.14	cytochrome P450 CYP82D47 [Cucumis sativus] >KGN59910.1 hypothetical protein Csa_...	[more]
XP_038897047.1	1.8e-229	75.14	cytochrome P450 CYP82D47-like [Benincasa hispida]	[more]
XP_008464762.1	2.3e-227	74.01	PREDICTED: cytochrome P450 CYP82D47-like [Cucumis melo]	[more]
XP_022935740.1	6.8e-224	74.01	cytochrome P450 82A3-like [Cucurbita moschata]	[more]
XP_023535455.1	5.4e-221	73.26	cytochrome P450 82A3-like [Cucurbita pepo subsp. pepo]	[more]

BLAST of Lsi04G016100 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G31940.1	1.4e-102	38.56	cytochrome P450, family 82, subfamily C, polypeptide 4	[more]
AT4G31970.1	6.6e-100	37.43	cytochrome P450, family 82, subfamily C, polypeptide 2	[more]
AT4G31950.1	2.3e-89	37.26	cytochrome P450, family 82, subfamily C, polypeptide 3	[more]
AT2G25160.1	5.4e-78	33.01	cytochrome P450, family 82, subfamily F, polypeptide 1	[more]
AT3G25180.1	1.0e-76	33.33	cytochrome P450, family 82, subfamily G, polypeptide 1	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002401	Cytochrome P450, E-class, group I	PRINTS	PR00463	EP450I	coord: 297..315 score: 31.8 coord: 386..409 score: 23.93 coord: 376..386 score: 53.79 coord: 72..91 score: 37.09 coord: 338..362 score: 39.52
IPR036396	Cytochrome P450 superfamily	GENE3D	1.10.630.10	Cytochrome P450	coord: 284..445 e-value: 6.0E-50 score: 172.1 coord: 32..283 e-value: 9.5E-36 score: 125.3
IPR036396	Cytochrome P450 superfamily	SUPERFAMILY	48264	Cytochrome P450	coord: 43..440
IPR001128	Cytochrome P450	PFAM	PF00067	p450	coord: 281..426 e-value: 1.0E-42 score: 146.5 coord: 43..281 e-value: 2.7E-22 score: 79.2
None	No IPR available	PANTHER	PTHR47947:SF26	CYTOCHROME P450	coord: 39..281 coord: 281..445
None	No IPR available	PANTHER	PTHR47947	CYTOCHROME P450 82C3-RELATED	coord: 39..281 coord: 281..445
IPR017972	Cytochrome P450, conserved site	PROSITE	PS00086	CYTOCHROME_P450	coord: 379..388

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi04G016100.1	Lsi04G016100.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0098542	defense response to other organism
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0004497	monooxygenase activity
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen