Lsi04G013960 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi04G013960
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Descriptionprotein BIG GRAIN 1-like B
Locationchr04: 21756387 .. 21764747 (-)
RNA-Seq ExpressionLsi04G013960
SyntenyLsi04G013960
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAGCGGGGAGGAAGTAAGGGCGAGAGGTGAGAGGTTTAGGAATGGAGGAGGTAATGGACAGTCATTTTCTTCGAGTTTGCTTGATGAAATCTACCGTTCGATTGATGATGGAGGTGATAAGAGAAGTGGAGAATTGAAGTTTTACAGACAGAAGGTGTTGAAGAAACAAGGTAAAACTATCGGGAAAGCGCAGAGTGATGTTGAAGATGAGGAGATTGCGAGTCTTCATCGTGCTTTGTTGATCGAGAGATGGATCGAGAAGAAGGTTGCTGAAAAGGTGAGCGCTCAAAGGAGACGATCTCTGACGGAAGCTGAGATAACATTTCAGTTGTATCAGCATGATCGAGAAGAAGACGTTCTGTTCTTCAGTTCAACTTCGAGTTCTTCCGATTCGAGCTTCGGTGGATTTTCATCTTCGGATACTGAGTCAATGTATGGTTCGAAATCCTTGACGCCGTCTTGTTTCGCGAAGTTCAGGCCGAAGCCGGTTCGGACCAGCGTCTCGGCTCCACCGCCGGAGAAGATTGAGGCAAAGCAGAGACAGAGTAGAGAGAAGCCACAGTCCAAACAGAGCAATCAGTTCGTTGAAACCAAAGAAAAATCCGGAGGTTTCGACGATAACGCCATGATTAAATCGAAATCTAGGGCGTTGAAGATTTACAGTAATTTAAAGAAGGTGAAGCAACCAATTTCACCAGGAGGACGCCTCTCAAGTTTTCTCAACTCAATCTTCACTGCAGGAACTCCAAAGAAGACTACAAACTCCGTATCATCAACCGGCGTCTCTGAAGATCCAAACTCAGAAAGAAAATCAAAATCCGGCCAAACTTCCACTTGTTCTTCCGCCGCATCGTTTTCCAGATCATGTTTAAGCAAAAGCTCACCATGCTCTAGCGAAAAATTACGCAATGGCGACAGAAGAACGGTTCGATTCTATCCCGTGAGTACAATCGTAGACGAATACTGTAGACCTTGCGGACACAAATCCTTATACGAAGAAGATCCTAAATTCCAAATCAAGCACCAAGCCGAAACCGCCAAATCAACAACAACAGCAGCCAGAAAAATTAAAACAGATTATCAGCAAATCAGGAAGAAGAACGATTTCCTGATTAGAAATTTTCACCATCCCGACAACAATCTCTACGAAGAAGAAGAAGACGACGACGAAACAGCAAGTTGTTCAAGTTCAGATCTGTTTGAACTAGATCATCTGAGGGTAATGAGCGGCGGCGATCGATACCGTGAAGAGCTTCCGGTGTACGAAACAACCACTCGTGTGGATACAAATCGAGTCATCCGCCATTGCTAATTAGGTTTTAATTCCATTTGTAATAGGTATTGATTATTATTATTATTATTCAGCCATAAAATTTGTTATAGCGATGTGTTTGTTTGTTCATATTGATGATATTCAGTTATGAGGGTTTTGGGGAAATGGATTTGGAAACATTAGAAAGAAAAGGAAAAGACAGAGGAAAGGAAAGCGTGTGGGAACGTTAAAAAAATTTCAGAAAAAGAAAGATTGTGGGGATAAGTCTTTGACAGTTTGATTTTGATGGGAAGGGAAAGTAAAGAAGAGAAGAAGGAAGGGTATAAATTTAGGAAGAGATAAAAAATAAATAAAGATTGTGAGATTGGAGGATGAGAATAGAAGAAGCAAAAATGAGAGACAAAGAAAGAGAATGTGATGGTAAAGGCAAAGGCAGATCTTCGTCCCCATGGGAACAGAGAAAGTGAAGATTTTCAAACCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTGGGTTGTTTTAATTTTGTCTGCAATTTCTTAAGTATTGGCCCCTCTTGTTACTTCCTTTCCCATCCCAATCAAACATCTTCTTTTTCTTTTTCCTTTTTTTTCCCCAAAATAAAATACACCATATGCACATTCATTCTCATTCATTTTTCTTACTATTATAATCTTAGATGATATAATATTAATTTATTTTATGTCAATCTCTGATTTAAGAATATAATTAAGTTTAAATACTACTTTAAACTCTATACTCATTTTAGTCCTTATACTTTCAAATTCTTCATTTTGGTCTCTATACTTTCAACTTTAATTCATTTTGGTCCCGTTCATTTTGATTCTTATACTTTTAAAAAGTTGTCATTTTGGCCTCTTTATTTTCATTTTTAAATGATAAAAATAGTCATTTTTTTTAAGTTCAAGGACCAAAATGAACCAAAGTTGAAAGTACAATGATCAAAATGAACATTTTTAAAGTATAGGGACCAAAATGAACAAAAACAAAAAGTACAATAACCAAAGTAGTACTTAAACCATATAATTAATACAATTCTTTTTCAAAGTATAAAATTATAATTGTGGGAGTTTAGGAAAATTACTATAAATAGAACAGTGAAATTTTTCTATATTTGTAAATATTTTGATATTTTTTCTATATTAGGAAAAATTGCCTGAGAGTTTTTTTTTTTTTTAAATTATTGAAATTCAACAATAGGCAGTGGGGATCGGAATGATTTAAAATTAAAAAAAAGAAAAAAAAAAGAAAAATTACCTTTAGTTTTGAAAAATAGGAAAAAGAATTCGCGCCAGGTAGGGGTCGAACCTACGACTTTCTGCTTAGGAAACAGACGCTCTATCCACTGAGCTACAGGCGCCTTATGATTATTTAAAATGTATATTTATCTTCTAAACTTTTAAAATTAAAATTTTAATTATAATCATTCAATTTTGGTAAATATCTGATCTAATCCTTTCAATATAGTAATGATCTAAATTTGGCATGTAAAATAATAATTAATATTACAAAATTGAATTCTTTCAAATTTTTAATTTTTAATTATATTCCTCCAATTTTTTTCAATTTCTTATATGGATCTTCTAGTAATATTAATTATCATAATTTGACATGTAAATAATAGGCAATATTAAAGCATAAATTTCCTTAAATTCTTGAACTTTTAATTATAATCCTCCAATTTTGTTATTTAAACCTTCTAGTAATAGTGCTTATCTAAATTAATATTACCACATCATTTTCCTTAAATTTTTTTATTTTTAATTATAATCCTCCAATTTTGTTAAATATCTTATTCGAACCTTCTAGTAATAGTGATAATCTAAATTTGATATGTAAACAACAATTAATATTACCACATCATTTTCCTTAAATTTTTTTAACTTTTAATTAAATTTTTTTAACTTTTAATTATAATTCTCCATTTTTGTTAAATATCATATGGTAAGCAATGGAAATAATCAATTTTTTTTTTTTTTTGTAGATTCCAACAAATATTCTATTTACTCCTTTTTTTTTTTTTTTTTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN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mRNA sequence

ATGAGCGGGGAGGAAGTAAGGGCGAGAGGTGAGAGGTTTAGGAATGGAGGAGGTAATGGACAGTCATTTTCTTCGAGTTTGCTTGATGAAATCTACCGTTCGATTGATGATGGAGGTGATAAGAGAAGTGGAGAATTGAAGTTTTACAGACAGAAGGTGTTGAAGAAACAAGGTAAAACTATCGGGAAAGCGCAGAGTGATGTTGAAGATGAGGAGATTGCGAGTCTTCATCGTGCTTTGTTGATCGAGAGATGGATCGAGAAGAAGGTTGCTGAAAAGGTGAGCGCTCAAAGGAGACGATCTCTGACGGAAGCTGAGATAACATTTCAGTTGTATCAGCATGATCGAGAAGAAGACGTTCTGTTCTTCAGTTCAACTTCGAGTTCTTCCGATTCGAGCTTCGGTGGATTTTCATCTTCGGATACTGAGTCAATGTATGGTTCGAAATCCTTGACGCCGTCTTGTTTCGCGAAGTTCAGGCCGAAGCCGGTTCGGACCAGCGTCTCGGCTCCACCGCCGGAGAAGATTGAGGCAAAGCAGAGACAGAGTAGAGAGAAGCCACAGTCCAAACAGAGCAATCAGTTCGTTGAAACCAAAGAAAAATCCGGAGGTTTCGACGATAACGCCATGATTAAATCGAAATCTAGGGCGTTGAAGATTTACAGTAATTTAAAGAAGGTGAAGCAACCAATTTCACCAGGAGGACGCCTCTCAAGTTTTCTCAACTCAATCTTCACTGCAGGAACTCCAAAGAAGACTACAAACTCCGTATCATCAACCGGCGTCTCTGAAGATCCAAACTCAGAAAGAAAATCAAAATCCGGCCAAACTTCCACTTGTTCTTCCGCCGCATCGTTTTCCAGATCATGTTTAAGCAAAAGCTCACCATGCTCTAGCGAAAAATTACGCAATGGCGACAGAAGAACGGTTCGATTCTATCCCGTGAGTACAATCGTAGACGAATACTGTAGACCTTGCGGACACAAATCCTTATACGAAGAAGATCCTAAATTCCAAATCAAGCACCAAGCCGAAACCGCCAAATCAACAACAACAGCAGCCAGAAAAATTAAAACAGATTATCAGCAAATCAGGAAGAAGAACGATTTCCTGATTAGAAATTTTCACCATCCCGACAACAATCTCTACGAAGAAGAAGAAGACGACGACGAAACAGCAAGTTGTTCAAGTTCAGATCTGTTTGAACTAGATCATCTGAGGGTAATGAGCGGCGGCGATCGATACCGTGAAGAGCTTCCGGTTTTGATTTTGATGGGAAGGGAAAAGAAAGATCAATGGGTTCTTATTTCAAAATTATTAGGAGAGATGAGATCAATCATCAATCACATGGAAATGAACGTTAAGTGTTTGATAAAATACCCCATCAAAGCGTTAACTTCATGGACATCATTGGCATCAACGCCTTTCCCTCCATGTGCAGGCTTGTGTAGAAAGGGACTTCATTTCACCTTTCTTCTTTTCTTATGGTATGAAATTGACAGCATTATCTTCAAGAAGTCCGCCAAATATTTCGTCTCCAATCACTAA

Coding sequence (CDS)

ATGAGCGGGGAGGAAGTAAGGGCGAGAGGTGAGAGGTTTAGGAATGGAGGAGGTAATGGACAGTCATTTTCTTCGAGTTTGCTTGATGAAATCTACCGTTCGATTGATGATGGAGGTGATAAGAGAAGTGGAGAATTGAAGTTTTACAGACAGAAGGTGTTGAAGAAACAAGGTAAAACTATCGGGAAAGCGCAGAGTGATGTTGAAGATGAGGAGATTGCGAGTCTTCATCGTGCTTTGTTGATCGAGAGATGGATCGAGAAGAAGGTTGCTGAAAAGGTGAGCGCTCAAAGGAGACGATCTCTGACGGAAGCTGAGATAACATTTCAGTTGTATCAGCATGATCGAGAAGAAGACGTTCTGTTCTTCAGTTCAACTTCGAGTTCTTCCGATTCGAGCTTCGGTGGATTTTCATCTTCGGATACTGAGTCAATGTATGGTTCGAAATCCTTGACGCCGTCTTGTTTCGCGAAGTTCAGGCCGAAGCCGGTTCGGACCAGCGTCTCGGCTCCACCGCCGGAGAAGATTGAGGCAAAGCAGAGACAGAGTAGAGAGAAGCCACAGTCCAAACAGAGCAATCAGTTCGTTGAAACCAAAGAAAAATCCGGAGGTTTCGACGATAACGCCATGATTAAATCGAAATCTAGGGCGTTGAAGATTTACAGTAATTTAAAGAAGGTGAAGCAACCAATTTCACCAGGAGGACGCCTCTCAAGTTTTCTCAACTCAATCTTCACTGCAGGAACTCCAAAGAAGACTACAAACTCCGTATCATCAACCGGCGTCTCTGAAGATCCAAACTCAGAAAGAAAATCAAAATCCGGCCAAACTTCCACTTGTTCTTCCGCCGCATCGTTTTCCAGATCATGTTTAAGCAAAAGCTCACCATGCTCTAGCGAAAAATTACGCAATGGCGACAGAAGAACGGTTCGATTCTATCCCGTGAGTACAATCGTAGACGAATACTGTAGACCTTGCGGACACAAATCCTTATACGAAGAAGATCCTAAATTCCAAATCAAGCACCAAGCCGAAACCGCCAAATCAACAACAACAGCAGCCAGAAAAATTAAAACAGATTATCAGCAAATCAGGAAGAAGAACGATTTCCTGATTAGAAATTTTCACCATCCCGACAACAATCTCTACGAAGAAGAAGAAGACGACGACGAAACAGCAAGTTGTTCAAGTTCAGATCTGTTTGAACTAGATCATCTGAGGGTAATGAGCGGCGGCGATCGATACCGTGAAGAGCTTCCGGTTTTGATTTTGATGGGAAGGGAAAAGAAAGATCAATGGGTTCTTATTTCAAAATTATTAGGAGAGATGAGATCAATCATCAATCACATGGAAATGAACGTTAAGTGTTTGATAAAATACCCCATCAAAGCGTTAACTTCATGGACATCATTGGCATCAACGCCTTTCCCTCCATGTGCAGGCTTGTGTAGAAAGGGACTTCATTTCACCTTTCTTCTTTTCTTATGGTATGAAATTGACAGCATTATCTTCAAGAAGTCCGCCAAATATTTCGTCTCCAATCACTAA

Protein sequence

MSGEEVRARGERFRNGGGNGQSFSSSLLDEIYRSIDDGGDKRSGELKFYRQKVLKKQGKTIGKAQSDVEDEEIASLHRALLIERWIEKKVAEKVSAQRRRSLTEAEITFQLYQHDREEDVLFFSSTSSSSDSSFGGFSSSDTESMYGSKSLTPSCFAKFRPKPVRTSVSAPPPEKIEAKQRQSREKPQSKQSNQFVETKEKSGGFDDNAMIKSKSRALKIYSNLKKVKQPISPGGRLSSFLNSIFTAGTPKKTTNSVSSTGVSEDPNSERKSKSGQTSTCSSAASFSRSCLSKSSPCSSEKLRNGDRRTVRFYPVSTIVDEYCRPCGHKSLYEEDPKFQIKHQAETAKSTTTAARKIKTDYQQIRKKNDFLIRNFHHPDNNLYEEEEDDDETASCSSSDLFELDHLRVMSGGDRYREELPVLILMGREKKDQWVLISKLLGEMRSIINHMEMNVKCLIKYPIKALTSWTSLASTPFPPCAGLCRKGLHFTFLLFLWYEIDSIIFKKSAKYFVSNH
Homology
BLAST of Lsi04G013960 vs. ExPASy Swiss-Prot
Match: Q9SLL2 (Protein BIG GRAIN 1-like B OS=Arabidopsis thaliana OX=3702 GN=At1g54200 PE=1 SV=1)

HSP 1 Score: 162.9 bits (411), Expect = 9.5e-39
Identity = 159/409 (38.88%), Postives = 210/409 (51.34%), Query Frame = 0

Query: 22  SFSSSLLDEIYRSIDDGGDKRSGELKFYRQKVLKKQGKTIGKAQSDVEDEEIASLHRALL 81
           SFSS+LLD+IYRSIDD     S   K   Q                              
Sbjct: 27  SFSSTLLDQIYRSIDDSSTNSSSMRKTKHQ------------------------------ 86

Query: 82  IERWIEKKVAEKVSAQRRRSLTEAEITFQLYQHDREEDVLFFSSTSSSSDSSFGGFSSSD 141
                  +   +VSA RR     ++    +      E V F  S+SSSSDSS  GFSSS+
Sbjct: 87  ------NREDTRVSANRRDDFNRSKNLKTI------EPVFFKHSSSSSSDSS--GFSSSE 146

Query: 142 TESMYGSKSLTPSCFAKFRPKPVRTSVSAPPPEKIEAKQRQSREKPQSKQSNQFVETKEK 201
           ++  Y     +P   A   PKP+RT+V     E+ E         PQ+ + N     K++
Sbjct: 147 SDYFYRRSKSSP---AISHPKPIRTTV-----ERFE-------RSPQNHRPNS--SNKQE 206

Query: 202 SGGFDDNAMIKSKSRALKIYSNLKKVKQPISPGGRLSSFLNSIFTAGTPKKTTNSVSSTG 261
            G F     +K+KS+ALKIYS+LKKVKQPISPGGRL++FLNSIFT     K  N +++T 
Sbjct: 207 HGSF-----LKTKSKALKIYSDLKKVKQPISPGGRLATFLNSIFTGAGNTKKLNKINTTV 266

Query: 262 VSEDPNSERKSKSGQTSTCSSAASFSRSCLSKSSPCSSEKLRNGDRRTVRFYPVSTIVDE 321
            S    +   + +  T+TCSSA+SFSRSCLSK +P SSEK     +R+VRF PV+ I DE
Sbjct: 267 TS---TTAAAAAASSTTTCSSASSFSRSCLSK-TPSSSEK----SKRSVRFCPVNVIFDE 326

Query: 322 YCRPCGHKS--LY--EEDPKFQIKHQAETA-----KSTTTAARKIKTDYQQIRKKNDFLI 381
                 +K+  +Y   E     I+H  E       +    AA+++   YQ   KKN  +I
Sbjct: 327 DSSKYNNKNNKVYGNNEREYESIRHTLENRVMEENRRVIEAAKELLRSYQ---KKNKEVI 352

Query: 382 RNFHHPDNNLYEEEEDDDETASCSSSDLFELDHLRVMSGGDRYREELPV 422
                 D     EE+DDD+  SC+SSDLFELD+L  + G DRYREELPV
Sbjct: 387 EVSVEDD-----EEDDDDDALSCTSSDLFELDNLSAI-GIDRYREELPV 352

BLAST of Lsi04G013960 vs. ExPASy Swiss-Prot
Match: Q9LVK2 (Protein BIG GRAIN 1-like A OS=Arabidopsis thaliana OX=3702 GN=At3g13980 PE=3 SV=1)

HSP 1 Score: 159.5 bits (402), Expect = 1.0e-37
Identity = 149/410 (36.34%), Postives = 215/410 (52.44%), Query Frame = 0

Query: 22  SFSSSLLDEIYRSIDDGGDKRSGELKFYRQKVLKKQGKTIGKAQSDVEDEEIASLHRALL 81
           SFSS+LLD+IYRSIDD       E       + KK+     +  S  ED EI+ ++    
Sbjct: 18  SFSSTLLDQIYRSIDDSSPPPPLE------SIKKKKHHHQQRNASLHEDREISPIY---- 77

Query: 82  IERWIEKKVAEKVSAQRRRSLTEAEITFQLYQHDREEDVLFFSSTSSSSDSSFGGFSSSD 141
                            RRS+         ++  R +      S SSSSDSS  GFSSS+
Sbjct: 78  ----------------HRRSIAAD------FERSRRKTDFLRHSNSSSSDSS--GFSSSE 137

Query: 142 TESMYG--SKSLTPSCFAKFRPKPVRTSVSAPPPEKIEAKQRQSREKPQSKQSNQFVETK 201
           ++S +G    S +P   ++ +PKP+RTS                     S   +  V+  
Sbjct: 138 SDSFHGRSKSSASPPSSSRQQPKPIRTS---------------------SVDHSSAVQKP 197

Query: 202 EKSGGFDDNAMIKSKSRALKIYSNLKKVKQPISPGGRLSSFLNSIFT-AGTPKKTTNSVS 261
           ++ GGF     +++KS+ALKIYS+LKKVKQPISPGGRL++FLNS+FT A T  K     +
Sbjct: 198 KELGGF-----LRTKSKALKIYSDLKKVKQPISPGGRLATFLNSLFTNAATNPKKHKKTT 257

Query: 262 STGVSEDPNSERKSKSGQTSTCSSAASFSRSCLSKSSPCSSEKLRNGDRRTVRFYPVSTI 321
           +  V E+P+S        +STCSSA+SFSRSCLSK +P SS K     +R+VRF PV+ I
Sbjct: 258 TVAVVEEPHS--------SSTCSSASSFSRSCLSK-TPSSSGK----SKRSVRFCPVNVI 317

Query: 322 VDEYCRPCGHKSLYEEDPKFQIKHQAETAKSTTTAARKIKTDYQQIRKKNDFLIRNFHHP 381
           +D             ED  F + +     +       K   +++++ +    L+R +H+ 
Sbjct: 318 LD-------------EDSSFTMPYAYNNERLYDNNEAKRVEEHRRVIQAAKDLLRTYHNK 338

Query: 382 D-------NNLYEEEEDDDETASCSSSDLFELDHLRVMSGGDRYREELPV 422
           +       NN+  EE+D+D+ ASC+SSDLFEL++L  + G +RYREELPV
Sbjct: 378 NKVTTTNINNV--EEDDEDDAASCASSDLFELENLSAI-GIERYREELPV 338

BLAST of Lsi04G013960 vs. ExPASy Swiss-Prot
Match: Q9LYH0 (Protein BIG GRAIN 1-like D OS=Arabidopsis thaliana OX=3702 GN=At5g12050 PE=2 SV=1)

HSP 1 Score: 99.8 bits (247), Expect = 9.8e-20
Identity = 109/334 (32.63%), Postives = 161/334 (48.20%), Query Frame = 0

Query: 98  RRRSLTEAEITFQLYQHDREEDVLFFSSTSSSSDSSFGGFSSSDTESMYGSK--SLTPSC 157
           +++S+    +  +    DR     FF S SSSSDS+   FSSSDTE  +G K  S  P C
Sbjct: 41  KKQSIVTRSVPDRKIHRDR-----FFGSVSSSSDSNSSIFSSSDTELTHGKKTTSSRPLC 100

Query: 158 FAKFRPKPVRTSVSAPPPEKIEAKQRQSREKPQSKQSNQFVETKEKSGGFDDNAMIKSKS 217
           F   + KP +T       +K    Q ++    +      +     K    D+N       
Sbjct: 101 FGPSKTKPRKTE------DKTLFHQNRA---TRVYDDYDYASDVPKFNRHDENWENTRNR 160

Query: 218 RALKIYSNLKKVKQPISPGGRLSSFLNSIFTAGTPKKTTNSVSS----TGVSEDPNSERK 277
           R++K   N KK K P SPGGR+ +FLNS+F+  +  K +N+V S    T   +     + 
Sbjct: 161 RSVKSSGNQKKPKTPASPGGRIVNFLNSLFSNNS--KQSNAVKSYPRKTSYDDSAYVRKT 220

Query: 278 SKS--GQTSTCSSAASFSRSCLSKSSPCSSEKLRNGDRRTVRFYPVSTIVDEYCRPCGHK 337
           S      T+TCSSA+SFSRSC++K    SS ++    +R+VRF PV+ IV E        
Sbjct: 221 SNDYHSSTTTCSSASSFSRSCMNKGYEKSSGRI----KRSVRFSPVNVIVPESFTSKEED 280

Query: 338 SLYEEDPKFQIKHQAETA--KSTTTAARKIKTDYQQIRKKNDFLIRNFHHPDNNLYEEEE 397
                + +  +K   E    +S    AR+   DY   +   + L++     D    +E++
Sbjct: 281 YFSNGNARKSVKKNVEDGGRRSVEEIAREFLRDYH--KNHENSLVKTNGFEDYEDDDEDD 340

Query: 398 DDDETASCSSSDLFELDHLRVMSGGDRYREELPV 422
           DDD+ AS SSSDLFELD +      + Y +ELPV
Sbjct: 341 DDDDVASDSSSDLFELDLVGNHHHHNVYGDELPV 352

BLAST of Lsi04G013960 vs. ExPASy Swiss-Prot
Match: Q9M2B3 (Protein BIG GRAIN 1-like C OS=Arabidopsis thaliana OX=3702 GN=At3g42800 PE=2 SV=1)

HSP 1 Score: 84.7 bits (208), Expect = 3.3e-15
Identity = 113/410 (27.56%), Postives = 178/410 (43.41%), Query Frame = 0

Query: 22  SFSSSLLDEIYRSIDDGGDKRSGELKFYRQKVLKKQGKTIGKAQSDVEDEEIASLHRALL 81
           SFSSS+LD +YRSID+  D    +LK     + +    +      + +D+++ +L RA++
Sbjct: 11  SFSSSVLDSVYRSIDE-SDGLQSDLK---GSINENVSSSSSSPSPNKKDDKLTTLRRAIM 70

Query: 82  IERWIEKKVAEKVSAQRRRSLTEAEITFQLYQHDREEDVLFFSSTSSSSDSSFGGFSSSD 141
                                            D E  +   SST++++ S    FSSS+
Sbjct: 71  ---------------------------------DEEHWLYARSSTTTTNSSDSSSFSSSE 130

Query: 142 TESMYGSKSLTPSCFAKFRPKPVRTSVSAPPPEKIEAKQRQSREKPQSKQSNQFVETKEK 201
            ES    + L                      +  E  +R   E+ ++K++    +++  
Sbjct: 131 AESYRTKRRLR---------------------KLAEQGKRSGDERQRTKRTVMDNDSRLF 190

Query: 202 SGGFDDNAMIKSKSRALKIYSNLKKVKQPISPGGRLSSFLNSIFTAGTPKKTTNSVSSTG 261
           S   DD      K +A+KI   LK+ KQP+SPG RL+SFLNSIF +   K    SV  T 
Sbjct: 191 SKSDDD-----KKPKAVKIIEELKRSKQPVSPGARLTSFLNSIFQSNAKKVKLCSVGKT- 250

Query: 262 VSEDPNSERKSKSGQTSTCSSAASFSRSCLSKSSPCSSEKLRNGDR--RTVRFYPVSTIV 321
                 ++ KS S            S+SC S++   +     N  +  R++RFYPV   +
Sbjct: 251 ------TDVKSSS------------SKSCFSRTRNKTDNNNNNCKKLERSIRFYPVRVTI 310

Query: 322 DEYCRPCGHKSL---YEEDPKFQIKHQAETAKSTTTAARKIKTDYQQIRKKNDF--LIRN 381
           D  CR    K +    +  P+F             TA + +K + +      +F  + RN
Sbjct: 311 DGDCRDYAQKHITRVRKPIPEF-------------TAKKSVKEEIKTNDHHTEFTCITRN 323

Query: 382 FHHPD---NNLYEEEEDDDETASCSSSDLFELDHLRVMSGGDRYREELPV 422
               D   +N YE +E++++  S SSSDLFELD  R+  G  RY +ELPV
Sbjct: 371 IGLKDFVRSNKYEGKEEEEDAWSHSSSDLFELDSYRI--GMGRYLKELPV 323

BLAST of Lsi04G013960 vs. ExPASy TrEMBL
Match: A0A0A0L438 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G646410 PE=3 SV=1)

HSP 1 Score: 698.7 bits (1802), Expect = 1.8e-197
Identity = 389/427 (91.10%), Postives = 400/427 (93.68%), Query Frame = 0

Query: 1   MSGEEVRAR-GERFRNGG-GNGQSFSSSLLDEIYRSIDDGGDKRSGELKFYRQKVLKKQG 60
           MSGEEVR R GERFRNGG GNGQSFSSSLLDEIYRSIDDGGDKRSGELKFYRQKVLKKQG
Sbjct: 1   MSGEEVRVRSGERFRNGGCGNGQSFSSSLLDEIYRSIDDGGDKRSGELKFYRQKVLKKQG 60

Query: 61  KTIGKAQSDVEDEEIASLHRALLIERWIEKKVAEKVSAQRRRSLTEAEITFQLYQHDREE 120
           K IGKAQSDVEDEEIASLHRALLIERWIEKKVAEKVSAQRRRSLTE EI FQLYQHDREE
Sbjct: 61  KVIGKAQSDVEDEEIASLHRALLIERWIEKKVAEKVSAQRRRSLTEGEIKFQLYQHDREE 120

Query: 121 DVLFFSSTSSSSDSSFGGFSSSDTESMYGSKSLTPSCFAKFRPKPVRTSVSAPPPEKIEA 180
           DVLFFSSTSSSSDSSFGGFSSSDTESMYGSKSLTPSCFAKFRPKP+RTSVSAPPPEK EA
Sbjct: 121 DVLFFSSTSSSSDSSFGGFSSSDTESMYGSKSLTPSCFAKFRPKPIRTSVSAPPPEKTEA 180

Query: 181 KQRQSREKPQSKQSNQFVETKEKSGGFDDNAMIKSKSRALKIYSNLKKVKQPISPGGRLS 240
           KQRQSREKPQSKQSNQFVE KEKS GFD+NAMIKSKSRALKIYSNLKKVKQPISPGGRLS
Sbjct: 181 KQRQSREKPQSKQSNQFVENKEKSRGFDENAMIKSKSRALKIYSNLKKVKQPISPGGRLS 240

Query: 241 SFLNSIFTAGTPKKTTNSVSSTGVSEDPNSERKSKSGQTSTCSSAASFSRSCLSKSSPCS 300
           SFLNSIFTAGTPKKT NSVSST +SEDPNSERKSKSGQTSTCSSA SFSRSCLSKSSPCS
Sbjct: 241 SFLNSIFTAGTPKKTGNSVSSTTLSEDPNSERKSKSGQTSTCSSATSFSRSCLSKSSPCS 300

Query: 301 SEKLRNGDRRTVRFYPVSTIVDEYCRPCGHKSLY-EEDPKFQIKHQAETAKSTTTAARKI 360
           SEKLRN DRRTVRFYPVSTIVDEYCRPCGHKSLY EEDPKFQIK++AE+A+ST   +RK 
Sbjct: 301 SEKLRNADRRTVRFYPVSTIVDEYCRPCGHKSLYEEEDPKFQIKNKAESAEST---SRKN 360

Query: 361 KTDY-QQIRKKNDFLIRNFHHPDNNLYEEEEDDDETASCSSSDLFELDHLRVMSGG--DR 420
            TDY QQ+RKKNDFLIRNFHHPDN+L EE   DDETASCSSSDLFELDHLR MSGG  +R
Sbjct: 361 TTDYQQQMRKKNDFLIRNFHHPDNDLSEE---DDETASCSSSDLFELDHLREMSGGCSNR 420

Query: 421 YREELPV 422
           YREELPV
Sbjct: 421 YREELPV 421

BLAST of Lsi04G013960 vs. ExPASy TrEMBL
Match: A0A6J1FE05 (protein BIG GRAIN 1-like B OS=Cucurbita moschata OX=3662 GN=LOC111443225 PE=3 SV=1)

HSP 1 Score: 692.6 bits (1786), Expect = 1.3e-195
Identity = 376/421 (89.31%), Postives = 395/421 (93.82%), Query Frame = 0

Query: 1   MSGEEVRARGERFRNGGGNGQSFSSSLLDEIYRSIDDGGDKRSGELKFYRQKVLKKQGKT 60
           MSG+EVRARGERFR+GGG  QSFSSSLLDEIYRSIDDGGDKRSGELKFYRQKVLKKQGK 
Sbjct: 1   MSGQEVRARGERFRDGGGIVQSFSSSLLDEIYRSIDDGGDKRSGELKFYRQKVLKKQGKM 60

Query: 61  IGKAQSDVEDEEIASLHRALLIERWIEKKVAEKVSAQRRRSLTEAEITFQLYQHDREEDV 120
           IGKAQSDVEDEEIASLHRALLIERWIEKKVAEKVSAQRRRSLTEAEI FQLYQ +REEDV
Sbjct: 61  IGKAQSDVEDEEIASLHRALLIERWIEKKVAEKVSAQRRRSLTEAEIKFQLYQQEREEDV 120

Query: 121 LFFSSTSSSSDSSFGGFSSSDTESMYGSKSLTPSCFAKFRPKPVRTSVSAPPPEKIEAKQ 180
           LFFSSTSSSSDSSFGGFSSSDTESMYGSKSLTPSCFAKFRPKPVRTSVSAPPPEK E KQ
Sbjct: 121 LFFSSTSSSSDSSFGGFSSSDTESMYGSKSLTPSCFAKFRPKPVRTSVSAPPPEKTEEKQ 180

Query: 181 RQSREKPQSKQSNQFVETKEKSGGFDDNAMIKSKSRALKIYSNLKKVKQPISPGGRLSSF 240
           RQSREKP +KQ NQFVE  EKS GFD++AMIKSKSRALKIYSNLKKVKQPISPGGRLSSF
Sbjct: 181 RQSREKPPAKQINQFVENNEKSRGFDESAMIKSKSRALKIYSNLKKVKQPISPGGRLSSF 240

Query: 241 LNSIFTAGTPKKTTNSVSSTGVSEDPNSERKSKSGQTSTCSSAASFSRSCLSKSSPCSSE 300
           LNSIFTAGTPKKT NSV+STGVSED NSERKSKSGQTSTCSSAASFSRSCLSK+SPCSSE
Sbjct: 241 LNSIFTAGTPKKTGNSVTSTGVSEDHNSERKSKSGQTSTCSSAASFSRSCLSKNSPCSSE 300

Query: 301 KLRNGDRRTVRFYPVSTIVDEYCRPCGHKSLYEEDPKFQIKHQAETAKSTTTAARKIKTD 360
           KLRN D RTVRFYPVSTIVDEY RPCGHKSL EE PKF++K++AETA++ T A RKI TD
Sbjct: 301 KLRNADTRTVRFYPVSTIVDEYSRPCGHKSLCEEQPKFEVKNKAETAEA-TAAVRKIVTD 360

Query: 361 YQQIRKKNDFLIRNFHHPDNNLYEEEEDDDETASCSSSDLFELDHLRVMSGGDRYREELP 420
           YQQIRKKNDFLIR FHHPDN+LYEEE+++D+TASCSSSDLFELDHL VMS GDRYREELP
Sbjct: 361 YQQIRKKNDFLIRKFHHPDNDLYEEEQEEDDTASCSSSDLFELDHLTVMS-GDRYREELP 419

Query: 421 V 422
           V
Sbjct: 421 V 419

BLAST of Lsi04G013960 vs. ExPASy TrEMBL
Match: A0A5A7VC38 (Protein BIG GRAIN 1-like B OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold134G001850 PE=3 SV=1)

HSP 1 Score: 685.6 bits (1768), Expect = 1.5e-193
Identity = 379/417 (90.89%), Postives = 392/417 (94.00%), Query Frame = 0

Query: 10  GERFRNGG-GNGQSFSSSLLDEIYRSIDDGGDKRSGELKFYRQKVLKKQGKTIGKAQSDV 69
           GERFR+GG GNGQSFSSSLLDEIYRSIDDGGDKRSGELKFYRQKVLKKQGK IGKAQ+DV
Sbjct: 6   GERFRSGGCGNGQSFSSSLLDEIYRSIDDGGDKRSGELKFYRQKVLKKQGKVIGKAQNDV 65

Query: 70  EDEEIASLHRALLIERWIEKKVAEKVSAQRRRSLTEAEITFQLYQHDREEDVLFFSSTSS 129
           EDEEIASLHRALLIERWIEKKVAEKVSAQRRRSLTE EI FQLYQHDREEDVLFFSSTSS
Sbjct: 66  EDEEIASLHRALLIERWIEKKVAEKVSAQRRRSLTEGEIKFQLYQHDREEDVLFFSSTSS 125

Query: 130 SSDSSFGGFSSSDTESMYGSKSLTPSCFAKFRPKPVRTSVSAPPPEKIEAKQRQSREKPQ 189
           SSDSSFGGFSSSDTESMYGSKSLTPSCFAKFRPKP+RTSVSAPPPEK EAKQRQSREKPQ
Sbjct: 126 SSDSSFGGFSSSDTESMYGSKSLTPSCFAKFRPKPIRTSVSAPPPEKTEAKQRQSREKPQ 185

Query: 190 SKQSNQFVETKEKSGGFDDNAMIKSKSRALKIYSNLKKVKQPISPGGRLSSFLNSIFTAG 249
           SKQSNQFVE KEKS GFD+NAMIKSKSRALKIY+NLKKVKQPISPGGRLSSFLNSIFTAG
Sbjct: 186 SKQSNQFVENKEKSRGFDENAMIKSKSRALKIYTNLKKVKQPISPGGRLSSFLNSIFTAG 245

Query: 250 TPKKTTNSVSSTGVSEDPNSERKSKSGQTSTCSSAASFSRSCLSKSSPCSSEKLRNGDRR 309
           TPKKT NSVSST VSEDPNSERKSKSGQTSTCSSAASFSRSCLSK+SPCSSEKLRN DRR
Sbjct: 246 TPKKTGNSVSSTTVSEDPNSERKSKSGQTSTCSSAASFSRSCLSKNSPCSSEKLRNADRR 305

Query: 310 TVRFYPVSTIVDEYCRPCGHKSLY-EEDPKFQIKHQAETAKSTTTAARKIKTDY-QQIRK 369
           TVRFYPVSTIVDEYCRPCGHKSLY EEDPKFQIK++AET +ST   +RK  TDY QQ+RK
Sbjct: 306 TVRFYPVSTIVDEYCRPCGHKSLYEEEDPKFQIKNKAETTEST---SRKNTTDYQQQMRK 365

Query: 370 KNDFLIRNFHHPDNNLYEEEEDDDETASCSSSDLFELDHLRVMSG--GDRYREELPV 422
           KNDFLIRNFHHPDN+L EE   DDETASCSSSDLFELDHLR MSG  G+RYREELPV
Sbjct: 366 KNDFLIRNFHHPDNDLSEE---DDETASCSSSDLFELDHLREMSGGCGNRYREELPV 416

BLAST of Lsi04G013960 vs. ExPASy TrEMBL
Match: A0A1S3BVF5 (protein BIG GRAIN 1-like B OS=Cucumis melo OX=3656 GN=LOC103493690 PE=3 SV=1)

HSP 1 Score: 685.6 bits (1768), Expect = 1.5e-193
Identity = 379/417 (90.89%), Postives = 392/417 (94.00%), Query Frame = 0

Query: 10  GERFRNGG-GNGQSFSSSLLDEIYRSIDDGGDKRSGELKFYRQKVLKKQGKTIGKAQSDV 69
           GERFR+GG GNGQSFSSSLLDEIYRSIDDGGDKRSGELKFYRQKVLKKQGK IGKAQ+DV
Sbjct: 6   GERFRSGGCGNGQSFSSSLLDEIYRSIDDGGDKRSGELKFYRQKVLKKQGKVIGKAQNDV 65

Query: 70  EDEEIASLHRALLIERWIEKKVAEKVSAQRRRSLTEAEITFQLYQHDREEDVLFFSSTSS 129
           EDEEIASLHRALLIERWIEKKVAEKVSAQRRRSLTE EI FQLYQHDREEDVLFFSSTSS
Sbjct: 66  EDEEIASLHRALLIERWIEKKVAEKVSAQRRRSLTEGEIKFQLYQHDREEDVLFFSSTSS 125

Query: 130 SSDSSFGGFSSSDTESMYGSKSLTPSCFAKFRPKPVRTSVSAPPPEKIEAKQRQSREKPQ 189
           SSDSSFGGFSSSDTESMYGSKSLTPSCFAKFRPKP+RTSVSAPPPEK EAKQRQSREKPQ
Sbjct: 126 SSDSSFGGFSSSDTESMYGSKSLTPSCFAKFRPKPIRTSVSAPPPEKTEAKQRQSREKPQ 185

Query: 190 SKQSNQFVETKEKSGGFDDNAMIKSKSRALKIYSNLKKVKQPISPGGRLSSFLNSIFTAG 249
           SKQSNQFVE KEKS GFD+NAMIKSKSRALKIY+NLKKVKQPISPGGRLSSFLNSIFTAG
Sbjct: 186 SKQSNQFVENKEKSRGFDENAMIKSKSRALKIYTNLKKVKQPISPGGRLSSFLNSIFTAG 245

Query: 250 TPKKTTNSVSSTGVSEDPNSERKSKSGQTSTCSSAASFSRSCLSKSSPCSSEKLRNGDRR 309
           TPKKT NSVSST VSEDPNSERKSKSGQTSTCSSAASFSRSCLSK+SPCSSEKLRN DRR
Sbjct: 246 TPKKTGNSVSSTTVSEDPNSERKSKSGQTSTCSSAASFSRSCLSKNSPCSSEKLRNADRR 305

Query: 310 TVRFYPVSTIVDEYCRPCGHKSLY-EEDPKFQIKHQAETAKSTTTAARKIKTDY-QQIRK 369
           TVRFYPVSTIVDEYCRPCGHKSLY EEDPKFQIK++AET +ST   +RK  TDY QQ+RK
Sbjct: 306 TVRFYPVSTIVDEYCRPCGHKSLYEEEDPKFQIKNKAETTEST---SRKNTTDYQQQMRK 365

Query: 370 KNDFLIRNFHHPDNNLYEEEEDDDETASCSSSDLFELDHLRVMSG--GDRYREELPV 422
           KNDFLIRNFHHPDN+L EE   DDETASCSSSDLFELDHLR MSG  G+RYREELPV
Sbjct: 366 KNDFLIRNFHHPDNDLSEE---DDETASCSSSDLFELDHLREMSGGCGNRYREELPV 416

BLAST of Lsi04G013960 vs. ExPASy TrEMBL
Match: A0A6J1IQB0 (protein BIG GRAIN 1-like B OS=Cucurbita maxima OX=3661 GN=LOC111477221 PE=3 SV=1)

HSP 1 Score: 685.3 bits (1767), Expect = 2.0e-193
Identity = 376/423 (88.89%), Postives = 392/423 (92.67%), Query Frame = 0

Query: 1   MSGEEVRARGERFRNGGGNGQSFSSSLLDEIYRSIDDGGDKRSGELKFYRQKVLKKQGKT 60
           MSG+EVR RGERFR+GGG  QSFSSSLLDEIYRSIDDGGDKRSGELKFYRQKVLKKQGK 
Sbjct: 1   MSGQEVRVRGERFRDGGGIVQSFSSSLLDEIYRSIDDGGDKRSGELKFYRQKVLKKQGKM 60

Query: 61  IGKAQSDVEDEEIASLHRALLIERWIEKKVAEKVSAQRRRSLTEAEITFQLYQHDREEDV 120
           IGKAQSDVEDEEIASLHRALLIERWIEKKVAEKVSAQRRRSLTEAEI FQLYQ DREEDV
Sbjct: 61  IGKAQSDVEDEEIASLHRALLIERWIEKKVAEKVSAQRRRSLTEAEIKFQLYQQDREEDV 120

Query: 121 LFFSSTSSSSDSSFGGFSSSDTESMYGSKSLTPSCFAKFRPKPVRTSVSAPPPEKIEAKQ 180
           LFFSSTSSSSDSSFGGFSSSDTESMYGSKSLTPSCFAKFRPKPVRTSVSAPPPEK E KQ
Sbjct: 121 LFFSSTSSSSDSSFGGFSSSDTESMYGSKSLTPSCFAKFRPKPVRTSVSAPPPEKTEEKQ 180

Query: 181 RQSREKPQSKQSNQFVETKEKSGGFDDNAMIKSKSRALKIYSNLKKVKQPISPGGRLSSF 240
           RQSREKP SKQ NQFVE  EKS GFD++AMIKSKSRALKIYSNLKKVKQPISPGGRLSSF
Sbjct: 181 RQSREKPPSKQINQFVENNEKSRGFDESAMIKSKSRALKIYSNLKKVKQPISPGGRLSSF 240

Query: 241 LNSIFTAGTPKKTTNSVSSTGVSEDPNSERKSKSGQTSTCSSAASFSRSCLSKSSPCSSE 300
           LNSIFTAGTPKKT NSV+ST  SED NSERKSKSGQTSTCSSAASFSRSCLSK+SPCSSE
Sbjct: 241 LNSIFTAGTPKKTGNSVTSTRASEDHNSERKSKSGQTSTCSSAASFSRSCLSKNSPCSSE 300

Query: 301 KLRNGDRRTVRFYPVSTIVDEYCRPCGHKSLYEEDPKFQIKHQAETAKSTTTAARKIKTD 360
           KLRN D RTVRFYPVSTIVDEY RPCGHKSL EE+PKF++K++AE A+S T A RKI TD
Sbjct: 301 KLRNADTRTVRFYPVSTIVDEYSRPCGHKSLCEEEPKFEVKNKAEAAES-TAAVRKIVTD 360

Query: 361 YQQIRKKNDFLIRNFHHPDNNLY--EEEEDDDETASCSSSDLFELDHLRVMSGGDRYREE 420
           YQQIRKKNDFLIR FHHPDN+LY  EEEE++D+TASCSSSDLFELDHL VMS GDRYREE
Sbjct: 361 YQQIRKKNDFLIRKFHHPDNDLYEEEEEEEEDDTASCSSSDLFELDHLTVMS-GDRYREE 420

Query: 421 LPV 422
           LPV
Sbjct: 421 LPV 421

BLAST of Lsi04G013960 vs. NCBI nr
Match: XP_038899103.1 (protein BIG GRAIN 1-like A [Benincasa hispida])

HSP 1 Score: 741.5 bits (1913), Expect = 4.9e-210
Identity = 396/421 (94.06%), Postives = 406/421 (96.44%), Query Frame = 0

Query: 1   MSGEEVRARGERFRNGGGNGQSFSSSLLDEIYRSIDDGGDKRSGELKFYRQKVLKKQGKT 60
           MSGEEVR RGERFRNGGGN QSFSSSLLDEIYRSIDDGGDKRSGELKFYRQKVLKKQGKT
Sbjct: 1   MSGEEVRVRGERFRNGGGNVQSFSSSLLDEIYRSIDDGGDKRSGELKFYRQKVLKKQGKT 60

Query: 61  IGKAQSDVEDEEIASLHRALLIERWIEKKVAEKVSAQRRRSLTEAEITFQLYQHDREEDV 120
           IGKA SDVEDEEIASLHRALLIERWIEKKVAEKVSAQRRRSLTEAEI FQLYQHDREEDV
Sbjct: 61  IGKATSDVEDEEIASLHRALLIERWIEKKVAEKVSAQRRRSLTEAEIKFQLYQHDREEDV 120

Query: 121 LFFSSTSSSSDSSFGGFSSSDTESMYGSKSLTPSCFAKFRPKPVRTSVSAPPPEKIEAKQ 180
           LFFSSTSSSSDSSFGGFSSSDTESMYGSKSLTPSCFAKFRPKPVRTSVSAPPPEK EAKQ
Sbjct: 121 LFFSSTSSSSDSSFGGFSSSDTESMYGSKSLTPSCFAKFRPKPVRTSVSAPPPEKTEAKQ 180

Query: 181 RQSREKPQSKQSNQFVETKEKSGGFDDNAMIKSKSRALKIYSNLKKVKQPISPGGRLSSF 240
           RQSREKPQSK SNQFVE KEKSGGFD+NAMIKSKSRALKIYSNLKKVKQPISPGGRLSSF
Sbjct: 181 RQSREKPQSKHSNQFVENKEKSGGFDENAMIKSKSRALKIYSNLKKVKQPISPGGRLSSF 240

Query: 241 LNSIFTAGTPKKTTNSVSSTGVSEDPNSERKSKSGQTSTCSSAASFSRSCLSKSSPCSSE 300
           LNSIFTAGTPKKT NSVSST VSEDPNSERKSKSGQTSTCSSAASFSRSCLSK+SPCSSE
Sbjct: 241 LNSIFTAGTPKKTGNSVSSTSVSEDPNSERKSKSGQTSTCSSAASFSRSCLSKNSPCSSE 300

Query: 301 KLRNGDRRTVRFYPVSTIVDEYCRPCGHKSLYEEDPKFQIKHQAETAKSTTTAARKIKTD 360
           KLRNGDRRTVRFYPVSTIVDEYCRPCGHKSLYEEDPKFQIK++AETA+STT AARKI TD
Sbjct: 301 KLRNGDRRTVRFYPVSTIVDEYCRPCGHKSLYEEDPKFQIKNKAETAESTTAAARKILTD 360

Query: 361 YQQIRKKNDFLIRNFHHPDNNLYEEEEDDDETASCSSSDLFELDHLRVMSGGDRYREELP 420
           YQQ++KKNDFLIRNFHHPDN+L   EED+DETASCSSSDLFELDHL VM+GGDRYREELP
Sbjct: 361 YQQVKKKNDFLIRNFHHPDNDL--SEEDEDETASCSSSDLFELDHLTVMNGGDRYREELP 419

Query: 421 V 422
           V
Sbjct: 421 V 419

BLAST of Lsi04G013960 vs. NCBI nr
Match: XP_011654195.1 (protein BIG GRAIN 1-like B [Cucumis sativus] >KGN55367.1 hypothetical protein Csa_012347 [Cucumis sativus])

HSP 1 Score: 698.7 bits (1802), Expect = 3.7e-197
Identity = 389/427 (91.10%), Postives = 400/427 (93.68%), Query Frame = 0

Query: 1   MSGEEVRAR-GERFRNGG-GNGQSFSSSLLDEIYRSIDDGGDKRSGELKFYRQKVLKKQG 60
           MSGEEVR R GERFRNGG GNGQSFSSSLLDEIYRSIDDGGDKRSGELKFYRQKVLKKQG
Sbjct: 1   MSGEEVRVRSGERFRNGGCGNGQSFSSSLLDEIYRSIDDGGDKRSGELKFYRQKVLKKQG 60

Query: 61  KTIGKAQSDVEDEEIASLHRALLIERWIEKKVAEKVSAQRRRSLTEAEITFQLYQHDREE 120
           K IGKAQSDVEDEEIASLHRALLIERWIEKKVAEKVSAQRRRSLTE EI FQLYQHDREE
Sbjct: 61  KVIGKAQSDVEDEEIASLHRALLIERWIEKKVAEKVSAQRRRSLTEGEIKFQLYQHDREE 120

Query: 121 DVLFFSSTSSSSDSSFGGFSSSDTESMYGSKSLTPSCFAKFRPKPVRTSVSAPPPEKIEA 180
           DVLFFSSTSSSSDSSFGGFSSSDTESMYGSKSLTPSCFAKFRPKP+RTSVSAPPPEK EA
Sbjct: 121 DVLFFSSTSSSSDSSFGGFSSSDTESMYGSKSLTPSCFAKFRPKPIRTSVSAPPPEKTEA 180

Query: 181 KQRQSREKPQSKQSNQFVETKEKSGGFDDNAMIKSKSRALKIYSNLKKVKQPISPGGRLS 240
           KQRQSREKPQSKQSNQFVE KEKS GFD+NAMIKSKSRALKIYSNLKKVKQPISPGGRLS
Sbjct: 181 KQRQSREKPQSKQSNQFVENKEKSRGFDENAMIKSKSRALKIYSNLKKVKQPISPGGRLS 240

Query: 241 SFLNSIFTAGTPKKTTNSVSSTGVSEDPNSERKSKSGQTSTCSSAASFSRSCLSKSSPCS 300
           SFLNSIFTAGTPKKT NSVSST +SEDPNSERKSKSGQTSTCSSA SFSRSCLSKSSPCS
Sbjct: 241 SFLNSIFTAGTPKKTGNSVSSTTLSEDPNSERKSKSGQTSTCSSATSFSRSCLSKSSPCS 300

Query: 301 SEKLRNGDRRTVRFYPVSTIVDEYCRPCGHKSLY-EEDPKFQIKHQAETAKSTTTAARKI 360
           SEKLRN DRRTVRFYPVSTIVDEYCRPCGHKSLY EEDPKFQIK++AE+A+ST   +RK 
Sbjct: 301 SEKLRNADRRTVRFYPVSTIVDEYCRPCGHKSLYEEEDPKFQIKNKAESAEST---SRKN 360

Query: 361 KTDY-QQIRKKNDFLIRNFHHPDNNLYEEEEDDDETASCSSSDLFELDHLRVMSGG--DR 420
            TDY QQ+RKKNDFLIRNFHHPDN+L EE   DDETASCSSSDLFELDHLR MSGG  +R
Sbjct: 361 TTDYQQQMRKKNDFLIRNFHHPDNDLSEE---DDETASCSSSDLFELDHLREMSGGCSNR 420

Query: 421 YREELPV 422
           YREELPV
Sbjct: 421 YREELPV 421

BLAST of Lsi04G013960 vs. NCBI nr
Match: XP_022936713.1 (protein BIG GRAIN 1-like B [Cucurbita moschata])

HSP 1 Score: 692.6 bits (1786), Expect = 2.6e-195
Identity = 376/421 (89.31%), Postives = 395/421 (93.82%), Query Frame = 0

Query: 1   MSGEEVRARGERFRNGGGNGQSFSSSLLDEIYRSIDDGGDKRSGELKFYRQKVLKKQGKT 60
           MSG+EVRARGERFR+GGG  QSFSSSLLDEIYRSIDDGGDKRSGELKFYRQKVLKKQGK 
Sbjct: 1   MSGQEVRARGERFRDGGGIVQSFSSSLLDEIYRSIDDGGDKRSGELKFYRQKVLKKQGKM 60

Query: 61  IGKAQSDVEDEEIASLHRALLIERWIEKKVAEKVSAQRRRSLTEAEITFQLYQHDREEDV 120
           IGKAQSDVEDEEIASLHRALLIERWIEKKVAEKVSAQRRRSLTEAEI FQLYQ +REEDV
Sbjct: 61  IGKAQSDVEDEEIASLHRALLIERWIEKKVAEKVSAQRRRSLTEAEIKFQLYQQEREEDV 120

Query: 121 LFFSSTSSSSDSSFGGFSSSDTESMYGSKSLTPSCFAKFRPKPVRTSVSAPPPEKIEAKQ 180
           LFFSSTSSSSDSSFGGFSSSDTESMYGSKSLTPSCFAKFRPKPVRTSVSAPPPEK E KQ
Sbjct: 121 LFFSSTSSSSDSSFGGFSSSDTESMYGSKSLTPSCFAKFRPKPVRTSVSAPPPEKTEEKQ 180

Query: 181 RQSREKPQSKQSNQFVETKEKSGGFDDNAMIKSKSRALKIYSNLKKVKQPISPGGRLSSF 240
           RQSREKP +KQ NQFVE  EKS GFD++AMIKSKSRALKIYSNLKKVKQPISPGGRLSSF
Sbjct: 181 RQSREKPPAKQINQFVENNEKSRGFDESAMIKSKSRALKIYSNLKKVKQPISPGGRLSSF 240

Query: 241 LNSIFTAGTPKKTTNSVSSTGVSEDPNSERKSKSGQTSTCSSAASFSRSCLSKSSPCSSE 300
           LNSIFTAGTPKKT NSV+STGVSED NSERKSKSGQTSTCSSAASFSRSCLSK+SPCSSE
Sbjct: 241 LNSIFTAGTPKKTGNSVTSTGVSEDHNSERKSKSGQTSTCSSAASFSRSCLSKNSPCSSE 300

Query: 301 KLRNGDRRTVRFYPVSTIVDEYCRPCGHKSLYEEDPKFQIKHQAETAKSTTTAARKIKTD 360
           KLRN D RTVRFYPVSTIVDEY RPCGHKSL EE PKF++K++AETA++ T A RKI TD
Sbjct: 301 KLRNADTRTVRFYPVSTIVDEYSRPCGHKSLCEEQPKFEVKNKAETAEA-TAAVRKIVTD 360

Query: 361 YQQIRKKNDFLIRNFHHPDNNLYEEEEDDDETASCSSSDLFELDHLRVMSGGDRYREELP 420
           YQQIRKKNDFLIR FHHPDN+LYEEE+++D+TASCSSSDLFELDHL VMS GDRYREELP
Sbjct: 361 YQQIRKKNDFLIRKFHHPDNDLYEEEQEEDDTASCSSSDLFELDHLTVMS-GDRYREELP 419

Query: 421 V 422
           V
Sbjct: 421 V 419

BLAST of Lsi04G013960 vs. NCBI nr
Match: XP_008452771.1 (PREDICTED: protein BIG GRAIN 1-like B [Cucumis melo] >KAA0064527.1 protein BIG GRAIN 1-like B [Cucumis melo var. makuwa] >TYK20063.1 protein BIG GRAIN 1-like B [Cucumis melo var. makuwa])

HSP 1 Score: 685.6 bits (1768), Expect = 3.2e-193
Identity = 379/417 (90.89%), Postives = 392/417 (94.00%), Query Frame = 0

Query: 10  GERFRNGG-GNGQSFSSSLLDEIYRSIDDGGDKRSGELKFYRQKVLKKQGKTIGKAQSDV 69
           GERFR+GG GNGQSFSSSLLDEIYRSIDDGGDKRSGELKFYRQKVLKKQGK IGKAQ+DV
Sbjct: 6   GERFRSGGCGNGQSFSSSLLDEIYRSIDDGGDKRSGELKFYRQKVLKKQGKVIGKAQNDV 65

Query: 70  EDEEIASLHRALLIERWIEKKVAEKVSAQRRRSLTEAEITFQLYQHDREEDVLFFSSTSS 129
           EDEEIASLHRALLIERWIEKKVAEKVSAQRRRSLTE EI FQLYQHDREEDVLFFSSTSS
Sbjct: 66  EDEEIASLHRALLIERWIEKKVAEKVSAQRRRSLTEGEIKFQLYQHDREEDVLFFSSTSS 125

Query: 130 SSDSSFGGFSSSDTESMYGSKSLTPSCFAKFRPKPVRTSVSAPPPEKIEAKQRQSREKPQ 189
           SSDSSFGGFSSSDTESMYGSKSLTPSCFAKFRPKP+RTSVSAPPPEK EAKQRQSREKPQ
Sbjct: 126 SSDSSFGGFSSSDTESMYGSKSLTPSCFAKFRPKPIRTSVSAPPPEKTEAKQRQSREKPQ 185

Query: 190 SKQSNQFVETKEKSGGFDDNAMIKSKSRALKIYSNLKKVKQPISPGGRLSSFLNSIFTAG 249
           SKQSNQFVE KEKS GFD+NAMIKSKSRALKIY+NLKKVKQPISPGGRLSSFLNSIFTAG
Sbjct: 186 SKQSNQFVENKEKSRGFDENAMIKSKSRALKIYTNLKKVKQPISPGGRLSSFLNSIFTAG 245

Query: 250 TPKKTTNSVSSTGVSEDPNSERKSKSGQTSTCSSAASFSRSCLSKSSPCSSEKLRNGDRR 309
           TPKKT NSVSST VSEDPNSERKSKSGQTSTCSSAASFSRSCLSK+SPCSSEKLRN DRR
Sbjct: 246 TPKKTGNSVSSTTVSEDPNSERKSKSGQTSTCSSAASFSRSCLSKNSPCSSEKLRNADRR 305

Query: 310 TVRFYPVSTIVDEYCRPCGHKSLY-EEDPKFQIKHQAETAKSTTTAARKIKTDY-QQIRK 369
           TVRFYPVSTIVDEYCRPCGHKSLY EEDPKFQIK++AET +ST   +RK  TDY QQ+RK
Sbjct: 306 TVRFYPVSTIVDEYCRPCGHKSLYEEEDPKFQIKNKAETTEST---SRKNTTDYQQQMRK 365

Query: 370 KNDFLIRNFHHPDNNLYEEEEDDDETASCSSSDLFELDHLRVMSG--GDRYREELPV 422
           KNDFLIRNFHHPDN+L EE   DDETASCSSSDLFELDHLR MSG  G+RYREELPV
Sbjct: 366 KNDFLIRNFHHPDNDLSEE---DDETASCSSSDLFELDHLREMSGGCGNRYREELPV 416

BLAST of Lsi04G013960 vs. NCBI nr
Match: XP_022977029.1 (protein BIG GRAIN 1-like B [Cucurbita maxima])

HSP 1 Score: 685.3 bits (1767), Expect = 4.2e-193
Identity = 376/423 (88.89%), Postives = 392/423 (92.67%), Query Frame = 0

Query: 1   MSGEEVRARGERFRNGGGNGQSFSSSLLDEIYRSIDDGGDKRSGELKFYRQKVLKKQGKT 60
           MSG+EVR RGERFR+GGG  QSFSSSLLDEIYRSIDDGGDKRSGELKFYRQKVLKKQGK 
Sbjct: 1   MSGQEVRVRGERFRDGGGIVQSFSSSLLDEIYRSIDDGGDKRSGELKFYRQKVLKKQGKM 60

Query: 61  IGKAQSDVEDEEIASLHRALLIERWIEKKVAEKVSAQRRRSLTEAEITFQLYQHDREEDV 120
           IGKAQSDVEDEEIASLHRALLIERWIEKKVAEKVSAQRRRSLTEAEI FQLYQ DREEDV
Sbjct: 61  IGKAQSDVEDEEIASLHRALLIERWIEKKVAEKVSAQRRRSLTEAEIKFQLYQQDREEDV 120

Query: 121 LFFSSTSSSSDSSFGGFSSSDTESMYGSKSLTPSCFAKFRPKPVRTSVSAPPPEKIEAKQ 180
           LFFSSTSSSSDSSFGGFSSSDTESMYGSKSLTPSCFAKFRPKPVRTSVSAPPPEK E KQ
Sbjct: 121 LFFSSTSSSSDSSFGGFSSSDTESMYGSKSLTPSCFAKFRPKPVRTSVSAPPPEKTEEKQ 180

Query: 181 RQSREKPQSKQSNQFVETKEKSGGFDDNAMIKSKSRALKIYSNLKKVKQPISPGGRLSSF 240
           RQSREKP SKQ NQFVE  EKS GFD++AMIKSKSRALKIYSNLKKVKQPISPGGRLSSF
Sbjct: 181 RQSREKPPSKQINQFVENNEKSRGFDESAMIKSKSRALKIYSNLKKVKQPISPGGRLSSF 240

Query: 241 LNSIFTAGTPKKTTNSVSSTGVSEDPNSERKSKSGQTSTCSSAASFSRSCLSKSSPCSSE 300
           LNSIFTAGTPKKT NSV+ST  SED NSERKSKSGQTSTCSSAASFSRSCLSK+SPCSSE
Sbjct: 241 LNSIFTAGTPKKTGNSVTSTRASEDHNSERKSKSGQTSTCSSAASFSRSCLSKNSPCSSE 300

Query: 301 KLRNGDRRTVRFYPVSTIVDEYCRPCGHKSLYEEDPKFQIKHQAETAKSTTTAARKIKTD 360
           KLRN D RTVRFYPVSTIVDEY RPCGHKSL EE+PKF++K++AE A+S T A RKI TD
Sbjct: 301 KLRNADTRTVRFYPVSTIVDEYSRPCGHKSLCEEEPKFEVKNKAEAAES-TAAVRKIVTD 360

Query: 361 YQQIRKKNDFLIRNFHHPDNNLY--EEEEDDDETASCSSSDLFELDHLRVMSGGDRYREE 420
           YQQIRKKNDFLIR FHHPDN+LY  EEEE++D+TASCSSSDLFELDHL VMS GDRYREE
Sbjct: 361 YQQIRKKNDFLIRKFHHPDNDLYEEEEEEEEDDTASCSSSDLFELDHLTVMS-GDRYREE 420

Query: 421 LPV 422
           LPV
Sbjct: 421 LPV 421

BLAST of Lsi04G013960 vs. TAIR 10
Match: AT1G54200.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT3G13980.1); Has 1084 Blast hits to 581 proteins in 136 species: Archae - 0; Bacteria - 72; Metazoa - 212; Fungi - 78; Plants - 102; Viruses - 0; Other Eukaryotes - 620 (source: NCBI BLink). )

HSP 1 Score: 162.9 bits (411), Expect = 6.7e-40
Identity = 159/409 (38.88%), Postives = 210/409 (51.34%), Query Frame = 0

Query: 22  SFSSSLLDEIYRSIDDGGDKRSGELKFYRQKVLKKQGKTIGKAQSDVEDEEIASLHRALL 81
           SFSS+LLD+IYRSIDD     S   K   Q                              
Sbjct: 27  SFSSTLLDQIYRSIDDSSTNSSSMRKTKHQ------------------------------ 86

Query: 82  IERWIEKKVAEKVSAQRRRSLTEAEITFQLYQHDREEDVLFFSSTSSSSDSSFGGFSSSD 141
                  +   +VSA RR     ++    +      E V F  S+SSSSDSS  GFSSS+
Sbjct: 87  ------NREDTRVSANRRDDFNRSKNLKTI------EPVFFKHSSSSSSDSS--GFSSSE 146

Query: 142 TESMYGSKSLTPSCFAKFRPKPVRTSVSAPPPEKIEAKQRQSREKPQSKQSNQFVETKEK 201
           ++  Y     +P   A   PKP+RT+V     E+ E         PQ+ + N     K++
Sbjct: 147 SDYFYRRSKSSP---AISHPKPIRTTV-----ERFE-------RSPQNHRPNS--SNKQE 206

Query: 202 SGGFDDNAMIKSKSRALKIYSNLKKVKQPISPGGRLSSFLNSIFTAGTPKKTTNSVSSTG 261
            G F     +K+KS+ALKIYS+LKKVKQPISPGGRL++FLNSIFT     K  N +++T 
Sbjct: 207 HGSF-----LKTKSKALKIYSDLKKVKQPISPGGRLATFLNSIFTGAGNTKKLNKINTTV 266

Query: 262 VSEDPNSERKSKSGQTSTCSSAASFSRSCLSKSSPCSSEKLRNGDRRTVRFYPVSTIVDE 321
            S    +   + +  T+TCSSA+SFSRSCLSK +P SSEK     +R+VRF PV+ I DE
Sbjct: 267 TS---TTAAAAAASSTTTCSSASSFSRSCLSK-TPSSSEK----SKRSVRFCPVNVIFDE 326

Query: 322 YCRPCGHKS--LY--EEDPKFQIKHQAETA-----KSTTTAARKIKTDYQQIRKKNDFLI 381
                 +K+  +Y   E     I+H  E       +    AA+++   YQ   KKN  +I
Sbjct: 327 DSSKYNNKNNKVYGNNEREYESIRHTLENRVMEENRRVIEAAKELLRSYQ---KKNKEVI 352

Query: 382 RNFHHPDNNLYEEEEDDDETASCSSSDLFELDHLRVMSGGDRYREELPV 422
                 D     EE+DDD+  SC+SSDLFELD+L  + G DRYREELPV
Sbjct: 387 EVSVEDD-----EEDDDDDALSCTSSDLFELDNLSAI-GIDRYREELPV 352

BLAST of Lsi04G013960 vs. TAIR 10
Match: AT3G13980.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G54200.1); Has 1485 Blast hits to 418 proteins in 98 species: Archae - 0; Bacteria - 6; Metazoa - 246; Fungi - 61; Plants - 107; Viruses - 6; Other Eukaryotes - 1059 (source: NCBI BLink). )

HSP 1 Score: 159.5 bits (402), Expect = 7.4e-39
Identity = 149/410 (36.34%), Postives = 215/410 (52.44%), Query Frame = 0

Query: 22  SFSSSLLDEIYRSIDDGGDKRSGELKFYRQKVLKKQGKTIGKAQSDVEDEEIASLHRALL 81
           SFSS+LLD+IYRSIDD       E       + KK+     +  S  ED EI+ ++    
Sbjct: 18  SFSSTLLDQIYRSIDDSSPPPPLE------SIKKKKHHHQQRNASLHEDREISPIY---- 77

Query: 82  IERWIEKKVAEKVSAQRRRSLTEAEITFQLYQHDREEDVLFFSSTSSSSDSSFGGFSSSD 141
                            RRS+         ++  R +      S SSSSDSS  GFSSS+
Sbjct: 78  ----------------HRRSIAAD------FERSRRKTDFLRHSNSSSSDSS--GFSSSE 137

Query: 142 TESMYG--SKSLTPSCFAKFRPKPVRTSVSAPPPEKIEAKQRQSREKPQSKQSNQFVETK 201
           ++S +G    S +P   ++ +PKP+RTS                     S   +  V+  
Sbjct: 138 SDSFHGRSKSSASPPSSSRQQPKPIRTS---------------------SVDHSSAVQKP 197

Query: 202 EKSGGFDDNAMIKSKSRALKIYSNLKKVKQPISPGGRLSSFLNSIFT-AGTPKKTTNSVS 261
           ++ GGF     +++KS+ALKIYS+LKKVKQPISPGGRL++FLNS+FT A T  K     +
Sbjct: 198 KELGGF-----LRTKSKALKIYSDLKKVKQPISPGGRLATFLNSLFTNAATNPKKHKKTT 257

Query: 262 STGVSEDPNSERKSKSGQTSTCSSAASFSRSCLSKSSPCSSEKLRNGDRRTVRFYPVSTI 321
           +  V E+P+S        +STCSSA+SFSRSCLSK +P SS K     +R+VRF PV+ I
Sbjct: 258 TVAVVEEPHS--------SSTCSSASSFSRSCLSK-TPSSSGK----SKRSVRFCPVNVI 317

Query: 322 VDEYCRPCGHKSLYEEDPKFQIKHQAETAKSTTTAARKIKTDYQQIRKKNDFLIRNFHHP 381
           +D             ED  F + +     +       K   +++++ +    L+R +H+ 
Sbjct: 318 LD-------------EDSSFTMPYAYNNERLYDNNEAKRVEEHRRVIQAAKDLLRTYHNK 338

Query: 382 D-------NNLYEEEEDDDETASCSSSDLFELDHLRVMSGGDRYREELPV 422
           +       NN+  EE+D+D+ ASC+SSDLFEL++L  + G +RYREELPV
Sbjct: 378 NKVTTTNINNV--EEDDEDDAASCASSDLFELENLSAI-GIERYREELPV 338

BLAST of Lsi04G013960 vs. TAIR 10
Match: AT5G12050.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT3G13980.1); Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink). )

HSP 1 Score: 99.8 bits (247), Expect = 7.0e-21
Identity = 109/334 (32.63%), Postives = 161/334 (48.20%), Query Frame = 0

Query: 98  RRRSLTEAEITFQLYQHDREEDVLFFSSTSSSSDSSFGGFSSSDTESMYGSK--SLTPSC 157
           +++S+    +  +    DR     FF S SSSSDS+   FSSSDTE  +G K  S  P C
Sbjct: 41  KKQSIVTRSVPDRKIHRDR-----FFGSVSSSSDSNSSIFSSSDTELTHGKKTTSSRPLC 100

Query: 158 FAKFRPKPVRTSVSAPPPEKIEAKQRQSREKPQSKQSNQFVETKEKSGGFDDNAMIKSKS 217
           F   + KP +T       +K    Q ++    +      +     K    D+N       
Sbjct: 101 FGPSKTKPRKTE------DKTLFHQNRA---TRVYDDYDYASDVPKFNRHDENWENTRNR 160

Query: 218 RALKIYSNLKKVKQPISPGGRLSSFLNSIFTAGTPKKTTNSVSS----TGVSEDPNSERK 277
           R++K   N KK K P SPGGR+ +FLNS+F+  +  K +N+V S    T   +     + 
Sbjct: 161 RSVKSSGNQKKPKTPASPGGRIVNFLNSLFSNNS--KQSNAVKSYPRKTSYDDSAYVRKT 220

Query: 278 SKS--GQTSTCSSAASFSRSCLSKSSPCSSEKLRNGDRRTVRFYPVSTIVDEYCRPCGHK 337
           S      T+TCSSA+SFSRSC++K    SS ++    +R+VRF PV+ IV E        
Sbjct: 221 SNDYHSSTTTCSSASSFSRSCMNKGYEKSSGRI----KRSVRFSPVNVIVPESFTSKEED 280

Query: 338 SLYEEDPKFQIKHQAETA--KSTTTAARKIKTDYQQIRKKNDFLIRNFHHPDNNLYEEEE 397
                + +  +K   E    +S    AR+   DY   +   + L++     D    +E++
Sbjct: 281 YFSNGNARKSVKKNVEDGGRRSVEEIAREFLRDYH--KNHENSLVKTNGFEDYEDDDEDD 340

Query: 398 DDDETASCSSSDLFELDHLRVMSGGDRYREELPV 422
           DDD+ AS SSSDLFELD +      + Y +ELPV
Sbjct: 341 DDDDVASDSSSDLFELDLVGNHHHHNVYGDELPV 352

BLAST of Lsi04G013960 vs. TAIR 10
Match: AT3G42800.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G54200.1); Has 853 Blast hits to 340 proteins in 98 species: Archae - 0; Bacteria - 94; Metazoa - 145; Fungi - 62; Plants - 95; Viruses - 0; Other Eukaryotes - 457 (source: NCBI BLink). )

HSP 1 Score: 84.7 bits (208), Expect = 2.3e-16
Identity = 113/410 (27.56%), Postives = 178/410 (43.41%), Query Frame = 0

Query: 22  SFSSSLLDEIYRSIDDGGDKRSGELKFYRQKVLKKQGKTIGKAQSDVEDEEIASLHRALL 81
           SFSSS+LD +YRSID+  D    +LK     + +    +      + +D+++ +L RA++
Sbjct: 11  SFSSSVLDSVYRSIDE-SDGLQSDLK---GSINENVSSSSSSPSPNKKDDKLTTLRRAIM 70

Query: 82  IERWIEKKVAEKVSAQRRRSLTEAEITFQLYQHDREEDVLFFSSTSSSSDSSFGGFSSSD 141
                                            D E  +   SST++++ S    FSSS+
Sbjct: 71  ---------------------------------DEEHWLYARSSTTTTNSSDSSSFSSSE 130

Query: 142 TESMYGSKSLTPSCFAKFRPKPVRTSVSAPPPEKIEAKQRQSREKPQSKQSNQFVETKEK 201
            ES    + L                      +  E  +R   E+ ++K++    +++  
Sbjct: 131 AESYRTKRRLR---------------------KLAEQGKRSGDERQRTKRTVMDNDSRLF 190

Query: 202 SGGFDDNAMIKSKSRALKIYSNLKKVKQPISPGGRLSSFLNSIFTAGTPKKTTNSVSSTG 261
           S   DD      K +A+KI   LK+ KQP+SPG RL+SFLNSIF +   K    SV  T 
Sbjct: 191 SKSDDD-----KKPKAVKIIEELKRSKQPVSPGARLTSFLNSIFQSNAKKVKLCSVGKT- 250

Query: 262 VSEDPNSERKSKSGQTSTCSSAASFSRSCLSKSSPCSSEKLRNGDR--RTVRFYPVSTIV 321
                 ++ KS S            S+SC S++   +     N  +  R++RFYPV   +
Sbjct: 251 ------TDVKSSS------------SKSCFSRTRNKTDNNNNNCKKLERSIRFYPVRVTI 310

Query: 322 DEYCRPCGHKSL---YEEDPKFQIKHQAETAKSTTTAARKIKTDYQQIRKKNDF--LIRN 381
           D  CR    K +    +  P+F             TA + +K + +      +F  + RN
Sbjct: 311 DGDCRDYAQKHITRVRKPIPEF-------------TAKKSVKEEIKTNDHHTEFTCITRN 323

Query: 382 FHHPD---NNLYEEEEDDDETASCSSSDLFELDHLRVMSGGDRYREELPV 422
               D   +N YE +E++++  S SSSDLFELD  R+  G  RY +ELPV
Sbjct: 371 IGLKDFVRSNKYEGKEEEEDAWSHSSSDLFELDSYRI--GMGRYLKELPV 323

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SLL29.5e-3938.88Protein BIG GRAIN 1-like B OS=Arabidopsis thaliana OX=3702 GN=At1g54200 PE=1 SV=... [more]
Q9LVK21.0e-3736.34Protein BIG GRAIN 1-like A OS=Arabidopsis thaliana OX=3702 GN=At3g13980 PE=3 SV=... [more]
Q9LYH09.8e-2032.63Protein BIG GRAIN 1-like D OS=Arabidopsis thaliana OX=3702 GN=At5g12050 PE=2 SV=... [more]
Q9M2B33.3e-1527.56Protein BIG GRAIN 1-like C OS=Arabidopsis thaliana OX=3702 GN=At3g42800 PE=2 SV=... [more]
Match NameE-valueIdentityDescription
A0A0A0L4381.8e-19791.10Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G646410 PE=3 SV=1[more]
A0A6J1FE051.3e-19589.31protein BIG GRAIN 1-like B OS=Cucurbita moschata OX=3662 GN=LOC111443225 PE=3 SV... [more]
A0A5A7VC381.5e-19390.89Protein BIG GRAIN 1-like B OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaff... [more]
A0A1S3BVF51.5e-19390.89protein BIG GRAIN 1-like B OS=Cucumis melo OX=3656 GN=LOC103493690 PE=3 SV=1[more]
A0A6J1IQB02.0e-19388.89protein BIG GRAIN 1-like B OS=Cucurbita maxima OX=3661 GN=LOC111477221 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_038899103.14.9e-21094.06protein BIG GRAIN 1-like A [Benincasa hispida][more]
XP_011654195.13.7e-19791.10protein BIG GRAIN 1-like B [Cucumis sativus] >KGN55367.1 hypothetical protein Cs... [more]
XP_022936713.12.6e-19589.31protein BIG GRAIN 1-like B [Cucurbita moschata][more]
XP_008452771.13.2e-19390.89PREDICTED: protein BIG GRAIN 1-like B [Cucumis melo] >KAA0064527.1 protein BIG G... [more]
XP_022977029.14.2e-19388.89protein BIG GRAIN 1-like B [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
AT1G54200.16.7e-4038.88unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TA... [more]
AT3G13980.17.4e-3936.34unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TA... [more]
AT5G12050.17.0e-2132.63unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TA... [more]
AT3G42800.12.3e-1627.56unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TA... [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..22
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 251..265
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 251..281
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 162..209
NoneNo IPR availablePANTHERPTHR33541:SF12PROTEIN BIG GRAIN 1-LIKE Bcoord: 4..421
IPR039621Protein BIG GRAIN 1-likePANTHERPTHR33541PROTEIN BIG GRAIN 1-LIKE A-RELATEDcoord: 4..421

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi04G013960.1Lsi04G013960.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009734 auxin-activated signaling pathway
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005886 plasma membrane