Lsi04G012100 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi04G012100
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	Cytochrome P450
Location	chr04: 18669643 .. 18676529 (+)
RNA-Seq Expression	Lsi04G012100
Synteny	Lsi04G012100

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGATAACCATAATCCTTCTTCTCATCACCATCTTCCTCTTCCTCAGATTTAGATCAACATTCTCCTCCTCCGAGGCCGCGAAAAACCTTCCTCCGGGGTCACTTGGGGTTCCATTTATAGGCCAAAGCCTGAGCCTTCTTCGAGCTATGCGAACAAACTCAGCAGAGCAATGGCTGCAGAAGAGAGTGGCCAAATACGGGCCAGTTTCGAAGATGACGCTGTTCGGGAAGCCGACGGTGTTTGTAAATGGGGCAGCGGCGAATAAGGCGGTGGTGTTTTCGGGGGAGGAGGGGACGGTGTCGAGCCGGCAGATAGAGTCGTTAAAGAGGATTTTGGGAGAGAGGAATTTGACGGAGCTGAGTGGTGAGGATCATAAGAGAGTTAGAGGGGCGCTGGTTTGGTTCTTGAGGCCTCAGAGCTTGAAAGGCTTTGTGGGGAAAATGGATGGAGAAGTTAGGAAGCATTTGAACATGTATTGGCATGGCAACAAAGAAGTCACCGTGAGTTCTCTAACTTTTTTTTAGTTAAAATACTATTTTGGTCTTTATATCTTGATGTTTATTCAATTTTAGTATCCGTATTTTCCGTTGTTCATTTTATTAATTACTGTATTTTCAATTAATCTTAAATTTAGTCTTGACTACTAATTTATTGTTGATTTTTTTTATCTATTAGCATTTTTACCATACATTTTAAAAACATATTCACCTATTGTACCTCTTTGAAGGGAAAAGTATTAGAATCATTTAATCAATTTCGATAAAAATTAACTTTGAGCAATTGAATTTAAAAAAATTTTAAATACAGAGACTAAAATTAGACAATTAAAAATACAGAAACGAAAATTAAACAAAGCTTCAAGATATAGCGACAAAAAAATGGTATTTTAACAGTTTTTTTCCTTCTTTTGTAAAAAAAAAAAAGTGTTAAAACTACAAGTTTAGTCTCTAATTTTTTATATCTCTCATAAGTCACTTGACTTTAAAGCATATTTAATAAGTCTTTAAACTTTTAATTTGGCATCTAGTAGGATTATTGAACTTTAAAAAGTCATTTATTATACACCAAATTAAAACTCAAGTCTATTAGACTTAAATTGAAATTTTACTTATTTAGATTGGTGAACTTCTATAAATTAACGTTGTTTTTTCTAAAACTTAGTGGTGACAACAAAAACTTCTACTATTTAATTCTCATAAAACTAAGCAATTGCCAATTCAAACCTAACTTAATCATTAAAAGACATGAATTGTTTTCTCTAAAATATAATCCAAGTTTTGATATTCATAGTGTCCTATATTTGTTTGACATAAAAAACCTACAAACACACTTAATAAAACATAAAGTGGATACATAATAATTCTCCAAACTTCATGGTTTAGATTTTTGAAAACATTTTGTAGGGAGTAGACTCGTATTCGAGTGCGTTTGAGATTGATTTTGGAAAAGCGAATGGAAGTAATTATAAAAGAATCTTAATCATTTATGTGTTTTGGAAAACAAATTATAATTAAATTTATTAAAATCACCTCTAAATCACATGTTCATTTTACATCCTTTTAAAATCGGTTCTTTATTAGTTTTTAGGAGAGTATATAAAATTTTTAATAGATTTTTAATTTTAATCAGCTCCTTGAAAATTAAGATTTATTTTATATATCTATTTTAAACAATTATTTAATAATTATAAGAATAATTTTTAAAAATAGAAAAATAAGAGAAATTATTTACACATTATTTACACAAAATAGTAAAACTTTCAGATAGTTGTGATATACGCTGATAGAAGTCTATCAGGGTCTATCAGTGATAGAAATGATAGAAGTCTATCACTGTTAGATACTGATAAAAGTTTATCAATATCTATCAGTGTTTTTTTGCTATTTTATATAAATAGTTTGATATTTTTTATATGTGAAAATATGACTAATTATAAATTATAAAAATAATATTAAGTCAGTTTTAGTTTTCCTATATAAAACATGATAATTTAGTATTTATTTACCAAATACCTTTTACCAACATGTTCAATTATAAAGTTGAAATTTACCAAATGCTAATACGCTTCATATTACAGTTAATTTTACAAAAAAATATAGTTTATTTTAAATGATATAGTAATCCTAAACGAAACCTTCATAAGCTTAATTTTAAAAAAATTAAAACCAACAGCTTATAACCCATTTTAATAACTGGTCTTTTTATTTTAGTTTTTGATAATTGTGTTTCTTTTCTTAAAAAAAAATTTACAATAATTTTCATATTTCTTAAGGAATTGCGGAATGTATATTTTTATTCACTCACTCAATCACTAGCTATAAATATCAATTCTACATGCTATAAATAGCTTAATTGACAACATAAAATGAGGAATAAAATCGATTAATCTAACTTCAAAAAACTAGCCACTAGCTAATATAATCCTTAGCCAAATTCAAAAAACAAAAACGACTTTTTAGTAAGGTAATTGTAATGAGTAGCAATTTTAGGAATAATAATCAAGTGTATAGCAACATTTTAAAAAATTGTAAATATAACAAAGTCTATCAGTGATAAAATTCTATCGCTGATGGACTATTTGAAGGTTTGAATTCCCTGCAGAAGCGATTTATCGCATTGTGGTTTCAATTACTCAATTATGAAACTTAAAGAAACAGAAAAAAAATATATAACAGGATAAACAACATGATTTTGATATGAAATGTAAAAATTAAACTTTATATTATAATGTATCAATATACATTATTTAATGGAAAATTTTTAAAAATATAAAAATAAGAGAATCTATTTACACAAAATAGCAAAATTTTTAAATAGTTGTGATATATGTTGATAGAAGTCTATCAGGGTCTATCAGTAATAGAAATCATAGAAGTCTTATCACTATAGATGCTGATAGAAGTCTATCAATGTCTATCAATGTTTTTTTTTTTGTTATTTTCTATAAATAGTTTGGCATTTTTTCTATCTGTGAAAATTTTTCTTATTTAATTGTATCTCATACAATTAATTCCCTTAACTTAATTTGAACAATTCAAATAAAACCAAGACAGCTGAGCGCCATCTTAACCTATATGAGCTAGCAAGTTGACCTAATGGACCTATCAGTTTGAAGCTCTAATGGTACGAGATTAATTAATTAAACTCTTTAATTAAACTAATCAACATTCCACTAAAGACCGACAGCTGCACTCTTCTTACTGTAGATATATTTCTATATCCACGGATATAATCAATCAACAACGAGTCGACCCTTCACAAATTGCTCGTAACAACAACTAGGTCAAATTACCGTTTTACCCCTGTGTTACCTCTAACTCCTTAAGTACCACAGATCCCTTTAATGAACAATTTAGTCATAGTCCAACTATGAGCTAGACCCCTCTCTGGCCAGTGAGAGGGTGAGGCGCCTCATTGTTCAAGACCCGACATCAGCTCTTAAGGGAGCAATTCATAAGACATGAATTCCTTATTGTACAGTTGAGTTTCCAGCTGCCTGCTCGGACAAATCCCCAAAGTGGTAGGCTTATTGAGTCGGCGAACCTAGCCACTCTCACCCATACAAATTAAAGGATTGCCCTCACAGAAAGAAATTCACAACTGACTTAGGATTAAGGTCGAGTCACCTATGATTATCCTCGTGAAATGTAAATCTCATTAAGTAACGGTGTTAATACGGGAGAATATTCATTTTGCGGTCTGGTCTTATATATGTCTCATATATATAGGATACCCCCACTCACATGTCTCCACATGAACATTTAAGATCAAACGTTTGTGACACTTTACAACACTTGTAACAATCACAAAGTGGGGCATATCCGTAGTGTTACCAGAATAAGGTATCCAACCTTATCCATCTACTACAAACCGTTTAGGTTATCACTTAAACACGATCCACTTGTATGTCTCTACATACGTGTTTAAGTTACTACTAATAATCTAGGATCTTAGTTTATTGGTTTTTGGGTTAGTGCAACTAAATGTCAAAGAAAATAACAATTATTTTATTAAATAAATAATTTGGTTTGTACAATACAAGTTTACAAAATACAAAACCACGGGATTTAGGGCATTAACCCCAACACTATTATGGTCTATCAGTGATAGACCACATAGACCATAAAAGTCTCTAATAGCGATAGAAGTCTTTCACTGATAGACTTTGTTATATTTGTAATTTTTTAAAAATGTTGCTATATATGCTAATACTTTGAATCGGATTGCTATATTTGCAACTGTCCTTTTCTAAAAACTACTTTTTCTAAATCGCCATTAAACAGAGTTTTAACTAACTTTTGCGAAAACACACAGGTGTCACCATTGATGAAAACTCTAACATTTGACATCATATGCTCTCTCCTGTTTGGCATTGAAGAAGGACCAACAAGAAAAAGCATAATAGAATGCTTCAAAACAATGGTGGATGGCATTTGGTCTGTTCCAATCAACTTGCCATTCACAAGGTATAGCCACAGCCTCAAAGCAAGTGCAAAAGCTCAACAGATATTGAAGCAACTTTTGAAAGACAAAACACAGACAATGGAAGAAGAACAACAAGAAGATGATGATAAAGACCTAATTACTTATCTGTTTAGCATCAAGAACAAAGACAAAGAACAAGCTTTGAGTGAGGAGGAAATTGTGCACAATATCATCCTTCTAATGATTGCTGGACATGACACAACATCCATTTTGATTACACTTATGCTTAGGGTTTTGGCTAGAAACCCAACTGTTTATGCAGCTGTTCTTCAAGGTATGAATGAATAAATTTCTATTTCAATCTTAATGGGAGGTCGATTGACACGTTCAATGATTTCAGAAAAACGACGTTATATTTTATTAAAATTGGGTTGTGGTATAAGATTTTTTGAGTTATTAGAGTTTAAAGTGAGTTGTTACGAATGTCTTGACATGCAAATTCGAAAAAACAGATATCATATTGTCGAGTTGAGTTGAATTGGATTCATTTATTAGAAAGTTAATTAAAATTCATCTTCGTTTCGAGTCAAAGGTGAGCTTAACTTAACTATAATTGACATGTACTTTCTCCCTTGAAGTCTTTTGAGGTCAGTCTTTTGAGGTGGGTAATTCGATTCCCTATTGCACTAAAATATAAACTATTTATGATTTTAGAGCATGAAGAAATAGCAAGAAACAAAGAAAGTGGGGAGGCACTCAGTTGGGAAGATGTTTCCAAGATGAAGTACACATGGAAAGTGGCAATGGAAACCTTGAGACTTTACCCACCTGTATTTGGAGGGTTTAGAGTGGCCCTTAAAGATATTCAATTAGGTGGATACACAATTCCAAAAGGATGGCAAGTAAGCGAAGTTTTTCCTTTCTTTCTTTTTAACTATATTTTCATACTCAGTCATTTTTTCTATTTTTAAAAATGCTATATTTAGTTCTTAAAGATTTTTGCAATACAAACTTTCATTTCATATTCATTATTCTTATTAACCTTTTTTTTTAATTACAAAGCTTAGTCTTTGAAAACTTTATTCAAGGCTAAGTCAGTGTCTATTTGATGCATGAACGTTAAAACATGTCTGATATACTTCTAATCTTTTAGTTTTTTATTCAATAAGGTTTTAAACTATAGAAATAATGTATAGTTATTTGGTCTTTAATGACTTATTCGATATTTTTTTAATTTATGAAGCCTACTAGATACAAAATTCAAAATTAGGATCCTAATTAGACACAAAATTCATTGAAGTTAATTTTTTTTTTTTAAATATCAAAGGTGTATTAAACAAAAAAAATTGAAATTTAAGAAAATTCATATAATTTAAGAAAATTTTCATAGATAGAAAAAATGTTAAACTATTTACAGAAAATAGCAAAAAAACATTGATAAACATTGATAAACTTCTATCAACATTTATCAGTGATAGACTTCTATCATTTCTATTACTAATAGACTCTGATAGACTTCTGTCAACGTCTATCACAACTATCTAAAAATTTTATTATTTTGTGTAAATAATTTTCTTATTTTTCTATTTTTAAAAACTTTCCTATAATTTAACTCATTTTAAAAAAAAAATTATTTATAGAAACTCGACCTAATACATGCATGCATGTGTGTGTGGTTATTATATTAACTATTATAAAGTCGTGAACATGTAGTAGTAAATTAAGTTCAAAATTTATTAGGTGGCAAACATTTTTTACTATGTAATAAAAGAAGGCAACATGAAAATATGTGCAACTTAGGTTTCACTTAATCATTGTTCTTGTAATTATTAATAATTCAACTACCATATTTCTAATCACTGTGTTTTATGAATGGTTAATTCAGATATTTTGGGCAGCACCAATGACCCATTTGGATGAGACAATATTTGGAGATCCAGAAAAGTTTGAACCAAGTAGATTTGATCAAAACCAAACACCAATTCCACCCTTTAGTTTCATTGCTTTTGGTGGAGGACCAAGAATTTGCCCTGGCTATGAATTTGCAAAGCTTGAAACTCTTGTCACTATCCATTACCTAATCACTCAATTCACTTGGAACCTCACTTCAGACGATTTCTTGACTAGAGATCCTACTTTGATGCCCAACAAAGGTCTCCCCATCCAGATTTATCCCAAATTATAAAACTAATTCATATATTAATCATATATTATTATATGGTAACAACTAGTTTATCATAGTAATGTGGAGATTTGTTACATTCCAAATCCAATACTTATAATGTGGGTGATCACTTTAATTAATATTCTATATTTTCTATCTATATTTATATATAATCTTGTCTTTAATGTCTAATTGTCATTTTATATGTTTGGTCTATAAAGTTGTTGTAAAAGTTATTTCAGAGTTCATTTCATATATATTAATGAAGTTGACTCTCGCCTCATTTTGAATCTATCA

mRNA sequence

ATGATAACCATAATCCTTCTTCTCATCACCATCTTCCTCTTCCTCAGATTTAGATCAACATTCTCCTCCTCCGAGGCCGCGAAAAACCTTCCTCCGGGGTCACTTGGGGTTCCATTTATAGGCCAAAGCCTGAGCCTTCTTCGAGCTATGCGAACAAACTCAGCAGAGCAATGGCTGCAGAAGAGAGTGGCCAAATACGGGCCAGTTTCGAAGATGACGCTGTTCGGGAAGCCGACGGTGTTTGTAAATGGGGCAGCGGCGAATAAGGCGGTGGTGTTTTCGGGGGAGGAGGGGACGGTGTCGAGCCGGCAGATAGAGTCGTTAAAGAGGATTTTGGGAGAGAGGAATTTGACGGAGCTGAGTGGTGAGGATCATAAGAGAGTTAGAGGGGCGCTGGTTTGGTTCTTGAGGCCTCAGAGCTTGAAAGGCTTTGTGGGGAAAATGGATGGAGAAGTGTCACCATTGATGAAAACTCTAACATTTGACATCATATGCTCTCTCCTGTTTGGCATTGAAGAAGGACCAACAAGAAAAAGCATAATAGAATGCTTCAAAACAATGGTGGATGGCATTTGGTCTGTTCCAATCAACTTGCCATTCACAAGGTATAGCCACAGCCTCAAAGCAAGTGCAAAAGCTCAACAGATATTGAAGCAACTTTTGAAAGACAAAACACAGACAATGGAAGAAGAACAACAAGAAGATGATGATAAAGACCTAATTACTTATCTGTTTAGCATCAAGAACAAAGACAAAGAACAAGCTTTGAGTGAGGAGGAAATTGTGCACAATATCATCCTTCTAATGATTGCTGGACATGACACAACATCCATTTTGATTACACTTATGCTTAGGGTTTTGGCTAGAAACCCAACTGTTTATGCAGCTGTTCTTCAAGAGCATGAAGAAATAGCAAGAAACAAAGAAAGTGGGGAGGCACTCAGTTGGGAAGATGTTTCCAAGATGAAGTACACATGGAAAGTGGCAATGGAAACCTTGAGACTTTACCCACCTGTATTTGGAGGGTTTAGAGTGGCCCTTAAAGATATTCAATTAGGTGGATACACAATTCCAAAAGGATGGCAAATATTTTGGGCAGCACCAATGACCCATTTGGATGAGACAATATTTGGAGATCCAGAAAAGTTTGAACCAAGTAGATTTGATCAAAACCAAACACCAATTCCACCCTTTAGTTTCATTGCTTTTGGTGGAGGACCAAGAATTTGCCCTGGCTATGAATTTGCAAAGCTTGAAACTCTTGTCACTATCCATTACCTAATCACTCAATTCACTTGGAACCTCACTTCAGACGATTTCTTGACTAGAGATCCTACTTTGATGCCCAACAAAGGTCTCCCCATCCAGATTTATCCCAAATTATAAAACTAATTCATATATTAATCATATATTATTATATGGTAACAACTAGTTTATCATAGTAATGTGGAGATTTGTTACATTCCAAATCCAATACTTATAATGTGGGTGATCACTTTAATTAATATTCTATATTTTCTATCTATATTTATATATAATCTTGTCTTTAATGTCTAATTGTCATTTTATATGTTTGGTCTATAAAGTTGTTGTAAAAGTTATTTCAGAGTTCATTTCATATATATTAATGAAGTTGACTCTCGCCTCATTTTGAATCTATCA

Coding sequence (CDS)

ATGATAACCATAATCCTTCTTCTCATCACCATCTTCCTCTTCCTCAGATTTAGATCAACATTCTCCTCCTCCGAGGCCGCGAAAAACCTTCCTCCGGGGTCACTTGGGGTTCCATTTATAGGCCAAAGCCTGAGCCTTCTTCGAGCTATGCGAACAAACTCAGCAGAGCAATGGCTGCAGAAGAGAGTGGCCAAATACGGGCCAGTTTCGAAGATGACGCTGTTCGGGAAGCCGACGGTGTTTGTAAATGGGGCAGCGGCGAATAAGGCGGTGGTGTTTTCGGGGGAGGAGGGGACGGTGTCGAGCCGGCAGATAGAGTCGTTAAAGAGGATTTTGGGAGAGAGGAATTTGACGGAGCTGAGTGGTGAGGATCATAAGAGAGTTAGAGGGGCGCTGGTTTGGTTCTTGAGGCCTCAGAGCTTGAAAGGCTTTGTGGGGAAAATGGATGGAGAAGTGTCACCATTGATGAAAACTCTAACATTTGACATCATATGCTCTCTCCTGTTTGGCATTGAAGAAGGACCAACAAGAAAAAGCATAATAGAATGCTTCAAAACAATGGTGGATGGCATTTGGTCTGTTCCAATCAACTTGCCATTCACAAGGTATAGCCACAGCCTCAAAGCAAGTGCAAAAGCTCAACAGATATTGAAGCAACTTTTGAAAGACAAAACACAGACAATGGAAGAAGAACAACAAGAAGATGATGATAAAGACCTAATTACTTATCTGTTTAGCATCAAGAACAAAGACAAAGAACAAGCTTTGAGTGAGGAGGAAATTGTGCACAATATCATCCTTCTAATGATTGCTGGACATGACACAACATCCATTTTGATTACACTTATGCTTAGGGTTTTGGCTAGAAACCCAACTGTTTATGCAGCTGTTCTTCAAGAGCATGAAGAAATAGCAAGAAACAAAGAAAGTGGGGAGGCACTCAGTTGGGAAGATGTTTCCAAGATGAAGTACACATGGAAAGTGGCAATGGAAACCTTGAGACTTTACCCACCTGTATTTGGAGGGTTTAGAGTGGCCCTTAAAGATATTCAATTAGGTGGATACACAATTCCAAAAGGATGGCAAATATTTTGGGCAGCACCAATGACCCATTTGGATGAGACAATATTTGGAGATCCAGAAAAGTTTGAACCAAGTAGATTTGATCAAAACCAAACACCAATTCCACCCTTTAGTTTCATTGCTTTTGGTGGAGGACCAAGAATTTGCCCTGGCTATGAATTTGCAAAGCTTGAAACTCTTGTCACTATCCATTACCTAATCACTCAATTCACTTGGAACCTCACTTCAGACGATTTCTTGACTAGAGATCCTACTTTGATGCCCAACAAAGGTCTCCCCATCCAGATTTATCCCAAATTATAA

Protein sequence

MITIILLLITIFLFLRFRSTFSSSEAAKNLPPGSLGVPFIGQSLSLLRAMRTNSAEQWLQKRVAKYGPVSKMTLFGKPTVFVNGAAANKAVVFSGEEGTVSSRQIESLKRILGERNLTELSGEDHKRVRGALVWFLRPQSLKGFVGKMDGEVSPLMKTLTFDIICSLLFGIEEGPTRKSIIECFKTMVDGIWSVPINLPFTRYSHSLKASAKAQQILKQLLKDKTQTMEEEQQEDDDKDLITYLFSIKNKDKEQALSEEEIVHNIILLMIAGHDTTSILITLMLRVLARNPTVYAAVLQEHEEIARNKESGEALSWEDVSKMKYTWKVAMETLRLYPPVFGGFRVALKDIQLGGYTIPKGWQIFWAAPMTHLDETIFGDPEKFEPSRFDQNQTPIPPFSFIAFGGGPRICPGYEFAKLETLVTIHYLITQFTWNLTSDDFLTRDPTLMPNKGLPIQIYPKL

Homology

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BLAST of Lsi04G012100 vs. ExPASy Swiss-Prot
Match: Q6WG30 (Taxadiene 5-alpha hydroxylase OS=Taxus cuspidata OX=99806 PE=1 SV=2)

HSP 1 Score: 327.8 bits (839), Expect = 2.0e-88
Identity = 179/469 (38.17%), Postives = 280/469 (59.70%), Query Frame = 0


Query: 11  IFLFLRFRSTFSSSEAAKNLPPGSLGVPFIGQSLSLLRAMRTNSAEQWLQKRVAKYGPVS 70
           + L L FRS   SS     LPPG LG+PFIG+S   LRA+R+NS EQ+  +RV K+G V 
Sbjct: 36  LLLLLLFRSKRHSS---LKLPPGKLGIPFIGESFIFLRALRSNSLEQFFDERVKKFGLVF 95

Query: 71  KMTLFGKPTVFVNGAAANKAVVFSGEEGTVSSRQIESLKRILGERNLTELSGEDHKRVRG 130
           K +L G PTV + G A N+ ++ S EE  V         +++GE ++    GEDH  +R 
Sbjct: 96  KTSLIGHPTVVLCGPAGNR-LILSNEEKLVQMSWPAQFMKLMGENSVATRRGEDHIVMRS 155

Query: 131 ALVWFLRPQSLKGFVGKMDGEVS----------------PLMKTLTFDIICSLLFGIEEG 190
           AL  F  P +L+ ++GKM+ E+                 PL++ L F+I   L F I + 
Sbjct: 156 ALAGFFGPGALQSYIGKMNTEIQSHINEKWKGKDEVNVLPLVRELVFNISAILFFNIYDK 215

Query: 191 PTRKSIIECFKTMVDGIWSVPINLPFTRYSHSLKASAKAQQILKQLLKDKTQTMEEEQQE 250
             +  + +  +T++ G +++PI+LP   +  +L+  AK  +I+  L+K + + + +    
Sbjct: 216 QEQDRLHKLLETILVGSFALPIDLPGFGFHRALQGRAKLNKIMLSLIKKRKEDL-QSGSA 275

Query: 251 DDDKDLITYLFSIKNKDKEQALSEEEIVHNIILLMIAGHDTTSILITLMLRVLARNPTVY 310
              +DL++ L + ++ DK   L+ +EI+ N   L+ A +DTT+  + L+ ++L+ NP  Y
Sbjct: 276 TATQDLLSVLLTFRD-DKGTPLTNDEILDNFSSLLHASYDTTTSPMALIFKLLSSNPECY 335

Query: 311 AAVLQEHEEIARNKESGEALSWEDVSKMKYTWKVAMETLRLYPPVFGGFRVALKDIQLGG 370
             V+QE  EI  NKE GE ++W+D+  MKYTW+VA ETLR++PPVFG FR A+ DIQ  G
Sbjct: 336 QKVVQEQLEILSNKEEGEEITWKDLKAMKYTWQVAQETLRMFPPVFGTFRKAITDIQYDG 395

Query: 371 YTIPKGWQIFWAAPMTHLDETIFGDPEKFEPSRFDQNQTPIPPFSFIAFGGGPRICPGYE 430
           YTIPKGW++ W    TH  +  F +PEKF PSRFDQ    + P++F+ FGGG R C G+E
Sbjct: 396 YTIPKGWKLLWTTYSTHPKDLYFNEPEKFMPSRFDQEGKHVAPYTFLPFGGGQRSCVGWE 455

Query: 431 FAKLETLVTIHYLITQFT--WNLTSDDFLTRDP-TLMPNKGLPIQIYPK 461
           F+K+E L+ +H+ +  F+    +  D+ ++ DP   +P+KG  I+++P+
Sbjct: 456 FSKMEILLFVHHFVKTFSSYTPVDPDEKISGDPLPPLPSKGFSIKLFPR 498

[Close]

BLAST of Lsi04G012100 vs. ExPASy Swiss-Prot
Match: Q9AXM6 (Taxane 10-beta-hydroxylase OS=Taxus cuspidata OX=99806 GN=CYP725A1 PE=1 SV=1)

HSP 1 Score: 314.7 bits (805), Expect = 1.7e-84
Identity = 171/472 (36.23%), Postives = 274/472 (58.05%), Query Frame = 0


Query: 8   LITIFLFLRFRSTFSSSEAAKNLPPGSLGVPFIGQSLSLLRAMRTNSAEQWLQKRVAKYG 67
           ++ I L L FR    SS     LPPG LG P IG+++ LLR +R+ + +++   R+ K+G
Sbjct: 31  ILAIILLLLFRYNHRSS---VKLPPGKLGFPLIGETIQLLRTLRSETPQKFFDDRLKKFG 90

Query: 68  PVSKMTLFGKPTVFVNGAAANKAVVFSGEEGTVSSRQIESLKRILGERNLTELSGEDHKR 127
           PV   +L G PTV + G A NK +V S E+  V     +S  +++GE ++    GEDH+ 
Sbjct: 91  PVYMTSLIGHPTVVLCGPAGNK-LVLSNEDKLVEMEGPKSFMKLIGEDSIVAKRGEDHRI 150

Query: 128 VRGALVWFLRPQSLKGFVGKMDGEVS----------------PLMKTLTFDIICSLLFGI 187
           +R AL  FL  Q+L+ ++G+M  E+                 PL++ L F I  +L F +
Sbjct: 151 LRTALARFLGAQALQNYLGRMSSEIGHHFNEKWKGKDEVKVLPLVRGLIFSIASTLFFDV 210

Query: 188 EEGPTRKSIIECFKTMVDGIWSVPINLPFTRYSHSLKASAKAQQILKQLLKDKTQTMEEE 247
            +G  +K +    +T++ G  SVP++ P TRY   L+A  K  +IL  L+K + + +   
Sbjct: 211 NDGHQQKQLHHLLETILVGSLSVPLDFPGTRYRKGLQARLKLDEILSSLIKRRRRDL-RS 270

Query: 248 QQEDDDKDLITYLFSIKNKDKEQALSEEEIVHNIILLMIAGHDTTSILITLMLRVLARNP 307
               DD+DL++ L + ++ +K  +L+++ I+ N   +  A +DTT   + L+ ++L  NP
Sbjct: 271 GIASDDQDLLSVLLTFRD-EKGNSLTDQGILDNFSAMFHASYDTTVAPMALIFKLLYSNP 330

Query: 308 TVYAAVLQEHEEIARNKESGEALSWEDVSKMKYTWKVAMETLRLYPPVFGGFRVALKDIQ 367
             +  V QE  EI  NK+ GE +SW+D+  MKYTW+   E+LR+YPPVFG FR A+ DI 
Sbjct: 331 EYHEKVFQEQLEIIGNKKEGEEISWKDLKSMKYTWQAVQESLRMYPPVFGIFRKAITDIH 390

Query: 368 LGGYTIPKGWQIFWAAPMTHLDETIFGDPEKFEPSRFDQNQTPIPPFSFIAFGGGPRICP 427
             GYTIPKGW++  +   THL E  F +PE+F PSRF+     + P++++ FGGG R CP
Sbjct: 391 YDGYTIPKGWRVLCSPYTTHLREEYFPEPEEFRPSRFEDEGRHVTPYTYVPFGGGLRTCP 450

Query: 428 GYEFAKLETLVTIHYLITQFTWNLTSD--DFLTRDP-TLMPNKGLPIQIYPK 461
           G+EF+K+E L+ +H+ +  F+  +  D  + +  DP   +P  G  I+++P+
Sbjct: 451 GWEFSKIEILLFVHHFVKNFSSYIPVDPNEKVLSDPLPPLPANGFSIKLFPR 496

[Close]

BLAST of Lsi04G012100 vs. ExPASy Swiss-Prot
Match: Q84KI1 (Taxoid 14-beta-hydroxylase OS=Taxus cuspidata OX=99806 PE=1 SV=1)

HSP 1 Score: 312.0 bits (798), Expect = 1.1e-83
Identity = 171/473 (36.15%), Postives = 269/473 (56.87%), Query Frame = 0


Query: 7   LLITIFLFLRFRSTFSSSEAAKNLPPGSLGVPFIGQSLSLLRAMRTNSAEQWLQKRVAKY 66
           +++ + LFLR     S   ++  LPPG LG PFIG+SL  L+A+R+N+ EQ+L +RV  +
Sbjct: 32  IVLPLLLFLR-----SKRRSSVGLPPGKLGYPFIGESLLFLKALRSNTVEQFLDERVKNF 91

Query: 67  GPVSKMTLFGKPTVFVNGAAANKAVVFSGEEGTVSSRQIESLKRILGERNLTELSGEDHK 126
           G V K +L G PTV + G A N+ ++ + EE  V     +S  +++GE+++T   GE H 
Sbjct: 92  GNVFKTSLIGHPTVVLCGPAGNR-LILANEEKLVQMSWPKSSMKLMGEKSITAKRGEGHM 151

Query: 127 RVRGALVWFLRPQSLKGFVGKMDG----------------EVSPLMKTLTFDIICSLLFG 186
            +R AL  F  P +L+ ++G+M                   V  L+  L FDI   L F 
Sbjct: 152 IIRSALQGFFSPGALQKYIGQMSKTIENHINEKWKGNDQVSVVALVGDLVFDISACLFFN 211

Query: 187 IEEGPTRKSIIECFKTMVDGIWSVPINLPFTRYSHSLKASAKAQQILKQLLKDKTQTMEE 246
           I E   R+ + E  + +  G+ +VP++LP   Y  +L+A +K   IL  L++ +   +  
Sbjct: 212 INEKHERERLFELLEIIAVGVLAVPVDLPGFAYHRALQARSKLNAILSGLIEKRKMDL-S 271

Query: 247 EQQEDDDKDLITYLFSIKNKDKEQALSEEEIVHNIILLMIAGHDTTSILITLMLRVLARN 306
                 ++DL++   + K+ D+    S+EEI+ N   L+   +DTT   +  + ++L+ N
Sbjct: 272 SGLATSNQDLLSVFLTFKD-DRGNPCSDEEILDNFSGLLHGSYDTTVSAMACVFKLLSSN 331

Query: 307 PTVYAAVLQEHEEIARNKESGEALSWEDVSKMKYTWKVAMETLRLYPPVFGGFRVALKDI 366
           P  Y  V+QE   I  NK  G+ ++W+DV  MKYTW+V  ETLRLYP +FG FR A+ DI
Sbjct: 332 PECYEKVVQEQLGILSNKLEGDEITWKDVKSMKYTWQVVQETLRLYPSIFGSFRQAITDI 391

Query: 367 QLGGYTIPKGWQIFWAAPMTHLDETIFGDPEKFEPSRFDQNQTPIPPFSFIAFGGGPRIC 426
              GY IPKGW++ W    TH  E  F +PEKF PSRFDQ    + P++F+ FGGG R C
Sbjct: 392 HYNGYIIPKGWKLLWTPYTTHPKEMYFSEPEKFLPSRFDQEGKLVAPYTFLPFGGGQRSC 451

Query: 427 PGYEFAKLETLVTIHYLITQFTWNLTSD--DFLTRDPTL-MPNKGLPIQIYPK 461
           PG+EF+K+E L+++H+ +  F+     D  + + RD    +P+ G  ++++P+
Sbjct: 452 PGWEFSKMEILLSVHHFVKTFSTFTPVDPAEIIARDSLCPLPSNGFSVKLFPR 496

[Close]

BLAST of Lsi04G012100 vs. ExPASy Swiss-Prot
Match: Q50EK1 (Cytochrome P450 716B1 OS=Picea sitchensis OX=3332 GN=CYP716B1 PE=2 SV=1)

HSP 1 Score: 302.4 bits (773), Expect = 8.9e-81
Identity = 174/477 (36.48%), Postives = 267/477 (55.97%), Query Frame = 0


Query: 1   MITIILLLITIFLFLRFRSTFSSSEAAKNLPPGSLGVPFIGQSLSLLRAMRTNSAEQWLQ 60
           M T+ L       F  +  +   ++A + +PPG+ G P IG++L  L   R  +   +  
Sbjct: 22  MFTVFLASFIGLAFFFYLISNHRTKAWRGIPPGTFGWPLIGETLEFLGCQRKGNPRDFFD 81

Query: 61  KRVAKYGPVSKMTLFGKPTVFVNGAAANKAVVFSGEEGTVSSRQIESLKRILGERNLTEL 120
            R  KYG V   +L G PTV       N+  +FS E   V +    S+  +     +T +
Sbjct: 82  SRTQKYGNVFTTSLVGHPTVVFCSPEGNR-FLFSNENKLVVNSWPSSVGNLFRSSLITTV 141

Query: 121 SGEDHKRVRGALVWFLRPQSLKGFVGKMDGEVS----------------PLMKTLTFDII 180
            G+D KR+R  L+ FLRP++L+ FVG++D                    PL+K  TF + 
Sbjct: 142 -GDDAKRLRRILMTFLRPEALREFVGRVDSMTKRHLAEHWIGKDEVMALPLLKRYTFSLA 201

Query: 181 CSLLFGIEEGPTRKSIIECFKTMVDGIWSVPINLPFTRYSHSLKASAKAQQILKQLLKDK 240
           C L   I        +   F   V G+  +PI+LP TRY+ +  A+   +Q L  ++ ++
Sbjct: 202 CDLFASINTKDDLDRLWLHFMVFVKGVMQIPIDLPGTRYNKTKHAANAIRQQLGSIINER 261

Query: 241 TQTMEEEQQEDDDKDLITYLFSIKNKDKE-QALSEEEIVHNIILLMIAGHDTTSILITLM 300
              + E      ++DL+++L S  N D++ ++L++ EI  NI+LL+ AGHDT+S  +T++
Sbjct: 262 KIAL-EAGNASPEQDLLSFLLS--NVDEQGESLTDNEIQDNILLLLYAGHDTSSSTLTVL 321

Query: 301 LRVLARNPTVYAAVLQEHEEIARNKESGEALSWEDVSKMKYTWKVAMETLRLYPPVFGGF 360
           L+ LA NP  Y  VL+E   IA +KE G+ L WED+ +MKY+W+VA E LRL+P V G F
Sbjct: 322 LKFLAENPHCYEEVLREQLNIAGSKEEGQLLEWEDLQRMKYSWRVAQEALRLFPAVQGSF 381

Query: 361 RVALKDIQLGGYTIPKGWQIFWAAPMTHLDETIFGDPEKFEPSRFDQNQTPIPPFSFIAF 420
           R A+K+    G+TIPKGW++ W    TH     F +PEKF+PSRF + + P PP++F+ F
Sbjct: 382 RKAIKEFIYDGFTIPKGWKLHWTVNSTHQKSEYFSNPEKFDPSRF-EGEGP-PPYTFVPF 441

Query: 421 GGGPRICPGYEFAKLETLVTIHYLITQFTWNLTSD-DFLTRDPTLMPNKGLPIQIYP 460
           GGGPR+CPG EFA++E L+ +H ++  F WNL +  + +  DP   P  GLPI++ P
Sbjct: 442 GGGPRMCPGNEFARMEILIFLHNIVKNFNWNLVNPLEKVIVDPMPAPVNGLPIKLVP 491

[Close]

BLAST of Lsi04G012100 vs. ExPASy Swiss-Prot
Match: Q50EK0 (Cytochrome P450 716B2 OS=Picea sitchensis OX=3332 GN=CYP716B2 PE=2 SV=1)

HSP 1 Score: 298.1 bits (762), Expect = 1.7e-79
Identity = 175/477 (36.69%), Postives = 270/477 (56.60%), Query Frame = 0


Query: 1   MITIILLLITIFLFLRFRSTFSSSEAAKNLPPGSLGVPFIGQSLSLLRAMRTNSAEQWLQ 60
           M T+ L       F  +  +   ++A + +PPG+ G P IG++L  L   R  +   +  
Sbjct: 22  MFTVFLASFIGLAFFFYLISNHRTKAWRGIPPGTFGWPLIGETLEFLGCQRKGNPRDFFD 81

Query: 61  KRVAKYGPVSKMTLFGKPTVFVNGAAANKAVVFSGEEGTVSSRQIESLKRILGERNLTEL 120
            R  KYG V   +L G PTV       N+  +FS E   V +    S+  +     +T +
Sbjct: 82  SRTQKYGNVFTTSLVGHPTVVFCSPEGNR-FLFSNENKLVVNSWPSSVGNLFRSSLITTV 141

Query: 121 SGEDHKRVRGALVWFLRPQSLKGFVGKMDG--------------EVS--PLMKTLTFDII 180
            G+D KR+R  L+ FLRP++L+ FVG++D               EV+  PL+K  TF + 
Sbjct: 142 -GDDAKRLRRILMTFLRPEALREFVGRVDSMTKRHLAEHWIGKDEVTALPLLKRYTFSLA 201

Query: 181 CSLLFGIEEGPTRKSIIECFKTMVDGIWSVPINLPFTRYSHSLKASAKAQQILKQLLKDK 240
           C L   I        +   F   V G+  +PI+LP TRY+ +  A+   +Q L  ++ ++
Sbjct: 202 CDLFASINNKDDLGRLWLHFMVFVKGVMQIPIDLPGTRYNKAKHAANAIRQQLGSIINER 261

Query: 241 TQTMEEEQQEDDDKDLITYLFSIKNKDKE-QALSEEEIVHNIILLMIAGHDTTSILITLM 300
              + E      ++DL+++L S  N D++ ++L++ EI  NI+LL+ AGHDT+S  +T++
Sbjct: 262 KIGL-EAGNASPEQDLLSFLLS--NVDEQGESLTDNEIQDNILLLLYAGHDTSSSTLTVL 321

Query: 301 LRVLARNPTVYAAVLQEHEEIARNKESGEALSWEDVSKMKYTWKVAMETLRLYPPVFGGF 360
           L+ LA NP  Y  VL+E  +IA +KE+G+ L WED+ +MKY+W+VA E LRL+P   G F
Sbjct: 322 LKFLAENPHCYEEVLREQLDIAGSKEAGQLLEWEDLQRMKYSWRVAQEALRLFPAAQGSF 381

Query: 361 RVALKDIQLGGYTIPKGWQIFWAAPMTHLDETIFGDPEKFEPSRFDQNQTPIPPFSFIAF 420
           R A+K+    G+TIPKGW+++W    TH     F +PE F+PSRF + + P PP++F+ F
Sbjct: 382 RKAIKEFIYDGFTIPKGWKMYWTVNSTHRKSEYFSNPETFDPSRF-EGEGP-PPYTFVPF 441

Query: 421 GGGPRICPGYEFAKLETLVTIHYLITQFTWNLTS-DDFLTRDPTLMPNKGLPIQIYP 460
           GGGPR+CPG EFA+LE LV +H ++    WNL +  + +  DP   P  GLPI++ P
Sbjct: 442 GGGPRMCPGNEFARLEILVFLHNIVKNCKWNLVNPGEKVIVDPMPAPVNGLPIKLVP 491

[Close]

BLAST of Lsi04G012100 vs. ExPASy TrEMBL
Match: A0A0A0L864 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G416660 PE=3 SV=1)

HSP 1 Score: 804.3 bits (2076), Expect = 2.7e-229
Identity = 411/481 (85.45%), Postives = 442/481 (91.89%), Query Frame = 0


Query: 1   MITIILLLI-TIFLFLRFRSTFSSSEAAKNLPPGSLGVPFIGQSLSLLRAMRTNSAEQWL 60
           MIT+I+LL+ TIFLF RFR+  SSS AAKNLPPG+LGVPFIGQSLSLL AMRTN+AE+WL
Sbjct: 1   MITVIILLVLTIFLFHRFRTRLSSS-AAKNLPPGALGVPFIGQSLSLLGAMRTNTAEKWL 60

Query: 61  QKRVAKYGPVSKMTLFGKPTVFVNGAAANKAVVFSGEEGTVSSRQIESLKRILGERNLTE 120
           QKRV KYGPVSKMTLFGKPTVF++GAAANKAVVFSGEEGTVS+RQ+ESLK ILG+RNLTE
Sbjct: 61  QKRVDKYGPVSKMTLFGKPTVFIHGAAANKAVVFSGEEGTVSNRQVESLKMILGKRNLTE 120

Query: 121 LSGEDHKRVRGALVWFLRPQSLKGFVGKMDGE----------------VSPLMKTLTFDI 180
           LSGEDHKRVRGALVWFLRPQ+L+ +VGKMDGE                V+PLMKTLTFDI
Sbjct: 121 LSGEDHKRVRGALVWFLRPQTLRSYVGKMDGEVRRHLNMYWHGNNEVTVAPLMKTLTFDI 180

Query: 181 ICSLLFGIEEGPTRKSIIECFKTMVDGIWSVPINLPFTRYSHSLKASAKAQQILKQLLKD 240
           ICSLLFGIEEG TRKSIIECFKTMVDGIWS+PINLPFTRY+HSLKASAKAQQILKQLLKD
Sbjct: 181 ICSLLFGIEEGTTRKSIIECFKTMVDGIWSIPINLPFTRYNHSLKASAKAQQILKQLLKD 240

Query: 241 KTQTMEEEQQ---EDDDKDLITYLFSIKNKDKEQALSEEEIVHNIILLMIAGHDTTSILI 300
           K + MEEE++   E++DKDLI+YL  IKNKDKEQALSEEEIVHNIILLMIAGHDTTSIL+
Sbjct: 241 KAKVMEEEKESEVEENDKDLISYLLRIKNKDKEQALSEEEIVHNIILLMIAGHDTTSILL 300

Query: 301 TLMLRVLARNPTVYAAVLQEHEEIARNKESGEALSWEDVSKMKYTWKVAMETLRLYPPVF 360
           TLMLRVLA NPTVYAAVLQEHEEI R+KE GEAL+WEDVSKMKYTW+VAMETLRLYPPVF
Sbjct: 301 TLMLRVLATNPTVYAAVLQEHEEIGRSKERGEALTWEDVSKMKYTWRVAMETLRLYPPVF 360

Query: 361 GGFRVALKDIQLGGYTIPKGWQIFWAAPMTHLDETIFGDPEKFEPSRFDQNQTPIPPFSF 420
           GGFRVALKDIQLG YTIPKGWQIFWAAPMTHLDETIFGDP+KFEPSRFDQNQTPIPPFSF
Sbjct: 361 GGFRVALKDIQLGAYTIPKGWQIFWAAPMTHLDETIFGDPQKFEPSRFDQNQTPIPPFSF 420

Query: 421 IAFGGGPRICPGYEFAKLETLVTIHYLITQFTWNLT-SDDFLTRDPTLMPNKGLPIQIYP 461
           IAFGGGPRICPGYEFAKLETLVTIHYLITQFTWNL+ S DFLTRDPTLMPNKGLPIQI+P
Sbjct: 421 IAFGGGPRICPGYEFAKLETLVTIHYLITQFTWNLSCSQDFLTRDPTLMPNKGLPIQIFP 480

[Close]

BLAST of Lsi04G012100 vs. ExPASy TrEMBL
Match: A0A6J1INM7 (taxadiene 5-alpha hydroxylase-like OS=Cucurbita maxima OX=3661 GN=LOC111476875 PE=3 SV=1)

HSP 1 Score: 795.0 bits (2052), Expect = 1.6e-226
Identity = 406/479 (84.76%), Postives = 433/479 (90.40%), Query Frame = 0


Query: 1   MITIILLLITIFLFLRFRSTFSSSEAAKNLPPGSLGVPFIGQSLSLLRAMRTNSAEQWLQ 60
           MI  ILLLITIFL+LR R+TF SS  AKNLPPG LGVPFIGQSLSLLRAMR NSAEQWLQ
Sbjct: 1   MIATILLLITIFLYLRLRATFFSSAEAKNLPPGPLGVPFIGQSLSLLRAMRANSAEQWLQ 60

Query: 61  KRVAKYGPVSKMTLFGKPTVFVNGAAANKAVVFSGEEGTVSSRQIESLKRILGERNLTEL 120
           +RVAKYGP+SKMTLFGKPTVFV+G AANKAVVFSGEE  VS+RQ ESL+RILGERNLTEL
Sbjct: 61  QRVAKYGPISKMTLFGKPTVFVHGVAANKAVVFSGEEEAVSNRQTESLRRILGERNLTEL 120

Query: 121 SGEDHKRVRGALVWFLRPQSLKGFVGKMDGE----------------VSPLMKTLTFDII 180
           SGEDHKRVRGALVWFLRPQSLKGFVGKMDGE                V+PLMKTLTFDII
Sbjct: 121 SGEDHKRVRGALVWFLRPQSLKGFVGKMDGEVRKHLNLHWHGNKEVIVAPLMKTLTFDII 180

Query: 181 CSLLFGIEEGPTRKSIIECFKTMVDGIWSVPINLPFTRYSHSLKASAKAQQILKQLLKDK 240
           CSLLFGIEEGPTRKSIIECFKTMVDGIWSVPINLPFTRYSHSL ASAK QQILKQLLKDK
Sbjct: 181 CSLLFGIEEGPTRKSIIECFKTMVDGIWSVPINLPFTRYSHSLNASAKVQQILKQLLKDK 240

Query: 241 TQTMEEEQQE-DDDKDLITYLFSIKNKDKEQALSEEEIVHNIILLMIAGHDTTSILITLM 300
            Q MEEE++E ++DK+LIT L SIKNKDKEQALSEEEIVHNIILL+IAGHDTTSILITLM
Sbjct: 241 AQKMEEEEEEKEEDKNLITCLLSIKNKDKEQALSEEEIVHNIILLLIAGHDTTSILITLM 300

Query: 301 LRVLARNPTVYAAVLQEHEEIARNKESGEALSWEDVSKMKYTWKVAMETLRLYPPVFGGF 360
           LRV+A NPTVYAAVLQEHE+I R+KE GEAL+WEDVSKMKYTW+VA+ETLRL+PPVFGGF
Sbjct: 301 LRVMATNPTVYAAVLQEHEDIHRSKECGEALTWEDVSKMKYTWRVALETLRLFPPVFGGF 360

Query: 361 RVALKDIQLGGYTIPKGWQIFWAAPMTHLDETIFGDPEKFEPSRFDQNQTPI-PPFSFIA 420
           RVALKDIQLGGYTIPKGWQIFWAA MTHLDE IF DP+KF+PSRFDQNQT I PP SFIA
Sbjct: 361 RVALKDIQLGGYTIPKGWQIFWAASMTHLDEKIFTDPQKFKPSRFDQNQTSIPPPLSFIA 420

Query: 421 FGGGPRICPGYEFAKLETLVTIHYLITQFTWNLTSDDFLTRDPTLMPNKGLPIQIYPKL 462
           FGGGPRICPGYEFAKLETLVTIHY+ITQFTW L+S DFLTRDP+LMPNKGLPIQ+YPKL
Sbjct: 421 FGGGPRICPGYEFAKLETLVTIHYIITQFTWKLSSQDFLTRDPSLMPNKGLPIQLYPKL 479

[Close]

BLAST of Lsi04G012100 vs. ExPASy TrEMBL
Match: A0A6J1F7G6 (taxadiene 5-alpha hydroxylase-like OS=Cucurbita moschata OX=3662 GN=LOC111443055 PE=3 SV=1)

HSP 1 Score: 793.9 bits (2049), Expect = 3.6e-226
Identity = 406/480 (84.58%), Postives = 437/480 (91.04%), Query Frame = 0


Query: 1   MITIILLLITIFLFLRFRST-FSSSEAAKNLPPGSLGVPFIGQSLSLLRAMRTNSAEQWL 60
           MI IILLLITIF++LR R+T FS++ AAKNLPPG LGVP+IGQSLSLLRAMR NSAEQWL
Sbjct: 1   MIAIILLLITIFVYLRLRTTFFSTAVAAKNLPPGRLGVPYIGQSLSLLRAMRANSAEQWL 60

Query: 61  QKRVAKYGPVSKMTLFGKPTVFVNGAAANKAVVFSGEEGTVSSRQIESLKRILGERNLTE 120
           Q+RVAKYGP+SKMTLFGKPTVFV+G AANKAVVFSGEE  VS+RQ ESL+RILGERNLTE
Sbjct: 61  QQRVAKYGPISKMTLFGKPTVFVHGVAANKAVVFSGEEEAVSNRQTESLRRILGERNLTE 120

Query: 121 LSGEDHKRVRGALVWFLRPQSLKGFVGKMDGE----------------VSPLMKTLTFDI 180
           LSGEDHKRVRGALVWFLRPQSLKGFVGKMDGE                V+PLMKTLTFDI
Sbjct: 121 LSGEDHKRVRGALVWFLRPQSLKGFVGKMDGEVRKHLKLHWHGNKEVIVAPLMKTLTFDI 180

Query: 181 ICSLLFGIEEGPTRKSIIECFKTMVDGIWSVPINLPFTRYSHSLKASAKAQQILKQLLKD 240
           ICSLLFGIEEGPTRKSIIECFKTMVDGIWSVPINLPFTRYSHSLKASAK QQILKQLLKD
Sbjct: 181 ICSLLFGIEEGPTRKSIIECFKTMVDGIWSVPINLPFTRYSHSLKASAKVQQILKQLLKD 240

Query: 241 KTQTME-EEQQEDDDKDLITYLFSIKNKDKEQALSEEEIVHNIILLMIAGHDTTSILITL 300
           K Q ME EE+++++DK+LIT L SIKNKDKEQALSEEEIVHNIILL+IAGHDTTSILITL
Sbjct: 241 KAQKMEGEEEEKEEDKNLITCLLSIKNKDKEQALSEEEIVHNIILLLIAGHDTTSILITL 300

Query: 301 MLRVLARNPTVYAAVLQEHEEIARNKESGEALSWEDVSKMKYTWKVAMETLRLYPPVFGG 360
           MLRV+A NPTVYAAVLQEHE+I R+KE GEAL+WEDVSKMKYTW+VA+ETLRL+PPVFGG
Sbjct: 301 MLRVMATNPTVYAAVLQEHEDILRSKECGEALTWEDVSKMKYTWRVALETLRLFPPVFGG 360

Query: 361 FRVALKDIQLGGYTIPKGWQIFWAAPMTHLDETIFGDPEKFEPSRFDQNQTPI-PPFSFI 420
           FRVALKDIQLGGYTIPKGWQIFWAA MTHLDE IF DP+KFEPSRFDQNQT I PP SFI
Sbjct: 361 FRVALKDIQLGGYTIPKGWQIFWAASMTHLDEKIFRDPQKFEPSRFDQNQTSIPPPLSFI 420

Query: 421 AFGGGPRICPGYEFAKLETLVTIHYLITQFTWNLTSDDFLTRDPTLMPNKGLPIQIYPKL 462
           AFGGGPRICPGYEFAKLETLVTIHY+ITQFTW L+S DFLTRDP+LMPNKGLPIQ+YPKL
Sbjct: 421 AFGGGPRICPGYEFAKLETLVTIHYIITQFTWKLSSQDFLTRDPSLMPNKGLPIQLYPKL 480

[Close]

BLAST of Lsi04G012100 vs. ExPASy TrEMBL
Match: A0A1S3C037 (LOW QUALITY PROTEIN: taxadiene 5-alpha hydroxylase-like OS=Cucumis melo OX=3656 GN=LOC103494945 PE=3 SV=1)

HSP 1 Score: 777.3 bits (2006), Expect = 3.5e-221
Identity = 403/479 (84.13%), Postives = 434/479 (90.61%), Query Frame = 0


Query: 1   MIT-IILLLITIFLFLRFRSTFSSSEAAKNLPPGSLGVPFIGQSLSLLRAMRTNSAEQWL 60
           MIT IILL+ITIFLF RFR+ FSSS AAKNLPPG+LGVPFIGQSLSLL AMRTN+AE+WL
Sbjct: 1   MITPIILLVITIFLFHRFRTRFSSS-AAKNLPPGALGVPFIGQSLSLLGAMRTNTAEKWL 60

Query: 61  QKRVAKYGPVSKMTLFGKPTVFVNGAAANKAVVFSGEEGTVSSRQIESLKRILGERNLTE 120
           Q+RV KYGPVSKMTLFGKPTVFV+GAAANKAVVFSGEEGTVS+RQIESLK ILG+RNLTE
Sbjct: 61  QQRVDKYGPVSKMTLFGKPTVFVHGAAANKAVVFSGEEGTVSNRQIESLKMILGKRNLTE 120

Query: 121 LSGEDHKRVRGALVWFLRPQSLKGFVGKMDGE----------------VSPLMKTLTFDI 180
           LSGEDHKRVRGALVWFLRPQ+L+ +V KMDGE                V+PLMKTLTFDI
Sbjct: 121 LSGEDHKRVRGALVWFLRPQTLRSYVEKMDGEVRRHLNMYWHGNNKVTVAPLMKTLTFDI 180

Query: 181 ICSLLFGIEEGPTRKSIIECFKTMVDGIWSVPINLPFTRYSHSLKASAKAQQILKQLLKD 240
           ICSLLFGIEEG TRKSII+CFKTMV+GIWSVPINLPFTRY+HSLKASAK QQILKQLLKD
Sbjct: 181 ICSLLFGIEEGTTRKSIIQCFKTMVEGIWSVPINLPFTRYNHSLKASAKVQQILKQLLKD 240

Query: 241 KTQTMEEEQQ-EDDDKDLITYLFSIKNKDKEQALSEEEIVHNIILLMIAGHDTTSILITL 300
           K + ME++++ +   KDLITYL  IKNKDKEQAL+EEEI+HNIILLMIAGHDTTSIL+TL
Sbjct: 241 KAKRMEQKKKMKKKMKDLITYLLRIKNKDKEQALTEEEILHNIILLMIAGHDTTSILLTL 300

Query: 301 MLRVLARNPTVYAAVLQEHEEIARNKESGEALSWEDVSKMKYTWKVAMETLRLYPPVFGG 360
           MLRVLA NPTVYA VLQEHEEIAR+KE GEAL+WEDVSKMKYTW+VAMETLRLYPPVFGG
Sbjct: 301 MLRVLATNPTVYADVLQEHEEIARSKERGEALTWEDVSKMKYTWRVAMETLRLYPPVFGG 360

Query: 361 FRVALKDIQLGGYTIPKGWQIFWAAPMTHLDETIFGDPEKFEPSRFDQNQTPIPPFSFIA 420
           FRVALKDIQLG YTIPKGWQIFWAA  THLDETIFGDP+KFEPSRFDQ QTPIPPFSFIA
Sbjct: 361 FRVALKDIQLGAYTIPKGWQIFWAASTTHLDETIFGDPQKFEPSRFDQTQTPIPPFSFIA 420

Query: 421 FGGGPRICPGYEFAKLETLVTIHYLITQFTWNLT-SDDFLTRDPTLMPNKGLPIQIYPK 461
           FGGGPRICPGYEFAKLETLVTIHYLITQFTWNL+ S D LTRDPTLMPNKGLPIQI+PK
Sbjct: 421 FGGGPRICPGYEFAKLETLVTIHYLITQFTWNLSCSQDSLTRDPTLMPNKGLPIQIFPK 478

[Close]

BLAST of Lsi04G012100 vs. ExPASy TrEMBL
Match: A0A6J1CCZ3 (taxadiene 5-alpha hydroxylase-like OS=Momordica charantia OX=3673 GN=LOC111010356 PE=3 SV=1)

HSP 1 Score: 715.7 bits (1846), Expect = 1.3e-202
Identity = 362/472 (76.69%), Postives = 404/472 (85.59%), Query Frame = 0


Query: 5   ILLLITIFLFLRFRSTFSSSEAAKNLPPGSLGVPFIGQSLSLLRAMRTNSAEQWLQKRVA 64
           + LL   FLFL  R T SS      LPPG LG+P+IGQSL LLRAMR+NSAEQWL +RVA
Sbjct: 8   LFLLTIFFLFLTTRRTRSS-----RLPPGPLGLPYIGQSLGLLRAMRSNSAEQWLLRRVA 67

Query: 65  KYGPVSKMTLFGKPTVFVNGAAANKAVVFSGEEGTVSSRQIESLKRILGERNLTELSGED 124
           KYGPVSKMTLFGKPTVF++G  ANKA VF+GEEG +S++Q ESLKRILGERNLTELSGED
Sbjct: 68  KYGPVSKMTLFGKPTVFIHGTVANKA-VFTGEEGAMSNQQTESLKRILGERNLTELSGED 127

Query: 125 HKRVRGALVWFLRPQSLKGFVGKMDGE----------------VSPLMKTLTFDIICSLL 184
           HKRVRGALV FL+P SLK +VGKMDGE                V PLMK LTFDIICSLL
Sbjct: 128 HKRVRGALVSFLKPDSLKKYVGKMDGEIRKHLQMYWHDNKEVTVLPLMKILTFDIICSLL 187

Query: 185 FGIEEGPTRKSIIECFKTMVDGIWSVPINLPFTRYSHSLKASAKAQQILKQLLKDKTQTM 244
           FGIEEGP RKS++ CFKTMVDGIWSVPINLPFTRYSHSLKASAK QQ+LKQLLK+K + M
Sbjct: 188 FGIEEGPRRKSMVGCFKTMVDGIWSVPINLPFTRYSHSLKASAKVQQMLKQLLKEKREKM 247

Query: 245 EEEQQEDDDKDLITYLFSIKNKDKEQALSEEEIVHNIILLMIAGHDTTSILITLMLRVLA 304
           E+E QE++ +DL+T L S+KNKDKEQALSE+EIVHNII+LM+AGHDTT+ILITLM+RVL 
Sbjct: 248 EKEDQEEEQQDLVTCLLSMKNKDKEQALSEDEIVHNIIILMVAGHDTTTILITLMIRVLG 307

Query: 305 RNPTVYAAVLQEHEEIARNKESGEALSWEDVSKMKYTWKVAMETLRLYPPVFGGFRVALK 364
            NP VYAAVLQEHEEIAR+KE GE LSWEDV KMKYTW+VA+ETLRL+PPVFGGFRVAL+
Sbjct: 308 SNPAVYAAVLQEHEEIARSKERGEPLSWEDVGKMKYTWRVALETLRLFPPVFGGFRVALR 367

Query: 365 DIQLGGYTIPKGWQIFWAAPMTHLDETIFGDPEKFEPSRFDQNQTPIPPFSFIAFGGGPR 424
           DI+ GGYTIPKGWQ+FWAAPMTHLD+TIF DP KFEP+RFD+ QT IPP  FIAFGGGPR
Sbjct: 368 DIEFGGYTIPKGWQVFWAAPMTHLDDTIFADPHKFEPNRFDK-QTLIPPLCFIAFGGGPR 427

Query: 425 ICPGYEFAKLETLVTIHYLITQFTWNLTSDDFLTRDPTLMPNKGLPIQIYPK 461
           ICPG EFAKLETL+TIHYLITQFTW L S++FLTRDPTLMPNKGLPIQIYPK
Sbjct: 428 ICPGSEFAKLETLITIHYLITQFTWKLCSEEFLTRDPTLMPNKGLPIQIYPK 472

[Close]

BLAST of Lsi04G012100 vs. NCBI nr
Match: XP_038899676.1 (taxadiene 5-alpha hydroxylase-like [Benincasa hispida])

HSP 1 Score: 823.5 bits (2126), Expect = 8.8e-235
Identity = 422/479 (88.10%), Postives = 442/479 (92.28%), Query Frame = 0


Query: 1   MITIILLLITIFLFLRFRSTFSSSEAAKNLPPGSLGVPFIGQSLSLLRAMRTNSAEQWLQ 60
           MITI LLLITIFLFLRFR+T SS+  AKNLPPG LGVPFIGQSLSLLRAMRTNSAEQWLQ
Sbjct: 1   MITIFLLLITIFLFLRFRTTSSSAATAKNLPPGPLGVPFIGQSLSLLRAMRTNSAEQWLQ 60

Query: 61  KRVAKYGPVSKMTLFGKPTVFVNGAAANKAVVFSGEEGTVSSRQIESLKRILGERNLTEL 120
           KRVAKYGPVSKMTLFGKPTVFV+GAAANKAVVF GEEGTVS+RQIESLK ILGERNLTEL
Sbjct: 61  KRVAKYGPVSKMTLFGKPTVFVHGAAANKAVVFLGEEGTVSNRQIESLKWILGERNLTEL 120

Query: 121 SGEDHKRVRGALVWFLRPQSLKGFVGKMDGE----------------VSPLMKTLTFDII 180
           SGEDHKRVRGALVWFLRPQ+LK +VGKMDGE                V+PLMKTLTFDII
Sbjct: 121 SGEDHKRVRGALVWFLRPQTLKSYVGKMDGEVRKHLNMYWHGKKEVTVAPLMKTLTFDII 180

Query: 181 CSLLFGIEEGPTRKSIIECFKTMVDGIWSVPINLPFTRYSHSLKASAKAQQILKQLLKDK 240
           CSLLFGIEEGPTRKSII+CFKTMVDGIWSVPINLPFTRY+HSLKASAKAQQILKQLLKDK
Sbjct: 181 CSLLFGIEEGPTRKSIIQCFKTMVDGIWSVPINLPFTRYNHSLKASAKAQQILKQLLKDK 240

Query: 241 TQTMEEEQQE--DDDKDLITYLFSIKNKDKEQALSEEEIVHNIILLMIAGHDTTSILITL 300
            Q MEEE++E  +DDKDLITYL SIKNKDKEQALSEEEIVHN ILLMIAGHDTTSIL+TL
Sbjct: 241 AQKMEEEEEEEKEDDKDLITYLLSIKNKDKEQALSEEEIVHNTILLMIAGHDTTSILLTL 300

Query: 301 MLRVLARNPTVYAAVLQEHEEIARNKESGEALSWEDVSKMKYTWKVAMETLRLYPPVFGG 360
           MLRVLA NPTVYAAVLQEHEEIAR+KESGEAL+WEDVSKMKYTW+VAMETLR+YPPVFGG
Sbjct: 301 MLRVLATNPTVYAAVLQEHEEIARSKESGEALTWEDVSKMKYTWRVAMETLRIYPPVFGG 360

Query: 361 FRVALKDIQLGGYTIPKGWQIFWAAPMTHLDETIFGDPEKFEPSRFDQNQTPIPPFSFIA 420
           FRVALKDIQLGGYTIPKGWQIFWAAPMTHLDETIFG+P+KFEPSRFDQNQTPIPPF FIA
Sbjct: 361 FRVALKDIQLGGYTIPKGWQIFWAAPMTHLDETIFGEPQKFEPSRFDQNQTPIPPFCFIA 420

Query: 421 FGGGPRICPGYEFAKLETLVTIHYLITQFTWNLT-SDDFLTRDPTLMPNKGLPIQIYPK 461
           FGGGPRICPG EFAKLETLVTIHYLITQFTW L+ S DFLTRDPTLMPNKGLPIQI PK
Sbjct: 421 FGGGPRICPGSEFAKLETLVTIHYLITQFTWKLSCSQDFLTRDPTLMPNKGLPIQISPK 479

[Close]

BLAST of Lsi04G012100 vs. NCBI nr
Match: XP_004152028.1 (taxadiene 5-alpha hydroxylase [Cucumis sativus] >KGN57973.1 hypothetical protein Csa_010468 [Cucumis sativus])

HSP 1 Score: 804.3 bits (2076), Expect = 5.5e-229
Identity = 411/481 (85.45%), Postives = 442/481 (91.89%), Query Frame = 0


Query: 1   MITIILLLI-TIFLFLRFRSTFSSSEAAKNLPPGSLGVPFIGQSLSLLRAMRTNSAEQWL 60
           MIT+I+LL+ TIFLF RFR+  SSS AAKNLPPG+LGVPFIGQSLSLL AMRTN+AE+WL
Sbjct: 1   MITVIILLVLTIFLFHRFRTRLSSS-AAKNLPPGALGVPFIGQSLSLLGAMRTNTAEKWL 60

Query: 61  QKRVAKYGPVSKMTLFGKPTVFVNGAAANKAVVFSGEEGTVSSRQIESLKRILGERNLTE 120
           QKRV KYGPVSKMTLFGKPTVF++GAAANKAVVFSGEEGTVS+RQ+ESLK ILG+RNLTE
Sbjct: 61  QKRVDKYGPVSKMTLFGKPTVFIHGAAANKAVVFSGEEGTVSNRQVESLKMILGKRNLTE 120

Query: 121 LSGEDHKRVRGALVWFLRPQSLKGFVGKMDGE----------------VSPLMKTLTFDI 180
           LSGEDHKRVRGALVWFLRPQ+L+ +VGKMDGE                V+PLMKTLTFDI
Sbjct: 121 LSGEDHKRVRGALVWFLRPQTLRSYVGKMDGEVRRHLNMYWHGNNEVTVAPLMKTLTFDI 180

Query: 181 ICSLLFGIEEGPTRKSIIECFKTMVDGIWSVPINLPFTRYSHSLKASAKAQQILKQLLKD 240
           ICSLLFGIEEG TRKSIIECFKTMVDGIWS+PINLPFTRY+HSLKASAKAQQILKQLLKD
Sbjct: 181 ICSLLFGIEEGTTRKSIIECFKTMVDGIWSIPINLPFTRYNHSLKASAKAQQILKQLLKD 240

Query: 241 KTQTMEEEQQ---EDDDKDLITYLFSIKNKDKEQALSEEEIVHNIILLMIAGHDTTSILI 300
           K + MEEE++   E++DKDLI+YL  IKNKDKEQALSEEEIVHNIILLMIAGHDTTSIL+
Sbjct: 241 KAKVMEEEKESEVEENDKDLISYLLRIKNKDKEQALSEEEIVHNIILLMIAGHDTTSILL 300

Query: 301 TLMLRVLARNPTVYAAVLQEHEEIARNKESGEALSWEDVSKMKYTWKVAMETLRLYPPVF 360
           TLMLRVLA NPTVYAAVLQEHEEI R+KE GEAL+WEDVSKMKYTW+VAMETLRLYPPVF
Sbjct: 301 TLMLRVLATNPTVYAAVLQEHEEIGRSKERGEALTWEDVSKMKYTWRVAMETLRLYPPVF 360

Query: 361 GGFRVALKDIQLGGYTIPKGWQIFWAAPMTHLDETIFGDPEKFEPSRFDQNQTPIPPFSF 420
           GGFRVALKDIQLG YTIPKGWQIFWAAPMTHLDETIFGDP+KFEPSRFDQNQTPIPPFSF
Sbjct: 361 GGFRVALKDIQLGAYTIPKGWQIFWAAPMTHLDETIFGDPQKFEPSRFDQNQTPIPPFSF 420

Query: 421 IAFGGGPRICPGYEFAKLETLVTIHYLITQFTWNLT-SDDFLTRDPTLMPNKGLPIQIYP 461
           IAFGGGPRICPGYEFAKLETLVTIHYLITQFTWNL+ S DFLTRDPTLMPNKGLPIQI+P
Sbjct: 421 IAFGGGPRICPGYEFAKLETLVTIHYLITQFTWNLSCSQDFLTRDPTLMPNKGLPIQIFP 480

[Close]

BLAST of Lsi04G012100 vs. NCBI nr
Match: XP_023535435.1 (taxadiene 5-alpha hydroxylase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 795.4 bits (2053), Expect = 2.6e-226
Identity = 405/478 (84.73%), Postives = 435/478 (91.00%), Query Frame = 0


Query: 1   MITIILLLITIFLFLRFRSTFSSSEAAKNLPPGSLGVPFIGQSLSLLRAMRTNSAEQWLQ 60
           MI IILLLITIFL+LR R+TF S+ AAKNLPPG LGVP+IGQSLSLLRAMR NSAEQWLQ
Sbjct: 1   MIAIILLLITIFLYLRLRTTFFSA-AAKNLPPGRLGVPYIGQSLSLLRAMRANSAEQWLQ 60

Query: 61  KRVAKYGPVSKMTLFGKPTVFVNGAAANKAVVFSGEEGTVSSRQIESLKRILGERNLTEL 120
           +RVAKYGP+SKMTLFGKPTVFV+G AANKAVVF GEE  VS+RQ ESL+RILGERNLTEL
Sbjct: 61  QRVAKYGPISKMTLFGKPTVFVHGVAANKAVVFLGEEEAVSNRQTESLRRILGERNLTEL 120

Query: 121 SGEDHKRVRGALVWFLRPQSLKGFVGKMDGE----------------VSPLMKTLTFDII 180
           SGEDHKRVRGALVWFLRPQSLKGFVGKMDGE                V+PLMKTLTFDII
Sbjct: 121 SGEDHKRVRGALVWFLRPQSLKGFVGKMDGEVRKHLNLHWHGNKEVIVAPLMKTLTFDII 180

Query: 181 CSLLFGIEEGPTRKSIIECFKTMVDGIWSVPINLPFTRYSHSLKASAKAQQILKQLLKDK 240
           CSLLFGIEEGPTRKSIIECFKTMVDGIWSVPINLPFTRYSHSLKASAK QQILKQLLKDK
Sbjct: 181 CSLLFGIEEGPTRKSIIECFKTMVDGIWSVPINLPFTRYSHSLKASAKVQQILKQLLKDK 240

Query: 241 TQTMEEEQQEDDDKDLITYLFSIKNKDKEQALSEEEIVHNIILLMIAGHDTTSILITLML 300
            Q MEEE+++++DK+LIT L SIKNKDKEQALSEEEIVHNIILL+IAGHDTTSILITLML
Sbjct: 241 AQKMEEEEEKEEDKNLITCLLSIKNKDKEQALSEEEIVHNIILLLIAGHDTTSILITLML 300

Query: 301 RVLARNPTVYAAVLQEHEEIARNKESGEALSWEDVSKMKYTWKVAMETLRLYPPVFGGFR 360
           RV+A NPTVYAAVLQEHE+I R+KE GEAL+WEDVSKMKYTW+VA+ETLRL+PPVFGGFR
Sbjct: 301 RVMATNPTVYAAVLQEHEDILRSKECGEALTWEDVSKMKYTWRVALETLRLFPPVFGGFR 360

Query: 361 VALKDIQLGGYTIPKGWQIFWAAPMTHLDETIFGDPEKFEPSRFDQNQTPI-PPFSFIAF 420
           +ALKDIQLGGYTIPKGWQIFWAA MTHLDE IF DP+KFEPSRFDQNQT I PP SFIAF
Sbjct: 361 MALKDIQLGGYTIPKGWQIFWAASMTHLDEKIFRDPQKFEPSRFDQNQTSIPPPLSFIAF 420

Query: 421 GGGPRICPGYEFAKLETLVTIHYLITQFTWNLTSDDFLTRDPTLMPNKGLPIQIYPKL 462
           GGGPRICPGYEFAKLETLVTIHY+ITQFTW L+S DFLTRDP+LMPNKGLPIQ+YPKL
Sbjct: 421 GGGPRICPGYEFAKLETLVTIHYIITQFTWKLSSQDFLTRDPSLMPNKGLPIQLYPKL 477

[Close]

BLAST of Lsi04G012100 vs. NCBI nr
Match: XP_022976494.1 (taxadiene 5-alpha hydroxylase-like [Cucurbita maxima])

HSP 1 Score: 795.0 bits (2052), Expect = 3.4e-226
Identity = 406/479 (84.76%), Postives = 433/479 (90.40%), Query Frame = 0


Query: 1   MITIILLLITIFLFLRFRSTFSSSEAAKNLPPGSLGVPFIGQSLSLLRAMRTNSAEQWLQ 60
           MI  ILLLITIFL+LR R+TF SS  AKNLPPG LGVPFIGQSLSLLRAMR NSAEQWLQ
Sbjct: 1   MIATILLLITIFLYLRLRATFFSSAEAKNLPPGPLGVPFIGQSLSLLRAMRANSAEQWLQ 60

Query: 61  KRVAKYGPVSKMTLFGKPTVFVNGAAANKAVVFSGEEGTVSSRQIESLKRILGERNLTEL 120
           +RVAKYGP+SKMTLFGKPTVFV+G AANKAVVFSGEE  VS+RQ ESL+RILGERNLTEL
Sbjct: 61  QRVAKYGPISKMTLFGKPTVFVHGVAANKAVVFSGEEEAVSNRQTESLRRILGERNLTEL 120

Query: 121 SGEDHKRVRGALVWFLRPQSLKGFVGKMDGE----------------VSPLMKTLTFDII 180
           SGEDHKRVRGALVWFLRPQSLKGFVGKMDGE                V+PLMKTLTFDII
Sbjct: 121 SGEDHKRVRGALVWFLRPQSLKGFVGKMDGEVRKHLNLHWHGNKEVIVAPLMKTLTFDII 180

Query: 181 CSLLFGIEEGPTRKSIIECFKTMVDGIWSVPINLPFTRYSHSLKASAKAQQILKQLLKDK 240
           CSLLFGIEEGPTRKSIIECFKTMVDGIWSVPINLPFTRYSHSL ASAK QQILKQLLKDK
Sbjct: 181 CSLLFGIEEGPTRKSIIECFKTMVDGIWSVPINLPFTRYSHSLNASAKVQQILKQLLKDK 240

Query: 241 TQTMEEEQQE-DDDKDLITYLFSIKNKDKEQALSEEEIVHNIILLMIAGHDTTSILITLM 300
            Q MEEE++E ++DK+LIT L SIKNKDKEQALSEEEIVHNIILL+IAGHDTTSILITLM
Sbjct: 241 AQKMEEEEEEKEEDKNLITCLLSIKNKDKEQALSEEEIVHNIILLLIAGHDTTSILITLM 300

Query: 301 LRVLARNPTVYAAVLQEHEEIARNKESGEALSWEDVSKMKYTWKVAMETLRLYPPVFGGF 360
           LRV+A NPTVYAAVLQEHE+I R+KE GEAL+WEDVSKMKYTW+VA+ETLRL+PPVFGGF
Sbjct: 301 LRVMATNPTVYAAVLQEHEDIHRSKECGEALTWEDVSKMKYTWRVALETLRLFPPVFGGF 360

Query: 361 RVALKDIQLGGYTIPKGWQIFWAAPMTHLDETIFGDPEKFEPSRFDQNQTPI-PPFSFIA 420
           RVALKDIQLGGYTIPKGWQIFWAA MTHLDE IF DP+KF+PSRFDQNQT I PP SFIA
Sbjct: 361 RVALKDIQLGGYTIPKGWQIFWAASMTHLDEKIFTDPQKFKPSRFDQNQTSIPPPLSFIA 420

Query: 421 FGGGPRICPGYEFAKLETLVTIHYLITQFTWNLTSDDFLTRDPTLMPNKGLPIQIYPKL 462
           FGGGPRICPGYEFAKLETLVTIHY+ITQFTW L+S DFLTRDP+LMPNKGLPIQ+YPKL
Sbjct: 421 FGGGPRICPGYEFAKLETLVTIHYIITQFTWKLSSQDFLTRDPSLMPNKGLPIQLYPKL 479

[Close]

BLAST of Lsi04G012100 vs. NCBI nr
Match: XP_022936436.1 (taxadiene 5-alpha hydroxylase-like [Cucurbita moschata])

HSP 1 Score: 793.9 bits (2049), Expect = 7.5e-226
Identity = 406/480 (84.58%), Postives = 437/480 (91.04%), Query Frame = 0


Query: 1   MITIILLLITIFLFLRFRST-FSSSEAAKNLPPGSLGVPFIGQSLSLLRAMRTNSAEQWL 60
           MI IILLLITIF++LR R+T FS++ AAKNLPPG LGVP+IGQSLSLLRAMR NSAEQWL
Sbjct: 1   MIAIILLLITIFVYLRLRTTFFSTAVAAKNLPPGRLGVPYIGQSLSLLRAMRANSAEQWL 60

Query: 61  QKRVAKYGPVSKMTLFGKPTVFVNGAAANKAVVFSGEEGTVSSRQIESLKRILGERNLTE 120
           Q+RVAKYGP+SKMTLFGKPTVFV+G AANKAVVFSGEE  VS+RQ ESL+RILGERNLTE
Sbjct: 61  QQRVAKYGPISKMTLFGKPTVFVHGVAANKAVVFSGEEEAVSNRQTESLRRILGERNLTE 120

Query: 121 LSGEDHKRVRGALVWFLRPQSLKGFVGKMDGE----------------VSPLMKTLTFDI 180
           LSGEDHKRVRGALVWFLRPQSLKGFVGKMDGE                V+PLMKTLTFDI
Sbjct: 121 LSGEDHKRVRGALVWFLRPQSLKGFVGKMDGEVRKHLKLHWHGNKEVIVAPLMKTLTFDI 180

Query: 181 ICSLLFGIEEGPTRKSIIECFKTMVDGIWSVPINLPFTRYSHSLKASAKAQQILKQLLKD 240
           ICSLLFGIEEGPTRKSIIECFKTMVDGIWSVPINLPFTRYSHSLKASAK QQILKQLLKD
Sbjct: 181 ICSLLFGIEEGPTRKSIIECFKTMVDGIWSVPINLPFTRYSHSLKASAKVQQILKQLLKD 240

Query: 241 KTQTME-EEQQEDDDKDLITYLFSIKNKDKEQALSEEEIVHNIILLMIAGHDTTSILITL 300
           K Q ME EE+++++DK+LIT L SIKNKDKEQALSEEEIVHNIILL+IAGHDTTSILITL
Sbjct: 241 KAQKMEGEEEEKEEDKNLITCLLSIKNKDKEQALSEEEIVHNIILLLIAGHDTTSILITL 300

Query: 301 MLRVLARNPTVYAAVLQEHEEIARNKESGEALSWEDVSKMKYTWKVAMETLRLYPPVFGG 360
           MLRV+A NPTVYAAVLQEHE+I R+KE GEAL+WEDVSKMKYTW+VA+ETLRL+PPVFGG
Sbjct: 301 MLRVMATNPTVYAAVLQEHEDILRSKECGEALTWEDVSKMKYTWRVALETLRLFPPVFGG 360

Query: 361 FRVALKDIQLGGYTIPKGWQIFWAAPMTHLDETIFGDPEKFEPSRFDQNQTPI-PPFSFI 420
           FRVALKDIQLGGYTIPKGWQIFWAA MTHLDE IF DP+KFEPSRFDQNQT I PP SFI
Sbjct: 361 FRVALKDIQLGGYTIPKGWQIFWAASMTHLDEKIFRDPQKFEPSRFDQNQTSIPPPLSFI 420

Query: 421 AFGGGPRICPGYEFAKLETLVTIHYLITQFTWNLTSDDFLTRDPTLMPNKGLPIQIYPKL 462
           AFGGGPRICPGYEFAKLETLVTIHY+ITQFTW L+S DFLTRDP+LMPNKGLPIQ+YPKL
Sbjct: 421 AFGGGPRICPGYEFAKLETLVTIHYIITQFTWKLSSQDFLTRDPSLMPNKGLPIQLYPKL 480

[Close]

BLAST of Lsi04G012100 vs. TAIR 10
Match: AT2G42850.1 (cytochrome P450, family 718 )

HSP 1 Score: 303.5 bits (776), Expect = 2.9e-82
Identity = 166/472 (35.17%), Postives = 275/472 (58.26%), Query Frame = 0


Query: 10  TIFLFLRFRSTFSSS--EAAKNLPPGSLGVPFIGQSLSLLRAMRTNSA-EQWLQKRVAKY 69
           T+F F +    F +   +  K L PG +G+P+IG+++   +A ++N   E ++  R+ K+
Sbjct: 23  TLFFFRKKHHRFITKKIQKKKKLLPGEMGLPWIGETMDFYKAQKSNRVFEDFVNPRIIKH 82

Query: 70  GPVSKMTLFGKPTVFVNGAAANKAVVFSGEEGTVSSRQIESLKRILGERNLTELSGEDHK 129
           G + K  + G PT+ VNGA AN+ ++ S E   V S    S  +++G   +    GE H+
Sbjct: 83  GNIFKTRIMGSPTIVVNGAEANR-LILSNEFSLVVSSWPSSSVQLMGMNCIMAKQGEKHR 142

Query: 130 RVRGALVWFLRPQSLKGFV----------------GKMDGEVSPLMKTLTFDIICSLLFG 189
            +RG +   L    L+  +                GK +  +    K LTF ++   L+G
Sbjct: 143 VLRGIVANSLSYIGLESLIPKLCDTVKFHHETEWRGKEEISLYRSAKVLTFTVVFECLYG 202

Query: 190 IEEGPTRKSIIECFKTMVDGIWSVPINLPFTRYSHSLKASAKAQQILKQLLKDKTQTMEE 249
           I+       ++E F+ +++G++++P+  P ++++ + KA  + +  L   +++K + ME+
Sbjct: 203 IK---VEIGMLEVFERVLEGVFALPVEFPCSKFARAKKARLEIETFLVGKVREKRREMEK 262

Query: 250 EQQEDDDKDLITYLFSIKNKDKEQALSEEEIVHNIILLMIAGHDTTSILITLMLRVLARN 309
           E  E  +  L + L     K     ++EEE+V N++LL+ A HDTTS  +++  ++LA++
Sbjct: 263 EGAEKPNTTLFSRLVEELIKG---VITEEEVVDNMVLLVFAAHDTTSYAMSMTFKMLAQH 322

Query: 310 PTVYAAVLQEHEEIARNKESGEALSWEDVSKMKYTWKVAMETLRLYPPVFGGFRVALKDI 369
           PT    +LQEH +I  NK  GE L+ EDV KMKY+W+V  ET+RL PP+FG FR A+ DI
Sbjct: 323 PTCRDTLLQEHAQIKANKGEGEYLTVEDVKKMKYSWQVVRETMRLSPPIFGSFRKAVADI 382

Query: 370 QLGGYTIPKGWQIFWAAPMTHLDETIFGDPEKFEPSRFDQNQTPIPPFSFIAFGGGPRIC 429
             GGYTIPKGW+I W    TH +  IF DP  F+P+RFD+   PI  ++++ FGGGPR+C
Sbjct: 383 DYGGYTIPKGWKILWTTYGTHYNPEIFQDPMSFDPTRFDK---PIQAYTYLPFGGGPRLC 442

Query: 430 PGYEFAKLETLVTIHYLITQFTWNLT-SDDFLTRDPTLMPNKGLPIQIYPKL 462
            G++ AK+  LV +H+++T F W+L   D+ ++ DP   P+ G+PI+I PK+
Sbjct: 443 AGHQLAKISILVFMHFVVTGFDWSLVYPDETISMDPLPFPSLGMPIKISPKV 484

[Close]

BLAST of Lsi04G012100 vs. TAIR 10
Match: AT5G36110.1 (cytochrome P450, family 716, subfamily A, polypeptide 1 )

HSP 1 Score: 295.4 bits (755), Expect = 7.8e-80
Identity = 171/482 (35.48%), Postives = 278/482 (57.68%), Query Frame = 0


Query: 1   MITIILLLITIFLFLRFRSTFSSSEAAKNLPPGSLGVPFIGQSLSLLRAMRTNSAEQWLQ 60
           MI + L  I + L L  R   S   +  NLPPG+ G+P IG+S S L A R    E+++ 
Sbjct: 6   MIILFLSSILLSLLLLLRKHLSHF-SYPNLPPGNTGLPLIGESFSFLSAGRQGHPEKFIT 65

Query: 61  KRVAKYGP----VSKMTLFGKPTVFVNGAAANKAVVFSGEEGTVSSRQIESLKRILGERN 120
            RV ++      V K  LFG PT  V GA+ NK  +F+ E   V S   +S+ +I     
Sbjct: 66  DRVRRFSSSSSCVFKTHLFGSPTAVVTGASGNK-FLFTNENKLVVSWWPDSVNKIFPSSM 125

Query: 121 LTELSGEDHKRVRGALVWFLRPQSLKGFVGKMDG----------------EVSPLMKTLT 180
            T  S E+ +++R  L  F++P++L+ +VG MD                  V PL K  T
Sbjct: 126 QTS-SKEEARKLRMLLSQFMKPEALRRYVGVMDEIAQRHFETEWANQDQVIVFPLTKKFT 185

Query: 181 FDIICSLLFGIEEGPTRKSIIECFKTMVDGIWSVPINLPFTRYSHSLKASAKAQQILKQL 240
           F I C     +E+    + + E F T+  GI+S+PI+LP TR++ ++KAS   ++ +  +
Sbjct: 186 FSIACRSFLSMEDPARVRQLEEQFNTVAVGIFSIPIDLPGTRFNRAIKASRLLRKEVSAI 245

Query: 241 LKDKTQTMEEEQQEDDDKDLITYLFSIKNKDKEQALSEEEIVHNIILLMIAGHDTTSILI 300
           ++ + + ++  +   ++ D+++++     + K     +E++   II L+I GHDT SI+ 
Sbjct: 246 VRQRKEELKAGKAL-EEHDILSHMLMNIGETK-----DEDLADKIIGLLIGGHDTASIVC 305

Query: 301 TLMLRVLARNPTVYAAVLQEHEEIARNKESGEALSWEDVSKMKYTWKVAMETLRLYPPVF 360
           T ++  LA  P VY  VLQE +EI + K+  E L WED+ KM+Y+W VA E +R+ PP+ 
Sbjct: 306 TFVVNYLAEFPHVYQRVLQEQKEILKEKKEKEGLRWEDIEKMRYSWNVACEVMRIVPPLS 365

Query: 361 GGFRVALKDIQLGGYTIPKGWQIFWAAPMTHLDETIFGDPEKFEPSRFDQNQTPIPPFSF 420
           G FR A+      G+ IPKGW+++W+A  TH++   F +PE+FEP+RF+ +     P+++
Sbjct: 366 GTFREAIDHFSFKGFYIPKGWKLYWSATATHMNPDYFPEPERFEPNRFEGSGP--KPYTY 425

Query: 421 IAFGGGPRICPGYEFAKLETLVTIHYLITQFTWN--LTSDDFLTRDPTLMPNKGLPIQIY 461
           + FGGGPR+CPG E+A+LE L+ +H L+ +F W     +++ +  DP  +P+KGLPI+I+
Sbjct: 426 VPFGGGPRMCPGKEYARLEILIFMHNLVNRFKWEKVFPNENKIVVDPLPIPDKGLPIRIF 476

[Close]

BLAST of Lsi04G012100 vs. TAIR 10
Match: AT5G05690.1 (Cytochrome P450 superfamily protein )

HSP 1 Score: 233.4 bits (594), Expect = 3.6e-61
Identity = 150/469 (31.98%), Postives = 240/469 (51.17%), Query Frame = 0


Query: 5   ILLLITI---FLFLRFRSTFSSSEAAKNLPPGSLGVPFIGQSLSLLRAMRTNSAEQWLQK 64
           +LLL +I   FL L  R+ +        LPPGSLG+P IG++  L+ A +T + E ++ +
Sbjct: 7   LLLLSSIAAGFLLLLRRTRY----RRMGLPPGSLGLPLIGETFQLIGAYKTENPEPFIDE 66

Query: 65  RVAKYGPVSKMTLFGKPTVFVNGAAANKAVVFSGEEGTVSSRQIESLKRILGERNLTELS 124
           RVA+YG V    LFG+PT+F      N+  V   E          S+  +LG+ +L  + 
Sbjct: 67  RVARYGSVFMTHLFGEPTIFSADPETNR-FVLQNEGKLFECSYPASICNLLGKHSLLLMK 126

Query: 125 GEDHKRVRGALVWFLRPQSLKGFV---------GKMDGEVSPLM-----KTLTFDIICSL 184
           G  HKR+    + F     +K  +           +D   S ++     K +TF++    
Sbjct: 127 GSLHKRMHSLTMSFANSSIIKDHLMLDIDRLVRFNLDSWSSRVLLMEEAKKITFELTVKQ 186

Query: 185 LFGIEEGPTRKSIIECFKTMVDGIWSVPINLPFTRYSHSLKASAKAQQILKQLLKDKTQT 244
           L   + G   +S+ + +  +++G +S+P+ L  T Y  +++A  K  + L  ++  + + 
Sbjct: 187 LMSFDPGEWSESLRKEYLLVIEGFFSLPLPLFSTTYRKAIQARRKVAEALTVVVMKRRE- 246

Query: 245 MEEEQQEDDDKDLITYLFSIKNKDKEQALSEEEIVHNIILLMIAGHDTTSILITLMLRVL 304
            EEE+  +  KD++  L +      +   S+EEIV  ++ L++AG++TTS ++TL ++ L
Sbjct: 247 -EEEEGAERKKDMLAALLA-----ADDGFSDEEIVDFLVALLVAGYETTSTIMTLAVKFL 306

Query: 305 ARNPTVYAAVLQEHEEIARNKESGEALSWEDVSKMKYTWKVAMETLRLYPPVFGGFRVAL 364
              P   A + +EHE+I   K    +L W D   M +T  V  ETLR+   + G FR A+
Sbjct: 307 TETPLALAQLKEEHEKIRAMKSDSYSLEWSDYKSMPFTQCVVNETLRVANIIGGVFRRAM 366

Query: 365 KDIQLGGYTIPKGWQIFWAAPMTHLDETIFGDPEKFEPSRFDQNQTPIPPFS-FIAFGGG 424
            D+++ GY IPKGW++F +    HLD   F D   F P R+  N     P + F  FGGG
Sbjct: 367 TDVEIKGYKIPKGWKVFSSFRAVHLDPNHFKDARTFNPWRWQSNSVTTGPSNVFTPFGGG 426

Query: 425 PRICPGYEFAKLETLVTIHYLITQFTWNLTSDDFLTRDPTLMPNKGLPI 456
           PR+CPGYE A++   V +H L+T F+W     D L   PT    K  PI
Sbjct: 427 PRLCPGYELARVALSVFLHRLVTGFSWVPAEQDKLVFFPTTRTQKRYPI 463

[Close]

BLAST of Lsi04G012100 vs. TAIR 10
Match: AT3G13730.1 (cytochrome P450, family 90, subfamily D, polypeptide 1 )

HSP 1 Score: 225.7 bits (574), Expect = 7.6e-59
Identity = 139/454 (30.62%), Postives = 240/454 (52.86%), Query Frame = 0


Query: 22  SSSEAAKNLPPGSLGVPFIGQSLSLLRAMRTNSAEQWLQKRVAKYGPVSKMTLFGKPTVF 81
           +S       P GSLG P IG+++  + +  ++  E ++ KR   YG V K  +FG  T+ 
Sbjct: 43  TSQSHGPKFPHGSLGWPVIGETIEFVSSAYSDRPESFMDKRRLMYGRVFKSHIFGTATIV 102

Query: 82  VNGAAANKAVVFSGEEGTVSSRQIESLKRILGERNLTELSGEDHKRVRGALVWFLRPQSL 141
              A  N+AV+ S     V     ++++ ++G+ ++  ++G  H+R  G +  FL+   L
Sbjct: 103 STDAEVNRAVLQSDSTAFVPFYP-KTVRELMGKSSILLINGSLHRRFHGLVGSFLKSPLL 162

Query: 142 KGFVGK---------MD--GEVSPLM-----KTLTFDIICSLLFGIEEGPTRKSIIECFK 201
           K  + +         MD   E  P++     KT+ F ++   L  +E+G   + +   F+
Sbjct: 163 KAQIVRDMHKFLSESMDLWSEDQPVLLQDVSKTVAFKVLAKALISVEKGEDLEELKREFE 222

Query: 202 TMVDGIWSVPINLPFTRYSHSLKASAKAQQILKQLLKDKTQTMEEEQQED-DDKDLITYL 261
             + G+ S+PIN P T+   SL+A     + ++++++ K +  + ++++D   KD++  L
Sbjct: 223 NFISGLMSLPINFPGTQLHRSLQAKKNMVKQVERIIEGKIRKTKNKEEDDVIAKDVVDVL 282

Query: 262 FSIKNKDKEQALSEEEIVHNIILLMIAGHDTTSILITLMLRVLARNPTVYAAVLQEHEEI 321
                KD  + L+   I +N+I +MI GHD+  +LITL ++ L+ +P     + +E+ ++
Sbjct: 283 L----KDSSEHLTHNLIANNMIDMMIPGHDSVPVLITLAVKFLSDSPAALNLLTEENMKL 342

Query: 322 ARNKE-SGEALSWEDVSKMKYTWKVAMETLRLYPPVFGGFRVALKDIQLGGYTIPKGWQI 381
              KE +GE L W D   + +T KV  ETLR+   + G  R A+KD+++ GY IPKGW  
Sbjct: 343 KSLKELTGEPLYWNDYLSLPFTQKVITETLRMGNVIIGVMRKAMKDVEIKGYVIPKGWCF 402

Query: 382 FWAAPMTHLDETIFGDPEKFEPSRFDQNQTPIPPFSFIAFGGGPRICPGYEFAKLETLVT 441
                  HLD+  +  P KF P R+ +    +   SF  FGGG R+CPG + A+LET V 
Sbjct: 403 LAYLRSVHLDKLYYESPYKFNPWRWQERD--MNTSSFSPFGGGQRLCPGLDLARLETSVF 462

Query: 442 IHYLITQFTWNLTSDDFLTRDPTLMPNKGLPIQI 458
           +H+L+T+F W +  +D +   PT+     LPI I
Sbjct: 463 LHHLVTRFRW-IAEEDTIINFPTVHMKNKLPIWI 488

[Close]

BLAST of Lsi04G012100 vs. TAIR 10
Match: AT4G19230.1 (cytochrome P450, family 707, subfamily A, polypeptide 1 )

HSP 1 Score: 220.3 bits (560), Expect = 3.2e-57
Identity = 153/481 (31.81%), Postives = 246/481 (51.14%), Query Frame = 0


Query: 4   IILLLITIF---LFLRF------RSTFSSSEAAKNLPPGSLGVPFIGQSLSLLRAMRTNS 63
           I  L +T+F   LFL F      +  F SS+    LPPG++G P++G++  L     +  
Sbjct: 3   ISALFLTLFAGSLFLYFLRCLISQRRFGSSKLP--LPPGTMGWPYVGETFQLY----SQD 62

Query: 64  AEQWLQKRVAKYGPVSKMTLFGKPTVFVNGAAANKAVVFSGEEGTVSSRQIESLKRILGE 123
              + Q +  +YG V K  + G P V ++   A K V+ + +          S +R+LG+
Sbjct: 63  PNVFFQSKQKRYGSVFKTHVLGCPCVMISSPEAAKFVLVT-KSHLFKPTFPASKERMLGK 122

Query: 124 RNLTELSGEDHKRVRGALVWFLRPQSLKGFVGKMDG--------------EVSPLMKTLT 183
           + +    G+ H ++R  ++    P+S++  V  ++                    MKT T
Sbjct: 123 QAIFFHQGDYHAKLRKLVLRAFMPESIRNMVPDIESIAQDSLRSWEGTMINTYQEMKTYT 182

Query: 184 FDIICSLLFGIEEGPTRKSIIECFKTMVDGIWSVPINLPFTRYSHSLKASAKAQQILKQL 243
           F++    +FG +E   R+ +  C+  +  G  S+P+NLP T +  S+KA  +  QIL ++
Sbjct: 183 FNVALLSIFGKDEVLYREDLKRCYYILEKGYNSMPVNLPGTLFHKSMKARKELSQILARI 242

Query: 244 LKDKTQTMEEEQQEDDDKDLITYLFSIKNKDKEQALSEEEIVHNIILLMIAGHDTTSILI 303
           L       E  Q      DL+         DKE+ L++E+I  NII ++ A  DTT+ ++
Sbjct: 243 LS------ERRQNGSSHNDLLGSFMG----DKEE-LTDEQIADNIIGVIFAARDTTASVM 302

Query: 304 TLMLRVLARNPTVYAAVLQEHEEIARNKESGEALSWEDVSKMKYTWKVAMETLRLYPPVF 363
           + +L+ LA NP V  AV +E   I ++KE GE+L+W D  KM  T +V  ETLR+   + 
Sbjct: 303 SWILKYLAENPNVLEAVTEEQMAIRKDKEEGESLTWGDTKKMPLTSRVIQETLRVASILS 362

Query: 364 GGFRVALKDIQLGGYTIPKGWQIFWAAPMTHLDETIFGDPEKFEPSRFDQNQTPIPPFSF 423
             FR A++D++  GY IPKGW++       H    IF +P KF+PSRF   +    P +F
Sbjct: 363 FTFREAVEDVEYEGYLIPKGWKVLPLFRNIHHSADIFSNPGKFDPSRF---EVAPKPNTF 422

Query: 424 IAFGGGPRICPGYEFAKLETLVTIHYLITQFTWNLT-SDDFLTRDPTLMPNKGLPIQIYP 461
           + FG G   CPG E AKLE  + IH+L T+++W++  + D +   P  +P  GLPI +  
Sbjct: 423 MPFGNGTHSCPGNELAKLEMSIMIHHLTTKYSWSIVGASDGIQYGPFALPQNGLPIVLAR 462

The following BLAST results are available for this feature:

BLAST of Lsi04G012100 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q6WG30	2.0e-88	38.17	Taxadiene 5-alpha hydroxylase OS=Taxus cuspidata OX=99806 PE=1 SV=2	[more]
Q9AXM6	1.7e-84	36.23	Taxane 10-beta-hydroxylase OS=Taxus cuspidata OX=99806 GN=CYP725A1 PE=1 SV=1	[more]
Q84KI1	1.1e-83	36.15	Taxoid 14-beta-hydroxylase OS=Taxus cuspidata OX=99806 PE=1 SV=1	[more]
Q50EK1	8.9e-81	36.48	Cytochrome P450 716B1 OS=Picea sitchensis OX=3332 GN=CYP716B1 PE=2 SV=1	[more]
Q50EK0	1.7e-79	36.69	Cytochrome P450 716B2 OS=Picea sitchensis OX=3332 GN=CYP716B2 PE=2 SV=1	[more]

BLAST of Lsi04G012100 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L864	2.7e-229	85.45	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G416660 PE=3 SV=1	[more]
A0A6J1INM7	1.6e-226	84.76	taxadiene 5-alpha hydroxylase-like OS=Cucurbita maxima OX=3661 GN=LOC111476875 P...	[more]
A0A6J1F7G6	3.6e-226	84.58	taxadiene 5-alpha hydroxylase-like OS=Cucurbita moschata OX=3662 GN=LOC111443055...	[more]
A0A1S3C037	3.5e-221	84.13	LOW QUALITY PROTEIN: taxadiene 5-alpha hydroxylase-like OS=Cucumis melo OX=3656 ...	[more]
A0A6J1CCZ3	1.3e-202	76.69	taxadiene 5-alpha hydroxylase-like OS=Momordica charantia OX=3673 GN=LOC11101035...	[more]

BLAST of Lsi04G012100 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038899676.1	8.8e-235	88.10	taxadiene 5-alpha hydroxylase-like [Benincasa hispida]	[more]
XP_004152028.1	5.5e-229	85.45	taxadiene 5-alpha hydroxylase [Cucumis sativus] >KGN57973.1 hypothetical protein...	[more]
XP_023535435.1	2.6e-226	84.73	taxadiene 5-alpha hydroxylase-like [Cucurbita pepo subsp. pepo]	[more]
XP_022976494.1	3.4e-226	84.76	taxadiene 5-alpha hydroxylase-like [Cucurbita maxima]	[more]
XP_022936436.1	7.5e-226	84.58	taxadiene 5-alpha hydroxylase-like [Cucurbita moschata]	[more]

BLAST of Lsi04G012100 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G42850.1	2.9e-82	35.17	cytochrome P450, family 718	[more]
AT5G36110.1	7.8e-80	35.48	cytochrome P450, family 716, subfamily A, polypeptide 1	[more]
AT5G05690.1	3.6e-61	31.98	Cytochrome P450 superfamily protein	[more]
AT3G13730.1	7.6e-59	30.62	cytochrome P450, family 90, subfamily D, polypeptide 1	[more]
AT4G19230.1	3.2e-57	31.81	cytochrome P450, family 707, subfamily A, polypeptide 1	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 214..234
None	No IPR available	PANTHER	PTHR24286	CYTOCHROME P450 26	coord: 5..458
None	No IPR available	PANTHER	PTHR24286:SF237	OS07G0520300 PROTEIN	coord: 5..458
IPR001128	Cytochrome P450	PRINTS	PR00385	P450	coord: 327..338 score: 41.42 coord: 401..410 score: 54.2 coord: 410..421 score: 36.51 coord: 271..288 score: 35.25
IPR001128	Cytochrome P450	PFAM	PF00067	p450	coord: 150..450 e-value: 5.3E-66 score: 223.2
IPR002401	Cytochrome P450, E-class, group I	PRINTS	PR00463	EP450I	coord: 366..390 score: 28.91 coord: 400..410 score: 49.4 coord: 260..277 score: 31.59 coord: 410..433 score: 25.19 coord: 63..82 score: 28.82 coord: 280..306 score: 19.48
IPR036396	Cytochrome P450 superfamily	GENE3D	1.10.630.10	Cytochrome P450	coord: 20..459 e-value: 6.8E-118 score: 396.2
IPR036396	Cytochrome P450 superfamily	SUPERFAMILY	48264	Cytochrome P450	coord: 28..459
IPR017972	Cytochrome P450, conserved site	PROSITE	PS00086	CYTOCHROME_P450	coord: 403..412

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi04G012100.1	Lsi04G012100.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016125	sterol metabolic process
cellular_component	GO:0016020	membrane
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0004497	monooxygenase activity
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen