Lsi04G011630 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi04G011630
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionNudix hydrolase
Locationchr04: 16797077 .. 16798953 (-)
RNA-Seq ExpressionLsi04G011630
SyntenyLsi04G011630
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCAGGTTTTTGTGGCTTCTAGACTGAATGTTCCAGGAGCATGGCAGATGCCTCAGGTGATTTTGAAATTTCCTCTGTTCTTTTCCTCTGCTTCATTTTGGAATTTTGTTTCATTGCTCAATATATCTAAATGGCTCATTTTTAGTTCATGGGAAGATGTTGTTCCAAATTCTGTTCAATTGTATGAATTTTGGAGACTGCAGGTTAGTTGTGAACTTGTGAACTGTAATGGATTATCTGCCCTTTTGATATCGACGGCGTTTGAATCGGTATCGCTATAAGTTGGCTCATGATGAAAGAGGGAAGCTATAAACCTATAACAACATGTCATAGCTGATTTGGGATATGTTGTTGAAATAAACAGAAAAGTTTCGAATCAATATCTCAAATACTATCGAGATCCAAGGACCGTTATTGTGGTTCTAAAGAACAGTGGTTGCATCGATATAACGACACGATGTAATGGTATATTGAGTGTAACAGGATCTCGCTATATGCTCTTGCCTCTCATCATTGCTTCTGAAAAAATTTGAAATTGCAAAGATGGCTTTTGCTTGGATGACACTATAAAGTTCCAAAATTATCTATCATTCATTAGATAAATCCTTTTATAGAAACAAAATAGATTTCTTTGCTAGAATTCTTTTAGAAAACGAACGTCCGTGAGGACTTCAAATCTTCGGTTAGATTGAATTGGCTGTTTTATTCAATTATTCATTTGTCTTAGGGAGGGATTGAAGATGGTGAAGACCCAAAGTTGGCAGCTGTTAGGGAACTGAGAAAGGAAACTGGAATAGTGTCTGCTGAAATCGTTGCCGAGGTATTACTTTGTTTGTTGATTTGTTTCAACGTCGTTTGTGATGAATTATTAAACCGACCCTTTCTACAGCATTTTCAAAGTCACCTCAACAATAGTACTGAAATAAATTCAAGAATTTGCATTGTTTTCGGTTAACAGGTACCCAAATGGTTGACTTACGACTTTCCTCCTGCTGTGAAGACCAAAGTCAATCGTCTCTGGGGCGGGCAATGGCACGGGCAGGCACAAAAATGGTCCGTCTTTCTGTCTTTTTTCATTCTAGTTGAAATTGAAATTAGACAAGATTGATGCAAAGAAATGAGGTTTATATGCAACTGTTTTGTCCTTGGTCTCATTTTGGAACTTATGACAGGTTTCTTATGAGATTGACAAAAGACGACAGTGAGATTAACTTGGACAACGATGAAGCGGATGTTGAATTTGCAGAGTGGAAATGGGCGAACCCCGAAGAAGTTGTTGAACAGGTCTTTTAAGACACTCGTTTGATAACGATTTCAAAAAGTTTTAAGGATCAAAATCAAGATTTTAGAATATACAAAGCTAAACTTACATACAAATATAGTAACATGTAATTAAAGCTTGTTTTTTTGTTCTTCTTTTTCAGGCTGTGGACTACAAGAGACCAACTTATGAGGAAGTAATGAAGACTTTTGCACCTTACCTTGATGGAAACAAAATATCTACCAAATGCAAATCATCCAAATGGTGAATGGTAAAAAATAATGGTGTTTTTACCTCAATTTTTATGAAGTAAAAAAGAAAGAAAGAAAAAAGAAAGATGAATTTGTAAATGAGGGAACTGCTAGAAAACTGCTTACTTATCTGATTTTGTTGGAATAATTGAAATTTTTGTCAAATTACTTTTAACAGTTTATGGGGGGAAATAACTGTGTGCTATTTTAGGAGATTTGCAGATCTTTGTAAAGAAAGGGGAAGTAGTGAAATGAAAGTGTATGAAAAGGTGTGTGTTGACTTTTTTTTTTTGTGATAATTAATTAATGTATGAATGTTTGGAATTTTTTTGTCTTTTTCTCCCTTTTGGATTTTGGTTGGAAG

mRNA sequence

ATGCAGGTTTTTGTGGCTTCTAGACTGAATGTTCCAGGAGCATGGCAGATGCCTCAGGTTAGTTGTGAACTTGTGAACTGTAATGGATTATCTGCCCTTTTGATATCGACGGCGTTTGAATCGGGAGGGATTGAAGATGGTGAAGACCCAAAGTTGGCAGCTGTTAGGGAACTGAGAAAGGAAACTGGAATAGTGTCTGCTGAAATCGTTGCCGAGGTACCCAAATGGTTGACTTACGACTTTCCTCCTGCTGTGAAGACCAAAGTCAATCGTCTCTGGGGCGGGCAATGGCACGGGCAGGCACAAAAATGGTTTCTTATGAGATTGACAAAAGACGACAGTGAGATTAACTTGGACAACGATGAAGCGGATGTTGAATTTGCAGAGTGGAAATGGGCGAACCCCGAAGAAGTTGTTGAACAGGCTGTGGACTACAAGAGACCAACTTATGAGGAAGTAATGAAGACTTTTGCACCTTACCTTGATGGAAACAAAATATCTACCAAATGCAAATCATCCAAATGGTGAATGGTAAAAAATAATGGTGTTTTTACCTCAATTTTTATGAAGTAAAAAAGAAAGAAAGAAAAAAGAAAGATGAATTTGTAAATGAGGGAACTGCTAGAAAACTGCTTACTTATCTGATTTTGTTGGAATAATTGAAATTTTTGTCAAATTACTTTTAACAGTTTATGGGGGGAAATAACTGTGTGCTATTTTAGGAGATTTGCAGATCTTTGTAAAGAAAGGGGAAGTAGTGAAATGAAAGTGTATGAAAAGGTGTGTGTTGACTTTTTTTTTTTGTGATAATTAATTAATGTATGAATGTTTGGAATTTTTTTGTCTTTTTCTCCCTTTTGGATTTTGGTTGGAAG

Coding sequence (CDS)

ATGCAGGTTTTTGTGGCTTCTAGACTGAATGTTCCAGGAGCATGGCAGATGCCTCAGGTTAGTTGTGAACTTGTGAACTGTAATGGATTATCTGCCCTTTTGATATCGACGGCGTTTGAATCGGGAGGGATTGAAGATGGTGAAGACCCAAAGTTGGCAGCTGTTAGGGAACTGAGAAAGGAAACTGGAATAGTGTCTGCTGAAATCGTTGCCGAGGTACCCAAATGGTTGACTTACGACTTTCCTCCTGCTGTGAAGACCAAAGTCAATCGTCTCTGGGGCGGGCAATGGCACGGGCAGGCACAAAAATGGTTTCTTATGAGATTGACAAAAGACGACAGTGAGATTAACTTGGACAACGATGAAGCGGATGTTGAATTTGCAGAGTGGAAATGGGCGAACCCCGAAGAAGTTGTTGAACAGGCTGTGGACTACAAGAGACCAACTTATGAGGAAGTAATGAAGACTTTTGCACCTTACCTTGATGGAAACAAAATATCTACCAAATGCAAATCATCCAAATGGTGA

Protein sequence

MQVFVASRLNVPGAWQMPQVSCELVNCNGLSALLISTAFESGGIEDGEDPKLAAVRELRKETGIVSAEIVAEVPKWLTYDFPPAVKTKVNRLWGGQWHGQAQKWFLMRLTKDDSEINLDNDEADVEFAEWKWANPEEVVEQAVDYKRPTYEEVMKTFAPYLDGNKISTKCKSSKW
Homology
BLAST of Lsi04G011630 vs. ExPASy Swiss-Prot
Match: Q9C6Z2 (Nudix hydrolase 25 OS=Arabidopsis thaliana OX=3702 GN=NUDT25 PE=1 SV=1)

HSP 1 Score: 245.7 bits (626), Expect = 3.8e-64
Identity = 120/175 (68.57%), Postives = 136/175 (77.71%), Query Frame = 0

Query: 3   VFVASRLNVPGAWQMPQVSCELVNCNGLSALLISTAFESGGIEDGEDPKLAAVRELRKET 62
           VFVASRLNVPGAWQMPQ                      GGIEDGEDPK AA+REL++ET
Sbjct: 23  VFVASRLNVPGAWQMPQ----------------------GGIEDGEDPKSAAMRELQEET 82

Query: 63  GIVSAEIVAEVPKWLTYDFPPAVKTKVNRLWGGQWHGQAQKWFLMRL--TKDDSEINLDN 122
           G+VSAEIV+EVP WLTYDFPPAVK KVNRLWGG+WHGQAQKW+L+RL   +D+ EINL N
Sbjct: 83  GVVSAEIVSEVPNWLTYDFPPAVKAKVNRLWGGEWHGQAQKWYLVRLRNDEDEKEINLAN 142

Query: 123 DEADVEFAEWKWANPEEVVEQAVDYKRPTYEEVMKTFAPYLDGNKISTKCKSSKW 176
           +EAD EFAEWKWA PEEVVEQAVDYKRPTYEEV+KTF  +L+    + KCKS+KW
Sbjct: 143 NEADSEFAEWKWAKPEEVVEQAVDYKRPTYEEVIKTFGSFLNDTGRAAKCKSAKW 175

BLAST of Lsi04G011630 vs. ExPASy Swiss-Prot
Match: Q9CAF2 (Nudix hydrolase 26, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=NUDT26 PE=1 SV=1)

HSP 1 Score: 172.2 bits (435), Expect = 5.3e-42
Identity = 80/161 (49.69%), Postives = 107/161 (66.46%), Query Frame = 0

Query: 2   QVFVASRLNVPGAWQMPQVSCELVNCNGLSALLISTAFESGGIEDGEDPKLAAVRELRKE 61
           ++F ASRL++P AWQMPQ                      GGI++GEDP++A +REL++E
Sbjct: 77  KIFTASRLDIPSAWQMPQ----------------------GGIDEGEDPRVAVMRELKEE 136

Query: 62  TGIVSAEIVAEVPKWLTYDFPPAVKTKVNRLWGGQWHGQAQKWFLMRLTKDDSEINLDND 121
           TG+ SAEI+AE P W+TYDFPP V+ K+   WG  W GQAQKWFL++ T  D EINL  D
Sbjct: 137 TGVHSAEILAEAPHWITYDFPPDVREKLKVRWGSDWKGQAQKWFLLKFTGKDEEINLLGD 196

Query: 122 EAD-VEFAEWKWANPEEVVEQAVDYKRPTYEEVMKTFAPYL 162
             +  EF EW W +P++VVE AV++K+P Y+EVM  FA +L
Sbjct: 197 GTEKPEFGEWSWTSPDQVVENAVEFKKPVYKEVMSAFASHL 215

BLAST of Lsi04G011630 vs. ExPASy Swiss-Prot
Match: Q9FNH4 (Nudix hydrolase 27, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=NUDT27 PE=1 SV=1)

HSP 1 Score: 155.6 bits (392), Expect = 5.1e-37
Identity = 77/160 (48.12%), Postives = 98/160 (61.25%), Query Frame = 0

Query: 2   QVFVASRLNVPGAWQMPQVSCELVNCNGLSALLISTAFESGGIEDGEDPKLAAVRELRKE 61
           ++F AS++++P  WQMPQ                      GG ++GED + AA RELR+E
Sbjct: 76  KIFTASKIHIPDTWQMPQ----------------------GGADEGEDLRNAAFRELREE 135

Query: 62  TGIVSAEIVAEVPKWLTYDFPPAVKTKVNRLWGGQWHGQAQKWFLMRLTKDDSEINLDND 121
           TG+ SAE +AE+P WLTYDFP  VK K+NR W   + GQAQKWFL + T  + EINL  D
Sbjct: 136 TGVTSAEFIAEIPNWLTYDFPREVKDKLNRKWRTSYKGQAQKWFLFKFTGKEEEINLLGD 195

Query: 122 -EADVEFAEWKWANPEEVVEQAVDYKRPTYEEVMKTFAPY 161
             A  EF  W W  PE+V+E AV +KRP YE V+K F PY
Sbjct: 196 GTAKPEFKVWSWMLPEQVIEHAVYFKRPVYEHVIKQFNPY 213

BLAST of Lsi04G011630 vs. ExPASy Swiss-Prot
Match: A3PMX6 (RNA pyrophosphohydrolase OS=Rhodobacter sphaeroides (strain ATCC 17029 / ATH 2.4.9) OX=349101 GN=rppH PE=3 SV=1)

HSP 1 Score: 124.8 bits (312), Expect = 9.7e-28
Identity = 68/162 (41.98%), Postives = 92/162 (56.79%), Query Frame = 0

Query: 3   VFVASRLNVP-GAWQMPQVSCELVNCNGLSALLISTAFESGGIEDGEDPKLAAVRELRKE 62
           +F   R++ P  AWQMPQ                      GGI++GE P+ AA+REL +E
Sbjct: 27  IFAGQRIDSPVPAWQMPQ----------------------GGIDEGEKPREAALRELWEE 86

Query: 63  TGIVS--AEIVAEVPKWLTYDFPPAVKTKVNRLWGGQWHGQAQKWFLMRLTKDDSEINLD 122
           TGI +   E VA+ P W+TYD PP +   + R+WGG++ GQ QKWFL R    D E+ + 
Sbjct: 87  TGIPAERVEFVAKAPDWVTYDLPPEL---LGRVWGGKYRGQRQKWFLYRYLGTDDEVGIG 146

Query: 123 NDEADVEFAEWKWANPEEVVEQAVDYKRPTYEEVMKTFAPYL 162
            D A  EF+ W+W   EE+V   V +KR  YEEV+ TF P+L
Sbjct: 147 TDHA--EFSCWRWIGAEEMVAAIVPFKRAVYEEVVATFRPHL 161

BLAST of Lsi04G011630 vs. ExPASy Swiss-Prot
Match: Q3IZC1 (RNA pyrophosphohydrolase OS=Rhodobacter sphaeroides (strain ATCC 17023 / DSM 158 / JCM 6121 / NBRC 12203 / NCIMB 8253 / ATH 2.4.1.) OX=272943 GN=rppH PE=3 SV=1)

HSP 1 Score: 124.8 bits (312), Expect = 9.7e-28
Identity = 68/162 (41.98%), Postives = 92/162 (56.79%), Query Frame = 0

Query: 3   VFVASRLNVP-GAWQMPQVSCELVNCNGLSALLISTAFESGGIEDGEDPKLAAVRELRKE 62
           +F   R++ P  AWQMPQ                      GGI++GE P+ AA+REL +E
Sbjct: 27  IFAGQRIDSPVPAWQMPQ----------------------GGIDEGEKPREAALRELWEE 86

Query: 63  TGIVS--AEIVAEVPKWLTYDFPPAVKTKVNRLWGGQWHGQAQKWFLMRLTKDDSEINLD 122
           TGI +   E VA+ P W+TYD PP +   + R+WGG++ GQ QKWFL R    D E+ + 
Sbjct: 87  TGIPAERVEFVAKAPDWVTYDLPPEL---LGRVWGGKYRGQRQKWFLYRYLGTDEEVGIG 146

Query: 123 NDEADVEFAEWKWANPEEVVEQAVDYKRPTYEEVMKTFAPYL 162
            D A  EF+ W+W   EE+V   V +KR  YEEV+ TF P+L
Sbjct: 147 TDHA--EFSCWRWIGAEEMVAAIVPFKRAVYEEVVATFRPHL 161

BLAST of Lsi04G011630 vs. ExPASy TrEMBL
Match: A0A0A0KL56 (Nudix hydrolase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G002050 PE=3 SV=1)

HSP 1 Score: 306.2 bits (783), Expect = 8.7e-80
Identity = 150/174 (86.21%), Postives = 152/174 (87.36%), Query Frame = 0

Query: 2   QVFVASRLNVPGAWQMPQVSCELVNCNGLSALLISTAFESGGIEDGEDPKLAAVRELRKE 61
           QVFVASRLNVPGAWQMPQ                      GGIEDGEDPKLAAVRELRKE
Sbjct: 22  QVFVASRLNVPGAWQMPQ----------------------GGIEDGEDPKLAAVRELRKE 81

Query: 62  TGIVSAEIVAEVPKWLTYDFPPAVKTKVNRLWGGQWHGQAQKWFLMRLTKDDSEINLDND 121
           TGIVSAE+VAEVPKWLTYDFPPAVKTKVNRLWGGQWHGQAQKWFLMRLTKDDSEINLDND
Sbjct: 82  TGIVSAEMVAEVPKWLTYDFPPAVKTKVNRLWGGQWHGQAQKWFLMRLTKDDSEINLDND 141

Query: 122 EADVEFAEWKWANPEEVVEQAVDYKRPTYEEVMKTFAPYLDGNKISTKCKSSKW 176
           EADVEFAEWKWANPEEVVEQAVDYKRPTYEEVMKTFAPYL+GNKISTKCKSSKW
Sbjct: 142 EADVEFAEWKWANPEEVVEQAVDYKRPTYEEVMKTFAPYLNGNKISTKCKSSKW 173

BLAST of Lsi04G011630 vs. ExPASy TrEMBL
Match: A0A6J1D8B1 (nudix hydrolase 25 OS=Momordica charantia OX=3673 GN=LOC111018292 PE=3 SV=1)

HSP 1 Score: 305.4 bits (781), Expect = 1.5e-79
Identity = 148/174 (85.06%), Postives = 151/174 (86.78%), Query Frame = 0

Query: 2   QVFVASRLNVPGAWQMPQVSCELVNCNGLSALLISTAFESGGIEDGEDPKLAAVRELRKE 61
           QVFVASRLNVPGAWQMPQ                      GGIEDGEDPKLAAVRELRKE
Sbjct: 22  QVFVASRLNVPGAWQMPQ----------------------GGIEDGEDPKLAAVRELRKE 81

Query: 62  TGIVSAEIVAEVPKWLTYDFPPAVKTKVNRLWGGQWHGQAQKWFLMRLTKDDSEINLDND 121
           TGIVSAEIVAEVP+WLTYDFPPAVKTKVNRLWGGQWHGQAQKWFLMR TKDDSEINLDND
Sbjct: 82  TGIVSAEIVAEVPQWLTYDFPPAVKTKVNRLWGGQWHGQAQKWFLMRFTKDDSEINLDND 141

Query: 122 EADVEFAEWKWANPEEVVEQAVDYKRPTYEEVMKTFAPYLDGNKISTKCKSSKW 176
           EAD+EFAEWKWANPEEV+EQAVDYKRPTYEEVMKTFAPYLDGNKISTKCKSSKW
Sbjct: 142 EADIEFAEWKWANPEEVIEQAVDYKRPTYEEVMKTFAPYLDGNKISTKCKSSKW 173

BLAST of Lsi04G011630 vs. ExPASy TrEMBL
Match: A0A1S3BZA8 (nudix hydrolase 25 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103495074 PE=3 SV=1)

HSP 1 Score: 304.7 bits (779), Expect = 2.5e-79
Identity = 148/174 (85.06%), Postives = 152/174 (87.36%), Query Frame = 0

Query: 2   QVFVASRLNVPGAWQMPQVSCELVNCNGLSALLISTAFESGGIEDGEDPKLAAVRELRKE 61
           QVFVAS+LNVPGAWQMPQ                      GGIEDGEDPKLAA+RELRKE
Sbjct: 22  QVFVASKLNVPGAWQMPQ----------------------GGIEDGEDPKLAAIRELRKE 81

Query: 62  TGIVSAEIVAEVPKWLTYDFPPAVKTKVNRLWGGQWHGQAQKWFLMRLTKDDSEINLDND 121
           TGIVSAE+VAEVPKWLTYDFPPAVKTKVNRLWGGQWHGQAQKWFLMRLTKDDSEINLDND
Sbjct: 82  TGIVSAEMVAEVPKWLTYDFPPAVKTKVNRLWGGQWHGQAQKWFLMRLTKDDSEINLDND 141

Query: 122 EADVEFAEWKWANPEEVVEQAVDYKRPTYEEVMKTFAPYLDGNKISTKCKSSKW 176
           EADVEFAEWKWANPEEVVEQAVDYKRPTYEEVMKTFAPYL+GNKISTKCKSSKW
Sbjct: 142 EADVEFAEWKWANPEEVVEQAVDYKRPTYEEVMKTFAPYLNGNKISTKCKSSKW 173

BLAST of Lsi04G011630 vs. ExPASy TrEMBL
Match: A0A6J1JPJ6 (nudix hydrolase 25 OS=Cucurbita maxima OX=3661 GN=LOC111488683 PE=3 SV=1)

HSP 1 Score: 294.7 bits (753), Expect = 2.6e-76
Identity = 144/174 (82.76%), Postives = 149/174 (85.63%), Query Frame = 0

Query: 2   QVFVASRLNVPGAWQMPQVSCELVNCNGLSALLISTAFESGGIEDGEDPKLAAVRELRKE 61
           QVFVASRLNVPGAWQMPQ                      GGIEDGED KLAAVREL+KE
Sbjct: 22  QVFVASRLNVPGAWQMPQ----------------------GGIEDGEDLKLAAVRELKKE 81

Query: 62  TGIVSAEIVAEVPKWLTYDFPPAVKTKVNRLWGGQWHGQAQKWFLMRLTKDDSEINLDND 121
           TG+VSAEIVAEVPKWLTYDFP AVKTKVNRLWGGQWHGQAQKWFLMR+TKDDSEINLDND
Sbjct: 82  TGVVSAEIVAEVPKWLTYDFPSAVKTKVNRLWGGQWHGQAQKWFLMRMTKDDSEINLDND 141

Query: 122 EADVEFAEWKWANPEEVVEQAVDYKRPTYEEVMKTFAPYLDGNKISTKCKSSKW 176
           EADVEFAEWKWA+PEEV+EQAVDYKRPTYEEVMKTFA YLDGNKISTKCKSSKW
Sbjct: 142 EADVEFAEWKWASPEEVIEQAVDYKRPTYEEVMKTFAAYLDGNKISTKCKSSKW 173

BLAST of Lsi04G011630 vs. ExPASy TrEMBL
Match: A0A6J1FLV4 (nudix hydrolase 25 OS=Cucurbita moschata OX=3662 GN=LOC111446990 PE=3 SV=1)

HSP 1 Score: 294.7 bits (753), Expect = 2.6e-76
Identity = 144/174 (82.76%), Postives = 149/174 (85.63%), Query Frame = 0

Query: 2   QVFVASRLNVPGAWQMPQVSCELVNCNGLSALLISTAFESGGIEDGEDPKLAAVRELRKE 61
           QVFVASRLNVPGAWQMPQ                      GGIEDGED KLAAVREL+KE
Sbjct: 22  QVFVASRLNVPGAWQMPQ----------------------GGIEDGEDLKLAAVRELKKE 81

Query: 62  TGIVSAEIVAEVPKWLTYDFPPAVKTKVNRLWGGQWHGQAQKWFLMRLTKDDSEINLDND 121
           TG+VSAEIVAEVPKWLTYDFP AVKTKVNRLWGGQWHGQAQKWFLMR+TKDDSEINLDND
Sbjct: 82  TGVVSAEIVAEVPKWLTYDFPSAVKTKVNRLWGGQWHGQAQKWFLMRMTKDDSEINLDND 141

Query: 122 EADVEFAEWKWANPEEVVEQAVDYKRPTYEEVMKTFAPYLDGNKISTKCKSSKW 176
           EADVEFAEWKWA+PEEV+EQAVDYKRPTYEEVMKTFA YLDGNKISTKCKSSKW
Sbjct: 142 EADVEFAEWKWASPEEVIEQAVDYKRPTYEEVMKTFAAYLDGNKISTKCKSSKW 173

BLAST of Lsi04G011630 vs. NCBI nr
Match: XP_004151519.1 (nudix hydrolase 25 [Cucumis sativus] >KGN49559.1 hypothetical protein Csa_018423 [Cucumis sativus])

HSP 1 Score: 306.2 bits (783), Expect = 1.8e-79
Identity = 150/174 (86.21%), Postives = 152/174 (87.36%), Query Frame = 0

Query: 2   QVFVASRLNVPGAWQMPQVSCELVNCNGLSALLISTAFESGGIEDGEDPKLAAVRELRKE 61
           QVFVASRLNVPGAWQMPQ                      GGIEDGEDPKLAAVRELRKE
Sbjct: 22  QVFVASRLNVPGAWQMPQ----------------------GGIEDGEDPKLAAVRELRKE 81

Query: 62  TGIVSAEIVAEVPKWLTYDFPPAVKTKVNRLWGGQWHGQAQKWFLMRLTKDDSEINLDND 121
           TGIVSAE+VAEVPKWLTYDFPPAVKTKVNRLWGGQWHGQAQKWFLMRLTKDDSEINLDND
Sbjct: 82  TGIVSAEMVAEVPKWLTYDFPPAVKTKVNRLWGGQWHGQAQKWFLMRLTKDDSEINLDND 141

Query: 122 EADVEFAEWKWANPEEVVEQAVDYKRPTYEEVMKTFAPYLDGNKISTKCKSSKW 176
           EADVEFAEWKWANPEEVVEQAVDYKRPTYEEVMKTFAPYL+GNKISTKCKSSKW
Sbjct: 142 EADVEFAEWKWANPEEVVEQAVDYKRPTYEEVMKTFAPYLNGNKISTKCKSSKW 173

BLAST of Lsi04G011630 vs. NCBI nr
Match: XP_022150013.1 (nudix hydrolase 25 [Momordica charantia])

HSP 1 Score: 305.4 bits (781), Expect = 3.1e-79
Identity = 148/174 (85.06%), Postives = 151/174 (86.78%), Query Frame = 0

Query: 2   QVFVASRLNVPGAWQMPQVSCELVNCNGLSALLISTAFESGGIEDGEDPKLAAVRELRKE 61
           QVFVASRLNVPGAWQMPQ                      GGIEDGEDPKLAAVRELRKE
Sbjct: 22  QVFVASRLNVPGAWQMPQ----------------------GGIEDGEDPKLAAVRELRKE 81

Query: 62  TGIVSAEIVAEVPKWLTYDFPPAVKTKVNRLWGGQWHGQAQKWFLMRLTKDDSEINLDND 121
           TGIVSAEIVAEVP+WLTYDFPPAVKTKVNRLWGGQWHGQAQKWFLMR TKDDSEINLDND
Sbjct: 82  TGIVSAEIVAEVPQWLTYDFPPAVKTKVNRLWGGQWHGQAQKWFLMRFTKDDSEINLDND 141

Query: 122 EADVEFAEWKWANPEEVVEQAVDYKRPTYEEVMKTFAPYLDGNKISTKCKSSKW 176
           EAD+EFAEWKWANPEEV+EQAVDYKRPTYEEVMKTFAPYLDGNKISTKCKSSKW
Sbjct: 142 EADIEFAEWKWANPEEVIEQAVDYKRPTYEEVMKTFAPYLDGNKISTKCKSSKW 173

BLAST of Lsi04G011630 vs. NCBI nr
Match: XP_008454739.1 (PREDICTED: nudix hydrolase 25 isoform X1 [Cucumis melo])

HSP 1 Score: 304.7 bits (779), Expect = 5.2e-79
Identity = 148/174 (85.06%), Postives = 152/174 (87.36%), Query Frame = 0

Query: 2   QVFVASRLNVPGAWQMPQVSCELVNCNGLSALLISTAFESGGIEDGEDPKLAAVRELRKE 61
           QVFVAS+LNVPGAWQMPQ                      GGIEDGEDPKLAA+RELRKE
Sbjct: 22  QVFVASKLNVPGAWQMPQ----------------------GGIEDGEDPKLAAIRELRKE 81

Query: 62  TGIVSAEIVAEVPKWLTYDFPPAVKTKVNRLWGGQWHGQAQKWFLMRLTKDDSEINLDND 121
           TGIVSAE+VAEVPKWLTYDFPPAVKTKVNRLWGGQWHGQAQKWFLMRLTKDDSEINLDND
Sbjct: 82  TGIVSAEMVAEVPKWLTYDFPPAVKTKVNRLWGGQWHGQAQKWFLMRLTKDDSEINLDND 141

Query: 122 EADVEFAEWKWANPEEVVEQAVDYKRPTYEEVMKTFAPYLDGNKISTKCKSSKW 176
           EADVEFAEWKWANPEEVVEQAVDYKRPTYEEVMKTFAPYL+GNKISTKCKSSKW
Sbjct: 142 EADVEFAEWKWANPEEVVEQAVDYKRPTYEEVMKTFAPYLNGNKISTKCKSSKW 173

BLAST of Lsi04G011630 vs. NCBI nr
Match: XP_038891479.1 (nudix hydrolase 25 [Benincasa hispida])

HSP 1 Score: 300.4 bits (768), Expect = 9.8e-78
Identity = 148/174 (85.06%), Postives = 149/174 (85.63%), Query Frame = 0

Query: 2   QVFVASRLNVPGAWQMPQVSCELVNCNGLSALLISTAFESGGIEDGEDPKLAAVRELRKE 61
           QVFVASRLNVPGAWQMPQ                      G IEDGEDPKLAAVRELRKE
Sbjct: 22  QVFVASRLNVPGAWQMPQ----------------------GEIEDGEDPKLAAVRELRKE 81

Query: 62  TGIVSAEIVAEVPKWLTYDFPPAVKTKVNRLWGGQWHGQAQKWFLMRLTKDDSEINLDND 121
           TGIVSAEIVAEVPKWLTYDFPPAVKTKV+RLWGGQWHGQAQKWFLMRLTKDDSEINLDND
Sbjct: 82  TGIVSAEIVAEVPKWLTYDFPPAVKTKVSRLWGGQWHGQAQKWFLMRLTKDDSEINLDND 141

Query: 122 EADVEFAEWKWANPEEVVEQAVDYKRPTYEEVMKTFAPYLDGNKISTKCKSSKW 176
           EADVEFAEWKWANP EVVEQAVDYKRPTYEEVMKTF PYLDGNKISTKCKSSKW
Sbjct: 142 EADVEFAEWKWANPAEVVEQAVDYKRPTYEEVMKTFGPYLDGNKISTKCKSSKW 173

BLAST of Lsi04G011630 vs. NCBI nr
Match: XP_022941711.1 (nudix hydrolase 25 [Cucurbita moschata])

HSP 1 Score: 294.7 bits (753), Expect = 5.4e-76
Identity = 144/174 (82.76%), Postives = 149/174 (85.63%), Query Frame = 0

Query: 2   QVFVASRLNVPGAWQMPQVSCELVNCNGLSALLISTAFESGGIEDGEDPKLAAVRELRKE 61
           QVFVASRLNVPGAWQMPQ                      GGIEDGED KLAAVREL+KE
Sbjct: 22  QVFVASRLNVPGAWQMPQ----------------------GGIEDGEDLKLAAVRELKKE 81

Query: 62  TGIVSAEIVAEVPKWLTYDFPPAVKTKVNRLWGGQWHGQAQKWFLMRLTKDDSEINLDND 121
           TG+VSAEIVAEVPKWLTYDFP AVKTKVNRLWGGQWHGQAQKWFLMR+TKDDSEINLDND
Sbjct: 82  TGVVSAEIVAEVPKWLTYDFPSAVKTKVNRLWGGQWHGQAQKWFLMRMTKDDSEINLDND 141

Query: 122 EADVEFAEWKWANPEEVVEQAVDYKRPTYEEVMKTFAPYLDGNKISTKCKSSKW 176
           EADVEFAEWKWA+PEEV+EQAVDYKRPTYEEVMKTFA YLDGNKISTKCKSSKW
Sbjct: 142 EADVEFAEWKWASPEEVIEQAVDYKRPTYEEVMKTFAAYLDGNKISTKCKSSKW 173

BLAST of Lsi04G011630 vs. TAIR 10
Match: AT1G30110.1 (nudix hydrolase homolog 25 )

HSP 1 Score: 245.7 bits (626), Expect = 2.7e-65
Identity = 120/175 (68.57%), Postives = 136/175 (77.71%), Query Frame = 0

Query: 3   VFVASRLNVPGAWQMPQVSCELVNCNGLSALLISTAFESGGIEDGEDPKLAAVRELRKET 62
           VFVASRLNVPGAWQMPQ                      GGIEDGEDPK AA+REL++ET
Sbjct: 23  VFVASRLNVPGAWQMPQ----------------------GGIEDGEDPKSAAMRELQEET 82

Query: 63  GIVSAEIVAEVPKWLTYDFPPAVKTKVNRLWGGQWHGQAQKWFLMRL--TKDDSEINLDN 122
           G+VSAEIV+EVP WLTYDFPPAVK KVNRLWGG+WHGQAQKW+L+RL   +D+ EINL N
Sbjct: 83  GVVSAEIVSEVPNWLTYDFPPAVKAKVNRLWGGEWHGQAQKWYLVRLRNDEDEKEINLAN 142

Query: 123 DEADVEFAEWKWANPEEVVEQAVDYKRPTYEEVMKTFAPYLDGNKISTKCKSSKW 176
           +EAD EFAEWKWA PEEVVEQAVDYKRPTYEEV+KTF  +L+    + KCKS+KW
Sbjct: 143 NEADSEFAEWKWAKPEEVVEQAVDYKRPTYEEVIKTFGSFLNDTGRAAKCKSAKW 175

BLAST of Lsi04G011630 vs. TAIR 10
Match: AT1G30110.2 (nudix hydrolase homolog 25 )

HSP 1 Score: 245.7 bits (626), Expect = 2.7e-65
Identity = 120/175 (68.57%), Postives = 136/175 (77.71%), Query Frame = 0

Query: 3   VFVASRLNVPGAWQMPQVSCELVNCNGLSALLISTAFESGGIEDGEDPKLAAVRELRKET 62
           VFVASRLNVPGAWQMPQ                      GGIEDGEDPK AA+REL++ET
Sbjct: 23  VFVASRLNVPGAWQMPQ----------------------GGIEDGEDPKSAAMRELQEET 82

Query: 63  GIVSAEIVAEVPKWLTYDFPPAVKTKVNRLWGGQWHGQAQKWFLMRL--TKDDSEINLDN 122
           G+VSAEIV+EVP WLTYDFPPAVK KVNRLWGG+WHGQAQKW+L+RL   +D+ EINL N
Sbjct: 83  GVVSAEIVSEVPNWLTYDFPPAVKAKVNRLWGGEWHGQAQKWYLVRLRNDEDEKEINLAN 142

Query: 123 DEADVEFAEWKWANPEEVVEQAVDYKRPTYEEVMKTFAPYLDGNKISTKCKSSKW 176
           +EAD EFAEWKWA PEEVVEQAVDYKRPTYEEV+KTF  +L+    + KCKS+KW
Sbjct: 143 NEADSEFAEWKWAKPEEVVEQAVDYKRPTYEEVIKTFGSFLNDTGRAAKCKSAKW 175

BLAST of Lsi04G011630 vs. TAIR 10
Match: AT3G10620.1 (nudix hydrolase homolog 26 )

HSP 1 Score: 172.2 bits (435), Expect = 3.8e-43
Identity = 80/161 (49.69%), Postives = 107/161 (66.46%), Query Frame = 0

Query: 2   QVFVASRLNVPGAWQMPQVSCELVNCNGLSALLISTAFESGGIEDGEDPKLAAVRELRKE 61
           ++F ASRL++P AWQMPQ                      GGI++GEDP++A +REL++E
Sbjct: 77  KIFTASRLDIPSAWQMPQ----------------------GGIDEGEDPRVAVMRELKEE 136

Query: 62  TGIVSAEIVAEVPKWLTYDFPPAVKTKVNRLWGGQWHGQAQKWFLMRLTKDDSEINLDND 121
           TG+ SAEI+AE P W+TYDFPP V+ K+   WG  W GQAQKWFL++ T  D EINL  D
Sbjct: 137 TGVHSAEILAEAPHWITYDFPPDVREKLKVRWGSDWKGQAQKWFLLKFTGKDEEINLLGD 196

Query: 122 EAD-VEFAEWKWANPEEVVEQAVDYKRPTYEEVMKTFAPYL 162
             +  EF EW W +P++VVE AV++K+P Y+EVM  FA +L
Sbjct: 197 GTEKPEFGEWSWTSPDQVVENAVEFKKPVYKEVMSAFASHL 215

BLAST of Lsi04G011630 vs. TAIR 10
Match: AT5G06340.1 (nudix hydrolase homolog 27 )

HSP 1 Score: 155.6 bits (392), Expect = 3.6e-38
Identity = 77/160 (48.12%), Postives = 98/160 (61.25%), Query Frame = 0

Query: 2   QVFVASRLNVPGAWQMPQVSCELVNCNGLSALLISTAFESGGIEDGEDPKLAAVRELRKE 61
           ++F AS++++P  WQMPQ                      GG ++GED + AA RELR+E
Sbjct: 76  KIFTASKIHIPDTWQMPQ----------------------GGADEGEDLRNAAFRELREE 135

Query: 62  TGIVSAEIVAEVPKWLTYDFPPAVKTKVNRLWGGQWHGQAQKWFLMRLTKDDSEINLDND 121
           TG+ SAE +AE+P WLTYDFP  VK K+NR W   + GQAQKWFL + T  + EINL  D
Sbjct: 136 TGVTSAEFIAEIPNWLTYDFPREVKDKLNRKWRTSYKGQAQKWFLFKFTGKEEEINLLGD 195

Query: 122 -EADVEFAEWKWANPEEVVEQAVDYKRPTYEEVMKTFAPY 161
             A  EF  W W  PE+V+E AV +KRP YE V+K F PY
Sbjct: 196 GTAKPEFKVWSWMLPEQVIEHAVYFKRPVYEHVIKQFNPY 213

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9C6Z23.8e-6468.57Nudix hydrolase 25 OS=Arabidopsis thaliana OX=3702 GN=NUDT25 PE=1 SV=1[more]
Q9CAF25.3e-4249.69Nudix hydrolase 26, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=NUDT26 PE=1... [more]
Q9FNH45.1e-3748.13Nudix hydrolase 27, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=NUDT27 PE=1... [more]
A3PMX69.7e-2841.98RNA pyrophosphohydrolase OS=Rhodobacter sphaeroides (strain ATCC 17029 / ATH 2.4... [more]
Q3IZC19.7e-2841.98RNA pyrophosphohydrolase OS=Rhodobacter sphaeroides (strain ATCC 17023 / DSM 158... [more]
Match NameE-valueIdentityDescription
A0A0A0KL568.7e-8086.21Nudix hydrolase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G00... [more]
A0A6J1D8B11.5e-7985.06nudix hydrolase 25 OS=Momordica charantia OX=3673 GN=LOC111018292 PE=3 SV=1[more]
A0A1S3BZA82.5e-7985.06nudix hydrolase 25 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103495074 PE=3 SV=1[more]
A0A6J1JPJ62.6e-7682.76nudix hydrolase 25 OS=Cucurbita maxima OX=3661 GN=LOC111488683 PE=3 SV=1[more]
A0A6J1FLV42.6e-7682.76nudix hydrolase 25 OS=Cucurbita moschata OX=3662 GN=LOC111446990 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_004151519.11.8e-7986.21nudix hydrolase 25 [Cucumis sativus] >KGN49559.1 hypothetical protein Csa_018423... [more]
XP_022150013.13.1e-7985.06nudix hydrolase 25 [Momordica charantia][more]
XP_008454739.15.2e-7985.06PREDICTED: nudix hydrolase 25 isoform X1 [Cucumis melo][more]
XP_038891479.19.8e-7885.06nudix hydrolase 25 [Benincasa hispida][more]
XP_022941711.15.4e-7682.76nudix hydrolase 25 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
AT1G30110.12.7e-6568.57nudix hydrolase homolog 25 [more]
AT1G30110.22.7e-6568.57nudix hydrolase homolog 25 [more]
AT3G10620.13.8e-4349.69nudix hydrolase homolog 26 [more]
AT5G06340.13.6e-3848.13nudix hydrolase homolog 27 [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.90.79.10Nucleoside Triphosphate Pyrophosphohydrolasecoord: 1..161
e-value: 1.6E-37
score: 130.8
NoneNo IPR availablePANTHERPTHR11839UDP/ADP-SUGAR PYROPHOSPHATASEcoord: 40..175
coord: 3..23
NoneNo IPR availablePANTHERPTHR11839:SF27BNACNNG74470D PROTEINcoord: 40..175
coord: 3..23
IPR000086NUDIX hydrolase domainPFAMPF00293NUDIXcoord: 12..151
e-value: 8.0E-15
score: 55.0
IPR000086NUDIX hydrolase domainPROSITEPS51462NUDIXcoord: 1..155
score: 16.12043
IPR022927RNA pyrophosphohydrolase RppHCDDcd03671Ap4A_hydrolase_plant_likecoord: 2..157
e-value: 1.23338E-57
score: 175.472
IPR015797NUDIX hydrolase-like domain superfamilySUPERFAMILY55811Nudixcoord: 41..160

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi04G011630.1Lsi04G011630.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0015967 diadenosine tetraphosphate catabolic process
biological_process GO:0019693 ribose phosphate metabolic process
cellular_component GO:0009507 chloroplast
molecular_function GO:0034432 bis(5'-adenosyl)-pentaphosphatase activity
molecular_function GO:0004081 bis(5'-nucleosyl)-tetraphosphatase (asymmetrical) activity
molecular_function GO:0008893 guanosine-3',5'-bis(diphosphate) 3'-diphosphatase activity