Homology
BLAST of Lsi04G009180 vs. ExPASy Swiss-Prot
Match:
Q39547 (Cucumisin OS=Cucumis melo OX=3656 PE=1 SV=1)
HSP 1 Score: 1003.8 bits (2594), Expect = 9.6e-292
Identity = 523/733 (71.35%), Postives = 584/733 (79.67%), Query Frame = 0
Query: 7 MSSSLIFKLLFLNIFFSIQIASSLDSDNDGKKIYIVYMGRKLKDDRDSAHLHQSSF---- 66
MSSSLIFKL F ++FFS ++AS LDSD+DGK IYIVYMGRKL +D DSAHLH +
Sbjct: 1 MSSSLIFKLFFFSLFFSNRLASRLDSDDDGKNIYIVYMGRKL-EDPDSAHLHHRAMLEQV 60
Query: 67 ---PFAPESVVYTYKRSFNGFAVKLTEEEAEKIAGMEGVVSVFPNKMKQLHTSRSWDFLG 126
FAPESV++TYKRSFNGFAVKLTEEEAEKIA MEGVVSVF N+M +LHT+RSWDFLG
Sbjct: 61 VGSTFAPESVLHTYKRSFNGFAVKLTEEEAEKIASMEGVVSVFLNEMNELHTTRSWDFLG 120
Query: 127 FPKNVPRVKQVESNIIVGVLDTGIWPESPSFDDKGFGPIPSKWKGTCQ-GLNFTC----- 186
FP VPR QVESNI+VGVLDTGIWPESPSFDD+GF P P KWKGTC+ NF C
Sbjct: 121 FPLTVPRRSQVESNIVVGVLDTGIWPESPSFDDEGFSPPPPKWKGTCETSNNFRCNRKII 180
Query: 187 ----------------NSPRDINGHGTHTASTVAGSLVSHASLYGVGLGTARGGVPSARI 246
N PRD NGHGTHTAST AG LVS A+LYG+GLGTARGGVP ARI
Sbjct: 181 GARSYHIGRPISPGDVNGPRDTNGHGTHTASTAAGGLVSQANLYGLGLGTARGGVPLARI 240
Query: 247 AAYKICWNKIGCSQANILAAFDDAIADGVDIISISVGDGDLQPYFLDSVGIGSFHAIQNG 306
AAYK+CWN GCS +ILAA+DDAIADGVDIIS+SVG + + YF+D++ IGSFHA++ G
Sbjct: 241 AAYKVCWND-GCSDTDILAAYDDAIADGVDIISLSVGGANPRHYFVDAIAIGSFHAVERG 300
Query: 307 ILTSNSVGNSGPKLYSTTSVSPWLLSVAASTIDRKIVTQVQIGNKKTFQGVSINIFDMNG 366
ILTSNS GN GP ++T S+SPWLLSVAAST+DRK VTQVQIGN ++FQGVSIN FD N
Sbjct: 301 ILTSNSAGNGGPNFFTTASLSPWLLSVAASTMDRKFVTQVQIGNGQSFQGVSINTFD-NQ 360
Query: 367 KYPLVAGHDVLKAGFHNSISRSCRNNSVNPKLVKGKILICKELLSRDDFF-SFGGAAGVL 426
YPLV+G D+ GF S SR C + SVNP L+KGKI++C+ +FF S GAAGVL
Sbjct: 361 YYPLVSGRDIPNTGFDKSTSRFCTDKSVNPNLLKGKIVVCEASFGPHEFFKSLDGAAGVL 420
Query: 427 MVSGTRDTAFPQPLPSSTLDPNDADTIFHYIDSARSPTATIFKSTTLHNVSAPSLASFSS 486
M S TRD A PLPSS LDPND YI S RSP ATIFKSTT+ N SAP + SFSS
Sbjct: 421 MTSNTRDYADSYPLPSSVLDPNDLLATLRYIYSIRSPGATIFKSTTILNASAPVVVSFSS 480
Query: 487 RGPNVLNKDLVKPDLSAPGVEILAAWPPVAPVDGSHR-TLYNIVSGTSMACPHITGIAVY 546
RGPN KD++KPD+S PGVEILAAWP VAPV G R TL+NI+SGTSM+CPHITGIA Y
Sbjct: 481 RGPNRATKDVIKPDISGPGVEILAAWPSVAPVGGIRRNTLFNIISGTSMSCPHITGIATY 540
Query: 547 VKTFNPTWSPAAIKSALMTTASPMNSRFHPEAEFAYGSGHVNPLKAVRPGLVYDANESDY 606
VKT+NPTWSPAAIKSALMTTASPMN+RF+P+AEFAYGSGHVNPLKAVRPGLVYDANESDY
Sbjct: 541 VKTYNPTWSPAAIKSALMTTASPMNARFNPQAEFAYGSGHVNPLKAVRPGLVYDANESDY 600
Query: 607 VKFLCGQGYTTNMVRTITNDNSACTSENIGRVWDLNSPSFGLSVSHSKTFNQYFTRTLTS 666
VKFLCGQGY T VR IT D SACTS N GRVWDLN PSFGLSVS S+TFNQYF RTLTS
Sbjct: 601 VKFLCGQGYNTQAVRRITGDYSACTSGNTGRVWDLNYPSFGLSVSPSQTFNQYFNRTLTS 660
Query: 667 VASQASTYRAMISTPKGLSITVNPNVLSFNGIGDRKSFKLTVRGTIRKDTVSAFLVWSDG 709
VA QASTYRAMIS P+GL+I+VNPNVLSFNG+GDRKSF LTVRG+I+ VSA LVWSDG
Sbjct: 661 VAPQASTYRAMISAPQGLTISVNPNVLSFNGLGDRKSFTLTVRGSIKGFVVSASLVWSDG 720
BLAST of Lsi04G009180 vs. ExPASy Swiss-Prot
Match:
Q9FIF8 (Subtilisin-like protease SBT4.3 OS=Arabidopsis thaliana OX=3702 GN=SBT4.3 PE=3 SV=1)
HSP 1 Score: 594.3 bits (1531), Expect = 1.7e-168
Identity = 328/711 (46.13%), Postives = 453/711 (63.71%), Query Frame = 0
Query: 29 SLDSDNDGKKIYIVYMGR--KLKDDRDSAHL----HQSSFPFAPESVVYTYKRSFNGFAV 88
S + +YIVYMG ++K S HL A +V +YKRSFNGFA
Sbjct: 22 SANDYRQASSVYIVYMGTLPEIKYSPPSHHLSILQKLVGTIAASHLLVRSYKRSFNGFAA 81
Query: 89 KLTEEEAEKIAGMEGVVSVFPNKMKQLHTSRSWDFLGFPKNVPRVKQVESNIIVGVLDTG 148
L++ E++K+ M+ VVSVFP+K +L T+RSWDF+GF + R ES++IVGV+D+G
Sbjct: 82 NLSQAESQKLQNMKEVVSVFPSKSHELTTTRSWDFVGFGEKARRESVKESDVIVGVIDSG 141
Query: 149 IWPESPSFDDKGFGPIPSKWKGTCQ-GLNFTCN--------------SPRDINGHGTHTA 208
IWPES SFDD+GFGP P KWKG+C+ GL F CN S RD GHGTHTA
Sbjct: 142 IWPESESFDDEGFGPPPKKWKGSCKGGLKFACNNKLIGARFYNKFADSARDEEGHGTHTA 201
Query: 209 STVAGSLVSHASLYGVGLGTARGGVPSARIAAYKICWNKIGCSQANILAAFDDAIADGVD 268
ST AG+ V AS YG+ GTARGGVPSARIAAYK+C+N+ C+ +ILAAFDDAIADGVD
Sbjct: 202 STAAGNAVQAASFYGLAQGTARGGVPSARIAAYKVCFNR--CNDVDILAAFDDAIADGVD 261
Query: 269 IISISVGDGDLQPYFLDSVGIGSFHAIQNGILTSNSVGNSGPKLYSTTSVSPWLLSVAAS 328
+ISIS+ + SV IGSFHA+ GI+T+ S GN+GP S +VSPW+++VAAS
Sbjct: 262 VISISISADYVSNLLNASVAIGSFHAMMRGIITAGSAGNNGPDQGSVANVSPWMITVAAS 321
Query: 329 TIDRKIVTQVQIGNKKTFQGVSINIFDMNG-KYPLVAGHDVLKAGFHNSISRSCRNNSVN 388
DR+ + +V +GN K G+S+N F++NG K+P+V G +V + + + C + V+
Sbjct: 322 GTDRQFIDRVVLGNGKALTGISVNTFNLNGTKFPIVYGQNVSR-NCSQAQAGYCSSGCVD 381
Query: 389 PKLVKGKILICKELLSRDDFFSFGGAAGVLMVSGTRDTAFPQPLPSSTLDPNDADTIFHY 448
+LVKGKI++C + L + + G ++ + D+AF P P+S+L D +I Y
Sbjct: 382 SELVKGKIVLCDDFLGYREAYLAGAIGVIVQNTLLPDSAFVVPFPASSLGFEDYKSIKSY 441
Query: 449 IDSARSPTATIFKSTTLHNVSAPSLASFSSRGPNVLNKDLVKPDLSAPGVEILAAWPPVA 508
I+SA P A I ++ + + AP + SFSSRGP+ + ++L+KPD+SAPG+EILAA+ PVA
Sbjct: 442 IESAEPPQAEILRTEEIVDREAPYVPSFSSRGPSFVIQNLLKPDVSAPGLEILAAFSPVA 501
Query: 509 P------VDGSHRTLYNIVSGTSMACPHITGIAVYVKTFNPTWSPAAIKSALMTTASPMN 568
+ Y+++SGTSMACPH+ G+A YVK+F+P WSP+AIKSA+MTTA+PMN
Sbjct: 502 SPSSFLNPEDKRSVRYSVMSGTSMACPHVAGVAAYVKSFHPDWSPSAIKSAIMTTATPMN 561
Query: 569 SRFHPEAEFAYGSGHVNPLKAVRPGLVYDANESDYVKFLCGQGYTTNMVRTITNDNSACT 628
+ +PE EFAYGSG +NP KA PGLVY+ DY+K LC +G+ + + T + N C+
Sbjct: 562 LKKNPEQEFAYGSGQINPTKASDPGLVYEVETEDYLKMLCAEGFDSTTLTTTSGQNVTCS 621
Query: 629 SENIGRVWDLNSPSFGLSVSHSKTFNQYFTRTLTSVASQASTYRA-MISTPKGLSITVNP 688
V DLN P+ VS FN F RT+T+V STY+A ++ L I++ P
Sbjct: 622 ERT--EVKDLNYPTMTTFVSSLDPFNVTFKRTVTNVGFPNSTYKASVVPLQPELQISIEP 681
Query: 689 NVLSFNGIGDRKSFKLTVRGTIRKD--TVSAFLVWSDGVHTVRSPITITSL 709
+L F + ++KSF +T+ G KD VS+ +VWSDG H+VRSPI S+
Sbjct: 682 EILRFGFLEEKKSFVVTISGKELKDGSFVSSSVVWSDGSHSVRSPIVAYSI 727
BLAST of Lsi04G009180 vs. ExPASy Swiss-Prot
Match:
Q9FIG2 (Subtilisin-like protease SBT4.13 OS=Arabidopsis thaliana OX=3702 GN=SBT4.13 PE=2 SV=1)
HSP 1 Score: 593.6 bits (1529), Expect = 3.0e-168
Identity = 338/731 (46.24%), Postives = 458/731 (62.65%), Query Frame = 0
Query: 8 SSSLIFKLLFLNIFFSIQIASSLDSDNDGKKIYIVYMGRKLKDDRD----SAHLHQSSFP 67
SSSL+ LL L SS+ + D K++YIVYMG L D S H++
Sbjct: 7 SSSLLSCLLVL-------FLSSVSAVTDDKQVYIVYMG-SLSSRADYTPTSDHMNILQEV 66
Query: 68 FAPESV----VYTYKRSFNGFAVKLTEEEAEKIAGMEGVVSVFPNKMKQLHTSRSWDFLG 127
S+ V +YKRSFNGFA +LTE E E++A M GVVSVFPNK QL T+ SWDF+G
Sbjct: 67 TGESSIEGRLVRSYKRSFNGFAARLTESERERVAKMVGVVSVFPNKKLQLQTTTSWDFMG 126
Query: 128 FPKNV--PRVKQVESNIIVGVLDTGIWPESPSFDDKGFGPIPSKWKGTCQ-GLNFTCNS- 187
+ + R VES+ I+GV+D+GI PES SF DKGFGP P KWKG C G NFTCN+
Sbjct: 127 LKEGIKTKRNPTVESDTIIGVIDSGITPESQSFSDKGFGPPPQKWKGVCSGGKNFTCNNK 186
Query: 188 -----------PRDINGHGTHTASTVAGSLVSHASLYGVGLGTARGGVPSARIAAYKICW 247
RD++GHGTHTAST AG+ V AS +G+G GT RGGVP++R+AAYK+C
Sbjct: 187 LIGARDYTSEGTRDMDGHGTHTASTAAGNAVVDASFFGIGNGTVRGGVPASRVAAYKVC- 246
Query: 248 NKIGCSQANILAAFDDAIADGVDIISISVGDGDLQPYFLDSVGIGSFHAIQNGILTSNSV 307
GCS +L+AFDDAIADGVD+I+IS+GD + D + IG+FHA+ G+LT NS
Sbjct: 247 TPTGCSSEALLSAFDDAIADGVDLITISIGDKTASMFQNDPIAIGAFHAMAKGVLTVNSA 306
Query: 308 GNSGPKLYSTTSVSPWLLSVAASTIDRKIVTQVQIGNKKTFQGVSINIFDMNGK-YPLVA 367
GNSGPK S + V+PW+L+VAAST +R VT+V +GN KT G S+N ++M GK YPLV
Sbjct: 307 GNSGPKPISVSGVAPWILTVAASTTNRGFVTKVVLGNGKTLVGKSVNAYEMKGKDYPLVY 366
Query: 368 GHDVLKAGFHNSISRSCRNNSVNPKLVKGKILICKELLSRDDFFSFGGAAGVLMVSGTRD 427
G + + C + V+ VKGKIL+C S GA G++ + D
Sbjct: 367 GKSAASSACDAESAGLCELSCVDKSRVKGKILVCGGPGGLKIVESV-GAVGLIYRTPKPD 426
Query: 428 TAFPQPLPSSTLDPNDADTIFHYIDSARSPTATIFKSTTLHNVSAPSLASFSSRGPNVLN 487
AF PLP++ L D +++ Y++S SP A + K+ + N ++P +ASFSSRGPN +
Sbjct: 427 VAFIHPLPAAGLLTEDFESLVSYLESTDSPQAIVLKTEAIFNRTSPVIASFSSRGPNTIA 486
Query: 488 KDLVKPDLSAPGVEILAAWPPVA--PVDGSHRTLYNIVSGTSMACPHITGIAVYVKTFNP 547
D++KPD++APGVEILAA+ P D + Y+++SGTSM+CPH+ G+A YVKTFNP
Sbjct: 487 VDILKPDITAPGVEILAAYSPAGEPSQDDTRHVKYSVLSGTSMSCPHVAGVAAYVKTFNP 546
Query: 548 TWSPAAIKSALMTTASPMNSRFH--PEAEFAYGSGHVNPLKAVRPGLVYDANESDYVKFL 607
WSP+ I+SA+MTTA P+N+ EFAYGSGHV+P+ A PGLVY+ ++SD++ FL
Sbjct: 547 KWSPSMIQSAIMTTAWPVNATGTGIASTEFAYGSGHVDPIAASNPGLVYELDKSDHIAFL 606
Query: 608 CGQGYTTNMVRTITNDNSACTSENIGRVWDLNSPSFGLSVSHS-KTFNQYFTRTLTSVAS 667
CG YT+ +++ I+ + C+ +LN PS +S S TF F RTLT+V +
Sbjct: 607 CGMNYTSQVLKVISGETVTCSEAKKILPRNLNYPSMSAKLSGSGTTFTVTFNRTLTNVGT 666
Query: 668 QASTYRAMISTPKG--LSITVNPNVLSFNGIGDRKSFKLTVRGTIRKDTV--SAFLVWSD 706
STY + + G L + + P+VLSF + +++SF +TV G+ V SA L+WSD
Sbjct: 667 PNSTYTSKVVAGHGSKLDVKITPSVLSFKTVNEKQSFTVTVTGSNLDSEVPSSANLIWSD 726
BLAST of Lsi04G009180 vs. ExPASy Swiss-Prot
Match:
Q9FGU3 (Subtilisin-like protease SBT4.4 OS=Arabidopsis thaliana OX=3702 GN=SBT4.4 PE=2 SV=1)
HSP 1 Score: 585.5 bits (1508), Expect = 8.1e-166
Identity = 331/746 (44.37%), Postives = 472/746 (63.27%), Query Frame = 0
Query: 7 MSSSLIFKLLFLNIFFSIQIASSLDSDNDG-KKIYIVYMGR-KLKDDRDSAHLHQSSF-- 66
M+ F LF ++ + S D D+ G +++YIVY+G +++ H S
Sbjct: 1 MAKGTTFIFLFSSLLVLSLSSVSADKDDHGDQQVYIVYLGSLPSREEYTPMSDHMSILQE 60
Query: 67 ----PFAPESVVYTYKRSFNGFAVKLTEEEAEKIAGMEGVVSVFPNKMKQLHTSRSWDFL 126
+V +YK+SFNGFA +LTE E +++AGME VVSVFP++ +L T+ SW+F+
Sbjct: 61 ITGESLIENRLVRSYKKSFNGFAARLTESERKRLAGMERVVSVFPSRKLKLQTTSSWNFM 120
Query: 127 GFPKNV--PRVKQVESNIIVGVLDTGIWPESPSFDDKGFGPIPSKWKGTCQ-GLNFTCN- 186
G + + R + +ES+ I+GV+D+GI+PES SF D+GFGP P KWKGTC G NFTCN
Sbjct: 121 GLKEGIKTKRTRSIESDTIIGVIDSGIYPESDSFSDQGFGPPPKKWKGTCAGGKNFTCNN 180
Query: 187 ----------------SPRDINGHGTHTASTVAGSLVSHASLYGVGLGTARGGVPSARIA 246
+ RD +GHGTHTAS AG+ V++++ YG+G GTARGGVP+ARIA
Sbjct: 181 KVIGARDYTAKSKANQTARDYSGHGTHTASIAAGNAVANSNFYGLGNGTARGGVPAARIA 240
Query: 247 AYKICWNKIGCSQANILAAFDDAIADGVDIISISVGDGDLQPYFLDSVGIGSFHAIQNGI 306
YK+C N+ GC +++AFDDAIADGVD+ISIS+ ++ P+ D + IG+FHA+ G+
Sbjct: 241 VYKVCDNE-GCDGEAMMSAFDDAIADGVDVISISIVLDNIPPFEEDPIAIGAFHAMAVGV 300
Query: 307 LTSNSVGNSGPKLYSTTSVSPWLLSVAASTIDRKIVTQVQIGNKKTFQGVSINIFDMNG- 366
LT N+ GN+GPK+ + TS +PW+ SVAAS +R + +V +G+ K G S+N +DMNG
Sbjct: 301 LTVNAAGNNGPKISTVTSTAPWVFSVAASVTNRAFMAKVVLGDGKILIGRSVNTYDMNGT 360
Query: 367 KYPLVAGHDVLKAGFHNSISRSCRNNSVNPKLVKGKILIC---KELLSRDDFFSFGGAAG 426
YPLV G + +R C ++ KLVKGKI++C K L+ GA G
Sbjct: 361 NYPLVYGKSAALSTCSVDKARLCEPKCLDGKLVKGKIVLCDSTKGLIEAQKL----GAVG 420
Query: 427 VLMVSGTRDTAFPQPLPSSTLDPNDADTIFHYIDSARSPTATIFKSTTLHNVSAPSLASF 486
++ + D AF + P S L +D ++ Y++S ++P AT+ KS + N AP +ASF
Sbjct: 421 SIVKNPEPDRAFIRSFPVSFLSNDDYKSLVSYMNSTKNPKATVLKSEEISNQRAPLVASF 480
Query: 487 SSRGPNVLNKDLVKPDLSAPGVEILAAW-PPVAPVDG---SHRTLYNIVSGTSMACPHIT 546
SSRGP+ + D++KPD++APGVEILAA+ P +P + + R Y+++SGTSMACPH+
Sbjct: 481 SSRGPSSIVSDILKPDITAPGVEILAAYSPDSSPTESEFDTRRVKYSVLSGTSMACPHVA 540
Query: 547 GIAVYVKTFNPTWSPAAIKSALMTTASPMNS--RFHPEAEFAYGSGHVNPLKAVRPGLVY 606
G+A YVKTF+P WSP+ I+SA+MTTA PMN+ EFAYGSGHV+P+ A+ PGLVY
Sbjct: 541 GVAAYVKTFHPQWSPSMIQSAIMTTAWPMNASGSGFVSTEFAYGSGHVDPIDAINPGLVY 600
Query: 607 DANESDYVKFLCGQGYTTNMVRTITNDNSACTSENIGRVW--DLNSPSFGLSVSHSKTFN 666
+ ++D++ FLCG YT++ +R I+ DNS CT E I + +LN P+ VS +K FN
Sbjct: 601 ELTKADHINFLCGLNYTSDHLRIISGDNSTCTKE-ISKTLPRNLNYPTMSAKVSGTKPFN 660
Query: 667 QYFTRTLTSVASQASTYRAMISTPKG--LSITVNPNVLSFNGIGDRKSFKLTVRGTI--R 709
F RT+T+V Q STY A + G LSI V+P VLS + +++SF +TV
Sbjct: 661 ITFQRTVTNVGMQKSTYNAKVVKFPGSKLSIKVSPRVLSMKSMNEKQSFMVTVSSDSIGT 720
BLAST of Lsi04G009180 vs. ExPASy Swiss-Prot
Match:
Q8L7D2 (Subtilisin-like protease SBT4.12 OS=Arabidopsis thaliana OX=3702 GN=SBT4.12 PE=2 SV=1)
HSP 1 Score: 584.7 bits (1506), Expect = 1.4e-165
Identity = 339/736 (46.06%), Postives = 462/736 (62.77%), Query Frame = 0
Query: 3 LSTTMSSSLIFKLLFLNIFFSIQIASSLDSDNDGKKIYIVYMGRKLKDDRD----SAH-- 62
++ +S+ ++ L + + S +++ +D D ++YIVYMG L D S H
Sbjct: 1 MANLAASTCLYSWLLVLLLSS--VSAIIDEDT---QVYIVYMG-SLSSRADYIPTSDHMS 60
Query: 63 -LHQSSFPFAPES-VVYTYKRSFNGFAVKLTEEEAEKIAGMEGVVSVFPNKMKQLHTSRS 122
L Q + + E +V +YKRSFNGFA +LTE E IA +EGVVSVFPNK+ QLHT+ S
Sbjct: 61 ILQQVTGESSIEGRLVRSYKRSFNGFAARLTESERTLIAEIEGVVSVFPNKILQLHTTTS 120
Query: 123 WDFLGFP--KNVPRVKQVESNIIVGVLDTGIWPESPSFDDKGFGPIPSKWKGTCQ-GLNF 182
WDF+G KN R +ES+ I+GV+DTGIWPES SF DKGFGP P KWKG C G NF
Sbjct: 121 WDFMGVKEGKNTKRNLAIESDTIIGVIDTGIWPESKSFSDKGFGPPPKKWKGVCSGGKNF 180
Query: 183 TCNS------------PRDINGHGTHTASTVAGSLVSHASLYGVGLGTARGGVPSARIAA 242
TCN+ RD +GHGTHTAST AG+ V S +G+G GT RGGVP++RIAA
Sbjct: 181 TCNNKLIGARDYTSEGTRDTSGHGTHTASTAAGNAVKDTSFFGIGNGTVRGGVPASRIAA 240
Query: 243 YKICWNKIGCSQANILAAFDDAIADGVDIISISVGDGDLQPYFLDSVGIGSFHAIQNGIL 302
YK+C + GCS +L++FDDAIADGVD+I+IS+G + D + IG+FHA+ GIL
Sbjct: 241 YKVCTDS-GCSSEALLSSFDDAIADGVDLITISIGFQFPSIFEDDPIAIGAFHAMAKGIL 300
Query: 303 TSNSVGNSGPKLYSTTSVSPWLLSVAASTIDRKIVTQVQIGNKKTFQGVSINIFDMNG-K 362
T +S GNSGPK + + V+PW+ +VAAST +R +T+V +GN KT G S+N FDM G K
Sbjct: 301 TVSSAGNSGPKPTTVSHVAPWIFTVAASTTNRGFITKVVLGNGKTLAGRSVNAFDMKGKK 360
Query: 363 YPLVAGHDVLKAGFHNSISRSCRNNSVNPKLVKGKILICKELLSRDDFFSFGGAAGVLMV 422
YPLV G + + C +N VKGKIL+C S GA ++
Sbjct: 361 YPLVYGKSAASSACDAKTAALCAPACLNKSRVKGKILVCGG-PSGYKIAKSVGAIAIIDK 420
Query: 423 SGTRDTAFPQPLPSSTLDPNDADTIFHYIDSARSPTATIFKSTTLHNVSAPSLASFSSRG 482
S D AF LP+S L D ++ YI+S SP A + K+ T+ N ++P +ASFSSRG
Sbjct: 421 SPRPDVAFTHHLPASGLKAKDFKSLVSYIESQDSPQAAVLKTETIFNRTSPVIASFSSRG 480
Query: 483 PNVLNKDLVKPDLSAPGVEILAAWPP--VAPVDGSHRTLYNIVSGTSMACPHITGIAVYV 542
PN + D++KPD++APGVEILAA+ P D + R Y++ SGTSMACPH+ G+A YV
Sbjct: 481 PNTIAVDILKPDITAPGVEILAAFSPNGEPSEDDTRRVKYSVFSGTSMACPHVAGVAAYV 540
Query: 543 KTFNPTWSPAAIKSALMTTASPMNS--RFHPEAEFAYGSGHVNPLKAVRPGLVYDANESD 602
KTF P WSP+ I+SA+MTTA P+ + R EFAYG+GHV+P+ A+ PGLVY+ +++D
Sbjct: 541 KTFYPRWSPSMIQSAIMTTAWPVKAKGRGIASTEFAYGAGHVDPMAALNPGLVYELDKAD 600
Query: 603 YVKFLCGQGYTTNMVRTITNDNSACTSENIGRVWDLNSPSFGLSVSHS-KTFNQYFTRTL 662
++ FLCG YT+ ++ I+ D C+ +N +LN PS +S + TF+ F RTL
Sbjct: 601 HIAFLCGMNYTSKTLKIISGDTVKCSKKNKILPRNLNYPSMSAKLSGTDSTFSVTFNRTL 660
Query: 663 TSVASQASTYRAMISTPKG--LSITVNPNVLSFNGIGDRKSFKLTVRGTIRKDTV--SAF 706
T+V + STY++ + G LSI V P+VL F + +++SF +TV G+ V SA
Sbjct: 661 TNVGTPNSTYKSKVVAGHGSKLSIKVTPSVLYFKTVNEKQSFSVTVTGSDVDSEVPSSAN 720
BLAST of Lsi04G009180 vs. ExPASy TrEMBL
Match:
A0A1S3CFD6 (cucumisin-like OS=Cucumis melo OX=3656 GN=LOC103500254 PE=3 SV=1)
HSP 1 Score: 1021.1 bits (2639), Expect = 2.1e-294
Identity = 525/731 (71.82%), Postives = 593/731 (81.12%), Query Frame = 0
Query: 1 MYLSTTMSSSLIFKLLFLNIFFSIQIASSLDSDNDGKKIYIVYMGRKLKDDRDSAHLHQS 60
M S TMS SLI KL+F N+FF +ASSLDSD+ K+IYIVYMG+K KDD D A+LH S
Sbjct: 1 MRRSNTMSFSLILKLVFFNLFFRTLLASSLDSDD--KEIYIVYMGKKSKDDPDKANLHHS 60
Query: 61 SFPFAPESVVYTYKRSFNGFAVKLTEEEAEKIAGMEGVVSVFPNKMKQLHTSRSWDFLGF 120
SFPFAPESV+YTY RSFNGFAVKLT+EEA+KIA MEGVVSVFPN+M HT+RSWDF+GF
Sbjct: 61 SFPFAPESVLYTYNRSFNGFAVKLTKEEADKIASMEGVVSVFPNEMNTPHTTRSWDFMGF 120
Query: 121 PKNVPRVKQVESNIIVGVLDTGIWPESPSFDDKGFGPIPSKWKGTCQGLNFTCN------ 180
+NVPRVKQVESN++VGVLD+GIWPESPSF+D+GFGP PSKWKGTC +NFTCN
Sbjct: 121 SQNVPRVKQVESNVVVGVLDSGIWPESPSFNDQGFGPPPSKWKGTCSAINFTCNRKIIGA 180
Query: 181 ---------------SPRDINGHGTHTASTVAGSLVSHASLYGVGLGTARGGVPSARIAA 240
PRD NGHGTHTASTVAG LVS ASLYG+GLGTARGGVPSARIA
Sbjct: 181 RSYHIGRPLPLGDVEGPRDTNGHGTHTASTVAGGLVSQASLYGLGLGTARGGVPSARIAV 240
Query: 241 YKICWNKIGCSQANILAAFDDAIADGVDIISISVGDGDLQPYFLDSVGIGSFHAIQNGIL 300
YK+CW + CS A+ILAAFDDAIADGVDIIS+SVG + YF+DS+ IGSFHAI+ GIL
Sbjct: 241 YKVCW-RDACSDADILAAFDDAIADGVDIISLSVGRNVTRKYFIDSIAIGSFHAIEKGIL 300
Query: 301 TSNSVGNSGPKLYSTTSVSPWLLSVAASTIDRKIVTQVQIGNKKTFQGVSINIFDMNGKY 360
TSNS GN+GPKL +T S+SPWLLSVAASTIDRK VT+VQIGN+ +FQG SIN FD G+Y
Sbjct: 301 TSNSAGNNGPKLKTTASLSPWLLSVAASTIDRKFVTKVQIGNQNSFQGFSINTFDNTGQY 360
Query: 361 PLVAGHDVLKAGFHNSISRSCRNNSVNPKLVKGKILICKELLSRDDFFSFGGAAGVLMV- 420
PLV G V GF ++IS C NNSV+ KLVKGKILIC+ F + GG AGVLM+
Sbjct: 361 PLVTGRQVPNTGFDSNISSHCLNNSVDVKLVKGKILICEANFDAKHFVTLGGVAGVLMID 420
Query: 421 SGTRDTAFPQPLPSSTLDPNDADTIFHYIDSARSPTATIFKSTTLHNVSAPSLASFSSRG 480
+ D A P+PS+ LD NDA + YI S SPTATIFKST N AP + SFSSRG
Sbjct: 421 TELIDNARSYPVPSAILDENDAIATYRYIYSNPSPTATIFKSTEQRNEPAPVVVSFSSRG 480
Query: 481 PNVLNKDLVKPDLSAPGVEILAAWPPVAPVDGSHR-TLYNIVSGTSMACPHITGIAVYVK 540
PN + K+++KPDLS PGVEILAAWPPVA V G HR TLYNIVSGTSM+CPHITGIA YVK
Sbjct: 481 PNNITKEIIKPDLSGPGVEILAAWPPVALVGGIHRNTLYNIVSGTSMSCPHITGIAAYVK 540
Query: 541 TFNPTWSPAAIKSALMTTASPMNSRFHPEAEFAYGSGHVNPLKAVRPGLVYDANESDYVK 600
TFNPTWSPAAIKSALMTTA PMN+ + +AEFAYG+GHVNPLKAVRPGLVYDANESDYVK
Sbjct: 541 TFNPTWSPAAIKSALMTTALPMNATLNSDAEFAYGAGHVNPLKAVRPGLVYDANESDYVK 600
Query: 601 FLCGQGYTTNMVRTITNDNSACTSENIGRVWDLNSPSFGLSVSHSKTFNQYFTRTLTSVA 660
FLCGQGYTTNMVR+ITNDNSACT+ NIGRVWDLN PSFGLSVS S+TFNQYFTR LT+VA
Sbjct: 601 FLCGQGYTTNMVRSITNDNSACTASNIGRVWDLNYPSFGLSVSRSQTFNQYFTRILTNVA 660
Query: 661 SQASTYRAMISTPKGLSITVNPNVLSFNGIGDRKSFKLTVRGTIRKDTVSAFLVWSDGVH 709
SQASTYRA IS+P+GL+ITVNP VLSFNGIGDRKSF LTV+GTI++ VSA LVW DGVH
Sbjct: 661 SQASTYRAAISSPQGLTITVNPTVLSFNGIGDRKSFTLTVKGTIKESVVSASLVWFDGVH 720
BLAST of Lsi04G009180 vs. ExPASy TrEMBL
Match:
A0A5A7UD73 (Cucumisin-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold174G001640 PE=3 SV=1)
HSP 1 Score: 1018.5 bits (2632), Expect = 1.4e-293
Identity = 522/725 (72.00%), Postives = 590/725 (81.38%), Query Frame = 0
Query: 7 MSSSLIFKLLFLNIFFSIQIASSLDSDNDGKKIYIVYMGRKLKDDRDSAHLHQSSFPFAP 66
MS SLI KL+F N+FF +ASSLDSD+ K+IYIVYMG+K KDD D A+LH SSFPFAP
Sbjct: 1 MSFSLILKLVFFNLFFRTLLASSLDSDD--KEIYIVYMGKKSKDDPDKANLHHSSFPFAP 60
Query: 67 ESVVYTYKRSFNGFAVKLTEEEAEKIAGMEGVVSVFPNKMKQLHTSRSWDFLGFPKNVPR 126
ESV+YTY RSFNGFAVKLT+EEA+KIA MEGVVSVFPN+M HT+RSWDF+GF +NVPR
Sbjct: 61 ESVLYTYNRSFNGFAVKLTKEEADKIASMEGVVSVFPNEMNTPHTTRSWDFMGFSQNVPR 120
Query: 127 VKQVESNIIVGVLDTGIWPESPSFDDKGFGPIPSKWKGTCQGLNFTCN------------ 186
VKQVESN++VGVLD+GIWPESPSF+D+GFGP PSKWKGTC +NFTCN
Sbjct: 121 VKQVESNVVVGVLDSGIWPESPSFNDQGFGPPPSKWKGTCSAINFTCNRKIIGARSYHIG 180
Query: 187 ---------SPRDINGHGTHTASTVAGSLVSHASLYGVGLGTARGGVPSARIAAYKICWN 246
PRD NGHGTHTASTVAG LVS ASLYG+GLGTARGGVPSARIA YK+CW
Sbjct: 181 RPLPLGDVEGPRDTNGHGTHTASTVAGGLVSQASLYGLGLGTARGGVPSARIAVYKVCW- 240
Query: 247 KIGCSQANILAAFDDAIADGVDIISISVGDGDLQPYFLDSVGIGSFHAIQNGILTSNSVG 306
+ CS A+ILAAFDDAIADGVDIIS+SVG + YF+DS+ IGSFHAI+ GILTSNS G
Sbjct: 241 RDACSDADILAAFDDAIADGVDIISLSVGRNVTRKYFIDSIAIGSFHAIEKGILTSNSAG 300
Query: 307 NSGPKLYSTTSVSPWLLSVAASTIDRKIVTQVQIGNKKTFQGVSINIFDMNGKYPLVAGH 366
N+GPKL +T S+SPWLLSVAASTIDRK VT+VQIGN+ +FQG SIN FD G+YPLV G
Sbjct: 301 NNGPKLKTTASLSPWLLSVAASTIDRKFVTKVQIGNQNSFQGFSINTFDNTGQYPLVTGR 360
Query: 367 DVLKAGFHNSISRSCRNNSVNPKLVKGKILICKELLSRDDFFSFGGAAGVLMV-SGTRDT 426
V GF ++IS C NNSV+ KLVKGKILIC+ F + GG AGVLM+ + D
Sbjct: 361 QVPNTGFDSNISSHCLNNSVDVKLVKGKILICEANFDAKHFVTLGGVAGVLMIDTELIDN 420
Query: 427 AFPQPLPSSTLDPNDADTIFHYIDSARSPTATIFKSTTLHNVSAPSLASFSSRGPNVLNK 486
A P+PS+ LD NDA + YI S SPTATIFKST N AP + SFSSRGPN + K
Sbjct: 421 ARSYPVPSAILDENDAIATYRYIYSNPSPTATIFKSTEQRNEPAPVVVSFSSRGPNNITK 480
Query: 487 DLVKPDLSAPGVEILAAWPPVAPVDGSHR-TLYNIVSGTSMACPHITGIAVYVKTFNPTW 546
+++KPDLS PGVEILAAWPPVA V G HR TLYNIVSGTSM+CPHITGIA YVKTFNPTW
Sbjct: 481 EIIKPDLSGPGVEILAAWPPVALVGGIHRNTLYNIVSGTSMSCPHITGIAAYVKTFNPTW 540
Query: 547 SPAAIKSALMTTASPMNSRFHPEAEFAYGSGHVNPLKAVRPGLVYDANESDYVKFLCGQG 606
SPAAIKSALMTTA PMN+ + +AEFAYG+GHVNPLKAVRPGLVYDANESDYVKFLCGQG
Sbjct: 541 SPAAIKSALMTTALPMNATLNSDAEFAYGAGHVNPLKAVRPGLVYDANESDYVKFLCGQG 600
Query: 607 YTTNMVRTITNDNSACTSENIGRVWDLNSPSFGLSVSHSKTFNQYFTRTLTSVASQASTY 666
YTTNMVR+ITNDNSACT+ NIGRVWDLN PSFGLSVS S+TFNQYFTR LT+VASQASTY
Sbjct: 601 YTTNMVRSITNDNSACTASNIGRVWDLNYPSFGLSVSRSQTFNQYFTRILTNVASQASTY 660
Query: 667 RAMISTPKGLSITVNPNVLSFNGIGDRKSFKLTVRGTIRKDTVSAFLVWSDGVHTVRSPI 709
RA IS+P+GL+ITVNP VLSFNGIGDRKSF LTV+GTI++ VSA LVW DGVH+VRSPI
Sbjct: 661 RAAISSPQGLTITVNPTVLSFNGIGDRKSFTLTVKGTIKESVVSASLVWFDGVHSVRSPI 720
BLAST of Lsi04G009180 vs. ExPASy TrEMBL
Match:
A0A1S3CF86 (LOW QUALITY PROTEIN: cucumisin OS=Cucumis melo OX=3656 GN=LOC103500253 PE=3 SV=1)
HSP 1 Score: 1007.3 bits (2603), Expect = 3.2e-290
Identity = 525/733 (71.62%), Postives = 586/733 (79.95%), Query Frame = 0
Query: 7 MSSSLIFKLLFLNIFFSIQIASSLDSDNDGKKIYIVYMGRKLKDDRDSAHLHQSSF---- 66
MSSSLIFKL F ++FFS ++AS LDSD+DGK IYIVYMGRKL +D DSAHLH +
Sbjct: 1 MSSSLIFKLFFFSLFFSNRLASRLDSDDDGKNIYIVYMGRKL-EDPDSAHLHHRAMLEQV 60
Query: 67 ---PFAPESVVYTYKRSFNGFAVKLTEEEAEKIAGMEGVVSVFPNKMKQLHTSRSWDFLG 126
FAPESV++TYKRSFNGFAVKLTEEEAEKIA MEGVVSVF N+M +LHT+RSWDFLG
Sbjct: 61 VGSTFAPESVLHTYKRSFNGFAVKLTEEEAEKIASMEGVVSVFLNEMNELHTTRSWDFLG 120
Query: 127 FPKNVPRVKQVESNIIVGVLDTGIWPESPSFDDKGFGPIPSKWKGTCQ-GLNFTC----- 186
FP VPR QVESNI+VGVLDTGIWPESPSFDD+GF P P KWKGTC+ NF C
Sbjct: 121 FPLTVPRRSQVESNIVVGVLDTGIWPESPSFDDEGFSPPPPKWKGTCETSNNFRCNRKII 180
Query: 187 ----------------NSPRDINGHGTHTASTVAGSLVSHASLYGVGLGTARGGVPSARI 246
N PRD NGHGTHTAST AG LVS A+LYG+GLGTARGGVP ARI
Sbjct: 181 GARSYHIGRPISPGDVNGPRDTNGHGTHTASTAAGGLVSQANLYGLGLGTARGGVPLARI 240
Query: 247 AAYKICWNKIGCSQANILAAFDDAIADGVDIISISVGDGDLQPYFLDSVGIGSFHAIQNG 306
AAYK+CWN GCS A+ILAA+DDAIADGVDIIS+SVG + + YF+D++ IGSFHA++ G
Sbjct: 241 AAYKVCWND-GCSDADILAAYDDAIADGVDIISLSVGGANPRHYFVDAIAIGSFHAVERG 300
Query: 307 ILTSNSVGNSGPKLYSTTSVSPWLLSVAASTIDRKIVTQVQIGNKKTFQGVSINIFDMNG 366
ILTSNS GN GP ++T S+SPWLLSVAAST+DRK VTQVQIGN ++FQGVSIN FD N
Sbjct: 301 ILTSNSAGNGGPNFFTTASLSPWLLSVAASTMDRKFVTQVQIGNGQSFQGVSINTFD-NQ 360
Query: 367 KYPLVAGHDVLKAGFHNSISRSCRNNSVNPKLVKGKILICKELLSRDDFF-SFGGAAGVL 426
YPLV+G D+ GF S SR C +NSV PKL+KGKI++C+ +FF S GAAGVL
Sbjct: 361 YYPLVSGRDIPNPGFDKSTSRFCTDNSVXPKLLKGKIVVCEASFGPHEFFKSLDGAAGVL 420
Query: 427 MVSGTRDTAFPQPLPSSTLDPNDADTIFHYIDSARSPTATIFKSTTLHNVSAPSLASFSS 486
M S TRD A PLPSS LDPND YI S RSP ATIFKSTT+ N SAP + SFSS
Sbjct: 421 MTSNTRDYADSYPLPSSVLDPNDLLATLRYIYSIRSPGATIFKSTTILNASAPVVVSFSS 480
Query: 487 RGPNVLNKDLVKPDLSAPGVEILAAWPPVAPVDGSHR-TLYNIVSGTSMACPHITGIAVY 546
RGPN KD++KPD+S PGVEILAAWP VAPV G R TL+NI+SGTSM+CPHITGIA Y
Sbjct: 481 RGPNRATKDVIKPDISGPGVEILAAWPSVAPVGGIRRNTLFNIISGTSMSCPHITGIATY 540
Query: 547 VKTFNPTWSPAAIKSALMTTASPMNSRFHPEAEFAYGSGHVNPLKAVRPGLVYDANESDY 606
VKT+NPTWSPAAIKSALMTTASPMN+RF+P+AEFAYGSGHVNPLKAVRPGLVYDANESDY
Sbjct: 541 VKTYNPTWSPAAIKSALMTTASPMNARFNPQAEFAYGSGHVNPLKAVRPGLVYDANESDY 600
Query: 607 VKFLCGQGYTTNMVRTITNDNSACTSENIGRVWDLNSPSFGLSVSHSKTFNQYFTRTLTS 666
VKFLCGQGY T VR IT D SACT N GRVWDLN PSFGLSVS SKTFNQYF RTLTS
Sbjct: 601 VKFLCGQGYNTEAVRRITGDYSACTPGNTGRVWDLNYPSFGLSVSPSKTFNQYFNRTLTS 660
Query: 667 VASQASTYRAMISTPKGLSITVNPNVLSFNGIGDRKSFKLTVRGTIRKDTVSAFLVWSDG 709
VA QASTYRAMIS P+GL+I+VNPNVLSFNG+GDRKSF LTVRG+I+ VSA LVWSDG
Sbjct: 661 VAPQASTYRAMISAPQGLTISVNPNVLSFNGLGDRKSFTLTVRGSIKGFVVSASLVWSDG 720
BLAST of Lsi04G009180 vs. ExPASy TrEMBL
Match:
A0A1S4E3D9 (LOW QUALITY PROTEIN: cucumisin-like OS=Cucumis melo OX=3656 GN=LOC103500257 PE=3 SV=1)
HSP 1 Score: 984.9 bits (2545), Expect = 1.7e-283
Identity = 509/731 (69.63%), Postives = 576/731 (78.80%), Query Frame = 0
Query: 1 MYLSTTMSSSLIFKLLFLNIFFSIQIASSLDSDNDGKKIYIVYMGRKLKDDRDSAHLHQS 60
M S TMS SL+F L+FLN+FFS +AS+LDSD++G+KIYIVYMG+KLKDD DSA+LH S
Sbjct: 1 MTRSNTMSFSLLFNLVFLNLFFSTLLASTLDSDDNGRKIYIVYMGKKLKDDPDSANLHHS 60
Query: 61 SFPFAPESVVYTYKRSFNGFAVKLTEEEAEKIAGMEGVVSVFPNKMKQLHTSRSWDFLGF 120
SFPFAPESV+YTY RSFNGFAVKLT+EEA+KIAGM+GVVSVFPN++ +LHT+RSWDF+GF
Sbjct: 61 SFPFAPESVIYTYNRSFNGFAVKLTKEEADKIAGMKGVVSVFPNEINKLHTTRSWDFMGF 120
Query: 121 PKNVPRVKQVESNIIVGVLDTGIWPESPSFDDKGFGPIPSKWKGTCQGLNFTCN------ 180
P+NV RVKQV SNI+VGV D+GIWPESPSF+DKGF P PSKWKGTC NFTCN
Sbjct: 121 PQNVRRVKQVGSNIVVGVFDSGIWPESPSFNDKGFDPPPSKWKGTCSAFNFTCNRKIIGA 180
Query: 181 ---------------SPRDINGHGTHTASTVAGSLVSHASLYGVGLGTARGGVPSARIAA 240
PRD +GHGTH AS G LV+ ASL G+GLGTARGG+PSARIA
Sbjct: 181 RAYHIGRPLPHGDVEGPRDTDGHGTHCASIAPGGLVNKASLNGLGLGTARGGIPSARIAV 240
Query: 241 YKICWNKIGCSQANILAAFDDAIADGVDIISISVGDGDLQPYFLDSVGIGSFHAIQNGIL 300
YKICWN ++LAAFDDAI+DGVDIIS+SVG + YF D + IGSFHAIQN IL
Sbjct: 241 YKICWND---DXMDLLAAFDDAISDGVDIISLSVGGNISRKYFRDPIAIGSFHAIQNNIL 300
Query: 301 TSNSVGNSGPKLYSTTSVSPWLLSVAASTIDRKIVTQVQIGNKKTFQGVSINIFDMNGKY 360
TSNS GN GP +Y+ TS+SPWLLSVAAST+DRK VT+VQIGNK++ QGVSIN F G+Y
Sbjct: 301 TSNSAGNWGPNVYTVTSLSPWLLSVAASTMDRKFVTKVQIGNKRSIQGVSINTFGTTGQY 360
Query: 361 PLVAGHDVLKAGFHNSISRSCRNNSVNPKLVKGKILICKELLSRDDFFSFGGAAGVLMVS 420
PLVA DV GF N S C NNSVN KLVKGKIL C+ F SFGG AGVLMV+
Sbjct: 361 PLVAARDVPNNGFDNLTSTYCFNNSVNLKLVKGKILFCESSFHPVLFSSFGGVAGVLMVN 420
Query: 421 -GTRDTAFPQPLPSSTLDPNDADTIFHYIDSARSPTATIFKSTTLHNVSAPSLASFSSRG 480
D A PLPSS L+ +DA TIF YI S RSP A+I +ST + N AP + SFSSRG
Sbjct: 421 VNPLDDALSFPLPSSVLNFHDAITIFDYIRSTRSPNASILRSTAVRNEPAPVVVSFSSRG 480
Query: 481 PNVLNKDLVKPDLSAPGVEILAAWPPVAPVDGSHR-TLYNIVSGTSMACPHITGIAVYVK 540
PN L K+++KPDLS PGVEILAAWPPVAPV +R TLYNI+SGTSM+CPHIT IA YVK
Sbjct: 481 PNNLTKEIIKPDLSGPGVEILAAWPPVAPVGEINRNTLYNIISGTSMSCPHITEIAAYVK 540
Query: 541 TFNPTWSPAAIKSALMTTASPMNSRFHPEAEFAYGSGHVNPLKAVRPGLVYDANESDYVK 600
TFNPTWSPAAIKSALMTTA PMN+ + EAEFAYGSGHVNP +A+RPGLVYDANE DY+K
Sbjct: 541 TFNPTWSPAAIKSALMTTALPMNATLNLEAEFAYGSGHVNPKRAIRPGLVYDANEIDYIK 600
Query: 601 FLCGQGYTTNMVRTITNDNSACTSENIGRVWDLNSPSFGLSVSHSKTFNQYFTRTLTSVA 660
FLCGQGYT MV IT+ AC S NIGRVWDLN PSFGLSVSHSKTF QYF RTLTSVA
Sbjct: 601 FLCGQGYTNGMVEIITSYEDACNSSNIGRVWDLNYPSFGLSVSHSKTFKQYFRRTLTSVA 660
Query: 661 SQASTYRAMISTPKGLSITVNPNVLSFNGIGDRKSFKLTVRGTIRKDTVSAFLVWSDGVH 709
SQAS Y+AMIS P+GL ITVNPNVLSFNGIGD+KSFKL VRGTI++ VSA LVWSDGVH
Sbjct: 661 SQASKYKAMISAPRGLVITVNPNVLSFNGIGDKKSFKLKVRGTIKESIVSASLVWSDGVH 720
BLAST of Lsi04G009180 vs. ExPASy TrEMBL
Match:
A0A5D3E4N6 (Cucumisin-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold267G00100 PE=3 SV=1)
HSP 1 Score: 977.2 bits (2525), Expect = 3.5e-281
Identity = 508/725 (70.07%), Postives = 578/725 (79.72%), Query Frame = 0
Query: 7 MSSSLIFKLLFLNIFFSIQIASSLDSDNDGKKIYIVYMGRKLKDDRDSAHLHQSSFPFAP 66
MS SLI KL+F N+FF +ASSLDSD+ K+IYIVYMG+K KDD D A+LH SSFPFAP
Sbjct: 1 MSFSLILKLVFFNLFFRTLLASSLDSDD--KEIYIVYMGKKSKDDPDKANLHHSSFPFAP 60
Query: 67 ESVVYTYKRSFNGFAVKLTEEEAEKIAGMEGVVSVFPNKMKQLHTSRSWDFLGFPKNVPR 126
ESV+YTY RSFNGFAVKLT+EEA+KIA MEGVVSVFPN+M HT+RSWDF+GF +NVPR
Sbjct: 61 ESVLYTYNRSFNGFAVKLTKEEADKIASMEGVVSVFPNEMNTPHTTRSWDFMGFSQNVPR 120
Query: 127 VKQVESNIIVGVLDTGIWPESPSFDDKGFGPIPSKWKGTCQGLNFTCN------------ 186
VKQVESN++VGVLD+GIWPESPSF+D+GFGP PSKWKGTC +NFTCN
Sbjct: 121 VKQVESNVVVGVLDSGIWPESPSFNDQGFGPPPSKWKGTCSAINFTCNRKIIGARSYHIG 180
Query: 187 ---------SPRDINGHGTHTASTVAGSLVSHASLYGVGLGTARGGVPSARIAAYKICWN 246
PRD NGHGTHTASTVAG LVS ASLYG+GLGTARGGVPSARIA YK+CW
Sbjct: 181 RPLPLGDVEGPRDTNGHGTHTASTVAGGLVSQASLYGLGLGTARGGVPSARIAVYKVCW- 240
Query: 247 KIGCSQANILAAFDDAIADGVDIISISVGDGDLQPYFLDSVGIGSFHAIQNGILTSNSVG 306
+ CS A+ILAAFDDAIADGVDIIS+SVG + YF+DS+ IGSFHAI+ GILTSNS G
Sbjct: 241 RDACSDADILAAFDDAIADGVDIISLSVGRNVTRKYFIDSIAIGSFHAIEKGILTSNSAG 300
Query: 307 NSGPKLYSTTSVSPWLLSVAASTIDRKIVTQVQIGNKKTFQGVSINIFDMNGKYPLVAGH 366
N+GPKL +T S+SPWLLSVAASTIDRK VT+VQIGN+ +FQ + + F +
Sbjct: 301 NNGPKLKTTASLSPWLLSVAASTIDRKFVTKVQIGNQNSFQVILLIFF--------ILFF 360
Query: 367 DVLKAGFHNSISRSCRNNSVNPKLVKGKILICKELLSRDDFFSFGGAAGVLMV-SGTRDT 426
L F + + C NNSV+ KLVKGKILIC+ F + GG AGVLM+ + D
Sbjct: 361 SKLYLIFVKTWNSHCLNNSVDVKLVKGKILICEANFDAKHFVTLGGVAGVLMIDTELIDN 420
Query: 427 AFPQPLPSSTLDPNDADTIFHYIDSARSPTATIFKSTTLHNVSAPSLASFSSRGPNVLNK 486
A P+PS+ LD NDA + YI S SPTATIFKST N AP + SFSSRGPN + K
Sbjct: 421 ARSYPVPSAILDENDAIATYRYIYSNPSPTATIFKSTEQRNEPAPVVVSFSSRGPNNITK 480
Query: 487 DLVKPDLSAPGVEILAAWPPVAPVDGSHR-TLYNIVSGTSMACPHITGIAVYVKTFNPTW 546
+++KPDLS PGVEILAAWPPVA V G HR TLYNIVSGTSM+CPHITGIA YVKTFNPTW
Sbjct: 481 EIIKPDLSGPGVEILAAWPPVALVGGIHRNTLYNIVSGTSMSCPHITGIAAYVKTFNPTW 540
Query: 547 SPAAIKSALMTTASPMNSRFHPEAEFAYGSGHVNPLKAVRPGLVYDANESDYVKFLCGQG 606
SPAAIKSALMTTA PMN+ + +AEFAYG+GHVNPLKAVRPGLVYDANESDYVKFLCGQG
Sbjct: 541 SPAAIKSALMTTALPMNATLNSDAEFAYGAGHVNPLKAVRPGLVYDANESDYVKFLCGQG 600
Query: 607 YTTNMVRTITNDNSACTSENIGRVWDLNSPSFGLSVSHSKTFNQYFTRTLTSVASQASTY 666
YTTNMVR+ITNDNSACT+ NIGRVWDLN PSFGLSVS S+TFNQYFTR LT+VASQASTY
Sbjct: 601 YTTNMVRSITNDNSACTASNIGRVWDLNYPSFGLSVSRSQTFNQYFTRILTNVASQASTY 660
Query: 667 RAMISTPKGLSITVNPNVLSFNGIGDRKSFKLTVRGTIRKDTVSAFLVWSDGVHTVRSPI 709
RA IS+P+GL+ITVNP VLSFNGIGDRKSF LTV+GTI++ VSA LVW DGVH+VRSPI
Sbjct: 661 RAAISSPQGLTITVNPTVLSFNGIGDRKSFTLTVKGTIKESVVSASLVWFDGVHSVRSPI 714
BLAST of Lsi04G009180 vs. NCBI nr
Match:
XP_038892403.1 (cucumisin-like isoform X1 [Benincasa hispida])
HSP 1 Score: 1070.5 bits (2767), Expect = 6.5e-309
Identity = 547/732 (74.73%), Postives = 600/732 (81.97%), Query Frame = 0
Query: 1 MYLSTTMSSSLIFKLLFLNIFFSIQIASSLDSDNDGKKIYIVYMGRKLKDDRDSAHLHQS 60
M ST M LIFKL FL++FFS +ASSLDSD+D KKIYIVYMGRK+KDD DSAHLH S
Sbjct: 1 MTRSTAMYFCLIFKLFFLSLFFSTLLASSLDSDDD-KKIYIVYMGRKIKDDPDSAHLHHS 60
Query: 61 SFPFAPESVVYTYKRSFNGFAVKLTEEEAEKIAGMEGVVSVFPNKMKQLHTSRSWDFLGF 120
SFPFAPESVVY YKRSFNGFAVKLT+EEAEKIA MEGVVSVFPNK+ +LHT+RSWDF+ F
Sbjct: 61 SFPFAPESVVYMYKRSFNGFAVKLTKEEAEKIASMEGVVSVFPNKINKLHTTRSWDFMNF 120
Query: 121 PKNVPRVKQVESNIIVGVLDTGIWPESPSFDDKGFGPIPSKWKGTCQGLNFTCN------ 180
PKNVPRVKQVESNI+VGV DTGIWPESPSF+DKGFGP PSKWKGTC NFTCN
Sbjct: 121 PKNVPRVKQVESNIVVGVFDTGIWPESPSFNDKGFGPPPSKWKGTCLVFNFTCNRKIIGA 180
Query: 181 ---------------SPRDINGHGTHTASTVAGSLVSHASLYGVGLGTARGGVPSARIAA 240
SPRD NGHGTHTAST AG LVS ASLYG+GLGTARGGVPSARIAA
Sbjct: 181 RAYHIGRPLPRGEVESPRDTNGHGTHTASTAAGGLVSKASLYGLGLGTARGGVPSARIAA 240
Query: 241 YKICWNKIGCSQANILAAFDDAIADGVDIISISVGDGDLQPYFLDSVGIGSFHAIQNGIL 300
YKICW+ CS +ILAAFDDAIADGVDIIS+SVG + + YF D + IGSFHA+Q GIL
Sbjct: 241 YKICWSD-SCSDLDILAAFDDAIADGVDIISLSVGGNESRQYFRDPIAIGSFHAMQKGIL 300
Query: 301 TSNSVGNSGPKLYSTTSVSPWLLSVAASTIDRKIVTQVQIGNKKTFQGVSINIFDMNGKY 360
TSNS GN GP+ ++TTS+SPWLLSVAAST DRK VT+VQIGNK +FQGVSIN FD G+Y
Sbjct: 301 TSNSAGNDGPRYFTTTSLSPWLLSVAASTTDRKFVTKVQIGNKNSFQGVSINTFDTKGQY 360
Query: 361 PLVAGHDVLKAGFHNSISRSCRNNSVNPKLVKGKILICKELLSRDDFFSFGGAAGVLMV- 420
PLVAG D+ GFHNS SR C NNSV+PKLVKGKI+ C+ + +F S GGA GVL
Sbjct: 361 PLVAGRDIPNKGFHNSTSRYCFNNSVDPKLVKGKIVFCETNVDSSEFISLGGAMGVLGKG 420
Query: 421 -SGTRDTAFPQPLPSSTLDPNDADTIFHYIDSARSPTATIFKSTTLHNVSAPSLASFSSR 480
T D F PLPSSTLD +DA I HYID+ R PTATIFKST HN +P + SFSSR
Sbjct: 421 DKNTMDCEFSYPLPSSTLDVDDATIISHYIDNTRFPTATIFKSTATHNAPSPVVVSFSSR 480
Query: 481 GPNVLNKDLVKPDLSAPGVEILAAWPPVAPVDGSHR-TLYNIVSGTSMACPHITGIAVYV 540
GPN KDL+KPDLSAPGVEILAAWPPVAPV G R TLYNI+SGTSM+CPH+TGIA YV
Sbjct: 481 GPNAATKDLIKPDLSAPGVEILAAWPPVAPVGGILRDTLYNIISGTSMSCPHVTGIAAYV 540
Query: 541 KTFNPTWSPAAIKSALMTTASPMNSRFHPEAEFAYGSGHVNPLKAVRPGLVYDANESDYV 600
KTFNPTWSPAAIKSALMTTASPMNS+F+P+AEFAYGSGHVNPLKA+ PGLVYDANE+DYV
Sbjct: 541 KTFNPTWSPAAIKSALMTTASPMNSQFNPQAEFAYGSGHVNPLKALTPGLVYDANETDYV 600
Query: 601 KFLCGQGYTTNMVRTITNDNSACTSENIGRVWDLNSPSFGLSVSHSKTFNQYFTRTLTSV 660
KFLCGQGYTT++VR ITNDNS C S N GRVWDLN PSFGLSVSHSKTFNQYFTRTLTSV
Sbjct: 601 KFLCGQGYTTDLVRIITNDNSVCISTNTGRVWDLNYPSFGLSVSHSKTFNQYFTRTLTSV 660
Query: 661 ASQASTYRAMISTPKGLSITVNPNVLSFNGIGDRKSFKLTVRGTIRKDTVSAFLVWSDGV 709
AS ASTY+AMIS PKGL+ITV P VLSFNG GD KSFKLTVRGT+R+ VSA L+WSD V
Sbjct: 661 ASGASTYKAMISAPKGLAITVKPKVLSFNGTGDMKSFKLTVRGTMRESIVSASLIWSDSV 720
BLAST of Lsi04G009180 vs. NCBI nr
Match:
XP_038892506.1 (cucumisin-like [Benincasa hispida])
HSP 1 Score: 1030.8 bits (2664), Expect = 5.6e-297
Identity = 531/738 (71.95%), Postives = 597/738 (80.89%), Query Frame = 0
Query: 1 MYLSTTMSSSLIFKLLFLNIFFSIQIASSLDSDNDGKKIYIVYMGRKLKDDRDSAHLHQS 60
M ST MSSSL+FKL F++ FFS + SS +SD+DGKKIYIVYMGRKL +D DSAHLH
Sbjct: 1 MIRSTAMSSSLVFKLFFVSFFFSTILTSSFESDDDGKKIYIVYMGRKL-EDPDSAHLHHR 60
Query: 61 SF-------PFAPESVVYTYKRSFNGFAVKLTEEEAEKIAGMEGVVSVFPNKMKQLHTSR 120
+ FAPESV+Y+YKRSFNGF VKLTEEEAEKIA MEGVVSVF N+M LHT+R
Sbjct: 61 AMLEQVVGSNFAPESVLYSYKRSFNGFVVKLTEEEAEKIASMEGVVSVFLNEMNVLHTTR 120
Query: 121 SWDFLGFPKNVPRVKQVESNIIVGVLDTGIWPESPSFDDKGFGPIPSKWKGTCQGLNFTC 180
SWDFL FP+N+ RV QVESNI+VGVLD+GIWPESPSF+DKGF PSKWKG+CQ NFTC
Sbjct: 121 SWDFLNFPQNIQRVNQVESNIVVGVLDSGIWPESPSFNDKGFDSPPSKWKGSCQAFNFTC 180
Query: 181 ---------------------NSPRDINGHGTHTASTVAGSLVSHASLYGVGLGTARGGV 240
NSPRD +GHGTHTAST AG LVS A+LY +GLGTARGGV
Sbjct: 181 NRKIIGGRAYHIGGPLRPGDVNSPRDTDGHGTHTASTAAGGLVSQANLYRLGLGTARGGV 240
Query: 241 PSARIAAYKICWNKIGCSQANILAAFDDAIADGVDIISISVGDGDLQPYFLDSVGIGSFH 300
P ARIA YKICW K GCS A+ILAA+DDAIADGVDIIS+SVG + YF D + IGSFH
Sbjct: 241 PLARIAVYKICW-KDGCSDADILAAYDDAIADGVDIISLSVGANKPRQYFSDPIAIGSFH 300
Query: 301 AIQNGILTSNSVGNSGPKLYSTTSVSPWLLSVAASTIDRKIVTQVQIGNKKTFQGVSINI 360
AI+ GILTSNS GN GP ++TTS+SPWLLSVAASTIDRK VTQVQIGN ++FQGVSIN
Sbjct: 301 AIEKGILTSNSAGNEGPNFFTTTSLSPWLLSVAASTIDRKFVTQVQIGNGQSFQGVSINT 360
Query: 361 FDMNGKYPLVAGHDVLKAGFHNSISRSCRNNSVNPKLVKGKILICKELLSRDDFF-SFGG 420
F+MNG+YPLV G D+ GF +S SR C N SVNP L++GKI++C+ +FF S G
Sbjct: 361 FEMNGQYPLVLGRDIPNIGFDSSTSRYCFNKSVNPYLLRGKIVLCEASFGPAEFFKSLDG 420
Query: 421 AAGVLMVSGTRDTAFPQPLPSSTLDPNDADTIFHYIDSARSPTATIFKSTTLHNVSAPSL 480
AAGVLM++ TRD A P PSSTLDPNDA IF YI S PTATIFKST + N SAP +
Sbjct: 421 AAGVLMLASTRDHASSYPFPSSTLDPNDATDIFRYIYSTSFPTATIFKSTAILNTSAPVV 480
Query: 481 ASFSSRGPNVLNKDLVKPDLSAPGVEILAAWPPVAPVDGSHR-TLYNIVSGTSMACPHIT 540
SFSSRGPN KDL+KPDLSAPGVEILAAWPPVAPV G R TLYNI+SGTSM+CPH+T
Sbjct: 481 VSFSSRGPNAATKDLIKPDLSAPGVEILAAWPPVAPVGGILRDTLYNIISGTSMSCPHVT 540
Query: 541 GIAVYVKTFNPTWSPAAIKSALMTTASPMNSRFHPEAEFAYGSGHVNPLKAVRPGLVYDA 600
GIA YVKTFNPTWSPAAIKSALMTTASPMN+RFHP+ EFAYGSGHVNPLKAVRPGLVYDA
Sbjct: 541 GIAAYVKTFNPTWSPAAIKSALMTTASPMNARFHPQGEFAYGSGHVNPLKAVRPGLVYDA 600
Query: 601 NESDYVKFLCGQGYTTNMVRTITNDNSACTSENIGRVWDLNSPSFGLSVSHSKTFNQYFT 660
NESDYVKFLCGQGY+T+MVR IT D SACTS NIGRVWDLN PSFGLSVS S+TF+QYFT
Sbjct: 601 NESDYVKFLCGQGYSTSMVRRITGDYSACTSGNIGRVWDLNYPSFGLSVSGSQTFSQYFT 660
Query: 661 RTLTSVASQASTYRAMISTPKGLSITVNPNVLSFNGIGDRKSFKLTVRGTIRKDTVSAFL 709
RTLTSVASQASTYRAMIS P+GL+ITVNPNVLSFNGIGD+KSF LT+RG++ + VSA L
Sbjct: 661 RTLTSVASQASTYRAMISAPQGLAITVNPNVLSFNGIGDKKSFTLTIRGSVNQYVVSASL 720
BLAST of Lsi04G009180 vs. NCBI nr
Match:
XP_008461722.1 (PREDICTED: cucumisin-like [Cucumis melo])
HSP 1 Score: 1021.1 bits (2639), Expect = 4.4e-294
Identity = 525/731 (71.82%), Postives = 593/731 (81.12%), Query Frame = 0
Query: 1 MYLSTTMSSSLIFKLLFLNIFFSIQIASSLDSDNDGKKIYIVYMGRKLKDDRDSAHLHQS 60
M S TMS SLI KL+F N+FF +ASSLDSD+ K+IYIVYMG+K KDD D A+LH S
Sbjct: 1 MRRSNTMSFSLILKLVFFNLFFRTLLASSLDSDD--KEIYIVYMGKKSKDDPDKANLHHS 60
Query: 61 SFPFAPESVVYTYKRSFNGFAVKLTEEEAEKIAGMEGVVSVFPNKMKQLHTSRSWDFLGF 120
SFPFAPESV+YTY RSFNGFAVKLT+EEA+KIA MEGVVSVFPN+M HT+RSWDF+GF
Sbjct: 61 SFPFAPESVLYTYNRSFNGFAVKLTKEEADKIASMEGVVSVFPNEMNTPHTTRSWDFMGF 120
Query: 121 PKNVPRVKQVESNIIVGVLDTGIWPESPSFDDKGFGPIPSKWKGTCQGLNFTCN------ 180
+NVPRVKQVESN++VGVLD+GIWPESPSF+D+GFGP PSKWKGTC +NFTCN
Sbjct: 121 SQNVPRVKQVESNVVVGVLDSGIWPESPSFNDQGFGPPPSKWKGTCSAINFTCNRKIIGA 180
Query: 181 ---------------SPRDINGHGTHTASTVAGSLVSHASLYGVGLGTARGGVPSARIAA 240
PRD NGHGTHTASTVAG LVS ASLYG+GLGTARGGVPSARIA
Sbjct: 181 RSYHIGRPLPLGDVEGPRDTNGHGTHTASTVAGGLVSQASLYGLGLGTARGGVPSARIAV 240
Query: 241 YKICWNKIGCSQANILAAFDDAIADGVDIISISVGDGDLQPYFLDSVGIGSFHAIQNGIL 300
YK+CW + CS A+ILAAFDDAIADGVDIIS+SVG + YF+DS+ IGSFHAI+ GIL
Sbjct: 241 YKVCW-RDACSDADILAAFDDAIADGVDIISLSVGRNVTRKYFIDSIAIGSFHAIEKGIL 300
Query: 301 TSNSVGNSGPKLYSTTSVSPWLLSVAASTIDRKIVTQVQIGNKKTFQGVSINIFDMNGKY 360
TSNS GN+GPKL +T S+SPWLLSVAASTIDRK VT+VQIGN+ +FQG SIN FD G+Y
Sbjct: 301 TSNSAGNNGPKLKTTASLSPWLLSVAASTIDRKFVTKVQIGNQNSFQGFSINTFDNTGQY 360
Query: 361 PLVAGHDVLKAGFHNSISRSCRNNSVNPKLVKGKILICKELLSRDDFFSFGGAAGVLMV- 420
PLV G V GF ++IS C NNSV+ KLVKGKILIC+ F + GG AGVLM+
Sbjct: 361 PLVTGRQVPNTGFDSNISSHCLNNSVDVKLVKGKILICEANFDAKHFVTLGGVAGVLMID 420
Query: 421 SGTRDTAFPQPLPSSTLDPNDADTIFHYIDSARSPTATIFKSTTLHNVSAPSLASFSSRG 480
+ D A P+PS+ LD NDA + YI S SPTATIFKST N AP + SFSSRG
Sbjct: 421 TELIDNARSYPVPSAILDENDAIATYRYIYSNPSPTATIFKSTEQRNEPAPVVVSFSSRG 480
Query: 481 PNVLNKDLVKPDLSAPGVEILAAWPPVAPVDGSHR-TLYNIVSGTSMACPHITGIAVYVK 540
PN + K+++KPDLS PGVEILAAWPPVA V G HR TLYNIVSGTSM+CPHITGIA YVK
Sbjct: 481 PNNITKEIIKPDLSGPGVEILAAWPPVALVGGIHRNTLYNIVSGTSMSCPHITGIAAYVK 540
Query: 541 TFNPTWSPAAIKSALMTTASPMNSRFHPEAEFAYGSGHVNPLKAVRPGLVYDANESDYVK 600
TFNPTWSPAAIKSALMTTA PMN+ + +AEFAYG+GHVNPLKAVRPGLVYDANESDYVK
Sbjct: 541 TFNPTWSPAAIKSALMTTALPMNATLNSDAEFAYGAGHVNPLKAVRPGLVYDANESDYVK 600
Query: 601 FLCGQGYTTNMVRTITNDNSACTSENIGRVWDLNSPSFGLSVSHSKTFNQYFTRTLTSVA 660
FLCGQGYTTNMVR+ITNDNSACT+ NIGRVWDLN PSFGLSVS S+TFNQYFTR LT+VA
Sbjct: 601 FLCGQGYTTNMVRSITNDNSACTASNIGRVWDLNYPSFGLSVSRSQTFNQYFTRILTNVA 660
Query: 661 SQASTYRAMISTPKGLSITVNPNVLSFNGIGDRKSFKLTVRGTIRKDTVSAFLVWSDGVH 709
SQASTYRA IS+P+GL+ITVNP VLSFNGIGDRKSF LTV+GTI++ VSA LVW DGVH
Sbjct: 661 SQASTYRAAISSPQGLTITVNPTVLSFNGIGDRKSFTLTVKGTIKESVVSASLVWFDGVH 720
BLAST of Lsi04G009180 vs. NCBI nr
Match:
KAA0051615.1 (cucumisin-like [Cucumis melo var. makuwa])
HSP 1 Score: 1018.5 bits (2632), Expect = 2.9e-293
Identity = 522/725 (72.00%), Postives = 590/725 (81.38%), Query Frame = 0
Query: 7 MSSSLIFKLLFLNIFFSIQIASSLDSDNDGKKIYIVYMGRKLKDDRDSAHLHQSSFPFAP 66
MS SLI KL+F N+FF +ASSLDSD+ K+IYIVYMG+K KDD D A+LH SSFPFAP
Sbjct: 1 MSFSLILKLVFFNLFFRTLLASSLDSDD--KEIYIVYMGKKSKDDPDKANLHHSSFPFAP 60
Query: 67 ESVVYTYKRSFNGFAVKLTEEEAEKIAGMEGVVSVFPNKMKQLHTSRSWDFLGFPKNVPR 126
ESV+YTY RSFNGFAVKLT+EEA+KIA MEGVVSVFPN+M HT+RSWDF+GF +NVPR
Sbjct: 61 ESVLYTYNRSFNGFAVKLTKEEADKIASMEGVVSVFPNEMNTPHTTRSWDFMGFSQNVPR 120
Query: 127 VKQVESNIIVGVLDTGIWPESPSFDDKGFGPIPSKWKGTCQGLNFTCN------------ 186
VKQVESN++VGVLD+GIWPESPSF+D+GFGP PSKWKGTC +NFTCN
Sbjct: 121 VKQVESNVVVGVLDSGIWPESPSFNDQGFGPPPSKWKGTCSAINFTCNRKIIGARSYHIG 180
Query: 187 ---------SPRDINGHGTHTASTVAGSLVSHASLYGVGLGTARGGVPSARIAAYKICWN 246
PRD NGHGTHTASTVAG LVS ASLYG+GLGTARGGVPSARIA YK+CW
Sbjct: 181 RPLPLGDVEGPRDTNGHGTHTASTVAGGLVSQASLYGLGLGTARGGVPSARIAVYKVCW- 240
Query: 247 KIGCSQANILAAFDDAIADGVDIISISVGDGDLQPYFLDSVGIGSFHAIQNGILTSNSVG 306
+ CS A+ILAAFDDAIADGVDIIS+SVG + YF+DS+ IGSFHAI+ GILTSNS G
Sbjct: 241 RDACSDADILAAFDDAIADGVDIISLSVGRNVTRKYFIDSIAIGSFHAIEKGILTSNSAG 300
Query: 307 NSGPKLYSTTSVSPWLLSVAASTIDRKIVTQVQIGNKKTFQGVSINIFDMNGKYPLVAGH 366
N+GPKL +T S+SPWLLSVAASTIDRK VT+VQIGN+ +FQG SIN FD G+YPLV G
Sbjct: 301 NNGPKLKTTASLSPWLLSVAASTIDRKFVTKVQIGNQNSFQGFSINTFDNTGQYPLVTGR 360
Query: 367 DVLKAGFHNSISRSCRNNSVNPKLVKGKILICKELLSRDDFFSFGGAAGVLMV-SGTRDT 426
V GF ++IS C NNSV+ KLVKGKILIC+ F + GG AGVLM+ + D
Sbjct: 361 QVPNTGFDSNISSHCLNNSVDVKLVKGKILICEANFDAKHFVTLGGVAGVLMIDTELIDN 420
Query: 427 AFPQPLPSSTLDPNDADTIFHYIDSARSPTATIFKSTTLHNVSAPSLASFSSRGPNVLNK 486
A P+PS+ LD NDA + YI S SPTATIFKST N AP + SFSSRGPN + K
Sbjct: 421 ARSYPVPSAILDENDAIATYRYIYSNPSPTATIFKSTEQRNEPAPVVVSFSSRGPNNITK 480
Query: 487 DLVKPDLSAPGVEILAAWPPVAPVDGSHR-TLYNIVSGTSMACPHITGIAVYVKTFNPTW 546
+++KPDLS PGVEILAAWPPVA V G HR TLYNIVSGTSM+CPHITGIA YVKTFNPTW
Sbjct: 481 EIIKPDLSGPGVEILAAWPPVALVGGIHRNTLYNIVSGTSMSCPHITGIAAYVKTFNPTW 540
Query: 547 SPAAIKSALMTTASPMNSRFHPEAEFAYGSGHVNPLKAVRPGLVYDANESDYVKFLCGQG 606
SPAAIKSALMTTA PMN+ + +AEFAYG+GHVNPLKAVRPGLVYDANESDYVKFLCGQG
Sbjct: 541 SPAAIKSALMTTALPMNATLNSDAEFAYGAGHVNPLKAVRPGLVYDANESDYVKFLCGQG 600
Query: 607 YTTNMVRTITNDNSACTSENIGRVWDLNSPSFGLSVSHSKTFNQYFTRTLTSVASQASTY 666
YTTNMVR+ITNDNSACT+ NIGRVWDLN PSFGLSVS S+TFNQYFTR LT+VASQASTY
Sbjct: 601 YTTNMVRSITNDNSACTASNIGRVWDLNYPSFGLSVSRSQTFNQYFTRILTNVASQASTY 660
Query: 667 RAMISTPKGLSITVNPNVLSFNGIGDRKSFKLTVRGTIRKDTVSAFLVWSDGVHTVRSPI 709
RA IS+P+GL+ITVNP VLSFNGIGDRKSF LTV+GTI++ VSA LVW DGVH+VRSPI
Sbjct: 661 RAAISSPQGLTITVNPTVLSFNGIGDRKSFTLTVKGTIKESVVSASLVWFDGVHSVRSPI 720
BLAST of Lsi04G009180 vs. NCBI nr
Match:
XP_008461721.1 (PREDICTED: LOW QUALITY PROTEIN: cucumisin [Cucumis melo])
HSP 1 Score: 1007.3 bits (2603), Expect = 6.6e-290
Identity = 525/733 (71.62%), Postives = 586/733 (79.95%), Query Frame = 0
Query: 7 MSSSLIFKLLFLNIFFSIQIASSLDSDNDGKKIYIVYMGRKLKDDRDSAHLHQSSF---- 66
MSSSLIFKL F ++FFS ++AS LDSD+DGK IYIVYMGRKL +D DSAHLH +
Sbjct: 1 MSSSLIFKLFFFSLFFSNRLASRLDSDDDGKNIYIVYMGRKL-EDPDSAHLHHRAMLEQV 60
Query: 67 ---PFAPESVVYTYKRSFNGFAVKLTEEEAEKIAGMEGVVSVFPNKMKQLHTSRSWDFLG 126
FAPESV++TYKRSFNGFAVKLTEEEAEKIA MEGVVSVF N+M +LHT+RSWDFLG
Sbjct: 61 VGSTFAPESVLHTYKRSFNGFAVKLTEEEAEKIASMEGVVSVFLNEMNELHTTRSWDFLG 120
Query: 127 FPKNVPRVKQVESNIIVGVLDTGIWPESPSFDDKGFGPIPSKWKGTCQ-GLNFTC----- 186
FP VPR QVESNI+VGVLDTGIWPESPSFDD+GF P P KWKGTC+ NF C
Sbjct: 121 FPLTVPRRSQVESNIVVGVLDTGIWPESPSFDDEGFSPPPPKWKGTCETSNNFRCNRKII 180
Query: 187 ----------------NSPRDINGHGTHTASTVAGSLVSHASLYGVGLGTARGGVPSARI 246
N PRD NGHGTHTAST AG LVS A+LYG+GLGTARGGVP ARI
Sbjct: 181 GARSYHIGRPISPGDVNGPRDTNGHGTHTASTAAGGLVSQANLYGLGLGTARGGVPLARI 240
Query: 247 AAYKICWNKIGCSQANILAAFDDAIADGVDIISISVGDGDLQPYFLDSVGIGSFHAIQNG 306
AAYK+CWN GCS A+ILAA+DDAIADGVDIIS+SVG + + YF+D++ IGSFHA++ G
Sbjct: 241 AAYKVCWND-GCSDADILAAYDDAIADGVDIISLSVGGANPRHYFVDAIAIGSFHAVERG 300
Query: 307 ILTSNSVGNSGPKLYSTTSVSPWLLSVAASTIDRKIVTQVQIGNKKTFQGVSINIFDMNG 366
ILTSNS GN GP ++T S+SPWLLSVAAST+DRK VTQVQIGN ++FQGVSIN FD N
Sbjct: 301 ILTSNSAGNGGPNFFTTASLSPWLLSVAASTMDRKFVTQVQIGNGQSFQGVSINTFD-NQ 360
Query: 367 KYPLVAGHDVLKAGFHNSISRSCRNNSVNPKLVKGKILICKELLSRDDFF-SFGGAAGVL 426
YPLV+G D+ GF S SR C +NSV PKL+KGKI++C+ +FF S GAAGVL
Sbjct: 361 YYPLVSGRDIPNPGFDKSTSRFCTDNSVXPKLLKGKIVVCEASFGPHEFFKSLDGAAGVL 420
Query: 427 MVSGTRDTAFPQPLPSSTLDPNDADTIFHYIDSARSPTATIFKSTTLHNVSAPSLASFSS 486
M S TRD A PLPSS LDPND YI S RSP ATIFKSTT+ N SAP + SFSS
Sbjct: 421 MTSNTRDYADSYPLPSSVLDPNDLLATLRYIYSIRSPGATIFKSTTILNASAPVVVSFSS 480
Query: 487 RGPNVLNKDLVKPDLSAPGVEILAAWPPVAPVDGSHR-TLYNIVSGTSMACPHITGIAVY 546
RGPN KD++KPD+S PGVEILAAWP VAPV G R TL+NI+SGTSM+CPHITGIA Y
Sbjct: 481 RGPNRATKDVIKPDISGPGVEILAAWPSVAPVGGIRRNTLFNIISGTSMSCPHITGIATY 540
Query: 547 VKTFNPTWSPAAIKSALMTTASPMNSRFHPEAEFAYGSGHVNPLKAVRPGLVYDANESDY 606
VKT+NPTWSPAAIKSALMTTASPMN+RF+P+AEFAYGSGHVNPLKAVRPGLVYDANESDY
Sbjct: 541 VKTYNPTWSPAAIKSALMTTASPMNARFNPQAEFAYGSGHVNPLKAVRPGLVYDANESDY 600
Query: 607 VKFLCGQGYTTNMVRTITNDNSACTSENIGRVWDLNSPSFGLSVSHSKTFNQYFTRTLTS 666
VKFLCGQGY T VR IT D SACT N GRVWDLN PSFGLSVS SKTFNQYF RTLTS
Sbjct: 601 VKFLCGQGYNTEAVRRITGDYSACTPGNTGRVWDLNYPSFGLSVSPSKTFNQYFNRTLTS 660
Query: 667 VASQASTYRAMISTPKGLSITVNPNVLSFNGIGDRKSFKLTVRGTIRKDTVSAFLVWSDG 709
VA QASTYRAMIS P+GL+I+VNPNVLSFNG+GDRKSF LTVRG+I+ VSA LVWSDG
Sbjct: 661 VAPQASTYRAMISAPQGLTISVNPNVLSFNGLGDRKSFTLTVRGSIKGFVVSASLVWSDG 720
BLAST of Lsi04G009180 vs. TAIR 10
Match:
AT5G59120.1 (subtilase 4.13 )
HSP 1 Score: 593.6 bits (1529), Expect = 2.1e-169
Identity = 338/731 (46.24%), Postives = 458/731 (62.65%), Query Frame = 0
Query: 8 SSSLIFKLLFLNIFFSIQIASSLDSDNDGKKIYIVYMGRKLKDDRD----SAHLHQSSFP 67
SSSL+ LL L SS+ + D K++YIVYMG L D S H++
Sbjct: 7 SSSLLSCLLVL-------FLSSVSAVTDDKQVYIVYMG-SLSSRADYTPTSDHMNILQEV 66
Query: 68 FAPESV----VYTYKRSFNGFAVKLTEEEAEKIAGMEGVVSVFPNKMKQLHTSRSWDFLG 127
S+ V +YKRSFNGFA +LTE E E++A M GVVSVFPNK QL T+ SWDF+G
Sbjct: 67 TGESSIEGRLVRSYKRSFNGFAARLTESERERVAKMVGVVSVFPNKKLQLQTTTSWDFMG 126
Query: 128 FPKNV--PRVKQVESNIIVGVLDTGIWPESPSFDDKGFGPIPSKWKGTCQ-GLNFTCNS- 187
+ + R VES+ I+GV+D+GI PES SF DKGFGP P KWKG C G NFTCN+
Sbjct: 127 LKEGIKTKRNPTVESDTIIGVIDSGITPESQSFSDKGFGPPPQKWKGVCSGGKNFTCNNK 186
Query: 188 -----------PRDINGHGTHTASTVAGSLVSHASLYGVGLGTARGGVPSARIAAYKICW 247
RD++GHGTHTAST AG+ V AS +G+G GT RGGVP++R+AAYK+C
Sbjct: 187 LIGARDYTSEGTRDMDGHGTHTASTAAGNAVVDASFFGIGNGTVRGGVPASRVAAYKVC- 246
Query: 248 NKIGCSQANILAAFDDAIADGVDIISISVGDGDLQPYFLDSVGIGSFHAIQNGILTSNSV 307
GCS +L+AFDDAIADGVD+I+IS+GD + D + IG+FHA+ G+LT NS
Sbjct: 247 TPTGCSSEALLSAFDDAIADGVDLITISIGDKTASMFQNDPIAIGAFHAMAKGVLTVNSA 306
Query: 308 GNSGPKLYSTTSVSPWLLSVAASTIDRKIVTQVQIGNKKTFQGVSINIFDMNGK-YPLVA 367
GNSGPK S + V+PW+L+VAAST +R VT+V +GN KT G S+N ++M GK YPLV
Sbjct: 307 GNSGPKPISVSGVAPWILTVAASTTNRGFVTKVVLGNGKTLVGKSVNAYEMKGKDYPLVY 366
Query: 368 GHDVLKAGFHNSISRSCRNNSVNPKLVKGKILICKELLSRDDFFSFGGAAGVLMVSGTRD 427
G + + C + V+ VKGKIL+C S GA G++ + D
Sbjct: 367 GKSAASSACDAESAGLCELSCVDKSRVKGKILVCGGPGGLKIVESV-GAVGLIYRTPKPD 426
Query: 428 TAFPQPLPSSTLDPNDADTIFHYIDSARSPTATIFKSTTLHNVSAPSLASFSSRGPNVLN 487
AF PLP++ L D +++ Y++S SP A + K+ + N ++P +ASFSSRGPN +
Sbjct: 427 VAFIHPLPAAGLLTEDFESLVSYLESTDSPQAIVLKTEAIFNRTSPVIASFSSRGPNTIA 486
Query: 488 KDLVKPDLSAPGVEILAAWPPVA--PVDGSHRTLYNIVSGTSMACPHITGIAVYVKTFNP 547
D++KPD++APGVEILAA+ P D + Y+++SGTSM+CPH+ G+A YVKTFNP
Sbjct: 487 VDILKPDITAPGVEILAAYSPAGEPSQDDTRHVKYSVLSGTSMSCPHVAGVAAYVKTFNP 546
Query: 548 TWSPAAIKSALMTTASPMNSRFH--PEAEFAYGSGHVNPLKAVRPGLVYDANESDYVKFL 607
WSP+ I+SA+MTTA P+N+ EFAYGSGHV+P+ A PGLVY+ ++SD++ FL
Sbjct: 547 KWSPSMIQSAIMTTAWPVNATGTGIASTEFAYGSGHVDPIAASNPGLVYELDKSDHIAFL 606
Query: 608 CGQGYTTNMVRTITNDNSACTSENIGRVWDLNSPSFGLSVSHS-KTFNQYFTRTLTSVAS 667
CG YT+ +++ I+ + C+ +LN PS +S S TF F RTLT+V +
Sbjct: 607 CGMNYTSQVLKVISGETVTCSEAKKILPRNLNYPSMSAKLSGSGTTFTVTFNRTLTNVGT 666
Query: 668 QASTYRAMISTPKG--LSITVNPNVLSFNGIGDRKSFKLTVRGTIRKDTV--SAFLVWSD 706
STY + + G L + + P+VLSF + +++SF +TV G+ V SA L+WSD
Sbjct: 667 PNSTYTSKVVAGHGSKLDVKITPSVLSFKTVNEKQSFTVTVTGSNLDSEVPSSANLIWSD 726
BLAST of Lsi04G009180 vs. TAIR 10
Match:
AT5G59190.1 (subtilase family protein )
HSP 1 Score: 589.3 bits (1518), Expect = 4.0e-168
Identity = 317/669 (47.38%), Postives = 438/669 (65.47%), Query Frame = 0
Query: 65 APESVVYTYKRSFNGFAVKLTEEEAEKIAGMEGVVSVFPNKMKQLHTSRSWDFLGFPKNV 124
A +V +YKRSFNGFA L++ E++K+ M+ VVSVFP+K +L T+RSWDF+GF +
Sbjct: 28 ASHLLVRSYKRSFNGFAANLSQAESQKLQNMKEVVSVFPSKSHELTTTRSWDFVGFGEKA 87
Query: 125 PRVKQVESNIIVGVLDTGIWPESPSFDDKGFGPIPSKWKGTCQ-GLNFTCN--------- 184
R ES++IVGV+D+GIWPES SFDD+GFGP P KWKG+C+ GL F CN
Sbjct: 88 RRESVKESDVIVGVIDSGIWPESESFDDEGFGPPPKKWKGSCKGGLKFACNNKLIGARFY 147
Query: 185 -----SPRDINGHGTHTASTVAGSLVSHASLYGVGLGTARGGVPSARIAAYKICWNKIGC 244
S RD GHGTHTAST AG+ V AS YG+ GTARGGVPSARIAAYK+C+N+ C
Sbjct: 148 NKFADSARDEEGHGTHTASTAAGNAVQAASFYGLAQGTARGGVPSARIAAYKVCFNR--C 207
Query: 245 SQANILAAFDDAIADGVDIISISVGDGDLQPYFLDSVGIGSFHAIQNGILTSNSVGNSGP 304
+ +ILAAFDDAIADGVD+ISIS+ + SV IGSFHA+ GI+T+ S GN+GP
Sbjct: 208 NDVDILAAFDDAIADGVDVISISISADYVSNLLNASVAIGSFHAMMRGIITAGSAGNNGP 267
Query: 305 KLYSTTSVSPWLLSVAASTIDRKIVTQVQIGNKKTFQGVSINIFDMNG-KYPLVAGHDVL 364
S +VSPW+++VAAS DR+ + +V +GN K G+S+N F++NG K+P+V G +V
Sbjct: 268 DQGSVANVSPWMITVAASGTDRQFIDRVVLGNGKALTGISVNTFNLNGTKFPIVYGQNVS 327
Query: 365 KAGFHNSISRSCRNNSVNPKLVKGKILICKELLSRDDFFSFGGAAGVLMVSGTRDTAFPQ 424
+ + + C + V+ +LVKGKI++C + L + + G ++ + D+AF
Sbjct: 328 R-NCSQAQAGYCSSGCVDSELVKGKIVLCDDFLGYREAYLAGAIGVIVQNTLLPDSAFVV 387
Query: 425 PLPSSTLDPNDADTIFHYIDSARSPTATIFKSTTLHNVSAPSLASFSSRGPNVLNKDLVK 484
P P+S+L D +I YI+SA P A I ++ + + AP + SFSSRGP+ + ++L+K
Sbjct: 388 PFPASSLGFEDYKSIKSYIESAEPPQAEILRTEEIVDREAPYVPSFSSRGPSFVIQNLLK 447
Query: 485 PDLSAPGVEILAAWPPVAP------VDGSHRTLYNIVSGTSMACPHITGIAVYVKTFNPT 544
PD+SAPG+EILAA+ PVA + Y+++SGTSMACPH+ G+A YVK+F+P
Sbjct: 448 PDVSAPGLEILAAFSPVASPSSFLNPEDKRSVRYSVMSGTSMACPHVAGVAAYVKSFHPD 507
Query: 545 WSPAAIKSALMTTASPMNSRFHPEAEFAYGSGHVNPLKAVRPGLVYDANESDYVKFLCGQ 604
WSP+AIKSA+MTTA+PMN + +PE EFAYGSG +NP KA PGLVY+ DY+K LC +
Sbjct: 508 WSPSAIKSAIMTTATPMNLKKNPEQEFAYGSGQINPTKASDPGLVYEVETEDYLKMLCAE 567
Query: 605 GYTTNMVRTITNDNSACTSENIGRVWDLNSPSFGLSVSHSKTFNQYFTRTLTSVASQAST 664
G+ + + T + N C+ V DLN P+ VS FN F RT+T+V ST
Sbjct: 568 GFDSTTLTTTSGQNVTCSERT--EVKDLNYPTMTTFVSSLDPFNVTFKRTVTNVGFPNST 627
Query: 665 YRA-MISTPKGLSITVNPNVLSFNGIGDRKSFKLTVRGTIRKD--TVSAFLVWSDGVHTV 709
Y+A ++ L I++ P +L F + ++KSF +T+ G KD VS+ +VWSDG H+V
Sbjct: 628 YKASVVPLQPELQISIEPEILRFGFLEEKKSFVVTISGKELKDGSFVSSSVVWSDGSHSV 687
BLAST of Lsi04G009180 vs. TAIR 10
Match:
AT5G59100.1 (Subtilisin-like serine endopeptidase family protein )
HSP 1 Score: 585.5 bits (1508), Expect = 5.8e-167
Identity = 331/746 (44.37%), Postives = 472/746 (63.27%), Query Frame = 0
Query: 7 MSSSLIFKLLFLNIFFSIQIASSLDSDNDG-KKIYIVYMGR-KLKDDRDSAHLHQSSF-- 66
M+ F LF ++ + S D D+ G +++YIVY+G +++ H S
Sbjct: 1 MAKGTTFIFLFSSLLVLSLSSVSADKDDHGDQQVYIVYLGSLPSREEYTPMSDHMSILQE 60
Query: 67 ----PFAPESVVYTYKRSFNGFAVKLTEEEAEKIAGMEGVVSVFPNKMKQLHTSRSWDFL 126
+V +YK+SFNGFA +LTE E +++AGME VVSVFP++ +L T+ SW+F+
Sbjct: 61 ITGESLIENRLVRSYKKSFNGFAARLTESERKRLAGMERVVSVFPSRKLKLQTTSSWNFM 120
Query: 127 GFPKNV--PRVKQVESNIIVGVLDTGIWPESPSFDDKGFGPIPSKWKGTCQ-GLNFTCN- 186
G + + R + +ES+ I+GV+D+GI+PES SF D+GFGP P KWKGTC G NFTCN
Sbjct: 121 GLKEGIKTKRTRSIESDTIIGVIDSGIYPESDSFSDQGFGPPPKKWKGTCAGGKNFTCNN 180
Query: 187 ----------------SPRDINGHGTHTASTVAGSLVSHASLYGVGLGTARGGVPSARIA 246
+ RD +GHGTHTAS AG+ V++++ YG+G GTARGGVP+ARIA
Sbjct: 181 KVIGARDYTAKSKANQTARDYSGHGTHTASIAAGNAVANSNFYGLGNGTARGGVPAARIA 240
Query: 247 AYKICWNKIGCSQANILAAFDDAIADGVDIISISVGDGDLQPYFLDSVGIGSFHAIQNGI 306
YK+C N+ GC +++AFDDAIADGVD+ISIS+ ++ P+ D + IG+FHA+ G+
Sbjct: 241 VYKVCDNE-GCDGEAMMSAFDDAIADGVDVISISIVLDNIPPFEEDPIAIGAFHAMAVGV 300
Query: 307 LTSNSVGNSGPKLYSTTSVSPWLLSVAASTIDRKIVTQVQIGNKKTFQGVSINIFDMNG- 366
LT N+ GN+GPK+ + TS +PW+ SVAAS +R + +V +G+ K G S+N +DMNG
Sbjct: 301 LTVNAAGNNGPKISTVTSTAPWVFSVAASVTNRAFMAKVVLGDGKILIGRSVNTYDMNGT 360
Query: 367 KYPLVAGHDVLKAGFHNSISRSCRNNSVNPKLVKGKILIC---KELLSRDDFFSFGGAAG 426
YPLV G + +R C ++ KLVKGKI++C K L+ GA G
Sbjct: 361 NYPLVYGKSAALSTCSVDKARLCEPKCLDGKLVKGKIVLCDSTKGLIEAQKL----GAVG 420
Query: 427 VLMVSGTRDTAFPQPLPSSTLDPNDADTIFHYIDSARSPTATIFKSTTLHNVSAPSLASF 486
++ + D AF + P S L +D ++ Y++S ++P AT+ KS + N AP +ASF
Sbjct: 421 SIVKNPEPDRAFIRSFPVSFLSNDDYKSLVSYMNSTKNPKATVLKSEEISNQRAPLVASF 480
Query: 487 SSRGPNVLNKDLVKPDLSAPGVEILAAW-PPVAPVDG---SHRTLYNIVSGTSMACPHIT 546
SSRGP+ + D++KPD++APGVEILAA+ P +P + + R Y+++SGTSMACPH+
Sbjct: 481 SSRGPSSIVSDILKPDITAPGVEILAAYSPDSSPTESEFDTRRVKYSVLSGTSMACPHVA 540
Query: 547 GIAVYVKTFNPTWSPAAIKSALMTTASPMNS--RFHPEAEFAYGSGHVNPLKAVRPGLVY 606
G+A YVKTF+P WSP+ I+SA+MTTA PMN+ EFAYGSGHV+P+ A+ PGLVY
Sbjct: 541 GVAAYVKTFHPQWSPSMIQSAIMTTAWPMNASGSGFVSTEFAYGSGHVDPIDAINPGLVY 600
Query: 607 DANESDYVKFLCGQGYTTNMVRTITNDNSACTSENIGRVW--DLNSPSFGLSVSHSKTFN 666
+ ++D++ FLCG YT++ +R I+ DNS CT E I + +LN P+ VS +K FN
Sbjct: 601 ELTKADHINFLCGLNYTSDHLRIISGDNSTCTKE-ISKTLPRNLNYPTMSAKVSGTKPFN 660
Query: 667 QYFTRTLTSVASQASTYRAMISTPKG--LSITVNPNVLSFNGIGDRKSFKLTVRGTI--R 709
F RT+T+V Q STY A + G LSI V+P VLS + +++SF +TV
Sbjct: 661 ITFQRTVTNVGMQKSTYNAKVVKFPGSKLSIKVSPRVLSMKSMNEKQSFMVTVSSDSIGT 720
BLAST of Lsi04G009180 vs. TAIR 10
Match:
AT5G59090.1 (subtilase 4.12 )
HSP 1 Score: 584.7 bits (1506), Expect = 9.8e-167
Identity = 339/736 (46.06%), Postives = 462/736 (62.77%), Query Frame = 0
Query: 3 LSTTMSSSLIFKLLFLNIFFSIQIASSLDSDNDGKKIYIVYMGRKLKDDRD----SAH-- 62
++ +S+ ++ L + + S +++ +D D ++YIVYMG L D S H
Sbjct: 1 MANLAASTCLYSWLLVLLLSS--VSAIIDEDT---QVYIVYMG-SLSSRADYIPTSDHMS 60
Query: 63 -LHQSSFPFAPES-VVYTYKRSFNGFAVKLTEEEAEKIAGMEGVVSVFPNKMKQLHTSRS 122
L Q + + E +V +YKRSFNGFA +LTE E IA +EGVVSVFPNK+ QLHT+ S
Sbjct: 61 ILQQVTGESSIEGRLVRSYKRSFNGFAARLTESERTLIAEIEGVVSVFPNKILQLHTTTS 120
Query: 123 WDFLGFP--KNVPRVKQVESNIIVGVLDTGIWPESPSFDDKGFGPIPSKWKGTCQ-GLNF 182
WDF+G KN R +ES+ I+GV+DTGIWPES SF DKGFGP P KWKG C G NF
Sbjct: 121 WDFMGVKEGKNTKRNLAIESDTIIGVIDTGIWPESKSFSDKGFGPPPKKWKGVCSGGKNF 180
Query: 183 TCNS------------PRDINGHGTHTASTVAGSLVSHASLYGVGLGTARGGVPSARIAA 242
TCN+ RD +GHGTHTAST AG+ V S +G+G GT RGGVP++RIAA
Sbjct: 181 TCNNKLIGARDYTSEGTRDTSGHGTHTASTAAGNAVKDTSFFGIGNGTVRGGVPASRIAA 240
Query: 243 YKICWNKIGCSQANILAAFDDAIADGVDIISISVGDGDLQPYFLDSVGIGSFHAIQNGIL 302
YK+C + GCS +L++FDDAIADGVD+I+IS+G + D + IG+FHA+ GIL
Sbjct: 241 YKVCTDS-GCSSEALLSSFDDAIADGVDLITISIGFQFPSIFEDDPIAIGAFHAMAKGIL 300
Query: 303 TSNSVGNSGPKLYSTTSVSPWLLSVAASTIDRKIVTQVQIGNKKTFQGVSINIFDMNG-K 362
T +S GNSGPK + + V+PW+ +VAAST +R +T+V +GN KT G S+N FDM G K
Sbjct: 301 TVSSAGNSGPKPTTVSHVAPWIFTVAASTTNRGFITKVVLGNGKTLAGRSVNAFDMKGKK 360
Query: 363 YPLVAGHDVLKAGFHNSISRSCRNNSVNPKLVKGKILICKELLSRDDFFSFGGAAGVLMV 422
YPLV G + + C +N VKGKIL+C S GA ++
Sbjct: 361 YPLVYGKSAASSACDAKTAALCAPACLNKSRVKGKILVCGG-PSGYKIAKSVGAIAIIDK 420
Query: 423 SGTRDTAFPQPLPSSTLDPNDADTIFHYIDSARSPTATIFKSTTLHNVSAPSLASFSSRG 482
S D AF LP+S L D ++ YI+S SP A + K+ T+ N ++P +ASFSSRG
Sbjct: 421 SPRPDVAFTHHLPASGLKAKDFKSLVSYIESQDSPQAAVLKTETIFNRTSPVIASFSSRG 480
Query: 483 PNVLNKDLVKPDLSAPGVEILAAWPP--VAPVDGSHRTLYNIVSGTSMACPHITGIAVYV 542
PN + D++KPD++APGVEILAA+ P D + R Y++ SGTSMACPH+ G+A YV
Sbjct: 481 PNTIAVDILKPDITAPGVEILAAFSPNGEPSEDDTRRVKYSVFSGTSMACPHVAGVAAYV 540
Query: 543 KTFNPTWSPAAIKSALMTTASPMNS--RFHPEAEFAYGSGHVNPLKAVRPGLVYDANESD 602
KTF P WSP+ I+SA+MTTA P+ + R EFAYG+GHV+P+ A+ PGLVY+ +++D
Sbjct: 541 KTFYPRWSPSMIQSAIMTTAWPVKAKGRGIASTEFAYGAGHVDPMAALNPGLVYELDKAD 600
Query: 603 YVKFLCGQGYTTNMVRTITNDNSACTSENIGRVWDLNSPSFGLSVSHS-KTFNQYFTRTL 662
++ FLCG YT+ ++ I+ D C+ +N +LN PS +S + TF+ F RTL
Sbjct: 601 HIAFLCGMNYTSKTLKIISGDTVKCSKKNKILPRNLNYPSMSAKLSGTDSTFSVTFNRTL 660
Query: 663 TSVASQASTYRAMISTPKG--LSITVNPNVLSFNGIGDRKSFKLTVRGTIRKDTV--SAF 706
T+V + STY++ + G LSI V P+VL F + +++SF +TV G+ V SA
Sbjct: 661 TNVGTPNSTYKSKVVAGHGSKLSIKVTPSVLYFKTVNEKQSFSVTVTGSDVDSEVPSSAN 720
BLAST of Lsi04G009180 vs. TAIR 10
Match:
AT5G59090.2 (subtilase 4.12 )
HSP 1 Score: 582.4 bits (1500), Expect = 4.9e-166
Identity = 338/734 (46.05%), Postives = 459/734 (62.53%), Query Frame = 0
Query: 3 LSTTMSSSLIFKLLFLNIFFSIQIASSLDSDNDGKKIYIVYMGRKLKDDRD----SAH-- 62
++ +S+ ++ L + + S +++ +D D ++YIVYMG L D S H
Sbjct: 1 MANLAASTCLYSWLLVLLLSS--VSAIIDEDT---QVYIVYMG-SLSSRADYIPTSDHMS 60
Query: 63 -LHQSSFPFAPES-VVYTYKRSFNGFAVKLTEEEAEKIAGMEGVVSVFPNKMKQLHTSRS 122
L Q + + E +V +YKRSFNGFA +LTE E IA +EGVVSVFPNK+ QLHT+ S
Sbjct: 61 ILQQVTGESSIEGRLVRSYKRSFNGFAARLTESERTLIAEIEGVVSVFPNKILQLHTTTS 120
Query: 123 WDFLGFP--KNVPRVKQVESNIIVGVLDTGIWPESPSFDDKGFGPIPSKWKGTCQ-GLNF 182
WDF+G KN R +ES+ I+GV+DTGIWPES SF DKGFGP P KWKG C G NF
Sbjct: 121 WDFMGVKEGKNTKRNLAIESDTIIGVIDTGIWPESKSFSDKGFGPPPKKWKGVCSGGKNF 180
Query: 183 TCNS------------PRDINGHGTHTASTVAGSLVSHASLYGVGLGTARGGVPSARIAA 242
TCN+ RD +GHGTHTAST AG+ V S +G+G GT RGGVP++RIAA
Sbjct: 181 TCNNKLIGARDYTSEGTRDTSGHGTHTASTAAGNAVKDTSFFGIGNGTVRGGVPASRIAA 240
Query: 243 YKICWNKIGCSQANILAAFDDAIADGVDIISISVGDGDLQPYFLDSVGIGSFHAIQNGIL 302
YK+C + GCS +L++FDDAIADGVD+I+IS+G + D + IG+FHA+ GIL
Sbjct: 241 YKVCTDS-GCSSEALLSSFDDAIADGVDLITISIGFQFPSIFEDDPIAIGAFHAMAKGIL 300
Query: 303 TSNSVGNSGPKLYSTTSVSPWLLSVAASTIDRKIVTQVQIGNKKTFQGVSINIFDMNG-K 362
T +S GNSGPK + + V+PW+ +VAAST +R +T+V +GN KT G S+N FDM G K
Sbjct: 301 TVSSAGNSGPKPTTVSHVAPWIFTVAASTTNRGFITKVVLGNGKTLAGRSVNAFDMKGKK 360
Query: 363 YPLVAGHDVLKAGFHNSISRSCRNNSVNPKLVKGKILICKELLSRDDFFSFGGAAGVLMV 422
YPLV G + + C +N VKGKIL+C S GA ++
Sbjct: 361 YPLVYGKSAASSACDAKTAALCAPACLNKSRVKGKILVCGG-PSGYKIAKSVGAIAIIDK 420
Query: 423 SGTRDTAFPQPLPSSTLDPNDADTIFHYIDSARSPTATIFKSTTLHNVSAPSLASFSSRG 482
S D AF LP+S L D ++ YI+S SP A + K+ T+ N ++P +ASFSSRG
Sbjct: 421 SPRPDVAFTHHLPASGLKAKDFKSLVSYIESQDSPQAAVLKTETIFNRTSPVIASFSSRG 480
Query: 483 PNVLNKDLVKPDLSAPGVEILAAWPP--VAPVDGSHRTLYNIVSGTSMACPHITGIAVYV 542
PN + D++KPD++APGVEILAA+ P D + R Y++ SGTSMACPH+ G+A YV
Sbjct: 481 PNTIAVDILKPDITAPGVEILAAFSPNGEPSEDDTRRVKYSVFSGTSMACPHVAGVAAYV 540
Query: 543 KTFNPTWSPAAIKSALMTTASPMNSRFHPEAEFAYGSGHVNPLKAVRPGLVYDANESDYV 602
KTF P WSP+ I+SA+MTTA R EFAYG+GHV+P+ A+ PGLVY+ +++D++
Sbjct: 541 KTFYPRWSPSMIQSAIMTTA---KGRGIASTEFAYGAGHVDPMAALNPGLVYELDKADHI 600
Query: 603 KFLCGQGYTTNMVRTITNDNSACTSENIGRVWDLNSPSFGLSVSHS-KTFNQYFTRTLTS 662
FLCG YT+ ++ I+ D C+ +N +LN PS +S + TF+ F RTLT+
Sbjct: 601 AFLCGMNYTSKTLKIISGDTVKCSKKNKILPRNLNYPSMSAKLSGTDSTFSVTFNRTLTN 660
Query: 663 VASQASTYRAMISTPKG--LSITVNPNVLSFNGIGDRKSFKLTVRGTIRKDTV--SAFLV 706
V + STY++ + G LSI V P+VL F + +++SF +TV G+ V SA L+
Sbjct: 661 VGTPNSTYKSKVVAGHGSKLSIKVTPSVLYFKTVNEKQSFSVTVTGSDVDSEVPSSANLI 720
The following BLAST results are available for this feature:
| Match Name | E-value | Identity | Description | |
|---|
| Q39547 | 9.6e-292 | 71.35 | Cucumisin OS=Cucumis melo OX=3656 PE=1 SV=1 | [more] |
| Q9FIF8 | 1.7e-168 | 46.13 | Subtilisin-like protease SBT4.3 OS=Arabidopsis thaliana OX=3702 GN=SBT4.3 PE=3 S... | [more] |
| Q9FIG2 | 3.0e-168 | 46.24 | Subtilisin-like protease SBT4.13 OS=Arabidopsis thaliana OX=3702 GN=SBT4.13 PE=2... | [more] |
| Q9FGU3 | 8.1e-166 | 44.37 | Subtilisin-like protease SBT4.4 OS=Arabidopsis thaliana OX=3702 GN=SBT4.4 PE=2 S... | [more] |
| Q8L7D2 | 1.4e-165 | 46.06 | Subtilisin-like protease SBT4.12 OS=Arabidopsis thaliana OX=3702 GN=SBT4.12 PE=2... | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| A0A1S3CFD6 | 2.1e-294 | 71.82 | cucumisin-like OS=Cucumis melo OX=3656 GN=LOC103500254 PE=3 SV=1 | [more] |
| A0A5A7UD73 | 1.4e-293 | 72.00 | Cucumisin-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold174G00164... | [more] |
| A0A1S3CF86 | 3.2e-290 | 71.62 | LOW QUALITY PROTEIN: cucumisin OS=Cucumis melo OX=3656 GN=LOC103500253 PE=3 SV=1 | [more] |
| A0A1S4E3D9 | 1.7e-283 | 69.63 | LOW QUALITY PROTEIN: cucumisin-like OS=Cucumis melo OX=3656 GN=LOC103500257 PE=3... | [more] |
| A0A5D3E4N6 | 3.5e-281 | 70.07 | Cucumisin-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold267G00100... | [more] |
