Lsi04G008990 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi04G008990
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Descriptionribulose bisphosphate carboxylase/oxygenase activase, chloroplastic
Locationchr04: 9036343 .. 9039432 (-)
RNA-Seq ExpressionLsi04G008990
SyntenyLsi04G008990
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATTTACAGCCCTTGTCCTTCTGCCACGTGTCCTCTCAGCTAATCTCAACTGTGTGGTTGTTGAGAATTTCTGAAACTCATGGTTAGGATATCATTTTTCAATGGACTCAAAAACTCCATCCCTTTACTTCTATAAAGTCCCTCCTCAGTCCTCACTGCTGTTTATCCTTCTCCAAATCTAGCTTCCTCTGCCAATACTTTCACACCCAAAGTCCCGATGGCCGCCTCCGCTGCCTCCGTCGGAGTCGTTAACCATGCACCGGTGAGTATGAGTTCTTCTATTAAGTTAATTAATAGTAATAACAGTTCTGATTAGATGTCTTGGCTCTAATACCATGTTAAAATTTGATTACTGGAAATTTCAGTTGAGCTTGAATGGCTCTGGATCGGCAACTTCAGTTCCGAGCTCAGTGTTCTTCGGAAACAGTTTGAAGAAAGTGGTGAACTCAAGAATTGTGAACCCGAAGGCATCATCGGGCAGCTTTAAAGTGGTGGCAGCGGATTCCGTCCCGGACGAGAATCTCGATAAGCAGACATCAAAAGACAGATGGAGAGGGCTCGGATATGACCAGTCAGATGACCAACAGGACATCACAAGAGGAAAGGGAATGGTGGACACTCTGTTTCAAGCCCCCATGCAAAGTGGAACTCATTATGCTGTTATGAGCTCTTATGAGTATCTTAGTACAGGACTTAAAACGTAAGACACACACAAACAAACAGAAAAAAAAAAAAAAAAAACAGAACAGTTCAAATTTGTTGTTGTTTTTTTGAGCTGATATCTTGTTTTTGTATGTGTTGTAGGTATGAGGGGTTGGACAATTTATTGGATGGTTACTACATTGCTCCTGCTTTCATGGACAAGTTGGTTATTCACATCACCAAGAACTACATGACCCTCCCAAACATCAAGGTTTCTAACCCTTTAAATTCATCTATACTGAGTCTCTGACGCTTTCGAGTCAAAACAGTTCTTTTTATAAACATGAGACTATTAGATAACATGGGATTTCATCTCAAAACTAATTGGCAATGAAAGCTTCAAGATGGTGCTTCTTTTAAGTTCACCATTCTTCGATCGGTTTCATCTCAAAACCAATTGGTAATAGGAGGAGTAGCTCACATATCTTGTAAACATTGTGAAGTCCGATCTCTTAATGTTTCCAACGTGAGATCTTCAACATGCCCCCTTAAGACGGTGTGTGTTTTGGGTTCAATATTCTTGAATCGAATCTCAATTTTTTTGTTTTTAGAACAAATACACGTTTGGGGTTCATGAGCTCTGATATCATATTAGATTATTTGGAGTTCCATCTTAAAAATAAAAAATAAAAAAAGTAAGAACTTGAGGTCCCCTCGATAAAAATCATTATATTTATATCATAAGGAAATTCAATATATAGAAGTTTTTGTGTCAACTGGTCATACTGAAGTGTTTATTTAGTGTATTCTTTCAAAATTATATATATATATATAATTTTCTTTTTATTTCATACTATGAAGATATATGATATATAACCCTTTTAAAAGTGATTTCAAAATTGAATAAGAAAATTCAAATTTTTCCTAAGAAGAAAAAGGTGAAAATTTCTGGCTTCAGGTTCCTCTGATTTTGGGTCTGTGGGGAGGCAAAGGACAAGGAAAATCCTTCCAATGTGAGCTTGTATTTGCCAAGATGGGAATCAAGTGAGTCCAAAATCCGATTTACAACTCACAATTTTCATAGTTTTTATAAAATTTGGTTTGCTCTTCTAACATTTTGGCCGTTTAATCTGTTTGTTACACAAAGCCCAATCATGATGAGTGCCGGAGAATTGGAAAGCGGCAACGCCGGAGAGCCAGCCAAGTTGATCCGACAACGGTACCGTGAGGCGGCTGACATAATCAAGAAGGGAAAAATGTCCTGCCTCTTCATCAATGATTTGGATGCCGGTGCTGGTCGTCTTGGTGGTACCACTCAATACACCGTCAACAACCAGATGGTTAACGCCACCCTCATGAACATCGCCGATAACCCGACCAATGTCCAGCTCCCTGGAATGTACAACAAGGAGGAGAACCCCCGAGTTCCAATCATCGTCACTGGTAACGACTTCTCTACTCTTTACGCTCCTCTCATCCGTGACGGTCGTATGGAGAAATTCTACTGGGCTCCCACTAGGGACGACCGTATCGGTGTCTGCAAAGGTATCTTCCGTACTGACAATGTTGCCGACGAGGATATCGTCAAGTTGGTCGACACCTTCCCAGGCCAATCCATCGGTCAGTTACTACTCATCACTCAAAAGTTTAAGCTAAAAATCACTTCTAGACGTGAAAATTAGTAAGAAAAAAGATTGAGAGAGAACATGACATTACATTTCAGGAGTTTGAATATAAAACCTCCTGCACACTGAAACAGGTAGACAAAGTACAGGCTGCTTAAACCCTTTTTTTCTGTTTTGTTGAATAGATTTCTTCGGTGCGCTAAGGGCGAGAGTGTACGATGACGAAGTAAGGAAGTGGATCATTGGAGTTGGAGTCCAGAGCATAGGGAAGAAGCTGGTGAACTCGAGGGAGCCACCACCGAAGTTTGAGCAGCCAACGATGACACTGGCAAAGCTGCTGGAGTATGGTAACATGCTGGTTCAAGAACAAGAGAATGTGAAGAGGGTGCAATTGGCTGACACATATTTGAATGAGGCTGCTCTTGGCAATGCCAATGAAGATGCCATTAAAAGAGGAACTTTCTAAATTAAGCTTGTTTGTTCTGATCTGTTGATCTGATTTGATGACACTCAAGTTGTTCTTTGTGGGGAAAGGAATGCTCATTATGGGATTTGCCTGATCTATATTCTTCAGGCCCTTTTGTTCTTTCTTCTTTTGTGGAACCAAAATCACATATCAAAATAAAAGTATATTTTTACTTTTGTTTCTCATTTTGTGACTTTTGTGTTTCAATGTGCTTTGTGAAAAAATTGTTGTATTTCTTCTTTTACTCATTCAAATGGTCAAAATGATCTGTTTTGCTTCTAAAATTTGTGTTCGGAATCACTCTTCATGTTTCCTACTTCTAGTTTTTGTATGAAAGAAATAGATTTAAATACTATTG

mRNA sequence

ATTTACAGCCCTTGTCCTTCTGCCACGTGTCCTCTCAGCTAATCTCAACTGTGTGGTTGTTGAGAATTTCTGAAACTCATGGTTAGGATATCATTTTTCAATGGACTCAAAAACTCCATCCCTTTACTTCTATAAAGTCCCTCCTCAGTCCTCACTGCTGTTTATCCTTCTCCAAATCTAGCTTCCTCTGCCAATACTTTCACACCCAAAGTCCCGATGGCCGCCTCCGCTGCCTCCGTCGGAGTCGTTAACCATGCACCGTTGAGCTTGAATGGCTCTGGATCGGCAACTTCAGTTCCGAGCTCAGTGTTCTTCGGAAACAGTTTGAAGAAAGTGGTGAACTCAAGAATTGTGAACCCGAAGGCATCATCGGGCAGCTTTAAAGTGGTGGCAGCGGATTCCGTCCCGGACGAGAATCTCGATAAGCAGACATCAAAAGACAGATGGAGAGGGCTCGGATATGACCAGTCAGATGACCAACAGGACATCACAAGAGGAAAGGGAATGGTGGACACTCTGTTTCAAGCCCCCATGCAAAGTGGAACTCATTATGCTGTTATGAGCTCTTATGAGTATCTTAGTACAGGACTTAAAACGTATGAGGGGTTGGACAATTTATTGGATGGTTACTACATTGCTCCTGCTTTCATGGACAAGTTGGTTATTCACATCACCAAGAACTACATGACCCTCCCAAACATCAAGGTTCCTCTGATTTTGGGTCTGTGGGGAGGCAAAGGACAAGGAAAATCCTTCCAATGTGAGCTTGTATTTGCCAAGATGGGAATCAACCCAATCATGATGAGTGCCGGAGAATTGGAAAGCGGCAACGCCGGAGAGCCAGCCAAGTTGATCCGACAACGGTACCGTGAGGCGGCTGACATAATCAAGAAGGGAAAAATGTCCTGCCTCTTCATCAATGATTTGGATGCCGGTGCTGGTCGTCTTGGTGGTACCACTCAATACACCGTCAACAACCAGATGGTTAACGCCACCCTCATGAACATCGCCGATAACCCGACCAATGTCCAGCTCCCTGGAATGTACAACAAGGAGGAGAACCCCCGAGTTCCAATCATCGTCACTGGTAACGACTTCTCTACTCTTTACGCTCCTCTCATCCGTGACGGTCGTATGGAGAAATTCTACTGGGCTCCCACTAGGGACGACCGTATCGGTGTCTGCAAAGGTATCTTCCGTACTGACAATGTTGCCGACGAGGATATCGTCAAGTTGGTCGACACCTTCCCAGGCCAATCCATCGATTTCTTCGGTGCGCTAAGGGCGAGAGTGTACGATGACGAAGTAAGGAAGTGGATCATTGGAGTTGGAGTCCAGAGCATAGGGAAGAAGCTGGTGAACTCGAGGGAGCCACCACCGAAGTTTGAGCAGCCAACGATGACACTGGCAAAGCTGCTGGAGTATGGTAACATGCTGGTTCAAGAACAAGAGAATGTGAAGAGGGTGCAATTGGCTGACACATATTTGAATGAGGCTGCTCTTGGCAATGCCAATGAAGATGCCATTAAAAGAGGAACTTTCTAAATTAAGCTTGTTTGTTCTGATCTGTTGATCTGATTTGATGACACTCAAGTTGTTCTTTGTGGGGAAAGGAATGCTCATTATGGGATTTGCCTGATCTATATTCTTCAGGCCCTTTTGTTCTTTCTTCTTTTGTGGAACCAAAATCACATATCAAAATAAAAGTATATTTTTACTTTTGTTTCTCATTTTGTGACTTTTGTGTTTCAATGTGCTTTGTGAAAAAATTGTTGTATTTCTTCTTTTACTCATTCAAATGGTCAAAATGATCTGTTTTGCTTCTAAAATTTGTGTTCGGAATCACTCTTCATGTTTCCTACTTCTAGTTTTTGTATGAAAGAAATAGATTTAAATACTATTG

Coding sequence (CDS)

ATGGCCGCCTCCGCTGCCTCCGTCGGAGTCGTTAACCATGCACCGTTGAGCTTGAATGGCTCTGGATCGGCAACTTCAGTTCCGAGCTCAGTGTTCTTCGGAAACAGTTTGAAGAAAGTGGTGAACTCAAGAATTGTGAACCCGAAGGCATCATCGGGCAGCTTTAAAGTGGTGGCAGCGGATTCCGTCCCGGACGAGAATCTCGATAAGCAGACATCAAAAGACAGATGGAGAGGGCTCGGATATGACCAGTCAGATGACCAACAGGACATCACAAGAGGAAAGGGAATGGTGGACACTCTGTTTCAAGCCCCCATGCAAAGTGGAACTCATTATGCTGTTATGAGCTCTTATGAGTATCTTAGTACAGGACTTAAAACGTATGAGGGGTTGGACAATTTATTGGATGGTTACTACATTGCTCCTGCTTTCATGGACAAGTTGGTTATTCACATCACCAAGAACTACATGACCCTCCCAAACATCAAGGTTCCTCTGATTTTGGGTCTGTGGGGAGGCAAAGGACAAGGAAAATCCTTCCAATGTGAGCTTGTATTTGCCAAGATGGGAATCAACCCAATCATGATGAGTGCCGGAGAATTGGAAAGCGGCAACGCCGGAGAGCCAGCCAAGTTGATCCGACAACGGTACCGTGAGGCGGCTGACATAATCAAGAAGGGAAAAATGTCCTGCCTCTTCATCAATGATTTGGATGCCGGTGCTGGTCGTCTTGGTGGTACCACTCAATACACCGTCAACAACCAGATGGTTAACGCCACCCTCATGAACATCGCCGATAACCCGACCAATGTCCAGCTCCCTGGAATGTACAACAAGGAGGAGAACCCCCGAGTTCCAATCATCGTCACTGGTAACGACTTCTCTACTCTTTACGCTCCTCTCATCCGTGACGGTCGTATGGAGAAATTCTACTGGGCTCCCACTAGGGACGACCGTATCGGTGTCTGCAAAGGTATCTTCCGTACTGACAATGTTGCCGACGAGGATATCGTCAAGTTGGTCGACACCTTCCCAGGCCAATCCATCGATTTCTTCGGTGCGCTAAGGGCGAGAGTGTACGATGACGAAGTAAGGAAGTGGATCATTGGAGTTGGAGTCCAGAGCATAGGGAAGAAGCTGGTGAACTCGAGGGAGCCACCACCGAAGTTTGAGCAGCCAACGATGACACTGGCAAAGCTGCTGGAGTATGGTAACATGCTGGTTCAAGAACAAGAGAATGTGAAGAGGGTGCAATTGGCTGACACATATTTGAATGAGGCTGCTCTTGGCAATGCCAATGAAGATGCCATTAAAAGAGGAACTTTCTAA

Protein sequence

MAASAASVGVVNHAPLSLNGSGSATSVPSSVFFGNSLKKVVNSRIVNPKASSGSFKVVAADSVPDENLDKQTSKDRWRGLGYDQSDDQQDITRGKGMVDTLFQAPMQSGTHYAVMSSYEYLSTGLKTYEGLDNLLDGYYIAPAFMDKLVIHITKNYMTLPNIKVPLILGLWGGKGQGKSFQCELVFAKMGINPIMMSAGELESGNAGEPAKLIRQRYREAADIIKKGKMSCLFINDLDAGAGRLGGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKEENPRVPIIVTGNDFSTLYAPLIRDGRMEKFYWAPTRDDRIGVCKGIFRTDNVADEDIVKLVDTFPGQSIDFFGALRARVYDDEVRKWIIGVGVQSIGKKLVNSREPPPKFEQPTMTLAKLLEYGNMLVQEQENVKRVQLADTYLNEAALGNANEDAIKRGTF
Homology
BLAST of Lsi04G008990 vs. ExPASy Swiss-Prot
Match: O98997 (Ribulose bisphosphate carboxylase/oxygenase activase, chloroplastic OS=Vigna radiata var. radiata OX=3916 GN=RCA PE=2 SV=2)

HSP 1 Score: 734.9 bits (1896), Expect = 5.2e-211
Identity = 366/442 (82.81%), Postives = 398/442 (90.05%), Query Frame = 0

Query: 1   MAASAASVGVVNHAPLSLNGSGSATSVPSSVFFGNSLKKVVNSRIVNPKASSGSFKVVAA 60
           MAAS ++VG VN A L+LNGSG+  S P+S FFG SLKK V SR+ N K ++GSFK+VAA
Sbjct: 1   MAASVSTVGAVNRAILNLNGSGAGASAPTSAFFGTSLKKAVASRVPNSKVTNGSFKIVAA 60

Query: 61  DSVPDENLDKQTSKDRWRGLGYDQSDDQQDITRGKGMVDTLFQAPMQSGTHYAVMSSYEY 120
           +   +E+  +QT+KDRW+GL YD SDDQQDITRGKGMVD LFQAPM +GTHYAVMSSYEY
Sbjct: 61  EKEIEES--QQTNKDRWKGLAYDISDDQQDITRGKGMVDPLFQAPMDAGTHYAVMSSYEY 120

Query: 121 LSTGLKTYEGLDNLLDGYYIAPAFMDKLVIHITKNYMTLPNIKVPLILGLWGGKGQGKSF 180
           LSTGL+    LDN+ DG+YIAPAF+DKLV+HITKN+MTLPNIKVPLILG+WGGKGQGKSF
Sbjct: 121 LSTGLRQ---LDNIKDGFYIAPAFLDKLVVHITKNFMTLPNIKVPLILGIWGGKGQGKSF 180

Query: 181 QCELVFAKMGINPIMMSAGELESGNAGEPAKLIRQRYREAADIIKKGKMSCLFINDLDAG 240
           QCELVFAKMGINPIMMSAGELESGNAGEPAKLIRQRYREAAD+I KGKM  LFINDLDAG
Sbjct: 181 QCELVFAKMGINPIMMSAGELESGNAGEPAKLIRQRYREAADLIAKGKMCALFINDLDAG 240

Query: 241 AGRLGGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKEENPRVPIIVTGNDFSTLYAP 300
           AGRLGGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKEEN RVPIIVTGNDFSTLYAP
Sbjct: 241 AGRLGGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKEENARVPIIVTGNDFSTLYAP 300

Query: 301 LIRDGRMEKFYWAPTRDDRIGVCKGIFRTDNVADEDIVKLVDTFPGQSIDFFGALRARVY 360
           LIRDGRMEKFYWAPTRDDR+GVCKGIFRTD V +EDI KLVDTFPGQSIDFFGALRARVY
Sbjct: 301 LIRDGRMEKFYWAPTRDDRVGVCKGIFRTDGVPEEDITKLVDTFPGQSIDFFGALRARVY 360

Query: 361 DDEVRKWIIGVGVQSIGKKLVNSREPPPKFEQPTMTLAKLLEYGNMLVQEQENVKRVQLA 420
           DDEVRKWI GVGV + GKKLVNS+E PP F+QP M+L KLL+YGNMLVQEQENVKRVQLA
Sbjct: 361 DDEVRKWISGVGVDATGKKLVNSKEGPPTFDQPKMSLDKLLQYGNMLVQEQENVKRVQLA 420

Query: 421 DTYLNEAALGNANEDAIKRGTF 443
           D YLNEAALGNANEDAIK G+F
Sbjct: 421 DKYLNEAALGNANEDAIKSGSF 437

BLAST of Lsi04G008990 vs. ExPASy Swiss-Prot
Match: Q7X9A0 (Ribulose bisphosphate carboxylase/oxygenase activase 1, chloroplastic OS=Larrea tridentata OX=66636 GN=RCA1 PE=1 SV=1)

HSP 1 Score: 730.7 bits (1885), Expect = 9.8e-210
Identity = 367/443 (82.84%), Postives = 402/443 (90.74%), Query Frame = 0

Query: 1   MAASAASVGVVNHAPLSLNGSGS-ATSVPSSVFFGNSLKKVVNSRIVNPKASSGSFKVVA 60
           MAA+ ++VG VN APLSLNGSG+ A+ VPS+ FFG+SLKK   S    PKASSG+FK+VA
Sbjct: 1   MAAAYSTVGAVNRAPLSLNGSGARASLVPSTAFFGSSLKK---SAAKFPKASSGNFKIVA 60

Query: 61  ADSVPDENLDKQTSKDRWRGLGYDQSDDQQDITRGKGMVDTLFQAPMQSGTHYAVMSSYE 120
            +     + D+QT KD+W+GL YD SDDQQDITRGKGMVDTLFQAPMQSGTHYAVMSSY+
Sbjct: 61  QEI----SEDQQTDKDKWKGLAYDISDDQQDITRGKGMVDTLFQAPMQSGTHYAVMSSYD 120

Query: 121 YLSTGLKTYEGLDNLLDGYYIAPAFMDKLVIHITKNYMTLPNIKVPLILGLWGGKGQGKS 180
           Y+S GL+ Y  LDN +DG+YIAPAFMDKLV+HITKN+++LPNIK+PLILG+WGGKGQGKS
Sbjct: 121 YISQGLRQY-NLDNNMDGFYIAPAFMDKLVVHITKNFLSLPNIKIPLILGIWGGKGQGKS 180

Query: 181 FQCELVFAKMGINPIMMSAGELESGNAGEPAKLIRQRYREAADIIKKGKMSCLFINDLDA 240
           FQCELVFAKMGINPIMMSAGELESGNAGEPAKLIRQRYREAADIIKKGKM CLFINDLDA
Sbjct: 181 FQCELVFAKMGINPIMMSAGELESGNAGEPAKLIRQRYREAADIIKKGKMCCLFINDLDA 240

Query: 241 GAGRLGGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKEENPRVPIIVTGNDFSTLYA 300
           GAGR+GGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKEENPRVPIIVTGNDFSTLYA
Sbjct: 241 GAGRMGGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKEENPRVPIIVTGNDFSTLYA 300

Query: 301 PLIRDGRMEKFYWAPTRDDRIGVCKGIFRTDNVADEDIVKLVDTFPGQSIDFFGALRARV 360
           PLIRDGRMEKFYWAPTR+DRIGVCKGIFRTDNVAD+DIVKLVDTFPGQSIDFFGALRARV
Sbjct: 301 PLIRDGRMEKFYWAPTREDRIGVCKGIFRTDNVADDDIVKLVDTFPGQSIDFFGALRARV 360

Query: 361 YDDEVRKWIIGVGVQSIGKKLVNSREPPPKFEQPTMTLAKLLEYGNMLVQEQENVKRVQL 420
           Y DEVRKW+  VGV +IGKKLVNS+E PP FEQP MT+ KLL YG MLVQEQENVKRVQL
Sbjct: 361 YHDEVRKWVSEVGVDTIGKKLVNSKEGPPSFEQPKMTIDKLLGYGGMLVQEQENVKRVQL 420

Query: 421 ADTYLNEAALGNANEDAIKRGTF 443
           AD Y++EAALG+AN DAIKRGTF
Sbjct: 421 ADKYMSEAALGDANNDAIKRGTF 435

BLAST of Lsi04G008990 vs. ExPASy Swiss-Prot
Match: Q7X999 (Ribulose bisphosphate carboxylase/oxygenase activase 2, chloroplastic OS=Larrea tridentata OX=66636 GN=RCA2 PE=2 SV=1)

HSP 1 Score: 730.7 bits (1885), Expect = 9.8e-210
Identity = 365/443 (82.39%), Postives = 404/443 (91.20%), Query Frame = 0

Query: 1   MAASAASVGVVNHAPLSLNGSGS-ATSVPSSVFFGNSLKKVVNSRIVNPKASSGSFKVVA 60
           MAA+ ++VG VN APLSLNGSG+ A+ VPS+ FFG+SLKK   S    PKASSG+FK+VA
Sbjct: 1   MAAAYSTVGAVNRAPLSLNGSGARASLVPSTAFFGSSLKK---SAAKFPKASSGNFKIVA 60

Query: 61  ADSVPDENLDKQTSKDRWRGLGYDQSDDQQDITRGKGMVDTLFQAPMQSGTHYAVMSSYE 120
            +     + D+QT KD+W+GL YD SDDQQDITRGKGMVDTLFQAPMQSGTHYAVMSSY+
Sbjct: 61  QEI----SEDQQTDKDKWKGLAYDISDDQQDITRGKGMVDTLFQAPMQSGTHYAVMSSYD 120

Query: 121 YLSTGLKTYEGLDNLLDGYYIAPAFMDKLVIHITKNYMTLPNIKVPLILGLWGGKGQGKS 180
           Y+S GL+ Y  LDN +DG+YIAPAFMDKLV+HITKN+++LPNIK+PLILG+WGGKGQGKS
Sbjct: 121 YISQGLRQY-NLDNNMDGFYIAPAFMDKLVVHITKNFLSLPNIKIPLILGIWGGKGQGKS 180

Query: 181 FQCELVFAKMGINPIMMSAGELESGNAGEPAKLIRQRYREAADIIKKGKMSCLFINDLDA 240
           FQCELVFAKMGINPIMMSAGELESGNAGEPAKLIRQRYREAADIIKKGKM CLFINDLDA
Sbjct: 181 FQCELVFAKMGINPIMMSAGELESGNAGEPAKLIRQRYREAADIIKKGKMCCLFINDLDA 240

Query: 241 GAGRLGGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKEENPRVPIIVTGNDFSTLYA 300
           GAGR+GGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKEENPRVPIIVTGNDFSTLYA
Sbjct: 241 GAGRMGGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKEENPRVPIIVTGNDFSTLYA 300

Query: 301 PLIRDGRMEKFYWAPTRDDRIGVCKGIFRTDNVADEDIVKLVDTFPGQSIDFFGALRARV 360
           PLIRDGRMEKFYWAPTR+DRIGVCKGIFRTDNV +EDIVK+VD FPGQSIDFFGALRARV
Sbjct: 301 PLIRDGRMEKFYWAPTREDRIGVCKGIFRTDNVPEEDIVKVVDQFPGQSIDFFGALRARV 360

Query: 361 YDDEVRKWIIGVGVQSIGKKLVNSREPPPKFEQPTMTLAKLLEYGNMLVQEQENVKRVQL 420
           YDDEVRKW+  VGV +IGKKLVNS+E PP FEQP MT+ KLL+YGNMLV+EQENVKRVQL
Sbjct: 361 YDDEVRKWVSEVGVDTIGKKLVNSKEGPPTFEQPKMTIDKLLQYGNMLVEEQENVKRVQL 420

Query: 421 ADTYLNEAALGNANEDAIKRGTF 443
           AD Y++EAALG+AN+DAIKRGTF
Sbjct: 421 ADKYMSEAALGDANQDAIKRGTF 435

BLAST of Lsi04G008990 vs. ExPASy Swiss-Prot
Match: Q40281 (Ribulose bisphosphate carboxylase/oxygenase activase, chloroplastic OS=Malus domestica OX=3750 GN=RCA PE=2 SV=1)

HSP 1 Score: 722.6 bits (1864), Expect = 2.7e-207
Identity = 364/442 (82.35%), Postives = 395/442 (89.37%), Query Frame = 0

Query: 1   MAASAASVGVVNHAPLSLNGSGSATSVPSSVFFGNSLKKVVNSRIVNPKASSGSFKVVAA 60
           MA + +++G VN AP +LNGS S+ SVPSS F G+SLKK VNSR  N K SSGS ++VA+
Sbjct: 1   MATAVSTIGSVNRAPPNLNGSSSSASVPSSTFLGSSLKK-VNSRFTNSKVSSGSLRIVAS 60

Query: 61  DSVPDENLDKQTSKDRWRGLGYDQSDDQQDITRGKGMVDTLFQAPMQSGTHYAVMSSYEY 120
               DE  DKQT KDRW+GL +D SDDQQDITRGKG VD+LFQAP  SGTH+A+MSSYEY
Sbjct: 61  ---VDE--DKQTDKDRWKGLAFDTSDDQQDITRGKGKVDSLFQAPQGSGTHFAIMSSYEY 120

Query: 121 LSTGLKTYEGLDNLLDGYYIAPAFMDKLVIHITKNYMTLPNIKVPLILGLWGGKGQGKSF 180
           +STGL+ Y   DN +DGYYIAPAFMDKLV+HITKN+MTLPN+KVPLILG+WGGKGQGKSF
Sbjct: 121 ISTGLRQY-NFDNNMDGYYIAPAFMDKLVVHITKNFMTLPNMKVPLILGIWGGKGQGKSF 180

Query: 181 QCELVFAKMGINPIMMSAGELESGNAGEPAKLIRQRYREAADIIKKGKMSCLFINDLDAG 240
           QCELVFAKM I+PIMMSAGELESGNAGEPAKLIRQRYREAADII+KGKM  LFINDLDAG
Sbjct: 181 QCELVFAKMRISPIMMSAGELESGNAGEPAKLIRQRYREAADIIRKGKMCALFINDLDAG 240

Query: 241 AGRLGGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKEENPRVPIIVTGNDFSTLYAP 300
           AGRLGGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKEENPRVPIIVTGNDFSTLYAP
Sbjct: 241 AGRLGGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKEENPRVPIIVTGNDFSTLYAP 300

Query: 301 LIRDGRMEKFYWAPTRDDRIGVCKGIFRTDNVADEDIVKLVDTFPGQSIDFFGALRARVY 360
           LIRDGRMEKFYWAPTR+DRIGVC GIFR+DNVA EDIVKLVDTFPGQSIDFFGALRARVY
Sbjct: 301 LIRDGRMEKFYWAPTREDRIGVCIGIFRSDNVAKEDIVKLVDTFPGQSIDFFGALRARVY 360

Query: 361 DDEVRKWIIGVGVQSIGKKLVNSREPPPKFEQPTMTLAKLLEYGNMLVQEQENVKRVQLA 420
           DDEVRKWI GVGV SIGKKLVNS+E PP FEQP MT+ KLLEYGNMLVQEQENVKRVQLA
Sbjct: 361 DDEVRKWITGVGVDSIGKKLVNSKEGPPTFEQPKMTIEKLLEYGNMLVQEQENVKRVQLA 420

Query: 421 DTYLNEAALGNANEDAIKRGTF 443
           D YL+EAALG+AN DA+  GTF
Sbjct: 421 DKYLSEAALGDANSDAMNTGTF 435

BLAST of Lsi04G008990 vs. ExPASy Swiss-Prot
Match: O64981 (Ribulose bisphosphate carboxylase/oxygenase activase, chloroplastic OS=Phaseolus vulgaris OX=3885 GN=RCA1 PE=2 SV=1)

HSP 1 Score: 717.6 bits (1851), Expect = 8.5e-206
Identity = 358/442 (81.00%), Postives = 393/442 (88.91%), Query Frame = 0

Query: 1   MAASAASVGVVNHAPLSLNGSGSATSVPSSVFFGNSLKKVVNSRIVNPKASSGSFKVVAA 60
           MAAS ++VG VN   L+LNGSG   S PSS FFG SLKKV++SR+ N K +SGSFK+VAA
Sbjct: 1   MAASLSTVGAVNRTLLNLNGSGGGASGPSSAFFGTSLKKVISSRVPNSKLTSGSFKIVAA 60

Query: 61  DSVPDENLDKQTSKDRWRGLGYDQSDDQQDITRGKGMVDTLFQAPMQSGTHYAVMSSYEY 120
           D   +E   +QT  DRWRGL YD SDDQQDITRGKG+VD+LFQAPM +GTHYAV+SS++Y
Sbjct: 61  DKEIEET--QQTEGDRWRGLAYDVSDDQQDITRGKGLVDSLFQAPMDAGTHYAVISSHKY 120

Query: 121 LSTGLKTYEGLDNLLDGYYIAPAFMDKLVIHITKNYMTLPNIKVPLILGLWGGKGQGKSF 180
           LS GL+ Y   DN+ DG+YIAPAF+DKLV+HI KN+MTLPNIKVPLILG+WGGKGQGKSF
Sbjct: 121 LSAGLRQY-NFDNIKDGFYIAPAFLDKLVVHIAKNFMTLPNIKVPLILGVWGGKGQGKSF 180

Query: 181 QCELVFAKMGINPIMMSAGELESGNAGEPAKLIRQRYREAADIIKKGKMSCLFINDLDAG 240
           QCELVFAKMGINPIMMSAGELESGNAGEPAKLIRQRYREA+D+IKKGKM  LFINDLDAG
Sbjct: 181 QCELVFAKMGINPIMMSAGELESGNAGEPAKLIRQRYREASDLIKKGKMCVLFINDLDAG 240

Query: 241 AGRLGGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKEENPRVPIIVTGNDFSTLYAP 300
           AGRLGGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKE+N RVPIIVTGNDFSTLYAP
Sbjct: 241 AGRLGGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKEDNARVPIIVTGNDFSTLYAP 300

Query: 301 LIRDGRMEKFYWAPTRDDRIGVCKGIFRTDNVADEDIVKLVDTFPGQSIDFFGALRARVY 360
           LIRDGRMEKFYWAPTR+DRIGVCKGIFRTD V ++DIV+LVD  PGQSIDFFGALRARVY
Sbjct: 301 LIRDGRMEKFYWAPTREDRIGVCKGIFRTDGVPEKDIVELVDKHPGQSIDFFGALRARVY 360

Query: 361 DDEVRKWIIGVGVQSIGKKLVNSREPPPKFEQPTMTLAKLLEYGNMLVQEQENVKRVQLA 420
           DDEVRKWI GVGV S+GKKLVNS+E PP F+QP MTL KLL Y +MLVQEQENVKRVQLA
Sbjct: 361 DDEVRKWISGVGVDSVGKKLVNSKEGPPTFDQPKMTLDKLLLYASMLVQEQENVKRVQLA 420

Query: 421 DTYLNEAALGNANEDAIKRGTF 443
           D YLNEAALGNANEDAIK G+F
Sbjct: 421 DQYLNEAALGNANEDAIKSGSF 439

BLAST of Lsi04G008990 vs. ExPASy TrEMBL
Match: A0A5A7U526 (Ribulose bisphosphate carboxylase/oxygenase activase 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold293G00550 PE=4 SV=1)

HSP 1 Score: 832.4 bits (2149), Expect = 8.8e-238
Identity = 413/442 (93.44%), Postives = 428/442 (96.83%), Query Frame = 0

Query: 1   MAASAASVGVVNHAPLSLNGSGSATSVPSSVFFGNSLKKVVNSRIVNPKASSGSFKVVAA 60
           MAASAASVGVVNHAPLSLNGSGSATSVPSSVFFG   KKVV SR V PKASSGSFKVVA 
Sbjct: 50  MAASAASVGVVNHAPLSLNGSGSATSVPSSVFFG---KKVVTSRAVTPKASSGSFKVVAV 109

Query: 61  DSVPDENLDKQTSKDRWRGLGYDQSDDQQDITRGKGMVDTLFQAPMQSGTHYAVMSSYEY 120
           +S+PDENL+KQT+KDRW GLGYD SDDQQDITRGKGMVD+LFQAPMQSGTHYAVMSSYEY
Sbjct: 110 ESIPDENLEKQTNKDRWAGLGYDISDDQQDITRGKGMVDSLFQAPMQSGTHYAVMSSYEY 169

Query: 121 LSTGLKTYEGLDNLLDGYYIAPAFMDKLVIHITKNYMTLPNIKVPLILGLWGGKGQGKSF 180
           LSTGLKTYEG+DN+LDGYYIAPAFMDKLV+HITKNYMTLPNIKVPLILGLWGGKGQGKSF
Sbjct: 170 LSTGLKTYEGMDNILDGYYIAPAFMDKLVVHITKNYMTLPNIKVPLILGLWGGKGQGKSF 229

Query: 181 QCELVFAKMGINPIMMSAGELESGNAGEPAKLIRQRYREAADIIKKGKMSCLFINDLDAG 240
           QCELVFAKMGINPIMMSAGELESGNAGEPAKLIRQRYREAADIIKKGKMSCLFINDLDAG
Sbjct: 230 QCELVFAKMGINPIMMSAGELESGNAGEPAKLIRQRYREAADIIKKGKMSCLFINDLDAG 289

Query: 241 AGRLGGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKEENPRVPIIVTGNDFSTLYAP 300
           AGRLGGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKEENPRVPIIVTGNDFSTLYAP
Sbjct: 290 AGRLGGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKEENPRVPIIVTGNDFSTLYAP 349

Query: 301 LIRDGRMEKFYWAPTRDDRIGVCKGIFRTDNVADEDIVKLVDTFPGQSIDFFGALRARVY 360
           LIRDGRMEKFYWAPTR+DRIGVCKGIFRTDNVADEDIVKLVDTFPGQSIDFFGALRARVY
Sbjct: 350 LIRDGRMEKFYWAPTREDRIGVCKGIFRTDNVADEDIVKLVDTFPGQSIDFFGALRARVY 409

Query: 361 DDEVRKWIIGVGVQSIGKKLVNSREPPPKFEQPTMTLAKLLEYGNMLVQEQENVKRVQLA 420
           DDEVRKW++ VGVQ+IGKKLVNS+EPPPKFEQP MTL KLLEYG+MLVQEQENVKRVQLA
Sbjct: 410 DDEVRKWVVAVGVQAIGKKLVNSKEPPPKFEQPAMTLQKLLEYGSMLVQEQENVKRVQLA 469

Query: 421 DTYLNEAALGNANEDAIKRGTF 443
           +TYLNEAALGNANEDAI RG F
Sbjct: 470 ETYLNEAALGNANEDAITRGAF 488

BLAST of Lsi04G008990 vs. ExPASy TrEMBL
Match: A0A1S3CF76 (ribulose bisphosphate carboxylase/oxygenase activase 2, chloroplastic-like OS=Cucumis melo OX=3656 GN=LOC103500234 PE=4 SV=1)

HSP 1 Score: 832.4 bits (2149), Expect = 8.8e-238
Identity = 413/442 (93.44%), Postives = 428/442 (96.83%), Query Frame = 0

Query: 1   MAASAASVGVVNHAPLSLNGSGSATSVPSSVFFGNSLKKVVNSRIVNPKASSGSFKVVAA 60
           MAASAASVGVVNHAPLSLNGSGSATSVPSSVFFG   KKVV SR V PKASSGSFKVVA 
Sbjct: 1   MAASAASVGVVNHAPLSLNGSGSATSVPSSVFFG---KKVVTSRAVTPKASSGSFKVVAV 60

Query: 61  DSVPDENLDKQTSKDRWRGLGYDQSDDQQDITRGKGMVDTLFQAPMQSGTHYAVMSSYEY 120
           +S+PDENL+KQT+KDRW GLGYD SDDQQDITRGKGMVD+LFQAPMQSGTHYAVMSSYEY
Sbjct: 61  ESIPDENLEKQTNKDRWAGLGYDISDDQQDITRGKGMVDSLFQAPMQSGTHYAVMSSYEY 120

Query: 121 LSTGLKTYEGLDNLLDGYYIAPAFMDKLVIHITKNYMTLPNIKVPLILGLWGGKGQGKSF 180
           LSTGLKTYEG+DN+LDGYYIAPAFMDKLV+HITKNYMTLPNIKVPLILGLWGGKGQGKSF
Sbjct: 121 LSTGLKTYEGMDNILDGYYIAPAFMDKLVVHITKNYMTLPNIKVPLILGLWGGKGQGKSF 180

Query: 181 QCELVFAKMGINPIMMSAGELESGNAGEPAKLIRQRYREAADIIKKGKMSCLFINDLDAG 240
           QCELVFAKMGINPIMMSAGELESGNAGEPAKLIRQRYREAADIIKKGKMSCLFINDLDAG
Sbjct: 181 QCELVFAKMGINPIMMSAGELESGNAGEPAKLIRQRYREAADIIKKGKMSCLFINDLDAG 240

Query: 241 AGRLGGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKEENPRVPIIVTGNDFSTLYAP 300
           AGRLGGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKEENPRVPIIVTGNDFSTLYAP
Sbjct: 241 AGRLGGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKEENPRVPIIVTGNDFSTLYAP 300

Query: 301 LIRDGRMEKFYWAPTRDDRIGVCKGIFRTDNVADEDIVKLVDTFPGQSIDFFGALRARVY 360
           LIRDGRMEKFYWAPTR+DRIGVCKGIFRTDNVADEDIVKLVDTFPGQSIDFFGALRARVY
Sbjct: 301 LIRDGRMEKFYWAPTREDRIGVCKGIFRTDNVADEDIVKLVDTFPGQSIDFFGALRARVY 360

Query: 361 DDEVRKWIIGVGVQSIGKKLVNSREPPPKFEQPTMTLAKLLEYGNMLVQEQENVKRVQLA 420
           DDEVRKW++ VGVQ+IGKKLVNS+EPPPKFEQP MTL KLLEYG+MLVQEQENVKRVQLA
Sbjct: 361 DDEVRKWVVAVGVQAIGKKLVNSKEPPPKFEQPAMTLQKLLEYGSMLVQEQENVKRVQLA 420

Query: 421 DTYLNEAALGNANEDAIKRGTF 443
           +TYLNEAALGNANEDAI RG F
Sbjct: 421 ETYLNEAALGNANEDAITRGAF 439

BLAST of Lsi04G008990 vs. ExPASy TrEMBL
Match: A0A6J1FN78 (ribulose bisphosphate carboxylase/oxygenase activase 2, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111446980 PE=4 SV=1)

HSP 1 Score: 822.8 bits (2124), Expect = 7.0e-235
Identity = 404/442 (91.40%), Postives = 427/442 (96.61%), Query Frame = 0

Query: 1   MAASAASVGVVNHAPLSLNGSGSATSVPSSVFFGNSLKKVVNSRIVNPKASSGSFKVVAA 60
           MAASAASVGV+NHAPLSLNGS SATSVPSSVFFG+SLKKVVNSRI +PKAS GSFKVVA+
Sbjct: 1   MAASAASVGVINHAPLSLNGSSSATSVPSSVFFGSSLKKVVNSRIASPKASPGSFKVVAS 60

Query: 61  DSVPDENLDKQTSKDRWRGLGYDQSDDQQDITRGKGMVDTLFQAPMQSGTHYAVMSSYEY 120
           D+  DE+LDKQTSKDRWRGL YD SDDQQDITRGKGMVDTLFQAPMQSGTH A+MSSYEY
Sbjct: 61  DAPADEDLDKQTSKDRWRGLAYDISDDQQDITRGKGMVDTLFQAPMQSGTHNAIMSSYEY 120

Query: 121 LSTGLKTYEGLDNLLDGYYIAPAFMDKLVIHITKNYMTLPNIKVPLILGLWGGKGQGKSF 180
           LSTGLKTYEGLDNLLDG+YIAPAFMDKLV+HITKN+M+LPNIKVPLILG+WGGKGQGKSF
Sbjct: 121 LSTGLKTYEGLDNLLDGFYIAPAFMDKLVVHITKNFMSLPNIKVPLILGIWGGKGQGKSF 180

Query: 181 QCELVFAKMGINPIMMSAGELESGNAGEPAKLIRQRYREAADIIKKGKMSCLFINDLDAG 240
           QCELVFAKMGI PIMMSAGELESGNAGEPAKLIRQRYREAADIIKKGKMSCLFINDLDAG
Sbjct: 181 QCELVFAKMGITPIMMSAGELESGNAGEPAKLIRQRYREAADIIKKGKMSCLFINDLDAG 240

Query: 241 AGRLGGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKEENPRVPIIVTGNDFSTLYAP 300
           AGRLGGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKE+NPRVPIIVTGNDFSTLYAP
Sbjct: 241 AGRLGGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKEDNPRVPIIVTGNDFSTLYAP 300

Query: 301 LIRDGRMEKFYWAPTRDDRIGVCKGIFRTDNVADEDIVKLVDTFPGQSIDFFGALRARVY 360
           LIRDGRMEKFYWAPTR+DRIGVCKGIFRTDNV DED+V+LVDTFPGQSIDFFGALRARVY
Sbjct: 301 LIRDGRMEKFYWAPTREDRIGVCKGIFRTDNVPDEDVVRLVDTFPGQSIDFFGALRARVY 360

Query: 361 DDEVRKWIIGVGVQSIGKKLVNSREPPPKFEQPTMTLAKLLEYGNMLVQEQENVKRVQLA 420
           DDEVRKWI+GVGV ++GKKLVNS++PPPKFEQP M++ KLLEYGNMLVQEQENVKRVQLA
Sbjct: 361 DDEVRKWIVGVGVDTVGKKLVNSKDPPPKFEQPKMSVEKLLEYGNMLVQEQENVKRVQLA 420

Query: 421 DTYLNEAALGNANEDAIKRGTF 443
           D YLNEAALGNANEDAIKRG F
Sbjct: 421 DKYLNEAALGNANEDAIKRGAF 442

BLAST of Lsi04G008990 vs. ExPASy TrEMBL
Match: A0A6J1K8Q9 (ribulose bisphosphate carboxylase/oxygenase activase 2, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111491148 PE=4 SV=1)

HSP 1 Score: 819.7 bits (2116), Expect = 5.9e-234
Identity = 403/442 (91.18%), Postives = 426/442 (96.38%), Query Frame = 0

Query: 1   MAASAASVGVVNHAPLSLNGSGSATSVPSSVFFGNSLKKVVNSRIVNPKASSGSFKVVAA 60
           MAASAASVGV+NHAPLSLNGS SATSVPSSVFFG+SLKKVVNSRI +PKAS GSFKVVA+
Sbjct: 1   MAASAASVGVINHAPLSLNGSSSATSVPSSVFFGSSLKKVVNSRIASPKASPGSFKVVAS 60

Query: 61  DSVPDENLDKQTSKDRWRGLGYDQSDDQQDITRGKGMVDTLFQAPMQSGTHYAVMSSYEY 120
           D+  DE+L+KQTSKDRWRGL YD SDDQQDITRGKGMVDTLFQAPMQSGTH A+MSSYEY
Sbjct: 61  DAPADEDLEKQTSKDRWRGLAYDISDDQQDITRGKGMVDTLFQAPMQSGTHNAIMSSYEY 120

Query: 121 LSTGLKTYEGLDNLLDGYYIAPAFMDKLVIHITKNYMTLPNIKVPLILGLWGGKGQGKSF 180
           LSTGLKTYEGLDNLLDG+YIAPAFMDKLV+HITKN+M+LPNIKVPLILG+WGGKGQGKSF
Sbjct: 121 LSTGLKTYEGLDNLLDGFYIAPAFMDKLVVHITKNFMSLPNIKVPLILGIWGGKGQGKSF 180

Query: 181 QCELVFAKMGINPIMMSAGELESGNAGEPAKLIRQRYREAADIIKKGKMSCLFINDLDAG 240
           QCELVFAKMGI PIMMSAGELESGNAGEPAKLIRQRYREAADIIKKGKMSCLFINDLDAG
Sbjct: 181 QCELVFAKMGITPIMMSAGELESGNAGEPAKLIRQRYREAADIIKKGKMSCLFINDLDAG 240

Query: 241 AGRLGGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKEENPRVPIIVTGNDFSTLYAP 300
           AGRLGGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKE+NPRVPIIVTGNDFSTLYAP
Sbjct: 241 AGRLGGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKEDNPRVPIIVTGNDFSTLYAP 300

Query: 301 LIRDGRMEKFYWAPTRDDRIGVCKGIFRTDNVADEDIVKLVDTFPGQSIDFFGALRARVY 360
           LIRDGRMEKFYWAPTR+DRIGVCKGIFRTDNV DED+V+LVDTFPGQSIDFFGALRARVY
Sbjct: 301 LIRDGRMEKFYWAPTREDRIGVCKGIFRTDNVPDEDVVRLVDTFPGQSIDFFGALRARVY 360

Query: 361 DDEVRKWIIGVGVQSIGKKLVNSREPPPKFEQPTMTLAKLLEYGNMLVQEQENVKRVQLA 420
           DDEVRKWIIGVGV ++GKKLVNS++PPPKFEQP M++ KLLEYGNMLVQEQENVKRVQLA
Sbjct: 361 DDEVRKWIIGVGVDTVGKKLVNSKDPPPKFEQPKMSVEKLLEYGNMLVQEQENVKRVQLA 420

Query: 421 DTYLNEAALGNANEDAIKRGTF 443
           D YLNEAALGNANEDAI RG F
Sbjct: 421 DKYLNEAALGNANEDAINRGAF 442

BLAST of Lsi04G008990 vs. ExPASy TrEMBL
Match: A0A0A0KLW9 (Beta-form rubisco activase OS=Cucumis sativus OX=3659 GN=Csa_5G182730 PE=4 SV=1)

HSP 1 Score: 806.6 bits (2082), Expect = 5.2e-230
Identity = 400/443 (90.29%), Postives = 422/443 (95.26%), Query Frame = 0

Query: 1   MAASAASVGVVNHAPLSLNGSGSATSVPSSVFFGNSLKKVVNSRIVNPKASSGSFKVVAA 60
           MAASAASVGVVNHAPLSL GSGS TSVPSSVFFGNSLKKVVNSR+VNPK SSGSFKV+A 
Sbjct: 1   MAASAASVGVVNHAPLSLKGSGSTTSVPSSVFFGNSLKKVVNSRVVNPKPSSGSFKVMAV 60

Query: 61  DSVPDENLDKQTSK-DRWRGLGYDQSDDQQDITRGKGMVDTLFQAPMQSGTHYAVMSSYE 120
           +S  DENL+K+T K DRW GLG D SDDQQDITRGKGMVD++FQAPMQ+GTHYAVMSSYE
Sbjct: 61  ESTSDENLEKKTKKIDRWAGLGTDISDDQQDITRGKGMVDSVFQAPMQAGTHYAVMSSYE 120

Query: 121 YLSTGLKTYEGLDNLLDGYYIAPAFMDKLVIHITKNYMTLPNIKVPLILGLWGGKGQGKS 180
           YLS G K+Y+G+DN+L G YIAPAFMDKLV+HITKN+++LPNIKVPLILGLWGGKGQGKS
Sbjct: 121 YLSQGRKSYDGMDNVLGGLYIAPAFMDKLVVHITKNFLSLPNIKVPLILGLWGGKGQGKS 180

Query: 181 FQCELVFAKMGINPIMMSAGELESGNAGEPAKLIRQRYREAADIIKKGKMSCLFINDLDA 240
           FQCELVFAKMGI PIMMSAGELESGNAGEPAKLIRQRYREAADIIKKGKMSCLFINDLDA
Sbjct: 181 FQCELVFAKMGITPIMMSAGELESGNAGEPAKLIRQRYREAADIIKKGKMSCLFINDLDA 240

Query: 241 GAGRLGGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKEENPRVPIIVTGNDFSTLYA 300
           GAGRLGGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKEENPRVPIIVTGNDFSTLYA
Sbjct: 241 GAGRLGGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKEENPRVPIIVTGNDFSTLYA 300

Query: 301 PLIRDGRMEKFYWAPTRDDRIGVCKGIFRTDNVADEDIVKLVDTFPGQSIDFFGALRARV 360
           PLIRDGRMEKFYWAPTR+DRIGVCKGIFRTDNVAD+DIVKLVDTFPGQSIDFFGALRARV
Sbjct: 301 PLIRDGRMEKFYWAPTREDRIGVCKGIFRTDNVADDDIVKLVDTFPGQSIDFFGALRARV 360

Query: 361 YDDEVRKWIIGVGVQSIGKKLVNSREPPPKFEQPTMTLAKLLEYGNMLVQEQENVKRVQL 420
           YDDEVRKW+ GVGVQSIGKKLVNS+EPPPKFEQPTM+L KLLEYG MLVQEQENVKRVQL
Sbjct: 361 YDDEVRKWVTGVGVQSIGKKLVNSKEPPPKFEQPTMSLEKLLEYGGMLVQEQENVKRVQL 420

Query: 421 ADTYLNEAALGNANEDAIKRGTF 443
           A+TYLNEAALGNANEDAI RG F
Sbjct: 421 AETYLNEAALGNANEDAITRGAF 443

BLAST of Lsi04G008990 vs. NCBI nr
Match: XP_038890850.1 (ribulose bisphosphate carboxylase/oxygenase activase, chloroplastic-like [Benincasa hispida])

HSP 1 Score: 860.1 bits (2221), Expect = 8.1e-246
Identity = 426/442 (96.38%), Postives = 436/442 (98.64%), Query Frame = 0

Query: 1   MAASAASVGVVNHAPLSLNGSGSATSVPSSVFFGNSLKKVVNSRIVNPKASSGSFKVVAA 60
           MAASAASVGVVNHAPLSLNGSGSATSVPSSVFFGNSLKKVVNSRIVN KASSGSFKVVAA
Sbjct: 1   MAASAASVGVVNHAPLSLNGSGSATSVPSSVFFGNSLKKVVNSRIVNQKASSGSFKVVAA 60

Query: 61  DSVPDENLDKQTSKDRWRGLGYDQSDDQQDITRGKGMVDTLFQAPMQSGTHYAVMSSYEY 120
           D++PDENL+KQTSKDRWRGLGYDQSDDQQDITRGKGMVDTLFQAPMQSGTHYAVMSSYEY
Sbjct: 61  DALPDENLEKQTSKDRWRGLGYDQSDDQQDITRGKGMVDTLFQAPMQSGTHYAVMSSYEY 120

Query: 121 LSTGLKTYEGLDNLLDGYYIAPAFMDKLVIHITKNYMTLPNIKVPLILGLWGGKGQGKSF 180
           LSTGLK+YEG+DNLLDGYYIAPAFMDKLV+HITKN+MTLPNIKVPLILGLWGGKGQGKSF
Sbjct: 121 LSTGLKSYEGMDNLLDGYYIAPAFMDKLVVHITKNFMTLPNIKVPLILGLWGGKGQGKSF 180

Query: 181 QCELVFAKMGINPIMMSAGELESGNAGEPAKLIRQRYREAADIIKKGKMSCLFINDLDAG 240
           QCELVFAKMGINPIMMSAGELESGNAGEPAKLIRQRYREAADIIKKGKMSCLFINDLDAG
Sbjct: 181 QCELVFAKMGINPIMMSAGELESGNAGEPAKLIRQRYREAADIIKKGKMSCLFINDLDAG 240

Query: 241 AGRLGGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKEENPRVPIIVTGNDFSTLYAP 300
           AGRLGGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKEENPRVPIIVTGNDFSTLYAP
Sbjct: 241 AGRLGGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKEENPRVPIIVTGNDFSTLYAP 300

Query: 301 LIRDGRMEKFYWAPTRDDRIGVCKGIFRTDNVADEDIVKLVDTFPGQSIDFFGALRARVY 360
           LIRDGRMEKFYWAPTR+DRIGVCKGIFRTDNVADEDIVKLVDTFPGQSIDFFGALRARVY
Sbjct: 301 LIRDGRMEKFYWAPTREDRIGVCKGIFRTDNVADEDIVKLVDTFPGQSIDFFGALRARVY 360

Query: 361 DDEVRKWIIGVGVQSIGKKLVNSREPPPKFEQPTMTLAKLLEYGNMLVQEQENVKRVQLA 420
           DDEVRKWI+GVGVQ +GKKLVNSREPPPKFEQP MTL KLLEYGNMLVQEQENVKRVQLA
Sbjct: 361 DDEVRKWIVGVGVQDVGKKLVNSREPPPKFEQPKMTLEKLLEYGNMLVQEQENVKRVQLA 420

Query: 421 DTYLNEAALGNANEDAIKRGTF 443
           DTYLNEAALGNANEDAI RG F
Sbjct: 421 DTYLNEAALGNANEDAISRGAF 442

BLAST of Lsi04G008990 vs. NCBI nr
Match: KAA0050344.1 (ribulose bisphosphate carboxylase/oxygenase activase 2 [Cucumis melo var. makuwa] >TYK03558.1 ribulose bisphosphate carboxylase/oxygenase activase 2 [Cucumis melo var. makuwa])

HSP 1 Score: 832.4 bits (2149), Expect = 1.8e-237
Identity = 413/442 (93.44%), Postives = 428/442 (96.83%), Query Frame = 0

Query: 1   MAASAASVGVVNHAPLSLNGSGSATSVPSSVFFGNSLKKVVNSRIVNPKASSGSFKVVAA 60
           MAASAASVGVVNHAPLSLNGSGSATSVPSSVFFG   KKVV SR V PKASSGSFKVVA 
Sbjct: 50  MAASAASVGVVNHAPLSLNGSGSATSVPSSVFFG---KKVVTSRAVTPKASSGSFKVVAV 109

Query: 61  DSVPDENLDKQTSKDRWRGLGYDQSDDQQDITRGKGMVDTLFQAPMQSGTHYAVMSSYEY 120
           +S+PDENL+KQT+KDRW GLGYD SDDQQDITRGKGMVD+LFQAPMQSGTHYAVMSSYEY
Sbjct: 110 ESIPDENLEKQTNKDRWAGLGYDISDDQQDITRGKGMVDSLFQAPMQSGTHYAVMSSYEY 169

Query: 121 LSTGLKTYEGLDNLLDGYYIAPAFMDKLVIHITKNYMTLPNIKVPLILGLWGGKGQGKSF 180
           LSTGLKTYEG+DN+LDGYYIAPAFMDKLV+HITKNYMTLPNIKVPLILGLWGGKGQGKSF
Sbjct: 170 LSTGLKTYEGMDNILDGYYIAPAFMDKLVVHITKNYMTLPNIKVPLILGLWGGKGQGKSF 229

Query: 181 QCELVFAKMGINPIMMSAGELESGNAGEPAKLIRQRYREAADIIKKGKMSCLFINDLDAG 240
           QCELVFAKMGINPIMMSAGELESGNAGEPAKLIRQRYREAADIIKKGKMSCLFINDLDAG
Sbjct: 230 QCELVFAKMGINPIMMSAGELESGNAGEPAKLIRQRYREAADIIKKGKMSCLFINDLDAG 289

Query: 241 AGRLGGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKEENPRVPIIVTGNDFSTLYAP 300
           AGRLGGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKEENPRVPIIVTGNDFSTLYAP
Sbjct: 290 AGRLGGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKEENPRVPIIVTGNDFSTLYAP 349

Query: 301 LIRDGRMEKFYWAPTRDDRIGVCKGIFRTDNVADEDIVKLVDTFPGQSIDFFGALRARVY 360
           LIRDGRMEKFYWAPTR+DRIGVCKGIFRTDNVADEDIVKLVDTFPGQSIDFFGALRARVY
Sbjct: 350 LIRDGRMEKFYWAPTREDRIGVCKGIFRTDNVADEDIVKLVDTFPGQSIDFFGALRARVY 409

Query: 361 DDEVRKWIIGVGVQSIGKKLVNSREPPPKFEQPTMTLAKLLEYGNMLVQEQENVKRVQLA 420
           DDEVRKW++ VGVQ+IGKKLVNS+EPPPKFEQP MTL KLLEYG+MLVQEQENVKRVQLA
Sbjct: 410 DDEVRKWVVAVGVQAIGKKLVNSKEPPPKFEQPAMTLQKLLEYGSMLVQEQENVKRVQLA 469

Query: 421 DTYLNEAALGNANEDAIKRGTF 443
           +TYLNEAALGNANEDAI RG F
Sbjct: 470 ETYLNEAALGNANEDAITRGAF 488

BLAST of Lsi04G008990 vs. NCBI nr
Match: XP_008461692.2 (PREDICTED: ribulose bisphosphate carboxylase/oxygenase activase 2, chloroplastic-like [Cucumis melo])

HSP 1 Score: 832.4 bits (2149), Expect = 1.8e-237
Identity = 413/442 (93.44%), Postives = 428/442 (96.83%), Query Frame = 0

Query: 1   MAASAASVGVVNHAPLSLNGSGSATSVPSSVFFGNSLKKVVNSRIVNPKASSGSFKVVAA 60
           MAASAASVGVVNHAPLSLNGSGSATSVPSSVFFG   KKVV SR V PKASSGSFKVVA 
Sbjct: 1   MAASAASVGVVNHAPLSLNGSGSATSVPSSVFFG---KKVVTSRAVTPKASSGSFKVVAV 60

Query: 61  DSVPDENLDKQTSKDRWRGLGYDQSDDQQDITRGKGMVDTLFQAPMQSGTHYAVMSSYEY 120
           +S+PDENL+KQT+KDRW GLGYD SDDQQDITRGKGMVD+LFQAPMQSGTHYAVMSSYEY
Sbjct: 61  ESIPDENLEKQTNKDRWAGLGYDISDDQQDITRGKGMVDSLFQAPMQSGTHYAVMSSYEY 120

Query: 121 LSTGLKTYEGLDNLLDGYYIAPAFMDKLVIHITKNYMTLPNIKVPLILGLWGGKGQGKSF 180
           LSTGLKTYEG+DN+LDGYYIAPAFMDKLV+HITKNYMTLPNIKVPLILGLWGGKGQGKSF
Sbjct: 121 LSTGLKTYEGMDNILDGYYIAPAFMDKLVVHITKNYMTLPNIKVPLILGLWGGKGQGKSF 180

Query: 181 QCELVFAKMGINPIMMSAGELESGNAGEPAKLIRQRYREAADIIKKGKMSCLFINDLDAG 240
           QCELVFAKMGINPIMMSAGELESGNAGEPAKLIRQRYREAADIIKKGKMSCLFINDLDAG
Sbjct: 181 QCELVFAKMGINPIMMSAGELESGNAGEPAKLIRQRYREAADIIKKGKMSCLFINDLDAG 240

Query: 241 AGRLGGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKEENPRVPIIVTGNDFSTLYAP 300
           AGRLGGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKEENPRVPIIVTGNDFSTLYAP
Sbjct: 241 AGRLGGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKEENPRVPIIVTGNDFSTLYAP 300

Query: 301 LIRDGRMEKFYWAPTRDDRIGVCKGIFRTDNVADEDIVKLVDTFPGQSIDFFGALRARVY 360
           LIRDGRMEKFYWAPTR+DRIGVCKGIFRTDNVADEDIVKLVDTFPGQSIDFFGALRARVY
Sbjct: 301 LIRDGRMEKFYWAPTREDRIGVCKGIFRTDNVADEDIVKLVDTFPGQSIDFFGALRARVY 360

Query: 361 DDEVRKWIIGVGVQSIGKKLVNSREPPPKFEQPTMTLAKLLEYGNMLVQEQENVKRVQLA 420
           DDEVRKW++ VGVQ+IGKKLVNS+EPPPKFEQP MTL KLLEYG+MLVQEQENVKRVQLA
Sbjct: 361 DDEVRKWVVAVGVQAIGKKLVNSKEPPPKFEQPAMTLQKLLEYGSMLVQEQENVKRVQLA 420

Query: 421 DTYLNEAALGNANEDAIKRGTF 443
           +TYLNEAALGNANEDAI RG F
Sbjct: 421 ETYLNEAALGNANEDAITRGAF 439

BLAST of Lsi04G008990 vs. NCBI nr
Match: XP_023529775.1 (ribulose bisphosphate carboxylase/oxygenase activase 2, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 823.9 bits (2127), Expect = 6.5e-235
Identity = 406/442 (91.86%), Postives = 426/442 (96.38%), Query Frame = 0

Query: 1   MAASAASVGVVNHAPLSLNGSGSATSVPSSVFFGNSLKKVVNSRIVNPKASSGSFKVVAA 60
           MAASAASVGV+NHAPLSLNGS SATSVPSSVFFG+SLKKVVNSRI +PKAS GSFKVVA+
Sbjct: 1   MAASAASVGVINHAPLSLNGSSSATSVPSSVFFGSSLKKVVNSRIASPKASPGSFKVVAS 60

Query: 61  DSVPDENLDKQTSKDRWRGLGYDQSDDQQDITRGKGMVDTLFQAPMQSGTHYAVMSSYEY 120
           D+  DE+LDKQTSKDRWRGL YD SDDQQDITRGKGMVDTLFQAPMQSGTH A+MSSYEY
Sbjct: 61  DAPADEDLDKQTSKDRWRGLAYDISDDQQDITRGKGMVDTLFQAPMQSGTHNAIMSSYEY 120

Query: 121 LSTGLKTYEGLDNLLDGYYIAPAFMDKLVIHITKNYMTLPNIKVPLILGLWGGKGQGKSF 180
           LSTGLKTYEGLDNLLDG+YIAPAFMDKLV+HITKN+M+LPNIKVPLILG+WGGKGQGKSF
Sbjct: 121 LSTGLKTYEGLDNLLDGFYIAPAFMDKLVVHITKNFMSLPNIKVPLILGIWGGKGQGKSF 180

Query: 181 QCELVFAKMGINPIMMSAGELESGNAGEPAKLIRQRYREAADIIKKGKMSCLFINDLDAG 240
           QCELVFAKMGI PIMMSAGELESGNAGEPAKLIRQRYREAADIIKKGKMSCLFINDLDAG
Sbjct: 181 QCELVFAKMGITPIMMSAGELESGNAGEPAKLIRQRYREAADIIKKGKMSCLFINDLDAG 240

Query: 241 AGRLGGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKEENPRVPIIVTGNDFSTLYAP 300
           AGRLGGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKE+NPRVPIIVTGNDFSTLYAP
Sbjct: 241 AGRLGGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKEDNPRVPIIVTGNDFSTLYAP 300

Query: 301 LIRDGRMEKFYWAPTRDDRIGVCKGIFRTDNVADEDIVKLVDTFPGQSIDFFGALRARVY 360
           LIRDGRMEKFYWAPTR+DRIGVCKGIFRTDNV DED+VKLVDTFPGQSIDFFGALRARVY
Sbjct: 301 LIRDGRMEKFYWAPTREDRIGVCKGIFRTDNVPDEDVVKLVDTFPGQSIDFFGALRARVY 360

Query: 361 DDEVRKWIIGVGVQSIGKKLVNSREPPPKFEQPTMTLAKLLEYGNMLVQEQENVKRVQLA 420
           DDEVRKWIIGVGV ++GKKLVNS++PPPKFEQP MT+ KLLEYGNMLVQEQENVKRVQLA
Sbjct: 361 DDEVRKWIIGVGVDTVGKKLVNSKDPPPKFEQPKMTVEKLLEYGNMLVQEQENVKRVQLA 420

Query: 421 DTYLNEAALGNANEDAIKRGTF 443
           D YLNEAALGNANEDAI RG F
Sbjct: 421 DKYLNEAALGNANEDAINRGAF 442

BLAST of Lsi04G008990 vs. NCBI nr
Match: XP_022941697.1 (ribulose bisphosphate carboxylase/oxygenase activase 2, chloroplastic-like [Cucurbita moschata])

HSP 1 Score: 822.8 bits (2124), Expect = 1.4e-234
Identity = 404/442 (91.40%), Postives = 427/442 (96.61%), Query Frame = 0

Query: 1   MAASAASVGVVNHAPLSLNGSGSATSVPSSVFFGNSLKKVVNSRIVNPKASSGSFKVVAA 60
           MAASAASVGV+NHAPLSLNGS SATSVPSSVFFG+SLKKVVNSRI +PKAS GSFKVVA+
Sbjct: 1   MAASAASVGVINHAPLSLNGSSSATSVPSSVFFGSSLKKVVNSRIASPKASPGSFKVVAS 60

Query: 61  DSVPDENLDKQTSKDRWRGLGYDQSDDQQDITRGKGMVDTLFQAPMQSGTHYAVMSSYEY 120
           D+  DE+LDKQTSKDRWRGL YD SDDQQDITRGKGMVDTLFQAPMQSGTH A+MSSYEY
Sbjct: 61  DAPADEDLDKQTSKDRWRGLAYDISDDQQDITRGKGMVDTLFQAPMQSGTHNAIMSSYEY 120

Query: 121 LSTGLKTYEGLDNLLDGYYIAPAFMDKLVIHITKNYMTLPNIKVPLILGLWGGKGQGKSF 180
           LSTGLKTYEGLDNLLDG+YIAPAFMDKLV+HITKN+M+LPNIKVPLILG+WGGKGQGKSF
Sbjct: 121 LSTGLKTYEGLDNLLDGFYIAPAFMDKLVVHITKNFMSLPNIKVPLILGIWGGKGQGKSF 180

Query: 181 QCELVFAKMGINPIMMSAGELESGNAGEPAKLIRQRYREAADIIKKGKMSCLFINDLDAG 240
           QCELVFAKMGI PIMMSAGELESGNAGEPAKLIRQRYREAADIIKKGKMSCLFINDLDAG
Sbjct: 181 QCELVFAKMGITPIMMSAGELESGNAGEPAKLIRQRYREAADIIKKGKMSCLFINDLDAG 240

Query: 241 AGRLGGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKEENPRVPIIVTGNDFSTLYAP 300
           AGRLGGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKE+NPRVPIIVTGNDFSTLYAP
Sbjct: 241 AGRLGGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKEDNPRVPIIVTGNDFSTLYAP 300

Query: 301 LIRDGRMEKFYWAPTRDDRIGVCKGIFRTDNVADEDIVKLVDTFPGQSIDFFGALRARVY 360
           LIRDGRMEKFYWAPTR+DRIGVCKGIFRTDNV DED+V+LVDTFPGQSIDFFGALRARVY
Sbjct: 301 LIRDGRMEKFYWAPTREDRIGVCKGIFRTDNVPDEDVVRLVDTFPGQSIDFFGALRARVY 360

Query: 361 DDEVRKWIIGVGVQSIGKKLVNSREPPPKFEQPTMTLAKLLEYGNMLVQEQENVKRVQLA 420
           DDEVRKWI+GVGV ++GKKLVNS++PPPKFEQP M++ KLLEYGNMLVQEQENVKRVQLA
Sbjct: 361 DDEVRKWIVGVGVDTVGKKLVNSKDPPPKFEQPKMSVEKLLEYGNMLVQEQENVKRVQLA 420

Query: 421 DTYLNEAALGNANEDAIKRGTF 443
           D YLNEAALGNANEDAIKRG F
Sbjct: 421 DKYLNEAALGNANEDAIKRGAF 442

BLAST of Lsi04G008990 vs. TAIR 10
Match: AT2G39730.1 (rubisco activase )

HSP 1 Score: 696.8 bits (1797), Expect = 1.1e-200
Identity = 354/445 (79.55%), Postives = 390/445 (87.64%), Query Frame = 0

Query: 1   MAASAASVGVVNHAPLSLNGSGS-ATSVPSSVFFGNSLKKVVN-SRIV-NPKASSGSFKV 60
           MAA+ ++VG +N APLSLNGSGS A S P+S F G   KKVV  SR   + K S+GSFKV
Sbjct: 1   MAAAVSTVGAINRAPLSLNGSGSGAVSAPASTFLG---KKVVTVSRFAQSNKKSNGSFKV 60

Query: 61  VAADSVPDENLDKQTSKDRWRGLGYDQSDDQQDITRGKGMVDTLFQAPMQSGTHYAVMSS 120
           +A         DKQT  DRWRGL YD SDDQQDITRGKGMVD++FQAPM +GTH+AV+SS
Sbjct: 61  LAVKE------DKQTDGDRWRGLAYDTSDDQQDITRGKGMVDSVFQAPMGTGTHHAVLSS 120

Query: 121 YEYLSTGLKTYEGLDNLLDGYYIAPAFMDKLVIHITKNYMTLPNIKVPLILGLWGGKGQG 180
           YEY+S GL+ Y  LDN++DG+YIAPAFMDKLV+HITKN++TLPNIKVPLILG+WGGKGQG
Sbjct: 121 YEYVSQGLRQY-NLDNMMDGFYIAPAFMDKLVVHITKNFLTLPNIKVPLILGIWGGKGQG 180

Query: 181 KSFQCELVFAKMGINPIMMSAGELESGNAGEPAKLIRQRYREAADIIKKGKMSCLFINDL 240
           KSFQCELV AKMGINPIMMSAGELESGNAGEPAKLIRQRYREAAD+IKKGKM CLFINDL
Sbjct: 181 KSFQCELVMAKMGINPIMMSAGELESGNAGEPAKLIRQRYREAADLIKKGKMCCLFINDL 240

Query: 241 DAGAGRLGGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKEENPRVPIIVTGNDFSTL 300
           DAGAGR+GGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKEEN RVPII TGNDFSTL
Sbjct: 241 DAGAGRMGGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKEENARVPIICTGNDFSTL 300

Query: 301 YAPLIRDGRMEKFYWAPTRDDRIGVCKGIFRTDNVADEDIVKLVDTFPGQSIDFFGALRA 360
           YAPLIRDGRMEKFYWAPTR+DRIGVCKGIFRTD + DEDIV LVD FPGQSIDFFGALRA
Sbjct: 301 YAPLIRDGRMEKFYWAPTREDRIGVCKGIFRTDKIKDEDIVTLVDQFPGQSIDFFGALRA 360

Query: 361 RVYDDEVRKWIIGVGVQSIGKKLVNSREPPPKFEQPTMTLAKLLEYGNMLVQEQENVKRV 420
           RVYDDEVRK++  +GV+ IGK+LVNSRE PP FEQP MT  KL+EYGNMLV EQENVKRV
Sbjct: 361 RVYDDEVRKFVESLGVEKIGKRLVNSREGPPVFEQPEMTYEKLMEYGNMLVMEQENVKRV 420

Query: 421 QLADTYLNEAALGNANEDAIKRGTF 443
           QLA+TYL++AALG+AN DAI RGTF
Sbjct: 421 QLAETYLSQAALGDANADAIGRGTF 435

BLAST of Lsi04G008990 vs. TAIR 10
Match: AT2G39730.2 (rubisco activase )

HSP 1 Score: 696.8 bits (1797), Expect = 1.1e-200
Identity = 354/445 (79.55%), Postives = 390/445 (87.64%), Query Frame = 0

Query: 1   MAASAASVGVVNHAPLSLNGSGS-ATSVPSSVFFGNSLKKVVN-SRIV-NPKASSGSFKV 60
           MAA+ ++VG +N APLSLNGSGS A S P+S F G   KKVV  SR   + K S+GSFKV
Sbjct: 1   MAAAVSTVGAINRAPLSLNGSGSGAVSAPASTFLG---KKVVTVSRFAQSNKKSNGSFKV 60

Query: 61  VAADSVPDENLDKQTSKDRWRGLGYDQSDDQQDITRGKGMVDTLFQAPMQSGTHYAVMSS 120
           +A         DKQT  DRWRGL YD SDDQQDITRGKGMVD++FQAPM +GTH+AV+SS
Sbjct: 61  LAVKE------DKQTDGDRWRGLAYDTSDDQQDITRGKGMVDSVFQAPMGTGTHHAVLSS 120

Query: 121 YEYLSTGLKTYEGLDNLLDGYYIAPAFMDKLVIHITKNYMTLPNIKVPLILGLWGGKGQG 180
           YEY+S GL+ Y  LDN++DG+YIAPAFMDKLV+HITKN++TLPNIKVPLILG+WGGKGQG
Sbjct: 121 YEYVSQGLRQY-NLDNMMDGFYIAPAFMDKLVVHITKNFLTLPNIKVPLILGIWGGKGQG 180

Query: 181 KSFQCELVFAKMGINPIMMSAGELESGNAGEPAKLIRQRYREAADIIKKGKMSCLFINDL 240
           KSFQCELV AKMGINPIMMSAGELESGNAGEPAKLIRQRYREAAD+IKKGKM CLFINDL
Sbjct: 181 KSFQCELVMAKMGINPIMMSAGELESGNAGEPAKLIRQRYREAADLIKKGKMCCLFINDL 240

Query: 241 DAGAGRLGGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKEENPRVPIIVTGNDFSTL 300
           DAGAGR+GGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKEEN RVPII TGNDFSTL
Sbjct: 241 DAGAGRMGGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKEENARVPIICTGNDFSTL 300

Query: 301 YAPLIRDGRMEKFYWAPTRDDRIGVCKGIFRTDNVADEDIVKLVDTFPGQSIDFFGALRA 360
           YAPLIRDGRMEKFYWAPTR+DRIGVCKGIFRTD + DEDIV LVD FPGQSIDFFGALRA
Sbjct: 301 YAPLIRDGRMEKFYWAPTREDRIGVCKGIFRTDKIKDEDIVTLVDQFPGQSIDFFGALRA 360

Query: 361 RVYDDEVRKWIIGVGVQSIGKKLVNSREPPPKFEQPTMTLAKLLEYGNMLVQEQENVKRV 420
           RVYDDEVRK++  +GV+ IGK+LVNSRE PP FEQP MT  KL+EYGNMLV EQENVKRV
Sbjct: 361 RVYDDEVRKFVESLGVEKIGKRLVNSREGPPVFEQPEMTYEKLMEYGNMLVMEQENVKRV 420

Query: 421 QLADTYLNEAALGNANEDAIKRGTF 443
           QLA+TYL++AALG+AN DAI RGTF
Sbjct: 421 QLAETYLSQAALGDANADAIGRGTF 435

BLAST of Lsi04G008990 vs. TAIR 10
Match: AT2G39730.3 (rubisco activase )

HSP 1 Score: 696.8 bits (1797), Expect = 1.1e-200
Identity = 354/445 (79.55%), Postives = 390/445 (87.64%), Query Frame = 0

Query: 1   MAASAASVGVVNHAPLSLNGSGS-ATSVPSSVFFGNSLKKVVN-SRIV-NPKASSGSFKV 60
           MAA+ ++VG +N APLSLNGSGS A S P+S F G   KKVV  SR   + K S+GSFKV
Sbjct: 1   MAAAVSTVGAINRAPLSLNGSGSGAVSAPASTFLG---KKVVTVSRFAQSNKKSNGSFKV 60

Query: 61  VAADSVPDENLDKQTSKDRWRGLGYDQSDDQQDITRGKGMVDTLFQAPMQSGTHYAVMSS 120
           +A         DKQT  DRWRGL YD SDDQQDITRGKGMVD++FQAPM +GTH+AV+SS
Sbjct: 61  LAVKE------DKQTDGDRWRGLAYDTSDDQQDITRGKGMVDSVFQAPMGTGTHHAVLSS 120

Query: 121 YEYLSTGLKTYEGLDNLLDGYYIAPAFMDKLVIHITKNYMTLPNIKVPLILGLWGGKGQG 180
           YEY+S GL+ Y  LDN++DG+YIAPAFMDKLV+HITKN++TLPNIKVPLILG+WGGKGQG
Sbjct: 121 YEYVSQGLRQY-NLDNMMDGFYIAPAFMDKLVVHITKNFLTLPNIKVPLILGIWGGKGQG 180

Query: 181 KSFQCELVFAKMGINPIMMSAGELESGNAGEPAKLIRQRYREAADIIKKGKMSCLFINDL 240
           KSFQCELV AKMGINPIMMSAGELESGNAGEPAKLIRQRYREAAD+IKKGKM CLFINDL
Sbjct: 181 KSFQCELVMAKMGINPIMMSAGELESGNAGEPAKLIRQRYREAADLIKKGKMCCLFINDL 240

Query: 241 DAGAGRLGGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKEENPRVPIIVTGNDFSTL 300
           DAGAGR+GGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKEEN RVPII TGNDFSTL
Sbjct: 241 DAGAGRMGGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKEENARVPIICTGNDFSTL 300

Query: 301 YAPLIRDGRMEKFYWAPTRDDRIGVCKGIFRTDNVADEDIVKLVDTFPGQSIDFFGALRA 360
           YAPLIRDGRMEKFYWAPTR+DRIGVCKGIFRTD + DEDIV LVD FPGQSIDFFGALRA
Sbjct: 301 YAPLIRDGRMEKFYWAPTREDRIGVCKGIFRTDKIKDEDIVTLVDQFPGQSIDFFGALRA 360

Query: 361 RVYDDEVRKWIIGVGVQSIGKKLVNSREPPPKFEQPTMTLAKLLEYGNMLVQEQENVKRV 420
           RVYDDEVRK++  +GV+ IGK+LVNSRE PP FEQP MT  KL+EYGNMLV EQENVKRV
Sbjct: 361 RVYDDEVRKFVESLGVEKIGKRLVNSREGPPVFEQPEMTYEKLMEYGNMLVMEQENVKRV 420

Query: 421 QLADTYLNEAALGNANEDAIKRGTF 443
           QLA+TYL++AALG+AN DAI RGTF
Sbjct: 421 QLAETYLSQAALGDANADAIGRGTF 435

BLAST of Lsi04G008990 vs. TAIR 10
Match: AT1G73110.1 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )

HSP 1 Score: 326.2 bits (835), Expect = 3.9e-89
Identity = 184/418 (44.02%), Postives = 260/418 (62.20%), Query Frame = 0

Query: 24  ATSVPSSVFFGNSLKKVVNSRIVNPKASSGSFKVVAAD---SVPDENLDKQTS------- 83
           ++S  SS F    +     S IV  K SS     VA D   + P + L +Q+S       
Sbjct: 17  SSSSSSSSFNATLINTRKLSSIVCSKPSSNDGGKVANDDGGAKPRKKLSEQSSWEVKDSE 76

Query: 84  -KDRWRGLGYDQSDDQQDITRGKGMVDTLFQAPMQSGTHYAVMSSYEYLSTGLKTYEGLD 143
            KD    LG +  +    +    GM+D +F           V   ++Y     +++E   
Sbjct: 77  GKDYLYRLGAESDNVNIAVGARSGMIDDVFIGDFLGKDSDIV---FDYRQKATRSFE--- 136

Query: 144 NLLDGYYIAPAFMDKLVIHITKNYMTLP-NIKVPLILGLWGGKGQGKSFQCELVFAKMGI 203
           +L   YYIAP+F+DK+ +HI KNY+    NIK+PLILG+WGGKGQGK+FQ EL+F  MG+
Sbjct: 137 HLQGDYYIAPSFLDKVAVHIVKNYLAPSLNIKIPLILGIWGGKGQGKTFQTELIFKTMGV 196

Query: 204 NPIMMSAGELESGNAGEPAKLIRQRYREAADIIK-KGKMSCLFINDLDAGAGRLGGTTQY 263
            P++MSAGELES  AGEP +LIR RYR A+ +I+ +GKMS L IND+DAG GR  G TQ 
Sbjct: 197 EPVIMSAGELESDRAGEPGRLIRDRYRTASQVIQNQGKMSVLMINDIDAGLGRF-GETQM 256

Query: 264 TVNNQMVNATLMNIADNPTNVQLPGMYNKEEN-PRVPIIVTGNDFSTLYAPLIRDGRMEK 323
           TVNNQ+V  TLMN+ADNPT V +   + + +   RVP+IVTGNDFSTLYAPLIR+GRMEK
Sbjct: 257 TVNNQIVVGTLMNLADNPTRVSVGQEWREADMVNRVPLIVTGNDFSTLYAPLIREGRMEK 316

Query: 324 FYWAPTRDDRIGVCKGIFRTDNVADEDIVKLVDTFPGQSIDFFGALRARVYDDEVRKWII 383
           FYW PTR+D + +   ++  D ++ +D++ +VD FP Q++DF+GALR+R YD  + KW+ 
Sbjct: 317 FYWQPTREDIVNIVSRMYEKDGISRKDVISIVDKFPNQALDFYGALRSRTYDRSILKWVD 376

Query: 384 GV-GVQSIGKKLVNSR--EPPPKFEQPTMTLAKLLEYGNMLVQEQENVKRVQLADTYL 425
              G++++GK L+  +  +  P+F  P  T+  LLE G  L+ EQ+ +   +L+  Y+
Sbjct: 377 EAGGMETLGKVLLRRKKTQEVPQFTAPEQTVEALLESGYSLINEQKLIMETKLSKEYM 427

BLAST of Lsi04G008990 vs. TAIR 10
Match: AT2G03670.1 (cell division cycle 48B )

HSP 1 Score: 49.7 bits (117), Expect = 7.1e-06
Identity = 40/163 (24.54%), Postives = 72/163 (44.17%), Query Frame = 0

Query: 162 IKVPLILGLWGGKGQGKSFQCELVFAKMGINPIMMSAGELESGNAGEPAKLIRQRYREAA 221
           +K P  L L+G  G GK+     V  +   + I++S   +   +AGE  K++R+ + EA+
Sbjct: 53  LKWPRGLLLYGPPGTGKTSLVRAVVQECDAHLIVLSPHSVHRAHAGESEKVLREAFAEAS 112

Query: 222 DIIKKGKMSCLFINDLDAGAGRLGGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKEE 281
                 K S +FI+++D    R     +  V       TLM+ ++ P++           
Sbjct: 113 SHAVSDKPSVIFIDEIDVLCPRRDARREQDVRIASQLFTLMD-SNKPSS----------S 172

Query: 282 NPRVPIIVTGNDFSTLYAPLIRDGRMEKF--YWAPTRDDRIGV 323
            PRV ++ + N    +   L R GR +       P  +DR+ +
Sbjct: 173 APRVVVVASTNRVDAIDPALRRAGRFDALVEVSTPNEEDRLKI 204

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O989975.2e-21182.81Ribulose bisphosphate carboxylase/oxygenase activase, chloroplastic OS=Vigna rad... [more]
Q7X9A09.8e-21082.84Ribulose bisphosphate carboxylase/oxygenase activase 1, chloroplastic OS=Larrea ... [more]
Q7X9999.8e-21082.39Ribulose bisphosphate carboxylase/oxygenase activase 2, chloroplastic OS=Larrea ... [more]
Q402812.7e-20782.35Ribulose bisphosphate carboxylase/oxygenase activase, chloroplastic OS=Malus dom... [more]
O649818.5e-20681.00Ribulose bisphosphate carboxylase/oxygenase activase, chloroplastic OS=Phaseolus... [more]
Match NameE-valueIdentityDescription
A0A5A7U5268.8e-23893.44Ribulose bisphosphate carboxylase/oxygenase activase 2 OS=Cucumis melo var. maku... [more]
A0A1S3CF768.8e-23893.44ribulose bisphosphate carboxylase/oxygenase activase 2, chloroplastic-like OS=Cu... [more]
A0A6J1FN787.0e-23591.40ribulose bisphosphate carboxylase/oxygenase activase 2, chloroplastic-like OS=Cu... [more]
A0A6J1K8Q95.9e-23491.18ribulose bisphosphate carboxylase/oxygenase activase 2, chloroplastic-like OS=Cu... [more]
A0A0A0KLW95.2e-23090.29Beta-form rubisco activase OS=Cucumis sativus OX=3659 GN=Csa_5G182730 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
XP_038890850.18.1e-24696.38ribulose bisphosphate carboxylase/oxygenase activase, chloroplastic-like [Beninc... [more]
KAA0050344.11.8e-23793.44ribulose bisphosphate carboxylase/oxygenase activase 2 [Cucumis melo var. makuwa... [more]
XP_008461692.21.8e-23793.44PREDICTED: ribulose bisphosphate carboxylase/oxygenase activase 2, chloroplastic... [more]
XP_023529775.16.5e-23591.86ribulose bisphosphate carboxylase/oxygenase activase 2, chloroplastic-like [Cucu... [more]
XP_022941697.11.4e-23491.40ribulose bisphosphate carboxylase/oxygenase activase 2, chloroplastic-like [Cucu... [more]
Match NameE-valueIdentityDescription
AT2G39730.11.1e-20079.55rubisco activase [more]
AT2G39730.21.1e-20079.55rubisco activase [more]
AT2G39730.31.1e-20079.55rubisco activase [more]
AT1G73110.13.9e-8944.02P-loop containing nucleoside triphosphate hydrolases superfamily protein [more]
AT2G03670.17.1e-0624.54cell division cycle 48B [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003959ATPase, AAA-type, corePFAMPF00004AAAcoord: 170..311
e-value: 1.3E-14
score: 54.7
NoneNo IPR availableGENE3D1.10.8.1070coord: 315..427
e-value: 2.5E-50
score: 171.1
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 67..89
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 66..89
NoneNo IPR availablePANTHERPTHR32429:SF19ATPASE, AAA-TYPE, CORE, P-LOOP CONTAINING NUCLEOSIDE TRIPHOSPHATE HYDROLASE-RELATEDcoord: 1..442
IPR027417P-loop containing nucleoside triphosphate hydrolaseGENE3D3.40.50.300coord: 130..314
e-value: 1.3E-43
score: 150.7
IPR027417P-loop containing nucleoside triphosphate hydrolaseSUPERFAMILY52540P-loop containing nucleoside triphosphate hydrolasescoord: 134..346
IPR044960Ribulose bisphosphate carboxylase/oxygenase activase-likePANTHERPTHR32429FAMILY NOT NAMEDcoord: 1..442

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi04G008990.1Lsi04G008990.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0050790 regulation of catalytic activity
molecular_function GO:0005524 ATP binding
molecular_function GO:0016887 ATP hydrolysis activity
molecular_function GO:0046863 ribulose-1,5-bisphosphate carboxylase/oxygenase activator activity