Lsi04G008730 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi04G008730
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionWAT1-related protein
Locationchr04: 8539635 .. 8546066 (-)
RNA-Seq ExpressionLsi04G008730
SyntenyLsi04G008730
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATAATTGTTTGGATGAATATCCTTTTAACCAATCCCTTTCTTGTCTCTTCCTTGCCTTTGATTGAGTTGGAAGTCCCTAAAACTTTGCTTATAAAACCATGTCAAATTTGAGGCCTTCTCCATGGTGTGTGTGTATTGTTATTCATATCAATTAGCTAAAGGCTTTGAACACGCACAAACAAGAGAGAGAGAAAGAAAGAAATGGAGGGTTTTGTGAAGTTTTTGGGTTTGGCAAAGCCATATTTTGGAGTTCTTTTTATGCAATTTGGTTCAGCTGGAATGGCCATCCTTGCAAAGTTTGCTTTGAACAAAGGGATGAGCCAATATGTTTTTGTGTTTTATCGTATGATTATTGCTACTCTTATCATCGCTCCTTTTGCCATCATCTTTGAAAGGTCTTTTATTATCCCTTTCTTTCTCTTTATATGTCTATCTACGTACGTACTTACCAACAATATGGTTGTTAGTTGAAAAATACTTAACTGTATCTTGTGTTACACTTCATCTTTCGTGTAGCCTATTTAATTGTCCAAAATCTTTTCAATCTTTTTTTTTGTGTGTGTGTGTGATTAGAGTGGCTGATCGCATAAAGATGAGTGCAGTCCTAGATACTTGAGTATTGCTTATTGTTATTTTGTACTCGTGGAATAAATATGGTCGATTTTTTTTGTTTTCTAGACTAAATAAACTAGTCGAGTTGTTTGTTTAGGTAATTTTCAATTTTGTCTTGATAATGTATTAGATATTTACAATATTTCTTTTAATATACTAAGATATGGTGGGTTGAGTATTAATAATATACGCGTTGGGTTGGTTTGTGTCGAAGAAGAATAATGTTTGAGTGGTATTTATTACAAGCATCTAAATATATTAACAAGTGTCAAAGAAGAATAATGTTTGAGTGGTATTTATTACCAGCATCTAAATATATTAACAAACTCGCAATATTTTAGTTATTTATTTTTTGTATTTGAAATTTATGTTTATTTTCCTTTAATATGTTAGTTATGTTTTCACCTTTTTTAAATTCAAACTTCAACTTTTCTAGAAAAGTTTTAACTAACAAAAACAAGTTTTAATTTTGTAAACTACAGATTTATCTAATAACCCTTTGATTTTAGGTTGTTTGTTTTTTAATTTTGTATTTATTTCGCACCAAAAAATGATTTTCATTATTTCCAAACAAAACTGTTATTTCTTATCAAATTTTCAAAAATAATACTACATTTTTTAGTTTTTAAAAATTTGATTTTTAGTTTTTGAAAACACTAACAAGTAATTCTATAACAAAGAAACTTAGGTACAAGTAGTGTTTATATGCTTAATTTTTAAAAACTAGAAAACCAACATAAATATAAAAATTTATAGATGGAAGTTAGTATTTACCCGCTTAATTTTCAAAACTAAAAAATAAACACAAAAAAAAACAAATATTTTTCGAACAAGATATTAATGATACCTTGTTATGGTTATGTTTTGAATTGAATGGCAATAAAAAGTAAAGCAAATTCAATAAACTATTTTTTTATTTTTTTATTTTTTTATTTTTTTTTACTTTCTTTCTCTCATTTTATAGAAGATTTGAGTTTTTAGTTTTGCATGCAGGAAAGTGAAAACGAAGATGACTATTCCGTTGGTCTTCAAGATTGTGATGCTGGGTTTATTGGAGTAAGAAAGGCTCATCATTTCATCACCATTAATATTAATATTAATATTAAACCACTGCTAATCTCACAAGTTTAAGTTAATTACAACCAAATTAATTCATTTGTTTCTTTATATTCTTAATTAACATCTAAACAAGTCAAAAAATTTAAATTCCCATGTATACGTGTTGTTATACTAATAAAAATAACATCATATTTTAAATTTTAAAAAACACTTTTGATTCTTAAAGTTTTTTAGAAATAACAATTTAGTCTTTATACTTTTAAATTTGTAACAATTTAGTCAATCAACTTTAACGAGTAACAATTTTAGTTTCTATACTTTAAAATTTTTAACAATTTATTTCTTTTCATGAATTTTTTTTATCAAAATTAAGTTCCAACTTTTATTAAGTAACAACTTAGTCTTTATAATTTACACATAAAATTGATTATCGATCAATTTATTGATCTATATGAGTAAGAAATTTTATTAAATCATAATAATATATACTTTTTACAATAAGAAGTAAATCGTTGCAAATGGTAACCTACAGGGACTATATTATTCCTTATTAAAGTTTAAGGATTCAATTGTTACAAACTTGAAAGTACAGAAACCAGAATTATTATCGACTAGTTTTCATGGACTAAATTGTTGTTTTCATAAAAGCTTAAGGACCAAAAGTGTTTCTTAACCTATTTTACCGATAAATTACAAAAAAAAAAAAAATATTTTTATTAAGGTTGTTGAGCCTAAAACACAAGGGGTTTATTTTTGTATGATAGGCCAGTGATTGATCTGAACTTATACTTTACTGGAATGAAGTATACAACAGCAACCTTTGCAGTTGCCATGTGCAATGTTCTACCAGCTTTTGCTTTCCTCATGGCTTGGGCTTGCAGGTACTTTCACTATCAAAACTTTCATTCATCTAATACATCATGAAGGCCTTTGAATTTCTTTTCTTTTTTTTTTTTTCGAGTTTAATACGTGTAGTGGGATTTTGAACTTTCGACTCTTGTCGATATATTGAAAGTAATATGCTTTAATCAGTTGAACTTTAAAGAATTATTGGGACATTTTTATGAAATGTTTTTTGGTCTTGTGTGTTTGTTTTGATGTTTTGTGCATGAAAGCTTTTTCAAAATAACCCCTTTTTGTTGTCTCCCAAACATTTGTATTAAAAGTAGTATGTTTGGTCACTGTTGTAAACTTTTTATATTAATTTTAATAAAGACATGATGTATAGATATGTCACATGTGTTTTAGGGTTTTCAAACATATTCTAATTCTAATTTATGTTAAATAATAATTAGTTAATTAGGGAAAGACTTGAAACAATTTATGCGAAATTGAGGGATGAAAACCAAACTTAGAAACTATGATAGAACTATTTAGAAGTTTAGTAGTCAAATATGAATTAAGCATGAAAATTTAGAGAATATAATATGATTCCAACGCAAAACTATTTATAAATATAGAAAAATTTCACTGTCTATCCCGCAATAAACTTCTATCTATCGCTGATTTGAAATTTGGTTTATCTCTGATAGATAGAAGTATCGCGATTTATTGCTAATAAATGCTAAAAAAAAATTATATTTGTAAATAGTTTGATGTTTTTTTTATTTATAATAATTTTCCTTTTTAAAATTAGTTGATTGAATTAAATGATTTTTTTTTGTTAAAATACCTTTTTGGTTTTCATCCGAAATGTTTGTTCAATTTTGATCTCTATACTTTTAATTATCGACAATTTTAGTCCCTAAACTTTCAATAAATCTATTATAACTTTAAGATTTATTAAAAGTATAAGAACTAAAATGGGATCAGAGAGACTAAAATTGAATAAACTTCAAAGAAAATGGATCAAAATGGTATTTTTTTTTTTTTTTTTTTTTTTTTTTTGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGAGATAGAATATTCAGTCAATTTTATTACATACATTAAAATCCACTTTCACAAATATATAACTTTAAAGTGAATATCTTTTTTAGTGGGTTCAAATCCCACAGCTGTCTAATGTTTGGTAGTTGATAAAGTTCCAATCAAGTTGTAATCTCTCCTTTTTTTTTCCCCTACCTATAACTCCAAGAAGAAAAACAGATCAAACACTTTTTCCTTTTTGAAAATATAGCAACGGAATTATGAGGGTGGGGTTCAAACTTTCAAGTTTCAACCCATAAAACTTTTTATGGTGATTGTTGATAAACAAAGTGGGCAGAGGGAGAAAACTCAAGCAAGAGCTTAGAGAACAGGAATGGAGAAGAAATTATGCATAACAAAGACCTCAACTATATGATGTTTAAATTCTCCAAATTACATAAAAACTACACAATCCAGAATATAAGATTTATGACTTCAATTTCAAGAGCTTCAATGAAACATATATGGATAAATTATATGGCAGGCTTGAGAAAGTGAATATTTTGAAACGGGGAAGCCAAGCAAAAATCCTAGGAACCATAGTAACAGTTGGAGGAGCCATGATTATGACATTCATAAGAGGACCCATGTTGAATCTGCCATGGACAAAAGCTAACCACCCCTCTGCTTCTTCAACTGGTTCAGTAAATCACCAAAGCCAAATCAAGGGCTCCCTCATGATTGCAATTGGCTGCATTTGCCAGTCAGCCTTCATCATTCTTCAGGTAAAAAAAAAAATGCTTTTTTTTTGCTTTGTGTGGAATCAGAGTTGCAGATTGAATAAGAAGCTAATCTACTCGATGTCGATGGCTTAGGCAATTACATTGAAATTGTATCCGGCCGAGCTGTCTCTTACAGCATTGATATGTTTGGTGGCCACCATTGGAGGTTGTGTGGTGGCTTTGGTCATGGAGAGGGGGAATCCTTCTGCTTGGGCTTTGAATTTTGATAGCCAGCTTCTGTCTGTGGTTTATGCTGTAAGTATCTGTGTTTTGAAAGTTCAGTTGATTCCATGGGAATGAGAATGAAAAGTTGTAAGCTTTTTGCTATTGTGTTAGTTCAAACTGTTTTGAGAGTGAAATGTACACAATGTAATATAACAGTTATGTTTTGTTGTAGGGAGTAATATGTTCAGGGGTAACATATTATATTCAAGGAATGGTGATGCAAATAAAAGGGCCTGTTTTTGTCACTGCATTCAATCCTCTGTCCATGATTTTTGTAGCAATCATGAGCTCCTTCATCTTGTCTGAGATTATGTTGCTGGGAAGGTAAGAAGAAGGCTGCATTGTTCCAAAATTTCCAAACAAAACTGAAAGAACAATGCAATTATAGATCTTATAAGAGATGTCATTTCAGTTGCAAAATGGGATACAAGCTTGCTAAAACTTTCAATTCTTTTGTAGGATTATTGGAGCAGTGACCATAATCACTGGATTGTACCTGGTTTTGTGGGGCAAAAGAAAAGATCAGTTTTCAGTTAAATCAGACTGTGATAACATAGCAGATAGTAAGCAACAAATGACTGAAACAGGCGAGGCTTCGAAGACGGTTCAATTGAGTCAAGAATCCGCCTCAGTTTGATGTTGCCAAAGAGGAGACAAATTGATGACTTCTAAGCCACCTGTAATTGTCCTGGTATCTGTTATAATGTGTTTAAAGTACCCTGCATTTTCATTTCAACCAAAATGTATTTGCAACTTTTGCATTTGTGAAAGGCTATATTAATAATAGTTTCTGTTTTCGTTTGGAACCAAAGCCCTGCCACTGGTTTTTGAGCTTCAAAGAGCCAGATGGTTGTTTCATACTATTCTATTGTGCTAAAATGCTTCAATACAATTCTGCCAAGAAGGGGGATAATATAAAGAACAAAATGTAACATGAAAGGAAAAGCAGAAGGCATTCTGAGCAATGCACACTTGTGATCAAAATGATGTCTTCAAAAGTCGGCAGGTAGCTGACCCGACCAAATACTGCCTTCACCAATTGCCACTCATCCATATCATCTTTGATTGCAACTACTGGTTTACAATTTACTCACTTTAGCAATGTTTTGATTACAAATCCATCCTGTGATTAAATACCCTGGTAGGTATATCTTTGAGGTCTACAAGTGGTATAATACACGTATCAGCAAAATAAAATAATATGTAAA

mRNA sequence

ATAATTGTTTGGATGAATATCCTTTTAACCAATCCCTTTCTTGTCTCTTCCTTGCCTTTGATTGAGTTGGAAGTCCCTAAAACTTTGCTTATAAAACCATGTCAAATTTGAGGCCTTCTCCATGGTGTGTGTGTATTGTTATTCATATCAATTAGCTAAAGGCTTTGAACACGCACAAACAAGAGAGAGAGAAAGAAAGAAATGGAGGGTTTTGTGAAGTTTTTGGGTTTGGCAAAGCCATATTTTGGAGTTCTTTTTATGCAATTTGGTTCAGCTGGAATGGCCATCCTTGCAAAGTTTGCTTTGAACAAAGGGATGAGCCAATATGTTTTTGTGTTTTATCGTATGATTATTGCTACTCTTATCATCGCTCCTTTTGCCATCATCTTTGAAAGGAAAGTGAAAACGAAGATGACTATTCCGTTGGTCTTCAAGATTGTGATGCTGGGTTTATTGGAGCCAGTGATTGATCTGAACTTATACTTTACTGGAATGAAGTATACAACAGCAACCTTTGCAGTTGCCATGTGCAATGTTCTACCAGCTTTTGCTTTCCTCATGGCTTGGGCTTGCAGGCTTGAGAAAGTGAATATTTTGAAACGGGGAAGCCAAGCAAAAATCCTAGGAACCATAGTAACAGTTGGAGGAGCCATGATTATGACATTCATAAGAGGACCCATGTTGAATCTGCCATGGACAAAAGCTAACCACCCCTCTGCTTCTTCAACTGGTTCAGTAAATCACCAAAGCCAAATCAAGGGCTCCCTCATGATTGCAATTGGCTGCATTTGCCAGTCAGCCTTCATCATTCTTCAGGCAATTACATTGAAATTGTATCCGGCCGAGCTGTCTCTTACAGCATTGATATGTTTGGTGGCCACCATTGGAGGTTGTGTGGTGGCTTTGGTCATGGAGAGGGGGAATCCTTCTGCTTGGGCTTTGAATTTTGATAGCCAGCTTCTGTCTGTGGTTTATGCTGGAGTAATATGTTCAGGGGTAACATATTATATTCAAGGAATGGTGATGCAAATAAAAGGGCCTGTTTTTGTCACTGCATTCAATCCTCTGTCCATGATTTTTGTAGCAATCATGAGCTCCTTCATCTTGTCTGAGATTATGTTGCTGGGAAGGATTATTGGAGCAGTGACCATAATCACTGGATTGTACCTGGTTTTGTGGGGCAAAAGAAAAGATCAGTTTTCAGTTAAATCAGACTGTGATAACATAGCAGATAGTAAGCAACAAATGACTGAAACAGGCGAGGCTTCGAAGACGGTTCAATTGAGTCAAGAATCCGCCTCAGTTTGATGTTGCCAAAGAGGAGACAAATTGATGACTTCTAAGCCACCTGTAATTGTCCTGGTATCTGTTATAATGTGTTTAAAGTACCCTGCATTTTCATTTCAACCAAAATGTATTTGCAACTTTTGCATTTGTGAAAGGCTATATTAATAATAGTTTCTGTTTTCGTTTGGAACCAAAGCCCTGCCACTGGTTTTTGAGCTTCAAAGAGCCAGATGGTTGTTTCATACTATTCTATTGTGCTAAAATGCTTCAATACAATTCTGCCAAGAAGGGGGATAATATAAAGAACAAAATGTAACATGAAAGGAAAAGCAGAAGGCATTCTGAGCAATGCACACTTGTGATCAAAATGATGTCTTCAAAAGTCGGCAGGTAGCTGACCCGACCAAATACTGCCTTCACCAATTGCCACTCATCCATATCATCTTTGATTGCAACTACTGGTTTACAATTTACTCACTTTAGCAATGTTTTGATTACAAATCCATCCTGTGATTAAATACCCTGGTAGGTATATCTTTGAGGTCTACAAGTGGTATAATACACGTATCAGCAAAATAAAATAATATGTAAA

Coding sequence (CDS)

ATGGAGGGTTTTGTGAAGTTTTTGGGTTTGGCAAAGCCATATTTTGGAGTTCTTTTTATGCAATTTGGTTCAGCTGGAATGGCCATCCTTGCAAAGTTTGCTTTGAACAAAGGGATGAGCCAATATGTTTTTGTGTTTTATCGTATGATTATTGCTACTCTTATCATCGCTCCTTTTGCCATCATCTTTGAAAGGAAAGTGAAAACGAAGATGACTATTCCGTTGGTCTTCAAGATTGTGATGCTGGGTTTATTGGAGCCAGTGATTGATCTGAACTTATACTTTACTGGAATGAAGTATACAACAGCAACCTTTGCAGTTGCCATGTGCAATGTTCTACCAGCTTTTGCTTTCCTCATGGCTTGGGCTTGCAGGCTTGAGAAAGTGAATATTTTGAAACGGGGAAGCCAAGCAAAAATCCTAGGAACCATAGTAACAGTTGGAGGAGCCATGATTATGACATTCATAAGAGGACCCATGTTGAATCTGCCATGGACAAAAGCTAACCACCCCTCTGCTTCTTCAACTGGTTCAGTAAATCACCAAAGCCAAATCAAGGGCTCCCTCATGATTGCAATTGGCTGCATTTGCCAGTCAGCCTTCATCATTCTTCAGGCAATTACATTGAAATTGTATCCGGCCGAGCTGTCTCTTACAGCATTGATATGTTTGGTGGCCACCATTGGAGGTTGTGTGGTGGCTTTGGTCATGGAGAGGGGGAATCCTTCTGCTTGGGCTTTGAATTTTGATAGCCAGCTTCTGTCTGTGGTTTATGCTGGAGTAATATGTTCAGGGGTAACATATTATATTCAAGGAATGGTGATGCAAATAAAAGGGCCTGTTTTTGTCACTGCATTCAATCCTCTGTCCATGATTTTTGTAGCAATCATGAGCTCCTTCATCTTGTCTGAGATTATGTTGCTGGGAAGGATTATTGGAGCAGTGACCATAATCACTGGATTGTACCTGGTTTTGTGGGGCAAAAGAAAAGATCAGTTTTCAGTTAAATCAGACTGTGATAACATAGCAGATAGTAAGCAACAAATGACTGAAACAGGCGAGGCTTCGAAGACGGTTCAATTGAGTCAAGAATCCGCCTCAGTTTGA

Protein sequence

MEGFVKFLGLAKPYFGVLFMQFGSAGMAILAKFALNKGMSQYVFVFYRMIIATLIIAPFAIIFERKVKTKMTIPLVFKIVMLGLLEPVIDLNLYFTGMKYTTATFAVAMCNVLPAFAFLMAWACRLEKVNILKRGSQAKILGTIVTVGGAMIMTFIRGPMLNLPWTKANHPSASSTGSVNHQSQIKGSLMIAIGCICQSAFIILQAITLKLYPAELSLTALICLVATIGGCVVALVMERGNPSAWALNFDSQLLSVVYAGVICSGVTYYIQGMVMQIKGPVFVTAFNPLSMIFVAIMSSFILSEIMLLGRIIGAVTIITGLYLVLWGKRKDQFSVKSDCDNIADSKQQMTETGEASKTVQLSQESASV
Homology
BLAST of Lsi04G008730 vs. ExPASy Swiss-Prot
Match: O80638 (WAT1-related protein At2g39510 OS=Arabidopsis thaliana OX=3702 GN=At2g39510 PE=2 SV=1)

HSP 1 Score: 389.0 bits (998), Expect = 5.8e-107
Identity = 205/332 (61.75%), Postives = 259/332 (78.01%), Query Frame = 0

Query: 12  KPYFGVLFMQFGSAGMAILAKFALNKGMSQYVFVFYRMIIATLIIAPFAIIFERKVKTKM 71
           KP+  V+ +QFG AG++I+AKFALN+GMS +V   YR I+AT+ IAPFA   +RK++ KM
Sbjct: 7   KPFITVVSLQFGYAGLSIIAKFALNQGMSPHVLASYRHIVATIFIAPFAYFLDRKIRPKM 66

Query: 72  TIPLVFKIVMLGLLEPVIDLNLYFTGMKYTTATFAVAMCNVLPAFAFLMAWACRLEKVNI 131
           T+ + FKI++LGLLEP ID NLY+TGMKYT+ATF  AM NVLPAFAF+MAW  RLEKVN+
Sbjct: 67  TLSIFFKILLLGLLEPTIDQNLYYTGMKYTSATFTAAMTNVLPAFAFIMAWIFRLEKVNV 126

Query: 132 LKRGSQAKILGTIVTVGGAMIMTFIRGPMLNLPWTKAN--HPSASSTGSVNHQSQIKGSL 191
            K  SQAKILGTIVTVGGAM+MT ++GP++ LPW   +  H  +S+TG    Q   KG+ 
Sbjct: 127 KKIHSQAKILGTIVTVGGAMLMTVVKGPLIPLPWANPHDIHQDSSNTGV--KQDLTKGAS 186

Query: 192 MIAIGCICQSAFIILQAITLKLYPAELSLTALICLVATIGGCVVALVMERGNPSAWALNF 251
           +IAIGCIC + FI LQAITLK YP ELSLTA IC + +I   +VAL +ERGNPSAWA++ 
Sbjct: 187 LIAIGCICWAGFINLQAITLKSYPVELSLTAYICFLGSIESTIVALFIERGNPSAWAIHL 246

Query: 252 DSQLLSVVYAGVICSGVTYYIQGMVMQIKGPVFVTAFNPLSMIFVAIMSSFILSEIMLLG 311
           DS+LL+ VY GVICSG+ YY+QG++M+ +GPVFVTAFNPLSM+ VAI+ S IL+E+M LG
Sbjct: 247 DSKLLAAVYGGVICSGIGYYVQGVIMKTRGPVFVTAFNPLSMVIVAILGSIILAEVMFLG 306

Query: 312 RIIGAVTIITGLYLVLWGKRKDQFSVK-SDCD 341
           RI+GA+ I+ GLY VLWGK KD+ S   SD D
Sbjct: 307 RILGAIVIVLGLYSVLWGKSKDEPSSSFSDMD 336

BLAST of Lsi04G008730 vs. ExPASy Swiss-Prot
Match: Q9ZUS1 (WAT1-related protein At2g37460 OS=Arabidopsis thaliana OX=3702 GN=At2g37460 PE=2 SV=1)

HSP 1 Score: 353.6 bits (906), Expect = 2.7e-96
Identity = 185/321 (57.63%), Postives = 244/321 (76.01%), Query Frame = 0

Query: 11  AKPYFGVLFMQFGSAGMAILAKFALNKGMSQYVFVFYRMIIATLIIAPFAIIFERKVKTK 70
           A+P+  ++ +Q G AGM IL+K  LNKGMS YV V YR  +AT+++APFA  F++KV+ K
Sbjct: 13  ARPFISMVVLQVGLAGMDILSKAVLNKGMSNYVLVVYRHAVATIVMAPFAFYFDKKVRPK 72

Query: 71  MTIPLVFKIVMLGLLEPVIDLNLYFTGMKYTTATFAVAMCNVLPAFAFLMAWACRLEKVN 130
           MT+ + FKI +LGLLEPVID NLY+ GMKYTTATFA AM NVLPA  F++A+   LE+V 
Sbjct: 73  MTLMIFFKISLLGLLEPVIDQNLYYLGMKYTTATFATAMYNVLPAITFVLAYIFGLERVK 132

Query: 131 ILKRGSQAKILGTIVTVGGAMIMTFIRGPMLNLPWTKANHPSASSTGSVNHQSQIKGSLM 190
           +    S  K++GT+ TVGGAMIMT ++GP+L+L WTK    SA +T   +  S IKG+++
Sbjct: 133 LRCIRSTGKVVGTLATVGGAMIMTLVKGPVLDLFWTKG--VSAHNTAGTDIHSAIKGAVL 192

Query: 191 IAIGCICQSAFIILQAITLKLYPAELSLTALICLVATIGGCVVALVMERGNPSAWALNFD 250
           + IGC   + F+ILQAITL+ YPAELSLTA ICL+ TI G  VALVME+GNPSAWA+ +D
Sbjct: 193 VTIGCFSYACFMILQAITLRTYPAELSLTAWICLMGTIEGTAVALVMEKGNPSAWAIGWD 252

Query: 251 SQLLSVVYAGVICSGVTYYIQGMVMQIKGPVFVTAFNPLSMIFVAIMSSFILSEIMLLGR 310
           ++LL+  Y+G++CS + YY+ G+VM+ +GPVFVTAF+PL MI VAIMS+ I +E M LGR
Sbjct: 253 TKLLTATYSGIVCSALAYYVGGVVMKTRGPVFVTAFSPLCMIIVAIMSTIIFAEQMYLGR 312

Query: 311 IIGAVTIITGLYLVLWGKRKD 332
           ++GAV I  GLYLV+WGK KD
Sbjct: 313 VLGAVVICAGLYLVIWGKGKD 331

BLAST of Lsi04G008730 vs. ExPASy Swiss-Prot
Match: Q9SUF1 (WAT1-related protein At4g08290 OS=Arabidopsis thaliana OX=3702 GN=At4g08290 PE=2 SV=1)

HSP 1 Score: 316.6 bits (810), Expect = 3.7e-85
Identity = 161/333 (48.35%), Postives = 240/333 (72.07%), Query Frame = 0

Query: 1   MEGFVKFLGLAKPYFGVLFMQFGSAGMAILAKFALNKGMSQYVFVFYRMIIATLIIAPFA 60
           MEG    +   +PY  ++F+QFG+AG  I+    LN+G ++YV + YR ++A L++APFA
Sbjct: 1   MEGVSATMHKLRPYLLMIFLQFGAAGTYIVIMATLNQGQNRYVVIVYRNLVAALVLAPFA 60

Query: 61  IIFERKVKTKMTIPLVFKIVMLGLLEPVIDLNLYFTGMKYTTATFAVAMCNVLPAFAFLM 120
           +IFERKV+ KMT+ +++KI+ LG LEPV+D    + GM  T+AT+  A+ N+LP+  F++
Sbjct: 61  LIFERKVRPKMTLSVLWKIMALGFLEPVLDQGFGYLGMNMTSATYTSAIMNILPSVTFII 120

Query: 121 AWACRLEKVNILKRGSQAKILGTIVTVGGAMIMTFIRGPMLNLPWTKAN--HPSASSTGS 180
           AW  R+EKVNI +  S+AKI+GT+V +GGA++MT  +GP++ LPW+  N    +  +  S
Sbjct: 121 AWILRMEKVNIAEVRSKAKIIGTLVGLGGALVMTLYKGPLIPLPWSNPNMDQQNGHTNNS 180

Query: 181 VNHQSQIKGSLMIAIGCICQSAFIILQAITLKLYPAELSLTALICLVATIGGCVVALVME 240
            +H + + G+L+I +GC+  S F +LQ+IT+K YPA+LSL+ALICL   +    VALV+E
Sbjct: 181 QDHNNWVVGTLLILLGCVAWSGFYVLQSITIKTYPADLSLSALICLAGAVQSFAVALVVE 240

Query: 241 RGNPSAWALNFDSQLLSVVYAGVICSGVTYYIQGMVMQIKGPVFVTAFNPLSMIFVAIMS 300
           R +PS WA+ +D++L + +Y G++ SG+TYY+QGMVM+ +GPVFVTAFNPL MI VA+++
Sbjct: 241 R-HPSGWAVGWDARLFAPLYTGIVSSGITYYVQGMVMKTRGPVFVTAFNPLCMILVALIA 300

Query: 301 SFILSEIMLLGRIIGAVTIITGLYLVLWGKRKD 332
           SFIL E +  G +IG   I  GLY+V+WGK KD
Sbjct: 301 SFILHEQIHFGCVIGGAVIAAGLYMVVWGKGKD 332

BLAST of Lsi04G008730 vs. ExPASy Swiss-Prot
Match: F4IQX1 (WAT1-related protein At2g37450 OS=Arabidopsis thaliana OX=3702 GN=At2g37450 PE=2 SV=1)

HSP 1 Score: 301.2 bits (770), Expect = 1.6e-80
Identity = 168/321 (52.34%), Postives = 214/321 (66.67%), Query Frame = 0

Query: 11  AKPYFGVLFMQFGSAGMAILAKFALNKGMSQYVFVFYRMIIATLIIAPFAIIFERKVKTK 70
           A P+  ++ +Q G AGM IL K  LNKGMS YV   YR  +AT+++APFA  F+      
Sbjct: 7   ALPFILMVLLQIGYAGMDILTKDVLNKGMSIYVLSVYRHGVATVVMAPFAFYFD------ 66

Query: 71  MTIPLVFKIVMLGLLEPVIDLNLYFTGMKYTTATFAVAMCNVLPAFAFLMAWACRLEKVN 130
                           PVI  NL+  GMKYTTATFA+A+ N LPA  F++A   RLE V 
Sbjct: 67  ---------------NPVIAQNLFNLGMKYTTATFAIALYNTLPAVTFILALIFRLESVK 126

Query: 131 ILKRGSQAKILGTIVTVGGAMIMTFIRGPMLNLPWTKANHPSASSTGSVNHQSQIKGSLM 190
                S AK++GT+ TVGG M+MT ++GP L+L WTK   PSA +T   +  S IKG+++
Sbjct: 127 FQSIRSAAKVVGTVTTVGGIMVMTLVKGPALDLFWTKG--PSAQNTVGTDIHSSIKGAVL 186

Query: 191 IAIGCICQSAFIILQAITLKLYPAELSLTALICLVATIGGCVVALVMERGNPSAWALNFD 250
           + IGC   + F+ILQAITLK YPAELSL   ICL+ TI G VVALVME+GNPS WA+ +D
Sbjct: 187 VTIGCFSYACFMILQAITLKTYPAELSLATWICLIGTIEGVVVALVMEKGNPSVWAIGWD 246

Query: 251 SQLLSVVYAGVICSGVTYYIQGMVMQIKGPVFVTAFNPLSMIFVAIMSSFILSEIMLLGR 310
           ++LL++ Y+G++CS + YYI G+VM+ +GPVFVTAF PL MI VAIMSS I  E M LGR
Sbjct: 247 TKLLTITYSGIVCSALGYYIGGVVMKTRGPVFVTAFKPLCMIVVAIMSSIIFDEQMYLGR 304

Query: 311 IIGAVTIITGLYLVLWGKRKD 332
            +GA  I  GLYLV+WGK KD
Sbjct: 307 ALGATVICVGLYLVIWGKAKD 304

BLAST of Lsi04G008730 vs. ExPASy Swiss-Prot
Match: Q9FL41 (WAT1-related protein At5g07050 OS=Arabidopsis thaliana OX=3702 GN=At5g07050 PE=2 SV=1)

HSP 1 Score: 297.4 bits (760), Expect = 2.3e-79
Identity = 169/358 (47.21%), Postives = 237/358 (66.20%), Query Frame = 0

Query: 7   FLGLAKPYFGVLFMQFGSAGMAILAKFALNKGMSQYVFVFYRMIIATLIIAPFAIIFERK 66
           FL  +KPYF ++ +QFG AGM I+ K +LN GMS YV V YR  IAT +IAPFA  FERK
Sbjct: 12  FLTSSKPYFAMISLQFGYAGMNIITKISLNTGMSHYVLVVYRHAIATAVIAPFAFFFERK 71

Query: 67  VKTKMTIPLVFKIVMLGLLEPVIDLNLYFTGMKYTTATFAVAMCNVLPAFAFLMAWACRL 126
            + K+T  +  ++ +LGLL PVID N Y+ G+KYT+ TF+ AM N+LPA  F++A   R+
Sbjct: 72  AQPKITFSIFMQLFILGLLGPVIDQNFYYMGLKYTSPTFSCAMSNMLPAMTFILAVLFRM 131

Query: 127 EKVNILKRGSQAKILGTIVTVGGAMIMTFIRGPMLNLPWTKANH----PSASSTGSVNHQ 186
           E +++ K   QAKI GT+VTV GAM+MT  +GP++ L WTK  H      A++T S N  
Sbjct: 132 EMLDLKKLWCQAKIAGTVVTVAGAMLMTIYKGPIVELFWTKYMHIQDSSHANTTSSKNSS 191

Query: 187 SQ---IKGSLMIAIGCICQSAFIILQAITLKLYPA-ELSLTALICLVATIGGCVVALVME 246
           S    +KGS+++    +  ++  +LQA  LK Y   +LSLT LIC + T+    V  VME
Sbjct: 192 SDKEFLKGSILLIFATLAWASLFVLQAKILKTYAKHQLSLTTLICFIGTLQAVAVTFVME 251

Query: 247 RGNPSAWALNFDSQLLSVVYAGVICSGVTYYIQGMVMQIKGPVFVTAFNPLSMIFVAIMS 306
             NPSAW + +D  LL+  Y+G++ S ++YY+QG+VM+ +GPVF TAF+PL M+ VA+M 
Sbjct: 252 H-NPSAWRIGWDMNLLAAAYSGIVASSISYYVQGIVMKKRGPVFATAFSPLMMVIVAVMG 311

Query: 307 SFILSEIMLLGRIIGAVTIITGLYLVLWGKRKDQFSVKSDCDNIADSKQQMTETGEAS 357
           SF+L+E + LG +IGAV I+ GLY VLWGK+K+      +   I DS  ++TE  EA+
Sbjct: 312 SFVLAEKIFLGGVIGAVLIVIGLYAVLWGKQKENQVTICELAKI-DSNSKVTEDVEAN 367

BLAST of Lsi04G008730 vs. ExPASy TrEMBL
Match: A0A1S3CF33 (WAT1-related protein OS=Cucumis melo OX=3656 GN=LOC103500199 PE=3 SV=1)

HSP 1 Score: 612.5 bits (1578), Expect = 1.2e-171
Identity = 335/370 (90.54%), Postives = 349/370 (94.32%), Query Frame = 0

Query: 1   MEGFVKFLGLAKPYFGVLFMQFGSAGMAILAKFALNKGMSQYVFVFYRMIIATLIIAPFA 60
           MEGFV+FL LAKPYFGVLFMQ GSAGMAI+AKFALNKGMSQYVFVFYRMIIATLI+APFA
Sbjct: 1   MEGFVRFLVLAKPYFGVLFMQLGSAGMAIIAKFALNKGMSQYVFVFYRMIIATLIMAPFA 60

Query: 61  IIFERKVKTKMTIPLVFKIVMLGLLEPVIDLNLYFTGMKYTTATFAVAMCNVLPAFAFLM 120
           IIFERKV+TKMTI LVFKIVMLGLLEPVIDLNLYFTGMKYTTATFAVAMCNVLPAFAFLM
Sbjct: 61  IIFERKVRTKMTISLVFKIVMLGLLEPVIDLNLYFTGMKYTTATFAVAMCNVLPAFAFLM 120

Query: 121 AWACRLEKVNILKRGSQAKILGTIVTVGGAMIMTFIRGPMLNLPWTKANHP-SASSTGSV 180
           AWACRLEKVNI KRGSQAKI+GTIVTVGGAMIMTFIRGPMLNLPWTK NHP S+SS+ S 
Sbjct: 121 AWACRLEKVNIRKRGSQAKIVGTIVTVGGAMIMTFIRGPMLNLPWTKPNHPSSSSSSSSK 180

Query: 181 NHQSQIKGSLMIAIGCICQSAFIILQAITLKLYPAELSLTALICLVATIGGCVVALVMER 240
           NHQ QIKGSLMIAIGCICQSAFIILQAITLKLYPAELSLTALICLVAT+GGC+VALVMER
Sbjct: 181 NHQDQIKGSLMIAIGCICQSAFIILQAITLKLYPAELSLTALICLVATVGGCLVALVMER 240

Query: 241 GNPSAWALNFDSQLLSVVYAGVICSGVTYYIQGMVMQIKGPVFVTAFNPLSMIFVAIMSS 300
           GNPSAWAL+FDSQLLSVVYAGVICSGVTYYIQGMVMQIKGPVFVTAFNPLSMIFVAIMSS
Sbjct: 241 GNPSAWALHFDSQLLSVVYAGVICSGVTYYIQGMVMQIKGPVFVTAFNPLSMIFVAIMSS 300

Query: 301 FILSEIMLLGRIIGAVTIITGLYLVLWGKRKDQFSVKSDCDNIADSKQQMTETGEAS-KT 360
           FILSEIMLLGRI+GAVTIITGLYLVLWGKRKD+  VKSD    +DSKQQMTET E S KT
Sbjct: 301 FILSEIMLLGRIVGAVTIITGLYLVLWGKRKDRLLVKSD----SDSKQQMTETDETSKKT 360

Query: 361 VQLSQESASV 369
           VQ SQE  S+
Sbjct: 361 VQPSQEFISL 366

BLAST of Lsi04G008730 vs. ExPASy TrEMBL
Match: A0A0A0KS68 (WAT1-related protein OS=Cucumis sativus OX=3659 GN=Csa_5G181430 PE=3 SV=1)

HSP 1 Score: 601.7 bits (1550), Expect = 2.1e-168
Identity = 327/371 (88.14%), Postives = 346/371 (93.26%), Query Frame = 0

Query: 1   MEGFVKFLGLAKPYFGVLFMQFGSAGMAILAKFALNKGMSQYVFVFYRMIIATLIIAPFA 60
           MEG  +FL LAKPYFGVLFMQ GSAGMAI+AKFALNKGMSQYVFVFYRMIIAT+I+APFA
Sbjct: 1   MEGLRRFLVLAKPYFGVLFMQLGSAGMAIIAKFALNKGMSQYVFVFYRMIIATVIMAPFA 60

Query: 61  IIFERKVKTKMTIPLVFKIVMLGLLEPVIDLNLYFTGMKYTTATFAVAMCNVLPAFAFLM 120
           IIFERKV+TKMTI L FKIVMLGLLEPVIDLNLYFTGMKYTTATFAVAMCNVLPAFAFLM
Sbjct: 61  IIFERKVRTKMTISLAFKIVMLGLLEPVIDLNLYFTGMKYTTATFAVAMCNVLPAFAFLM 120

Query: 121 AWACRLEKVNILKRGSQAKILGTIVTVGGAMIMTFIRGPMLNLPWTKANHP--SASSTGS 180
           AWACRLE+VNI KRGSQAKI+GTIVT+GGAMIMTFIRGPMLNLPWTK NHP  S+SS+ S
Sbjct: 121 AWACRLERVNIWKRGSQAKIVGTIVTIGGAMIMTFIRGPMLNLPWTKPNHPSSSSSSSSS 180

Query: 181 VNHQSQIKGSLMIAIGCICQSAFIILQAITLKLYPAELSLTALICLVATIGGCVVALVME 240
           +NH +QIKGSLMI IGCICQSAFIILQAITLKLYPAELSLTALICLVAT+GGC+VALVME
Sbjct: 181 INHHNQIKGSLMITIGCICQSAFIILQAITLKLYPAELSLTALICLVATVGGCLVALVME 240

Query: 241 RGNPSAWALNFDSQLLSVVYAGVICSGVTYYIQGMVMQIKGPVFVTAFNPLSMIFVAIMS 300
           RGNPSAWAL+FDSQLLSVVYAGVICSGVTYYIQGMVMQIKGPVFVTAFNPLSMIFVAIMS
Sbjct: 241 RGNPSAWALHFDSQLLSVVYAGVICSGVTYYIQGMVMQIKGPVFVTAFNPLSMIFVAIMS 300

Query: 301 SFILSEIMLLGRIIGAVTIITGLYLVLWGKRKDQFSVKSDCDNIADSKQQMTETGEASK- 360
           SFILSEIMLLGRI+GAVTIITGLYLVLWGKRKD+  VKSD    +D KQQMTET EASK 
Sbjct: 301 SFILSEIMLLGRIVGAVTIITGLYLVLWGKRKDKLLVKSD----SDGKQQMTETDEASKR 360

Query: 361 TVQLSQESASV 369
           TVQ SQE  S+
Sbjct: 361 TVQPSQEFISL 367

BLAST of Lsi04G008730 vs. ExPASy TrEMBL
Match: A0A5A7U9L7 (WAT1-related protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold88G00140 PE=3 SV=1)

HSP 1 Score: 573.2 bits (1476), Expect = 8.0e-160
Identity = 311/370 (84.05%), Postives = 335/370 (90.54%), Query Frame = 0

Query: 1   MEGFVKFLGLAKPYFGVLFMQFGSAGMAILAKFALNKGMSQYVFVFYRMIIATLIIAPFA 60
           ME FV+F+  AKPYFGV+F+QFG AGM IL K AL+KGMSQ+VFV YR + ATL+IAPFA
Sbjct: 1   MESFVRFMRSAKPYFGVIFVQFGYAGMTILTKSALDKGMSQHVFVAYRQVAATLVIAPFA 60

Query: 61  IIFERKVKTKMTIPLVFKIVMLGLLEPVIDLNLYFTGMKYTTATFAVAMCNVLPAFAFLM 120
           IIFERK +TKMT  L+FKI++LG LEPVID NL++TGMKYTTATFA AMCNVLPAF FLM
Sbjct: 61  IIFERKARTKMTFSLLFKILLLGFLEPVIDQNLFYTGMKYTTATFAAAMCNVLPAFVFLM 120

Query: 121 AWACRLEKVNILKRGSQAKILGTIVTVGGAMIMTFIRGPMLNLPWTKANHP-SASSTGSV 180
           AWACRLEKVNI KRGSQAKI+GTIVTVGGAMIMTFIRGPMLNLPWTK NHP S+SS+ S 
Sbjct: 121 AWACRLEKVNIWKRGSQAKIVGTIVTVGGAMIMTFIRGPMLNLPWTKPNHPSSSSSSSSK 180

Query: 181 NHQSQIKGSLMIAIGCICQSAFIILQAITLKLYPAELSLTALICLVATIGGCVVALVMER 240
           NHQ QIKGSLMIAIGCICQSAFIILQAITLKLYPAELSLTALICLVAT+GGC+VALVMER
Sbjct: 181 NHQDQIKGSLMIAIGCICQSAFIILQAITLKLYPAELSLTALICLVATVGGCLVALVMER 240

Query: 241 GNPSAWALNFDSQLLSVVYAGVICSGVTYYIQGMVMQIKGPVFVTAFNPLSMIFVAIMSS 300
           GNPSAWAL+FDSQLLSVVYAGVICSGVTYYIQGMVMQIKGPVFVTAFNPLSMIFVAIMSS
Sbjct: 241 GNPSAWALHFDSQLLSVVYAGVICSGVTYYIQGMVMQIKGPVFVTAFNPLSMIFVAIMSS 300

Query: 301 FILSEIMLLGRIIGAVTIITGLYLVLWGKRKDQFSVKSDCDNIADSKQQMTETGEAS-KT 360
           FILSEIMLLGRI+GAVTIITGLYLVLWGKRKD+  VKSD    +DSKQQMTET E S KT
Sbjct: 301 FILSEIMLLGRIVGAVTIITGLYLVLWGKRKDRLLVKSD----SDSKQQMTETDETSKKT 360

Query: 361 VQLSQESASV 369
           VQ SQE  S+
Sbjct: 361 VQPSQEFISL 366

BLAST of Lsi04G008730 vs. ExPASy TrEMBL
Match: A0A6J1K5X5 (WAT1-related protein OS=Cucurbita maxima OX=3661 GN=LOC111490981 PE=3 SV=1)

HSP 1 Score: 555.8 bits (1431), Expect = 1.3e-154
Identity = 300/369 (81.30%), Postives = 327/369 (88.62%), Query Frame = 0

Query: 1   MEGFVKFLGLAKPYFGVLFMQFGSAGMAILAKFALNKGMSQYVFVFYRMIIATLIIAPFA 60
           MEGF++ L  AKPY GV+F+QFGSAGMAI+AK ALNKGMSQYVFVFYRM +ATL+ APFA
Sbjct: 1   MEGFMRLLVSAKPYLGVVFVQFGSAGMAIIAKSALNKGMSQYVFVFYRMAVATLVFAPFA 60

Query: 61  IIFERKVKTKMTIPLVFKIVMLGLLEPVIDLNLYFTGMKYTTATFAVAMCNVLPAFAFLM 120
           I+F+RK +TKMT+PL FKIVMLGLLEPVIDLNLYFTGMK+TTATFAVAMCNVLPAFAFLM
Sbjct: 61  IVFDRKARTKMTLPLFFKIVMLGLLEPVIDLNLYFTGMKFTTATFAVAMCNVLPAFAFLM 120

Query: 121 AWACRLEKVNILKRGSQAKILGTIVTVGGAMIMTFIRGPMLNLPWTKANHPSASST---G 180
           AWACRLEKVNILKRGSQAKI+GT+VTVGGAMIMTFI GPMLNLPWTK  HPSASS+   G
Sbjct: 121 AWACRLEKVNILKRGSQAKIIGTLVTVGGAMIMTFISGPMLNLPWTKPYHPSASSSSSAG 180

Query: 181 SVNHQSQIKGSLMIAIGCICQSAFIILQAITLKLYPAELSLTALICLVATIGGCVVALVM 240
           S NHQS IKGSLMIAIG I  SAFIILQ ITLK YPAELSLTALICLV TIGGC VALVM
Sbjct: 181 SANHQSPIKGSLMIAIGDISWSAFIILQMITLKSYPAELSLTALICLVGTIGGCGVALVM 240

Query: 241 ERGNPSAWALNFDSQLLSVVYAGVICSGVTYYIQGMVMQIKGPVFVTAFNPLSMIFVAIM 300
           ERGNPSAWA++FD QLL+VVYAGV+CSGVTYYIQG+VM+IKGPVFVTAFNPLS+I VAI+
Sbjct: 241 ERGNPSAWAVHFDRQLLAVVYAGVMCSGVTYYIQGVVMKIKGPVFVTAFNPLSLILVAIL 300

Query: 301 SSFILSEIMLLGRIIGAVTIITGLYLVLWGKRKDQFSVKSDCDNIADSKQQMTETG--EA 360
           SSFILSEIM LGRI+GAV IITGLYLVLWGK KDQ  VK + D I+  KQQMT T   E 
Sbjct: 301 SSFILSEIMFLGRIVGAVVIITGLYLVLWGKSKDQLLVKPESDKISSGKQQMTATAGEEG 360

Query: 361 SKTVQLSQE 365
           SK+VQ SQE
Sbjct: 361 SKSVQSSQE 369

BLAST of Lsi04G008730 vs. ExPASy TrEMBL
Match: A0A6J1GZ11 (WAT1-related protein OS=Cucurbita moschata OX=3662 GN=LOC111458444 PE=3 SV=1)

HSP 1 Score: 547.4 bits (1409), Expect = 4.7e-152
Identity = 297/370 (80.27%), Postives = 325/370 (87.84%), Query Frame = 0

Query: 1   MEGFVKFLGLAKPYFGVLFMQFGSAGMAILAKFALNKGMSQYVFVFYRMIIATLIIAPFA 60
           MEG ++ L  AKPY GV+F+QFGSAGMAI+AK ALNKGMSQYVFVFYRM +ATL+ APFA
Sbjct: 1   MEGVMRLLVSAKPYLGVVFVQFGSAGMAIIAKSALNKGMSQYVFVFYRMAVATLVFAPFA 60

Query: 61  IIFERKVKTKMTIPLVFKIVMLGLLEPVIDLNLYFTGMKYTTATFAVAMCNVLPAFAFLM 120
           I+F+RKV+TKMT+PL FKIVMLGLLEPVIDLNLYFTGMK+TTATFAVAMCNVLPAFAFLM
Sbjct: 61  IVFDRKVRTKMTLPLFFKIVMLGLLEPVIDLNLYFTGMKFTTATFAVAMCNVLPAFAFLM 120

Query: 121 AWACRLEKVNILKRGSQAKILGTIVTVGGAMIMTFIRGPMLNLPWTKANHPSASST---- 180
           AWACRLEKVNILKRGSQAKI+GT+VTVGGAMIMTFI GPMLNLPWTK  HPSASS+    
Sbjct: 121 AWACRLEKVNILKRGSQAKIIGTMVTVGGAMIMTFITGPMLNLPWTKPYHPSASSSSSSA 180

Query: 181 GSVNHQSQIKGSLMIAIGCICQSAFIILQAITLKLYPAELSLTALICLVATIGGCVVALV 240
           GS NHQS IKGSLMIAIG I  SAFIILQ ITLK YPAELSLTALICLV TIGGC VALV
Sbjct: 181 GSANHQSAIKGSLMIAIGDISWSAFIILQMITLKSYPAELSLTALICLVGTIGGCGVALV 240

Query: 241 MERGNPSAWALNFDSQLLSVVYAGVICSGVTYYIQGMVMQIKGPVFVTAFNPLSMIFVAI 300
           MERGNPSAWA++FD QLL+VVYAGV+CSGVTYYIQG+VM+IKGPVFVTAFNPLS+I VAI
Sbjct: 241 MERGNPSAWAVHFDRQLLAVVYAGVMCSGVTYYIQGVVMKIKGPVFVTAFNPLSLILVAI 300

Query: 301 MSSFILSEIMLLGRIIGAVTIITGLYLVLWGKRKDQFSVKSDCDNIADSKQQMTET--GE 360
           +SSFILSEIM LGRI+GAV IITGLYLVLWGK KDQ  VK + D I+  KQQM  T   E
Sbjct: 301 LSSFILSEIMFLGRIVGAVVIITGLYLVLWGKSKDQLLVKPESDKISSGKQQMIATTGEE 360

Query: 361 ASKTVQLSQE 365
            S++ Q SQE
Sbjct: 361 GSESFQSSQE 370

BLAST of Lsi04G008730 vs. NCBI nr
Match: XP_038892005.1 (WAT1-related protein At2g39510-like [Benincasa hispida])

HSP 1 Score: 644.8 bits (1662), Expect = 4.5e-181
Identity = 346/368 (94.02%), Postives = 352/368 (95.65%), Query Frame = 0

Query: 1   MEGFVKFLGLAKPYFGVLFMQFGSAGMAILAKFALNKGMSQYVFVFYRMIIATLIIAPFA 60
           MEGFV+FLGLAKPYFGVLFMQFGSAGMAILAKFALNKGMSQYVFVFYRMIIATLIIAPFA
Sbjct: 1   MEGFVRFLGLAKPYFGVLFMQFGSAGMAILAKFALNKGMSQYVFVFYRMIIATLIIAPFA 60

Query: 61  IIFERKVKTKMTIPLVFKIVMLGLLEPVIDLNLYFTGMKYTTATFAVAMCNVLPAFAFLM 120
            IF+RKV+TKMTI LVFKIVMLGLLEPVIDLNLYFTGMKYTTATFAVAMCNVLPAFAFLM
Sbjct: 61  FIFDRKVRTKMTISLVFKIVMLGLLEPVIDLNLYFTGMKYTTATFAVAMCNVLPAFAFLM 120

Query: 121 AWACRLEKVNILKRGSQAKILGTIVTVGGAMIMTFIRGPMLNLPWTKANHPSASSTGSVN 180
           AWACRLEKVNILKRGSQAKILGTIVTVGGAMIMTFIRGPMLNLPWTK NHPSASS  S N
Sbjct: 121 AWACRLEKVNILKRGSQAKILGTIVTVGGAMIMTFIRGPMLNLPWTKPNHPSASSAASTN 180

Query: 181 HQSQIKGSLMIAIGCICQSAFIILQAITLKLYPAELSLTALICLVATIGGCVVALVMERG 240
           HQSQIKGSLMIAIGCICQSAFIILQAITLKLYPAELSLTALICLVATIGGC+VALVMERG
Sbjct: 181 HQSQIKGSLMIAIGCICQSAFIILQAITLKLYPAELSLTALICLVATIGGCLVALVMERG 240

Query: 241 NPSAWALNFDSQLLSVVYAGVICSGVTYYIQGMVMQIKGPVFVTAFNPLSMIFVAIMSSF 300
           NPSAWAL+FDSQLLSVVYAGVICSGVTYYIQGMVMQIKGPVFVTAFNPLSMIFVAIMSSF
Sbjct: 241 NPSAWALHFDSQLLSVVYAGVICSGVTYYIQGMVMQIKGPVFVTAFNPLSMIFVAIMSSF 300

Query: 301 ILSEIMLLGRIIGAVTIITGLYLVLWGKRKDQFSVKSDCDNIADSKQQMTETGEASKTVQ 360
           ILSEIMLLGRIIGAVTIITGLYLVLWGKRKDQ SVKSD DNIA  KQQ TE GEA KTVQ
Sbjct: 301 ILSEIMLLGRIIGAVTIITGLYLVLWGKRKDQLSVKSDSDNIAHDKQQTTEAGEALKTVQ 360

Query: 361 LSQESASV 369
            SQE  S+
Sbjct: 361 SSQEFVSL 368

BLAST of Lsi04G008730 vs. NCBI nr
Match: XP_008461645.1 (PREDICTED: WAT1-related protein At2g39510-like [Cucumis melo])

HSP 1 Score: 612.5 bits (1578), Expect = 2.5e-171
Identity = 335/370 (90.54%), Postives = 349/370 (94.32%), Query Frame = 0

Query: 1   MEGFVKFLGLAKPYFGVLFMQFGSAGMAILAKFALNKGMSQYVFVFYRMIIATLIIAPFA 60
           MEGFV+FL LAKPYFGVLFMQ GSAGMAI+AKFALNKGMSQYVFVFYRMIIATLI+APFA
Sbjct: 1   MEGFVRFLVLAKPYFGVLFMQLGSAGMAIIAKFALNKGMSQYVFVFYRMIIATLIMAPFA 60

Query: 61  IIFERKVKTKMTIPLVFKIVMLGLLEPVIDLNLYFTGMKYTTATFAVAMCNVLPAFAFLM 120
           IIFERKV+TKMTI LVFKIVMLGLLEPVIDLNLYFTGMKYTTATFAVAMCNVLPAFAFLM
Sbjct: 61  IIFERKVRTKMTISLVFKIVMLGLLEPVIDLNLYFTGMKYTTATFAVAMCNVLPAFAFLM 120

Query: 121 AWACRLEKVNILKRGSQAKILGTIVTVGGAMIMTFIRGPMLNLPWTKANHP-SASSTGSV 180
           AWACRLEKVNI KRGSQAKI+GTIVTVGGAMIMTFIRGPMLNLPWTK NHP S+SS+ S 
Sbjct: 121 AWACRLEKVNIRKRGSQAKIVGTIVTVGGAMIMTFIRGPMLNLPWTKPNHPSSSSSSSSK 180

Query: 181 NHQSQIKGSLMIAIGCICQSAFIILQAITLKLYPAELSLTALICLVATIGGCVVALVMER 240
           NHQ QIKGSLMIAIGCICQSAFIILQAITLKLYPAELSLTALICLVAT+GGC+VALVMER
Sbjct: 181 NHQDQIKGSLMIAIGCICQSAFIILQAITLKLYPAELSLTALICLVATVGGCLVALVMER 240

Query: 241 GNPSAWALNFDSQLLSVVYAGVICSGVTYYIQGMVMQIKGPVFVTAFNPLSMIFVAIMSS 300
           GNPSAWAL+FDSQLLSVVYAGVICSGVTYYIQGMVMQIKGPVFVTAFNPLSMIFVAIMSS
Sbjct: 241 GNPSAWALHFDSQLLSVVYAGVICSGVTYYIQGMVMQIKGPVFVTAFNPLSMIFVAIMSS 300

Query: 301 FILSEIMLLGRIIGAVTIITGLYLVLWGKRKDQFSVKSDCDNIADSKQQMTETGEAS-KT 360
           FILSEIMLLGRI+GAVTIITGLYLVLWGKRKD+  VKSD    +DSKQQMTET E S KT
Sbjct: 301 FILSEIMLLGRIVGAVTIITGLYLVLWGKRKDRLLVKSD----SDSKQQMTETDETSKKT 360

Query: 361 VQLSQESASV 369
           VQ SQE  S+
Sbjct: 361 VQPSQEFISL 366

BLAST of Lsi04G008730 vs. NCBI nr
Match: XP_004147717.2 (WAT1-related protein At2g39510 [Cucumis sativus])

HSP 1 Score: 601.7 bits (1550), Expect = 4.3e-168
Identity = 327/371 (88.14%), Postives = 346/371 (93.26%), Query Frame = 0

Query: 1   MEGFVKFLGLAKPYFGVLFMQFGSAGMAILAKFALNKGMSQYVFVFYRMIIATLIIAPFA 60
           MEG  +FL LAKPYFGVLFMQ GSAGMAI+AKFALNKGMSQYVFVFYRMIIAT+I+APFA
Sbjct: 1   MEGLRRFLVLAKPYFGVLFMQLGSAGMAIIAKFALNKGMSQYVFVFYRMIIATVIMAPFA 60

Query: 61  IIFERKVKTKMTIPLVFKIVMLGLLEPVIDLNLYFTGMKYTTATFAVAMCNVLPAFAFLM 120
           IIFERKV+TKMTI L FKIVMLGLLEPVIDLNLYFTGMKYTTATFAVAMCNVLPAFAFLM
Sbjct: 61  IIFERKVRTKMTISLAFKIVMLGLLEPVIDLNLYFTGMKYTTATFAVAMCNVLPAFAFLM 120

Query: 121 AWACRLEKVNILKRGSQAKILGTIVTVGGAMIMTFIRGPMLNLPWTKANHP--SASSTGS 180
           AWACRLE+VNI KRGSQAKI+GTIVT+GGAMIMTFIRGPMLNLPWTK NHP  S+SS+ S
Sbjct: 121 AWACRLERVNIWKRGSQAKIVGTIVTIGGAMIMTFIRGPMLNLPWTKPNHPSSSSSSSSS 180

Query: 181 VNHQSQIKGSLMIAIGCICQSAFIILQAITLKLYPAELSLTALICLVATIGGCVVALVME 240
           +NH +QIKGSLMI IGCICQSAFIILQAITLKLYPAELSLTALICLVAT+GGC+VALVME
Sbjct: 181 INHHNQIKGSLMITIGCICQSAFIILQAITLKLYPAELSLTALICLVATVGGCLVALVME 240

Query: 241 RGNPSAWALNFDSQLLSVVYAGVICSGVTYYIQGMVMQIKGPVFVTAFNPLSMIFVAIMS 300
           RGNPSAWAL+FDSQLLSVVYAGVICSGVTYYIQGMVMQIKGPVFVTAFNPLSMIFVAIMS
Sbjct: 241 RGNPSAWALHFDSQLLSVVYAGVICSGVTYYIQGMVMQIKGPVFVTAFNPLSMIFVAIMS 300

Query: 301 SFILSEIMLLGRIIGAVTIITGLYLVLWGKRKDQFSVKSDCDNIADSKQQMTETGEASK- 360
           SFILSEIMLLGRI+GAVTIITGLYLVLWGKRKD+  VKSD    +D KQQMTET EASK 
Sbjct: 301 SFILSEIMLLGRIVGAVTIITGLYLVLWGKRKDKLLVKSD----SDGKQQMTETDEASKR 360

Query: 361 TVQLSQESASV 369
           TVQ SQE  S+
Sbjct: 361 TVQPSQEFISL 367

BLAST of Lsi04G008730 vs. NCBI nr
Match: KAA0050295.1 (WAT1-related protein [Cucumis melo var. makuwa])

HSP 1 Score: 573.2 bits (1476), Expect = 1.7e-159
Identity = 311/370 (84.05%), Postives = 335/370 (90.54%), Query Frame = 0

Query: 1   MEGFVKFLGLAKPYFGVLFMQFGSAGMAILAKFALNKGMSQYVFVFYRMIIATLIIAPFA 60
           ME FV+F+  AKPYFGV+F+QFG AGM IL K AL+KGMSQ+VFV YR + ATL+IAPFA
Sbjct: 1   MESFVRFMRSAKPYFGVIFVQFGYAGMTILTKSALDKGMSQHVFVAYRQVAATLVIAPFA 60

Query: 61  IIFERKVKTKMTIPLVFKIVMLGLLEPVIDLNLYFTGMKYTTATFAVAMCNVLPAFAFLM 120
           IIFERK +TKMT  L+FKI++LG LEPVID NL++TGMKYTTATFA AMCNVLPAF FLM
Sbjct: 61  IIFERKARTKMTFSLLFKILLLGFLEPVIDQNLFYTGMKYTTATFAAAMCNVLPAFVFLM 120

Query: 121 AWACRLEKVNILKRGSQAKILGTIVTVGGAMIMTFIRGPMLNLPWTKANHP-SASSTGSV 180
           AWACRLEKVNI KRGSQAKI+GTIVTVGGAMIMTFIRGPMLNLPWTK NHP S+SS+ S 
Sbjct: 121 AWACRLEKVNIWKRGSQAKIVGTIVTVGGAMIMTFIRGPMLNLPWTKPNHPSSSSSSSSK 180

Query: 181 NHQSQIKGSLMIAIGCICQSAFIILQAITLKLYPAELSLTALICLVATIGGCVVALVMER 240
           NHQ QIKGSLMIAIGCICQSAFIILQAITLKLYPAELSLTALICLVAT+GGC+VALVMER
Sbjct: 181 NHQDQIKGSLMIAIGCICQSAFIILQAITLKLYPAELSLTALICLVATVGGCLVALVMER 240

Query: 241 GNPSAWALNFDSQLLSVVYAGVICSGVTYYIQGMVMQIKGPVFVTAFNPLSMIFVAIMSS 300
           GNPSAWAL+FDSQLLSVVYAGVICSGVTYYIQGMVMQIKGPVFVTAFNPLSMIFVAIMSS
Sbjct: 241 GNPSAWALHFDSQLLSVVYAGVICSGVTYYIQGMVMQIKGPVFVTAFNPLSMIFVAIMSS 300

Query: 301 FILSEIMLLGRIIGAVTIITGLYLVLWGKRKDQFSVKSDCDNIADSKQQMTETGEAS-KT 360
           FILSEIMLLGRI+GAVTIITGLYLVLWGKRKD+  VKSD    +DSKQQMTET E S KT
Sbjct: 301 FILSEIMLLGRIVGAVTIITGLYLVLWGKRKDRLLVKSD----SDSKQQMTETDETSKKT 360

Query: 361 VQLSQESASV 369
           VQ SQE  S+
Sbjct: 361 VQPSQEFISL 366

BLAST of Lsi04G008730 vs. NCBI nr
Match: XP_022995484.1 (WAT1-related protein At2g39510-like [Cucurbita maxima])

HSP 1 Score: 555.8 bits (1431), Expect = 2.7e-154
Identity = 300/369 (81.30%), Postives = 327/369 (88.62%), Query Frame = 0

Query: 1   MEGFVKFLGLAKPYFGVLFMQFGSAGMAILAKFALNKGMSQYVFVFYRMIIATLIIAPFA 60
           MEGF++ L  AKPY GV+F+QFGSAGMAI+AK ALNKGMSQYVFVFYRM +ATL+ APFA
Sbjct: 1   MEGFMRLLVSAKPYLGVVFVQFGSAGMAIIAKSALNKGMSQYVFVFYRMAVATLVFAPFA 60

Query: 61  IIFERKVKTKMTIPLVFKIVMLGLLEPVIDLNLYFTGMKYTTATFAVAMCNVLPAFAFLM 120
           I+F+RK +TKMT+PL FKIVMLGLLEPVIDLNLYFTGMK+TTATFAVAMCNVLPAFAFLM
Sbjct: 61  IVFDRKARTKMTLPLFFKIVMLGLLEPVIDLNLYFTGMKFTTATFAVAMCNVLPAFAFLM 120

Query: 121 AWACRLEKVNILKRGSQAKILGTIVTVGGAMIMTFIRGPMLNLPWTKANHPSASST---G 180
           AWACRLEKVNILKRGSQAKI+GT+VTVGGAMIMTFI GPMLNLPWTK  HPSASS+   G
Sbjct: 121 AWACRLEKVNILKRGSQAKIIGTLVTVGGAMIMTFISGPMLNLPWTKPYHPSASSSSSAG 180

Query: 181 SVNHQSQIKGSLMIAIGCICQSAFIILQAITLKLYPAELSLTALICLVATIGGCVVALVM 240
           S NHQS IKGSLMIAIG I  SAFIILQ ITLK YPAELSLTALICLV TIGGC VALVM
Sbjct: 181 SANHQSPIKGSLMIAIGDISWSAFIILQMITLKSYPAELSLTALICLVGTIGGCGVALVM 240

Query: 241 ERGNPSAWALNFDSQLLSVVYAGVICSGVTYYIQGMVMQIKGPVFVTAFNPLSMIFVAIM 300
           ERGNPSAWA++FD QLL+VVYAGV+CSGVTYYIQG+VM+IKGPVFVTAFNPLS+I VAI+
Sbjct: 241 ERGNPSAWAVHFDRQLLAVVYAGVMCSGVTYYIQGVVMKIKGPVFVTAFNPLSLILVAIL 300

Query: 301 SSFILSEIMLLGRIIGAVTIITGLYLVLWGKRKDQFSVKSDCDNIADSKQQMTETG--EA 360
           SSFILSEIM LGRI+GAV IITGLYLVLWGK KDQ  VK + D I+  KQQMT T   E 
Sbjct: 301 SSFILSEIMFLGRIVGAVVIITGLYLVLWGKSKDQLLVKPESDKISSGKQQMTATAGEEG 360

Query: 361 SKTVQLSQE 365
           SK+VQ SQE
Sbjct: 361 SKSVQSSQE 369

BLAST of Lsi04G008730 vs. TAIR 10
Match: AT2G39510.1 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 389.0 bits (998), Expect = 4.1e-108
Identity = 205/332 (61.75%), Postives = 259/332 (78.01%), Query Frame = 0

Query: 12  KPYFGVLFMQFGSAGMAILAKFALNKGMSQYVFVFYRMIIATLIIAPFAIIFERKVKTKM 71
           KP+  V+ +QFG AG++I+AKFALN+GMS +V   YR I+AT+ IAPFA   +RK++ KM
Sbjct: 7   KPFITVVSLQFGYAGLSIIAKFALNQGMSPHVLASYRHIVATIFIAPFAYFLDRKIRPKM 66

Query: 72  TIPLVFKIVMLGLLEPVIDLNLYFTGMKYTTATFAVAMCNVLPAFAFLMAWACRLEKVNI 131
           T+ + FKI++LGLLEP ID NLY+TGMKYT+ATF  AM NVLPAFAF+MAW  RLEKVN+
Sbjct: 67  TLSIFFKILLLGLLEPTIDQNLYYTGMKYTSATFTAAMTNVLPAFAFIMAWIFRLEKVNV 126

Query: 132 LKRGSQAKILGTIVTVGGAMIMTFIRGPMLNLPWTKAN--HPSASSTGSVNHQSQIKGSL 191
            K  SQAKILGTIVTVGGAM+MT ++GP++ LPW   +  H  +S+TG    Q   KG+ 
Sbjct: 127 KKIHSQAKILGTIVTVGGAMLMTVVKGPLIPLPWANPHDIHQDSSNTGV--KQDLTKGAS 186

Query: 192 MIAIGCICQSAFIILQAITLKLYPAELSLTALICLVATIGGCVVALVMERGNPSAWALNF 251
           +IAIGCIC + FI LQAITLK YP ELSLTA IC + +I   +VAL +ERGNPSAWA++ 
Sbjct: 187 LIAIGCICWAGFINLQAITLKSYPVELSLTAYICFLGSIESTIVALFIERGNPSAWAIHL 246

Query: 252 DSQLLSVVYAGVICSGVTYYIQGMVMQIKGPVFVTAFNPLSMIFVAIMSSFILSEIMLLG 311
           DS+LL+ VY GVICSG+ YY+QG++M+ +GPVFVTAFNPLSM+ VAI+ S IL+E+M LG
Sbjct: 247 DSKLLAAVYGGVICSGIGYYVQGVIMKTRGPVFVTAFNPLSMVIVAILGSIILAEVMFLG 306

Query: 312 RIIGAVTIITGLYLVLWGKRKDQFSVK-SDCD 341
           RI+GA+ I+ GLY VLWGK KD+ S   SD D
Sbjct: 307 RILGAIVIVLGLYSVLWGKSKDEPSSSFSDMD 336

BLAST of Lsi04G008730 vs. TAIR 10
Match: AT2G37460.1 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 353.6 bits (906), Expect = 1.9e-97
Identity = 185/321 (57.63%), Postives = 244/321 (76.01%), Query Frame = 0

Query: 11  AKPYFGVLFMQFGSAGMAILAKFALNKGMSQYVFVFYRMIIATLIIAPFAIIFERKVKTK 70
           A+P+  ++ +Q G AGM IL+K  LNKGMS YV V YR  +AT+++APFA  F++KV+ K
Sbjct: 13  ARPFISMVVLQVGLAGMDILSKAVLNKGMSNYVLVVYRHAVATIVMAPFAFYFDKKVRPK 72

Query: 71  MTIPLVFKIVMLGLLEPVIDLNLYFTGMKYTTATFAVAMCNVLPAFAFLMAWACRLEKVN 130
           MT+ + FKI +LGLLEPVID NLY+ GMKYTTATFA AM NVLPA  F++A+   LE+V 
Sbjct: 73  MTLMIFFKISLLGLLEPVIDQNLYYLGMKYTTATFATAMYNVLPAITFVLAYIFGLERVK 132

Query: 131 ILKRGSQAKILGTIVTVGGAMIMTFIRGPMLNLPWTKANHPSASSTGSVNHQSQIKGSLM 190
           +    S  K++GT+ TVGGAMIMT ++GP+L+L WTK    SA +T   +  S IKG+++
Sbjct: 133 LRCIRSTGKVVGTLATVGGAMIMTLVKGPVLDLFWTKG--VSAHNTAGTDIHSAIKGAVL 192

Query: 191 IAIGCICQSAFIILQAITLKLYPAELSLTALICLVATIGGCVVALVMERGNPSAWALNFD 250
           + IGC   + F+ILQAITL+ YPAELSLTA ICL+ TI G  VALVME+GNPSAWA+ +D
Sbjct: 193 VTIGCFSYACFMILQAITLRTYPAELSLTAWICLMGTIEGTAVALVMEKGNPSAWAIGWD 252

Query: 251 SQLLSVVYAGVICSGVTYYIQGMVMQIKGPVFVTAFNPLSMIFVAIMSSFILSEIMLLGR 310
           ++LL+  Y+G++CS + YY+ G+VM+ +GPVFVTAF+PL MI VAIMS+ I +E M LGR
Sbjct: 253 TKLLTATYSGIVCSALAYYVGGVVMKTRGPVFVTAFSPLCMIIVAIMSTIIFAEQMYLGR 312

Query: 311 IIGAVTIITGLYLVLWGKRKD 332
           ++GAV I  GLYLV+WGK KD
Sbjct: 313 VLGAVVICAGLYLVIWGKGKD 331

BLAST of Lsi04G008730 vs. TAIR 10
Match: AT4G08290.1 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 316.6 bits (810), Expect = 2.6e-86
Identity = 161/333 (48.35%), Postives = 240/333 (72.07%), Query Frame = 0

Query: 1   MEGFVKFLGLAKPYFGVLFMQFGSAGMAILAKFALNKGMSQYVFVFYRMIIATLIIAPFA 60
           MEG    +   +PY  ++F+QFG+AG  I+    LN+G ++YV + YR ++A L++APFA
Sbjct: 1   MEGVSATMHKLRPYLLMIFLQFGAAGTYIVIMATLNQGQNRYVVIVYRNLVAALVLAPFA 60

Query: 61  IIFERKVKTKMTIPLVFKIVMLGLLEPVIDLNLYFTGMKYTTATFAVAMCNVLPAFAFLM 120
           +IFERKV+ KMT+ +++KI+ LG LEPV+D    + GM  T+AT+  A+ N+LP+  F++
Sbjct: 61  LIFERKVRPKMTLSVLWKIMALGFLEPVLDQGFGYLGMNMTSATYTSAIMNILPSVTFII 120

Query: 121 AWACRLEKVNILKRGSQAKILGTIVTVGGAMIMTFIRGPMLNLPWTKAN--HPSASSTGS 180
           AW  R+EKVNI +  S+AKI+GT+V +GGA++MT  +GP++ LPW+  N    +  +  S
Sbjct: 121 AWILRMEKVNIAEVRSKAKIIGTLVGLGGALVMTLYKGPLIPLPWSNPNMDQQNGHTNNS 180

Query: 181 VNHQSQIKGSLMIAIGCICQSAFIILQAITLKLYPAELSLTALICLVATIGGCVVALVME 240
            +H + + G+L+I +GC+  S F +LQ+IT+K YPA+LSL+ALICL   +    VALV+E
Sbjct: 181 QDHNNWVVGTLLILLGCVAWSGFYVLQSITIKTYPADLSLSALICLAGAVQSFAVALVVE 240

Query: 241 RGNPSAWALNFDSQLLSVVYAGVICSGVTYYIQGMVMQIKGPVFVTAFNPLSMIFVAIMS 300
           R +PS WA+ +D++L + +Y G++ SG+TYY+QGMVM+ +GPVFVTAFNPL MI VA+++
Sbjct: 241 R-HPSGWAVGWDARLFAPLYTGIVSSGITYYVQGMVMKTRGPVFVTAFNPLCMILVALIA 300

Query: 301 SFILSEIMLLGRIIGAVTIITGLYLVLWGKRKD 332
           SFIL E +  G +IG   I  GLY+V+WGK KD
Sbjct: 301 SFILHEQIHFGCVIGGAVIAAGLYMVVWGKGKD 332

BLAST of Lsi04G008730 vs. TAIR 10
Match: AT2G37450.2 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 301.2 bits (770), Expect = 1.1e-81
Identity = 168/321 (52.34%), Postives = 214/321 (66.67%), Query Frame = 0

Query: 11  AKPYFGVLFMQFGSAGMAILAKFALNKGMSQYVFVFYRMIIATLIIAPFAIIFERKVKTK 70
           A P+  ++ +Q G AGM IL K  LNKGMS YV   YR  +AT+++APFA  F+      
Sbjct: 7   ALPFILMVLLQIGYAGMDILTKDVLNKGMSIYVLSVYRHGVATVVMAPFAFYFD------ 66

Query: 71  MTIPLVFKIVMLGLLEPVIDLNLYFTGMKYTTATFAVAMCNVLPAFAFLMAWACRLEKVN 130
                           PVI  NL+  GMKYTTATFA+A+ N LPA  F++A   RLE V 
Sbjct: 67  ---------------NPVIAQNLFNLGMKYTTATFAIALYNTLPAVTFILALIFRLESVK 126

Query: 131 ILKRGSQAKILGTIVTVGGAMIMTFIRGPMLNLPWTKANHPSASSTGSVNHQSQIKGSLM 190
                S AK++GT+ TVGG M+MT ++GP L+L WTK   PSA +T   +  S IKG+++
Sbjct: 127 FQSIRSAAKVVGTVTTVGGIMVMTLVKGPALDLFWTKG--PSAQNTVGTDIHSSIKGAVL 186

Query: 191 IAIGCICQSAFIILQAITLKLYPAELSLTALICLVATIGGCVVALVMERGNPSAWALNFD 250
           + IGC   + F+ILQAITLK YPAELSL   ICL+ TI G VVALVME+GNPS WA+ +D
Sbjct: 187 VTIGCFSYACFMILQAITLKTYPAELSLATWICLIGTIEGVVVALVMEKGNPSVWAIGWD 246

Query: 251 SQLLSVVYAGVICSGVTYYIQGMVMQIKGPVFVTAFNPLSMIFVAIMSSFILSEIMLLGR 310
           ++LL++ Y+G++CS + YYI G+VM+ +GPVFVTAF PL MI VAIMSS I  E M LGR
Sbjct: 247 TKLLTITYSGIVCSALGYYIGGVVMKTRGPVFVTAFKPLCMIVVAIMSSIIFDEQMYLGR 304

Query: 311 IIGAVTIITGLYLVLWGKRKD 332
            +GA  I  GLYLV+WGK KD
Sbjct: 307 ALGATVICVGLYLVIWGKAKD 304

BLAST of Lsi04G008730 vs. TAIR 10
Match: AT5G07050.1 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 297.4 bits (760), Expect = 1.6e-80
Identity = 169/358 (47.21%), Postives = 237/358 (66.20%), Query Frame = 0

Query: 7   FLGLAKPYFGVLFMQFGSAGMAILAKFALNKGMSQYVFVFYRMIIATLIIAPFAIIFERK 66
           FL  +KPYF ++ +QFG AGM I+ K +LN GMS YV V YR  IAT +IAPFA  FERK
Sbjct: 12  FLTSSKPYFAMISLQFGYAGMNIITKISLNTGMSHYVLVVYRHAIATAVIAPFAFFFERK 71

Query: 67  VKTKMTIPLVFKIVMLGLLEPVIDLNLYFTGMKYTTATFAVAMCNVLPAFAFLMAWACRL 126
            + K+T  +  ++ +LGLL PVID N Y+ G+KYT+ TF+ AM N+LPA  F++A   R+
Sbjct: 72  AQPKITFSIFMQLFILGLLGPVIDQNFYYMGLKYTSPTFSCAMSNMLPAMTFILAVLFRM 131

Query: 127 EKVNILKRGSQAKILGTIVTVGGAMIMTFIRGPMLNLPWTKANH----PSASSTGSVNHQ 186
           E +++ K   QAKI GT+VTV GAM+MT  +GP++ L WTK  H      A++T S N  
Sbjct: 132 EMLDLKKLWCQAKIAGTVVTVAGAMLMTIYKGPIVELFWTKYMHIQDSSHANTTSSKNSS 191

Query: 187 SQ---IKGSLMIAIGCICQSAFIILQAITLKLYPA-ELSLTALICLVATIGGCVVALVME 246
           S    +KGS+++    +  ++  +LQA  LK Y   +LSLT LIC + T+    V  VME
Sbjct: 192 SDKEFLKGSILLIFATLAWASLFVLQAKILKTYAKHQLSLTTLICFIGTLQAVAVTFVME 251

Query: 247 RGNPSAWALNFDSQLLSVVYAGVICSGVTYYIQGMVMQIKGPVFVTAFNPLSMIFVAIMS 306
             NPSAW + +D  LL+  Y+G++ S ++YY+QG+VM+ +GPVF TAF+PL M+ VA+M 
Sbjct: 252 H-NPSAWRIGWDMNLLAAAYSGIVASSISYYVQGIVMKKRGPVFATAFSPLMMVIVAVMG 311

Query: 307 SFILSEIMLLGRIIGAVTIITGLYLVLWGKRKDQFSVKSDCDNIADSKQQMTETGEAS 357
           SF+L+E + LG +IGAV I+ GLY VLWGK+K+      +   I DS  ++TE  EA+
Sbjct: 312 SFVLAEKIFLGGVIGAVLIVIGLYAVLWGKQKENQVTICELAKI-DSNSKVTEDVEAN 367

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O806385.8e-10761.75WAT1-related protein At2g39510 OS=Arabidopsis thaliana OX=3702 GN=At2g39510 PE=2... [more]
Q9ZUS12.7e-9657.63WAT1-related protein At2g37460 OS=Arabidopsis thaliana OX=3702 GN=At2g37460 PE=2... [more]
Q9SUF13.7e-8548.35WAT1-related protein At4g08290 OS=Arabidopsis thaliana OX=3702 GN=At4g08290 PE=2... [more]
F4IQX11.6e-8052.34WAT1-related protein At2g37450 OS=Arabidopsis thaliana OX=3702 GN=At2g37450 PE=2... [more]
Q9FL412.3e-7947.21WAT1-related protein At5g07050 OS=Arabidopsis thaliana OX=3702 GN=At5g07050 PE=2... [more]
Match NameE-valueIdentityDescription
A0A1S3CF331.2e-17190.54WAT1-related protein OS=Cucumis melo OX=3656 GN=LOC103500199 PE=3 SV=1[more]
A0A0A0KS682.1e-16888.14WAT1-related protein OS=Cucumis sativus OX=3659 GN=Csa_5G181430 PE=3 SV=1[more]
A0A5A7U9L78.0e-16084.05WAT1-related protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold88G... [more]
A0A6J1K5X51.3e-15481.30WAT1-related protein OS=Cucurbita maxima OX=3661 GN=LOC111490981 PE=3 SV=1[more]
A0A6J1GZ114.7e-15280.27WAT1-related protein OS=Cucurbita moschata OX=3662 GN=LOC111458444 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_038892005.14.5e-18194.02WAT1-related protein At2g39510-like [Benincasa hispida][more]
XP_008461645.12.5e-17190.54PREDICTED: WAT1-related protein At2g39510-like [Cucumis melo][more]
XP_004147717.24.3e-16888.14WAT1-related protein At2g39510 [Cucumis sativus][more]
KAA0050295.11.7e-15984.05WAT1-related protein [Cucumis melo var. makuwa][more]
XP_022995484.12.7e-15481.30WAT1-related protein At2g39510-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
AT2G39510.14.1e-10861.75nodulin MtN21 /EamA-like transporter family protein [more]
AT2G37460.11.9e-9757.63nodulin MtN21 /EamA-like transporter family protein [more]
AT4G08290.12.6e-8648.35nodulin MtN21 /EamA-like transporter family protein [more]
AT2G37450.21.1e-8152.34nodulin MtN21 /EamA-like transporter family protein [more]
AT5G07050.11.6e-8047.21nodulin MtN21 /EamA-like transporter family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000620EamA domainPFAMPF00892EamAcoord: 14..153
e-value: 2.0E-12
score: 47.4
coord: 186..325
e-value: 6.5E-10
score: 39.3
NoneNo IPR availablePANTHERPTHR31218:SF215WAT1-RELATED PROTEINcoord: 9..341
NoneNo IPR availableSUPERFAMILY103481Multidrug resistance efflux transporter EmrEcoord: 231..330
NoneNo IPR availableSUPERFAMILY103481Multidrug resistance efflux transporter EmrEcoord: 47..155
IPR030184WAT1-related proteinPANTHERPTHR31218WAT1-RELATED PROTEINcoord: 9..341

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi04G008730.1Lsi04G008730.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005886 plasma membrane
cellular_component GO:0016020 membrane
molecular_function GO:0022857 transmembrane transporter activity