Lsi04G008290 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi04G008290
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionGlutathione S-transferase family protein
Locationchr04: 8002650 .. 8008050 (-)
RNA-Seq ExpressionLsi04G008290
SyntenyLsi04G008290
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
CGAAGTTTAGCTGAGTTGTAATAGAAAAGAAAAAGAAAAAGAAAAAACGAAGCAATTTCATTTGATAATCATGGCGACTTCGACACTGGGCAGCACCATAGGCTTCTCCCTGCCGTCGTCTTCCTCAGGTACCTCAGCTTATTGTACTTCCTCTTCAACCAGCAGGTTCAGCTTCTCGAGCACCATTCTTTCTCCTCTTAAGCTTAAAAAACAATCATCTGGAAGAAGAGCCAGAGCTGTAATTGCTGTGGAAATGGCTGCTGAGTATGTTCACTTCTTCCCTTCTTTCTTTGCTGTATGAGAAAATGGAGGGGTATGTACTACTTTGAAATTCTTTCTCTTCGTTCTCAAGAGGAAATAGCGAGTTTAAAGTTTAGGTTATTTCTTTTTTCTCATGCTATCGATCCGAATGGGGTTTCTTTCAAATTTACATTTCTTCCGAGTATAGATGCAGGGTCATTGAATGTTAGAAGGTAGAAGATTTGATGGAATCAAAGATTAGGATTCTGTTACAAAATTGATACAAGTACAATTTTGAGTTCGATTATTGTGTTGACGGATTATAGAAGAAGTTGCAGATAGCTTTTGCGAAATTACATTCGTTTGTAGTTTGATATTTCAATGTTCTTACTGATTTATTGTTTATGTCGATGTCTTGAAACTTAGGAATAAGAGAAGCAATTAAGGTTGAATTTATATTAGTGATTTGTTGTAAAGTTTCTGTCAGTATTTTCACCTTTTGTTTTGTGTGGGAAAGAGCTTTTCAGGAAGTGCTTCCTCCAGCCCTCACTTCATCTTCAGAACCACCTCCTATCTTTGATGGAACAACGAGGTTGGTTCAATTTTCATCCATCCACTGTAGTTCATGCTCCCACTAAACAAGACACTCAAGTTGTTAGACCATGGCATTTATTTTTTCTTTAGATATAATCTTTTAACACTTGCTTGAAGGTTAATCTAATATGTGATTCTTAACAATTAAACGTTAACATCAATTGGGGATGAAAAGACATTGTAGTGCTTCAAACTCTTGAGATCCTGTCCTGATATCATATAACATGTTTAAGCATCAACAAATCTAAGAACATAAGTTAATACATTTATATCCTTAACATTCATTTTATCATATGAAAATTCCTTGTTATATATACGAATGGCATGCCAATTTGATGCTTCGTTTGAATGCCTATGTGATGCTGACAATGCTGCTGTTTCGCTTCAACAATTGTAGGCTTTACATATCTTATACATGCCCTTATGCGCAGCGTGTGTGGATTACTCGGAACTGTAAGGTTGTTTCTCTCACTCCCCAAATAACTTCCACTGATTAAGCATGTTTGGTTGTTTTTTCATGTCAGGTTTGTTTCTTTTGCCGCATTTTGAAGATCAACATTGCTCTCCTTGACATTAATTATAAGTTCATACTATTGTTTTGGAGCAGGGTTTGCAGAATAAGATACAATTGGTTCCAATAAATCTACAAGATAGGCCTTCTTGGTACAAGGAAAAACTTTACCCACCTAACAAGGTAAAGATTTTCAGTACATTATACCTATGAATTATAATCATGGTAGTCAAATAACAACGATTACGTTTTTTATTTGCCCGTGAAGAAGAAAGAATTTTGATGCCTAGTTCTGTCACTTTGATGGTGGTACAGGTGAAGTTAGAAATTTTATCGAAGGGGCAAACTCTTACATTCAAACTTATATATTTGCATAGTTGGGGATATTAGATAGAGCTTCTAAATTATAATTCTTTTATATCTATTCTTGCATCTAAAATAAACTTAGGAAGCAACATCTCCCTCCCTTCTTCTTTGCTCCTCTTGCTCTAGTGAGGTTTTCAATATTAGGAAAAATTGAGAGAGAATTGAGTGACAAAAAACTTTAGCTGAAAATAAGAAACGAATAGAAAGTGGTCACTGAACTAACCTCTGCACTCGATGTGAAAAGATGGCCATTGTTAAATACAATTAAGCTGCTGTCTATTTCCTCTTTGATGTTTTTGACTTCTGAGCACTGGCGCAGGTGCCAGCTCTTGAACATAATAATGAAGTCAAAGGAGAGAGTCTTGACTTAATTAAATATATTGACAGCAATTTTGAAGGACCTTCATTATTTCCTGATGTAAGTATCCAGGAAAGCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCGTTTCTAATGCCTCACCTGCTTGAATTAAATGTCCATTAGCACTGAAGCATCCTAAATCTGAATATATAGGATTCAGAAAAGAGAGAGATTGCTGAAGAGTTGATAAACTACGTGAATTCTTTCACTGGATCTGTGGTTTCTTCATTTAAAGGAGATGGAAATGAAGCTGGTAAGATATTAGCAGCTTCTTCTTCCTCTTCCATGTTTTTTTACTGGAAAACACAGTTGAATCATTCAAATGATAAATCTTTTCTCAGATGTGGCGTTTGACCACATCGAATCCGCTCTTTCAAAATATGGAGATGGCCCATTTTTTCTAGGCCAGTTTAGTCTGGTAAGTAGCAACTTTCACTTTTTATGGTAGGTCAGACTTTACATCATTTATTGCTGAGGTTGATGTTGATGGAAATTTTATAGATTTGTCAATATTGATAAAATGTTAATATCGATGGATATTTCTATAAAAGCCATGGTAGCTTAAAAAAAACTCATAAAAATCATTTAAATGAATTATAAATATTTTAAGCTAAAAATCTATATTACTATTTATGTTAGATGTTAGTTTTTTTTTTAATTTTGTTGATATTTTTACCATATACCTTAAAATTATGTGAAAAAAATCAATTTATACCTCTAAATTTTGAGGGTTGTCTCAATTAAAATGTTTTAAATTTAGGTTGCCCCTTGCGAAGGGTAACAAGCTCACGCTACCATCGGCATCTCAATTAAAATGTTAAACTAATAATTGTATAAGTTTCAACCATAAATTTTTATAAGTGGTTCAATTTATATTCTCGTTAATTATTTGTAACTATTTTTCCTTACTTCCATTTAAAATCCAACAAGTATTCTTTTTCCAAAAGCCTACCATTGTTTCCTAAATAAGTCTGAGAAAATTAGAGGAAGACTGAAGGATGAAAAACTACTTATATTAATGAAAAACTTCTATCATCCATTTAGTTTCAGCATTTCTTTCTAATATTAAGTAAATGCAGTCTTATACACATGTGAAAATCATGCATTAATAGTTTTAAAACGGAACCTTTACAAAAAGTTTAAATTCACACATTTATAAAAATTTAGAAGTTTGTTTGATACAATTATTAGTTTAGAATTTTAGGTGAGACAAGTATCAAAGTTTAGAGGTATAAATTGATTTTCTCAAGTTAATGTAAAACCCTTCCATTAACGAAAACATCGATGAAATACCATAAATCATATATTGTATAATTTTTTTTCGCTCTCAGGTGGATATAGCTTATGCTCCGTTTATTGAAAGGTTTCAGCCGTTCTTATTGGAAGTAAAAACATATGACATCACTGCTGGCAGGCCAAAACTAGCAGCTTGGATTGAGGTACTGAAAATATCTCTTTCTGATCTTTAAGAAATTGTTGCGTTCGCTCTTCATCTATTATAAGACTAGTGAGTGATGGAACCAAAAGGGGGATGCCCATGAATCATGATTTTGATATATTGTGCAGTAAATACCATTTTGGTCCCCTGCCAACAACATGAAGTAGTTGTAAGCAAGGTTTAAAATATCGATATCGATGAAAATATCGAGATCTTGATTTTACAGAAATTTCGATGGAAATATCGATAAAATATTGATTTCGATGAATATTTCTGGAAAAATTTTAAAAACAAAAAAATTTAAAAATAAATTTAAATAAGTAAATAAACATTTTATTATTTCCAAATAAGTAAACATGTCTATTGTATCTATTATATTTATATTAGTGGTATTTTGATATATTTTTTTTGTGTTTTGTAGATTTCTCTACGATATAATAGAAATATCGATTCACCCCTCATATCAATATCAAACTCATGGAAACATGGAAATATCGACATATCGATGAAAATTTAATACTATGGTTGTAAGATTAGTTGTAAGATTTATTGAAAGTACAAGGACTAAAATTTGACATGTGAAAAGGAACTAAAATAGAATGAGACCCAAAATGGTATTTTAACCTTGTGATATATTAATCTCTACTAGTTGTAGTAAAATGAATAATATCCGAACAACTTTTTCTTTGAATTAGACCAGACTAACAACTTTTTCTTTGAATTAGACCAGACTAAAAGAAGGCAAAGATCATGGCAATTGGACACTGGGGAGGGATGTAAGTGACTCGGGTCAGTTTTTGGTCAACACCGAGACCAAAATTAATTATTTTAAAATCTGAATAATTCACCTAACCAATACTAAATCCAAAATGAATCAATTTGGCAATTTTGTCACTTCTTTTTGTTGAAGGTGTTTGACTTTCCATTCTATTTGGCACACCAAGCTCTAAATTGATATCTACATATGGGGTGTCACAGAGGTTAATTGTGTGCCTTGAGTCTGTTGTCGTAAACAGGGTAAGTGGGTAACCTTCTATAATATTTGCTCATTTTCTATTGCTTAGGAGATGAACAAGATCGAGGGGTACCGACAAACAAGGGGCGATCCACAGGAACATGTTGATAGCTTTAAGAAGCGATTCTTGGTAATTCTCTCTGATATTCCAAGATTATTATTATTTTTGGATATCTATAAATGTTAGGACCAACTTGCACGCACCTTAACTATCGAATATCTATAAAGGTTAAGGCCATCTTGTATGCACATTTGTTATATGTAAAGGTAGTCGTAGATATTTAATGTAAGAGGTTACCAAGGCATTTTTTTCCTTAACCACTCAAGTAATTCTCTGACGTAAAATTTTGAATGGTAATTGTTAGAGTATTGATAGAATGATAAATTTATGTTCACCCACCTACTTAAACTTTTGAGTCAATCGGTAATTTAACATTAACCTATGTTTTTGGTTAAATTTGATTGTGGAAGTCAAGATCGGAGAAACATCACACTATTTAGATCCCCATCAGTGTATGGATGAAATTTTTCTAAAGCTAATCTGTCACAACAATTATTGAAAAATTGAATCATGAGAATGAAAAGAAAACAAGTTTAGATAAATCTCAATGACTTGGTTGACATTATTATATCATTTATTCTTTTATCCTGTGCAGAGCCGTTTGTGAAAGCCACATCAGCATAGGGTCCCCATGGCAATGGTTAACTGAACACGTGCTGTCACGTGTGGATGTTGCTTCTTGTAATGAGATAACTTCATGTGTAACATTTAACAAATAAAGGAGCGTTTGAGATCATTGTACCTTTTGTTTTTTTTTTTTTTTTCAGATGGATTATTGA

mRNA sequence

CGAAGTTTAGCTGAGTTGTAATAGAAAAGAAAAAGAAAAAGAAAAAACGAAGCAATTTCATTTGATAATCATGGCGACTTCGACACTGGGCAGCACCATAGGCTTCTCCCTGCCGTCGTCTTCCTCAGGTACCTCAGCTTATTGTACTTCCTCTTCAACCAGCAGGTTCAGCTTCTCGAGCACCATTCTTTCTCCTCTTAAGCTTAAAAAACAATCATCTGGAAGAAGAGCCAGAGCTGTAATTGCTGTGGAAATGGCTGCTGAGTATGTTCACTTCTTCCCTTCTTTCTTTGCTGTATGAGAAAATGGAGGGTATTTTCACCTTTTGTTTTGTGTGGGAAAGAGCTTTTCAGGAAGTGCTTCCTCCAGCCCTCACTTCATCTTCAGAACCACCTCCTATCTTTGATGGAACAACGAGGCTTTACATATCTTATACATGCCCTTATGCGCAGCGTGTGTGGATTACTCGGAACTGTAAGGGTTTGCAGAATAAGATACAATTGGTTCCAATAAATCTACAAGATAGGCCTTCTTGGTACAAGGAAAAACTTTACCCACCTAACAAGGTGCCAGCTCTTGAACATAATAATGAAGTCAAAGGAGAGAGTCTTGACTTAATTAAATATATTGACAGCAATTTTGAAGGACCTTCATTATTTCCTGATGATTCAGAAAAGAGAGAGATTGCTGAAGAGTTGATAAACTACGTGAATTCTTTCACTGGATCTGTGGTTTCTTCATTTAAAGGAGATGGAAATGAAGCTGATGTGGCGTTTGACCACATCGAATCCGCTCTTTCAAAATATGGAGATGGCCCATTTTTTCTAGGCCAGTTTAGTCTGGTGGATATAGCTTATGCTCCGTTTATTGAAAGGTTTCAGCCGTTCTTATTGGAAGTAAAAACATATGACATCACTGCTGGCAGGCCAAAACTAGCAGCTTGGATTGAGACCAGACTAAAAGAAGGCAAAGATCATGGCAATTGGACACTGGGGAGGGATGAGATGAACAAGATCGAGGGGTACCGACAAACAAGGGGCGATCCACAGGAACATGTTGATAGCTTTAAGAAGCGATTCTTGATGGATTATTGA

Coding sequence (CDS)

ATGTTCACTTCTTCCCTTCTTTCTTTGCTGTATGAGAAAATGGAGGGTATTTTCACCTTTTGTTTTGTGTGGGAAAGAGCTTTTCAGGAAGTGCTTCCTCCAGCCCTCACTTCATCTTCAGAACCACCTCCTATCTTTGATGGAACAACGAGGCTTTACATATCTTATACATGCCCTTATGCGCAGCGTGTGTGGATTACTCGGAACTGTAAGGGTTTGCAGAATAAGATACAATTGGTTCCAATAAATCTACAAGATAGGCCTTCTTGGTACAAGGAAAAACTTTACCCACCTAACAAGGTGCCAGCTCTTGAACATAATAATGAAGTCAAAGGAGAGAGTCTTGACTTAATTAAATATATTGACAGCAATTTTGAAGGACCTTCATTATTTCCTGATGATTCAGAAAAGAGAGAGATTGCTGAAGAGTTGATAAACTACGTGAATTCTTTCACTGGATCTGTGGTTTCTTCATTTAAAGGAGATGGAAATGAAGCTGATGTGGCGTTTGACCACATCGAATCCGCTCTTTCAAAATATGGAGATGGCCCATTTTTTCTAGGCCAGTTTAGTCTGGTGGATATAGCTTATGCTCCGTTTATTGAAAGGTTTCAGCCGTTCTTATTGGAAGTAAAAACATATGACATCACTGCTGGCAGGCCAAAACTAGCAGCTTGGATTGAGACCAGACTAAAAGAAGGCAAAGATCATGGCAATTGGACACTGGGGAGGGATGAGATGAACAAGATCGAGGGGTACCGACAAACAAGGGGCGATCCACAGGAACATGTTGATAGCTTTAAGAAGCGATTCTTGATGGATTATTGA

Protein sequence

MFTSSLLSLLYEKMEGIFTFCFVWERAFQEVLPPALTSSSEPPPIFDGTTRLYISYTCPYAQRVWITRNCKGLQNKIQLVPINLQDRPSWYKEKLYPPNKVPALEHNNEVKGESLDLIKYIDSNFEGPSLFPDDSEKREIAEELINYVNSFTGSVVSSFKGDGNEADVAFDHIESALSKYGDGPFFLGQFSLVDIAYAPFIERFQPFLLEVKTYDITAGRPKLAAWIETRLKEGKDHGNWTLGRDEMNKIEGYRQTRGDPQEHVDSFKKRFLMDY
Homology
BLAST of Lsi04G008290 vs. ExPASy Swiss-Prot
Match: Q9M2W2 (Glutathione S-transferase L2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=GSTL2 PE=2 SV=1)

HSP 1 Score: 306.2 bits (783), Expect = 3.7e-82
Identity = 152/238 (63.87%), Postives = 183/238 (76.89%), Query Frame = 0

Query: 34  PALTSSSEPPPIFDGTTRLYISYTCPYAQRVWITRNCKGLQNKIQLVPINLQDRPSWYKE 93
           P L SSSEP  +FDG+TRLYISYTCP+AQR WI RN KGLQNKI+LVPI+L++RP+WYKE
Sbjct: 65  PELDSSSEPVQVFDGSTRLYISYTCPFAQRAWIARNYKGLQNKIELVPIDLKNRPAWYKE 124

Query: 94  KLYPPNKVPALEHNNEVKGESLDLIKYIDSNFEGPSLFPDDSEKREIAEELINYVNSFTG 153
           K+Y  NKVPALEHNN V GESLDLIKYID+NFEGPSL PD  EK+ +A+EL++Y +SF+ 
Sbjct: 125 KVYSANKVPALEHNNRVLGESLDLIKYIDTNFEGPSLTPDGLEKQVVADELLSYTDSFSK 184

Query: 154 SVVSSFKG-DGNEADVAFDHIESALSKYGDGPFFLGQFSLVDIAYAPFIERFQPFLLEVK 213
           +V S+  G D N ADVAFD+IE ALSK+ +GPFFLGQFSLVD+AYAPFIERF+  L +V 
Sbjct: 185 AVRSTLNGTDTNAADVAFDYIEQALSKFNEGPFFLGQFSLVDVAYAPFIERFRLILSDVM 244

Query: 214 TYDITAGRPKLAAWIETRLKEGKDHGNWTLGRDEMNKIEGYRQTRGDPQEHVDSFKKR 271
             DIT+GRP LA WI+                 EMNKIE Y +TR DPQE V+ +K+R
Sbjct: 245 NVDITSGRPNLALWIQ-----------------EMNKIEAYTETRQDPQELVERYKRR 285

BLAST of Lsi04G008290 vs. ExPASy Swiss-Prot
Match: A1XBB7 (Protein IN2-1 homolog B OS=Oryza sativa subsp. indica OX=39946 GN=GSTZ5 PE=1 SV=1)

HSP 1 Score: 305.4 bits (781), Expect = 6.3e-82
Identity = 153/245 (62.45%), Postives = 185/245 (75.51%), Query Frame = 0

Query: 29  QEVLPPALTSSSEPPPIFDGTTRLYISYTCPYAQRVWITRNCKGLQNKIQLVPINLQDRP 88
           +EVLPP+LTSSSEPPP+FDGTTRLY++Y CPYAQR WI RN KGLQ+KI++V I+L DRP
Sbjct: 13  KEVLPPSLTSSSEPPPLFDGTTRLYVAYHCPYAQRAWIARNYKGLQDKIKIVAIDLADRP 72

Query: 89  SWYKEKLYPPNKVPALEHNNEVKGESLDLIKYIDSNFEGPSLFPDDSEKREIAEELINYV 148
           +WYKEK+YP NKVP+LEHNN+VKGESLDL+KYID+NFEGP+L PDDSEK++ AEEL+ Y 
Sbjct: 73  AWYKEKVYPENKVPSLEHNNQVKGESLDLVKYIDTNFEGPALLPDDSEKQQFAEELLAYT 132

Query: 149 NSFTGSVVSSF--KGD-GNEADVAFDHIESALSKYGDGPFFLGQFSLVDIAYAPFIERFQ 208
           ++F  +  SS   KGD  +EA  A D IE+ALSK+ DGPFFLGQFSLVDIAY PFIERFQ
Sbjct: 133 DAFNKASYSSIVAKGDVCDEAVAALDKIEAALSKFNDGPFFLGQFSLVDIAYVPFIERFQ 192

Query: 209 PFLLEVKTYDITAGRPKLAAWIETRLKEGKDHGNWTLGRDEMNKIEGYRQTRGDPQEHVD 268
            F   +K YDIT GRP L  +IE                 E+NKI  Y +T+ DPQ  ++
Sbjct: 193 IFFSGIKNYDITKGRPNLQKFIE-----------------EVNKIHAYTETKQDPQFLLE 240

Query: 269 SFKKR 271
             KKR
Sbjct: 253 HTKKR 240

BLAST of Lsi04G008290 vs. ExPASy Swiss-Prot
Match: Q8H8U5 (Protein IN2-1 homolog B OS=Oryza sativa subsp. japonica OX=39947 GN=GSTZ5 PE=2 SV=1)

HSP 1 Score: 305.4 bits (781), Expect = 6.3e-82
Identity = 153/245 (62.45%), Postives = 185/245 (75.51%), Query Frame = 0

Query: 29  QEVLPPALTSSSEPPPIFDGTTRLYISYTCPYAQRVWITRNCKGLQNKIQLVPINLQDRP 88
           +EVLPP+LTSSSEPPP+FDGTTRLY++Y CPYAQR WI RN KGLQ+KI++V I+L DRP
Sbjct: 13  KEVLPPSLTSSSEPPPLFDGTTRLYVAYHCPYAQRAWIARNYKGLQDKIKIVAIDLADRP 72

Query: 89  SWYKEKLYPPNKVPALEHNNEVKGESLDLIKYIDSNFEGPSLFPDDSEKREIAEELINYV 148
           +WYKEK+YP NKVP+LEHNN+VKGESLDL+KYID+NFEGP+L PDDSEK++ AEEL+ Y 
Sbjct: 73  AWYKEKVYPENKVPSLEHNNQVKGESLDLVKYIDTNFEGPALLPDDSEKQQFAEELLAYT 132

Query: 149 NSFTGSVVSSF--KGD-GNEADVAFDHIESALSKYGDGPFFLGQFSLVDIAYAPFIERFQ 208
           ++F  +  SS   KGD  +EA  A D IE+ALSK+ DGPFFLGQFSLVDIAY PFIERFQ
Sbjct: 133 DAFNKASYSSIVAKGDVCDEAVAALDKIEAALSKFNDGPFFLGQFSLVDIAYVPFIERFQ 192

Query: 209 PFLLEVKTYDITAGRPKLAAWIETRLKEGKDHGNWTLGRDEMNKIEGYRQTRGDPQEHVD 268
            F   +K YDIT GRP L  +IE                 E+NKI  Y +T+ DPQ  ++
Sbjct: 193 IFFSGIKNYDITKGRPNLQKFIE-----------------EVNKIHAYTETKQDPQFLLE 240

Query: 269 SFKKR 271
             KKR
Sbjct: 253 HTKKR 240

BLAST of Lsi04G008290 vs. ExPASy Swiss-Prot
Match: Q9LZ06 (Glutathione S-transferase L3 OS=Arabidopsis thaliana OX=3702 GN=GSTL3 PE=2 SV=1)

HSP 1 Score: 288.1 bits (736), Expect = 1.0e-76
Identity = 142/246 (57.72%), Postives = 178/246 (72.36%), Query Frame = 0

Query: 28  FQEVLPPALTSSSEPPPIFDGTTRLYISYTCPYAQRVWITRNCKGLQNKIQLVPINLQDR 87
           F E  P  L ++S+PP +FDGTTRLY SY CP+AQRVWITRN KGLQ KI+LVP++L +R
Sbjct: 7   FVEDRPAPLDATSDPPSLFDGTTRLYTSYVCPFAQRVWITRNFKGLQEKIKLVPLDLGNR 66

Query: 88  PSWYKEKLYPPNKVPALEHNNEVKGESLDLIKYIDSNFEGPSLFPDDSEKREIAEELINY 147
           P+WYKEK+YP NKVPALEHN ++ GESLDLIKY+D+ FEGPSL+P+D  KRE  +EL+ Y
Sbjct: 67  PAWYKEKVYPENKVPALEHNGKIIGESLDLIKYLDNTFEGPSLYPEDHAKREFGDELLKY 126

Query: 148 VNSFTGSVVSSFKGD-GNEADVAFDHIESALSKYGDGPFFLGQFSLVDIAYAPFIERFQP 207
            ++F  ++  S KGD   E     D++E+AL K+ DGPFFLGQ SLVDIAY PFIERFQ 
Sbjct: 127 TDTFVKTMYVSLKGDPSKETAPVLDYLENALYKFDDGPFFLGQLSLVDIAYIPFIERFQT 186

Query: 208 FLLEVKTYDITAGRPKLAAWIETRLKEGKDHGNWTLGRDEMNKIEGYRQTRGDPQEHVDS 267
            L E+   DITA RPKL+AWIE                 E+NK +GY QT+ DP+E V+ 
Sbjct: 187 VLNELFKCDITAERPKLSAWIE-----------------EINKSDGYAQTKMDPKEIVEV 235

Query: 268 FKKRFL 273
           FKK+F+
Sbjct: 247 FKKKFM 235

BLAST of Lsi04G008290 vs. ExPASy Swiss-Prot
Match: Q6NLB0 (Glutathione S-transferase L1 OS=Arabidopsis thaliana OX=3702 GN=GSTL1 PE=2 SV=1)

HSP 1 Score: 287.3 bits (734), Expect = 1.8e-76
Identity = 147/249 (59.04%), Postives = 182/249 (73.09%), Query Frame = 0

Query: 26  RAFQEVLPPALTSSSEPPPIFDGTTRLYISYTCPYAQRVWITRNCKGLQNKIQLVPINLQ 85
           + F E     L ++S+PP +FDGTTRLYISYTCP+AQRVWITRN KGLQ++I+LVPI+L 
Sbjct: 7   KIFVEDRQVPLDATSDPPALFDGTTRLYISYTCPFAQRVWITRNLKGLQDEIKLVPIDLP 66

Query: 86  DRPSWYKEKLYPPNKVPALEHNNEVKGESLDLIKYIDSNFEGPSLFPDDSEKREIAEELI 145
           +RP+W KEK+ P NKVPALEHN ++ GESLDLIKY+DSNF+GPSL+P+DS KRE  EEL+
Sbjct: 67  NRPAWLKEKVNPANKVPALEHNGKITGESLDLIKYVDSNFDGPSLYPEDSAKREFGEELL 126

Query: 146 NYVN-SFTGSVVSSFKGDG-NEADVAFDHIESALSKYGDGPFFLGQFSLVDIAYAPFIER 205
            YV+ +F  +V  SFKGD   E   AFDH+E+AL K+ DGPFFLG+ SLVDIAY PFIER
Sbjct: 127 KYVDETFVKTVFGSFKGDPVKETASAFDHVENALKKFDDGPFFLGELSLVDIAYIPFIER 186

Query: 206 FQPFLLEVKTYDITAGRPKLAAWIETRLKEGKDHGNWTLGRDEMNKIEGYRQTRGDPQEH 265
           FQ FL EV  Y+I  GRP LAAWIE                 +MNK+  Y QT+ D  E+
Sbjct: 187 FQVFLDEVFKYEIIIGRPNLAAWIE-----------------QMNKMVAYTQTKTD-SEY 237

Query: 266 VDSFKKRFL 273
           V ++ KRF+
Sbjct: 247 VVNYFKRFM 237

BLAST of Lsi04G008290 vs. ExPASy TrEMBL
Match: A0A1S3BDL2 (protein IN2-1 homolog B OS=Cucumis melo OX=3656 GN=LOC103488732 PE=4 SV=1)

HSP 1 Score: 442.2 bits (1136), Expect = 1.6e-120
Identity = 217/247 (87.85%), Postives = 225/247 (91.09%), Query Frame = 0

Query: 26  RAFQEVLPPALTSSSEPPPIFDGTTRLYISYTCPYAQRVWITRNCKGLQNKIQLVPINLQ 85
           +AFQEVLPPALTS SEPPPIFDGTTRLYISYTCPYAQRVWITRNCKGLQNKIQLVPINLQ
Sbjct: 65  QAFQEVLPPALTSVSEPPPIFDGTTRLYISYTCPYAQRVWITRNCKGLQNKIQLVPINLQ 124

Query: 86  DRPSWYKEKLYPPNKVPALEHNNEVKGESLDLIKYIDSNFEGPSLFPDDSEKREIAEELI 145
           DRPSWYKEK+YPPNKVPALEHNNEVKGESLDLIKYIDSNFEGPSLFPD+  KRE AEELI
Sbjct: 125 DRPSWYKEKVYPPNKVPALEHNNEVKGESLDLIKYIDSNFEGPSLFPDEPGKREFAEELI 184

Query: 146 NYVNSFTGSVVSSFKGDGNEADVAFDHIESALSKYGDGPFFLGQFSLVDIAYAPFIERFQ 205
           NYVNSFTGSVVSSFKGDGNEADVAFD+IESALSKYGDGPFFLGQFSLVDIAYAPFIERF+
Sbjct: 185 NYVNSFTGSVVSSFKGDGNEADVAFDYIESALSKYGDGPFFLGQFSLVDIAYAPFIERFR 244

Query: 206 PFLLEVKTYDITAGRPKLAAWIETRLKEGKDHGNWTLGRDEMNKIEGYRQTRGDPQEHVD 265
           PFLLEVKTYDITAGRPKLAAWIE                 EMNKI+GY+QTR DPQEHVD
Sbjct: 245 PFLLEVKTYDITAGRPKLAAWIE-----------------EMNKIKGYQQTRRDPQEHVD 294

Query: 266 SFKKRFL 273
           S+KKRFL
Sbjct: 305 SYKKRFL 294

BLAST of Lsi04G008290 vs. ExPASy TrEMBL
Match: A0A0A0KLG0 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G177050 PE=4 SV=1)

HSP 1 Score: 439.1 bits (1128), Expect = 1.3e-119
Identity = 215/247 (87.04%), Postives = 222/247 (89.88%), Query Frame = 0

Query: 26  RAFQEVLPPALTSSSEPPPIFDGTTRLYISYTCPYAQRVWITRNCKGLQNKIQLVPINLQ 85
           +AFQEVLPPALTS SEPPPIFDGTTRLYISYTCPYAQRVWITRNCKGLQN+IQLVPINLQ
Sbjct: 67  QAFQEVLPPALTSVSEPPPIFDGTTRLYISYTCPYAQRVWITRNCKGLQNRIQLVPINLQ 126

Query: 86  DRPSWYKEKLYPPNKVPALEHNNEVKGESLDLIKYIDSNFEGPSLFPDDSEKREIAEELI 145
           DRPSWYKEK+YPPNKVPALEHNNEVKGESLDLIKYIDSNFEGPSLFPD+ EKRE AEELI
Sbjct: 127 DRPSWYKEKVYPPNKVPALEHNNEVKGESLDLIKYIDSNFEGPSLFPDEPEKREFAEELI 186

Query: 146 NYVNSFTGSVVSSFKGDGNEADVAFDHIESALSKYGDGPFFLGQFSLVDIAYAPFIERFQ 205
           NYVNSFTGSVVSSFKGDGNEAD  FD+IESALSKYGDGPFFLGQFSLVDIAYAPFIERF+
Sbjct: 187 NYVNSFTGSVVSSFKGDGNEADATFDYIESALSKYGDGPFFLGQFSLVDIAYAPFIERFR 246

Query: 206 PFLLEVKTYDITAGRPKLAAWIETRLKEGKDHGNWTLGRDEMNKIEGYRQTRGDPQEHVD 265
           PFLLEVKTYDITAGRPKLAAWIE                 E N IEGYRQTR DPQEHVD
Sbjct: 247 PFLLEVKTYDITAGRPKLAAWIE-----------------ETNMIEGYRQTRRDPQEHVD 296

Query: 266 SFKKRFL 273
           S+KKRFL
Sbjct: 307 SYKKRFL 296

BLAST of Lsi04G008290 vs. ExPASy TrEMBL
Match: A0A6J1JYS4 (protein IN2-1 homolog B OS=Cucurbita maxima OX=3661 GN=LOC111489100 PE=4 SV=1)

HSP 1 Score: 433.7 bits (1114), Expect = 5.7e-118
Identity = 210/246 (85.37%), Postives = 224/246 (91.06%), Query Frame = 0

Query: 27  AFQEVLPPALTSSSEPPPIFDGTTRLYISYTCPYAQRVWITRNCKGLQNKIQLVPINLQD 86
           AFQEVLPPALTS+SEPPPIFDGTTRLYISYTCPYAQRVWITRNCKGLQNKIQLVPINL+D
Sbjct: 67  AFQEVLPPALTSASEPPPIFDGTTRLYISYTCPYAQRVWITRNCKGLQNKIQLVPINLRD 126

Query: 87  RPSWYKEKLYPPNKVPALEHNNEVKGESLDLIKYIDSNFEGPSLFPDDSEKREIAEELIN 146
           RPSWYKEK+YPPNKVPALEHNNEVKGESLDLI+YIDSNFEGPSLFP+D  +RE AEEL+N
Sbjct: 127 RPSWYKEKVYPPNKVPALEHNNEVKGESLDLIRYIDSNFEGPSLFPEDQGRREFAEELMN 186

Query: 147 YVNSFTGSVVSSFKGDGNEADVAFDHIESALSKYGDGPFFLGQFSLVDIAYAPFIERFQP 206
           YVNSF GSVVSSFKGD NEADVAFD+IESALSKYGDGPFFLGQFSLVDIAYAPFIER+QP
Sbjct: 187 YVNSFRGSVVSSFKGDRNEADVAFDYIESALSKYGDGPFFLGQFSLVDIAYAPFIERYQP 246

Query: 207 FLLEVKTYDITAGRPKLAAWIETRLKEGKDHGNWTLGRDEMNKIEGYRQTRGDPQEHVDS 266
           FLLEVKTYDITAGRPKLAAWIE                 EMNKIEGY+QT+GDP+EHVD+
Sbjct: 247 FLLEVKTYDITAGRPKLAAWIE-----------------EMNKIEGYQQTKGDPREHVDN 295

Query: 267 FKKRFL 273
           F+KRFL
Sbjct: 307 FRKRFL 295

BLAST of Lsi04G008290 vs. ExPASy TrEMBL
Match: A0A6J1GZ79 (protein IN2-1 homolog B isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111458219 PE=4 SV=1)

HSP 1 Score: 428.3 bits (1100), Expect = 2.4e-116
Identity = 206/245 (84.08%), Postives = 222/245 (90.61%), Query Frame = 0

Query: 28  FQEVLPPALTSSSEPPPIFDGTTRLYISYTCPYAQRVWITRNCKGLQNKIQLVPINLQDR 87
           FQEVLPPALTS+SEPPPIFDGTTRLYISY+CPYAQRVWITRNCKGLQ KI+LVPINLQDR
Sbjct: 68  FQEVLPPALTSASEPPPIFDGTTRLYISYSCPYAQRVWITRNCKGLQTKIELVPINLQDR 127

Query: 88  PSWYKEKLYPPNKVPALEHNNEVKGESLDLIKYIDSNFEGPSLFPDDSEKREIAEELINY 147
           PSWYKEK+YPPNKVPALEHNNEVKGESLDLI+YIDSNFEGPSLFP+D  +RE AEEL+NY
Sbjct: 128 PSWYKEKVYPPNKVPALEHNNEVKGESLDLIRYIDSNFEGPSLFPEDQGRREFAEELMNY 187

Query: 148 VNSFTGSVVSSFKGDGNEADVAFDHIESALSKYGDGPFFLGQFSLVDIAYAPFIERFQPF 207
           +NSF GSVVSSFKGD NEADVAFD+IESALSKYGDGPFFLGQFSLVDIAYAPFIER+QPF
Sbjct: 188 LNSFRGSVVSSFKGDRNEADVAFDYIESALSKYGDGPFFLGQFSLVDIAYAPFIERYQPF 247

Query: 208 LLEVKTYDITAGRPKLAAWIETRLKEGKDHGNWTLGRDEMNKIEGYRQTRGDPQEHVDSF 267
           LLEVKTYDITAGRPKLAAWIE                 EMNK+EGY+QT+GDP+EHVD+F
Sbjct: 248 LLEVKTYDITAGRPKLAAWIE-----------------EMNKVEGYQQTKGDPREHVDNF 295

Query: 268 KKRFL 273
           KKRFL
Sbjct: 308 KKRFL 295

BLAST of Lsi04G008290 vs. ExPASy TrEMBL
Match: A0A6J1GXY4 (protein IN2-1 homolog B isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111458219 PE=4 SV=1)

HSP 1 Score: 421.4 bits (1082), Expect = 2.9e-114
Identity = 205/245 (83.67%), Postives = 221/245 (90.20%), Query Frame = 0

Query: 28  FQEVLPPALTSSSEPPPIFDGTTRLYISYTCPYAQRVWITRNCKGLQNKIQLVPINLQDR 87
           FQEVLPPALTS+SEPPPIFDGTTRLYISY+CPYAQRVWITRNCKGLQ KI+LVPINLQDR
Sbjct: 68  FQEVLPPALTSASEPPPIFDGTTRLYISYSCPYAQRVWITRNCKGLQTKIELVPINLQDR 127

Query: 88  PSWYKEKLYPPNKVPALEHNNEVKGESLDLIKYIDSNFEGPSLFPDDSEKREIAEELINY 147
           PSWYKEK+YPPNKVPALEHNNEVKGESLDLI+YIDSNFEGPSLFP+   +RE AEEL+NY
Sbjct: 128 PSWYKEKVYPPNKVPALEHNNEVKGESLDLIRYIDSNFEGPSLFPEG--RREFAEELMNY 187

Query: 148 VNSFTGSVVSSFKGDGNEADVAFDHIESALSKYGDGPFFLGQFSLVDIAYAPFIERFQPF 207
           +NSF GSVVSSFKGD NEADVAFD+IESALSKYGDGPFFLGQFSLVDIAYAPFIER+QPF
Sbjct: 188 LNSFRGSVVSSFKGDRNEADVAFDYIESALSKYGDGPFFLGQFSLVDIAYAPFIERYQPF 247

Query: 208 LLEVKTYDITAGRPKLAAWIETRLKEGKDHGNWTLGRDEMNKIEGYRQTRGDPQEHVDSF 267
           LLEVKTYDITAGRPKLAAWIE                 EMNK+EGY+QT+GDP+EHVD+F
Sbjct: 248 LLEVKTYDITAGRPKLAAWIE-----------------EMNKVEGYQQTKGDPREHVDNF 293

Query: 268 KKRFL 273
           KKRFL
Sbjct: 308 KKRFL 293

BLAST of Lsi04G008290 vs. NCBI nr
Match: XP_008445827.1 (PREDICTED: protein IN2-1 homolog B [Cucumis melo])

HSP 1 Score: 442.2 bits (1136), Expect = 3.3e-120
Identity = 217/247 (87.85%), Postives = 225/247 (91.09%), Query Frame = 0

Query: 26  RAFQEVLPPALTSSSEPPPIFDGTTRLYISYTCPYAQRVWITRNCKGLQNKIQLVPINLQ 85
           +AFQEVLPPALTS SEPPPIFDGTTRLYISYTCPYAQRVWITRNCKGLQNKIQLVPINLQ
Sbjct: 65  QAFQEVLPPALTSVSEPPPIFDGTTRLYISYTCPYAQRVWITRNCKGLQNKIQLVPINLQ 124

Query: 86  DRPSWYKEKLYPPNKVPALEHNNEVKGESLDLIKYIDSNFEGPSLFPDDSEKREIAEELI 145
           DRPSWYKEK+YPPNKVPALEHNNEVKGESLDLIKYIDSNFEGPSLFPD+  KRE AEELI
Sbjct: 125 DRPSWYKEKVYPPNKVPALEHNNEVKGESLDLIKYIDSNFEGPSLFPDEPGKREFAEELI 184

Query: 146 NYVNSFTGSVVSSFKGDGNEADVAFDHIESALSKYGDGPFFLGQFSLVDIAYAPFIERFQ 205
           NYVNSFTGSVVSSFKGDGNEADVAFD+IESALSKYGDGPFFLGQFSLVDIAYAPFIERF+
Sbjct: 185 NYVNSFTGSVVSSFKGDGNEADVAFDYIESALSKYGDGPFFLGQFSLVDIAYAPFIERFR 244

Query: 206 PFLLEVKTYDITAGRPKLAAWIETRLKEGKDHGNWTLGRDEMNKIEGYRQTRGDPQEHVD 265
           PFLLEVKTYDITAGRPKLAAWIE                 EMNKI+GY+QTR DPQEHVD
Sbjct: 245 PFLLEVKTYDITAGRPKLAAWIE-----------------EMNKIKGYQQTRRDPQEHVD 294

Query: 266 SFKKRFL 273
           S+KKRFL
Sbjct: 305 SYKKRFL 294

BLAST of Lsi04G008290 vs. NCBI nr
Match: XP_004143598.1 (protein IN2-1 homolog B isoform X1 [Cucumis sativus] >KGN50485.1 hypothetical protein Csa_000618 [Cucumis sativus])

HSP 1 Score: 439.1 bits (1128), Expect = 2.8e-119
Identity = 215/247 (87.04%), Postives = 222/247 (89.88%), Query Frame = 0

Query: 26  RAFQEVLPPALTSSSEPPPIFDGTTRLYISYTCPYAQRVWITRNCKGLQNKIQLVPINLQ 85
           +AFQEVLPPALTS SEPPPIFDGTTRLYISYTCPYAQRVWITRNCKGLQN+IQLVPINLQ
Sbjct: 67  QAFQEVLPPALTSVSEPPPIFDGTTRLYISYTCPYAQRVWITRNCKGLQNRIQLVPINLQ 126

Query: 86  DRPSWYKEKLYPPNKVPALEHNNEVKGESLDLIKYIDSNFEGPSLFPDDSEKREIAEELI 145
           DRPSWYKEK+YPPNKVPALEHNNEVKGESLDLIKYIDSNFEGPSLFPD+ EKRE AEELI
Sbjct: 127 DRPSWYKEKVYPPNKVPALEHNNEVKGESLDLIKYIDSNFEGPSLFPDEPEKREFAEELI 186

Query: 146 NYVNSFTGSVVSSFKGDGNEADVAFDHIESALSKYGDGPFFLGQFSLVDIAYAPFIERFQ 205
           NYVNSFTGSVVSSFKGDGNEAD  FD+IESALSKYGDGPFFLGQFSLVDIAYAPFIERF+
Sbjct: 187 NYVNSFTGSVVSSFKGDGNEADATFDYIESALSKYGDGPFFLGQFSLVDIAYAPFIERFR 246

Query: 206 PFLLEVKTYDITAGRPKLAAWIETRLKEGKDHGNWTLGRDEMNKIEGYRQTRGDPQEHVD 265
           PFLLEVKTYDITAGRPKLAAWIE                 E N IEGYRQTR DPQEHVD
Sbjct: 247 PFLLEVKTYDITAGRPKLAAWIE-----------------ETNMIEGYRQTRRDPQEHVD 296

Query: 266 SFKKRFL 273
           S+KKRFL
Sbjct: 307 SYKKRFL 296

BLAST of Lsi04G008290 vs. NCBI nr
Match: XP_023526395.1 (protein IN2-1 homolog B isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 434.1 bits (1115), Expect = 9.0e-118
Identity = 210/246 (85.37%), Postives = 224/246 (91.06%), Query Frame = 0

Query: 27  AFQEVLPPALTSSSEPPPIFDGTTRLYISYTCPYAQRVWITRNCKGLQNKIQLVPINLQD 86
           AFQEVLPPALTS+SEPPPIFDGTTRLYISYTCPYAQRVWITRNCKGLQNKIQLVPINL+D
Sbjct: 67  AFQEVLPPALTSASEPPPIFDGTTRLYISYTCPYAQRVWITRNCKGLQNKIQLVPINLRD 126

Query: 87  RPSWYKEKLYPPNKVPALEHNNEVKGESLDLIKYIDSNFEGPSLFPDDSEKREIAEELIN 146
           RPSWYKEK+YPPNKVPALEHNNEVKGESLDLI+YIDSNFEGPSLFP+D  +RE AEEL+N
Sbjct: 127 RPSWYKEKVYPPNKVPALEHNNEVKGESLDLIRYIDSNFEGPSLFPEDQGRREFAEELMN 186

Query: 147 YVNSFTGSVVSSFKGDGNEADVAFDHIESALSKYGDGPFFLGQFSLVDIAYAPFIERFQP 206
           YVNSF GSVVSSFKGD NEADVAFD+IESALSKYGDGPFF+GQFSLVDIAYAPFIER+QP
Sbjct: 187 YVNSFRGSVVSSFKGDRNEADVAFDYIESALSKYGDGPFFIGQFSLVDIAYAPFIERYQP 246

Query: 207 FLLEVKTYDITAGRPKLAAWIETRLKEGKDHGNWTLGRDEMNKIEGYRQTRGDPQEHVDS 266
           FLLEVKTYDITAGRPKLAAWIE                 EMNKIEGY+QT+GDP+EHVD+
Sbjct: 247 FLLEVKTYDITAGRPKLAAWIE-----------------EMNKIEGYQQTKGDPREHVDN 295

Query: 267 FKKRFL 273
           FKKRFL
Sbjct: 307 FKKRFL 295

BLAST of Lsi04G008290 vs. NCBI nr
Match: XP_022992909.1 (protein IN2-1 homolog B [Cucurbita maxima])

HSP 1 Score: 433.7 bits (1114), Expect = 1.2e-117
Identity = 210/246 (85.37%), Postives = 224/246 (91.06%), Query Frame = 0

Query: 27  AFQEVLPPALTSSSEPPPIFDGTTRLYISYTCPYAQRVWITRNCKGLQNKIQLVPINLQD 86
           AFQEVLPPALTS+SEPPPIFDGTTRLYISYTCPYAQRVWITRNCKGLQNKIQLVPINL+D
Sbjct: 67  AFQEVLPPALTSASEPPPIFDGTTRLYISYTCPYAQRVWITRNCKGLQNKIQLVPINLRD 126

Query: 87  RPSWYKEKLYPPNKVPALEHNNEVKGESLDLIKYIDSNFEGPSLFPDDSEKREIAEELIN 146
           RPSWYKEK+YPPNKVPALEHNNEVKGESLDLI+YIDSNFEGPSLFP+D  +RE AEEL+N
Sbjct: 127 RPSWYKEKVYPPNKVPALEHNNEVKGESLDLIRYIDSNFEGPSLFPEDQGRREFAEELMN 186

Query: 147 YVNSFTGSVVSSFKGDGNEADVAFDHIESALSKYGDGPFFLGQFSLVDIAYAPFIERFQP 206
           YVNSF GSVVSSFKGD NEADVAFD+IESALSKYGDGPFFLGQFSLVDIAYAPFIER+QP
Sbjct: 187 YVNSFRGSVVSSFKGDRNEADVAFDYIESALSKYGDGPFFLGQFSLVDIAYAPFIERYQP 246

Query: 207 FLLEVKTYDITAGRPKLAAWIETRLKEGKDHGNWTLGRDEMNKIEGYRQTRGDPQEHVDS 266
           FLLEVKTYDITAGRPKLAAWIE                 EMNKIEGY+QT+GDP+EHVD+
Sbjct: 247 FLLEVKTYDITAGRPKLAAWIE-----------------EMNKIEGYQQTKGDPREHVDN 295

Query: 267 FKKRFL 273
           F+KRFL
Sbjct: 307 FRKRFL 295

BLAST of Lsi04G008290 vs. NCBI nr
Match: KAG6601035.1 (putative protein phosphatase 2C 38, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 428.3 bits (1100), Expect = 4.9e-116
Identity = 206/246 (83.74%), Postives = 223/246 (90.65%), Query Frame = 0

Query: 27  AFQEVLPPALTSSSEPPPIFDGTTRLYISYTCPYAQRVWITRNCKGLQNKIQLVPINLQD 86
           AF+EVLPPALTS+SEPPPIFDGTTRLYISY+CPYAQRVWITRNCKGLQ KI+LVPINLQD
Sbjct: 383 AFREVLPPALTSASEPPPIFDGTTRLYISYSCPYAQRVWITRNCKGLQTKIELVPINLQD 442

Query: 87  RPSWYKEKLYPPNKVPALEHNNEVKGESLDLIKYIDSNFEGPSLFPDDSEKREIAEELIN 146
           RPSWYKEK+YPPNKVPALEHNNEVKGESLDLI+YIDSNFEGPSLFP+D  +RE AEEL+N
Sbjct: 443 RPSWYKEKVYPPNKVPALEHNNEVKGESLDLIRYIDSNFEGPSLFPEDQGRREFAEELMN 502

Query: 147 YVNSFTGSVVSSFKGDGNEADVAFDHIESALSKYGDGPFFLGQFSLVDIAYAPFIERFQP 206
           Y+NSF GSVVSSFKGD NEADVAFD+IESALSKYGDGPFFLGQFSLVDIAYAPFIER+QP
Sbjct: 503 YLNSFRGSVVSSFKGDRNEADVAFDYIESALSKYGDGPFFLGQFSLVDIAYAPFIERYQP 562

Query: 207 FLLEVKTYDITAGRPKLAAWIETRLKEGKDHGNWTLGRDEMNKIEGYRQTRGDPQEHVDS 266
           FLLEVKTYDITAGRPKLAAWIE                 EMNK+EGY+QT+GDP+EHVD+
Sbjct: 563 FLLEVKTYDITAGRPKLAAWIE-----------------EMNKVEGYQQTKGDPREHVDN 611

Query: 267 FKKRFL 273
           FKKRFL
Sbjct: 623 FKKRFL 611

BLAST of Lsi04G008290 vs. TAIR 10
Match: AT3G55040.1 (glutathione transferase lambda 2 )

HSP 1 Score: 306.2 bits (783), Expect = 2.6e-83
Identity = 152/238 (63.87%), Postives = 183/238 (76.89%), Query Frame = 0

Query: 34  PALTSSSEPPPIFDGTTRLYISYTCPYAQRVWITRNCKGLQNKIQLVPINLQDRPSWYKE 93
           P L SSSEP  +FDG+TRLYISYTCP+AQR WI RN KGLQNKI+LVPI+L++RP+WYKE
Sbjct: 65  PELDSSSEPVQVFDGSTRLYISYTCPFAQRAWIARNYKGLQNKIELVPIDLKNRPAWYKE 124

Query: 94  KLYPPNKVPALEHNNEVKGESLDLIKYIDSNFEGPSLFPDDSEKREIAEELINYVNSFTG 153
           K+Y  NKVPALEHNN V GESLDLIKYID+NFEGPSL PD  EK+ +A+EL++Y +SF+ 
Sbjct: 125 KVYSANKVPALEHNNRVLGESLDLIKYIDTNFEGPSLTPDGLEKQVVADELLSYTDSFSK 184

Query: 154 SVVSSFKG-DGNEADVAFDHIESALSKYGDGPFFLGQFSLVDIAYAPFIERFQPFLLEVK 213
           +V S+  G D N ADVAFD+IE ALSK+ +GPFFLGQFSLVD+AYAPFIERF+  L +V 
Sbjct: 185 AVRSTLNGTDTNAADVAFDYIEQALSKFNEGPFFLGQFSLVDVAYAPFIERFRLILSDVM 244

Query: 214 TYDITAGRPKLAAWIETRLKEGKDHGNWTLGRDEMNKIEGYRQTRGDPQEHVDSFKKR 271
             DIT+GRP LA WI+                 EMNKIE Y +TR DPQE V+ +K+R
Sbjct: 245 NVDITSGRPNLALWIQ-----------------EMNKIEAYTETRQDPQELVERYKRR 285

BLAST of Lsi04G008290 vs. TAIR 10
Match: AT5G02790.1 (Glutathione S-transferase family protein )

HSP 1 Score: 288.1 bits (736), Expect = 7.4e-78
Identity = 142/246 (57.72%), Postives = 178/246 (72.36%), Query Frame = 0

Query: 28  FQEVLPPALTSSSEPPPIFDGTTRLYISYTCPYAQRVWITRNCKGLQNKIQLVPINLQDR 87
           F E  P  L ++S+PP +FDGTTRLY SY CP+AQRVWITRN KGLQ KI+LVP++L +R
Sbjct: 7   FVEDRPAPLDATSDPPSLFDGTTRLYTSYVCPFAQRVWITRNFKGLQEKIKLVPLDLGNR 66

Query: 88  PSWYKEKLYPPNKVPALEHNNEVKGESLDLIKYIDSNFEGPSLFPDDSEKREIAEELINY 147
           P+WYKEK+YP NKVPALEHN ++ GESLDLIKY+D+ FEGPSL+P+D  KRE  +EL+ Y
Sbjct: 67  PAWYKEKVYPENKVPALEHNGKIIGESLDLIKYLDNTFEGPSLYPEDHAKREFGDELLKY 126

Query: 148 VNSFTGSVVSSFKGD-GNEADVAFDHIESALSKYGDGPFFLGQFSLVDIAYAPFIERFQP 207
            ++F  ++  S KGD   E     D++E+AL K+ DGPFFLGQ SLVDIAY PFIERFQ 
Sbjct: 127 TDTFVKTMYVSLKGDPSKETAPVLDYLENALYKFDDGPFFLGQLSLVDIAYIPFIERFQT 186

Query: 208 FLLEVKTYDITAGRPKLAAWIETRLKEGKDHGNWTLGRDEMNKIEGYRQTRGDPQEHVDS 267
            L E+   DITA RPKL+AWIE                 E+NK +GY QT+ DP+E V+ 
Sbjct: 187 VLNELFKCDITAERPKLSAWIE-----------------EINKSDGYAQTKMDPKEIVEV 235

Query: 268 FKKRFL 273
           FKK+F+
Sbjct: 247 FKKKFM 235

BLAST of Lsi04G008290 vs. TAIR 10
Match: AT5G02780.1 (glutathione transferase lambda 1 )

HSP 1 Score: 287.3 bits (734), Expect = 1.3e-77
Identity = 147/249 (59.04%), Postives = 182/249 (73.09%), Query Frame = 0

Query: 26  RAFQEVLPPALTSSSEPPPIFDGTTRLYISYTCPYAQRVWITRNCKGLQNKIQLVPINLQ 85
           + F E     L ++S+PP +FDGTTRLYISYTCP+AQRVWITRN KGLQ++I+LVPI+L 
Sbjct: 7   KIFVEDRQVPLDATSDPPALFDGTTRLYISYTCPFAQRVWITRNLKGLQDEIKLVPIDLP 66

Query: 86  DRPSWYKEKLYPPNKVPALEHNNEVKGESLDLIKYIDSNFEGPSLFPDDSEKREIAEELI 145
           +RP+W KEK+ P NKVPALEHN ++ GESLDLIKY+DSNF+GPSL+P+DS KRE  EEL+
Sbjct: 67  NRPAWLKEKVNPANKVPALEHNGKITGESLDLIKYVDSNFDGPSLYPEDSAKREFGEELL 126

Query: 146 NYVN-SFTGSVVSSFKGDG-NEADVAFDHIESALSKYGDGPFFLGQFSLVDIAYAPFIER 205
            YV+ +F  +V  SFKGD   E   AFDH+E+AL K+ DGPFFLG+ SLVDIAY PFIER
Sbjct: 127 KYVDETFVKTVFGSFKGDPVKETASAFDHVENALKKFDDGPFFLGELSLVDIAYIPFIER 186

Query: 206 FQPFLLEVKTYDITAGRPKLAAWIETRLKEGKDHGNWTLGRDEMNKIEGYRQTRGDPQEH 265
           FQ FL EV  Y+I  GRP LAAWIE                 +MNK+  Y QT+ D  E+
Sbjct: 187 FQVFLDEVFKYEIIIGRPNLAAWIE-----------------QMNKMVAYTQTKTD-SEY 237

Query: 266 VDSFKKRFL 273
           V ++ KRF+
Sbjct: 247 VVNYFKRFM 237

BLAST of Lsi04G008290 vs. TAIR 10
Match: AT5G02780.2 (glutathione transferase lambda 1 )

HSP 1 Score: 287.0 bits (733), Expect = 1.6e-77
Identity = 146/248 (58.87%), Postives = 183/248 (73.79%), Query Frame = 0

Query: 26  RAFQEVLPPALTSSSEPPPIFDGTTRLYISYTCPYAQRVWITRNCKGLQNKIQLVPINLQ 85
           + F E     L ++S+PP +FDGTTRLYISYTCP+AQRVWITRN KGLQ++I+LVPI+L 
Sbjct: 7   KIFVEDRQVPLDATSDPPALFDGTTRLYISYTCPFAQRVWITRNLKGLQDEIKLVPIDLP 66

Query: 86  DRPSWYKEKLYPPNKVPALEHNNEVKGESLDLIKYIDSNFEGPSLFPDDSEKREIAEELI 145
           +RP+W KEK+ P NKVPALEHN ++ GESLDLIKY+DSNF+GPSL+P+DS KRE  EEL+
Sbjct: 67  NRPAWLKEKVNPANKVPALEHNGKITGESLDLIKYVDSNFDGPSLYPEDSAKREFGEELL 126

Query: 146 NYVN-SFTGSVVSSFKGDGNEADVAFDHIESALSKYGDGPFFLGQFSLVDIAYAPFIERF 205
            YV+ +F  +V  SFKGD  + + AFDH+E+AL K+ DGPFFLG+ SLVDIAY PFIERF
Sbjct: 127 KYVDETFVKTVFGSFKGDPVK-ETAFDHVENALKKFDDGPFFLGELSLVDIAYIPFIERF 186

Query: 206 QPFLLEVKTYDITAGRPKLAAWIETRLKEGKDHGNWTLGRDEMNKIEGYRQTRGDPQEHV 265
           Q FL EV  Y+I  GRP LAAWIE                 +MNK+  Y QT+ D  E+V
Sbjct: 187 QVFLDEVFKYEIIIGRPNLAAWIE-----------------QMNKMVAYTQTKTD-SEYV 235

Query: 266 DSFKKRFL 273
            ++ KRF+
Sbjct: 247 VNYFKRFM 235

BLAST of Lsi04G008290 vs. TAIR 10
Match: AT1G78370.1 (glutathione S-transferase TAU 20 )

HSP 1 Score: 52.8 bits (125), Expect = 5.2e-07
Identity = 40/134 (29.85%), Postives = 64/134 (47.76%), Query Frame = 0

Query: 100 KVPALEHNNEVKGESLDLIKYIDSNF-EGPSLFPDDSEKREIAEELINYVN-SFTGSVVS 159
           K+P L HN +   ESL++++Y+D  + E    FP D   R  A    ++V+  FT +   
Sbjct: 53  KIPVLVHNGKPVCESLNVVQYVDEAWPEKNPFFPSDPYGRAQARFWADFVDKKFTDAQFK 112

Query: 160 SFKGDGNEADVA----FDHIESALSKYGDGPFFLG-QFSLVDIAYAPFIERFQPFLLEVK 219
            +   G E +       + ++   S+ GD P+F G  F  VDI+   F   FQ +  +  
Sbjct: 113 VWGKKGEEQEAGKKEFIEAVKILESELGDKPYFGGDSFGYVDISLITFSSWFQAY-EKFG 172

Query: 220 TYDITAGRPKLAAW 227
            + I +  PKL AW
Sbjct: 173 NFSIESESPKLIAW 185

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9M2W23.7e-8263.87Glutathione S-transferase L2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=G... [more]
A1XBB76.3e-8262.45Protein IN2-1 homolog B OS=Oryza sativa subsp. indica OX=39946 GN=GSTZ5 PE=1 SV=... [more]
Q8H8U56.3e-8262.45Protein IN2-1 homolog B OS=Oryza sativa subsp. japonica OX=39947 GN=GSTZ5 PE=2 S... [more]
Q9LZ061.0e-7657.72Glutathione S-transferase L3 OS=Arabidopsis thaliana OX=3702 GN=GSTL3 PE=2 SV=1[more]
Q6NLB01.8e-7659.04Glutathione S-transferase L1 OS=Arabidopsis thaliana OX=3702 GN=GSTL1 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A1S3BDL21.6e-12087.85protein IN2-1 homolog B OS=Cucumis melo OX=3656 GN=LOC103488732 PE=4 SV=1[more]
A0A0A0KLG01.3e-11987.04Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G177050 PE=4 SV=1[more]
A0A6J1JYS45.7e-11885.37protein IN2-1 homolog B OS=Cucurbita maxima OX=3661 GN=LOC111489100 PE=4 SV=1[more]
A0A6J1GZ792.4e-11684.08protein IN2-1 homolog B isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111458219... [more]
A0A6J1GXY42.9e-11483.67protein IN2-1 homolog B isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111458219... [more]
Match NameE-valueIdentityDescription
XP_008445827.13.3e-12087.85PREDICTED: protein IN2-1 homolog B [Cucumis melo][more]
XP_004143598.12.8e-11987.04protein IN2-1 homolog B isoform X1 [Cucumis sativus] >KGN50485.1 hypothetical pr... [more]
XP_023526395.19.0e-11885.37protein IN2-1 homolog B isoform X1 [Cucurbita pepo subsp. pepo][more]
XP_022992909.11.2e-11785.37protein IN2-1 homolog B [Cucurbita maxima][more]
KAG6601035.14.9e-11683.74putative protein phosphatase 2C 38, partial [Cucurbita argyrosperma subsp. soror... [more]
Match NameE-valueIdentityDescription
AT3G55040.12.6e-8363.87glutathione transferase lambda 2 [more]
AT5G02790.17.4e-7857.72Glutathione S-transferase family protein [more]
AT5G02780.11.3e-7759.04glutathione transferase lambda 1 [more]
AT5G02780.21.6e-7758.87glutathione transferase lambda 1 [more]
AT1G78370.15.2e-0729.85glutathione S-transferase TAU 20 [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR004045Glutathione S-transferase, N-terminalPFAMPF13417GST_N_3coord: 52..128
e-value: 1.1E-19
score: 70.6
IPR004045Glutathione S-transferase, N-terminalPROSITEPS50404GST_NTERcoord: 48..129
score: 12.204751
NoneNo IPR availableGENE3D3.40.30.10Glutaredoxincoord: 25..126
e-value: 3.0E-46
score: 157.5
NoneNo IPR availablePFAMPF13410GST_C_2coord: 169..228
e-value: 6.0E-9
score: 35.8
NoneNo IPR availableGENE3D1.20.1050.10coord: 127..275
e-value: 3.4E-52
score: 178.0
NoneNo IPR availablePANTHERPTHR44328:SF11GLUTATHIONE S-TRANSFERASE L2, CHLOROPLASTICcoord: 26..272
IPR040079Glutathione Transferase familySFLDSFLDS00019Glutathione_Transferase_(cytocoord: 52..229
e-value: 1.6E-20
score: 68.3
IPR044629Glutathione S-transferase L1/2/3PANTHERPTHR44328GLUTATHIONE S-TRANSFERASE L1coord: 26..272
IPR036282Glutathione S-transferase, C-terminal domain superfamilySUPERFAMILY47616GST C-terminal domain-likecoord: 125..230
IPR036249Thioredoxin-like superfamilySUPERFAMILY52833Thioredoxin-likecoord: 38..130

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi04G008290.1Lsi04G008290.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006749 glutathione metabolic process
biological_process GO:0010731 protein glutathionylation
molecular_function GO:0004364 glutathione transferase activity
molecular_function GO:0005515 protein binding