Lsi04G006260 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi04G006260
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	Pectin lyase-like superfamily protein, putative
Location	chr04: 5730924 .. 5731832 (-)
RNA-Seq Expression	Lsi04G006260
Synteny	Lsi04G006260

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCATGGGAAAAAGGTTGTGGATATGATGGTTCTGCAGTGGTTTTAATACCACAAGGCACCTTCTTGGTTTTCCCAATGAAACTTGAAGGTCCTTGCCATGGCTCAATTAAACTACAGATCGACGGCAATGTCTTGGCTCCTCACGATAAACTTTTCATCAAGGGTGAATACTGGCTCGCCCTCCATCAGGTCAACAATTTGATCGTCGACGGTCAAGGGGGGTTCGACGGCCAAGGCTCTTCCGCTTGGGGTTATAGAGGTCACGACCGTCCAATCGTAAGTTTATTTTCATTTCTTTTAGTTCACAAATTGAGGATAACTCAACTAGTTTAGATTAGAGATTTAAATTCGATTACGTTTTTTGATTTCATGCAGACATTAAAATTTACTGCAATCAACAATGCGACGATAAGGAACATCAAGTCTGTGGACAGTAAATTCTTCCATTTTGCAGTGCACGAAAGCGAAAACATGACGTTTGATAATGTCACACTCATAGCTCCAAAAAACAGTTCCAACACGGATGGAATCCACATCTCCAAATCAACTAACATCAACATCTTCAACTCAGTGATGAGCACAGGAGACGATTGCATCTCCATGGGTCATGGAAGCCAAAACATCAATATAACCAATGTTCAATGTGGTCCAGGCCACGGAATCAGCATCGGAAGCCTTGGCCAAGATCCGAAAGAATTAAATGTGACCAAAATCACCGTAAGACACTCCATATTCAAAAATACCAAAAATGGTGTGAGGATCAAATCATGGTCCACCCCAAATCCAAGTATAGTTACCAAAGTTTCATTCATCCATCTTCAAATGGAGAATGTCGAAAATCCTATCATCATCGATCAAAACTATTGCCCTCATTGCTCTAGCAATCATCAACATCAACTGGTATAA

mRNA sequence

ATGGCATGGGAAAAAGGTTGTGGATATGATGGTTCTGCAGTGGTTTTAATACCACAAGGCACCTTCTTGGTTTTCCCAATGAAACTTGAAGGTCCTTGCCATGGCTCAATTAAACTACAGATCGACGGCAATGTCTTGGCTCCTCACGATAAACTTTTCATCAAGGGTGAATACTGGCTCGCCCTCCATCAGGTCAACAATTTGATCGTCGACGGTCAAGGGGGGTTCGACGGCCAAGGCTCTTCCGCTTGGGGTTATAGAGGTCACGACCGTCCAATCACATTAAAATTTACTGCAATCAACAATGCGACGATAAGGAACATCAAGTCTGTGGACAGTAAATTCTTCCATTTTGCAGTGCACGAAAGCGAAAACATGACGTTTGATAATGTCACACTCATAGCTCCAAAAAACAGTTCCAACACGGATGGAATCCACATCTCCAAATCAACTAACATCAACATCTTCAACTCAGTGATGAGCACAGGAGACGATTGCATCTCCATGGGTCATGGAAGCCAAAACATCAATATAACCAATGTTCAATGTGGTCCAGGCCACGGAATCAGCATCGGAAGCCTTGGCCAAGATCCGAAAGAATTAAATGTGACCAAAATCACCGTAAGACACTCCATATTCAAAAATACCAAAAATGGTGTGAGGATCAAATCATGGTCCACCCCAAATCCAAGTATAGTTACCAAAGTTTCATTCATCCATCTTCAAATGGAGAATGTCGAAAATCCTATCATCATCGATCAAAACTATTGCCCTCATTGCTCTAGCAATCATCAACATCAACTGGTATAA

Coding sequence (CDS)

ATGGCATGGGAAAAAGGTTGTGGATATGATGGTTCTGCAGTGGTTTTAATACCACAAGGCACCTTCTTGGTTTTCCCAATGAAACTTGAAGGTCCTTGCCATGGCTCAATTAAACTACAGATCGACGGCAATGTCTTGGCTCCTCACGATAAACTTTTCATCAAGGGTGAATACTGGCTCGCCCTCCATCAGGTCAACAATTTGATCGTCGACGGTCAAGGGGGGTTCGACGGCCAAGGCTCTTCCGCTTGGGGTTATAGAGGTCACGACCGTCCAATCACATTAAAATTTACTGCAATCAACAATGCGACGATAAGGAACATCAAGTCTGTGGACAGTAAATTCTTCCATTTTGCAGTGCACGAAAGCGAAAACATGACGTTTGATAATGTCACACTCATAGCTCCAAAAAACAGTTCCAACACGGATGGAATCCACATCTCCAAATCAACTAACATCAACATCTTCAACTCAGTGATGAGCACAGGAGACGATTGCATCTCCATGGGTCATGGAAGCCAAAACATCAATATAACCAATGTTCAATGTGGTCCAGGCCACGGAATCAGCATCGGAAGCCTTGGCCAAGATCCGAAAGAATTAAATGTGACCAAAATCACCGTAAGACACTCCATATTCAAAAATACCAAAAATGGTGTGAGGATCAAATCATGGTCCACCCCAAATCCAAGTATAGTTACCAAAGTTTCATTCATCCATCTTCAAATGGAGAATGTCGAAAATCCTATCATCATCGATCAAAACTATTGCCCTCATTGCTCTAGCAATCATCAACATCAACTGGTATAA

Protein sequence

MAWEKGCGYDGSAVVLIPQGTFLVFPMKLEGPCHGSIKLQIDGNVLAPHDKLFIKGEYWLALHQVNNLIVDGQGGFDGQGSSAWGYRGHDRPITLKFTAINNATIRNIKSVDSKFFHFAVHESENMTFDNVTLIAPKNSSNTDGIHISKSTNINIFNSVMSTGDDCISMGHGSQNINITNVQCGPGHGISIGSLGQDPKELNVTKITVRHSIFKNTKNGVRIKSWSTPNPSIVTKVSFIHLQMENVENPIIIDQNYCPHCSSNHQHQLV

Homology

[Close]

BLAST of Lsi04G006260 vs. ExPASy Swiss-Prot
Match: Q6H9K0 (Exopolygalacturonase (Fragment) OS=Platanus acerifolia OX=140101 GN=plaa2 PE=1 SV=1)

HSP 1 Score: 246.5 bits (628), Expect = 3.4e-64
Identity = 122/272 (44.85%), Postives = 169/272 (62.13%), Query Frame = 0


Query: 2   AWEKGCGYDGSAVVLIPQGTFLVFPMKLEGPCHGS-IKLQIDGNVLAPHDKLFIKGEYWL 61
           AW+  C   G + VLIP+G + +  + ++GPC GS I  QIDG V AP D    K + W+
Sbjct: 30  AWKAACASQGPSTVLIPKGNYNMGEVAMQGPCKGSKIGFQIDGVVKAPADPSKFKSDGWV 89

Query: 62  ALHQVNNLIVDGQGGFDGQGSSAWGYRGHDR-------PITLKFTAINNATIRNIKSVDS 121
           + ++++ L V G G  DGQG +AW     D+        + L+F  + +A +R+I S++S
Sbjct: 90  SFYRIDGLTVSGTGTLDGQGQTAWAKNNCDKNPNCKHAAMNLRFDFLKHAMVRDITSLNS 149

Query: 122 KFFHFAVHESENMTFDNVTLIAPKNSSNTDGIHISKSTNINIFNSVMSTGDDCISMGHGS 181
           K FH  V E E++TF +VT+ AP  S NTDGIH+  S  + I N+ ++TGDDCIS+G GS
Sbjct: 150 KMFHINVLECEDITFQHVTVTAPGTSINTDGIHVGISKGVTITNTKIATGDDCISIGPGS 209

Query: 182 QNINITNVQCGPGHGISIGSLGQDPKELNVTKITVRHSIFKNTKNGVRIKSWSTPNPSIV 241
           QN+ IT V CGPGHGISIGSLG+   E  V  ITV+   F  T NGVR+K+W    P   
Sbjct: 210 QNVTITQVNCGPGHGISIGSLGRYNNEKEVRGITVKGCTFSGTMNGVRVKTWPNSPPGAA 269

Query: 242 TKVSFIHLQMENVENPIIIDQNYCPHCSSNHQ 266
           T ++F  L M NV+NP+I+DQ YCP+   + Q
Sbjct: 270 TDLTFQDLTMNNVQNPVILDQEYCPYGQCSRQ 301

[Close]

BLAST of Lsi04G006260 vs. ExPASy Swiss-Prot
Match: Q05967 (Polygalacturonase OS=Nicotiana tabacum OX=4097 GN=PG1 PE=2 SV=1)

HSP 1 Score: 238.4 bits (607), Expect = 9.3e-62
Identity = 114/266 (42.86%), Postives = 171/266 (64.29%), Query Frame = 0


Query: 2   AWEKGCGYDGSAVVLIPQGTFLVFPMKLEGPCHGSIKLQIDGNVLAPHDKLFIKGEYWLA 61
           A+++ C     + ++IP+GTF +  +KLEGPC   ++LQI   + AP D   +K   WL 
Sbjct: 47  AFKEACQSTSPSTIVIPKGTFTMNQVKLEGPCKSPLELQIQATLKAPSDPSQLKVGEWLT 106

Query: 62  LHQVNNLIVDGQGGFDGQGSSAWGYRGHDR----PITLKFTAINNATIRNIKSVDSKFFH 121
           +++++   + G G  DGQ ++AW  +   +    P  L F ++ N+TI++I ++DSK FH
Sbjct: 107 VNKLDQFTMSGGGILDGQAAAAWECKQSKKCNKLPNNLSFNSLTNSTIKDITTLDSKSFH 166

Query: 122 FAVHESENMTFDNVTLIAPKNSSNTDGIHISKSTNINIFNSVMSTGDDCISMGHGSQNIN 181
             V++ +N+TF    + AP NS NTDGIH+S+S+++NI +S  STGDDCIS+G  ++ + 
Sbjct: 167 VNVNQCKNLTFIRFNVSAPANSPNTDGIHVSRSSSVNITDSNFSTGDDCISVGDETEQLY 226

Query: 182 ITNVQCGPGHGISIGSLGQDPKELNVTKITVRHSIFKNTKNGVRIKSWSTPNPSIVTKVS 241
           IT V CGPGHGIS+GSLG +P E  V  + VR+  F NT NGVRIK+W   +P +V  V 
Sbjct: 227 ITRVTCGPGHGISVGSLGGNPDEKPVVGVFVRNCTFTNTDNGVRIKTWPASHPGVVNDVH 286

Query: 242 FIHLQMENVENPIIIDQNYCPHCSSN 264
           F  + ++NV NP++IDQ YCP    N
Sbjct: 287 FEDIIVQNVSNPVVIDQVYCPFNKCN 312

[Close]

BLAST of Lsi04G006260 vs. ExPASy Swiss-Prot
Match: P24548 (Exopolygalacturonase (Fragment) OS=Oenothera organensis OX=3945 PE=2 SV=1)

HSP 1 Score: 233.8 bits (595), Expect = 2.3e-60
Identity = 115/266 (43.23%), Postives = 166/266 (62.41%), Query Frame = 0


Query: 2   AWEKGCGYDGSAVVLIPQGTFLVFPMKLEGPCHGSIKLQIDGNVLAPHDKLFIKGEYWLA 61
           AW++ C     + +L+P+G F V  + LEGPC  SI LQ+ G + AP D   IKG  W+ 
Sbjct: 9   AWKEACASASPSTILVPKGNFAVGLITLEGPCKSSIGLQLQGTLKAPADPSKIKGLGWIN 68

Query: 62  LHQVNNLIVDGQGGFDGQGSSAW--------GYRGHDRPITLKFTAINNATIRNIKSVDS 121
           L++++ L + G G FDGQG SAW        G       + L+  A+ N+ +R++ ++DS
Sbjct: 69  LNKIDLLTIFGGGVFDGQGKSAWVQNDCHKNGPICKTLSMNLRLYAVTNSILRDVTTLDS 128

Query: 122 KFFHFAVHESENMTFDNVTLIAPKNSSNTDGIHISKSTNINIFNSVMSTGDDCISMGHGS 181
           K FH  V   +N+TF+   + A + S NTDGIHI +S  +NI N+ + TGDDCIS+G GS
Sbjct: 129 KNFHVNVIGCKNLTFERFKISAAETSINTDGIHIGRSDGVNIINTEIKTGDDCISLGDGS 188

Query: 182 QNINITNVQCGPGHGISIGSLGQDPKELNVTKITVRHSIFKNTKNGVRIKSWSTPNPSIV 241
           +NINITN+ CGPGHGIS+GSLG+   E +V  I V++     ++NGVRIK+W    P   
Sbjct: 189 KNINITNITCGPGHGISVGSLGRYKNEESVVGIYVKNCTITGSQNGVRIKTWPKSEPGEA 248

Query: 242 TKVSFIHLQMENVENPIIIDQNYCPH 260
           +++ F  + M +V  PI+IDQ YCP+
Sbjct: 249 SEMHFQDITMNSVGTPILIDQGYCPY 274

[Close]

BLAST of Lsi04G006260 vs. ExPASy Swiss-Prot
Match: Q40312 (Polygalacturonase OS=Medicago sativa OX=3879 PE=2 SV=1)

HSP 1 Score: 230.3 bits (586), Expect = 2.5e-59
Identity = 118/282 (41.84%), Postives = 171/282 (60.64%), Query Frame = 0


Query: 2   AWEKGCGYDGSAVVLIPQGTFLVFPMKLEGPCHGSIKLQIDGNVLAPHDKLFIKG-EYWL 61
           AW + C    +A ++IP GT+ +  ++L+GPC   I+LQ+DG + AP D   IKG E W 
Sbjct: 45  AWNEACASTTAAKIVIPAGTYQLNGIELKGPCKAPIELQVDGTIQAPADPSVIKGTEQWF 104

Query: 62  ALHQVNNLIVDGQGGFDGQG---------SSAWGYRGHDRPITLK--FTAINNATIRNIK 121
               +++L + G+G FDGQG         +SAW  +  +  + +   F  +NN+ +R + 
Sbjct: 105 KFLYMDHLTLSGKGVFDGQGATVYKKAAPASAWSGKNSNSKVFMNFGFNFVNNSIVRGVT 164

Query: 122 SVDSKFFHFAVHESENMTFDNVTLIAPKNSSNTDGIHISKSTNINIFNSVMSTGDDCISM 181
           S DSK FH  V   +N+TFD  T+ AP +S NTDGIH+ KST++ I N+ + TGDDC+S+
Sbjct: 165 SKDSKNFHVMVFGCKNITFDGFTITAPGDSPNTDGIHMGKSTDVKILNTNIGTGDDCVSI 224

Query: 182 GHGSQNINITNVQCGPGHGISIGSLGQDPKELNVTKITVRHSIFKNTKNGVRIKSW-STP 241
           G GS+ I +  V CGPGHG+S+GSLG+   E NV  ITV++     T NGVRIK+W   P
Sbjct: 225 GDGSKQITVQGVNCGPGHGLSVGSLGKFTTEENVEGITVKNCTLTATDNGVRIKTWPDAP 284

Query: 242 NPSIVTKVSFIHLQMENVENPIIIDQNYCP--HCSSNHQHQL 269
               V+ + F  + M NV+NP+IIDQ Y P   CS  +  ++
Sbjct: 285 GTITVSDIHFEDITMTNVKNPVIIDQEYYPWNQCSKKNPSKI 326

[Close]

BLAST of Lsi04G006260 vs. ExPASy Swiss-Prot
Match: P35338 (Exopolygalacturonase OS=Zea mays OX=4577 GN=PG9 PE=2 SV=1)

HSP 1 Score: 224.9 bits (572), Expect = 1.1e-57
Identity = 113/273 (41.39%), Postives = 166/273 (60.81%), Query Frame = 0


Query: 2   AWEKGCGYDGSAVVLIPQGTFLVFPMKLEGPCHGSIKLQIDGNVLAPHD-KLFIKGEYWL 61
           AW   CG  G   +LIP+G FLV  +   GPC G + +Q+DGN+LA  D   + +   W+
Sbjct: 63  AWASACGGTGKQTILIPKGDFLVGQLNFTGPCKGDVTIQVDGNLLATTDLSQYKEHGNWI 122

Query: 62  ALHQVNNLIVDGQGGFDGQGSSAWG-------YRGHDRPITLKFTAINNATIRNIKSVDS 121
            + +V+NL++ G+G  DGQG + W        Y     P +L    +NN  +  +  ++S
Sbjct: 123 EILRVDNLVITGKGNLDGQGPAVWSKNSCTKKYDCKILPNSLVMDFVNNGEVSGVTLLNS 182

Query: 122 KFFHFAVHESENMTFDNVTLIAPKNSSNTDGIHISKSTNINIFNSVMSTGDDCISMGHGS 181
           KFFH  +++ +NM   +VT+ AP +S NTDGIH+  S+ I I N+V+  GDDCIS+G G+
Sbjct: 183 KFFHMNMYQCKNMLIKDVTVTAPGDSPNTDGIHMGDSSGITITNTVIGVGDDCISIGPGT 242

Query: 182 QNINITNVQCGPGHGISIGSLGQDPKELNVTKITVRHSIFKNTKNGVRIKSW-STPNPSI 241
             +NIT V CGPGHGISIGSLG+   E +VT I V+    K T  GVRIK++    +   
Sbjct: 243 SKVNITGVTCGPGHGISIGSLGRYKDEKDVTDINVKDCTLKKTMFGVRIKAYEDAASVLT 302

Query: 242 VTKVSFIHLQMENVENPIIIDQNYCPH--CSSN 264
           V+K+ + +++ME+  NPI ID  YCP+  C++N
Sbjct: 303 VSKIHYENIKMEDSANPIFIDMKYCPNKLCTAN 335

[Close]

BLAST of Lsi04G006260 vs. ExPASy TrEMBL
Match: A0A0A0L7H9 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G063610 PE=3 SV=1)

HSP 1 Score: 335.5 bits (859), Expect = 2.1e-88
Identity = 155/258 (60.08%), Postives = 201/258 (77.91%), Query Frame = 0


Query: 2   AWEKGCGYDGSAVVLIP-QGTFLVFPMKLEGPCHGSIKLQIDGNVLAPHDKLFIKGEYWL 61
           AW + C  +G  +VLIP +G FLV P+ L+GPCHG I++Q+DG +LAP D+ F  G+YWL
Sbjct: 20  AWSEACTNNGGGIVLIPAKGRFLVLPLLLQGPCHGFIRIQLDGELLAPLDEHFATGDYWL 79

Query: 62  ALHQVNNLIVDGQGGFDGQGSSAWGYRGHDRPITLKFTAINNATIRNIKSVDSKFFHFAV 121
           ++ QVNNL +DG G  DG+GS+AW    H+RP++++   INNA I NIKS +SK FHFA+
Sbjct: 80  SIDQVNNLFIDGLGSLDGRGSTAWPSTSHNRPVSMRLNGINNARITNIKSYNSKLFHFAM 139

Query: 122 HESENMTFDNVTLIAPKNSSNTDGIHISKSTNINIFNSVMSTGDDCISMGHGSQNINITN 181
           H   ++TFD+VT+IAP NS NTDGIHIS S+ INI +S + TGDDCIS+G GS+ INITN
Sbjct: 140 HGCRDVTFDHVTVIAPANSPNTDGIHISSSSGINIMHSTIGTGDDCISLGPGSKFINITN 199

Query: 182 VQCGPGHGISIGSLGQDPKELNVTKITVRHSIFKNTKNGVRIKSWSTPNPSIVTKVSFIH 241
           +QCGPGHGISIGSLG+ P E +V ++TVR S F  T NGVRIK+WS+   S+V+KV+F++
Sbjct: 200 IQCGPGHGISIGSLGKYPNEEDVFEVTVRDSTFIGTSNGVRIKTWSSSYSSMVSKVTFLN 259

Query: 242 LQMENVENPIIIDQNYCP 259
           LQM NV+NPIIIDQNYCP
Sbjct: 260 LQMNNVKNPIIIDQNYCP 277

[Close]

BLAST of Lsi04G006260 vs. ExPASy TrEMBL
Match: A0A5D3BB58 (Exopolygalacturonase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold546G001870 PE=3 SV=1)

HSP 1 Score: 325.1 bits (832), Expect = 2.8e-85
Identity = 159/270 (58.89%), Postives = 198/270 (73.33%), Query Frame = 0


Query: 2   AWEKGCGYDGSAVVLIP-QGTFLVFPMKLEGPCHGSIKLQIDGNVLAPHDKLFIKGEYWL 61
           AW   C  +G  VVLIP QG FLV P+ L+GPCHG I++Q+DG +LAP DK F  G+YWL
Sbjct: 6   AWSGACENNGGGVVLIPAQGRFLVLPLLLQGPCHGVIRIQLDGELLAPSDKQFATGDYWL 65

Query: 62  ALHQVNNLIVDGQGGFDGQGSSAWGYRGHDRPI------TLKFTAINNATIRNIKSVDSK 121
           ++  VNNL +DG G   G GSSAW     +RPI      ++K   IN A+I+NI S DSK
Sbjct: 66  SIQNVNNLFIDGLGRLTGSGSSAWPSHSANRPIVRFHLSSMKLNNINTASIKNISSYDSK 125

Query: 122 FFHFAVHESENMTFDNVTLIAPKNSSNTDGIHISKSTNINIFNSVMSTGDDCISMGHGSQ 181
            FHFAVH S ++TFD+VT++AP +S NTDGIHIS S+ INI +S + TGDDCIS+G GS+
Sbjct: 126 LFHFAVHGSRDVTFDHVTVVAPGDSPNTDGIHISSSSGINIMHSTIGTGDDCISLGPGSK 185

Query: 182 NINITNVQCGPGHGISIGSLGQDPKELNVTKITVRHSIFKNTKNGVRIKSWSTPNPSIVT 241
           +INITNV CGPGHGISIGSLG+ P E NV ++TVR S F  T NG RIKSWS+   S+V+
Sbjct: 186 SINITNVYCGPGHGISIGSLGKYPNEENVFEVTVRDSTFVGTTNGARIKSWSSSYSSMVS 245

Query: 242 KVSFIHLQMENVENPIIIDQNYCP-HCSSN 264
           KV+F++LQM NV+NPIIIDQ+YCP  C  N
Sbjct: 246 KVTFVNLQMNNVKNPIIIDQSYCPDSCGPN 275

[Close]

BLAST of Lsi04G006260 vs. ExPASy TrEMBL
Match: A0A1S4DX91 (exopolygalacturonase-like OS=Cucumis melo OX=3656 GN=LOC103491147 PE=3 SV=1)

HSP 1 Score: 324.3 bits (830), Expect = 4.7e-85
Identity = 157/268 (58.58%), Postives = 202/268 (75.37%), Query Frame = 0


Query: 2   AWEKGCGYDGSAVVLIPQ-GTFLVFPMKLEGPCHGSIKLQIDGNVLAPHDKLFIKGEYWL 61
           AW + C  +G  VVLIP  G FLV P+ L+GPCHG I +Q+DG +LAP ++    G+YWL
Sbjct: 20  AWSEACTNNGGGVVLIPALGRFLVLPLLLQGPCHGFIHIQLDGELLAPLNE--NTGDYWL 79

Query: 62  ALHQVNNLIVDGQGGFDGQGSSAWGYRGHDRPITLKFTAINNATIRNIKSVDSKFFHFAV 121
           ++ +VNNL +DG G  +G+GS+AW    H+RPI++K T INNA I NIKS +SK FHFAV
Sbjct: 80  SIDKVNNLFIDGLGRLNGRGSTAWPSASHNRPISMKLTGINNARISNIKSYNSKLFHFAV 139

Query: 122 HESENMTFDNVTLIAPKNSSNTDGIHISKSTNINIFNSVMSTGDDCISMGHGSQNINITN 181
           H   ++TFD+VT+IAP NS NTDGIH+S S+ INI +S + TGDDCIS+G GS+ INITN
Sbjct: 140 HGCRDVTFDHVTVIAPANSPNTDGIHVSLSSGINIMHSTIGTGDDCISLGPGSKFINITN 199

Query: 182 VQCGPGHGISIGSLGQDPKELNVTKITVRHSIFKNTKNGVRIKSWSTPNPSIVTKVSFIH 241
           VQCGPGHGISIGSLG+ P E +V ++TVR S F  T NG RIK+WS+   ++V+KV+F++
Sbjct: 200 VQCGPGHGISIGSLGKYPNEEDVFEVTVRDSTFLGTTNGARIKTWSSSYSNMVSKVTFLN 259

Query: 242 LQMENVENPIIIDQNYCPH-CSSNHQHQ 268
           LQM NV+NPIIIDQ+YCPH C  N   Q
Sbjct: 260 LQMNNVKNPIIIDQSYCPHSCGPNEMSQ 285

[Close]

BLAST of Lsi04G006260 vs. ExPASy TrEMBL
Match: A0A5D3B9J1 (Exopolygalacturonase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold546G001860 PE=3 SV=1)

HSP 1 Score: 323.9 bits (829), Expect = 6.2e-85
Identity = 156/268 (58.21%), Postives = 202/268 (75.37%), Query Frame = 0


Query: 2   AWEKGCGYDGSAVVLIPQ-GTFLVFPMKLEGPCHGSIKLQIDGNVLAPHDKLFIKGEYWL 61
           AW + C  +G  VVLIP  G FLV P+ L+GPCHG I +Q+DG +LAP ++    G+YWL
Sbjct: 20  AWSEACTNNGGGVVLIPALGRFLVLPLLLQGPCHGFIHIQLDGELLAPLNE--NTGDYWL 79

Query: 62  ALHQVNNLIVDGQGGFDGQGSSAWGYRGHDRPITLKFTAINNATIRNIKSVDSKFFHFAV 121
           ++ +VNNL +DG G  +G+GS+AW    H+RPI++K T INNA I NIKS +SK FHFAV
Sbjct: 80  SIDKVNNLFIDGLGRLNGRGSTAWPSASHNRPISMKLTGINNARISNIKSYNSKLFHFAV 139

Query: 122 HESENMTFDNVTLIAPKNSSNTDGIHISKSTNINIFNSVMSTGDDCISMGHGSQNINITN 181
           H   ++TFD+VT+IAP NS NTDGIH+S S+ INI +S + TGDDCIS+G GS+ INITN
Sbjct: 140 HGCRDVTFDHVTVIAPANSPNTDGIHVSLSSGINIMHSTIGTGDDCISLGPGSKFINITN 199

Query: 182 VQCGPGHGISIGSLGQDPKELNVTKITVRHSIFKNTKNGVRIKSWSTPNPSIVTKVSFIH 241
           VQCGPGHGISIGSLG+ P E +V ++TVR S F  T NG RIK+WS+   ++V+K++F++
Sbjct: 200 VQCGPGHGISIGSLGKYPNEEDVFEVTVRDSTFLGTTNGARIKTWSSSYSNMVSKITFLN 259

Query: 242 LQMENVENPIIIDQNYCPH-CSSNHQHQ 268
           LQM NV+NPIIIDQ+YCPH C  N   Q
Sbjct: 260 LQMNNVKNPIIIDQSYCPHSCGPNEMSQ 285

[Close]

BLAST of Lsi04G006260 vs. ExPASy TrEMBL
Match: A0A6J1F153 (exopolygalacturonase-like OS=Cucurbita moschata OX=3662 GN=LOC111438612 PE=3 SV=1)

HSP 1 Score: 315.8 bits (808), Expect = 1.7e-82
Identity = 146/262 (55.73%), Postives = 197/262 (75.19%), Query Frame = 0


Query: 2   AWEKGCGYDGSAVVLIPQGTFLVFPMKLEGPCHGSIKLQIDGNVLAPHDKLFIKGEYWLA 61
           AW + CG++G  VVL+P G FL+ P+ LEGPC  SI L I G +LAP  K F  G YWL+
Sbjct: 45  AWREACGHNGHGVVLVPAGRFLILPLVLEGPCKVSIGLLIHGELLAPVSKNFATGGYWLS 104

Query: 62  LHQVNNLIVDGQGGFDGQGSSAWGYRGHDRPITLKFTAINNATIRNIKSVDSKFFHFAVH 121
           + +V+NL VDG G  +G GSSAW     +RP+++K +++N A I  I S +SKFFHF VH
Sbjct: 105 ISKVDNLFVDGSGSLNGGGSSAWPSDTSNRPVSMKLSSVNTARISKITSSNSKFFHFGVH 164

Query: 122 ESENMTFDNVTLIAPKNSSNTDGIHISKSTNINIFNSVMSTGDDCISMGHGSQNINITNV 181
              ++TFD+VT+IAP +S NTDGIH+SKS+ INI +SV+ TGDDCIS+G GS+ INIT++
Sbjct: 165 GCRDVTFDHVTVIAPADSPNTDGIHVSKSSGINIMHSVIRTGDDCISLGPGSKFINITDI 224

Query: 182 QCGPGHGISIGSLGQDPKELNVTKITVRHSIFKNTKNGVRIKSWSTPNPSIVTKVSFIHL 241
           QCGPGHGISIGSLG+ P E +V+++T+R S F  T NGVRIK+WS+   S V+K++F++L
Sbjct: 225 QCGPGHGISIGSLGKYPNEEDVSEVTIRDSTFVGTDNGVRIKTWSSSFSSFVSKIAFLNL 284

Query: 242 QMENVENPIIIDQNYCPHCSSN 264
           +M++V+NPIIIDQ YCP C  +
Sbjct: 285 EMKDVKNPIIIDQKYCPDCKED 306

[Close]

BLAST of Lsi04G006260 vs. NCBI nr
Match: XP_038904214.1 (exopolygalacturonase-like [Benincasa hispida])

HSP 1 Score: 337.8 bits (865), Expect = 8.5e-89
Identity = 162/269 (60.22%), Postives = 205/269 (76.21%), Query Frame = 0


Query: 2   AWEKGCGYDGSAVVLIP-QGTFLVFPMKLEGPCHGSIKLQIDGNVLAPHDKLFIKGEYWL 61
           AW + CG  G  VVLIP QG FLV P+ L+GPCHG I +Q+DG +LAP +K F  G+YWL
Sbjct: 53  AWSEACGNSGGGVVLIPAQGRFLVLPLLLQGPCHGFIHIQLDGELLAPVNKQFATGDYWL 112

Query: 62  ALHQVNNLIVDGQGGFDGQGSSAWGYRGHDRPITLKFTAINNATIRNIKSVDSKFFHFAV 121
           +++QVNNL +DG G  +G+GS+AW Y    RP+++K T IN A IRNIKS +SK FHFAV
Sbjct: 113 SINQVNNLFIDGLGRLNGRGSTAWPYSSASRPVSMKLTNINTARIRNIKSYNSKLFHFAV 172

Query: 122 HESENMTFDNVTLIAPKNSSNTDGIHISKSTNINIFNSVMSTGDDCISMGHGSQNINITN 181
           H   N+ FD+VT+IAP +S NTDGIHIS S+ INI +SV+STGDDCIS+G GS+ INITN
Sbjct: 173 HGCHNVMFDHVTVIAPTDSPNTDGIHISSSSGINIMHSVISTGDDCISLGPGSKFINITN 232

Query: 182 VQCGPGHGISIGSLGQDPKELNVTKITVRHSIFKNTKNGVRIKSWSTPNPSIVTKVSFIH 241
           +QCGPGHGISIGSLG+ P E +V ++T+R SIF  T NGVRIKSWS+   S+V+KVS+ +
Sbjct: 233 IQCGPGHGISIGSLGKYPNEEDVFEVTIRDSIFIGTSNGVRIKSWSSSYSSLVSKVSYRN 292

Query: 242 LQMENVENPIIIDQNYCP-HCSSNHQHQL 269
           LQM NV+NPI+IDQ YCP  C  N   ++
Sbjct: 293 LQMNNVKNPILIDQMYCPDSCGPNEMSRV 321

[Close]

BLAST of Lsi04G006260 vs. NCBI nr
Match: XP_031738767.1 (exopolygalacturonase [Cucumis sativus])

HSP 1 Score: 335.5 bits (859), Expect = 4.2e-88
Identity = 155/258 (60.08%), Postives = 201/258 (77.91%), Query Frame = 0


Query: 2   AWEKGCGYDGSAVVLIP-QGTFLVFPMKLEGPCHGSIKLQIDGNVLAPHDKLFIKGEYWL 61
           AW + C  +G  +VLIP +G FLV P+ L+GPCHG I++Q+DG +LAP D+ F  G+YWL
Sbjct: 20  AWSEACTNNGGGIVLIPAKGRFLVLPLLLQGPCHGFIRIQLDGELLAPLDEHFATGDYWL 79

Query: 62  ALHQVNNLIVDGQGGFDGQGSSAWGYRGHDRPITLKFTAINNATIRNIKSVDSKFFHFAV 121
           ++ QVNNL +DG G  DG+GS+AW    H+RP++++   INNA I NIKS +SK FHFA+
Sbjct: 80  SIDQVNNLFIDGLGSLDGRGSTAWPSTSHNRPVSMRLNGINNARITNIKSYNSKLFHFAM 139

Query: 122 HESENMTFDNVTLIAPKNSSNTDGIHISKSTNINIFNSVMSTGDDCISMGHGSQNINITN 181
           H   ++TFD+VT+IAP NS NTDGIHIS S+ INI +S + TGDDCIS+G GS+ INITN
Sbjct: 140 HGCRDVTFDHVTVIAPANSPNTDGIHISSSSGINIMHSTIGTGDDCISLGPGSKFINITN 199

Query: 182 VQCGPGHGISIGSLGQDPKELNVTKITVRHSIFKNTKNGVRIKSWSTPNPSIVTKVSFIH 241
           +QCGPGHGISIGSLG+ P E +V ++TVR S F  T NGVRIK+WS+   S+V+KV+F++
Sbjct: 200 IQCGPGHGISIGSLGKYPNEEDVFEVTVRDSTFIGTSNGVRIKTWSSSYSSMVSKVTFLN 259

Query: 242 LQMENVENPIIIDQNYCP 259
           LQM NV+NPIIIDQNYCP
Sbjct: 260 LQMNNVKNPIIIDQNYCP 277

[Close]

BLAST of Lsi04G006260 vs. NCBI nr
Match: XP_004149596.1 (exopolygalacturonase [Cucumis sativus])

HSP 1 Score: 335.1 bits (858), Expect = 5.5e-88
Identity = 159/268 (59.33%), Postives = 203/268 (75.75%), Query Frame = 0


Query: 2   AWEKGCGYDGSAVVLIP-QGTFLVFPMKLEGPCHGSIKLQIDGNVLAPHDKLFIKGEYWL 61
           AW   CG +G  VVLIP QG FLV P+ L+GPCHG I++Q+DG +LA  DK F  G++WL
Sbjct: 10  AWSGACGNNGGGVVLIPAQGKFLVLPLLLQGPCHGVIRIQLDGELLASSDKQFAIGDHWL 69

Query: 62  ALHQVNNLIVDGQGGFDGQGSSAWGYRGHDRPITLKFTAINNATIRNIKSVDSKFFHFAV 121
           ++  VNNL +DG G   G G+SAW     +RPI++KF  +N A+I+NI S DSKFFHFAV
Sbjct: 70  SIENVNNLFIDGLGRLTGSGASAWPSNSLNRPISMKFNNVNTASIKNISSFDSKFFHFAV 129

Query: 122 HESENMTFDNVTLIAPKNSSNTDGIHISKSTNINIFNSVMSTGDDCISMGHGSQNINITN 181
           H S ++TFDNVT++AP NS NTDGIHIS S+ INI +S + TGDDCIS+G GS+ INI+N
Sbjct: 130 HASRDVTFDNVTVVAPANSPNTDGIHISTSSGINIMHSTIGTGDDCISLGPGSKMINISN 189

Query: 182 VQCGPGHGISIGSLGQDPKELNVTKITVRHSIFKNTKNGVRIKSWSTPNPSIVTKVSFIH 241
           V CGPGHGISIGSLG++P E +V  +TVR S F  T NG RIKSWS+P  S+V+KV+F++
Sbjct: 190 VHCGPGHGISIGSLGKNPNEEDVFDVTVRDSTFIGTSNGARIKSWSSPYSSMVSKVTFLN 249

Query: 242 LQMENVENPIIIDQNYCP-HCSSNHQHQ 268
           LQM NV+NPIIIDQ+YCP  C+ N  ++
Sbjct: 250 LQMNNVKNPIIIDQSYCPDSCAPNKMNK 277

[Close]

BLAST of Lsi04G006260 vs. NCBI nr
Match: XP_038904218.1 (exopolygalacturonase-like [Benincasa hispida])

HSP 1 Score: 326.2 bits (835), Expect = 2.6e-85
Identity = 155/264 (58.71%), Postives = 200/264 (75.76%), Query Frame = 0


Query: 2   AWEKGCGYDGSAVVLIPQ-GTFLVFPMKLEGPCHGSIKLQIDGNVLAPHDKLFIKGEYWL 61
           AW + CG +G  VVLIP  G FLV P+ L+GPCHG I++Q DG +LAP D  F  G YWL
Sbjct: 65  AWSEACGNNGGGVVLIPALGRFLVLPLLLQGPCHGFIRIQFDGELLAPTDNHFATGNYWL 124

Query: 62  ALHQVNNLIVDGQGGFDGQGSSAWGYRGHDRPITLKFTAINNATIRNIKSVDSKFFHFAV 121
           +++QVNNL +DG G  +G+GS+AW Y+  +RPI++K   IN A IRNIKS +SK FHFA+
Sbjct: 125 SINQVNNLFIDGLGRLNGRGSTAWYYKSANRPISMKLNNINTARIRNIKSYNSKLFHFAM 184

Query: 122 HESENMTFDNVTLIAPKNSSNTDGIHISKSTNINIFNSVMSTGDDCISMGHGSQNINITN 181
           H   ++TFD+VT+IAP NS NTDGIHIS S+ INI +S++ TGDDCIS+G GS+ INITN
Sbjct: 185 HGCHDVTFDHVTMIAPANSPNTDGIHISSSSGINIMHSMIGTGDDCISLGPGSKFINITN 244

Query: 182 VQCGPGHGISIGSLGQDPKELNVTKITVRHSIFKNTKNGVRIKSWSTPNPSIVTKVSFIH 241
           V+CGPGHGISIGSLG+ P E ++ ++TVR S F  T NGVRIKSW +   S+V+KV++++
Sbjct: 245 VRCGPGHGISIGSLGKYPNEEDIFEVTVRDSTFIGTSNGVRIKSWLSSYSSMVSKVTYMN 304

Query: 242 LQMENVENPIIIDQNYCP-HCSSN 264
           LQM NV+NP+ IDQ YCP  C  N
Sbjct: 305 LQMNNVKNPVPIDQMYCPDSCGPN 328

[Close]

BLAST of Lsi04G006260 vs. NCBI nr
Match: KAA0065794.1 (exopolygalacturonase-like [Cucumis melo var. makuwa] >TYJ96533.1 exopolygalacturonase-like [Cucumis melo var. makuwa])

HSP 1 Score: 325.1 bits (832), Expect = 5.7e-85
Identity = 159/270 (58.89%), Postives = 198/270 (73.33%), Query Frame = 0


Query: 2   AWEKGCGYDGSAVVLIP-QGTFLVFPMKLEGPCHGSIKLQIDGNVLAPHDKLFIKGEYWL 61
           AW   C  +G  VVLIP QG FLV P+ L+GPCHG I++Q+DG +LAP DK F  G+YWL
Sbjct: 6   AWSGACENNGGGVVLIPAQGRFLVLPLLLQGPCHGVIRIQLDGELLAPSDKQFATGDYWL 65

Query: 62  ALHQVNNLIVDGQGGFDGQGSSAWGYRGHDRPI------TLKFTAINNATIRNIKSVDSK 121
           ++  VNNL +DG G   G GSSAW     +RPI      ++K   IN A+I+NI S DSK
Sbjct: 66  SIQNVNNLFIDGLGRLTGSGSSAWPSHSANRPIVRFHLSSMKLNNINTASIKNISSYDSK 125

Query: 122 FFHFAVHESENMTFDNVTLIAPKNSSNTDGIHISKSTNINIFNSVMSTGDDCISMGHGSQ 181
            FHFAVH S ++TFD+VT++AP +S NTDGIHIS S+ INI +S + TGDDCIS+G GS+
Sbjct: 126 LFHFAVHGSRDVTFDHVTVVAPGDSPNTDGIHISSSSGINIMHSTIGTGDDCISLGPGSK 185

Query: 182 NINITNVQCGPGHGISIGSLGQDPKELNVTKITVRHSIFKNTKNGVRIKSWSTPNPSIVT 241
           +INITNV CGPGHGISIGSLG+ P E NV ++TVR S F  T NG RIKSWS+   S+V+
Sbjct: 186 SINITNVYCGPGHGISIGSLGKYPNEENVFEVTVRDSTFVGTTNGARIKSWSSSYSSMVS 245

Query: 242 KVSFIHLQMENVENPIIIDQNYCP-HCSSN 264
           KV+F++LQM NV+NPIIIDQ+YCP  C  N
Sbjct: 246 KVTFVNLQMNNVKNPIIIDQSYCPDSCGPN 275

[Close]

BLAST of Lsi04G006260 vs. TAIR 10
Match: AT1G17150.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 224.6 bits (571), Expect = 9.8e-59
Identity = 118/271 (43.54%), Postives = 168/271 (61.99%), Query Frame = 0


Query: 2   AWEKGCGYDGSAVVLIPQGTFLVFPMKLEGPCHGSIKLQIDGNVLAPHDKLFIKGEYWLA 61
           AW+  C ++G + + IP GTF +  +   GPC G I   IDG +LAP +   IK E W+ 
Sbjct: 55  AWKDACRWNGPSKMYIPLGTFYLGGVTFVGPCDGKISFVIDGTLLAPPNNDDIKKEIWIN 114

Query: 62  LHQVNNLIVDGQGGFDGQGSSAWG----YRGHDRP---ITLKFTAINNATIRNIKSVDSK 121
              +N L V G G  DGQG  +W      + ++ P   I + F  + N+++  I S++SK
Sbjct: 115 FRYINYLTVFGDGTLDGQGKKSWSLIDCQKDNNCPKLAINMGFDFVKNSSMNGITSLNSK 174

Query: 122 FFHFAVHESENMTFDNVTLIAPKNSSNTDGIHISKSTNINIFNSVMSTGDDCISMGHGSQ 181
             HF     ++ +   V +IAP NS NTDGI I+ S+N+ I N+ +STGDDCI+M  G+ 
Sbjct: 175 AGHFNFLSVDHFSITRVNIIAPSNSPNTDGIKIALSSNMQISNTHISTGDDCIAMLSGNT 234

Query: 182 NINITNVQCGPGHGISIGSLGQDPKELNVTKITVRHSIFKNTKNGVRIKSWSTPNPSI-V 241
           N +I NV+CGPGHGISIGSLG++  E NV  + VR+S+F  T NG+RIK+W +   +I +
Sbjct: 235 NFDIYNVKCGPGHGISIGSLGKNKDEKNVNGLMVRNSVFTGTTNGIRIKTWESSASTIRI 294

Query: 242 TKVSFIHLQMENVENPIIIDQNYCPH--CSS 263
             + + +LQM NVENPI IDQ YCP+  CS+
Sbjct: 295 INLVYENLQMINVENPIGIDQKYCPYPPCSN 325

[Close]

BLAST of Lsi04G006260 vs. TAIR 10
Match: AT1G65570.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 222.2 bits (565), Expect = 4.9e-58
Identity = 116/270 (42.96%), Postives = 167/270 (61.85%), Query Frame = 0


Query: 2   AWEKGCGYDGSAVVLIPQGTFLVF-PMKLEGPCHGS--IKLQIDGNVLAPHD-KLFIKGE 61
           AW+  CG + S V+ +P+G +LV   ++ EG    S  I L+IDG ++ P D  L  K E
Sbjct: 53  AWDAACGVEDSVVIYVPKGRYLVSGEVRFEGESCKSREITLRIDGTLIGPQDYSLLGKKE 112

Query: 62  YWLALHQVNNLIVDGQGGFDGQGSSAW-----GYRGHDRPITLKFTAINNATIRNIKSVD 121
            W +   V+N+ V G G FD +GS+ W     GY   +   TL+F   NN  I+ + S++
Sbjct: 113 NWFSFSGVHNVTVLG-GSFDAKGSTLWSCKANGYNCPEGATTLRFMDSNNVKIKGVLSLN 172

Query: 122 SKFFHFAVHESENMTFDNVTLIAPKNSSNTDGIHISKSTNINIFNSVMSTGDDCISMGHG 181
           S+ FH A++   N+  ++V +IAP  S NTDGIHI  ST+I + N+ + TGDDCIS+G G
Sbjct: 173 SQLFHIAINRCRNIKIEDVRIIAPDESPNTDGIHIQLSTDIEVRNASIKTGDDCISIGPG 232

Query: 182 SQNINITNVQCGPGHGISIGSLGQDPKELNVTKITVRHSIFKNTKNGVRIKSWSTPNPSI 241
           ++N+ +  + CGPGHGISIGSL +  +E  V  +TV++++F  T NG+RIKSW   +   
Sbjct: 233 TKNLMVDGITCGPGHGISIGSLAKSIEEQGVENVTVKNAVFVRTDNGLRIKSWPRHSNGF 292

Query: 242 VTKVSFIHLQMENVENPIIIDQNYCPHCSS 263
           V +V F+   M NV  PI+IDQNYCP  SS
Sbjct: 293 VERVRFLGAIMVNVSYPILIDQNYCPGDSS 321

[Close]

BLAST of Lsi04G006260 vs. TAIR 10
Match: AT1G05660.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 222.2 bits (565), Expect = 4.9e-58
Identity = 111/263 (42.21%), Postives = 160/263 (60.84%), Query Frame = 0


Query: 2   AWEKGCGYDGSAVVLIPQGTFLVFPMKLEGPCHGSIKLQIDGNVLAPHD-KLFIKGEYWL 61
           AW+  C    SA V++P+GTFL+  +   GPC   I  Q+ G V+AP D + F    +W+
Sbjct: 50  AWQGACVSASSATVVVPKGTFLLKVITFGGPCKSKITFQVAGTVIAPEDYRTFGNSGFWI 109

Query: 62  ALHQVNNLIVDGQGGFDGQGSSAWGYR--GHDRP---ITLKFTAINNATIRNIKSVDSKF 121
             ++VN   + G G FD + +  W  R  G + P    ++ F +  +  I  +KS++S+ 
Sbjct: 110 LFNKVNRFSLVG-GTFDARANGFWSCRKSGQNCPPGVRSISFNSAKDVIISGVKSMNSQV 169

Query: 122 FHFAVHESENMTFDNVTLIAPKNSSNTDGIHISKSTNINIFNSVMSTGDDCISMGHGSQN 181
            H  ++   N+   NV L+AP NS NTDG H+  ST +    S + TGDDC+++G G++N
Sbjct: 170 THMTLNGCTNVVVRNVKLVAPGNSPNTDGFHVQHSTGVTFTGSTVQTGDDCVAIGPGTRN 229

Query: 182 INITNVQCGPGHGISIGSLGQDPKELNVTKITVRHSIFKNTKNGVRIKSWSTPNPSIVTK 241
           + IT + CGPGHG+SIGSL ++ KE  V  +TV  S+F  ++NGVRIKSW+ P+   V  
Sbjct: 230 LLITKLACGPGHGVSIGSLAKELKEDGVENVTVSSSVFTGSQNGVRIKSWARPSNGFVRT 289

Query: 242 VSFIHLQMENVENPIIIDQNYCP 259
           V F  L M+NVENPIIIDQNYCP
Sbjct: 290 VFFQDLVMKNVENPIIIDQNYCP 311

[Close]

BLAST of Lsi04G006260 vs. TAIR 10
Match: AT3G14040.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 221.9 bits (564), Expect = 6.4e-58
Identity = 108/272 (39.71%), Postives = 164/272 (60.29%), Query Frame = 0


Query: 2   AWEKGCGYDGSAVVLIPQGTFLVFPMKLEGPCHGSIKLQIDGNVLAPHDKLFIKGEY-WL 61
           AW++ C     + + +P+G +LV  ++ +GPC G + L+++GN  AP      K    W+
Sbjct: 93  AWKEACA--AGSTITVPKGEYLVESLEFKGPCKGPVTLELNGNFKAPATVKTTKPHAGWI 152

Query: 62  ALHQVNNLIVDGQGG-FDGQGSSAWGY-------RGHDRPITLKFTAINNATIRNIKSVD 121
               + +  ++G    FDGQGS AW         + +  PI ++FT + N+ I +I S +
Sbjct: 153 DFENLADFTLNGNKAIFDGQGSLAWKANDCAKTGKCNSLPINIRFTGLTNSKINSITSTN 212

Query: 122 SKFFHFAVHESENMTFDNVTLIAPKNSSNTDGIHISKSTNINIFNSVMSTGDDCISMGHG 181
           SK FH  +   +N+T  ++ + AP  S NTDGIHI +S  +N+  + + TGDDC+S+G G
Sbjct: 213 SKLFHMNILNCKNITLTDIGIDAPPESLNTDGIHIGRSNGVNLIGAKIKTGDDCVSIGDG 272

Query: 182 SQNINITNVQCGPGHGISIGSLGQDPKELNVTKITVRHSIFKNTKNGVRIKSWSTPNPSI 241
           ++N+ + NV+CGPGHGISIGSLG+ P E  V  +TVR  + KNT NGVRIK+W    P I
Sbjct: 273 TENLIVENVECGPGHGISIGSLGRYPNEQPVKGVTVRKCLIKNTDNGVRIKTWPGSPPGI 332

Query: 242 VTKVSFIHLQMENVENPIIIDQNYCP--HCSS 263
            + + F  + M+NV  P++IDQ YCP  HC +
Sbjct: 333 ASNILFEDITMDNVSLPVLIDQEYCPYGHCKA 362

[Close]

BLAST of Lsi04G006260 vs. TAIR 10
Match: AT3G07850.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 221.9 bits (564), Expect = 6.4e-58
Identity = 108/278 (38.85%), Postives = 166/278 (59.71%), Query Frame = 0


Query: 2   AWEKGCGYDGSAVVLIPQGTFLVFPMKLEGPCHGSIKLQIDGNVLAPHDKLFIKGEY-WL 61
           AW++ C     + + +P+G ++V  ++ +GPC G + L+++GN  AP      K    W+
Sbjct: 92  AWKEACA--AGSTITVPKGEYMVESLEFKGPCKGPVTLELNGNFKAPATVKTTKPHAGWI 151

Query: 62  ALHQVNNLIVDGQGG-FDGQGSSAWGY-------RGHDRPITLKFTAINNATIRNIKSVD 121
               + +  ++G    FDGQGS AW         + +  PI ++FT + N+ I +I S +
Sbjct: 152 DFENIADFTLNGNKAIFDGQGSLAWKANDCAKTGKCNSLPINIRFTGLTNSKINSITSTN 211

Query: 122 SKFFHFAVHESENMTFDNVTLIAPKNSSNTDGIHISKSTNINIFNSVMSTGDDCISMGHG 181
           SK FH  +   +N+T  ++ + AP  S NTDGIHI +S  +N+  + + TGDDC+S+G G
Sbjct: 212 SKLFHMNILNCKNITLSDIGIDAPPESLNTDGIHIGRSNGVNLIGAKIKTGDDCVSIGDG 271

Query: 182 SQNINITNVQCGPGHGISIGSLGQDPKELNVTKITVRHSIFKNTKNGVRIKSWSTPNPSI 241
           ++N+ + NV+CGPGHGISIGSLG+ P E  V  +TVR  + KNT NGVRIK+W    P I
Sbjct: 272 TENLIVENVECGPGHGISIGSLGRYPNEQPVKGVTVRKCLIKNTDNGVRIKTWPGSPPGI 331

Query: 242 VTKVSFIHLQMENVENPIIIDQNYCP--HCSSNHQHQL 269
            + + F  + M+NV  P++IDQ YCP  HC +    Q+
Sbjct: 332 ASNILFEDITMDNVSLPVLIDQEYCPYGHCKAGVPSQV 367

The following BLAST results are available for this feature:

BLAST of Lsi04G006260 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q6H9K0	3.4e-64	44.85	Exopolygalacturonase (Fragment) OS=Platanus acerifolia OX=140101 GN=plaa2 PE=1 S...	[more]
Q05967	9.3e-62	42.86	Polygalacturonase OS=Nicotiana tabacum OX=4097 GN=PG1 PE=2 SV=1	[more]
P24548	2.3e-60	43.23	Exopolygalacturonase (Fragment) OS=Oenothera organensis OX=3945 PE=2 SV=1	[more]
Q40312	2.5e-59	41.84	Polygalacturonase OS=Medicago sativa OX=3879 PE=2 SV=1	[more]
P35338	1.1e-57	41.39	Exopolygalacturonase OS=Zea mays OX=4577 GN=PG9 PE=2 SV=1	[more]

BLAST of Lsi04G006260 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L7H9	2.1e-88	60.08	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G063610 PE=3 SV=1	[more]
A0A5D3BB58	2.8e-85	58.89	Exopolygalacturonase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffo...	[more]
A0A1S4DX91	4.7e-85	58.58	exopolygalacturonase-like OS=Cucumis melo OX=3656 GN=LOC103491147 PE=3 SV=1	[more]
A0A5D3B9J1	6.2e-85	58.21	Exopolygalacturonase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffo...	[more]
A0A6J1F153	1.7e-82	55.73	exopolygalacturonase-like OS=Cucurbita moschata OX=3662 GN=LOC111438612 PE=3 SV=...	[more]

BLAST of Lsi04G006260 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038904214.1	8.5e-89	60.22	exopolygalacturonase-like [Benincasa hispida]	[more]
XP_031738767.1	4.2e-88	60.08	exopolygalacturonase [Cucumis sativus]	[more]
XP_004149596.1	5.5e-88	59.33	exopolygalacturonase [Cucumis sativus]	[more]
XP_038904218.1	2.6e-85	58.71	exopolygalacturonase-like [Benincasa hispida]	[more]
KAA0065794.1	5.7e-85	58.89	exopolygalacturonase-like [Cucumis melo var. makuwa] >TYJ96533.1 exopolygalactur...	[more]

BLAST of Lsi04G006260 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G17150.1	9.8e-59	43.54	Pectin lyase-like superfamily protein	[more]
AT1G65570.1	4.9e-58	42.96	Pectin lyase-like superfamily protein	[more]
AT1G05660.1	4.9e-58	42.21	Pectin lyase-like superfamily protein	[more]
AT3G14040.1	6.4e-58	39.71	Pectin lyase-like superfamily protein	[more]
AT3G07850.1	6.4e-58	38.85	Pectin lyase-like superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006626	Parallel beta-helix repeat	SMART	SM00710	pbh1	coord: 203..224 e-value: 37.0 score: 13.2 coord: 173..193 e-value: 2400.0 score: 4.5 coord: 150..171 e-value: 2600.0 score: 4.3 coord: 123..149 e-value: 240.0 score: 10.5
IPR012334	Pectin lyase fold	GENE3D	2.160.20.10		coord: 1..268 e-value: 2.0E-76 score: 259.6
IPR000743	Glycoside hydrolase, family 28	PFAM	PF00295	Glyco_hydro_28	coord: 7..265 e-value: 1.9E-72 score: 244.0
IPR000743	Glycoside hydrolase, family 28	PROSITE	PS00502	POLYGALACTURONASE	coord: 180..193
None	No IPR available	PANTHER	PTHR31375:SF124	PECTIN LYASE-LIKE SUPERFAMILY PROTEIN	coord: 2..261
None	No IPR available	PANTHER	PTHR31375	FAMILY NOT NAMED	coord: 2..261
IPR011050	Pectin lyase fold/virulence factor	SUPERFAMILY	51126	Pectin lyase-like	coord: 2..261

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi04G006260.1	Lsi04G006260.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0005975	carbohydrate metabolic process
molecular_function	GO:0004650	polygalacturonase activity