Lsi04G006180 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi04G006180
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Descriptioncytochrome P450 71B37-like
Locationchr04: 5667483 .. 5669790 (+)
RNA-Seq ExpressionLsi04G006180
SyntenyLsi04G006180
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAAATTTGGAAGTCACAATAACAAGAAGGTTATTGTTTCTCCTCCAACCCCACCAAAGCTTCCTTTTTTAGGTCATTTACACCTCATTACCTCTCTCTCCCATCGCTCTTTTTCTAACCTTTCAAAAAAATATGGCCCCATCATCCTCCTCAAACTCGGCTCCATCCCGACTGTCGTAATCTCCTCTGCCACCGCCGCAAAAGAGGTGTTAAAAGTACACGACCTCGCTTCGTGCAGCCGACCTCAATCCACCACAGGTGCAAGATTTTCATACAACTATCTAGACATTGGGTTAGCGCCGTATGGAGATCATTGGAGAGAAGTTCGAAAGATTTGTGTGCTTGAGATGTTGAGTGCTAGAAGAGTGCAATCATTTCAAATGATAAGAGAAGAAGAGATTGGTGTGTTGTTAAATACCATTTCTCAATCTTCATCTTCTAACACTCCAATTAATCTTAGTGAGAAATTGTACTCTCTCACTGCAAATATAACAGCACGGATTGCTTTTGGGAAGTGCTTTAGAGGAAGTGAGTTAGATAATGAGAATTTTCAAATGATTATTCGAAGAGCAATGGCAATAATAGGAAGCTTTTCAGCGAATGACTTCTTTCCTAGAGTGGGTTGGATTATTGATTGGATTAGTGGTGTTCATACAAGATTGGAGAAGAGTTTTGCTGAGTTGGATGCTTTTTTTCAACATGTAGTGGATGATCGTATCAAGTTTAGGGAGATTTCTTCTCATAATGATTCTGATGATCAAGAAAACATTGTTGATGTTTTGTTGAAAATGGAGAAAGATGGCTCTGAATTTGATGTAGTGAAACTCCCTAGAGATTGCATCAAAGCACTTATCATGGTAAGCTTTCAACTTAATTGAACAAATAATATCTTCTTTAAGTTTATATCACATGATATTATCCAACTCAAAAAACCTTCATTTACTTTGTAATATATACAATGAAGATATATATATGGATTAGGGAAAATATATTTTTGGTCCCTAAATCTTGAGTCTAGTTTTCATTTGGTTTCTAAGTTTAAAAATGATACACTTTGGGGTTCTTAAATTTTGAATTTGATTTCAATTTAGTTCTTAAGTTTTAAAATGTTATAATTTTACCTTTAAGATTTTAGATTTACTTTAATTTTGGTTCCCATGTTTCAAGATTTTACACTTTTAACCTCGCTAATTTTTTTACCGAATACTCCCTTTCGTTTTTAGTATTAATGTCTATTAATTTATTTAAAAGAACTAAAAATTATAATTAATAAAATTTCACTATTTTTTTTATCACTATTAAAATTAAATTTAAAATTTCACTTCATAATTGTTTTAAATAAATTAATAGACATTAACACTAATGACAATTGAAAGTGAGTATCTAGTAAAAATCAAAGTTTAAAAGTGTAAATTTTGAAACATGGGAACCAAATTAACACGAAACTCAAATCGGAAGGGTAAAATAACATTTAGAAACCTAAGGATTAAATTGAAACCAAACTCAAAACTTAAAAACTAAAAGTGTAACATTTTGAAACCTAAGGTCTAAATATAAACTATACCCAAAACCTAGAAGTTCTTTTTCCCTATTTTATTGTTATATATTTTTCTCCACTTGTATTTGTACAATTTTTTTTTTTTTAAATTTGTCTTTTATTCTTCTTTTGTGTGTGCGTGATTTTACGATATATTTCTAGCTGGGGTGGAAACAGGAGCAAACACACTTGTTTGGGCAATGACAGAGTTGGTAAAAAATCCAAAGGTGATGAAAAAGCTACAAGGTGAGATTAGAAGTTGCGTAAAAGAAAATGAAGCAATAAAAGAGAGTGATCTCGAAAAGCTTAAATATCTAAAAGCAGTAGTGAAAGAAGTTCTAAGGTTGCATGCACCCGTCCCACTTCTCCTTCCAAGAGAAGCCATGTCCCATTTTAAGTTAAATGGTTATGATATTCATCCAAAAACTCGTGTTTATGTCAACGTGTGGGCAATCGGACGAGACCCAAAATTATGGACTAATCCAGAAGCGTTCTTGCCAGAGAGATTTATAGGAAGTAATATTGATTACAAAGGGCAGAATTTTGAGTTATTACCGTTTGGATCTGGTCGAAGAATTTGTCCTGGAATGAACATGGCAACTATTATAGTGGAGCTAGCACTAACTAATATATTGTTGTGTTTTGATTGGAAATTACCAAATGAAATGAAAGAAGAAGATGTGGATATGGAAGAGGAATTTGGTATTGCAGTTGCCAAGAAATCACCTCTTCAACTTGTTCCAGTCCATTACTTCAACTCCAAAGAATAG

mRNA sequence

ATGAAATTTGGAAGTCACAATAACAAGAAGGTTATTGTTTCTCCTCCAACCCCACCAAAGCTTCCTTTTTTAGGTCATTTACACCTCATTACCTCTCTCTCCCATCGCTCTTTTTCTAACCTTTCAAAAAAATATGGCCCCATCATCCTCCTCAAACTCGGCTCCATCCCGACTGTCGTAATCTCCTCTGCCACCGCCGCAAAAGAGGTGTTAAAAGTACACGACCTCGCTTCGTGCAGCCGACCTCAATCCACCACAGGTGCAAGATTTTCATACAACTATCTAGACATTGGGTTAGCGCCGTATGGAGATCATTGGAGAGAAGTTCGAAAGATTTGTGTGCTTGAGATGTTGAGTGCTAGAAGAGTGCAATCATTTCAAATGATAAGAGAAGAAGAGATTGGTGTGTTGTTAAATACCATTTCTCAATCTTCATCTTCTAACACTCCAATTAATCTTAGTGAGAAATTGTACTCTCTCACTGCAAATATAACAGCACGGATTGCTTTTGGGAAGTGCTTTAGAGGAAGTGAGTTAGATAATGAGAATTTTCAAATGATTATTCGAAGAGCAATGGCAATAATAGGAAGCTTTTCAGCGAATGACTTCTTTCCTAGAGTGGGTTGGATTATTGATTGGATTAGTGGTGTTCATACAAGATTGGAGAAGAGTTTTGCTGAGTTGGATGCTTTTTTTCAACATGTAGTGGATGATCGTATCAAGTTTAGGGAGATTTCTTCTCATAATGATTCTGATGATCAAGAAAACATTGTTGATGTTTTGTTGAAAATGGAGAAAGATGGCTCTGAATTTGATGTAGTGAAACTCCCTAGAGATTGCATCAAAGCACTTATCATGGTGATGAAAAAGCTACAAGGTGAGATTAGAAGTTGCGTAAAAGAAAATGAAGCAATAAAAGAGAGTGATCTCGAAAAGCTTAAATATCTAAAAGCAGTAGTGAAAGAAGTTCTAAGGTTGCATGCACCCGTCCCACTTCTCCTTCCAAGAGAAGCCATGTCCCATTTTAAGTTAAATGGTTATGATATTCATCCAAAAACTCGTGTTTATGTCAACGTGTGGGCAATCGGACGAGACCCAAAATTATGGACTAATCCAGAAGCGTTCTTGCCAGAGAGATTTATAGGAAGTAATATTGATTACAAAGGGCAGAATTTTGAGTTATTACCGTTTGGATCTGGTCGAAGAATTTGTCCTGGAATGAACATGGCAACTATTATAGTGGAGCTAGCACTAACTAATATATTGTTGTGTTTTGATTGGAAATTACCAAATGAAATGAAAGAAGAAGATGTGGATATGGAAGAGGAATTTGGTATTGCAGTTGCCAAGAAATCACCTCTTCAACTTGTTCCAGTCCATTACTTCAACTCCAAAGAATAG

Coding sequence (CDS)

ATGAAATTTGGAAGTCACAATAACAAGAAGGTTATTGTTTCTCCTCCAACCCCACCAAAGCTTCCTTTTTTAGGTCATTTACACCTCATTACCTCTCTCTCCCATCGCTCTTTTTCTAACCTTTCAAAAAAATATGGCCCCATCATCCTCCTCAAACTCGGCTCCATCCCGACTGTCGTAATCTCCTCTGCCACCGCCGCAAAAGAGGTGTTAAAAGTACACGACCTCGCTTCGTGCAGCCGACCTCAATCCACCACAGGTGCAAGATTTTCATACAACTATCTAGACATTGGGTTAGCGCCGTATGGAGATCATTGGAGAGAAGTTCGAAAGATTTGTGTGCTTGAGATGTTGAGTGCTAGAAGAGTGCAATCATTTCAAATGATAAGAGAAGAAGAGATTGGTGTGTTGTTAAATACCATTTCTCAATCTTCATCTTCTAACACTCCAATTAATCTTAGTGAGAAATTGTACTCTCTCACTGCAAATATAACAGCACGGATTGCTTTTGGGAAGTGCTTTAGAGGAAGTGAGTTAGATAATGAGAATTTTCAAATGATTATTCGAAGAGCAATGGCAATAATAGGAAGCTTTTCAGCGAATGACTTCTTTCCTAGAGTGGGTTGGATTATTGATTGGATTAGTGGTGTTCATACAAGATTGGAGAAGAGTTTTGCTGAGTTGGATGCTTTTTTTCAACATGTAGTGGATGATCGTATCAAGTTTAGGGAGATTTCTTCTCATAATGATTCTGATGATCAAGAAAACATTGTTGATGTTTTGTTGAAAATGGAGAAAGATGGCTCTGAATTTGATGTAGTGAAACTCCCTAGAGATTGCATCAAAGCACTTATCATGGTGATGAAAAAGCTACAAGGTGAGATTAGAAGTTGCGTAAAAGAAAATGAAGCAATAAAAGAGAGTGATCTCGAAAAGCTTAAATATCTAAAAGCAGTAGTGAAAGAAGTTCTAAGGTTGCATGCACCCGTCCCACTTCTCCTTCCAAGAGAAGCCATGTCCCATTTTAAGTTAAATGGTTATGATATTCATCCAAAAACTCGTGTTTATGTCAACGTGTGGGCAATCGGACGAGACCCAAAATTATGGACTAATCCAGAAGCGTTCTTGCCAGAGAGATTTATAGGAAGTAATATTGATTACAAAGGGCAGAATTTTGAGTTATTACCGTTTGGATCTGGTCGAAGAATTTGTCCTGGAATGAACATGGCAACTATTATAGTGGAGCTAGCACTAACTAATATATTGTTGTGTTTTGATTGGAAATTACCAAATGAAATGAAAGAAGAAGATGTGGATATGGAAGAGGAATTTGGTATTGCAGTTGCCAAGAAATCACCTCTTCAACTTGTTCCAGTCCATTACTTCAACTCCAAAGAATAG

Protein sequence

MKFGSHNNKKVIVSPPTPPKLPFLGHLHLITSLSHRSFSNLSKKYGPIILLKLGSIPTVVISSATAAKEVLKVHDLASCSRPQSTTGARFSYNYLDIGLAPYGDHWREVRKICVLEMLSARRVQSFQMIREEEIGVLLNTISQSSSSNTPINLSEKLYSLTANITARIAFGKCFRGSELDNENFQMIIRRAMAIIGSFSANDFFPRVGWIIDWISGVHTRLEKSFAELDAFFQHVVDDRIKFREISSHNDSDDQENIVDVLLKMEKDGSEFDVVKLPRDCIKALIMVMKKLQGEIRSCVKENEAIKESDLEKLKYLKAVVKEVLRLHAPVPLLLPREAMSHFKLNGYDIHPKTRVYVNVWAIGRDPKLWTNPEAFLPERFIGSNIDYKGQNFELLPFGSGRRICPGMNMATIIVELALTNILLCFDWKLPNEMKEEDVDMEEEFGIAVAKKSPLQLVPVHYFNSKE
Homology
BLAST of Lsi04G006180 vs. ExPASy Swiss-Prot
Match: Q9LIP3 (Cytochrome P450 71B37 OS=Arabidopsis thaliana OX=3702 GN=CYP71B37 PE=3 SV=2)

HSP 1 Score: 466.8 bits (1200), Expect = 2.8e-130
Identity = 233/475 (49.05%), Postives = 312/475 (65.68%), Query Frame = 0

Query: 15  PPTPPKLPFLGHLHLITSLSHRSFSNLSKKYGPIILLKLGSIPTVVISSATAAKEVLKVH 74
           PP+PP  P +G+LH +  L H+S  +LSKKYGP++LLK GSIPTVV+SS+  AK+ LK+H
Sbjct: 32  PPSPPGFPIIGNLHQLGELPHQSLWSLSKKYGPVMLLKFGSIPTVVVSSSETAKQALKIH 91

Query: 75  DLASCSRPQSTTGARFSYNYLDIGLAPYGDHWREVRKICVLEMLSARRVQSFQMIREEEI 134
           DL  CSRP        SYNYLDI  +P+ D+W+E+R++CV E+ S ++V   Q IREEE+
Sbjct: 92  DLNCCSRPSLAGPRALSYNYLDIVFSPFNDYWKELRRMCVQELFSPKQVHLIQPIREEEV 151

Query: 135 GVLLNTISQSSSSNTPINLSEKLYSLTANITARIAFGKCFRGSELDNENFQMIIRRAMAI 194
             L+N+ S+S++  TP+NLSEKL SLT  +  + AFG  F+G+ L+++NF  +I  A   
Sbjct: 152 KKLMNSFSESAAQKTPVNLSEKLASLTVGVICKAAFGVSFQGTVLNSDNFDKLIHDAFLF 211

Query: 195 IGSFSANDFFPRVGWIIDWISGVHTRLEKSFAELDAFFQHVVDDRIKFREISSHNDSDDQ 254
           +GSFSA+D+FP VGWIIDW++G+  + E+S   LDAF++ + D       +    + +  
Sbjct: 212 LGSFSASDYFPNVGWIIDWLTGLQGQRERSVRGLDAFYEQMFD-------LHKQGNKEGV 271

Query: 255 ENIVDVLLKMEKDGSEFDVVKLPRDCIKALIM--------------------------VM 314
           E+ VD+LLK+EK+ +     KL R+ IKA++M                          VM
Sbjct: 272 EDFVDLLLKLEKEETVLGYGKLTRNHIKAVLMNVLLGGIGTSAITMTWAMTELMRNPRVM 331

Query: 315 KKLQGEIRSCVKENEAIKESDLEKLKYLKAVVKEVLRLHAPVPLLLPREAMSHFKLNGYD 374
           KK+Q EIR+ +     I   D+++L YLK V+ E  RLH P PLL+PRE MS F++NGY 
Sbjct: 332 KKVQSEIRNQIGGKSMICLDDIDQLHYLKMVINETWRLHPPAPLLVPREVMSEFEINGYT 391

Query: 375 IHPKTRVYVNVWAIGRDPKLWTNPEAFLPERFIGSNIDYKGQNFELLPFGSGRRICPGMN 434
           I  KTR+YVNVW IGRDP  W +PE FLPERF+ SNID KGQNFELLPFGSGRR+CP M 
Sbjct: 392 IPAKTRLYVNVWGIGRDPDTWKDPEEFLPERFVNSNIDAKGQNFELLPFGSGRRMCPAMY 451

Query: 435 MATIIVELALTNILLCFDWKLPNEMKEEDVDMEEEFGIAVAKKSPLQLVPVHYFN 464
           M T +VE  L N+L  FDWKLP  M  ED+DMEE  G+  +KK+ L LVP  Y N
Sbjct: 452 MGTTMVEFGLANLLYHFDWKLPEGMVVEDIDMEESPGLNASKKNELVLVPRKYLN 499

BLAST of Lsi04G006180 vs. ExPASy Swiss-Prot
Match: O64718 (Cytochrome P450 71B9 OS=Arabidopsis thaliana OX=3702 GN=CYP71B9 PE=2 SV=3)

HSP 1 Score: 461.8 bits (1187), Expect = 8.9e-129
Identity = 232/473 (49.05%), Postives = 310/473 (65.54%), Query Frame = 0

Query: 15  PPTPPKLPFLGHLHLITSLSHRSFSNLSKKYGPIILLKLGSIPTVVISSATAAKEVLKVH 74
           PP+PP  P +G+LH +  L H+S  +LSK YGP++LLKLGS+PTVV+SS+  AK+VLK++
Sbjct: 32  PPSPPGFPIIGNLHQLGELPHQSLWSLSKTYGPVMLLKLGSVPTVVVSSSETAKQVLKIN 91

Query: 75  DLASCSRPQSTTGARFSYNYLDIGLAPYGDHWREVRKICVLEMLSARRVQSFQMIREEEI 134
           DL  CSRP        SYNYLDI  +P+ D+W+E+R+ICV E+ SA+RV S Q I+EEE+
Sbjct: 92  DLHCCSRPSLAGAKELSYNYLDIAFSPFDDYWKELRRICVQELFSAKRVHSIQPIKEEEV 151

Query: 135 GVLLNTISQSSSSNTPINLSEKLYSLTANITARIAFGKCFRGSELDNENFQMIIRRAMAI 194
             L+ + ++S+S  +P+NLSEK   LT ++  + AF   F  S L+N+ F  +I  A   
Sbjct: 152 RKLIVSATESASQKSPVNLSEKFLDLTVSVICKAAFSLDFHTSVLNNDGFDKLIHDAFLF 211

Query: 195 IGSFSANDFFPRVGWIIDWISGVHTRLEKSFAELDAFFQHVVDDRIKFREISSHNDSDDQ 254
           +GSFSA++FFP  GWIIDW++G+  R EKS  +LD F+Q + D       +    +    
Sbjct: 212 LGSFSASNFFPNGGWIIDWLTGLQRRREKSVKDLDVFYQQMFD-------LHKQENKQGV 271

Query: 255 ENIVDVLLKMEKDGSEFDVVKLPRDCIKALIM--------------------------VM 314
           E+ VD+LLK+EK+ +     KL R+ +KA++M                          VM
Sbjct: 272 EDFVDLLLKLEKEETVLGYGKLTRNHVKAILMNVLLGAINTSAMTMTWAMAELIRNPRVM 331

Query: 315 KKLQGEIRSCVKENEAIKESDLEKLKYLKAVVKEVLRLHAPVPLLLPREAMSHFKLNGYD 374
           KK+Q EIR+ +     I   D++ L YLK V+KE  RLH PVPLLLPRE MS F++NGY 
Sbjct: 332 KKVQSEIRNQMINKSVITLDDIDHLPYLKMVIKETWRLHPPVPLLLPREVMSEFEINGYK 391

Query: 375 IHPKTRVYVNVWAIGRDPKLWTNPEAFLPERFIGSNIDYKGQNFELLPFGSGRRICPGMN 434
           I PKT +YVNVWAIGRDP  W + + F PERF+ +NID KGQNFELLPFGSGRRICPGM 
Sbjct: 392 IQPKTLLYVNVWAIGRDPDSWKDADMFYPERFMDNNIDAKGQNFELLPFGSGRRICPGMY 451

Query: 435 MATIIVELALTNILLCFDWKLPNEMKEEDVDMEEEFGIAVAKKSPLQLVPVHY 462
           M T +VE  L N+L  FDW++P+ M  ED+DMEE  G+AV KK+ L LVPV Y
Sbjct: 452 MGTTMVEFGLANMLYQFDWEVPDGMVVEDIDMEESPGLAVGKKNELLLVPVKY 497

BLAST of Lsi04G006180 vs. ExPASy Swiss-Prot
Match: Q9LIP4 (Cytochrome P450 71B36 OS=Arabidopsis thaliana OX=3702 GN=CYP71B36 PE=3 SV=1)

HSP 1 Score: 458.0 bits (1177), Expect = 1.3e-127
Identity = 226/475 (47.58%), Postives = 314/475 (66.11%), Query Frame = 0

Query: 15  PPTPPKLPFLGHLHLITSLSHRSFSNLSKKYGPIILLKLGSIPTVVISSATAAKEVLKVH 74
           PP+PP  P +G+LH +  L H+S   LSKKYG ++LLK GSIPTVV+SS+  AK+VLK+H
Sbjct: 32  PPSPPGFPIIGNLHQLGELPHQSLWRLSKKYGHVMLLKFGSIPTVVVSSSETAKQVLKIH 91

Query: 75  DLASCSRPQSTTGARFSYNYLDIGLAPYGDHWREVRKICVLEMLSARRVQSFQMIREEEI 134
           DL  CSRP        SYNYLDI  +P+ D+W+E+R+ICV E+ S +RVQSFQ I+E+E+
Sbjct: 92  DLHCCSRPSLAGPRALSYNYLDIAFSPFDDYWKELRRICVQELFSVKRVQSFQPIKEDEV 151

Query: 135 GVLLNTISQSSSSNTPINLSEKLYSLTANITARIAFGKCFRGSELDNENFQMIIRRAMAI 194
             L++++S+S+S  TP+NLSEK  SLT  +T +  FG  F+G+ L+++ F+ +I      
Sbjct: 152 KKLIDSVSESASQGTPVNLSEKFTSLTVRVTCKATFGVNFQGTVLNSDRFEKLIHDTYLF 211

Query: 195 IGSFSANDFFPRVGWIIDWISGVHTRLEKSFAELDAFFQHVVDDRIKFREISSHNDSDDQ 254
           +GSFSA+D+FP  GWIIDW++G+H + E+S   LDAF++ + D       +    + +  
Sbjct: 212 LGSFSASDYFPNGGWIIDWLTGLHGQRERSVRALDAFYEQMFD-------LHKQGNKEGV 271

Query: 255 ENIVDVLLKMEKDGSEFDVVKLPRDCIKALIM--------------------------VM 314
           E+ VD+LL++EK+ +     KL R+ IKA++M                          VM
Sbjct: 272 EDFVDLLLRLEKEETVIGYGKLTRNHIKAILMNVLIGGIGTSAITMTWAMTELMRNPRVM 331

Query: 315 KKLQGEIRSCVKENEAIKESDLEKLKYLKAVVKEVLRLHAPVPLLLPREAMSHFKLNGYD 374
           KK+Q EIR+ + +   I   D+++L YLK V+ E  RLH P P L+PR+ MS F+LN Y 
Sbjct: 332 KKVQSEIRNQIGKKSMITLDDIDQLHYLKMVINETWRLHPPSPFLIPRQVMSEFELNDYV 391

Query: 375 IHPKTRVYVNVWAIGRDPKLWTNPEAFLPERFIGSNIDYKGQNFELLPFGSGRRICPGMN 434
           I  KTR+YVNVWAIGRDP  W +PE FLPERF+ S+ID KGQ+FELLPFGSGRR+CP M 
Sbjct: 392 IPVKTRLYVNVWAIGRDPDTWKDPEEFLPERFVNSSIDAKGQHFELLPFGSGRRMCPAMY 451

Query: 435 MATIIVELALTNILLCFDWKLPNEMKEEDVDMEEEFGIAVAKKSPLQLVPVHYFN 464
           M T +VE  L N+L  FDWK+P  M  ED+D+EE  G+  +KK+ L LVP+ Y +
Sbjct: 452 MGTTMVEFGLANMLYHFDWKIPVGMVAEDIDLEESPGLNASKKNELVLVPLKYLD 499

BLAST of Lsi04G006180 vs. ExPASy Swiss-Prot
Match: Q9LIP6 (Cytochrome P450 71B34 OS=Arabidopsis thaliana OX=3702 GN=CYP71B34 PE=2 SV=1)

HSP 1 Score: 456.4 bits (1173), Expect = 3.7e-127
Identity = 230/487 (47.23%), Postives = 317/487 (65.09%), Query Frame = 0

Query: 3   FGSHNNKKVIVSPPTPPKLPFLGHLHLITSLSHRSFSNLSKKYGPIILLKLGSIPTVVIS 62
           F   N +    +PP+PP  P +G+LH +  L H+S   LSKKYGP++LLKLG +PTV++S
Sbjct: 20  FNHKNRRNYQRTPPSPPGCPIIGNLHQLGELPHQSLWKLSKKYGPVMLLKLGRVPTVIVS 79

Query: 63  SATAAKEVLKVHDLASCSRPQSTTGARFSYNYLDIGLAPYGDHWREVRKICVLEMLSARR 122
           S+  AK+ LK+HDL  CSRP        SYNYLDI  +PY D+W+EVRK+ V E+ S+++
Sbjct: 80  SSETAKQALKIHDLHCCSRPGFAGARELSYNYLDIAFSPYDDYWKEVRKLAVQELFSSKQ 139

Query: 123 VQSFQMIREEEIGVLLNTISQSSSSNTPINLSEKLYSLTANITARIAFGKCFRGSELDNE 182
           V S Q I++EE+  L+++IS+S++  TPINL++ L +LT ++  R AF   F G+ L++E
Sbjct: 140 VHSIQPIKDEEVKKLIDSISESAAQKTPINLNKTLLALTVSVVCRTAFSVNFEGTVLNSE 199

Query: 183 NFQMIIRRAMAIIGSFSANDFFPRVGWIIDWISGVHTRLEKSFAELDAFFQHVVDDRIKF 242
            F  I+R A+ ++GSFSA+DF P VG IID ++G+  R E+S  +LDAF++ + D     
Sbjct: 200 RFNNIVREALEMLGSFSASDFIPYVGRIIDLLTGLQGRRERSMRDLDAFYEQMFD----- 259

Query: 243 REISSHNDSDDQENIVDVLLKMEKDGSEFDVVKLPRDCIKALIM---------------- 302
             +      +  E+ VD+LL++EK+ +     KL R+ IKA++M                
Sbjct: 260 --LHKQKKEEGSEDFVDLLLRLEKEEAVLGNDKLTRNHIKAILMDVLLAGMDTSAITMTW 319

Query: 303 ----------VMKKLQGEIRSCVKENEAIKESDLEKLKYLKAVVKEVLRLHAPVPLLLPR 362
                     VMKK+Q EIRS +K  E I   D +KL+YLK V+KE  RLH   PLL+PR
Sbjct: 320 AMAELAKNPRVMKKVQSEIRSQIKNKERISFDDTDKLEYLKMVIKETWRLHPTTPLLIPR 379

Query: 363 EAMSHFKLNGYDIHPKTRVYVNVWAIGRDPKLWTNPEAFLPERFIGSNIDYKGQNFELLP 422
           EAMS F++NGY I  KTR++VNVWAIGRDP  W +PE FLPERF  +NID KGQ+FELLP
Sbjct: 380 EAMSEFEINGYTIPVKTRLHVNVWAIGRDPDTWKDPEVFLPERFTDNNIDAKGQHFELLP 439

Query: 423 FGSGRRICPGMNMATIIVELALTNILLCFDWKLPNEMKEEDVDMEEEFGIAVAKKSPLQL 464
           FG GRR+CP + M T +VE  L N+L  FDWKLP  MK +D+DMEE  G+ V KK+ L L
Sbjct: 440 FGGGRRMCPAVYMGTTMVEFGLANLLYHFDWKLPEGMKVDDIDMEEAPGLTVNKKNELIL 499

BLAST of Lsi04G006180 vs. ExPASy Swiss-Prot
Match: Q9LTL0 (Cytochrome P450 71B26 OS=Arabidopsis thaliana OX=3702 GN=CYP71B26 PE=2 SV=1)

HSP 1 Score: 451.1 bits (1159), Expect = 1.6e-125
Identity = 226/474 (47.68%), Postives = 313/474 (66.03%), Query Frame = 0

Query: 16  PTPPKLPFLGHLHLITSLSHRSFSNLSKKYGPIILLKLGSIPTVVISSATAAKEVLKVHD 75
           P+PP  P +G+LH +  L H+S   LSKKYGP++LLKLG +PT+++SS+  AK+ L+ +D
Sbjct: 33  PSPPGFPIIGNLHQLGELQHQSLWKLSKKYGPVMLLKLGKVPTLILSSSETAKQALRDYD 92

Query: 76  LASCSRPQSTTGARFSYNYLDIGLAPYGDHWREVRKICVLEMLSARRVQSFQMIREEEIG 135
           L  CSRP    G   SYN LD+  +PY ++W+E+RK+C  E+ SA ++QS Q I++EE+ 
Sbjct: 93  LHCCSRPSLAGGRELSYNNLDMSSSPYNEYWKELRKLCSQELFSANKIQSIQPIKDEEVK 152

Query: 136 VLLNTISQSSSSNTPINLSEKLYSLTANITARIAFGKCFRGSELDNENFQMIIRRAMAII 195
            ++++I++SSS   P+NLS+   +LT ++  + AFG  F GS L+++ F  ++R    ++
Sbjct: 153 KVIDSIAESSSLKNPVNLSKTFLALTTSVVCKAAFGVSFEGSVLNSDRFNKLVRDTFEML 212

Query: 196 GSFSANDFFPRVGWIIDWISGVHTRLEKSFAELDAFFQHVVDDRIKFREISSHNDSDDQE 255
           GSFSA+DF P VGWIID  +G+    +KSF +LDAF++ + D   + +E+ S       E
Sbjct: 213 GSFSASDFIPYVGWIIDKFNGLQGWRKKSFRDLDAFYEQIFDLHKEEKEVGS-------E 272

Query: 256 NIVDVLLKMEKDGSEFDVVKLPRDCIKALIM--------------------------VMK 315
           ++VDVLL++EK+       KL R+ IKA++M                          VMK
Sbjct: 273 DLVDVLLRLEKEEIVVGNGKLTRNHIKAILMNILLGGIDTSAITMTWAMAELAKNPRVMK 332

Query: 316 KLQGEIRSCVKENEAIKESDLEKLKYLKAVVKEVLRLHAPVPLLLPREAMSHFKLNGYDI 375
           K+Q EIR+ +K  E I   D +KL+YLK V+KE  RLH P PLLLPR+ ++ F++NGY I
Sbjct: 333 KVQAEIRNQIKNKERISFDDTDKLEYLKMVIKETWRLHPPTPLLLPRDVITEFEINGYTI 392

Query: 376 HPKTRVYVNVWAIGRDPKLWTNPEAFLPERFIGSNIDYKGQNFELLPFGSGRRICPGMNM 435
             KTR++VNVWAIGRDP  W +PE FLPERF  SNID KGQNFELL FGSGRRICPG+ M
Sbjct: 393 PAKTRLHVNVWAIGRDPDTWKDPEMFLPERFNDSNIDAKGQNFELLSFGSGRRICPGLYM 452

Query: 436 ATIIVELALTNILLCFDWKLPNEMKEEDVDMEEEFGIAVAKKSPLQLVPVHYFN 464
            T +VE  L N+L  FDWKLP  M  ED+DMEE  G+ V+KKS L LVPV Y +
Sbjct: 453 GTTMVEFGLANMLYHFDWKLPEGMVVEDIDMEEAPGLTVSKKSELVLVPVKYLD 499

BLAST of Lsi04G006180 vs. ExPASy TrEMBL
Match: A0A5D3CWC3 (Cytochrome P450 71B37-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold35G001670 PE=3 SV=1)

HSP 1 Score: 729.6 bits (1882), Expect = 8.5e-207
Identity = 364/488 (74.59%), Postives = 406/488 (83.20%), Query Frame = 0

Query: 8   NKKVIVSPPTPPKLPFLGHLHLITSLSHRSFSNLSKKYGPIILLKLGSIPTVVISSATAA 67
           N  ++  PPTPPKLPFLGHLHLITSLSHRSF +LS++YGP++LL+LGSIPTVV+SSA AA
Sbjct: 4   NNVIVHLPPTPPKLPFLGHLHLITSLSHRSFRHLSEEYGPVMLLQLGSIPTVVVSSAAAA 63

Query: 68  KEVLKVHDLASCSRPQSTTGARFSYNYLDIGLAPYGDHWREVRKICVLEMLSARRVQSFQ 127
           KEVLKVHDLASCSRP+S   ARFSYNYLDIGLAPYG+HWREVRKICVLE+ SARRVQSFQ
Sbjct: 64  KEVLKVHDLASCSRPRSVANARFSYNYLDIGLAPYGEHWREVRKICVLELFSARRVQSFQ 123

Query: 128 MIREEEIGVLLNTISQSS-SSNTPINLSEKLYSLTANITARIAFGKCFRGSELDNENFQM 187
            IREEEIGV+LN+ISQSS  SN PI+LSEK YSLTANI  RIAFGK FRG ELDNENFQ 
Sbjct: 124 KIREEEIGVMLNSISQSSLLSNDPIDLSEKFYSLTANIITRIAFGKKFRGGELDNENFQK 183

Query: 188 IIRRAMAIIGSFSANDFFPRVGWIIDWISGVHTRLEKSFAELDAFFQHVVDDRIKFRE-- 247
           +IRRAMA +GS SA D+FP+VGWIIDW++GVH RLE SF ELD FFQH+VDDRIKFRE  
Sbjct: 184 VIRRAMAAVGSSSAADYFPKVGWIIDWVNGVHRRLEMSFNELDIFFQHIVDDRIKFRESP 243

Query: 248 -ISSHNDSDDQENIVDVLLKMEKDGSEFDVVKLPRDCIKALIM----------------- 307
             S  ND D+QENIVDVLLKMEK+ S+F  +KL RDCIKALIM                 
Sbjct: 244 RSSQDNDDDEQENIVDVLLKMEKNSSQFGEMKLTRDCIKALIMDIFLAGVETGATTLVWA 303

Query: 308 ---------VMKKLQGEIRSCVKENEAIKESDLEKLKYLKAVVKEVLRLHAPVPLLLPRE 367
                    VMKKLQ EIR+CVKE+  +KESDL+ L+YLKAVVKEVLRLH P PLLLPRE
Sbjct: 304 MTELIKNPKVMKKLQNEIRNCVKESTIVKESDLQNLEYLKAVVKEVLRLHTPAPLLLPRE 363

Query: 368 AMSHFKLNGYDIHPKTRVYVNVWAIGRDPKLWTNPEAFLPERFIGSNIDYKGQNFELLPF 427
           AMSHFKLNGYDI PKT ++VNVWAIGRD ++WTNPE F+PERFIGSNIDYKGQNFE LPF
Sbjct: 364 AMSHFKLNGYDILPKTHIHVNVWAIGRDSEIWTNPEEFIPERFIGSNIDYKGQNFEFLPF 423

Query: 428 GSGRRICPGMNMATIIVELALTNILLCFDWKLPNEMKEEDVDMEEEFGIAVAKKSPLQLV 466
           GSGRRICPGMNMA+  VELAL N+LLCFDWKLPN +KEED+DMEEE G+  +KKSPLQLV
Sbjct: 424 GSGRRICPGMNMASFTVELALANMLLCFDWKLPNGVKEEDIDMEEELGLTASKKSPLQLV 483

BLAST of Lsi04G006180 vs. ExPASy TrEMBL
Match: A0A1S4DW34 (cytochrome P450 71B37-like OS=Cucumis melo OX=3656 GN=LOC103488948 PE=3 SV=1)

HSP 1 Score: 729.6 bits (1882), Expect = 8.5e-207
Identity = 364/488 (74.59%), Postives = 406/488 (83.20%), Query Frame = 0

Query: 8   NKKVIVSPPTPPKLPFLGHLHLITSLSHRSFSNLSKKYGPIILLKLGSIPTVVISSATAA 67
           N  ++  PPTPPKLPFLGHLHLITSLSHRSF +LS++YGP++LL+LGSIPTVV+SSA AA
Sbjct: 57  NNVIVHLPPTPPKLPFLGHLHLITSLSHRSFRHLSEEYGPVMLLQLGSIPTVVVSSAAAA 116

Query: 68  KEVLKVHDLASCSRPQSTTGARFSYNYLDIGLAPYGDHWREVRKICVLEMLSARRVQSFQ 127
           KEVLKVHDLASCSRP+S   ARFSYNYLDIGLAPYG+HWREVRKICVLE+ SARRVQSFQ
Sbjct: 117 KEVLKVHDLASCSRPRSVANARFSYNYLDIGLAPYGEHWREVRKICVLELFSARRVQSFQ 176

Query: 128 MIREEEIGVLLNTISQSS-SSNTPINLSEKLYSLTANITARIAFGKCFRGSELDNENFQM 187
            IREEEIGV+LN+ISQSS  SN PI+LSEK YSLTANI  RIAFGK FRG ELDNENFQ 
Sbjct: 177 KIREEEIGVMLNSISQSSLLSNDPIDLSEKFYSLTANIITRIAFGKKFRGGELDNENFQK 236

Query: 188 IIRRAMAIIGSFSANDFFPRVGWIIDWISGVHTRLEKSFAELDAFFQHVVDDRIKFRE-- 247
           +IRRAMA +GS SA D+FP+VGWIIDW++GVH RLE SF ELD FFQH+VDDRIKFRE  
Sbjct: 237 VIRRAMAAVGSSSAADYFPKVGWIIDWVNGVHRRLEMSFNELDIFFQHIVDDRIKFRESP 296

Query: 248 -ISSHNDSDDQENIVDVLLKMEKDGSEFDVVKLPRDCIKALIM----------------- 307
             S  ND D+QENIVDVLLKMEK+ S+F  +KL RDCIKALIM                 
Sbjct: 297 RSSQDNDDDEQENIVDVLLKMEKNSSQFGEMKLTRDCIKALIMDIFLAGVETGATTLVWA 356

Query: 308 ---------VMKKLQGEIRSCVKENEAIKESDLEKLKYLKAVVKEVLRLHAPVPLLLPRE 367
                    VMKKLQ EIR+CVKE+  +KESDL+ L+YLKAVVKEVLRLH P PLLLPRE
Sbjct: 357 MTELIKNPKVMKKLQNEIRNCVKESTIVKESDLQNLEYLKAVVKEVLRLHTPAPLLLPRE 416

Query: 368 AMSHFKLNGYDIHPKTRVYVNVWAIGRDPKLWTNPEAFLPERFIGSNIDYKGQNFELLPF 427
           AMSHFKLNGYDI PKT ++VNVWAIGRD ++WTNPE F+PERFIGSNIDYKGQNFE LPF
Sbjct: 417 AMSHFKLNGYDILPKTHIHVNVWAIGRDSEIWTNPEEFIPERFIGSNIDYKGQNFEFLPF 476

Query: 428 GSGRRICPGMNMATIIVELALTNILLCFDWKLPNEMKEEDVDMEEEFGIAVAKKSPLQLV 466
           GSGRRICPGMNMA+  VELAL N+LLCFDWKLPN +KEED+DMEEE G+  +KKSPLQLV
Sbjct: 477 GSGRRICPGMNMASFTVELALANMLLCFDWKLPNGVKEEDIDMEEELGLTASKKSPLQLV 536

BLAST of Lsi04G006180 vs. ExPASy TrEMBL
Match: A0A6J1GWP7 (cytochrome P450 71B26-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111458149 PE=3 SV=1)

HSP 1 Score: 633.3 bits (1632), Expect = 8.3e-178
Identity = 315/483 (65.22%), Postives = 381/483 (78.88%), Query Frame = 0

Query: 6   HNNKKVIVSPPTPPKLPFLGHLHLITSLSHRSFSNLSKKYGPIILLKLGSIPTVVISSAT 65
           H+N K  + PP+P KLP LGHLHL+ SL HRS S LSKKYGP++LL+LGS+PT+VISSA 
Sbjct: 28  HSNPKNKLLPPSPLKLPLLGHLHLLGSLPHRSLSKLSKKYGPVMLLQLGSVPTIVISSAA 87

Query: 66  AAKEVLKVHDLASCSRPQSTTGARFSYNYLDIGLAPYGDHWREVRKICVLEMLSARRVQS 125
           AA+E+ K HDLASCSRP+ T   RFSYN+LD+ L+PYG+ WRE+RKIC+ E+ +ARRVQS
Sbjct: 88  AARELFKFHDLASCSRPRLTGSGRFSYNFLDLNLSPYGERWRELRKICMHELFTARRVQS 147

Query: 126 FQMIREEEIGVLLNTISQSSSSNTPINLSEKLYSLTANITARIAFGKCFRGSELDNENFQ 185
           F  IREEE+G L+ +ISQSSSS+  INLSEK YSLTANIT R+AFG  F G +LD+E+FQ
Sbjct: 148 FWEIREEEVGRLVKSISQSSSSSASINLSEKSYSLTANITTRVAFGSIFSGGKLDDEHFQ 207

Query: 186 MIIRRAMAIIGSFSANDFFPRVGWIIDWISGVHTRLEKSFAELDAFFQHVVDDRIKFREI 245
            +IRRA+A IGSFS  DF P  GWIID ++GVH RLEKSF E+DAFFQHVVDDRI F++ 
Sbjct: 208 HVIRRAVAAIGSFSMTDFIPTFGWIIDRLTGVHGRLEKSFGEMDAFFQHVVDDRINFKK- 267

Query: 246 SSHNDSDDQENIVDVLLKMEKDGSEFDVVKLPRDCIKALIM------------------- 305
                S ++ENIVDVLL+ME++ SE DV+++ +DC+KALIM                   
Sbjct: 268 ----SSKNEENIVDVLLRMERESSESDVLQVTQDCVKALIMDIFLAGVETGATTIVWIMT 327

Query: 306 -------VMKKLQGEIRSCVKENEAIKESDLEKLKYLKAVVKEVLRLHAPVPLLLPREAM 365
                  VMKKLQ EIRSC+KE +++KE DLEKL+YLK VVKE LRLH PVPLLLPR+ +
Sbjct: 328 ELVRNPRVMKKLQDEIRSCIKE-DSVKEMDLEKLEYLKMVVKEALRLHPPVPLLLPRKTI 387

Query: 366 SHFKLNGYDIHPKTRVYVNVWAIGRDPKLWTNPEAFLPERFIGSNIDYKGQNFELLPFGS 425
           S FKLNGY+I PKT +++N+WAIGRDP+ WT PE F PERFIGSNIDYKGQNFEL+PFGS
Sbjct: 388 SSFKLNGYNIDPKTHLHINMWAIGRDPQSWTKPEEFFPERFIGSNIDYKGQNFELIPFGS 447

Query: 426 GRRICPGMNMATIIVELALTNILLCFDWKLPNEMKEEDVDMEEEFGIAVAKKSPLQLVPV 463
           GRRICPGM+MAT+ VELAL N+LLCFDWKLP+ MKEEDVDMEE  G+A  KKSPL+L+PV
Sbjct: 448 GRRICPGMSMATLTVELALANLLLCFDWKLPDGMKEEDVDMEEADGLAAPKKSPLRLIPV 504

BLAST of Lsi04G006180 vs. ExPASy TrEMBL
Match: A0A6J1GW76 (cytochrome P450 71B26-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111458149 PE=3 SV=1)

HSP 1 Score: 633.3 bits (1632), Expect = 8.3e-178
Identity = 315/483 (65.22%), Postives = 381/483 (78.88%), Query Frame = 0

Query: 6   HNNKKVIVSPPTPPKLPFLGHLHLITSLSHRSFSNLSKKYGPIILLKLGSIPTVVISSAT 65
           H+N K  + PP+P KLP LGHLHL+ SL HRS S LSKKYGP++LL+LGS+PT+VISSA 
Sbjct: 34  HSNPKNKLLPPSPLKLPLLGHLHLLGSLPHRSLSKLSKKYGPVMLLQLGSVPTIVISSAA 93

Query: 66  AAKEVLKVHDLASCSRPQSTTGARFSYNYLDIGLAPYGDHWREVRKICVLEMLSARRVQS 125
           AA+E+ K HDLASCSRP+ T   RFSYN+LD+ L+PYG+ WRE+RKIC+ E+ +ARRVQS
Sbjct: 94  AARELFKFHDLASCSRPRLTGSGRFSYNFLDLNLSPYGERWRELRKICMHELFTARRVQS 153

Query: 126 FQMIREEEIGVLLNTISQSSSSNTPINLSEKLYSLTANITARIAFGKCFRGSELDNENFQ 185
           F  IREEE+G L+ +ISQSSSS+  INLSEK YSLTANIT R+AFG  F G +LD+E+FQ
Sbjct: 154 FWEIREEEVGRLVKSISQSSSSSASINLSEKSYSLTANITTRVAFGSIFSGGKLDDEHFQ 213

Query: 186 MIIRRAMAIIGSFSANDFFPRVGWIIDWISGVHTRLEKSFAELDAFFQHVVDDRIKFREI 245
            +IRRA+A IGSFS  DF P  GWIID ++GVH RLEKSF E+DAFFQHVVDDRI F++ 
Sbjct: 214 HVIRRAVAAIGSFSMTDFIPTFGWIIDRLTGVHGRLEKSFGEMDAFFQHVVDDRINFKK- 273

Query: 246 SSHNDSDDQENIVDVLLKMEKDGSEFDVVKLPRDCIKALIM------------------- 305
                S ++ENIVDVLL+ME++ SE DV+++ +DC+KALIM                   
Sbjct: 274 ----SSKNEENIVDVLLRMERESSESDVLQVTQDCVKALIMDIFLAGVETGATTIVWIMT 333

Query: 306 -------VMKKLQGEIRSCVKENEAIKESDLEKLKYLKAVVKEVLRLHAPVPLLLPREAM 365
                  VMKKLQ EIRSC+KE +++KE DLEKL+YLK VVKE LRLH PVPLLLPR+ +
Sbjct: 334 ELVRNPRVMKKLQDEIRSCIKE-DSVKEMDLEKLEYLKMVVKEALRLHPPVPLLLPRKTI 393

Query: 366 SHFKLNGYDIHPKTRVYVNVWAIGRDPKLWTNPEAFLPERFIGSNIDYKGQNFELLPFGS 425
           S FKLNGY+I PKT +++N+WAIGRDP+ WT PE F PERFIGSNIDYKGQNFEL+PFGS
Sbjct: 394 SSFKLNGYNIDPKTHLHINMWAIGRDPQSWTKPEEFFPERFIGSNIDYKGQNFELIPFGS 453

Query: 426 GRRICPGMNMATIIVELALTNILLCFDWKLPNEMKEEDVDMEEEFGIAVAKKSPLQLVPV 463
           GRRICPGM+MAT+ VELAL N+LLCFDWKLP+ MKEEDVDMEE  G+A  KKSPL+L+PV
Sbjct: 454 GRRICPGMSMATLTVELALANLLLCFDWKLPDGMKEEDVDMEEADGLAAPKKSPLRLIPV 510

BLAST of Lsi04G006180 vs. ExPASy TrEMBL
Match: A0A6J1GWF5 (cytochrome P450 71B26-like OS=Cucurbita moschata OX=3662 GN=LOC111458147 PE=3 SV=1)

HSP 1 Score: 633.3 bits (1632), Expect = 8.3e-178
Identity = 317/482 (65.77%), Postives = 376/482 (78.01%), Query Frame = 0

Query: 7   NNKKVIVSPPTPPKLPFLGHLHLITSLSHRSFSNLSKKYGPIILLKLGSIPTVVISSATA 66
           NNK   + PP PPKLP LGHLHLI SL HRS S LSKKYGP++LL+LGS+PT+VISSA A
Sbjct: 39  NNK---LLPPGPPKLPLLGHLHLIGSLPHRSLSQLSKKYGPVMLLQLGSVPTIVISSAAA 98

Query: 67  AKEVLKVHDLASCSRPQSTTGARFSYNYLDIGLAPYGDHWREVRKICVLEMLSARRVQSF 126
           A+E+ K HDLASCSRP  T   RFSYN++D+ L+PYG+ WRE+RKIC+L++ +ARRVQSF
Sbjct: 99  ARELFKFHDLASCSRPHLTGSGRFSYNFVDLNLSPYGERWRELRKICMLQLFTARRVQSF 158

Query: 127 QMIREEEIGVLLNTISQSSSSNTPINLSEKLYSLTANITARIAFGKCFRGSELDNENFQM 186
           Q IREEE+G L+N+ISQSSSS+ PI+LS+K YSLTAN+T R+AFG  F G +LDNENFQ 
Sbjct: 159 QEIREEEVGRLVNSISQSSSSSAPIDLSKKSYSLTANVTTRVAFGSIFSGGKLDNENFQH 218

Query: 187 IIRRAMAIIGSFSANDFFPRVGWIIDWISGVHTRLEKSFAELDAFFQHVVDDRIKFREIS 246
           ++RRA+A IGSFS  DF P  GWIID ++GVH RLEKSF E+DAFFQHVVD+RI F++ S
Sbjct: 219 VMRRAVAAIGSFSMTDFIPTFGWIIDRLTGVHGRLEKSFGEMDAFFQHVVDNRINFKKTS 278

Query: 247 SHNDSDDQENIVDVLLKMEKDGSEFDVVKLPRDCIKALIM-------------------- 306
                  +ENIVDVLL+ME++ SE D +K+ +DC+KALIM                    
Sbjct: 279 K-----SEENIVDVLLRMERESSESDALKVTQDCVKALIMDIFLAGVETGTTTIVWTMTE 338

Query: 307 ------VMKKLQGEIRSCVKENEAIKESDLEKLKYLKAVVKEVLRLHAPVPLLLPREAMS 366
                 VMKKLQ EIR+C+KE + +KE DLEKL+YLK VVKE LRLH PVPLLLPRE +S
Sbjct: 339 LVKNPRVMKKLQDEIRNCIKE-DLVKEIDLEKLEYLKMVVKESLRLHPPVPLLLPRETIS 398

Query: 367 HFKLNGYDIHPKTRVYVNVWAIGRDPKLWTNPEAFLPERFIGSNIDYKGQNFELLPFGSG 426
            FKLNGYDI PK  +++NVWAIGRDP+ WTNPE F PERFIGSNIDYKGQNFEL+PFGSG
Sbjct: 399 PFKLNGYDIDPKAHLHINVWAIGRDPQSWTNPEEFFPERFIGSNIDYKGQNFELIPFGSG 458

Query: 427 RRICPGMNMATIIVELALTNILLCFDWKLPNEMKEEDVDMEEEFGIAVAKKSPLQLVPVH 463
           RRIC G+NMATI VELAL N+LL FDWKLPN MKEEDVD EE  G+  AKKSPL L+PV 
Sbjct: 459 RRICAGINMATITVELALANLLLGFDWKLPNGMKEEDVDTEEAAGLGAAKKSPLMLIPVP 511

BLAST of Lsi04G006180 vs. NCBI nr
Match: XP_038892966.1 (cytochrome P450 71B37-like [Benincasa hispida])

HSP 1 Score: 773.5 bits (1996), Expect = 1.1e-219
Identity = 391/492 (79.47%), Postives = 420/492 (85.37%), Query Frame = 0

Query: 1   MKFGSHNNKKVIVSPPTPPKLPFLGHLHLITSLSHRSFSNLSKKYGPIILLKLGSIPTVV 60
           MKF   NNKKVI+ PP PPKLP LGHLHLITSL HRSF NLSKKYGP++ LKLGS+PTVV
Sbjct: 33  MKFRRQNNKKVIIHPPNPPKLPLLGHLHLITSLPHRSFCNLSKKYGPVMFLKLGSVPTVV 92

Query: 61  ISSATAAKEVLKVHDLASCSRPQSTTGARFSYNYLDIGLAPYGDHWREVRKICVLEMLSA 120
           ISSA AAKEVLKVHDLA CSRPQS  GARFSYNYLDIGLAPYG++WREVRKICVL++ + 
Sbjct: 93  ISSADAAKEVLKVHDLACCSRPQSAAGARFSYNYLDIGLAPYGEYWREVRKICVLKLFNV 152

Query: 121 RRVQSFQMIREEEIGVLLNTISQSSSSNTPINLSEKLYSLTANITARIAFGKCFRGSELD 180
           RRVQSFQMIREEEIGVLLN+ISQSSSS TPI+LSEK YSLTAN+  RIAFGK FRG ELD
Sbjct: 153 RRVQSFQMIREEEIGVLLNSISQSSSSATPIDLSEKFYSLTANMITRIAFGKSFRGGELD 212

Query: 181 NENFQMIIRRAMAIIGSFSANDFFPRVGWIIDWISGVHTRLEKSFAELDAFFQHVVDDRI 240
           NENFQ +IRRA+A +GSFSA DFFPRVGWIID ISGVH RLE SFAELDAF QHVVDDR 
Sbjct: 213 NENFQKVIRRAVAALGSFSATDFFPRVGWIIDRISGVHKRLEMSFAELDAFLQHVVDDRT 272

Query: 241 KFREISSHNDSDDQENIVDVLLKMEKDGSEFDVVKLPRDCIKALIM-------------- 300
           KFRE SSHND D QENIVDVLLKMEKDGSEFDVVKL RDCIKAL+M              
Sbjct: 273 KFRESSSHND-DYQENIVDVLLKMEKDGSEFDVVKLTRDCIKALVMDIFLGGVETGANTL 332

Query: 301 ------------VMKKLQGEIRSCVKENEAIKESDLEKLKYLKAVVKEVLRLHAPVPLLL 360
                       VMKKLQ EIRSC++EN  +KESDL+KL+YLKAVVKEVLRLH PVPLLL
Sbjct: 333 VWAMTELIKNPKVMKKLQDEIRSCIREN-VVKESDLDKLEYLKAVVKEVLRLHTPVPLLL 392

Query: 361 PREAMSHFKLNGYDIHPKTRVYVNVWAIGRDPKLWTNPEAFLPERFIGSNIDYKGQNFEL 420
           PRE MSHFKLNGYDIHPKT ++VNVWAIGRDP+ WTNPEAFLPERFIGSNIDYKGQNFEL
Sbjct: 393 PRETMSHFKLNGYDIHPKTHIHVNVWAIGRDPESWTNPEAFLPERFIGSNIDYKGQNFEL 452

Query: 421 LPFGSGRRICPGMNMATIIVELALTNILLCFDWKLPNEMKEEDVDMEEEFGIAVAKKSPL 467
           LPFG+GRRICPGM+MATI VELAL NILLCFDWKLPN MKE+DVDMEEE GIAVAKKSPL
Sbjct: 453 LPFGTGRRICPGMSMATITVELALANILLCFDWKLPNGMKEKDVDMEEESGIAVAKKSPL 512

BLAST of Lsi04G006180 vs. NCBI nr
Match: XP_004135500.2 (cytochrome P450 71B37-like [Cucumis sativus] >KAE8648691.1 hypothetical protein Csa_009174 [Cucumis sativus])

HSP 1 Score: 734.2 bits (1894), Expect = 7.1e-208
Identity = 369/487 (75.77%), Postives = 408/487 (83.78%), Query Frame = 0

Query: 11  VIVSPPTPPKLPFLGHLHLITSLSHRSFSNLSKKYGPIILLKLGSIPTVVISSATAAKEV 70
           ++  PPTPPKLPF+GHLHL+TSL HRSF +LS+KYGP++LLKLGSIPTVV+SSATAAKEV
Sbjct: 42  IVHHPPTPPKLPFIGHLHLVTSLPHRSFRHLSRKYGPVMLLKLGSIPTVVVSSATAAKEV 101

Query: 71  LKVHDLASCSRPQSTTGARFSYNYLDIGLAPYGDHWREVRKICVLEMLSARRVQSFQMIR 130
           LKVHDLASCSRP+ST  ARFSYNYLDIG APYGDHWR+VRKI VLE+ SARRVQSFQ IR
Sbjct: 102 LKVHDLASCSRPRSTANARFSYNYLDIGFAPYGDHWRKVRKISVLELFSARRVQSFQNIR 161

Query: 131 EEEIGVLLNTISQSS--SSNTPINLSEKLYSLTANITARIAFGKCFRGSELDNENFQMII 190
           EEEIGV+LN+ISQSS   SN P++LSEK YSLTAN+  RIAFGK FRG ELDNENFQ +I
Sbjct: 162 EEEIGVMLNSISQSSLLLSNDPVDLSEKFYSLTANMITRIAFGKKFRGGELDNENFQKVI 221

Query: 191 RRAMAIIGSFSANDFFPRVGWIIDWISGVHTRLEKSFAELDAFFQHVVDDRIKFRE-ISS 250
           RRAMA +GSFSA D+ P VGWIIDW+SGVH+RLE SF ELDAFFQH+VDDRI FRE  SS
Sbjct: 222 RRAMAAVGSFSAADYLPIVGWIIDWVSGVHSRLETSFNELDAFFQHIVDDRINFRESCSS 281

Query: 251 HN---DSDDQENIVDVLLKMEKDGSEFDVVKLPRDCIKALIM------------------ 310
           HN   D  DQENIVDVLLKMEK+ S++  VKL RDCIKALIM                  
Sbjct: 282 HNNNGDGYDQENIVDVLLKMEKNSSQYGEVKLTRDCIKALIMDIFLAGVETGASTLVWTM 341

Query: 311 --------VMKKLQGEIRSCVKENEAIKESDLEKLKYLKAVVKEVLRLHAPVPLLLPREA 370
                   VMKKLQ EIR+CVKEN+ +KE+DL+ L+YLKAVVKEVLRLH P PLLLPREA
Sbjct: 342 AELIKNPKVMKKLQNEIRNCVKENKMVKENDLQNLEYLKAVVKEVLRLHTPAPLLLPREA 401

Query: 371 MSHFKLNGYDIHPKTRVYVNVWAIGRDPKLWTNPEAFLPERFIGSNIDYKGQNFELLPFG 430
           MSHFKLNGYDI PKT +YVN WAIGRDP++WTNPE F+PERFIGSNIDYKGQNFELLPFG
Sbjct: 402 MSHFKLNGYDILPKTHIYVNAWAIGRDPEIWTNPEEFIPERFIGSNIDYKGQNFELLPFG 461

Query: 431 SGRRICPGMNMATIIVELALTNILLCFDWKLPNEMKEEDVDMEEEFGIAVAKKSPLQLVP 466
           SGRRICPGMNMA+  VELAL N+LLCFDWKL N MKEEDVDMEEE G+AVAKKSPLQLVP
Sbjct: 462 SGRRICPGMNMASFTVELALANVLLCFDWKLANGMKEEDVDMEEETGLAVAKKSPLQLVP 521

BLAST of Lsi04G006180 vs. NCBI nr
Match: KAA0034250.1 (cytochrome P450 71B37-like [Cucumis melo var. makuwa] >TYK15670.1 cytochrome P450 71B37-like [Cucumis melo var. makuwa])

HSP 1 Score: 729.6 bits (1882), Expect = 1.7e-206
Identity = 364/488 (74.59%), Postives = 406/488 (83.20%), Query Frame = 0

Query: 8   NKKVIVSPPTPPKLPFLGHLHLITSLSHRSFSNLSKKYGPIILLKLGSIPTVVISSATAA 67
           N  ++  PPTPPKLPFLGHLHLITSLSHRSF +LS++YGP++LL+LGSIPTVV+SSA AA
Sbjct: 4   NNVIVHLPPTPPKLPFLGHLHLITSLSHRSFRHLSEEYGPVMLLQLGSIPTVVVSSAAAA 63

Query: 68  KEVLKVHDLASCSRPQSTTGARFSYNYLDIGLAPYGDHWREVRKICVLEMLSARRVQSFQ 127
           KEVLKVHDLASCSRP+S   ARFSYNYLDIGLAPYG+HWREVRKICVLE+ SARRVQSFQ
Sbjct: 64  KEVLKVHDLASCSRPRSVANARFSYNYLDIGLAPYGEHWREVRKICVLELFSARRVQSFQ 123

Query: 128 MIREEEIGVLLNTISQSS-SSNTPINLSEKLYSLTANITARIAFGKCFRGSELDNENFQM 187
            IREEEIGV+LN+ISQSS  SN PI+LSEK YSLTANI  RIAFGK FRG ELDNENFQ 
Sbjct: 124 KIREEEIGVMLNSISQSSLLSNDPIDLSEKFYSLTANIITRIAFGKKFRGGELDNENFQK 183

Query: 188 IIRRAMAIIGSFSANDFFPRVGWIIDWISGVHTRLEKSFAELDAFFQHVVDDRIKFRE-- 247
           +IRRAMA +GS SA D+FP+VGWIIDW++GVH RLE SF ELD FFQH+VDDRIKFRE  
Sbjct: 184 VIRRAMAAVGSSSAADYFPKVGWIIDWVNGVHRRLEMSFNELDIFFQHIVDDRIKFRESP 243

Query: 248 -ISSHNDSDDQENIVDVLLKMEKDGSEFDVVKLPRDCIKALIM----------------- 307
             S  ND D+QENIVDVLLKMEK+ S+F  +KL RDCIKALIM                 
Sbjct: 244 RSSQDNDDDEQENIVDVLLKMEKNSSQFGEMKLTRDCIKALIMDIFLAGVETGATTLVWA 303

Query: 308 ---------VMKKLQGEIRSCVKENEAIKESDLEKLKYLKAVVKEVLRLHAPVPLLLPRE 367
                    VMKKLQ EIR+CVKE+  +KESDL+ L+YLKAVVKEVLRLH P PLLLPRE
Sbjct: 304 MTELIKNPKVMKKLQNEIRNCVKESTIVKESDLQNLEYLKAVVKEVLRLHTPAPLLLPRE 363

Query: 368 AMSHFKLNGYDIHPKTRVYVNVWAIGRDPKLWTNPEAFLPERFIGSNIDYKGQNFELLPF 427
           AMSHFKLNGYDI PKT ++VNVWAIGRD ++WTNPE F+PERFIGSNIDYKGQNFE LPF
Sbjct: 364 AMSHFKLNGYDILPKTHIHVNVWAIGRDSEIWTNPEEFIPERFIGSNIDYKGQNFEFLPF 423

Query: 428 GSGRRICPGMNMATIIVELALTNILLCFDWKLPNEMKEEDVDMEEEFGIAVAKKSPLQLV 466
           GSGRRICPGMNMA+  VELAL N+LLCFDWKLPN +KEED+DMEEE G+  +KKSPLQLV
Sbjct: 424 GSGRRICPGMNMASFTVELALANMLLCFDWKLPNGVKEEDIDMEEELGLTASKKSPLQLV 483

BLAST of Lsi04G006180 vs. NCBI nr
Match: XP_016900197.1 (PREDICTED: cytochrome P450 71B37-like [Cucumis melo])

HSP 1 Score: 729.6 bits (1882), Expect = 1.7e-206
Identity = 364/488 (74.59%), Postives = 406/488 (83.20%), Query Frame = 0

Query: 8   NKKVIVSPPTPPKLPFLGHLHLITSLSHRSFSNLSKKYGPIILLKLGSIPTVVISSATAA 67
           N  ++  PPTPPKLPFLGHLHLITSLSHRSF +LS++YGP++LL+LGSIPTVV+SSA AA
Sbjct: 57  NNVIVHLPPTPPKLPFLGHLHLITSLSHRSFRHLSEEYGPVMLLQLGSIPTVVVSSAAAA 116

Query: 68  KEVLKVHDLASCSRPQSTTGARFSYNYLDIGLAPYGDHWREVRKICVLEMLSARRVQSFQ 127
           KEVLKVHDLASCSRP+S   ARFSYNYLDIGLAPYG+HWREVRKICVLE+ SARRVQSFQ
Sbjct: 117 KEVLKVHDLASCSRPRSVANARFSYNYLDIGLAPYGEHWREVRKICVLELFSARRVQSFQ 176

Query: 128 MIREEEIGVLLNTISQSS-SSNTPINLSEKLYSLTANITARIAFGKCFRGSELDNENFQM 187
            IREEEIGV+LN+ISQSS  SN PI+LSEK YSLTANI  RIAFGK FRG ELDNENFQ 
Sbjct: 177 KIREEEIGVMLNSISQSSLLSNDPIDLSEKFYSLTANIITRIAFGKKFRGGELDNENFQK 236

Query: 188 IIRRAMAIIGSFSANDFFPRVGWIIDWISGVHTRLEKSFAELDAFFQHVVDDRIKFRE-- 247
           +IRRAMA +GS SA D+FP+VGWIIDW++GVH RLE SF ELD FFQH+VDDRIKFRE  
Sbjct: 237 VIRRAMAAVGSSSAADYFPKVGWIIDWVNGVHRRLEMSFNELDIFFQHIVDDRIKFRESP 296

Query: 248 -ISSHNDSDDQENIVDVLLKMEKDGSEFDVVKLPRDCIKALIM----------------- 307
             S  ND D+QENIVDVLLKMEK+ S+F  +KL RDCIKALIM                 
Sbjct: 297 RSSQDNDDDEQENIVDVLLKMEKNSSQFGEMKLTRDCIKALIMDIFLAGVETGATTLVWA 356

Query: 308 ---------VMKKLQGEIRSCVKENEAIKESDLEKLKYLKAVVKEVLRLHAPVPLLLPRE 367
                    VMKKLQ EIR+CVKE+  +KESDL+ L+YLKAVVKEVLRLH P PLLLPRE
Sbjct: 357 MTELIKNPKVMKKLQNEIRNCVKESTIVKESDLQNLEYLKAVVKEVLRLHTPAPLLLPRE 416

Query: 368 AMSHFKLNGYDIHPKTRVYVNVWAIGRDPKLWTNPEAFLPERFIGSNIDYKGQNFELLPF 427
           AMSHFKLNGYDI PKT ++VNVWAIGRD ++WTNPE F+PERFIGSNIDYKGQNFE LPF
Sbjct: 417 AMSHFKLNGYDILPKTHIHVNVWAIGRDSEIWTNPEEFIPERFIGSNIDYKGQNFEFLPF 476

Query: 428 GSGRRICPGMNMATIIVELALTNILLCFDWKLPNEMKEEDVDMEEEFGIAVAKKSPLQLV 466
           GSGRRICPGMNMA+  VELAL N+LLCFDWKLPN +KEED+DMEEE G+  +KKSPLQLV
Sbjct: 477 GSGRRICPGMNMASFTVELALANMLLCFDWKLPNGVKEEDIDMEEELGLTASKKSPLQLV 536

BLAST of Lsi04G006180 vs. NCBI nr
Match: XP_023542551.1 (cytochrome P450 71B26-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 641.3 bits (1653), Expect = 6.3e-180
Identity = 320/490 (65.31%), Postives = 385/490 (78.57%), Query Frame = 0

Query: 2   KFGSHNNKKVIVSPPTPPKLPFLGHLHLITSLSHRSFSNLSKKYGPIILLKLGSIPTVVI 61
           K    NNK   + PPTPPKLP LGHLHLI SL HRS S LSKKYGP++LL+LGS+PT+VI
Sbjct: 28  KHSKPNNK---LLPPTPPKLPLLGHLHLIGSLPHRSLSQLSKKYGPVMLLQLGSVPTIVI 87

Query: 62  SSATAAKEVLKVHDLASCSRPQSTTGARFSYNYLDIGLAPYGDHWREVRKICVLEMLSAR 121
           SSA AA+++ K HDLASCSRP  T   RFSYN+LD+ L+PYG+ WRE+RKIC+L++ +AR
Sbjct: 88  SSAAAARDLFKFHDLASCSRPLLTGSGRFSYNFLDLNLSPYGERWRELRKICMLQLFTAR 147

Query: 122 RVQSFQMIREEEIGVLLNTISQSSSSNTPINLSEKLYSLTANITARIAFGKCFRGSELDN 181
           RVQSFQ IREEE+G L+N+ISQSSSS+ PI+LS+K YSLTAN+T R+AFG  F G +LDN
Sbjct: 148 RVQSFQEIREEEVGRLVNSISQSSSSSAPIDLSKKSYSLTANVTTRVAFGSIFSGGKLDN 207

Query: 182 ENFQMIIRRAMAIIGSFSANDFFPRVGWIIDWISGVHTRLEKSFAELDAFFQHVVDDRIK 241
           ENFQ ++RRA+A IGSFS  DF P  GWIID ++GVH RLEKSFAE+DAFFQHVVDDRI 
Sbjct: 208 ENFQHVMRRAVAAIGSFSMTDFIPTFGWIIDRLNGVHERLEKSFAEMDAFFQHVVDDRIN 267

Query: 242 FREISSHNDSDDQENIVDVLLKMEKDGSEFDVVKLPRDCIKALIM--------------- 301
           F+E      S ++ENIVDVLL+ME++ SE D +K+ +DC+KALIM               
Sbjct: 268 FKE-----SSKNEENIVDVLLRMERESSESDALKVTQDCVKALIMDIFLAGVETGATTIV 327

Query: 302 -----------VMKKLQGEIRSCVKENEAIKESDLEKLKYLKAVVKEVLRLHAPVPLLLP 361
                      VMKKLQ E+RS VKE +++KE DLEKL+YLK VVKE LRLH PVPLLLP
Sbjct: 328 RTMTELVRNPRVMKKLQHEVRSYVKE-DSVKEIDLEKLEYLKMVVKESLRLHPPVPLLLP 387

Query: 362 REAMSHFKLNGYDIHPKTRVYVNVWAIGRDPKLWTNPEAFLPERFIGSNIDYKGQNFELL 421
           RE +S FKLNGYDI PK  +++NVWAIGRDP+ WT+PE F PERFIGSNIDYKGQNFEL+
Sbjct: 388 RETISPFKLNGYDIDPKAHLHINVWAIGRDPQYWTDPEEFFPERFIGSNIDYKGQNFELI 447

Query: 422 PFGSGRRICPGMNMATIIVELALTNILLCFDWKLPNEMKEEDVDMEEEFGIAVAKKSPLQ 466
           PFGSGRRIC GMNMAT++VELAL N+LLCFDWKLP+ MKEEDVD EE  G+  AKKSPL+
Sbjct: 448 PFGSGRRICAGMNMATLVVELALANLLLCFDWKLPDGMKEEDVDREEGAGLGSAKKSPLK 507

BLAST of Lsi04G006180 vs. TAIR 10
Match: AT3G26330.1 (cytochrome P450, family 71, subfamily B, polypeptide 37 )

HSP 1 Score: 466.8 bits (1200), Expect = 2.0e-131
Identity = 233/475 (49.05%), Postives = 312/475 (65.68%), Query Frame = 0

Query: 15  PPTPPKLPFLGHLHLITSLSHRSFSNLSKKYGPIILLKLGSIPTVVISSATAAKEVLKVH 74
           PP+PP  P +G+LH +  L H+S  +LSKKYGP++LLK GSIPTVV+SS+  AK+ LK+H
Sbjct: 32  PPSPPGFPIIGNLHQLGELPHQSLWSLSKKYGPVMLLKFGSIPTVVVSSSETAKQALKIH 91

Query: 75  DLASCSRPQSTTGARFSYNYLDIGLAPYGDHWREVRKICVLEMLSARRVQSFQMIREEEI 134
           DL  CSRP        SYNYLDI  +P+ D+W+E+R++CV E+ S ++V   Q IREEE+
Sbjct: 92  DLNCCSRPSLAGPRALSYNYLDIVFSPFNDYWKELRRMCVQELFSPKQVHLIQPIREEEV 151

Query: 135 GVLLNTISQSSSSNTPINLSEKLYSLTANITARIAFGKCFRGSELDNENFQMIIRRAMAI 194
             L+N+ S+S++  TP+NLSEKL SLT  +  + AFG  F+G+ L+++NF  +I  A   
Sbjct: 152 KKLMNSFSESAAQKTPVNLSEKLASLTVGVICKAAFGVSFQGTVLNSDNFDKLIHDAFLF 211

Query: 195 IGSFSANDFFPRVGWIIDWISGVHTRLEKSFAELDAFFQHVVDDRIKFREISSHNDSDDQ 254
           +GSFSA+D+FP VGWIIDW++G+  + E+S   LDAF++ + D       +    + +  
Sbjct: 212 LGSFSASDYFPNVGWIIDWLTGLQGQRERSVRGLDAFYEQMFD-------LHKQGNKEGV 271

Query: 255 ENIVDVLLKMEKDGSEFDVVKLPRDCIKALIM--------------------------VM 314
           E+ VD+LLK+EK+ +     KL R+ IKA++M                          VM
Sbjct: 272 EDFVDLLLKLEKEETVLGYGKLTRNHIKAVLMNVLLGGIGTSAITMTWAMTELMRNPRVM 331

Query: 315 KKLQGEIRSCVKENEAIKESDLEKLKYLKAVVKEVLRLHAPVPLLLPREAMSHFKLNGYD 374
           KK+Q EIR+ +     I   D+++L YLK V+ E  RLH P PLL+PRE MS F++NGY 
Sbjct: 332 KKVQSEIRNQIGGKSMICLDDIDQLHYLKMVINETWRLHPPAPLLVPREVMSEFEINGYT 391

Query: 375 IHPKTRVYVNVWAIGRDPKLWTNPEAFLPERFIGSNIDYKGQNFELLPFGSGRRICPGMN 434
           I  KTR+YVNVW IGRDP  W +PE FLPERF+ SNID KGQNFELLPFGSGRR+CP M 
Sbjct: 392 IPAKTRLYVNVWGIGRDPDTWKDPEEFLPERFVNSNIDAKGQNFELLPFGSGRRMCPAMY 451

Query: 435 MATIIVELALTNILLCFDWKLPNEMKEEDVDMEEEFGIAVAKKSPLQLVPVHYFN 464
           M T +VE  L N+L  FDWKLP  M  ED+DMEE  G+  +KK+ L LVP  Y N
Sbjct: 452 MGTTMVEFGLANLLYHFDWKLPEGMVVEDIDMEESPGLNASKKNELVLVPRKYLN 499

BLAST of Lsi04G006180 vs. TAIR 10
Match: AT2G02580.1 (cytochrome P450, family 71, subfamily B, polypeptide 9 )

HSP 1 Score: 461.8 bits (1187), Expect = 6.3e-130
Identity = 232/473 (49.05%), Postives = 310/473 (65.54%), Query Frame = 0

Query: 15  PPTPPKLPFLGHLHLITSLSHRSFSNLSKKYGPIILLKLGSIPTVVISSATAAKEVLKVH 74
           PP+PP  P +G+LH +  L H+S  +LSK YGP++LLKLGS+PTVV+SS+  AK+VLK++
Sbjct: 32  PPSPPGFPIIGNLHQLGELPHQSLWSLSKTYGPVMLLKLGSVPTVVVSSSETAKQVLKIN 91

Query: 75  DLASCSRPQSTTGARFSYNYLDIGLAPYGDHWREVRKICVLEMLSARRVQSFQMIREEEI 134
           DL  CSRP        SYNYLDI  +P+ D+W+E+R+ICV E+ SA+RV S Q I+EEE+
Sbjct: 92  DLHCCSRPSLAGAKELSYNYLDIAFSPFDDYWKELRRICVQELFSAKRVHSIQPIKEEEV 151

Query: 135 GVLLNTISQSSSSNTPINLSEKLYSLTANITARIAFGKCFRGSELDNENFQMIIRRAMAI 194
             L+ + ++S+S  +P+NLSEK   LT ++  + AF   F  S L+N+ F  +I  A   
Sbjct: 152 RKLIVSATESASQKSPVNLSEKFLDLTVSVICKAAFSLDFHTSVLNNDGFDKLIHDAFLF 211

Query: 195 IGSFSANDFFPRVGWIIDWISGVHTRLEKSFAELDAFFQHVVDDRIKFREISSHNDSDDQ 254
           +GSFSA++FFP  GWIIDW++G+  R EKS  +LD F+Q + D       +    +    
Sbjct: 212 LGSFSASNFFPNGGWIIDWLTGLQRRREKSVKDLDVFYQQMFD-------LHKQENKQGV 271

Query: 255 ENIVDVLLKMEKDGSEFDVVKLPRDCIKALIM--------------------------VM 314
           E+ VD+LLK+EK+ +     KL R+ +KA++M                          VM
Sbjct: 272 EDFVDLLLKLEKEETVLGYGKLTRNHVKAILMNVLLGAINTSAMTMTWAMAELIRNPRVM 331

Query: 315 KKLQGEIRSCVKENEAIKESDLEKLKYLKAVVKEVLRLHAPVPLLLPREAMSHFKLNGYD 374
           KK+Q EIR+ +     I   D++ L YLK V+KE  RLH PVPLLLPRE MS F++NGY 
Sbjct: 332 KKVQSEIRNQMINKSVITLDDIDHLPYLKMVIKETWRLHPPVPLLLPREVMSEFEINGYK 391

Query: 375 IHPKTRVYVNVWAIGRDPKLWTNPEAFLPERFIGSNIDYKGQNFELLPFGSGRRICPGMN 434
           I PKT +YVNVWAIGRDP  W + + F PERF+ +NID KGQNFELLPFGSGRRICPGM 
Sbjct: 392 IQPKTLLYVNVWAIGRDPDSWKDADMFYPERFMDNNIDAKGQNFELLPFGSGRRICPGMY 451

Query: 435 MATIIVELALTNILLCFDWKLPNEMKEEDVDMEEEFGIAVAKKSPLQLVPVHY 462
           M T +VE  L N+L  FDW++P+ M  ED+DMEE  G+AV KK+ L LVPV Y
Sbjct: 452 MGTTMVEFGLANMLYQFDWEVPDGMVVEDIDMEESPGLAVGKKNELLLVPVKY 497

BLAST of Lsi04G006180 vs. TAIR 10
Match: AT3G26320.1 (cytochrome P450, family 71, subfamily B, polypeptide 36 )

HSP 1 Score: 458.0 bits (1177), Expect = 9.1e-129
Identity = 226/475 (47.58%), Postives = 314/475 (66.11%), Query Frame = 0

Query: 15  PPTPPKLPFLGHLHLITSLSHRSFSNLSKKYGPIILLKLGSIPTVVISSATAAKEVLKVH 74
           PP+PP  P +G+LH +  L H+S   LSKKYG ++LLK GSIPTVV+SS+  AK+VLK+H
Sbjct: 32  PPSPPGFPIIGNLHQLGELPHQSLWRLSKKYGHVMLLKFGSIPTVVVSSSETAKQVLKIH 91

Query: 75  DLASCSRPQSTTGARFSYNYLDIGLAPYGDHWREVRKICVLEMLSARRVQSFQMIREEEI 134
           DL  CSRP        SYNYLDI  +P+ D+W+E+R+ICV E+ S +RVQSFQ I+E+E+
Sbjct: 92  DLHCCSRPSLAGPRALSYNYLDIAFSPFDDYWKELRRICVQELFSVKRVQSFQPIKEDEV 151

Query: 135 GVLLNTISQSSSSNTPINLSEKLYSLTANITARIAFGKCFRGSELDNENFQMIIRRAMAI 194
             L++++S+S+S  TP+NLSEK  SLT  +T +  FG  F+G+ L+++ F+ +I      
Sbjct: 152 KKLIDSVSESASQGTPVNLSEKFTSLTVRVTCKATFGVNFQGTVLNSDRFEKLIHDTYLF 211

Query: 195 IGSFSANDFFPRVGWIIDWISGVHTRLEKSFAELDAFFQHVVDDRIKFREISSHNDSDDQ 254
           +GSFSA+D+FP  GWIIDW++G+H + E+S   LDAF++ + D       +    + +  
Sbjct: 212 LGSFSASDYFPNGGWIIDWLTGLHGQRERSVRALDAFYEQMFD-------LHKQGNKEGV 271

Query: 255 ENIVDVLLKMEKDGSEFDVVKLPRDCIKALIM--------------------------VM 314
           E+ VD+LL++EK+ +     KL R+ IKA++M                          VM
Sbjct: 272 EDFVDLLLRLEKEETVIGYGKLTRNHIKAILMNVLIGGIGTSAITMTWAMTELMRNPRVM 331

Query: 315 KKLQGEIRSCVKENEAIKESDLEKLKYLKAVVKEVLRLHAPVPLLLPREAMSHFKLNGYD 374
           KK+Q EIR+ + +   I   D+++L YLK V+ E  RLH P P L+PR+ MS F+LN Y 
Sbjct: 332 KKVQSEIRNQIGKKSMITLDDIDQLHYLKMVINETWRLHPPSPFLIPRQVMSEFELNDYV 391

Query: 375 IHPKTRVYVNVWAIGRDPKLWTNPEAFLPERFIGSNIDYKGQNFELLPFGSGRRICPGMN 434
           I  KTR+YVNVWAIGRDP  W +PE FLPERF+ S+ID KGQ+FELLPFGSGRR+CP M 
Sbjct: 392 IPVKTRLYVNVWAIGRDPDTWKDPEEFLPERFVNSSIDAKGQHFELLPFGSGRRMCPAMY 451

Query: 435 MATIIVELALTNILLCFDWKLPNEMKEEDVDMEEEFGIAVAKKSPLQLVPVHYFN 464
           M T +VE  L N+L  FDWK+P  M  ED+D+EE  G+  +KK+ L LVP+ Y +
Sbjct: 452 MGTTMVEFGLANMLYHFDWKIPVGMVAEDIDLEESPGLNASKKNELVLVPLKYLD 499

BLAST of Lsi04G006180 vs. TAIR 10
Match: AT3G26300.1 (cytochrome P450, family 71, subfamily B, polypeptide 34 )

HSP 1 Score: 456.4 bits (1173), Expect = 2.7e-128
Identity = 230/487 (47.23%), Postives = 317/487 (65.09%), Query Frame = 0

Query: 3   FGSHNNKKVIVSPPTPPKLPFLGHLHLITSLSHRSFSNLSKKYGPIILLKLGSIPTVVIS 62
           F   N +    +PP+PP  P +G+LH +  L H+S   LSKKYGP++LLKLG +PTV++S
Sbjct: 20  FNHKNRRNYQRTPPSPPGCPIIGNLHQLGELPHQSLWKLSKKYGPVMLLKLGRVPTVIVS 79

Query: 63  SATAAKEVLKVHDLASCSRPQSTTGARFSYNYLDIGLAPYGDHWREVRKICVLEMLSARR 122
           S+  AK+ LK+HDL  CSRP        SYNYLDI  +PY D+W+EVRK+ V E+ S+++
Sbjct: 80  SSETAKQALKIHDLHCCSRPGFAGARELSYNYLDIAFSPYDDYWKEVRKLAVQELFSSKQ 139

Query: 123 VQSFQMIREEEIGVLLNTISQSSSSNTPINLSEKLYSLTANITARIAFGKCFRGSELDNE 182
           V S Q I++EE+  L+++IS+S++  TPINL++ L +LT ++  R AF   F G+ L++E
Sbjct: 140 VHSIQPIKDEEVKKLIDSISESAAQKTPINLNKTLLALTVSVVCRTAFSVNFEGTVLNSE 199

Query: 183 NFQMIIRRAMAIIGSFSANDFFPRVGWIIDWISGVHTRLEKSFAELDAFFQHVVDDRIKF 242
            F  I+R A+ ++GSFSA+DF P VG IID ++G+  R E+S  +LDAF++ + D     
Sbjct: 200 RFNNIVREALEMLGSFSASDFIPYVGRIIDLLTGLQGRRERSMRDLDAFYEQMFD----- 259

Query: 243 REISSHNDSDDQENIVDVLLKMEKDGSEFDVVKLPRDCIKALIM---------------- 302
             +      +  E+ VD+LL++EK+ +     KL R+ IKA++M                
Sbjct: 260 --LHKQKKEEGSEDFVDLLLRLEKEEAVLGNDKLTRNHIKAILMDVLLAGMDTSAITMTW 319

Query: 303 ----------VMKKLQGEIRSCVKENEAIKESDLEKLKYLKAVVKEVLRLHAPVPLLLPR 362
                     VMKK+Q EIRS +K  E I   D +KL+YLK V+KE  RLH   PLL+PR
Sbjct: 320 AMAELAKNPRVMKKVQSEIRSQIKNKERISFDDTDKLEYLKMVIKETWRLHPTTPLLIPR 379

Query: 363 EAMSHFKLNGYDIHPKTRVYVNVWAIGRDPKLWTNPEAFLPERFIGSNIDYKGQNFELLP 422
           EAMS F++NGY I  KTR++VNVWAIGRDP  W +PE FLPERF  +NID KGQ+FELLP
Sbjct: 380 EAMSEFEINGYTIPVKTRLHVNVWAIGRDPDTWKDPEVFLPERFTDNNIDAKGQHFELLP 439

Query: 423 FGSGRRICPGMNMATIIVELALTNILLCFDWKLPNEMKEEDVDMEEEFGIAVAKKSPLQL 464
           FG GRR+CP + M T +VE  L N+L  FDWKLP  MK +D+DMEE  G+ V KK+ L L
Sbjct: 440 FGGGRRMCPAVYMGTTMVEFGLANLLYHFDWKLPEGMKVDDIDMEEAPGLTVNKKNELIL 499

BLAST of Lsi04G006180 vs. TAIR 10
Match: AT3G26290.1 (cytochrome P450, family 71, subfamily B, polypeptide 26 )

HSP 1 Score: 451.1 bits (1159), Expect = 1.1e-126
Identity = 226/474 (47.68%), Postives = 313/474 (66.03%), Query Frame = 0

Query: 16  PTPPKLPFLGHLHLITSLSHRSFSNLSKKYGPIILLKLGSIPTVVISSATAAKEVLKVHD 75
           P+PP  P +G+LH +  L H+S   LSKKYGP++LLKLG +PT+++SS+  AK+ L+ +D
Sbjct: 33  PSPPGFPIIGNLHQLGELQHQSLWKLSKKYGPVMLLKLGKVPTLILSSSETAKQALRDYD 92

Query: 76  LASCSRPQSTTGARFSYNYLDIGLAPYGDHWREVRKICVLEMLSARRVQSFQMIREEEIG 135
           L  CSRP    G   SYN LD+  +PY ++W+E+RK+C  E+ SA ++QS Q I++EE+ 
Sbjct: 93  LHCCSRPSLAGGRELSYNNLDMSSSPYNEYWKELRKLCSQELFSANKIQSIQPIKDEEVK 152

Query: 136 VLLNTISQSSSSNTPINLSEKLYSLTANITARIAFGKCFRGSELDNENFQMIIRRAMAII 195
            ++++I++SSS   P+NLS+   +LT ++  + AFG  F GS L+++ F  ++R    ++
Sbjct: 153 KVIDSIAESSSLKNPVNLSKTFLALTTSVVCKAAFGVSFEGSVLNSDRFNKLVRDTFEML 212

Query: 196 GSFSANDFFPRVGWIIDWISGVHTRLEKSFAELDAFFQHVVDDRIKFREISSHNDSDDQE 255
           GSFSA+DF P VGWIID  +G+    +KSF +LDAF++ + D   + +E+ S       E
Sbjct: 213 GSFSASDFIPYVGWIIDKFNGLQGWRKKSFRDLDAFYEQIFDLHKEEKEVGS-------E 272

Query: 256 NIVDVLLKMEKDGSEFDVVKLPRDCIKALIM--------------------------VMK 315
           ++VDVLL++EK+       KL R+ IKA++M                          VMK
Sbjct: 273 DLVDVLLRLEKEEIVVGNGKLTRNHIKAILMNILLGGIDTSAITMTWAMAELAKNPRVMK 332

Query: 316 KLQGEIRSCVKENEAIKESDLEKLKYLKAVVKEVLRLHAPVPLLLPREAMSHFKLNGYDI 375
           K+Q EIR+ +K  E I   D +KL+YLK V+KE  RLH P PLLLPR+ ++ F++NGY I
Sbjct: 333 KVQAEIRNQIKNKERISFDDTDKLEYLKMVIKETWRLHPPTPLLLPRDVITEFEINGYTI 392

Query: 376 HPKTRVYVNVWAIGRDPKLWTNPEAFLPERFIGSNIDYKGQNFELLPFGSGRRICPGMNM 435
             KTR++VNVWAIGRDP  W +PE FLPERF  SNID KGQNFELL FGSGRRICPG+ M
Sbjct: 393 PAKTRLHVNVWAIGRDPDTWKDPEMFLPERFNDSNIDAKGQNFELLSFGSGRRICPGLYM 452

Query: 436 ATIIVELALTNILLCFDWKLPNEMKEEDVDMEEEFGIAVAKKSPLQLVPVHYFN 464
            T +VE  L N+L  FDWKLP  M  ED+DMEE  G+ V+KKS L LVPV Y +
Sbjct: 453 GTTMVEFGLANMLYHFDWKLPEGMVVEDIDMEEAPGLTVSKKSELVLVPVKYLD 499

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LIP32.8e-13049.05Cytochrome P450 71B37 OS=Arabidopsis thaliana OX=3702 GN=CYP71B37 PE=3 SV=2[more]
O647188.9e-12949.05Cytochrome P450 71B9 OS=Arabidopsis thaliana OX=3702 GN=CYP71B9 PE=2 SV=3[more]
Q9LIP41.3e-12747.58Cytochrome P450 71B36 OS=Arabidopsis thaliana OX=3702 GN=CYP71B36 PE=3 SV=1[more]
Q9LIP63.7e-12747.23Cytochrome P450 71B34 OS=Arabidopsis thaliana OX=3702 GN=CYP71B34 PE=2 SV=1[more]
Q9LTL01.6e-12547.68Cytochrome P450 71B26 OS=Arabidopsis thaliana OX=3702 GN=CYP71B26 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A5D3CWC38.5e-20774.59Cytochrome P450 71B37-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaff... [more]
A0A1S4DW348.5e-20774.59cytochrome P450 71B37-like OS=Cucumis melo OX=3656 GN=LOC103488948 PE=3 SV=1[more]
A0A6J1GWP78.3e-17865.22cytochrome P450 71B26-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111458... [more]
A0A6J1GW768.3e-17865.22cytochrome P450 71B26-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111458... [more]
A0A6J1GWF58.3e-17865.77cytochrome P450 71B26-like OS=Cucurbita moschata OX=3662 GN=LOC111458147 PE=3 SV... [more]
Match NameE-valueIdentityDescription
XP_038892966.11.1e-21979.47cytochrome P450 71B37-like [Benincasa hispida][more]
XP_004135500.27.1e-20875.77cytochrome P450 71B37-like [Cucumis sativus] >KAE8648691.1 hypothetical protein ... [more]
KAA0034250.11.7e-20674.59cytochrome P450 71B37-like [Cucumis melo var. makuwa] >TYK15670.1 cytochrome P45... [more]
XP_016900197.11.7e-20674.59PREDICTED: cytochrome P450 71B37-like [Cucumis melo][more]
XP_023542551.16.3e-18065.31cytochrome P450 71B26-like isoform X2 [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
AT3G26330.12.0e-13149.05cytochrome P450, family 71, subfamily B, polypeptide 37 [more]
AT2G02580.16.3e-13049.05cytochrome P450, family 71, subfamily B, polypeptide 9 [more]
AT3G26320.19.1e-12947.58cytochrome P450, family 71, subfamily B, polypeptide 36 [more]
AT3G26300.12.7e-12847.23cytochrome P450, family 71, subfamily B, polypeptide 34 [more]
AT3G26290.11.1e-12647.68cytochrome P450, family 71, subfamily B, polypeptide 26 [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 288..308
NoneNo IPR availablePANTHERPTHR47956:SF5CYTOCHROME P450 71B16-RELATEDcoord: 287..461
coord: 10..286
NoneNo IPR availablePANTHERPTHR47956CYTOCHROME P450 71B11-RELATEDcoord: 287..461
coord: 10..286
IPR002401Cytochrome P450, E-class, group IPRINTSPR00463EP450Icoord: 404..427
score: 31.8
coord: 317..335
score: 41.52
coord: 42..61
score: 42.75
coord: 394..404
score: 56.8
coord: 358..382
score: 38.96
coord: 66..87
score: 24.4
coord: 160..178
score: 25.51
IPR036396Cytochrome P450 superfamilyGENE3D1.10.630.10Cytochrome P450coord: 2..284
e-value: 3.3E-46
score: 159.8
IPR036396Cytochrome P450 superfamilyGENE3D1.10.630.10Cytochrome P450coord: 285..460
e-value: 2.7E-57
score: 196.4
IPR036396Cytochrome P450 superfamilySUPERFAMILY48264Cytochrome P450coord: 15..458
IPR001128Cytochrome P450PFAMPF00067p450coord: 287..449
e-value: 6.7E-51
score: 173.5
coord: 15..286
e-value: 2.1E-28
score: 99.3
IPR017972Cytochrome P450, conserved sitePROSITEPS00086CYTOCHROME_P450coord: 397..406

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi04G006180.1Lsi04G006180.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0020037 heme binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0004497 monooxygenase activity
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen