Lsi04G003350 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi04G003350
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionUDP-glycosyltransferase 89A2-like
Locationchr04: 3399716 .. 3401134 (+)
RNA-Seq ExpressionLsi04G003350
SyntenyLsi04G003350
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCGTCTTCACCGCCGTCGTCCGCCACCTCCCGCCACCTCCTGGTTTTTCCATACCCAGCTCAGGGACATATGATCGCACTCCTCGATCTCACCCATCACTTAGCCTCTCATGGCGGCTTCACAATTACCCTTTTGGTTACCCCCAAAAACCTCCATCTCCTTCAGCCCCTTCTTCAAACCCACCCTTCTGTTCAAACCCTCGTTCTCCCCTTACCTTCTCACCCCAAACTCCCCGTCGGCGTCGAACACGTCGCCCACATCGGCAACCATGGCAATTTCCCGATCATGGCTGCCCTCCGCCAGCTCCACGACCCCATTGTTGAGTGGTTCAACTCTCATCCTTCCCCTCCCGTCGCCATCATTTCCGATTTCTTTCTCGGCTGGACCCAACGTCTCGCCGACCATCTTCGAATTCCCAGAGTTGTTTTCCACTCCATTGCTACATTACTCGTCCATGTAATGAACTCTTGTTGGGTTCAAATTAAAACAGATCATTTTCGTTCTTCCCCTGTCATACAGTTCAGTGAAATCCCCAAATCGCCTTCCTTCAGAAAAGAGCATTTGCCATCGTTGGTAAGGCTGTACCAGGATTCGGACCCCGATTGGAATTTATTGAGGGACGACGCCTTAGGTAATACATCCAGCTGGGCTTGTGTTGTTAATACGTTCGATAGTTTGGAGCACGAGTATTTAGAGCACTTGAGGAAATTGAGGGGCGAAGGGCGTGTATTTGGTATCGGACCGCTTGATATCATCGGCGCTGCAGAGGATGGGCGAAACCCAATTCGAGAGTCGAGTTCAGAGATTCTCACATGGCTGGACAGATGCCCTGATGATTCGGTGCTTTACGTTTGTTTTGGGAGTCAAAAGCAACTGAATCAGCAGCAAATGGAAGCACTGGCTTCCGGGCTTGAGAAAAGCGGTACTCGATTCGTGTGGGTGGTTAAGACATTTCATCAAACAGATGGTGGGTCGGACGGAATACCAATTGGGTTTGAGGATCGGGTGTCAGATCGTGGAATTTTGGCGAAAGGATGGGCGCCGCAAGTGTCAATACTGAACCACCGAGCCGTGGGGGGGTTTCTGAGTCACTGTGGGTGGAACTCGGTGCTTGAGAGCATAGTGGGCGGAGTAATGATACTTGGTTGGCCCATGGAAGCAGACCAGTTTATCAATGCGAGGCTATTGGTAGAAGAGTTGGGAGTAGCGGTGCGCGTTTGCGAGGGGGCGAACTCGGTGCCTGAATCGGACGAGTTGGGGAAGGTCATCGCCGAGTCTTTGAGCGGAGATTCGCCGGTGAAAATGAAAGCGAAAGCGCTGAAAAGAGAAGCGGTGGAGGCAGTGAGACCCAATGGAAGTTCATGGAAGGACATGCAAGCCTTTGTCAATATGCTGATCCAACTACCAGTAAGTTAG

mRNA sequence

ATGTCGTCTTCACCGCCGTCGTCCGCCACCTCCCGCCACCTCCTGGTTTTTCCATACCCAGCTCAGGGACATATGATCGCACTCCTCGATCTCACCCATCACTTAGCCTCTCATGGCGGCTTCACAATTACCCTTTTGGTTACCCCCAAAAACCTCCATCTCCTTCAGCCCCTTCTTCAAACCCACCCTTCTGTTCAAACCCTCGTTCTCCCCTTACCTTCTCACCCCAAACTCCCCGTCGGCGTCGAACACGTCGCCCACATCGGCAACCATGGCAATTTCCCGATCATGGCTGCCCTCCGCCAGCTCCACGACCCCATTGTTGAGTGGTTCAACTCTCATCCTTCCCCTCCCGTCGCCATCATTTCCGATTTCTTTCTCGGCTGGACCCAACGTCTCGCCGACCATCTTCGAATTCCCAGAGTTGTTTTCCACTCCATTGCTACATTACTCGTCCATGTAATGAACTCTTGTTGGGTTCAAATTAAAACAGATCATTTTCGTTCTTCCCCTGTCATACAGTTCAGTGAAATCCCCAAATCGCCTTCCTTCAGAAAAGAGCATTTGCCATCGTTGGTAAGGCTGTACCAGGATTCGGACCCCGATTGGAATTTATTGAGGGACGACGCCTTAGGTAATACATCCAGCTGGGCTTGTGTTGTTAATACGTTCGATAGTTTGGAGCACGAGTATTTAGAGCACTTGAGGAAATTGAGGGGCGAAGGGCGTGTATTTGGTATCGGACCGCTTGATATCATCGGCGCTGCAGAGGATGGGCGAAACCCAATTCGAGAGTCGAGTTCAGAGATTCTCACATGGCTGGACAGATGCCCTGATGATTCGGTGCTTTACGTTTGTTTTGGGAGTCAAAAGCAACTGAATCAGCAGCAAATGGAAGCACTGGCTTCCGGGCTTGAGAAAAGCGGTACTCGATTCGTGTGGGTGGTTAAGACATTTCATCAAACAGATGGTGGGTCGGACGGAATACCAATTGGGTTTGAGGATCGGGTGTCAGATCGTGGAATTTTGGCGAAAGGATGGGCGCCGCAAGTGTCAATACTGAACCACCGAGCCGTGGGGGGGTTTCTGAGTCACTGTGGGTGGAACTCGGTGCTTGAGAGCATAGTGGGCGGAGTAATGATACTTGGTTGGCCCATGGAAGCAGACCAGTTTATCAATGCGAGGCTATTGGTAGAAGAGTTGGGAGTAGCGGTGCGCGTTTGCGAGGGGGCGAACTCGGTGCCTGAATCGGACGAGTTGGGGAAGGTCATCGCCGAGTCTTTGAGCGGAGATTCGCCGGTGAAAATGAAAGCGAAAGCGCTGAAAAGAGAAGCGGTGGAGGCAGTGAGACCCAATGGAAGTTCATGGAAGGACATGCAAGCCTTTGTCAATATGCTGATCCAACTACCAGTAAGTTAG

Coding sequence (CDS)

ATGTCGTCTTCACCGCCGTCGTCCGCCACCTCCCGCCACCTCCTGGTTTTTCCATACCCAGCTCAGGGACATATGATCGCACTCCTCGATCTCACCCATCACTTAGCCTCTCATGGCGGCTTCACAATTACCCTTTTGGTTACCCCCAAAAACCTCCATCTCCTTCAGCCCCTTCTTCAAACCCACCCTTCTGTTCAAACCCTCGTTCTCCCCTTACCTTCTCACCCCAAACTCCCCGTCGGCGTCGAACACGTCGCCCACATCGGCAACCATGGCAATTTCCCGATCATGGCTGCCCTCCGCCAGCTCCACGACCCCATTGTTGAGTGGTTCAACTCTCATCCTTCCCCTCCCGTCGCCATCATTTCCGATTTCTTTCTCGGCTGGACCCAACGTCTCGCCGACCATCTTCGAATTCCCAGAGTTGTTTTCCACTCCATTGCTACATTACTCGTCCATGTAATGAACTCTTGTTGGGTTCAAATTAAAACAGATCATTTTCGTTCTTCCCCTGTCATACAGTTCAGTGAAATCCCCAAATCGCCTTCCTTCAGAAAAGAGCATTTGCCATCGTTGGTAAGGCTGTACCAGGATTCGGACCCCGATTGGAATTTATTGAGGGACGACGCCTTAGGTAATACATCCAGCTGGGCTTGTGTTGTTAATACGTTCGATAGTTTGGAGCACGAGTATTTAGAGCACTTGAGGAAATTGAGGGGCGAAGGGCGTGTATTTGGTATCGGACCGCTTGATATCATCGGCGCTGCAGAGGATGGGCGAAACCCAATTCGAGAGTCGAGTTCAGAGATTCTCACATGGCTGGACAGATGCCCTGATGATTCGGTGCTTTACGTTTGTTTTGGGAGTCAAAAGCAACTGAATCAGCAGCAAATGGAAGCACTGGCTTCCGGGCTTGAGAAAAGCGGTACTCGATTCGTGTGGGTGGTTAAGACATTTCATCAAACAGATGGTGGGTCGGACGGAATACCAATTGGGTTTGAGGATCGGGTGTCAGATCGTGGAATTTTGGCGAAAGGATGGGCGCCGCAAGTGTCAATACTGAACCACCGAGCCGTGGGGGGGTTTCTGAGTCACTGTGGGTGGAACTCGGTGCTTGAGAGCATAGTGGGCGGAGTAATGATACTTGGTTGGCCCATGGAAGCAGACCAGTTTATCAATGCGAGGCTATTGGTAGAAGAGTTGGGAGTAGCGGTGCGCGTTTGCGAGGGGGCGAACTCGGTGCCTGAATCGGACGAGTTGGGGAAGGTCATCGCCGAGTCTTTGAGCGGAGATTCGCCGGTGAAAATGAAAGCGAAAGCGCTGAAAAGAGAAGCGGTGGAGGCAGTGAGACCCAATGGAAGTTCATGGAAGGACATGCAAGCCTTTGTCAATATGCTGATCCAACTACCAGTAAGTTAG

Protein sequence

MSSSPPSSATSRHLLVFPYPAQGHMIALLDLTHHLASHGGFTITLLVTPKNLHLLQPLLQTHPSVQTLVLPLPSHPKLPVGVEHVAHIGNHGNFPIMAALRQLHDPIVEWFNSHPSPPVAIISDFFLGWTQRLADHLRIPRVVFHSIATLLVHVMNSCWVQIKTDHFRSSPVIQFSEIPKSPSFRKEHLPSLVRLYQDSDPDWNLLRDDALGNTSSWACVVNTFDSLEHEYLEHLRKLRGEGRVFGIGPLDIIGAAEDGRNPIRESSSEILTWLDRCPDDSVLYVCFGSQKQLNQQQMEALASGLEKSGTRFVWVVKTFHQTDGGSDGIPIGFEDRVSDRGILAKGWAPQVSILNHRAVGGFLSHCGWNSVLESIVGGVMILGWPMEADQFINARLLVEELGVAVRVCEGANSVPESDELGKVIAESLSGDSPVKMKAKALKREAVEAVRPNGSSWKDMQAFVNMLIQLPVS
Homology
BLAST of Lsi04G003350 vs. ExPASy Swiss-Prot
Match: Q9LZD8 (UDP-glycosyltransferase 89A2 OS=Arabidopsis thaliana OX=3702 GN=UGT89A2 PE=2 SV=1)

HSP 1 Score: 461.1 bits (1185), Expect = 1.5e-128
Identity = 242/466 (51.93%), Postives = 319/466 (68.45%), Query Frame = 0

Query: 2   SSSPPSSATSRHLLVFPYPAQGHMIALLDLTHHLASHGGFTITLLVTPKNLHLLQPLLQT 61
           +S PP      H++VFP+PAQGH++ LLDLTH L    GF ++++VTP NL  L PLL  
Sbjct: 14  NSKPP------HIVVFPFPAQGHLLPLLDLTHQLCLR-GFNVSVIVTPGNLTYLSPLLSA 73

Query: 62  HP-SVQTLVLPLPSHPKLPVGVEHVAHIGNHGNFPIMAALRQLHDPIVEWFNSHPSPPVA 121
           HP SV ++V P P HP L  GVE+V  +GN GN PIMA+LRQL +PI+ WF SHP+PP+A
Sbjct: 74  HPSSVTSVVFPFPPHPSLSPGVENVKDVGNSGNLPIMASLRQLREPIINWFQSHPNPPIA 133

Query: 122 IISDFFLGWTQRLADHLRIPRVVFHSIATLLVHVMNSCWVQIKTDHFRSSPVIQFSEIPK 181
           +ISDFFLGWT  L + + IPR  F SI+  LV V+  C+  I  D  +S+  I   ++P+
Sbjct: 134 LISDFFLGWTHDLCNQIGIPRFAFFSISFFLVSVLQFCFENI--DLIKSTDPIHLLDLPR 193

Query: 182 SPSFRKEHLPSLV-RLYQDSDPDWNLLRDDALGNTSSWACVVNTFDSLEHEYLEHLRKLR 241
           +P F++EHLPS+V R  Q   PD   ++D ++ N  S+  V N+ + LE +YL+++++  
Sbjct: 194 APIFKEEHLPSIVRRSLQTPSPDLESIKDFSM-NLLSYGSVFNSSEILEDDYLQYVKQRM 253

Query: 242 GEGRVFGIGPLDIIGAAEDGRNPIRESSSEILTWLDRCPDDSVLYVCFGSQKQLNQQQME 301
           G  RV+ IGPL  IG+     +   + S  +L+WLD  P+ SVLYVCFGSQK L + Q +
Sbjct: 254 GHDRVYVIGPLCSIGSGLKSNSGSVDPS--LLSWLDGSPNGSVLYVCFGSQKALTKDQCD 313

Query: 302 ALASGLEKSGTRFVWVVKTFHQTDGGSDGIPIGFEDRVSDRGILAKGWAPQVSILNHRAV 361
           ALA GLEKS TRFVWVVK         D IP GFEDRVS RG++ +GW  Q+++L H AV
Sbjct: 314 ALALGLEKSMTRFVWVVK--------KDPIPDGFEDRVSGRGLVVRGWVSQLAVLRHVAV 373

Query: 362 GGFLSHCGWNSVLESIVGGVMILGWPMEADQFINARLLVEELGVAVRVCEGANSVPESDE 421
           GGFLSHCGWNSVLE I  G +ILGWPMEADQF+NARLLVE LGVAVRVCEG  +VP+SDE
Sbjct: 374 GGFLSHCGWNSVLEGITSGAVILGWPMEADQFVNARLLVEHLGVAVRVCEGGETVPDSDE 433

Query: 422 LGKVIAESL-SGDSPVKMKAKALKREAVEAV-RPNGSSWKDMQAFV 464
           LG+VIAE++  G   V  +A+ ++R+   AV   NGSS +++Q  V
Sbjct: 434 LGRVIAETMGEGGREVAARAEEIRRKTEAAVTEANGSSVENVQRLV 459

BLAST of Lsi04G003350 vs. ExPASy Swiss-Prot
Match: Q9C9B0 (Flavonol 3-O-glucosyltransferase UGT89B1 OS=Arabidopsis thaliana OX=3702 GN=UGT89B1 PE=1 SV=2)

HSP 1 Score: 446.4 bits (1147), Expect = 3.9e-124
Identity = 220/466 (47.21%), Postives = 307/466 (65.88%), Query Frame = 0

Query: 10  TSRHLLVFPYPAQGHMIALLDLTHHLASHGG--FTITLLVTPKNLHLLQPLLQTHPSVQT 69
           T  H+L+FP+PAQGHMI LLD TH LA  GG    IT+LVTPKNL  L PLL    +++ 
Sbjct: 11  TKTHVLIFPFPAQGHMIPLLDFTHRLALRGGAALKITVLVTPKNLPFLSPLLSAVVNIEP 70

Query: 70  LVLPLPSHPKLPVGVEHVAHIGNHGNFPIMA-ALRQLHDPIVEWFNSHPSPPVAIISDFF 129
           L+LP PSHP +P GVE+V  +   G FP+M  AL  LH P++ W  SHPSPPVAI+SDFF
Sbjct: 71  LILPFPSHPSIPSGVENVQDLPPSG-FPLMIHALGNLHAPLISWITSHPSPPVAIVSDFF 130

Query: 130 LGWTQRLADHLRIPRVVFHSIATLLVHVMNSCWVQI--KTDHFRSSPVIQFSEIPKSPSF 189
           LGWT+ L     IPR  F   A +   ++N+ W+++  K +    + ++ F +IP  P +
Sbjct: 131 LGWTKNLG----IPRFDFSPSAAITCCILNTLWIEMPTKINEDDDNEILHFPKIPNCPKY 190

Query: 190 RKEHLPSLVRLYQDSDPDWNLLRDDALGNTSSWACVVNTFDSLEHEYLEHLRKLRGEGRV 249
           R + + SL R Y   DP W  +RD    N +SW  VVN+F ++E  YLEHL++  G  RV
Sbjct: 191 RFDQISSLYRSYVHGDPAWEFIRDSFRDNVASWGLVVNSFTAMEGVYLEHLKREMGHDRV 250

Query: 250 FGIGPLDIIGAAEDGR-NPIRESSSEILTWLDRCPDDSVLYVCFGSQKQLNQQQMEALAS 309
           + +GP  II  + D R  P   S   +++WLD   D+ V+YVCFGSQ  L ++Q  ALAS
Sbjct: 251 WAVGP--IIPLSGDNRGGPTSVSVDHVMSWLDAREDNHVVYVCFGSQVVLTKEQTLALAS 310

Query: 310 GLEKSGTRFVWVVKTFHQTDGGSDGIPIGFEDRVSDRGILAKGWAPQVSILNHRAVGGFL 369
           GLEKSG  F+W VK   + D     I  GF+DRV+ RG++ +GWAPQV++L HRAVG FL
Sbjct: 311 GLEKSGVHFIWAVKEPVEKDSTRGNILDGFDDRVAGRGLVIRGWAPQVAVLRHRAVGAFL 370

Query: 370 SHCGWNSVLESIVGGVMILGWPMEADQFINARLLVEELGVAVRVCEGANSVPESDELGKV 429
           +HCGWNSV+E++V GV++L WPM ADQ+ +A L+V+EL V VR CEG ++VP+ DEL +V
Sbjct: 371 THCGWNSVVEAVVAGVLMLTWPMRADQYTDASLVVDELKVGVRACEGPDTVPDPDELARV 430

Query: 430 IAESLSGDSPVKMKAKALKREAVEAVRPNGSSWKDMQAFVNMLIQL 470
            A+S++G+   ++KA  L++ A++A++  GSS  D+  F+  ++ L
Sbjct: 431 FADSVTGNQTERIKAVELRKAALDAIQERGSSVNDLDGFIQHVVSL 469

BLAST of Lsi04G003350 vs. ExPASy Swiss-Prot
Match: Q6VAA5 (UDP-glycosyltransferase 89B2 OS=Stevia rebaudiana OX=55670 GN=UGT89B2 PE=2 SV=1)

HSP 1 Score: 414.5 bits (1064), Expect = 1.7e-114
Identity = 218/460 (47.39%), Postives = 291/460 (63.26%), Query Frame = 0

Query: 13  HLLVFPYPAQGHMIALLDLTHHLASHGGFTITLLVTPKNLHLLQPLLQTHP-SVQTLVLP 72
           H+LVFPYPAQGHM+ LLDLTH LA     TIT+LVTPKNL  + PLL  HP +V  L+LP
Sbjct: 11  HILVFPYPAQGHMLTLLDLTHQLAIR-NLTITILVTPKNLPTISPLLAAHPTTVSALLLP 70

Query: 73  LPSHPKLPVGVEHVAHIGNHGNFPIMAALRQLHDPIVEWFNSHPSPPVAIISDFFLGWTQ 132
           LP HP +P G+E+V  + N     +M AL  L++P+ +WF + P+PPVAIISDFFLGWT 
Sbjct: 71  LPPHPAIPSGIENVKDLPNDAFKAMMVALGDLYNPLRDWFRNQPNPPVAIISDFFLGWTH 130

Query: 133 RLADHLRIPRVVFHSIATLLVHVMNSCW--VQIKTDHFRSSPVIQFSEIPKSPSFRKEHL 192
            LA  L I R  F     L + V+ S W     + D       I+F +IP SP +    L
Sbjct: 131 HLAVELGIRRYTFSPSGALALSVIFSLWRYQPKRIDVENEKEAIKFPKIPNSPEYPWWQL 190

Query: 193 PSLVRLYQDSDPDWNLLRDDALGNTSSWACVVNTFDSLEHEYLEHLRKLRGEGRVFGIGP 252
             + R Y + DPD   ++D  L + +SW  V+N+F  LE  Y++HL+   G  +VF +GP
Sbjct: 191 SPIYRSYVEGDPDSEFIKDGFLADIASWGIVINSFTELEQVYVDHLKHELGHDQVFAVGP 250

Query: 253 LDIIGAAEDGRNPIRESSSEILTWLDRCPDDSVLYVCFGSQKQLNQQQMEALASGLEKSG 312
           L   G    GR     SS+++L+WLD C D +V+YVCFGSQ  L   QME +A GLEKS 
Sbjct: 251 LLPPGDKTSGRG--GSSSNDVLSWLDTCADRTVVYVCFGSQMVLTNGQMEVVALGLEKSR 310

Query: 313 TRFVWVVK--TFHQTDGGSDGIPIGFEDRVSDRGILAKGWAPQVSILNHRAVGGFLSHCG 372
            +FVW VK  T          +P GFEDRVS RG++ +GW PQV+IL+H +VG FL+HCG
Sbjct: 311 VKFVWSVKEPTVGHEAANYGRVPPGFEDRVSGRGLVIRGWVPQVAILSHDSVGVFLTHCG 370

Query: 373 WNSVLESIVGGVMILGWPMEADQFINARLLVEELGVAVRVCEGANSVPESDELGKVIAES 432
           WNSV+E++   V++L WPM ADQF NA LL  EL V ++VCEG+N VP SDEL ++ ++S
Sbjct: 371 WNSVMEAVAAEVLMLTWPMSADQFSNATLL-HELKVGIKVCEGSNIVPNSDELAELFSKS 430

Query: 433 LSGDSPVKMK-AKALKREAVEAVRPNGSSWKDMQAFVNML 467
           LS ++ ++ K  K   + A EAV P GSS  +++  V+ L
Sbjct: 431 LSDETRLERKRVKEFAKSAKEAVGPKGSSVGELERLVDNL 466

BLAST of Lsi04G003350 vs. ExPASy Swiss-Prot
Match: Q9LNE6 (Flavonol 7-O-rhamnosyltransferase OS=Arabidopsis thaliana OX=3702 GN=UGT89C1 PE=1 SV=1)

HSP 1 Score: 302.8 bits (774), Expect = 7.0e-81
Identity = 175/464 (37.72%), Postives = 250/464 (53.88%), Query Frame = 0

Query: 13  HLLVFPYPAQGHMIALLDLTHHLASHGGFTITLLVTPKNLHLLQPLLQTH--PSVQTLVL 72
           H+LV P+P  GHM+  LDLTH +   G  T+T+LVTPKN   L  L   H     +TL+L
Sbjct: 10  HVLVIPFPQSGHMVPHLDLTHQILLRGA-TVTVLVTPKNSSYLDALRSLHSPEHFKTLIL 69

Query: 73  PLPSHPKLPVGVEHVAHIGNHGNFPIMAALRQLHDPIVEWFNSHPSP--PVAIISDFFLG 132
           P PSHP +P GVE +  +       +  AL +LHDP+V++ +  P    P AI+   FL 
Sbjct: 70  PFPSHPCIPSGVESLQQLPLEAIVHMFDALSRLHDPLVDFLSRQPPSDLPDAILGSSFLS 129

Query: 133 -WTQRLADHLRIPRVVFHSIATLLVHVMNSCWVQIKTDHFRSSPVIQFSEIPKSPSFRKE 192
            W  ++AD   I  + F  I    + VM   W Q                          
Sbjct: 130 PWINKVADAFSIKSISFLPINAHSISVM---WAQ-------------------------- 189

Query: 193 HLPSLVRLYQDSDPDWNLLRDDALGNTSSWACVVNTFDSLEHEYLEHLR-KLRGEGRVFG 252
                         D +   D     T S+  V+N+F  LE E++E ++ +     R++ 
Sbjct: 190 -------------EDRSFFNDLETATTESYGLVINSFYDLEPEFVETVKTRFLNHHRIWT 249

Query: 253 IGPLDIIGAAEDGRNPIRESSSEILTWLDRCPDD-SVLYVCFGSQKQLNQQQMEALASGL 312
           +GPL    A  D         +++  WLD CP+D SV+YV FGSQ +L  +Q  ALA+ L
Sbjct: 250 VGPLLPFKAGVDRGGQSSIPPAKVSAWLDSCPEDNSVVYVGFGSQIRLTAEQTAALAAAL 309

Query: 313 EKSGTRFVWVVKTFHQTDGGSDG------IPIGFEDRVSDRGILAKGWAPQVSILNHRAV 372
           EKS  RF+W V+   +    SD       IP GFE+RV ++G++ +GWAPQ  IL HRAV
Sbjct: 310 EKSSVRFIWAVRDAAKKVNSSDNSVEEDVIPAGFEERVKEKGLVIRGWAPQTMILEHRAV 369

Query: 373 GGFLSHCGWNSVLESIVGGVMILGWPMEADQFINARLLVEELGVAVRVCEGANSVPESDE 432
           G +L+H GW SVLE +VGGVM+L WPM+AD F N  L+V++L  AVRV E  +SVP+SD+
Sbjct: 370 GSYLTHLGWGSVLEGMVGGVMLLAWPMQADHFFNTTLIVDKLRAAVRVGENRDSVPDSDK 429

Query: 433 LGKVIAESLSGDSPVKMKAKALKREAVEAVRPNGSSWKDMQAFV 464
           L +++AES   D P ++    L+ +A+EA++  GSS+K++   V
Sbjct: 430 LARILAESAREDLPERVTLMKLREKAMEAIKEGGSSYKNLDELV 430

BLAST of Lsi04G003350 vs. ExPASy Swiss-Prot
Match: K4GMD6 (UDP-glycosyltransferase 73C9 OS=Barbarea vulgaris OX=50459 GN=UGT73C9 PE=3 SV=1)

HSP 1 Score: 241.5 bits (615), Expect = 1.9e-62
Identity = 166/494 (33.60%), Postives = 260/494 (52.63%), Query Frame = 0

Query: 13  HLLVFPYPAQGHMIALLDLTHHLASHGGFTITLLVTPKNL----HLLQPLLQTHPSVQTL 72
           H ++FPY AQGHMI ++D+   LA   G  IT++ TP+N     ++L   +++   +  +
Sbjct: 13  HFVLFPYMAQGHMIPMVDIARLLAQR-GVKITIVTTPQNAARFENVLSRAIESGLPISIV 72

Query: 73  VLPLPSHPK-LPVGVE-HVAHIGNHGNFPIMAALRQLHDPIVEWFNSHPSPPVAIISDFF 132
            + LPS    LP G+E   + +      P   A+  L +P+ + F      P  IISDF 
Sbjct: 73  QVKLPSQEAGLPEGIETFESLVSMELLVPFFKAVNMLEEPVQKLFEEMSPQPSCIISDFC 132

Query: 133 LGWTQRLADHLRIPRVVFHSI---ATLLVHVM-NSCWV--QIKTD--HFRSSPVIQFSEI 192
           L +T ++A    IP+++FH +     L +HV+  +C +   +K+D  HF    V+ +   
Sbjct: 133 LHYTSKIAKKFNIPKILFHGMCCFCLLCMHVLRKNCEILENLKSDKEHF----VVPY--F 192

Query: 193 PKSPSFRKEHLPSLVRLYQDSDPDWNLLRDDAL-GNTSSWACVVNTFDSLEHEYLEHLRK 252
           P    F +  +P    +   +  DW  +R+D +  + +S+  +VNT+  LE  Y    ++
Sbjct: 193 PDRVEFTRPQVP----MATYAPGDWQEIREDIVEADKTSYGVIVNTYQELEPAYANDYKE 252

Query: 253 LRGEGRVFGIGPLDI---IGA--AEDGRNPIRESSSEILTWLDRCPDDSVLYVCFGSQKQ 312
            R  G+ + IGP+ +   +GA  AE G N       E L WLD   + SVLYVC GS   
Sbjct: 253 AR-SGKAWTIGPVSLCNKVGADKAERG-NKADIDQDECLKWLDSKEEGSVLYVCLGSNCS 312

Query: 313 LNQQQMEALASGLEKSGTRFVWVVKTFHQTDGGSDGI-PIGFEDRVSDRGILAKGWAPQV 372
           +   Q++ L  GLE+S   F+WVV+ + +     +     GFE+RV DRG+L KGW+PQ+
Sbjct: 313 VPLSQLKELGLGLEESQRPFIWVVRGWEKNKELLEWFSESGFEERVKDRGLLIKGWSPQM 372

Query: 373 SILNHRAVGGFLSHCGWNSVLESIVGGVMILGWPMEADQFINARLLVEELGVAVRV-CEG 432
            IL H +VGGFL+HCGWNS LE I  G+ +L WP+  DQF N +L+V+ L V V    E 
Sbjct: 373 LILAHHSVGGFLTHCGWNSTLEGITSGIPLLTWPLIVDQFCNQKLVVQVLKVGVSAGVEE 432

Query: 433 ANSVPESDELG--------KVIAESLSGDS----PVKMKAKALKREAVEAVRPNGSSWKD 473
             +  E +++G        K   E L G+S      + + KAL + A +AV   GSS  +
Sbjct: 433 VTNWGEEEKIGVLVDKEGVKKAVEELMGESDDAKERRKRVKALGQLAHKAVEEGGSSHSN 492

BLAST of Lsi04G003350 vs. ExPASy TrEMBL
Match: A0A0A0LCK3 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G640640 PE=4 SV=1)

HSP 1 Score: 837.4 bits (2162), Expect = 2.9e-239
Identity = 394/470 (83.83%), Postives = 439/470 (93.40%), Query Frame = 0

Query: 1   MSSSPPSSATSRHLLVFPYPAQGHMIALLDLTHHLASHGGFTITLLVTPKNLHLLQPLLQ 60
           MSSS P SAT+RHLLVFPYPAQGHM+ LLDLT+HLASHGGFTIT+LVTPK L LL PLLQ
Sbjct: 43  MSSSLPPSATTRHLLVFPYPAQGHMLPLLDLTNHLASHGGFTITILVTPKTLPLLHPLLQ 102

Query: 61  THPSVQTLVLPLPSHPKLPVGVEHVAHIGNHGNFPIMAALRQLHDPIVEWFNSHPSPPVA 120
           THPS+QTLVLP PSHPKLPVGVEHV+HIGNHGNF I+AALRQLHDPIV+WFNSHPSPPVA
Sbjct: 103 THPSIQTLVLPFPSHPKLPVGVEHVSHIGNHGNFAIVAALRQLHDPIVDWFNSHPSPPVA 162

Query: 121 IISDFFLGWTQRLADHLRIPRVVFHSIATLLVHVMNSCWVQIKTDHFRSSPVIQFSEIPK 180
           IISDFFLGWTQRLADHL+IPRV F+++++LL+HVMNSCWV IKTDHF SSPVI+FSEIPK
Sbjct: 163 IISDFFLGWTQRLADHLQIPRVAFYAVSSLLIHVMNSCWVHIKTDHFSSSPVIEFSEIPK 222

Query: 181 SPSFRKEHLPSLVRLYQDSDPDWNLLRDDALGNTSSWACVVNTFDSLEHEYLEHLRKLRG 240
           SPSF+KE LPSLV+ YQDSDPDWNLLRDD L NTSSWACVV+TF++L+ EYL+HLRKL G
Sbjct: 223 SPSFKKEQLPSLVKQYQDSDPDWNLLRDDVLANTSSWACVVDTFENLDLEYLDHLRKLWG 282

Query: 241 EGRVFGIGPLDIIGAAEDGRNPIRESSSEILTWLDRCPDDSVLYVCFGSQKQLNQQQMEA 300
           EGRVFG+GP+ +IGA +DGRNPIRESSSEILTWLD+CPDDSV+YVCFGSQKQL++QQ+EA
Sbjct: 283 EGRVFGVGPVHLIGATKDGRNPIRESSSEILTWLDKCPDDSVVYVCFGSQKQLSRQQLEA 342

Query: 301 LASGLEKSGTRFVWVVKTFHQTDGGSDGIPIGFEDRVSDRGILAKGWAPQVSILNHRAVG 360
           LAS LEKSGTRFVWVVKT HQTDG S+GIP+GFEDRVSDRGI+ KGW PQ +IL+HRAVG
Sbjct: 343 LASALEKSGTRFVWVVKTIHQTDGRSNGIPVGFEDRVSDRGIVVKGWVPQTAILHHRAVG 402

Query: 361 GFLSHCGWNSVLESIVGGVMILGWPMEADQFINARLLVEELGVAVRVCEGANSVPESDEL 420
           GFLSHCGWNSV+ESI  GVM+LGWPMEADQFINARLLVE+LGVAVRVCEGANSVPES+EL
Sbjct: 403 GFLSHCGWNSVVESIANGVMVLGWPMEADQFINARLLVEDLGVAVRVCEGANSVPESEEL 462

Query: 421 GKVIAESLSGDSPVKMKAKALKREAVEAVRPNGSSWKDMQAFVNMLIQLP 471
           GK+IAESLS DS  KMKAKALKR+AVEAVRPNGSSWKDMQAF++ LIQLP
Sbjct: 463 GKIIAESLSRDSSEKMKAKALKRKAVEAVRPNGSSWKDMQAFIDKLIQLP 512

BLAST of Lsi04G003350 vs. ExPASy TrEMBL
Match: A0A1S3CC75 (UDP-glycosyltransferase 89A2-like OS=Cucumis melo OX=3656 GN=LOC103499149 PE=4 SV=1)

HSP 1 Score: 825.9 bits (2132), Expect = 8.8e-236
Identity = 394/470 (83.83%), Postives = 434/470 (92.34%), Query Frame = 0

Query: 1   MSSSPPSSATSRHLLVFPYPAQGHMIALLDLTHHLASHGGFTITLLVTPKNLHLLQPLLQ 60
           MSSSPP SAT+RH+LVFPYPAQGHM+ LLDLT+HLASHGGFTIT+LVTPK L LL PLLQ
Sbjct: 1   MSSSPPPSATNRHVLVFPYPAQGHMLPLLDLTNHLASHGGFTITILVTPKILPLLHPLLQ 60

Query: 61  THPSVQTLVLPLPSHPKLPVGVEHVAHIGNHGNFPIMAALRQLHDPIVEWFNSHPSPPVA 120
           THPS+QTLVLP PSHPKLPVG EHV+HIGNHGNF IM ALRQLHDPIVEWF+SHPSPPVA
Sbjct: 61  THPSIQTLVLPFPSHPKLPVGAEHVSHIGNHGNFLIMTALRQLHDPIVEWFSSHPSPPVA 120

Query: 121 IISDFFLGWTQRLADHLRIPRVVFHSIATLLVHVMNSCWVQIKTDHFRSSPVIQFSEIPK 180
           IISDFFLGWTQ LADHL+IPRV F+S+++LLVHVMNSCWV IKTD FRSSPVI+F+EIPK
Sbjct: 121 IISDFFLGWTQCLADHLQIPRVAFYSVSSLLVHVMNSCWVHIKTDCFRSSPVIEFTEIPK 180

Query: 181 SPSFRKEHLPSLVRLYQDSDPDWNLLRDDALGNTSSWACVVNTFDSLEHEYLEHLRKLRG 240
           SPSF+KE LPSLV+ YQDSDPDWNLLRDD L NT SWACVV+TF++L+ EYL+HLRKL G
Sbjct: 181 SPSFKKEQLPSLVKQYQDSDPDWNLLRDDVLANTCSWACVVDTFENLDLEYLDHLRKLWG 240

Query: 241 EGRVFGIGPLDIIGAAEDGRNPIRESSSEILTWLDRCPDDSVLYVCFGSQKQLNQQQMEA 300
           EGRVFG+GPL +IGAA+DGRNPIRESSSEILTWLD+CPDDSV+YVCFGSQKQL++QQ+EA
Sbjct: 241 EGRVFGVGPLHLIGAAKDGRNPIRESSSEILTWLDKCPDDSVVYVCFGSQKQLSRQQVEA 300

Query: 301 LASGLEKSGTRFVWVVKTFHQTDGGSDGIPIGFEDRVSDRGILAKGWAPQVSILNHRAVG 360
           LASGLEKSG RFVWVVKT H+TDG S+GIP+GFEDRVS+RGIL KGW  QV+ILNHRAVG
Sbjct: 301 LASGLEKSGARFVWVVKTIHETDGRSNGIPVGFEDRVSNRGILVKGWVSQVAILNHRAVG 360

Query: 361 GFLSHCGWNSVLESIVGGVMILGWPMEADQFINARLLVEELGVAVRVCEGANSVPESDEL 420
           GFLSHCGWNSVLESI  GVM+LGWPMEADQFINARLLVE+ GVAVR+CEGANSVPES+EL
Sbjct: 361 GFLSHCGWNSVLESIGSGVMVLGWPMEADQFINARLLVEDWGVAVRICEGANSVPESEEL 420

Query: 421 GKVIAESLSGDSPVKMKAKALKREAVEAVRPNGSSWKDMQAFVNMLIQLP 471
           GKVIAESLS DS  KMKAKALKREAV AVRPNGSSWKDMQAF+  LIQLP
Sbjct: 421 GKVIAESLSRDSSEKMKAKALKREAVGAVRPNGSSWKDMQAFIYKLIQLP 470

BLAST of Lsi04G003350 vs. ExPASy TrEMBL
Match: A0A6J1HY91 (UDP-glycosyltransferase 89A2-like OS=Cucurbita maxima OX=3661 GN=LOC111467348 PE=4 SV=1)

HSP 1 Score: 753.4 bits (1944), Expect = 5.5e-214
Identity = 360/470 (76.60%), Postives = 410/470 (87.23%), Query Frame = 0

Query: 1   MSSSPPSSATSRHLLVFPYPAQGHMIALLDLTHHLASHGGFTITLLVTPKNLHLLQPLLQ 60
           MSSSPPS+ T+ HLLVFPYPAQGHMI+LLDLTH+LASHGGFTIT+LVTPKNL LLQPLLQ
Sbjct: 1   MSSSPPST-TTPHLLVFPYPAQGHMISLLDLTHYLASHGGFTITVLVTPKNLPLLQPLLQ 60

Query: 61  THPSVQTLVLPLPSHPKLPVGVEHVAHIGNHGNFPIMAALRQLHDPIVEWFNSHPSPPVA 120
            HPS+ TLVLP P+HP LP G EHV+ IGNHGNFPIM ALRQLHDPIV WFNSHP+PP A
Sbjct: 61  AHPSIHTLVLPFPAHPNLPAGAEHVSQIGNHGNFPIMVALRQLHDPIVHWFNSHPAPPAA 120

Query: 121 IISDFFLGWTQRLADHLRIPRVVFHSIATLLVHVMNSCWVQIKTDHFRSSPVIQFSEIPK 180
           IISDFFLGWTQ LADHLRIPR+V++S    LV+V++  W+ IKTD FRSSPV  F+EIP 
Sbjct: 121 IISDFFLGWTQHLADHLRIPRIVYYSCGAFLVNVLHYSWLHIKTDLFRSSPVFHFNEIPN 180

Query: 181 SPSFRKEHLPSLVRLYQDSDPDWNLLRDDALGNTSSWACVVNTFDSLEHEYLEHLRKLRG 240
           SPSF+K+HLPSL++ YQDS+ DW+LLR+DAL NTS W  V N+F++LE E+LEH R L G
Sbjct: 181 SPSFKKDHLPSLIKRYQDSNLDWDLLRNDALANTSGWVSVFNSFETLEGEFLEHFRDLNG 240

Query: 241 EGRVFGIGPLDIIGAAEDGRNPIRESSSEILTWLDRCPDDSVLYVCFGSQKQLNQQQMEA 300
           EGRVFGIGPL++IGA  DGR+ IR+SSSE+LTWLD C D SVLYVCFGSQKQLNQQQM+A
Sbjct: 241 EGRVFGIGPLNLIGAG-DGRSSIRDSSSEVLTWLDGCADSSVLYVCFGSQKQLNQQQMKA 300

Query: 301 LASGLEKSGTRFVWVVKTFHQTDGGSDGIPIGFEDRVSDRGILAKGWAPQVSILNHRAVG 360
           LASGLEKSG RFVWVVKTF+QT GG + IPIGFEDRVSDRGI+ K W PQV+ILNH+AVG
Sbjct: 301 LASGLEKSGARFVWVVKTFNQTVGGCNEIPIGFEDRVSDRGIVVKEWVPQVAILNHQAVG 360

Query: 361 GFLSHCGWNSVLESIVGGVMILGWPMEADQFINARLLVEELGVAVRVCEGANSVPESDEL 420
           GFLSHCGWNSVLES+  GV+ILGWPMEADQF+NA+LLV+++GVAVRVCEGANSVP+SDEL
Sbjct: 361 GFLSHCGWNSVLESVTAGVLILGWPMEADQFVNAKLLVDDMGVAVRVCEGANSVPDSDEL 420

Query: 421 GKVIAESLSGDSPVKMKAKALKREAVEAVRPNGSSWKDMQAFVNMLIQLP 471
           GKVIA SLSGD P KMKA ALKR AV+AVR NGSSWKDM+ FV+ L QLP
Sbjct: 421 GKVIAMSLSGDRPEKMKAGALKRAAVDAVRSNGSSWKDMRGFVDKLNQLP 468

BLAST of Lsi04G003350 vs. ExPASy TrEMBL
Match: A0A6J1FZE5 (UDP-glycosyltransferase 89A2-like OS=Cucurbita moschata OX=3662 GN=LOC111449303 PE=4 SV=1)

HSP 1 Score: 751.5 bits (1939), Expect = 2.1e-213
Identity = 359/473 (75.90%), Postives = 408/473 (86.26%), Query Frame = 0

Query: 1   MSSS---PPSSATSRHLLVFPYPAQGHMIALLDLTHHLASHGGFTITLLVTPKNLHLLQP 60
           MSSS   PP S T+ HLLVFPYPAQGHMI+LLDLTH+LASHGGFTIT+LVTPKNL LL+P
Sbjct: 1   MSSSPPLPPPSTTTPHLLVFPYPAQGHMISLLDLTHYLASHGGFTITVLVTPKNLPLLEP 60

Query: 61  LLQTHPSVQTLVLPLPSHPKLPVGVEHVAHIGNHGNFPIMAALRQLHDPIVEWFNSHPSP 120
           LLQ HPS+ TLVLP P+HP LP G EHV+ IGNHGNFPIM ALRQLHDPIV WFNSHP+P
Sbjct: 61  LLQAHPSIHTLVLPFPAHPNLPAGAEHVSQIGNHGNFPIMVALRQLHDPIVHWFNSHPAP 120

Query: 121 PVAIISDFFLGWTQRLADHLRIPRVVFHSIATLLVHVMNSCWVQIKTDHFRSSPVIQFSE 180
           P AIISD FLGWTQ LADHL+IPR+V++S    LV+V++  W+ IKTD FRSSPV  F+E
Sbjct: 121 PAAIISDSFLGWTQHLADHLQIPRIVYYSCGAFLVNVLHYSWLHIKTDLFRSSPVFHFNE 180

Query: 181 IPKSPSFRKEHLPSLVRLYQDSDPDWNLLRDDALGNTSSWACVVNTFDSLEHEYLEHLRK 240
           IP SPSF+K+HLPSL++ YQDS+PDW+LLRDDAL NTS W  V N+F++LE E+LEH R 
Sbjct: 181 IPNSPSFKKDHLPSLIKRYQDSNPDWDLLRDDALANTSGWVSVFNSFETLEGEFLEHFRD 240

Query: 241 LRGEGRVFGIGPLDIIGAAEDGRNPIRESSSEILTWLDRCPDDSVLYVCFGSQKQLNQQQ 300
           L GEGRVFGIGPL++I A  DGR+ IR+SSSE+LTWLD C D SVLYVCFGSQKQLNQQQ
Sbjct: 241 LNGEGRVFGIGPLNLIRAG-DGRSSIRDSSSEVLTWLDGCADSSVLYVCFGSQKQLNQQQ 300

Query: 301 MEALASGLEKSGTRFVWVVKTFHQTDGGSDGIPIGFEDRVSDRGILAKGWAPQVSILNHR 360
           MEALASGLE+SG RFVWVVKTF+QT GG +GIPIGFEDRVSDRGI+ K W PQV+ILNHR
Sbjct: 301 MEALASGLERSGARFVWVVKTFNQTVGGCNGIPIGFEDRVSDRGIVVKEWVPQVAILNHR 360

Query: 361 AVGGFLSHCGWNSVLESIVGGVMILGWPMEADQFINARLLVEELGVAVRVCEGANSVPES 420
           AVGGFLSHCGWNSVLES+  GV+ILGWPMEADQF+NA+LLV+++GVAVRVCEGANSVP+S
Sbjct: 361 AVGGFLSHCGWNSVLESVTAGVLILGWPMEADQFVNAKLLVDDMGVAVRVCEGANSVPDS 420

Query: 421 DELGKVIAESLSGDSPVKMKAKALKREAVEAVRPNGSSWKDMQAFVNMLIQLP 471
           DELGKVIA SL GD P KMKA ALKR AV+AVR NGSSWKDM+ FV+ L QLP
Sbjct: 421 DELGKVIAMSLRGDCPEKMKAGALKRAAVDAVRSNGSSWKDMRGFVDKLKQLP 472

BLAST of Lsi04G003350 vs. ExPASy TrEMBL
Match: A0A346A6C1 (Flavonoids UDP-glycosyltransferase OS=Siraitia grosvenorii OX=190515 GN=UGT89M1 PE=2 SV=1)

HSP 1 Score: 737.3 bits (1902), Expect = 4.1e-209
Identity = 364/475 (76.63%), Postives = 406/475 (85.47%), Query Frame = 0

Query: 1   MSSS---PPSSATSRHLLVFPYPAQGHMIALLDLTHHLASHGGFTITLLVTPKNLHLLQP 60
           MSSS   PP S +SRHLLVFPYPAQGHM+ LLDLT  LAS+ GFTIT+LVTPKNL LLQP
Sbjct: 1   MSSSSPPPPPSTSSRHLLVFPYPAQGHMLPLLDLTRRLASY-GFTITVLVTPKNLSLLQP 60

Query: 61  LLQTHPSVQTLVLPLPSHPKLPVGVEHVAHIGNHGNFPIMAALRQLHDPIVEWFNSHPSP 120
           LL THPSV TLVLP P HPKLP GVEHV  IGN GNFPIM ALR+LH PI+ WF+SHPSP
Sbjct: 61  LLSTHPSVHTLVLPFPPHPKLPPGVEHVRDIGNLGNFPIMLALRELHGPILHWFDSHPSP 120

Query: 121 PVAIISDFFLGWTQRLADHLRIPRVVFHSIATLLVHVMNSCWVQIKTDHFRSSPVIQFSE 180
           PVAIISDFFLGWTQRLAD L IPR+VF+S     V+V++ CWV IKTD+FRSSPVI+   
Sbjct: 121 PVAIISDFFLGWTQRLADDLGIPRIVFYSSGAFFVNVVDYCWVHIKTDYFRSSPVIEICS 180

Query: 181 IPKSPSFRKEHLPSLVRLYQDSDPDWNLLRDDALGNTSSWACVVNTFDSLEHEYLEHLRK 240
           IPKSPSF+KEHLPS+VR Y+DSDPD +LLRD A+ N SSWA + NTF+ LEHE+LEHLRK
Sbjct: 181 IPKSPSFKKEHLPSMVRAYKDSDPDSDLLRDSAMINKSSWATIFNTFEKLEHEHLEHLRK 240

Query: 241 LRGEGRVFGIGPLDIIGAAEDGRNPIRESSSEILTWLDRCPDDSVLYVCFGSQKQLNQQQ 300
           +RGEG VFG+GPL +IG AEDGRNPIR+ SS++L WLD CPD SVLYVCFGSQKQLNQQQ
Sbjct: 241 VRGEGSVFGVGPLCLIG-AEDGRNPIRDLSSDVLKWLDGCPDGSVLYVCFGSQKQLNQQQ 300

Query: 301 MEALASGLEKSGTRFVWVVKTFHQTDGGSDGIPIGFEDRVSDRGILAKGWAPQVSILNHR 360
            EALASGLEKSG RFVWVVKT  QT+GGSDGIPIGFEDRVSDRG + K WAPQV ILNH+
Sbjct: 301 TEALASGLEKSGARFVWVVKTI-QTEGGSDGIPIGFEDRVSDRGFVVKEWAPQVLILNHQ 360

Query: 361 AVGGFLSHCGWNSVLESIVGGVMILGWPMEADQFINARLLVEELGVAVRVCEGANSVPES 420
           AVGGFLSHCGWNSVLESIV GVMILGWPMEADQFINARLLV+++GV+VRVCEGANSVP+ 
Sbjct: 361 AVGGFLSHCGWNSVLESIVAGVMILGWPMEADQFINARLLVDDMGVSVRVCEGANSVPDP 420

Query: 421 DELGKVIAESLSGDSPVKMKAKALKREAVEAVRPNGSSWKDMQAFVNMLIQLPVS 473
           DELGKVIA+SL G+SP KMKAKAL+REAV+A+  NGSSW DM   VN L +LP S
Sbjct: 421 DELGKVIAKSLRGESPEKMKAKALRREAVDAMGTNGSSWNDMHDLVNKLNELPPS 472

BLAST of Lsi04G003350 vs. NCBI nr
Match: XP_038893299.1 (UDP-glycosyltransferase 89A2-like [Benincasa hispida])

HSP 1 Score: 883.6 bits (2282), Expect = 7.3e-253
Identity = 424/472 (89.83%), Postives = 451/472 (95.55%), Query Frame = 0

Query: 1   MSSSPPSSATSRHLLVFPYPAQGHMIALLDLTHHLASHGGFTITLLVTPKNLHLLQPLLQ 60
           MSS+PP SATSRHLLV PYPAQGHM+ALLDLT+HLASHGGFTITLLVTPKNL LLQPLLQ
Sbjct: 1   MSSAPPPSATSRHLLVIPYPAQGHMLALLDLTNHLASHGGFTITLLVTPKNLPLLQPLLQ 60

Query: 61  THPSVQTLVLPLPSHPKLPVGVEHVAHIGNHGNFPIMAALRQLHDPIVEWFNSHPSPPVA 120
           THPS+QTLVLPLPSHPKLPVGVEHV+HIGNHGNFPIMAALRQLHD IVEWFNSHPSPPVA
Sbjct: 61  THPSLQTLVLPLPSHPKLPVGVEHVSHIGNHGNFPIMAALRQLHDSIVEWFNSHPSPPVA 120

Query: 121 IISDFFLGWTQRLADHLRIPRVVFHSIATLLVHVMNSCWVQIKTDHFRSSPVIQFSEIPK 180
           IISD FLGWTQRLADHL+IPRVVFHS++T L+HVMNSCWV IKTD FRSSPVIQFSEIPK
Sbjct: 121 IISDSFLGWTQRLADHLQIPRVVFHSVSTFLIHVMNSCWVHIKTDRFRSSPVIQFSEIPK 180

Query: 181 SPSFRKEHLPSLVRLYQDSDPDWNLLRDDALGNTSSWACVVNTFDSLEHEYLEHLRKLRG 240
           SPS +KEHLPSLV+LY DSDPDWNLLRDDAL NTSSWACVVNTFD+LEH+YLEHLRKL G
Sbjct: 181 SPSLKKEHLPSLVKLYLDSDPDWNLLRDDALDNTSSWACVVNTFDNLEHDYLEHLRKLGG 240

Query: 241 EGRVFGIGPLDIIGAAEDGRNPIRESSSEILTWLDRCPDDSVLYVCFGSQKQLNQQQMEA 300
           EGRVFG+GPLDIIGAAEDGRNPIRESSSEILTWLD+CPD SV+YVCFGSQKQLNQQQMEA
Sbjct: 241 EGRVFGVGPLDIIGAAEDGRNPIRESSSEILTWLDKCPDGSVVYVCFGSQKQLNQQQMEA 300

Query: 301 LASGLEKSGTRFVWVVKTFHQTDGGSDGIPIGFEDRVSDRGILAKGWAPQVSILNHRAVG 360
           LASGLEKSGTRFVWVVKT H+TD GS+GIPIGFEDRVSDRGIL KGWAPQV+ILNHRAVG
Sbjct: 301 LASGLEKSGTRFVWVVKTIHRTDSGSNGIPIGFEDRVSDRGILVKGWAPQVAILNHRAVG 360

Query: 361 GFLSHCGWNSVLESIVGGVMILGWPMEADQFINARLLVEELGVAVRVCEGANSVPESDEL 420
           GFLSHCGWNSVLESIV GVMI+GWPMEADQFINARLLVEELGVAVRVCEGANSVPESD+L
Sbjct: 361 GFLSHCGWNSVLESIVRGVMIVGWPMEADQFINARLLVEELGVAVRVCEGANSVPESDKL 420

Query: 421 GKVIAESLSGDSPVKMKAKALKREAVEAVRPNGSSWKDMQAFVNMLIQLPVS 473
           GKVIAES+SGDSP K+KAKAL+REAVEAVR NGSSWKDMQ FVN L+QLP++
Sbjct: 421 GKVIAESMSGDSPEKIKAKALRREAVEAVRSNGSSWKDMQDFVNKLMQLPLN 472

BLAST of Lsi04G003350 vs. NCBI nr
Match: XP_031739347.1 (UDP-glycosyltransferase 89A2 [Cucumis sativus] >KAE8650874.1 hypothetical protein Csa_000845 [Cucumis sativus])

HSP 1 Score: 837.4 bits (2162), Expect = 6.0e-239
Identity = 394/470 (83.83%), Postives = 439/470 (93.40%), Query Frame = 0

Query: 1   MSSSPPSSATSRHLLVFPYPAQGHMIALLDLTHHLASHGGFTITLLVTPKNLHLLQPLLQ 60
           MSSS P SAT+RHLLVFPYPAQGHM+ LLDLT+HLASHGGFTIT+LVTPK L LL PLLQ
Sbjct: 1   MSSSLPPSATTRHLLVFPYPAQGHMLPLLDLTNHLASHGGFTITILVTPKTLPLLHPLLQ 60

Query: 61  THPSVQTLVLPLPSHPKLPVGVEHVAHIGNHGNFPIMAALRQLHDPIVEWFNSHPSPPVA 120
           THPS+QTLVLP PSHPKLPVGVEHV+HIGNHGNF I+AALRQLHDPIV+WFNSHPSPPVA
Sbjct: 61  THPSIQTLVLPFPSHPKLPVGVEHVSHIGNHGNFAIVAALRQLHDPIVDWFNSHPSPPVA 120

Query: 121 IISDFFLGWTQRLADHLRIPRVVFHSIATLLVHVMNSCWVQIKTDHFRSSPVIQFSEIPK 180
           IISDFFLGWTQRLADHL+IPRV F+++++LL+HVMNSCWV IKTDHF SSPVI+FSEIPK
Sbjct: 121 IISDFFLGWTQRLADHLQIPRVAFYAVSSLLIHVMNSCWVHIKTDHFSSSPVIEFSEIPK 180

Query: 181 SPSFRKEHLPSLVRLYQDSDPDWNLLRDDALGNTSSWACVVNTFDSLEHEYLEHLRKLRG 240
           SPSF+KE LPSLV+ YQDSDPDWNLLRDD L NTSSWACVV+TF++L+ EYL+HLRKL G
Sbjct: 181 SPSFKKEQLPSLVKQYQDSDPDWNLLRDDVLANTSSWACVVDTFENLDLEYLDHLRKLWG 240

Query: 241 EGRVFGIGPLDIIGAAEDGRNPIRESSSEILTWLDRCPDDSVLYVCFGSQKQLNQQQMEA 300
           EGRVFG+GP+ +IGA +DGRNPIRESSSEILTWLD+CPDDSV+YVCFGSQKQL++QQ+EA
Sbjct: 241 EGRVFGVGPVHLIGATKDGRNPIRESSSEILTWLDKCPDDSVVYVCFGSQKQLSRQQLEA 300

Query: 301 LASGLEKSGTRFVWVVKTFHQTDGGSDGIPIGFEDRVSDRGILAKGWAPQVSILNHRAVG 360
           LAS LEKSGTRFVWVVKT HQTDG S+GIP+GFEDRVSDRGI+ KGW PQ +IL+HRAVG
Sbjct: 301 LASALEKSGTRFVWVVKTIHQTDGRSNGIPVGFEDRVSDRGIVVKGWVPQTAILHHRAVG 360

Query: 361 GFLSHCGWNSVLESIVGGVMILGWPMEADQFINARLLVEELGVAVRVCEGANSVPESDEL 420
           GFLSHCGWNSV+ESI  GVM+LGWPMEADQFINARLLVE+LGVAVRVCEGANSVPES+EL
Sbjct: 361 GFLSHCGWNSVVESIANGVMVLGWPMEADQFINARLLVEDLGVAVRVCEGANSVPESEEL 420

Query: 421 GKVIAESLSGDSPVKMKAKALKREAVEAVRPNGSSWKDMQAFVNMLIQLP 471
           GK+IAESLS DS  KMKAKALKR+AVEAVRPNGSSWKDMQAF++ LIQLP
Sbjct: 421 GKIIAESLSRDSSEKMKAKALKRKAVEAVRPNGSSWKDMQAFIDKLIQLP 470

BLAST of Lsi04G003350 vs. NCBI nr
Match: XP_008460270.1 (PREDICTED: UDP-glycosyltransferase 89A2-like [Cucumis melo])

HSP 1 Score: 825.9 bits (2132), Expect = 1.8e-235
Identity = 394/470 (83.83%), Postives = 434/470 (92.34%), Query Frame = 0

Query: 1   MSSSPPSSATSRHLLVFPYPAQGHMIALLDLTHHLASHGGFTITLLVTPKNLHLLQPLLQ 60
           MSSSPP SAT+RH+LVFPYPAQGHM+ LLDLT+HLASHGGFTIT+LVTPK L LL PLLQ
Sbjct: 1   MSSSPPPSATNRHVLVFPYPAQGHMLPLLDLTNHLASHGGFTITILVTPKILPLLHPLLQ 60

Query: 61  THPSVQTLVLPLPSHPKLPVGVEHVAHIGNHGNFPIMAALRQLHDPIVEWFNSHPSPPVA 120
           THPS+QTLVLP PSHPKLPVG EHV+HIGNHGNF IM ALRQLHDPIVEWF+SHPSPPVA
Sbjct: 61  THPSIQTLVLPFPSHPKLPVGAEHVSHIGNHGNFLIMTALRQLHDPIVEWFSSHPSPPVA 120

Query: 121 IISDFFLGWTQRLADHLRIPRVVFHSIATLLVHVMNSCWVQIKTDHFRSSPVIQFSEIPK 180
           IISDFFLGWTQ LADHL+IPRV F+S+++LLVHVMNSCWV IKTD FRSSPVI+F+EIPK
Sbjct: 121 IISDFFLGWTQCLADHLQIPRVAFYSVSSLLVHVMNSCWVHIKTDCFRSSPVIEFTEIPK 180

Query: 181 SPSFRKEHLPSLVRLYQDSDPDWNLLRDDALGNTSSWACVVNTFDSLEHEYLEHLRKLRG 240
           SPSF+KE LPSLV+ YQDSDPDWNLLRDD L NT SWACVV+TF++L+ EYL+HLRKL G
Sbjct: 181 SPSFKKEQLPSLVKQYQDSDPDWNLLRDDVLANTCSWACVVDTFENLDLEYLDHLRKLWG 240

Query: 241 EGRVFGIGPLDIIGAAEDGRNPIRESSSEILTWLDRCPDDSVLYVCFGSQKQLNQQQMEA 300
           EGRVFG+GPL +IGAA+DGRNPIRESSSEILTWLD+CPDDSV+YVCFGSQKQL++QQ+EA
Sbjct: 241 EGRVFGVGPLHLIGAAKDGRNPIRESSSEILTWLDKCPDDSVVYVCFGSQKQLSRQQVEA 300

Query: 301 LASGLEKSGTRFVWVVKTFHQTDGGSDGIPIGFEDRVSDRGILAKGWAPQVSILNHRAVG 360
           LASGLEKSG RFVWVVKT H+TDG S+GIP+GFEDRVS+RGIL KGW  QV+ILNHRAVG
Sbjct: 301 LASGLEKSGARFVWVVKTIHETDGRSNGIPVGFEDRVSNRGILVKGWVSQVAILNHRAVG 360

Query: 361 GFLSHCGWNSVLESIVGGVMILGWPMEADQFINARLLVEELGVAVRVCEGANSVPESDEL 420
           GFLSHCGWNSVLESI  GVM+LGWPMEADQFINARLLVE+ GVAVR+CEGANSVPES+EL
Sbjct: 361 GFLSHCGWNSVLESIGSGVMVLGWPMEADQFINARLLVEDWGVAVRICEGANSVPESEEL 420

Query: 421 GKVIAESLSGDSPVKMKAKALKREAVEAVRPNGSSWKDMQAFVNMLIQLP 471
           GKVIAESLS DS  KMKAKALKREAV AVRPNGSSWKDMQAF+  LIQLP
Sbjct: 421 GKVIAESLSRDSSEKMKAKALKREAVGAVRPNGSSWKDMQAFIYKLIQLP 470

BLAST of Lsi04G003350 vs. NCBI nr
Match: XP_038892836.1 (UDP-glycosyltransferase 89A2-like isoform X1 [Benincasa hispida] >XP_038892837.1 UDP-glycosyltransferase 89A2-like isoform X2 [Benincasa hispida])

HSP 1 Score: 760.0 bits (1961), Expect = 1.2e-215
Identity = 371/473 (78.44%), Postives = 408/473 (86.26%), Query Frame = 0

Query: 1   MSSSPPSSATSRHLLVFPYPAQGHMIALLDLTHHLASHGGFTITLLVTPKNLHLLQPLLQ 60
           MSSSPP S T RHLLVFP+PAQGHM+ LLDLTHHLASHGGFTIT+LVTPKNL +L PLL 
Sbjct: 1   MSSSPPPSTTRRHLLVFPFPAQGHMLPLLDLTHHLASHGGFTITILVTPKNLSVLHPLLS 60

Query: 61  TH--PSVQTLVLPLPSHPKLPVGVEHVAHIGNHGNFPIMAALRQLHDPIVEWFNSHPSPP 120
            H   SV+TLVLPLP HPKLP GVE++  IGNHGN PI+ ALRQLHDPIVEWFNSH SPP
Sbjct: 61  AHSSDSVRTLVLPLPHHPKLPNGVENIKDIGNHGNVPILLALRQLHDPIVEWFNSHSSPP 120

Query: 121 VAIISDFFLGWTQRLADHLRIPRVVFHSIATLLVHVMNSCWVQIKTDHFRSSPVIQFSEI 180
            AIISDFFLGWTQ LAD L+IPR+VF+S    LV+VM+ CW+  KT + RSS  I    I
Sbjct: 121 NAIISDFFLGWTQSLADRLQIPRIVFYSSGAFLVNVMDYCWIHTKTTNLRSS--ITIPNI 180

Query: 181 PKSPSFRKEHLPSLVRLYQDSDPDWNLLRDDALGNTSSWACVVNTFDSLEHEYLEHLRKL 240
           P SPSF +EH   +   YQDSDPDW LLRDDA+ N +SW  V NTF++LEH+YLEHLRK 
Sbjct: 181 PNSPSFEREHWSFMSLKYQDSDPDWVLLRDDAMANANSWGSVFNTFENLEHDYLEHLRKE 240

Query: 241 RGEGRVFGIGPLDIIGAAEDGRNPIRESSSEILTWLDRCPDDSVLYVCFGSQKQLNQQQM 300
           RGEGRVFG+GPL++IG  +DGRNPIR+SS ++LTWLD CPDDSV+YVCFGSQK+LN+QQM
Sbjct: 241 RGEGRVFGVGPLNLIG-DKDGRNPIRDSSLKVLTWLDGCPDDSVVYVCFGSQKKLNRQQM 300

Query: 301 EALASGLEKSGTRFVWVVKTFHQTDGGSDGIPIGFEDRVSDRGILAKGWAPQVSILNHRA 360
           EALASGLEKSGTRFVWVV+T HQTDGG +GIPIGFEDRVSDRGIL KGWAPQV+ILNHRA
Sbjct: 301 EALASGLEKSGTRFVWVVQTIHQTDGGFNGIPIGFEDRVSDRGILVKGWAPQVAILNHRA 360

Query: 361 VGGFLSHCGWNSVLESIVGGVMILGWPMEADQFINARLLVEELGVAVRVCEGANSVPESD 420
           VGGFLSHCGWNSVLESIVGGVMILGWPMEADQFINARLLVEELGVAVRVCEG NSVPESD
Sbjct: 361 VGGFLSHCGWNSVLESIVGGVMILGWPMEADQFINARLLVEELGVAVRVCEGTNSVPESD 420

Query: 421 ELGKVIAESLSGDSPVKMKAKALKREAVEAVRPNGSSWKDMQAFVNMLIQLPV 472
           ELGKVIAES+SGDSP KMKAKALKREAVEAVR NGSSWKDMQ  VN LI LP+
Sbjct: 421 ELGKVIAESMSGDSPEKMKAKALKREAVEAVRSNGSSWKDMQDLVNKLIHLPL 470

BLAST of Lsi04G003350 vs. NCBI nr
Match: XP_022967969.1 (UDP-glycosyltransferase 89A2-like [Cucurbita maxima])

HSP 1 Score: 753.4 bits (1944), Expect = 1.1e-213
Identity = 360/470 (76.60%), Postives = 410/470 (87.23%), Query Frame = 0

Query: 1   MSSSPPSSATSRHLLVFPYPAQGHMIALLDLTHHLASHGGFTITLLVTPKNLHLLQPLLQ 60
           MSSSPPS+ T+ HLLVFPYPAQGHMI+LLDLTH+LASHGGFTIT+LVTPKNL LLQPLLQ
Sbjct: 1   MSSSPPST-TTPHLLVFPYPAQGHMISLLDLTHYLASHGGFTITVLVTPKNLPLLQPLLQ 60

Query: 61  THPSVQTLVLPLPSHPKLPVGVEHVAHIGNHGNFPIMAALRQLHDPIVEWFNSHPSPPVA 120
            HPS+ TLVLP P+HP LP G EHV+ IGNHGNFPIM ALRQLHDPIV WFNSHP+PP A
Sbjct: 61  AHPSIHTLVLPFPAHPNLPAGAEHVSQIGNHGNFPIMVALRQLHDPIVHWFNSHPAPPAA 120

Query: 121 IISDFFLGWTQRLADHLRIPRVVFHSIATLLVHVMNSCWVQIKTDHFRSSPVIQFSEIPK 180
           IISDFFLGWTQ LADHLRIPR+V++S    LV+V++  W+ IKTD FRSSPV  F+EIP 
Sbjct: 121 IISDFFLGWTQHLADHLRIPRIVYYSCGAFLVNVLHYSWLHIKTDLFRSSPVFHFNEIPN 180

Query: 181 SPSFRKEHLPSLVRLYQDSDPDWNLLRDDALGNTSSWACVVNTFDSLEHEYLEHLRKLRG 240
           SPSF+K+HLPSL++ YQDS+ DW+LLR+DAL NTS W  V N+F++LE E+LEH R L G
Sbjct: 181 SPSFKKDHLPSLIKRYQDSNLDWDLLRNDALANTSGWVSVFNSFETLEGEFLEHFRDLNG 240

Query: 241 EGRVFGIGPLDIIGAAEDGRNPIRESSSEILTWLDRCPDDSVLYVCFGSQKQLNQQQMEA 300
           EGRVFGIGPL++IGA  DGR+ IR+SSSE+LTWLD C D SVLYVCFGSQKQLNQQQM+A
Sbjct: 241 EGRVFGIGPLNLIGAG-DGRSSIRDSSSEVLTWLDGCADSSVLYVCFGSQKQLNQQQMKA 300

Query: 301 LASGLEKSGTRFVWVVKTFHQTDGGSDGIPIGFEDRVSDRGILAKGWAPQVSILNHRAVG 360
           LASGLEKSG RFVWVVKTF+QT GG + IPIGFEDRVSDRGI+ K W PQV+ILNH+AVG
Sbjct: 301 LASGLEKSGARFVWVVKTFNQTVGGCNEIPIGFEDRVSDRGIVVKEWVPQVAILNHQAVG 360

Query: 361 GFLSHCGWNSVLESIVGGVMILGWPMEADQFINARLLVEELGVAVRVCEGANSVPESDEL 420
           GFLSHCGWNSVLES+  GV+ILGWPMEADQF+NA+LLV+++GVAVRVCEGANSVP+SDEL
Sbjct: 361 GFLSHCGWNSVLESVTAGVLILGWPMEADQFVNAKLLVDDMGVAVRVCEGANSVPDSDEL 420

Query: 421 GKVIAESLSGDSPVKMKAKALKREAVEAVRPNGSSWKDMQAFVNMLIQLP 471
           GKVIA SLSGD P KMKA ALKR AV+AVR NGSSWKDM+ FV+ L QLP
Sbjct: 421 GKVIAMSLSGDRPEKMKAGALKRAAVDAVRSNGSSWKDMRGFVDKLNQLP 468

BLAST of Lsi04G003350 vs. TAIR 10
Match: AT5G03490.1 (UDP-Glycosyltransferase superfamily protein )

HSP 1 Score: 461.1 bits (1185), Expect = 1.1e-129
Identity = 242/466 (51.93%), Postives = 319/466 (68.45%), Query Frame = 0

Query: 2   SSSPPSSATSRHLLVFPYPAQGHMIALLDLTHHLASHGGFTITLLVTPKNLHLLQPLLQT 61
           +S PP      H++VFP+PAQGH++ LLDLTH L    GF ++++VTP NL  L PLL  
Sbjct: 14  NSKPP------HIVVFPFPAQGHLLPLLDLTHQLCLR-GFNVSVIVTPGNLTYLSPLLSA 73

Query: 62  HP-SVQTLVLPLPSHPKLPVGVEHVAHIGNHGNFPIMAALRQLHDPIVEWFNSHPSPPVA 121
           HP SV ++V P P HP L  GVE+V  +GN GN PIMA+LRQL +PI+ WF SHP+PP+A
Sbjct: 74  HPSSVTSVVFPFPPHPSLSPGVENVKDVGNSGNLPIMASLRQLREPIINWFQSHPNPPIA 133

Query: 122 IISDFFLGWTQRLADHLRIPRVVFHSIATLLVHVMNSCWVQIKTDHFRSSPVIQFSEIPK 181
           +ISDFFLGWT  L + + IPR  F SI+  LV V+  C+  I  D  +S+  I   ++P+
Sbjct: 134 LISDFFLGWTHDLCNQIGIPRFAFFSISFFLVSVLQFCFENI--DLIKSTDPIHLLDLPR 193

Query: 182 SPSFRKEHLPSLV-RLYQDSDPDWNLLRDDALGNTSSWACVVNTFDSLEHEYLEHLRKLR 241
           +P F++EHLPS+V R  Q   PD   ++D ++ N  S+  V N+ + LE +YL+++++  
Sbjct: 194 APIFKEEHLPSIVRRSLQTPSPDLESIKDFSM-NLLSYGSVFNSSEILEDDYLQYVKQRM 253

Query: 242 GEGRVFGIGPLDIIGAAEDGRNPIRESSSEILTWLDRCPDDSVLYVCFGSQKQLNQQQME 301
           G  RV+ IGPL  IG+     +   + S  +L+WLD  P+ SVLYVCFGSQK L + Q +
Sbjct: 254 GHDRVYVIGPLCSIGSGLKSNSGSVDPS--LLSWLDGSPNGSVLYVCFGSQKALTKDQCD 313

Query: 302 ALASGLEKSGTRFVWVVKTFHQTDGGSDGIPIGFEDRVSDRGILAKGWAPQVSILNHRAV 361
           ALA GLEKS TRFVWVVK         D IP GFEDRVS RG++ +GW  Q+++L H AV
Sbjct: 314 ALALGLEKSMTRFVWVVK--------KDPIPDGFEDRVSGRGLVVRGWVSQLAVLRHVAV 373

Query: 362 GGFLSHCGWNSVLESIVGGVMILGWPMEADQFINARLLVEELGVAVRVCEGANSVPESDE 421
           GGFLSHCGWNSVLE I  G +ILGWPMEADQF+NARLLVE LGVAVRVCEG  +VP+SDE
Sbjct: 374 GGFLSHCGWNSVLEGITSGAVILGWPMEADQFVNARLLVEHLGVAVRVCEGGETVPDSDE 433

Query: 422 LGKVIAESL-SGDSPVKMKAKALKREAVEAV-RPNGSSWKDMQAFV 464
           LG+VIAE++  G   V  +A+ ++R+   AV   NGSS +++Q  V
Sbjct: 434 LGRVIAETMGEGGREVAARAEEIRRKTEAAVTEANGSSVENVQRLV 459

BLAST of Lsi04G003350 vs. TAIR 10
Match: AT1G73880.1 (UDP-glucosyl transferase 89B1 )

HSP 1 Score: 446.4 bits (1147), Expect = 2.8e-125
Identity = 220/466 (47.21%), Postives = 307/466 (65.88%), Query Frame = 0

Query: 10  TSRHLLVFPYPAQGHMIALLDLTHHLASHGG--FTITLLVTPKNLHLLQPLLQTHPSVQT 69
           T  H+L+FP+PAQGHMI LLD TH LA  GG    IT+LVTPKNL  L PLL    +++ 
Sbjct: 11  TKTHVLIFPFPAQGHMIPLLDFTHRLALRGGAALKITVLVTPKNLPFLSPLLSAVVNIEP 70

Query: 70  LVLPLPSHPKLPVGVEHVAHIGNHGNFPIMA-ALRQLHDPIVEWFNSHPSPPVAIISDFF 129
           L+LP PSHP +P GVE+V  +   G FP+M  AL  LH P++ W  SHPSPPVAI+SDFF
Sbjct: 71  LILPFPSHPSIPSGVENVQDLPPSG-FPLMIHALGNLHAPLISWITSHPSPPVAIVSDFF 130

Query: 130 LGWTQRLADHLRIPRVVFHSIATLLVHVMNSCWVQI--KTDHFRSSPVIQFSEIPKSPSF 189
           LGWT+ L     IPR  F   A +   ++N+ W+++  K +    + ++ F +IP  P +
Sbjct: 131 LGWTKNLG----IPRFDFSPSAAITCCILNTLWIEMPTKINEDDDNEILHFPKIPNCPKY 190

Query: 190 RKEHLPSLVRLYQDSDPDWNLLRDDALGNTSSWACVVNTFDSLEHEYLEHLRKLRGEGRV 249
           R + + SL R Y   DP W  +RD    N +SW  VVN+F ++E  YLEHL++  G  RV
Sbjct: 191 RFDQISSLYRSYVHGDPAWEFIRDSFRDNVASWGLVVNSFTAMEGVYLEHLKREMGHDRV 250

Query: 250 FGIGPLDIIGAAEDGR-NPIRESSSEILTWLDRCPDDSVLYVCFGSQKQLNQQQMEALAS 309
           + +GP  II  + D R  P   S   +++WLD   D+ V+YVCFGSQ  L ++Q  ALAS
Sbjct: 251 WAVGP--IIPLSGDNRGGPTSVSVDHVMSWLDAREDNHVVYVCFGSQVVLTKEQTLALAS 310

Query: 310 GLEKSGTRFVWVVKTFHQTDGGSDGIPIGFEDRVSDRGILAKGWAPQVSILNHRAVGGFL 369
           GLEKSG  F+W VK   + D     I  GF+DRV+ RG++ +GWAPQV++L HRAVG FL
Sbjct: 311 GLEKSGVHFIWAVKEPVEKDSTRGNILDGFDDRVAGRGLVIRGWAPQVAVLRHRAVGAFL 370

Query: 370 SHCGWNSVLESIVGGVMILGWPMEADQFINARLLVEELGVAVRVCEGANSVPESDELGKV 429
           +HCGWNSV+E++V GV++L WPM ADQ+ +A L+V+EL V VR CEG ++VP+ DEL +V
Sbjct: 371 THCGWNSVVEAVVAGVLMLTWPMRADQYTDASLVVDELKVGVRACEGPDTVPDPDELARV 430

Query: 430 IAESLSGDSPVKMKAKALKREAVEAVRPNGSSWKDMQAFVNMLIQL 470
            A+S++G+   ++KA  L++ A++A++  GSS  D+  F+  ++ L
Sbjct: 431 FADSVTGNQTERIKAVELRKAALDAIQERGSSVNDLDGFIQHVVSL 469

BLAST of Lsi04G003350 vs. TAIR 10
Match: AT1G51210.1 (UDP-Glycosyltransferase superfamily protein )

HSP 1 Score: 438.7 bits (1127), Expect = 5.8e-123
Identity = 220/429 (51.28%), Postives = 300/429 (69.93%), Query Frame = 0

Query: 13  HLLVFPYPAQGHMIALLDLTHHLASHGGFTITLLVTPKNLHLLQPLLQTHPS-VQTLVLP 72
           H++VFPYPAQGH++ LLDLTH L    G T++++VTPKNL  L PLL  HPS V  + LP
Sbjct: 20  HIMVFPYPAQGHLLPLLDLTHQLCLR-GLTVSIIVTPKNLPYLSPLLSAHPSAVSVVTLP 79

Query: 73  LPSHPKLPVGVEHVAHIGNHGNFPIMAALRQLHDPIVEWFNSHPSPPVAIISDFFLGWTQ 132
            P HP +P GVE+V  +G +GN  IMA+LRQL +PIV W +SHP+PPVA+ISDFFLGWT+
Sbjct: 80  FPHHPLIPSGVENVKDLGGYGNPLIMASLRQLREPIVNWLSSHPNPPVALISDFFLGWTK 139

Query: 133 RLADHLRIPRVVFHSIATLLVHVMNSCWVQIKTDHFRSSPVIQFSEIPKSPSFRKEHLPS 192
            L     IPR  F S    L  +++  +V  K   F S+  +  S++P+SP F+ EHLPS
Sbjct: 140 DLG----IPRFAFFSSGAFLASILH--FVSDKPHLFESTEPVCLSDLPRSPVFKTEHLPS 199

Query: 193 LVRLYQDSDPDWNLLRDDALGNTSSWACVVNTFDSLEHEYLEHLRKLRGEGRVFGIGPLD 252
           L+        D   ++D  + N SS+ C+ NT + LE +Y+E++++   E RVFG+GPL 
Sbjct: 200 LIP-QSPLSQDLESVKDSTM-NFSSYGCIFNTCECLEEDYMEYVKQKVSENRVFGVGPLS 259

Query: 253 IIGAAEDGRNPIRESSSEILTWLDRCPDDSVLYVCFGSQKQLNQQQMEALASGLEKSGTR 312
            +G +++  +     +  +L+WLD CPDDSVLY+CFGSQK L ++Q + LA GLEKS TR
Sbjct: 260 SVGLSKED-SVSNVDAKALLSWLDGCPDDSVLYICFGSQKVLTKEQCDDLALGLEKSMTR 319

Query: 313 FVWVVKTFHQTDGGSDGIPIGFEDRVSDRGILAKGWAPQVSILNHRAVGGFLSHCGWNSV 372
           FVWVVK         D IP GFEDRV+ RG++ +GWAPQV++L+H AVGGFL HCGWNSV
Sbjct: 320 FVWVVK--------KDPIPDGFEDRVAGRGMIVRGWAPQVAMLSHVAVGGFLIHCGWNSV 379

Query: 373 LESIVGGVMILGWPMEADQFINARLLVEELGVAVRVCEGANSVPESDELGKVIAESLSGD 432
           LE++  G MIL WPMEADQF++ARL+VE +GVAV VCEG  +VP+  E+G++IA+++ G+
Sbjct: 380 LEAMASGTMILAWPMEADQFVDARLVVEHMGVAVSVCEGGKTVPDPYEMGRIIADTM-GE 429

Query: 433 SPVKMKAKA 441
           S  + +A+A
Sbjct: 440 SGGEARARA 429

BLAST of Lsi04G003350 vs. TAIR 10
Match: AT1G06000.1 (UDP-Glycosyltransferase superfamily protein )

HSP 1 Score: 302.8 bits (774), Expect = 5.0e-82
Identity = 175/464 (37.72%), Postives = 250/464 (53.88%), Query Frame = 0

Query: 13  HLLVFPYPAQGHMIALLDLTHHLASHGGFTITLLVTPKNLHLLQPLLQTH--PSVQTLVL 72
           H+LV P+P  GHM+  LDLTH +   G  T+T+LVTPKN   L  L   H     +TL+L
Sbjct: 10  HVLVIPFPQSGHMVPHLDLTHQILLRGA-TVTVLVTPKNSSYLDALRSLHSPEHFKTLIL 69

Query: 73  PLPSHPKLPVGVEHVAHIGNHGNFPIMAALRQLHDPIVEWFNSHPSP--PVAIISDFFLG 132
           P PSHP +P GVE +  +       +  AL +LHDP+V++ +  P    P AI+   FL 
Sbjct: 70  PFPSHPCIPSGVESLQQLPLEAIVHMFDALSRLHDPLVDFLSRQPPSDLPDAILGSSFLS 129

Query: 133 -WTQRLADHLRIPRVVFHSIATLLVHVMNSCWVQIKTDHFRSSPVIQFSEIPKSPSFRKE 192
            W  ++AD   I  + F  I    + VM   W Q                          
Sbjct: 130 PWINKVADAFSIKSISFLPINAHSISVM---WAQ-------------------------- 189

Query: 193 HLPSLVRLYQDSDPDWNLLRDDALGNTSSWACVVNTFDSLEHEYLEHLR-KLRGEGRVFG 252
                         D +   D     T S+  V+N+F  LE E++E ++ +     R++ 
Sbjct: 190 -------------EDRSFFNDLETATTESYGLVINSFYDLEPEFVETVKTRFLNHHRIWT 249

Query: 253 IGPLDIIGAAEDGRNPIRESSSEILTWLDRCPDD-SVLYVCFGSQKQLNQQQMEALASGL 312
           +GPL    A  D         +++  WLD CP+D SV+YV FGSQ +L  +Q  ALA+ L
Sbjct: 250 VGPLLPFKAGVDRGGQSSIPPAKVSAWLDSCPEDNSVVYVGFGSQIRLTAEQTAALAAAL 309

Query: 313 EKSGTRFVWVVKTFHQTDGGSDG------IPIGFEDRVSDRGILAKGWAPQVSILNHRAV 372
           EKS  RF+W V+   +    SD       IP GFE+RV ++G++ +GWAPQ  IL HRAV
Sbjct: 310 EKSSVRFIWAVRDAAKKVNSSDNSVEEDVIPAGFEERVKEKGLVIRGWAPQTMILEHRAV 369

Query: 373 GGFLSHCGWNSVLESIVGGVMILGWPMEADQFINARLLVEELGVAVRVCEGANSVPESDE 432
           G +L+H GW SVLE +VGGVM+L WPM+AD F N  L+V++L  AVRV E  +SVP+SD+
Sbjct: 370 GSYLTHLGWGSVLEGMVGGVMLLAWPMQADHFFNTTLIVDKLRAAVRVGENRDSVPDSDK 429

Query: 433 LGKVIAESLSGDSPVKMKAKALKREAVEAVRPNGSSWKDMQAFV 464
           L +++AES   D P ++    L+ +A+EA++  GSS+K++   V
Sbjct: 430 LARILAESAREDLPERVTLMKLREKAMEAIKEGGSSYKNLDELV 430

BLAST of Lsi04G003350 vs. TAIR 10
Match: AT2G36780.1 (UDP-Glycosyltransferase superfamily protein )

HSP 1 Score: 232.3 bits (591), Expect = 8.3e-61
Identity = 157/489 (32.11%), Postives = 246/489 (50.31%), Query Frame = 0

Query: 11  SRHLLVFPYPAQGHMIALLDLTHHLASHGGFTITLLVTPKNL----HLLQPLLQTHPSVQ 70
           S H ++FP+ AQGHMI ++D+   LA   G TIT++ TP N     ++L   +++  ++ 
Sbjct: 12  SLHFVLFPFMAQGHMIPMIDIARLLAQR-GVTITIVTTPHNAARFKNVLNRAIESGLAIN 71

Query: 71  TLVLPLPSHP-KLPVGVEHVAHI-GNHGNFPIMAALRQLHDPIVEWFNSHPSPPVAIISD 130
            L +  P     LP G E++  +       P   A+  L DP+++        P  +ISD
Sbjct: 72  ILHVKFPYQEFGLPEGKENIDSLDSTELMVPFFKAVNLLEDPVMKLMEEMKPRPSCLISD 131

Query: 131 FFLGWTQRLADHLRIPRVVFHSIA---TLLVHVMNSCWVQIKTDHFRSSPVIQFSEIPKS 190
           + L +T  +A +  IP++VFH +     L +HV+    ++I  +             P  
Sbjct: 132 WCLPYTSIIAKNFNIPKIVFHGMGCFNLLCMHVLRRN-LEILENVKSDEEYFLVPSFPDR 191

Query: 191 PSFRKEHLPSLVRLYQDSDPDWNLLRDDAL-GNTSSWACVVNTFDSLEHEYLEHLRKLRG 250
             F K  LP    +  ++  DW  + D+ +    +S+  +VNTF  LE  Y++  ++   
Sbjct: 192 VEFTKLQLP----VKANASGDWKEIMDEMVKAEYTSYGVIVNTFQELEPPYVKDYKEAM- 251

Query: 251 EGRVFGIGPLDIIGAAEDGRNPIRESS------SEILTWLDRCPDDSVLYVCFGSQKQLN 310
           +G+V+ IGP+ +   A  G +     S       E L WLD   + SVLYVC GS   L 
Sbjct: 252 DGKVWSIGPVSLCNKA--GADKAERGSKAAIDQDECLQWLDSKEEGSVLYVCLGSICNLP 311

Query: 311 QQQMEALASGLEKSGTRFVWVVKTFHQTDGGSDG-IPIGFEDRVSDRGILAKGWAPQVSI 370
             Q++ L  GLE+S   F+WV++   +     +  +  GFE+R+ +RG+L KGWAPQV I
Sbjct: 312 LSQLKELGLGLEESRRSFIWVIRGSEKYKELFEWMLESGFEERIKERGLLIKGWAPQVLI 371

Query: 371 LNHRAVGGFLSHCGWNSVLESIVGGVMILGWPMEADQFINARLLVEELGVAVRV-CEGAN 430
           L+H +VGGFL+HCGWNS LE I  G+ ++ WP+  DQF N +L+V+ L   V    E   
Sbjct: 372 LSHPSVGGFLTHCGWNSTLEGITSGIPLITWPLFGDQFCNQKLVVQVLKAGVSAGVEEVM 431

Query: 431 SVPESDELG--------KVIAESLSGDS----PVKMKAKALKREAVEAVRPNGSSWKDMQ 470
              E D++G        K   E L GDS      + + K L   A +AV   GSS  ++ 
Sbjct: 432 KWGEEDKIGVLVDKEGVKKAVEELMGDSDDAKERRRRVKELGELAHKAVEKGGSSHSNIT 491

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LZD81.5e-12851.93UDP-glycosyltransferase 89A2 OS=Arabidopsis thaliana OX=3702 GN=UGT89A2 PE=2 SV=... [more]
Q9C9B03.9e-12447.21Flavonol 3-O-glucosyltransferase UGT89B1 OS=Arabidopsis thaliana OX=3702 GN=UGT8... [more]
Q6VAA51.7e-11447.39UDP-glycosyltransferase 89B2 OS=Stevia rebaudiana OX=55670 GN=UGT89B2 PE=2 SV=1[more]
Q9LNE67.0e-8137.72Flavonol 7-O-rhamnosyltransferase OS=Arabidopsis thaliana OX=3702 GN=UGT89C1 PE=... [more]
K4GMD61.9e-6233.60UDP-glycosyltransferase 73C9 OS=Barbarea vulgaris OX=50459 GN=UGT73C9 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0LCK32.9e-23983.83Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G640640 PE=4 SV=1[more]
A0A1S3CC758.8e-23683.83UDP-glycosyltransferase 89A2-like OS=Cucumis melo OX=3656 GN=LOC103499149 PE=4 S... [more]
A0A6J1HY915.5e-21476.60UDP-glycosyltransferase 89A2-like OS=Cucurbita maxima OX=3661 GN=LOC111467348 PE... [more]
A0A6J1FZE52.1e-21375.90UDP-glycosyltransferase 89A2-like OS=Cucurbita moschata OX=3662 GN=LOC111449303 ... [more]
A0A346A6C14.1e-20976.63Flavonoids UDP-glycosyltransferase OS=Siraitia grosvenorii OX=190515 GN=UGT89M1 ... [more]
Match NameE-valueIdentityDescription
XP_038893299.17.3e-25389.83UDP-glycosyltransferase 89A2-like [Benincasa hispida][more]
XP_031739347.16.0e-23983.83UDP-glycosyltransferase 89A2 [Cucumis sativus] >KAE8650874.1 hypothetical protei... [more]
XP_008460270.11.8e-23583.83PREDICTED: UDP-glycosyltransferase 89A2-like [Cucumis melo][more]
XP_038892836.11.2e-21578.44UDP-glycosyltransferase 89A2-like isoform X1 [Benincasa hispida] >XP_038892837.1... [more]
XP_022967969.11.1e-21376.60UDP-glycosyltransferase 89A2-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
AT5G03490.11.1e-12951.93UDP-Glycosyltransferase superfamily protein [more]
AT1G73880.12.8e-12547.21UDP-glucosyl transferase 89B1 [more]
AT1G51210.15.8e-12351.28UDP-Glycosyltransferase superfamily protein [more]
AT1G06000.15.0e-8237.72UDP-Glycosyltransferase superfamily protein [more]
AT2G36780.18.3e-6132.11UDP-Glycosyltransferase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 471..472
NoneNo IPR availableGENE3D3.40.50.2000Glycogen Phosphorylase B;coord: 268..450
e-value: 8.2E-112
score: 376.4
NoneNo IPR availableGENE3D3.40.50.2000Glycogen Phosphorylase B;coord: 13..455
e-value: 8.2E-112
score: 376.4
NoneNo IPR availablePANTHERPTHR48047:SF28UDP-GLYCOSYLTRANSFERASE 89A2coord: 10..469
NoneNo IPR availablePANTHERPTHR48047GLYCOSYLTRANSFERASEcoord: 10..469
NoneNo IPR availableSUPERFAMILY53756UDP-Glycosyltransferase/glycogen phosphorylasecoord: 12..468
IPR002213UDP-glucuronosyl/UDP-glucosyltransferasePFAMPF00201UDPGTcoord: 269..408
e-value: 5.1E-16
score: 58.5
IPR002213UDP-glucuronosyl/UDP-glucosyltransferaseCDDcd03784GT1_Gtf-likecoord: 13..448
e-value: 6.29464E-64
score: 210.485

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi04G003350.1Lsi04G003350.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0009507 chloroplast
molecular_function GO:0008194 UDP-glycosyltransferase activity