Lsi04G003100 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi04G003100
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	Calmodulin, putative
Location	chr04: 3135421 .. 3135906 (-)
RNA-Seq Expression	Lsi04G003100
Synteny	Lsi04G003100

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCTTCCATTCAATCCGACCAGCAGCTCAAGCAGCTGCACGACATTTTCAGGCGTTTCGACATGAACTCCGACGGCAGCCTCACGCAGCTCGAACTCGGCGCCCTTCTCCGCTCCCTCGGCATCAAACCCTCCGGCGACCAACTCCATTCTCTCCTCTCCAACATGGATTCTAACGGCAACGGCTCCATCGAATTCGATGAACTCGTCAATGCCATTCTCCCCGATATGAACGACGATATTCTCGTCAATCAAGAGCAGTTGTTGGAGGTTTTCAGATCTTTCGATCGCGATGGCAATGGCTACATCACCGCTGCGGAGCTCGCTGGTTCCATGGCGAAGATGGGACATCCTCTGACCTACCGCGAACTCTCCGAGATGATGCGACAGGCCGATACCGACGGCGACGGCGTAATTAGCTTCAACGAATTCACCACCGTGATGGCCAAATCCGCCGCGGATTTTCTCGGCCTTACATTTGCGTAG

mRNA sequence

ATGGCTTCCATTCAATCCGACCAGCAGCTCAAGCAGCTGCACGACATTTTCAGGCGTTTCGACATGAACTCCGACGGCAGCCTCACGCAGCTCGAACTCGGCGCCCTTCTCCGCTCCCTCGGCATCAAACCCTCCGGCGACCAACTCCATTCTCTCCTCTCCAACATGGATTCTAACGGCAACGGCTCCATCGAATTCGATGAACTCGTCAATGCCATTCTCCCCGATATGAACGACGATATTCTCGTCAATCAAGAGCAGTTGTTGGAGGTTTTCAGATCTTTCGATCGCGATGGCAATGGCTACATCACCGCTGCGGAGCTCGCTGGTTCCATGGCGAAGATGGGACATCCTCTGACCTACCGCGAACTCTCCGAGATGATGCGACAGGCCGATACCGACGGCGACGGCGTAATTAGCTTCAACGAATTCACCACCGTGATGGCCAAATCCGCCGCGGATTTTCTCGGCCTTACATTTGCGTAG

Coding sequence (CDS)

ATGGCTTCCATTCAATCCGACCAGCAGCTCAAGCAGCTGCACGACATTTTCAGGCGTTTCGACATGAACTCCGACGGCAGCCTCACGCAGCTCGAACTCGGCGCCCTTCTCCGCTCCCTCGGCATCAAACCCTCCGGCGACCAACTCCATTCTCTCCTCTCCAACATGGATTCTAACGGCAACGGCTCCATCGAATTCGATGAACTCGTCAATGCCATTCTCCCCGATATGAACGACGATATTCTCGTCAATCAAGAGCAGTTGTTGGAGGTTTTCAGATCTTTCGATCGCGATGGCAATGGCTACATCACCGCTGCGGAGCTCGCTGGTTCCATGGCGAAGATGGGACATCCTCTGACCTACCGCGAACTCTCCGAGATGATGCGACAGGCCGATACCGACGGCGACGGCGTAATTAGCTTCAACGAATTCACCACCGTGATGGCCAAATCCGCCGCGGATTTTCTCGGCCTTACATTTGCGTAG

Protein sequence

MASIQSDQQLKQLHDIFRRFDMNSDGSLTQLELGALLRSLGIKPSGDQLHSLLSNMDSNGNGSIEFDELVNAILPDMNDDILVNQEQLLEVFRSFDRDGNGYITAAELAGSMAKMGHPLTYRELSEMMRQADTDGDGVISFNEFTTVMAKSAADFLGLTFA

Homology

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BLAST of Lsi04G003100 vs. ExPASy Swiss-Prot
Match: Q9LI84 (Probable calcium-binding protein CML16 OS=Arabidopsis thaliana OX=3702 GN=CML16 PE=2 SV=2)

HSP 1 Score: 246.1 bits (627), Expect = 2.7e-64
Identity = 123/159 (77.36%), Postives = 142/159 (89.31%), Query Frame = 0


Query: 1   MASIQSDQQLKQLHDIFRRFDMNSDGSLTQLELGALLRSLGIKPSGDQLHSLLSNMDSNG 60
           MAS +   Q+KQL DIF RFDM+ DGSLTQLEL ALLRSLGIKP GDQ+  LL+ +D NG
Sbjct: 1   MASTKPTDQIKQLKDIFARFDMDKDGSLTQLELAALLRSLGIKPRGDQISLLLNQIDRNG 60

Query: 61  NGSIEFDELVNAILPDMNDDILVNQEQLLEVFRSFDRDGNGYITAAELAGSMAKMGHPLT 120
           NGS+EFDELV AILPD+N+++L+NQEQL+EVFRSFDRDGNG ITAAELAGSMAKMGHPLT
Sbjct: 61  NGSVEFDELVVAILPDINEEVLINQEQLMEVFRSFDRDGNGSITAAELAGSMAKMGHPLT 120

Query: 121 YRELSEMMRQADTDGDGVISFNEFTTVMAKSAADFLGLT 160
           YREL+EMM +AD++GDGVISFNEF+ +MAKSAADFLGLT
Sbjct: 121 YRELTEMMTEADSNGDGVISFNEFSHIMAKSAADFLGLT 159

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BLAST of Lsi04G003100 vs. ExPASy Swiss-Prot
Match: Q9FZ75 (Probable calcium-binding protein CML15 OS=Arabidopsis thaliana OX=3702 GN=CML15 PE=2 SV=1)

HSP 1 Score: 230.3 bits (586), Expect = 1.5e-59
Identity = 108/152 (71.05%), Postives = 137/152 (90.13%), Query Frame = 0


Query: 7   DQQLKQLHDIFRRFDMNSDGSLTQLELGALLRSLGIKPSGDQLHSLLSNMDSNGNGSIEF 66
           + Q++QL DIF RFDM++DGSLT LEL ALLRSLG+KPSGDQ+H LL++MDSNGNG +EF
Sbjct: 2   EDQIRQLKDIFDRFDMDADGSLTILELAALLRSLGLKPSGDQIHVLLASMDSNGNGFVEF 61

Query: 67  DELVNAILPDMNDDILVNQEQLLEVFRSFDRDGNGYITAAELAGSMAKMGHPLTYRELSE 126
           DELV  ILPD+N+++L+N EQLLE+F+SFDRDGNG+I+AAELAG+MAKMG PLTY+EL+E
Sbjct: 62  DELVGTILPDLNEEVLINSEQLLEIFKSFDRDGNGFISAAELAGAMAKMGQPLTYKELTE 121

Query: 127 MMRQADTDGDGVISFNEFTTVMAKSAADFLGL 159
           M+++ADT+GDGVISF EF ++MAKSA D+ GL
Sbjct: 122 MIKEADTNGDGVISFGEFASIMAKSAVDYFGL 153

[Close]

BLAST of Lsi04G003100 vs. ExPASy Swiss-Prot
Match: Q6L5F4 (Probable calcium-binding protein CML14 OS=Oryza sativa subsp. japonica OX=39947 GN=CML14 PE=2 SV=1)

HSP 1 Score: 213.8 bits (543), Expect = 1.5e-54
Identity = 107/151 (70.86%), Postives = 128/151 (84.77%), Query Frame = 0


Query: 9   QLKQLHDIFRRFDMNSDGSLTQLELGALLRSLGIKPSGDQLHSLLSNMDSNGNGSIEFDE 68
           QLKQL ++FRRFDMN DGSLTQLEL ALLRSLG++P+GD++H+LL+ MD+NGNGS+EFDE
Sbjct: 22  QLKQLRELFRRFDMNGDGSLTQLELAALLRSLGLRPTGDEVHALLAGMDANGNGSVEFDE 81

Query: 69  LVNAILPDMNDDI-LVNQEQLLEVFRSFDRDGNGYITAAELAGSMAKMGHPLTYRELSEM 128
           L  AI P +     LV+Q QLLEVFR+FDRDGNG+I+AAELA SMA++G PLT+ EL+ M
Sbjct: 82  LAAAIAPVLTTQTHLVDQAQLLEVFRAFDRDGNGFISAAELARSMARLGQPLTFEELTRM 141

Query: 129 MRQADTDGDGVISFNEFTTVMAKSAADFLGL 159
           MR ADTDGDGVISF EF  VMAKSA DFLG+
Sbjct: 142 MRDADTDGDGVISFKEFAAVMAKSALDFLGV 172

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BLAST of Lsi04G003100 vs. ExPASy Swiss-Prot
Match: Q5ZD81 (Probable calcium-binding protein CML12 OS=Oryza sativa subsp. japonica OX=39947 GN=CML12 PE=2 SV=1)

HSP 1 Score: 196.8 bits (499), Expect = 1.8e-49
Identity = 96/156 (61.54%), Postives = 130/156 (83.33%), Query Frame = 0


Query: 8   QQLKQLHDIFRRFDMNSDGSLTQLELGALLRSLGIKP-SGDQLHSLLSNMDSNGNGSIEF 67
           +QL+QLH+IF RFD++ DGSLT+LEL ALLRSLG++P +GD++H+L++ +D++GNG++EF
Sbjct: 91  EQLRQLHEIFLRFDLDGDGSLTKLELAALLRSLGLRPAAGDEIHALIAAIDADGNGTVEF 150

Query: 68  DELVNA----ILPDMNDDILVNQEQLLEVFRSFDRDGNGYITAAELAGSMAKMGHPLTYR 127
           DEL ++    IL      + V+Q +L E FR+FDRDGNG+I+AAELA SMA+MGHP+ Y 
Sbjct: 151 DELASSLADLILGPCRPSVAVDQAELAEAFRAFDRDGNGFISAAELARSMARMGHPICYA 210

Query: 128 ELSEMMRQADTDGDGVISFNEFTTVMAKSAADFLGL 159
           EL++MMR+ADTDGDG+ISF EFT +MAKSA DFLGL
Sbjct: 211 ELTDMMREADTDGDGLISFEEFTAIMAKSALDFLGL 246

[Close]

BLAST of Lsi04G003100 vs. ExPASy Swiss-Prot
Match: Q9AWK2 (Probable calcium-binding protein CML11 OS=Oryza sativa subsp. japonica OX=39947 GN=CML11 PE=2 SV=1)

HSP 1 Score: 166.0 bits (419), Expect = 3.5e-40
Identity = 85/149 (57.05%), Postives = 110/149 (73.83%), Query Frame = 0


Query: 7   DQQLKQLHDIFRRFDMNSDGSLTQLELGALLRSLGIKPSGDQLHSLLSNMDSNGNGSIEF 66
           D QL +L +IFR FD N DGSLTQLELG+LLRSLG+KPS D+L SL+   D+N NG IEF
Sbjct: 59  DDQLGELREIFRSFDRNGDGSLTQLELGSLLRSLGLKPSTDELDSLIQRADTNSNGLIEF 118

Query: 67  DELVNAILPD-MNDDILVNQEQLLEVFRSFDRDGNGYITAAELAGSMAKMGHPLTYRELS 126
            E V  + P+ + D    +++Q+  +F  FDRDGNG+ITAAELA SMAK+GH LT +EL+
Sbjct: 119 SEFVALVAPELLYDRAPYSEDQIRRLFNIFDRDGNGFITAAELAHSMAKLGHALTVKELT 178

Query: 127 EMMRQADTDGDGVISFNEFTTVMAKSAAD 155
            M+++ADTDGDG ISF EF+  +  +A D
Sbjct: 179 GMIKEADTDGDGRISFQEFSRAITAAAFD 207

[Close]

BLAST of Lsi04G003100 vs. ExPASy TrEMBL
Match: A0A1S3BF88 (probable calcium-binding protein CML16 OS=Cucumis melo OX=3656 GN=LOC103489229 PE=4 SV=1)

HSP 1 Score: 310.8 bits (795), Expect = 3.2e-81
Identity = 159/161 (98.76%), Postives = 160/161 (99.38%), Query Frame = 0


Query: 1   MASIQSDQQLKQLHDIFRRFDMNSDGSLTQLELGALLRSLGIKPSGDQLHSLLSNMDSNG 60
           MASIQSD QLKQLHDIFRRFDMNSDGSLTQLELGALLRSLGIKPSGDQLHSLLSNMDSNG
Sbjct: 1   MASIQSDHQLKQLHDIFRRFDMNSDGSLTQLELGALLRSLGIKPSGDQLHSLLSNMDSNG 60

Query: 61  NGSIEFDELVNAILPDMNDDILVNQEQLLEVFRSFDRDGNGYITAAELAGSMAKMGHPLT 120
           NGSIEFDELVNAILPDMNDDILVNQEQL+EVFRSFDRDGNGYITAAELAGSMAKMGHPLT
Sbjct: 61  NGSIEFDELVNAILPDMNDDILVNQEQLMEVFRSFDRDGNGYITAAELAGSMAKMGHPLT 120

Query: 121 YRELSEMMRQADTDGDGVISFNEFTTVMAKSAADFLGLTFA 162
           YRELSEMMRQADTDGDGVISFNEFTTVMAKSAADFLGLTFA
Sbjct: 121 YRELSEMMRQADTDGDGVISFNEFTTVMAKSAADFLGLTFA 161

[Close]

BLAST of Lsi04G003100 vs. ExPASy TrEMBL
Match: A0A0A0KUE0 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G608150 PE=4 SV=1)

HSP 1 Score: 309.7 bits (792), Expect = 7.2e-81
Identity = 158/161 (98.14%), Postives = 160/161 (99.38%), Query Frame = 0


Query: 1   MASIQSDQQLKQLHDIFRRFDMNSDGSLTQLELGALLRSLGIKPSGDQLHSLLSNMDSNG 60
           MASIQSD QLKQLHDIFRRFDMNSDGSLTQLELGALLRSLGIKPSGDQLHSLLSNMDSNG
Sbjct: 1   MASIQSDHQLKQLHDIFRRFDMNSDGSLTQLELGALLRSLGIKPSGDQLHSLLSNMDSNG 60

Query: 61  NGSIEFDELVNAILPDMNDDILVNQEQLLEVFRSFDRDGNGYITAAELAGSMAKMGHPLT 120
           NGSIEFDELVNAILPDMNDDILVNQEQL+EVFRSFDRDGNGYITAAELAGSMAKMGHPLT
Sbjct: 61  NGSIEFDELVNAILPDMNDDILVNQEQLMEVFRSFDRDGNGYITAAELAGSMAKMGHPLT 120

Query: 121 YRELSEMMRQADTDGDGVISFNEFTTVMAKSAADFLGLTFA 162
           YRELS+MMRQADTDGDGVISFNEFTTVMAKSAADFLGLTFA
Sbjct: 121 YRELSDMMRQADTDGDGVISFNEFTTVMAKSAADFLGLTFA 161

[Close]

BLAST of Lsi04G003100 vs. ExPASy TrEMBL
Match: A0A6J1JA36 (probable calcium-binding protein CML16 OS=Cucurbita maxima OX=3661 GN=LOC111484890 PE=4 SV=1)

HSP 1 Score: 295.8 bits (756), Expect = 1.1e-76
Identity = 151/161 (93.79%), Postives = 160/161 (99.38%), Query Frame = 0


Query: 1   MASIQSDQQLKQLHDIFRRFDMNSDGSLTQLELGALLRSLGIKPSGDQLHSLLSNMDSNG 60
           MAS+QSD QLKQLHDIFRRFDMNSDGSLTQLELGALLRSLGIKPSGDQLHSLLSNMDSNG
Sbjct: 1   MASMQSD-QLKQLHDIFRRFDMNSDGSLTQLELGALLRSLGIKPSGDQLHSLLSNMDSNG 60

Query: 61  NGSIEFDELVNAILPDMNDDILVNQEQLLEVFRSFDRDGNGYITAAELAGSMAKMGHPLT 120
           NGSIEFDELVNAILPD+ND+ILVNQEQL+EVF+SFDRDGNG+ITA+ELAGSMAKMGHPL+
Sbjct: 61  NGSIEFDELVNAILPDLNDNILVNQEQLMEVFKSFDRDGNGFITASELAGSMAKMGHPLS 120

Query: 121 YRELSEMMRQADTDGDGVISFNEFTTVMAKSAADFLGLTFA 162
           YRELSEMMRQADTDGDGVISFNEFTTVMA+SAADFLGLTFA
Sbjct: 121 YRELSEMMRQADTDGDGVISFNEFTTVMARSAADFLGLTFA 160

[Close]

BLAST of Lsi04G003100 vs. ExPASy TrEMBL
Match: A0A6J1GX12 (probable calcium-binding protein CML16 OS=Cucurbita moschata OX=3662 GN=LOC111458327 PE=4 SV=1)

HSP 1 Score: 294.7 bits (753), Expect = 2.4e-76
Identity = 150/161 (93.17%), Postives = 160/161 (99.38%), Query Frame = 0


Query: 1   MASIQSDQQLKQLHDIFRRFDMNSDGSLTQLELGALLRSLGIKPSGDQLHSLLSNMDSNG 60
           MAS+QSD QLKQLHDIFRRFDMNSDGSLTQLELGALLRSLGIKPSGDQLHSLLSNMDSNG
Sbjct: 1   MASLQSD-QLKQLHDIFRRFDMNSDGSLTQLELGALLRSLGIKPSGDQLHSLLSNMDSNG 60

Query: 61  NGSIEFDELVNAILPDMNDDILVNQEQLLEVFRSFDRDGNGYITAAELAGSMAKMGHPLT 120
           NGSIEF+ELVNAILPD+ND+ILVNQEQL+EVF+SFDRDGNG+ITA+ELAGSMAKMGHPL+
Sbjct: 61  NGSIEFEELVNAILPDLNDNILVNQEQLMEVFKSFDRDGNGFITASELAGSMAKMGHPLS 120

Query: 121 YRELSEMMRQADTDGDGVISFNEFTTVMAKSAADFLGLTFA 162
           YRELSEMMRQADTDGDGVISFNEFTTVMA+SAADFLGLTFA
Sbjct: 121 YRELSEMMRQADTDGDGVISFNEFTTVMARSAADFLGLTFA 160

[Close]

BLAST of Lsi04G003100 vs. ExPASy TrEMBL
Match: A0A6J1D725 (probable calcium-binding protein CML16 OS=Momordica charantia OX=3673 GN=LOC111017528 PE=4 SV=1)

HSP 1 Score: 292.0 bits (746), Expect = 1.6e-75
Identity = 153/161 (95.03%), Postives = 156/161 (96.89%), Query Frame = 0


Query: 1   MASIQSDQQLKQLHDIFRRFDMNSDGSLTQLELGALLRSLGIKPSGDQLHSLLSNMDSNG 60
           MASIQSD QLKQL DIFRRFDMNSDGSLTQLELGALLRSLGIKPSGDQLHSLLSNMDSNG
Sbjct: 1   MASIQSD-QLKQLQDIFRRFDMNSDGSLTQLELGALLRSLGIKPSGDQLHSLLSNMDSNG 60

Query: 61  NGSIEFDELVNAILPDMNDDILVNQEQLLEVFRSFDRDGNGYITAAELAGSMAKMGHPLT 120
           NGSIEF+ELVNAILPDMNDDILVNQEQLLEVFRSFDRDGNGYITA+ELAGSMAKMG PL+
Sbjct: 61  NGSIEFEELVNAILPDMNDDILVNQEQLLEVFRSFDRDGNGYITASELAGSMAKMGQPLS 120

Query: 121 YRELSEMMRQADTDGDGVISFNEFTTVMAKSAADFLGLTFA 162
           YRELSEMMR AD DGDGVISFNEFTTVMAKSAADFLGLTFA
Sbjct: 121 YRELSEMMRLADIDGDGVISFNEFTTVMAKSAADFLGLTFA 160

[Close]

BLAST of Lsi04G003100 vs. NCBI nr
Match: XP_008446524.1 (PREDICTED: probable calcium-binding protein CML16 [Cucumis melo])

HSP 1 Score: 310.8 bits (795), Expect = 6.7e-81
Identity = 159/161 (98.76%), Postives = 160/161 (99.38%), Query Frame = 0


Query: 1   MASIQSDQQLKQLHDIFRRFDMNSDGSLTQLELGALLRSLGIKPSGDQLHSLLSNMDSNG 60
           MASIQSD QLKQLHDIFRRFDMNSDGSLTQLELGALLRSLGIKPSGDQLHSLLSNMDSNG
Sbjct: 1   MASIQSDHQLKQLHDIFRRFDMNSDGSLTQLELGALLRSLGIKPSGDQLHSLLSNMDSNG 60

Query: 61  NGSIEFDELVNAILPDMNDDILVNQEQLLEVFRSFDRDGNGYITAAELAGSMAKMGHPLT 120
           NGSIEFDELVNAILPDMNDDILVNQEQL+EVFRSFDRDGNGYITAAELAGSMAKMGHPLT
Sbjct: 61  NGSIEFDELVNAILPDMNDDILVNQEQLMEVFRSFDRDGNGYITAAELAGSMAKMGHPLT 120

Query: 121 YRELSEMMRQADTDGDGVISFNEFTTVMAKSAADFLGLTFA 162
           YRELSEMMRQADTDGDGVISFNEFTTVMAKSAADFLGLTFA
Sbjct: 121 YRELSEMMRQADTDGDGVISFNEFTTVMAKSAADFLGLTFA 161

[Close]

BLAST of Lsi04G003100 vs. NCBI nr
Match: XP_004135127.1 (probable calcium-binding protein CML16 [Cucumis sativus] >KGN52027.1 hypothetical protein Csa_008611 [Cucumis sativus])

HSP 1 Score: 309.7 bits (792), Expect = 1.5e-80
Identity = 158/161 (98.14%), Postives = 160/161 (99.38%), Query Frame = 0


Query: 1   MASIQSDQQLKQLHDIFRRFDMNSDGSLTQLELGALLRSLGIKPSGDQLHSLLSNMDSNG 60
           MASIQSD QLKQLHDIFRRFDMNSDGSLTQLELGALLRSLGIKPSGDQLHSLLSNMDSNG
Sbjct: 1   MASIQSDHQLKQLHDIFRRFDMNSDGSLTQLELGALLRSLGIKPSGDQLHSLLSNMDSNG 60

Query: 61  NGSIEFDELVNAILPDMNDDILVNQEQLLEVFRSFDRDGNGYITAAELAGSMAKMGHPLT 120
           NGSIEFDELVNAILPDMNDDILVNQEQL+EVFRSFDRDGNGYITAAELAGSMAKMGHPLT
Sbjct: 61  NGSIEFDELVNAILPDMNDDILVNQEQLMEVFRSFDRDGNGYITAAELAGSMAKMGHPLT 120

Query: 121 YRELSEMMRQADTDGDGVISFNEFTTVMAKSAADFLGLTFA 162
           YRELS+MMRQADTDGDGVISFNEFTTVMAKSAADFLGLTFA
Sbjct: 121 YRELSDMMRQADTDGDGVISFNEFTTVMAKSAADFLGLTFA 161

[Close]

BLAST of Lsi04G003100 vs. NCBI nr
Match: XP_038891550.1 (probable calcium-binding protein CML16 [Benincasa hispida])

HSP 1 Score: 308.5 bits (789), Expect = 3.3e-80
Identity = 157/161 (97.52%), Postives = 160/161 (99.38%), Query Frame = 0


Query: 1   MASIQSDQQLKQLHDIFRRFDMNSDGSLTQLELGALLRSLGIKPSGDQLHSLLSNMDSNG 60
           MAS+QSDQQ+KQLHDIFRRFDMNSDGSLTQLELGALLRSLGIKPSGDQLHSLLSNMDSNG
Sbjct: 1   MASLQSDQQVKQLHDIFRRFDMNSDGSLTQLELGALLRSLGIKPSGDQLHSLLSNMDSNG 60

Query: 61  NGSIEFDELVNAILPDMNDDILVNQEQLLEVFRSFDRDGNGYITAAELAGSMAKMGHPLT 120
           NGSIEFDELVNAILPDMNDDILVNQEQLL+VFRSFDRDGNGYITAAELAGSMAKMGHPLT
Sbjct: 61  NGSIEFDELVNAILPDMNDDILVNQEQLLDVFRSFDRDGNGYITAAELAGSMAKMGHPLT 120

Query: 121 YRELSEMMRQADTDGDGVISFNEFTTVMAKSAADFLGLTFA 162
           Y ELSEMMRQADTDGDGVISFNEFTTVMAKSAADFLGLTFA
Sbjct: 121 YHELSEMMRQADTDGDGVISFNEFTTVMAKSAADFLGLTFA 161

[Close]

BLAST of Lsi04G003100 vs. NCBI nr
Match: KAG6601337.1 (putative calcium-binding protein CML16, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 296.2 bits (757), Expect = 1.7e-76
Identity = 151/161 (93.79%), Postives = 160/161 (99.38%), Query Frame = 0


Query: 1   MASIQSDQQLKQLHDIFRRFDMNSDGSLTQLELGALLRSLGIKPSGDQLHSLLSNMDSNG 60
           MAS+QSD QLKQLHDIFRRFDMNSDGSLTQLELGALLRSLGIKPSGDQLHSLLSNMDSNG
Sbjct: 1   MASLQSD-QLKQLHDIFRRFDMNSDGSLTQLELGALLRSLGIKPSGDQLHSLLSNMDSNG 60

Query: 61  NGSIEFDELVNAILPDMNDDILVNQEQLLEVFRSFDRDGNGYITAAELAGSMAKMGHPLT 120
           NGSIEFDELVNAILPD+ND+ILVNQEQL+EVF+SFDRDGNG+ITA+ELAGSMAKMGHPL+
Sbjct: 61  NGSIEFDELVNAILPDLNDNILVNQEQLMEVFKSFDRDGNGFITASELAGSMAKMGHPLS 120

Query: 121 YRELSEMMRQADTDGDGVISFNEFTTVMAKSAADFLGLTFA 162
           YRELSEMMRQADTDGDGVISFNEFTTVMA+SAADFLGLTFA
Sbjct: 121 YRELSEMMRQADTDGDGVISFNEFTTVMARSAADFLGLTFA 160

[Close]

BLAST of Lsi04G003100 vs. NCBI nr
Match: XP_022987337.1 (probable calcium-binding protein CML16 [Cucurbita maxima])

HSP 1 Score: 295.8 bits (756), Expect = 2.2e-76
Identity = 151/161 (93.79%), Postives = 160/161 (99.38%), Query Frame = 0


Query: 1   MASIQSDQQLKQLHDIFRRFDMNSDGSLTQLELGALLRSLGIKPSGDQLHSLLSNMDSNG 60
           MAS+QSD QLKQLHDIFRRFDMNSDGSLTQLELGALLRSLGIKPSGDQLHSLLSNMDSNG
Sbjct: 1   MASMQSD-QLKQLHDIFRRFDMNSDGSLTQLELGALLRSLGIKPSGDQLHSLLSNMDSNG 60

Query: 61  NGSIEFDELVNAILPDMNDDILVNQEQLLEVFRSFDRDGNGYITAAELAGSMAKMGHPLT 120
           NGSIEFDELVNAILPD+ND+ILVNQEQL+EVF+SFDRDGNG+ITA+ELAGSMAKMGHPL+
Sbjct: 61  NGSIEFDELVNAILPDLNDNILVNQEQLMEVFKSFDRDGNGFITASELAGSMAKMGHPLS 120

Query: 121 YRELSEMMRQADTDGDGVISFNEFTTVMAKSAADFLGLTFA 162
           YRELSEMMRQADTDGDGVISFNEFTTVMA+SAADFLGLTFA
Sbjct: 121 YRELSEMMRQADTDGDGVISFNEFTTVMARSAADFLGLTFA 160

[Close]

BLAST of Lsi04G003100 vs. TAIR 10
Match: AT3G25600.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 246.1 bits (627), Expect = 1.9e-65
Identity = 123/159 (77.36%), Postives = 142/159 (89.31%), Query Frame = 0


Query: 1   MASIQSDQQLKQLHDIFRRFDMNSDGSLTQLELGALLRSLGIKPSGDQLHSLLSNMDSNG 60
           MAS +   Q+KQL DIF RFDM+ DGSLTQLEL ALLRSLGIKP GDQ+  LL+ +D NG
Sbjct: 1   MASTKPTDQIKQLKDIFARFDMDKDGSLTQLELAALLRSLGIKPRGDQISLLLNQIDRNG 60

Query: 61  NGSIEFDELVNAILPDMNDDILVNQEQLLEVFRSFDRDGNGYITAAELAGSMAKMGHPLT 120
           NGS+EFDELV AILPD+N+++L+NQEQL+EVFRSFDRDGNG ITAAELAGSMAKMGHPLT
Sbjct: 61  NGSVEFDELVVAILPDINEEVLINQEQLMEVFRSFDRDGNGSITAAELAGSMAKMGHPLT 120

Query: 121 YRELSEMMRQADTDGDGVISFNEFTTVMAKSAADFLGLT 160
           YREL+EMM +AD++GDGVISFNEF+ +MAKSAADFLGLT
Sbjct: 121 YRELTEMMTEADSNGDGVISFNEFSHIMAKSAADFLGLT 159

[Close]

BLAST of Lsi04G003100 vs. TAIR 10
Match: AT1G18530.1 (EF hand calcium-binding protein family )

HSP 1 Score: 230.3 bits (586), Expect = 1.1e-60
Identity = 108/152 (71.05%), Postives = 137/152 (90.13%), Query Frame = 0


Query: 7   DQQLKQLHDIFRRFDMNSDGSLTQLELGALLRSLGIKPSGDQLHSLLSNMDSNGNGSIEF 66
           + Q++QL DIF RFDM++DGSLT LEL ALLRSLG+KPSGDQ+H LL++MDSNGNG +EF
Sbjct: 2   EDQIRQLKDIFDRFDMDADGSLTILELAALLRSLGLKPSGDQIHVLLASMDSNGNGFVEF 61

Query: 67  DELVNAILPDMNDDILVNQEQLLEVFRSFDRDGNGYITAAELAGSMAKMGHPLTYRELSE 126
           DELV  ILPD+N+++L+N EQLLE+F+SFDRDGNG+I+AAELAG+MAKMG PLTY+EL+E
Sbjct: 62  DELVGTILPDLNEEVLINSEQLLEIFKSFDRDGNGFISAAELAGAMAKMGQPLTYKELTE 121

Query: 127 MMRQADTDGDGVISFNEFTTVMAKSAADFLGL 159
           M+++ADT+GDGVISF EF ++MAKSA D+ GL
Sbjct: 122 MIKEADTNGDGVISFGEFASIMAKSAVDYFGL 153

[Close]

BLAST of Lsi04G003100 vs. TAIR 10
Match: AT3G03000.1 (EF hand calcium-binding protein family )

HSP 1 Score: 163.3 bits (412), Expect = 1.6e-40
Identity = 84/148 (56.76%), Postives = 104/148 (70.27%), Query Frame = 0


Query: 7   DQQLKQLHDIFRRFDMNSDGSLTQLELGALLRSLGIKPSGDQLHSLLSNMDSNGNGSIEF 66
           D+QL +L +IFR FD N DGSLT+LELG+LLRSLG+KPS DQL +L+   D N NG +EF
Sbjct: 15  DEQLAELREIFRSFDQNKDGSLTELELGSLLRSLGLKPSQDQLDTLIQKADRNNNGLVEF 74

Query: 67  DELVNAILPDMNDDILVNQEQLLEVFRSFDRDGNGYITAAELAGSMAKMGHPLTYRELSE 126
            E V  + PD+        +QL  +FR FDRDGNGYITAAELA SMAK+GH LT  EL+ 
Sbjct: 75  SEFVALVEPDL-VKCPYTDDQLKAIFRMFDRDGNGYITAAELAHSMAKLGHALTAEELTG 134

Query: 127 MMRQADTDGDGVISFNEFTTVMAKSAAD 155
           M+++AD DGDG I F EF   +  +A D
Sbjct: 135 MIKEADRDGDGCIDFQEFVQAITSAAFD 161

[Close]

BLAST of Lsi04G003100 vs. TAIR 10
Match: AT1G32250.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 155.6 bits (392), Expect = 3.4e-38
Identity = 79/152 (51.97%), Postives = 106/152 (69.74%), Query Frame = 0


Query: 7   DQQLKQLHDIFRRFDMNSDGSLTQLELGALLRSLGIKPSGDQLHSLLSNMDSNGNGSIEF 66
           ++Q+ +L +IFR FD N DGSLTQLELG+LLR+LG+KPS DQ  +L+   D+  NG +EF
Sbjct: 11  EEQINELREIFRSFDRNKDGSLTQLELGSLLRALGVKPSPDQFETLIDKADTKSNGLVEF 70

Query: 67  DELVNAILPDM----NDDILVNQEQLLEVFRSFDRDGNGYITAAELAGSMAKMGHPLTYR 126
            E V  + P++           +EQLL +FR FD DGNG+ITAAELA SMAK+GH LT  
Sbjct: 71  PEFVALVSPELLSPAKRTTPYTEEQLLRLFRIFDTDGNGFITAAELAHSMAKLGHALTVA 130

Query: 127 ELSEMMRQADTDGDGVISFNEFTTVMAKSAAD 155
           EL+ M+++AD+DGDG I+F EF   +  +A D
Sbjct: 131 ELTGMIKEADSDGDGRINFQEFAKAINSAAFD 162

[Close]

BLAST of Lsi04G003100 vs. TAIR 10
Match: AT1G66410.1 (calmodulin 4 )

HSP 1 Score: 125.6 bits (314), Expect = 3.7e-29
Identity = 62/148 (41.89%), Postives = 96/148 (64.86%), Query Frame = 0


Query: 1   MASIQSDQQLKQLHDIFRRFDMNSDGSLTQLELGALLRSLGIKPSGDQLHSLLSNMDSNG 60
           MA   +D+Q+ +  + F  FD + DG +T  ELG ++RSLG  P+  +L  +++ +D++G
Sbjct: 1   MADQLTDEQISEFKEAFSLFDKDGDGCITTKELGTVMRSLGQNPTEAELQDMINEVDADG 60

Query: 61  NGSIEFDELVNAILPDMNDDILVNQEQLLEVFRSFDRDGNGYITAAELAGSMAKMGHPLT 120
           NG+I+F E +N +   M D    ++E+L E FR FD+D NG+I+AAEL   M  +G  LT
Sbjct: 61  NGTIDFPEFLNLMAKKMKD--TDSEEELKEAFRVFDKDQNGFISAAELRHVMTNLGEKLT 120

Query: 121 YRELSEMMRQADTDGDGVISFNEFTTVM 149
             E+ EM+R+AD DGDG I++ EF  +M
Sbjct: 121 DEEVEEMIREADVDGDGQINYEEFVKIM 146

The following BLAST results are available for this feature:

BLAST of Lsi04G003100 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LI84	2.7e-64	77.36	Probable calcium-binding protein CML16 OS=Arabidopsis thaliana OX=3702 GN=CML16 ...	[more]
Q9FZ75	1.5e-59	71.05	Probable calcium-binding protein CML15 OS=Arabidopsis thaliana OX=3702 GN=CML15 ...	[more]
Q6L5F4	1.5e-54	70.86	Probable calcium-binding protein CML14 OS=Oryza sativa subsp. japonica OX=39947 ...	[more]
Q5ZD81	1.8e-49	61.54	Probable calcium-binding protein CML12 OS=Oryza sativa subsp. japonica OX=39947 ...	[more]
Q9AWK2	3.5e-40	57.05	Probable calcium-binding protein CML11 OS=Oryza sativa subsp. japonica OX=39947 ...	[more]

BLAST of Lsi04G003100 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S3BF88	3.2e-81	98.76	probable calcium-binding protein CML16 OS=Cucumis melo OX=3656 GN=LOC103489229 P...	[more]
A0A0A0KUE0	7.2e-81	98.14	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G608150 PE=4 SV=1	[more]
A0A6J1JA36	1.1e-76	93.79	probable calcium-binding protein CML16 OS=Cucurbita maxima OX=3661 GN=LOC1114848...	[more]
A0A6J1GX12	2.4e-76	93.17	probable calcium-binding protein CML16 OS=Cucurbita moschata OX=3662 GN=LOC11145...	[more]
A0A6J1D725	1.6e-75	95.03	probable calcium-binding protein CML16 OS=Momordica charantia OX=3673 GN=LOC1110...	[more]

BLAST of Lsi04G003100 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_008446524.1	6.7e-81	98.76	PREDICTED: probable calcium-binding protein CML16 [Cucumis melo]	[more]
XP_004135127.1	1.5e-80	98.14	probable calcium-binding protein CML16 [Cucumis sativus] >KGN52027.1 hypothetica...	[more]
XP_038891550.1	3.3e-80	97.52	probable calcium-binding protein CML16 [Benincasa hispida]	[more]
KAG6601337.1	1.7e-76	93.79	putative calcium-binding protein CML16, partial [Cucurbita argyrosperma subsp. s...	[more]
XP_022987337.1	2.2e-76	93.79	probable calcium-binding protein CML16 [Cucurbita maxima]	[more]

BLAST of Lsi04G003100 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G25600.1	1.9e-65	77.36	Calcium-binding EF-hand family protein	[more]
AT1G18530.1	1.1e-60	71.05	EF hand calcium-binding protein family	[more]
AT3G03000.1	1.6e-40	56.76	EF hand calcium-binding protein family	[more]
AT1G32250.1	3.4e-38	51.97	Calcium-binding EF-hand family protein	[more]
AT1G66410.1	3.7e-29	41.89	calmodulin 4	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002048	EF-hand domain	SMART	SM00054	efh_1	coord: 12..40 e-value: 0.051 score: 22.7 coord: 123..151 e-value: 1.0E-6 score: 38.3 coord: 48..76 e-value: 1.8 score: 15.8 coord: 87..115 e-value: 5.7E-4 score: 29.2
IPR002048	EF-hand domain	PFAM	PF13499	EF-hand_7	coord: 86..148 e-value: 3.3E-14 score: 53.1 coord: 11..72 e-value: 2.9E-12 score: 46.9
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 119..154 score: 13.620752
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 83..118 score: 14.624987
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 44..79 score: 10.356991
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 8..43 score: 13.007053
IPR002048	EF-hand domain	CDD	cd00051	EFh	coord: 87..149 e-value: 4.9385E-18 score: 71.4249
None	No IPR available	GENE3D	1.10.238.10		coord: 105..157 e-value: 1.5E-15 score: 58.8
None	No IPR available	GENE3D	1.10.238.10		coord: 1..104 e-value: 6.3E-29 score: 102.4
None	No IPR available	PANTHER	PTHR23050:SF351	CALCIUM-BINDING PROTEIN CML15-RELATED	coord: 7..159
None	No IPR available	PANTHER	PTHR23050	CALCIUM BINDING PROTEIN	coord: 7..159
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 132..144
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 96..108
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 21..33
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 57..69
IPR011992	EF-hand domain pair	SUPERFAMILY	47473	EF-hand	coord: 5..149

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi04G003100.1	Lsi04G003100.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0050790	regulation of catalytic activity
molecular_function	GO:0032440	2-alkenal reductase [NAD(P)+] activity
molecular_function	GO:0005509	calcium ion binding
molecular_function	GO:0030234	enzyme regulator activity