Lsi04G001740 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi04G001740
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description3-oxo-5-alpha-steroid 4-dehydrogenase 1-like
Locationchr04: 1893791 .. 1901091 (+)
RNA-Seq ExpressionLsi04G001740
SyntenyLsi04G001740
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AAATATTTACACAGAGGATTTCATGGATACTTTATCGGAGTTTGTTTTTCCGCCGCCGTCTTCGCTTTTTGTGAACGTGATGACGGTGGTGAGCTCCGCGGTGGTGGCGGTGGTAGGGCTATCGGAAGCGAGGGGGAAGCATATGAAGTATTCCAAGTTCTGGAATACTACGAATAATGCTCCGAATTCTTCTAATTTATCGGCTAAGTTAGGAATGCTATTGGCTTATACGCCGGCGTTTCTTGTCGGCATTGTATCGTTGTGGTTGTTTCCGAACGAGGATCGGAGGATTTTTCTGCTCAAGTCTGCTCTCATCTTGCATTTCTTGAAGAGAAACCTTGAGGTAATCTCTCTAGATCTGTCTCTGTTTTTCTCTGATCCTGGAAACAGATAATGTAGATTGAATCGATTATCTTAATTTTCTGGCGTTATTTCGTGATTTACTGATTCGCAGTTCAATTTCTCAAGAGGAATTTGTTGTTCATGTTTGTTTGATCTAATCCTGGAAAAATGTTGAAGAATATGCAATTTCTTCTCCATTTGTATGATTATTAACTATTCCTAATTGTATATGATCTCGATTCTTTGGATTGGAGCAAGAATCAGGGGCTCTTCTTCGAGAACCTTCTTATTTTTCTCAAGTTTTTAAAAACCTGATGAAAATTCCCGCGCAGAGAAATGGATGGAGATTTTTAAATCTTCAAAACGCATTTTCACTATGCGAGTTCAATTATGCTTGCTGGTTTTGTAGAATCTTTTTAGAACTATTTAATATATATTGTTGATTATCACCTACTACTAGTTTAGGGTCGATTCTTAAAAAATCTTTTTCTTATCCCTTAACGTTTTACTATAAATTTTAAAAACATATTCACATATTGTGCACGATAAGAAAAATTTGTAATCGTGCCAAGAGACCAGAACTAGCTTCCACATAGTTTTCATGATTTTTTTTCTAAAAACCAGTCTACCATTATATGTCGTCTAGGTTTTATTTTCTTATCAGATTTTTCATCGTCTTTGTCGCTTTAGTTTTCTAAGAATTTGTGTTCTTTAATTATTTTGGGTTTATTTATAAATTCAGGAGTATCCGATTAGTGATTTTCAACAATTTTGAATTTATACAAATTATTTAATTTATAAAGTTTGTTTGATTTTTAATTAAAATTTATCATGAAAATTATAATTATTATTTAATTAAACTAATTTCAATAAAAATTAACATTGAGTGGCTAAATTTAAGATTTATGGAAAGTATGAGATCTAAAAAAAAATCATTTTAAGCATTTATGTATTGAAACAAATCTTAAATTGACATTATTGAACTTTTCTTTTGAAAATTGTACACACAGGTAGTGTTTCTTCACAAATACAGCAGCAAAATGGTAATTGATACAGTAATTACAATCTCCTTCAGCTACTTCTCCTCCACAGCAATCATGATATACACTCAGCATCTTTCACAAGGCCTTCCACAACCTTCCATTGATCTCAAAAACATTGGTATTGCTCTGTTTTCAATAGGCATTATTGGGAATTTTTACCATCATTATCTCTTGTCCCAAGCTAGAAAACAGGGAGAAACAAGCTATAAAATCCCCAAAGGAGGCCTCTTCAGCCTTGTCATTTGCCCTCACTATATGTTTGAAACAATTGAGTATTTCGGGTTTGCTTTAATCTCTCAAACAATCTATTCATTCTTCTTTGCCTTTGCCACTGCTTTGTATTTGAGTGGAAGGAGCTATGCCACTAGGAAATGGTATGTCTCCAAATTTGAAGACTTTCCTACCCATCTTAAAGCCCTTATCCCCTTTCTATTTTAGCCCTTTTTCAATTGTTAATTAATTATTTGTCTTTTTTTTTTTAGTTTGAAAACATTTCACATTGATGAGCTCTGTTCAAGTTGGTTGTTTAAATTTTTTTTTTTTTTTTTTTTTTTATTGTTTTCTGTGACAAATTTGACGATAAAATTATGGTATTGATGTTGATGTTCTTTTTTCTTTCGACAATATGCGGATGAAAATTTATACGTTTAATGGTGTAGGGGTAAATATTTGGACAAATATTAATTTATACCTTTCAATTTTGGTGGTTTGTATCAATTTAAATCCTAAACTAATAATTGTATTCAATTAGGACTTAAACTTTGGAGGTTTTATCAATTTAAATTCTGAACTTTTTGTATCAATTAAAACCTTGAACTTCCATAAGTATATCAATTTAAACTTTCAATTTTTATAAATATATCCATTTAAACAATTAATTTTCTTAAGTGTATTTAAATAAAAGATAATGGAGGGGTTCAATTTTTGTAGTTGTCGATTGTCGTCTATTGAAAGAGTGACTTTGCCTCAAAACTACGATATTTTTCTAATGAATTTTGAGACATATATGTTTATGATAAATTTTAAATTTGCAACTAAAAAAGAATCAAATAATACAATGGAAATGGTATTAATGAAATAGAATTATCCGTTCCCTAAAAATATAAATTAATTCTCTTCTTATGAATACATCTTGTGAGAGAGTCATGTAAGCTATAATTTTTTCAACGTTTAAATTTCATATTTTTTCAAATGTACCGACTTAATCTTTCTAAATATATGATTACTCTTGTCCAAAAAAAATGTTTATTATATATATATATTTTTAAAAAATATTACTATAATAAAAAAAATTATAAAAAAAAATACCATGTACCAAATGCGTGAGATTTAAGTACACGACAATCATCAATTTCAGGCAATTATTAGTTATTTTCCTTTCACTTTTTTTTTTTTTTGAAACATTACCAATACGACTTTACATCCAATCTGAAAACCAAAACAAAATCCAAGAGAAAAAGATAATAAATGATACCATAATTAACCGTTTATATTAAATTCTATATTTTTCTTGAAAATTTTAAATTACATATTTATCTTATATATGATAAATTAAAATATCATTTTAGTCTCTAAATTTTGAAGTTGGTTTAATTTTATATATTCAATTATCTAATTTTAATAATTGTATTTTCAATAAATTTTAAATTTAGTATTTAAACTTTAAATTTTACCAAAATTAGATAAATAATAACAATAATTTTCATACAAAAAAGTACAATATGAGAATATCTTTTAAAAATTTATAGTAAAAAAGCTAATAAACAACAAATTAACTATAAACTAGTAGCAAGGACTAATATTAAGATTCGTCGAATATTAGAGACTAAAATTAGATAGTTGAAATTATAGAAACTAGAAGGACTAAAATGGTATTTTAGAAAATAAAATATTATAGATTATTTTTTTAAAAAAAATATAGTAGGTTATTAGTAAAAGTATATATGACATGCATGGGTGTAAAAAATAACCATTTTTAAAAGACATACTTTTAGTCTTCCATTAGCACATGAAAAACATGTTACCTTCAAACTTACTTTGTTTTTTCTTTTTTTTTTCTATCACTAAATTATTGTTTTTTCTGACACATTAATTAACATATTTAAATGTTACTTGATTCAAGTCACTCACTAATAAAATTAAACCAACTCCTAAAAAATTATTTCCATGTAATTTGGCATTGCTTAAGGATATGTCCACCAAATATATTAACTCAAAACAACAATTTAGTGCCACGTGGTACTTCTTTATTTGTAAATAATACTAATTTAAACTTCAACTTTCTTAATTAAAAGAAAAAACACAAAATATATATTAAACTTAATTATTAGCATTTTTGGGTTAGTATCAATTTAAATTATGAACTTTAATAAGTGTATCAATTTGAACATTCCATTATATTTTATTCGAATAAACATAACGCGAGGCTTATAAAATCAATTTAGAATCTCAATTAAGATTTTTTAAAAAATTATCCATGCAAATTCTAATTTTCCATTTTGTTAAATCGACATTTGAAGAAGTCTACCTACGTGTAAGAATTAATGCATTAGTTTTCAAATATACTACTTAATAAAGATTCTAAATCATCAATCCACTTATAACAATTTAGAAATTTAATTGAAAAAATTAAATGTCTACATAATTTTTTTTTTTCAAATTAAATTTGAATGAATGTGTAAATTGATATATTTATGAAAGTTCATAGTTTTAAGTGACACAATTATTAGTTTATAGTTTAAATTGATATTTCCAAACAATTTAACGGTGTAAATTGAATTTTTTCATAATAATTATTATTATAATCATTTTTCTTGTTGTTTTGTTACTTGCTTTAGTTAGCCTGTTATACTTTCTTTGCTGGCTATATAACTACCAAGTGTCTAATTAATAAAATGGGTAAAGTATTATATATATAATTTTGTATATAGAAGATATTATATAATGATTTTTAAAAGGACACATACATTTTACCAAAATACCCCTTGTGTTTTCACCGGGATCCATTCTATAAAACGTCAGTCTCTCTTTCTCAGTGCTTAAAAAACGAGACTTACAGGAGAAATTTACTCAAGAAGGCTTCACCGATCCAAGTCAGGACGGCAATGAATACTTTATCGGCGTTTCTTTTTCCGCCGCCGCCATCACTGTTCGTTAACGTGATGACGGTGGTGAGTTCGGCGGTGGTAGCGGTGATAGGGCTTTCGGAAGCGAGAGGAACGCATTTACAGTATTCAAAGTTCTGGAATACGAGTAATAGTGCTACGAAATCATCTAACTCAGAACAACTAAGGATTTCGTCAAGATTGGGGATGTTCCTGCTTTATACTCCGGCGGCTCTCGCCGGCGCTGTGTCGTTCTGGTTCTTTCCGAACGATGATTGGAGGATTCTTCTGCTTAAATCCGCTCTCACTCTCCATTTCTTCAAGCGGGACTTTGAGGTACTCTGTTTCTCCCTAATTTTGTTCAATCTAAACCTAGAAATAGATTTATGTTGTAGAATGAAAATTTCTCGCTCTGTGTTTGTGATTAATACGTAAAGATTCGTTTTCTTATGACGAATTAGTTATTCAATATTGTTTGATTCCTTTTCGGTAATCGATCAAACGAGAATCGGGGATTTGTTGAGGTCGTGTTGATCTTCCTCTGTACTTTAGATTTGATTTGATTTTGATTGATTCGTATTCTCGCGAGTCCTTATTTATTCAAAATTTTAAACTAGTTTGATCTATTTTTGGAACTTGGACAAGAAACAGGATTTGGTCGACATTCACTTTCTGCATGAAAATTACTTAATTAGTATACCTTTCTCATCACCATTAATAATTTGACAAATATTAGCATTTGATCTCTAGTCTATTTGGTCGACCATCTGGATTCTTAGGTCCGTGATTTTTTTTTTTTTTTTTTTTTTTTTTTGGTGTTTTTTACCTCTATTAAATTCAATAAATATGTGTTGAGATTATAGGTTGAGAAACCATTTTTTAAAATTGCTTTTAGATTCTGTGATCGACAAATTTTTGGAAATAACAATTTTATATTTTCATCCATGTATTTTTTATTTTGAATTTTAAATTTTAAAATACAAAATTATATTAGATAAAGATAAAAAATATTTAGATATTTTACTAATTTATTTATTTTTAATTAAAATATGACGTATTATAAATTATATAATATTAAAAATTAATAATTATACAAAATTTAACTTAAAACATGTTTATTGAATAGTTTGTTTTCATACTATTTACCAAACAAATTTTTAGCTTTCATAACATATCTTTTTGTGCATTTAGTCCTGAGTTTTGAAAAGATATATTTTTTTATTATTTTAGATATTTATATGCATTTTGGACAAGAAAAATAAGTTTTAAAAATAATTTTAGAGAGACACTATTTTTATAAATTCAGATATTAAAATTTAGAGACGGAACATTTTTTTTTTTCTAAGTCTAAAAAGGTAATTCCCTCTATTTTTTTTTTCGTTTCTTTGAAAACAATTCCTCTCTCTCTTCTTTACGTCACTTCTCTTTCTTTTTCTAGTAAACTATTAGTTTTATACTTCTTTCCTTACTATTAGTTTAAGTTGAGGGTCAATCGAGAATTTAATATGCTACTAGAGTCGGTAGTCTAGGAACTCTTATGTTCTTAATTTTTACTTGTTGATTTTTCTACATATTTTAAGTCCACAAGTGAGAAGAATGTTAGATAAATTTATTTTCATTCATCAATTAAAATTTTTAATCAATTAACGATTTAAAAGTTTAAAAGAAGTCACAACATCACAAAACTCTGCTCAATCCAACCCAACTCAATTCGTAAACACACTTAAATTATGAGATGTTTTTGCATGTTCTTTACCTTCTGTATGGAAAAATTCTACAGGTACTGTTTGTTCACAAATACAGCGGCAAAATGGTAGTTGATTCAGCAATTTTAATCTCCCTCAGCTACTTCTCCTCTACGGCACTCATGATATACACTCAACATCTATCAAAATCACTTCCAGAGCCATCCATTGATCTGAAAAACATTGGAATTGCTCTGTTTTTGATAGGCATTACTGGGAACATGTATCACCATTATCTTTTGTCTCAAACTAGAAAAAAAGGAGAAACAGGCTACAAAATTCCCAAAGGAGGCCTCTTCAGCATTGTGATTTGCCCTCATTATCTCTGTGAAATTACTGTGTATTTTGGGTTCGCTCTCATCTCTCAGACACTCTACTCATTGTGCTTCGCCATTGCCACAGCTATCTATTTGGGTGGGAGAAGCTACGCCACTAGGAAATGGTACGTCTCTAAATTTGACGACTTCCCCCACCATACCAAAGCTTTAATTCCCTTCGTTTTTTAGCCCATATAAATTATAATAAATATCCTTCAACTTTGCTCTTTGTTTTAAAAATATACCCTTGTTCCTTTCTAAGTCGTAAGTTTGATCTTTCACTCCTTTAGTTGTTGTACTAAAAAAAATCTAAATTTGAATTACTACACTGTTTTAAGTCAATGCTACGTAATTTCAAGTCTTTATTTTCGTTTGAAATTGTAAAGGATTTTATTCTAAAGGTTGTTTTGGAATGTTTATGAAGAATAAGGATAATATTACCATTTTAAGAAGTTTATCTCATATTTTTTTAAACAAAATCAAAGTTTAAGGGTATTTTTTTAAAAAATAATTTAGTCTTTTTGGCTTTTACACGGTTGGTTTGTTTCTTTTTCTATGTTGTATCATCAACTATATTAAATAATGTAATTTTCATTTATGGAATAATTTTTATTTTTCCATTTATTTGGAATTGTTGTGTTATTTTAGGGGGGTTGTGGGTAATTTCACTTTATGTAATCATTGATTCATTGTACTGTATCCAATGTCTCTCTGGCCATAATCAATAATTTTTTAATTATTATTTTTTTT

mRNA sequence

AAATATTTACACAGAGGATTTCATGGATACTTTATCGGAGTTTGTTTTTCCGCCGCCGTCTTCGCTTTTTGTGAACGTGATGACGGTGGTGAGCTCCGCGGTGGTGGCGGTGGTAGGGCTATCGGAAGCGAGGGGGAAGCATATGAAGTATTCCAAGTTCTGGAATACTACGAATAATGCTCCGAATTCTTCTAATTTATCGGCTAAGTTAGGAATGCTATTGGCTTATACGCCGGCGTTTCTTGTCGGCATTGTATCGTTGTGGTTGTTTCCGAACGAGGATCGGAGGATTTTTCTGCTCAAGTCTGCTCTCATCTTGCATTTCTTGAAGAGAAACCTTGAGGTAGTGTTTCTTCACAAATACAGCAGCAAAATGGTAATTGATACAGTAATTACAATCTCCTTCAGCTACTTCTCCTCCACAGCAATCATGATATACACTCAGCATCTTTCACAAGGCCTTCCACAACCTTCCATTGATCTCAAAAACATTGGTATTGCTCTGTTTTCAATAGGCATTATTGGGAATTTTTACCATCATTATCTCTTGTCCCAAGCTAGAAAACAGGGAGAAACAAGCTATAAAATCCCCAAAGGAGGCCTCTTCAGCCTTGTCATTTGCCCTCACTATATGTTTGAAACAATTGAGTATTTCGGGTTTGCTTTAATCTCTCAAACAATCTATTCATTCTTCTTTGCCTTTGCCACTGCTTTGTATTTGAGTGGAAGGAGCTATGCCACTAGGAAATGGAGAAATTTACTCAAGAAGGCTTCACCGATCCAAGTCAGGACGGCAATGAATACTTTATCGGCGTTTCTTTTTCCGCCGCCGCCATCACTGTTCGTTAACGTGATGACGGTGGTGAGTTCGGCGGTGGTAGCGGTGATAGGGCTTTCGGAAGCGAGAGGAACGCATTTACAGTATTCAAAGTTCTGGAATACGAGTAATAGTGCTACGAAATCATCTAACTCAGAACAACTAAGGATTTCGTCAAGATTGGGGATGTTCCTGCTTTATACTCCGGCGGCTCTCGCCGGCGCTGTGTCGTTCTGGTTCTTTCCGAACGATGATTGGAGGATTCTTCTGCTTAAATCCGCTCTCACTCTCCATTTCTTCAAGCGGGACTTTGAGGTACTGTTTGTTCACAAATACAGCGGCAAAATGGTAGTTGATTCAGCAATTTTAATCTCCCTCAGCTACTTCTCCTCTACGGCACTCATGATATACACTCAACATCTATCAAAATCACTTCCAGAGCCATCCATTGATCTGAAAAACATTGGAATTGCTCTGTTTTTGATAGGCATTACTGGGAACATGTATCACCATTATCTTTTGTCTCAAACTAGAAAAAAAGGAGAAACAGGCTACAAAATTCCCAAAGGAGGCCTCTTCAGCATTGTGATTTGCCCTCATTATCTCTGTGAAATTACTGTGTATTTTGGGTTCGCTCTCATCTCTCAGACACTCTACTCATTGTGCTTCGCCATTGCCACAGCTATCTATTTGGGTGGGAGAAGCTACGCCACTAGGAAATGGTACGTCTCTAAATTTGACGACTTCCCCCACCATACCAAAGCTTTAATTCCCTTCGTTTTTTAGCCCATATAAATTATAATAAATATCCTTCAACTTTGCTCTTTGTTTTAAAAATATACCCTTGTTCCTTTCTAAGTCGTAAGTTTGATCTTTCACTCCTTTAGTTGTTGTACTAAAAAAAATCTAAATTTGAATTACTACACTGTTTTAAGTCAATGCTACGTAATTTCAAGTCTTTATTTTCGTTTGAAATTGTAAAGGATTTTATTCTAAAGGTTGTTTTGGAATGTTTATGAAGAATAAGGATAATATTACCATTTTAAGAAGTTTATCTCATATTTTTTTAAACAAAATCAAAGTTTAAGGGTATTTTTTTAAAAAATAATTTAGTCTTTTTGGCTTTTACACGGTTGGTTTGTTTCTTTTTCTATGTTGTATCATCAACTATATTAAATAATGTAATTTTCATTTATGGAATAATTTTTATTTTTCCATTTATTTGGAATTGTTGTGTTATTTTAGGGGGGTTGTGGGTAATTTCACTTTATGTAATCATTGATTCATTGTACTGTATCCAATGTCTCTCTGGCCATAATCAATAATTTTTTAATTATTATTTTTTTT

Coding sequence (CDS)

ATGGATACTTTATCGGAGTTTGTTTTTCCGCCGCCGTCTTCGCTTTTTGTGAACGTGATGACGGTGGTGAGCTCCGCGGTGGTGGCGGTGGTAGGGCTATCGGAAGCGAGGGGGAAGCATATGAAGTATTCCAAGTTCTGGAATACTACGAATAATGCTCCGAATTCTTCTAATTTATCGGCTAAGTTAGGAATGCTATTGGCTTATACGCCGGCGTTTCTTGTCGGCATTGTATCGTTGTGGTTGTTTCCGAACGAGGATCGGAGGATTTTTCTGCTCAAGTCTGCTCTCATCTTGCATTTCTTGAAGAGAAACCTTGAGGTAGTGTTTCTTCACAAATACAGCAGCAAAATGGTAATTGATACAGTAATTACAATCTCCTTCAGCTACTTCTCCTCCACAGCAATCATGATATACACTCAGCATCTTTCACAAGGCCTTCCACAACCTTCCATTGATCTCAAAAACATTGGTATTGCTCTGTTTTCAATAGGCATTATTGGGAATTTTTACCATCATTATCTCTTGTCCCAAGCTAGAAAACAGGGAGAAACAAGCTATAAAATCCCCAAAGGAGGCCTCTTCAGCCTTGTCATTTGCCCTCACTATATGTTTGAAACAATTGAGTATTTCGGGTTTGCTTTAATCTCTCAAACAATCTATTCATTCTTCTTTGCCTTTGCCACTGCTTTGTATTTGAGTGGAAGGAGCTATGCCACTAGGAAATGGAGAAATTTACTCAAGAAGGCTTCACCGATCCAAGTCAGGACGGCAATGAATACTTTATCGGCGTTTCTTTTTCCGCCGCCGCCATCACTGTTCGTTAACGTGATGACGGTGGTGAGTTCGGCGGTGGTAGCGGTGATAGGGCTTTCGGAAGCGAGAGGAACGCATTTACAGTATTCAAAGTTCTGGAATACGAGTAATAGTGCTACGAAATCATCTAACTCAGAACAACTAAGGATTTCGTCAAGATTGGGGATGTTCCTGCTTTATACTCCGGCGGCTCTCGCCGGCGCTGTGTCGTTCTGGTTCTTTCCGAACGATGATTGGAGGATTCTTCTGCTTAAATCCGCTCTCACTCTCCATTTCTTCAAGCGGGACTTTGAGGTACTGTTTGTTCACAAATACAGCGGCAAAATGGTAGTTGATTCAGCAATTTTAATCTCCCTCAGCTACTTCTCCTCTACGGCACTCATGATATACACTCAACATCTATCAAAATCACTTCCAGAGCCATCCATTGATCTGAAAAACATTGGAATTGCTCTGTTTTTGATAGGCATTACTGGGAACATGTATCACCATTATCTTTTGTCTCAAACTAGAAAAAAAGGAGAAACAGGCTACAAAATTCCCAAAGGAGGCCTCTTCAGCATTGTGATTTGCCCTCATTATCTCTGTGAAATTACTGTGTATTTTGGGTTCGCTCTCATCTCTCAGACACTCTACTCATTGTGCTTCGCCATTGCCACAGCTATCTATTTGGGTGGGAGAAGCTACGCCACTAGGAAATGGTACGTCTCTAAATTTGACGACTTCCCCCACCATACCAAAGCTTTAATTCCCTTCGTTTTTTAG

Protein sequence

MDTLSEFVFPPPSSLFVNVMTVVSSAVVAVVGLSEARGKHMKYSKFWNTTNNAPNSSNLSAKLGMLLAYTPAFLVGIVSLWLFPNEDRRIFLLKSALILHFLKRNLEVVFLHKYSSKMVIDTVITISFSYFSSTAIMIYTQHLSQGLPQPSIDLKNIGIALFSIGIIGNFYHHYLLSQARKQGETSYKIPKGGLFSLVICPHYMFETIEYFGFALISQTIYSFFFAFATALYLSGRSYATRKWRNLLKKASPIQVRTAMNTLSAFLFPPPPSLFVNVMTVVSSAVVAVIGLSEARGTHLQYSKFWNTSNSATKSSNSEQLRISSRLGMFLLYTPAALAGAVSFWFFPNDDWRILLLKSALTLHFFKRDFEVLFVHKYSGKMVVDSAILISLSYFSSTALMIYTQHLSKSLPEPSIDLKNIGIALFLIGITGNMYHHYLLSQTRKKGETGYKIPKGGLFSIVICPHYLCEITVYFGFALISQTLYSLCFAIATAIYLGGRSYATRKWYVSKFDDFPHHTKALIPFVF
Homology
BLAST of Lsi04G001740 vs. ExPASy Swiss-Prot
Match: Q28891 (3-oxo-5-alpha-steroid 4-dehydrogenase 1 OS=Macaca fascicularis OX=9541 GN=SRD5A1 PE=1 SV=1)

HSP 1 Score: 95.5 bits (236), Expect = 1.9e-18
Identity = 45/107 (42.06%), Postives = 66/107 (61.68%), Query Frame = 0

Query: 420 IGIALFLIGITGNMYHHYLLSQTRKKGETGYKIPKGGLFSIVICPHYLCEITVYFGFALI 479
           IG  L+L G+  N++  ++L   RK G+TGYKIP+GGLF  V   +Y  EI  + G+AL 
Sbjct: 157 IGFGLWLAGMLINIHSDHILRNLRKPGDTGYKIPRGGLFEYVTAANYFGEIMEWCGYALA 216

Query: 480 SQTLYSLCFAIATAIYLGGRSYATRKWYVSKFDDFPHHTKALIPFVF 527
           S ++    FA  T  +L GR+    +WY+ KF+++P   K LIPF+F
Sbjct: 217 SWSVQGAAFAFFTFCFLSGRAKEHHRWYLQKFEEYPKFRKILIPFLF 263

BLAST of Lsi04G001740 vs. ExPASy Swiss-Prot
Match: Q28892 (3-oxo-5-alpha-steroid 4-dehydrogenase 2 OS=Macaca fascicularis OX=9541 GN=SRD5A2 PE=1 SV=1)

HSP 1 Score: 95.5 bits (236), Expect = 1.9e-18
Identity = 45/107 (42.06%), Postives = 72/107 (67.29%), Query Frame = 0

Query: 420 IGIALFLIGITGNMYHHYLLSQTRKKGETGYKIPKGGLFSIVICPHYLCEITVYFGFALI 479
           +G+ LF++G+  N++  Y+L Q RK GE  Y+IPKGGLF+ V   ++L EI  + G+AL 
Sbjct: 148 LGVFLFILGMGVNIHSDYILRQLRKPGEITYRIPKGGLFTYVSGANFLGEIIEWIGYALA 207

Query: 480 SQTLYSLCFAIATAIYLGGRSYATRKWYVSKFDDFPHHTKALIPFVF 527
           + +L +L FA  +  +LG R++   ++Y+  F+D+P   KALIPF+F
Sbjct: 208 TWSLPALAFAFFSVCFLGLRAFHHHRFYLKMFEDYPKSRKALIPFIF 254

BLAST of Lsi04G001740 vs. ExPASy Swiss-Prot
Match: O18765 (3-oxo-5-alpha-steroid 4-dehydrogenase 2 OS=Sus scrofa OX=9823 GN=SRD5A2 PE=2 SV=2)

HSP 1 Score: 95.5 bits (236), Expect = 1.9e-18
Identity = 46/108 (42.59%), Postives = 73/108 (67.59%), Query Frame = 0

Query: 419 NIGIALFLIGITGNMYHHYLLSQTRKKGETGYKIPKGGLFSIVICPHYLCEITVYFGFAL 478
           ++G+ LF++G+  N++  Y+L Q RK GE  YKIP+GGLF+ V   ++L EI  + G+AL
Sbjct: 147 SLGVFLFILGMGINIHSDYILRQLRKPGEVIYKIPQGGLFTYVSGANFLGEIIEWIGYAL 206

Query: 479 ISQTLYSLCFAIATAIYLGGRSYATRKWYVSKFDDFPHHTKALIPFVF 527
            + +L +L FA  +  +LG R++   ++YV  F+D+P   KALIPF+F
Sbjct: 207 ATWSLPALAFAFFSLCFLGLRAFHHHRFYVKMFEDYPKSRKALIPFIF 254

BLAST of Lsi04G001740 vs. ExPASy Swiss-Prot
Match: P31213 (3-oxo-5-alpha-steroid 4-dehydrogenase 2 OS=Homo sapiens OX=9606 GN=SRD5A2 PE=1 SV=1)

HSP 1 Score: 94.4 bits (233), Expect = 4.2e-18
Identity = 44/108 (40.74%), Postives = 73/108 (67.59%), Query Frame = 0

Query: 419 NIGIALFLIGITGNMYHHYLLSQTRKKGETGYKIPKGGLFSIVICPHYLCEITVYFGFAL 478
           ++G+ LF++G+  N++  Y+L Q RK GE  Y+IP+GGLF+ V   ++L EI  + G+AL
Sbjct: 147 SLGVFLFILGMGINIHSDYILRQLRKPGEISYRIPQGGLFTYVSGANFLGEIIEWIGYAL 206

Query: 479 ISQTLYSLCFAIATAIYLGGRSYATRKWYVSKFDDFPHHTKALIPFVF 527
            + +L +L FA  +  +LG R++   ++Y+  F+D+P   KALIPF+F
Sbjct: 207 ATWSLPALAFAFFSLCFLGLRAFHHHRFYLKMFEDYPKSRKALIPFIF 254

BLAST of Lsi04G001740 vs. ExPASy Swiss-Prot
Match: P18405 (3-oxo-5-alpha-steroid 4-dehydrogenase 1 OS=Homo sapiens OX=9606 GN=SRD5A1 PE=1 SV=1)

HSP 1 Score: 94.0 bits (232), Expect = 5.5e-18
Identity = 44/107 (41.12%), Postives = 66/107 (61.68%), Query Frame = 0

Query: 420 IGIALFLIGITGNMYHHYLLSQTRKKGETGYKIPKGGLFSIVICPHYLCEITVYFGFALI 479
           IG  L+L G+  N++  ++L   RK G+TGYKIP+GGLF  V   +Y  EI  + G+AL 
Sbjct: 153 IGFGLWLTGMLINIHSDHILRNLRKPGDTGYKIPRGGLFEYVTAANYFGEIMEWCGYALA 212

Query: 480 SQTLYSLCFAIATAIYLGGRSYATRKWYVSKFDDFPHHTKALIPFVF 527
           S ++    FA  T  +L GR+    +WY+ KF+++P   K +IPF+F
Sbjct: 213 SWSVQGAAFAFFTFCFLSGRAKEHHEWYLRKFEEYPKFRKIIIPFLF 259

BLAST of Lsi04G001740 vs. ExPASy TrEMBL
Match: A0A4D6M1Q7 (Enoyl reductase OS=Vigna unguiculata OX=3917 GN=DEO72_LG5g2622 PE=3 SV=1)

HSP 1 Score: 594.0 bits (1530), Expect = 6.3e-166
Identity = 296/526 (56.27%), Postives = 376/526 (71.48%), Query Frame = 0

Query: 1   MDTLSEFVFPPPSSLFVNVMTVVSSAVVAVVGLSEARGKHMKYSKFWNTTNNAPNSSNLS 60
           M T   F+F PP SL V+ ++VVS   +A  GLSE RGKH+ YSKFWN   ++     LS
Sbjct: 3   MSTFLSFIFAPPPSLVVSTLSVVSLVSLANAGLSEIRGKHLNYSKFWNANPSSEKQIKLS 62

Query: 61  AKLGMLLAYTPAFLVGIVSLWLFPNEDRRIFLLKSALILHFLKRNLEVVFLHKYSSKMVI 120
           +K GMLL YTPAFL  + S W+FP+E  R  LL+SA+ LHF KR+ EV+F+HKYS  M++
Sbjct: 63  SKTGMLLLYTPAFLAALASFWIFPHEGLRSTLLQSAVTLHFFKRDFEVLFIHKYSGGMLL 122

Query: 121 DTVITISFSYFSSTAIMIYTQHLSQGLPQPSIDLKNIGIALFSIGIIGNFYHHYLLSQAR 180
           D+ I I+ SYF S A M+Y QHL+QGLP+P I+L   GI +F +GIIGNFYHHYLLS  R
Sbjct: 123 DSAIPITLSYFLSAATMVYAQHLTQGLPEPPINLLYPGIVMFVVGIIGNFYHHYLLSNLR 182

Query: 181 KQGETSYKIPKGGLFSLVICPHYMFETIEYFGFALISQTIYSFFFAFATALYLSGRSYAT 240
            +GE  YKIPKGGLF +VICPHY+FE I+++G + ISQT+Y+F F   T  YL GRSY+T
Sbjct: 183 AKGEKEYKIPKGGLFGIVICPHYLFEIIQFYGISFISQTLYAFCFTLGTTFYLLGRSYST 242

Query: 241 RKWRNLLKKASPIQVRTAMNTLSAFLFPPPPSLFVNVMTVVSSAVVAVIGLSEARGTHLQ 300
           RKW    ++         M+ L +F+FPPPPS+ V+ +TV+S  ++    LSE +G HL 
Sbjct: 243 RKWSE--ERVELAYTAIVMSVLLSFMFPPPPSVVVSGLTVISFVILVSSFLSEIKGKHLN 302

Query: 301 YSKFWNTSNSATKSSNSEQLRISSRLGMFLLYTPAALAGAVSFWFFPNDDWRILLLKSAL 360
           YSKFWN + SA K     Q+++ S++GM LLYTPA LAG  SFW FP    R ++L+SA+
Sbjct: 303 YSKFWNANPSAQK-----QIKLPSKVGMLLLYTPAFLAGLASFWIFPPQSLRSIILQSAV 362

Query: 361 TLHFFKRDFEVLFVHKYSGKMVVDSAILISLSYFSSTALMIYTQHLSKSLPEPSIDLKNI 420
           T HFFKR FEVLF+HKYSG M ++SAI I+LSYF S A M Y+QHL+  LPEP ++L   
Sbjct: 363 TFHFFKRVFEVLFIHKYSGGMTLESAIPITLSYFLSAATMTYSQHLTNGLPEPPMNLLYP 422

Query: 421 GIALFLIGITGNMYHHYLLSQTRKKGETGYKIPKGGLFSIVICPHYLCEITVYFGFALIS 480
           G  +F++ I  N YHHYLLS  RK+GE  YKIPKGGLF +VICPHYL EI V++G   IS
Sbjct: 423 GTVIFVVAIICNFYHHYLLSNLRKEGEKEYKIPKGGLFGLVICPHYLFEIIVFYGIFFIS 482

Query: 481 QTLYSLCFAIATAIYLGGRSYATRKWYVSKFDDFPHHTKALIPFVF 527
           QTLY+  FAI T +YL GRSY+TRKWYVSKF+DFP + KA+IPFVF
Sbjct: 483 QTLYAFSFAIGTNLYLLGRSYSTRKWYVSKFEDFPQNVKAVIPFVF 521

BLAST of Lsi04G001740 vs. ExPASy TrEMBL
Match: A0A103Y9N2 (3-oxo-5-alpha-steroid 4-dehydrogenase, C-terminal OS=Cynara cardunculus var. scolymus OX=59895 GN=Ccrd_016565 PE=3 SV=1)

HSP 1 Score: 527.7 bits (1358), Expect = 5.5e-146
Identity = 285/551 (51.72%), Postives = 359/551 (65.15%), Query Frame = 0

Query: 4   LSEFVFPPPSSLFVNVMTVVSSAVVAVVGLSEARGKHMKYSKFWNTTNNAPNSS-NLSAK 63
           L  F+FP PSS FV  M VV+ + +      E +GKH++YSKF N    A      LS++
Sbjct: 7   LLNFLFPSPSSWFVTAMLVVNLSALTDAAYMEIKGKHLRYSKFLNAGGVAKKQDMKLSSR 66

Query: 64  LGMLLAYTPAFLVGIVSLWLFPNEDRRIFLLKSALILHFLKRNLEVVFLHKYSSKMVIDT 123
            GMLL YTPAFLVG+ S ++ P +D R  LL SAL LHF KR LEV+FLHKYS  M +++
Sbjct: 67  NGMLLFYTPAFLVGVASFFVVPYQDLRFVLLASALTLHFFKRVLEVLFLHKYSGSMALES 126

Query: 124 VITISFSYFSSTAIMIYTQHLSQGLPQPSIDLKNIGIALFSIGIIGNFYHHYLLSQARKQ 183
            I I+ SY SSTA MIY Q LS+ +P+P++DLK +GI LF IG IGNFYHH LL+  R +
Sbjct: 127 PIIITMSYTSSTATMIYAQFLSRDVPEPTVDLKYVGIGLFFIGTIGNFYHHCLLANLRNK 186

Query: 184 GETSYKIPKGGLFSLVICPHYMFETIEYFGFALISQTIYSFFFAFATALYLSGRSYATRK 243
           G+  YKIP+GGLF LVICPHY+FE + Y G + ISQT Y+F F   T  YL GRSY TR 
Sbjct: 187 GDKEYKIPQGGLFGLVICPHYLFEILAYAGISSISQTPYAFAFTLGTIFYLIGRSYGTRG 246

Query: 244 W-----------------------RNLLKKASPIQVRTAMNTLSAF---LFPPPPSLFVN 303
           W                        NL +  + +++   M   S F   LFPPP SLFV 
Sbjct: 247 WYLSKFGDKFPKDVKALIPYCEFANNLRRTPNTLRLIIKMAIPSIFLNLLFPPPSSLFVT 306

Query: 304 VMTVVSSAVVAVIGLSEARGTHLQYSKFWNTSNSATKSSNSEQLRISSRLGMFLLYTPAA 363
            MTVV  + +   G  E +G H+QYSKF N      +    +++++SS+ GM L Y P+ 
Sbjct: 307 TMTVVGFSSLTNAGYKEIKGQHMQYSKFLNV--GVVEKEKEKEMKLSSKNGMLLFYAPSF 366

Query: 364 LAGAVSFWFFPNDDWRILLLKSALTLHFFKRDFEVLFVHKYSGKMVVDSAILISLSYFSS 423
           L G  SF+ FP++D R  LL SALT+HFFKR  EVLFVHKYSG M ++SAI++ LSY  S
Sbjct: 367 LVGLTSFFAFPHEDLRFGLLVSALTVHFFKRVVEVLFVHKYSGSMALESAIIVPLSYAFS 426

Query: 424 TALMIYTQHLSKSLPEPSIDLKNIGIALFLIGITGNMYHHYLLSQTRKKGETGYKIPKGG 483
           TA MIYTQ LS+ +PEP +DLK +G+ LF+IG  GN YHH+LL+  RKKG+  YKIPKGG
Sbjct: 427 TATMIYTQILSRDVPEPLLDLKYVGVGLFIIGEIGNFYHHWLLANLRKKGDKEYKIPKGG 486

Query: 484 LFSIVICPHYLCEITVYFGFALISQTLYSLCFAIATAIYLGGRSYATRKWYVSKF-DDFP 527
           LF +VICPHYL EI  Y G A ISQTLY+  F + T  YL GRSY TR+WY+SKF D FP
Sbjct: 487 LFDLVICPHYLFEILAYVGIASISQTLYAFSFTLGTIFYLTGRSYTTREWYLSKFGDKFP 546

BLAST of Lsi04G001740 vs. ExPASy TrEMBL
Match: A0A5B6WIM3 (3-oxo-5-alpha-steroid 4-dehydrogenase, C-terminal OS=Gossypium australe OX=47621 GN=EPI10_021869 PE=3 SV=1)

HSP 1 Score: 496.5 bits (1277), Expect = 1.4e-136
Identity = 264/525 (50.29%), Postives = 347/525 (66.10%), Query Frame = 0

Query: 6   EFVFPPPSSLFVNVMTVVSSAVVAVVGLSEARGKHMKYSKFWNTTNNAPNSSNLS----A 65
           + +FPP  +LF+   +V++   +A++G+ E RG ++KYSKF NT +++ +SS++S    +
Sbjct: 8   DILFPP--TLFLTGASVLTLLSLAILGVLEIRGINLKYSKFVNTASSSSSSSSISFKVPS 67

Query: 66  KLGMLLAYTPAFLVGIVSLWLFPNEDRRIFLLKSALILHFLKRNLEVVFLHKYSSKMVID 125
           ++GMLL YTPAFLVG+ S WL+P +D R   LKSA+ +H  KR  EV+F+HKYS KM +D
Sbjct: 68  RVGMLLLYTPAFLVGVASFWLYPADDSRFLFLKSAITIHSFKRLFEVIFIHKYSGKMSLD 127

Query: 126 TVITISFSYFSSTAIMIYTQHLSQGLPQPSIDLKNIGIALFSIGIIGNFYHHYLLSQARK 185
           T+I I  SYF  +  +IYTQ  +QGL +PSIDLK +G+ LF IGI GNFYHHYLLS+ R 
Sbjct: 128 TMIIILVSYFFLSLSLIYTQTFNQGLSEPSIDLKYLGLVLFLIGISGNFYHHYLLSKLRT 187

Query: 186 QGETSYKIPKGGLFSLVICPHYMFETIEYFGFALISQTIYSFFFAFATALYLSGRSYATR 245
           +G+  YKIPKGGLF LVICPHY+FE +E+ G +LISQT+YSF     +ALYL+ RSY TR
Sbjct: 188 KGDKEYKIPKGGLFELVICPHYLFEILEFLGISLISQTLYSFSVTLGSALYLTCRSYVTR 247

Query: 246 KWRNLLKKASPIQVRTAMNTLSAFLFPPPPSLFVNVMTVVSSAVVAVIGLSEARGTHLQY 305
           KW     +  P +V+     L  F F                                  
Sbjct: 248 KWYMSKFEDLPKEVK----ALVPFFF---------------------------------- 307

Query: 306 SKFWNTSNSATKSSNSEQLRISSRLGMFLLYTPAALAGAVSFWFFPNDDWRILLLKSALT 365
                   +A+ SS+S   ++ SR+GM L+YTPA L G  SFW FP+ D+R LLLKSA+T
Sbjct: 308 --------NASASSSSISFKVPSRVGMLLIYTPAFLVGVASFWLFPDGDFRFLLLKSAIT 367

Query: 366 LHFFKRDFEVLFVHKYSGKMVVDSAILISLSYFSSTALMIYTQHLSKSLPEPSIDLKNIG 425
           +HFFKR  EV+F+H YSG M +D+ I+I +SYF  +  +IYTQ  ++ L EPSIDLK +G
Sbjct: 368 IHFFKRILEVIFIHNYSGDMSLDTIIIILVSYFFVSLSLIYTQTFNQGLSEPSIDLKYLG 427

Query: 426 IALFLIGITGNMYHHYLLSQTRKKGETGYKIPKGGLFSIVICPHYLCEITVYFGFALISQ 485
           I LFLIGI GN YHH +LS+ R KG   YKIPKGGLF +VICPHYL EI  + G +LISQ
Sbjct: 428 IVLFLIGICGNFYHHCILSKLRTKGGKEYKIPKGGLFELVICPHYLFEILGFLGISLISQ 484

Query: 486 TLYSLCFAIATAIYLGGRSYATRKWYVSKFDDFPHHTKALIPFVF 527
           TLYS    +  A+YL  R Y TRKWY+SKF+DFP   KA+IP+VF
Sbjct: 488 TLYSFSTTLGIAVYLMCRGYVTRKWYMSKFEDFPKEVKAVIPYVF 484

BLAST of Lsi04G001740 vs. ExPASy TrEMBL
Match: A0A1S3BGE8 (3-oxo-5-alpha-steroid 4-dehydrogenase 1-like OS=Cucumis melo OX=3656 GN=LOC103489361 PE=3 SV=1)

HSP 1 Score: 477.2 bits (1227), Expect = 8.5e-131
Identity = 242/268 (90.30%), Postives = 248/268 (92.54%), Query Frame = 0

Query: 259 MNTLSAFLFPPPPSLFVNVMTVVSSAVVAVIGLSEARGTHLQYSKFWNTSNSATKSSNSE 318
           MN LS F FP PPSL VNVMTVVSSAVVAVIGLSEARGTHLQYSKFWNTSNSATKSSNSE
Sbjct: 1   MNALSVFFFPMPPSLLVNVMTVVSSAVVAVIGLSEARGTHLQYSKFWNTSNSATKSSNSE 60

Query: 319 QLRISSRLGMFLLYTPAALAGAVSFWFFPNDDWRILLLKSALTLHFFKRDFEVLFVHKYS 378
           QLRISSRLGM LLYTPAALAGA SFWFFPNDDWR+LLLKSALTLHFFKRDFEVLFVHKYS
Sbjct: 61  QLRISSRLGMLLLYTPAALAGAASFWFFPNDDWRVLLLKSALTLHFFKRDFEVLFVHKYS 120

Query: 379 GKMVVDSAILISLSYFSSTALMIYTQHLSKSLPEPSIDLKNIGIALFLIGITGNMYHHYL 438
           GKMV+DSA +ISLSYFSSTALMIY Q LSK+LPEP+IDLKNIGIALFLIGI GNMYHH L
Sbjct: 121 GKMVLDSASIISLSYFSSTALMIYAQQLSKALPEPTIDLKNIGIALFLIGIIGNMYHHIL 180

Query: 439 LSQTRKKGETGYKIPKGGLFSIVICPHYLCEITVYFGFALISQTLYSLCFAIATAIYLGG 498
           LSQTRKKGETGYKIPKGGLF IVICPHYL EITVYFGFALISQT YS  FAIATAIYLGG
Sbjct: 181 LSQTRKKGETGYKIPKGGLFGIVICPHYLFEITVYFGFALISQTFYSFFFAIATAIYLGG 240

Query: 499 RSYATRKWYVSKFDDFPHHTKALIPFVF 527
           RSYAT KWYVSKF+DFP H KALIPFVF
Sbjct: 241 RSYATWKWYVSKFEDFPQHIKALIPFVF 268

BLAST of Lsi04G001740 vs. ExPASy TrEMBL
Match: A0A166DSU9 (Uncharacterized protein OS=Daucus carota subsp. sativus OX=79200 GN=DCAR_006469 PE=3 SV=1)

HSP 1 Score: 474.6 bits (1220), Expect = 5.5e-130
Identity = 253/532 (47.56%), Postives = 331/532 (62.22%), Query Frame = 0

Query: 3   TLSEFVFPPPSSLFVNVMTVVSSAVVAVVGLSEARGKHMKYSKFWNTTN---NAPNSSNL 62
           TL  F++PPP+S F+N MTV++ A++   GL E +GK M+YSKF+NT+     A   + L
Sbjct: 5   TLLRFIYPPPASWFINAMTVITGAILVNGGLMEVKGKSMQYSKFYNTSKKNAEASKITEL 64

Query: 63  SAKLGMLLAYTPAFLVGIVSLWLFPNEDRRIFLLKSALILHFLKRNLEVVFLHKYSSKMV 122
           S + GML+ Y PAF+  ++ L    +E  R  L+ SAL +HF KR  EV F+H YS  M 
Sbjct: 65  SGRKGMLVVYAPAFVASVIFLAFMRDEGLRFTLVTSALTVHFFKRLFEVSFIHNYSGSMP 124

Query: 123 IDTVITISFSYFSSTAIMIYTQHLSQGLPQPSIDLKNIGIALFSIGIIGNFYHHYLLSQA 182
           ID VI  S SYF  T  + Y+QHL   LP+P IDLK  G A F +GI GNFYHHYLLS+ 
Sbjct: 125 IDIVINFSASYFLYTVTVTYSQHLVAELPEPVIDLKYAGTASFLVGITGNFYHHYLLSKL 184

Query: 183 RKQGETSYKIPKGGLFSLVICPHYMFETIEYFGFALISQTIYSFFFAFATALYLSGRSYA 242
           RK+GE  YKIP+G                                     A+ L GRSYA
Sbjct: 185 RKEGEKQYKIPQG-------------------------------------AVCLMGRSYA 244

Query: 243 TRKW-----RNLLKKASPIQVRTAMNTLSAFLFPPPPSLFVNVMTVVSSAVVAVIGLSEA 302
           TRKW      +  K    +     ++TL  F++PPP S F+  MTV++ A +   GL E 
Sbjct: 245 TRKWYLSKFEDFPKDVKALIPAMVLSTLLRFIYPPPASWFITAMTVINGASLVNAGLMEI 304

Query: 303 RGTHLQYSKFWNTSNSATKSSNSEQLRISSRLGMFLLYTPAALAGAVSFWFFPNDDWRIL 362
           +G  +QYSKF+NT+    ++S ++ + +S R GM + Y PA +A   S  F PN+  R  
Sbjct: 305 KGKSMQYSKFFNTNKKNVEASGAKIIELSGRNGMLVAYGPAFVASVTSLAFMPNEGLRFT 364

Query: 363 LLKSALTLHFFKRDFEVLFVHKYSGKMVVDSAILISLSYFSSTALMIYTQHLSKSLPEPS 422
           L+ SAL +HFFKR FEVLF+HKYSG M +DSAI+IS SYF S+   IY+QHL    PEP 
Sbjct: 365 LVSSALAVHFFKRLFEVLFIHKYSGSMPIDSAIVISTSYFLSSLCTIYSQHLCGQFPEPV 424

Query: 423 IDLKNIGIALFLIGITGNMYHHYLLSQTRKKGETGYKIPKGGLFSIVICPHYLCEITVYF 482
           +DLK  GI +FL+GI GN YHHYLLS+ RK+G+  YKIP+GGLF +VICPHYL EI  + 
Sbjct: 425 VDLKYAGITIFLVGIVGNFYHHYLLSKLRKEGDKEYKIPQGGLFDLVICPHYLFEILGFI 484

Query: 483 GFALISQTLYSLCFAIATAIYLGGRSYATRKWYVSKFDDFPHHTKALIPFVF 527
           G + +SQT+Y+L F   T  YL GRSYATR+WY+SKF+DFP   KALIP++F
Sbjct: 485 GISCMSQTVYALSFTAGTIGYLTGRSYATRRWYLSKFEDFPKDVKALIPYIF 499

BLAST of Lsi04G001740 vs. NCBI nr
Match: KAG7032032.1 (3-oxo-5-alpha-steroid 4-dehydrogenase 1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 708.4 bits (1827), Expect = 4.7e-200
Identity = 375/564 (66.49%), Postives = 428/564 (75.89%), Query Frame = 0

Query: 1   MDTLSEFVFPPPSSLFVNVMTVVSSAVVAVVGLSEARG-KHMKYSKFWNTTNNAPNSSN- 60
           MDT+S  +F PP SL VNVMTVVSS VVAV+GLSEARG  H++YSKFWN  N+A  SSN 
Sbjct: 4   MDTISALLFRPPPSLLVNVMTVVSSVVVAVIGLSEARGTAHLQYSKFWNANNSATKSSNP 63

Query: 61  ----LSAKLGMLLAYTPAFLVGIVSLWLFPNEDRRIFLLKSALILHFLKRNLEVVFLHKY 120
               +S++ GMLL Y PA L G VS W+FPN+D RI LLKSAL LHF+KRN EV+F+HKY
Sbjct: 64  EKFKISSRSGMLLLYAPAALAGAVSFWIFPNDDLRILLLKSALTLHFVKRNFEVLFIHKY 123

Query: 121 SSKMVIDTVITISFSYFSSTAIMIYTQHLSQGLPQPSIDLKNIGIALFSIGIIGNFYHHY 180
           S KM +D+ + IS SYFSSTA+MIY Q LS+ LP+PSIDLK IGI+LF IGIIGNFYHHY
Sbjct: 124 SGKMALDSALIISLSYFSSTALMIYAQELSRTLPEPSIDLKFIGISLFLIGIIGNFYHHY 183

Query: 181 LLSQARKQGETSYKIPKGGLFSLVICPHYMFETIEYFGFALISQTIYSFFFAFATALYLS 240
           +LSQ RK+GET YKIPKGGLF +VICPHY+FE   YFGFALISQTIYS  FA ATA+YL 
Sbjct: 184 ILSQTRKKGETGYKIPKGGLFCVVICPHYLFEITVYFGFALISQTIYSLCFAIATAMYLG 243

Query: 241 GRSYATRKW--------RNLLKKASP------------------------IQVRTAMNTL 300
           GRSYATRKW           +K   P                        I    AM  L
Sbjct: 244 GRSYATRKWYLSKFEDFPQHIKALVPFHHWFSARPEGVSKFYREFLAVFLIWFSAAMGIL 303

Query: 301 SAFLFPPPPSLFVNVMTVVSSAVVAVIGLSEARGTHLQYSKFWNTSNSATKSSNSEQLRI 360
           SAFLFPPP S FVNVMTVVSS  + + G+SE  G HLQYSKFWN +NS+ K+  SE+ ++
Sbjct: 304 SAFLFPPPASTFVNVMTVVSSTAMGMAGISEINGKHLQYSKFWN-ANSSPKAP-SEETKL 363

Query: 361 SSRLGMFLLYTPAALAGAVSFWFFPNDDWRILLLKSALTLHFFKRDFEVLFVHKYSGKMV 420
           SS+ GM L YTPA LAGA SFWFFP+D+ RIL+LK+ALTLHFFKR FEVLF+HKYS KMV
Sbjct: 364 SSKYGMLLAYTPAFLAGAASFWFFPSDNERILVLKAALTLHFFKRIFEVLFIHKYSSKMV 423

Query: 421 VDSAILISLSYFSSTALMIYTQHLSKSLPEPSIDLKNIGIALFLIGITGNMYHHYLLSQT 480
            D+AI IS SY  ST +MIYTQHLS+ LPEP IDLKNIGIALFLIGI GN YHH+LLS+T
Sbjct: 424 FDTAIKISFSYCLSTVIMIYTQHLSQGLPEPPIDLKNIGIALFLIGIIGNFYHHFLLSKT 483

Query: 481 RKKGETGYKIPKGGLFSIVICPHYLCEITVYFGFALISQTLYSLCFAIATAIYLGGRSYA 527
           RK GETGYKIPKGGLFS+VICPHYL EI  +FGFA ISQ LY LCF+  TAIYL GRSYA
Sbjct: 484 RKPGETGYKIPKGGLFSLVICPHYLFEIIEFFGFAFISQELYPLCFSTGTAIYLAGRSYA 543

BLAST of Lsi04G001740 vs. NCBI nr
Match: QCD94538.1 (enoyl reductase [Vigna unguiculata])

HSP 1 Score: 594.0 bits (1530), Expect = 1.3e-165
Identity = 296/526 (56.27%), Postives = 376/526 (71.48%), Query Frame = 0

Query: 1   MDTLSEFVFPPPSSLFVNVMTVVSSAVVAVVGLSEARGKHMKYSKFWNTTNNAPNSSNLS 60
           M T   F+F PP SL V+ ++VVS   +A  GLSE RGKH+ YSKFWN   ++     LS
Sbjct: 3   MSTFLSFIFAPPPSLVVSTLSVVSLVSLANAGLSEIRGKHLNYSKFWNANPSSEKQIKLS 62

Query: 61  AKLGMLLAYTPAFLVGIVSLWLFPNEDRRIFLLKSALILHFLKRNLEVVFLHKYSSKMVI 120
           +K GMLL YTPAFL  + S W+FP+E  R  LL+SA+ LHF KR+ EV+F+HKYS  M++
Sbjct: 63  SKTGMLLLYTPAFLAALASFWIFPHEGLRSTLLQSAVTLHFFKRDFEVLFIHKYSGGMLL 122

Query: 121 DTVITISFSYFSSTAIMIYTQHLSQGLPQPSIDLKNIGIALFSIGIIGNFYHHYLLSQAR 180
           D+ I I+ SYF S A M+Y QHL+QGLP+P I+L   GI +F +GIIGNFYHHYLLS  R
Sbjct: 123 DSAIPITLSYFLSAATMVYAQHLTQGLPEPPINLLYPGIVMFVVGIIGNFYHHYLLSNLR 182

Query: 181 KQGETSYKIPKGGLFSLVICPHYMFETIEYFGFALISQTIYSFFFAFATALYLSGRSYAT 240
            +GE  YKIPKGGLF +VICPHY+FE I+++G + ISQT+Y+F F   T  YL GRSY+T
Sbjct: 183 AKGEKEYKIPKGGLFGIVICPHYLFEIIQFYGISFISQTLYAFCFTLGTTFYLLGRSYST 242

Query: 241 RKWRNLLKKASPIQVRTAMNTLSAFLFPPPPSLFVNVMTVVSSAVVAVIGLSEARGTHLQ 300
           RKW    ++         M+ L +F+FPPPPS+ V+ +TV+S  ++    LSE +G HL 
Sbjct: 243 RKWSE--ERVELAYTAIVMSVLLSFMFPPPPSVVVSGLTVISFVILVSSFLSEIKGKHLN 302

Query: 301 YSKFWNTSNSATKSSNSEQLRISSRLGMFLLYTPAALAGAVSFWFFPNDDWRILLLKSAL 360
           YSKFWN + SA K     Q+++ S++GM LLYTPA LAG  SFW FP    R ++L+SA+
Sbjct: 303 YSKFWNANPSAQK-----QIKLPSKVGMLLLYTPAFLAGLASFWIFPPQSLRSIILQSAV 362

Query: 361 TLHFFKRDFEVLFVHKYSGKMVVDSAILISLSYFSSTALMIYTQHLSKSLPEPSIDLKNI 420
           T HFFKR FEVLF+HKYSG M ++SAI I+LSYF S A M Y+QHL+  LPEP ++L   
Sbjct: 363 TFHFFKRVFEVLFIHKYSGGMTLESAIPITLSYFLSAATMTYSQHLTNGLPEPPMNLLYP 422

Query: 421 GIALFLIGITGNMYHHYLLSQTRKKGETGYKIPKGGLFSIVICPHYLCEITVYFGFALIS 480
           G  +F++ I  N YHHYLLS  RK+GE  YKIPKGGLF +VICPHYL EI V++G   IS
Sbjct: 423 GTVIFVVAIICNFYHHYLLSNLRKEGEKEYKIPKGGLFGLVICPHYLFEIIVFYGIFFIS 482

Query: 481 QTLYSLCFAIATAIYLGGRSYATRKWYVSKFDDFPHHTKALIPFVF 527
           QTLY+  FAI T +YL GRSY+TRKWYVSKF+DFP + KA+IPFVF
Sbjct: 483 QTLYAFSFAIGTNLYLLGRSYSTRKWYVSKFEDFPQNVKAVIPFVF 521

BLAST of Lsi04G001740 vs. NCBI nr
Match: KAF9675046.1 (hypothetical protein SADUNF_Sadunf10G0190800 [Salix dunnii])

HSP 1 Score: 542.3 bits (1396), Expect = 4.5e-150
Identity = 285/527 (54.08%), Postives = 363/527 (68.88%), Query Frame = 0

Query: 4   LSEFVFPPPSSLFVNVMTVVSSAVVAVVGLSEARGKHMKYSKFWNTTNNAPNSSNL--SA 63
           L +  FPPP   F+  M+V+    +  VGLSE RGKHMKYSKF N     P    +  S+
Sbjct: 5   LLKLFFPPP--FFIKAMSVICVMSLGNVGLSEFRGKHMKYSKFLNVGEKKPIEKKILVSS 64

Query: 64  KLGMLLAYTPAFLVGIVSLWLFPNEDRRIFLLKSALILHFLKRNLEVVFLHKYSSKMVID 123
           + GML+AYTP+FLVG VS  +FPNED R  L+KS L  HF KR LEV+F+H+YS  M ++
Sbjct: 65  RTGMLIAYTPSFLVGFVSFGIFPNEDLRFLLVKSTLTFHFFKRILEVLFVHRYSGGMEVE 124

Query: 124 TVITISFSYFSSTAIMIYTQHLSQGLPQPSIDLKNIGIALFSIGIIGNFYHHYLLSQARK 183
           ++I I+ SYF+S+ ++IY QHL+QGLP+P+IDL   G+ LF IGI GN YHH LLS+ R 
Sbjct: 125 SLIPITLSYFTSSVLVIYAQHLAQGLPEPAIDLMYQGLILFLIGISGNLYHHCLLSKLRS 184

Query: 184 QGETSYKIPKGGLFSLVICPHYMFETIEYFGFALISQTIYSFFFAFATALYLSGRSYATR 243
             +T YK+PKGGLF LVICPHY+FE + + G +LI+QT+Y+F F   T LYL GRSY TR
Sbjct: 185 NKDTEYKVPKGGLFDLVICPHYLFEILGFLGISLIAQTLYAFSFTAGTTLYLMGRSYVTR 244

Query: 244 KWRNLLKKASPIQVRTAM--NTLSAFLFPPPPSLFVNVMTVVSSAVVAVIGLSEARGTHL 303
           +W     K  P  V+  +  + L   LF  P S  V  M+ V  A +  +GLSE RG H+
Sbjct: 245 RWYLSQFKDFPKDVKALIPCSMLLKLLF--PSSFLVKAMSAVPVATLGYVGLSEVRGKHM 304

Query: 304 QYSKFWNTSNSATKSSNSEQLRISSRLGMFLLYTPAALAGAVSFWFFPNDDWRILLLKSA 363
           QYSKF N      K+    ++++SSR GM + YTP  LAGA SF  FPNDD R  L+KS 
Sbjct: 305 QYSKFLNVGE---KNPIGNRIQVSSRNGMLIAYTPPFLAGAASFGIFPNDDIRFFLVKSI 364

Query: 364 LTLHFFKRDFEVLFVHKYSGKMVVDSAILISLSYFSSTALMIYTQHLSKSLPEPSIDLKN 423
           LT HFFKR  EVLFVH+YSG M V+S I I+LSYF+S+  +IY QHL++ LPEP+IDL  
Sbjct: 365 LTFHFFKRILEVLFVHRYSGGMEVESLIPITLSYFTSSVFVIYAQHLAQGLPEPAIDLMY 424

Query: 424 IGIALFLIGITGNMYHHYLLSQTRKKGETGYKIPKGGLFSIVICPHYLCEITVYFGFALI 483
            G+ LFLIGI+GN+YHH LLS+ R   +T YK+PKGGLF +VICPHYL EI    G +L 
Sbjct: 425 PGLMLFLIGISGNLYHHGLLSKLRSDKDTEYKVPKGGLFDLVICPHYLFEILGILGISLT 484

Query: 484 SQTLYSLCFAIATAIYLGGRSYATRKWYVSKFDDFPHHTKALIPFVF 527
           +QTLY+L F++ T +YL GRSY+TR+WY+S F DFP   KALIPFVF
Sbjct: 485 AQTLYALAFSVGTTLYLSGRSYSTRRWYLSHFKDFPKDVKALIPFVF 524

BLAST of Lsi04G001740 vs. NCBI nr
Match: KVI05075.1 (3-oxo-5-alpha-steroid 4-dehydrogenase, C-terminal [Cynara cardunculus var. scolymus])

HSP 1 Score: 527.7 bits (1358), Expect = 1.1e-145
Identity = 285/551 (51.72%), Postives = 359/551 (65.15%), Query Frame = 0

Query: 4   LSEFVFPPPSSLFVNVMTVVSSAVVAVVGLSEARGKHMKYSKFWNTTNNAPNSS-NLSAK 63
           L  F+FP PSS FV  M VV+ + +      E +GKH++YSKF N    A      LS++
Sbjct: 7   LLNFLFPSPSSWFVTAMLVVNLSALTDAAYMEIKGKHLRYSKFLNAGGVAKKQDMKLSSR 66

Query: 64  LGMLLAYTPAFLVGIVSLWLFPNEDRRIFLLKSALILHFLKRNLEVVFLHKYSSKMVIDT 123
            GMLL YTPAFLVG+ S ++ P +D R  LL SAL LHF KR LEV+FLHKYS  M +++
Sbjct: 67  NGMLLFYTPAFLVGVASFFVVPYQDLRFVLLASALTLHFFKRVLEVLFLHKYSGSMALES 126

Query: 124 VITISFSYFSSTAIMIYTQHLSQGLPQPSIDLKNIGIALFSIGIIGNFYHHYLLSQARKQ 183
            I I+ SY SSTA MIY Q LS+ +P+P++DLK +GI LF IG IGNFYHH LL+  R +
Sbjct: 127 PIIITMSYTSSTATMIYAQFLSRDVPEPTVDLKYVGIGLFFIGTIGNFYHHCLLANLRNK 186

Query: 184 GETSYKIPKGGLFSLVICPHYMFETIEYFGFALISQTIYSFFFAFATALYLSGRSYATRK 243
           G+  YKIP+GGLF LVICPHY+FE + Y G + ISQT Y+F F   T  YL GRSY TR 
Sbjct: 187 GDKEYKIPQGGLFGLVICPHYLFEILAYAGISSISQTPYAFAFTLGTIFYLIGRSYGTRG 246

Query: 244 W-----------------------RNLLKKASPIQVRTAMNTLSAF---LFPPPPSLFVN 303
           W                        NL +  + +++   M   S F   LFPPP SLFV 
Sbjct: 247 WYLSKFGDKFPKDVKALIPYCEFANNLRRTPNTLRLIIKMAIPSIFLNLLFPPPSSLFVT 306

Query: 304 VMTVVSSAVVAVIGLSEARGTHLQYSKFWNTSNSATKSSNSEQLRISSRLGMFLLYTPAA 363
            MTVV  + +   G  E +G H+QYSKF N      +    +++++SS+ GM L Y P+ 
Sbjct: 307 TMTVVGFSSLTNAGYKEIKGQHMQYSKFLNV--GVVEKEKEKEMKLSSKNGMLLFYAPSF 366

Query: 364 LAGAVSFWFFPNDDWRILLLKSALTLHFFKRDFEVLFVHKYSGKMVVDSAILISLSYFSS 423
           L G  SF+ FP++D R  LL SALT+HFFKR  EVLFVHKYSG M ++SAI++ LSY  S
Sbjct: 367 LVGLTSFFAFPHEDLRFGLLVSALTVHFFKRVVEVLFVHKYSGSMALESAIIVPLSYAFS 426

Query: 424 TALMIYTQHLSKSLPEPSIDLKNIGIALFLIGITGNMYHHYLLSQTRKKGETGYKIPKGG 483
           TA MIYTQ LS+ +PEP +DLK +G+ LF+IG  GN YHH+LL+  RKKG+  YKIPKGG
Sbjct: 427 TATMIYTQILSRDVPEPLLDLKYVGVGLFIIGEIGNFYHHWLLANLRKKGDKEYKIPKGG 486

Query: 484 LFSIVICPHYLCEITVYFGFALISQTLYSLCFAIATAIYLGGRSYATRKWYVSKF-DDFP 527
           LF +VICPHYL EI  Y G A ISQTLY+  F + T  YL GRSY TR+WY+SKF D FP
Sbjct: 487 LFDLVICPHYLFEILAYVGIASISQTLYAFSFTLGTIFYLTGRSYTTREWYLSKFGDKFP 546

BLAST of Lsi04G001740 vs. NCBI nr
Match: KAA3481510.1 (3-oxo-5-alpha-steroid 4-dehydrogenase, C-terminal [Gossypium australe])

HSP 1 Score: 496.5 bits (1277), Expect = 2.8e-136
Identity = 264/525 (50.29%), Postives = 347/525 (66.10%), Query Frame = 0

Query: 6   EFVFPPPSSLFVNVMTVVSSAVVAVVGLSEARGKHMKYSKFWNTTNNAPNSSNLS----A 65
           + +FPP  +LF+   +V++   +A++G+ E RG ++KYSKF NT +++ +SS++S    +
Sbjct: 8   DILFPP--TLFLTGASVLTLLSLAILGVLEIRGINLKYSKFVNTASSSSSSSSISFKVPS 67

Query: 66  KLGMLLAYTPAFLVGIVSLWLFPNEDRRIFLLKSALILHFLKRNLEVVFLHKYSSKMVID 125
           ++GMLL YTPAFLVG+ S WL+P +D R   LKSA+ +H  KR  EV+F+HKYS KM +D
Sbjct: 68  RVGMLLLYTPAFLVGVASFWLYPADDSRFLFLKSAITIHSFKRLFEVIFIHKYSGKMSLD 127

Query: 126 TVITISFSYFSSTAIMIYTQHLSQGLPQPSIDLKNIGIALFSIGIIGNFYHHYLLSQARK 185
           T+I I  SYF  +  +IYTQ  +QGL +PSIDLK +G+ LF IGI GNFYHHYLLS+ R 
Sbjct: 128 TMIIILVSYFFLSLSLIYTQTFNQGLSEPSIDLKYLGLVLFLIGISGNFYHHYLLSKLRT 187

Query: 186 QGETSYKIPKGGLFSLVICPHYMFETIEYFGFALISQTIYSFFFAFATALYLSGRSYATR 245
           +G+  YKIPKGGLF LVICPHY+FE +E+ G +LISQT+YSF     +ALYL+ RSY TR
Sbjct: 188 KGDKEYKIPKGGLFELVICPHYLFEILEFLGISLISQTLYSFSVTLGSALYLTCRSYVTR 247

Query: 246 KWRNLLKKASPIQVRTAMNTLSAFLFPPPPSLFVNVMTVVSSAVVAVIGLSEARGTHLQY 305
           KW     +  P +V+     L  F F                                  
Sbjct: 248 KWYMSKFEDLPKEVK----ALVPFFF---------------------------------- 307

Query: 306 SKFWNTSNSATKSSNSEQLRISSRLGMFLLYTPAALAGAVSFWFFPNDDWRILLLKSALT 365
                   +A+ SS+S   ++ SR+GM L+YTPA L G  SFW FP+ D+R LLLKSA+T
Sbjct: 308 --------NASASSSSISFKVPSRVGMLLIYTPAFLVGVASFWLFPDGDFRFLLLKSAIT 367

Query: 366 LHFFKRDFEVLFVHKYSGKMVVDSAILISLSYFSSTALMIYTQHLSKSLPEPSIDLKNIG 425
           +HFFKR  EV+F+H YSG M +D+ I+I +SYF  +  +IYTQ  ++ L EPSIDLK +G
Sbjct: 368 IHFFKRILEVIFIHNYSGDMSLDTIIIILVSYFFVSLSLIYTQTFNQGLSEPSIDLKYLG 427

Query: 426 IALFLIGITGNMYHHYLLSQTRKKGETGYKIPKGGLFSIVICPHYLCEITVYFGFALISQ 485
           I LFLIGI GN YHH +LS+ R KG   YKIPKGGLF +VICPHYL EI  + G +LISQ
Sbjct: 428 IVLFLIGICGNFYHHCILSKLRTKGGKEYKIPKGGLFELVICPHYLFEILGFLGISLISQ 484

Query: 486 TLYSLCFAIATAIYLGGRSYATRKWYVSKFDDFPHHTKALIPFVF 527
           TLYS    +  A+YL  R Y TRKWY+SKF+DFP   KA+IP+VF
Sbjct: 488 TLYSFSTTLGIAVYLMCRGYVTRKWYMSKFEDFPKEVKAVIPYVF 484

BLAST of Lsi04G001740 vs. TAIR 10
Match: AT5G16010.1 (3-oxo-5-alpha-steroid 4-dehydrogenase family protein )

HSP 1 Score: 323.6 bits (828), Expect = 3.0e-88
Identity = 166/269 (61.71%), Postives = 205/269 (76.21%), Query Frame = 0

Query: 259 MNTLSAFLFPPPPSLFVNVMTVVSSAVVAVIGLSEARGTHLQYSKFWNTSNSATKSSNSE 318
           M  +++F++PPPPS+ +N M+VV  A +A IG SE RG HL+YSKF   S+S+ +     
Sbjct: 1   MEMVTSFVYPPPPSILLNCMSVVGVAALANIGWSEIRGNHLKYSKF-GVSSSSPQPQKER 60

Query: 319 QLRISSRLGMFLLYTPAALAGAVSFWFFPNDDWRILLLKSALTLHFFKRDFEVLFVHKYS 378
              ISSR GM LLYTPA LA A SF+  P+DD R LLLKSAL LHFFKR FEVLF+HKYS
Sbjct: 61  FGSISSRNGMLLLYTPAFLAAASSFFVVPSDDLRFLLLKSALALHFFKRVFEVLFIHKYS 120

Query: 379 GKMVVDSAILISLSYFSSTALMIYTQHLSKSLPEPSIDLKNIGIALFLIGITGNMYHHYL 438
           G M +DSA+ IS SYFSSTALM+Y+Q+L+  L EPS D+K  G+ +F++GI GN+YHH L
Sbjct: 121 GGMAIDSALTISSSYFSSTALMLYSQNLTLGLTEPSFDMKLAGVVMFVVGIVGNLYHHVL 180

Query: 439 LSQTRKK-GETGYKIPKGGLFSIVICPHYLCEITVYFGFALISQTLYSLCFAIATAIYLG 498
           L++ RK+ G+  YKIPKGGLF I+ICPHYL EI V++ F LISQT+YS  FA+ T +YL 
Sbjct: 181 LAKLRKEDGKKEYKIPKGGLFDIIICPHYLFEILVFWSFFLISQTIYSFSFAMGTMLYLI 240

Query: 499 GRSYATRKWYVSKFDDFPHHTKALIPFVF 527
           GRSYATR WY+SKFDDFP H KALIPFVF
Sbjct: 241 GRSYATRTWYLSKFDDFPKHIKALIPFVF 268

BLAST of Lsi04G001740 vs. TAIR 10
Match: AT2G38050.1 (3-oxo-5-alpha-steroid 4-dehydrogenase family protein )

HSP 1 Score: 76.3 bits (186), Expect = 8.4e-14
Identity = 38/108 (35.19%), Postives = 66/108 (61.11%), Query Frame = 0

Query: 420 IGIALFLIGITGNMYHHYLLSQTRKKGETGYKIPKGGLFSIVICPHYLCEITVYFGFALI 479
           IG+ +F+ G+  N+     L + +K+   GY IP+GG F +V CP+Y  E   + G+A++
Sbjct: 155 IGMVVFITGMYINITSDRTLVRLKKENRGGYVIPRGGWFELVSCPNYFGEAIEWLGWAVM 214

Query: 480 SQTLYSLCFAIATAIYLGGRSYATRKWYVSKF-DDFPHHTKALIPFVF 527
           + +   + F + T   L  R+ A+ KWY++KF +++P   KA+IPFV+
Sbjct: 215 TWSWAGIGFFLYTCSNLFPRARASHKWYIAKFKEEYPKTRKAVIPFVY 262

BLAST of Lsi04G001740 vs. TAIR 10
Match: AT3G55360.1 (3-oxo-5-alpha-steroid 4-dehydrogenase family protein )

HSP 1 Score: 67.8 bits (164), Expect = 3.0e-11
Identity = 45/136 (33.09%), Postives = 67/136 (49.26%), Query Frame = 0

Query: 363 HFFKRDFEVLFVHKYSGKMVVDSAILISLSYFSSTALMIYTQHLSKSLPEPSIDLK-NIG 422
           H+FKR  E  FVH++S        +  + +Y+ S    I   +++  L  P  DL+  IG
Sbjct: 141 HYFKRILETFFVHRFSHATSPIGNVFRNCAYYWSFGAYI-AYYVNHPLYTPVSDLQMKIG 200

Query: 423 IALFLIGITGNMYHHYLLSQTR-KKGETGYKIPKGGLFSIVICPHYLCEITVYFGFALIS 482
               L+    N Y H LL   R   G  GY+IP+G LF+IV C +Y  EI  + GF + +
Sbjct: 201 FGFGLVCQVANFYCHILLKNLRDPSGAGGYQIPRGFLFNIVTCANYTTEIYQWLGFNIAT 260

Query: 483 QTLYSLCFAIATAIYL 497
           QT+    F    A+ +
Sbjct: 261 QTIAGYVFLAVAALIM 275

BLAST of Lsi04G001740 vs. TAIR 10
Match: AT1G72590.1 (3-oxo-5-alpha-steroid 4-dehydrogenase family protein )

HSP 1 Score: 58.5 bits (140), Expect = 1.8e-08
Identity = 39/117 (33.33%), Postives = 57/117 (48.72%), Query Frame = 0

Query: 417 LKNIGIALFLIGITGNMYHHYLLSQTRKKGETG--YKIPKGGLFSIVICPHYLCEITVYF 476
           L+ IG A+FL G       H +L   R+       Y IP G  F +V CPH+L EI +Y 
Sbjct: 207 LQWIGGAIFLWGWIHQRRCHAILGSLREYPSQAKEYIIPYGDWFEMVSCPHFLAEIVLYL 266

Query: 477 GFALISQ----TLYSLC-FAIATAIYLGGRSYATRKWYVSKFDDFPHHTKALIPFVF 527
           G  + S     +++ L  F  A   Y  G    T +WY+ KF+++P    A+ P V+
Sbjct: 267 GLLISSGGTDISIWLLFGFVAANLTYAAGE---THRWYLQKFENYPASRHAIFPHVY 320

BLAST of Lsi04G001740 vs. TAIR 10
Match: AT2G16530.1 (3-oxo-5-alpha-steroid 4-dehydrogenase family protein )

HSP 1 Score: 58.5 bits (140), Expect = 1.8e-08
Identity = 39/117 (33.33%), Postives = 58/117 (49.57%), Query Frame = 0

Query: 417 LKNIGIALFLIGITGNMYHHYLLSQTRKKGETG--YKIPKGGLFSIVICPHYLCEITVYF 476
           L+ IG A+FL G       H +L   R+       Y IP G  F +V  PH+L EI +Y 
Sbjct: 230 LQWIGGAIFLWGWIHQRRCHAILGSLRENPSQAKEYIIPYGDWFGMVSSPHFLAEIVLYA 289

Query: 477 GFALISQ----TLYSLC-FAIATAIYLGGRSYATRKWYVSKFDDFPHHTKALIPFVF 527
           G  + S     T++ L  F  A   Y  G    T +WY+ KF+++P +  A+ P+V+
Sbjct: 290 GLLIASGGTDITIWLLFGFVAANLTYAAGE---THRWYLRKFENYPANRHAIFPYVY 343

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q288911.9e-1842.063-oxo-5-alpha-steroid 4-dehydrogenase 1 OS=Macaca fascicularis OX=9541 GN=SRD5A1... [more]
Q288921.9e-1842.063-oxo-5-alpha-steroid 4-dehydrogenase 2 OS=Macaca fascicularis OX=9541 GN=SRD5A2... [more]
O187651.9e-1842.593-oxo-5-alpha-steroid 4-dehydrogenase 2 OS=Sus scrofa OX=9823 GN=SRD5A2 PE=2 SV=... [more]
P312134.2e-1840.743-oxo-5-alpha-steroid 4-dehydrogenase 2 OS=Homo sapiens OX=9606 GN=SRD5A2 PE=1 S... [more]
P184055.5e-1841.123-oxo-5-alpha-steroid 4-dehydrogenase 1 OS=Homo sapiens OX=9606 GN=SRD5A1 PE=1 S... [more]
Match NameE-valueIdentityDescription
A0A4D6M1Q76.3e-16656.27Enoyl reductase OS=Vigna unguiculata OX=3917 GN=DEO72_LG5g2622 PE=3 SV=1[more]
A0A103Y9N25.5e-14651.723-oxo-5-alpha-steroid 4-dehydrogenase, C-terminal OS=Cynara cardunculus var. sco... [more]
A0A5B6WIM31.4e-13650.293-oxo-5-alpha-steroid 4-dehydrogenase, C-terminal OS=Gossypium australe OX=47621... [more]
A0A1S3BGE88.5e-13190.303-oxo-5-alpha-steroid 4-dehydrogenase 1-like OS=Cucumis melo OX=3656 GN=LOC10348... [more]
A0A166DSU95.5e-13047.56Uncharacterized protein OS=Daucus carota subsp. sativus OX=79200 GN=DCAR_006469 ... [more]
Match NameE-valueIdentityDescription
KAG7032032.14.7e-20066.493-oxo-5-alpha-steroid 4-dehydrogenase 1 [Cucurbita argyrosperma subsp. argyrospe... [more]
QCD94538.11.3e-16556.27enoyl reductase [Vigna unguiculata][more]
KAF9675046.14.5e-15054.08hypothetical protein SADUNF_Sadunf10G0190800 [Salix dunnii][more]
KVI05075.11.1e-14551.723-oxo-5-alpha-steroid 4-dehydrogenase, C-terminal [Cynara cardunculus var. scoly... [more]
KAA3481510.12.8e-13650.293-oxo-5-alpha-steroid 4-dehydrogenase, C-terminal [Gossypium australe][more]
Match NameE-valueIdentityDescription
AT5G16010.13.0e-8861.713-oxo-5-alpha-steroid 4-dehydrogenase family protein [more]
AT2G38050.18.4e-1435.193-oxo-5-alpha-steroid 4-dehydrogenase family protein [more]
AT3G55360.13.0e-1133.093-oxo-5-alpha-steroid 4-dehydrogenase family protein [more]
AT1G72590.11.8e-0833.333-oxo-5-alpha-steroid 4-dehydrogenase family protein [more]
AT2G16530.11.8e-0833.333-oxo-5-alpha-steroid 4-dehydrogenase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR0011043-oxo-5-alpha-steroid 4-dehydrogenase, C-terminalPFAMPF02544Steroid_dhcoord: 417..526
e-value: 6.7E-29
score: 100.8
coord: 153..244
e-value: 3.3E-21
score: 75.8
IPR0011043-oxo-5-alpha-steroid 4-dehydrogenase, C-terminalPROSITEPS50244S5A_REDUCTASEcoord: 158..238
score: 26.275515
IPR0011043-oxo-5-alpha-steroid 4-dehydrogenase, C-terminalPROSITEPS50244S5A_REDUCTASEcoord: 421..501
score: 25.543774
NoneNo IPR availableGENE3D1.20.120.1630coord: 351..525
e-value: 2.7E-8
score: 35.8
NoneNo IPR availablePANTHERPTHR10556:SF353-OXO-5-ALPHA-STEROID 4-DEHYDROGENASE FAMILY PROTEINcoord: 7..243
coord: 264..526
IPR0393573-oxo-5-alpha-steroid 4-dehydrogenase/very-long-chain enoyl-CoA reductasePANTHERPTHR105563-OXO-5-ALPHA-STEROID 4-DEHYDROGENASEcoord: 7..243
coord: 264..526

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi04G001740.1Lsi04G001740.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006629 lipid metabolic process
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0016627 oxidoreductase activity, acting on the CH-CH group of donors