Lsi04G000230 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi04G000230
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	aspartyl protease AED3
Location	chr04: 335196 .. 339137 (-)
RNA-Seq Expression	Lsi04G000230
Synteny	Lsi04G000230

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TAATCATAATTTGACAAATAACAAAATGGTAAATTTACAAAGACTTGTGTAACTTAAGATAAGTCTTCTATAAATATCCCTTTCGAGTCTCCCCCACAAATCCAAATTACAAAACCCCAATTATCCCCTTCTTCACTTCCACACTCTGTCCTTTAATCCCAAAAATTGAAGCCATGAACACAAAATCCCCATTCCTCCTCTTTCTCCTCTTCCTCCTCCTCTCCGTTTCCGCCGAATCCCTCGCCGCTCATACCCCCAACTGCAACGCCGCCGCCGACCGGAGCTCCACCCTCCAAGTCTTCCACATTTTCAGCCCCTGCTCCCCCTTCCGCCCCTCCAAGCCGCTGTCATGGGCCGACAATGTGCTGCAAATGCAGGCCAAGGACCAAGCCCGGGTCCAGTTCCTGTCGTCCCTGGTGGCCCGGAGGTCCGTCGTCCCCATCGCCTCCGCCCGGCAGCTGATCCAGAGCCCCACCTTCGTGGTCCGGGCCAAGATCGGAACCCCCGCCCAAACCCTCCTCTTGGCCCTCGATACCAGCAACGACGCCGCTTGGATTCCTTGCTCCGGCTGCGTCGGTTGCCCTTCCGCCACCGTCTTCTCCTCCGATAAGTCCTCCTCCTTCCGCCCCCTCCCCTGCCAATCCCCTCAATGCAACCAGGTACCCCACCTCAGGCTCAATTCTATCTCTATTTCTATTTAATTCCCCCTTTTTTAATTTCTCACTTTTTTTTTTTTTTAAATTTTAATAATTTTCAACGGACAAAGGTGGGGTGAGAATTTAAGACATTTAAATATTTATTTTTGACATTGCATTTTTTCCCTAAAACCTTTTAAATCTTCTCGTTGAAGCTACATTTATAGTGTTTTTTTGTTTGTTTTTTTTTTTTTTTTTTTTAAAAAATCTTTAGAAGCAAAGATTTGAATGGTATTTGTTATGCTATTCAATACGCTTCTGAAGTTAAATATAAGGTTATAATTCGAATCTCTAATATTTTTGTCAACTGTAATTGTATAAGCCGGTTGAGTCATACTTAGATTGTCATTATTCAAAACTCCTTTATGCAACTATGTCCTTAACTATTTTTATTGAGTTGGAGGGTTTGAATCTCGCTTCCAATATTTATTGTACTAACAAAAAATTAACTAATACACATCCACATTGACCTTTTGAGTGTGTCATGGACTCATTGGTGTTTTGAAAACTTAGCTAAAAAATGTATACACTAAAATAGACCGGAAAAAGAAAAGGAAGAACAAGAAAGAACTTGAAAGTTCTAAGACCAAGGAGTATACAACTTTTTTTCTTTTTTTTTTTTTTTTTAAAAAAAAATAAAATAAAATATTTTTAACGATTTTGTAGCCGCCGTGATATCGAAGTGGTTTGTATTGGTGTAGAGGGCAGATATCCGTTGGAATATTCAATTATTATAAACATACAATAATTCACTTATATAACATTCCCCTTTTGGTTGCTAATCTGTGGGGTTCCCCTCTTTATGCTTTTCTTTCCATTTTTTAATATTCAAACCTACGCTTTTGGGGGAATTTCTTTTTTTACAAGTTTCGTCATTTAGTATAAACTTAAATTTTACATTATGCTTAATCAGTTCTTAAAATTTAAATTTAACTGTCTTATAAATTATTAGAATTTGTATTTTGAGGTCCATTTGAATTCACTTTTTAAGTGTTTAAATAAGTATTTATATGTGAAAAAAAAATTTATAAACACTTAAAAAGTCAATCTAAACGGACTCTCAAAATTTTTTATTTTATATAAAAATTCTCTAAAATAGTCTATTGGCCATAGATCATATCACTAATAGAATTTTATTCTATCACTGATAGGATTCTATTAACTATACAATTTATCTCCAATAAATTTTAGTATATTTACAATACTTTAAAAATATTATTATACATTTAATTATTAGTGCTAAAATTATAGTAACTATAATTTACAAAATTTAATAACAATTAAACTGTGTTTCTTTCTATCTAACAAACTAGGTTCATTTCTGTAAACTCCGATTGTTTTAGCGCGTAGACCAACTTAAAATTCAGAAAGATTTATTCTTTATAATTACATTATAACCATAACCAATCGAGATTTGCATTGGGACTTTGGATGCTTTTCTAGAATCCAACCCATGTTCCCACGTGCCGAACAGTCATTTCCTTCGAGGAGACACGTGTGATCAAAACCCCTGATTTAATTTCCGTACGACTTAAAAAGAAAAAAGTCCTTAATTTTAAATTTTTGGGTGTCTCTAGAATCTATGATTGTCAGTGAGTCACCGACGGGGGCAGCGTTGAGTCACGGGCCTAAAGAAAAAACTTTTCCCAAAAAAATCCTTGTCGTATTGGAGGTATCACGTTAACAGCACGTGACCCTTTTTATTTCTCAGGTACCGAACCCGAGTTGCACCGGCAGCGCGTGCGGTTTCAACCTGACGTACGGCAGCTCCACGGTGGCGGCGGATCTGGTTCAAGACAACATAACTCTGGCCACAGACTCAGTCCCGGCGTACACATTTGGTTGCATCACGAAGGCGACGGGTAGTTCAGTACCGCCGCAGGGGCTATTGGGCTTGGGCCGCGGCCCGTTATCGCTTTTGGGGCAGAGCCAGAGCTTGTACCAATCCACATTCTCGTACTGTCTTCCGAGCTTCAAATCGGTTAACTTTTCTGGGTCGTTGCGTCTTGGGCCTGTGGCCCAGCCCATTAGGATTAAGTACACGCCGTTGCTCCGGAACCCGCGGCGGTCGTCGTTGTATTATGTGAATTTGATTGCCATTAGAGTCGGCCGGAGAATCGTCGATATTCCTCCCTCTGCTCTCGCTTTTAACTCCGTCACCGGCGCCGGCACTATCATCGATTCCGGTATCGTTTTCTTCTCACTCTGTTCATTACGAATTTACGATTTGGCCTACAACTCCGCACCTTTTATGTCTATTTTGGTCTTTTGACTTTTGAATTACTTTTCATTTGCTCTCTAGAGACTAAACTAAGGACCATAGGGATATTAAATGTTTGTTTTCTCATGAAAAAGAAAAAAAAGAAAAGTAAAAATTTGGATTGATGGTTGTTGTTCAGGGACAACGTTCACAAGGCTGGTGGCACCGGCGTACACGGCGGTGAGGGACGAATTCCGACGAAGAGTGGGGAGAAACATAACAGTGTCATCCCTGGGGGGGTTTGACACCTGCTACACAGTCCCCATCATCTCTCCGACGATAACCTTGATGTTCGCGGGAATGAACGTCACTCTTCCGCAGGACAACTTCCTCATCCACAGCACCGCGGGATCCACCACCTGCCTGGCCATGGCCGCCGCCCCGGATAACGTGAACTCCGTACTCAATGTGATTGCCAGTATGCAGCAGCAAAACCACCGCATTCTCTTCGACATTCCCAATTCCCGCCTCGGCGTTGCTCGTGAACCCTGCTCTTCTTAGTTAACACACTCGTTAGAGGTTGCTGTTGTCGTTCGTCTCAGATTCTGCTGTTGGTTTCTAGAATAAGAAATGTGTGGGTGTAAACTAGTAATTTTGAATTGTTTTTTCTTTCTTTCTTTCTTTCTTCTTCTTTTTTATTTTAAAGTACAATGAATGAAGTAAAGAGAGGGAAATACATGCCAATTACAAGTGAGTTATGGTCATTTTGGCATTTAAAATGGATATTGAAAGAGTGAGATTTTAGAGAGAAAAAAAATGTATTTTGTATGGTTGGTGTTTTGTAGTAGTGTCATTTGGGATTTGGAAGGTTTTAGCCATATTATTGTCTCAAAAAGCAAAGCTTTTCTTTTTCTTTTTCTTTTTCTTTTTATTTTTTTACTACAATTCTCCTGTGTTGTGTAAGTTTACTACTATTAAGTATTAACAGAGTAGCTATATGACAGACAGAGTGATTAACTTTTATCATTGAAATCTTTTTGGAAAAAA

mRNA sequence

TAATCATAATTTGACAAATAACAAAATGGTAAATTTACAAAGACTTGTGTAACTTAAGATAAGTCTTCTATAAATATCCCTTTCGAGTCTCCCCCACAAATCCAAATTACAAAACCCCAATTATCCCCTTCTTCACTTCCACACTCTGTCCTTTAATCCCAAAAATTGAAGCCATGAACACAAAATCCCCATTCCTCCTCTTTCTCCTCTTCCTCCTCCTCTCCGTTTCCGCCGAATCCCTCGCCGCTCATACCCCCAACTGCAACGCCGCCGCCGACCGGAGCTCCACCCTCCAAGTCTTCCACATTTTCAGCCCCTGCTCCCCCTTCCGCCCCTCCAAGCCGCTGTCATGGGCCGACAATGTGCTGCAAATGCAGGCCAAGGACCAAGCCCGGGTCCAGTTCCTGTCGTCCCTGGTGGCCCGGAGGTCCGTCGTCCCCATCGCCTCCGCCCGGCAGCTGATCCAGAGCCCCACCTTCGTGGTCCGGGCCAAGATCGGAACCCCCGCCCAAACCCTCCTCTTGGCCCTCGATACCAGCAACGACGCCGCTTGGATTCCTTGCTCCGGCTGCGTCGGTTGCCCTTCCGCCACCGTCTTCTCCTCCGATAAGTCCTCCTCCTTCCGCCCCCTCCCCTGCCAATCCCCTCAATGCAACCAGGTACCGAACCCGAGTTGCACCGGCAGCGCGTGCGGTTTCAACCTGACGTACGGCAGCTCCACGGTGGCGGCGGATCTGGTTCAAGACAACATAACTCTGGCCACAGACTCAGTCCCGGCGTACACATTTGGTTGCATCACGAAGGCGACGGGTAGTTCAGTACCGCCGCAGGGGCTATTGGGCTTGGGCCGCGGCCCGTTATCGCTTTTGGGGCAGAGCCAGAGCTTGTACCAATCCACATTCTCGTACTGTCTTCCGAGCTTCAAATCGGTTAACTTTTCTGGGTCGTTGCGTCTTGGGCCTGTGGCCCAGCCCATTAGGATTAAGTACACGCCGTTGCTCCGGAACCCGCGGCGGTCGTCGTTGTATTATGTGAATTTGATTGCCATTAGAGTCGGCCGGAGAATCGTCGATATTCCTCCCTCTGCTCTCGCTTTTAACTCCGTCACCGGCGCCGGCACTATCATCGATTCCGGGACAACGTTCACAAGGCTGGTGGCACCGGCGTACACGGCGGTGAGGGACGAATTCCGACGAAGAGTGGGGAGAAACATAACAGTGTCATCCCTGGGGGGGTTTGACACCTGCTACACAGTCCCCATCATCTCTCCGACGATAACCTTGATGTTCGCGGGAATGAACGTCACTCTTCCGCAGGACAACTTCCTCATCCACAGCACCGCGGGATCCACCACCTGCCTGGCCATGGCCGCCGCCCCGGATAACGTGAACTCCGTACTCAATGTGATTGCCAGTATGCAGCAGCAAAACCACCGCATTCTCTTCGACATTCCCAATTCCCGCCTCGGCGTTGCTCGTGAACCCTGCTCTTCTTAGTTAACACACTCGTTAGAGGTTGCTGTTGTCGTTCGTCTCAGATTCTGCTGTTGGTTTCTAGAATAAGAAATGTGTGGGTGTAAACTAGTAATTTTGAATTGTTTTTTCTTTCTTTCTTTCTTTCTTCTTCTTTTTTATTTTAAAGTACAATGAATGAAGTAAAGAGAGGGAAATACATGCCAATTACAAGTGAGTTATGGTCATTTTGGCATTTAAAATGGATATTGAAAGAGTGAGATTTTAGAGAGAAAAAAAATGTATTTTGTATGGTTGGTGTTTTGTAGTAGTGTCATTTGGGATTTGGAAGGTTTTAGCCATATTATTGTCTCAAAAAGCAAAGCTTTTCTTTTTCTTTTTCTTTTTCTTTTTATTTTTTTACTACAATTCTCCTGTGTTGTGTAAGTTTACTACTATTAAGTATTAACAGAGTAGCTATATGACAGACAGAGTGATTAACTTTTATCATTGAAATCTTTTTGGAAAAAA

Coding sequence (CDS)

ATGAACACAAAATCCCCATTCCTCCTCTTTCTCCTCTTCCTCCTCCTCTCCGTTTCCGCCGAATCCCTCGCCGCTCATACCCCCAACTGCAACGCCGCCGCCGACCGGAGCTCCACCCTCCAAGTCTTCCACATTTTCAGCCCCTGCTCCCCCTTCCGCCCCTCCAAGCCGCTGTCATGGGCCGACAATGTGCTGCAAATGCAGGCCAAGGACCAAGCCCGGGTCCAGTTCCTGTCGTCCCTGGTGGCCCGGAGGTCCGTCGTCCCCATCGCCTCCGCCCGGCAGCTGATCCAGAGCCCCACCTTCGTGGTCCGGGCCAAGATCGGAACCCCCGCCCAAACCCTCCTCTTGGCCCTCGATACCAGCAACGACGCCGCTTGGATTCCTTGCTCCGGCTGCGTCGGTTGCCCTTCCGCCACCGTCTTCTCCTCCGATAAGTCCTCCTCCTTCCGCCCCCTCCCCTGCCAATCCCCTCAATGCAACCAGGTACCGAACCCGAGTTGCACCGGCAGCGCGTGCGGTTTCAACCTGACGTACGGCAGCTCCACGGTGGCGGCGGATCTGGTTCAAGACAACATAACTCTGGCCACAGACTCAGTCCCGGCGTACACATTTGGTTGCATCACGAAGGCGACGGGTAGTTCAGTACCGCCGCAGGGGCTATTGGGCTTGGGCCGCGGCCCGTTATCGCTTTTGGGGCAGAGCCAGAGCTTGTACCAATCCACATTCTCGTACTGTCTTCCGAGCTTCAAATCGGTTAACTTTTCTGGGTCGTTGCGTCTTGGGCCTGTGGCCCAGCCCATTAGGATTAAGTACACGCCGTTGCTCCGGAACCCGCGGCGGTCGTCGTTGTATTATGTGAATTTGATTGCCATTAGAGTCGGCCGGAGAATCGTCGATATTCCTCCCTCTGCTCTCGCTTTTAACTCCGTCACCGGCGCCGGCACTATCATCGATTCCGGGACAACGTTCACAAGGCTGGTGGCACCGGCGTACACGGCGGTGAGGGACGAATTCCGACGAAGAGTGGGGAGAAACATAACAGTGTCATCCCTGGGGGGGTTTGACACCTGCTACACAGTCCCCATCATCTCTCCGACGATAACCTTGATGTTCGCGGGAATGAACGTCACTCTTCCGCAGGACAACTTCCTCATCCACAGCACCGCGGGATCCACCACCTGCCTGGCCATGGCCGCCGCCCCGGATAACGTGAACTCCGTACTCAATGTGATTGCCAGTATGCAGCAGCAAAACCACCGCATTCTCTTCGACATTCCCAATTCCCGCCTCGGCGTTGCTCGTGAACCCTGCTCTTCTTAG

Protein sequence

MNTKSPFLLFLLFLLLSVSAESLAAHTPNCNAAADRSSTLQVFHIFSPCSPFRPSKPLSWADNVLQMQAKDQARVQFLSSLVARRSVVPIASARQLIQSPTFVVRAKIGTPAQTLLLALDTSNDAAWIPCSGCVGCPSATVFSSDKSSSFRPLPCQSPQCNQVPNPSCTGSACGFNLTYGSSTVAADLVQDNITLATDSVPAYTFGCITKATGSSVPPQGLLGLGRGPLSLLGQSQSLYQSTFSYCLPSFKSVNFSGSLRLGPVAQPIRIKYTPLLRNPRRSSLYYVNLIAIRVGRRIVDIPPSALAFNSVTGAGTIIDSGTTFTRLVAPAYTAVRDEFRRRVGRNITVSSLGGFDTCYTVPIISPTITLMFAGMNVTLPQDNFLIHSTAGSTTCLAMAAAPDNVNSVLNVIASMQQQNHRILFDIPNSRLGVAREPCSS

Homology

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BLAST of Lsi04G000230 vs. ExPASy Swiss-Prot
Match: O04496 (Aspartyl protease AED3 OS=Arabidopsis thaliana OX=3702 GN=AED3 PE=1 SV=1)

HSP 1 Score: 409.1 bits (1050), Expect = 6.5e-113
Identity = 228/449 (50.78%), Postives = 299/449 (66.59%), Query Frame = 0


Query: 5   SPFLLFLLFLLLSVSAESLAAHTPNC-NAAADRSSTLQVFHIFSPCSPFRPSK-PLSWAD 64
           S  L F  FL L +      A    C  AA D S  L +  I + CSPF P+    S  D
Sbjct: 3   SSSLHFFFFLTLLLPFTFTTATRDTCATAAPDGSDDLSIIPINAKCSPFAPTHVSASVID 62

Query: 65  NVLQMQAKDQARVQFLSSLVA---RRSVVPIASARQLIQSPTFVVRAKIGTPAQTLLLAL 124
            VL M + D  R+ +LSSLVA   + + VP+AS  QL     +VVRAK+GTP Q + + L
Sbjct: 63  TVLHMASSDSHRLTYLSSLVAGKPKPTSVPVASGNQL-HIGNYVVRAKLGTPPQLMFMVL 122

Query: 125 DTSNDAAWIPCSGCVGCPSA-TVFSSDKSSSFRPLPCQSPQCNQV-----PNPSCTGSAC 184
           DTSNDA W+PCSGC GC +A T F+++ SS++  + C + QC Q      P+ S   S C
Sbjct: 123 DTSNDAVWLPCSGCSGCSNASTSFNTNSSSTYSTVSCSTAQCTQARGLTCPSSSPQPSVC 182

Query: 185 GFNLTY-GSSTVAADLVQDNITLATDSVPAYTFGCITKATGSSVPPQGLLGLGRGPLSLL 244
            FN +Y G S+ +A LVQD +TLA D +P ++FGCI  A+G+S+PPQGL+GLGRGP+SL+
Sbjct: 183 SFNQSYGGDSSFSASLVQDTLTLAPDVIPNFSFGCINSASGNSLPPQGLMGLGRGPMSLV 242

Query: 245 GQSQSLYQSTFSYCLPSFKSVNFSGSLRLGPVAQPIRIKYTPLLRNPRRSSLYYVNLIAI 304
            Q+ SLY   FSYCLPSF+S  FSGSL+LG + QP  I+YTPLLRNPRR SLYYVNL  +
Sbjct: 243 SQTTSLYSGVFSYCLPSFRSFYFSGSLKLGLLGQPKSIRYTPLLRNPRRPSLYYVNLTGV 302

Query: 305 RVGRRIVDIPPSALAFNSVTGAGTIIDSGTTFTRLVAPAYTAVRDEFRRRVGRNITVSSL 364
            VG   V + P  L F++ +GAGTIIDSGT  TR   P Y A+RDEFR++V  + + S+L
Sbjct: 303 SVGSVQVPVDPVYLTFDANSGAGTIIDSGTVITRFAQPVYEAIRDEFRKQVNVS-SFSTL 362

Query: 365 GGFDTCYTV--PIISPTITLMFAGMNVTLPQDNFLIHSTAGSTTCLAMAAAPDNVNSVLN 424
           G FDTC++     ++P ITL    +++ LP +N LIHS+AG+ TCL+MA    N N+VLN
Sbjct: 363 GAFDTCFSADNENVAPKITLHMTSLDLKLPMENTLIHSSAGTLTCLSMAGIRQNANAVLN 422

Query: 425 VIASMQQQNHRILFDIPNSRLGVAREPCS 440
           VIA++QQQN RILFD+PNSR+G+A EPC+
Sbjct: 423 VIANLQQQNLRILFDVPNSRIGIAPEPCN 449

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BLAST of Lsi04G000230 vs. ExPASy Swiss-Prot
Match: Q6F4N5 (Aspartyl protease 25 OS=Oryza sativa subsp. japonica OX=39947 GN=AP25 PE=2 SV=1)

HSP 1 Score: 355.1 bits (910), Expect = 1.1e-96
Identity = 206/453 (45.47%), Postives = 279/453 (61.59%), Query Frame = 0


Query: 11  LLFLLLSVSAESLAAHTPNCNAAADRSSTLQVFHIFSPCSPFRPSKPLSWADNVLQMQAK 70
           LL LLL+ +  + AA              L V+H   P SP     PL   ++++ +   
Sbjct: 9   LLLLLLAATVAAAAAE-------------LSVYHNVHPSSP----SPL---ESIIALARD 68

Query: 71  DQARVQFLSSLVARRSV--VPIASARQLIQSPTFVVRAKIGTPAQTLLLALDTSNDAAWI 130
           D AR+ FLSS  A   V   P+AS +     P++VVRA +G+P+Q LLLALDTS DA W 
Sbjct: 69  DDARLLFLSSKAATAGVSSAPVASGQ---APPSYVVRAGLGSPSQQLLLALDTSADATWA 128

Query: 131 PCSGCVGCPSATVFSSDKSSSFRPLPCQSPQC-----NQVPNPSCTGSA---------CG 190
            CS C  CPS+++F+   SSS+  LPC S  C        P P   G A         C 
Sbjct: 129 HCSPCGTCPSSSLFAPANSSSYASLPCSSSWCPLFQGQACPAPQGGGDAAPPPATLPTCA 188

Query: 191 FNLTYGSSTVAADLVQDNITLATDSVPAYTFGCITKATG--SSVPPQGLLGLGRGPLSLL 250
           F+  +  ++  A L  D + L  D++P YTFGC++  TG  +++P QGLLGLGRGP++LL
Sbjct: 189 FSKPFADASFQAALASDTLRLGKDAIPNYTFGCVSSVTGPTTNMPRQGLLGLGRGPMALL 248

Query: 251 GQSQSLYQSTFSYCLPSFKSVNFSGSLRLGP-VAQPIRIKYTPLLRNPRRSSLYYVNLIA 310
            Q+ SLY   FSYCLPS++S  FSGSLRLG    QP  ++YTP+LRNP RSSLYYVN+  
Sbjct: 249 SQAGSLYNGVFSYCLPSYRSYYFSGSLRLGAGGGQPRSVRYTPMLRNPHRSSLYYVNVTG 308

Query: 311 IRVGRRIVDIPPSALAFNSVTGAGTIIDSGTTFTRLVAPAYTAVRDEFRRRVGRNITVSS 370
           + VG   V +P  + AF++ TGAGT++DSGT  TR  AP Y A+R+EFRR+V      +S
Sbjct: 309 LSVGHAWVKVPAGSFAFDAATGAGTVVDSGTVITRWTAPVYAALREEFRRQVAAPSGYTS 368

Query: 371 LGGFDTCYTVPII----SPTITL-MFAGMNVTLPQDNFLIHSTAGSTTCLAMAAAPDNVN 430
           LG FDTC+    +    +P +T+ M  G+++ LP +N LIHS+A    CLAMA AP NVN
Sbjct: 369 LGAFDTCFNTDEVAAGGAPAVTVHMDGGVDLALPMENTLIHSSATPLACLAMAEAPQNVN 428

Query: 431 SVLNVIASMQQQNHRILFDIPNSRLGVAREPCS 440
           SV+NVIA++QQQN R++FD+ NSR+G A+E C+
Sbjct: 429 SVVNVIANLQQQNIRVVFDVANSRVGFAKESCN 438

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BLAST of Lsi04G000230 vs. ExPASy Swiss-Prot
Match: Q9LNJ3 (Aspartyl protease family protein 2 OS=Arabidopsis thaliana OX=3702 GN=APF2 PE=2 SV=1)

HSP 1 Score: 192.2 bits (487), Expect = 1.2e-47
Identity = 117/351 (33.33%), Postives = 176/351 (50.14%), Query Frame = 0


Query: 99  SPTFVVRAKIGTPAQTLLLALDTSNDAAWIPCSGCVGCPSAT--VFSSDKSSSFRPLPCQ 158
           S  +  R  +GTPA+ + + LDT +D  W+ C+ C  C S +  +F   KS ++  +PC 
Sbjct: 139 SGEYFTRLGVGTPARYVYMVLDTGSDIVWLQCAPCRRCYSQSDPIFDPRKSKTYATIPCS 198

Query: 159 SPQCNQVPNPSCT--GSACGFNLTYG-SSTVAADLVQDNITLATDSVPAYTFGCITKATG 218
           SP C ++ +  C      C + ++YG  S    D   + +T   + V     GC     G
Sbjct: 199 SPHCRRLDSAGCNTRRKTCLYQVSYGDGSFTVGDFSTETLTFRRNRVKGVALGCGHDNEG 258

Query: 219 SSVPPQGLLGLGRGPLSLLGQSQSLYQSTFSYCLPSFKSVNFSGSLRLGPVAQPIRIKYT 278
             V   GLLGLG+G LS  GQ+   +   FSYCL    + +   S+  G  A     ++T
Sbjct: 259 LFVGAAGLLGLGKGKLSFPGQTGHRFNQKFSYCLVDRSASSKPSSVVFGNAAVSRIARFT 318

Query: 279 PLLRNPRRSSLYYVNLIAIRV-GRRIVDIPPSALAFNSVTGAGTIIDSGTTFTRLVAPAY 338
           PLL NP+  + YYV L+ I V G R+  +  S    + +   G IIDSGT+ TRL+ PAY
Sbjct: 319 PLLSNPKLDTFYYVGLLGISVGGTRVPGVTASLFKLDQIGNGGVIIDSGTSVTRLIRPAY 378

Query: 339 TAVRDEFRRRVGRNITVSSLGGFDTCYTVPIIS----PTITLMFAGMNVTLPQDNFLIHS 398
            A+RD FR              FDTC+ +  ++    PT+ L F G +V+LP  N+LI  
Sbjct: 379 IAMRDAFRVGAKTLKRAPDFSLFDTCFDLSNMNEVKVPTVVLHFRGADVSLPATNYLIPV 438

Query: 399 TAGSTTCLAMAAAPDNVNSVLNVIASMQQQNHRILFDIPNSRLGVAREPCS 440
                 C A A         L++I ++QQQ  R+++D+ +SR+G A   C+
Sbjct: 439 DTNGKFCFAFAGTMGG----LSIIGNIQQQGFRVVYDLASSRVGFAPGGCA 485

[Close]

BLAST of Lsi04G000230 vs. ExPASy Swiss-Prot
Match: Q9LHE3 (Protein ASPARTIC PROTEASE IN GUARD CELL 2 OS=Arabidopsis thaliana OX=3702 GN=ASPG2 PE=2 SV=1)

HSP 1 Score: 176.4 bits (446), Expect = 7.1e-43
Identity = 112/348 (32.18%), Postives = 164/348 (47.13%), Query Frame = 0


Query: 99  SPTFVVRAKIGTPAQTLLLALDTSNDAAWIPCSGCVGC--PSATVFSSDKSSSFRPLPCQ 158
           S  + VR  +G+P +   + +D+ +D  W+ C  C  C   S  VF   KS S+  + C 
Sbjct: 128 SGEYFVRIGVGSPPRDQYMVIDSGSDMVWVQCQPCKLCYKQSDPVFDPAKSGSYTGVSCG 187

Query: 159 SPQCNQVPNPSCTGSACGFNLTYG-SSTVAADLVQDNITLATDSVPAYTFGCITKATGSS 218
           S  C+++ N  C    C + + YG  S     L  + +T A   V     GC  +  G  
Sbjct: 188 SSVCDRIENSGCHSGGCRYEVMYGDGSYTKGTLALETLTFAKTVVRNVAMGCGHRNRGMF 247

Query: 219 VPPQGLLGLGRGPLSLLGQSQSLYQSTFSYCLPSFKSVNFSGSLRLGPVAQPIRIKYTPL 278
           +   GLLG+G G +S +GQ        F YCL S +  + +GSL  G  A P+   + PL
Sbjct: 248 IGAAGLLGIGGGSMSFVGQLSGQTGGAFGYCLVS-RGTDSTGSLVFGREALPVGASWVPL 307

Query: 279 LRNPRRSSLYYVNLIAIRVGRRIVDIPPSALAFNSVTGAGTIIDSGTTFTRLVAPAYTAV 338
           +RNPR  S YYV L  + VG   + +P            G ++D+GT  TRL   AY A 
Sbjct: 308 VRNPRAPSFYYVGLKGLGVGGVRIPLPDGVFDLTETGDGGVVMDTGTAVTRLPTAAYVAF 367

Query: 339 RDEFRRRVGRNITVSSLGGFDTCYT----VPIISPTITLMFA-GMNVTLPQDNFLIHSTA 398
           RD F+ +       S +  FDTCY     V +  PT++  F  G  +TLP  NFL+    
Sbjct: 368 RDGFKSQTANLPRASGVSIFDTCYDLSGFVSVRVPTVSFYFTEGPVLTLPARNFLMPVDD 427

Query: 399 GSTTCLAMAAAPDNVNSVLNVIASMQQQNHRILFDIPNSRLGVAREPC 439
             T C A AA+P      L++I ++QQ+  ++ FD  N  +G     C
Sbjct: 428 SGTYCFAFAASPTG----LSIIGNIQQEGIQVSFDGANGFVGFGPNVC 470

[Close]

BLAST of Lsi04G000230 vs. ExPASy Swiss-Prot
Match: Q9LEW3 (Aspartyl protease AED1 OS=Arabidopsis thaliana OX=3702 GN=AED1 PE=2 SV=1)

HSP 1 Score: 174.9 bits (442), Expect = 2.1e-42
Identity = 137/443 (30.93%), Postives = 209/443 (47.18%), Query Frame = 0


Query: 17  SVSAESLAAHTPNC---NAAADRSSTLQVFHIFSPCSPFRPSKPLSWADNVLQMQAKDQA 76
           ++   SL   + +C   + A++  S+L+V H+   CS       +   D +++   +DQA
Sbjct: 39  TIQVSSLFPSSSSCVPSSKASNTKSSLRVVHMHGACSHLSSDARVD-HDEIIR---RDQA 98

Query: 77  RVQFLSSLVARRSVVPIASARQ---------LIQSPTFVVRAKIGTPAQTLLLALDTSND 136
           RV+ + S +++ S   ++ A+           + S  ++V   IGTP   L L  DT +D
Sbjct: 99  RVESIYSKLSKNSANEVSEAKSTELPAKSGITLGSGNYIVTIGIGTPKHDLSLVFDTGSD 158

Query: 137 AAWIPCSGCVG-CPS--ATVFSSDKSSSFRPLPCQSPQCNQVPNPSCTGSACGFNLTYG- 196
             W  C  C+G C S     F+   SS+++ + C SP C      SC+ S C +++ YG 
Sbjct: 159 LTWTQCEPCLGSCYSQKEPKFNPSSSSTYQNVSCSSPMCEDA--ESCSASNCVYSIVYGD 218

Query: 197 SSTVAADLVQDNITLA-TDSVPAYTFGCITKATGSSVPPQGLLGLGRGPLSLLGQSQSLY 256
            S     L ++  TL  +D +    FGC     G      GLLGLG G LSL  Q+ + Y
Sbjct: 219 KSFTQGFLAKEKFTLTNSDVLEDVYFGCGENNQGLFDGVAGLLGLGPGKLSLPAQTTTTY 278

Query: 257 QSTFSYCLPSFKSVNFSGSLRLGPVAQPIRIKYTPLLRNPRRSSLYYVNLIAIRVGRRIV 316
            + FSYCLPSF S N +G L  G       +K+TP+   P   + Y +++I I VG + +
Sbjct: 279 NNIFSYCLPSFTS-NSTGHLTFGSAGISESVKFTPISSFPSAFN-YGIDIIGISVGDKEL 338

Query: 317 DIPPSALAFNSVTGAGTIIDSGTTFTRLVAPAYTAVRDEFRRRVGRNITVSSLGGFDTCY 376
            I P     NS +  G IIDSGT FTRL    Y  +R  F+ ++    + S  G FDTCY
Sbjct: 339 AITP-----NSFSTEGAIIDSGTVFTRLPTKVYAELRSVFKEKMSSYKSTSGYGLFDTCY 398

Query: 377 TV----PIISPTITLMFAGMNVTLPQDNFLIHSTAGSTTCLAMAAAPDNVNSVLNVIASM 436
                  +  PTI   FAG  V     + +      S  CLA A   D    +  +  ++
Sbjct: 399 DFTGLDTVTYPTIAFSFAGSTVVELDGSGISLPIKISQVCLAFAGNDD----LPAIFGNV 458

Query: 437 QQQNHRILFDIPNSRLGVAREPC 439
           QQ    +++D+   R+G A   C
Sbjct: 459 QQTTLDVVYDVAGGRVGFAPNGC 464

[Close]

BLAST of Lsi04G000230 vs. ExPASy TrEMBL
Match: A0A5A7SU62 (Aspartyl protease AED3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold475G003150 PE=4 SV=1)

HSP 1 Score: 803.1 bits (2073), Expect = 5.7e-229
Identity = 412/442 (93.21%), Postives = 422/442 (95.48%), Query Frame = 0


Query: 2   NTKSPF--LLFLLFLLLSVSAESLAAHTP-NCNAAADRSSTLQVFHIFSPCSPFRPSKPL 61
           NTKSPF  L  LL LLL  S  +  +H P NCN A DRSSTL+VFHIFSPCSPFRPSKPL
Sbjct: 3   NTKSPFLPLPLLLLLLLLFSISTAKSHIPLNCNPADDRSSTLKVFHIFSPCSPFRPSKPL 62

Query: 62  SWADNVLQMQAKDQARVQFLSSLVARRSVVPIASARQLIQSPTFVVRAKIGTPAQTLLLA 121
           SWADNVLQMQAKDQAR+QFLSSLVARRSVVPIASARQLIQSPTFVVRAKIGTPAQTLLLA
Sbjct: 63  SWADNVLQMQAKDQARLQFLSSLVARRSVVPIASARQLIQSPTFVVRAKIGTPAQTLLLA 122

Query: 122 LDTSNDAAWIPCSGCVGCPSATVFSSDKSSSFRPLPCQSPQCNQVPNPSCTGSACGFNLT 181
           LDTSNDAAWIPCSGCVGCPS TVFSSDKSSSFRPLPCQSPQCNQVPNPSC+G+ACGFNLT
Sbjct: 123 LDTSNDAAWIPCSGCVGCPSTTVFSSDKSSSFRPLPCQSPQCNQVPNPSCSGTACGFNLT 182

Query: 182 YGSSTVAADLVQDNITLATDSVPAYTFGCITKATGSSVPPQGLLGLGRGPLSLLGQSQSL 241
           YGSSTVAADLVQDN+TLATDSVPAYTFGCI KATGSSVPPQGLLGLGRGPLSLLGQSQSL
Sbjct: 183 YGSSTVAADLVQDNVTLATDSVPAYTFGCIRKATGSSVPPQGLLGLGRGPLSLLGQSQSL 242

Query: 242 YQSTFSYCLPSFKSVNFSGSLRLGPVAQPIRIKYTPLLRNPRRSSLYYVNLIAIRVGRRI 301
           Y+STFSYCLPSFKSVNFSGSLRLGPVAQPIRIKYTPLLRNPRRSSLYYVNLIAIRVGRRI
Sbjct: 243 YRSTFSYCLPSFKSVNFSGSLRLGPVAQPIRIKYTPLLRNPRRSSLYYVNLIAIRVGRRI 302

Query: 302 VDIPPSALAFNSVTGAGTIIDSGTTFTRLVAPAYTAVRDEFRRRVGRNITVSSLGGFDTC 361
           VDIPPSALAFNS TGAGTIIDSGTTFTRLVAPAYTAVRDEFRRRVGRN+TVSSLGGFDTC
Sbjct: 303 VDIPPSALAFNSATGAGTIIDSGTTFTRLVAPAYTAVRDEFRRRVGRNVTVSSLGGFDTC 362

Query: 362 YTVPIISPTITLMFAGMNVTLPQDNFLIHSTAGSTTCLAMAAAPDNVNSVLNVIASMQQQ 421
           YTVPIISPTIT MFAGMNVTLP DNFLIHSTAGSTTCLAMAAAPDNVNSVLNVIASMQQQ
Sbjct: 363 YTVPIISPTITFMFAGMNVTLPPDNFLIHSTAGSTTCLAMAAAPDNVNSVLNVIASMQQQ 422

Query: 422 NHRILFDIPNSRLGVAREPCSS 441
           NHRILFDIPNSR+GVAREPCSS
Sbjct: 423 NHRILFDIPNSRVGVAREPCSS 444

[Close]

BLAST of Lsi04G000230 vs. ExPASy TrEMBL
Match: A0A1S3BGB2 (aspartyl protease AED3 OS=Cucumis melo OX=3656 GN=LOC103489519 PE=4 SV=1)

HSP 1 Score: 803.1 bits (2073), Expect = 5.7e-229
Identity = 412/442 (93.21%), Postives = 422/442 (95.48%), Query Frame = 0


Query: 2   NTKSPF--LLFLLFLLLSVSAESLAAHTP-NCNAAADRSSTLQVFHIFSPCSPFRPSKPL 61
           NTKSPF  L  LL LLL  S  +  +H P NCN A DRSSTL+VFHIFSPCSPFRPSKPL
Sbjct: 3   NTKSPFLPLPLLLLLLLLFSISTAKSHIPLNCNPADDRSSTLKVFHIFSPCSPFRPSKPL 62

Query: 62  SWADNVLQMQAKDQARVQFLSSLVARRSVVPIASARQLIQSPTFVVRAKIGTPAQTLLLA 121
           SWADNVLQMQAKDQAR+QFLSSLVARRSVVPIASARQLIQSPTFVVRAKIGTPAQTLLLA
Sbjct: 63  SWADNVLQMQAKDQARLQFLSSLVARRSVVPIASARQLIQSPTFVVRAKIGTPAQTLLLA 122

Query: 122 LDTSNDAAWIPCSGCVGCPSATVFSSDKSSSFRPLPCQSPQCNQVPNPSCTGSACGFNLT 181
           LDTSNDAAWIPCSGCVGCPS TVFSSDKSSSFRPLPCQSPQCNQVPNPSC+G+ACGFNLT
Sbjct: 123 LDTSNDAAWIPCSGCVGCPSTTVFSSDKSSSFRPLPCQSPQCNQVPNPSCSGTACGFNLT 182

Query: 182 YGSSTVAADLVQDNITLATDSVPAYTFGCITKATGSSVPPQGLLGLGRGPLSLLGQSQSL 241
           YGSSTVAADLVQDN+TLATDSVPAYTFGCI KATGSSVPPQGLLGLGRGPLSLLGQSQSL
Sbjct: 183 YGSSTVAADLVQDNVTLATDSVPAYTFGCIRKATGSSVPPQGLLGLGRGPLSLLGQSQSL 242

Query: 242 YQSTFSYCLPSFKSVNFSGSLRLGPVAQPIRIKYTPLLRNPRRSSLYYVNLIAIRVGRRI 301
           Y+STFSYCLPSFKSVNFSGSLRLGPVAQPIRIKYTPLLRNPRRSSLYYVNLIAIRVGRRI
Sbjct: 243 YRSTFSYCLPSFKSVNFSGSLRLGPVAQPIRIKYTPLLRNPRRSSLYYVNLIAIRVGRRI 302

Query: 302 VDIPPSALAFNSVTGAGTIIDSGTTFTRLVAPAYTAVRDEFRRRVGRNITVSSLGGFDTC 361
           VDIPPSALAFNS TGAGTIIDSGTTFTRLVAPAYTAVRDEFRRRVGRN+TVSSLGGFDTC
Sbjct: 303 VDIPPSALAFNSATGAGTIIDSGTTFTRLVAPAYTAVRDEFRRRVGRNVTVSSLGGFDTC 362

Query: 362 YTVPIISPTITLMFAGMNVTLPQDNFLIHSTAGSTTCLAMAAAPDNVNSVLNVIASMQQQ 421
           YTVPIISPTIT MFAGMNVTLP DNFLIHSTAGSTTCLAMAAAPDNVNSVLNVIASMQQQ
Sbjct: 363 YTVPIISPTITFMFAGMNVTLPPDNFLIHSTAGSTTCLAMAAAPDNVNSVLNVIASMQQQ 422

Query: 422 NHRILFDIPNSRLGVAREPCSS 441
           NHRILFDIPNSR+GVAREPCSS
Sbjct: 423 NHRILFDIPNSRVGVAREPCSS 444

[Close]

BLAST of Lsi04G000230 vs. ExPASy TrEMBL
Match: A0A0A0KTR5 (Aspartic proteinase nepenthesin-1 OS=Cucumis sativus OX=3659 GN=Csa_5G623870 PE=4 SV=1)

HSP 1 Score: 801.2 bits (2068), Expect = 2.2e-228
Identity = 409/440 (92.95%), Postives = 421/440 (95.68%), Query Frame = 0


Query: 2   NTKSPFLLFLLFLLLSVSAESLAAHTP-NCNAAADRSSTLQVFHIFSPCSPFRPSKPLSW 61
           NTKSPFLL LL L L  S  +  +H P NCN AADRSSTLQVFHIFSPCSPFRPSKPLSW
Sbjct: 3   NTKSPFLLLLLLLHL-FSISTAKSHIPSNCNPAADRSSTLQVFHIFSPCSPFRPSKPLSW 62

Query: 62  ADNVLQMQAKDQARVQFLSSLVARRSVVPIASARQLIQSPTFVVRAKIGTPAQTLLLALD 121
           ADNVLQMQAKDQAR+QFLSSLVARRS VPIASARQLIQSPTFVVRAKIGTPAQTLLLALD
Sbjct: 63  ADNVLQMQAKDQARLQFLSSLVARRSFVPIASARQLIQSPTFVVRAKIGTPAQTLLLALD 122

Query: 122 TSNDAAWIPCSGCVGCPSATVFSSDKSSSFRPLPCQSPQCNQVPNPSCTGSACGFNLTYG 181
           TSNDAAWIPCSGC+GCPS TVFSSDKSSSFRPLPCQSPQCNQVPNPSC+GSACGFNLTYG
Sbjct: 123 TSNDAAWIPCSGCIGCPSTTVFSSDKSSSFRPLPCQSPQCNQVPNPSCSGSACGFNLTYG 182

Query: 182 SSTVAADLVQDNITLATDSVPAYTFGCITKATGSSVPPQGLLGLGRGPLSLLGQSQSLYQ 241
           SSTVAADLVQDN+TLATDSVP+YTFGCI KATGSSVPPQGLLGLGRGPLSLLGQSQSLYQ
Sbjct: 183 SSTVAADLVQDNLTLATDSVPSYTFGCIRKATGSSVPPQGLLGLGRGPLSLLGQSQSLYQ 242

Query: 242 STFSYCLPSFKSVNFSGSLRLGPVAQPIRIKYTPLLRNPRRSSLYYVNLIAIRVGRRIVD 301
           STFSYCLPSFKSVNFSGSLRLGPVAQPIRIKYTPLLRNPRRSSLYYVNLI+IRVGR+IVD
Sbjct: 243 STFSYCLPSFKSVNFSGSLRLGPVAQPIRIKYTPLLRNPRRSSLYYVNLISIRVGRKIVD 302

Query: 302 IPPSALAFNSVTGAGTIIDSGTTFTRLVAPAYTAVRDEFRRRVGRNITVSSLGGFDTCYT 361
           IPPSALAFNS TGAGT+IDSGTTFTRLVAPAYTAVRDEFRRRVGRN+TVSSLGGFDTCYT
Sbjct: 303 IPPSALAFNSATGAGTVIDSGTTFTRLVAPAYTAVRDEFRRRVGRNVTVSSLGGFDTCYT 362

Query: 362 VPIISPTITLMFAGMNVTLPQDNFLIHSTAGSTTCLAMAAAPDNVNSVLNVIASMQQQNH 421
           VPIISPTIT MFAGMNVTLP DNFLIHSTAGSTTCLAMAAAPDNVNSVLNVIASMQQQNH
Sbjct: 363 VPIISPTITFMFAGMNVTLPPDNFLIHSTAGSTTCLAMAAAPDNVNSVLNVIASMQQQNH 422

Query: 422 RILFDIPNSRLGVAREPCSS 441
           RILFDIPNSR+GVARE CSS
Sbjct: 423 RILFDIPNSRVGVARESCSS 441

[Close]

BLAST of Lsi04G000230 vs. ExPASy TrEMBL
Match: A0A6J1GYP2 (aspartyl protease AED3 OS=Cucurbita moschata OX=3662 GN=LOC111458686 PE=4 SV=1)

HSP 1 Score: 793.5 bits (2048), Expect = 4.5e-226
Identity = 405/439 (92.26%), Postives = 420/439 (95.67%), Query Frame = 0


Query: 1   MNTKSPFLLFLLFLLLSVSAESLAAHTPNCNAAADRSSTLQVFHIFSPCSPFRPSKPLSW 60
           M TKSPFLL LL LLLS+SAES   H PNCNAAADRSSTLQVFHIFSPCSPFRPSKPLSW
Sbjct: 1   MKTKSPFLLSLL-LLLSISAESHHHHHPNCNAAADRSSTLQVFHIFSPCSPFRPSKPLSW 60

Query: 61  ADNVLQMQAKDQARVQFLSSLVARRSVVPIASARQLIQSPTFVVRAKIGTPAQTLLLALD 120
           ADNVLQMQAKDQAR+QFLSSLVAR+SV PIASARQLIQSPTFVVRAKIGTPAQTLL+ALD
Sbjct: 61  ADNVLQMQAKDQARLQFLSSLVARKSVAPIASARQLIQSPTFVVRAKIGTPAQTLLMALD 120

Query: 121 TSNDAAWIPCSGCVGCPSATVFSSDKSSSFRPLPCQSPQCNQVPNPSCTGSACGFNLTYG 180
           TSNDAAWIPCSGCVGCPS TVFSSDKSSSFRPLPC+SPQCNQVPNPSC+ SACGFNLTYG
Sbjct: 121 TSNDAAWIPCSGCVGCPSTTVFSSDKSSSFRPLPCRSPQCNQVPNPSCSSSACGFNLTYG 180

Query: 181 SSTVAADLVQDNITLATDSVPAYTFGCITKATGSSVPPQGLLGLGRGPLSLLGQSQSLYQ 240
           SSTVAADLVQDNITLA DSVPAYTFGCITKATGSSVPPQGLLGLGRGPLSLLGQSQSLY+
Sbjct: 181 SSTVAADLVQDNITLANDSVPAYTFGCITKATGSSVPPQGLLGLGRGPLSLLGQSQSLYR 240

Query: 241 STFSYCLPSFKSVNFSGSLRLGPVAQPIRIKYTPLLRNPRRSSLYYVNLIAIRVGRRIVD 300
           STFSYCLPSFKS  FSGSLRLGPVAQP RIKYTPLLRNPRRSSLYYVNLIAIRVGRRIVD
Sbjct: 241 STFSYCLPSFKSTGFSGSLRLGPVAQPKRIKYTPLLRNPRRSSLYYVNLIAIRVGRRIVD 300

Query: 301 IPPSALAFNSVTGAGTIIDSGTTFTRLVAPAYTAVRDEFRRRVGRNITVSSLGGFDTCYT 360
           IPPSALAFNS TGAGTIIDSGTTFTRLVAPAYTAVR+EFRRR+G N T+SSLGGFDTCYT
Sbjct: 301 IPPSALAFNSATGAGTIIDSGTTFTRLVAPAYTAVRNEFRRRIG-NATISSLGGFDTCYT 360

Query: 361 VPIISPTITLMFAGMNVTLPQDNFLIHSTAGSTTCLAMAAAPDNVNSVLNVIASMQQQNH 420
           VPIISPTIT MFAGMNVTLP DNFL+HS+AG+TTCLAMAAAPDNVNSVLNVIASMQQQNH
Sbjct: 361 VPIISPTITFMFAGMNVTLPPDNFLLHSSAGTTTCLAMAAAPDNVNSVLNVIASMQQQNH 420

Query: 421 RILFDIPNSRLGVAREPCS 440
           RILFD+PNSR+GVAREPCS
Sbjct: 421 RILFDVPNSRIGVAREPCS 437

[Close]

BLAST of Lsi04G000230 vs. ExPASy TrEMBL
Match: A0A6J1JIM3 (aspartyl protease AED3-like OS=Cucurbita maxima OX=3661 GN=LOC111486144 PE=4 SV=1)

HSP 1 Score: 792.3 bits (2045), Expect = 1.0e-225
Identity = 404/440 (91.82%), Postives = 421/440 (95.68%), Query Frame = 0


Query: 1   MNTKSPFLLFLLFLLLSVSAESL-AAHTPNCNAAADRSSTLQVFHIFSPCSPFRPSKPLS 60
           M TKSPFLL LL LLLS+SAESL   H PNCNAAADRSSTLQVFHIFSPCSPFRPSKPLS
Sbjct: 1   MKTKSPFLLSLLLLLLSISAESLHHHHHPNCNAAADRSSTLQVFHIFSPCSPFRPSKPLS 60

Query: 61  WADNVLQMQAKDQARVQFLSSLVARRSVVPIASARQLIQSPTFVVRAKIGTPAQTLLLAL 120
           WADNVLQMQAKDQAR+QFLSSLVA +SV PIASARQLIQSPTFVVRAKIGTPAQTLL+AL
Sbjct: 61  WADNVLQMQAKDQARLQFLSSLVACKSVAPIASARQLIQSPTFVVRAKIGTPAQTLLMAL 120

Query: 121 DTSNDAAWIPCSGCVGCPSATVFSSDKSSSFRPLPCQSPQCNQVPNPSCTGSACGFNLTY 180
           DTSNDAAWIPCSGCVGCPS TVFSSDKS+SFRPLPC+SPQCNQVPNPSC+ SACGFNLTY
Sbjct: 121 DTSNDAAWIPCSGCVGCPSTTVFSSDKSTSFRPLPCRSPQCNQVPNPSCSSSACGFNLTY 180

Query: 181 GSSTVAADLVQDNITLATDSVPAYTFGCITKATGSSVPPQGLLGLGRGPLSLLGQSQSLY 240
           GSSTVAADLVQDNITLA DSVPAYTFGCITKATGSSVPPQGLLGLGRGPLSLLGQSQSLY
Sbjct: 181 GSSTVAADLVQDNITLANDSVPAYTFGCITKATGSSVPPQGLLGLGRGPLSLLGQSQSLY 240

Query: 241 QSTFSYCLPSFKSVNFSGSLRLGPVAQPIRIKYTPLLRNPRRSSLYYVNLIAIRVGRRIV 300
           +STFSYCLPSFKS +FSGSLRLGPVAQP RIKYTPLLRNPRRSSLYYVNLIAIRVGRRIV
Sbjct: 241 RSTFSYCLPSFKSTSFSGSLRLGPVAQPKRIKYTPLLRNPRRSSLYYVNLIAIRVGRRIV 300

Query: 301 DIPPSALAFNSVTGAGTIIDSGTTFTRLVAPAYTAVRDEFRRRVGRNITVSSLGGFDTCY 360
           DIPPSALAFNS TGAGTIIDSGTTFTRLVAPAYTAVR+EFRRR+G N T+SSLGGFDTCY
Sbjct: 301 DIPPSALAFNSATGAGTIIDSGTTFTRLVAPAYTAVRNEFRRRIG-NATISSLGGFDTCY 360

Query: 361 TVPIISPTITLMFAGMNVTLPQDNFLIHSTAGSTTCLAMAAAPDNVNSVLNVIASMQQQN 420
           TVPIISPTIT MFAGMNVTLP DNFL+HS+AG+TTCLAMAAAPDNVNSVLNVIASMQQQN
Sbjct: 361 TVPIISPTITFMFAGMNVTLPPDNFLLHSSAGTTTCLAMAAAPDNVNSVLNVIASMQQQN 420

Query: 421 HRILFDIPNSRLGVAREPCS 440
           HRILFD+PNSR+GVAREPCS
Sbjct: 421 HRILFDVPNSRMGVAREPCS 439

[Close]

BLAST of Lsi04G000230 vs. NCBI nr
Match: XP_038892592.1 (aspartyl protease AED3 [Benincasa hispida])

HSP 1 Score: 818.1 bits (2112), Expect = 3.5e-233
Identity = 421/441 (95.46%), Postives = 427/441 (96.83%), Query Frame = 0


Query: 1   MNTKSPFLL-FLLFLLLSVSAESLAAHTPNCNAAADRSSTLQVFHIFSPCSPFRPSKPLS 60
           MNTKSPFLL  LLFLLLS+SAESL  HTPNCN +ADR+STLQVFHIFSPCSPFRPSKPLS
Sbjct: 36  MNTKSPFLLSLLLFLLLSISAESLHRHTPNCNPSADRTSTLQVFHIFSPCSPFRPSKPLS 95

Query: 61  WADNVLQMQAKDQARVQFLSSLVARRSVVPIASARQLIQSPTFVVRAKIGTPAQTLLLAL 120
             DNVLQ+QAKDQAR+ FLSSLVARRSVVPIASARQLIQSPTFVVRAKIGTPAQTLLLAL
Sbjct: 96  SPDNVLQLQAKDQARLHFLSSLVARRSVVPIASARQLIQSPTFVVRAKIGTPAQTLLLAL 155

Query: 121 DTSNDAAWIPCSGCVGCPSATVFSSDKSSSFRPLPCQSPQCNQVPNPSCTGSACGFNLTY 180
           DTSNDAAWIPCSGCVGCPSATVFSSDKSSSFRPLPCQS QCNQVPNPSC+GSACGFNLTY
Sbjct: 156 DTSNDAAWIPCSGCVGCPSATVFSSDKSSSFRPLPCQSSQCNQVPNPSCSGSACGFNLTY 215

Query: 181 GSSTVAADLVQDNITLATDSVPAYTFGCITKATGSSVPPQGLLGLGRGPLSLLGQSQSLY 240
           GSSTVAADLVQDNITLATDSVP YTFGCITKATGSSVPPQGLLGLGRGPLSLLGQSQSLY
Sbjct: 216 GSSTVAADLVQDNITLATDSVPGYTFGCITKATGSSVPPQGLLGLGRGPLSLLGQSQSLY 275

Query: 241 QSTFSYCLPSFKSVNFSGSLRLGPVAQPIRIKYTPLLRNPRRSSLYYVNLIAIRVGRRIV 300
           QSTFSYCLPSFKSVNFSGSLRLGPVAQPIRIKYTPLLRNPRRSSLYYVNLIAIRVGRRIV
Sbjct: 276 QSTFSYCLPSFKSVNFSGSLRLGPVAQPIRIKYTPLLRNPRRSSLYYVNLIAIRVGRRIV 335

Query: 301 DIPPSALAFNSVTGAGTIIDSGTTFTRLVAPAYTAVRDEFRRRVGRNITVSSLGGFDTCY 360
           DIPPSALAFNSVTGAGTIIDSGTTFTRLVAPAYTAVRDEFRRRVGRNITVSSLGGFDTCY
Sbjct: 336 DIPPSALAFNSVTGAGTIIDSGTTFTRLVAPAYTAVRDEFRRRVGRNITVSSLGGFDTCY 395

Query: 361 TVPIISPTITLMFAGMNVTLPQDNFLIHSTAGSTTCLAMAAAPDNVNSVLNVIASMQQQN 420
           TVPIISPTIT MFAGMNVTLP DNFLIHSTAGSTTCLAMAAAPDNVNS LNVIASMQQQN
Sbjct: 396 TVPIISPTITFMFAGMNVTLPPDNFLIHSTAGSTTCLAMAAAPDNVNSALNVIASMQQQN 455

Query: 421 HRILFDIPNSRLGVAREPCSS 441
           HRILFDIPNSRLGVARE CSS
Sbjct: 456 HRILFDIPNSRLGVARERCSS 476

[Close]

BLAST of Lsi04G000230 vs. NCBI nr
Match: XP_008446966.1 (PREDICTED: aspartyl protease AED3 [Cucumis melo] >KAA0034764.1 aspartyl protease AED3 [Cucumis melo var. makuwa] >TYK09318.1 aspartyl protease AED3 [Cucumis melo var. makuwa])

HSP 1 Score: 803.1 bits (2073), Expect = 1.2e-228
Identity = 412/442 (93.21%), Postives = 422/442 (95.48%), Query Frame = 0


Query: 2   NTKSPF--LLFLLFLLLSVSAESLAAHTP-NCNAAADRSSTLQVFHIFSPCSPFRPSKPL 61
           NTKSPF  L  LL LLL  S  +  +H P NCN A DRSSTL+VFHIFSPCSPFRPSKPL
Sbjct: 3   NTKSPFLPLPLLLLLLLLFSISTAKSHIPLNCNPADDRSSTLKVFHIFSPCSPFRPSKPL 62

Query: 62  SWADNVLQMQAKDQARVQFLSSLVARRSVVPIASARQLIQSPTFVVRAKIGTPAQTLLLA 121
           SWADNVLQMQAKDQAR+QFLSSLVARRSVVPIASARQLIQSPTFVVRAKIGTPAQTLLLA
Sbjct: 63  SWADNVLQMQAKDQARLQFLSSLVARRSVVPIASARQLIQSPTFVVRAKIGTPAQTLLLA 122

Query: 122 LDTSNDAAWIPCSGCVGCPSATVFSSDKSSSFRPLPCQSPQCNQVPNPSCTGSACGFNLT 181
           LDTSNDAAWIPCSGCVGCPS TVFSSDKSSSFRPLPCQSPQCNQVPNPSC+G+ACGFNLT
Sbjct: 123 LDTSNDAAWIPCSGCVGCPSTTVFSSDKSSSFRPLPCQSPQCNQVPNPSCSGTACGFNLT 182

Query: 182 YGSSTVAADLVQDNITLATDSVPAYTFGCITKATGSSVPPQGLLGLGRGPLSLLGQSQSL 241
           YGSSTVAADLVQDN+TLATDSVPAYTFGCI KATGSSVPPQGLLGLGRGPLSLLGQSQSL
Sbjct: 183 YGSSTVAADLVQDNVTLATDSVPAYTFGCIRKATGSSVPPQGLLGLGRGPLSLLGQSQSL 242

Query: 242 YQSTFSYCLPSFKSVNFSGSLRLGPVAQPIRIKYTPLLRNPRRSSLYYVNLIAIRVGRRI 301
           Y+STFSYCLPSFKSVNFSGSLRLGPVAQPIRIKYTPLLRNPRRSSLYYVNLIAIRVGRRI
Sbjct: 243 YRSTFSYCLPSFKSVNFSGSLRLGPVAQPIRIKYTPLLRNPRRSSLYYVNLIAIRVGRRI 302

Query: 302 VDIPPSALAFNSVTGAGTIIDSGTTFTRLVAPAYTAVRDEFRRRVGRNITVSSLGGFDTC 361
           VDIPPSALAFNS TGAGTIIDSGTTFTRLVAPAYTAVRDEFRRRVGRN+TVSSLGGFDTC
Sbjct: 303 VDIPPSALAFNSATGAGTIIDSGTTFTRLVAPAYTAVRDEFRRRVGRNVTVSSLGGFDTC 362

Query: 362 YTVPIISPTITLMFAGMNVTLPQDNFLIHSTAGSTTCLAMAAAPDNVNSVLNVIASMQQQ 421
           YTVPIISPTIT MFAGMNVTLP DNFLIHSTAGSTTCLAMAAAPDNVNSVLNVIASMQQQ
Sbjct: 363 YTVPIISPTITFMFAGMNVTLPPDNFLIHSTAGSTTCLAMAAAPDNVNSVLNVIASMQQQ 422

Query: 422 NHRILFDIPNSRLGVAREPCSS 441
           NHRILFDIPNSR+GVAREPCSS
Sbjct: 423 NHRILFDIPNSRVGVAREPCSS 444

[Close]

BLAST of Lsi04G000230 vs. NCBI nr
Match: XP_004142420.1 (aspartyl protease AED3 [Cucumis sativus] >KGN52324.1 hypothetical protein Csa_008233 [Cucumis sativus])

HSP 1 Score: 801.2 bits (2068), Expect = 4.5e-228
Identity = 409/440 (92.95%), Postives = 421/440 (95.68%), Query Frame = 0


Query: 2   NTKSPFLLFLLFLLLSVSAESLAAHTP-NCNAAADRSSTLQVFHIFSPCSPFRPSKPLSW 61
           NTKSPFLL LL L L  S  +  +H P NCN AADRSSTLQVFHIFSPCSPFRPSKPLSW
Sbjct: 3   NTKSPFLLLLLLLHL-FSISTAKSHIPSNCNPAADRSSTLQVFHIFSPCSPFRPSKPLSW 62

Query: 62  ADNVLQMQAKDQARVQFLSSLVARRSVVPIASARQLIQSPTFVVRAKIGTPAQTLLLALD 121
           ADNVLQMQAKDQAR+QFLSSLVARRS VPIASARQLIQSPTFVVRAKIGTPAQTLLLALD
Sbjct: 63  ADNVLQMQAKDQARLQFLSSLVARRSFVPIASARQLIQSPTFVVRAKIGTPAQTLLLALD 122

Query: 122 TSNDAAWIPCSGCVGCPSATVFSSDKSSSFRPLPCQSPQCNQVPNPSCTGSACGFNLTYG 181
           TSNDAAWIPCSGC+GCPS TVFSSDKSSSFRPLPCQSPQCNQVPNPSC+GSACGFNLTYG
Sbjct: 123 TSNDAAWIPCSGCIGCPSTTVFSSDKSSSFRPLPCQSPQCNQVPNPSCSGSACGFNLTYG 182

Query: 182 SSTVAADLVQDNITLATDSVPAYTFGCITKATGSSVPPQGLLGLGRGPLSLLGQSQSLYQ 241
           SSTVAADLVQDN+TLATDSVP+YTFGCI KATGSSVPPQGLLGLGRGPLSLLGQSQSLYQ
Sbjct: 183 SSTVAADLVQDNLTLATDSVPSYTFGCIRKATGSSVPPQGLLGLGRGPLSLLGQSQSLYQ 242

Query: 242 STFSYCLPSFKSVNFSGSLRLGPVAQPIRIKYTPLLRNPRRSSLYYVNLIAIRVGRRIVD 301
           STFSYCLPSFKSVNFSGSLRLGPVAQPIRIKYTPLLRNPRRSSLYYVNLI+IRVGR+IVD
Sbjct: 243 STFSYCLPSFKSVNFSGSLRLGPVAQPIRIKYTPLLRNPRRSSLYYVNLISIRVGRKIVD 302

Query: 302 IPPSALAFNSVTGAGTIIDSGTTFTRLVAPAYTAVRDEFRRRVGRNITVSSLGGFDTCYT 361
           IPPSALAFNS TGAGT+IDSGTTFTRLVAPAYTAVRDEFRRRVGRN+TVSSLGGFDTCYT
Sbjct: 303 IPPSALAFNSATGAGTVIDSGTTFTRLVAPAYTAVRDEFRRRVGRNVTVSSLGGFDTCYT 362

Query: 362 VPIISPTITLMFAGMNVTLPQDNFLIHSTAGSTTCLAMAAAPDNVNSVLNVIASMQQQNH 421
           VPIISPTIT MFAGMNVTLP DNFLIHSTAGSTTCLAMAAAPDNVNSVLNVIASMQQQNH
Sbjct: 363 VPIISPTITFMFAGMNVTLPPDNFLIHSTAGSTTCLAMAAAPDNVNSVLNVIASMQQQNH 422

Query: 422 RILFDIPNSRLGVAREPCSS 441
           RILFDIPNSR+GVARE CSS
Sbjct: 423 RILFDIPNSRVGVARESCSS 441

[Close]

BLAST of Lsi04G000230 vs. NCBI nr
Match: XP_022957241.1 (aspartyl protease AED3 [Cucurbita moschata])

HSP 1 Score: 793.5 bits (2048), Expect = 9.3e-226
Identity = 405/439 (92.26%), Postives = 420/439 (95.67%), Query Frame = 0


Query: 1   MNTKSPFLLFLLFLLLSVSAESLAAHTPNCNAAADRSSTLQVFHIFSPCSPFRPSKPLSW 60
           M TKSPFLL LL LLLS+SAES   H PNCNAAADRSSTLQVFHIFSPCSPFRPSKPLSW
Sbjct: 1   MKTKSPFLLSLL-LLLSISAESHHHHHPNCNAAADRSSTLQVFHIFSPCSPFRPSKPLSW 60

Query: 61  ADNVLQMQAKDQARVQFLSSLVARRSVVPIASARQLIQSPTFVVRAKIGTPAQTLLLALD 120
           ADNVLQMQAKDQAR+QFLSSLVAR+SV PIASARQLIQSPTFVVRAKIGTPAQTLL+ALD
Sbjct: 61  ADNVLQMQAKDQARLQFLSSLVARKSVAPIASARQLIQSPTFVVRAKIGTPAQTLLMALD 120

Query: 121 TSNDAAWIPCSGCVGCPSATVFSSDKSSSFRPLPCQSPQCNQVPNPSCTGSACGFNLTYG 180
           TSNDAAWIPCSGCVGCPS TVFSSDKSSSFRPLPC+SPQCNQVPNPSC+ SACGFNLTYG
Sbjct: 121 TSNDAAWIPCSGCVGCPSTTVFSSDKSSSFRPLPCRSPQCNQVPNPSCSSSACGFNLTYG 180

Query: 181 SSTVAADLVQDNITLATDSVPAYTFGCITKATGSSVPPQGLLGLGRGPLSLLGQSQSLYQ 240
           SSTVAADLVQDNITLA DSVPAYTFGCITKATGSSVPPQGLLGLGRGPLSLLGQSQSLY+
Sbjct: 181 SSTVAADLVQDNITLANDSVPAYTFGCITKATGSSVPPQGLLGLGRGPLSLLGQSQSLYR 240

Query: 241 STFSYCLPSFKSVNFSGSLRLGPVAQPIRIKYTPLLRNPRRSSLYYVNLIAIRVGRRIVD 300
           STFSYCLPSFKS  FSGSLRLGPVAQP RIKYTPLLRNPRRSSLYYVNLIAIRVGRRIVD
Sbjct: 241 STFSYCLPSFKSTGFSGSLRLGPVAQPKRIKYTPLLRNPRRSSLYYVNLIAIRVGRRIVD 300

Query: 301 IPPSALAFNSVTGAGTIIDSGTTFTRLVAPAYTAVRDEFRRRVGRNITVSSLGGFDTCYT 360
           IPPSALAFNS TGAGTIIDSGTTFTRLVAPAYTAVR+EFRRR+G N T+SSLGGFDTCYT
Sbjct: 301 IPPSALAFNSATGAGTIIDSGTTFTRLVAPAYTAVRNEFRRRIG-NATISSLGGFDTCYT 360

Query: 361 VPIISPTITLMFAGMNVTLPQDNFLIHSTAGSTTCLAMAAAPDNVNSVLNVIASMQQQNH 420
           VPIISPTIT MFAGMNVTLP DNFL+HS+AG+TTCLAMAAAPDNVNSVLNVIASMQQQNH
Sbjct: 361 VPIISPTITFMFAGMNVTLPPDNFLLHSSAGTTTCLAMAAAPDNVNSVLNVIASMQQQNH 420

Query: 421 RILFDIPNSRLGVAREPCS 440
           RILFD+PNSR+GVAREPCS
Sbjct: 421 RILFDVPNSRIGVAREPCS 437

[Close]

BLAST of Lsi04G000230 vs. NCBI nr
Match: XP_022988941.1 (aspartyl protease AED3-like [Cucurbita maxima])

HSP 1 Score: 792.3 bits (2045), Expect = 2.1e-225
Identity = 404/440 (91.82%), Postives = 421/440 (95.68%), Query Frame = 0


Query: 1   MNTKSPFLLFLLFLLLSVSAESL-AAHTPNCNAAADRSSTLQVFHIFSPCSPFRPSKPLS 60
           M TKSPFLL LL LLLS+SAESL   H PNCNAAADRSSTLQVFHIFSPCSPFRPSKPLS
Sbjct: 1   MKTKSPFLLSLLLLLLSISAESLHHHHHPNCNAAADRSSTLQVFHIFSPCSPFRPSKPLS 60

Query: 61  WADNVLQMQAKDQARVQFLSSLVARRSVVPIASARQLIQSPTFVVRAKIGTPAQTLLLAL 120
           WADNVLQMQAKDQAR+QFLSSLVA +SV PIASARQLIQSPTFVVRAKIGTPAQTLL+AL
Sbjct: 61  WADNVLQMQAKDQARLQFLSSLVACKSVAPIASARQLIQSPTFVVRAKIGTPAQTLLMAL 120

Query: 121 DTSNDAAWIPCSGCVGCPSATVFSSDKSSSFRPLPCQSPQCNQVPNPSCTGSACGFNLTY 180
           DTSNDAAWIPCSGCVGCPS TVFSSDKS+SFRPLPC+SPQCNQVPNPSC+ SACGFNLTY
Sbjct: 121 DTSNDAAWIPCSGCVGCPSTTVFSSDKSTSFRPLPCRSPQCNQVPNPSCSSSACGFNLTY 180

Query: 181 GSSTVAADLVQDNITLATDSVPAYTFGCITKATGSSVPPQGLLGLGRGPLSLLGQSQSLY 240
           GSSTVAADLVQDNITLA DSVPAYTFGCITKATGSSVPPQGLLGLGRGPLSLLGQSQSLY
Sbjct: 181 GSSTVAADLVQDNITLANDSVPAYTFGCITKATGSSVPPQGLLGLGRGPLSLLGQSQSLY 240

Query: 241 QSTFSYCLPSFKSVNFSGSLRLGPVAQPIRIKYTPLLRNPRRSSLYYVNLIAIRVGRRIV 300
           +STFSYCLPSFKS +FSGSLRLGPVAQP RIKYTPLLRNPRRSSLYYVNLIAIRVGRRIV
Sbjct: 241 RSTFSYCLPSFKSTSFSGSLRLGPVAQPKRIKYTPLLRNPRRSSLYYVNLIAIRVGRRIV 300

Query: 301 DIPPSALAFNSVTGAGTIIDSGTTFTRLVAPAYTAVRDEFRRRVGRNITVSSLGGFDTCY 360
           DIPPSALAFNS TGAGTIIDSGTTFTRLVAPAYTAVR+EFRRR+G N T+SSLGGFDTCY
Sbjct: 301 DIPPSALAFNSATGAGTIIDSGTTFTRLVAPAYTAVRNEFRRRIG-NATISSLGGFDTCY 360

Query: 361 TVPIISPTITLMFAGMNVTLPQDNFLIHSTAGSTTCLAMAAAPDNVNSVLNVIASMQQQN 420
           TVPIISPTIT MFAGMNVTLP DNFL+HS+AG+TTCLAMAAAPDNVNSVLNVIASMQQQN
Sbjct: 361 TVPIISPTITFMFAGMNVTLPPDNFLLHSSAGTTTCLAMAAAPDNVNSVLNVIASMQQQN 420

Query: 421 HRILFDIPNSRLGVAREPCS 440
           HRILFD+PNSR+GVAREPCS
Sbjct: 421 HRILFDVPNSRMGVAREPCS 439

[Close]

BLAST of Lsi04G000230 vs. TAIR 10
Match: AT5G07030.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 584.3 bits (1505), Expect = 8.0e-167
Identity = 293/443 (66.14%), Postives = 354/443 (79.91%), Query Frame = 0


Query: 2   NTKSPFLLFLLFLLLSVSAESLAAHTPNCN--AAADRSSTLQVFHIFSPCSPFRPSKPLS 61
           NT S  +LFL   L S+   +L  + PNC+     D+ STL++FHI SPCSPF+ S PLS
Sbjct: 15  NTMSTLVLFL--QLFSILPLALGLNHPNCDLTKTQDQGSTLRIFHIDSPCSPFKSSSPLS 74

Query: 62  WADNVLQMQAKDQARVQFLSSLVARRSVVPIASARQLIQSPTFVVRAKIGTPAQTLLLAL 121
           W   VLQ  A+DQAR+Q+LSSLVA RSVVPIAS RQ++QS T++V+A IGTPAQ LLLA+
Sbjct: 75  WEARVLQTLAQDQARLQYLSSLVAGRSVVPIASGRQMLQSTTYIVKALIGTPAQPLLLAM 134

Query: 122 DTSNDAAWIPCSGCVGCPSATVFSSDKSSSFRPLPCQSPQCNQVPNPSCTGSACGFNLTY 181
           DTS+D AWIPCSGCVGCPS T FS  KS+SF+ + C +PQC QVPNP+C   AC FNLTY
Sbjct: 135 DTSSDVAWIPCSGCVGCPSNTAFSPAKSTSFKNVSCSAPQCKQVPNPTCGARACSFNLTY 194

Query: 182 GSSTVAADLVQDNITLATDSVPAYTFGCITKATGSSV--PPQGLLGLGRGPLSLLGQSQS 241
           GSS++AA+L QD I LA D + A+TFGC+ K  G     PPQGLLGLGRGPLSL+ Q+QS
Sbjct: 195 GSSSIAANLSQDTIRLAADPIKAFTFGCVNKVAGGGTIPPPQGLLGLGRGPLSLMSQAQS 254

Query: 242 LYQSTFSYCLPSFKSVNFSGSLRLGPVAQPIRIKYTPLLRNPRRSSLYYVNLIAIRVGRR 301
           +Y+STFSYCLPSF+S+ FSGSLRLGP +QP R+KYT LLRNPRRSSLYYVNL+AIRVGR+
Sbjct: 255 IYKSTFSYCLPSFRSLTFSGSLRLGPTSQPQRVKYTQLLRNPRRSSLYYVNLVAIRVGRK 314

Query: 302 IVDIPPSALAFNSVTGAGTIIDSGTTFTRLVAPAYTAVRDEFRRRV-GRNITVSSLGGFD 361
           +VD+PP+A+AFN  TGAGTI DSGT +TRL  P Y AVR+EFR+RV      V+SLGGFD
Sbjct: 315 VVDLPPAAIAFNPSTGAGTIFDSGTVYTRLAKPVYEAVRNEFRKRVKPTTAVVTSLGGFD 374

Query: 362 TCYTVPIISPTITLMFAGMNVTLPQDNFLIHSTAGSTTCLAMAAAPDNVNSVLNVIASMQ 421
           TCY+  +  PTIT MF G+N+T+P DN ++HSTAGST+CLAMAAAP+NVNSV+NVIASMQ
Sbjct: 375 TCYSGQVKVPTITFMFKGVNMTMPADNLMLHSTAGSTSCLAMAAAPENVNSVVNVIASMQ 434

Query: 422 QQNHRILFDIPNSRLGVAREPCS 440
           QQNHR+L D+PN RLG+ARE CS
Sbjct: 435 QQNHRVLIDVPNGRLGLARERCS 455

[Close]

BLAST of Lsi04G000230 vs. TAIR 10
Match: AT3G54400.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 562.8 bits (1449), Expect = 2.5e-160
Identity = 294/434 (67.74%), Postives = 349/434 (80.41%), Query Frame = 0


Query: 8   LLFLLFLLLSVSAESLAAHTPNCNAAADRSSTLQVFHIFSPCSPFRPSKPLSWADNVLQM 67
           LL LL  LL + +ES+     NCN  +  SS L+VFHI S CSPF+ S  +SWAD +LQ 
Sbjct: 5   LLILLISLLILKSESI-----NCNEKS-HSSDLRVFHINSLCSPFKTS--VSWADTLLQ- 64

Query: 68  QAKDQARVQFLSSLV-ARRSVVPIASARQLIQSPTFVVRAKIGTPAQTLLLALDTSNDAA 127
              D+AR  +LSSL   R+S VPIAS R ++QSPT++VRA IGTPAQ +L+ALDTSNDAA
Sbjct: 65  ---DKARFLYLSSLAGVRKSSVPIASGRAIVQSPTYIVRANIGTPAQPMLVALDTSNDAA 124

Query: 128 WIPCSGCVGCPSATVFSSDKSSSFRPLPCQSPQCNQVPNPSCTGS-ACGFNLTYGSSTVA 187
           WIPCSGCVGC S+ +F   KSSS R L C++PQC Q PNPSCT S +CGFN+TYG ST+ 
Sbjct: 125 WIPCSGCVGCSSSVLFDPSKSSSSRTLQCEAPQCKQAPNPSCTVSKSCGFNMTYGGSTIE 184

Query: 188 ADLVQDNITLATDSVPAYTFGCITKATGSSVPPQGLLGLGRGPLSLLGQSQSLYQSTFSY 247
           A L QD +TLA+D +P YTFGCI KA+G+S+P QGL+GLGRGPLSL+ QSQ+LYQSTFSY
Sbjct: 185 AYLTQDTLTLASDVIPNYTFGCINKASGTSLPAQGLMGLGRGPLSLISQSQNLYQSTFSY 244

Query: 248 CLPSFKSVNFSGSLRLGPVAQPIRIKYTPLLRNPRRSSLYYVNLIAIRVGRRIVDIPPSA 307
           CLP+ KS NFSGSLRLGP  QPIRIK TPLL+NPRRSSLYYVNL+ IRVG +IVDIP SA
Sbjct: 245 CLPNSKSSNFSGSLRLGPKNQPIRIKTTPLLKNPRRSSLYYVNLVGIRVGNKIVDIPTSA 304

Query: 308 LAFNSVTGAGTIIDSGTTFTRLVAPAYTAVRDEFRRRVGRNITVSSLGGFDTCYTVPIIS 367
           LAF+  TGAGTI DSGT +TRLV PAY AVR+EFRRRV +N   +SLGGFDTCY+  ++ 
Sbjct: 305 LAFDPATGAGTIFDSGTVYTRLVEPAYVAVRNEFRRRV-KNANATSLGGFDTCYSGSVVF 364

Query: 368 PTITLMFAGMNVTLPQDNFLIHSTAGSTTCLAMAAAPDNVNSVLNVIASMQQQNHRILFD 427
           P++T MFAGMNVTLP DN LIHS+AG+ +CLAMAAAP NVNSVLNVIASMQQQNHR+L D
Sbjct: 365 PSVTFMFAGMNVTLPPDNLLIHSSAGNLSCLAMAAAPVNVNSVLNVIASMQQQNHRVLID 424

Query: 428 IPNSRLGVAREPCS 440
           +PNSRLG++RE C+
Sbjct: 425 VPNSRLGISRETCT 425

[Close]

BLAST of Lsi04G000230 vs. TAIR 10
Match: AT1G09750.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 409.1 bits (1050), Expect = 4.6e-114
Identity = 228/449 (50.78%), Postives = 299/449 (66.59%), Query Frame = 0


Query: 5   SPFLLFLLFLLLSVSAESLAAHTPNC-NAAADRSSTLQVFHIFSPCSPFRPSK-PLSWAD 64
           S  L F  FL L +      A    C  AA D S  L +  I + CSPF P+    S  D
Sbjct: 3   SSSLHFFFFLTLLLPFTFTTATRDTCATAAPDGSDDLSIIPINAKCSPFAPTHVSASVID 62

Query: 65  NVLQMQAKDQARVQFLSSLVA---RRSVVPIASARQLIQSPTFVVRAKIGTPAQTLLLAL 124
            VL M + D  R+ +LSSLVA   + + VP+AS  QL     +VVRAK+GTP Q + + L
Sbjct: 63  TVLHMASSDSHRLTYLSSLVAGKPKPTSVPVASGNQL-HIGNYVVRAKLGTPPQLMFMVL 122

Query: 125 DTSNDAAWIPCSGCVGCPSA-TVFSSDKSSSFRPLPCQSPQCNQV-----PNPSCTGSAC 184
           DTSNDA W+PCSGC GC +A T F+++ SS++  + C + QC Q      P+ S   S C
Sbjct: 123 DTSNDAVWLPCSGCSGCSNASTSFNTNSSSTYSTVSCSTAQCTQARGLTCPSSSPQPSVC 182

Query: 185 GFNLTY-GSSTVAADLVQDNITLATDSVPAYTFGCITKATGSSVPPQGLLGLGRGPLSLL 244
            FN +Y G S+ +A LVQD +TLA D +P ++FGCI  A+G+S+PPQGL+GLGRGP+SL+
Sbjct: 183 SFNQSYGGDSSFSASLVQDTLTLAPDVIPNFSFGCINSASGNSLPPQGLMGLGRGPMSLV 242

Query: 245 GQSQSLYQSTFSYCLPSFKSVNFSGSLRLGPVAQPIRIKYTPLLRNPRRSSLYYVNLIAI 304
            Q+ SLY   FSYCLPSF+S  FSGSL+LG + QP  I+YTPLLRNPRR SLYYVNL  +
Sbjct: 243 SQTTSLYSGVFSYCLPSFRSFYFSGSLKLGLLGQPKSIRYTPLLRNPRRPSLYYVNLTGV 302

Query: 305 RVGRRIVDIPPSALAFNSVTGAGTIIDSGTTFTRLVAPAYTAVRDEFRRRVGRNITVSSL 364
            VG   V + P  L F++ +GAGTIIDSGT  TR   P Y A+RDEFR++V  + + S+L
Sbjct: 303 SVGSVQVPVDPVYLTFDANSGAGTIIDSGTVITRFAQPVYEAIRDEFRKQVNVS-SFSTL 362

Query: 365 GGFDTCYTV--PIISPTITLMFAGMNVTLPQDNFLIHSTAGSTTCLAMAAAPDNVNSVLN 424
           G FDTC++     ++P ITL    +++ LP +N LIHS+AG+ TCL+MA    N N+VLN
Sbjct: 363 GAFDTCFSADNENVAPKITLHMTSLDLKLPMENTLIHSSAGTLTCLSMAGIRQNANAVLN 422

Query: 425 VIASMQQQNHRILFDIPNSRLGVAREPCS 440
           VIA++QQQN RILFD+PNSR+G+A EPC+
Sbjct: 423 VIANLQQQNLRILFDVPNSRIGIAPEPCN 449

[Close]

BLAST of Lsi04G000230 vs. TAIR 10
Match: AT1G01300.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 192.2 bits (487), Expect = 8.8e-49
Identity = 117/351 (33.33%), Postives = 176/351 (50.14%), Query Frame = 0


Query: 99  SPTFVVRAKIGTPAQTLLLALDTSNDAAWIPCSGCVGCPSAT--VFSSDKSSSFRPLPCQ 158
           S  +  R  +GTPA+ + + LDT +D  W+ C+ C  C S +  +F   KS ++  +PC 
Sbjct: 139 SGEYFTRLGVGTPARYVYMVLDTGSDIVWLQCAPCRRCYSQSDPIFDPRKSKTYATIPCS 198

Query: 159 SPQCNQVPNPSCT--GSACGFNLTYG-SSTVAADLVQDNITLATDSVPAYTFGCITKATG 218
           SP C ++ +  C      C + ++YG  S    D   + +T   + V     GC     G
Sbjct: 199 SPHCRRLDSAGCNTRRKTCLYQVSYGDGSFTVGDFSTETLTFRRNRVKGVALGCGHDNEG 258

Query: 219 SSVPPQGLLGLGRGPLSLLGQSQSLYQSTFSYCLPSFKSVNFSGSLRLGPVAQPIRIKYT 278
             V   GLLGLG+G LS  GQ+   +   FSYCL    + +   S+  G  A     ++T
Sbjct: 259 LFVGAAGLLGLGKGKLSFPGQTGHRFNQKFSYCLVDRSASSKPSSVVFGNAAVSRIARFT 318

Query: 279 PLLRNPRRSSLYYVNLIAIRV-GRRIVDIPPSALAFNSVTGAGTIIDSGTTFTRLVAPAY 338
           PLL NP+  + YYV L+ I V G R+  +  S    + +   G IIDSGT+ TRL+ PAY
Sbjct: 319 PLLSNPKLDTFYYVGLLGISVGGTRVPGVTASLFKLDQIGNGGVIIDSGTSVTRLIRPAY 378

Query: 339 TAVRDEFRRRVGRNITVSSLGGFDTCYTVPIIS----PTITLMFAGMNVTLPQDNFLIHS 398
            A+RD FR              FDTC+ +  ++    PT+ L F G +V+LP  N+LI  
Sbjct: 379 IAMRDAFRVGAKTLKRAPDFSLFDTCFDLSNMNEVKVPTVVLHFRGADVSLPATNYLIPV 438

Query: 399 TAGSTTCLAMAAAPDNVNSVLNVIASMQQQNHRILFDIPNSRLGVAREPCS 440
                 C A A         L++I ++QQQ  R+++D+ +SR+G A   C+
Sbjct: 439 DTNGKFCFAFAGTMGG----LSIIGNIQQQGFRVVYDLASSRVGFAPGGCA 485

[Close]

BLAST of Lsi04G000230 vs. TAIR 10
Match: AT3G61820.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 179.1 bits (453), Expect = 7.7e-45
Identity = 142/454 (31.28%), Postives = 206/454 (45.37%), Query Frame = 0


Query: 17  SVSAESLAAHTPNCNAAADRSSTLQVFHIFSPCSPFRPSKPLSWADNVLQMQAKDQARVQ 76
           S++ ESL+  T + +        L  F   SP   F            L++Q +D  RV+
Sbjct: 48  SLTDESLSESTTSLSVHLSHVDALSSFSDASPADLFN-----------LRLQ-RDSLRVK 107

Query: 77  FLSSLVA--------RR--------SVVPIASARQLIQSPTFVVRAKIGTPAQTLLLALD 136
            ++SL A        +R        S   I+   Q   S  + +R  +GTPA  + + LD
Sbjct: 108 SITSLAAVSTGRNATKRTPRTAGGFSGAVISGLSQ--GSGEYFMRLGVGTPATNVYMVLD 167

Query: 137 TSNDAAWIPCSGCVGCPSAT--VFSSDKSSSFRPLPCQSPQCNQVPNPS-CT---GSACG 196
           T +D  W+ CS C  C + T  +F   KS +F  +PC S  C ++ + S C       C 
Sbjct: 168 TGSDVVWLQCSPCKACYNQTDAIFDPKKSKTFATVPCGSRLCRRLDDSSECVTRRSKTCL 227

Query: 197 FNLTYG-SSTVAADLVQDNITLATDSVPAYTFGCITKATGSSVPPQGLLGLGRGPLSLLG 256
           + ++YG  S    D   + +T     V     GC     G  V   GLLGLGRG LS   
Sbjct: 228 YQVSYGDGSFTEGDFSTETLTFHGARVDHVPLGCGHDNEGLFVGAAGLLGLGRGGLSFPS 287

Query: 257 QSQSLYQSTFSYCLPSFKSVNFS----GSLRLGPVAQPIRIKYTPLLRNPRRSSLYYVNL 316
           Q+++ Y   FSYCL    S   S     ++  G  A P    +TPLL NP+  + YY+ L
Sbjct: 288 QTKNRYNGKFSYCLVDRTSSGSSSKPPSTIVFGNAAVPKTSVFTPLLTNPKLDTFYYLQL 347

Query: 317 IAIRV-GRRIVDIPPSALAFNSVTGAGTIIDSGTTFTRLVAPAYTAVRDEFRRRVGRNIT 376
           + I V G R+  +  S    ++    G IIDSGT+ TRL  PAY A+RD FR    +   
Sbjct: 348 LGISVGGSRVPGVSESQFKLDATGNGGVIIDSGTSVTRLTQPAYVALRDAFRLGATKLKR 407

Query: 377 VSSLGGFDTCYTV----PIISPTITLMFAGMNVTLPQDNFLIHSTAGSTTCLAMAAAPDN 436
             S   FDTC+ +     +  PT+   F G  V+LP  N+LI        C A A    +
Sbjct: 408 APSYSLFDTCFDLSGMTTVKVPTVVFHFGGGEVSLPASNYLIPVNTEGRFCFAFAGTMGS 467

Query: 437 VNSVLNVIASMQQQNHRILFDIPNSRLGVAREPC 439
               L++I ++QQQ  R+ +D+  SR+G     C
Sbjct: 468 ----LSIIGNIQQQGFRVAYDLVGSRVGFLSRAC 483

The following BLAST results are available for this feature:

BLAST of Lsi04G000230 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O04496	6.5e-113	50.78	Aspartyl protease AED3 OS=Arabidopsis thaliana OX=3702 GN=AED3 PE=1 SV=1	[more]
Q6F4N5	1.1e-96	45.47	Aspartyl protease 25 OS=Oryza sativa subsp. japonica OX=39947 GN=AP25 PE=2 SV=1	[more]
Q9LNJ3	1.2e-47	33.33	Aspartyl protease family protein 2 OS=Arabidopsis thaliana OX=3702 GN=APF2 PE=2 ...	[more]
Q9LHE3	7.1e-43	32.18	Protein ASPARTIC PROTEASE IN GUARD CELL 2 OS=Arabidopsis thaliana OX=3702 GN=ASP...	[more]
Q9LEW3	2.1e-42	30.93	Aspartyl protease AED1 OS=Arabidopsis thaliana OX=3702 GN=AED1 PE=2 SV=1	[more]

BLAST of Lsi04G000230 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7SU62	5.7e-229	93.21	Aspartyl protease AED3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold4...	[more]
A0A1S3BGB2	5.7e-229	93.21	aspartyl protease AED3 OS=Cucumis melo OX=3656 GN=LOC103489519 PE=4 SV=1	[more]
A0A0A0KTR5	2.2e-228	92.95	Aspartic proteinase nepenthesin-1 OS=Cucumis sativus OX=3659 GN=Csa_5G623870 PE=...	[more]
A0A6J1GYP2	4.5e-226	92.26	aspartyl protease AED3 OS=Cucurbita moschata OX=3662 GN=LOC111458686 PE=4 SV=1	[more]
A0A6J1JIM3	1.0e-225	91.82	aspartyl protease AED3-like OS=Cucurbita maxima OX=3661 GN=LOC111486144 PE=4 SV=...	[more]

BLAST of Lsi04G000230 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038892592.1	3.5e-233	95.46	aspartyl protease AED3 [Benincasa hispida]	[more]
XP_008446966.1	1.2e-228	93.21	PREDICTED: aspartyl protease AED3 [Cucumis melo] >KAA0034764.1 aspartyl protease...	[more]
XP_004142420.1	4.5e-228	92.95	aspartyl protease AED3 [Cucumis sativus] >KGN52324.1 hypothetical protein Csa_00...	[more]
XP_022957241.1	9.3e-226	92.26	aspartyl protease AED3 [Cucurbita moschata]	[more]
XP_022988941.1	2.1e-225	91.82	aspartyl protease AED3-like [Cucurbita maxima]	[more]

BLAST of Lsi04G000230 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G07030.1	8.0e-167	66.14	Eukaryotic aspartyl protease family protein	[more]
AT3G54400.1	2.5e-160	67.74	Eukaryotic aspartyl protease family protein	[more]
AT1G09750.1	4.6e-114	50.78	Eukaryotic aspartyl protease family protein	[more]
AT1G01300.1	8.8e-49	33.33	Eukaryotic aspartyl protease family protein	[more]
AT3G61820.1	7.7e-45	31.28	Eukaryotic aspartyl protease family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR032799	Xylanase inhibitor, C-terminal	PFAM	PF14541	TAXi_C	coord: 285..432 e-value: 3.7E-29 score: 101.6
IPR032861	Xylanase inhibitor, N-terminal	PFAM	PF14543	TAXi_N	coord: 102..262 e-value: 1.5E-34 score: 119.8
IPR021109	Aspartic peptidase domain superfamily	GENE3D	2.40.70.10	Acid Proteases	coord: 79..262 e-value: 3.7E-36 score: 126.9
IPR021109	Aspartic peptidase domain superfamily	GENE3D	2.40.70.10	Acid Proteases	coord: 264..440 e-value: 7.4E-46 score: 158.1
IPR021109	Aspartic peptidase domain superfamily	SUPERFAMILY	50630	Acid proteases	coord: 101..438
IPR001461	Aspartic peptidase A1 family	PANTHER	PTHR13683	ASPARTYL PROTEASES	coord: 8..439
None	No IPR available	PANTHER	PTHR13683:SF798	ASPARTYL PROTEASE AED3-LIKE	coord: 8..439
IPR033121	Peptidase family A1 domain	PROSITE	PS51767	PEPTIDASE_A1	coord: 102..434 score: 37.355083

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi04G000230.1	Lsi04G000230.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006508	proteolysis
molecular_function	GO:0004190	aspartic-type endopeptidase activity