Lsi03G022720 (gene) Bottle gourd (USVL1VR-Ls) v1

BLAST of Lsi03G022720 vs. ExPASy Swiss-Prot
Match: Q9ZVH5 (Protein DETOXIFICATION 53 OS=Arabidopsis thaliana OX=3702 GN=DTX53 PE=2 SV=1)

HSP 1 Score: 612.5 bits (1578), Expect = 5.0e-174
Identity = 298/469 (63.54%), Postives = 381/469 (81.24%), Query Frame = 0

Query: 98  QMKEELKSLARFAGPIIMTSFLIYSRSVVSMLFLGHLGKAELAGGSLALGFGNITGISIL 157
           Q+ EE+ SL + A PI+MTS LI+SRS++SM FL HLGK ELAGG+LA+GFGNITG+S+L
Sbjct: 2   QVGEEMASLTKIACPIVMTSLLIFSRSIISMWFLSHLGKVELAGGALAMGFGNITGVSVL 61

Query: 158 RGLSTGMDPICCQAFGAKRWSVLSQTFLKTLCLLLLVSIPISILWLNMEPILLWLGQDPA 217
           +GLS GMDPIC QAFGAKRW+VLS TF K  CLL++VS+PI++ WLN+EPI L LGQDP 
Sbjct: 62  KGLSVGMDPICGQAFGAKRWTVLSHTFQKMFCLLIVVSVPIAVTWLNIEPIFLRLGQDPD 121

Query: 218 ITQVAKVYMVFSIPELLAQAHHLPLRIFLRTQGITTPITVASVCSALLHPPINYFLVTYL 277
           IT+VAK YM+F +PELLAQA   PLR FLRTQG+T+P+T++++ S LLHP  NY  V  +
Sbjct: 122 ITKVAKTYMLFFVPELLAQAMLHPLRTFLRTQGLTSPLTISAIVSILLHPLFNYVFVVRM 181

Query: 278 KLGVEGVALSLAWNTLNLNVGLMIYLALSSKPLKPWHGVTILSTFQGWQPLLSLAVPSAV 337
           +LGV+GVA+++A+NT+N++VGL++Y   S   +KPW G+ + S F+GW PLLSLA PSA+
Sbjct: 182 RLGVKGVAIAMAFNTMNIDVGLLVYTCFSDSLIKPWEGLALRSLFRGWWPLLSLAAPSAI 241

Query: 338 SVCLEWWWYEIMLFLCGLLNHPQNTVAAMGILIQTTGMLYIVPFSLSAGITTRIGHALGA 397
           SVCLE+WWYEIMLFLCGLL +P+ +VAAMGILIQTTG+LY+VPF++S+ I TR+GHALG 
Sbjct: 242 SVCLEYWWYEIMLFLCGLLGNPKASVAAMGILIQTTGILYVVPFAISSAIATRVGHALGG 301

Query: 398 GEPIRAQWTAVIGLSTGLAFGLTAFLFMTSVRSVWGKLYTDEPEILRMISSALPILGLCE 457
           G+P RAQ T VIGL   +A+GL A +F+T++RSVWGK++TDEPEIL +IS+ALPILGLCE
Sbjct: 302 GQPTRAQCTTVIGLILAVAYGLAAAVFVTALRSVWGKMFTDEPEILGLISAALPILGLCE 361

Query: 458 ISNSPQTVACGVLTGTARPKLGARINLYAFYFIGLPVAVLATFTLKTGFLGLWFGLMTAQ 517
           I NSPQT ACGVLTGTARPK GAR+NL AFY +GLPVAV  TF  K GF GLWFGL++AQ
Sbjct: 362 IGNSPQTAACGVLTGTARPKDGARVNLCAFYIVGLPVAVTTTFGFKVGFRGLWFGLLSAQ 421

Query: 518 ISCLCMLVRTLLRTDWIQQSARAVELAATVGEEIAKEEEDVESGLIDDN 567
           ++CL M++ TL+RTDW  Q  RA EL +   ++   E+E V + + DD+
Sbjct: 422 MTCLVMMLYTLIRTDWSHQVKRAEELTSAAADKSHSEDETVHAEVQDDD 470

BLAST of Lsi03G022720 vs. ExPASy Swiss-Prot
Match: Q9SLV0 (Protein DETOXIFICATION 48 OS=Arabidopsis thaliana OX=3702 GN=DTX48 PE=2 SV=1)

HSP 1 Score: 465.7 bits (1197), Expect = 7.5e-130
Identity = 236/445 (53.03%), Postives = 318/445 (71.46%), Query Frame = 0

Query: 101 EELKSLARFAGPIIMTSFLIYSRSVVSMLFLGHLGKAELAGGSLALGFGNITGISILRGL 160
           EE+K++ + +GP  MT  L+YSR+++SMLFLG+LG+ ELAGGSL++GF NITG S++ GL
Sbjct: 57  EEVKAIGKISGPTAMTGLLMYSRAMISMLFLGYLGELELAGGSLSIGFANITGYSVISGL 116

Query: 161 STGMDPICCQAFGAKRWSVLSQTFLKTLCLLLLVSIPISILWLNMEPILLWLGQDPAITQ 220
           S GM+PIC QA+GAK+  +L  T  +T+ LLL  S+PIS  WLNM  ILLW GQD  I+ 
Sbjct: 117 SMGMEPICGQAYGAKQMKLLGLTLQRTVLLLLSCSVPISFSWLNMRRILLWCGQDEEISS 176

Query: 221 VAKVYMVFSIPELLAQAHHLPLRIFLRTQGITTPITVASVCSALLHPPINYFLVTYLKLG 280
           VA+ +++F+IP+L   +   PLRI+LRTQ IT P+T ++  S LLH P+NY LV  L++G
Sbjct: 177 VAQQFLLFAIPDLFLLSLLHPLRIYLRTQNITLPVTYSTAVSVLLHVPLNYLLVVKLEMG 236

Query: 281 VEGVALSLAWNTLNLNVGLMIYLALSSKPLKPWHGVTILSTFQGWQPLLSLAVPSAVSVC 340
           V GVA+++    LNL V L  ++  +S     W  +TI  + +GW  LLSLA+P+ VSVC
Sbjct: 237 VAGVAIAMVLTNLNLVVLLSSFVYFTSVHSDTWVPITI-DSLKGWSALLSLAIPTCVSVC 296

Query: 341 LEWWWYEIMLFLCGLLNHPQNTVAAMGILIQTTGMLYIVPFSLSAGITTRIGHALGAGEP 400
           LEWWWYE M+ LCGLL +P+ TVA+MGILIQTT ++Y+ P SLS G++TRI + LGA  P
Sbjct: 297 LEWWWYEFMIILCGLLANPRATVASMGILIQTTALVYVFPSSLSLGVSTRISNELGAKRP 356

Query: 401 IRAQWTAVIGLSTGLAFGLTAFLFMTSVRSVWGKLYTDEPEILRMISSALPILGLCEISN 460
            +A+ + +I L   +A GL A +F   VR  WG+L+T + EIL++ S ALPI+GLCE+ N
Sbjct: 357 AKARVSMIISLFCAIALGLMAMVFAVLVRHHWGRLFTTDAEILQLTSIALPIVGLCELGN 416

Query: 461 SPQTVACGVLTGTARPKLGARINLYAFYFIGLPVAVLATFTLKTGFLGLWFGLMTAQISC 520
            PQT  CGVL G ARP LGA INL +FYF+G+PVA+L  F  K GF GLWFGL+ AQ +C
Sbjct: 417 CPQTTGCGVLRGCARPTLGANINLGSFYFVGMPVAILFGFVFKQGFPGLWFGLLAAQATC 476

Query: 521 LCMLVRTLLRTDWIQQSARAVELAA 546
             +++  LLRTDW  Q+ RA EL +
Sbjct: 477 ASLMLCALLRTDWKVQAERAEELTS 500

BLAST of Lsi03G022720 vs. ExPASy Swiss-Prot
Match: O82752 (Protein DETOXIFICATION 49 OS=Arabidopsis thaliana OX=3702 GN=DTX49 PE=2 SV=1)

HSP 1 Score: 459.9 bits (1182), Expect = 4.1e-128
Identity = 244/470 (51.91%), Postives = 327/470 (69.57%), Query Frame = 0

Query: 101 EELKSLARFAGPIIMTSFLIYSRSVVSMLFLGHLGK-AELAGGSLALGFGNITGISILRG 160
           +E KS+A+ + P+I+T  L+YSRS++SMLFLG L   + L+GGSLALGF NITG S+L G
Sbjct: 31  QEAKSIAKISLPLILTGLLLYSRSMISMLFLGRLNDLSALSGGSLALGFANITGYSLLSG 90

Query: 161 LSTGMDPICCQAFGAKRWSVLSQTFLKTLCLLLLVSIPISILWLNMEPILLWLGQDPAIT 220
           LS GM+PIC QAFGAKR+ +L     +T  LLLL S+PISILWLN++ ILL+ GQD  I+
Sbjct: 91  LSIGMEPICVQAFGAKRFKLLGLALQRTTLLLLLCSLPISILWLNIKKILLFFGQDEEIS 150

Query: 221 QVAKVYMVFSIPELLAQAHHLPLRIFLRTQGITTPITVASVCSALLHPPINYFLVTYLKL 280
             A+++++FS+P+L+ Q+   P+RI+LR+Q IT P+T ++  + LLH PINY LV+ L L
Sbjct: 151 NQAEIFILFSLPDLILQSFLHPIRIYLRSQSITLPLTYSAFFAVLLHIPINYLLVSSLGL 210

Query: 281 GVEGVALSLAWNTLNLNVGLMIYLALSSKPLKPWHGVTILSTFQGWQPLLSLAVPSAVSV 340
           G++GVAL   W  +NL   L+IY+  S    K W G + +  F+GW+ L+ LA+PS VSV
Sbjct: 211 GLKGVALGAIWTNVNLLGFLIIYIVFSGVYQKTWGGFS-MDCFKGWRSLMKLAIPSCVSV 270

Query: 341 CLEWWWYEIMLFLCGLLNHPQNTVAAMGILIQTTGMLYIVPFSLSAGITTRIGHALGAGE 400
           CLEWWWYEIM+ LCGLL +PQ TVA+MGILIQTT ++YI P SLS  ++TR+G+ LGA +
Sbjct: 271 CLEWWWYEIMILLCGLLLNPQATVASMGILIQTTALIYIFPSSLSISVSTRVGNELGANQ 330

Query: 401 PIRAQWTAVIGLSTGLAFGLTAFLFMTSVRSVWGKLYTDEPEILRMISSALPILGLCEIS 460
           P +A+  A  GLS  L  GL A  F   VR+ W +L+TDE EI+++ S  LPI+GLCE+ 
Sbjct: 331 PDKARIAARTGLSLSLGLGLLAMFFALMVRNCWARLFTDEEEIVKLTSMVLPIIGLCELG 390

Query: 461 NSPQTVACGVLTGTARPKLGARINLYAFYFIGLPVAVLATFTLKTGFLGLWFGLMTAQIS 520
           N PQT  CGVL G+ARPKLGA INL  FYF+G+PVAV  +F     F GLW GL  AQ S
Sbjct: 391 NCPQTTLCGVLRGSARPKLGANINLCCFYFVGMPVAVWLSFFSGFDFKGLWLGLFAAQGS 450

Query: 521 CLCMLVRTLLRTDWIQQSARAVELAATVGEEIAKEEEDVESGLIDDNADL 570
           CL  ++  L RTDW  +  RA EL   +      +E+D  +  + D+ D+
Sbjct: 451 CLISMLVVLARTDWEVEVHRAKEL---MTRSCDGDEDDGNTPFLLDSLDI 496

BLAST of Lsi03G022720 vs. ExPASy Swiss-Prot
Match: Q9SZE2 (Protein DETOXIFICATION 51 OS=Arabidopsis thaliana OX=3702 GN=DTX51 PE=2 SV=1)

HSP 1 Score: 424.5 bits (1090), Expect = 1.9e-117
Identity = 230/469 (49.04%), Postives = 310/469 (66.10%), Query Frame = 0

Query: 102 ELKSLARFAGPIIMTSFLIYSRSVVSMLFLGHLGKAELAGGSLALGFGNITGISILRGLS 161
           E KSL   A PI +T+ ++Y RS VSM FLG LG  ELA GSLA+ F NITG S+L GL+
Sbjct: 56  EAKSLFTLAFPIAVTALVLYLRSAVSMFFLGQLGDLELAAGSLAIAFANITGYSVLSGLA 115

Query: 162 TGMDPICCQAFGAKRWSVLSQTFLKTLCLLLLVSIPISILWLNMEPILLWLGQDPAITQV 221
            GM+P+C QAFGA R+ +LS T  +T+  LL+  +PIS+LW N+  I ++L QDP I ++
Sbjct: 116 LGMEPLCSQAFGAHRFKLLSLTLHRTVVFLLVCCVPISVLWFNVGKISVYLHQDPDIAKL 175

Query: 222 AKVYMVFSIPELLAQAHHLPLRIFLRTQGITTPITVASVCSALLHPPINYFLVTYLKLGV 281
           A+ Y++FS+P+LL      P+RI+LR QGI  P+T+AS+  A+ H P N FLV+YL+LG+
Sbjct: 176 AQTYLIFSLPDLLTNTLLHPIRIYLRAQGIIHPVTLASLSGAVFHLPANLFLVSYLRLGL 235

Query: 282 EGVALSLAWNTLNLNVGLMIYLALSSKPLKPWHGVTILSTFQGWQPLLSLAVPSAVSVCL 341
            GVA++ +   + +   L+ Y+  S      W   T    F+GW PLL LA PS VSVCL
Sbjct: 236 TGVAVASSITNIFVVAFLVCYVWASGLHAPTWTDPT-RDCFRGWAPLLRLAGPSCVSVCL 295

Query: 342 EWWWYEIMLFLCGLLNHPQNTVAAMGILIQTTGMLYIVPFSLSAGITTRIGHALGAGEPI 401
           EWWWYEIM+ LCGLL +P++TVAAMG+LIQTT  LY+ P SLS  ++TR+G+ LGA  P 
Sbjct: 296 EWWWYEIMIVLCGLLVNPRSTVAAMGVLIQTTSFLYVFPSSLSFAVSTRVGNELGANRPK 355

Query: 402 RAQWTAVIGLSTGLAFGLTAFLFMTSVRSVWGKLYTDEPEILRMISSALPILGLCEISNS 461
            A+ TA + +      G+ A  F  SVR+ WG+++T + EIL++ ++ALPILGLCEI N 
Sbjct: 356 TAKLTATVAIVFAAVTGIIAAAFAYSVRNAWGRIFTGDKEILQLTAAALPILGLCEIGNC 415

Query: 462 PQTVACGVLTGTARPKLGARINLYAFYFIGLPVAVLATFTLKTGFLGLWFGLMTAQISCL 521
           PQTV CGV+ GTARP   A +NL AFY +G+PVAV   F    GF GLW GL+ AQISC 
Sbjct: 416 PQTVGCGVVRGTARPSTAANVNLGAFYLVGMPVAVGLGFWAGIGFNGLWVGLLAAQISCA 475

Query: 522 CMLVRTLLRTDWIQQSARAVEL--AATVGEEIAKEEEDVESGLIDDNAD 569
            +++  +  TDW  ++ +A  L  A TV  +I K    V +  ID   D
Sbjct: 476 GLMMYVVGTTDWESEAKKAQTLTCAETVENDIIKA---VVASTIDGECD 520

BLAST of Lsi03G022720 vs. ExPASy Swiss-Prot
Match: Q4PSF4 (Protein DETOXIFICATION 52 OS=Arabidopsis thaliana OX=3702 GN=DTX52 PE=2 SV=1)

HSP 1 Score: 424.1 bits (1089), Expect = 2.5e-117
Identity = 215/454 (47.36%), Postives = 310/454 (68.28%), Query Frame = 0

Query: 98  QMKEELKSLARFAGPIIMTSFLIYSRSVVSMLFLGHLGKAELAGGSLALGFGNITGISIL 157
           ++K E +SL   A P I+ + ++Y+RS +SMLFLGH+G+ ELAGGSLA+ F NITG S+L
Sbjct: 32  ELKSEARSLFSLAFPTILAALILYARSAISMLFLGHIGELELAGGSLAIAFANITGYSVL 91

Query: 158 RGLSTGMDPICCQAFGAKRWSVLSQTFLKTLCLLLLVSIPISILWLNMEPILLWLGQDPA 217
            GL+ GMDP+C QAFGA R  +LS T  +T+  LL  S+ I  LWLN+  I+++L QDP+
Sbjct: 92  AGLALGMDPLCSQAFGAGRPKLLSLTLQRTVLFLLTSSVVIVALWLNLGKIMIYLHQDPS 151

Query: 218 ITQVAKVYMVFSIPELLAQAHHLPLRIFLRTQGITTPITVASVCSALLHPPINYFLVTYL 277
           I+ +A+ Y++ SIP+LL  +   PLRI+LR QGIT+P+T+A++   + H P+N+FLV+YL
Sbjct: 152 ISSLAQTYILCSIPDLLTNSFLHPLRIYLRAQGITSPLTLATLAGTIFHIPMNFFLVSYL 211

Query: 278 KLGVEGVALSLAWNTLNLNVGLMIYLALSSKPLKPWHGVTILSTFQGWQPLLSLAVPSAV 337
             G  GV+++ A + L + + L+ ++ ++      W   +    F+ W P+++LA+PS +
Sbjct: 212 GWGFMGVSMAAAASNLLVVIFLVAHVWIAGLHQPTWTRPS-SECFKDWGPVVTLAIPSCI 271

Query: 338 SVCLEWWWYEIMLFLCGLLNHPQNTVAAMGILIQTTGMLYIVPFSLSAGITTRIGHALGA 397
            VCLEWWWYEIM  LCGLL  P   VA+MGILIQTT +LYI P SL   ++TR+G+ LG+
Sbjct: 272 GVCLEWWWYEIMTVLCGLLIDPSTPVASMGILIQTTSLLYIFPSSLGLAVSTRVGNELGS 331

Query: 398 GEPIRAQWTAVIGLSTGLAFGLTAFLFMTSVRSVWGKLYTDEPEILRMISSALPILGLCE 457
             P +A+ +A++ +S     GLTA  F   V  VWG ++T++  I+++ ++ALPILGLCE
Sbjct: 332 NRPNKARLSAIVAVSFAGVMGLTASAFAWGVSDVWGWIFTNDVAIIKLTAAALPILGLCE 391

Query: 458 ISNSPQTVACGVLTGTARPKLGARINLYAFYFIGLPVAVLATFTLKTGFLGLWFGLMTAQ 517
           + N PQTV CGV+ GTARP + A INL AFY +G PVAV  TF    GF GLW GL+ AQ
Sbjct: 392 LGNCPQTVGCGVVRGTARPSMAANINLGAFYLVGTPVAVGLTFWAAYGFCGLWVGLLAAQ 451

Query: 518 ISCLCMLVRTLLRTDWIQQSARAVELAATVGEEI 552
           I C  M++  +  TDW +++ RA +L  T G ++
Sbjct: 452 ICCAAMMLYVVATTDWEKEAIRARKLTCTEGVDV 484

BLAST of Lsi03G022720 vs. ExPASy TrEMBL
Match: A0A0A0LCM3 (Protein DETOXIFICATION OS=Cucumis sativus OX=3659 GN=Csa_3G728650 PE=3 SV=1)

HSP 1 Score: 920.6 bits (2378), Expect = 3.2e-264
Identity = 478/521 (91.75%), Postives = 492/521 (94.43%), Query Frame = 0

Query: 64  TTTKDELKMSTTDSQ---------------GFFRRLLPLNALPQCLPPQQMKEELKSLAR 123
           TTTK ELK+ +++S                GFFRRLLPL+ALP  LPPQQMKEELKSLAR
Sbjct: 10  TTTKHELKIMSSNSNSNSSSNNSNLSGTIPGFFRRLLPLHALPHGLPPQQMKEELKSLAR 69

Query: 124 FAGPIIMTSFLIYSRSVVSMLFLGHLGKAELAGGSLALGFGNITGISILRGLSTGMDPIC 183
           FAGPIIMTSFL+YSRSVVSMLFLGHLGKAELAGGSLALGFGNITGISILRGLSTGMDPIC
Sbjct: 70  FAGPIIMTSFLMYSRSVVSMLFLGHLGKAELAGGSLALGFGNITGISILRGLSTGMDPIC 129

Query: 184 CQAFGAKRWSVLSQTFLKTLCLLLLVSIPISILWLNMEPILLWLGQDPAITQVAKVYMVF 243
           CQAFGAKRWSVLSQTFLKTLCLLLLVSIPISILWLNMEPILLWLGQDPAITQVAKVYMVF
Sbjct: 130 CQAFGAKRWSVLSQTFLKTLCLLLLVSIPISILWLNMEPILLWLGQDPAITQVAKVYMVF 189

Query: 244 SIPELLAQAHHLPLRIFLRTQGITTPITVASVCSALLHPPINYFLVTYLKLGVEGVALSL 303
           SIPELLAQAHHLPLRIFLRTQGITTPITVASV SALLHP INYFLVTYLKLGVEGVALSL
Sbjct: 190 SIPELLAQAHHLPLRIFLRTQGITTPITVASVASALLHPLINYFLVTYLKLGVEGVALSL 249

Query: 304 AWNTLNLNVGLMIYLALSSKPLKPWHGVTILSTFQGWQPLLSLAVPSAVSVCLEWWWYEI 363
           AWNTLNLNVGLMIYLALSSKPLKPWHGVTILSTFQGW+PLLSLA+PSAVSVCLEWWWYEI
Sbjct: 250 AWNTLNLNVGLMIYLALSSKPLKPWHGVTILSTFQGWEPLLSLAIPSAVSVCLEWWWYEI 309

Query: 364 MLFLCGLLNHPQNTVAAMGILIQTTGMLYIVPFSLSAGITTRIGHALGAGEPIRAQWTAV 423
           MLFLCGLLN+PQNTV+AMGILIQTTGMLYIVPFSLSAGITTRIGHALGAGEPIRAQWTA+
Sbjct: 310 MLFLCGLLNNPQNTVSAMGILIQTTGMLYIVPFSLSAGITTRIGHALGAGEPIRAQWTAI 369

Query: 424 IGLSTGLAFGLTAFLFMTSVRSVWGKLYTDEPEILRMISSALPILGLCEISNSPQTVACG 483
           IGLSTG AFG+TAF FMTSVRSVWGKLYTDEPEILRMISSALP+LGLCEISNSPQTVACG
Sbjct: 370 IGLSTGFAFGVTAFFFMTSVRSVWGKLYTDEPEILRMISSALPVLGLCEISNSPQTVACG 429

Query: 484 VLTGTARPKLGARINLYAFYFIGLPVAVLATFTLKTGFLGLWFGLMTAQISCLCMLVRTL 543
           VLTGTARPKLGARINLYAFYFIGLPVAVLATFTLKTGFLGLWFGLM AQISCLCMLVRTL
Sbjct: 430 VLTGTARPKLGARINLYAFYFIGLPVAVLATFTLKTGFLGLWFGLMIAQISCLCMLVRTL 489

Query: 544 LRTDWIQQSARAVELAATVGEEIAKEEEDVESG-LIDDNAD 569
           LRTDWIQQS RAVELAA VGEE AKEEEDVE+G LIDDNAD
Sbjct: 490 LRTDWIQQSVRAVELAAAVGEETAKEEEDVETGALIDDNAD 530

BLAST of Lsi03G022720 vs. ExPASy TrEMBL
Match: A0A6J1DW06 (Protein DETOXIFICATION OS=Momordica charantia OX=3673 GN=LOC111024019 PE=3 SV=1)

HSP 1 Score: 914.1 bits (2361), Expect = 3.0e-262
Identity = 465/503 (92.45%), Postives = 485/503 (96.42%), Query Frame = 0

Query: 72  MSTTDSQ-----GFFRRLLPLNALPQCLPPQQMKEELKSLARFAGPIIMTSFLIYSRSVV 131
           MS T S+     GF RRLLP++ LPQ LPP +MKEELKSLA+FAGPIIMTSFLIYSRSVV
Sbjct: 1   MSDTRSEGGFIDGFLRRLLPVDGLPQGLPPDEMKEELKSLAKFAGPIIMTSFLIYSRSVV 60

Query: 132 SMLFLGHLGKAELAGGSLALGFGNITGISILRGLSTGMDPICCQAFGAKRWSVLSQTFLK 191
           SMLFLGHLGKAELAGGSLALGFGNITGISILRGLSTGMDPICCQAFGAKRWS+LSQTFLK
Sbjct: 61  SMLFLGHLGKAELAGGSLALGFGNITGISILRGLSTGMDPICCQAFGAKRWSLLSQTFLK 120

Query: 192 TLCLLLLVSIPISILWLNMEPILLWLGQDPAITQVAKVYMVFSIPELLAQAHHLPLRIFL 251
           TLCLLLLVS+PISILWLNMEPILLWLGQDPAITQVAKVYMVFSIPELLAQAHHLPLRIFL
Sbjct: 121 TLCLLLLVSVPISILWLNMEPILLWLGQDPAITQVAKVYMVFSIPELLAQAHHLPLRIFL 180

Query: 252 RTQGITTPITVASVCSALLHPPINYFLVTYLKLGVEGVALSLAWNTLNLNVGLMIYLALS 311
           RTQGITTPITVAS+CSA+LHPPINYFLVTY+KLGVEGVALSLAWNTLNLNVGLMIYLALS
Sbjct: 181 RTQGITTPITVASICSAVLHPPINYFLVTYMKLGVEGVALSLAWNTLNLNVGLMIYLALS 240

Query: 312 SKPLKPWHGVTILSTFQGWQPLLSLAVPSAVSVCLEWWWYEIMLFLCGLLNHPQNTVAAM 371
           SKPLKPWHGVTILSTFQGWQPLLSLAVPSAVSVCLEWWWYEIMLFLCGLL++PQNTVAAM
Sbjct: 241 SKPLKPWHGVTILSTFQGWQPLLSLAVPSAVSVCLEWWWYEIMLFLCGLLSNPQNTVAAM 300

Query: 372 GILIQTTGMLYIVPFSLSAGITTRIGHALGAGEPIRAQWTAVIGLSTGLAFGLTAFLFMT 431
           GILIQTTGMLYIVPFSLSAGITTRIGHALGAG+P+RAQWTAVIGLS+GLAFGL AF+FMT
Sbjct: 301 GILIQTTGMLYIVPFSLSAGITTRIGHALGAGQPVRAQWTAVIGLSSGLAFGLAAFVFMT 360

Query: 432 SVRSVWGKLYTDEPEILRMISSALPILGLCEISNSPQTVACGVLTGTARPKLGARINLYA 491
           SVRSVWGKLYTDEP+IL+MISSALPILGLCEISNSPQTVACGVLTGTARPKLGARINLYA
Sbjct: 361 SVRSVWGKLYTDEPQILQMISSALPILGLCEISNSPQTVACGVLTGTARPKLGARINLYA 420

Query: 492 FYFIGLPVAVLATFTLKTGFLGLWFGLMTAQISCLCMLVRTLLRTDWIQQSARAVELAAT 551
           FYFIGLPVAVLATFT  TGFLGLWFGLMTAQISCLCMLVRTLLRTDW+QQS RAVELA T
Sbjct: 421 FYFIGLPVAVLATFTFNTGFLGLWFGLMTAQISCLCMLVRTLLRTDWVQQSTRAVELATT 480

Query: 552 VGEEIAKEEEDVESGLIDDNADL 570
            GEE AKE+EDVESGLIDDNADL
Sbjct: 481 AGEETAKEDEDVESGLIDDNADL 503

BLAST of Lsi03G022720 vs. ExPASy TrEMBL
Match: A0A1S4DUK8 (Protein DETOXIFICATION OS=Cucumis melo OX=3656 GN=LOC103486234 PE=3 SV=1)

HSP 1 Score: 890.6 bits (2300), Expect = 3.5e-255
Identity = 460/472 (97.46%), Postives = 464/472 (98.31%), Query Frame = 0

Query: 98  QMKEELKSLARFAGPIIMTSFLIYSRSVVSMLFLGHLGKAELAGGSLALGFGNITGISIL 157
           QMKEELKSLARFAGPIIMTSFLIYSRSVVSMLFLGHLGKAELAGGSLALGFGNITGISIL
Sbjct: 2   QMKEELKSLARFAGPIIMTSFLIYSRSVVSMLFLGHLGKAELAGGSLALGFGNITGISIL 61

Query: 158 RGLSTGMDPICCQAFGAKRWSVLSQTFLKTLCLLLLVSIPISILWLNMEPILLWLGQDPA 217
           RGLSTGMDPICCQAFGAKRWSVLSQTFLKTLCLLLLVSIPISILWLNMEPILLWLGQDPA
Sbjct: 62  RGLSTGMDPICCQAFGAKRWSVLSQTFLKTLCLLLLVSIPISILWLNMEPILLWLGQDPA 121

Query: 218 ITQVAKVYMVFSIPELLAQAHHLPLRIFLRTQGITTPITVASVCSALLHPPINYFLVTYL 277
           ITQVAKVYMVFSIPELLAQAHHLPLRIFLRTQGITTPITVASV SALLHP INYFLVTYL
Sbjct: 122 ITQVAKVYMVFSIPELLAQAHHLPLRIFLRTQGITTPITVASVASALLHPLINYFLVTYL 181

Query: 278 KLGVEGVALSLAWNTLNLNVGLMIYLALSSKPLKPWHGVTILSTFQGWQPLLSLAVPSAV 337
           KLGVEGVALSLAWNTLNLNVGLMIYLALSSKPLKPWHGVTI STFQGWQPLLSLA+PSAV
Sbjct: 182 KLGVEGVALSLAWNTLNLNVGLMIYLALSSKPLKPWHGVTIPSTFQGWQPLLSLAIPSAV 241

Query: 338 SVCLEWWWYEIMLFLCGLLNHPQNTVAAMGILIQTTGMLYIVPFSLSAGITTRIGHALGA 397
           SVCLEWWWYEIMLFLCGLLN+PQNTVAAMGILIQTTGMLYIVPFSLSAGITTRIGHALGA
Sbjct: 242 SVCLEWWWYEIMLFLCGLLNNPQNTVAAMGILIQTTGMLYIVPFSLSAGITTRIGHALGA 301

Query: 398 GEPIRAQWTAVIGLSTGLAFGLTAFLFMTSVRSVWGKLYTDEPEILRMISSALPILGLCE 457
           GEPIRAQWTA+IGLSTG AFGLTAFLFMTSVRSVWGKLYTDEPEILRMISSALPILGLCE
Sbjct: 302 GEPIRAQWTAIIGLSTGFAFGLTAFLFMTSVRSVWGKLYTDEPEILRMISSALPILGLCE 361

Query: 458 ISNSPQTVACGVLTGTARPKLGARINLYAFYFIGLPVAVLATFTLKTGFLGLWFGLMTAQ 517
           ISNSPQTVACGVLTGTARPKLGARINLYAFYFIGLPVAVLATFTLKTGFLGLWFGLMTAQ
Sbjct: 362 ISNSPQTVACGVLTGTARPKLGARINLYAFYFIGLPVAVLATFTLKTGFLGLWFGLMTAQ 421

Query: 518 ISCLCMLVRTLLRTDWIQQSARAVELAATVGEEIAKEEEDVESG-LIDDNAD 569
           ISCLCMLVRTLLRTDWIQQS RAVELAA VGEE AKEEEDVE+G LIDDNAD
Sbjct: 422 ISCLCMLVRTLLRTDWIQQSVRAVELAAAVGEETAKEEEDVETGALIDDNAD 473

BLAST of Lsi03G022720 vs. ExPASy TrEMBL
Match: A0A6J1IZQ7 (Protein DETOXIFICATION OS=Cucurbita maxima OX=3661 GN=LOC111481417 PE=3 SV=1)

HSP 1 Score: 890.2 bits (2299), Expect = 4.6e-255
Identity = 449/487 (92.20%), Postives = 469/487 (96.30%), Query Frame = 0

Query: 80  FFRRLLPLNALPQCLPPQQMKEELKSLARFAGPIIMTSFLIYSRSVVSMLFLGHLGKAEL 139
           FFRRL PLNALPQ LPP QMKEELKSLARF+GPIIMTSFL+YSRSVVSMLFLGHLGKAEL
Sbjct: 43  FFRRLFPLNALPQGLPPHQMKEELKSLARFSGPIIMTSFLLYSRSVVSMLFLGHLGKAEL 102

Query: 140 AGGSLALGFGNITGISILRGLSTGMDPICCQAFGAKRWSVLSQTFLKTLCLLLLVSIPIS 199
           AGGSLALGFGNITGISILRGLSTGMDPICCQAFGAKRWSVLSQTFLKTLCLLLLVS+PIS
Sbjct: 103 AGGSLALGFGNITGISILRGLSTGMDPICCQAFGAKRWSVLSQTFLKTLCLLLLVSLPIS 162

Query: 200 ILWLNMEPILLWLGQDPAITQVAKVYMVFSIPELLAQAHHLPLRIFLRTQGITTPITVAS 259
           +LWLNMEPILLWLGQDPAITQVAKVYM+FSIPELLAQAHHLPLRIFLRTQGITTPITVAS
Sbjct: 163 VLWLNMEPILLWLGQDPAITQVAKVYMLFSIPELLAQAHHLPLRIFLRTQGITTPITVAS 222

Query: 260 VCSALLHPPINYFLVTYLKLGVEGVALSLAWNTLNLNVGLMIYLALSSKPLKPWHGVTIL 319
           +CSALLHPPINYFLV+YL LGVEGVALSLAWNT NLN+GLM+YL LSSKPLKPWHGVTIL
Sbjct: 223 ICSALLHPPINYFLVSYLNLGVEGVALSLAWNTFNLNLGLMLYLVLSSKPLKPWHGVTIL 282

Query: 320 STFQGWQPLLSLAVPSAVSVCLEWWWYEIMLFLCGLLNHPQNTVAAMGILIQTTGMLYIV 379
           STFQGWQPLLSLAVPSAVSVCLEWWWYEIMLFLCGLL++PQNTVAAMGILIQTTGMLYIV
Sbjct: 283 STFQGWQPLLSLAVPSAVSVCLEWWWYEIMLFLCGLLSNPQNTVAAMGILIQTTGMLYIV 342

Query: 380 PFSLSAGITTRIGHALGAGEPIRAQWTAVIGLSTGLAFGLTAFLFMTSVRSVWGKLYTDE 439
           PFSLSAGITTR+G ALGAGEPIRAQWTAVIGLSTGLAFG+TAF FMTSVRSVWGKLYTDE
Sbjct: 343 PFSLSAGITTRVGQALGAGEPIRAQWTAVIGLSTGLAFGVTAFFFMTSVRSVWGKLYTDE 402

Query: 440 PEILRMISSALPILGLCEISNSPQTVACGVLTGTARPKLGARINLYAFYFIGLPVAVLAT 499
           P+IL MISSALP+LG+CEISNSPQTVACGVLTGTARPKLGARINLYAFYFIGLPVAV+AT
Sbjct: 403 PQILHMISSALPVLGVCEISNSPQTVACGVLTGTARPKLGARINLYAFYFIGLPVAVVAT 462

Query: 500 FTLKTGFLGLWFGLMTAQISCLCMLVRTLLRTDWIQQSARAVELAATVGEEIAKEEEDVE 559
           FTLK GFLGLW GLMTAQISCLCMLV TLLRTDWIQQSARAVE+AA VGEE  KEEE VE
Sbjct: 463 FTLKAGFLGLWIGLMTAQISCLCMLVHTLLRTDWIQQSARAVEMAAIVGEESGKEEEGVE 522

Query: 560 SGLIDDN 567
            GL++ N
Sbjct: 523 CGLMEGN 529

BLAST of Lsi03G022720 vs. ExPASy TrEMBL
Match: A0A1S3B4Z3 (Protein DETOXIFICATION OS=Cucumis melo OX=3656 GN=LOC103486234 PE=3 SV=1)

HSP 1 Score: 888.6 bits (2295), Expect = 1.3e-254
Identity = 459/471 (97.45%), Postives = 463/471 (98.30%), Query Frame = 0

Query: 99  MKEELKSLARFAGPIIMTSFLIYSRSVVSMLFLGHLGKAELAGGSLALGFGNITGISILR 158
           MKEELKSLARFAGPIIMTSFLIYSRSVVSMLFLGHLGKAELAGGSLALGFGNITGISILR
Sbjct: 1   MKEELKSLARFAGPIIMTSFLIYSRSVVSMLFLGHLGKAELAGGSLALGFGNITGISILR 60

Query: 159 GLSTGMDPICCQAFGAKRWSVLSQTFLKTLCLLLLVSIPISILWLNMEPILLWLGQDPAI 218
           GLSTGMDPICCQAFGAKRWSVLSQTFLKTLCLLLLVSIPISILWLNMEPILLWLGQDPAI
Sbjct: 61  GLSTGMDPICCQAFGAKRWSVLSQTFLKTLCLLLLVSIPISILWLNMEPILLWLGQDPAI 120

Query: 219 TQVAKVYMVFSIPELLAQAHHLPLRIFLRTQGITTPITVASVCSALLHPPINYFLVTYLK 278
           TQVAKVYMVFSIPELLAQAHHLPLRIFLRTQGITTPITVASV SALLHP INYFLVTYLK
Sbjct: 121 TQVAKVYMVFSIPELLAQAHHLPLRIFLRTQGITTPITVASVASALLHPLINYFLVTYLK 180

Query: 279 LGVEGVALSLAWNTLNLNVGLMIYLALSSKPLKPWHGVTILSTFQGWQPLLSLAVPSAVS 338
           LGVEGVALSLAWNTLNLNVGLMIYLALSSKPLKPWHGVTI STFQGWQPLLSLA+PSAVS
Sbjct: 181 LGVEGVALSLAWNTLNLNVGLMIYLALSSKPLKPWHGVTIPSTFQGWQPLLSLAIPSAVS 240

Query: 339 VCLEWWWYEIMLFLCGLLNHPQNTVAAMGILIQTTGMLYIVPFSLSAGITTRIGHALGAG 398
           VCLEWWWYEIMLFLCGLLN+PQNTVAAMGILIQTTGMLYIVPFSLSAGITTRIGHALGAG
Sbjct: 241 VCLEWWWYEIMLFLCGLLNNPQNTVAAMGILIQTTGMLYIVPFSLSAGITTRIGHALGAG 300

Query: 399 EPIRAQWTAVIGLSTGLAFGLTAFLFMTSVRSVWGKLYTDEPEILRMISSALPILGLCEI 458
           EPIRAQWTA+IGLSTG AFGLTAFLFMTSVRSVWGKLYTDEPEILRMISSALPILGLCEI
Sbjct: 301 EPIRAQWTAIIGLSTGFAFGLTAFLFMTSVRSVWGKLYTDEPEILRMISSALPILGLCEI 360

Query: 459 SNSPQTVACGVLTGTARPKLGARINLYAFYFIGLPVAVLATFTLKTGFLGLWFGLMTAQI 518
           SNSPQTVACGVLTGTARPKLGARINLYAFYFIGLPVAVLATFTLKTGFLGLWFGLMTAQI
Sbjct: 361 SNSPQTVACGVLTGTARPKLGARINLYAFYFIGLPVAVLATFTLKTGFLGLWFGLMTAQI 420

Query: 519 SCLCMLVRTLLRTDWIQQSARAVELAATVGEEIAKEEEDVESG-LIDDNAD 569
           SCLCMLVRTLLRTDWIQQS RAVELAA VGEE AKEEEDVE+G LIDDNAD
Sbjct: 421 SCLCMLVRTLLRTDWIQQSVRAVELAAAVGEETAKEEEDVETGALIDDNAD 471

BLAST of Lsi03G022720 vs. NCBI nr
Match: XP_038904374.1 (protein DETOXIFICATION 53 [Benincasa hispida])

HSP 1 Score: 952.6 bits (2461), Expect = 1.5e-273
Identity = 491/519 (94.61%), Postives = 500/519 (96.34%), Query Frame = 0

Query: 61  MAKTTTKDELKMSTTDSQ----------GFFRRLLPLNALPQCLPPQQMKEELKSLARFA 120
           MAKT  KDELKMS+T S           GFFRRLLPL+ALP  LPPQQMKEELKSLARFA
Sbjct: 1   MAKTIAKDELKMSSTSSSNSSNSNTTLGGFFRRLLPLHALPNGLPPQQMKEELKSLARFA 60

Query: 121 GPIIMTSFLIYSRSVVSMLFLGHLGKAELAGGSLALGFGNITGISILRGLSTGMDPICCQ 180
           GPIIMTSFLIYSRSVVSMLFLGHLGKAELAGGSLALGFGNITGISIL+GLSTGMDPICCQ
Sbjct: 61  GPIIMTSFLIYSRSVVSMLFLGHLGKAELAGGSLALGFGNITGISILKGLSTGMDPICCQ 120

Query: 181 AFGAKRWSVLSQTFLKTLCLLLLVSIPISILWLNMEPILLWLGQDPAITQVAKVYMVFSI 240
           AFGAKRWSVLSQTFLKTLCLLLLVS+PISILWLNMEPILLWLGQDPAI QVAKVYMVFSI
Sbjct: 121 AFGAKRWSVLSQTFLKTLCLLLLVSVPISILWLNMEPILLWLGQDPAIAQVAKVYMVFSI 180

Query: 241 PELLAQAHHLPLRIFLRTQGITTPITVASVCSALLHPPINYFLVTYLKLGVEGVALSLAW 300
           PELLAQAHHLPLRIFLRTQGITTPITVASVCSALLHPPINYFLVTYLKLGVEGVALSLAW
Sbjct: 181 PELLAQAHHLPLRIFLRTQGITTPITVASVCSALLHPPINYFLVTYLKLGVEGVALSLAW 240

Query: 301 NTLNLNVGLMIYLALSSKPLKPWHGVTILSTFQGWQPLLSLAVPSAVSVCLEWWWYEIML 360
           NTLNLNVGLMIYLALSSKPLKPWHGVTILSTFQGWQPLLSLAVPSA+SVCLEWWWYEIML
Sbjct: 241 NTLNLNVGLMIYLALSSKPLKPWHGVTILSTFQGWQPLLSLAVPSAISVCLEWWWYEIML 300

Query: 361 FLCGLLNHPQNTVAAMGILIQTTGMLYIVPFSLSAGITTRIGHALGAGEPIRAQWTAVIG 420
           FLCGLLN+PQNTVAAMGILIQTTGMLYIVPFSLSAGITTRIGHALGAGEPIRAQWTA+IG
Sbjct: 301 FLCGLLNNPQNTVAAMGILIQTTGMLYIVPFSLSAGITTRIGHALGAGEPIRAQWTAIIG 360

Query: 421 LSTGLAFGLTAFLFMTSVRSVWGKLYTDEPEILRMISSALPILGLCEISNSPQTVACGVL 480
           LSTGLAFGLTAFLFMTSVRSVWGKLYTDEPEIL MISSALPILGLCEISNSPQTVACGVL
Sbjct: 361 LSTGLAFGLTAFLFMTSVRSVWGKLYTDEPEILHMISSALPILGLCEISNSPQTVACGVL 420

Query: 481 TGTARPKLGARINLYAFYFIGLPVAVLATFTLKTGFLGLWFGLMTAQISCLCMLVRTLLR 540
           TGTARPKLGARINLYAFYFIGLPVAVL+TFTLKTGFLGLWFGLMTAQISCLCMLVRTLLR
Sbjct: 421 TGTARPKLGARINLYAFYFIGLPVAVLSTFTLKTGFLGLWFGLMTAQISCLCMLVRTLLR 480

Query: 541 TDWIQQSARAVELAATVGEEIAKEEEDVESGLIDDNADL 570
           TDWIQQSARAVELAATVGEE AKEEEDVESGL+DDNADL
Sbjct: 481 TDWIQQSARAVELAATVGEETAKEEEDVESGLLDDNADL 519

BLAST of Lsi03G022720 vs. NCBI nr
Match: XP_004137889.2 (protein DETOXIFICATION 53 isoform X1 [Cucumis sativus] >KGN58699.1 hypothetical protein Csa_001442 [Cucumis sativus])

HSP 1 Score: 920.6 bits (2378), Expect = 6.5e-264
Identity = 478/521 (91.75%), Postives = 492/521 (94.43%), Query Frame = 0

Query: 64  TTTKDELKMSTTDSQ---------------GFFRRLLPLNALPQCLPPQQMKEELKSLAR 123
           TTTK ELK+ +++S                GFFRRLLPL+ALP  LPPQQMKEELKSLAR
Sbjct: 10  TTTKHELKIMSSNSNSNSSSNNSNLSGTIPGFFRRLLPLHALPHGLPPQQMKEELKSLAR 69

Query: 124 FAGPIIMTSFLIYSRSVVSMLFLGHLGKAELAGGSLALGFGNITGISILRGLSTGMDPIC 183
           FAGPIIMTSFL+YSRSVVSMLFLGHLGKAELAGGSLALGFGNITGISILRGLSTGMDPIC
Sbjct: 70  FAGPIIMTSFLMYSRSVVSMLFLGHLGKAELAGGSLALGFGNITGISILRGLSTGMDPIC 129

Query: 184 CQAFGAKRWSVLSQTFLKTLCLLLLVSIPISILWLNMEPILLWLGQDPAITQVAKVYMVF 243
           CQAFGAKRWSVLSQTFLKTLCLLLLVSIPISILWLNMEPILLWLGQDPAITQVAKVYMVF
Sbjct: 130 CQAFGAKRWSVLSQTFLKTLCLLLLVSIPISILWLNMEPILLWLGQDPAITQVAKVYMVF 189

Query: 244 SIPELLAQAHHLPLRIFLRTQGITTPITVASVCSALLHPPINYFLVTYLKLGVEGVALSL 303
           SIPELLAQAHHLPLRIFLRTQGITTPITVASV SALLHP INYFLVTYLKLGVEGVALSL
Sbjct: 190 SIPELLAQAHHLPLRIFLRTQGITTPITVASVASALLHPLINYFLVTYLKLGVEGVALSL 249

Query: 304 AWNTLNLNVGLMIYLALSSKPLKPWHGVTILSTFQGWQPLLSLAVPSAVSVCLEWWWYEI 363
           AWNTLNLNVGLMIYLALSSKPLKPWHGVTILSTFQGW+PLLSLA+PSAVSVCLEWWWYEI
Sbjct: 250 AWNTLNLNVGLMIYLALSSKPLKPWHGVTILSTFQGWEPLLSLAIPSAVSVCLEWWWYEI 309

Query: 364 MLFLCGLLNHPQNTVAAMGILIQTTGMLYIVPFSLSAGITTRIGHALGAGEPIRAQWTAV 423
           MLFLCGLLN+PQNTV+AMGILIQTTGMLYIVPFSLSAGITTRIGHALGAGEPIRAQWTA+
Sbjct: 310 MLFLCGLLNNPQNTVSAMGILIQTTGMLYIVPFSLSAGITTRIGHALGAGEPIRAQWTAI 369

Query: 424 IGLSTGLAFGLTAFLFMTSVRSVWGKLYTDEPEILRMISSALPILGLCEISNSPQTVACG 483
           IGLSTG AFG+TAF FMTSVRSVWGKLYTDEPEILRMISSALP+LGLCEISNSPQTVACG
Sbjct: 370 IGLSTGFAFGVTAFFFMTSVRSVWGKLYTDEPEILRMISSALPVLGLCEISNSPQTVACG 429

Query: 484 VLTGTARPKLGARINLYAFYFIGLPVAVLATFTLKTGFLGLWFGLMTAQISCLCMLVRTL 543
           VLTGTARPKLGARINLYAFYFIGLPVAVLATFTLKTGFLGLWFGLM AQISCLCMLVRTL
Sbjct: 430 VLTGTARPKLGARINLYAFYFIGLPVAVLATFTLKTGFLGLWFGLMIAQISCLCMLVRTL 489

Query: 544 LRTDWIQQSARAVELAATVGEEIAKEEEDVESG-LIDDNAD 569
           LRTDWIQQS RAVELAA VGEE AKEEEDVE+G LIDDNAD
Sbjct: 490 LRTDWIQQSVRAVELAAAVGEETAKEEEDVETGALIDDNAD 530

BLAST of Lsi03G022720 vs. NCBI nr
Match: XP_022157269.1 (protein DETOXIFICATION 53 [Momordica charantia])

HSP 1 Score: 914.1 bits (2361), Expect = 6.1e-262
Identity = 465/503 (92.45%), Postives = 485/503 (96.42%), Query Frame = 0

Query: 72  MSTTDSQ-----GFFRRLLPLNALPQCLPPQQMKEELKSLARFAGPIIMTSFLIYSRSVV 131
           MS T S+     GF RRLLP++ LPQ LPP +MKEELKSLA+FAGPIIMTSFLIYSRSVV
Sbjct: 1   MSDTRSEGGFIDGFLRRLLPVDGLPQGLPPDEMKEELKSLAKFAGPIIMTSFLIYSRSVV 60

Query: 132 SMLFLGHLGKAELAGGSLALGFGNITGISILRGLSTGMDPICCQAFGAKRWSVLSQTFLK 191
           SMLFLGHLGKAELAGGSLALGFGNITGISILRGLSTGMDPICCQAFGAKRWS+LSQTFLK
Sbjct: 61  SMLFLGHLGKAELAGGSLALGFGNITGISILRGLSTGMDPICCQAFGAKRWSLLSQTFLK 120

Query: 192 TLCLLLLVSIPISILWLNMEPILLWLGQDPAITQVAKVYMVFSIPELLAQAHHLPLRIFL 251
           TLCLLLLVS+PISILWLNMEPILLWLGQDPAITQVAKVYMVFSIPELLAQAHHLPLRIFL
Sbjct: 121 TLCLLLLVSVPISILWLNMEPILLWLGQDPAITQVAKVYMVFSIPELLAQAHHLPLRIFL 180

Query: 252 RTQGITTPITVASVCSALLHPPINYFLVTYLKLGVEGVALSLAWNTLNLNVGLMIYLALS 311
           RTQGITTPITVAS+CSA+LHPPINYFLVTY+KLGVEGVALSLAWNTLNLNVGLMIYLALS
Sbjct: 181 RTQGITTPITVASICSAVLHPPINYFLVTYMKLGVEGVALSLAWNTLNLNVGLMIYLALS 240

Query: 312 SKPLKPWHGVTILSTFQGWQPLLSLAVPSAVSVCLEWWWYEIMLFLCGLLNHPQNTVAAM 371
           SKPLKPWHGVTILSTFQGWQPLLSLAVPSAVSVCLEWWWYEIMLFLCGLL++PQNTVAAM
Sbjct: 241 SKPLKPWHGVTILSTFQGWQPLLSLAVPSAVSVCLEWWWYEIMLFLCGLLSNPQNTVAAM 300

Query: 372 GILIQTTGMLYIVPFSLSAGITTRIGHALGAGEPIRAQWTAVIGLSTGLAFGLTAFLFMT 431
           GILIQTTGMLYIVPFSLSAGITTRIGHALGAG+P+RAQWTAVIGLS+GLAFGL AF+FMT
Sbjct: 301 GILIQTTGMLYIVPFSLSAGITTRIGHALGAGQPVRAQWTAVIGLSSGLAFGLAAFVFMT 360

Query: 432 SVRSVWGKLYTDEPEILRMISSALPILGLCEISNSPQTVACGVLTGTARPKLGARINLYA 491
           SVRSVWGKLYTDEP+IL+MISSALPILGLCEISNSPQTVACGVLTGTARPKLGARINLYA
Sbjct: 361 SVRSVWGKLYTDEPQILQMISSALPILGLCEISNSPQTVACGVLTGTARPKLGARINLYA 420

Query: 492 FYFIGLPVAVLATFTLKTGFLGLWFGLMTAQISCLCMLVRTLLRTDWIQQSARAVELAAT 551
           FYFIGLPVAVLATFT  TGFLGLWFGLMTAQISCLCMLVRTLLRTDW+QQS RAVELA T
Sbjct: 421 FYFIGLPVAVLATFTFNTGFLGLWFGLMTAQISCLCMLVRTLLRTDWVQQSTRAVELATT 480

Query: 552 VGEEIAKEEEDVESGLIDDNADL 570
            GEE AKE+EDVESGLIDDNADL
Sbjct: 481 AGEETAKEDEDVESGLIDDNADL 503

BLAST of Lsi03G022720 vs. NCBI nr
Match: XP_016899664.1 (PREDICTED: protein DETOXIFICATION 53 isoform X1 [Cucumis melo])

HSP 1 Score: 890.6 bits (2300), Expect = 7.2e-255
Identity = 460/472 (97.46%), Postives = 464/472 (98.31%), Query Frame = 0

Query: 98  QMKEELKSLARFAGPIIMTSFLIYSRSVVSMLFLGHLGKAELAGGSLALGFGNITGISIL 157
           QMKEELKSLARFAGPIIMTSFLIYSRSVVSMLFLGHLGKAELAGGSLALGFGNITGISIL
Sbjct: 2   QMKEELKSLARFAGPIIMTSFLIYSRSVVSMLFLGHLGKAELAGGSLALGFGNITGISIL 61

Query: 158 RGLSTGMDPICCQAFGAKRWSVLSQTFLKTLCLLLLVSIPISILWLNMEPILLWLGQDPA 217
           RGLSTGMDPICCQAFGAKRWSVLSQTFLKTLCLLLLVSIPISILWLNMEPILLWLGQDPA
Sbjct: 62  RGLSTGMDPICCQAFGAKRWSVLSQTFLKTLCLLLLVSIPISILWLNMEPILLWLGQDPA 121

Query: 218 ITQVAKVYMVFSIPELLAQAHHLPLRIFLRTQGITTPITVASVCSALLHPPINYFLVTYL 277
           ITQVAKVYMVFSIPELLAQAHHLPLRIFLRTQGITTPITVASV SALLHP INYFLVTYL
Sbjct: 122 ITQVAKVYMVFSIPELLAQAHHLPLRIFLRTQGITTPITVASVASALLHPLINYFLVTYL 181

Query: 278 KLGVEGVALSLAWNTLNLNVGLMIYLALSSKPLKPWHGVTILSTFQGWQPLLSLAVPSAV 337
           KLGVEGVALSLAWNTLNLNVGLMIYLALSSKPLKPWHGVTI STFQGWQPLLSLA+PSAV
Sbjct: 182 KLGVEGVALSLAWNTLNLNVGLMIYLALSSKPLKPWHGVTIPSTFQGWQPLLSLAIPSAV 241

Query: 338 SVCLEWWWYEIMLFLCGLLNHPQNTVAAMGILIQTTGMLYIVPFSLSAGITTRIGHALGA 397
           SVCLEWWWYEIMLFLCGLLN+PQNTVAAMGILIQTTGMLYIVPFSLSAGITTRIGHALGA
Sbjct: 242 SVCLEWWWYEIMLFLCGLLNNPQNTVAAMGILIQTTGMLYIVPFSLSAGITTRIGHALGA 301

Query: 398 GEPIRAQWTAVIGLSTGLAFGLTAFLFMTSVRSVWGKLYTDEPEILRMISSALPILGLCE 457
           GEPIRAQWTA+IGLSTG AFGLTAFLFMTSVRSVWGKLYTDEPEILRMISSALPILGLCE
Sbjct: 302 GEPIRAQWTAIIGLSTGFAFGLTAFLFMTSVRSVWGKLYTDEPEILRMISSALPILGLCE 361

Query: 458 ISNSPQTVACGVLTGTARPKLGARINLYAFYFIGLPVAVLATFTLKTGFLGLWFGLMTAQ 517
           ISNSPQTVACGVLTGTARPKLGARINLYAFYFIGLPVAVLATFTLKTGFLGLWFGLMTAQ
Sbjct: 362 ISNSPQTVACGVLTGTARPKLGARINLYAFYFIGLPVAVLATFTLKTGFLGLWFGLMTAQ 421

Query: 518 ISCLCMLVRTLLRTDWIQQSARAVELAATVGEEIAKEEEDVESG-LIDDNAD 569
           ISCLCMLVRTLLRTDWIQQS RAVELAA VGEE AKEEEDVE+G LIDDNAD
Sbjct: 422 ISCLCMLVRTLLRTDWIQQSVRAVELAAAVGEETAKEEEDVETGALIDDNAD 473

BLAST of Lsi03G022720 vs. NCBI nr
Match: XP_022982583.1 (protein DETOXIFICATION 53 [Cucurbita maxima] >XP_022982584.1 protein DETOXIFICATION 53 [Cucurbita maxima] >XP_022982586.1 protein DETOXIFICATION 53 [Cucurbita maxima])

HSP 1 Score: 890.2 bits (2299), Expect = 9.4e-255
Identity = 449/487 (92.20%), Postives = 469/487 (96.30%), Query Frame = 0

Query: 80  FFRRLLPLNALPQCLPPQQMKEELKSLARFAGPIIMTSFLIYSRSVVSMLFLGHLGKAEL 139
           FFRRL PLNALPQ LPP QMKEELKSLARF+GPIIMTSFL+YSRSVVSMLFLGHLGKAEL
Sbjct: 43  FFRRLFPLNALPQGLPPHQMKEELKSLARFSGPIIMTSFLLYSRSVVSMLFLGHLGKAEL 102

Query: 140 AGGSLALGFGNITGISILRGLSTGMDPICCQAFGAKRWSVLSQTFLKTLCLLLLVSIPIS 199
           AGGSLALGFGNITGISILRGLSTGMDPICCQAFGAKRWSVLSQTFLKTLCLLLLVS+PIS
Sbjct: 103 AGGSLALGFGNITGISILRGLSTGMDPICCQAFGAKRWSVLSQTFLKTLCLLLLVSLPIS 162

Query: 200 ILWLNMEPILLWLGQDPAITQVAKVYMVFSIPELLAQAHHLPLRIFLRTQGITTPITVAS 259
           +LWLNMEPILLWLGQDPAITQVAKVYM+FSIPELLAQAHHLPLRIFLRTQGITTPITVAS
Sbjct: 163 VLWLNMEPILLWLGQDPAITQVAKVYMLFSIPELLAQAHHLPLRIFLRTQGITTPITVAS 222

Query: 260 VCSALLHPPINYFLVTYLKLGVEGVALSLAWNTLNLNVGLMIYLALSSKPLKPWHGVTIL 319
           +CSALLHPPINYFLV+YL LGVEGVALSLAWNT NLN+GLM+YL LSSKPLKPWHGVTIL
Sbjct: 223 ICSALLHPPINYFLVSYLNLGVEGVALSLAWNTFNLNLGLMLYLVLSSKPLKPWHGVTIL 282

Query: 320 STFQGWQPLLSLAVPSAVSVCLEWWWYEIMLFLCGLLNHPQNTVAAMGILIQTTGMLYIV 379
           STFQGWQPLLSLAVPSAVSVCLEWWWYEIMLFLCGLL++PQNTVAAMGILIQTTGMLYIV
Sbjct: 283 STFQGWQPLLSLAVPSAVSVCLEWWWYEIMLFLCGLLSNPQNTVAAMGILIQTTGMLYIV 342

Query: 380 PFSLSAGITTRIGHALGAGEPIRAQWTAVIGLSTGLAFGLTAFLFMTSVRSVWGKLYTDE 439
           PFSLSAGITTR+G ALGAGEPIRAQWTAVIGLSTGLAFG+TAF FMTSVRSVWGKLYTDE
Sbjct: 343 PFSLSAGITTRVGQALGAGEPIRAQWTAVIGLSTGLAFGVTAFFFMTSVRSVWGKLYTDE 402

Query: 440 PEILRMISSALPILGLCEISNSPQTVACGVLTGTARPKLGARINLYAFYFIGLPVAVLAT 499
           P+IL MISSALP+LG+CEISNSPQTVACGVLTGTARPKLGARINLYAFYFIGLPVAV+AT
Sbjct: 403 PQILHMISSALPVLGVCEISNSPQTVACGVLTGTARPKLGARINLYAFYFIGLPVAVVAT 462

Query: 500 FTLKTGFLGLWFGLMTAQISCLCMLVRTLLRTDWIQQSARAVELAATVGEEIAKEEEDVE 559
           FTLK GFLGLW GLMTAQISCLCMLV TLLRTDWIQQSARAVE+AA VGEE  KEEE VE
Sbjct: 463 FTLKAGFLGLWIGLMTAQISCLCMLVHTLLRTDWIQQSARAVEMAAIVGEESGKEEEGVE 522

Query: 560 SGLIDDN 567
            GL++ N
Sbjct: 523 CGLMEGN 529

BLAST of Lsi03G022720 vs. TAIR 10
Match: AT2G38510.1 (MATE efflux family protein )

HSP 1 Score: 612.5 bits (1578), Expect = 3.5e-175
Identity = 298/469 (63.54%), Postives = 381/469 (81.24%), Query Frame = 0

Query: 98  QMKEELKSLARFAGPIIMTSFLIYSRSVVSMLFLGHLGKAELAGGSLALGFGNITGISIL 157
           Q+ EE+ SL + A PI+MTS LI+SRS++SM FL HLGK ELAGG+LA+GFGNITG+S+L
Sbjct: 2   QVGEEMASLTKIACPIVMTSLLIFSRSIISMWFLSHLGKVELAGGALAMGFGNITGVSVL 61

Query: 158 RGLSTGMDPICCQAFGAKRWSVLSQTFLKTLCLLLLVSIPISILWLNMEPILLWLGQDPA 217
           +GLS GMDPIC QAFGAKRW+VLS TF K  CLL++VS+PI++ WLN+EPI L LGQDP 
Sbjct: 62  KGLSVGMDPICGQAFGAKRWTVLSHTFQKMFCLLIVVSVPIAVTWLNIEPIFLRLGQDPD 121

Query: 218 ITQVAKVYMVFSIPELLAQAHHLPLRIFLRTQGITTPITVASVCSALLHPPINYFLVTYL 277
           IT+VAK YM+F +PELLAQA   PLR FLRTQG+T+P+T++++ S LLHP  NY  V  +
Sbjct: 122 ITKVAKTYMLFFVPELLAQAMLHPLRTFLRTQGLTSPLTISAIVSILLHPLFNYVFVVRM 181

Query: 278 KLGVEGVALSLAWNTLNLNVGLMIYLALSSKPLKPWHGVTILSTFQGWQPLLSLAVPSAV 337
           +LGV+GVA+++A+NT+N++VGL++Y   S   +KPW G+ + S F+GW PLLSLA PSA+
Sbjct: 182 RLGVKGVAIAMAFNTMNIDVGLLVYTCFSDSLIKPWEGLALRSLFRGWWPLLSLAAPSAI 241

Query: 338 SVCLEWWWYEIMLFLCGLLNHPQNTVAAMGILIQTTGMLYIVPFSLSAGITTRIGHALGA 397
           SVCLE+WWYEIMLFLCGLL +P+ +VAAMGILIQTTG+LY+VPF++S+ I TR+GHALG 
Sbjct: 242 SVCLEYWWYEIMLFLCGLLGNPKASVAAMGILIQTTGILYVVPFAISSAIATRVGHALGG 301

Query: 398 GEPIRAQWTAVIGLSTGLAFGLTAFLFMTSVRSVWGKLYTDEPEILRMISSALPILGLCE 457
           G+P RAQ T VIGL   +A+GL A +F+T++RSVWGK++TDEPEIL +IS+ALPILGLCE
Sbjct: 302 GQPTRAQCTTVIGLILAVAYGLAAAVFVTALRSVWGKMFTDEPEILGLISAALPILGLCE 361

Query: 458 ISNSPQTVACGVLTGTARPKLGARINLYAFYFIGLPVAVLATFTLKTGFLGLWFGLMTAQ 517
           I NSPQT ACGVLTGTARPK GAR+NL AFY +GLPVAV  TF  K GF GLWFGL++AQ
Sbjct: 362 IGNSPQTAACGVLTGTARPKDGARVNLCAFYIVGLPVAVTTTFGFKVGFRGLWFGLLSAQ 421

Query: 518 ISCLCMLVRTLLRTDWIQQSARAVELAATVGEEIAKEEEDVESGLIDDN 567
           ++CL M++ TL+RTDW  Q  RA EL +   ++   E+E V + + DD+
Sbjct: 422 MTCLVMMLYTLIRTDWSHQVKRAEELTSAAADKSHSEDETVHAEVQDDD 470

BLAST of Lsi03G022720 vs. TAIR 10
Match: AT1G58340.1 (MATE efflux family protein )

HSP 1 Score: 465.7 bits (1197), Expect = 5.4e-131
Identity = 236/445 (53.03%), Postives = 318/445 (71.46%), Query Frame = 0

Query: 101 EELKSLARFAGPIIMTSFLIYSRSVVSMLFLGHLGKAELAGGSLALGFGNITGISILRGL 160
           EE+K++ + +GP  MT  L+YSR+++SMLFLG+LG+ ELAGGSL++GF NITG S++ GL
Sbjct: 57  EEVKAIGKISGPTAMTGLLMYSRAMISMLFLGYLGELELAGGSLSIGFANITGYSVISGL 116

Query: 161 STGMDPICCQAFGAKRWSVLSQTFLKTLCLLLLVSIPISILWLNMEPILLWLGQDPAITQ 220
           S GM+PIC QA+GAK+  +L  T  +T+ LLL  S+PIS  WLNM  ILLW GQD  I+ 
Sbjct: 117 SMGMEPICGQAYGAKQMKLLGLTLQRTVLLLLSCSVPISFSWLNMRRILLWCGQDEEISS 176

Query: 221 VAKVYMVFSIPELLAQAHHLPLRIFLRTQGITTPITVASVCSALLHPPINYFLVTYLKLG 280
           VA+ +++F+IP+L   +   PLRI+LRTQ IT P+T ++  S LLH P+NY LV  L++G
Sbjct: 177 VAQQFLLFAIPDLFLLSLLHPLRIYLRTQNITLPVTYSTAVSVLLHVPLNYLLVVKLEMG 236

Query: 281 VEGVALSLAWNTLNLNVGLMIYLALSSKPLKPWHGVTILSTFQGWQPLLSLAVPSAVSVC 340
           V GVA+++    LNL V L  ++  +S     W  +TI  + +GW  LLSLA+P+ VSVC
Sbjct: 237 VAGVAIAMVLTNLNLVVLLSSFVYFTSVHSDTWVPITI-DSLKGWSALLSLAIPTCVSVC 296

Query: 341 LEWWWYEIMLFLCGLLNHPQNTVAAMGILIQTTGMLYIVPFSLSAGITTRIGHALGAGEP 400
           LEWWWYE M+ LCGLL +P+ TVA+MGILIQTT ++Y+ P SLS G++TRI + LGA  P
Sbjct: 297 LEWWWYEFMIILCGLLANPRATVASMGILIQTTALVYVFPSSLSLGVSTRISNELGAKRP 356

Query: 401 IRAQWTAVIGLSTGLAFGLTAFLFMTSVRSVWGKLYTDEPEILRMISSALPILGLCEISN 460
            +A+ + +I L   +A GL A +F   VR  WG+L+T + EIL++ S ALPI+GLCE+ N
Sbjct: 357 AKARVSMIISLFCAIALGLMAMVFAVLVRHHWGRLFTTDAEILQLTSIALPIVGLCELGN 416

Query: 461 SPQTVACGVLTGTARPKLGARINLYAFYFIGLPVAVLATFTLKTGFLGLWFGLMTAQISC 520
            PQT  CGVL G ARP LGA INL +FYF+G+PVA+L  F  K GF GLWFGL+ AQ +C
Sbjct: 417 CPQTTGCGVLRGCARPTLGANINLGSFYFVGMPVAILFGFVFKQGFPGLWFGLLAAQATC 476

Query: 521 LCMLVRTLLRTDWIQQSARAVELAA 546
             +++  LLRTDW  Q+ RA EL +
Sbjct: 477 ASLMLCALLRTDWKVQAERAEELTS 500

BLAST of Lsi03G022720 vs. TAIR 10
Match: AT4G23030.1 (MATE efflux family protein )

HSP 1 Score: 459.9 bits (1182), Expect = 2.9e-129
Identity = 244/470 (51.91%), Postives = 327/470 (69.57%), Query Frame = 0

Query: 101 EELKSLARFAGPIIMTSFLIYSRSVVSMLFLGHLGK-AELAGGSLALGFGNITGISILRG 160
           +E KS+A+ + P+I+T  L+YSRS++SMLFLG L   + L+GGSLALGF NITG S+L G
Sbjct: 31  QEAKSIAKISLPLILTGLLLYSRSMISMLFLGRLNDLSALSGGSLALGFANITGYSLLSG 90

Query: 161 LSTGMDPICCQAFGAKRWSVLSQTFLKTLCLLLLVSIPISILWLNMEPILLWLGQDPAIT 220
           LS GM+PIC QAFGAKR+ +L     +T  LLLL S+PISILWLN++ ILL+ GQD  I+
Sbjct: 91  LSIGMEPICVQAFGAKRFKLLGLALQRTTLLLLLCSLPISILWLNIKKILLFFGQDEEIS 150

Query: 221 QVAKVYMVFSIPELLAQAHHLPLRIFLRTQGITTPITVASVCSALLHPPINYFLVTYLKL 280
             A+++++FS+P+L+ Q+   P+RI+LR+Q IT P+T ++  + LLH PINY LV+ L L
Sbjct: 151 NQAEIFILFSLPDLILQSFLHPIRIYLRSQSITLPLTYSAFFAVLLHIPINYLLVSSLGL 210

Query: 281 GVEGVALSLAWNTLNLNVGLMIYLALSSKPLKPWHGVTILSTFQGWQPLLSLAVPSAVSV 340
           G++GVAL   W  +NL   L+IY+  S    K W G + +  F+GW+ L+ LA+PS VSV
Sbjct: 211 GLKGVALGAIWTNVNLLGFLIIYIVFSGVYQKTWGGFS-MDCFKGWRSLMKLAIPSCVSV 270

Query: 341 CLEWWWYEIMLFLCGLLNHPQNTVAAMGILIQTTGMLYIVPFSLSAGITTRIGHALGAGE 400
           CLEWWWYEIM+ LCGLL +PQ TVA+MGILIQTT ++YI P SLS  ++TR+G+ LGA +
Sbjct: 271 CLEWWWYEIMILLCGLLLNPQATVASMGILIQTTALIYIFPSSLSISVSTRVGNELGANQ 330

Query: 401 PIRAQWTAVIGLSTGLAFGLTAFLFMTSVRSVWGKLYTDEPEILRMISSALPILGLCEIS 460
           P +A+  A  GLS  L  GL A  F   VR+ W +L+TDE EI+++ S  LPI+GLCE+ 
Sbjct: 331 PDKARIAARTGLSLSLGLGLLAMFFALMVRNCWARLFTDEEEIVKLTSMVLPIIGLCELG 390

Query: 461 NSPQTVACGVLTGTARPKLGARINLYAFYFIGLPVAVLATFTLKTGFLGLWFGLMTAQIS 520
           N PQT  CGVL G+ARPKLGA INL  FYF+G+PVAV  +F     F GLW GL  AQ S
Sbjct: 391 NCPQTTLCGVLRGSARPKLGANINLCCFYFVGMPVAVWLSFFSGFDFKGLWLGLFAAQGS 450

Query: 521 CLCMLVRTLLRTDWIQQSARAVELAATVGEEIAKEEEDVESGLIDDNADL 570
           CL  ++  L RTDW  +  RA EL   +      +E+D  +  + D+ D+
Sbjct: 451 CLISMLVVLARTDWEVEVHRAKEL---MTRSCDGDEDDGNTPFLLDSLDI 496

BLAST of Lsi03G022720 vs. TAIR 10
Match: AT4G29140.1 (MATE efflux family protein )

HSP 1 Score: 424.5 bits (1090), Expect = 1.4e-118
Identity = 230/469 (49.04%), Postives = 310/469 (66.10%), Query Frame = 0

Query: 102 ELKSLARFAGPIIMTSFLIYSRSVVSMLFLGHLGKAELAGGSLALGFGNITGISILRGLS 161
           E KSL   A PI +T+ ++Y RS VSM FLG LG  ELA GSLA+ F NITG S+L GL+
Sbjct: 56  EAKSLFTLAFPIAVTALVLYLRSAVSMFFLGQLGDLELAAGSLAIAFANITGYSVLSGLA 115

Query: 162 TGMDPICCQAFGAKRWSVLSQTFLKTLCLLLLVSIPISILWLNMEPILLWLGQDPAITQV 221
            GM+P+C QAFGA R+ +LS T  +T+  LL+  +PIS+LW N+  I ++L QDP I ++
Sbjct: 116 LGMEPLCSQAFGAHRFKLLSLTLHRTVVFLLVCCVPISVLWFNVGKISVYLHQDPDIAKL 175

Query: 222 AKVYMVFSIPELLAQAHHLPLRIFLRTQGITTPITVASVCSALLHPPINYFLVTYLKLGV 281
           A+ Y++FS+P+LL      P+RI+LR QGI  P+T+AS+  A+ H P N FLV+YL+LG+
Sbjct: 176 AQTYLIFSLPDLLTNTLLHPIRIYLRAQGIIHPVTLASLSGAVFHLPANLFLVSYLRLGL 235

Query: 282 EGVALSLAWNTLNLNVGLMIYLALSSKPLKPWHGVTILSTFQGWQPLLSLAVPSAVSVCL 341
            GVA++ +   + +   L+ Y+  S      W   T    F+GW PLL LA PS VSVCL
Sbjct: 236 TGVAVASSITNIFVVAFLVCYVWASGLHAPTWTDPT-RDCFRGWAPLLRLAGPSCVSVCL 295

Query: 342 EWWWYEIMLFLCGLLNHPQNTVAAMGILIQTTGMLYIVPFSLSAGITTRIGHALGAGEPI 401
           EWWWYEIM+ LCGLL +P++TVAAMG+LIQTT  LY+ P SLS  ++TR+G+ LGA  P 
Sbjct: 296 EWWWYEIMIVLCGLLVNPRSTVAAMGVLIQTTSFLYVFPSSLSFAVSTRVGNELGANRPK 355

Query: 402 RAQWTAVIGLSTGLAFGLTAFLFMTSVRSVWGKLYTDEPEILRMISSALPILGLCEISNS 461
            A+ TA + +      G+ A  F  SVR+ WG+++T + EIL++ ++ALPILGLCEI N 
Sbjct: 356 TAKLTATVAIVFAAVTGIIAAAFAYSVRNAWGRIFTGDKEILQLTAAALPILGLCEIGNC 415

Query: 462 PQTVACGVLTGTARPKLGARINLYAFYFIGLPVAVLATFTLKTGFLGLWFGLMTAQISCL 521
           PQTV CGV+ GTARP   A +NL AFY +G+PVAV   F    GF GLW GL+ AQISC 
Sbjct: 416 PQTVGCGVVRGTARPSTAANVNLGAFYLVGMPVAVGLGFWAGIGFNGLWVGLLAAQISCA 475

Query: 522 CMLVRTLLRTDWIQQSARAVEL--AATVGEEIAKEEEDVESGLIDDNAD 569
            +++  +  TDW  ++ +A  L  A TV  +I K    V +  ID   D
Sbjct: 476 GLMMYVVGTTDWESEAKKAQTLTCAETVENDIIKA---VVASTIDGECD 520

BLAST of Lsi03G022720 vs. TAIR 10
Match: AT5G19700.1 (MATE efflux family protein )

HSP 1 Score: 424.1 bits (1089), Expect = 1.8e-118
Identity = 215/454 (47.36%), Postives = 310/454 (68.28%), Query Frame = 0

Query: 98  QMKEELKSLARFAGPIIMTSFLIYSRSVVSMLFLGHLGKAELAGGSLALGFGNITGISIL 157
           ++K E +SL   A P I+ + ++Y+RS +SMLFLGH+G+ ELAGGSLA+ F NITG S+L
Sbjct: 32  ELKSEARSLFSLAFPTILAALILYARSAISMLFLGHIGELELAGGSLAIAFANITGYSVL 91

Query: 158 RGLSTGMDPICCQAFGAKRWSVLSQTFLKTLCLLLLVSIPISILWLNMEPILLWLGQDPA 217
            GL+ GMDP+C QAFGA R  +LS T  +T+  LL  S+ I  LWLN+  I+++L QDP+
Sbjct: 92  AGLALGMDPLCSQAFGAGRPKLLSLTLQRTVLFLLTSSVVIVALWLNLGKIMIYLHQDPS 151

Query: 218 ITQVAKVYMVFSIPELLAQAHHLPLRIFLRTQGITTPITVASVCSALLHPPINYFLVTYL 277
           I+ +A+ Y++ SIP+LL  +   PLRI+LR QGIT+P+T+A++   + H P+N+FLV+YL
Sbjct: 152 ISSLAQTYILCSIPDLLTNSFLHPLRIYLRAQGITSPLTLATLAGTIFHIPMNFFLVSYL 211

Query: 278 KLGVEGVALSLAWNTLNLNVGLMIYLALSSKPLKPWHGVTILSTFQGWQPLLSLAVPSAV 337
             G  GV+++ A + L + + L+ ++ ++      W   +    F+ W P+++LA+PS +
Sbjct: 212 GWGFMGVSMAAAASNLLVVIFLVAHVWIAGLHQPTWTRPS-SECFKDWGPVVTLAIPSCI 271

Query: 338 SVCLEWWWYEIMLFLCGLLNHPQNTVAAMGILIQTTGMLYIVPFSLSAGITTRIGHALGA 397
            VCLEWWWYEIM  LCGLL  P   VA+MGILIQTT +LYI P SL   ++TR+G+ LG+
Sbjct: 272 GVCLEWWWYEIMTVLCGLLIDPSTPVASMGILIQTTSLLYIFPSSLGLAVSTRVGNELGS 331

Query: 398 GEPIRAQWTAVIGLSTGLAFGLTAFLFMTSVRSVWGKLYTDEPEILRMISSALPILGLCE 457
             P +A+ +A++ +S     GLTA  F   V  VWG ++T++  I+++ ++ALPILGLCE
Sbjct: 332 NRPNKARLSAIVAVSFAGVMGLTASAFAWGVSDVWGWIFTNDVAIIKLTAAALPILGLCE 391

Query: 458 ISNSPQTVACGVLTGTARPKLGARINLYAFYFIGLPVAVLATFTLKTGFLGLWFGLMTAQ 517
           + N PQTV CGV+ GTARP + A INL AFY +G PVAV  TF    GF GLW GL+ AQ
Sbjct: 392 LGNCPQTVGCGVVRGTARPSMAANINLGAFYLVGTPVAVGLTFWAAYGFCGLWVGLLAAQ 451

Query: 518 ISCLCMLVRTLLRTDWIQQSARAVELAATVGEEI 552
           I C  M++  +  TDW +++ RA +L  T G ++
Sbjct: 452 ICCAAMMLYVVATTDWEKEAIRARKLTCTEGVDV 484