Lsi03G021700 (gene) Bottle gourd (USVL1VR-Ls) v1

BLAST of Lsi03G021700 vs. ExPASy Swiss-Prot
Match: Q9M394 (Fructokinase-like 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=FLN1 PE=1 SV=1)

HSP 1 Score: 675.2 bits (1741), Expect = 5.8e-193
Identity = 338/472 (71.61%), Postives = 387/472 (81.99%), Query Frame = 0

Query: 59  LQNSSKSLKLNPPRAS--GNGDDNGVVETPSPSPTRRGRKKGTTSPSSASTATQKKTNRS 118
           +++S  S +   P+AS  G+G  NG     +  P+R+GRKK  T     ST  +K     
Sbjct: 25  VRHSQASRRFLTPKASINGSGITNGAAAETTSKPSRKGRKKKQT-----STVIEK----- 84

Query: 119 KKPQITDKTTEGNSFDQVSEDESIDSIADNYDDGMDTPYEDPPLICCFGAAQKEFVPSIR 178
                          D    D  ++    +YDDG++ PY+DPPL+CCFGA QKEFVP +R
Sbjct: 85  ---------------DNTETDPELNPELADYDDGIEFPYDDPPLVCCFGAVQKEFVPVVR 144

Query: 179 VHDNQMHPDKYSEWKMLQWDPPEFARAPGGSPSNVAISHVRLGGRAAFMGKVGKDDFGDE 238
           VHDN MHPD YS+WKMLQWDPPEF RAPGG PSNVAISHVRLGGRAAFMGKVG+DDFGDE
Sbjct: 145 VHDNPMHPDMYSQWKMLQWDPPEFGRAPGGPPSNVAISHVRLGGRAAFMGKVGEDDFGDE 204

Query: 239 LVLMMNKEKVQTRAVKFDLNSRTACTYMKIKFEDGRLKMETVKEPAEDSLVSSELNLAVL 298
           LVLMMN+E+VQTRAVKFD NS+TACT +KIKF+DG++  ETVKEP EDSL +SELNLAVL
Sbjct: 205 LVLMMNQERVQTRAVKFDENSKTACTRVKIKFKDGKMMAETVKEPPEDSLFASELNLAVL 264

Query: 299 KEARIFHFNSEALLSTAMESTLFKAIQLSKKFGGLIFFDLNLPLPLWKSRDETREYIKKA 358
           KEARIFHFNSE L S  M+STLF AIQ SKKFGGLIFFDLNLPLPLW+SR+ETR+ IKKA
Sbjct: 265 KEARIFHFNSEVLTSPTMQSTLFTAIQWSKKFGGLIFFDLNLPLPLWRSRNETRKLIKKA 324

Query: 359 WNEADIIEVSRQELEFLLDEEYYEKKRNYRPQYYAQTIEQTKNRRDHYHYTPEEISPLWH 418
           WNEA+IIEVS+QELEFLLDE+YYE++RNY PQY+A+  +QTKNRRD+YHYTPEEI  LWH
Sbjct: 325 WNEANIIEVSQQELEFLLDEDYYERRRNYTPQYFAEDFDQTKNRRDYYHYTPEEIKSLWH 384

Query: 419 DRLKLLFVTDGTLRVHYYSPSFHGVAIGTEDVLITPFTCDRTGSGDAVVAGIMRKLTTFP 478
           D+LKLL VTDGTLR+HYY+P+F GV IGTEDVLITPFTCDRTGSGDAVVAGIMRKLTT P
Sbjct: 385 DKLKLLVVTDGTLRLHYYTPTFDGVVIGTEDVLITPFTCDRTGSGDAVVAGIMRKLTTCP 444

Query: 479 EMFENQDVLERQLRFAIAAGIISQWTIGAVRGFPTESATQNLKEQVYVPSMW 529
           EMFE+QDV+ERQLRFA+AAGII+QWTIGAVRGFPTESATQNLKEQVYVPSMW
Sbjct: 445 EMFEDQDVMERQLRFAVAAGIIAQWTIGAVRGFPTESATQNLKEQVYVPSMW 471

BLAST of Lsi03G021700 vs. ExPASy Swiss-Prot
Match: F4I0K2 (Fructokinase-like 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=FLN2 PE=1 SV=2)

HSP 1 Score: 328.9 bits (842), Expect = 1.0e-88
Identity = 188/452 (41.59%), Postives = 266/452 (58.85%), Query Frame = 0

Query: 78  DDNGVVETPSPSPTRRGRKKGTTSPSSASTATQKKTNRSKKPQITDKTTEGN-------- 137
           D   +V    P  TR  +K    S       T+KK  R K+    DK  E +        
Sbjct: 107 DKESIVSALKPKKTRTRKKAAAASSDVEEVKTEKKVRR-KRTVKKDKDVEDDLATIMDAE 166

Query: 138 -----------SFDQVSEDESIDSIADNYDDGMDTPYEDPPLICCFGAAQKEFVPSIRVH 197
                      S D  SE+E ID ++ +  + +   Y  PPL+CCFG+AQ  FVPS R  
Sbjct: 167 VSDVEEALAVESTDTESEEEEID-LSKHEGEDISHTYGWPPLVCCFGSAQHAFVPSGRPA 226

Query: 198 DNQMHPDKYSEWKMLQWDPPEFARAPGGSPSNVAISHVRLGGRAAFMGKVGKDDFGDELV 257
           +  +  + +   +  +W P ++ RAPGG    VAI+   LGG+ AFMGK+G DD+G  ++
Sbjct: 227 NRLLDYELHERMRDAKWAPEKYIRAPGGCAGGVAIALASLGGKVAFMGKLGADDYGQAML 286

Query: 258 LMMNKEKVQTRAVKFDLNSRTACTYMKIKFEDGRLKMETVKEPAEDSLVSSELNLAVLKE 317
             +N  KVQTR+VK D    TAC+ MKI  + GRLK   +K  AEDSL  SE+N+ VLKE
Sbjct: 287 YYLNVCKVQTRSVKIDGKRVTACSTMKIS-KRGRLKSTCIKPCAEDSLSKSEINVDVLKE 346

Query: 318 ARIFHFNSEALLSTAMESTLFKAIQLSKKFGGLIFFDLNLPLPLWKSRDETREYIKKAWN 377
           A++F+F++ +LL   M ST  +AI++SK+ G +IF+DLNLPLPLW S +ET+ +I++ WN
Sbjct: 347 AKMFYFSTHSLLDKKMMSTTIQAIKISKQLGNVIFYDLNLPLPLWHSSEETKSFIQEVWN 406

Query: 378 EADIIEVSRQELEFLLDEEYYEKKRNYRPQYYAQTIEQTKNRRDHYHYTPEEISPLWHDR 437
            AD+IE+++QELEFL   E  E       ++  +  + +K      HY PE +  LWH+ 
Sbjct: 407 LADVIEITKQELEFLCGIEPTE-------EFDTENNDISK----FVHYPPETVEQLWHEN 466

Query: 438 LKLLFVTDGTLRVHYYSPSFHGVAIGTEDVLITPFTCDRTGSGDAVVAGIMRKLTTFPEM 497
           LK+LFVT+GT ++HYY+   +G   G EDV ITPFT D + SGD +VAG++R LT  P++
Sbjct: 467 LKVLFVTNGTSKIHYYTKEHNGAVSGMEDVPITPFTRDMSASGDGIVAGLIRMLTVQPDL 526

Query: 498 FENQDVLERQLRFAIAAGIISQWTIGAVRGFP 511
             N+  LER  R+AI  GII QW +   RG+P
Sbjct: 527 MNNKGYLERTARYAIECGIIDQWLLAQTRGYP 544

BLAST of Lsi03G021700 vs. ExPASy Swiss-Prot
Match: Q42896 (Fructokinase-2 OS=Solanum lycopersicum OX=4081 GN=FRK2 PE=2 SV=2)

HSP 1 Score: 193.0 bits (489), Expect = 8.7e-48
Identity = 113/324 (34.88%), Postives = 174/324 (53.70%), Query Frame = 0

Query: 196 DPPEFARAPGGSPSNVAISHVRLGGRAAFMGKVGKDDFGDELVLMMNKEKVQTRAVKFDL 255
           + P F +APGG+P+NVAI+  RLGG++AF+GK+G D+FG  L  ++    VQ   + FD 
Sbjct: 33  EAPGFLKAPGGAPANVAIAVTRLGGKSAFVGKLGDDEFGHMLAGILKTNGVQAEGINFDK 92

Query: 256 NSRTACTYMKIKFEDGRLKMETVKEPAEDSLVS-SELNLAVLKEARIFHFNSEALLSTAM 315
            +RTA  ++ ++  DG  +    + P+ D L++ +ELNL +++ A++FH+ S +L+    
Sbjct: 93  GARTALAFVTLR-ADGEREFMFYRNPSADMLLTPAELNLDLIRSAKVFHYGSISLIVEPC 152

Query: 316 ESTLFKAIQLSKKFGGLIFFDLNLPLPLWKSRDETREYIKKAWNEADIIEVSRQELEFLL 375
            +   KA++++K+ G L+ +D NL LPLW S +E ++ IK  W+ AD+I+VS  ELEFL 
Sbjct: 153 RAAHMKAMEVAKEAGALLSYDPNLRLPLWPSAEEAKKQIKSIWDSADVIKVSDVELEFLT 212

Query: 376 DEEYYEKKRNYRPQYYAQTIEQTKNRRDHYHYTPEEISPLWHDRLKLLFVTDGTLRVHYY 435
                                   N+ D      E    LWH  LKLL VT G    +YY
Sbjct: 213 G----------------------SNKID-----DESAMSLWHPNLKLLLVTLGEKGCNYY 272

Query: 436 SPSFHGVAIGTEDVLITPFTCDRTGSGDAVVAGIMRKLTTFPEMFENQDVLERQLRFAIA 495
           +  FHG   G         T D TG+GD+ V  ++ K+     + E++  L+  LRF+ A
Sbjct: 273 TKKFHGTVGG-----FHVKTVDTTGAGDSFVGALLTKIVDDQTILEDEARLKEVLRFSCA 323

Query: 496 AGIISQWTIGAVRGFPTESATQNL 519
            G I+    GA+   PT S    L
Sbjct: 333 CGAITTTKKGAIPALPTASEALTL 323

BLAST of Lsi03G021700 vs. ExPASy Swiss-Prot
Match: Q7XJ81 (Fructokinase-2 OS=Solanum habrochaites OX=62890 GN=FRK2 PE=2 SV=1)

HSP 1 Score: 191.0 bits (484), Expect = 3.3e-47
Identity = 113/324 (34.88%), Postives = 173/324 (53.40%), Query Frame = 0

Query: 196 DPPEFARAPGGSPSNVAISHVRLGGRAAFMGKVGKDDFGDELVLMMNKEKVQTRAVKFDL 255
           + P F +APGG+P+NVAI+  RLGGR+AF+GK+G D+FG  L  ++    VQ   + FD 
Sbjct: 33  EAPGFLKAPGGAPANVAIAVTRLGGRSAFVGKLGDDEFGHMLAGILKTNGVQADGINFDK 92

Query: 256 NSRTACTYMKIKFEDGRLKMETVKEPAEDSLVS-SELNLAVLKEARIFHFNSEALLSTAM 315
            +RTA  ++ ++  DG  +    + P+ D L++ +ELNL +++ A++FH+ S +L+    
Sbjct: 93  GARTALAFVTLR-ADGEREFMFYRNPSADMLLTPAELNLDLIRSAKVFHYGSISLIVEPC 152

Query: 316 ESTLFKAIQLSKKFGGLIFFDLNLPLPLWKSRDETREYIKKAWNEADIIEVSRQELEFLL 375
            +   KA++++K+ G L+ +D NL LPLW S +E ++ IK  W+ AD+I+VS  ELEFL 
Sbjct: 153 RAAHMKAMEVAKEAGALLSYDPNLRLPLWPSAEEAKKQIKSIWDSADVIKVSDVELEFLT 212

Query: 376 DEEYYEKKRNYRPQYYAQTIEQTKNRRDHYHYTPEEISPLWHDRLKLLFVTDGTLRVHYY 435
                                   N+ D      E    LWH  LKLL VT G    +YY
Sbjct: 213 G----------------------SNKID-----DESAMSLWHPNLKLLLVTLGEKGCNYY 272

Query: 436 SPSFHGVAIGTEDVLITPFTCDRTGSGDAVVAGIMRKLTTFPEMFENQDVLERQLRFAIA 495
           +  FHG   G         T D TG+GD+ V  ++ K+     +  ++  L+  LRF+ A
Sbjct: 273 TKKFHGTVGG-----FHVKTVDTTGAGDSFVGALLTKIVDDQTILADEARLKEVLRFSCA 323

Query: 496 AGIISQWTIGAVRGFPTESATQNL 519
            G I+    GA+   PT S    L
Sbjct: 333 CGAITTTKKGAIPALPTASEALTL 323

BLAST of Lsi03G021700 vs. ExPASy Swiss-Prot
Match: Q9LNE3 (Probable fructokinase-2 OS=Arabidopsis thaliana OX=3702 GN=At1g06030 PE=2 SV=1)

HSP 1 Score: 190.7 bits (483), Expect = 4.3e-47
Identity = 118/325 (36.31%), Postives = 177/325 (54.46%), Query Frame = 0

Query: 200 FARAPGGSPSNVAISHVRLGGRAAFMGKVGKDDFGDELVLMMNKEKVQTRAVKFDLNSRT 259
           F +APGG+P+NVAI+  RLGGRAAF+GK+G D+FG  L  ++ K  V  + + FD  +RT
Sbjct: 37  FLKAPGGAPANVAIAVSRLGGRAAFVGKLGDDEFGHMLAGILRKNDVDDQGINFDKGART 96

Query: 260 ACTYMKIKFEDGRLKMETVKEPAEDSLV-SSELNLAVLKEARIFHFNSEALLSTAMESTL 319
           A  ++ ++  DG  +    + P+ D L+   ELNL +++ A++FH+ S +L++    S  
Sbjct: 97  ALAFVTLR-SDGEREFMFYRNPSADMLLRPDELNLELIRSAKVFHYGSISLITEPCRSAH 156

Query: 320 FKAIQLSKKFGGLIFFDLNLPLPLWKSRDETREYIKKAWNEADIIEVSRQELEFLLDEEY 379
            KA++++K+ G L+ +D NL  PLW S +E R+ I   W++ADII+VS  ELEFL     
Sbjct: 157 MKAMEVAKEAGALLSYDPNLREPLWPSPEEARKQIMSIWDKADIIKVSDVELEFLTGN-- 216

Query: 380 YEKKRNYRPQYYAQTIEQTKNRRDHYHYTPEEISPLWHDRLKLLFVTDGTLRVHYYSPSF 439
                        +TI+             E    LWH  LKLL VT G     YY+  F
Sbjct: 217 -------------KTIDD------------ETAMSLWHPNLKLLLVTLGENGCRYYTKDF 276

Query: 440 HGVAIGTEDVLITPFTCDRTGSGDAVVAGIMRKLTTFPEMFENQDVLERQLRFAIAAGII 499
           HG ++ T  V       D TG+GD+ V  ++ ++     + E ++ L + LRFA A G I
Sbjct: 277 HG-SVETFHV----DAVDTTGAGDSFVGALLNQIVDDQSVLEEEERLRKVLRFANACGAI 328

Query: 500 SQWTIGAVRGFPTE-SATQNLKEQV 523
           +    GA+   PT+  A   LK QV
Sbjct: 337 TTTKKGAIPALPTDCEALSFLKIQV 328

BLAST of Lsi03G021700 vs. ExPASy TrEMBL
Match: A0A5A7TRC7 (Fructokinase-like 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G001140 PE=4 SV=1)

HSP 1 Score: 931.0 bits (2405), Expect = 2.2e-267
Identity = 471/514 (91.63%), Postives = 485/514 (94.36%), Query Frame = 0

Query: 15  MASFQLLPSTLSPPHLQFSRFLPFYSSNYGAKTLDLSLIFNSNHLQNSSKSLKLNPPRAS 74
           MASF LLPSTL  PH+QFS FLPFYSS Y AK +DLSLIFN  H Q+SSK+LKLNPPRAS
Sbjct: 1   MASFHLLPSTLPLPHVQFSCFLPFYSSTYRAKPIDLSLIFNLRHFQSSSKTLKLNPPRAS 60

Query: 75  GNGDDNGVVETPSPSPTRRGRKKGTTSPSSASTATQKKTNRSKKPQITDKTTEGNSFDQV 134
            NG+ NGVVET  PSPTRRGRKKGT SPSS ST+ QKKT RSKKPQITDKTT  N FD +
Sbjct: 61  TNGEGNGVVET--PSPTRRGRKKGTASPSS-STSAQKKTKRSKKPQITDKTTVANPFDHI 120

Query: 135 SEDESIDSIADNYDDGMDTPYEDPPLICCFGAAQKEFVPSIRVHDNQMHPDKYSEWKMLQ 194
           SEDESIDSIADNYDDGMD PYEDPPLICCFGAAQKEFVPSIRVHDNQM  DKYSEWKMLQ
Sbjct: 121 SEDESIDSIADNYDDGMDIPYEDPPLICCFGAAQKEFVPSIRVHDNQMDADKYSEWKMLQ 180

Query: 195 WDPPEFARAPGGSPSNVAISHVRLGGRAAFMGKVGKDDFGDELVLMMNKEKVQTRAVKFD 254
           WDPPEFARAPGGSPSNVAI+HVRLGGRAAFMGKVGKDDFGDELVLMMNKEKVQTRAVKFD
Sbjct: 181 WDPPEFARAPGGSPSNVAIAHVRLGGRAAFMGKVGKDDFGDELVLMMNKEKVQTRAVKFD 240

Query: 255 LNSRTACTYMKIKFEDGRLKMETVKEPAEDSLVSSELNLAVLKEARIFHFNSEALLSTAM 314
           LNS+TACTYMKIKFEDG+LKMETVKEPAEDSL+SSELNLAVLKEARIFHFNSEALLST +
Sbjct: 241 LNSKTACTYMKIKFEDGKLKMETVKEPAEDSLLSSELNLAVLKEARIFHFNSEALLSTVI 300

Query: 315 ESTLFKAIQLSKKFGGLIFFDLNLPLPLWKSRDETREYIKKAWNEADIIEVSRQELEFLL 374
           E TLFKAIQLSKKFGGLIFFDLNLPLPLWKSR+ETREYIKKAWNEADIIEVSRQELEFLL
Sbjct: 301 EPTLFKAIQLSKKFGGLIFFDLNLPLPLWKSREETREYIKKAWNEADIIEVSRQELEFLL 360

Query: 375 DEEYYEKKRNYRPQYYAQTIEQTKNRRDHYHYTPEEISPLWHDRLKLLFVTDGTLRVHYY 434
           DEEYYEKKRNYRPQYYAQTIEQTKNRRDHYHYTPEEISPLWHD LKLLFVTDGTLR+HYY
Sbjct: 361 DEEYYEKKRNYRPQYYAQTIEQTKNRRDHYHYTPEEISPLWHDHLKLLFVTDGTLRIHYY 420

Query: 435 SPSFHGVAIGTEDVLITPFTCDRTGSGDAVVAGIMRKLTTFPEMFENQDVLERQLRFAIA 494
           SPSFHGVAIGTEDVLITPFTCDRTGSGDAVVAGIMRKLTTFPEM ENQDVLERQLRFAIA
Sbjct: 421 SPSFHGVAIGTEDVLITPFTCDRTGSGDAVVAGIMRKLTTFPEMLENQDVLERQLRFAIA 480

Query: 495 AGIISQWTIGAVRGFPTESATQNLKEQVYVPSMW 529
           AGIISQWTIGAVRGFPTESATQNLKEQVYVPSMW
Sbjct: 481 AGIISQWTIGAVRGFPTESATQNLKEQVYVPSMW 511

BLAST of Lsi03G021700 vs. ExPASy TrEMBL
Match: A0A1S4DUM6 (fructokinase-like 1, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103486334 PE=4 SV=1)

HSP 1 Score: 931.0 bits (2405), Expect = 2.2e-267
Identity = 471/514 (91.63%), Postives = 485/514 (94.36%), Query Frame = 0

Query: 15  MASFQLLPSTLSPPHLQFSRFLPFYSSNYGAKTLDLSLIFNSNHLQNSSKSLKLNPPRAS 74
           MASF LLPSTL  PH+QFS FLPFYSS Y AK +DLSLIFN  H Q+SSK+LKLNPPRAS
Sbjct: 1   MASFHLLPSTLPLPHVQFSCFLPFYSSTYRAKPIDLSLIFNLRHFQSSSKTLKLNPPRAS 60

Query: 75  GNGDDNGVVETPSPSPTRRGRKKGTTSPSSASTATQKKTNRSKKPQITDKTTEGNSFDQV 134
            NG+ NGVVET  PSPTRRGRKKGT SPSS ST+ QKKT RSKKPQITDKTT  N FD +
Sbjct: 61  TNGEGNGVVET--PSPTRRGRKKGTASPSS-STSAQKKTKRSKKPQITDKTTVANPFDHI 120

Query: 135 SEDESIDSIADNYDDGMDTPYEDPPLICCFGAAQKEFVPSIRVHDNQMHPDKYSEWKMLQ 194
           SEDESIDSIADNYDDGMD PYEDPPLICCFGAAQKEFVPSIRVHDNQM  DKYSEWKMLQ
Sbjct: 121 SEDESIDSIADNYDDGMDIPYEDPPLICCFGAAQKEFVPSIRVHDNQMDADKYSEWKMLQ 180

Query: 195 WDPPEFARAPGGSPSNVAISHVRLGGRAAFMGKVGKDDFGDELVLMMNKEKVQTRAVKFD 254
           WDPPEFARAPGGSPSNVAI+HVRLGGRAAFMGKVGKDDFGDELVLMMNKEKVQTRAVKFD
Sbjct: 181 WDPPEFARAPGGSPSNVAIAHVRLGGRAAFMGKVGKDDFGDELVLMMNKEKVQTRAVKFD 240

Query: 255 LNSRTACTYMKIKFEDGRLKMETVKEPAEDSLVSSELNLAVLKEARIFHFNSEALLSTAM 314
           LNS+TACTYMKIKFEDG+LKMETVKEPAEDSL+SSELNLAVLKEARIFHFNSEALLST +
Sbjct: 241 LNSKTACTYMKIKFEDGKLKMETVKEPAEDSLLSSELNLAVLKEARIFHFNSEALLSTVI 300

Query: 315 ESTLFKAIQLSKKFGGLIFFDLNLPLPLWKSRDETREYIKKAWNEADIIEVSRQELEFLL 374
           E TLFKAIQLSKKFGGLIFFDLNLPLPLWKSR+ETREYIKKAWNEADIIEVSRQELEFLL
Sbjct: 301 EPTLFKAIQLSKKFGGLIFFDLNLPLPLWKSREETREYIKKAWNEADIIEVSRQELEFLL 360

Query: 375 DEEYYEKKRNYRPQYYAQTIEQTKNRRDHYHYTPEEISPLWHDRLKLLFVTDGTLRVHYY 434
           DEEYYEKKRNYRPQYYAQTIEQTKNRRDHYHYTPEEISPLWHD LKLLFVTDGTLR+HYY
Sbjct: 361 DEEYYEKKRNYRPQYYAQTIEQTKNRRDHYHYTPEEISPLWHDHLKLLFVTDGTLRIHYY 420

Query: 435 SPSFHGVAIGTEDVLITPFTCDRTGSGDAVVAGIMRKLTTFPEMFENQDVLERQLRFAIA 494
           SPSFHGVAIGTEDVLITPFTCDRTGSGDAVVAGIMRKLTTFPEM ENQDVLERQLRFAIA
Sbjct: 421 SPSFHGVAIGTEDVLITPFTCDRTGSGDAVVAGIMRKLTTFPEMLENQDVLERQLRFAIA 480

Query: 495 AGIISQWTIGAVRGFPTESATQNLKEQVYVPSMW 529
           AGIISQWTIGAVRGFPTESATQNLKEQVYVPSMW
Sbjct: 481 AGIISQWTIGAVRGFPTESATQNLKEQVYVPSMW 511

BLAST of Lsi03G021700 vs. ExPASy TrEMBL
Match: A0A0A0LA03 (PfkB domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G732620 PE=4 SV=1)

HSP 1 Score: 920.2 bits (2377), Expect = 3.8e-264
Identity = 465/514 (90.47%), Postives = 483/514 (93.97%), Query Frame = 0

Query: 15  MASFQLLPSTLSPPHLQFSRFLPFYSSNYGAKTLDLSLIFNSNHLQNSSKSLKLNPPRAS 74
           MASF LLPSTL  PH+QFS FLPFYSSNY AK +DLSLIFN +H Q+ SK+LKLNPPRAS
Sbjct: 1   MASFHLLPSTLPLPHVQFSCFLPFYSSNYRAKPIDLSLIFNFHHSQSLSKTLKLNPPRAS 60

Query: 75  GNGDDNGVVETPSPSPTRRGRKKGTTSPSSASTATQKKTNRSKKPQITDKTTEGNSFDQV 134
            N + NGVVET  P PTRRGRKKGT SPSS ST+ QKKT RSK+PQITD+ T  N F  +
Sbjct: 61  ANDEGNGVVET--PGPTRRGRKKGTASPSS-STSAQKKTKRSKEPQITDRITVANPFVHI 120

Query: 135 SEDESIDSIADNYDDGMDTPYEDPPLICCFGAAQKEFVPSIRVHDNQMHPDKYSEWKMLQ 194
           SEDESIDSIADNYDDGMD PYEDPPLICCFGAAQKEFVPSIRVHDNQMH DKYSEWKMLQ
Sbjct: 121 SEDESIDSIADNYDDGMDIPYEDPPLICCFGAAQKEFVPSIRVHDNQMHHDKYSEWKMLQ 180

Query: 195 WDPPEFARAPGGSPSNVAISHVRLGGRAAFMGKVGKDDFGDELVLMMNKEKVQTRAVKFD 254
           WDPPEFARAPGGSPSNVAI+HVRLGGRAAFMGKVGKDDFGDELVLMMNKEKVQTRAVKFD
Sbjct: 181 WDPPEFARAPGGSPSNVAIAHVRLGGRAAFMGKVGKDDFGDELVLMMNKEKVQTRAVKFD 240

Query: 255 LNSRTACTYMKIKFEDGRLKMETVKEPAEDSLVSSELNLAVLKEARIFHFNSEALLSTAM 314
           LNS+TACTYMKIKFEDG+LK ETVKEPAEDSL+SSELNLAVLKEARIFHFNSE+LLSTA+
Sbjct: 241 LNSKTACTYMKIKFEDGKLKTETVKEPAEDSLLSSELNLAVLKEARIFHFNSESLLSTAI 300

Query: 315 ESTLFKAIQLSKKFGGLIFFDLNLPLPLWKSRDETREYIKKAWNEADIIEVSRQELEFLL 374
           E TLFKAIQLSKKFGGLIFFDLNLPLPLWKSRDETREYIKKAW EADIIEVSRQELEFLL
Sbjct: 301 EPTLFKAIQLSKKFGGLIFFDLNLPLPLWKSRDETREYIKKAWKEADIIEVSRQELEFLL 360

Query: 375 DEEYYEKKRNYRPQYYAQTIEQTKNRRDHYHYTPEEISPLWHDRLKLLFVTDGTLRVHYY 434
           DEEYYEKKRNYRPQYYAQTIEQTKNRRDHYHYTPEE+SPLWHDRLKLLFVTDGTLR+HYY
Sbjct: 361 DEEYYEKKRNYRPQYYAQTIEQTKNRRDHYHYTPEELSPLWHDRLKLLFVTDGTLRIHYY 420

Query: 435 SPSFHGVAIGTEDVLITPFTCDRTGSGDAVVAGIMRKLTTFPEMFENQDVLERQLRFAIA 494
           SPSFHGVAIGTEDVLITPFTCDRTGSGDAVVAGIMRKLTTFPEM ENQDVLERQLRFAIA
Sbjct: 421 SPSFHGVAIGTEDVLITPFTCDRTGSGDAVVAGIMRKLTTFPEMLENQDVLERQLRFAIA 480

Query: 495 AGIISQWTIGAVRGFPTESATQNLKEQVYVPSMW 529
           AGIISQWTIGAVRGFPTESATQNLKEQVYVPSMW
Sbjct: 481 AGIISQWTIGAVRGFPTESATQNLKEQVYVPSMW 511

BLAST of Lsi03G021700 vs. ExPASy TrEMBL
Match: A0A6J1CW73 (fructokinase-like 1, chloroplastic OS=Momordica charantia OX=3673 GN=LOC111015134 PE=4 SV=1)

HSP 1 Score: 885.6 bits (2287), Expect = 1.0e-253
Identity = 452/519 (87.09%), Postives = 474/519 (91.33%), Query Frame = 0

Query: 15  MASFQLLPSTLSPPHLQFSRFLP-----FYSSNYGAKTLDLSLIFNSNHLQNSSKSLKLN 74
           MASFQLLP+T+S PHL FSRFL       Y +      LD  LIFNS ++QNSSKS +LN
Sbjct: 1   MASFQLLPATISLPHLHFSRFLSNPSHCRYQTTELLNPLDSFLIFNSRYVQNSSKSPRLN 60

Query: 75  PPRASGNGDDNGVVETPSPSPTRRGRKKGTTSPSSASTATQKKTNRSKKPQITDKTTEGN 134
           P RAS   D NGVVET   SPTRRGRKKG T  SS+ST TQKKT RSKK  + D+TTEGN
Sbjct: 61  PARASVGDDGNGVVET--ASPTRRGRKKGMTKSSSSSTTTQKKTKRSKKTLVEDETTEGN 120

Query: 135 SFDQVSEDESIDSIADNYDDGMDTPYEDPPLICCFGAAQKEFVPSIRVHDNQMHPDKYSE 194
            F+QVSEDE IDSIA+ YDDGMD PYEDPPLICCFGAAQKEFVPS+RVHDNQMH DKYSE
Sbjct: 121 PFEQVSEDEPIDSIAEKYDDGMDFPYEDPPLICCFGAAQKEFVPSVRVHDNQMHADKYSE 180

Query: 195 WKMLQWDPPEFARAPGGSPSNVAISHVRLGGRAAFMGKVGKDDFGDELVLMMNKEKVQTR 254
           WKMLQWDPPEFARAPGG+PSNVAISHVRLGGRAAFMGKVGKDDFGDELVLMMNKEKVQTR
Sbjct: 181 WKMLQWDPPEFARAPGGAPSNVAISHVRLGGRAAFMGKVGKDDFGDELVLMMNKEKVQTR 240

Query: 255 AVKFDLNSRTACTYMKIKFEDGRLKMETVKEPAEDSLVSSELNLAVLKEARIFHFNSEAL 314
           AVKFDLNSRTACTYMKIKFEDG+LKMETVKE AEDSL+SSELNL+VLKEARIFHFNSEAL
Sbjct: 241 AVKFDLNSRTACTYMKIKFEDGKLKMETVKESAEDSLLSSELNLSVLKEARIFHFNSEAL 300

Query: 315 LSTAMESTLFKAIQLSKKFGGLIFFDLNLPLPLWKSRDETREYIKKAWNEADIIEVSRQE 374
           +STAMESTLFKAIQLSKKFGGLIFFDLNL LPLW+SRDETREYIKKAWNEADIIEVSRQE
Sbjct: 301 MSTAMESTLFKAIQLSKKFGGLIFFDLNLLLPLWRSRDETREYIKKAWNEADIIEVSRQE 360

Query: 375 LEFLLDEEYYEKKRNYRPQYYAQTIEQTKNRRDHYHYTPEEISPLWHDRLKLLFVTDGTL 434
           LEFLLDEEYYEKKRNYRPQYYAQTIEQTKNRRD+YHYTPEEISPLWHDRL+LLFVTDGT+
Sbjct: 361 LEFLLDEEYYEKKRNYRPQYYAQTIEQTKNRRDYYHYTPEEISPLWHDRLRLLFVTDGTM 420

Query: 435 RVHYYSPSFHGVAIGTEDVLITPFTCDRTGSGDAVVAGIMRKLTTFPEMFENQDVLERQL 494
           R+HYYSPSFHGV IGTEDVLITPFTCDRTGSGDAVVAGIMRKLTTFPEMFENQD LERQL
Sbjct: 421 RIHYYSPSFHGVVIGTEDVLITPFTCDRTGSGDAVVAGIMRKLTTFPEMFENQDXLERQL 480

Query: 495 RFAIAAGIISQWTIGAVRGFPTESATQNLKEQVYVPSMW 529
           RFAIAAGIISQWTIGAVRGFPTESATQNLKEQVYVPSMW
Sbjct: 481 RFAIAAGIISQWTIGAVRGFPTESATQNLKEQVYVPSMW 517

BLAST of Lsi03G021700 vs. ExPASy TrEMBL
Match: A0A6J1FBN1 (fructokinase-like 1, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111442595 PE=4 SV=1)

HSP 1 Score: 860.9 bits (2223), Expect = 2.8e-246
Identity = 443/516 (85.85%), Postives = 470/516 (91.09%), Query Frame = 0

Query: 15  MASFQLLPSTLSPPHLQFSRFLPFYSSNYGAKTLDLSLIFNSNHLQNSSKSLKLNPPRAS 74
           MASFQLLP+T S PHLQFSRFL FY S +  KT    LIFNS +LQ+S KSLKL+PP AS
Sbjct: 1   MASFQLLPATFSLPHLQFSRFLHFYPSIFREKT----LIFNSRYLQSSCKSLKLSPPGAS 60

Query: 75  GNGDDNGVVETPSPSPTRRGRKKGTTSPSSASTATQKKTNRSKKPQITDKTTEGNSFDQV 134
            N D N   +T +P+PTRRGRKKGT SPSS+       T RS+K  I D+ T+ N FDQ+
Sbjct: 61  -NADGN--EDTGTPTPTRRGRKKGTKSPSSS-------TKRSRKTLIEDEPTQVNLFDQM 120

Query: 135 S--EDESIDSIADNYDDGMDTPYEDPPLICCFGAAQKEFVPSIRVHDNQMHPDKYSEWKM 194
           S  EDE+IDSIA+NYDDGMD PYEDPPLICCFGAAQKEFVPSIRVHDNQMHPDKYSEWKM
Sbjct: 121 SVDEDEAIDSIAENYDDGMDFPYEDPPLICCFGAAQKEFVPSIRVHDNQMHPDKYSEWKM 180

Query: 195 LQWDPPEFARAPGGSPSNVAISHVRLGGRAAFMGKVGKDDFGDELVLMMNKEKVQTRAVK 254
           LQWDPPEFARAPGGSPSNVAISHVRLGGRAAFMGKVGKDDFGDELVLMMNKEKVQTR VK
Sbjct: 181 LQWDPPEFARAPGGSPSNVAISHVRLGGRAAFMGKVGKDDFGDELVLMMNKEKVQTRGVK 240

Query: 255 FDLNSRTACTYMKIKFEDGRLKMETVKEPAEDSLVSSELNLAVLKEARIFHFNSEALLST 314
           FDLNSRTACTYMKIKF+DG+LKMETVKE AEDSLVSSELNLAVLKEARIFHFNSEALLST
Sbjct: 241 FDLNSRTACTYMKIKFDDGKLKMETVKESAEDSLVSSELNLAVLKEARIFHFNSEALLST 300

Query: 315 AMESTLFKAIQLSKKFGGLIFFDLNLPLPLWKSRDETREYIKKAWNEADIIEVSRQELEF 374
            ME+TLFKAIQLSKKFGGLIFFDLNL LPLW+SRD+TR+YI+KAWN+ADIIEVSRQELEF
Sbjct: 301 TMEATLFKAIQLSKKFGGLIFFDLNLSLPLWRSRDKTRKYIEKAWNQADIIEVSRQELEF 360

Query: 375 LLDEEYYEKKRNYRPQYYAQTIEQTKNRRDHYHYTPEEISPLWHDRLKLLFVTDGTLRVH 434
           LLDEEYYEKKRNYRPQYYAQTIEQTKNRRDHYHY+PEEISPLWHD LKLL VTDGTLR+H
Sbjct: 361 LLDEEYYEKKRNYRPQYYAQTIEQTKNRRDHYHYSPEEISPLWHDHLKLLLVTDGTLRIH 420

Query: 435 YYSPSFHGVAIGTEDVLITPFTCDRTGSGDAVVAGIMRKLTTFPEMFENQDVLERQLRFA 494
           YYSPSFHGV IGTEDVLITPFTCDRTGSGDA+VAGIMRKLTTFPEMFE+QDVLERQLRFA
Sbjct: 421 YYSPSFHGVVIGTEDVLITPFTCDRTGSGDALVAGIMRKLTTFPEMFESQDVLERQLRFA 480

Query: 495 IAAGIISQWTIGAVRGFPTESATQNLKEQVYVPSMW 529
           IAAGIISQWTIGAVRGFPTESATQNLKEQVYVPSMW
Sbjct: 481 IAAGIISQWTIGAVRGFPTESATQNLKEQVYVPSMW 502

BLAST of Lsi03G021700 vs. NCBI nr
Match: XP_038906230.1 (fructokinase-like 1, chloroplastic [Benincasa hispida])

HSP 1 Score: 951.0 bits (2457), Expect = 4.2e-273
Identity = 483/514 (93.97%), Postives = 493/514 (95.91%), Query Frame = 0

Query: 15  MASFQLLPSTLSPPHLQFSRFLPFYSSNYGAKTLDLSLIFNSNHLQNSSKSLKLNPPRAS 74
           MASFQLLPSTLS PH+QFS FLPFYSSNY AKTLDLSLIF S+HLQ+SS+SLK N PRAS
Sbjct: 1   MASFQLLPSTLSLPHVQFSCFLPFYSSNYRAKTLDLSLIFKSHHLQSSSQSLKQNTPRAS 60

Query: 75  GNGDDNGVVETPSPSPTRRGRKKGTTSPSSASTATQKKTNRSKKPQITDKTTEGNSFDQV 134
            NGD NGVVET  PSPTRRGRKKGTTSPSS+S  TQKKT RSKKPQITD+TTEGN FD  
Sbjct: 61  VNGDGNGVVET--PSPTRRGRKKGTTSPSSSS--TQKKTKRSKKPQITDETTEGNPFDHA 120

Query: 135 SEDESIDSIADNYDDGMDTPYEDPPLICCFGAAQKEFVPSIRVHDNQMHPDKYSEWKMLQ 194
           SEDE IDSIADNYDDGMD PYEDPPLICCFGAAQKEFVPSIRVHDNQMHPDKYSEWKMLQ
Sbjct: 121 SEDEPIDSIADNYDDGMDIPYEDPPLICCFGAAQKEFVPSIRVHDNQMHPDKYSEWKMLQ 180

Query: 195 WDPPEFARAPGGSPSNVAISHVRLGGRAAFMGKVGKDDFGDELVLMMNKEKVQTRAVKFD 254
           WDPPEFARAPGGSPSNVAISHVRLGGRAAFMGKVGKDDFGDE+VLMMNKEKVQTRAVKFD
Sbjct: 181 WDPPEFARAPGGSPSNVAISHVRLGGRAAFMGKVGKDDFGDEVVLMMNKEKVQTRAVKFD 240

Query: 255 LNSRTACTYMKIKFEDGRLKMETVKEPAEDSLVSSELNLAVLKEARIFHFNSEALLSTAM 314
           LNSRTACTYMKIKFEDGRLKMETVKEPAEDSLVSSELNLAVLKEARIFHFNSEALLSTAM
Sbjct: 241 LNSRTACTYMKIKFEDGRLKMETVKEPAEDSLVSSELNLAVLKEARIFHFNSEALLSTAM 300

Query: 315 ESTLFKAIQLSKKFGGLIFFDLNLPLPLWKSRDETREYIKKAWNEADIIEVSRQELEFLL 374
           +STLFKAIQLSKKFGGLIFFDLNLPLPLWKSRDETREYIKKAWNEADIIEVSRQELEFLL
Sbjct: 301 KSTLFKAIQLSKKFGGLIFFDLNLPLPLWKSRDETREYIKKAWNEADIIEVSRQELEFLL 360

Query: 375 DEEYYEKKRNYRPQYYAQTIEQTKNRRDHYHYTPEEISPLWHDRLKLLFVTDGTLRVHYY 434
           DEEYYEKKRNYRPQYYAQTIEQTKNRRDHYHYTPEEISPLWHDRLKLLFVTDGTLR+HYY
Sbjct: 361 DEEYYEKKRNYRPQYYAQTIEQTKNRRDHYHYTPEEISPLWHDRLKLLFVTDGTLRIHYY 420

Query: 435 SPSFHGVAIGTEDVLITPFTCDRTGSGDAVVAGIMRKLTTFPEMFENQDVLERQLRFAIA 494
           SPSFHGVAIGTEDVLITPFTCDRTGSGDAVVAGIMRKLTTFPE+  NQD LERQLRFAIA
Sbjct: 421 SPSFHGVAIGTEDVLITPFTCDRTGSGDAVVAGIMRKLTTFPEILVNQDALERQLRFAIA 480

Query: 495 AGIISQWTIGAVRGFPTESATQNLKEQVYVPSMW 529
           AGIISQWTIGAVRGFPTESATQNLKEQVYVPSMW
Sbjct: 481 AGIISQWTIGAVRGFPTESATQNLKEQVYVPSMW 510

BLAST of Lsi03G021700 vs. NCBI nr
Match: XP_016899692.1 (PREDICTED: fructokinase-like 1, chloroplastic [Cucumis melo] >KAA0044141.1 fructokinase-like 1 [Cucumis melo var. makuwa] >TYK24996.1 fructokinase-like 1 [Cucumis melo var. makuwa])

HSP 1 Score: 931.0 bits (2405), Expect = 4.5e-267
Identity = 471/514 (91.63%), Postives = 485/514 (94.36%), Query Frame = 0

Query: 15  MASFQLLPSTLSPPHLQFSRFLPFYSSNYGAKTLDLSLIFNSNHLQNSSKSLKLNPPRAS 74
           MASF LLPSTL  PH+QFS FLPFYSS Y AK +DLSLIFN  H Q+SSK+LKLNPPRAS
Sbjct: 1   MASFHLLPSTLPLPHVQFSCFLPFYSSTYRAKPIDLSLIFNLRHFQSSSKTLKLNPPRAS 60

Query: 75  GNGDDNGVVETPSPSPTRRGRKKGTTSPSSASTATQKKTNRSKKPQITDKTTEGNSFDQV 134
            NG+ NGVVET  PSPTRRGRKKGT SPSS ST+ QKKT RSKKPQITDKTT  N FD +
Sbjct: 61  TNGEGNGVVET--PSPTRRGRKKGTASPSS-STSAQKKTKRSKKPQITDKTTVANPFDHI 120

Query: 135 SEDESIDSIADNYDDGMDTPYEDPPLICCFGAAQKEFVPSIRVHDNQMHPDKYSEWKMLQ 194
           SEDESIDSIADNYDDGMD PYEDPPLICCFGAAQKEFVPSIRVHDNQM  DKYSEWKMLQ
Sbjct: 121 SEDESIDSIADNYDDGMDIPYEDPPLICCFGAAQKEFVPSIRVHDNQMDADKYSEWKMLQ 180

Query: 195 WDPPEFARAPGGSPSNVAISHVRLGGRAAFMGKVGKDDFGDELVLMMNKEKVQTRAVKFD 254
           WDPPEFARAPGGSPSNVAI+HVRLGGRAAFMGKVGKDDFGDELVLMMNKEKVQTRAVKFD
Sbjct: 181 WDPPEFARAPGGSPSNVAIAHVRLGGRAAFMGKVGKDDFGDELVLMMNKEKVQTRAVKFD 240

Query: 255 LNSRTACTYMKIKFEDGRLKMETVKEPAEDSLVSSELNLAVLKEARIFHFNSEALLSTAM 314
           LNS+TACTYMKIKFEDG+LKMETVKEPAEDSL+SSELNLAVLKEARIFHFNSEALLST +
Sbjct: 241 LNSKTACTYMKIKFEDGKLKMETVKEPAEDSLLSSELNLAVLKEARIFHFNSEALLSTVI 300

Query: 315 ESTLFKAIQLSKKFGGLIFFDLNLPLPLWKSRDETREYIKKAWNEADIIEVSRQELEFLL 374
           E TLFKAIQLSKKFGGLIFFDLNLPLPLWKSR+ETREYIKKAWNEADIIEVSRQELEFLL
Sbjct: 301 EPTLFKAIQLSKKFGGLIFFDLNLPLPLWKSREETREYIKKAWNEADIIEVSRQELEFLL 360

Query: 375 DEEYYEKKRNYRPQYYAQTIEQTKNRRDHYHYTPEEISPLWHDRLKLLFVTDGTLRVHYY 434
           DEEYYEKKRNYRPQYYAQTIEQTKNRRDHYHYTPEEISPLWHD LKLLFVTDGTLR+HYY
Sbjct: 361 DEEYYEKKRNYRPQYYAQTIEQTKNRRDHYHYTPEEISPLWHDHLKLLFVTDGTLRIHYY 420

Query: 435 SPSFHGVAIGTEDVLITPFTCDRTGSGDAVVAGIMRKLTTFPEMFENQDVLERQLRFAIA 494
           SPSFHGVAIGTEDVLITPFTCDRTGSGDAVVAGIMRKLTTFPEM ENQDVLERQLRFAIA
Sbjct: 421 SPSFHGVAIGTEDVLITPFTCDRTGSGDAVVAGIMRKLTTFPEMLENQDVLERQLRFAIA 480

Query: 495 AGIISQWTIGAVRGFPTESATQNLKEQVYVPSMW 529
           AGIISQWTIGAVRGFPTESATQNLKEQVYVPSMW
Sbjct: 481 AGIISQWTIGAVRGFPTESATQNLKEQVYVPSMW 511

BLAST of Lsi03G021700 vs. NCBI nr
Match: XP_031737955.1 (fructokinase-like 1, chloroplastic [Cucumis sativus] >KGN58805.1 hypothetical protein Csa_000812 [Cucumis sativus])

HSP 1 Score: 920.2 bits (2377), Expect = 7.9e-264
Identity = 465/514 (90.47%), Postives = 483/514 (93.97%), Query Frame = 0

Query: 15  MASFQLLPSTLSPPHLQFSRFLPFYSSNYGAKTLDLSLIFNSNHLQNSSKSLKLNPPRAS 74
           MASF LLPSTL  PH+QFS FLPFYSSNY AK +DLSLIFN +H Q+ SK+LKLNPPRAS
Sbjct: 1   MASFHLLPSTLPLPHVQFSCFLPFYSSNYRAKPIDLSLIFNFHHSQSLSKTLKLNPPRAS 60

Query: 75  GNGDDNGVVETPSPSPTRRGRKKGTTSPSSASTATQKKTNRSKKPQITDKTTEGNSFDQV 134
            N + NGVVET  P PTRRGRKKGT SPSS ST+ QKKT RSK+PQITD+ T  N F  +
Sbjct: 61  ANDEGNGVVET--PGPTRRGRKKGTASPSS-STSAQKKTKRSKEPQITDRITVANPFVHI 120

Query: 135 SEDESIDSIADNYDDGMDTPYEDPPLICCFGAAQKEFVPSIRVHDNQMHPDKYSEWKMLQ 194
           SEDESIDSIADNYDDGMD PYEDPPLICCFGAAQKEFVPSIRVHDNQMH DKYSEWKMLQ
Sbjct: 121 SEDESIDSIADNYDDGMDIPYEDPPLICCFGAAQKEFVPSIRVHDNQMHHDKYSEWKMLQ 180

Query: 195 WDPPEFARAPGGSPSNVAISHVRLGGRAAFMGKVGKDDFGDELVLMMNKEKVQTRAVKFD 254
           WDPPEFARAPGGSPSNVAI+HVRLGGRAAFMGKVGKDDFGDELVLMMNKEKVQTRAVKFD
Sbjct: 181 WDPPEFARAPGGSPSNVAIAHVRLGGRAAFMGKVGKDDFGDELVLMMNKEKVQTRAVKFD 240

Query: 255 LNSRTACTYMKIKFEDGRLKMETVKEPAEDSLVSSELNLAVLKEARIFHFNSEALLSTAM 314
           LNS+TACTYMKIKFEDG+LK ETVKEPAEDSL+SSELNLAVLKEARIFHFNSE+LLSTA+
Sbjct: 241 LNSKTACTYMKIKFEDGKLKTETVKEPAEDSLLSSELNLAVLKEARIFHFNSESLLSTAI 300

Query: 315 ESTLFKAIQLSKKFGGLIFFDLNLPLPLWKSRDETREYIKKAWNEADIIEVSRQELEFLL 374
           E TLFKAIQLSKKFGGLIFFDLNLPLPLWKSRDETREYIKKAW EADIIEVSRQELEFLL
Sbjct: 301 EPTLFKAIQLSKKFGGLIFFDLNLPLPLWKSRDETREYIKKAWKEADIIEVSRQELEFLL 360

Query: 375 DEEYYEKKRNYRPQYYAQTIEQTKNRRDHYHYTPEEISPLWHDRLKLLFVTDGTLRVHYY 434
           DEEYYEKKRNYRPQYYAQTIEQTKNRRDHYHYTPEE+SPLWHDRLKLLFVTDGTLR+HYY
Sbjct: 361 DEEYYEKKRNYRPQYYAQTIEQTKNRRDHYHYTPEELSPLWHDRLKLLFVTDGTLRIHYY 420

Query: 435 SPSFHGVAIGTEDVLITPFTCDRTGSGDAVVAGIMRKLTTFPEMFENQDVLERQLRFAIA 494
           SPSFHGVAIGTEDVLITPFTCDRTGSGDAVVAGIMRKLTTFPEM ENQDVLERQLRFAIA
Sbjct: 421 SPSFHGVAIGTEDVLITPFTCDRTGSGDAVVAGIMRKLTTFPEMLENQDVLERQLRFAIA 480

Query: 495 AGIISQWTIGAVRGFPTESATQNLKEQVYVPSMW 529
           AGIISQWTIGAVRGFPTESATQNLKEQVYVPSMW
Sbjct: 481 AGIISQWTIGAVRGFPTESATQNLKEQVYVPSMW 511

BLAST of Lsi03G021700 vs. NCBI nr
Match: XP_022145754.1 (fructokinase-like 1, chloroplastic [Momordica charantia])

HSP 1 Score: 885.6 bits (2287), Expect = 2.2e-253
Identity = 452/519 (87.09%), Postives = 474/519 (91.33%), Query Frame = 0

Query: 15  MASFQLLPSTLSPPHLQFSRFLP-----FYSSNYGAKTLDLSLIFNSNHLQNSSKSLKLN 74
           MASFQLLP+T+S PHL FSRFL       Y +      LD  LIFNS ++QNSSKS +LN
Sbjct: 1   MASFQLLPATISLPHLHFSRFLSNPSHCRYQTTELLNPLDSFLIFNSRYVQNSSKSPRLN 60

Query: 75  PPRASGNGDDNGVVETPSPSPTRRGRKKGTTSPSSASTATQKKTNRSKKPQITDKTTEGN 134
           P RAS   D NGVVET   SPTRRGRKKG T  SS+ST TQKKT RSKK  + D+TTEGN
Sbjct: 61  PARASVGDDGNGVVET--ASPTRRGRKKGMTKSSSSSTTTQKKTKRSKKTLVEDETTEGN 120

Query: 135 SFDQVSEDESIDSIADNYDDGMDTPYEDPPLICCFGAAQKEFVPSIRVHDNQMHPDKYSE 194
            F+QVSEDE IDSIA+ YDDGMD PYEDPPLICCFGAAQKEFVPS+RVHDNQMH DKYSE
Sbjct: 121 PFEQVSEDEPIDSIAEKYDDGMDFPYEDPPLICCFGAAQKEFVPSVRVHDNQMHADKYSE 180

Query: 195 WKMLQWDPPEFARAPGGSPSNVAISHVRLGGRAAFMGKVGKDDFGDELVLMMNKEKVQTR 254
           WKMLQWDPPEFARAPGG+PSNVAISHVRLGGRAAFMGKVGKDDFGDELVLMMNKEKVQTR
Sbjct: 181 WKMLQWDPPEFARAPGGAPSNVAISHVRLGGRAAFMGKVGKDDFGDELVLMMNKEKVQTR 240

Query: 255 AVKFDLNSRTACTYMKIKFEDGRLKMETVKEPAEDSLVSSELNLAVLKEARIFHFNSEAL 314
           AVKFDLNSRTACTYMKIKFEDG+LKMETVKE AEDSL+SSELNL+VLKEARIFHFNSEAL
Sbjct: 241 AVKFDLNSRTACTYMKIKFEDGKLKMETVKESAEDSLLSSELNLSVLKEARIFHFNSEAL 300

Query: 315 LSTAMESTLFKAIQLSKKFGGLIFFDLNLPLPLWKSRDETREYIKKAWNEADIIEVSRQE 374
           +STAMESTLFKAIQLSKKFGGLIFFDLNL LPLW+SRDETREYIKKAWNEADIIEVSRQE
Sbjct: 301 MSTAMESTLFKAIQLSKKFGGLIFFDLNLLLPLWRSRDETREYIKKAWNEADIIEVSRQE 360

Query: 375 LEFLLDEEYYEKKRNYRPQYYAQTIEQTKNRRDHYHYTPEEISPLWHDRLKLLFVTDGTL 434
           LEFLLDEEYYEKKRNYRPQYYAQTIEQTKNRRD+YHYTPEEISPLWHDRL+LLFVTDGT+
Sbjct: 361 LEFLLDEEYYEKKRNYRPQYYAQTIEQTKNRRDYYHYTPEEISPLWHDRLRLLFVTDGTM 420

Query: 435 RVHYYSPSFHGVAIGTEDVLITPFTCDRTGSGDAVVAGIMRKLTTFPEMFENQDVLERQL 494
           R+HYYSPSFHGV IGTEDVLITPFTCDRTGSGDAVVAGIMRKLTTFPEMFENQD LERQL
Sbjct: 421 RIHYYSPSFHGVVIGTEDVLITPFTCDRTGSGDAVVAGIMRKLTTFPEMFENQDXLERQL 480

Query: 495 RFAIAAGIISQWTIGAVRGFPTESATQNLKEQVYVPSMW 529
           RFAIAAGIISQWTIGAVRGFPTESATQNLKEQVYVPSMW
Sbjct: 481 RFAIAAGIISQWTIGAVRGFPTESATQNLKEQVYVPSMW 517

BLAST of Lsi03G021700 vs. NCBI nr
Match: KAG7027918.1 (Fructokinase-like 1, chloroplastic, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 868.6 bits (2243), Expect = 2.7e-248
Identity = 445/516 (86.24%), Postives = 471/516 (91.28%), Query Frame = 0

Query: 15  MASFQLLPSTLSPPHLQFSRFLPFYSSNYGAKTLDLSLIFNSNHLQNSSKSLKLNPPRAS 74
           MASFQLLP+T S PHLQFSRFL FY S +  KT    LIFNS +LQ+S KSLKL+PP AS
Sbjct: 1   MASFQLLPATFSLPHLQFSRFLHFYPSIFREKT----LIFNSRYLQSSCKSLKLSPPGAS 60

Query: 75  GNGDDNGVVETPSPSPTRRGRKKGTTSPSSASTATQKKTNRSKKPQITDKTTEGNSFDQV 134
            N D N   +T +P+PTRRGRKKGT SPSS+       T RS+K  I D+ T+GN FDQ+
Sbjct: 61  -NADGN--EDTGTPTPTRRGRKKGTKSPSSS-------TKRSRKTLIEDEPTQGNLFDQM 120

Query: 135 S--EDESIDSIADNYDDGMDTPYEDPPLICCFGAAQKEFVPSIRVHDNQMHPDKYSEWKM 194
           S  EDE+IDSIA+NYDDGMD PYEDPPLICCFGAAQKEFVPSIRVHDNQMHPDKYSEWKM
Sbjct: 121 SVDEDEAIDSIAENYDDGMDFPYEDPPLICCFGAAQKEFVPSIRVHDNQMHPDKYSEWKM 180

Query: 195 LQWDPPEFARAPGGSPSNVAISHVRLGGRAAFMGKVGKDDFGDELVLMMNKEKVQTRAVK 254
           LQWDPPEFARAPGGSPSNVAISHVRLGGRAAFMGKVGKDDFGDELVLMMNKEKVQTR VK
Sbjct: 181 LQWDPPEFARAPGGSPSNVAISHVRLGGRAAFMGKVGKDDFGDELVLMMNKEKVQTRGVK 240

Query: 255 FDLNSRTACTYMKIKFEDGRLKMETVKEPAEDSLVSSELNLAVLKEARIFHFNSEALLST 314
           FDLNSRTACTYMKIKF+DG+LKMETVKE AEDSLVSSELNLAVLKEARIFHFNSEALLST
Sbjct: 241 FDLNSRTACTYMKIKFDDGKLKMETVKESAEDSLVSSELNLAVLKEARIFHFNSEALLST 300

Query: 315 AMESTLFKAIQLSKKFGGLIFFDLNLPLPLWKSRDETREYIKKAWNEADIIEVSRQELEF 374
            ME+TLF AIQLSKKFGGLIFFDLNLPLPLW+SRDETR+YI+KAWN+ADIIEVSRQELEF
Sbjct: 301 TMEATLFNAIQLSKKFGGLIFFDLNLPLPLWRSRDETRKYIEKAWNQADIIEVSRQELEF 360

Query: 375 LLDEEYYEKKRNYRPQYYAQTIEQTKNRRDHYHYTPEEISPLWHDRLKLLFVTDGTLRVH 434
           LLDEEYYEKKRNYRPQYYAQTIEQTKNRRDHYHY+PEEISPLWHD LKLL +TDGTLR+H
Sbjct: 361 LLDEEYYEKKRNYRPQYYAQTIEQTKNRRDHYHYSPEEISPLWHDHLKLLLITDGTLRIH 420

Query: 435 YYSPSFHGVAIGTEDVLITPFTCDRTGSGDAVVAGIMRKLTTFPEMFENQDVLERQLRFA 494
           YYSPSFHGV IGTEDVLITPFTCDRTGSGDA+VAGIMRKLTTFPEMFENQDVLERQLRFA
Sbjct: 421 YYSPSFHGVVIGTEDVLITPFTCDRTGSGDALVAGIMRKLTTFPEMFENQDVLERQLRFA 480

Query: 495 IAAGIISQWTIGAVRGFPTESATQNLKEQVYVPSMW 529
           IAAGIISQWTIGAVRGFPTESATQNLKEQVYVPSMW
Sbjct: 481 IAAGIISQWTIGAVRGFPTESATQNLKEQVYVPSMW 502

BLAST of Lsi03G021700 vs. TAIR 10
Match: AT3G54090.1 (fructokinase-like 1 )

HSP 1 Score: 675.2 bits (1741), Expect = 4.1e-194
Identity = 338/472 (71.61%), Postives = 387/472 (81.99%), Query Frame = 0

Query: 59  LQNSSKSLKLNPPRAS--GNGDDNGVVETPSPSPTRRGRKKGTTSPSSASTATQKKTNRS 118
           +++S  S +   P+AS  G+G  NG     +  P+R+GRKK  T     ST  +K     
Sbjct: 25  VRHSQASRRFLTPKASINGSGITNGAAAETTSKPSRKGRKKKQT-----STVIEK----- 84

Query: 119 KKPQITDKTTEGNSFDQVSEDESIDSIADNYDDGMDTPYEDPPLICCFGAAQKEFVPSIR 178
                          D    D  ++    +YDDG++ PY+DPPL+CCFGA QKEFVP +R
Sbjct: 85  ---------------DNTETDPELNPELADYDDGIEFPYDDPPLVCCFGAVQKEFVPVVR 144

Query: 179 VHDNQMHPDKYSEWKMLQWDPPEFARAPGGSPSNVAISHVRLGGRAAFMGKVGKDDFGDE 238
           VHDN MHPD YS+WKMLQWDPPEF RAPGG PSNVAISHVRLGGRAAFMGKVG+DDFGDE
Sbjct: 145 VHDNPMHPDMYSQWKMLQWDPPEFGRAPGGPPSNVAISHVRLGGRAAFMGKVGEDDFGDE 204

Query: 239 LVLMMNKEKVQTRAVKFDLNSRTACTYMKIKFEDGRLKMETVKEPAEDSLVSSELNLAVL 298
           LVLMMN+E+VQTRAVKFD NS+TACT +KIKF+DG++  ETVKEP EDSL +SELNLAVL
Sbjct: 205 LVLMMNQERVQTRAVKFDENSKTACTRVKIKFKDGKMMAETVKEPPEDSLFASELNLAVL 264

Query: 299 KEARIFHFNSEALLSTAMESTLFKAIQLSKKFGGLIFFDLNLPLPLWKSRDETREYIKKA 358
           KEARIFHFNSE L S  M+STLF AIQ SKKFGGLIFFDLNLPLPLW+SR+ETR+ IKKA
Sbjct: 265 KEARIFHFNSEVLTSPTMQSTLFTAIQWSKKFGGLIFFDLNLPLPLWRSRNETRKLIKKA 324

Query: 359 WNEADIIEVSRQELEFLLDEEYYEKKRNYRPQYYAQTIEQTKNRRDHYHYTPEEISPLWH 418
           WNEA+IIEVS+QELEFLLDE+YYE++RNY PQY+A+  +QTKNRRD+YHYTPEEI  LWH
Sbjct: 325 WNEANIIEVSQQELEFLLDEDYYERRRNYTPQYFAEDFDQTKNRRDYYHYTPEEIKSLWH 384

Query: 419 DRLKLLFVTDGTLRVHYYSPSFHGVAIGTEDVLITPFTCDRTGSGDAVVAGIMRKLTTFP 478
           D+LKLL VTDGTLR+HYY+P+F GV IGTEDVLITPFTCDRTGSGDAVVAGIMRKLTT P
Sbjct: 385 DKLKLLVVTDGTLRLHYYTPTFDGVVIGTEDVLITPFTCDRTGSGDAVVAGIMRKLTTCP 444

Query: 479 EMFENQDVLERQLRFAIAAGIISQWTIGAVRGFPTESATQNLKEQVYVPSMW 529
           EMFE+QDV+ERQLRFA+AAGII+QWTIGAVRGFPTESATQNLKEQVYVPSMW
Sbjct: 445 EMFEDQDVMERQLRFAVAAGIIAQWTIGAVRGFPTESATQNLKEQVYVPSMW 471

BLAST of Lsi03G021700 vs. TAIR 10
Match: AT1G69200.1 (fructokinase-like 2 )

HSP 1 Score: 328.9 bits (842), Expect = 7.3e-90
Identity = 188/452 (41.59%), Postives = 266/452 (58.85%), Query Frame = 0

Query: 78  DDNGVVETPSPSPTRRGRKKGTTSPSSASTATQKKTNRSKKPQITDKTTEGN-------- 137
           D   +V    P  TR  +K    S       T+KK  R K+    DK  E +        
Sbjct: 109 DKESIVSALKPKKTRTRKKAAAASSDVEEVKTEKKVRR-KRTVKKDKDVEDDLATIMDAE 168

Query: 138 -----------SFDQVSEDESIDSIADNYDDGMDTPYEDPPLICCFGAAQKEFVPSIRVH 197
                      S D  SE+E ID ++ +  + +   Y  PPL+CCFG+AQ  FVPS R  
Sbjct: 169 VSDVEEALAVESTDTESEEEEID-LSKHEGEDISHTYGWPPLVCCFGSAQHAFVPSGRPA 228

Query: 198 DNQMHPDKYSEWKMLQWDPPEFARAPGGSPSNVAISHVRLGGRAAFMGKVGKDDFGDELV 257
           +  +  + +   +  +W P ++ RAPGG    VAI+   LGG+ AFMGK+G DD+G  ++
Sbjct: 229 NRLLDYELHERMRDAKWAPEKYIRAPGGCAGGVAIALASLGGKVAFMGKLGADDYGQAML 288

Query: 258 LMMNKEKVQTRAVKFDLNSRTACTYMKIKFEDGRLKMETVKEPAEDSLVSSELNLAVLKE 317
             +N  KVQTR+VK D    TAC+ MKI  + GRLK   +K  AEDSL  SE+N+ VLKE
Sbjct: 289 YYLNVCKVQTRSVKIDGKRVTACSTMKIS-KRGRLKSTCIKPCAEDSLSKSEINVDVLKE 348

Query: 318 ARIFHFNSEALLSTAMESTLFKAIQLSKKFGGLIFFDLNLPLPLWKSRDETREYIKKAWN 377
           A++F+F++ +LL   M ST  +AI++SK+ G +IF+DLNLPLPLW S +ET+ +I++ WN
Sbjct: 349 AKMFYFSTHSLLDKKMMSTTIQAIKISKQLGNVIFYDLNLPLPLWHSSEETKSFIQEVWN 408

Query: 378 EADIIEVSRQELEFLLDEEYYEKKRNYRPQYYAQTIEQTKNRRDHYHYTPEEISPLWHDR 437
            AD+IE+++QELEFL   E  E       ++  +  + +K      HY PE +  LWH+ 
Sbjct: 409 LADVIEITKQELEFLCGIEPTE-------EFDTENNDISK----FVHYPPETVEQLWHEN 468

Query: 438 LKLLFVTDGTLRVHYYSPSFHGVAIGTEDVLITPFTCDRTGSGDAVVAGIMRKLTTFPEM 497
           LK+LFVT+GT ++HYY+   +G   G EDV ITPFT D + SGD +VAG++R LT  P++
Sbjct: 469 LKVLFVTNGTSKIHYYTKEHNGAVSGMEDVPITPFTRDMSASGDGIVAGLIRMLTVQPDL 528

Query: 498 FENQDVLERQLRFAIAAGIISQWTIGAVRGFP 511
             N+  LER  R+AI  GII QW +   RG+P
Sbjct: 529 MNNKGYLERTARYAIECGIIDQWLLAQTRGYP 546

BLAST of Lsi03G021700 vs. TAIR 10
Match: AT1G06030.1 (pfkB-like carbohydrate kinase family protein )

HSP 1 Score: 190.7 bits (483), Expect = 3.1e-48
Identity = 118/325 (36.31%), Postives = 177/325 (54.46%), Query Frame = 0

Query: 200 FARAPGGSPSNVAISHVRLGGRAAFMGKVGKDDFGDELVLMMNKEKVQTRAVKFDLNSRT 259
           F +APGG+P+NVAI+  RLGGRAAF+GK+G D+FG  L  ++ K  V  + + FD  +RT
Sbjct: 37  FLKAPGGAPANVAIAVSRLGGRAAFVGKLGDDEFGHMLAGILRKNDVDDQGINFDKGART 96

Query: 260 ACTYMKIKFEDGRLKMETVKEPAEDSLV-SSELNLAVLKEARIFHFNSEALLSTAMESTL 319
           A  ++ ++  DG  +    + P+ D L+   ELNL +++ A++FH+ S +L++    S  
Sbjct: 97  ALAFVTLR-SDGEREFMFYRNPSADMLLRPDELNLELIRSAKVFHYGSISLITEPCRSAH 156

Query: 320 FKAIQLSKKFGGLIFFDLNLPLPLWKSRDETREYIKKAWNEADIIEVSRQELEFLLDEEY 379
            KA++++K+ G L+ +D NL  PLW S +E R+ I   W++ADII+VS  ELEFL     
Sbjct: 157 MKAMEVAKEAGALLSYDPNLREPLWPSPEEARKQIMSIWDKADIIKVSDVELEFLTGN-- 216

Query: 380 YEKKRNYRPQYYAQTIEQTKNRRDHYHYTPEEISPLWHDRLKLLFVTDGTLRVHYYSPSF 439
                        +TI+             E    LWH  LKLL VT G     YY+  F
Sbjct: 217 -------------KTIDD------------ETAMSLWHPNLKLLLVTLGENGCRYYTKDF 276

Query: 440 HGVAIGTEDVLITPFTCDRTGSGDAVVAGIMRKLTTFPEMFENQDVLERQLRFAIAAGII 499
           HG ++ T  V       D TG+GD+ V  ++ ++     + E ++ L + LRFA A G I
Sbjct: 277 HG-SVETFHV----DAVDTTGAGDSFVGALLNQIVDDQSVLEEEERLRKVLRFANACGAI 328

Query: 500 SQWTIGAVRGFPTE-SATQNLKEQV 523
           +    GA+   PT+  A   LK QV
Sbjct: 337 TTTKKGAIPALPTDCEALSFLKIQV 328

BLAST of Lsi03G021700 vs. TAIR 10
Match: AT3G59480.1 (pfkB-like carbohydrate kinase family protein )

HSP 1 Score: 190.3 bits (482), Expect = 4.0e-48
Identity = 114/327 (34.86%), Postives = 173/327 (52.91%), Query Frame = 0

Query: 196 DPPEFARAPGGSPSNVAISHVRLGGRAAFMGKVGKDDFGDELVLMMNKEKVQTRAVKFDL 255
           D P F +APGG+P+NVAI+  RLGGRAAF+GK+G D+FG  L  ++ +  V    + FD 
Sbjct: 32  DAPGFIKAPGGAPANVAIAISRLGGRAAFVGKLGDDEFGHMLAGILKQNGVSAEGINFDT 91

Query: 256 NSRTACTYMKIKFEDGRLKMETVKEPAEDSLV-SSELNLAVLKEARIFHFNSEALLSTAM 315
            +RTA  ++ ++  DG  +    + P+ D L+   ELNL V++ A++FH+ S +L+    
Sbjct: 92  GARTALAFVTLR-SDGEREFMFYRNPSADMLLRPDELNLDVIRSAKVFHYGSISLIVEPC 151

Query: 316 ESTLFKAIQLSKKFGGLIFFDLNLPLPLWKSRDETREYIKKAWNEADIIEVSRQELEFLL 375
            S   KA++++K+ G L+ +D NL LPLW S++E ++ I   W++A++I+VS +EL FL 
Sbjct: 152 RSAHLKAMEVAKEAGALLSYDPNLRLPLWPSKEEAQKQILSIWDKAEVIKVSDEELMFLT 211

Query: 376 DEEYYEKKRNYRPQYYAQTIEQTKNRRDHYHYTPEEISPLWHDRLKLLFVTDGTLRVHYY 435
             +  +                            E    LWH  LKLL VT G     YY
Sbjct: 212 GSDKVD---------------------------DETALSLWHSNLKLLLVTLGEKGCRYY 271

Query: 436 SPSFHGVAIGTEDVLITPF---TCDRTGSGDAVVAGIMRKLTTFPEMFENQDVLERQLRF 495
           + SF G         + PF     D TG+GD+ V  ++ K+     + E++  L   LR 
Sbjct: 272 TKSFRG--------SVDPFHVDAVDTTGAGDSFVGALLCKIVDDRAVLEDEARLREVLRL 322

Query: 496 AIAAGIISQWTIGAVRGFPTESATQNL 519
           A A G I+    GA+   PTES  Q+L
Sbjct: 332 ANACGAITTTKKGAIPALPTESEVQSL 322

BLAST of Lsi03G021700 vs. TAIR 10
Match: AT1G06020.1 (pfkB-like carbohydrate kinase family protein )

HSP 1 Score: 189.5 bits (480), Expect = 6.9e-48
Identity = 116/328 (35.37%), Postives = 178/328 (54.27%), Query Frame = 0

Query: 196 DPPEFARAPGGSPSNVAISHVRLGGRAAFMGKVGKDDFGDELVLMMNKEKVQTRAVKFDL 255
           + P F +APGG+P+NVAI+  RLGGRAAF+GK+G DDFG  L  ++ K  V  + + FD 
Sbjct: 32  ESPGFLKAPGGAPANVAIAVSRLGGRAAFVGKLGDDDFGHMLAGILRKNGVDDQGINFDE 91

Query: 256 NSRTACTYMKIKFEDGRLKMETVKEPAEDSLV-SSELNLAVLKEARIFHFNSEALLSTAM 315
            +RTA  ++ ++  DG  +    + P+ D L+   ELNL +++ A++FH+ S +L++   
Sbjct: 92  GARTALAFVTLR-SDGEREFMFYRNPSADMLLRPDELNLELIRSAKVFHYGSISLITEPC 151

Query: 316 ESTLFKAIQLSKKFGGLIFFDLNLPLPLWKSRDETREYIKKAWNEADIIEVSRQELEFLL 375
            S   KA++++K+ G L+ +D NL  PLW S +E R  I   W++ADII+VS  ELEFL 
Sbjct: 152 RSAHMKAMEVAKEAGALLSYDPNLREPLWPSPEEARTQIMSIWDKADIIKVSDVELEFLT 211

Query: 376 DEEYYEKKRNYRPQYYAQTIEQTKNRRDHYHYTPEEISPLWHDRLKLLFVTDGTLRVHYY 435
           + +  + K                               LWH  LKLL VT G     Y+
Sbjct: 212 ENKTMDDK---------------------------TAMSLWHPNLKLLLVTLGEKGCTYF 271

Query: 436 SPSFHGVAIGTEDVLITPFTCDRTGSGDAVVAGIMRKLTTFPEMFENQDVLERQLRFAIA 495
           +  FHG ++ T  V       D TG+GD+ V  +++++     + E++  L + LRFA A
Sbjct: 272 TKKFHG-SVETFHV----DAVDTTGAGDSFVGALLQQIVDDQSVLEDEARLRKVLRFANA 326

Query: 496 AGIISQWTIGAVRGFPTE-SATQNLKEQ 522
            G I+    GA+   PT+  A   LK+Q
Sbjct: 332 CGAITTTKKGAIPALPTDIEALSFLKDQ 326