Lsi03G021600 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi03G021600
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	Nuclear transport factor 2 (NTF2) family protein
Location	chr03: 32733776 .. 32735238 (-)
RNA-Seq Expression	Lsi03G021600
Synteny	Lsi03G021600

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CGCCACTCTCTCCTCTTCCTCTACAGTTTCCGAACTTATCGTCGTCGCTCTTTGAAGATAGAAGAAAAGCCCTAATTTCAGAACCTAAATTTCATCATACTTGATCTCTCTCAGACCTGTACCGTAGTTTTCAGCAGTTAAGTACCTGGTTATGCTGTAAATTCCTTCGACCATGGCGCTCCATACATCCAATTTATGCTGCAATGTGAGTTATACTTCCCTTCTTTGATTGTCTCCTACCGTTGTTATCAATTTATTTTGTTCTCATTTCTTCATTTTATCATTAATGCTTTTTTATGTGCATTAATTGCTGGACCTCAATTTTGAAATATTTCAATGATATTATGATTCTTTAGGATTATCTTACCGTTATCTGCAATCGGAAAAGCGTTCGCTGTTCGCAGAGTTTTGCGGTGTTTCATAATGTTCCTTCAGCGCTTACTAAGAAATATGAAATTGGGTTGGTTAAAGCGTCTAGAGGAGGATGTGGATTTCTTTTTCCCTCTAGTCTTCTTCATGTCGGTTCAAGGAATGGAAATTTCTGTAAGTTTCCTTGAATTTTTGTAATTTGCATGAGCCCTAATCAATTCCTCTATCTGCTGCTTTTGAAATTTGTTTGAGCTTTTATTACTTTACTTTTATGAAATCTGAACTTTCGAGCTATTGCAGTGGATATATTATGTAAAAGATTAGATGGATATACAGAGTTTTGTATAATTTCACTTTGAATTTTCTTCAGTAAAATAGCATTTTGTTAATATCTAGTGCAGGGCCCTGCAAACAAACTTCTAGGACACTCTCATTTCGACCATGTTATCTGAAAGCTGAGAATTCTAGCGGTGAAAGCATTACACTTGATGCAGAAACCTTGGAGCAGGATCTTCAAAACGCCATTGCAGATGAAGACTACGCCAGGGCTGCACAAATCAGAGATACCTTGAAAGCTCTTCAAGAGGATAGCAAAACTTCAGTATTGGCAGCCAATGCTAAGTTTTACGAATCGTTTCAGACTGGGGATCTTGCTGCAATGCAAACTCTTTGGGCACGAGGAGACAGTGTCTGCTGCGTGCACCCGGGCATGAGAGGGATTTCTGGGTATGACGATGTCGTAACGAGCTGGGAGTATGTATGGGCAAATTATGAATTCCCATTGGAGATTCAACTCAAGGACGTTCAAGTTCATGCTAGAGGAGATGTTGGATATGTTACATGTGTGGAATTGGTGAAGACAAAAGGTAGCAGTTGGGGGGGACAGTTTGTTACCAACGTGTTTGAGAGAATTAATGGTCAATGGTTCATCTGTATTCACCACGCCTCACCTATAGATTTGTAATGAGCTTTTTCCAATCATACATTTTATTCAAATGCTCAAGAAGATTGATTTCTGTCATTGCGCTTCACTCTAACACTATGGTTGCTTGAACGTCTTTTGAATTGAACAGTGAAAGGGATTTGCACCTCTA

mRNA sequence

CGCCACTCTCTCCTCTTCCTCTACAGTTTCCGAACTTATCGTCGTCGCTCTTTGAAGATAGAAGAAAAGCCCTAATTTCAGAACCTAAATTTCATCATACTTGATCTCTCTCAGACCTGTACCGTAGTTTTCAGCAGTTAAGTACCTGGTTATGCTGTAAATTCCTTCGACCATGGCGCTCCATACATCCAATTTATGCTGCAATGATTATCTTACCGTTATCTGCAATCGGAAAAGCGTTCGCTGTTCGCAGAGTTTTGCGGTGTTTCATAATGTTCCTTCAGCGCTTACTAAGAAATATGAAATTGGGTTGGTTAAAGCGTCTAGAGGAGGATGTGGATTTCTTTTTCCCTCTAGTCTTCTTCATGTCGGTTCAAGGAATGGAAATTTCTGGCCCTGCAAACAAACTTCTAGGACACTCTCATTTCGACCATGTTATCTGAAAGCTGAGAATTCTAGCGGTGAAAGCATTACACTTGATGCAGAAACCTTGGAGCAGGATCTTCAAAACGCCATTGCAGATGAAGACTACGCCAGGGCTGCACAAATCAGAGATACCTTGAAAGCTCTTCAAGAGGATAGCAAAACTTCAGTATTGGCAGCCAATGCTAAGTTTTACGAATCGTTTCAGACTGGGGATCTTGCTGCAATGCAAACTCTTTGGGCACGAGGAGACAGTGTCTGCTGCGTGCACCCGGGCATGAGAGGGATTTCTGGGTATGACGATGTCGTAACGAGCTGGGAGTATGTATGGGCAAATTATGAATTCCCATTGGAGATTCAACTCAAGGACGTTCAAGTTCATGCTAGAGGAGATGTTGGATATGTTACATGTGTGGAATTGGTGAAGACAAAAGGTAGCAGTTGGGGGGGACAGTTTGTTACCAACGTGTTTGAGAGAATTAATGGTCAATGGTTCATCTGTATTCACCACGCCTCACCTATAGATTTGTAATGAGCTTTTTCCAATCATACATTTTATTCAAATGCTCAAGAAGATTGATTTCTGTCATTGCGCTTCACTCTAACACTATGGTTGCTTGAACGTCTTTTGAATTGAACAGTGAAAGGGATTTGCACCTCTA

Coding sequence (CDS)

ATGGCGCTCCATACATCCAATTTATGCTGCAATGATTATCTTACCGTTATCTGCAATCGGAAAAGCGTTCGCTGTTCGCAGAGTTTTGCGGTGTTTCATAATGTTCCTTCAGCGCTTACTAAGAAATATGAAATTGGGTTGGTTAAAGCGTCTAGAGGAGGATGTGGATTTCTTTTTCCCTCTAGTCTTCTTCATGTCGGTTCAAGGAATGGAAATTTCTGGCCCTGCAAACAAACTTCTAGGACACTCTCATTTCGACCATGTTATCTGAAAGCTGAGAATTCTAGCGGTGAAAGCATTACACTTGATGCAGAAACCTTGGAGCAGGATCTTCAAAACGCCATTGCAGATGAAGACTACGCCAGGGCTGCACAAATCAGAGATACCTTGAAAGCTCTTCAAGAGGATAGCAAAACTTCAGTATTGGCAGCCAATGCTAAGTTTTACGAATCGTTTCAGACTGGGGATCTTGCTGCAATGCAAACTCTTTGGGCACGAGGAGACAGTGTCTGCTGCGTGCACCCGGGCATGAGAGGGATTTCTGGGTATGACGATGTCGTAACGAGCTGGGAGTATGTATGGGCAAATTATGAATTCCCATTGGAGATTCAACTCAAGGACGTTCAAGTTCATGCTAGAGGAGATGTTGGATATGTTACATGTGTGGAATTGGTGAAGACAAAAGGTAGCAGTTGGGGGGGACAGTTTGTTACCAACGTGTTTGAGAGAATTAATGGTCAATGGTTCATCTGTATTCACCACGCCTCACCTATAGATTTGTAA

Protein sequence

MALHTSNLCCNDYLTVICNRKSVRCSQSFAVFHNVPSALTKKYEIGLVKASRGGCGFLFPSSLLHVGSRNGNFWPCKQTSRTLSFRPCYLKAENSSGESITLDAETLEQDLQNAIADEDYARAAQIRDTLKALQEDSKTSVLAANAKFYESFQTGDLAAMQTLWARGDSVCCVHPGMRGISGYDDVVTSWEYVWANYEFPLEIQLKDVQVHARGDVGYVTCVELVKTKGSSWGGQFVTNVFERINGQWFICIHHASPIDL

Homology

[Close]

BLAST of Lsi03G021600 vs. ExPASy Swiss-Prot
Match: Q93YV9 (F-box protein SKIP8 OS=Arabidopsis thaliana OX=3702 GN=SKIP8 PE=1 SV=1)

HSP 1 Score: 73.9 bits (180), Expect = 2.9e-12
Identity = 37/117 (31.62%), Postives = 61/117 (52.14%), Query Frame = 0


Query: 140 SVLAANAKFYESFQTGDLAAMQTLWARGDSVCCVHPGMRGISGYDDVVTSWEYVWANYEF 199
           ++++ N +F+   +   L AM  LW   D V C+H      SGY++V+ SW+  + N+E 
Sbjct: 107 AIISVNTEFFTIIRDRALQAMARLWLNSDYVKCIHASGELFSGYNEVIQSWQLCF-NWEQ 166

Query: 200 PLEIQLKDVQVHARGDVGYVTCVELVKTKGSSWGGQFVTNVFERINGQWFICIHHAS 257
             + Q+  V+     D+ +VT    +   G   G   +TNVFE  NG+W +  HH+S
Sbjct: 167 GFDFQVHTVRTRILTDMAWVTMKAYLNVDG---GPFLITNVFEFHNGRWHMVHHHSS 219

[Close]

BLAST of Lsi03G021600 vs. ExPASy TrEMBL
Match: A0A0A0LA11 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G733200 PE=4 SV=1)

HSP 1 Score: 477.6 bits (1228), Expect = 3.2e-131
Identity = 229/260 (88.08%), Postives = 241/260 (92.69%), Query Frame = 0


Query: 1   MALHTSNLCCNDYLTVICNRKSVRCSQSFAVFHNVPSALTKKYEIGLVKASRGGCGFLFP 60
           MALHT NLCCNDY+T I +RKSVRCSQSF+VFH+VPSA  K+ EI LV +S GG GFL P
Sbjct: 1   MALHTFNLCCNDYVTFISSRKSVRCSQSFSVFHHVPSAFPKQCEIRLVNSSSGGSGFLLP 60

Query: 61  SSLLHVGSRNGNFWPCKQTSRTLSFRPCYLKAENSSGESITLDAETLEQDLQNAIADEDY 120
           S+  HVGSRNGNF PCKQ SR LSFRPC LKAENSSGESITLDAETLEQDLQNAIADEDY
Sbjct: 61  SNFRHVGSRNGNFRPCKQASRKLSFRPCQLKAENSSGESITLDAETLEQDLQNAIADEDY 120

Query: 121 ARAAQIRDTLKALQEDSKTSVLAANAKFYESFQTGDLAAMQTLWARGDSVCCVHPGMRGI 180
           ARAA+IRDTLKALQEDSKT VL ANAKFYESF+TGDLAAMQTLWARG+SVCCVHPGMRGI
Sbjct: 121 ARAAEIRDTLKALQEDSKTLVLTANAKFYESFRTGDLAAMQTLWARGNSVCCVHPGMRGI 180

Query: 181 SGYDDVVTSWEYVWANYEFPLEIQLKDVQVHARGDVGYVTCVELVKTKGSSWGGQFVTNV 240
           SGYDDV+TSWEYVWANYEFPLEIQLKD+QVHARGDVGYVTCVELVKTKGSSWGGQFVTNV
Sbjct: 181 SGYDDVITSWEYVWANYEFPLEIQLKDIQVHARGDVGYVTCVELVKTKGSSWGGQFVTNV 240

Query: 241 FERINGQWFICIHHASPIDL 261
           FERINGQWFICIHHASPIDL
Sbjct: 241 FERINGQWFICIHHASPIDL 260

[Close]

BLAST of Lsi03G021600 vs. ExPASy TrEMBL
Match: A0A5A7TRW4 (F-box protein SKIP8 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold236G004880 PE=4 SV=1)

HSP 1 Score: 464.9 bits (1195), Expect = 2.2e-127
Identity = 226/260 (86.92%), Postives = 240/260 (92.31%), Query Frame = 0


Query: 1   MALHTSNLCCNDYLTVICNRKSVRCSQSFAVFHNVPSALTKKYEIGLVKASRGGCGFLFP 60
           MAL TSNLCCNDY+T I N KSVRCSQSF+VFH+VPSAL K+ EI LVK+S GG GFL P
Sbjct: 1   MALRTSNLCCNDYVTFISNWKSVRCSQSFSVFHHVPSALPKQCEIRLVKSSNGGSGFLLP 60

Query: 61  SSLLHVGSRNGNFWPCKQTSRTLSFRPCYLKAENSSGESITLDAETLEQDLQNAIADEDY 120
           ++L HV SR+GNF PCKQ SR LSF P  LKAENSSGESITLDAETLEQDLQNAIA+EDY
Sbjct: 61  TNLRHVVSRDGNFRPCKQASRKLSFGPYQLKAENSSGESITLDAETLEQDLQNAIANEDY 120

Query: 121 ARAAQIRDTLKALQEDSKTSVLAANAKFYESFQTGDLAAMQTLWARGDSVCCVHPGMRGI 180
           ARAA+IRDTLKALQEDSKT VL ANAKFYESF+TGDLAAMQTLWARG+SVCCVHPGMRGI
Sbjct: 121 ARAAEIRDTLKALQEDSKTLVLTANAKFYESFRTGDLAAMQTLWARGNSVCCVHPGMRGI 180

Query: 181 SGYDDVVTSWEYVWANYEFPLEIQLKDVQVHARGDVGYVTCVELVKTKGSSWGGQFVTNV 240
           SGYDDV+TSWEYVWANYEFPLEIQLKD+QVHARGDVGYVTCVELVKTKGSSWGGQFVTNV
Sbjct: 181 SGYDDVITSWEYVWANYEFPLEIQLKDMQVHARGDVGYVTCVELVKTKGSSWGGQFVTNV 240

Query: 241 FERINGQWFICIHHASPIDL 261
           FERINGQWFICIHHASPIDL
Sbjct: 241 FERINGQWFICIHHASPIDL 260

[Close]

BLAST of Lsi03G021600 vs. ExPASy TrEMBL
Match: A0A1S3B5S8 (uncharacterized protein LOC103486341 OS=Cucumis melo OX=3656 GN=LOC103486341 PE=4 SV=1)

HSP 1 Score: 464.9 bits (1195), Expect = 2.2e-127
Identity = 226/260 (86.92%), Postives = 240/260 (92.31%), Query Frame = 0


Query: 1   MALHTSNLCCNDYLTVICNRKSVRCSQSFAVFHNVPSALTKKYEIGLVKASRGGCGFLFP 60
           MAL TSNLCCNDY+T I N KSVRCSQSF+VFH+VPSAL K+ EI LVK+S GG GFL P
Sbjct: 1   MALRTSNLCCNDYVTFISNWKSVRCSQSFSVFHHVPSALPKQCEIRLVKSSNGGSGFLLP 60

Query: 61  SSLLHVGSRNGNFWPCKQTSRTLSFRPCYLKAENSSGESITLDAETLEQDLQNAIADEDY 120
           ++L HV SR+GNF PCKQ SR LSF P  LKAENSSGESITLDAETLEQDLQNAIA+EDY
Sbjct: 61  TNLRHVVSRDGNFRPCKQASRKLSFGPYQLKAENSSGESITLDAETLEQDLQNAIANEDY 120

Query: 121 ARAAQIRDTLKALQEDSKTSVLAANAKFYESFQTGDLAAMQTLWARGDSVCCVHPGMRGI 180
           ARAA+IRDTLKALQEDSKT VL ANAKFYESF+TGDLAAMQTLWARG+SVCCVHPGMRGI
Sbjct: 121 ARAAEIRDTLKALQEDSKTLVLTANAKFYESFRTGDLAAMQTLWARGNSVCCVHPGMRGI 180

Query: 181 SGYDDVVTSWEYVWANYEFPLEIQLKDVQVHARGDVGYVTCVELVKTKGSSWGGQFVTNV 240
           SGYDDV+TSWEYVWANYEFPLEIQLKD+QVHARGDVGYVTCVELVKTKGSSWGGQFVTNV
Sbjct: 181 SGYDDVITSWEYVWANYEFPLEIQLKDMQVHARGDVGYVTCVELVKTKGSSWGGQFVTNV 240

Query: 241 FERINGQWFICIHHASPIDL 261
           FERINGQWFICIHHASPIDL
Sbjct: 241 FERINGQWFICIHHASPIDL 260

[Close]

BLAST of Lsi03G021600 vs. ExPASy TrEMBL
Match: A0A6J1I6J4 (uncharacterized protein LOC111470086 OS=Cucurbita maxima OX=3661 GN=LOC111470086 PE=4 SV=1)

HSP 1 Score: 459.5 bits (1181), Expect = 9.1e-126
Identity = 222/260 (85.38%), Postives = 237/260 (91.15%), Query Frame = 0


Query: 1   MALHTSNLCCNDYLTVICNRKSVRCSQSFAVFHNVPSALTKKYEIGLVKASRGGCGFLFP 60
           MALHTS+LCCN Y+T I NRK+VRCSQS  VFH+VPSAL K+Y+IG VK S GG GFLFP
Sbjct: 1   MALHTSSLCCNGYVTFISNRKNVRCSQSCEVFHHVPSALPKQYDIGFVKESTGGYGFLFP 60

Query: 61  SSLLHVGSRNGNFWPCKQTSRTLSFRPCYLKAENSSGESITLDAETLEQDLQNAIADEDY 120
           S    +GS  GNF PCKQ++R LSFRPC LKAENSSGESITLDAETL+QDLQNAIADEDY
Sbjct: 61  SIPRCLGSTIGNFRPCKQSTRMLSFRPCQLKAENSSGESITLDAETLKQDLQNAIADEDY 120

Query: 121 ARAAQIRDTLKALQEDSKTSVLAANAKFYESFQTGDLAAMQTLWARGDSVCCVHPGMRGI 180
           ARAA+IRDTLKALQEDSK SV AANAKFYESF+ GDLAAMQTLWARGDSVCCVHPGMRGI
Sbjct: 121 ARAAEIRDTLKALQEDSKASVFAANAKFYESFRNGDLAAMQTLWARGDSVCCVHPGMRGI 180

Query: 181 SGYDDVVTSWEYVWANYEFPLEIQLKDVQVHARGDVGYVTCVELVKTKGSSWGGQFVTNV 240
            GYD+V+TSWEYVWANYEFPLEIQLKDV+VHARGDVGYVTCVELVKTKGSSWGGQFVTNV
Sbjct: 181 LGYDNVMTSWEYVWANYEFPLEIQLKDVEVHARGDVGYVTCVELVKTKGSSWGGQFVTNV 240

Query: 241 FERINGQWFICIHHASPIDL 261
           FERINGQWFICIHHASPIDL
Sbjct: 241 FERINGQWFICIHHASPIDL 260

[Close]

BLAST of Lsi03G021600 vs. ExPASy TrEMBL
Match: A0A6J1FUV2 (uncharacterized protein LOC111447066 OS=Cucurbita moschata OX=3662 GN=LOC111447066 PE=4 SV=1)

HSP 1 Score: 459.1 bits (1180), Expect = 1.2e-125
Identity = 223/260 (85.77%), Postives = 238/260 (91.54%), Query Frame = 0


Query: 1   MALHTSNLCCNDYLTVICNRKSVRCSQSFAVFHNVPSALTKKYEIGLVKASRGGCGFLFP 60
           MALHTS+LCCN Y+T I NRKSVRCSQS  VF++VPSAL K+Y+IGLVKAS GG GF+ P
Sbjct: 8   MALHTSSLCCNGYVTFISNRKSVRCSQSCEVFYHVPSALPKQYDIGLVKASIGGYGFVSP 67

Query: 61  SSLLHVGSRNGNFWPCKQTSRTLSFRPCYLKAENSSGESITLDAETLEQDLQNAIADEDY 120
           S    +GS  GNF PCKQ+SR LSFRPC LKAENSSGESITLDAE L+QDLQNAIADEDY
Sbjct: 68  SIPRCLGSTIGNFRPCKQSSRMLSFRPCQLKAENSSGESITLDAEALKQDLQNAIADEDY 127

Query: 121 ARAAQIRDTLKALQEDSKTSVLAANAKFYESFQTGDLAAMQTLWARGDSVCCVHPGMRGI 180
           ARAA+IRDTLKALQEDSK SV AANAKFYESF+ GDLAAMQTLWARGDSVCCVHPGMRGI
Sbjct: 128 ARAAEIRDTLKALQEDSKASVFAANAKFYESFRNGDLAAMQTLWARGDSVCCVHPGMRGI 187

Query: 181 SGYDDVVTSWEYVWANYEFPLEIQLKDVQVHARGDVGYVTCVELVKTKGSSWGGQFVTNV 240
           SGYD+V+TSWEYVWANYEFPLEIQLKDV+VHARGDVGYVTCVELVKTKGSSWGGQFVTNV
Sbjct: 188 SGYDNVITSWEYVWANYEFPLEIQLKDVEVHARGDVGYVTCVELVKTKGSSWGGQFVTNV 247

Query: 241 FERINGQWFICIHHASPIDL 261
           FERINGQWFICIHHASPIDL
Sbjct: 248 FERINGQWFICIHHASPIDL 267

[Close]

BLAST of Lsi03G021600 vs. NCBI nr
Match: XP_038904182.1 (uncharacterized protein LOC120090530 [Benincasa hispida] >XP_038904693.1 uncharacterized protein LOC120091000 [Benincasa hispida])

HSP 1 Score: 495.0 bits (1273), Expect = 4.0e-136
Identity = 237/260 (91.15%), Postives = 249/260 (95.77%), Query Frame = 0


Query: 1   MALHTSNLCCNDYLTVICNRKSVRCSQSFAVFHNVPSALTKKYEIGLVKASRGGCGFLFP 60
           MALHTSNLCCND++T ICNRKSVRC Q+FAVFH++PSAL K+ EIGLVK+ RGGCGFLFP
Sbjct: 1   MALHTSNLCCNDHVTFICNRKSVRCLQNFAVFHHLPSALPKQCEIGLVKSCRGGCGFLFP 60

Query: 61  SSLLHVGSRNGNFWPCKQTSRTLSFRPCYLKAENSSGESITLDAETLEQDLQNAIADEDY 120
           SSL +V SRNGNF P KQTSRTLSFRPC LKAENSSGESITLDAETLEQDLQNAIADEDY
Sbjct: 61  SSLRYVASRNGNFRPWKQTSRTLSFRPCQLKAENSSGESITLDAETLEQDLQNAIADEDY 120

Query: 121 ARAAQIRDTLKALQEDSKTSVLAANAKFYESFQTGDLAAMQTLWARGDSVCCVHPGMRGI 180
           ARAAQIRDTLKALQEDSKTSVLAANAKFY+SFQTGDLA MQTLWARGDSVCCVHPGMRGI
Sbjct: 121 ARAAQIRDTLKALQEDSKTSVLAANAKFYKSFQTGDLATMQTLWARGDSVCCVHPGMRGI 180

Query: 181 SGYDDVVTSWEYVWANYEFPLEIQLKDVQVHARGDVGYVTCVELVKTKGSSWGGQFVTNV 240
           SGY+DV+TSWEYVWANYEFPLEIQLKDVQVHARGDVGYVTCVELVKTKGSSWGGQFVTNV
Sbjct: 181 SGYNDVITSWEYVWANYEFPLEIQLKDVQVHARGDVGYVTCVELVKTKGSSWGGQFVTNV 240

Query: 241 FERINGQWFICIHHASPIDL 261
           FERINGQWF+CIHHASPIDL
Sbjct: 241 FERINGQWFVCIHHASPIDL 260

[Close]

BLAST of Lsi03G021600 vs. NCBI nr
Match: XP_004137747.1 (uncharacterized protein LOC101219393 [Cucumis sativus] >KGN58815.1 hypothetical protein Csa_001436 [Cucumis sativus])

HSP 1 Score: 477.6 bits (1228), Expect = 6.7e-131
Identity = 229/260 (88.08%), Postives = 241/260 (92.69%), Query Frame = 0


Query: 1   MALHTSNLCCNDYLTVICNRKSVRCSQSFAVFHNVPSALTKKYEIGLVKASRGGCGFLFP 60
           MALHT NLCCNDY+T I +RKSVRCSQSF+VFH+VPSA  K+ EI LV +S GG GFL P
Sbjct: 1   MALHTFNLCCNDYVTFISSRKSVRCSQSFSVFHHVPSAFPKQCEIRLVNSSSGGSGFLLP 60

Query: 61  SSLLHVGSRNGNFWPCKQTSRTLSFRPCYLKAENSSGESITLDAETLEQDLQNAIADEDY 120
           S+  HVGSRNGNF PCKQ SR LSFRPC LKAENSSGESITLDAETLEQDLQNAIADEDY
Sbjct: 61  SNFRHVGSRNGNFRPCKQASRKLSFRPCQLKAENSSGESITLDAETLEQDLQNAIADEDY 120

Query: 121 ARAAQIRDTLKALQEDSKTSVLAANAKFYESFQTGDLAAMQTLWARGDSVCCVHPGMRGI 180
           ARAA+IRDTLKALQEDSKT VL ANAKFYESF+TGDLAAMQTLWARG+SVCCVHPGMRGI
Sbjct: 121 ARAAEIRDTLKALQEDSKTLVLTANAKFYESFRTGDLAAMQTLWARGNSVCCVHPGMRGI 180

Query: 181 SGYDDVVTSWEYVWANYEFPLEIQLKDVQVHARGDVGYVTCVELVKTKGSSWGGQFVTNV 240
           SGYDDV+TSWEYVWANYEFPLEIQLKD+QVHARGDVGYVTCVELVKTKGSSWGGQFVTNV
Sbjct: 181 SGYDDVITSWEYVWANYEFPLEIQLKDIQVHARGDVGYVTCVELVKTKGSSWGGQFVTNV 240

Query: 241 FERINGQWFICIHHASPIDL 261
           FERINGQWFICIHHASPIDL
Sbjct: 241 FERINGQWFICIHHASPIDL 260

[Close]

BLAST of Lsi03G021600 vs. NCBI nr
Match: XP_023539336.1 (uncharacterized protein LOC111799999 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 466.5 bits (1199), Expect = 1.5e-127
Identity = 225/260 (86.54%), Postives = 239/260 (91.92%), Query Frame = 0


Query: 1   MALHTSNLCCNDYLTVICNRKSVRCSQSFAVFHNVPSALTKKYEIGLVKASRGGCGFLFP 60
           MALHTS+LCCN Y+T I NRKSVRCSQS  VFH+VPSA  K+Y+IGLVKAS GGCGFLFP
Sbjct: 8   MALHTSSLCCNGYVTFISNRKSVRCSQSCEVFHHVPSARPKQYDIGLVKASTGGCGFLFP 67

Query: 61  SSLLHVGSRNGNFWPCKQTSRTLSFRPCYLKAENSSGESITLDAETLEQDLQNAIADEDY 120
           S    +GS  GNF PCK ++R LSFRPC LKAENSSGESITLDAETL+QDLQNAIADEDY
Sbjct: 68  SIPRCLGSTIGNFRPCKLSTRMLSFRPCQLKAENSSGESITLDAETLKQDLQNAIADEDY 127

Query: 121 ARAAQIRDTLKALQEDSKTSVLAANAKFYESFQTGDLAAMQTLWARGDSVCCVHPGMRGI 180
           ARAA+IRDTLKALQEDSK SV AANAKFYESF+ GDLAAMQTLWARGDSVCCVHPGMRGI
Sbjct: 128 ARAAEIRDTLKALQEDSKASVFAANAKFYESFRNGDLAAMQTLWARGDSVCCVHPGMRGI 187

Query: 181 SGYDDVVTSWEYVWANYEFPLEIQLKDVQVHARGDVGYVTCVELVKTKGSSWGGQFVTNV 240
           SGYD+V+TSWEYVWANYEFPLEIQLKDV+VHARGDVGYVTCVELVKTKGSSWGGQFVTNV
Sbjct: 188 SGYDNVITSWEYVWANYEFPLEIQLKDVEVHARGDVGYVTCVELVKTKGSSWGGQFVTNV 247

Query: 241 FERINGQWFICIHHASPIDL 261
           FERINGQWFICIHHASPIDL
Sbjct: 248 FERINGQWFICIHHASPIDL 267

[Close]

BLAST of Lsi03G021600 vs. NCBI nr
Match: XP_008442487.1 (PREDICTED: uncharacterized protein LOC103486341 [Cucumis melo] >KAA0044135.1 F-box protein SKIP8 [Cucumis melo var. makuwa])

HSP 1 Score: 464.9 bits (1195), Expect = 4.5e-127
Identity = 226/260 (86.92%), Postives = 240/260 (92.31%), Query Frame = 0


Query: 1   MALHTSNLCCNDYLTVICNRKSVRCSQSFAVFHNVPSALTKKYEIGLVKASRGGCGFLFP 60
           MAL TSNLCCNDY+T I N KSVRCSQSF+VFH+VPSAL K+ EI LVK+S GG GFL P
Sbjct: 1   MALRTSNLCCNDYVTFISNWKSVRCSQSFSVFHHVPSALPKQCEIRLVKSSNGGSGFLLP 60

Query: 61  SSLLHVGSRNGNFWPCKQTSRTLSFRPCYLKAENSSGESITLDAETLEQDLQNAIADEDY 120
           ++L HV SR+GNF PCKQ SR LSF P  LKAENSSGESITLDAETLEQDLQNAIA+EDY
Sbjct: 61  TNLRHVVSRDGNFRPCKQASRKLSFGPYQLKAENSSGESITLDAETLEQDLQNAIANEDY 120

Query: 121 ARAAQIRDTLKALQEDSKTSVLAANAKFYESFQTGDLAAMQTLWARGDSVCCVHPGMRGI 180
           ARAA+IRDTLKALQEDSKT VL ANAKFYESF+TGDLAAMQTLWARG+SVCCVHPGMRGI
Sbjct: 121 ARAAEIRDTLKALQEDSKTLVLTANAKFYESFRTGDLAAMQTLWARGNSVCCVHPGMRGI 180

Query: 181 SGYDDVVTSWEYVWANYEFPLEIQLKDVQVHARGDVGYVTCVELVKTKGSSWGGQFVTNV 240
           SGYDDV+TSWEYVWANYEFPLEIQLKD+QVHARGDVGYVTCVELVKTKGSSWGGQFVTNV
Sbjct: 181 SGYDDVITSWEYVWANYEFPLEIQLKDMQVHARGDVGYVTCVELVKTKGSSWGGQFVTNV 240

Query: 241 FERINGQWFICIHHASPIDL 261
           FERINGQWFICIHHASPIDL
Sbjct: 241 FERINGQWFICIHHASPIDL 260

[Close]

BLAST of Lsi03G021600 vs. NCBI nr
Match: KAG6596375.1 (F-box protein SKIP8, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 459.9 bits (1182), Expect = 1.4e-125
Identity = 223/260 (85.77%), Postives = 238/260 (91.54%), Query Frame = 0


Query: 1   MALHTSNLCCNDYLTVICNRKSVRCSQSFAVFHNVPSALTKKYEIGLVKASRGGCGFLFP 60
           MALHTS+LCCN Y+T I NRKSVRCSQS  VFH+VP AL K+Y+IGLVKAS GG GF+ P
Sbjct: 8   MALHTSSLCCNGYVTFISNRKSVRCSQSCEVFHHVPLALPKQYDIGLVKASIGGYGFVSP 67

Query: 61  SSLLHVGSRNGNFWPCKQTSRTLSFRPCYLKAENSSGESITLDAETLEQDLQNAIADEDY 120
           S    +GS  GNF PCKQ++R LSFRPC LKAENSSGESITLDAETL+QDLQNAIADEDY
Sbjct: 68  SIPRCLGSTIGNFRPCKQSTRMLSFRPCQLKAENSSGESITLDAETLKQDLQNAIADEDY 127

Query: 121 ARAAQIRDTLKALQEDSKTSVLAANAKFYESFQTGDLAAMQTLWARGDSVCCVHPGMRGI 180
           ARAA+IRDTLKALQEDSK SV AANAKFYESF+ GDLAAMQTLWARGDSVCCVHPGMRGI
Sbjct: 128 ARAAEIRDTLKALQEDSKASVFAANAKFYESFRNGDLAAMQTLWARGDSVCCVHPGMRGI 187

Query: 181 SGYDDVVTSWEYVWANYEFPLEIQLKDVQVHARGDVGYVTCVELVKTKGSSWGGQFVTNV 240
           SGYD+V+TSWEYVWANYEFPLEIQLKDV+VHARGDVGYVTCVELVKTKGSSWGGQFVTNV
Sbjct: 188 SGYDNVITSWEYVWANYEFPLEIQLKDVEVHARGDVGYVTCVELVKTKGSSWGGQFVTNV 247

Query: 241 FERINGQWFICIHHASPIDL 261
           FERINGQWFICIHHASPIDL
Sbjct: 248 FERINGQWFICIHHASPIDL 267

[Close]

BLAST of Lsi03G021600 vs. TAIR 10
Match: AT3G09250.1 (Nuclear transport factor 2 (NTF2) family protein )

HSP 1 Score: 255.4 bits (651), Expect = 5.0e-68
Identity = 117/180 (65.00%), Postives = 149/180 (82.78%), Query Frame = 0


Query: 86  RPCYLKAENSSG----ESITLDAETLEQDLQNAIADEDYARAAQIRDTLKALQEDSKTSV 145
           RPC +K E ++     ESI++D  TL+QDL+ A+ +E+Y  AA+IRD LK LQED+K SV
Sbjct: 65  RPCRVKREENNQTADVESISMDENTLKQDLETAVQEENYVEAAKIRDKLKELQEDNKASV 124

Query: 146 LAANAKFYESFQTGDLAAMQTLWARGDSVCCVHPGMRGISGYDDVVTSWEYVWANYEFPL 205
           L+AN++FY+SF+ GDLAAMQ+LW++  + CCVHPG +GI+GYD V+ SWE VW NYEFPL
Sbjct: 125 LSANSRFYQSFRNGDLAAMQSLWSKSGNPCCVHPGAKGITGYDYVMESWELVWMNYEFPL 184

Query: 206 EIQLKDVQVHARGDVGYVTCVELVKTKG-SSWGGQFVTNVFERINGQWFICIHHASPIDL 261
            I+LKDV+VH RG+VGYVTC+E VKTKG SSWG QFV+NVFERI+GQWFICIHHASP+D+
Sbjct: 185 LIELKDVEVHVRGEVGYVTCMEFVKTKGSSSWGAQFVSNVFERIDGQWFICIHHASPVDI 244

[Close]

BLAST of Lsi03G021600 vs. TAIR 10
Match: AT3G09250.2 (Nuclear transport factor 2 (NTF2) family protein )

HSP 1 Score: 229.2 bits (583), Expect = 3.9e-60
Identity = 107/168 (63.69%), Postives = 137/168 (81.55%), Query Frame = 0


Query: 86  RPCYLKAENSSG----ESITLDAETLEQDLQNAIADEDYARAAQIRDTLKALQEDSKTSV 145
           RPC +K E ++     ESI++D  TL+QDL+ A+ +E+Y  AA+IRD LK LQED+K SV
Sbjct: 51  RPCRVKREENNQTADVESISMDENTLKQDLETAVQEENYVEAAKIRDKLKELQEDNKASV 110

Query: 146 LAANAKFYESFQTGDLAAMQTLWARGDSVCCVHPGMRGISGYDDVVTSWEYVWANYEFPL 205
           L+AN++FY+SF+ GDLAAMQ+LW++  + CCVHPG +GI+GYD V+ SWE VW NYEFPL
Sbjct: 111 LSANSRFYQSFRNGDLAAMQSLWSKSGNPCCVHPGAKGITGYDYVMESWELVWMNYEFPL 170

Query: 206 EIQLKDVQVHARGDVGYVTCVELVKTKG-SSWGGQFVTNVFERINGQW 249
            I+LKDV+VH RG+VGYVTC+E VKTKG SSWG QFV+NVFERI+GQW
Sbjct: 171 LIELKDVEVHVRGEVGYVTCMEFVKTKGSSSWGAQFVSNVFERIDGQW 218

[Close]

BLAST of Lsi03G021600 vs. TAIR 10
Match: AT4G10925.1 (Nuclear transport factor 2 (NTF2) family protein )

HSP 1 Score: 73.9 bits (180), Expect = 2.1e-13
Identity = 37/117 (31.62%), Postives = 61/117 (52.14%), Query Frame = 0


Query: 140 SVLAANAKFYESFQTGDLAAMQTLWARGDSVCCVHPGMRGISGYDDVVTSWEYVWANYEF 199
           ++++ N +F+   +   L AM  LW   D V C+H      SGY++V+ SW+  + N+E 
Sbjct: 107 AIISVNTEFFTIIRDRALQAMARLWLNSDYVKCIHASGELFSGYNEVIQSWQLCF-NWEQ 166

Query: 200 PLEIQLKDVQVHARGDVGYVTCVELVKTKGSSWGGQFVTNVFERINGQWFICIHHAS 257
             + Q+  V+     D+ +VT    +   G   G   +TNVFE  NG+W +  HH+S
Sbjct: 167 GFDFQVHTVRTRILTDMAWVTMKAYLNVDG---GPFLITNVFEFHNGRWHMVHHHSS 219

[Close]

BLAST of Lsi03G021600 vs. TAIR 10
Match: AT4G10925.2 (Nuclear transport factor 2 (NTF2) family protein )

HSP 1 Score: 73.9 bits (180), Expect = 2.1e-13
Identity = 37/117 (31.62%), Postives = 61/117 (52.14%), Query Frame = 0


Query: 140 SVLAANAKFYESFQTGDLAAMQTLWARGDSVCCVHPGMRGISGYDDVVTSWEYVWANYEF 199
           ++++ N +F+   +   L AM  LW   D V C+H      SGY++V+ SW+  + N+E 
Sbjct: 107 AIISVNTEFFTIIRDRALQAMARLWLNSDYVKCIHASGELFSGYNEVIQSWQLCF-NWEQ 166

Query: 200 PLEIQLKDVQVHARGDVGYVTCVELVKTKGSSWGGQFVTNVFERINGQWFICIHHAS 257
             + Q+  V+     D+ +VT    +   G   G   +TNVFE  NG+W +  HH+S
Sbjct: 167 GFDFQVHTVRTRILTDMAWVTMKAYLNVDG---GPFLITNVFEFHNGRWHMVHHHSS 219

[Close]

BLAST of Lsi03G021600 vs. TAIR 10
Match: AT4G10925.3 (Nuclear transport factor 2 (NTF2) family protein )

HSP 1 Score: 73.9 bits (180), Expect = 2.1e-13
Identity = 37/117 (31.62%), Postives = 61/117 (52.14%), Query Frame = 0


Query: 140 SVLAANAKFYESFQTGDLAAMQTLWARGDSVCCVHPGMRGISGYDDVVTSWEYVWANYEF 199
           ++++ N +F+   +   L AM  LW   D V C+H      SGY++V+ SW+  + N+E 
Sbjct: 107 AIISVNTEFFTIIRDRALQAMARLWLNSDYVKCIHASGELFSGYNEVIQSWQLCF-NWEQ 166

Query: 200 PLEIQLKDVQVHARGDVGYVTCVELVKTKGSSWGGQFVTNVFERINGQWFICIHHAS 257
             + Q+  V+     D+ +VT    +   G   G   +TNVFE  NG+W +  HH+S
Sbjct: 167 GFDFQVHTVRTRILTDMAWVTMKAYLNVDG---GPFLITNVFEFHNGRWHMVHHHSS 219

The following BLAST results are available for this feature:

BLAST of Lsi03G021600 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q93YV9	2.9e-12	31.62	F-box protein SKIP8 OS=Arabidopsis thaliana OX=3702 GN=SKIP8 PE=1 SV=1	[more]

BLAST of Lsi03G021600 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LA11	3.2e-131	88.08	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G733200 PE=4 SV=1	[more]
A0A5A7TRW4	2.2e-127	86.92	F-box protein SKIP8 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold236G...	[more]
A0A1S3B5S8	2.2e-127	86.92	uncharacterized protein LOC103486341 OS=Cucumis melo OX=3656 GN=LOC103486341 PE=...	[more]
A0A6J1I6J4	9.1e-126	85.38	uncharacterized protein LOC111470086 OS=Cucurbita maxima OX=3661 GN=LOC111470086...	[more]
A0A6J1FUV2	1.2e-125	85.77	uncharacterized protein LOC111447066 OS=Cucurbita moschata OX=3662 GN=LOC1114470...	[more]

BLAST of Lsi03G021600 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038904182.1	4.0e-136	91.15	uncharacterized protein LOC120090530 [Benincasa hispida] >XP_038904693.1 unchara...	[more]
XP_004137747.1	6.7e-131	88.08	uncharacterized protein LOC101219393 [Cucumis sativus] >KGN58815.1 hypothetical ...	[more]
XP_023539336.1	1.5e-127	86.54	uncharacterized protein LOC111799999 [Cucurbita pepo subsp. pepo]	[more]
XP_008442487.1	4.5e-127	86.92	PREDICTED: uncharacterized protein LOC103486341 [Cucumis melo] >KAA0044135.1 F-b...	[more]
KAG6596375.1	1.4e-125	85.77	F-box protein SKIP8, partial [Cucurbita argyrosperma subsp. sororia]	[more]

BLAST of Lsi03G021600 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G09250.1	5.0e-68	65.00	Nuclear transport factor 2 (NTF2) family protein	[more]
AT3G09250.2	3.9e-60	63.69	Nuclear transport factor 2 (NTF2) family protein	[more]
AT4G10925.1	2.1e-13	31.62	Nuclear transport factor 2 (NTF2) family protein	[more]
AT4G10925.2	2.1e-13	31.62	Nuclear transport factor 2 (NTF2) family protein	[more]
AT4G10925.3	2.1e-13	31.62	Nuclear transport factor 2 (NTF2) family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18

Position :

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x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001943	UVR domain	PFAM	PF02151	UVR	coord: 105..135 e-value: 1.1E-6 score: 28.1
IPR001943	UVR domain	PROSITE	PS50151	UVR	coord: 101..136 score: 8.313559
None	No IPR available	GENE3D	3.10.450.50		coord: 130..259 e-value: 3.3E-42 score: 145.9
None	No IPR available	PANTHER	PTHR34957	NUCLEAR TRANSPORT FACTOR 2 (NTF2) FAMILY PROTEIN	coord: 21..259
IPR037401	SnoaL-like domain	PFAM	PF13474	SnoaL_3	coord: 141..257 e-value: 1.6E-23 score: 83.4
IPR032710	NTF2-like domain superfamily	SUPERFAMILY	54427	NTF2-like	coord: 136..258

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi03G021600.1	Lsi03G021600.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0005515	protein binding