Lsi03G021540 (gene) Bottle gourd (USVL1VR-Ls) v1

BLAST of Lsi03G021540 vs. ExPASy Swiss-Prot
Match: Q9LZN0 (Rop guanine nucleotide exchange factor 7 OS=Arabidopsis thaliana OX=3702 GN=ROPGEF7 PE=1 SV=1)

HSP 1 Score: 551.6 bits (1420), Expect = 1.3e-155
Identity = 299/479 (62.42%), Postives = 362/479 (75.57%), Query Frame = 0

Query: 211 GNSQKMFDEKTGSTSSSDLLTSEETGELEEQSLRDEESSSSSEASMGWPLHKTAGEGEEG 270
           G+S+ + + +     SS   +     E EEQS    E  ++S  S  W +    GE    
Sbjct: 3   GSSENLPEVEEKGRESSCCSSETTRQEEEEQSPSCTEDFTASPVSSRWSVKNIDGE---- 62

Query: 271 VEKEVLTD---SETEMMKERFSKLLLGEDMSGCGNGVCTALAISNAITNLC--------- 330
            +K++ +D   SE EMMKERFSKLLLGEDMSG GNGVCTALAISNAITNLC         
Sbjct: 63  -KKKIRSDSRVSEVEMMKERFSKLLLGEDMSGSGNGVCTALAISNAITNLCATLFGQLWR 122

Query: 331 ----EAERKAMWGREMELLLCVSNHIVELIPIWQTFPDGTKLEIMTCRPRSDLYVNLPAL 390
                 E+K MW REME LLCVS+HIVE+ P WQTFPDGTKLEIMTCRPRSDLYVNLPAL
Sbjct: 123 LEPLPTEKKEMWRREMEWLLCVSDHIVEMTPTWQTFPDGTKLEIMTCRPRSDLYVNLPAL 182

Query: 391 RKLDHMLLDILDSFVDSEFWYVDQGILAADQ-ADSSSSFRKVLERQDEKWWLPIPRVPNG 450
           RKLD+MLL+ILDSF ++EFWYVDQGI+A +  AD SSSFRK  +RQ++KWWLP+PRV  G
Sbjct: 183 RKLDNMLLEILDSFEETEFWYVDQGIMAHESAADGSSSFRKSFQRQEDKWWLPVPRVSPG 242

Query: 451 GLSESSTRQLHHKRDCTNQILKAAVAINSLTLADMDVPISYLESLPKNGRASLGEAIYKY 510
           GL E+S +QL HKRDCTNQILKAA+AINS+TLADM++P SYLESLP+ GR+ LG+ IY+Y
Sbjct: 243 GLQENSRKQLQHKRDCTNQILKAAMAINSITLADMEIPESYLESLPRKGRSCLGDLIYRY 302

Query: 511 ISSDEFSPVLLLECLDISSEHQAIEIANRVEAAMYAWRK---GIATNNSKSSWEMLKELV 570
           ISSD+FSP  LL+CLD+SSEHQAIEIANRVE+++Y W K        N+K+SWEM+KEL+
Sbjct: 303 ISSDQFSPECLLDCLDLSSEHQAIEIANRVESSIYLWHKRTNSKPATNTKTSWEMVKELM 362

Query: 571 IDADKSEVLAERAELVLLCLKQQFPNLPQTSLDMSKIQYNKDVGKAILESYSRVLESLAY 630
           +DADK E++A+RAE +LL LKQ+FP LPQT+LDMSKIQYNKD+GK+ILESYSRVLESLA+
Sbjct: 363 VDADKLELMADRAESLLLSLKQRFPGLPQTALDMSKIQYNKDIGKSILESYSRVLESLAF 422

Query: 631 NIVARIEDLLYVNDLTKH-SDQIPGISKLGIVAHNSSNRIQISVPFSSSPYTTKFIKPS 669
           NIVARI+DLL+V+DLT+H SDQIP  + LG    N+ N    S+    S YTT    PS
Sbjct: 423 NIVARIDDLLFVDDLTRHSSDQIP--TTLG----NNGNDAPKSIAVPVSNYTTPSYSPS 470

BLAST of Lsi03G021540 vs. ExPASy Swiss-Prot
Match: F4K295 (Rop guanine nucleotide exchange factor 5 OS=Arabidopsis thaliana OX=3702 GN=ROPGEF5 PE=2 SV=1)

HSP 1 Score: 482.3 bits (1240), Expect = 9.6e-135
Identity = 270/523 (51.63%), Postives = 355/523 (67.88%), Query Frame = 0

Query: 177 CRGLEYRGMVVNNSAFLEVVAMEIEGKEEKCDVFGNSQKMFDEKTGSTSSSDLLTSEETG 236
           C G+E +    N +  +E +  E    +E+    G S +     T ++SS     S+  G
Sbjct: 8   CAGIEKKRS--NLTPSMEDILTE---SKERSSTSGASYESSSTTTVASSSPPPPPSQILG 67

Query: 237 ELEEQSLRDEESSSSSEASMGWPLHKTAGEGEEGVEKEVLTDSETEMMKERFSKLLLGED 296
                 +R      +S+ S+ +   K     + G + + +  ++ EMMKERF+KLLLGED
Sbjct: 68  ----WPIRKASFRKNSKESVNFDHKKLTLHDDSGFKAKEMNSADVEMMKERFAKLLLGED 127

Query: 297 MSGCGNGVCTALAISNAITNLC-------------EAERKAMWGREMELLLCVSNHIVEL 356
           MSG G GVCTALAISNAITNLC              +E+K MW REME +L VS+HIVEL
Sbjct: 128 MSGSGKGVCTALAISNAITNLCATIFGQLWRLEPLSSEKKEMWRREMEWILSVSDHIVEL 187

Query: 357 IPIWQTFPDGTKLEIMTCRPRSDLYVNLPALRKLDHMLLDILDSFVDSEFWYVDQGILAA 416
            P  QT+PDG K E+MTCRPR DL++NLPALRKLD+MLLDIL SF  +EFWYVDQGI+A+
Sbjct: 188 TPSTQTYPDGNKFEVMTCRPRFDLFINLPALRKLDNMLLDILASFKKTEFWYVDQGIVAS 247

Query: 417 DQADSSSSFRKVLERQDEKWWLPIPRVPNGGLSESSTRQLHHKRDCTNQILKAAVAINSL 476
           +  D S+SFR+ ++RQ+EKWWLP+PR+   GL+E +  +L+HKR+C  QILKAA+AINSL
Sbjct: 248 EN-DGSASFRRKIQRQEEKWWLPVPRLAPNGLTEEARTELNHKRECATQILKAAMAINSL 307

Query: 477 TLADMDVPISYLESLPKNGRASLGEAIYKYISSDEFSPVLLLECLDISSEHQAIEIANRV 536
            L +MDVP SYLE+LPKNGR+ LG+ IY+Y++SD+FS   LL+CLD+SSEH A++IANRV
Sbjct: 308 ALTEMDVPKSYLETLPKNGRSCLGDVIYRYVTSDKFSAESLLDCLDLSSEHIALDIANRV 367

Query: 537 EAAMYAWRKGIAT----NNSKSS------WEMLKELVIDADKSEVLAERAELVLLCLKQQ 596
           EA++Y WR+ + T    NN+ SS      WEM+KEL+   DK  +L ER+E +L CLKQ+
Sbjct: 368 EASIYVWRRRVQTKLGVNNNTSSTTPKLTWEMVKELMAAGDKRGLLVERSETLLRCLKQR 427

Query: 597 FPNLPQTSLDMSKIQYNKDVGKAILESYSRVLESLAYNIVARIEDLLYVNDLTKHSD--- 656
           FP+L QTSLD+SKIQ+NKD+GK+ILESYSR LESLA NI+ARI+DLLYV+DLTK SD   
Sbjct: 428 FPSLTQTSLDISKIQWNKDIGKSILESYSRALESLASNIIARIDDLLYVDDLTKQSDDNN 487

Query: 657 --QIPGISKLGIVAHNSSNRIQISVPFSSSPYTTKF-IKPSFS 671
               P +S   I+AH     +   +  S +PY T F   P FS
Sbjct: 488 LLSSPAVS--SIIAHKKVVPLPYLISASGTPYRTSFSTTPGFS 518

BLAST of Lsi03G021540 vs. ExPASy Swiss-Prot
Match: Q9M056 (Rop guanine nucleotide exchange factor 6 OS=Arabidopsis thaliana OX=3702 GN=ROPGEF6 PE=2 SV=1)

HSP 1 Score: 420.6 bits (1080), Expect = 3.4e-116
Identity = 258/538 (47.96%), Postives = 351/538 (65.24%), Query Frame = 0

Query: 210 FGNSQK----MFDEKTGSTSSSDLLTSEE-TGELEEQSLRDEESSSSSEAS---MGWPL- 269
           FG + K    + D  T ST+ S L +S    G    +S   E S SS       +GWPL 
Sbjct: 15  FGETSKRIIGLIDSVTESTTDSSLSSSSSGVGSSSGRSSVAERSVSSPPTKSQILGWPLG 74

Query: 270 ----HKTAGEGEE---------GVEKEVLTDSETEMMKERFSKLLLGEDMSGCGNGVCTA 329
                K++G+ ++         G ++     SE E++KER +KLLLGEDMSG G GVC A
Sbjct: 75  QGSWRKSSGKMKKKTPTKIDDFGFKRVGTETSEIELLKERMAKLLLGEDMSGSGEGVCPA 134

Query: 330 LAISNAITNLCEA-------------ERKAMWGREMELLLCVSNHIVELIPIWQTFPDGT 389
           LAISNAITNL  A             E+K MW RE+E+LL VS+HIVEL+P +Q FP+G 
Sbjct: 135 LAISNAITNLYAAILGQQWRLEPIPSEKKLMWRREIEVLLSVSDHIVELVPSFQNFPNGN 194

Query: 390 KLEIMTCRPRSDLYVNLPALRKLDHMLLDILDSFVDSEFWYVDQGILAADQADSSSSFRK 449
           K+E+M CRPRSDL+  LPALRKLD+ML++ILDSF ++EFWYVDQGI+AA+ A  S+SFR+
Sbjct: 195 KIEVMNCRPRSDLFTCLPALRKLDNMLIEILDSFGETEFWYVDQGIVAAESA-RSNSFRE 254

Query: 450 VLERQDEKWWLPIPRVPNGGLSESSTRQLHHKRDCTNQILKAAVAINSLTLADMDVPISY 509
                 +KWWLP+PRVP+ GL+E + ++L H R+ TNQILKA ++INS+ LA+M+VP SY
Sbjct: 255 ----DGDKWWLPLPRVPSDGLTEQTRKKLDHTREFTNQILKACMSINSIALAEMEVPQSY 314

Query: 510 LESLPKNGRASLGEAIYKYISSDEFSPVLLLECLDISSEHQAIEIANRVEAAMYAWRKGI 569
           LE+LPKNGR+ LG+ +Y+ I+SD FS   LLE +D+SSE   +E+ANRVEA+MY WR+  
Sbjct: 315 LEALPKNGRSCLGDFLYRNITSDNFSADHLLESIDLSSELAVVEMANRVEASMYVWRRRA 374

Query: 570 AT-------NNSKSSWEML-KELVI---DADKSEVLAERAELVLLCLKQQFPNLPQTSLD 629
            +        ++ + W M+ KE+++   D DK E+ AERAE +L+ LKQ+FP L QT+LD
Sbjct: 375 HSRHLISLYRSTSTRWGMIVKEMMMHQTDGDKREIFAERAESLLIRLKQRFPGLRQTALD 434

Query: 630 MSKIQYNKDVGKAILESYSRVLESLAYNIVARIEDLLYVNDLTKH---------SDQIPG 682
            SKIQYNKDVGK+ILESYSRVLESLAY+I  RIE++L+++D++K          SD++  
Sbjct: 435 TSKIQYNKDVGKSILESYSRVLESLAYSIGVRIEEVLFMDDISKDDGDGDDDSCSDKLRL 494

BLAST of Lsi03G021540 vs. ExPASy Swiss-Prot
Match: Q93ZY2 (Rop guanine nucleotide exchange factor 1 OS=Arabidopsis thaliana OX=3702 GN=ROPGEF1 PE=1 SV=2)

HSP 1 Score: 410.2 bits (1053), Expect = 4.6e-113
Identity = 246/496 (49.60%), Postives = 322/496 (64.92%), Query Frame = 0

Query: 219 EKTGSTS-SSDLLTSEETGELEEQSLRDEESSSSSEAS----------MGWPLHKTAGEG 278
           E+ GS S S+D+  SE +          E +SSS  +S             P+      G
Sbjct: 15  ERCGSYSPSADISESESSSSFSCHRFDGEGASSSIPSSPRVVAGRGFYFPAPVMLPVIGG 74

Query: 279 EEGVEKEVLTD---SETEMMKERFSKLLLGEDMSGCGNGVCTALAISNAITNLCEA---- 338
           ++ V  +   D   SE EMMKERF+KLLLGEDMSG G GVCTALAISNAITNL       
Sbjct: 75  KDVVWDDKQPDNDLSEIEMMKERFAKLLLGEDMSGGGKGVCTALAISNAITNLSATVFGE 134

Query: 339 ---------ERKAMWGREMELLLCVSNHIVELIPIWQTFPDGTKLEIMTCRPRSDLYVNL 398
                    ++KAMW RE+E LLCVS+ IVELIP  Q FP G   EIM  RPRSDLY NL
Sbjct: 135 LWRLEPLAPQKKAMWRRELEWLLCVSDSIVELIPSIQQFPGGGTYEIMETRPRSDLYANL 194

Query: 399 PALRKLDHMLLDILDSFVDSEFWYVDQGILAADQADSSSSFRKVLERQDEKWWLPIPRVP 458
           PAL+KLD ML+D+LD+F D+EFWY D+GI+  D  D  S       RQ++KWWLP P+VP
Sbjct: 195 PALKKLDAMLIDMLDAFSDTEFWYTDRGIVLGD-CDKDSYNSPASVRQEDKWWLPCPKVP 254

Query: 459 NGGLSESSTRQLHHKRDCTNQILKAAVAINSLTLADMDVPISYLESLPKNGRASLGEAIY 518
             GLSE + ++L   RD  NQILKAA+AINS  LA+M++P  YLE+LPK+G+  LGE IY
Sbjct: 255 PNGLSEEARKKLQQCRDFANQILKAALAINSGVLAEMEIPDPYLETLPKSGKECLGEIIY 314

Query: 519 KYISSDEFSPVLLLECLDISSEHQAIEIANRVEAAMYAWRKGIATNNSK------SSW-E 578
           +Y+++++FSP  LL+CLD+SSEHQ +EIANR+EAA++ WR+     + K      SSW  
Sbjct: 315 QYLTANKFSPECLLDCLDLSSEHQTLEIANRIEAAVHVWRQKNGRRHKKQAKLKLSSWGG 374

Query: 579 MLKELVIDADKSEVLAERAELVLLCLKQQFPNLPQTSLDMSKIQYNKDVGKAILESYSRV 638
            +K LV D ++++ L +RAE +L  L+ +FP LPQT+LDM+KIQYNKDVG++ILESYSRV
Sbjct: 375 KVKGLVNDNERNDFLVQRAETLLQSLRIRFPGLPQTTLDMNKIQYNKDVGQSILESYSRV 434

Query: 639 LESLAYNIVARIEDLLYVNDLTKHSDQIPGISKLGIVAHNSSNRIQISVPFSSSPYTTKF 681
           +ES+A+NI ARI+D+LYV+D  + S  I     L + + N  N  Q +    SSP+ + F
Sbjct: 435 MESMAFNITARIDDVLYVDDAMRRS--ISVTESLSLFSINGLNP-QKAFSVQSSPHGSPF 494

BLAST of Lsi03G021540 vs. ExPASy Swiss-Prot
Match: A4IJ27 (Rop guanine nucleotide exchange factor 3 OS=Arabidopsis thaliana OX=3702 GN=ROPGEF3 PE=2 SV=1)

HSP 1 Score: 408.3 bits (1048), Expect = 1.8e-112
Identity = 230/454 (50.66%), Postives = 312/454 (68.72%), Query Frame = 0

Query: 212 NSQKMFDEKTGSTSSSDLLT-----SEETGELEEQSLRDEESSSSSEAS-MGWP------ 271
           N Q   +    ST S D        SE T    +Q   DE +S  SEAS   WP      
Sbjct: 22  NDQSAVETPVYSTMSIDSFVYPRTCSETTSGFSDQI--DETNSFCSEASPCNWPVLTESK 81

Query: 272 ----LHKTAGEGEEGVEKEVLTDSETEMMKERFSKLLLGEDMSGCGNGVCTALAISNAIT 331
               L     +  E +  + +++ E E MKERF+KLLLGEDMSG G GVCTA+ ISNAIT
Sbjct: 82  SSKCLSGLEMQSNECLVVQEISEPELETMKERFAKLLLGEDMSGSGKGVCTAVTISNAIT 141

Query: 332 NL-------------CEAERKAMWGREMELLLCVSNHIVELIPIWQTFPDGTKLEIMTCR 391
           NL              E E++A+W REM  LL V ++IVE IP  Q   +G  +E+M  R
Sbjct: 142 NLYATVFGQNLRLEPLETEKRALWKREMNCLLSVCDYIVEFIPRCQNLSNGATVEVMESR 201

Query: 392 PRSDLYVNLPALRKLDHMLLDILDSFVDSEFWYVDQGILAADQADSSS-SFRKVL-ERQD 451
           PR+D+Y+NLPALRKLD ML++ LDSF ++EFWY ++G L+   A SS+ SFRKV+ +R++
Sbjct: 202 PRADIYINLPALRKLDSMLMEALDSFQNTEFWYAEEGSLSMKSARSSTGSFRKVIVQRKE 261

Query: 452 EKWWLPIPRVPNGGLSESSTRQLHHKRDCTNQILKAAVAINSLTLADMDVPISYLESLPK 511
           EKWWLP+P VP+ GLS+ + +QL +KR+ TNQI KAA+AINS  L++M++P SY+ +LPK
Sbjct: 262 EKWWLPVPLVPSEGLSDKARKQLKNKRESTNQIHKAAMAINSSILSEMEIPDSYMTTLPK 321

Query: 512 NGRASLGEAIYKYIS-SDEFSPVLLLECLDISSEHQAIEIANRVEAAMYAWRKGIATNNS 571
            G++S+G++IY+Y+S S  F P  LL+CL+ISSEH+A+++A+RVEA+MY WR+    +NS
Sbjct: 322 CGKSSVGDSIYRYMSGSGRFFPEQLLDCLNISSEHEAVQLADRVEASMYTWRRKSCLSNS 381

Query: 572 KSSWEMLKELVID---ADKSEVLAERAELVLLCLKQQFPNLPQTSLDMSKIQYNKDVGKA 631
           K+SW M+K+L+      DK+ V+AERAE +L CLKQ++P L QTSLD+ KIQYNKDVGKA
Sbjct: 382 KNSWNMVKDLMSTTERTDKNYVMAERAETLLFCLKQRYPELSQTSLDICKIQYNKDVGKA 441

BLAST of Lsi03G021540 vs. ExPASy TrEMBL
Match: A0A5A7TMM7 (Rop guanine nucleotide exchange factor 7-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold236G004810 PE=4 SV=1)

HSP 1 Score: 1001.9 bits (2589), Expect = 1.3e-288
Identity = 524/619 (84.65%), Postives = 546/619 (88.21%), Query Frame = 0

Query: 113 MDLTFIHQQETQPIPLTNHSLKLCLPLLSRSGSDCCNPFSNLGNWVFKSARKLCQRARFS 172
           MDLTF HQQ  +PIPLTNH         S + SDC NPFSNLGNWV KSARKLC RAR S
Sbjct: 1   MDLTFTHQQHIEPIPLTNH---------SPTPSDCFNPFSNLGNWVSKSARKLCHRARSS 60

Query: 173 TGFCCRGLEYRGMVVNNSAFLEVVAMEIEGKEEKCDVFGNSQKMFDEKTGSTSSSDLLTS 232
           TGFCCRGLEYRGMVVNNSAFLEVVAMEIE KEEKCDVF N++K+ DEKT S SSSDLL S
Sbjct: 61  TGFCCRGLEYRGMVVNNSAFLEVVAMEIEAKEEKCDVFENARKVSDEKTASNSSSDLLKS 120

Query: 233 EETGELEEQSLRDEESSSSSE-----------ASMGWPLHKTAGEGEE-GVEKEVLTDSE 292
            ET E+EEQS +DEESSSSS            ASMGWPLHK  GEGEE GVEKEV TDSE
Sbjct: 121 VETEEVEEQSSKDEESSSSSSSSSSSSSSTSAASMGWPLHKRVGEGEEDGVEKEVSTDSE 180

Query: 293 TEMMKERFSKLLLGEDMSGCGNGVCTALAISNAITNLC-------------EAERKAMWG 352
            EMMKERFSKLLLGEDMSG GNGVCTALAISNAITNLC             EAERKAMWG
Sbjct: 181 MEMMKERFSKLLLGEDMSGSGNGVCTALAISNAITNLCGSVFGQLWRLEPLEAERKAMWG 240

Query: 353 REMELLLCVSNHIVELIPIWQTFPDGTKLEIMTCRPRSDLYVNLPALRKLDHMLLDILDS 412
           REME LLCVSNHIVELIPIWQTFPDGTKLEIMTCRPRSDLYVNLPALRKLDHMLLD+LDS
Sbjct: 241 REMEFLLCVSNHIVELIPIWQTFPDGTKLEIMTCRPRSDLYVNLPALRKLDHMLLDMLDS 300

Query: 413 FVDSEFWYVDQGILAADQADSSSSFRKVLERQDEKWWLPIPRVPNGGLSESSTRQLHHKR 472
           FVDSEFWY+DQGILA DQAD+SSSFRK+LERQD+KWWLPIPRVPNGGLSE+STR LHHKR
Sbjct: 301 FVDSEFWYIDQGILATDQADASSSFRKLLERQDDKWWLPIPRVPNGGLSEASTRHLHHKR 360

Query: 473 DCTNQILKAAVAINSLTLADMDVPISYLESLPKNGRASLGEAIYKYISSDEFSPVLLLEC 532
           DCTNQILKAA+AINS+TLADMDVPISYLE+LPKNGRASLGEAIYKYISSDEFSP  LLEC
Sbjct: 361 DCTNQILKAAMAINSVTLADMDVPISYLEALPKNGRASLGEAIYKYISSDEFSPAWLLEC 420

Query: 533 LDISSEHQAIEIANRVEAAMYAWR-KGIATNNSKSSWEMLKELVIDADKSEVLAERAELV 592
           LDISSEHQAIEIANRVE+AMYAWR KGIA+NNSKSSWEMLKEL+IDADKSEVLAERAELV
Sbjct: 421 LDISSEHQAIEIANRVESAMYAWRSKGIASNNSKSSWEMLKELMIDADKSEVLAERAELV 480

Query: 593 LLCLKQQFPNLPQTSLDMSKIQYNKDVGKAILESYSRVLESLAYNIVARIEDLLYVNDLT 652
           LLCLKQQFPNLPQTSLDMSKIQYNKDVGKAILESYSRVLESLAYNIVARIEDLLYVN+LT
Sbjct: 481 LLCLKQQFPNLPQTSLDMSKIQYNKDVGKAILESYSRVLESLAYNIVARIEDLLYVNELT 540

Query: 653 KHSDQIPGISKLGIVAHNSSNRIQISVPFSSSPYTTKFIKPSFSTVDLVGTRSPIPKAPQ 703
           KHSDQIPGIS+LGIVAHNSSNRIQISVPFS+SPY TKFIKPSFS VDLVGTRSP PK PQ
Sbjct: 541 KHSDQIPGISQLGIVAHNSSNRIQISVPFSTSPYNTKFIKPSFSAVDLVGTRSPTPKPPQ 600

BLAST of Lsi03G021540 vs. ExPASy TrEMBL
Match: A0A1S3B5B2 (rop guanine nucleotide exchange factor 7-like OS=Cucumis melo OX=3656 GN=LOC103486343 PE=4 SV=1)

HSP 1 Score: 1001.9 bits (2589), Expect = 1.3e-288
Identity = 524/619 (84.65%), Postives = 546/619 (88.21%), Query Frame = 0

Query: 113 MDLTFIHQQETQPIPLTNHSLKLCLPLLSRSGSDCCNPFSNLGNWVFKSARKLCQRARFS 172
           MDLTF HQQ  +PIPLTNH         S + SDC NPFSNLGNWV KSARKLC RAR S
Sbjct: 1   MDLTFTHQQHIEPIPLTNH---------SPTPSDCFNPFSNLGNWVSKSARKLCHRARSS 60

Query: 173 TGFCCRGLEYRGMVVNNSAFLEVVAMEIEGKEEKCDVFGNSQKMFDEKTGSTSSSDLLTS 232
           TGFCCRGLEYRGMVVNNSAFLEVVAMEIE KEEKCDVF N++K+ DEKT S SSSDLL S
Sbjct: 61  TGFCCRGLEYRGMVVNNSAFLEVVAMEIEAKEEKCDVFENARKVSDEKTASNSSSDLLKS 120

Query: 233 EETGELEEQSLRDEESSSSSE-----------ASMGWPLHKTAGEGEE-GVEKEVLTDSE 292
            ET E+EEQS +DEESSSSS            ASMGWPLHK  GEGEE GVEKEV TDSE
Sbjct: 121 VETEEVEEQSSKDEESSSSSSSSSSSSSSTSAASMGWPLHKRVGEGEEDGVEKEVSTDSE 180

Query: 293 TEMMKERFSKLLLGEDMSGCGNGVCTALAISNAITNLC-------------EAERKAMWG 352
            EMMKERFSKLLLGEDMSG GNGVCTALAISNAITNLC             EAERKAMWG
Sbjct: 181 MEMMKERFSKLLLGEDMSGSGNGVCTALAISNAITNLCGSVFGQLWRLEPLEAERKAMWG 240

Query: 353 REMELLLCVSNHIVELIPIWQTFPDGTKLEIMTCRPRSDLYVNLPALRKLDHMLLDILDS 412
           REME LLCVSNHIVELIPIWQTFPDGTKLEIMTCRPRSDLYVNLPALRKLDHMLLD+LDS
Sbjct: 241 REMEFLLCVSNHIVELIPIWQTFPDGTKLEIMTCRPRSDLYVNLPALRKLDHMLLDMLDS 300

Query: 413 FVDSEFWYVDQGILAADQADSSSSFRKVLERQDEKWWLPIPRVPNGGLSESSTRQLHHKR 472
           FVDSEFWY+DQGILA DQAD+SSSFRK+LERQD+KWWLPIPRVPNGGLSE+STR LHHKR
Sbjct: 301 FVDSEFWYIDQGILATDQADASSSFRKLLERQDDKWWLPIPRVPNGGLSEASTRHLHHKR 360

Query: 473 DCTNQILKAAVAINSLTLADMDVPISYLESLPKNGRASLGEAIYKYISSDEFSPVLLLEC 532
           DCTNQILKAA+AINS+TLADMDVPISYLE+LPKNGRASLGEAIYKYISSDEFSP  LLEC
Sbjct: 361 DCTNQILKAAMAINSVTLADMDVPISYLEALPKNGRASLGEAIYKYISSDEFSPAWLLEC 420

Query: 533 LDISSEHQAIEIANRVEAAMYAWR-KGIATNNSKSSWEMLKELVIDADKSEVLAERAELV 592
           LDISSEHQAIEIANRVE+AMYAWR KGIA+NNSKSSWEMLKEL+IDADKSEVLAERAELV
Sbjct: 421 LDISSEHQAIEIANRVESAMYAWRSKGIASNNSKSSWEMLKELMIDADKSEVLAERAELV 480

Query: 593 LLCLKQQFPNLPQTSLDMSKIQYNKDVGKAILESYSRVLESLAYNIVARIEDLLYVNDLT 652
           LLCLKQQFPNLPQTSLDMSKIQYNKDVGKAILESYSRVLESLAYNIVARIEDLLYVN+LT
Sbjct: 481 LLCLKQQFPNLPQTSLDMSKIQYNKDVGKAILESYSRVLESLAYNIVARIEDLLYVNELT 540

Query: 653 KHSDQIPGISKLGIVAHNSSNRIQISVPFSSSPYTTKFIKPSFSTVDLVGTRSPIPKAPQ 703
           KHSDQIPGIS+LGIVAHNSSNRIQISVPFS+SPY TKFIKPSFS VDLVGTRSP PK PQ
Sbjct: 541 KHSDQIPGISQLGIVAHNSSNRIQISVPFSTSPYNTKFIKPSFSAVDLVGTRSPTPKPPQ 600

BLAST of Lsi03G021540 vs. ExPASy TrEMBL
Match: A0A0A0LAD8 (PRONE domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G733280 PE=4 SV=1)

HSP 1 Score: 953.7 bits (2464), Expect = 4.2e-274
Identity = 508/635 (80.00%), Postives = 531/635 (83.62%), Query Frame = 0

Query: 113 MDLTFIHQQETQPIPLTNHSLKLCLPLLSRSGSDCCNPFSNLGNWVFKSARKLCQRARFS 172
           MDLTF HQQ T+PIPLTN         LS + S C NPFSNLGNWV KSARKLC R RFS
Sbjct: 1   MDLTFTHQQHTEPIPLTN---------LSPTPSVCFNPFSNLGNWVSKSARKLCHRPRFS 60

Query: 173 TGFCCRGLEYRGMVVNNSAFLEVVAMEIEGKEEKCDVFGNSQKMFDEKTGSTSSSDLLTS 232
           +GFCCRGLEYRGMVVNNSAFLEVVAMEIE KEEKCDV       FDEKT S SSSDLL  
Sbjct: 61  SGFCCRGLEYRGMVVNNSAFLEVVAMEIEVKEEKCDV-------FDEKTASISSSDLLKP 120

Query: 233 EETGELEEQSLRDEESSSSSE--------------------------------------A 292
            ET E+EE+SL+DEESSSSS                                       A
Sbjct: 121 VETEEVEERSLKDEESSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSTSAA 180

Query: 293 SMGWPLHKTAGEGEEG--VEKEVLTDSETEMMKERFSKLLLGEDMSGCGNGVCTALAISN 352
           SMGWPLH+T  EGEE   VE+EV TDSETEMMKERFSKLLLGEDMSG GNGVCTALAISN
Sbjct: 181 SMGWPLHETVVEGEEDRVVEREVSTDSETEMMKERFSKLLLGEDMSGSGNGVCTALAISN 240

Query: 353 AITNLC-------------EAERKAMWGREMELLLCVSNHIVELIPIWQTFPDGTKLEIM 412
           AITNLC             EAERKAMWGREME LLCVSNHIVELIPIWQTFPDGTKLEIM
Sbjct: 241 AITNLCGSVFGQLWRLEPLEAERKAMWGREMEFLLCVSNHIVELIPIWQTFPDGTKLEIM 300

Query: 413 TCRPRSDLYVNLPALRKLDHMLLDILDSFVDSEFWYVDQGILAADQADSSSSFRKVLERQ 472
           TCRPRSDLYVNLPALRKLDHMLLDILDSFVDSEF Y+DQGILA DQ D+SSSFRK+LERQ
Sbjct: 301 TCRPRSDLYVNLPALRKLDHMLLDILDSFVDSEFCYIDQGILATDQTDASSSFRKLLERQ 360

Query: 473 DEKWWLPIPRVPNGGLSESSTRQLHHKRDCTNQILKAAVAINSLTLADMDVPISYLESLP 532
           D+KWWLPIPRVPNGGLSE+STR L HKRDCTNQILKAA+AINS+TLADMDVPISYLE LP
Sbjct: 361 DDKWWLPIPRVPNGGLSEASTRHLQHKRDCTNQILKAAMAINSVTLADMDVPISYLEGLP 420

Query: 533 KNGRASLGEAIYKYISSDEFSPVLLLECLDISSEHQAIEIANRVEAAMYAWR-KGIATNN 592
           KNGRASLGEAIYKYISSDEFSP LLLECLDISSEHQAIEIANRVE+AMYAWR KGIA+NN
Sbjct: 421 KNGRASLGEAIYKYISSDEFSPALLLECLDISSEHQAIEIANRVESAMYAWRTKGIASNN 480

Query: 593 SKSSWEMLKELVIDADKSEVLAERAELVLLCLKQQFPNLPQTSLDMSKIQYNKDVGKAIL 652
           SKSSWEMLKEL+IDADKSEVLAERAELVLLCLKQQFPNLPQTSLDMSKIQYNKDVGKAIL
Sbjct: 481 SKSSWEMLKELMIDADKSEVLAERAELVLLCLKQQFPNLPQTSLDMSKIQYNKDVGKAIL 540

Query: 653 ESYSRVLESLAYNIVARIEDLLYVNDLTKHSDQIPGISKLGIVAHNSSNRIQISVPFSSS 694
           ESYSRVLESLAYNIVARIEDLLYVN+LTKHSDQIPGIS+LGIVAHNSSNRI IS+PFS+S
Sbjct: 541 ESYSRVLESLAYNIVARIEDLLYVNELTKHSDQIPGISQLGIVAHNSSNRIHISMPFSTS 600

BLAST of Lsi03G021540 vs. ExPASy TrEMBL
Match: A0A5D3DP03 (Rop guanine nucleotide exchange factor 7-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G001280 PE=4 SV=1)

HSP 1 Score: 941.4 bits (2432), Expect = 2.1e-270
Identity = 502/619 (81.10%), Postives = 524/619 (84.65%), Query Frame = 0

Query: 113 MDLTFIHQQETQPIPLTNHSLKLCLPLLSRSGSDCCNPFSNLGNWVFKSARKLCQRARFS 172
           MDLTF HQQ  +PIPLTNH         S + SDC NPFSNLGNWV KSARKLC RAR S
Sbjct: 1   MDLTFTHQQHIEPIPLTNH---------SPTPSDCFNPFSNLGNWVSKSARKLCHRARSS 60

Query: 173 TGFCCRGLEYRGMVVNNSAFLEVVAMEIEGKEEKCDVFGNSQKMFDEKTGSTSSSDLLTS 232
           TGFCCRGLEYRGMVVNNSAFLEVVAMEIE KEEKCDVF N++K+ DEKT S SSSDLL S
Sbjct: 61  TGFCCRGLEYRGMVVNNSAFLEVVAMEIEAKEEKCDVFENARKVSDEKTASNSSSDLLKS 120

Query: 233 EETGELEEQSLRDEESSSSSE-----------ASMGWPLHKTAGEGEE-GVEKEVLTDSE 292
            ET E+EEQS +DEESSSSS            ASMGWPLHK  GEGEE GVEKEV TDSE
Sbjct: 121 VETEEVEEQSSKDEESSSSSSSSSSSSSSTSAASMGWPLHKRVGEGEEDGVEKEVSTDSE 180

Query: 293 TEMMKERFSKLLLGEDMSGCGNGVCTALAISNAITNLC-------------EAERKAMWG 352
            EMMKERFSKLLLGEDMSG GNGVCTALAISNAITNLC             EAERKAMWG
Sbjct: 181 MEMMKERFSKLLLGEDMSGSGNGVCTALAISNAITNLCGSVFGQLWRLEPLEAERKAMWG 240

Query: 353 REMELLLCVSNHIVELIPIWQTFPDGTKLEIMTCRPRSDLYVNLPALRKLDHMLLDILDS 412
           REME LLC                      IMTCRPRSDLYVNLPALRKLDHMLLD+LDS
Sbjct: 241 REMEFLLC----------------------IMTCRPRSDLYVNLPALRKLDHMLLDMLDS 300

Query: 413 FVDSEFWYVDQGILAADQADSSSSFRKVLERQDEKWWLPIPRVPNGGLSESSTRQLHHKR 472
           FVDSEFWY+DQGILA DQAD+SSSFRK+LERQD+KWWLPIPRVPNGGLSE+STR LHHKR
Sbjct: 301 FVDSEFWYIDQGILATDQADASSSFRKLLERQDDKWWLPIPRVPNGGLSEASTRHLHHKR 360

Query: 473 DCTNQILKAAVAINSLTLADMDVPISYLESLPKNGRASLGEAIYKYISSDEFSPVLLLEC 532
           DCTNQILKAA+AINS+TLADMDVPISYLE+LPKNGRASLGEAIYKYISSDEFSP  LLEC
Sbjct: 361 DCTNQILKAAMAINSVTLADMDVPISYLEALPKNGRASLGEAIYKYISSDEFSPAWLLEC 420

Query: 533 LDISSEHQAIEIANRVEAAMYAWR-KGIATNNSKSSWEMLKELVIDADKSEVLAERAELV 592
           LDISSEHQAIEIANRVE+AMYAWR KGIA+NNSKSSWEMLKEL+IDADKSEVLAERAELV
Sbjct: 421 LDISSEHQAIEIANRVESAMYAWRSKGIASNNSKSSWEMLKELMIDADKSEVLAERAELV 480

Query: 593 LLCLKQQFPNLPQTSLDMSKIQYNKDVGKAILESYSRVLESLAYNIVARIEDLLYVNDLT 652
           LLCLKQQFPNLPQTSLDMSKIQYNKDVGKAILESYSRVLESLAYNIVARIEDLLYVN+LT
Sbjct: 481 LLCLKQQFPNLPQTSLDMSKIQYNKDVGKAILESYSRVLESLAYNIVARIEDLLYVNELT 540

Query: 653 KHSDQIPGISKLGIVAHNSSNRIQISVPFSSSPYTTKFIKPSFSTVDLVGTRSPIPKAPQ 703
           KHSDQIPGIS+LGIVAHNSSNRIQISVPFS+SPY TKFIKPSFS VDLVGTRSP PK PQ
Sbjct: 541 KHSDQIPGISQLGIVAHNSSNRIQISVPFSTSPYNTKFIKPSFSAVDLVGTRSPTPKPPQ 588

BLAST of Lsi03G021540 vs. ExPASy TrEMBL
Match: A0A6J1CWY5 (rop guanine nucleotide exchange factor 7-like isoform X2 OS=Momordica charantia OX=3673 GN=LOC111015048 PE=4 SV=1)

HSP 1 Score: 810.4 bits (2092), Expect = 5.7e-231
Identity = 445/610 (72.95%), Postives = 489/610 (80.16%), Query Frame = 0

Query: 113 MDLTFIHQ-QETQPIPLTNHSLKLCLPLLSRSGSDCCNPFSNLGNWVFKSARKLCQRARF 172
           MD+ F HQ QE  PI   NHSLKLCLP L     DC NPFS L  WV+KSARK+     F
Sbjct: 1   MDVNFAHQEQERVPIRFRNHSLKLCLPNL--VNHDCSNPFSILRRWVWKSARKV-----F 60

Query: 173 STGFCCRGLEYRGMVVNNS------AFLEVVAMEI-----EGKEEKCDVFGNSQKMFDEK 232
            +GFCCRGLEY+GMVVNNS      AFLE VAMEI     EGKEEKCD+FGN+ K+FDEK
Sbjct: 61  PSGFCCRGLEYKGMVVNNSTFCSSPAFLEEVAMEIRVEDKEGKEEKCDMFGNAHKVFDEK 120

Query: 233 TG-----STSSSDLLTSEETGELEEQSLRDEESSSSSEASMGWPLHKTAGEGEEG----- 292
            G     S+SSSDLLTSE TG        DEESSSS+   MGWPL++T GE   G     
Sbjct: 121 RGDGESASSSSSDLLTSEATGS------HDEESSSST--LMGWPLNQTVGEEGGGDKLHF 180

Query: 293 ----VEKEVLTDSETEMMKERFSKLLLGEDMSGCGNGVCTALAISNAITNLC-------- 352
               +E+E  + SE E+M ERFSKLLLGEDMSGCGNGV TALAISNAITNLC        
Sbjct: 181 DDRELEREASSVSEMEIM-ERFSKLLLGEDMSGCGNGVSTALAISNAITNLCATLFGQLW 240

Query: 353 -----EAERKAMWGREMELLLCVSNHIVELIPIWQTFPDGTKLEIMTCRPRSDLYVNLPA 412
                 AERK MW REME LLCVSNHIVELIP+WQTFPDG+KLEIMTCRPRSDLY NLPA
Sbjct: 241 RLEPLAAERKGMWRREMEWLLCVSNHIVELIPVWQTFPDGSKLEIMTCRPRSDLYANLPA 300

Query: 413 LRKLDHMLLDILDSFVDSEFWYVDQGILAADQADSSSSFRKVLERQDEKWWLPIPRVPNG 472
           LRKLDHMLLDILDSFVDSEFWYVDQGILAA+     SSFRK+LERQ++KWWLP+PRVP+G
Sbjct: 301 LRKLDHMLLDILDSFVDSEFWYVDQGILAAEA--DGSSFRKLLERQEDKWWLPVPRVPSG 360

Query: 473 GLSESSTRQLHHKRDCTNQILKAAVAINSLTLADMDVPISYLESLPKNGRASLGEAIYKY 532
           GLSE+S RQL HKRDCTNQILKAA+AINS+TLADMDVP SYLE+LPKNGRASLGE IY++
Sbjct: 361 GLSEASRRQLQHKRDCTNQILKAAMAINSVTLADMDVPDSYLEALPKNGRASLGEGIYRH 420

Query: 533 ISSDEFSPVLLLECLDISSEHQAIEIANRVEAAMYAWRKGIA---TNNS-----KSSWEM 592
           ISSDEFSPV LLECLD+SSEHQAIEIANRVEAAMYAWRKG+    TNNS     KSSWEM
Sbjct: 421 ISSDEFSPVCLLECLDMSSEHQAIEIANRVEAAMYAWRKGMGSKPTNNSLRSSAKSSWEM 480

Query: 593 LKELVIDADKSEVLAERAELVLLCLKQQFPNLPQTSLDMSKIQYNKDVGKAILESYSRVL 652
           LKELVIDADKSE+ AERAE VL CLKQQFPNLPQT+LDMSKIQ+NKDVGKAILESYSRVL
Sbjct: 481 LKELVIDADKSELFAERAESVLYCLKQQFPNLPQTNLDMSKIQHNKDVGKAILESYSRVL 540

Query: 653 ESLAYNIVARIEDLLYVNDLTKHSDQIPGISKLGIVAHNSSNRIQISVPFSSSPYTTKFI 676
           ESLA+NIVARI+DLLYV+DL KHSD+IP IS+LGIVAHN+SNRIQ SVPFSSSPYTT F 
Sbjct: 541 ESLAFNIVARIDDLLYVDDLAKHSDEIPRISQLGIVAHNTSNRIQFSVPFSSSPYTTTFT 592

BLAST of Lsi03G021540 vs. NCBI nr
Match: XP_038905547.1 (rop guanine nucleotide exchange factor 7-like isoform X2 [Benincasa hispida])

HSP 1 Score: 1035.8 bits (2677), Expect = 1.7e-298
Identity = 534/611 (87.40%), Postives = 557/611 (91.16%), Query Frame = 0

Query: 113 MDLTFIHQQETQPIPLTNHSLKLCLPLLSRSGSDCCNPFSNLGNWVFKSARKLCQRARFS 172
           MDLTF HQQ+T+PIPLTNH         S + SDC NPFSNLG WV KSARKLCQ+ARFS
Sbjct: 1   MDLTFTHQQDTEPIPLTNH---------SPTRSDCFNPFSNLGYWVSKSARKLCQKARFS 60

Query: 173 TGFCCRGLEYRGMVVNNSAFLEVVAMEIEGKEEKCDVFGNSQKMFDEKTGSTSSSDLLTS 232
           TGFCCRGLEYRGMVVNNSAFLEVVAMEIEGKEEKCDVFGNSQK+FDE TGSTSSSDLLTS
Sbjct: 61  TGFCCRGLEYRGMVVNNSAFLEVVAMEIEGKEEKCDVFGNSQKVFDENTGSTSSSDLLTS 120

Query: 233 EETGELEEQSLRDEESSSSSE-----ASMGWPLHKTAGEGEEGVEKEVLTDSETEMMKER 292
           +ETGE+EEQ ++DEESSSSS      ASMGWPLHKT GEGEEGVE+E+ TDSETEMMKER
Sbjct: 121 KETGEMEEQRMQDEESSSSSSSSSSAASMGWPLHKTGGEGEEGVEREISTDSETEMMKER 180

Query: 293 FSKLLLGEDMSGCGNGVCTALAISNAITNLC-------------EAERKAMWGREMELLL 352
           FSKLLLGEDMSGCGNGV TALAISNAITNLC             EAERKAMW REMELLL
Sbjct: 181 FSKLLLGEDMSGCGNGVSTALAISNAITNLCGSVFGQLWRLEPLEAERKAMWEREMELLL 240

Query: 353 CVSNHIVELIPIWQTFPDGTKLEIMTCRPRSDLYVNLPALRKLDHMLLDILDSFVDSEFW 412
           CVSNHIVELIPIWQTFPDGTKLEIMTCRPRSDLYVNLPALRKLDHMLL ILDSFVDSEFW
Sbjct: 241 CVSNHIVELIPIWQTFPDGTKLEIMTCRPRSDLYVNLPALRKLDHMLLGILDSFVDSEFW 300

Query: 413 YVDQGILAADQADSSSSFRKVLERQDEKWWLPIPRVPNGGLSESSTRQLHHKRDCTNQIL 472
           YVDQGILAADQ D+SSSFRKVLERQDEKWWLPIPRVPNGGLSESSTRQLHHKRDCTNQIL
Sbjct: 301 YVDQGILAADQVDASSSFRKVLERQDEKWWLPIPRVPNGGLSESSTRQLHHKRDCTNQIL 360

Query: 473 KAAVAINSLTLADMDVPISYLESLPKNGRASLGEAIYKYISSDEFSPVLLLECLDISSEH 532
           KAA+AINSLTLA+MD+PISYLESLPKNGRASLGEAIYK+ISSDEFSPV LLECLDISSEH
Sbjct: 361 KAAMAINSLTLAEMDIPISYLESLPKNGRASLGEAIYKHISSDEFSPVWLLECLDISSEH 420

Query: 533 QAIEIANRVEAAMYAWRKGIATNNSKSSWEMLKELVIDADKSEVLAERAELVLLCLKQQF 592
           QAIEIANRVE AMY WRKGIA+NNSKSSWEMLKELVID DK EVLAERAELVLLCLKQ+F
Sbjct: 421 QAIEIANRVEGAMYVWRKGIASNNSKSSWEMLKELVIDGDKIEVLAERAELVLLCLKQRF 480

Query: 593 PNLPQTSLDMSKIQYNKDVGKAILESYSRVLESLAYNIVARIEDLLYVNDLTKHSDQIPG 652
           PNLPQTSLDMSKIQYNKDVGKAILESYSRVLESLAYNIVARIEDLLYVN++TKHSDQIPG
Sbjct: 481 PNLPQTSLDMSKIQYNKDVGKAILESYSRVLESLAYNIVARIEDLLYVNEMTKHSDQIPG 540

Query: 653 ISKLGIVAHNSSNRIQISVPFSSSPYTTKFIKPSFSTVDLVGTRSPIPKAPQCGLEE--- 703
           IS+LGIVAHNSSNRIQISVPFSSSPYTTKFI PSFSTVDLVGTRS IPKAPQCGLEE   
Sbjct: 541 ISQLGIVAHNSSNRIQISVPFSSSPYTTKFINPSFSTVDLVGTRSLIPKAPQCGLEESKK 600

BLAST of Lsi03G021540 vs. NCBI nr
Match: XP_038905546.1 (rop guanine nucleotide exchange factor 7-like isoform X1 [Benincasa hispida])

HSP 1 Score: 1019.2 bits (2634), Expect = 1.7e-293
Identity = 534/643 (83.05%), Postives = 557/643 (86.63%), Query Frame = 0

Query: 113 MDLTFIHQQETQPIPLTNHSLKLCLPLLSRSGSDCCNPFSNLGNWVFKSARKLCQRARFS 172
           MDLTF HQQ+T+PIPLTNH         S + SDC NPFSNLG WV KSARKLCQ+ARFS
Sbjct: 1   MDLTFTHQQDTEPIPLTNH---------SPTRSDCFNPFSNLGYWVSKSARKLCQKARFS 60

Query: 173 TGFCCRGLEYRGMVVNNSAFLEVVAMEIEGKEEKCDVFGNSQKMFDEKTGSTSSSDLLTS 232
           TGFCCRGLEYRGMVVNNSAFLEVVAMEIEGKEEKCDVFGNSQK+FDE TGSTSSSDLLTS
Sbjct: 61  TGFCCRGLEYRGMVVNNSAFLEVVAMEIEGKEEKCDVFGNSQKVFDENTGSTSSSDLLTS 120

Query: 233 EETGELEEQSLRDEESSSSSE-----ASMGWPLHKTAGEGEEGVEKEVLTDSETEMMKER 292
           +ETGE+EEQ ++DEESSSSS      ASMGWPLHKT GEGEEGVE+E+ TDSETEMMKER
Sbjct: 121 KETGEMEEQRMQDEESSSSSSSSSSAASMGWPLHKTGGEGEEGVEREISTDSETEMMKER 180

Query: 293 FSKLLLGEDMSGCGNGVCTALAISNAITNLC-------------EAERKAMWGREMELLL 352
           FSKLLLGEDMSGCGNGV TALAISNAITNLC             EAERKAMW REMELLL
Sbjct: 181 FSKLLLGEDMSGCGNGVSTALAISNAITNLCGSVFGQLWRLEPLEAERKAMWEREMELLL 240

Query: 353 CVSNHIVELIPIWQTFPDGTKLE--------------------------------IMTCR 412
           CVSNHIVELIPIWQTFPDGTKLE                                IMTCR
Sbjct: 241 CVSNHIVELIPIWQTFPDGTKLEVQFNSIQFITKFSFPLYLQFFHVLLSLAHHHQIMTCR 300

Query: 413 PRSDLYVNLPALRKLDHMLLDILDSFVDSEFWYVDQGILAADQADSSSSFRKVLERQDEK 472
           PRSDLYVNLPALRKLDHMLL ILDSFVDSEFWYVDQGILAADQ D+SSSFRKVLERQDEK
Sbjct: 301 PRSDLYVNLPALRKLDHMLLGILDSFVDSEFWYVDQGILAADQVDASSSFRKVLERQDEK 360

Query: 473 WWLPIPRVPNGGLSESSTRQLHHKRDCTNQILKAAVAINSLTLADMDVPISYLESLPKNG 532
           WWLPIPRVPNGGLSESSTRQLHHKRDCTNQILKAA+AINSLTLA+MD+PISYLESLPKNG
Sbjct: 361 WWLPIPRVPNGGLSESSTRQLHHKRDCTNQILKAAMAINSLTLAEMDIPISYLESLPKNG 420

Query: 533 RASLGEAIYKYISSDEFSPVLLLECLDISSEHQAIEIANRVEAAMYAWRKGIATNNSKSS 592
           RASLGEAIYK+ISSDEFSPV LLECLDISSEHQAIEIANRVE AMY WRKGIA+NNSKSS
Sbjct: 421 RASLGEAIYKHISSDEFSPVWLLECLDISSEHQAIEIANRVEGAMYVWRKGIASNNSKSS 480

Query: 593 WEMLKELVIDADKSEVLAERAELVLLCLKQQFPNLPQTSLDMSKIQYNKDVGKAILESYS 652
           WEMLKELVID DK EVLAERAELVLLCLKQ+FPNLPQTSLDMSKIQYNKDVGKAILESYS
Sbjct: 481 WEMLKELVIDGDKIEVLAERAELVLLCLKQRFPNLPQTSLDMSKIQYNKDVGKAILESYS 540

Query: 653 RVLESLAYNIVARIEDLLYVNDLTKHSDQIPGISKLGIVAHNSSNRIQISVPFSSSPYTT 703
           RVLESLAYNIVARIEDLLYVN++TKHSDQIPGIS+LGIVAHNSSNRIQISVPFSSSPYTT
Sbjct: 541 RVLESLAYNIVARIEDLLYVNEMTKHSDQIPGISQLGIVAHNSSNRIQISVPFSSSPYTT 600

BLAST of Lsi03G021540 vs. NCBI nr
Match: XP_008442490.1 (PREDICTED: rop guanine nucleotide exchange factor 7-like [Cucumis melo] >KAA0044128.1 rop guanine nucleotide exchange factor 7-like [Cucumis melo var. makuwa])

HSP 1 Score: 1001.9 bits (2589), Expect = 2.7e-288
Identity = 524/619 (84.65%), Postives = 546/619 (88.21%), Query Frame = 0

Query: 113 MDLTFIHQQETQPIPLTNHSLKLCLPLLSRSGSDCCNPFSNLGNWVFKSARKLCQRARFS 172
           MDLTF HQQ  +PIPLTNH         S + SDC NPFSNLGNWV KSARKLC RAR S
Sbjct: 1   MDLTFTHQQHIEPIPLTNH---------SPTPSDCFNPFSNLGNWVSKSARKLCHRARSS 60

Query: 173 TGFCCRGLEYRGMVVNNSAFLEVVAMEIEGKEEKCDVFGNSQKMFDEKTGSTSSSDLLTS 232
           TGFCCRGLEYRGMVVNNSAFLEVVAMEIE KEEKCDVF N++K+ DEKT S SSSDLL S
Sbjct: 61  TGFCCRGLEYRGMVVNNSAFLEVVAMEIEAKEEKCDVFENARKVSDEKTASNSSSDLLKS 120

Query: 233 EETGELEEQSLRDEESSSSSE-----------ASMGWPLHKTAGEGEE-GVEKEVLTDSE 292
            ET E+EEQS +DEESSSSS            ASMGWPLHK  GEGEE GVEKEV TDSE
Sbjct: 121 VETEEVEEQSSKDEESSSSSSSSSSSSSSTSAASMGWPLHKRVGEGEEDGVEKEVSTDSE 180

Query: 293 TEMMKERFSKLLLGEDMSGCGNGVCTALAISNAITNLC-------------EAERKAMWG 352
            EMMKERFSKLLLGEDMSG GNGVCTALAISNAITNLC             EAERKAMWG
Sbjct: 181 MEMMKERFSKLLLGEDMSGSGNGVCTALAISNAITNLCGSVFGQLWRLEPLEAERKAMWG 240

Query: 353 REMELLLCVSNHIVELIPIWQTFPDGTKLEIMTCRPRSDLYVNLPALRKLDHMLLDILDS 412
           REME LLCVSNHIVELIPIWQTFPDGTKLEIMTCRPRSDLYVNLPALRKLDHMLLD+LDS
Sbjct: 241 REMEFLLCVSNHIVELIPIWQTFPDGTKLEIMTCRPRSDLYVNLPALRKLDHMLLDMLDS 300

Query: 413 FVDSEFWYVDQGILAADQADSSSSFRKVLERQDEKWWLPIPRVPNGGLSESSTRQLHHKR 472
           FVDSEFWY+DQGILA DQAD+SSSFRK+LERQD+KWWLPIPRVPNGGLSE+STR LHHKR
Sbjct: 301 FVDSEFWYIDQGILATDQADASSSFRKLLERQDDKWWLPIPRVPNGGLSEASTRHLHHKR 360

Query: 473 DCTNQILKAAVAINSLTLADMDVPISYLESLPKNGRASLGEAIYKYISSDEFSPVLLLEC 532
           DCTNQILKAA+AINS+TLADMDVPISYLE+LPKNGRASLGEAIYKYISSDEFSP  LLEC
Sbjct: 361 DCTNQILKAAMAINSVTLADMDVPISYLEALPKNGRASLGEAIYKYISSDEFSPAWLLEC 420

Query: 533 LDISSEHQAIEIANRVEAAMYAWR-KGIATNNSKSSWEMLKELVIDADKSEVLAERAELV 592
           LDISSEHQAIEIANRVE+AMYAWR KGIA+NNSKSSWEMLKEL+IDADKSEVLAERAELV
Sbjct: 421 LDISSEHQAIEIANRVESAMYAWRSKGIASNNSKSSWEMLKELMIDADKSEVLAERAELV 480

Query: 593 LLCLKQQFPNLPQTSLDMSKIQYNKDVGKAILESYSRVLESLAYNIVARIEDLLYVNDLT 652
           LLCLKQQFPNLPQTSLDMSKIQYNKDVGKAILESYSRVLESLAYNIVARIEDLLYVN+LT
Sbjct: 481 LLCLKQQFPNLPQTSLDMSKIQYNKDVGKAILESYSRVLESLAYNIVARIEDLLYVNELT 540

Query: 653 KHSDQIPGISKLGIVAHNSSNRIQISVPFSSSPYTTKFIKPSFSTVDLVGTRSPIPKAPQ 703
           KHSDQIPGIS+LGIVAHNSSNRIQISVPFS+SPY TKFIKPSFS VDLVGTRSP PK PQ
Sbjct: 541 KHSDQIPGISQLGIVAHNSSNRIQISVPFSTSPYNTKFIKPSFSAVDLVGTRSPTPKPPQ 600

BLAST of Lsi03G021540 vs. NCBI nr
Match: XP_004137923.3 (rop guanine nucleotide exchange factor 7 isoform X1 [Cucumis sativus])

HSP 1 Score: 959.9 bits (2480), Expect = 1.2e-275
Identity = 508/619 (82.07%), Postives = 531/619 (85.78%), Query Frame = 0

Query: 113 MDLTFIHQQETQPIPLTNHSLKLCLPLLSRSGSDCCNPFSNLGNWVFKSARKLCQRARFS 172
           MDLTF HQQ T+PIPLTN         LS + S C NPFSNLGNWV KSARKLC R RFS
Sbjct: 1   MDLTFTHQQHTEPIPLTN---------LSPTPSVCFNPFSNLGNWVSKSARKLCHRPRFS 60

Query: 173 TGFCCRGLEYRGMVVNNSAFLEVVAMEIEGKEEKCDVFGNSQKMFDEKTGSTSSSDLLTS 232
           +GFCCRGLEYRGMVVNNSAFLEVVAMEIE KEEKCDV       FDEKT S SSSDLL  
Sbjct: 61  SGFCCRGLEYRGMVVNNSAFLEVVAMEIEVKEEKCDV-------FDEKTASISSSDLLKP 120

Query: 233 EETGELEEQSLRDEESSSSSE----------------------ASMGWPLHKTAGEGEEG 292
            ET E+EE+SL+DEESSSSS                       ASMGWPLH+T  EGEE 
Sbjct: 121 VETEEVEERSLKDEESSSSSSSSSSSSSSSSSSSSSSSSSTSAASMGWPLHETVVEGEED 180

Query: 293 --VEKEVLTDSETEMMKERFSKLLLGEDMSGCGNGVCTALAISNAITNLC---------- 352
             VE+EV TDSETEMMKERFSKLLLGEDMSG GNGVCTALAISNAITNLC          
Sbjct: 181 RVVEREVSTDSETEMMKERFSKLLLGEDMSGSGNGVCTALAISNAITNLCGSVFGQLWRL 240

Query: 353 ---EAERKAMWGREMELLLCVSNHIVELIPIWQTFPDGTKLEIMTCRPRSDLYVNLPALR 412
              EAERKAMWGREME LLCVSNHIVELIPIWQTFPDGTKLEIMTCRPRSDLYVNLPALR
Sbjct: 241 EPLEAERKAMWGREMEFLLCVSNHIVELIPIWQTFPDGTKLEIMTCRPRSDLYVNLPALR 300

Query: 413 KLDHMLLDILDSFVDSEFWYVDQGILAADQADSSSSFRKVLERQDEKWWLPIPRVPNGGL 472
           KLDHMLLDILDSFVDSEF Y+DQGILA DQ D+SSSFRK+LERQD+KWWLPIPRVPNGGL
Sbjct: 301 KLDHMLLDILDSFVDSEFCYIDQGILATDQTDASSSFRKLLERQDDKWWLPIPRVPNGGL 360

Query: 473 SESSTRQLHHKRDCTNQILKAAVAINSLTLADMDVPISYLESLPKNGRASLGEAIYKYIS 532
           SE+STR L HKRDCTNQILKAA+AINS+TLADMDVPISYLE LPKNGRASLGEAIYKYIS
Sbjct: 361 SEASTRHLQHKRDCTNQILKAAMAINSVTLADMDVPISYLEGLPKNGRASLGEAIYKYIS 420

Query: 533 SDEFSPVLLLECLDISSEHQAIEIANRVEAAMYAWR-KGIATNNSKSSWEMLKELVIDAD 592
           SDEFSP LLLECLDISSEHQAIEIANRVE+AMYAWR KGIA+NNSKSSWEMLKEL+IDAD
Sbjct: 421 SDEFSPALLLECLDISSEHQAIEIANRVESAMYAWRTKGIASNNSKSSWEMLKELMIDAD 480

Query: 593 KSEVLAERAELVLLCLKQQFPNLPQTSLDMSKIQYNKDVGKAILESYSRVLESLAYNIVA 652
           KSEVLAERAELVLLCLKQQFPNLPQTSLDMSKIQYNKDVGKAILESYSRVLESLAYNIVA
Sbjct: 481 KSEVLAERAELVLLCLKQQFPNLPQTSLDMSKIQYNKDVGKAILESYSRVLESLAYNIVA 540

Query: 653 RIEDLLYVNDLTKHSDQIPGISKLGIVAHNSSNRIQISVPFSSSPYTTKFIKPSFSTVDL 694
           RIEDLLYVN+LTKHSDQIPGIS+LGIVAHNSSNRI IS+PFS+SPY T FIKPSFS+VDL
Sbjct: 541 RIEDLLYVNELTKHSDQIPGISQLGIVAHNSSNRIHISMPFSTSPYNTNFIKPSFSSVDL 600

BLAST of Lsi03G021540 vs. NCBI nr
Match: XP_011651877.2 (rop guanine nucleotide exchange factor 7 isoform X2 [Cucumis sativus])

HSP 1 Score: 951.0 bits (2457), Expect = 5.6e-273
Identity = 506/619 (81.74%), Postives = 529/619 (85.46%), Query Frame = 0

Query: 113 MDLTFIHQQETQPIPLTNHSLKLCLPLLSRSGSDCCNPFSNLGNWVFKSARKLCQRARFS 172
           MDLTF HQQ T+PIPLTN         LS + S C NPFSNLGNWV KSARKLC R RFS
Sbjct: 1   MDLTFTHQQHTEPIPLTN---------LSPTPSVCFNPFSNLGNWVSKSARKLCHRPRFS 60

Query: 173 TGFCCRGLEYRGMVVNNSAFLEVVAMEIEGKEEKCDVFGNSQKMFDEKTGSTSSSDLLTS 232
           +GFCCRGLEYRGMVVNNSAFLEVVAMEIE KEEKCDV       FDEKT S SSSDLL  
Sbjct: 61  SGFCCRGLEYRGMVVNNSAFLEVVAMEIEVKEEKCDV-------FDEKTASISSSDLLKP 120

Query: 233 EETGELEEQSLRDEESSSSSE----------------------ASMGWPLHKTAGEGEEG 292
            ET E+EE+SL+DEESSSSS                       ASMGWPLH+T  EGEE 
Sbjct: 121 VETEEVEERSLKDEESSSSSSSSSSSSSSSSSSSSSSSSSTSAASMGWPLHETVVEGEED 180

Query: 293 --VEKEVLTDSETEMMKERFSKLLLGEDMSGCGNGVCTALAISNAITNLC---------- 352
             VE+EV TDS  EMMKERFSKLLLGEDMSG GNGVCTALAISNAITNLC          
Sbjct: 181 RVVEREVSTDS--EMMKERFSKLLLGEDMSGSGNGVCTALAISNAITNLCGSVFGQLWRL 240

Query: 353 ---EAERKAMWGREMELLLCVSNHIVELIPIWQTFPDGTKLEIMTCRPRSDLYVNLPALR 412
              EAERKAMWGREME LLCVSNHIVELIPIWQTFPDGTKLEIMTCRPRSDLYVNLPALR
Sbjct: 241 EPLEAERKAMWGREMEFLLCVSNHIVELIPIWQTFPDGTKLEIMTCRPRSDLYVNLPALR 300

Query: 413 KLDHMLLDILDSFVDSEFWYVDQGILAADQADSSSSFRKVLERQDEKWWLPIPRVPNGGL 472
           KLDHMLLDILDSFVDSEF Y+DQGILA DQ D+SSSFRK+LERQD+KWWLPIPRVPNGGL
Sbjct: 301 KLDHMLLDILDSFVDSEFCYIDQGILATDQTDASSSFRKLLERQDDKWWLPIPRVPNGGL 360

Query: 473 SESSTRQLHHKRDCTNQILKAAVAINSLTLADMDVPISYLESLPKNGRASLGEAIYKYIS 532
           SE+STR L HKRDCTNQILKAA+AINS+TLADMDVPISYLE LPKNGRASLGEAIYKYIS
Sbjct: 361 SEASTRHLQHKRDCTNQILKAAMAINSVTLADMDVPISYLEGLPKNGRASLGEAIYKYIS 420

Query: 533 SDEFSPVLLLECLDISSEHQAIEIANRVEAAMYAWR-KGIATNNSKSSWEMLKELVIDAD 592
           SDEFSP LLLECLDISSEHQAIEIANRVE+AMYAWR KGIA+NNSKSSWEMLKEL+IDAD
Sbjct: 421 SDEFSPALLLECLDISSEHQAIEIANRVESAMYAWRTKGIASNNSKSSWEMLKELMIDAD 480

Query: 593 KSEVLAERAELVLLCLKQQFPNLPQTSLDMSKIQYNKDVGKAILESYSRVLESLAYNIVA 652
           KSEVLAERAELVLLCLKQQFPNLPQTSLDMSKIQYNKDVGKAILESYSRVLESLAYNIVA
Sbjct: 481 KSEVLAERAELVLLCLKQQFPNLPQTSLDMSKIQYNKDVGKAILESYSRVLESLAYNIVA 540

Query: 653 RIEDLLYVNDLTKHSDQIPGISKLGIVAHNSSNRIQISVPFSSSPYTTKFIKPSFSTVDL 694
           RIEDLLYVN+LTKHSDQIPGIS+LGIVAHNSSNRI IS+PFS+SPY T FIKPSFS+VDL
Sbjct: 541 RIEDLLYVNELTKHSDQIPGISQLGIVAHNSSNRIHISMPFSTSPYNTNFIKPSFSSVDL 600

BLAST of Lsi03G021540 vs. TAIR 10
Match: AT5G02010.1 (RHO guanyl-nucleotide exchange factor 7 )

HSP 1 Score: 551.6 bits (1420), Expect = 9.2e-157
Identity = 299/479 (62.42%), Postives = 362/479 (75.57%), Query Frame = 0

Query: 211 GNSQKMFDEKTGSTSSSDLLTSEETGELEEQSLRDEESSSSSEASMGWPLHKTAGEGEEG 270
           G+S+ + + +     SS   +     E EEQS    E  ++S  S  W +    GE    
Sbjct: 3   GSSENLPEVEEKGRESSCCSSETTRQEEEEQSPSCTEDFTASPVSSRWSVKNIDGE---- 62

Query: 271 VEKEVLTD---SETEMMKERFSKLLLGEDMSGCGNGVCTALAISNAITNLC--------- 330
            +K++ +D   SE EMMKERFSKLLLGEDMSG GNGVCTALAISNAITNLC         
Sbjct: 63  -KKKIRSDSRVSEVEMMKERFSKLLLGEDMSGSGNGVCTALAISNAITNLCATLFGQLWR 122

Query: 331 ----EAERKAMWGREMELLLCVSNHIVELIPIWQTFPDGTKLEIMTCRPRSDLYVNLPAL 390
                 E+K MW REME LLCVS+HIVE+ P WQTFPDGTKLEIMTCRPRSDLYVNLPAL
Sbjct: 123 LEPLPTEKKEMWRREMEWLLCVSDHIVEMTPTWQTFPDGTKLEIMTCRPRSDLYVNLPAL 182

Query: 391 RKLDHMLLDILDSFVDSEFWYVDQGILAADQ-ADSSSSFRKVLERQDEKWWLPIPRVPNG 450
           RKLD+MLL+ILDSF ++EFWYVDQGI+A +  AD SSSFRK  +RQ++KWWLP+PRV  G
Sbjct: 183 RKLDNMLLEILDSFEETEFWYVDQGIMAHESAADGSSSFRKSFQRQEDKWWLPVPRVSPG 242

Query: 451 GLSESSTRQLHHKRDCTNQILKAAVAINSLTLADMDVPISYLESLPKNGRASLGEAIYKY 510
           GL E+S +QL HKRDCTNQILKAA+AINS+TLADM++P SYLESLP+ GR+ LG+ IY+Y
Sbjct: 243 GLQENSRKQLQHKRDCTNQILKAAMAINSITLADMEIPESYLESLPRKGRSCLGDLIYRY 302

Query: 511 ISSDEFSPVLLLECLDISSEHQAIEIANRVEAAMYAWRK---GIATNNSKSSWEMLKELV 570
           ISSD+FSP  LL+CLD+SSEHQAIEIANRVE+++Y W K        N+K+SWEM+KEL+
Sbjct: 303 ISSDQFSPECLLDCLDLSSEHQAIEIANRVESSIYLWHKRTNSKPATNTKTSWEMVKELM 362

Query: 571 IDADKSEVLAERAELVLLCLKQQFPNLPQTSLDMSKIQYNKDVGKAILESYSRVLESLAY 630
           +DADK E++A+RAE +LL LKQ+FP LPQT+LDMSKIQYNKD+GK+ILESYSRVLESLA+
Sbjct: 363 VDADKLELMADRAESLLLSLKQRFPGLPQTALDMSKIQYNKDIGKSILESYSRVLESLAF 422

Query: 631 NIVARIEDLLYVNDLTKH-SDQIPGISKLGIVAHNSSNRIQISVPFSSSPYTTKFIKPS 669
           NIVARI+DLL+V+DLT+H SDQIP  + LG    N+ N    S+    S YTT    PS
Sbjct: 423 NIVARIDDLLFVDDLTRHSSDQIP--TTLG----NNGNDAPKSIAVPVSNYTTPSYSPS 470

BLAST of Lsi03G021540 vs. TAIR 10
Match: AT5G05940.1 (ROP guanine nucleotide exchange factor 5 )

HSP 1 Score: 482.3 bits (1240), Expect = 6.8e-136
Identity = 270/523 (51.63%), Postives = 355/523 (67.88%), Query Frame = 0

Query: 177 CRGLEYRGMVVNNSAFLEVVAMEIEGKEEKCDVFGNSQKMFDEKTGSTSSSDLLTSEETG 236
           C G+E +    N +  +E +  E    +E+    G S +     T ++SS     S+  G
Sbjct: 8   CAGIEKKRS--NLTPSMEDILTE---SKERSSTSGASYESSSTTTVASSSPPPPPSQILG 67

Query: 237 ELEEQSLRDEESSSSSEASMGWPLHKTAGEGEEGVEKEVLTDSETEMMKERFSKLLLGED 296
                 +R      +S+ S+ +   K     + G + + +  ++ EMMKERF+KLLLGED
Sbjct: 68  ----WPIRKASFRKNSKESVNFDHKKLTLHDDSGFKAKEMNSADVEMMKERFAKLLLGED 127

Query: 297 MSGCGNGVCTALAISNAITNLC-------------EAERKAMWGREMELLLCVSNHIVEL 356
           MSG G GVCTALAISNAITNLC              +E+K MW REME +L VS+HIVEL
Sbjct: 128 MSGSGKGVCTALAISNAITNLCATIFGQLWRLEPLSSEKKEMWRREMEWILSVSDHIVEL 187

Query: 357 IPIWQTFPDGTKLEIMTCRPRSDLYVNLPALRKLDHMLLDILDSFVDSEFWYVDQGILAA 416
            P  QT+PDG K E+MTCRPR DL++NLPALRKLD+MLLDIL SF  +EFWYVDQGI+A+
Sbjct: 188 TPSTQTYPDGNKFEVMTCRPRFDLFINLPALRKLDNMLLDILASFKKTEFWYVDQGIVAS 247

Query: 417 DQADSSSSFRKVLERQDEKWWLPIPRVPNGGLSESSTRQLHHKRDCTNQILKAAVAINSL 476
           +  D S+SFR+ ++RQ+EKWWLP+PR+   GL+E +  +L+HKR+C  QILKAA+AINSL
Sbjct: 248 EN-DGSASFRRKIQRQEEKWWLPVPRLAPNGLTEEARTELNHKRECATQILKAAMAINSL 307

Query: 477 TLADMDVPISYLESLPKNGRASLGEAIYKYISSDEFSPVLLLECLDISSEHQAIEIANRV 536
            L +MDVP SYLE+LPKNGR+ LG+ IY+Y++SD+FS   LL+CLD+SSEH A++IANRV
Sbjct: 308 ALTEMDVPKSYLETLPKNGRSCLGDVIYRYVTSDKFSAESLLDCLDLSSEHIALDIANRV 367

Query: 537 EAAMYAWRKGIAT----NNSKSS------WEMLKELVIDADKSEVLAERAELVLLCLKQQ 596
           EA++Y WR+ + T    NN+ SS      WEM+KEL+   DK  +L ER+E +L CLKQ+
Sbjct: 368 EASIYVWRRRVQTKLGVNNNTSSTTPKLTWEMVKELMAAGDKRGLLVERSETLLRCLKQR 427

Query: 597 FPNLPQTSLDMSKIQYNKDVGKAILESYSRVLESLAYNIVARIEDLLYVNDLTKHSD--- 656
           FP+L QTSLD+SKIQ+NKD+GK+ILESYSR LESLA NI+ARI+DLLYV+DLTK SD   
Sbjct: 428 FPSLTQTSLDISKIQWNKDIGKSILESYSRALESLASNIIARIDDLLYVDDLTKQSDDNN 487

Query: 657 --QIPGISKLGIVAHNSSNRIQISVPFSSSPYTTKF-IKPSFS 671
               P +S   I+AH     +   +  S +PY T F   P FS
Sbjct: 488 LLSSPAVS--SIIAHKKVVPLPYLISASGTPYRTSFSTTPGFS 518

BLAST of Lsi03G021540 vs. TAIR 10
Match: AT3G55660.1 (ROP (rho of plants) guanine nucleotide exchange factor 6 )

HSP 1 Score: 420.6 bits (1080), Expect = 2.4e-117
Identity = 258/538 (47.96%), Postives = 351/538 (65.24%), Query Frame = 0

Query: 210 FGNSQK----MFDEKTGSTSSSDLLTSEE-TGELEEQSLRDEESSSSSEAS---MGWPL- 269
           FG + K    + D  T ST+ S L +S    G    +S   E S SS       +GWPL 
Sbjct: 15  FGETSKRIIGLIDSVTESTTDSSLSSSSSGVGSSSGRSSVAERSVSSPPTKSQILGWPLG 74

Query: 270 ----HKTAGEGEE---------GVEKEVLTDSETEMMKERFSKLLLGEDMSGCGNGVCTA 329
                K++G+ ++         G ++     SE E++KER +KLLLGEDMSG G GVC A
Sbjct: 75  QGSWRKSSGKMKKKTPTKIDDFGFKRVGTETSEIELLKERMAKLLLGEDMSGSGEGVCPA 134

Query: 330 LAISNAITNLCEA-------------ERKAMWGREMELLLCVSNHIVELIPIWQTFPDGT 389
           LAISNAITNL  A             E+K MW RE+E+LL VS+HIVEL+P +Q FP+G 
Sbjct: 135 LAISNAITNLYAAILGQQWRLEPIPSEKKLMWRREIEVLLSVSDHIVELVPSFQNFPNGN 194

Query: 390 KLEIMTCRPRSDLYVNLPALRKLDHMLLDILDSFVDSEFWYVDQGILAADQADSSSSFRK 449
           K+E+M CRPRSDL+  LPALRKLD+ML++ILDSF ++EFWYVDQGI+AA+ A  S+SFR+
Sbjct: 195 KIEVMNCRPRSDLFTCLPALRKLDNMLIEILDSFGETEFWYVDQGIVAAESA-RSNSFRE 254

Query: 450 VLERQDEKWWLPIPRVPNGGLSESSTRQLHHKRDCTNQILKAAVAINSLTLADMDVPISY 509
                 +KWWLP+PRVP+ GL+E + ++L H R+ TNQILKA ++INS+ LA+M+VP SY
Sbjct: 255 ----DGDKWWLPLPRVPSDGLTEQTRKKLDHTREFTNQILKACMSINSIALAEMEVPQSY 314

Query: 510 LESLPKNGRASLGEAIYKYISSDEFSPVLLLECLDISSEHQAIEIANRVEAAMYAWRKGI 569
           LE+LPKNGR+ LG+ +Y+ I+SD FS   LLE +D+SSE   +E+ANRVEA+MY WR+  
Sbjct: 315 LEALPKNGRSCLGDFLYRNITSDNFSADHLLESIDLSSELAVVEMANRVEASMYVWRRRA 374

Query: 570 AT-------NNSKSSWEML-KELVI---DADKSEVLAERAELVLLCLKQQFPNLPQTSLD 629
            +        ++ + W M+ KE+++   D DK E+ AERAE +L+ LKQ+FP L QT+LD
Sbjct: 375 HSRHLISLYRSTSTRWGMIVKEMMMHQTDGDKREIFAERAESLLIRLKQRFPGLRQTALD 434

Query: 630 MSKIQYNKDVGKAILESYSRVLESLAYNIVARIEDLLYVNDLTKH---------SDQIPG 682
            SKIQYNKDVGK+ILESYSRVLESLAY+I  RIE++L+++D++K          SD++  
Sbjct: 435 TSKIQYNKDVGKSILESYSRVLESLAYSIGVRIEEVLFMDDISKDDGDGDDDSCSDKLRL 494

BLAST of Lsi03G021540 vs. TAIR 10
Match: AT4G38430.1 (rho guanyl-nucleotide exchange factor 1 )

HSP 1 Score: 410.2 bits (1053), Expect = 3.3e-114
Identity = 246/496 (49.60%), Postives = 322/496 (64.92%), Query Frame = 0

Query: 219 EKTGSTS-SSDLLTSEETGELEEQSLRDEESSSSSEAS----------MGWPLHKTAGEG 278
           E+ GS S S+D+  SE +          E +SSS  +S             P+      G
Sbjct: 15  ERCGSYSPSADISESESSSSFSCHRFDGEGASSSIPSSPRVVAGRGFYFPAPVMLPVIGG 74

Query: 279 EEGVEKEVLTD---SETEMMKERFSKLLLGEDMSGCGNGVCTALAISNAITNLCEA---- 338
           ++ V  +   D   SE EMMKERF+KLLLGEDMSG G GVCTALAISNAITNL       
Sbjct: 75  KDVVWDDKQPDNDLSEIEMMKERFAKLLLGEDMSGGGKGVCTALAISNAITNLSATVFGE 134

Query: 339 ---------ERKAMWGREMELLLCVSNHIVELIPIWQTFPDGTKLEIMTCRPRSDLYVNL 398
                    ++KAMW RE+E LLCVS+ IVELIP  Q FP G   EIM  RPRSDLY NL
Sbjct: 135 LWRLEPLAPQKKAMWRRELEWLLCVSDSIVELIPSIQQFPGGGTYEIMETRPRSDLYANL 194

Query: 399 PALRKLDHMLLDILDSFVDSEFWYVDQGILAADQADSSSSFRKVLERQDEKWWLPIPRVP 458
           PAL+KLD ML+D+LD+F D+EFWY D+GI+  D  D  S       RQ++KWWLP P+VP
Sbjct: 195 PALKKLDAMLIDMLDAFSDTEFWYTDRGIVLGD-CDKDSYNSPASVRQEDKWWLPCPKVP 254

Query: 459 NGGLSESSTRQLHHKRDCTNQILKAAVAINSLTLADMDVPISYLESLPKNGRASLGEAIY 518
             GLSE + ++L   RD  NQILKAA+AINS  LA+M++P  YLE+LPK+G+  LGE IY
Sbjct: 255 PNGLSEEARKKLQQCRDFANQILKAALAINSGVLAEMEIPDPYLETLPKSGKECLGEIIY 314

Query: 519 KYISSDEFSPVLLLECLDISSEHQAIEIANRVEAAMYAWRKGIATNNSK------SSW-E 578
           +Y+++++FSP  LL+CLD+SSEHQ +EIANR+EAA++ WR+     + K      SSW  
Sbjct: 315 QYLTANKFSPECLLDCLDLSSEHQTLEIANRIEAAVHVWRQKNGRRHKKQAKLKLSSWGG 374

Query: 579 MLKELVIDADKSEVLAERAELVLLCLKQQFPNLPQTSLDMSKIQYNKDVGKAILESYSRV 638
            +K LV D ++++ L +RAE +L  L+ +FP LPQT+LDM+KIQYNKDVG++ILESYSRV
Sbjct: 375 KVKGLVNDNERNDFLVQRAETLLQSLRIRFPGLPQTTLDMNKIQYNKDVGQSILESYSRV 434

Query: 639 LESLAYNIVARIEDLLYVNDLTKHSDQIPGISKLGIVAHNSSNRIQISVPFSSSPYTTKF 681
           +ES+A+NI ARI+D+LYV+D  + S  I     L + + N  N  Q +    SSP+ + F
Sbjct: 435 MESMAFNITARIDDVLYVDDAMRRS--ISVTESLSLFSINGLNP-QKAFSVQSSPHGSPF 494

BLAST of Lsi03G021540 vs. TAIR 10
Match: AT4G00460.2 (RHO guanyl-nucleotide exchange factor 3 )

HSP 1 Score: 408.3 bits (1048), Expect = 1.3e-113
Identity = 230/454 (50.66%), Postives = 312/454 (68.72%), Query Frame = 0

Query: 212 NSQKMFDEKTGSTSSSDLLT-----SEETGELEEQSLRDEESSSSSEAS-MGWP------ 271
           N Q   +    ST S D        SE T    +Q   DE +S  SEAS   WP      
Sbjct: 22  NDQSAVETPVYSTMSIDSFVYPRTCSETTSGFSDQI--DETNSFCSEASPCNWPVLTESK 81

Query: 272 ----LHKTAGEGEEGVEKEVLTDSETEMMKERFSKLLLGEDMSGCGNGVCTALAISNAIT 331
               L     +  E +  + +++ E E MKERF+KLLLGEDMSG G GVCTA+ ISNAIT
Sbjct: 82  SSKCLSGLEMQSNECLVVQEISEPELETMKERFAKLLLGEDMSGSGKGVCTAVTISNAIT 141

Query: 332 NL-------------CEAERKAMWGREMELLLCVSNHIVELIPIWQTFPDGTKLEIMTCR 391
           NL              E E++A+W REM  LL V ++IVE IP  Q   +G  +E+M  R
Sbjct: 142 NLYATVFGQNLRLEPLETEKRALWKREMNCLLSVCDYIVEFIPRCQNLSNGATVEVMESR 201

Query: 392 PRSDLYVNLPALRKLDHMLLDILDSFVDSEFWYVDQGILAADQADSSS-SFRKVL-ERQD 451
           PR+D+Y+NLPALRKLD ML++ LDSF ++EFWY ++G L+   A SS+ SFRKV+ +R++
Sbjct: 202 PRADIYINLPALRKLDSMLMEALDSFQNTEFWYAEEGSLSMKSARSSTGSFRKVIVQRKE 261

Query: 452 EKWWLPIPRVPNGGLSESSTRQLHHKRDCTNQILKAAVAINSLTLADMDVPISYLESLPK 511
           EKWWLP+P VP+ GLS+ + +QL +KR+ TNQI KAA+AINS  L++M++P SY+ +LPK
Sbjct: 262 EKWWLPVPLVPSEGLSDKARKQLKNKRESTNQIHKAAMAINSSILSEMEIPDSYMTTLPK 321

Query: 512 NGRASLGEAIYKYIS-SDEFSPVLLLECLDISSEHQAIEIANRVEAAMYAWRKGIATNNS 571
            G++S+G++IY+Y+S S  F P  LL+CL+ISSEH+A+++A+RVEA+MY WR+    +NS
Sbjct: 322 CGKSSVGDSIYRYMSGSGRFFPEQLLDCLNISSEHEAVQLADRVEASMYTWRRKSCLSNS 381

Query: 572 KSSWEMLKELVID---ADKSEVLAERAELVLLCLKQQFPNLPQTSLDMSKIQYNKDVGKA 631
           K+SW M+K+L+      DK+ V+AERAE +L CLKQ++P L QTSLD+ KIQYNKDVGKA
Sbjct: 382 KNSWNMVKDLMSTTERTDKNYVMAERAETLLFCLKQRYPELSQTSLDICKIQYNKDVGKA 441