Lsi03G019650 (gene) Bottle gourd (USVL1VR-Ls) v1

BLAST of Lsi03G019650 vs. ExPASy Swiss-Prot
Match: Q9LZD0 (Purine-uracil permease NCS1 OS=Arabidopsis thaliana OX=3702 GN=NCS1 PE=1 SV=1)

HSP 1 Score: 716.1 bits (1847), Expect = 3.1e-205
Identity = 355/517 (68.67%), Postives = 424/517 (82.01%), Query Frame = 0

Query: 37  FSPSTP--KLLLKRRSNL--KFTAPMASPV-DHPSEDTQNDDS-GREDLRPTTPEQRTLS 96
           FSP  P   L+L R   +  + +A   S + DH  +++Q D S   +DL+PTTP QRT S
Sbjct: 74  FSPILPGDNLVLSRSGVIRPRLSAMTGSEINDHGYDESQFDPSLTNDDLKPTTPSQRTFS 133

Query: 97  WLEMALLWIGLIVGVPAYYLAGSLVDLGMSWWQGIATVVAANAVTLIALILTGHPGTKYG 156
           WL+M+ LWIGL+VGVP YYLAGSLVDLGM+WWQGIATVV AN + L+ L+LT  PGT YG
Sbjct: 134 WLDMSSLWIGLVVGVPTYYLAGSLVDLGMAWWQGIATVVTANLILLVPLVLTAQPGTLYG 193

Query: 157 ISFPVFARSSFGIHGAHIPTLLRALIGCGWYGIETWIAGEAIFLLLPESIKKSSFSQVLP 216
           ISFPV ARSSFGI GAHIPTLLRAL+GCGWYGIETWI GEAIFLLLP  IKKS+ S  LP
Sbjct: 194 ISFPVLARSSFGIRGAHIPTLLRALVGCGWYGIETWIGGEAIFLLLPGHIKKSALSHTLP 253

Query: 217 WLGTSPLAFTCFLAFWVVQLASVWKGMDGIRKLETFSAPILVFLTVCLLIWSCVRAGGFG 276
           WLGTSPL F+CF+ FW+ QL  VW+GMDGIRKLE +SAPIL+ LT CLL WS ++AGGFG
Sbjct: 254 WLGTSPLEFSCFIVFWLAQLCIVWRGMDGIRKLEKYSAPILISLTSCLLAWSYLKAGGFG 313

Query: 277 HMLSLTSRLSSSEFWALFFPSLTANISFWATLALNIPDFTRYAKSQKDQIIGQLGLPIFM 336
           HMLSL+S+L+S++FW LFFPSLTANISFWATLALNIPDF+R+AKSQ DQIIGQ+GLP+FM
Sbjct: 314 HMLSLSSKLTSAQFWTLFFPSLTANISFWATLALNIPDFSRFAKSQTDQIIGQVGLPVFM 373

Query: 337 GAFTFVGVAVTSSTGVIFGHIISNPIELLGHIGGFATKLLAIFGIILATVTTNVAANVVA 396
           G FTFVGVAVTSST +IFG +ISNPIELLG IGG AT LLAI GI LAT+TTN+AANVVA
Sbjct: 374 GLFTFVGVAVTSSTSIIFGRVISNPIELLGQIGGLATTLLAIVGISLATLTTNIAANVVA 433

Query: 397 PANALVNLSPSYFTFTRGALLTAVIGILFQPWRLLSSSESFVYTWLVGYSALLGPIGGVM 456
           PANALVNL+P +FTF RGA LTAV+GI+FQPWRLL SSESFVYTWL+GYSALLGPIGG++
Sbjct: 434 PANALVNLNPKFFTFGRGAFLTAVLGIVFQPWRLLKSSESFVYTWLIGYSALLGPIGGII 493

Query: 457 VTDYYLIRRRELSVEDLYSTSPTGAYYYSGGFNLAAVVALVVGVLPVIPGFLEKVEIVSK 516
           + DYYLI++ +L++ DLYS SP+G YY+S G+N+AAVVALV G++PV+PGFL K+  +SK
Sbjct: 494 LVDYYLIKKMKLNIGDLYSLSPSGEYYFSKGYNVAAVVALVAGIIPVVPGFLHKISALSK 553

Query: 517 VPKIFGIIYGNAWFISTFLAGFCYWGLSVTLKRRKVS 548
           +   F ++Y NA F S  +AGF YW +   L R++ S
Sbjct: 554 ISNGFVVVYDNALFFSFIIAGFVYWIIMSRLGRKQSS 590

BLAST of Lsi03G019650 vs. ExPASy Swiss-Prot
Match: P94575 (Probable allantoin permease OS=Bacillus subtilis (strain 168) OX=224308 GN=pucI PE=2 SV=1)

HSP 1 Score: 290.4 bits (742), Expect = 4.2e-77
Identity = 170/469 (36.25%), Postives = 258/469 (55.01%), Query Frame = 0

Query: 70  QNDDSGREDLRPTTPEQRTLSWLEMALLWIGLIVGVPAYYLAGSLVDLGMSWWQGIATVV 129
           Q++    EDL P   E+RT   +  A +W+G I  +P Y   G L+ +G+S WQ +A ++
Sbjct: 9   QSNRLSNEDLVPLGQEKRTWKAMNFASIWMGCIHNIPTYATVGGLIAIGLSPWQVLAIII 68

Query: 130 AANAVTLIALILTGHPGTKYGISFPVFARSSFGIHGAHIPTLLRALIGCGWYGIETWIAG 189
            A+ +   AL L GH GTKYG+ FPV  R+S+GI+GA+IP LLRA     W GI+T+   
Sbjct: 69  TASLILFGALALNGHAGTKYGLPFPVIIRASYGIYGANIPALLRAFTAIMWLGIQTFAGS 128

Query: 190 EAI-FLLLPESIKKSSFSQVLPWLGTSPLAFTCFLAFWVVQLASVWKGMDGIRKLETFSA 249
            A+  LLL               LG        F+ FW + L  +  GM+ I++ E ++ 
Sbjct: 129 TALNILLLNMWPGWGEIGGEWNILGIHLSGLLSFVFFWAIHLLVLHHGMESIKRFEVWAG 188

Query: 250 PILVFLTVCLLIWSCVRAGGFGHMLSLTSRLS--SSEFWALFFPSLTANISFWATLALNI 309
           P++  +   ++ W+   AGG G + S   +    S  FW  F   +T  I  WATL LNI
Sbjct: 189 PLVYLVFGGMVWWAVDIAGGLGPIYSQPGKFHTFSETFWP-FAAGVTGIIGIWATLILNI 248

Query: 310 PDFTRYAKSQKDQIIGQL-GLPIFMGAFTFVGVAVTSSTGVIFGHIISNPIELLGHIGGF 369
           PDFTR+A++QK+QI GQ  GLP     F F  + VTS + V FG  I + +++L      
Sbjct: 249 PDFTRFAETQKEQIKGQFYGLPGTFALFAFASITVTSGSQVAFGEPIWDVVDILARFDNP 308

Query: 370 ATKLLAIFGIILATVTTNVAANVVAPANALVNLSPSYFTFTRGALLTAVIGILFQPWRLL 429
              +L++  + +AT++ NVAAN+V+PA  + N  P Y  F RG+ +TA++ +   PW+L+
Sbjct: 309 YVIVLSVITLCIATISVNVAANIVSPAYDIANALPKYINFKRGSFITALLALFTVPWKLM 368

Query: 430 SSSESFVYTWLVGYSALLGPIGGVMVTDYYLIRRRELSVEDLYSTSPTGAYYYSGGFNLA 489
            S+ S VY +L     +LGP+ GVM+ DY++IR+RELSV+DLY  S TG Y Y  G+N  
Sbjct: 369 ESATS-VYAFLGLIGGMLGPVAGVMMADYFIIRKRELSVDDLY--SETGRYVYWKGYNYR 428

Query: 490 AVVALVVGVLPVIPGFLEKVEIVSKVPKIFGIIYGNAWFISTFLAGFCY 535
           A  A ++G L         + ++     +   +Y  +WF+   ++   Y
Sbjct: 429 AFAATMLGAL---------ISLIGMYVPVLKSLYDISWFVGVLISFLFY 464

BLAST of Lsi03G019650 vs. ExPASy Swiss-Prot
Match: O14035 (Uncharacterized permease C29B12.14c OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPAC29B12.14c PE=3 SV=2)

HSP 1 Score: 235.0 bits (598), Expect = 2.1e-60
Identity = 154/449 (34.30%), Postives = 232/449 (51.67%), Query Frame = 0

Query: 77  EDLRPTTPEQRTLSWLEMALLWIGLIVGVPAYYLAGSLVDLGMSWWQGIATVVAANAVTL 136
           EDL P    QR          W+   V V  + +AG+ V+ G+SWW+   TV     +  
Sbjct: 51  EDLYPVPKSQRVWGPWNYVAFWLADSVNVNTWMIAGTAVESGLSWWEAWITVWVGYTIAA 110

Query: 137 IALILTGHPGTKYGISFPVFARSSFGIHGAHIPTLLRALIGCGWYGIETWIAGEAIFLLL 196
             L + G  G  Y ISFPV +RSSFGI G+  P L RA++ C WYG++ WI GE + L++
Sbjct: 111 FILTIAGRAGAVYHISFPVLSRSSFGIWGSLWPILNRAVMACVWYGVQAWIGGECVTLMI 170

Query: 197 PESIKKSSFSQV---LPWLGTSPLAFTCFLAFWVVQLASVWKGMDGIRKLETFSAPILVF 256
             SI   SFS +   +   GT    +  F  FW++   ++W  +  IR L T  + +   
Sbjct: 171 -RSI-WPSFSHIPNTMAKSGTETYQWVGFFIFWLISNVAIWFPVYQIRHLFTAKSFLAPP 230

Query: 257 LTVCLLIWSCVRAGGFGHMLSLTSRLSS-SEFWALFFPSLTANISFWATLALNIPDFTRY 316
             +  LIW+ V+A G G  +   ++LS+ +  WA+    + + +  +ATL +N PDFTR+
Sbjct: 231 AAIAFLIWALVKAHGAGDAIHAKTQLSTWNHGWAV-TAGIISCLDNFATLIVNNPDFTRF 290

Query: 317 AKSQKDQIIGQL-GLPIFMGAFTFVGVAVTSSTGVIFGHIISNPIELL-------GHIGG 376
           A +    I  QL  +P+  G  T +GV V S++  I+G  I NP++LL        H G 
Sbjct: 291 ATTPNAPIFPQLITIPMGFGITTLIGVLVGSASKSIYGENIWNPLDLLKSFLDHSNHHGV 350

Query: 377 FATKLLAIFGIILATVTTNVAANVVAPANALVNLSPSYFTFTRGALLTAVIGILFQPWRL 436
            A       G+ LA +  N+AAN V+  N    L P +    RG  + ++IGI   PW L
Sbjct: 351 RAGVFFISTGLCLAQLGVNIAANTVSAGNDTSALCPMFINIRRGGYIASIIGICMCPWNL 410

Query: 437 LSSSESFVYTWLVGYSALLGPIGGVMVTDYYLIRRRELSVEDLYSTSPTGAYYYSGGFNL 496
           LSSS SF  + L  Y+  L    G+++ DY++IR+  L V+ LY+ +P   Y+++ G NL
Sbjct: 411 LSSSNSFANS-LSAYAVFLSSFAGILIADYFVIRKGYLKVDALYTINPNEPYWFTYGINL 470

Query: 497 AAVVALVVGVLPVIPGFLEKVEIVSKVPK 514
            A  + + G+L  + G    V    KVPK
Sbjct: 471 RAFASYICGLLINVVGLAGAVG--DKVPK 493

BLAST of Lsi03G019650 vs. ExPASy Swiss-Prot
Match: Q10279 (Uracil permease OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=fur4 PE=1 SV=2)

HSP 1 Score: 224.2 bits (570), Expect = 3.7e-57
Identity = 144/465 (30.97%), Postives = 233/465 (50.11%), Query Frame = 0

Query: 77  EDLRPTTPEQRTLSWLEMALLWIGLIVGVPAYYLAGSLVDLGMSWWQGIATVVAANAVTL 136
           +DL P  P +R          W+   V +  + ++ + ++LG++WW+    V     +  
Sbjct: 44  KDLYPVPPSKRLWGPWNFISFWLADAVNINTWMISATAIELGLNWWEAWICVWVGYLICG 103

Query: 137 IALILTGHPGTKYGISFPVFARSSFGIHGAHIPTLLRALIGCGWYGIETWIAGEAIFLLL 196
           I +  TG PG  Y ISFPV +RSSFG  G+  P L R+++ C WYG++ WI GE + L++
Sbjct: 104 ILVATTGRPGAVYHISFPVLSRSSFGTWGSLWPILNRSVLACVWYGVQAWIGGECVVLMI 163

Query: 197 PESIKKSSFSQV---LPWLGTSPLAFTCFLAFWVVQLASVWKGMDGIRKLETFSAPILVF 256
             SI   SFS +   +   GT    +  F  FW++   ++W  +  IR L TF A +   
Sbjct: 164 -RSI-WPSFSHIPNTMAKSGTETYQWVGFFIFWLLSNIAIWFPVHQIRHLFTFKAIVAPP 223

Query: 257 LTVCLLIWSCVRAGGFGHMLSLTSRLSSSEFWALFFPSLTANISFWATLALNIPDFTRYA 316
             +  LIW+ V+A G G ++   ++L   E   +    +   I  +ATL +N PDF R+A
Sbjct: 224 AAIAFLIWALVKAHGAGPVIHEPTKLGQYEHAWVVINGIVTCIDGFATLIVNNPDFARFA 283

Query: 317 KSQKDQIIGQ-LGLPIFMGAFTFVGVAVTSSTGVIFGHIISNPIELLGHI-------GGF 376
            +       Q + +P+  G  + +GV V+S++  I+G  + +P +LL          G  
Sbjct: 284 TTPGAVHWPQIITVPLAFGVTSLIGVLVSSASKAIYGTTLWDPTQLLASFLDHSNAHGVR 343

Query: 377 ATKLLAIFGIILATVTTNVAANVVAPANALVNLSPSYFTFTRGALLTAVIGILFQPWRLL 436
           A      FG+ +A +  N+AAN V+  N L  L P+     RG  + ++I +   PW LL
Sbjct: 344 AGVFFIAFGLCIAQLGVNIAANSVSAGNDLSALLPTVINVRRGGYIASIIALCMCPWNLL 403

Query: 437 SSSESFVYTWLVGYSALLGPIGGVMVTDYYLIRRRELSVEDLYSTSPTGAYYYSGGFNLA 496
           SS+ +F  T+L  YS  L    GV++ DYY +R+  + V  LYS+S +  YY+  G N  
Sbjct: 404 SSNNNFT-TYLSSYSVFLSSFAGVIIADYYFVRKGLIRVAPLYSSSSSSPYYFWKGINFR 463

Query: 497 AVVALVVGVLPVIPGFLEKVEIVSKVPKIFGIIYGNAWFISTFLA 531
           A  + + G+L  I G         KVPK+   ++   +F+   +A
Sbjct: 464 AFASYICGMLINIVGMAGSTG--QKVPKVANTMFNLNYFLGITVA 503

BLAST of Lsi03G019650 vs. ExPASy Swiss-Prot
Match: Q04895 (Allantoin permease OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=DAL4 PE=1 SV=1)

HSP 1 Score: 197.2 bits (500), Expect = 4.9e-49
Identity = 136/472 (28.81%), Postives = 229/472 (48.52%), Query Frame = 0

Query: 78  DLRPTTPEQRTLSWLEMALLWIGLIVGVPAYYLAGSLVDLGMSWWQGIATVVAANAVTLI 137
           DL+P   E+R  SW      W+     +  + +AG+ + LG++WWQ   TV        I
Sbjct: 102 DLKPVEEERRCWSWFNYLYFWLADCFNINTWQIAGTGLQLGLNWWQCWLTVWIGYTFAGI 161

Query: 138 ALILTGHPGTKYGISFPVFARSSFGIHGAHIPTLLRALIGCGWYGIETWIAGEAIFLLLP 197
            ++L    G+ Y +SFP+  R+SFGI  +  P + R ++   WY ++ W+    + L+L 
Sbjct: 162 FVVLNSRFGSAYHLSFPITVRASFGIFFSMWPIINRVVMAIVWYAVQAWLGATPVALMLK 221

Query: 198 ESIKKSSFSQVLPWLG---TSPLAFTCFLAFWVVQLASVWKGMDGIRKLETFSAPILVFL 257
               K+   ++    G   ++   F CF  FWVV +  V      IR L T  A ++ F 
Sbjct: 222 SIFGKNLEDRIPNHFGSPNSTTFEFMCFFIFWVVSIPFVLVAPHKIRHLFTVKAALIPFA 281

Query: 258 TVCLLIWSCVRAGGFGHMLSLTS-RLSSSEFWALFFPSLTANISFWATLALNIPDFTRYA 317
               LIW+  ++ G   + +L       SEF  +F  SL A ++ +A L +N PDF R+A
Sbjct: 282 AFGFLIWALKKSHGKIELGTLNDYSPHGSEFSWIFVRSLMACVANFAALIINAPDFGRFA 341

Query: 318 KSQKDQIIGQL-GLPIFMGAFTFVGVAVTSSTGVIFGHIISNPIELLGHI-------GGF 377
           K+ +  +  QL  +P+F      +G+ VT++   ++G    +P+++LG         G  
Sbjct: 342 KNPQASLWPQLVAIPLFFAITCLIGIIVTAAGYHLYGVNYWSPLDVLGQFLETTYTRGTR 401

Query: 378 ATKLLAIFGIILATVTTNVAANVVAPANALVNLSPSYFTFTRGALLTAVIGILFQPWRLL 437
           A   L  F   LA + TN++AN +A    +  L P Y    RG+L    + +   PW L+
Sbjct: 402 AGVFLISFVFALAQLGTNISANSLACGADMTALFPRYINIRRGSLFCVAMALCICPWNLM 461

Query: 438 SSSESFVYTWLVGYSALLGPIGGVMVTDYYLIRRRELSVEDLYSTSPTGAYYYSG---GF 497
           +SS  F  + L  Y+  L  I GV+  DY+++RR  + +  L+  +  G++Y  G   G 
Sbjct: 462 ASSSKFT-SALGAYAIFLSSIAGVICADYFVVRRGYVKLTHLF-LAQKGSFYMFGNKFGA 521

Query: 498 NLAAVVALVVGVLPVIPGFL-----EKVEIVSKVPKIFGIIYGNAWFISTFL 530
           N  A VA + G+ P +PGF+      K+ +     +++ + Y   +FIS  +
Sbjct: 522 NWRAFVAYICGIAPNLPGFIGDVGAPKITVSEGAMRLYYLGYPVGFFISAVI 571

BLAST of Lsi03G019650 vs. ExPASy TrEMBL
Match: A0A5D3DPI8 (Purine-uracil permease NCS1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G003170 PE=3 SV=1)

HSP 1 Score: 1015.8 bits (2625), Expect = 7.0e-293
Identity = 512/554 (92.42%), Postives = 530/554 (95.67%), Query Frame = 0

Query: 1   MVTQSINLHLHHHIFHLPPSLSALRHRPIVSQSPLPFSPSTPKLLLKRRSNLKFTAPMAS 60
           MVT  INLHLH HIFHLPPS+S LR+RP VS SPL FSP TPKLLLKRRSNL+FT+PMAS
Sbjct: 1   MVTHCINLHLHRHIFHLPPSISTLRYRPTVSHSPLLFSPITPKLLLKRRSNLRFTSPMAS 60

Query: 61  PVDHPSEDTQNDDSGREDLRPTTPEQRTLSWLEMALLWIGLIVGVPAYYLAGSLVDLGMS 120
              +PSEDTQNDDSG++DLRPTT EQRTLSW EMALLWIGLIVGVP+YYLAGSLVDLGMS
Sbjct: 61  ADANPSEDTQNDDSGKDDLRPTTLEQRTLSWPEMALLWIGLIVGVPSYYLAGSLVDLGMS 120

Query: 121 WWQGIATVVAANAVTLIALILTGHPGTKYGISFPVFARSSFGIHGAHIPTLLRALIGCGW 180
           WWQGIATVV ANAVTLIALIL GHPGTKYGISFPVFARSSFGIHGAHIPTLLRALIGCGW
Sbjct: 121 WWQGIATVVVANAVTLIALILNGHPGTKYGISFPVFARSSFGIHGAHIPTLLRALIGCGW 180

Query: 181 YGIETWIAGEAIFLLLPESIKKSSFSQVLPWLGTSPLAFTCFLAFWVVQLASVWKGMDGI 240
           YGIETWIAGEAIFLLLPESIKKSSFSQ+LPWLGTSPLAFTCFLAFWVVQLASVWKGMDGI
Sbjct: 181 YGIETWIAGEAIFLLLPESIKKSSFSQILPWLGTSPLAFTCFLAFWVVQLASVWKGMDGI 240

Query: 241 RKLETFSAPILVFLTVCLLIWSCVRAGGFGHMLSLTSRLSSSEFWALFFPSLTANISFWA 300
           RKLETFSAPILVFLTVCLLIWSCV+AGGFGHMLSLTS+LSSSEFWALFFPSLTANISFWA
Sbjct: 241 RKLETFSAPILVFLTVCLLIWSCVKAGGFGHMLSLTSKLSSSEFWALFFPSLTANISFWA 300

Query: 301 TLALNIPDFTRYAKSQKDQIIGQLGLPIFMGAFTFVGVAVTSSTGVIFGHIISNPIELLG 360
           TLALNIPDFTRYAKSQKDQIIGQLGLPIFMGAFTFVGVAVTSSTGVIFGHIISNPI+LLG
Sbjct: 301 TLALNIPDFTRYAKSQKDQIIGQLGLPIFMGAFTFVGVAVTSSTGVIFGHIISNPIDLLG 360

Query: 361 HIGGFATKLLAIFGIILATVTTNVAANVVAPANALVNLSPSYFTFTRGALLTAVIGILFQ 420
           HIGGF TK+LAIFGIILATVTTNVAANVVAPANALVNLSPSYFTFTRGALLTAVIGILFQ
Sbjct: 361 HIGGFVTKVLAIFGIILATVTTNVAANVVAPANALVNLSPSYFTFTRGALLTAVIGILFQ 420

Query: 421 PWRLLSSSESFVYTWLVGYSALLGPIGGVMVTDYYLIRRRELSVEDLYSTSPTGAYYYSG 480
           PWRLLSSSESFVYTWLVGYSALLGPIGGVMVTDYYLIR +ELSVEDLYSTSP GAYYYSG
Sbjct: 421 PWRLLSSSESFVYTWLVGYSALLGPIGGVMVTDYYLIRGKELSVEDLYSTSPRGAYYYSG 480

Query: 481 GFNLAAVVALVVGVLPVIPGFLEKVEIVSKVPKIFGIIYGNAWFISTFLAGFCYWGLSVT 540
           GFNL AVVALVVGVLPVIPGFLEKVEIVSKVPK+F +IYG+AWFIS+FLAGFCYWGLSV 
Sbjct: 481 GFNLVAVVALVVGVLPVIPGFLEKVEIVSKVPKVFSVIYGSAWFISSFLAGFCYWGLSVL 540

Query: 541 LKRRKVSRPFGPLL 555
           LKRRK S  FGPLL
Sbjct: 541 LKRRKASLRFGPLL 554

BLAST of Lsi03G019650 vs. ExPASy TrEMBL
Match: A0A1S3B756 (purine-uracil permease NCS1 OS=Cucumis melo OX=3656 GN=LOC103486539 PE=3 SV=1)

HSP 1 Score: 1015.8 bits (2625), Expect = 7.0e-293
Identity = 512/554 (92.42%), Postives = 530/554 (95.67%), Query Frame = 0

Query: 1   MVTQSINLHLHHHIFHLPPSLSALRHRPIVSQSPLPFSPSTPKLLLKRRSNLKFTAPMAS 60
           MVT  INLHLH HIFHLPPS+S LR+RP VS SPL FSP TPKLLLKRRSNL+FT+PMAS
Sbjct: 1   MVTHCINLHLHRHIFHLPPSISTLRYRPTVSHSPLLFSPITPKLLLKRRSNLRFTSPMAS 60

Query: 61  PVDHPSEDTQNDDSGREDLRPTTPEQRTLSWLEMALLWIGLIVGVPAYYLAGSLVDLGMS 120
              +PSEDTQNDDSG++DLRPTT EQRTLSW EMALLWIGLIVGVP+YYLAGSLVDLGMS
Sbjct: 61  ADANPSEDTQNDDSGKDDLRPTTLEQRTLSWPEMALLWIGLIVGVPSYYLAGSLVDLGMS 120

Query: 121 WWQGIATVVAANAVTLIALILTGHPGTKYGISFPVFARSSFGIHGAHIPTLLRALIGCGW 180
           WWQGIATVV ANAVTLIALIL GHPGTKYGISFPVFARSSFGIHGAHIPTLLRALIGCGW
Sbjct: 121 WWQGIATVVVANAVTLIALILNGHPGTKYGISFPVFARSSFGIHGAHIPTLLRALIGCGW 180

Query: 181 YGIETWIAGEAIFLLLPESIKKSSFSQVLPWLGTSPLAFTCFLAFWVVQLASVWKGMDGI 240
           YGIETWIAGEAIFLLLPESIKKSSFSQ+LPWLGTSPLAFTCFLAFWVVQLASVWKGMDGI
Sbjct: 181 YGIETWIAGEAIFLLLPESIKKSSFSQILPWLGTSPLAFTCFLAFWVVQLASVWKGMDGI 240

Query: 241 RKLETFSAPILVFLTVCLLIWSCVRAGGFGHMLSLTSRLSSSEFWALFFPSLTANISFWA 300
           RKLETFSAPILVFLTVCLLIWSCV+AGGFGHMLSLTS+LSSSEFWALFFPSLTANISFWA
Sbjct: 241 RKLETFSAPILVFLTVCLLIWSCVKAGGFGHMLSLTSKLSSSEFWALFFPSLTANISFWA 300

Query: 301 TLALNIPDFTRYAKSQKDQIIGQLGLPIFMGAFTFVGVAVTSSTGVIFGHIISNPIELLG 360
           TLALNIPDFTRYAKSQKDQIIGQLGLPIFMGAFTFVGVAVTSSTGVIFGHIISNPI+LLG
Sbjct: 301 TLALNIPDFTRYAKSQKDQIIGQLGLPIFMGAFTFVGVAVTSSTGVIFGHIISNPIDLLG 360

Query: 361 HIGGFATKLLAIFGIILATVTTNVAANVVAPANALVNLSPSYFTFTRGALLTAVIGILFQ 420
           HIGGF TK+LAIFGIILATVTTNVAANVVAPANALVNLSPSYFTFTRGALLTAVIGILFQ
Sbjct: 361 HIGGFVTKVLAIFGIILATVTTNVAANVVAPANALVNLSPSYFTFTRGALLTAVIGILFQ 420

Query: 421 PWRLLSSSESFVYTWLVGYSALLGPIGGVMVTDYYLIRRRELSVEDLYSTSPTGAYYYSG 480
           PWRLLSSSESFVYTWLVGYSALLGPIGGVMVTDYYLIR +ELSVEDLYSTSP GAYYYSG
Sbjct: 421 PWRLLSSSESFVYTWLVGYSALLGPIGGVMVTDYYLIRGKELSVEDLYSTSPRGAYYYSG 480

Query: 481 GFNLAAVVALVVGVLPVIPGFLEKVEIVSKVPKIFGIIYGNAWFISTFLAGFCYWGLSVT 540
           GFNL AVVALVVGVLPVIPGFLEKVEIVSKVPK+F +IYG+AWFIS+FLAGFCYWGLSV 
Sbjct: 481 GFNLVAVVALVVGVLPVIPGFLEKVEIVSKVPKVFSVIYGSAWFISSFLAGFCYWGLSVL 540

Query: 541 LKRRKVSRPFGPLL 555
           LKRRK S  FGPLL
Sbjct: 541 LKRRKASLRFGPLL 554

BLAST of Lsi03G019650 vs. ExPASy TrEMBL
Match: A0A0A0LAP3 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G746620 PE=3 SV=1)

HSP 1 Score: 1001.5 bits (2588), Expect = 1.4e-288
Identity = 503/554 (90.79%), Postives = 524/554 (94.58%), Query Frame = 0

Query: 1   MVTQSINLHLHHHIFHLPPSLSALRHRPIVSQSPLPFSPSTPKLLLKRRSNLKFTAPMAS 60
           MVT  INLHLH HIFHLPPS S LR+RP  S SPLPFSP TPKLLLKRRSNL+FT+PMAS
Sbjct: 1   MVTHCINLHLHRHIFHLPPSFSTLRYRPTASPSPLPFSPITPKLLLKRRSNLQFTSPMAS 60

Query: 61  PVDHPSEDTQNDDSGREDLRPTTPEQRTLSWLEMALLWIGLIVGVPAYYLAGSLVDLGMS 120
              +P EDTQN+DSG+EDLRPTT EQRTLSW EMALLWIGLIVGVP+YYLAGSLVDLGMS
Sbjct: 61  ATANPPEDTQNEDSGKEDLRPTTLEQRTLSWPEMALLWIGLIVGVPSYYLAGSLVDLGMS 120

Query: 121 WWQGIATVVAANAVTLIALILTGHPGTKYGISFPVFARSSFGIHGAHIPTLLRALIGCGW 180
           WWQGIATVVAANAVTLIALIL GHPGTKYGISFPVFARSSFGIHGAHIPTLLRALIGCGW
Sbjct: 121 WWQGIATVVAANAVTLIALILNGHPGTKYGISFPVFARSSFGIHGAHIPTLLRALIGCGW 180

Query: 181 YGIETWIAGEAIFLLLPESIKKSSFSQVLPWLGTSPLAFTCFLAFWVVQLASVWKGMDGI 240
           YGIETWIAGEAI LLLP+SIKKS+FSQ LPWLGTSPLAFTCFL FWV+QLASVWKGMDGI
Sbjct: 181 YGIETWIAGEAISLLLPQSIKKSAFSQTLPWLGTSPLAFTCFLGFWVIQLASVWKGMDGI 240

Query: 241 RKLETFSAPILVFLTVCLLIWSCVRAGGFGHMLSLTSRLSSSEFWALFFPSLTANISFWA 300
           RKLETFSAPILVFLTVCLLIWSCV+AGGFGHMLSLTS+LS SEFWALFFPSLTANISFWA
Sbjct: 241 RKLETFSAPILVFLTVCLLIWSCVKAGGFGHMLSLTSKLSFSEFWALFFPSLTANISFWA 300

Query: 301 TLALNIPDFTRYAKSQKDQIIGQLGLPIFMGAFTFVGVAVTSSTGVIFGHIISNPIELLG 360
           TLALNIPDFTRYAKSQKDQIIGQLGLPIFMGAFTFVGVAVTSSTGVIFGHIISNPI+LL 
Sbjct: 301 TLALNIPDFTRYAKSQKDQIIGQLGLPIFMGAFTFVGVAVTSSTGVIFGHIISNPIDLLD 360

Query: 361 HIGGFATKLLAIFGIILATVTTNVAANVVAPANALVNLSPSYFTFTRGALLTAVIGILFQ 420
           HIGGFATK+LAIFGIILATVTTNVAANVVAPANALVNLSPSYFTFTRGALLTAVIGILFQ
Sbjct: 361 HIGGFATKVLAIFGIILATVTTNVAANVVAPANALVNLSPSYFTFTRGALLTAVIGILFQ 420

Query: 421 PWRLLSSSESFVYTWLVGYSALLGPIGGVMVTDYYLIRRRELSVEDLYSTSPTGAYYYSG 480
           PWRLLSSSESFVYTWLVGYSALLGPIGGVM+TDYYLIR +ELSVEDLYSTSPT AYYYSG
Sbjct: 421 PWRLLSSSESFVYTWLVGYSALLGPIGGVMMTDYYLIRGKELSVEDLYSTSPTAAYYYSG 480

Query: 481 GFNLAAVVALVVGVLPVIPGFLEKVEIVSKVPKIFGIIYGNAWFISTFLAGFCYWGLSVT 540
           GFNL AVVALVVGVLPVIPGFLEKVE+VSKVPK+F +IYG+AWF+STFLAGFCYWGLSV 
Sbjct: 481 GFNLVAVVALVVGVLPVIPGFLEKVELVSKVPKVFSVIYGSAWFVSTFLAGFCYWGLSVL 540

Query: 541 LKRRKVSRPFGPLL 555
           LKRRK S  FGP L
Sbjct: 541 LKRRKASLRFGPPL 554

BLAST of Lsi03G019650 vs. ExPASy TrEMBL
Match: A0A6J1KWR9 (purine-uracil permease NCS1-like OS=Cucurbita maxima OX=3661 GN=LOC111498292 PE=3 SV=1)

HSP 1 Score: 940.3 bits (2429), Expect = 3.8e-270
Identity = 478/551 (86.75%), Postives = 508/551 (92.20%), Query Frame = 0

Query: 6   INLHLHHHIFHLPPSLSALRHRPIVSQSPLPFSPSTPKLLLKRR--SNLKFTAPMASPVD 65
           +NLHLH H F  PP L+ +R  P VS   L FSP TPKLLL+ R  SN  F  PMAS  +
Sbjct: 18  VNLHLHRHNFRFPPCLTTVRRHPTVSL--LRFSPITPKLLLRHRFKSNTNF-IPMASAAN 77

Query: 66  HPSEDTQNDDSGREDLRPTTPEQRTLSWLEMALLWIGLIVGVPAYYLAGSLVDLGMSWWQ 125
           +P+++T  ++ G +DLRPTTPEQRTL+W EMALLWIGL+VGVPAYYLAGSLVDLGM+WWQ
Sbjct: 78  NPTDNTHVENPGSDDLRPTTPEQRTLAWPEMALLWIGLVVGVPAYYLAGSLVDLGMAWWQ 137

Query: 126 GIATVVAANAVTLIALILTGHPGTKYGISFPVFARSSFGIHGAHIPTLLRALIGCGWYGI 185
           GIATVVAANAVTLIALILTGHPGTKYGISFPVFARSSFGIHGAHIPTLLRALIGCGWYGI
Sbjct: 138 GIATVVAANAVTLIALILTGHPGTKYGISFPVFARSSFGIHGAHIPTLLRALIGCGWYGI 197

Query: 186 ETWIAGEAIFLLLPESIKKSSFSQVLPWLGTSPLAFTCFLAFWVVQLASVWKGMDGIRKL 245
           ETWIAGEAIFLLLPESIK SSFSQVLPWLGTSPLAFTCFLAFWVVQLASVWKGMDGIRKL
Sbjct: 198 ETWIAGEAIFLLLPESIKNSSFSQVLPWLGTSPLAFTCFLAFWVVQLASVWKGMDGIRKL 257

Query: 246 ETFSAPILVFLTVCLLIWSCVRAGGFGHMLSLTSRLSSSEFWALFFPSLTANISFWATLA 305
           ET SAPILVFLT+ LLIWSCVRAGGFGHMLSL S+LSSSEFWALFFPSLTANISFWATLA
Sbjct: 258 ETISAPILVFLTMGLLIWSCVRAGGFGHMLSLNSKLSSSEFWALFFPSLTANISFWATLA 317

Query: 306 LNIPDFTRYAKSQKDQIIGQLGLPIFMGAFTFVGVAVTSSTGVIFGHIISNPIELLGHIG 365
           LNIPDFTRYAKSQKDQIIGQLGLP+FMGAFTFVGVAVTSSTGVIFGHIISNPI+LLGHIG
Sbjct: 318 LNIPDFTRYAKSQKDQIIGQLGLPVFMGAFTFVGVAVTSSTGVIFGHIISNPIDLLGHIG 377

Query: 366 GFATKLLAIFGIILATVTTNVAANVVAPANALVNLSPSYFTFTRGALLTAVIGILFQPWR 425
           GFATK+LAIFGIILATVTTNVAANVVAPANALVNLSPSYFTFTRGALLTAVIGI+FQPWR
Sbjct: 378 GFATKVLAIFGIILATVTTNVAANVVAPANALVNLSPSYFTFTRGALLTAVIGIVFQPWR 437

Query: 426 LLSSSESFVYTWLVGYSALLGPIGGVMVTDYYLIRRRELSVEDLYSTSPTGAYYYSGGFN 485
           LLSSSESFVYTWLVGYSALLGPIGGVMV DYY +R+REL VEDLYS+SPTGAYYYSGGFN
Sbjct: 438 LLSSSESFVYTWLVGYSALLGPIGGVMVADYYFLRKRELIVEDLYSSSPTGAYYYSGGFN 497

Query: 486 LAAVVALVVGVLPVIPGFLEKVEIVSKVPKIFGIIYGNAWFISTFLAGFCYWGLSVTLKR 545
           L AV ALVVGVLPVIPGFLEKV+IVSKVP++F +IYGNAWFIS+F+AGFCYWGLSV LKR
Sbjct: 498 LTAVAALVVGVLPVIPGFLEKVKIVSKVPEVFTVIYGNAWFISSFIAGFCYWGLSVLLKR 557

Query: 546 RKVSRPFGPLL 555
           RKVS   GP L
Sbjct: 558 RKVSSCLGPQL 565

BLAST of Lsi03G019650 vs. ExPASy TrEMBL
Match: A0A6J1GDF7 (purine-uracil permease NCS1-like OS=Cucurbita moschata OX=3662 GN=LOC111453146 PE=3 SV=1)

HSP 1 Score: 936.8 bits (2420), Expect = 4.1e-269
Identity = 478/552 (86.59%), Postives = 508/552 (92.03%), Query Frame = 0

Query: 5   SINLHLHHHIFHLPPSLSALRHRPIVSQSPLPFSPSTPKLLLKRR--SNLKFTAPMASPV 64
           ++NLHLHHH F  P  L+ +R  P VS   L FSP TPKLLL+ R  SN  F  PMAS  
Sbjct: 17  AVNLHLHHHNFRFPACLTTVRRHPTVSL--LQFSPITPKLLLRHRFNSNTNF-IPMASAA 76

Query: 65  DHPSEDTQNDDSGREDLRPTTPEQRTLSWLEMALLWIGLIVGVPAYYLAGSLVDLGMSWW 124
           D+P+++T  ++SG +DLRPT PEQRTL+W EMALLWIGL+VGVPAYYLAGSLVDLGM+WW
Sbjct: 77  DNPTDNTHVENSGSDDLRPTMPEQRTLAWPEMALLWIGLVVGVPAYYLAGSLVDLGMAWW 136

Query: 125 QGIATVVAANAVTLIALILTGHPGTKYGISFPVFARSSFGIHGAHIPTLLRALIGCGWYG 184
           QGIATVVAANAVTLIALILTGHPGTKYGISFPVFARSSFGIHGAHIPTLLRALIGCGWYG
Sbjct: 137 QGIATVVAANAVTLIALILTGHPGTKYGISFPVFARSSFGIHGAHIPTLLRALIGCGWYG 196

Query: 185 IETWIAGEAIFLLLPESIKKSSFSQVLPWLGTSPLAFTCFLAFWVVQLASVWKGMDGIRK 244
           IETWIAGEAIFLLLPESIK SSFSQVLPWLGTSPLAFTCFLAFWVVQLASVWKGMDGIRK
Sbjct: 197 IETWIAGEAIFLLLPESIKNSSFSQVLPWLGTSPLAFTCFLAFWVVQLASVWKGMDGIRK 256

Query: 245 LETFSAPILVFLTVCLLIWSCVRAGGFGHMLSLTSRLSSSEFWALFFPSLTANISFWATL 304
           LET SAPILVFLT+ LLIWSCVRAGGFGHMLSL S+LSSSEFWALFFPSLTANISFWATL
Sbjct: 257 LETISAPILVFLTMGLLIWSCVRAGGFGHMLSLNSKLSSSEFWALFFPSLTANISFWATL 316

Query: 305 ALNIPDFTRYAKSQKDQIIGQLGLPIFMGAFTFVGVAVTSSTGVIFGHIISNPIELLGHI 364
           ALNIPDFTRYAKSQKDQIIGQLGLP+FMGAFTFVGVAVTSSTGVIFGHIISNPI+LLGHI
Sbjct: 317 ALNIPDFTRYAKSQKDQIIGQLGLPVFMGAFTFVGVAVTSSTGVIFGHIISNPIDLLGHI 376

Query: 365 GGFATKLLAIFGIILATVTTNVAANVVAPANALVNLSPSYFTFTRGALLTAVIGILFQPW 424
           GGFATK+LAIFGIILATVTTNVAANVVAPANALVNL+PSYFTFTRGALLTAVIGI+FQPW
Sbjct: 377 GGFATKVLAIFGIILATVTTNVAANVVAPANALVNLNPSYFTFTRGALLTAVIGIVFQPW 436

Query: 425 RLLSSSESFVYTWLVGYSALLGPIGGVMVTDYYLIRRRELSVEDLYSTSPTGAYYYSGGF 484
           RLLSSSESFVYTWLVGYSALLGPIGGVMV DYY IRRREL VEDLYS+SPTGAYY+SGGF
Sbjct: 437 RLLSSSESFVYTWLVGYSALLGPIGGVMVADYYFIRRRELIVEDLYSSSPTGAYYFSGGF 496

Query: 485 NLAAVVALVVGVLPVIPGFLEKVEIVSKVPKIFGIIYGNAWFISTFLAGFCYWGLSVTLK 544
           NL AV ALVVGVLPVIPGFLEKV++VSKVP++F +IYGNAWFISTF+AGFCY GLSV LK
Sbjct: 497 NLTAVAALVVGVLPVIPGFLEKVKLVSKVPQLFTVIYGNAWFISTFIAGFCYLGLSVLLK 556

Query: 545 RRKVSRPFGPLL 555
           RRKVS   GP L
Sbjct: 557 RRKVSSWLGPQL 565

BLAST of Lsi03G019650 vs. NCBI nr
Match: XP_038906223.1 (purine-uracil permease NCS1 [Benincasa hispida])

HSP 1 Score: 1043.5 bits (2697), Expect = 6.5e-301
Identity = 527/558 (94.44%), Postives = 540/558 (96.77%), Query Frame = 0

Query: 1   MVTQSINLHLHHHIFHLPPSLSALRHR----PIVSQSPLPFSPSTPKLLLKRRSNLKFTA 60
           MVTQ INLHLHHHIFHLPPSLSALRHR    P VSQSPL FSP TPKLLLKRRSNLKFT 
Sbjct: 1   MVTQCINLHLHHHIFHLPPSLSALRHRHRHHPTVSQSPLLFSPVTPKLLLKRRSNLKFTP 60

Query: 61  PMASPVDHPSEDTQNDDSGREDLRPTTPEQRTLSWLEMALLWIGLIVGVPAYYLAGSLVD 120
           PMAS VDHPSEDTQN+DSGREDLRPTTPEQRTLSW EMALLW+GLIVGVPAYYLAGSLVD
Sbjct: 61  PMASAVDHPSEDTQNEDSGREDLRPTTPEQRTLSWPEMALLWVGLIVGVPAYYLAGSLVD 120

Query: 121 LGMSWWQGIATVVAANAVTLIALILTGHPGTKYGISFPVFARSSFGIHGAHIPTLLRALI 180
           LGMSWWQGIATVVAANAVTLIALILTGHPGTKYGISFPVFARSSFGIHGAHIPTLLRALI
Sbjct: 121 LGMSWWQGIATVVAANAVTLIALILTGHPGTKYGISFPVFARSSFGIHGAHIPTLLRALI 180

Query: 181 GCGWYGIETWIAGEAIFLLLPESIKKSSFSQVLPWLGTSPLAFTCFLAFWVVQLASVWKG 240
           GCGWYGIETWIAGEAIFLLLPESIKKSSFSQVLPWLGTSPLAF CFLAFW+VQLASVWKG
Sbjct: 181 GCGWYGIETWIAGEAIFLLLPESIKKSSFSQVLPWLGTSPLAFICFLAFWIVQLASVWKG 240

Query: 241 MDGIRKLETFSAPILVFLTVCLLIWSCVRAGGFGHMLSLTSRLSSSEFWALFFPSLTANI 300
           MDGIRKLE FSAPILVFLTVCLLIWSCVRAGG GHMLSLTS+LSSSEFWALFFPSLTANI
Sbjct: 241 MDGIRKLEIFSAPILVFLTVCLLIWSCVRAGGLGHMLSLTSKLSSSEFWALFFPSLTANI 300

Query: 301 SFWATLALNIPDFTRYAKSQKDQIIGQLGLPIFMGAFTFVGVAVTSSTGVIFGHIISNPI 360
           SFWATLALNIPDFTRYAKSQKDQIIGQLGLPIFMGAFTFVGVAVTSSTG+IFGHIISNPI
Sbjct: 301 SFWATLALNIPDFTRYAKSQKDQIIGQLGLPIFMGAFTFVGVAVTSSTGLIFGHIISNPI 360

Query: 361 ELLGHIGGFATKLLAIFGIILATVTTNVAANVVAPANALVNLSPSYFTFTRGALLTAVIG 420
           ELLGHIGGFATK+LAIFGIILATVTTNVAANVVAPANALVNL+PSYFTFTRGALLTAVIG
Sbjct: 361 ELLGHIGGFATKVLAIFGIILATVTTNVAANVVAPANALVNLNPSYFTFTRGALLTAVIG 420

Query: 421 ILFQPWRLLSSSESFVYTWLVGYSALLGPIGGVMVTDYYLIRRRELSVEDLYSTSPTGAY 480
           ILFQPWRLLSSSESFVYTWLVGYSALLGPIGGVMVTDYYLIRRRELSVEDLYSTSPTGAY
Sbjct: 421 ILFQPWRLLSSSESFVYTWLVGYSALLGPIGGVMVTDYYLIRRRELSVEDLYSTSPTGAY 480

Query: 481 YYSGGFNLAAVVALVVGVLPVIPGFLEKVEIVSKVPKIFGIIYGNAWFISTFLAGFCYWG 540
           YY+GGFNLAAV ALVVGVLPVIPGFLEKV+IVSKVPK+F +IYGNAWFISTFLAGFCYWG
Sbjct: 481 YYTGGFNLAAVAALVVGVLPVIPGFLEKVKIVSKVPKVFSVIYGNAWFISTFLAGFCYWG 540

Query: 541 LSVTLKRRKVSRPFGPLL 555
           LS+ LKRRK+SR FGPLL
Sbjct: 541 LSILLKRRKLSRRFGPLL 558

BLAST of Lsi03G019650 vs. NCBI nr
Match: XP_008442760.1 (PREDICTED: purine-uracil permease NCS1 [Cucumis melo] >KAA0043951.1 purine-uracil permease NCS1 [Cucumis melo var. makuwa] >TYK25190.1 purine-uracil permease NCS1 [Cucumis melo var. makuwa])

HSP 1 Score: 1015.8 bits (2625), Expect = 1.5e-292
Identity = 512/554 (92.42%), Postives = 530/554 (95.67%), Query Frame = 0

Query: 1   MVTQSINLHLHHHIFHLPPSLSALRHRPIVSQSPLPFSPSTPKLLLKRRSNLKFTAPMAS 60
           MVT  INLHLH HIFHLPPS+S LR+RP VS SPL FSP TPKLLLKRRSNL+FT+PMAS
Sbjct: 1   MVTHCINLHLHRHIFHLPPSISTLRYRPTVSHSPLLFSPITPKLLLKRRSNLRFTSPMAS 60

Query: 61  PVDHPSEDTQNDDSGREDLRPTTPEQRTLSWLEMALLWIGLIVGVPAYYLAGSLVDLGMS 120
              +PSEDTQNDDSG++DLRPTT EQRTLSW EMALLWIGLIVGVP+YYLAGSLVDLGMS
Sbjct: 61  ADANPSEDTQNDDSGKDDLRPTTLEQRTLSWPEMALLWIGLIVGVPSYYLAGSLVDLGMS 120

Query: 121 WWQGIATVVAANAVTLIALILTGHPGTKYGISFPVFARSSFGIHGAHIPTLLRALIGCGW 180
           WWQGIATVV ANAVTLIALIL GHPGTKYGISFPVFARSSFGIHGAHIPTLLRALIGCGW
Sbjct: 121 WWQGIATVVVANAVTLIALILNGHPGTKYGISFPVFARSSFGIHGAHIPTLLRALIGCGW 180

Query: 181 YGIETWIAGEAIFLLLPESIKKSSFSQVLPWLGTSPLAFTCFLAFWVVQLASVWKGMDGI 240
           YGIETWIAGEAIFLLLPESIKKSSFSQ+LPWLGTSPLAFTCFLAFWVVQLASVWKGMDGI
Sbjct: 181 YGIETWIAGEAIFLLLPESIKKSSFSQILPWLGTSPLAFTCFLAFWVVQLASVWKGMDGI 240

Query: 241 RKLETFSAPILVFLTVCLLIWSCVRAGGFGHMLSLTSRLSSSEFWALFFPSLTANISFWA 300
           RKLETFSAPILVFLTVCLLIWSCV+AGGFGHMLSLTS+LSSSEFWALFFPSLTANISFWA
Sbjct: 241 RKLETFSAPILVFLTVCLLIWSCVKAGGFGHMLSLTSKLSSSEFWALFFPSLTANISFWA 300

Query: 301 TLALNIPDFTRYAKSQKDQIIGQLGLPIFMGAFTFVGVAVTSSTGVIFGHIISNPIELLG 360
           TLALNIPDFTRYAKSQKDQIIGQLGLPIFMGAFTFVGVAVTSSTGVIFGHIISNPI+LLG
Sbjct: 301 TLALNIPDFTRYAKSQKDQIIGQLGLPIFMGAFTFVGVAVTSSTGVIFGHIISNPIDLLG 360

Query: 361 HIGGFATKLLAIFGIILATVTTNVAANVVAPANALVNLSPSYFTFTRGALLTAVIGILFQ 420
           HIGGF TK+LAIFGIILATVTTNVAANVVAPANALVNLSPSYFTFTRGALLTAVIGILFQ
Sbjct: 361 HIGGFVTKVLAIFGIILATVTTNVAANVVAPANALVNLSPSYFTFTRGALLTAVIGILFQ 420

Query: 421 PWRLLSSSESFVYTWLVGYSALLGPIGGVMVTDYYLIRRRELSVEDLYSTSPTGAYYYSG 480
           PWRLLSSSESFVYTWLVGYSALLGPIGGVMVTDYYLIR +ELSVEDLYSTSP GAYYYSG
Sbjct: 421 PWRLLSSSESFVYTWLVGYSALLGPIGGVMVTDYYLIRGKELSVEDLYSTSPRGAYYYSG 480

Query: 481 GFNLAAVVALVVGVLPVIPGFLEKVEIVSKVPKIFGIIYGNAWFISTFLAGFCYWGLSVT 540
           GFNL AVVALVVGVLPVIPGFLEKVEIVSKVPK+F +IYG+AWFIS+FLAGFCYWGLSV 
Sbjct: 481 GFNLVAVVALVVGVLPVIPGFLEKVEIVSKVPKVFSVIYGSAWFISSFLAGFCYWGLSVL 540

Query: 541 LKRRKVSRPFGPLL 555
           LKRRK S  FGPLL
Sbjct: 541 LKRRKASLRFGPLL 554

BLAST of Lsi03G019650 vs. NCBI nr
Match: XP_004137857.1 (purine-uracil permease NCS1 [Cucumis sativus] >KGN59030.1 hypothetical protein Csa_001785 [Cucumis sativus])

HSP 1 Score: 1001.5 bits (2588), Expect = 2.8e-288
Identity = 503/554 (90.79%), Postives = 524/554 (94.58%), Query Frame = 0

Query: 1   MVTQSINLHLHHHIFHLPPSLSALRHRPIVSQSPLPFSPSTPKLLLKRRSNLKFTAPMAS 60
           MVT  INLHLH HIFHLPPS S LR+RP  S SPLPFSP TPKLLLKRRSNL+FT+PMAS
Sbjct: 1   MVTHCINLHLHRHIFHLPPSFSTLRYRPTASPSPLPFSPITPKLLLKRRSNLQFTSPMAS 60

Query: 61  PVDHPSEDTQNDDSGREDLRPTTPEQRTLSWLEMALLWIGLIVGVPAYYLAGSLVDLGMS 120
              +P EDTQN+DSG+EDLRPTT EQRTLSW EMALLWIGLIVGVP+YYLAGSLVDLGMS
Sbjct: 61  ATANPPEDTQNEDSGKEDLRPTTLEQRTLSWPEMALLWIGLIVGVPSYYLAGSLVDLGMS 120

Query: 121 WWQGIATVVAANAVTLIALILTGHPGTKYGISFPVFARSSFGIHGAHIPTLLRALIGCGW 180
           WWQGIATVVAANAVTLIALIL GHPGTKYGISFPVFARSSFGIHGAHIPTLLRALIGCGW
Sbjct: 121 WWQGIATVVAANAVTLIALILNGHPGTKYGISFPVFARSSFGIHGAHIPTLLRALIGCGW 180

Query: 181 YGIETWIAGEAIFLLLPESIKKSSFSQVLPWLGTSPLAFTCFLAFWVVQLASVWKGMDGI 240
           YGIETWIAGEAI LLLP+SIKKS+FSQ LPWLGTSPLAFTCFL FWV+QLASVWKGMDGI
Sbjct: 181 YGIETWIAGEAISLLLPQSIKKSAFSQTLPWLGTSPLAFTCFLGFWVIQLASVWKGMDGI 240

Query: 241 RKLETFSAPILVFLTVCLLIWSCVRAGGFGHMLSLTSRLSSSEFWALFFPSLTANISFWA 300
           RKLETFSAPILVFLTVCLLIWSCV+AGGFGHMLSLTS+LS SEFWALFFPSLTANISFWA
Sbjct: 241 RKLETFSAPILVFLTVCLLIWSCVKAGGFGHMLSLTSKLSFSEFWALFFPSLTANISFWA 300

Query: 301 TLALNIPDFTRYAKSQKDQIIGQLGLPIFMGAFTFVGVAVTSSTGVIFGHIISNPIELLG 360
           TLALNIPDFTRYAKSQKDQIIGQLGLPIFMGAFTFVGVAVTSSTGVIFGHIISNPI+LL 
Sbjct: 301 TLALNIPDFTRYAKSQKDQIIGQLGLPIFMGAFTFVGVAVTSSTGVIFGHIISNPIDLLD 360

Query: 361 HIGGFATKLLAIFGIILATVTTNVAANVVAPANALVNLSPSYFTFTRGALLTAVIGILFQ 420
           HIGGFATK+LAIFGIILATVTTNVAANVVAPANALVNLSPSYFTFTRGALLTAVIGILFQ
Sbjct: 361 HIGGFATKVLAIFGIILATVTTNVAANVVAPANALVNLSPSYFTFTRGALLTAVIGILFQ 420

Query: 421 PWRLLSSSESFVYTWLVGYSALLGPIGGVMVTDYYLIRRRELSVEDLYSTSPTGAYYYSG 480
           PWRLLSSSESFVYTWLVGYSALLGPIGGVM+TDYYLIR +ELSVEDLYSTSPT AYYYSG
Sbjct: 421 PWRLLSSSESFVYTWLVGYSALLGPIGGVMMTDYYLIRGKELSVEDLYSTSPTAAYYYSG 480

Query: 481 GFNLAAVVALVVGVLPVIPGFLEKVEIVSKVPKIFGIIYGNAWFISTFLAGFCYWGLSVT 540
           GFNL AVVALVVGVLPVIPGFLEKVE+VSKVPK+F +IYG+AWF+STFLAGFCYWGLSV 
Sbjct: 481 GFNLVAVVALVVGVLPVIPGFLEKVELVSKVPKVFSVIYGSAWFVSTFLAGFCYWGLSVL 540

Query: 541 LKRRKVSRPFGPLL 555
           LKRRK S  FGP L
Sbjct: 541 LKRRKASLRFGPPL 554

BLAST of Lsi03G019650 vs. NCBI nr
Match: XP_023539092.1 (purine-uracil permease NCS1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 952.2 bits (2460), Expect = 2.0e-273
Identity = 485/551 (88.02%), Postives = 512/551 (92.92%), Query Frame = 0

Query: 6   INLHLHHHIFHLPPSLSALRHRPIVSQSPLPFSPSTPKLLLKRR--SNLKFTAPMASPVD 65
           ++LHLHHH F LPP+LS +R  P VS   L FSP TPKLLL+ R  SN  FTA MAS  D
Sbjct: 18  VSLHLHHHNFRLPPALSTVRRHPTVSL--LHFSPITPKLLLRHRFKSNTNFTA-MASAAD 77

Query: 66  HPSEDTQNDDSGREDLRPTTPEQRTLSWLEMALLWIGLIVGVPAYYLAGSLVDLGMSWWQ 125
           +P+++T  ++SG +DLRPTTPEQRTL+W EMALLWIGL+VGVPAYYLAGSLVDLGM+WWQ
Sbjct: 78  NPTDNTHVENSGSDDLRPTTPEQRTLAWPEMALLWIGLVVGVPAYYLAGSLVDLGMAWWQ 137

Query: 126 GIATVVAANAVTLIALILTGHPGTKYGISFPVFARSSFGIHGAHIPTLLRALIGCGWYGI 185
           GIATVVAANAVTLIALILTGHPGTKYGISFP+FARSSFGIHGAHIPTLLRALIGCGWYGI
Sbjct: 138 GIATVVAANAVTLIALILTGHPGTKYGISFPIFARSSFGIHGAHIPTLLRALIGCGWYGI 197

Query: 186 ETWIAGEAIFLLLPESIKKSSFSQVLPWLGTSPLAFTCFLAFWVVQLASVWKGMDGIRKL 245
           ETWIAGEAIFLLLPESIK SSFSQVLPWLGTSPLAFTCFLAFWVVQLASVWKGMDGIRKL
Sbjct: 198 ETWIAGEAIFLLLPESIKNSSFSQVLPWLGTSPLAFTCFLAFWVVQLASVWKGMDGIRKL 257

Query: 246 ETFSAPILVFLTVCLLIWSCVRAGGFGHMLSLTSRLSSSEFWALFFPSLTANISFWATLA 305
           ET SAPILVFLT+ LLIWSCVRAGGFGHMLSL S+LSSSEFWALFFPSLTANISFWATLA
Sbjct: 258 ETISAPILVFLTMGLLIWSCVRAGGFGHMLSLNSKLSSSEFWALFFPSLTANISFWATLA 317

Query: 306 LNIPDFTRYAKSQKDQIIGQLGLPIFMGAFTFVGVAVTSSTGVIFGHIISNPIELLGHIG 365
           LNIPDFTRYAKSQKDQIIGQLGLP+FMGAFTFVGVAVTSSTGVIFGHIISNPI+LLGHIG
Sbjct: 318 LNIPDFTRYAKSQKDQIIGQLGLPVFMGAFTFVGVAVTSSTGVIFGHIISNPIDLLGHIG 377

Query: 366 GFATKLLAIFGIILATVTTNVAANVVAPANALVNLSPSYFTFTRGALLTAVIGILFQPWR 425
           GFATK+LAIFGIILATVTTNVAANVVAPANALVNLSPSYFTFTRGALLTAVIGI+FQPWR
Sbjct: 378 GFATKVLAIFGIILATVTTNVAANVVAPANALVNLSPSYFTFTRGALLTAVIGIVFQPWR 437

Query: 426 LLSSSESFVYTWLVGYSALLGPIGGVMVTDYYLIRRRELSVEDLYSTSPTGAYYYSGGFN 485
           LLSSSESFVYTWLVGYSALLGPIGGVMV DYY IRRREL VEDLYS SPTGAYYYSGGFN
Sbjct: 438 LLSSSESFVYTWLVGYSALLGPIGGVMVADYYFIRRRELIVEDLYSLSPTGAYYYSGGFN 497

Query: 486 LAAVVALVVGVLPVIPGFLEKVEIVSKVPKIFGIIYGNAWFISTFLAGFCYWGLSVTLKR 545
           L AV ALVVGVLPVIPGFLEKV+IVSKVP++F +IYGNAWFISTF+AGFCYWGLSV LKR
Sbjct: 498 LTAVTALVVGVLPVIPGFLEKVKIVSKVPQVFTVIYGNAWFISTFIAGFCYWGLSVLLKR 557

Query: 546 RKVSRPFGPLL 555
           RKVS   GP L
Sbjct: 558 RKVSSCLGPQL 565

BLAST of Lsi03G019650 vs. NCBI nr
Match: KAG6596517.1 (Purine-uracil permease NCS1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 944.9 bits (2441), Expect = 3.1e-271
Identity = 480/552 (86.96%), Postives = 510/552 (92.39%), Query Frame = 0

Query: 5   SINLHLHHHIFHLPPSLSALRHRPIVSQSPLPFSPSTPKLLLKR--RSNLKFTAPMASPV 64
           ++N+HLHHH F  P  L+ +R  P VS   L FSP TPKLLL+   +SN  F  PMAS  
Sbjct: 17  AVNVHLHHHNFRFPACLTTVRRHPTVSL--LQFSPITPKLLLRHGFKSNTNF-IPMASAA 76

Query: 65  DHPSEDTQNDDSGREDLRPTTPEQRTLSWLEMALLWIGLIVGVPAYYLAGSLVDLGMSWW 124
           D+P+++T  ++SG +DLRPTTPEQRTL+W EMALLWIGL+VGVPAYYLAGSLVDLGM+WW
Sbjct: 77  DNPTDNTHVENSGSDDLRPTTPEQRTLAWPEMALLWIGLVVGVPAYYLAGSLVDLGMAWW 136

Query: 125 QGIATVVAANAVTLIALILTGHPGTKYGISFPVFARSSFGIHGAHIPTLLRALIGCGWYG 184
           QGIATVVAANAVTLIALILTGHPGTKYGISFPVFARSSFGIHGAHIPTLLRALIGCGWYG
Sbjct: 137 QGIATVVAANAVTLIALILTGHPGTKYGISFPVFARSSFGIHGAHIPTLLRALIGCGWYG 196

Query: 185 IETWIAGEAIFLLLPESIKKSSFSQVLPWLGTSPLAFTCFLAFWVVQLASVWKGMDGIRK 244
           IETWIAGEAIFLLLPESIK SSFSQVLPWLGTSPLAFTCFLAFWVVQLASVWKGMDGIRK
Sbjct: 197 IETWIAGEAIFLLLPESIKNSSFSQVLPWLGTSPLAFTCFLAFWVVQLASVWKGMDGIRK 256

Query: 245 LETFSAPILVFLTVCLLIWSCVRAGGFGHMLSLTSRLSSSEFWALFFPSLTANISFWATL 304
           LET SAPILVFLT+ LLIWSCVRAGGFGHMLSL S+LSSSEFWALFFPSLTANISFWATL
Sbjct: 257 LETISAPILVFLTMGLLIWSCVRAGGFGHMLSLNSKLSSSEFWALFFPSLTANISFWATL 316

Query: 305 ALNIPDFTRYAKSQKDQIIGQLGLPIFMGAFTFVGVAVTSSTGVIFGHIISNPIELLGHI 364
           ALNIPDFTRYAKSQKDQIIGQLGLP+FMGAFTFVGVAVTSSTGVIFGHIISNPI+LLGHI
Sbjct: 317 ALNIPDFTRYAKSQKDQIIGQLGLPVFMGAFTFVGVAVTSSTGVIFGHIISNPIDLLGHI 376

Query: 365 GGFATKLLAIFGIILATVTTNVAANVVAPANALVNLSPSYFTFTRGALLTAVIGILFQPW 424
           GGFATK+LAIFGIILATVTTNVAANVVAPANALVNL+PSYFTFTRGALLTAVIGI+FQPW
Sbjct: 377 GGFATKVLAIFGIILATVTTNVAANVVAPANALVNLNPSYFTFTRGALLTAVIGIVFQPW 436

Query: 425 RLLSSSESFVYTWLVGYSALLGPIGGVMVTDYYLIRRRELSVEDLYSTSPTGAYYYSGGF 484
           RLLSSSESFVYTWLVGYSALLGPIGGVMV DYY IRRREL VEDLYS+SPTGAYYYSGGF
Sbjct: 437 RLLSSSESFVYTWLVGYSALLGPIGGVMVADYYFIRRRELIVEDLYSSSPTGAYYYSGGF 496

Query: 485 NLAAVVALVVGVLPVIPGFLEKVEIVSKVPKIFGIIYGNAWFISTFLAGFCYWGLSVTLK 544
           NL AV ALVVGVLPVIPGFLEKV+IVSKVP++F +IYGNAWFISTF+AGFCYWGLSV LK
Sbjct: 497 NLTAVAALVVGVLPVIPGFLEKVKIVSKVPQVFTVIYGNAWFISTFIAGFCYWGLSVLLK 556

Query: 545 RRKVSRPFGPLL 555
           RRKVS   GP L
Sbjct: 557 RRKVSSLLGPQL 565

BLAST of Lsi03G019650 vs. TAIR 10
Match: AT5G03555.1 (permease, cytosine/purines, uracil, thiamine, allantoin family protein )

HSP 1 Score: 716.1 bits (1847), Expect = 2.2e-206
Identity = 355/517 (68.67%), Postives = 424/517 (82.01%), Query Frame = 0

Query: 37  FSPSTP--KLLLKRRSNL--KFTAPMASPV-DHPSEDTQNDDS-GREDLRPTTPEQRTLS 96
           FSP  P   L+L R   +  + +A   S + DH  +++Q D S   +DL+PTTP QRT S
Sbjct: 74  FSPILPGDNLVLSRSGVIRPRLSAMTGSEINDHGYDESQFDPSLTNDDLKPTTPSQRTFS 133

Query: 97  WLEMALLWIGLIVGVPAYYLAGSLVDLGMSWWQGIATVVAANAVTLIALILTGHPGTKYG 156
           WL+M+ LWIGL+VGVP YYLAGSLVDLGM+WWQGIATVV AN + L+ L+LT  PGT YG
Sbjct: 134 WLDMSSLWIGLVVGVPTYYLAGSLVDLGMAWWQGIATVVTANLILLVPLVLTAQPGTLYG 193

Query: 157 ISFPVFARSSFGIHGAHIPTLLRALIGCGWYGIETWIAGEAIFLLLPESIKKSSFSQVLP 216
           ISFPV ARSSFGI GAHIPTLLRAL+GCGWYGIETWI GEAIFLLLP  IKKS+ S  LP
Sbjct: 194 ISFPVLARSSFGIRGAHIPTLLRALVGCGWYGIETWIGGEAIFLLLPGHIKKSALSHTLP 253

Query: 217 WLGTSPLAFTCFLAFWVVQLASVWKGMDGIRKLETFSAPILVFLTVCLLIWSCVRAGGFG 276
           WLGTSPL F+CF+ FW+ QL  VW+GMDGIRKLE +SAPIL+ LT CLL WS ++AGGFG
Sbjct: 254 WLGTSPLEFSCFIVFWLAQLCIVWRGMDGIRKLEKYSAPILISLTSCLLAWSYLKAGGFG 313

Query: 277 HMLSLTSRLSSSEFWALFFPSLTANISFWATLALNIPDFTRYAKSQKDQIIGQLGLPIFM 336
           HMLSL+S+L+S++FW LFFPSLTANISFWATLALNIPDF+R+AKSQ DQIIGQ+GLP+FM
Sbjct: 314 HMLSLSSKLTSAQFWTLFFPSLTANISFWATLALNIPDFSRFAKSQTDQIIGQVGLPVFM 373

Query: 337 GAFTFVGVAVTSSTGVIFGHIISNPIELLGHIGGFATKLLAIFGIILATVTTNVAANVVA 396
           G FTFVGVAVTSST +IFG +ISNPIELLG IGG AT LLAI GI LAT+TTN+AANVVA
Sbjct: 374 GLFTFVGVAVTSSTSIIFGRVISNPIELLGQIGGLATTLLAIVGISLATLTTNIAANVVA 433

Query: 397 PANALVNLSPSYFTFTRGALLTAVIGILFQPWRLLSSSESFVYTWLVGYSALLGPIGGVM 456
           PANALVNL+P +FTF RGA LTAV+GI+FQPWRLL SSESFVYTWL+GYSALLGPIGG++
Sbjct: 434 PANALVNLNPKFFTFGRGAFLTAVLGIVFQPWRLLKSSESFVYTWLIGYSALLGPIGGII 493

Query: 457 VTDYYLIRRRELSVEDLYSTSPTGAYYYSGGFNLAAVVALVVGVLPVIPGFLEKVEIVSK 516
           + DYYLI++ +L++ DLYS SP+G YY+S G+N+AAVVALV G++PV+PGFL K+  +SK
Sbjct: 494 LVDYYLIKKMKLNIGDLYSLSPSGEYYFSKGYNVAAVVALVAGIIPVVPGFLHKISALSK 553

Query: 517 VPKIFGIIYGNAWFISTFLAGFCYWGLSVTLKRRKVS 548
           +   F ++Y NA F S  +AGF YW +   L R++ S
Sbjct: 554 ISNGFVVVYDNALFFSFIIAGFVYWIIMSRLGRKQSS 590