Lsi03G019430 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi03G019430
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	Delta(14)-sterol reductase
Location	chr03: 30842899 .. 30849403 (-)
RNA-Seq Expression	Lsi03G019430
Synteny	Lsi03G019430

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATTGTTCTTAATTATTAATCAATGAACAAAAAAAGCTTTGAAACCAAATTCAGCATATTAGGAATTTGGGAAAAGCCAGCCAAGGCAGGAAGGTTCCCGCCTCGTGAAGAAATTTACAAGCTAGCAGTTCAATTCAACCAGCGCCCGCGCCCGTGGATTTAGGAGTAGGAATGAACATACTCGGGGCTCCAGTTAAAGGCTGCCAAAGTTGAAATTAGGCAATCCCGAGAAGACATTAGCCCCATTTTTGCTGCAACTCATCACTATACTAGAGGGAAAACAAGTGGGTCGAGCGACAAAATCCCAGCAAGTTATCAAGAATCGATTTTTTGAGGAATATGGATCTGGGTTTTCTTCTTCAAGCTTCAATTCCCTCTTGGGAAGCTGTAAGTGTTTGATTCCATTGCTGTGAATCACTATTTTTTTTTTATATATAAATATGATTTATACTTCACTGTGTTTGAGATTCTCCTGCTATTTGTTATTGGGCAATCAGGTTGCTATCCTCTTCATCTTCTTTTTTTACCTGGCCATTGCTGGATCTGTTCTACCTGGAAGAATTATTCCTGGAGTGATTTTGTCAGACCGTACTAGGCTTCAATATCGCTGCAATGGTCTTGTTTTCTCATACGTAAATGCAGTTTATTAAGTTTTATGCCACTGAGATTATAATTTCTTTGCAAATGAGTATTAAATTTTGGATCTCTCTTCCTCTCTGGGTTTTTTCTGCTACTGTAGGTCTACTGTCTCTTCTGGTGCTCGTTGCCCTTCTTGGGGTTGGTGTTAAGATGAATTACATTTCTCCCACTGTAAGTGTTCACTGCTTCTCATTATATATTTGATCATTCTACATATTTGGTTCCATAGACCTTTCTTTCATGGAAAATTTGTACTGATTTTCCTTCCCTGCCGTTGATAATTGTTCTTATTAGAAGCTTATAATACAACATTTATGTAGGCTATATCAGATAGAGGACTGGAGCTGCTATCAGCAACCTTCTGGTTTAGTGTCCTGGTACGTGAAACTTCAAGTTGAACATCTGGACTATTTGCTATAGAGCTGACCATTGTCCTCTTTGGCATATTATTAATATTACTCGCTGACATTTTTCTGCAATGGACTTCTGCATATCAACATCATTGTTGACTTTCATGCTTACCTTTTTCCTTTTGAGATTTGAATGGTACAAGCCAAGGGAGATTCTGAGAGCTCCTGTTGGAAGCATAACTTATACTAACCAAAAAGCTATCCCTCTAAGCCAAAGCTCTGTATTTACATACTTCCTCAATTCTCACCTATTTACTCAAGTGGTACTTCATAACCATCACATCTGCCCCAATACAACATTTCTCATTTATACTTTTTTCTAATAAAAATAATTCATATATCATTTACCGTTTCTCTCATTCTCTTTCTATGATCAAATTAATTACCAAGGGCCTACAGACACAAAATAATTATTTCTGTACCTCTGACCTTCTTGTCACTCAGTTTAAAAATAGTCAGTTGATGTTAGAATATGTTTGTTAGTTTCTTAAGGCTTCCAATTTTAATTATGAAAGGGCCTCTATAGTTCTATATGTTAAAAGAGGATTTCAATTGGAGATCTTTTCAACTTCTAACTTTGAAGGTGCGTTGTTGGGTAGAGCTCCTACACTGTCCATCAATAAGCTTGTTTGTATGCAAATCCCGAACCATCATACAAGAATTTGATGATTATTTGATTTCAATCACTAAAATGCAACACCATTACCATGAATCTCGTACTAAGAGGTTATTCATAATAACAACGTGTCCTTTTCACTGTTCGTCAAGTTTGGTCTTTGAATATTCTGGCCTTCTATTATCAATTACTAGTTTCTAACTATGTTCTCTTAACCTTCTCCTTAATATCTCACTTTCAGGCAACATTTTTACTCTGTGTTGCTGGCTACAGTTCAAGTAGTCAAAGTGCTTCTCTAAAGCCTCATATCACGGGAAATTTTATTCATGATTGGTACTTACTCAATTTTGGTTCTTTTTTTCTGTATACATATAATGTCACTAGTTCTCATTTTGTGGTGTGTTTGTTTGGCTGAGTTGTTTAAATCAAGTTAAGGAATTGAGCGCATGATGTTAAGAAATCAATTCGTTGCATATAAATGGTTTAACTTTTCTACTCATTAAAATTGTCACGTCCCATTACAGATGTAAAGAGTGATTGGTTTTGGGATCTTTACATATCACCTTTGAGAAAGAGGTGGTGACGAATGTGAATTTTTCTAGCTATTGTACATATTGCCATAACATATATGAACACCAAATCTTGCTTATATTTATATATTCTATTGGGAAGTCTATTAAATGCAATGGCTAGAATTAAATTCTGAGGCTTACTGTGTATGACTTTAGAACTTTCCATTTGTTTCTTAGCTGAATGGCTGTTAGCATTATATGGTCATGTATCACAGAATTTTCTAGTTTTATCGTTTGTTGTAAAACACATTTTGAGTTTCATAAAAAGTTGCCAATTACGGGCATATAACAACTAGAATGTAAGTACGTTGATAAATTTGTGAGAAAAGATCATTCATATTTATATTCACTCCCATCCAACCCTCCTTAGGATTTTGCTTATGTTCTTCTGAATCAGTATGGTTTAATTTGGACTTCTCTTTAAATTTGCTTATTATTTTCCAAAAAATCAACAGGATGACCATTTGGTTTTGAGTTTTTGGTTTTTGAAAATTAAGCTTACTTCCACCTATGAGTTTCTTTGTTTGATATTGACTTTCTATAAATGTTTTCAAAATCCAAGATAAATTTTAAAATTAAAAAAGGTAGTTTTTAAAAGCTTGATCTTGTTTTTGGAATTTAGCAATGAATTCAAATTTAGCCAATGCTCAATCTCTTTGGGCTCATTAGCTAAACTCTTTTGGGCTCCGTTGGGTTTGGAATCGAGGTTGCTTCTCCACCAGTGGAGGAGGTGCTTTTTTGTTTGCCATTTCGTGATAAGGGCAAAGTCTTGTGGCATGCTAACTTCTTTTGACAATGCTCAATCTCTTTGCGAGGTTTGGGAGTTGGCTAGATTTAATGCCTCCTTTTGGACATTTGTTACTAGGCCTTTTTATAATTATGATCTTGGTTTGATTTTATTGAATTAGAGCCCTTTTATGTAGTTTGTTCTAAGACTCCTTGTCAGACATGTTTTTATGCCCTTGTAATCCTTTTACTTTTTCTCAATGAAAGTTATATTTCTTCCCCCAAACATGGAAAAGTTTCTTCACAAAAATGAAAATCATTGTAAAAAAATTATGAGAAAACAAGCTTTTAAAAATAAAAAGTCAAATGGTTATCAAATGGGACTTAATTTTTTTTGTTTACTGAATTCCTGAAGTTTGATTTGACCACTTGTCTAAATGTCAGTCTTCATGTTGCTTAACAGGTGGTTTGGTATACAGTTGAATCCTCACTTTTTGGGCATTGACCTCAAGTATGTTACTTGTTTTGAACTTTTTCATGCGCCATTCAGGAATAATGTTATTAGTTTAATCACCACCTTCCATGCGACGATGCCCAAGGCCACTAATGTTTATTGATTTTCAATCCTTTGGTGCGATCCTCTTATTATCTTCACTAATAGATGGTTCTTTTCCCATGATTTCTTATCATTACTGATTAATATAAGTTCTGTTGATTCACATCTGGTGGTGTTAATATATATATTGACAAGAATGCTGAAATTCCACCTGTAGATTTTTCTTTGTGAGAGCTGGAATGATGGGCTGGCTACTTATTAACCTATCAGTTCTTGCAAGAAGTGTTTTGAATGCCACCTTGAGCCAATCGATGATCCTCTACCAGACATTTTGTGCGGTAAACTGATAATCCAAAGCATTCATTCATTTTTCTTATATTGGGGTGGCACCTAATGATTGGATGCTTGGTCTAAAATGCTGATTCAGATATATGTCTTGGACTACTTCGTTCACGAGGAGTACATGACCTCCACGTAAGTGCATTTTCAGATCTTTTGAAATGCTTCTGACCAAAGCTTCTTTGGAATGTAAGAGCAAATAGAGCTTAGATTGAAGTTTCATGCCTGATTGTCACATATTGTTGGCTCTTGATTGTATAAGAGCCAAAGTTCTTGAACGAATATAAGTCTGCCAATGGTTACTTGGAGACAGTCATGAGTCTGGCTCTTCTCTTCATAAACACGCTAATCAAAAAGTGAAATACATCATACCAAAAATGTGTTATTCCAAAAGACTAGTTTCTGTTATGCAGTTAATAATAGCTCTTCTTGAATGTTGGAAGTAGTCTCCATTCGATTTTATGCCATCGTGTGTTCTTAATTGTGTCTGCACATGAATCATGCTCATTATACATTTTAAGCATGGAACACTCTCTTCATTGCACTTTGCAATTTTGTGCAGTCAGACTGAAAATTAGTGGATGATTTATCTTTTGTAGATGGGACATAATTGCTGAGAGATTAGGCTTCATGCTGGTATTTGGGGATCTTGTTTGGATTCCTTTTACCTTTAGCATTCAGGTAAATAATGAAGTAGTTTTCTTCTTCCATCATTTCATTTTGAAATCCAAGTTTCCTATTAATGCTTAAGAGTTAAGATCTTATCTCTTTTTCCCTTTTTTTTTCCTCCCTTTTTCCTGCCGCCGCAATAACTTTGCACACAAACTGATAACTTATAAGTCACCTCAATGGCTAAATTTTTGCATGAAGTCAGAATCATTCACTTCTTTTGACTTCACTTTGTGTAAAAAATGGATGCTGAATTCTCCCATTCAAATTGAGACTCACCCTTATAATTTCGGATTGTCTTGCACATATGACTACCACAAACTCTTGAAACATTGTCTTCATTCATCAACTTATCTTTTAGAGCTTAATGCAGCTCTGTTTTTTTTTCATAATTCATTTTTTCTTCTTCTATATGCTTGCTAAGCTATATGCACACTTGTAAGCATAGCGATTTATTTATTTATTTATATTCCGGAAAATTGTCCATGCATGCCTGCAAAATCCAATGATGTACCTGAACTTTGAAAGACATGGCTTCTATAAAAAAAGTTTCTGTAAAAGATTGTCTGTGTTTGCTTTTGTTATAGGGTTGGTGGCTTCTGACAAACAAGGCGGAGCTAACAACAGCTGCTACCATAGCTAACTGCTTAGTTTTTTTAATTGGGTAGCTACCATGACATGACTTATAATTGACACTTTCTCCTCTCGGCTCGATGGTTTGCTTAATTTGAGTTTTTTGTGCATTAATTTGGGCAGATATCTTGTGTTTAGAGGATCTAACAAGCAGAAACACATGTTTAAAAAGAACAACAAAGTGCTTATATGGGGTAAGCCTCCGAAAGTTGTTGGAGGAAAGTTGCTTGCTTCTGGTTACTGGTAAGATTTTCATCCATTTAGTTTACTTGTTGCCATGGTGGACATAAAATCCAATCAGTTGAAACTTTGATTTCTCTACCAATGTTTCTTTTGAAAACCGAAAGAAAACCAAGAAATCATTAGGATGATCAGCTTCTGTCCAGCATTATATGGCAAATGTTCCCTTTTCTGTTTTTCATTTATTTTTCCTGTTTTCCATTTTCTAAGAAGGAGAAAGACTGTTTGATAGTGGTAACTGTTTCTTTTATCTCAGCTCTTGAAATTAAAAGAAACAGAATTTCTTTAACAAGTCTTTTAGAGACTTTCTTCAAGATGAAAAGACGAGAAACACAAAACAATTAGCGAATGCATACATCTCCCAAAAACTGAAAATAAGAAAGAGAATGAGAAACGGGAATGTTACCAAGACCTCATAAATTTTCAATCTTCAAAATGAGCCTCGTTGAGATCTTAAATTTGTCTAATTGTAGGCATTGATTGGACATGGAGATTTTCTAATACTTGTTCTTGAATAGGGGAATTGCTAGGCATTGTAATTACCTTGGAGACTTGTTGCTTGCACTTTCTTTCAGCTTGCCATGTGGAATAAGGTAATTATTTGGTTCCTCTGTCTTTGCAGCTAAAGAAATAAAAAACCAAACCCATGAAGAGAAATCTCCCTTCTTTTTTGCAGCTCCCCAATTCCATACTTCTATCCCATGTATCTTCTCATTCTGCTAATATGGAGAGAGAGACGAGATGAAGCTCGTTGTGCCGAGAAATATAAAGACATTTGGGCAGAGTACACCAAACTCGTTCCTTGGAGAATATTGCCATATGTTTATTAGCATGAAGATGTAGTTACGTGCGCAAAATGTGAACACTCTTGTCGCTAAGATGTTCGTCTTCTTCTTGTCTGGTACCCTCTTCTATTTTTCTTCAGGACACAATTTTCATATGTTTTCCATGTTTATTGATTCTCTGCATTTTATAAGGTCTGTGTTTAGTTGCCTTCTTGTCTGCTCATTTTGTCTCAACTTGGCCAACCTGCACTTTATTTTCTAGAACTGTTAGATTTTTTTGTGGCAATAATTATAAATATAAAAATATGCTCTCCTTTTTTT

mRNA sequence

ATTGTTCTTAATTATTAATCAATGAACAAAAAAAGCTTTGAAACCAAATTCAGCATATTAGGAATTTGGGAAAAGCCAGCCAAGGCAGGAAGGTTCCCGCCTCGTGAAGAAATTTACAAGCTAGCAGTTCAATTCAACCAGCGCCCGCGCCCGTGGATTTAGGAGTAGGAATGAACATACTCGGGGCTCCAGTTAAAGGCTGCCAAAGTTGAAATTAGGCAATCCCGAGAAGACATTAGCCCCATTTTTGCTGCAACTCATCACTATACTAGAGGGAAAACAAGTGGGTCGAGCGACAAAATCCCAGCAAGTTATCAAGAATCGATTTTTTGAGGAATATGGATCTGGGTTTTCTTCTTCAAGCTTCAATTCCCTCTTGGGAAGCTGTTGCTATCCTCTTCATCTTCTTTTTTTACCTGGCCATTGCTGGATCTGTTCTACCTGGAAGAATTATTCCTGGAGTGATTTTGTCAGACCGTACTAGGCTTCAATATCGCTGCAATGGTCTACTGTCTCTTCTGGTGCTCGTTGCCCTTCTTGGGGTTGGTGTTAAGATGAATTACATTTCTCCCACTGCTATATCAGATAGAGGACTGGAGCTGCTATCAGCAACCTTCTGGTTTAGTGTCCTGGCAACATTTTTACTCTGTGTTGCTGGCTACAGTTCAAGTAGTCAAAGTGCTTCTCTAAAGCCTCATATCACGGGAAATTTTATTCATGATTGGTGGTTTGGTATACAGTTGAATCCTCACTTTTTGGGCATTGACCTCAAATTTTTCTTTGTGAGAGCTGGAATGATGGGCTGGCTACTTATTAACCTATCAGTTCTTGCAAGAAGTGTTTTGAATGCCACCTTGAGCCAATCGATGATCCTCTACCAGACATTTTGTGCGATATATGTCTTGGACTACTTCGTTCACGAGGAGTACATGACCTCCACATGGGACATAATTGCTGAGAGATTAGGCTTCATGCTGGTATTTGGGGATCTTGTTTGGATTCCTTTTACCTTTAGCATTCAGGGTTGGTGGCTTCTGACAAACAAGGCGGAGCTAACAACAGCTGCTACCATAGCTAACTGCTTAGTTTTTTTAATTGGATATCTTGTGTTTAGAGGATCTAACAAGCAGAAACACATGTTTAAAAAGAACAACAAAGTGCTTATATGGGGTAAGCCTCCGAAAGTTGTTGGAGGAAAGTTGCTTGCTTCTGGTTACTGGGGAATTGCTAGGCATTGTAATTACCTTGGAGACTTGTTGCTTGCACTTTCTTTCAGCTTGCCATGTGGAATAAGCTCCCCAATTCCATACTTCTATCCCATGTATCTTCTCATTCTGCTAATATGGAGAGAGAGACGAGATGAAGCTCGTTGTGCCGAGAAATATAAAGACATTTGGGCAGAGTACACCAAACTCGTTCCTTGGAGAATATTGCCATATGTTTATTAGCATGAAGATGTAGTTACGTGCGCAAAATGTGAACACTCTTGTCGCTAAGATGTTCGTCTTCTTCTTGTCTGGTACCCTCTTCTATTTTTCTTCAGGACACAATTTTCATATGTTTTCCATGTTTATTGATTCTCTGCATTTTATAAGGTCTGTGTTTAGTTGCCTTCTTGTCTGCTCATTTTGTCTCAACTTGGCCAACCTGCACTTTATTTTCTAGAACTGTTAGATTTTTTTGTGGCAATAATTATAAATATAAAAATATGCTCTCCTTTTTTT

Coding sequence (CDS)

ATGGATCTGGGTTTTCTTCTTCAAGCTTCAATTCCCTCTTGGGAAGCTGTTGCTATCCTCTTCATCTTCTTTTTTTACCTGGCCATTGCTGGATCTGTTCTACCTGGAAGAATTATTCCTGGAGTGATTTTGTCAGACCGTACTAGGCTTCAATATCGCTGCAATGGTCTACTGTCTCTTCTGGTGCTCGTTGCCCTTCTTGGGGTTGGTGTTAAGATGAATTACATTTCTCCCACTGCTATATCAGATAGAGGACTGGAGCTGCTATCAGCAACCTTCTGGTTTAGTGTCCTGGCAACATTTTTACTCTGTGTTGCTGGCTACAGTTCAAGTAGTCAAAGTGCTTCTCTAAAGCCTCATATCACGGGAAATTTTATTCATGATTGGTGGTTTGGTATACAGTTGAATCCTCACTTTTTGGGCATTGACCTCAAATTTTTCTTTGTGAGAGCTGGAATGATGGGCTGGCTACTTATTAACCTATCAGTTCTTGCAAGAAGTGTTTTGAATGCCACCTTGAGCCAATCGATGATCCTCTACCAGACATTTTGTGCGATATATGTCTTGGACTACTTCGTTCACGAGGAGTACATGACCTCCACATGGGACATAATTGCTGAGAGATTAGGCTTCATGCTGGTATTTGGGGATCTTGTTTGGATTCCTTTTACCTTTAGCATTCAGGGTTGGTGGCTTCTGACAAACAAGGCGGAGCTAACAACAGCTGCTACCATAGCTAACTGCTTAGTTTTTTTAATTGGATATCTTGTGTTTAGAGGATCTAACAAGCAGAAACACATGTTTAAAAAGAACAACAAAGTGCTTATATGGGGTAAGCCTCCGAAAGTTGTTGGAGGAAAGTTGCTTGCTTCTGGTTACTGGGGAATTGCTAGGCATTGTAATTACCTTGGAGACTTGTTGCTTGCACTTTCTTTCAGCTTGCCATGTGGAATAAGCTCCCCAATTCCATACTTCTATCCCATGTATCTTCTCATTCTGCTAATATGGAGAGAGAGACGAGATGAAGCTCGTTGTGCCGAGAAATATAAAGACATTTGGGCAGAGTACACCAAACTCGTTCCTTGGAGAATATTGCCATATGTTTATTAG

Protein sequence

MDLGFLLQASIPSWEAVAILFIFFFYLAIAGSVLPGRIIPGVILSDRTRLQYRCNGLLSLLVLVALLGVGVKMNYISPTAISDRGLELLSATFWFSVLATFLLCVAGYSSSSQSASLKPHITGNFIHDWWFGIQLNPHFLGIDLKFFFVRAGMMGWLLINLSVLARSVLNATLSQSMILYQTFCAIYVLDYFVHEEYMTSTWDIIAERLGFMLVFGDLVWIPFTFSIQGWWLLTNKAELTTAATIANCLVFLIGYLVFRGSNKQKHMFKKNNKVLIWGKPPKVVGGKLLASGYWGIARHCNYLGDLLLALSFSLPCGISSPIPYFYPMYLLILLIWRERRDEARCAEKYKDIWAEYTKLVPWRILPYVY

Homology

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BLAST of Lsi03G019430 vs. ExPASy Swiss-Prot
Match: Q9LDR4 (Delta(14)-sterol reductase OS=Arabidopsis thaliana OX=3702 GN=FK PE=1 SV=2)

HSP 1 Score: 587.8 bits (1514), Expect = 8.5e-167
Identity = 278/369 (75.34%), Postives = 322/369 (87.26%), Query Frame = 0


Query: 1   MDLGFLLQASIPSWEAVAILFIFFFYLAIAGSVLPGRIIPGVILSDRTRLQYRCNGLLSL 60
           MDLG LL    PS ++V +L  +F YLA+AG +LPG++I GV+LSD ++L+YRCNGLL+L
Sbjct: 5   MDLGVLL----PSLQSVYVLVFYFVYLAVAGEILPGKVIRGVLLSDGSQLRYRCNGLLAL 64

Query: 61  LVLVALLGVGVKMNYISPTAISDRGLELLSATFWFSVLATFLLCVAGYSSSSQSASLKPH 120
           ++LVA+LG+  K+  +SP  ++DRGLELLSATF F VL T  L V G SSS++ +SLKPH
Sbjct: 65  ILLVAILGICAKLGIVSPLVVADRGLELLSATFIFCVLVTLALYVTGRSSSNKGSSLKPH 124

Query: 121 ITGNFIHDWWFGIQLNPHFLGIDLKFFFVRAGMMGWLLINLSVLARSVLNATLSQSMILY 180
           ++GN +HDWWFGIQLNP F+ IDLKFFFVRAGMMGWLLINLS+LA+SV + +LSQSMILY
Sbjct: 125 VSGNLVHDWWFGIQLNPQFMSIDLKFFFVRAGMMGWLLINLSILAKSVQDGSLSQSMILY 184

Query: 181 QTFCAIYVLDYFVHEEYMTSTWDIIAERLGFMLVFGDLVWIPFTFSIQGWWLLTNKAELT 240
           Q FCA+Y+LDYFVHEEYMTSTWDIIAERLGFMLVFGDL+WIPFTFSIQGWWLL NK ELT
Sbjct: 185 QIFCALYILDYFVHEEYMTSTWDIIAERLGFMLVFGDLLWIPFTFSIQGWWLLHNKVELT 244

Query: 241 TAATIANCLVFLIGYLVFRGSNKQKHMFKKNNKVLIWGKPPKVVGGKLLASGYWGIARHC 300
             A + NCLVFLIGY+VFRG+NKQKH+FKKN K  IWGKPP VVGGKLL SGYWGIARHC
Sbjct: 245 VPAIVVNCLVFLIGYMVFRGANKQKHIFKKNPKTPIWGKPPVVVGGKLLVSGYWGIARHC 304

Query: 301 NYLGDLLLALSFSLPCGISSPIPYFYPMYLLILLIWRERRDEARCAEKYKDIWAEYTKLV 360
           NYLGDL+LALSFSLPCGISSP+PYFYP+YLLILLIWRERRDE RCAEKYK+IWAEY +LV
Sbjct: 305 NYLGDLMLALSFSLPCGISSPVPYFYPIYLLILLIWRERRDEVRCAEKYKEIWAEYLRLV 364

Query: 361 PWRILPYVY 370
           PWRILPYVY
Sbjct: 365 PWRILPYVY 369

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BLAST of Lsi03G019430 vs. ExPASy Swiss-Prot
Match: G4SW86 (Delta(14)-sterol reductase OS=Methylomicrobium alcaliphilum (strain DSM 19304 / NCIMB 14124 / VKM B-2133 / 20Z) OX=1091494 GN=erg PE=1 SV=1)

HSP 1 Score: 269.2 bits (687), Expect = 6.7e-71
Identity = 148/364 (40.66%), Postives = 218/364 (59.89%), Query Frame = 0


Query: 12  PSWEAVAILFIFFFYLAIAGSVLPGRIIPGVILSDRTRLQYRCNGLLSLLVLVALLGVGV 71
           P+W A  +   +F   A      PG  + G+ L D +RL YR NG+ S L  +A++   V
Sbjct: 69  PTWHAAGLYAAWFLGQAALQVWAPGPTVQGMKLPDGSRLDYRMNGIFSFLFTLAVVFGLV 128

Query: 72  KMNYISPTAISDRGLELLSATFWFSVLATFLLCVAGYSSSSQSASLKPHITGNFIHDWWF 131
            M ++  T + D+   LL+    F+ +    L   G +        +P  TG   +D++ 
Sbjct: 129 TMGWLDATVLYDQLGPLLTVVNIFTFVFAGFLYFWGLNGKQWE---RP--TGRPFYDYFM 188

Query: 132 GIQLNPHFLGIDLKFFF-VRAGMMGWLLINLSVLARSV-LNATLSQSMILYQTFCAIYVL 191
           G  LNP    +DLK F   R GM+ WLL+NLS+ A+   L+ T++  M+L   F + Y++
Sbjct: 189 GTALNPRIGSLDLKLFCEARPGMIFWLLMNLSMAAKQYELHGTVTVPMLLVVGFQSFYLI 248

Query: 192 DYFVHEEYMTSTWDIIAERLGFMLVFGDLVWIPFTFSIQGWWLLTNKAELTTAATIANCL 251
           DYF+HEE + +TWDI  E+ G+ML +GDLVW+PFT+++Q  +L+ +  +L     IA   
Sbjct: 249 DYFIHEEAVLTTWDIKHEKFGWMLCWGDLVWLPFTYTLQAQYLVHHTHDLPVWGIIAIVA 308

Query: 252 VFLIGYLVFRGSNKQKHMFKKNNKVLIWGKPPKVV----GGKLLASGYWGIARHCNYLGD 311
           + L GY +FRG+N QKH F+++   ++WGKP K +    G  LL SG+WGIARH NY GD
Sbjct: 309 LNLAGYAIFRGANIQKHHFRRDPNRIVWGKPAKYIKTKQGSLLLTSGWWGIARHMNYFGD 368

Query: 312 LLLALSFSLPCGISSPIPYFYPMYLLILLIWRERRDEARCAEKYKDIWAEYTKLVPWRIL 370
           L++ALS+ LP    SPIPYF+ +Y  ILL+ RE+RD+A C  KY + W +Y K VPWRI+
Sbjct: 369 LMIALSWCLPAAFGSPIPYFHIVYFTILLLHREKRDDAMCLAKYGEDWLQYRKKVPWRIV 427

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BLAST of Lsi03G019430 vs. ExPASy Swiss-Prot
Match: Q71KT5 (Delta(14)-sterol reductase TM7SF2 OS=Mus musculus OX=10090 GN=Tm7sf2 PE=1 SV=2)

HSP 1 Score: 247.3 bits (630), Expect = 2.7e-64
Identity = 148/363 (40.77%), Postives = 218/363 (60.06%), Query Frame = 0


Query: 14  WEAVAILFIFFFY-LAIAGSVLPGR-IIPGVILSDRTRLQYRCNGLLSLLVLVALLGVGV 73
           W   A+L +F +  L +A  +LP R +  G+ L D++RL+Y  NG  +L++   L+G+GV
Sbjct: 58  WSPWALLLLFIWLGLQVALYLLPARKVAEGLELKDKSRLRYPINGFQALVLTALLMGLGV 117

Query: 74  KMNYISPTAISDRGLELLSATFWFSVLATFLLCVAGYSSSSQSASLKPHITGNFIHDWWF 133
            +  +   A+    L L  AT   S + + LL      + + SA      +GN ++D++ 
Sbjct: 118 SVG-LPLGALPGMLLPLAFATTLTSFIFSLLLYAKALVAPA-SALAPGGNSGNSMYDFFL 177

Query: 134 GIQLNPHFLGIDLKFFF-VRAGMMGWLLINLSVLARSV-LNATLSQSMILYQTFCAIYVL 193
           G +LNP     D K+F  +R G++GW+ INL++L +   L  + S +M L   F  +YV 
Sbjct: 178 GRELNPRLGSFDFKYFCELRPGLIGWVFINLALLMQEAELRGSPSLAMWLVNGFQLLYVG 237

Query: 194 DYFVHEEYMTSTWDIIAERLGFMLVFGDLVWIPFTFSIQGWWLLTNKAELTTAATIANCL 253
           D   +EE + +T DII +  GFMLVFGDL W+PFT+S+Q  +LL +   L     +  CL
Sbjct: 238 DALWYEESVLTTMDIIHDGFGFMLVFGDLAWVPFTYSLQAQFLLYHPQPLGLPMALLICL 297

Query: 254 VFLIGYLVFRGSNKQKHMFKKN---NKVLIWGKPPKVVGGKLLASGYWGIARHCNYLGDL 313
           + +IGY +FRG+N QK+ F+KN     V      P   G +LL SG+WG+ RH NYLGDL
Sbjct: 298 LKVIGYYIFRGANSQKNTFRKNPSDPSVAGLETIPTATGRQLLVSGWWGMVRHPNYLGDL 357

Query: 314 LLALSFSLPCGISSPIPYFYPMYLLILLIWRERRDEARCAEKYKDIWAEYTKLVPWRILP 370
           ++AL++SLPCG+S  +PYFY +Y   LL+ RE RDE +C +KY   W EY K VP+RI+P
Sbjct: 358 IMALAWSLPCGLSHLLPYFYVLYFTALLVHREARDEQQCLQKYGRAWQEYCKRVPYRIIP 417

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BLAST of Lsi03G019430 vs. ExPASy Swiss-Prot
Match: O08984 (Delta(14)-sterol reductase LBR OS=Rattus norvegicus OX=10116 GN=Lbr PE=1 SV=1)

HSP 1 Score: 236.5 bits (602), Expect = 4.8e-61
Identity = 143/365 (39.18%), Postives = 212/365 (58.08%), Query Frame = 0


Query: 14  WEA-VAILFIFFFYLAIAGSVLP-GRIIPGVILSDRTRLQYRCNGLLSLLVLVALLGVGV 73
           WEA V  +++ +F+L    S+LP G+++ G  L D  RL+YR NGL + ++  A +G  V
Sbjct: 259 WEARVCGVYLLWFFLQALFSLLPVGKVVEGTPLVDGRRLKYRLNGLYAFILTSAAVGTAV 318

Query: 74  KMNYISPTAISDRGLELLSATFWFSVLATFLLCVAGYSSSSQSASLKPHITGNFIHDWWF 133
             + I    +    L+   A   FSV+ +  L     S       L P  +GN ++D++ 
Sbjct: 319 FWD-IELYYLYTHFLQFALAAIVFSVVLSVYLYAR--SLKVPRDELSPASSGNAVYDFFI 378

Query: 134 GIQLNPHFLGIDLKFFF-VRAGMMGWLLINLSVLARSVL---NATLSQSMILYQTFCAIY 193
           G +LNP     DLKFF  +R G++GW++INL +L   +     +  S +M L  +F  +Y
Sbjct: 379 GRELNPRIGAFDLKFFCELRPGLIGWVVINLVMLLAEMKVQERSAPSLAMTLVNSFQLLY 438

Query: 194 VLDYFVHEEYMTSTWDIIAERLGFMLVFGDLVWIPFTFSIQGWWLLTNKAELTTAATIAN 253
           V+D    EE + +T DII +  GFML FGDLVW+PFT+S+Q ++L+ +  +L+   T   
Sbjct: 439 VVDALWFEEALLTTMDIIHDGFGFMLAFGDLVWVPFTYSLQAFYLVNHPQDLSWPLTSVI 498

Query: 254 CLVFLIGYLVFRGSNKQKHMFKKN---NKVLIWGKPPKVVGGKLLASGYWGIARHCNYLG 313
             + L GY++FR +N QK+ F+KN    K+      P      LL SG+WG  RH NYLG
Sbjct: 499 IALKLCGYVIFRCANSQKNAFRKNPTDPKLAHLKTIPTSTWKSLLVSGWWGFVRHPNYLG 558

Query: 314 DLLLALSFSLPCGISSPIPYFYPMYLLILLIWRERRDEARCAEKYKDIWAEYTKLVPWRI 370
           DL++AL++SLPCG +  +PYFY +Y   LLI RE RDE +C  KY   W +Y + VP+RI
Sbjct: 559 DLIMALAWSLPCGFNHILPYFYVIYFTALLIHREARDEHQCRRKYGLAWEKYCQRVPYRI 618

[Close]

BLAST of Lsi03G019430 vs. ExPASy Swiss-Prot
Match: Q3U9G9 (Delta(14)-sterol reductase LBR OS=Mus musculus OX=10090 GN=Lbr PE=1 SV=2)

HSP 1 Score: 235.7 bits (600), Expect = 8.2e-61
Identity = 145/369 (39.30%), Postives = 216/369 (58.54%), Query Frame = 0


Query: 14  WE-AVAILFIFFFYLAIAGSVLP-GRIIPGVILSDRTRLQYRCNGLLSLLVLVALLGV-- 73
           WE  V  +++ +F++     +LP G++  G  L D  RLQYR NGL + ++  A LG   
Sbjct: 265 WEPRVCGVYLLWFFVQALFHLLPVGKVAEGTPLVDGRRLQYRLNGLYAFILTSAALGAAV 324

Query: 74  --GVKMNYISPTAISDRGLELLSATFWFSVLATFLLCVAGYSSSSQSASLKPHITGNFIH 133
             GV++ Y     +    L+L  A   FSVL +  L V   S  +    L P  +GN ++
Sbjct: 325 FWGVELCY-----LYTHFLQLALAATGFSVLLSAYLYVR--SLRAPREELSPASSGNAVY 384

Query: 134 DWWFGIQLNPHFLGIDLKFFF-VRAGMMGWLLINLSVLARSVL---NATLSQSMILYQTF 193
           D++ G +LNP     DLKFF  +R G++GW++INL +L   +     A  S +MIL  +F
Sbjct: 385 DFFIGRELNPRLGAFDLKFFCELRPGLIGWVVINLVMLLMEMKIQERAAPSLAMILVNSF 444

Query: 194 CAIYVLDYFVHEEYMTSTWDIIAERLGFMLVFGDLVWIPFTFSIQGWWLLTNKAELTTAA 253
             +YV+D   +EE + ++ DI+ +  GFML FGDLVW+PFT+S+Q ++L+++  +L+   
Sbjct: 445 QLLYVVDALWNEEALLTSMDIMHDGFGFMLAFGDLVWVPFTYSLQAFYLVSHPHDLSWPL 504

Query: 254 TIANCLVFLIGYLVFRGSNKQKHMFKKN---NKVLIWGKPPKVVGGKLLASGYWGIARHC 313
                 + L GY++FR +N QK+ F+KN    K+          G  LL SG+WG  RH 
Sbjct: 505 ASVIIALKLCGYVIFRCANSQKNAFRKNPTDPKLAHLKTIHTSTGKSLLVSGWWGFVRHP 564

Query: 314 NYLGDLLLALSFSLPCGISSPIPYFYPMYLLILLIWRERRDEARCAEKYKDIWAEYTKLV 370
           NYLGDL++AL++SLPCG +  +PYFY +Y   LLI RE RDE +C  KY   W +Y + V
Sbjct: 565 NYLGDLIMALAWSLPCGFNHLLPYFYIIYFTALLIHREARDEHQCRRKYGLAWEKYCQRV 624

[Close]

BLAST of Lsi03G019430 vs. ExPASy TrEMBL
Match: A0A5A7TKR7 (Delta(14)-sterol reductase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G003450 PE=3 SV=1)

HSP 1 Score: 743.0 bits (1917), Expect = 5.9e-211
Identity = 365/369 (98.92%), Postives = 367/369 (99.46%), Query Frame = 0


Query: 1   MDLGFLLQASIPSWEAVAILFIFFFYLAIAGSVLPGRIIPGVILSDRTRLQYRCNGLLSL 60
           MDLGFL QASIPSWEAVAILFIFFFYLAIAGSVLPGR+IPGVILSDRTRLQYRCNGLLSL
Sbjct: 1   MDLGFLFQASIPSWEAVAILFIFFFYLAIAGSVLPGRVIPGVILSDRTRLQYRCNGLLSL 60

Query: 61  LVLVALLGVGVKMNYISPTAISDRGLELLSATFWFSVLATFLLCVAGYSSSSQSASLKPH 120
           LVLVALLGVGVKMNYISPTAISDRGLELLSATFWFSVLATFLLCVAGYSSSSQSASLKPH
Sbjct: 61  LVLVALLGVGVKMNYISPTAISDRGLELLSATFWFSVLATFLLCVAGYSSSSQSASLKPH 120

Query: 121 ITGNFIHDWWFGIQLNPHFLGIDLKFFFVRAGMMGWLLINLSVLARSVLNATLSQSMILY 180
           ITGNFIHDWWFGIQLNPHFLGIDLKFFFVRAGMMGWLLINLSVLARSVLNATLSQSMILY
Sbjct: 121 ITGNFIHDWWFGIQLNPHFLGIDLKFFFVRAGMMGWLLINLSVLARSVLNATLSQSMILY 180

Query: 181 QTFCAIYVLDYFVHEEYMTSTWDIIAERLGFMLVFGDLVWIPFTFSIQGWWLLTNKAELT 240
           QTFCAIYVLDYFVHEEYMTSTWDIIAERLGFMLVFGDLVWIPFTFSIQGWWLLTNKAELT
Sbjct: 181 QTFCAIYVLDYFVHEEYMTSTWDIIAERLGFMLVFGDLVWIPFTFSIQGWWLLTNKAELT 240

Query: 241 TAATIANCLVFLIGYLVFRGSNKQKHMFKKNNKVLIWGKPPKVVGGKLLASGYWGIARHC 300
           TAATIANCL+FLIGYLVFRGSNKQKHMFKKNNK LIWGKPPKVVGGKLLASGYWGIARHC
Sbjct: 241 TAATIANCLIFLIGYLVFRGSNKQKHMFKKNNKALIWGKPPKVVGGKLLASGYWGIARHC 300

Query: 301 NYLGDLLLALSFSLPCGISSPIPYFYPMYLLILLIWRERRDEARCAEKYKDIWAEYTKLV 360
           NYLGDLLLALSFSLPCGISSPIPYFYPMYLLILLIWRERRDEARCAEKYKDIWAEYTKLV
Sbjct: 301 NYLGDLLLALSFSLPCGISSPIPYFYPMYLLILLIWRERRDEARCAEKYKDIWAEYTKLV 360

Query: 361 PWRILPYVY 370
           PWRILPYVY
Sbjct: 361 PWRILPYVY 369

[Close]

BLAST of Lsi03G019430 vs. ExPASy TrEMBL
Match: A0A1S3B6J9 (LOW QUALITY PROTEIN: delta(14)-sterol reductase OS=Cucumis melo OX=3656 GN=LOC103486569 PE=3 SV=1)

HSP 1 Score: 740.3 bits (1910), Expect = 3.8e-210
Identity = 363/369 (98.37%), Postives = 366/369 (99.19%), Query Frame = 0


Query: 1   MDLGFLLQASIPSWEAVAILFIFFFYLAIAGSVLPGRIIPGVILSDRTRLQYRCNGLLSL 60
           MDLGFL QASIPSWEAVAILFIFFFYLAIAGSVLPGR+IPGVILSDRTRLQYRCNGLLSL
Sbjct: 1   MDLGFLFQASIPSWEAVAILFIFFFYLAIAGSVLPGRVIPGVILSDRTRLQYRCNGLLSL 60

Query: 61  LVLVALLGVGVKMNYISPTAISDRGLELLSATFWFSVLATFLLCVAGYSSSSQSASLKPH 120
           LVLVALLGVGVKMNYISPTAISDRGLELLSATFWFSVLATFLLCVAGYSSSSQSASLKPH
Sbjct: 61  LVLVALLGVGVKMNYISPTAISDRGLELLSATFWFSVLATFLLCVAGYSSSSQSASLKPH 120

Query: 121 ITGNFIHDWWFGIQLNPHFLGIDLKFFFVRAGMMGWLLINLSVLARSVLNATLSQSMILY 180
           ITGNFIHDWWFGIQLNPHFLGIDLKFFFVRAGMMGWLLINLSVLARSVLNATLSQSMILY
Sbjct: 121 ITGNFIHDWWFGIQLNPHFLGIDLKFFFVRAGMMGWLLINLSVLARSVLNATLSQSMILY 180

Query: 181 QTFCAIYVLDYFVHEEYMTSTWDIIAERLGFMLVFGDLVWIPFTFSIQGWWLLTNKAELT 240
           QTFCAIYVLDYFVHEEYMTSTWDIIAERLGFMLVFGDLVWIPFTFSIQGWWLLTNKAELT
Sbjct: 181 QTFCAIYVLDYFVHEEYMTSTWDIIAERLGFMLVFGDLVWIPFTFSIQGWWLLTNKAELT 240

Query: 241 TAATIANCLVFLIGYLVFRGSNKQKHMFKKNNKVLIWGKPPKVVGGKLLASGYWGIARHC 300
           TAATIANCL+F +GYLVFRGSNKQKHMFKKNNK LIWGKPPKVVGGKLLASGYWGIARHC
Sbjct: 241 TAATIANCLIFXLGYLVFRGSNKQKHMFKKNNKALIWGKPPKVVGGKLLASGYWGIARHC 300

Query: 301 NYLGDLLLALSFSLPCGISSPIPYFYPMYLLILLIWRERRDEARCAEKYKDIWAEYTKLV 360
           NYLGDLLLALSFSLPCGISSPIPYFYPMYLLILLIWRERRDEARCAEKYKDIWAEYTKLV
Sbjct: 301 NYLGDLLLALSFSLPCGISSPIPYFYPMYLLILLIWRERRDEARCAEKYKDIWAEYTKLV 360

Query: 361 PWRILPYVY 370
           PWRILPYVY
Sbjct: 361 PWRILPYVY 369

[Close]

BLAST of Lsi03G019430 vs. ExPASy TrEMBL
Match: A0A0A0LB94 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G748820 PE=3 SV=1)

HSP 1 Score: 736.1 bits (1899), Expect = 7.2e-209
Identity = 361/369 (97.83%), Postives = 366/369 (99.19%), Query Frame = 0


Query: 1   MDLGFLLQASIPSWEAVAILFIFFFYLAIAGSVLPGRIIPGVILSDRTRLQYRCNGLLSL 60
           MDLGFLLQASIPSWEAVAILFIFFFYLAIAGSVLPGR+IPGVILSDR+RLQYRCNGLLSL
Sbjct: 1   MDLGFLLQASIPSWEAVAILFIFFFYLAIAGSVLPGRVIPGVILSDRSRLQYRCNGLLSL 60

Query: 61  LVLVALLGVGVKMNYISPTAISDRGLELLSATFWFSVLATFLLCVAGYSSSSQSASLKPH 120
           LVLVALLGVGVKMNYISPTAI+DRGLELLSATFWFSVLATFLLCVAGYSSSSQSASLKPH
Sbjct: 61  LVLVALLGVGVKMNYISPTAIADRGLELLSATFWFSVLATFLLCVAGYSSSSQSASLKPH 120

Query: 121 ITGNFIHDWWFGIQLNPHFLGIDLKFFFVRAGMMGWLLINLSVLARSVLNATLSQSMILY 180
           IT NFIHDWWFG+QLNPHFLGIDLKFFFVRAGMMGWLLINLSVLARSVLNATLSQSMILY
Sbjct: 121 ITRNFIHDWWFGMQLNPHFLGIDLKFFFVRAGMMGWLLINLSVLARSVLNATLSQSMILY 180

Query: 181 QTFCAIYVLDYFVHEEYMTSTWDIIAERLGFMLVFGDLVWIPFTFSIQGWWLLTNKAELT 240
           QTFCAIYVLDYFVHEEYMTSTWDIIAERLGFMLVFGDLVWIPFTFSIQGWWLLTNK ELT
Sbjct: 181 QTFCAIYVLDYFVHEEYMTSTWDIIAERLGFMLVFGDLVWIPFTFSIQGWWLLTNKVELT 240

Query: 241 TAATIANCLVFLIGYLVFRGSNKQKHMFKKNNKVLIWGKPPKVVGGKLLASGYWGIARHC 300
           TAATIANCLVFLIGYLVFRGSNKQKHMFKKNNK LIWGKPPKVVGGKLLASGYWGIARHC
Sbjct: 241 TAATIANCLVFLIGYLVFRGSNKQKHMFKKNNKALIWGKPPKVVGGKLLASGYWGIARHC 300

Query: 301 NYLGDLLLALSFSLPCGISSPIPYFYPMYLLILLIWRERRDEARCAEKYKDIWAEYTKLV 360
           NYLGDLLLALSFSLPCGISSP+PYFYPMYLLILLIWRERRDEARCAEKYKDIWAEYTKLV
Sbjct: 301 NYLGDLLLALSFSLPCGISSPVPYFYPMYLLILLIWRERRDEARCAEKYKDIWAEYTKLV 360

Query: 361 PWRILPYVY 370
           PWRILPYVY
Sbjct: 361 PWRILPYVY 369

[Close]

BLAST of Lsi03G019430 vs. ExPASy TrEMBL
Match: A0A6J1F6L0 (delta(14)-sterol reductase-like OS=Cucurbita moschata OX=3662 GN=LOC111441342 PE=3 SV=1)

HSP 1 Score: 721.8 bits (1862), Expect = 1.4e-204
Identity = 354/369 (95.93%), Postives = 361/369 (97.83%), Query Frame = 0


Query: 1   MDLGFLLQASIPSWEAVAILFIFFFYLAIAGSVLPGRIIPGVILSDRTRLQYRCNGLLSL 60
           MDL  LLQ  IPSWEAVAILFIFFFYLAIAGSVLPGRIIPGVILSDRTRL YRCNGLLSL
Sbjct: 1   MDLDSLLQVLIPSWEAVAILFIFFFYLAIAGSVLPGRIIPGVILSDRTRLHYRCNGLLSL 60

Query: 61  LVLVALLGVGVKMNYISPTAISDRGLELLSATFWFSVLATFLLCVAGYSSSSQSASLKPH 120
           L+LVALLG+GVKMNYISPTAISDRGLELLSATFWFSVLATFLLC+AGYSSSSQS+SLKP 
Sbjct: 61  LLLVALLGIGVKMNYISPTAISDRGLELLSATFWFSVLATFLLCIAGYSSSSQSSSLKPR 120

Query: 121 ITGNFIHDWWFGIQLNPHFLGIDLKFFFVRAGMMGWLLINLSVLARSVLNATLSQSMILY 180
            TGNFIHDWWFGIQLNPHFLGIDLKFFFVRAGMMGWLLINLSVLARSVLNATLSQSMILY
Sbjct: 121 ATGNFIHDWWFGIQLNPHFLGIDLKFFFVRAGMMGWLLINLSVLARSVLNATLSQSMILY 180

Query: 181 QTFCAIYVLDYFVHEEYMTSTWDIIAERLGFMLVFGDLVWIPFTFSIQGWWLLTNKAELT 240
           QTFCAIYV+DYFVHEEYMTSTWDIIAERLGFMLVFGDLVWIPFTFSIQGWWLLTN+AELT
Sbjct: 181 QTFCAIYVIDYFVHEEYMTSTWDIIAERLGFMLVFGDLVWIPFTFSIQGWWLLTNRAELT 240

Query: 241 TAATIANCLVFLIGYLVFRGSNKQKHMFKKNNKVLIWGKPPKVVGGKLLASGYWGIARHC 300
           TAATIANCLVFLIGYLVFRGSNKQKHMFKKN+K LIWGKPPKVVGGKLLASGYWGIARHC
Sbjct: 241 TAATIANCLVFLIGYLVFRGSNKQKHMFKKNSKALIWGKPPKVVGGKLLASGYWGIARHC 300

Query: 301 NYLGDLLLALSFSLPCGISSPIPYFYPMYLLILLIWRERRDEARCAEKYKDIWAEYTKLV 360
           NYLGDLLLALSFSLPCGISSPIPYFYPMYLLILLIWRERRDEARCAEKYKDIWAEYTKLV
Sbjct: 301 NYLGDLLLALSFSLPCGISSPIPYFYPMYLLILLIWRERRDEARCAEKYKDIWAEYTKLV 360

Query: 361 PWRILPYVY 370
           PWRILPYVY
Sbjct: 361 PWRILPYVY 369

[Close]

BLAST of Lsi03G019430 vs. ExPASy TrEMBL
Match: A0A6J1J0W9 (delta(14)-sterol reductase-like OS=Cucurbita maxima OX=3661 GN=LOC111481674 PE=3 SV=1)

HSP 1 Score: 719.2 bits (1855), Expect = 9.1e-204
Identity = 351/369 (95.12%), Postives = 361/369 (97.83%), Query Frame = 0


Query: 1   MDLGFLLQASIPSWEAVAILFIFFFYLAIAGSVLPGRIIPGVILSDRTRLQYRCNGLLSL 60
           MDL  LLQ  IPSWEAVAILFIFFFYLAIAGSVLPGRIIPGVILSDRTRL YRCNGLLSL
Sbjct: 1   MDLDSLLQVPIPSWEAVAILFIFFFYLAIAGSVLPGRIIPGVILSDRTRLHYRCNGLLSL 60

Query: 61  LVLVALLGVGVKMNYISPTAISDRGLELLSATFWFSVLATFLLCVAGYSSSSQSASLKPH 120
           L+LVALLG+GVKMNYISPTAISDRGLELLSATFWFSVLATFLLC+AGYSSSSQS+SLKP 
Sbjct: 61  LLLVALLGIGVKMNYISPTAISDRGLELLSATFWFSVLATFLLCIAGYSSSSQSSSLKPR 120

Query: 121 ITGNFIHDWWFGIQLNPHFLGIDLKFFFVRAGMMGWLLINLSVLARSVLNATLSQSMILY 180
            TGNFIHDWWFGIQLNPHFLGIDLKFFFVRAGMMGWLLINLSVLARSVLNATLSQSMILY
Sbjct: 121 ATGNFIHDWWFGIQLNPHFLGIDLKFFFVRAGMMGWLLINLSVLARSVLNATLSQSMILY 180

Query: 181 QTFCAIYVLDYFVHEEYMTSTWDIIAERLGFMLVFGDLVWIPFTFSIQGWWLLTNKAELT 240
           QTFCAIYV+DYF+HEEYMTSTWDIIAERLGFMLVFGDLVWIPFTFSIQGWWL+TN+AELT
Sbjct: 181 QTFCAIYVIDYFIHEEYMTSTWDIIAERLGFMLVFGDLVWIPFTFSIQGWWLMTNRAELT 240

Query: 241 TAATIANCLVFLIGYLVFRGSNKQKHMFKKNNKVLIWGKPPKVVGGKLLASGYWGIARHC 300
           TAATIANCLVFLIGYLVFRGSNKQKHMFKK++K LIWGKPPKVVGGKLLASGYWGIARHC
Sbjct: 241 TAATIANCLVFLIGYLVFRGSNKQKHMFKKSSKALIWGKPPKVVGGKLLASGYWGIARHC 300

Query: 301 NYLGDLLLALSFSLPCGISSPIPYFYPMYLLILLIWRERRDEARCAEKYKDIWAEYTKLV 360
           NYLGDLLLALSFSLPCGISSPIPYFYPMYLLILLIWRERRDEARCAEKYKDIWAEYTKLV
Sbjct: 301 NYLGDLLLALSFSLPCGISSPIPYFYPMYLLILLIWRERRDEARCAEKYKDIWAEYTKLV 360

Query: 361 PWRILPYVY 370
           PWRILPYVY
Sbjct: 361 PWRILPYVY 369

[Close]

BLAST of Lsi03G019430 vs. NCBI nr
Match: KAA0043924.1 (delta(14)-sterol reductase [Cucumis melo var. makuwa] >TYK25215.1 delta(14)-sterol reductase [Cucumis melo var. makuwa])

HSP 1 Score: 743.0 bits (1917), Expect = 1.2e-210
Identity = 365/369 (98.92%), Postives = 367/369 (99.46%), Query Frame = 0


Query: 1   MDLGFLLQASIPSWEAVAILFIFFFYLAIAGSVLPGRIIPGVILSDRTRLQYRCNGLLSL 60
           MDLGFL QASIPSWEAVAILFIFFFYLAIAGSVLPGR+IPGVILSDRTRLQYRCNGLLSL
Sbjct: 1   MDLGFLFQASIPSWEAVAILFIFFFYLAIAGSVLPGRVIPGVILSDRTRLQYRCNGLLSL 60

Query: 61  LVLVALLGVGVKMNYISPTAISDRGLELLSATFWFSVLATFLLCVAGYSSSSQSASLKPH 120
           LVLVALLGVGVKMNYISPTAISDRGLELLSATFWFSVLATFLLCVAGYSSSSQSASLKPH
Sbjct: 61  LVLVALLGVGVKMNYISPTAISDRGLELLSATFWFSVLATFLLCVAGYSSSSQSASLKPH 120

Query: 121 ITGNFIHDWWFGIQLNPHFLGIDLKFFFVRAGMMGWLLINLSVLARSVLNATLSQSMILY 180
           ITGNFIHDWWFGIQLNPHFLGIDLKFFFVRAGMMGWLLINLSVLARSVLNATLSQSMILY
Sbjct: 121 ITGNFIHDWWFGIQLNPHFLGIDLKFFFVRAGMMGWLLINLSVLARSVLNATLSQSMILY 180

Query: 181 QTFCAIYVLDYFVHEEYMTSTWDIIAERLGFMLVFGDLVWIPFTFSIQGWWLLTNKAELT 240
           QTFCAIYVLDYFVHEEYMTSTWDIIAERLGFMLVFGDLVWIPFTFSIQGWWLLTNKAELT
Sbjct: 181 QTFCAIYVLDYFVHEEYMTSTWDIIAERLGFMLVFGDLVWIPFTFSIQGWWLLTNKAELT 240

Query: 241 TAATIANCLVFLIGYLVFRGSNKQKHMFKKNNKVLIWGKPPKVVGGKLLASGYWGIARHC 300
           TAATIANCL+FLIGYLVFRGSNKQKHMFKKNNK LIWGKPPKVVGGKLLASGYWGIARHC
Sbjct: 241 TAATIANCLIFLIGYLVFRGSNKQKHMFKKNNKALIWGKPPKVVGGKLLASGYWGIARHC 300

Query: 301 NYLGDLLLALSFSLPCGISSPIPYFYPMYLLILLIWRERRDEARCAEKYKDIWAEYTKLV 360
           NYLGDLLLALSFSLPCGISSPIPYFYPMYLLILLIWRERRDEARCAEKYKDIWAEYTKLV
Sbjct: 301 NYLGDLLLALSFSLPCGISSPIPYFYPMYLLILLIWRERRDEARCAEKYKDIWAEYTKLV 360

Query: 361 PWRILPYVY 370
           PWRILPYVY
Sbjct: 361 PWRILPYVY 369

[Close]

BLAST of Lsi03G019430 vs. NCBI nr
Match: XP_008442795.1 (PREDICTED: LOW QUALITY PROTEIN: delta(14)-sterol reductase [Cucumis melo])

HSP 1 Score: 740.3 bits (1910), Expect = 7.8e-210
Identity = 363/369 (98.37%), Postives = 366/369 (99.19%), Query Frame = 0


Query: 1   MDLGFLLQASIPSWEAVAILFIFFFYLAIAGSVLPGRIIPGVILSDRTRLQYRCNGLLSL 60
           MDLGFL QASIPSWEAVAILFIFFFYLAIAGSVLPGR+IPGVILSDRTRLQYRCNGLLSL
Sbjct: 1   MDLGFLFQASIPSWEAVAILFIFFFYLAIAGSVLPGRVIPGVILSDRTRLQYRCNGLLSL 60

Query: 61  LVLVALLGVGVKMNYISPTAISDRGLELLSATFWFSVLATFLLCVAGYSSSSQSASLKPH 120
           LVLVALLGVGVKMNYISPTAISDRGLELLSATFWFSVLATFLLCVAGYSSSSQSASLKPH
Sbjct: 61  LVLVALLGVGVKMNYISPTAISDRGLELLSATFWFSVLATFLLCVAGYSSSSQSASLKPH 120

Query: 121 ITGNFIHDWWFGIQLNPHFLGIDLKFFFVRAGMMGWLLINLSVLARSVLNATLSQSMILY 180
           ITGNFIHDWWFGIQLNPHFLGIDLKFFFVRAGMMGWLLINLSVLARSVLNATLSQSMILY
Sbjct: 121 ITGNFIHDWWFGIQLNPHFLGIDLKFFFVRAGMMGWLLINLSVLARSVLNATLSQSMILY 180

Query: 181 QTFCAIYVLDYFVHEEYMTSTWDIIAERLGFMLVFGDLVWIPFTFSIQGWWLLTNKAELT 240
           QTFCAIYVLDYFVHEEYMTSTWDIIAERLGFMLVFGDLVWIPFTFSIQGWWLLTNKAELT
Sbjct: 181 QTFCAIYVLDYFVHEEYMTSTWDIIAERLGFMLVFGDLVWIPFTFSIQGWWLLTNKAELT 240

Query: 241 TAATIANCLVFLIGYLVFRGSNKQKHMFKKNNKVLIWGKPPKVVGGKLLASGYWGIARHC 300
           TAATIANCL+F +GYLVFRGSNKQKHMFKKNNK LIWGKPPKVVGGKLLASGYWGIARHC
Sbjct: 241 TAATIANCLIFXLGYLVFRGSNKQKHMFKKNNKALIWGKPPKVVGGKLLASGYWGIARHC 300

Query: 301 NYLGDLLLALSFSLPCGISSPIPYFYPMYLLILLIWRERRDEARCAEKYKDIWAEYTKLV 360
           NYLGDLLLALSFSLPCGISSPIPYFYPMYLLILLIWRERRDEARCAEKYKDIWAEYTKLV
Sbjct: 301 NYLGDLLLALSFSLPCGISSPIPYFYPMYLLILLIWRERRDEARCAEKYKDIWAEYTKLV 360

Query: 361 PWRILPYVY 370
           PWRILPYVY
Sbjct: 361 PWRILPYVY 369

[Close]

BLAST of Lsi03G019430 vs. NCBI nr
Match: XP_038904165.1 (delta(14)-sterol reductase isoform X1 [Benincasa hispida])

HSP 1 Score: 736.9 bits (1901), Expect = 8.7e-209
Identity = 357/369 (96.75%), Postives = 368/369 (99.73%), Query Frame = 0


Query: 1   MDLGFLLQASIPSWEAVAILFIFFFYLAIAGSVLPGRIIPGVILSDRTRLQYRCNGLLSL 60
           MDLGFLLQASIPSWEAVAILFIFFFYLAIAGSVLPGR+IPGVILSDRTRLQYRCNGLLSL
Sbjct: 1   MDLGFLLQASIPSWEAVAILFIFFFYLAIAGSVLPGRVIPGVILSDRTRLQYRCNGLLSL 60

Query: 61  LVLVALLGVGVKMNYISPTAISDRGLELLSATFWFSVLATFLLCVAGYSSSSQSASLKPH 120
           LVL+ALLGVGVKMNYISPTAISDRGLELLSATFWFSVLATFLLCVAGYSSSSQS+SLKP+
Sbjct: 61  LVLIALLGVGVKMNYISPTAISDRGLELLSATFWFSVLATFLLCVAGYSSSSQSSSLKPY 120

Query: 121 ITGNFIHDWWFGIQLNPHFLGIDLKFFFVRAGMMGWLLINLSVLARSVLNATLSQSMILY 180
           +TGNF+HDWWFGIQLNPHFLGIDLKFFFVRAGMMGWLLINLS+LARS+LNATLSQSMILY
Sbjct: 121 VTGNFVHDWWFGIQLNPHFLGIDLKFFFVRAGMMGWLLINLSILARSILNATLSQSMILY 180

Query: 181 QTFCAIYVLDYFVHEEYMTSTWDIIAERLGFMLVFGDLVWIPFTFSIQGWWLLTNKAELT 240
           QTFCAIYVLDYF+HEEYMTSTWDIIAERLGFMLVFGDLVWIPFTFSIQGWWLLTNKAELT
Sbjct: 181 QTFCAIYVLDYFIHEEYMTSTWDIIAERLGFMLVFGDLVWIPFTFSIQGWWLLTNKAELT 240

Query: 241 TAATIANCLVFLIGYLVFRGSNKQKHMFKKNNKVLIWGKPPKVVGGKLLASGYWGIARHC 300
           TAATIANCLVF+IGYLVFRGSNKQKHMFKKNNK LIWGKPPKVVGGKLLASGYWGIARHC
Sbjct: 241 TAATIANCLVFVIGYLVFRGSNKQKHMFKKNNKALIWGKPPKVVGGKLLASGYWGIARHC 300

Query: 301 NYLGDLLLALSFSLPCGISSPIPYFYPMYLLILLIWRERRDEARCAEKYKDIWAEYTKLV 360
           NYLGDLLLALSFSLPCGISSPIPYFYPMYLLILLIWRERRD+ARCAEKYKDIWAEYTKLV
Sbjct: 301 NYLGDLLLALSFSLPCGISSPIPYFYPMYLLILLIWRERRDDARCAEKYKDIWAEYTKLV 360

Query: 361 PWRILPYVY 370
           PWRILPYVY
Sbjct: 361 PWRILPYVY 369

[Close]

BLAST of Lsi03G019430 vs. NCBI nr
Match: XP_004137867.1 (delta(14)-sterol reductase isoform X1 [Cucumis sativus] >KGN59053.1 hypothetical protein Csa_002529 [Cucumis sativus])

HSP 1 Score: 736.1 bits (1899), Expect = 1.5e-208
Identity = 361/369 (97.83%), Postives = 366/369 (99.19%), Query Frame = 0


Query: 1   MDLGFLLQASIPSWEAVAILFIFFFYLAIAGSVLPGRIIPGVILSDRTRLQYRCNGLLSL 60
           MDLGFLLQASIPSWEAVAILFIFFFYLAIAGSVLPGR+IPGVILSDR+RLQYRCNGLLSL
Sbjct: 1   MDLGFLLQASIPSWEAVAILFIFFFYLAIAGSVLPGRVIPGVILSDRSRLQYRCNGLLSL 60

Query: 61  LVLVALLGVGVKMNYISPTAISDRGLELLSATFWFSVLATFLLCVAGYSSSSQSASLKPH 120
           LVLVALLGVGVKMNYISPTAI+DRGLELLSATFWFSVLATFLLCVAGYSSSSQSASLKPH
Sbjct: 61  LVLVALLGVGVKMNYISPTAIADRGLELLSATFWFSVLATFLLCVAGYSSSSQSASLKPH 120

Query: 121 ITGNFIHDWWFGIQLNPHFLGIDLKFFFVRAGMMGWLLINLSVLARSVLNATLSQSMILY 180
           IT NFIHDWWFG+QLNPHFLGIDLKFFFVRAGMMGWLLINLSVLARSVLNATLSQSMILY
Sbjct: 121 ITRNFIHDWWFGMQLNPHFLGIDLKFFFVRAGMMGWLLINLSVLARSVLNATLSQSMILY 180

Query: 181 QTFCAIYVLDYFVHEEYMTSTWDIIAERLGFMLVFGDLVWIPFTFSIQGWWLLTNKAELT 240
           QTFCAIYVLDYFVHEEYMTSTWDIIAERLGFMLVFGDLVWIPFTFSIQGWWLLTNK ELT
Sbjct: 181 QTFCAIYVLDYFVHEEYMTSTWDIIAERLGFMLVFGDLVWIPFTFSIQGWWLLTNKVELT 240

Query: 241 TAATIANCLVFLIGYLVFRGSNKQKHMFKKNNKVLIWGKPPKVVGGKLLASGYWGIARHC 300
           TAATIANCLVFLIGYLVFRGSNKQKHMFKKNNK LIWGKPPKVVGGKLLASGYWGIARHC
Sbjct: 241 TAATIANCLVFLIGYLVFRGSNKQKHMFKKNNKALIWGKPPKVVGGKLLASGYWGIARHC 300

Query: 301 NYLGDLLLALSFSLPCGISSPIPYFYPMYLLILLIWRERRDEARCAEKYKDIWAEYTKLV 360
           NYLGDLLLALSFSLPCGISSP+PYFYPMYLLILLIWRERRDEARCAEKYKDIWAEYTKLV
Sbjct: 301 NYLGDLLLALSFSLPCGISSPVPYFYPMYLLILLIWRERRDEARCAEKYKDIWAEYTKLV 360

Query: 361 PWRILPYVY 370
           PWRILPYVY
Sbjct: 361 PWRILPYVY 369

[Close]

BLAST of Lsi03G019430 vs. NCBI nr
Match: XP_023526298.1 (delta(14)-sterol reductase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 723.8 bits (1867), Expect = 7.6e-205
Identity = 355/369 (96.21%), Postives = 362/369 (98.10%), Query Frame = 0


Query: 1   MDLGFLLQASIPSWEAVAILFIFFFYLAIAGSVLPGRIIPGVILSDRTRLQYRCNGLLSL 60
           MDL  LLQ  IPSWEAVAILFIFFFYLAIAGSVLPGRIIPGVILSDRTRLQYRCNGLLSL
Sbjct: 1   MDLDSLLQVLIPSWEAVAILFIFFFYLAIAGSVLPGRIIPGVILSDRTRLQYRCNGLLSL 60

Query: 61  LVLVALLGVGVKMNYISPTAISDRGLELLSATFWFSVLATFLLCVAGYSSSSQSASLKPH 120
           L+LVALLG+GVKMNYISPTAISDRGLELLSATFWFSVLATFLLC+AGYSSSSQS+SLKP 
Sbjct: 61  LLLVALLGIGVKMNYISPTAISDRGLELLSATFWFSVLATFLLCIAGYSSSSQSSSLKPR 120

Query: 121 ITGNFIHDWWFGIQLNPHFLGIDLKFFFVRAGMMGWLLINLSVLARSVLNATLSQSMILY 180
            TGNFIHDWWFGIQLNPHFLGIDLKFFFVRAGMMGWLLINLSVLARSVLNATLSQSMILY
Sbjct: 121 ATGNFIHDWWFGIQLNPHFLGIDLKFFFVRAGMMGWLLINLSVLARSVLNATLSQSMILY 180

Query: 181 QTFCAIYVLDYFVHEEYMTSTWDIIAERLGFMLVFGDLVWIPFTFSIQGWWLLTNKAELT 240
           QTFCAIYV+DYFVHEEYMTSTWDIIAERLGFMLVFGDLVWIPFTFSIQGWWLLTN+AELT
Sbjct: 181 QTFCAIYVIDYFVHEEYMTSTWDIIAERLGFMLVFGDLVWIPFTFSIQGWWLLTNRAELT 240

Query: 241 TAATIANCLVFLIGYLVFRGSNKQKHMFKKNNKVLIWGKPPKVVGGKLLASGYWGIARHC 300
           TAATIANCLVFLIGYLVFRGSNKQKHMFKKN+K LIWGKPPKVVGGKLLASGYWGIARHC
Sbjct: 241 TAATIANCLVFLIGYLVFRGSNKQKHMFKKNSKALIWGKPPKVVGGKLLASGYWGIARHC 300

Query: 301 NYLGDLLLALSFSLPCGISSPIPYFYPMYLLILLIWRERRDEARCAEKYKDIWAEYTKLV 360
           NYLGDLLLALSFSLPCGISSPIPYFYPMYLLILLIWRERRDEARCAEKYKDIWAEYTKLV
Sbjct: 301 NYLGDLLLALSFSLPCGISSPIPYFYPMYLLILLIWRERRDEARCAEKYKDIWAEYTKLV 360

Query: 361 PWRILPYVY 370
           PWRILPYVY
Sbjct: 361 PWRILPYVY 369

[Close]

BLAST of Lsi03G019430 vs. TAIR 10
Match: AT3G52940.1 (Ergosterol biosynthesis ERG4/ERG24 family )

HSP 1 Score: 587.8 bits (1514), Expect = 6.1e-168
Identity = 278/369 (75.34%), Postives = 322/369 (87.26%), Query Frame = 0


Query: 1   MDLGFLLQASIPSWEAVAILFIFFFYLAIAGSVLPGRIIPGVILSDRTRLQYRCNGLLSL 60
           MDLG LL    PS ++V +L  +F YLA+AG +LPG++I GV+LSD ++L+YRCNGLL+L
Sbjct: 5   MDLGVLL----PSLQSVYVLVFYFVYLAVAGEILPGKVIRGVLLSDGSQLRYRCNGLLAL 64

Query: 61  LVLVALLGVGVKMNYISPTAISDRGLELLSATFWFSVLATFLLCVAGYSSSSQSASLKPH 120
           ++LVA+LG+  K+  +SP  ++DRGLELLSATF F VL T  L V G SSS++ +SLKPH
Sbjct: 65  ILLVAILGICAKLGIVSPLVVADRGLELLSATFIFCVLVTLALYVTGRSSSNKGSSLKPH 124

Query: 121 ITGNFIHDWWFGIQLNPHFLGIDLKFFFVRAGMMGWLLINLSVLARSVLNATLSQSMILY 180
           ++GN +HDWWFGIQLNP F+ IDLKFFFVRAGMMGWLLINLS+LA+SV + +LSQSMILY
Sbjct: 125 VSGNLVHDWWFGIQLNPQFMSIDLKFFFVRAGMMGWLLINLSILAKSVQDGSLSQSMILY 184

Query: 181 QTFCAIYVLDYFVHEEYMTSTWDIIAERLGFMLVFGDLVWIPFTFSIQGWWLLTNKAELT 240
           Q FCA+Y+LDYFVHEEYMTSTWDIIAERLGFMLVFGDL+WIPFTFSIQGWWLL NK ELT
Sbjct: 185 QIFCALYILDYFVHEEYMTSTWDIIAERLGFMLVFGDLLWIPFTFSIQGWWLLHNKVELT 244

Query: 241 TAATIANCLVFLIGYLVFRGSNKQKHMFKKNNKVLIWGKPPKVVGGKLLASGYWGIARHC 300
             A + NCLVFLIGY+VFRG+NKQKH+FKKN K  IWGKPP VVGGKLL SGYWGIARHC
Sbjct: 245 VPAIVVNCLVFLIGYMVFRGANKQKHIFKKNPKTPIWGKPPVVVGGKLLVSGYWGIARHC 304

Query: 301 NYLGDLLLALSFSLPCGISSPIPYFYPMYLLILLIWRERRDEARCAEKYKDIWAEYTKLV 360
           NYLGDL+LALSFSLPCGISSP+PYFYP+YLLILLIWRERRDE RCAEKYK+IWAEY +LV
Sbjct: 305 NYLGDLMLALSFSLPCGISSPVPYFYPIYLLILLIWRERRDEVRCAEKYKEIWAEYLRLV 364

Query: 361 PWRILPYVY 370
           PWRILPYVY
Sbjct: 365 PWRILPYVY 369

[Close]

BLAST of Lsi03G019430 vs. TAIR 10
Match: AT3G52940.2 (Ergosterol biosynthesis ERG4/ERG24 family )

HSP 1 Score: 330.1 bits (845), Expect = 2.3e-90
Identity = 160/230 (69.57%), Postives = 195/230 (84.78%), Query Frame = 0


Query: 1   MDLGFLLQASIPSWEAVAILFIFFFYLAIAGSVLPGRIIPGVILSDRTRLQYRCNGLLSL 60
           MDLG LL    PS ++V +L  +F YLA+AG +LPG++I GV+LSD ++L+YRCNGLL+L
Sbjct: 5   MDLGVLL----PSLQSVYVLVFYFVYLAVAGEILPGKVIRGVLLSDGSQLRYRCNGLLAL 64

Query: 61  LVLVALLGVGVKMNYISPTAISDRGLELLSATFWFSVLATFLLCVAGYSSSSQSASLKPH 120
           ++LVA+LG+  K+  +SP  ++DRGLELLSATF F VL T  L V G SSS++ +SLKPH
Sbjct: 65  ILLVAILGICAKLGIVSPLVVADRGLELLSATFIFCVLVTLALYVTGRSSSNKGSSLKPH 124

Query: 121 ITGNFIHDWWFGIQLNPHFLGIDLKFFFVRAGMMGWLLINLSVLARSVLNATLSQSMILY 180
           ++GN +HDWWFGIQLNP F+ IDLKFFFVRAGMMGWLLINLS+LA+SV + +LSQSMILY
Sbjct: 125 VSGNLVHDWWFGIQLNPQFMSIDLKFFFVRAGMMGWLLINLSILAKSVQDGSLSQSMILY 184

Query: 181 QTFCAIYVLDYFVHEEYMTSTWDIIAERLGFMLVFGDLVWIPFTFSIQGW 231
           Q FCA+Y+LDYFVHEEYMTSTWDIIAERLGFMLVFGDL+WIPFTFSIQ W
Sbjct: 185 QIFCALYILDYFVHEEYMTSTWDIIAERLGFMLVFGDLLWIPFTFSIQAW 230

[Close]

BLAST of Lsi03G019430 vs. TAIR 10
Match: AT1G50430.2 (Ergosterol biosynthesis ERG4/ERG24 family )

HSP 1 Score: 166.0 bits (419), Expect = 5.7e-41
Identity = 114/374 (30.48%), Postives = 193/374 (51.60%), Query Frame = 0


Query: 12  PSWEAVAILFIFFFYLAIAGSVLPGRIIPGVILSDRTRLQYRCNGLLSLLVLVALLGVGV 71
           P+  A  I+F +  + AI   +LPG+ + G I     R  Y+ NGL +  V +A   +G+
Sbjct: 62  PTLIAWKIIFCYGAFEAILQLLLPGKRVEGPISPAGNRPVYKANGLAAYFVTLATY-LGL 121

Query: 72  KMNYISPTAISDRGLELLSATFWFSVLATFLLCVAGYSSSSQSASLKPHITGNFIHDWWF 131
                +P  + D   E+ SA  + S +   LL + G+ + S S S      GN I D+++
Sbjct: 122 WFGIFNPAIVYDHLGEIFSALIFGSFIFCVLLYIKGHVAPSSSDS---GSCGNLIIDFYW 181

Query: 132 GIQLNPHF-LGIDLKFFF-VRAGMMGWLLINLSVLARSV-LNATLSQSMILYQTFCAIYV 191
           G++L P      D+K F   R GMM W ++ ++   +   +N  +S SM++      +YV
Sbjct: 182 GMELYPRIGKSFDIKVFTNCRFGMMSWAVLAVTYCIKQYEINGKVSDSMLVNTILMLVYV 241

Query: 192 LDYFVHEEYMTSTWDIIAERLGFMLVFGDLVWIPFTFSIQGWWLLTNKAELTTAATIANC 251
             +F  E    +T DI  +R GF + +G LVW+P  ++  G +L+ +  EL T   I   
Sbjct: 242 TKFFWWEAGYWNTMDIAHDRAGFYICWGCLVWVPSVYTSPGMYLVNHPVELGTQLAIYIL 301

Query: 252 LVFLIGYLVFRGSNKQKHMFKK-NNKVLIWGK-PPKVVG-----------GKLLASGYWG 311
           +  ++   +    ++Q+  F++ N K L+WG+ P K+V              LL SG+WG
Sbjct: 302 VAGILCIYINYDCDRQRQEFRRTNGKCLVWGRAPSKIVASYTTTSGETKTSLLLTSGWWG 361

Query: 312 IARHCNYLGDLLLALSFSLPCGISSPIPYFYPMYLLILLIWRERRDEARCAEKYKDIWAE 370
           +ARH +Y+ ++L A  +++P    + + YFY ++L +LL  R +RD+ RC  KY   W  
Sbjct: 362 LARHFHYVPEILSAFFWTVPALFDNFLAYFYVIFLTLLLFDRAKRDDDRCRSKYGKYWKL 421

[Close]

BLAST of Lsi03G019430 vs. TAIR 10
Match: AT1G50430.1 (Ergosterol biosynthesis ERG4/ERG24 family )

HSP 1 Score: 162.2 bits (409), Expect = 8.2e-40
Identity = 113/374 (30.21%), Postives = 190/374 (50.80%), Query Frame = 0


Query: 12  PSWEAVAILFIFFFYLAIAGSVLPGRIIPGVILSDRTRLQYRCNGLLSLLVLVALLGVGV 71
           P+  A  I+F +  + AI   +LPG+ + G I     R  Y+ NGL +  V +A      
Sbjct: 62  PTLIAWKIIFCYGAFEAILQLLLPGKRVEGPISPAGNRPVYKANGLAAYFVTLATYLGLW 121

Query: 72  KMNYISPTAISDRGLELLSATFWFSVLATFLLCVAGYSSSSQSASLKPHITGNFIHDWWF 131
                +P  + D   E+ SA  + S +   LL + G+ + S S S      GN I D+++
Sbjct: 122 WFGIFNPAIVYDHLGEIFSALIFGSFIFCVLLYIKGHVAPSSSDS---GSCGNLIIDFYW 181

Query: 132 GIQLNPHF-LGIDLKFFF-VRAGMMGWLLINLSVLARSV-LNATLSQSMILYQTFCAIYV 191
           G++L P      D+K F   R GMM W ++ ++   +   +N  +S SM++      +YV
Sbjct: 182 GMELYPRIGKSFDIKVFTNCRFGMMSWAVLAVTYCIKQYEINGKVSDSMLVNTILMLVYV 241

Query: 192 LDYFVHEEYMTSTWDIIAERLGFMLVFGDLVWIPFTFSIQGWWLLTNKAELTTAATIANC 251
             +F  E    +T DI  +R GF + +G LVW+P  ++  G +L+ +  EL T   I   
Sbjct: 242 TKFFWWEAGYWNTMDIAHDRAGFYICWGCLVWVPSVYTSPGMYLVNHPVELGTQLAIYIL 301

Query: 252 LVFLIGYLVFRGSNKQKHMFKK-NNKVLIWGK-PPKVVG-----------GKLLASGYWG 311
           +  ++   +    ++Q+  F++ N K L+WG+ P K+V              LL SG+WG
Sbjct: 302 VAGILCIYINYDCDRQRQEFRRTNGKCLVWGRAPSKIVASYTTTSGETKTSLLLTSGWWG 361

Query: 312 IARHCNYLGDLLLALSFSLPCGISSPIPYFYPMYLLILLIWRERRDEARCAEKYKDIWAE 370
           +ARH +Y+ ++L A  +++P    + + YFY ++L +LL  R +RD+ RC  KY   W  
Sbjct: 362 LARHFHYVPEILSAFFWTVPALFDNFLAYFYVIFLTLLLFDRAKRDDDRCRSKYGKYWKL 421

The following BLAST results are available for this feature:

BLAST of Lsi03G019430 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LDR4	8.5e-167	75.34	Delta(14)-sterol reductase OS=Arabidopsis thaliana OX=3702 GN=FK PE=1 SV=2	[more]
G4SW86	6.7e-71	40.66	Delta(14)-sterol reductase OS=Methylomicrobium alcaliphilum (strain DSM 19304 / ...	[more]
Q71KT5	2.7e-64	40.77	Delta(14)-sterol reductase TM7SF2 OS=Mus musculus OX=10090 GN=Tm7sf2 PE=1 SV=2	[more]
O08984	4.8e-61	39.18	Delta(14)-sterol reductase LBR OS=Rattus norvegicus OX=10116 GN=Lbr PE=1 SV=1	[more]
Q3U9G9	8.2e-61	39.30	Delta(14)-sterol reductase LBR OS=Mus musculus OX=10090 GN=Lbr PE=1 SV=2	[more]

BLAST of Lsi03G019430 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7TKR7	5.9e-211	98.92	Delta(14)-sterol reductase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaff...	[more]
A0A1S3B6J9	3.8e-210	98.37	LOW QUALITY PROTEIN: delta(14)-sterol reductase OS=Cucumis melo OX=3656 GN=LOC10...	[more]
A0A0A0LB94	7.2e-209	97.83	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G748820 PE=3 SV=1	[more]
A0A6J1F6L0	1.4e-204	95.93	delta(14)-sterol reductase-like OS=Cucurbita moschata OX=3662 GN=LOC111441342 PE...	[more]
A0A6J1J0W9	9.1e-204	95.12	delta(14)-sterol reductase-like OS=Cucurbita maxima OX=3661 GN=LOC111481674 PE=3...	[more]

BLAST of Lsi03G019430 vs. NCBI nr


Match Name	E-value	Identity	Description
KAA0043924.1	1.2e-210	98.92	delta(14)-sterol reductase [Cucumis melo var. makuwa] >TYK25215.1 delta(14)-ster...	[more]
XP_008442795.1	7.8e-210	98.37	PREDICTED: LOW QUALITY PROTEIN: delta(14)-sterol reductase [Cucumis melo]	[more]
XP_038904165.1	8.7e-209	96.75	delta(14)-sterol reductase isoform X1 [Benincasa hispida]	[more]
XP_004137867.1	1.5e-208	97.83	delta(14)-sterol reductase isoform X1 [Cucumis sativus] >KGN59053.1 hypothetical...	[more]
XP_023526298.1	7.6e-205	96.21	delta(14)-sterol reductase-like [Cucurbita pepo subsp. pepo]	[more]

BLAST of Lsi03G019430 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G52940.1	6.1e-168	75.34	Ergosterol biosynthesis ERG4/ERG24 family	[more]
AT3G52940.2	2.3e-90	69.57	Ergosterol biosynthesis ERG4/ERG24 family	[more]
AT1G50430.2	5.7e-41	30.48	Ergosterol biosynthesis ERG4/ERG24 family	[more]
AT1G50430.1	8.2e-40	30.21	Ergosterol biosynthesis ERG4/ERG24 family	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001171	Ergosterol biosynthesis ERG4/ERG24	PFAM	PF01222	ERG4_ERG24	coord: 10..369 e-value: 1.6E-89 score: 300.7
None	No IPR available	GENE3D	1.20.120.1630		coord: 170..368 e-value: 9.4E-22 score: 79.7
None	No IPR available	PANTHER	PTHR21257	DELTA(14)-STEROL REDUCTASE	coord: 1..369
None	No IPR available	PANTHER	PTHR21257:SF51	BNACNNG50210D PROTEIN	coord: 1..369
IPR018083	Sterol reductase, conserved site	PROSITE	PS01018	STEROL_REDUCT_2	coord: 333..356

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi03G019430.1	Lsi03G019430.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016126	sterol biosynthetic process
cellular_component	GO:0030176	integral component of endoplasmic reticulum membrane
cellular_component	GO:0016020	membrane
molecular_function	GO:0050613	delta14-sterol reductase activity
molecular_function	GO:0016628	oxidoreductase activity, acting on the CH-CH group of donors, NAD or NADP as acceptor