Lsi03G017440 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi03G017440
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	Growth-regulating factor
Location	chr03: 28812520 .. 28814435 (-)
RNA-Seq Expression	Lsi03G017440
Synteny	Lsi03G017440

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

CCACCTCCTCTCCGGCGGCCGCGATATGCGAATCAGAAAACGCCAGGTCCCATTACCCTTCTCTTCTCTCTCTCCTCTCCCTCTTTCAGATCCCCTCCTCCACCAATCTCCGCCGGTGCAACTCCCTCCCTCCTCCGATCCTCCAAATCTACACCCCTTCCCCTCCGATAATGCCTTGGTTAGTAATCCCAACTCCAACTCTCGACCTTTTCTTTCTTTTGATTTCGATTTCGGATTTTATCTTTCTTCGTTTCCTCTTCAAAATTGCACCGAGGATATACTCGTTCGTTAAATTAATGTATGTTTATCACTGATCAACTCATGGGGTTGTGGGATTTTTGTGTGTAGGAGAAGAATCAGAAGCTACAACAACATGATTCCAGGTAACAAACTAAAATCCCCCATCTCTCTGTTCGTTCTTCCTTCTTTCTCCAACAACTAGTAAAAAAGTTAAAAAATAGAAATAAAAATAAAAATAAAAATTATTTTTTTTTTTTAATAATGAGTATCCAATAAGAATAGAGGAAAAAAGGGGGGGCAAGTGGAAATACTGTTGGGATATTTTTATATTATTTTTCATGACTCTATTGTGCGCCCATGGGCTCTGCATGTTCTTCACTGGTCCACAGCTCTCTCCTTCCTCTTCTTCTGAACTTCTGATCATCACTTACAGTAATTACACTTATTCTTTTGGTTTTCCTTTCCATTTATTTATTTATTTATTTTGCAATAATACAGTAGTAGACTAGCTAGCTATAACTATTACTAATTAATGTTGAGTTATGCGTAATTGTTTTTGGGTTTGGTTTTAGGGAAACAATTGTGATTGGTGCAGAAACAACCAATCATGGCGTTCTTCCACAATCCCCCTCTTCTTCTCAAGGTCATTCCATTTTTAATTTCTCCCCTTTATCATTTCTGGGCTGTTTGCCTTCTGCACTCAAATTTATTTGTTCATTCTCTTGCTGATTTCTGACCTTCAAATCCTAATATTATAATTCCATCTTTTCTTAATTCACAGTATTGATTGTGGCCTTATCAATTACTTATATTGTTGGGTTTATAAACTTAAGTGTTATTGGATCGAGTAGTACTTTTCTATGCTATAATTTTTGTGTTTAAATCTACCTATACATCACTTCTTCGCTTTATAATCGATATGAATTAGGGGTATACAGATAGTTTTCTGGAATTTGTGATCAATTCATGGGTGGATTGGATTTGTTTATTTGATGTTATTGTTTGGGTTTATGTTTGATGTAGTAGATGGTGGGCGATGGTGCGAGAGGGAGAAGACATTTCCATTAAAGAAGAGAAGAGGAAGTTTTGAGAGGTCGAGTGACGATCAGGACAACGATGACGAACAAGATGACAACAAGGACGATCAAGTTGTCACCACCATTGTGAATTCACTGTCGGATGAGAAGAAGCCAATGAAAACAAAGGTGAACAGAAAATACACATTGTCACAAAAGCAATCTAAACTGGGTCTTGACCTCGCCGGAAAAAAGAGGTCGAGAGGCGGTGCGTTAATGGAGGGATCTCGGTGTAGTCGTGTCAATGGAAGAGGATGGCGATGCTGCCAACAAACTCTCGTTGGTTATTCTCTCTGTGAACATCACTTGGGAAAAGGTCGTCTCCGTAGCATGAATAATGTCCGCAACCGCTCTTTGGCTGCCGCCATCGCGACAGTACCGAAGAATATCGCCATTGTTGATAAAGATGAGAAGAAGCCATCTCCATTCCCACCACCTCCACCGACATCATTCATGCTGACCAGTAAGAAGAGAATGAAACTGGGTATGGTGAAAGCGAGGTCGATAAGCAGCTTGCTCGGCCAACCCGACGGCATGGTGGTGACACCATCGCCTCCTCCTCCTCCGCCACGGATGTTGTATGATGACAACATTGACGAC

mRNA sequence

CCACCTCCTCTCCGGCGGCCGCGATATGCGAATCAGAAAACGCCAGGTCCCATTACCCTTCTCTTCTCTCTCTCCTCTCCCTCTTTCAGATCCCCTCCTCCACCAATCTCCGCCGGTGCAACTCCCTCCCTCCTCCGATCCTCCAAATCTACACCCCTTCCCCTCCGATAATGCCTTGGTTAGTAATCCCAACTCCAACTCTCGACCTTTTCTTTCTTTTGATTTCGATTTCGGATTTTATCTTTCTTCGTTTCCTCTTCAAAATTGCACCGAGGATATACTCGAGAAGAATCAGAAGCTACAACAACATGATTCCAGGGAAACAATTGTGATTGGTGCAGAAACAACCAATCATGGCGTTCTTCCACAATCCCCCTCTTCTTCTCAAGTATTGATTGTGGCCTTATCAATTACTTATATTGTTGGGTTTATAAACTTAAGTGTTATTGGATCGAGTATAGATGGTGGGCGATGGTGCGAGAGGGAGAAGACATTTCCATTAAAGAAGAGAAGAGGAAGTTTTGAGAGGTCGAGTGACGATCAGGACAACGATGACGAACAAGATGACAACAAGGACGATCAAGTTGTCACCACCATTGTGAATTCACTGTCGGATGAGAAGAAGCCAATGAAAACAAAGGTGAACAGAAAATACACATTGTCACAAAAGCAATCTAAACTGGGTCTTGACCTCGCCGGAAAAAAGAGGTCGAGAGGCGGTGCGTTAATGGAGGGATCTCGGTGTAGTCGTGTCAATGGAAGAGGATGGCGATGCTGCCAACAAACTCTCGTTGGTTATTCTCTCTGTGAACATCACTTGGGAAAAGGTCGTCTCCGTAGCATGAATAATGTCCGCAACCGCTCTTTGGCTGCCGCCATCGCGACAGTACCGAAGAATATCGCCATTGTTGATAAAGATGAGAAGAAGCCATCTCCATTCCCACCACCTCCACCGACATCATTCATGCTGACCAGTAAGAAGAGAATGAAACTGGGTATGGTGAAAGCGAGGTCGATAAGCAGCTTGCTCGGCCAACCCGACGGCATGGTGGTGACACCATCGCCTCCTCCTCCTCCGCCACGGATGTTGTATGATGACAACATTGACGAC

Coding sequence (CDS)

ATGCGAATCAGAAAACGCCAGGTCCCATTACCCTTCTCTTCTCTCTCTCCTCTCCCTCTTTCAGATCCCCTCCTCCACCAATCTCCGCCGGTGCAACTCCCTCCCTCCTCCGATCCTCCAAATCTACACCCCTTCCCCTCCGATAATGCCTTGGTTAGTAATCCCAACTCCAACTCTCGACCTTTTCTTTCTTTTGATTTCGATTTCGGATTTTATCTTTCTTCGTTTCCTCTTCAAAATTGCACCGAGGATATACTCGAGAAGAATCAGAAGCTACAACAACATGATTCCAGGGAAACAATTGTGATTGGTGCAGAAACAACCAATCATGGCGTTCTTCCACAATCCCCCTCTTCTTCTCAAGTATTGATTGTGGCCTTATCAATTACTTATATTGTTGGGTTTATAAACTTAAGTGTTATTGGATCGAGTATAGATGGTGGGCGATGGTGCGAGAGGGAGAAGACATTTCCATTAAAGAAGAGAAGAGGAAGTTTTGAGAGGTCGAGTGACGATCAGGACAACGATGACGAACAAGATGACAACAAGGACGATCAAGTTGTCACCACCATTGTGAATTCACTGTCGGATGAGAAGAAGCCAATGAAAACAAAGGTGAACAGAAAATACACATTGTCACAAAAGCAATCTAAACTGGGTCTTGACCTCGCCGGAAAAAAGAGGTCGAGAGGCGGTGCGTTAATGGAGGGATCTCGGTGTAGTCGTGTCAATGGAAGAGGATGGCGATGCTGCCAACAAACTCTCGTTGGTTATTCTCTCTGTGAACATCACTTGGGAAAAGGTCGTCTCCGTAGCATGAATAATGTCCGCAACCGCTCTTTGGCTGCCGCCATCGCGACAGTACCGAAGAATATCGCCATTGTTGATAAAGATGAGAAGAAGCCATCTCCATTCCCACCACCTCCACCGACATCATTCATGCTGACCAGTAAGAAGAGAATGAAACTGGGTATGGTGAAAGCGAGGTCGATAAGCAGCTTGCTCGGCCAACCCGACGGCATGGTGGTGACACCATCGCCTCCTCCTCCTCCGCCACGGATGTTGTATGATGACAACATTGACGAC

Protein sequence

MRIRKRQVPLPFSSLSPLPLSDPLLHQSPPVQLPPSSDPPNLHPFPSDNALVSNPNSNSRPFLSFDFDFGFYLSSFPLQNCTEDILEKNQKLQQHDSRETIVIGAETTNHGVLPQSPSSSQVLIVALSITYIVGFINLSVIGSSIDGGRWCEREKTFPLKKRRGSFERSSDDQDNDDEQDDNKDDQVVTTIVNSLSDEKKPMKTKVNRKYTLSQKQSKLGLDLAGKKRSRGGALMEGSRCSRVNGRGWRCCQQTLVGYSLCEHHLGKGRLRSMNNVRNRSLAAAIATVPKNIAIVDKDEKKPSPFPPPPPTSFMLTSKKRMKLGMVKARSISSLLGQPDGMVVTPSPPPPPPRMLYDDNIDD

Homology

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BLAST of Lsi03G017440 vs. ExPASy Swiss-Prot
Match: Q6AWY1 (Growth-regulating factor 8 OS=Oryza sativa subsp. japonica OX=39947 GN=GRF8 PE=2 SV=1)

HSP 1 Score: 46.2 bits (108), Expect = 9.1e-04
Identity = 21/56 (37.50%), Postives = 29/56 (51.79%), Query Frame = 0


Query: 231 GGALMEGSRCSRVNGRGWRCCQQTLVGYSLCEHHLGKGRLRSMNNVRNRSLAAAIA 287
           G A +E  RC R +G+ WRC +  +     CE H+ +GR RS  +V  R     IA
Sbjct: 155 GCADVEPRRCRRTDGKKWRCSRDAVGDQKYCERHINRGRHRSRKHVEGRKATLTIA 210

[Close]

BLAST of Lsi03G017440 vs. ExPASy TrEMBL
Match: A0A6J1F795 (Growth-regulating factor OS=Cucurbita moschata OX=3662 GN=LOC111441497 PE=3 SV=1)

HSP 1 Score: 357.8 bits (917), Expect = 5.2e-95
Identity = 217/364 (59.62%), Postives = 235/364 (64.56%), Query Frame = 0


Query: 1   MRIRKRQVPLPFSSLSPLPLSDPLLHQSPPVQLP---PSSDPPNLHPFPSDNALVSNPNS 60
           MRIRKRQVPLPFSSLSPLPLSDPLL ++PPVQL    PSSDPPNLHP+  D+A       
Sbjct: 1   MRIRKRQVPLPFSSLSPLPLSDPLLRRTPPVQLHLQLPSSDPPNLHPYACDSAAYH---- 60

Query: 61  NSRPFLSFDFDFGFYLSSFPLQNCTEDILEKNQKLQQHDSRETIVIGAETTNHGVLPQSP 120
                                   T + L K  +L+QHDSRET V GAE +N GVLP S 
Sbjct: 61  ------------------------TNNALGKKDQLEQHDSRETTVFGAEASN-GVLPLSS 120

Query: 121 SSSQVLIVALSITYIVGFINLSVIGSSIDGGRWCEREKTFPLKKRRGSFERSSDDQDNDD 180
           SS QV                         G+WCER+K FPLKKRRGSFER SDD  N+ 
Sbjct: 121 SSPQV-----------------------GDGQWCERDKDFPLKKRRGSFERQSDDDQNN- 180

Query: 181 EQDDNKDDQVVTTIVNSLSDEKKPMKTKVNRKYTLSQKQSKLGLDLAGKKRSRGGALMEG 240
             DD          + SL D KK  K K         KQ KLGL+LAGKKRSRGGALMEG
Sbjct: 181 -ADDQTTTAAAAAAMESLPDHKKTTKNK---------KQPKLGLNLAGKKRSRGGALMEG 240

Query: 241 SRCSRVNGRGWRCCQQTLVGYSLCEHHLGKGRLRSMNNVRNRSLAAAIATVPKNIAIVDK 300
           SRCSRVNGRGWRCCQQTLVGYSLCEHHLGKGRLRSMNNVR+RSLAAA AT+ KNI  VDK
Sbjct: 241 SRCSRVNGRGWRCCQQTLVGYSLCEHHLGKGRLRSMNNVRSRSLAAAAATL-KNIVTVDK 300

Query: 301 DEKKPSPFPPPPPTSFMLTSKKRMKLGMVKARSISSLLGQPDGMVVTPSPPPPPPRMLYD 360
           DEKKP P PPPP  S  L SKKR KLG+VKARSISSLLGQPDGM   P PPPP    L +
Sbjct: 301 DEKKPPPCPPPPSPSLTLASKKRTKLGLVKARSISSLLGQPDGMAAIPPPPPPSQSTLPN 300

Query: 361 DNID 362
           DNI+
Sbjct: 361 DNIN 300

[Close]

BLAST of Lsi03G017440 vs. ExPASy TrEMBL
Match: A0A6J1J4K8 (Growth-regulating factor OS=Cucurbita maxima OX=3661 GN=LOC111481709 PE=3 SV=1)

HSP 1 Score: 355.9 bits (912), Expect = 2.0e-94
Identity = 219/364 (60.16%), Postives = 237/364 (65.11%), Query Frame = 0


Query: 1   MRIRKRQVPLPFSSLSPLPLSDPLLHQSPPVQLP---PSSDPPNLHPFPSDNALVSNPNS 60
           MRIRKRQVPL FSSLSPLPLSDPLL ++PPVQL    PSSDPPNLHP+  D+A       
Sbjct: 1   MRIRKRQVPLSFSSLSPLPLSDPLLRRTPPVQLHLQLPSSDPPNLHPYACDSA------- 60

Query: 61  NSRPFLSFDFDFGFYLSSFPLQNCTEDILEKNQKLQQHDSRETIVIGAETTNHGVLPQSP 120
                                 + T + L K  +L+QHDSRET V GAET+N GVLP S 
Sbjct: 61  ---------------------ADHTNNALGKKDQLEQHDSRETTVFGAETSN-GVLPLSS 120

Query: 121 SSSQVLIVALSITYIVGFINLSVIGSSIDGGRWCEREKTFPLKKRRGSFERSSDDQDNDD 180
           SS QV                         G+WCER+K FPLKKRRGSFER SDD  N  
Sbjct: 121 SSPQV-----------------------GDGQWCERDKEFPLKKRRGSFERQSDDDQN-- 180

Query: 181 EQDDNKDDQVVTTIVNSLSDEKKPMKTKVNRKYTLSQKQSKLGLDLAGKKRSRGGALMEG 240
               N DDQ  TT + SL D KK  K K         KQ KLGL+LAGKKRSRGGALMEG
Sbjct: 181 ----NADDQ-TTTAMESLQDHKKTTKNK---------KQPKLGLNLAGKKRSRGGALMEG 240

Query: 241 SRCSRVNGRGWRCCQQTLVGYSLCEHHLGKGRLRSMNNVRNRSLAAAIATVPKNIAIVDK 300
           S CSRVNGRGWRCCQQTLVGYSLCEHHLGKGRLRSMNNVR+RSLAAA AT+ KNI  VDK
Sbjct: 241 SLCSRVNGRGWRCCQQTLVGYSLCEHHLGKGRLRSMNNVRSRSLAAAAATL-KNIVTVDK 295

Query: 301 DEKKPSPFPPPPPTSFMLTSKKRMKLGMVKARSISSLLGQPDGMVVTPSPPPPPPRMLYD 360
           DEKKP P PPPP  S  L SKKR KLG+VKARSISSLLGQPDGM   P  PPP    L +
Sbjct: 301 DEKKPPPCPPPPSPSLTLASKKRTKLGLVKARSISSLLGQPDGMAAIPPSPPPSQSTLPN 295

Query: 361 DNID 362
           DNI+
Sbjct: 361 DNIN 295

[Close]

BLAST of Lsi03G017440 vs. ExPASy TrEMBL
Match: A0A5A7TKD1 (Growth-regulating factor OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold236G00710 PE=3 SV=1)

HSP 1 Score: 342.8 bits (878), Expect = 1.7e-90
Identity = 222/372 (59.68%), Postives = 251/372 (67.47%), Query Frame = 0


Query: 1   MRIRKRQVPLP-FSSLSPLPLSDPLLHQSPPVQL-----PPSSDPPNLHPFPSDNALVSN 60
           MRIRKRQ+PLP FSSLS            PPVQL       SSDPP ++P  ++N     
Sbjct: 1   MRIRKRQLPLPAFSSLS-----------DPPVQLHLPSSSSSSDPP-INPLDNNN----- 60

Query: 61  PNSNSRPFLSFDFDFGFYLSSFPLQNCTEDILEKNQKLQQHDSRETIVIGAETTNHGVLP 120
                                           +K + LQQ+DSRETI+IGAET N+ +LP
Sbjct: 61  --------------------------------QKQKLLQQYDSRETILIGAETINNALLP 120

Query: 121 QSPSSSQVLIVALSITYIVGFINLSVIGSSIDGGRWCEREKTFPLKKRRGSFERSSDDQD 180
            S SSSQV++V              V+G    GGRWCE+EK FPLKKRRGSFERSS D+D
Sbjct: 121 HSSSSSQVVVV------------FDVVG----GGRWCEKEKAFPLKKRRGSFERSS-DED 180

Query: 181 NDDEQDDNKDDQVVTTIVNSLSDEKKPMKTKVNRKYTLSQKQSKLGLDL-AGKKRSRGGA 240
           NDD    NKD QV T     L DEKK +KTK+N+K TLSQKQSK+GLD+  GKKRSRGGA
Sbjct: 181 NDDH---NKDIQVAT----KLQDEKKMIKTKMNKKCTLSQKQSKVGLDIVVGKKRSRGGA 240

Query: 241 LMEGSRCSRVNGRGWRCCQQTLVGYSLCEHHLGKGRLRSMNNVRNRSLAAAIATVPKNIA 300
           LMEGSRCSRVNGRGWRCCQQTLVGYSLCEHHLGKGRLRSMNNVRNRSLAAA    PKNI 
Sbjct: 241 LMEGSRCSRVNGRGWRCCQQTLVGYSLCEHHLGKGRLRSMNNVRNRSLAAA---TPKNIV 293

Query: 301 IVDKDEKKPSP--FP--PPPPTSFMLTSKKRMKLGMVKARSISSLLGQPDGMVVTPSPPP 360
           +++KDEKKPSP  FP  PPPP+SF LT+KKRMKLG+VKARSISSLLGQPDG+V TP    
Sbjct: 301 VIEKDEKKPSPSQFPSSPPPPSSFSLTNKKRMKLGVVKARSISSLLGQPDGIVATPL--- 293

Query: 361 PPPRMLYDDNID 362
             P  L+DDNID
Sbjct: 361 SLPSTLHDDNID 293

[Close]

BLAST of Lsi03G017440 vs. ExPASy TrEMBL
Match: A0A1S3B737 (Growth-regulating factor OS=Cucumis melo OX=3656 GN=LOC103486754 PE=3 SV=1)

HSP 1 Score: 342.8 bits (878), Expect = 1.7e-90
Identity = 222/372 (59.68%), Postives = 251/372 (67.47%), Query Frame = 0


Query: 1   MRIRKRQVPLP-FSSLSPLPLSDPLLHQSPPVQL-----PPSSDPPNLHPFPSDNALVSN 60
           MRIRKRQ+PLP FSSLS            PPVQL       SSDPP ++P  ++N     
Sbjct: 1   MRIRKRQLPLPAFSSLS-----------DPPVQLHLPSSSSSSDPP-INPLDNNN----- 60

Query: 61  PNSNSRPFLSFDFDFGFYLSSFPLQNCTEDILEKNQKLQQHDSRETIVIGAETTNHGVLP 120
                                           +K + LQQ+DSRETI+IGAET N+ +LP
Sbjct: 61  --------------------------------QKQKLLQQYDSRETILIGAETINNALLP 120

Query: 121 QSPSSSQVLIVALSITYIVGFINLSVIGSSIDGGRWCEREKTFPLKKRRGSFERSSDDQD 180
            S SSSQV++V              V+G    GGRWCE+EK FPLKKRRGSFERSS D+D
Sbjct: 121 HSSSSSQVVVV------------FDVVG----GGRWCEKEKAFPLKKRRGSFERSS-DED 180

Query: 181 NDDEQDDNKDDQVVTTIVNSLSDEKKPMKTKVNRKYTLSQKQSKLGLDL-AGKKRSRGGA 240
           NDD    NKD QV T     L DEKK +KTK+N+K TLSQKQSK+GLD+  GKKRSRGGA
Sbjct: 181 NDDH---NKDIQVAT----KLQDEKKMIKTKMNKKCTLSQKQSKVGLDIVVGKKRSRGGA 240

Query: 241 LMEGSRCSRVNGRGWRCCQQTLVGYSLCEHHLGKGRLRSMNNVRNRSLAAAIATVPKNIA 300
           LMEGSRCSRVNGRGWRCCQQTLVGYSLCEHHLGKGRLRSMNNVRNRSLAAA    PKNI 
Sbjct: 241 LMEGSRCSRVNGRGWRCCQQTLVGYSLCEHHLGKGRLRSMNNVRNRSLAAA---TPKNIV 293

Query: 301 IVDKDEKKPSP--FP--PPPPTSFMLTSKKRMKLGMVKARSISSLLGQPDGMVVTPSPPP 360
           +++KDEKKPSP  FP  PPPP+SF LT+KKRMKLG+VKARSISSLLGQPDG+V TP    
Sbjct: 301 VIEKDEKKPSPSQFPSSPPPPSSFSLTNKKRMKLGVVKARSISSLLGQPDGIVATPL--- 293

Query: 361 PPPRMLYDDNID 362
             P  L+DDNID
Sbjct: 361 SLPSTLHDDNID 293

[Close]

BLAST of Lsi03G017440 vs. ExPASy TrEMBL
Match: A0A0A0LGX5 (Growth-regulating factor OS=Cucumis sativus OX=3659 GN=Csa_3G806230 PE=3 SV=1)

HSP 1 Score: 337.0 bits (863), Expect = 9.5e-89
Identity = 219/372 (58.87%), Postives = 242/372 (65.05%), Query Frame = 0


Query: 1   MRIRKRQVPLP-FSSLSPLPLSDPLLHQSPPVQLP-PSSDPPNLHPFPSDNALVSNPNSN 60
           MRIRKRQ+PLP FSSLS            PPVQLP PSSDPP             NP  N
Sbjct: 1   MRIRKRQLPLPTFSSLS-----------DPPVQLPLPSSDPP------------INPLDN 60

Query: 61  SRPFLSFDFDFGFYLSSFPLQNCTEDILEKNQKLQQHDSRETIVIGAETTNHGVLPQSPS 120
           +                           +K + LQQ+DSRETI+IGAET N+ +LP S S
Sbjct: 61  N---------------------------QKQKLLQQYDSRETILIGAETINNALLPHSSS 120

Query: 121 SSQVLIVALSITYIVGFINLSVIGSSIDGGRWCEREKTFPLKKRRGSFERSSDDQDNDDE 180
           S                       SS  GGRWCE+EK FPLKKRRGSFERSS D+DNDD 
Sbjct: 121 S-----------------------SSQGGGRWCEKEKAFPLKKRRGSFERSS-DEDNDDH 180

Query: 181 QDDNKDDQVVTTIVNSLSDEKKPMKTKVNRKYTLSQKQSKLGLD-LAGKKRSRGGALMEG 240
             D+ D QV T     L DEKK MKTK+N+K TLSQKQSK+GLD + GKKRSRGGALMEG
Sbjct: 181 SKDS-DHQVAT----KLQDEKKMMKTKMNKKCTLSQKQSKVGLDAVVGKKRSRGGALMEG 240

Query: 241 SRCSRVNGRGWRCCQQTLVGYSLCEHHLGKGRLRSMNNVRNRSLAAAIATVPKNIAIVDK 300
           SRCSRVNGRGWRCCQQTLVGYSLCEHHLGKGRLRSMNNVRNRSL AA    PKNI +V+K
Sbjct: 241 SRCSRVNGRGWRCCQQTLVGYSLCEHHLGKGRLRSMNNVRNRSLVAA---TPKNIVVVEK 288

Query: 301 DEKKPSPFP--------PPPPTSFMLTSKKRMKLGMVKARSISSLLGQPDGMVVTPSPPP 360
           DEKKPSP P        PPPP+SF LTSKKRMKLG+VKARS+SSLLGQP+ M V  + P 
Sbjct: 301 DEKKPSPSPPPSPFPSSPPPPSSFSLTSKKRMKLGVVKARSMSSLLGQPEAMAV--ATPL 288

Query: 361 PPPRMLYDDNID 362
            PP  L+DDNID
Sbjct: 361 SPPSTLHDDNID 288

[Close]

BLAST of Lsi03G017440 vs. NCBI nr
Match: XP_038903168.1 (uncharacterized protein LOC120089834 [Benincasa hispida])

HSP 1 Score: 422.5 bits (1085), Expect = 3.5e-114
Identity = 245/366 (66.94%), Postives = 265/366 (72.40%), Query Frame = 0


Query: 1   MRIRKRQVPLPFSSLSPLPLSDPLLHQSPPVQLPPSSDPPNLHPFPSDNALVSNPNSNSR 60
           MRIRKRQ+PLPFSS     LSDPLL  SPPVQLPPSSDPPNLHPFPSDNA          
Sbjct: 1   MRIRKRQLPLPFSS-----LSDPLLRHSPPVQLPPSSDPPNLHPFPSDNA---------- 60

Query: 61  PFLSFDFDFGFYLSSFPLQNCTEDILEKNQKLQQHDSRETIVIGAETTNHGVLPQSPSSS 120
                                     + NQKL QHDSR+T VIGAET N+GVLP S SS 
Sbjct: 61  -------------------------SDNNQKLHQHDSRKTTVIGAETINNGVLPLSTSS- 120

Query: 121 QVLIVALSITYIVGFINLSVIGSSIDGGRWCEREKTFPLKKRRGSFERSSDDQDNDDEQD 180
                                 SS  GGRWCEREK FPLKKRRGSFERS++DQD++DE D
Sbjct: 121 ----------------------SSQGGGRWCEREKAFPLKKRRGSFERSNNDQDDNDEDD 180

Query: 181 DNKDDQVVTT-IVNSLSDEKKPMKTKVNRKYTL-SQKQSKLGLDLAGKKRSRGGALMEGS 240
           D +DDQVVTT  V  L DEKK MKTK+N+K TL SQKQ+KLGLDL GKKRSRGGALMEGS
Sbjct: 181 DEEDDQVVTTSTVKLLPDEKKTMKTKMNKKSTLSSQKQTKLGLDLVGKKRSRGGALMEGS 240

Query: 241 RCSRVNGRGWRCCQQTLVGYSLCEHHLGKGRLRSMNNVRNRSLAAAIATVPKNIAIVDKD 300
           RCSRVNGRGWRCCQQTLVGYSLCEHHLGKGRLRSMNNVR+RSLAAA AT PKN+ +  +D
Sbjct: 241 RCSRVNGRGWRCCQQTLVGYSLCEHHLGKGRLRSMNNVRSRSLAAATAT-PKNVVVDVED 300

Query: 301 EKK--PSPFPPPPPTSFMLTSKKRMKLGMVKARSISSLLGQPDGMVVTPSPPPPPPRMLY 360
           EKK  PSPFP PP +S  L SKKRMKLG+VKARSISSLLGQPDGMV TP PPPPPP M++
Sbjct: 301 EKKPSPSPFPAPPSSSLTLASKKRMKLGVVKARSISSLLGQPDGMVATPPPPPPPPPMVH 302

Query: 361 DDNIDD 363
           DDNIDD
Sbjct: 361 DDNIDD 302

[Close]

BLAST of Lsi03G017440 vs. NCBI nr
Match: XP_023526164.1 (uncharacterized protein LOC111789724 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 361.7 bits (927), Expect = 7.4e-96
Identity = 219/366 (59.84%), Postives = 236/366 (64.48%), Query Frame = 0


Query: 1   MRIRKRQVPLPFSSLSPLPLSDPLLHQSPPVQLP---PSSDPPNLHPFPSDNALVSNPNS 60
           MRIRKRQVPLPFSSLSPLPLSDPLL ++PPVQL    PSSDPPNLHP+  D+A       
Sbjct: 1   MRIRKRQVPLPFSSLSPLPLSDPLLRRTPPVQLHLQLPSSDPPNLHPYACDSAAYH---- 60

Query: 61  NSRPFLSFDFDFGFYLSSFPLQNCTEDILEKNQKLQQHDSRETIVIGAETTNHGVLPQSP 120
                                   T + L K  +L+QHDSRET V GAET+N GVLP S 
Sbjct: 61  ------------------------TNNALGKKDQLEQHDSRETTVFGAETSN-GVLPLSS 120

Query: 121 SSSQVLIVALSITYIVGFINLSVIGSSIDGGRWCEREKTFPLKKRRGSFERSSDDQDNDD 180
           SS QV                         G+WCER+K FPLKKRRGSFER SDD  N  
Sbjct: 121 SSPQVC-----------------------DGQWCERDKEFPLKKRRGSFERQSDDDQN-- 180

Query: 181 EQDDNKDDQVVTT--IVNSLSDEKKPMKTKVNRKYTLSQKQSKLGLDLAGKKRSRGGALM 240
               N DDQ  TT   + SL D KK  K K         KQ KLGL+LAGKKRSRGGALM
Sbjct: 181 ----NADDQTTTTAAAMESLPDHKKTTKNK---------KQPKLGLNLAGKKRSRGGALM 240

Query: 241 EGSRCSRVNGRGWRCCQQTLVGYSLCEHHLGKGRLRSMNNVRNRSLAAAIATVPKNIAIV 300
           EGSRCSRVNGRGWRCCQQTLVGYSLCEHHLGKGRLRSMNNVR+RSL+AA A   KNI  V
Sbjct: 241 EGSRCSRVNGRGWRCCQQTLVGYSLCEHHLGKGRLRSMNNVRSRSLSAAAAATLKNIVTV 299

Query: 301 DKDEKKPSPFPPPPPTSFMLTSKKRMKLGMVKARSISSLLGQPDGMVVTPSPPPPPPRML 360
           DKDEKKP P PPPP  S  L SKKR KLG+VKARSISSLLGQPD M   P PPPP    L
Sbjct: 301 DKDEKKPPPCPPPPSPSLTLASKKRTKLGLVKARSISSLLGQPDSMAAIPPPPPPSQSTL 299

Query: 361 YDDNID 362
            +DNI+
Sbjct: 361 PNDNIN 299

[Close]

BLAST of Lsi03G017440 vs. NCBI nr
Match: XP_022934290.1 (uncharacterized protein LOC111441497 [Cucurbita moschata])

HSP 1 Score: 357.8 bits (917), Expect = 1.1e-94
Identity = 217/364 (59.62%), Postives = 235/364 (64.56%), Query Frame = 0


Query: 1   MRIRKRQVPLPFSSLSPLPLSDPLLHQSPPVQLP---PSSDPPNLHPFPSDNALVSNPNS 60
           MRIRKRQVPLPFSSLSPLPLSDPLL ++PPVQL    PSSDPPNLHP+  D+A       
Sbjct: 1   MRIRKRQVPLPFSSLSPLPLSDPLLRRTPPVQLHLQLPSSDPPNLHPYACDSAAYH---- 60

Query: 61  NSRPFLSFDFDFGFYLSSFPLQNCTEDILEKNQKLQQHDSRETIVIGAETTNHGVLPQSP 120
                                   T + L K  +L+QHDSRET V GAE +N GVLP S 
Sbjct: 61  ------------------------TNNALGKKDQLEQHDSRETTVFGAEASN-GVLPLSS 120

Query: 121 SSSQVLIVALSITYIVGFINLSVIGSSIDGGRWCEREKTFPLKKRRGSFERSSDDQDNDD 180
           SS QV                         G+WCER+K FPLKKRRGSFER SDD  N+ 
Sbjct: 121 SSPQV-----------------------GDGQWCERDKDFPLKKRRGSFERQSDDDQNN- 180

Query: 181 EQDDNKDDQVVTTIVNSLSDEKKPMKTKVNRKYTLSQKQSKLGLDLAGKKRSRGGALMEG 240
             DD          + SL D KK  K K         KQ KLGL+LAGKKRSRGGALMEG
Sbjct: 181 -ADDQTTTAAAAAAMESLPDHKKTTKNK---------KQPKLGLNLAGKKRSRGGALMEG 240

Query: 241 SRCSRVNGRGWRCCQQTLVGYSLCEHHLGKGRLRSMNNVRNRSLAAAIATVPKNIAIVDK 300
           SRCSRVNGRGWRCCQQTLVGYSLCEHHLGKGRLRSMNNVR+RSLAAA AT+ KNI  VDK
Sbjct: 241 SRCSRVNGRGWRCCQQTLVGYSLCEHHLGKGRLRSMNNVRSRSLAAAAATL-KNIVTVDK 300

Query: 301 DEKKPSPFPPPPPTSFMLTSKKRMKLGMVKARSISSLLGQPDGMVVTPSPPPPPPRMLYD 360
           DEKKP P PPPP  S  L SKKR KLG+VKARSISSLLGQPDGM   P PPPP    L +
Sbjct: 301 DEKKPPPCPPPPSPSLTLASKKRTKLGLVKARSISSLLGQPDGMAAIPPPPPPSQSTLPN 300

Query: 361 DNID 362
           DNI+
Sbjct: 361 DNIN 300

[Close]

BLAST of Lsi03G017440 vs. NCBI nr
Match: KAG6580802.1 (hypothetical protein SDJN03_20804, partial [Cucurbita argyrosperma subsp. sororia] >KAG7017550.1 hypothetical protein SDJN02_19416 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 356.3 bits (913), Expect = 3.1e-94
Identity = 219/367 (59.67%), Postives = 235/367 (64.03%), Query Frame = 0


Query: 1   MRIRKRQVPLPFSSLSPLPLSDPLLHQSPPVQLP---PSSDPPNLHPFPSDNALVSNPNS 60
           MRIRKRQVPLPFSSLSPLPLSDPLL ++PPVQL    PSSDPPNLH +  D+A       
Sbjct: 1   MRIRKRQVPLPFSSLSPLPLSDPLLRRTPPVQLHLQLPSSDPPNLHTYACDSAAYH---- 60

Query: 61  NSRPFLSFDFDFGFYLSSFPLQNCTEDILEKNQKLQQHDSRETIVIGAETTNHGVLPQSP 120
                                   T + L K  +L+QHDSRET V GAET+N GVLP S 
Sbjct: 61  ------------------------TNNALGKKDQLEQHDSRETTVFGAETSN-GVLPLSS 120

Query: 121 SSSQVLIVALSITYIVGFINLSVIGSSIDGGRWCEREKTFPLKKRRGSFERSSDDQDNDD 180
           SS QV                         G+WCER+K FPLKKRRGSFER SDD  N  
Sbjct: 121 SSPQV-----------------------GDGQWCERDKEFPLKKRRGSFERQSDDDQN-- 180

Query: 181 EQDDNKDDQVVT---TIVNSLSDEKKPMKTKVNRKYTLSQKQSKLGLDLAGKKRSRGGAL 240
               N DDQ  T     + SL D KK  K K         KQ KLGL+LAGKKRSRGGAL
Sbjct: 181 ----NADDQTTTAAAAAMESLPDHKKTTKNK---------KQPKLGLNLAGKKRSRGGAL 240

Query: 241 MEGSRCSRVNGRGWRCCQQTLVGYSLCEHHLGKGRLRSMNNVRNRSLAAAIATVPKNIAI 300
           MEGSRCSRVNGRGWRCCQQTLVGYSLCEHHLGKGRLRSMNNVR+RSLAAA A   KNI  
Sbjct: 241 MEGSRCSRVNGRGWRCCQQTLVGYSLCEHHLGKGRLRSMNNVRSRSLAAAAAL--KNIVT 298

Query: 301 VDKDEKKPSPFPPPPPTSFMLTSKKRMKLGMVKARSISSLLGQPDGMVVTPSPPPPPPRM 360
           VDKDEKKP P PPPP  S  L SKKR KLG+VKARSISSLLGQPDGM   P PPPP    
Sbjct: 301 VDKDEKKPPPCPPPPSPSLTLASKKRTKLGLVKARSISSLLGQPDGMAAIPPPPPPSQST 298

Query: 361 LYDDNID 362
           L +DNI+
Sbjct: 361 LPNDNIN 298

[Close]

BLAST of Lsi03G017440 vs. NCBI nr
Match: XP_022983045.1 (uncharacterized protein LOC111481709 isoform X1 [Cucurbita maxima])

HSP 1 Score: 355.9 bits (912), Expect = 4.1e-94
Identity = 219/364 (60.16%), Postives = 237/364 (65.11%), Query Frame = 0


Query: 1   MRIRKRQVPLPFSSLSPLPLSDPLLHQSPPVQLP---PSSDPPNLHPFPSDNALVSNPNS 60
           MRIRKRQVPL FSSLSPLPLSDPLL ++PPVQL    PSSDPPNLHP+  D+A       
Sbjct: 1   MRIRKRQVPLSFSSLSPLPLSDPLLRRTPPVQLHLQLPSSDPPNLHPYACDSA------- 60

Query: 61  NSRPFLSFDFDFGFYLSSFPLQNCTEDILEKNQKLQQHDSRETIVIGAETTNHGVLPQSP 120
                                 + T + L K  +L+QHDSRET V GAET+N GVLP S 
Sbjct: 61  ---------------------ADHTNNALGKKDQLEQHDSRETTVFGAETSN-GVLPLSS 120

Query: 121 SSSQVLIVALSITYIVGFINLSVIGSSIDGGRWCEREKTFPLKKRRGSFERSSDDQDNDD 180
           SS QV                         G+WCER+K FPLKKRRGSFER SDD  N  
Sbjct: 121 SSPQV-----------------------GDGQWCERDKEFPLKKRRGSFERQSDDDQN-- 180

Query: 181 EQDDNKDDQVVTTIVNSLSDEKKPMKTKVNRKYTLSQKQSKLGLDLAGKKRSRGGALMEG 240
               N DDQ  TT + SL D KK  K K         KQ KLGL+LAGKKRSRGGALMEG
Sbjct: 181 ----NADDQ-TTTAMESLQDHKKTTKNK---------KQPKLGLNLAGKKRSRGGALMEG 240

Query: 241 SRCSRVNGRGWRCCQQTLVGYSLCEHHLGKGRLRSMNNVRNRSLAAAIATVPKNIAIVDK 300
           S CSRVNGRGWRCCQQTLVGYSLCEHHLGKGRLRSMNNVR+RSLAAA AT+ KNI  VDK
Sbjct: 241 SLCSRVNGRGWRCCQQTLVGYSLCEHHLGKGRLRSMNNVRSRSLAAAAATL-KNIVTVDK 295

Query: 301 DEKKPSPFPPPPPTSFMLTSKKRMKLGMVKARSISSLLGQPDGMVVTPSPPPPPPRMLYD 360
           DEKKP P PPPP  S  L SKKR KLG+VKARSISSLLGQPDGM   P  PPP    L +
Sbjct: 301 DEKKPPPCPPPPSPSLTLASKKRTKLGLVKARSISSLLGQPDGMAAIPPSPPPSQSTLPN 295

Query: 361 DNID 362
           DNI+
Sbjct: 361 DNIN 295

[Close]

BLAST of Lsi03G017440 vs. TAIR 10
Match: AT2G42040.1 (CONTAINS InterPro DOMAIN/s: WRC (InterPro:IPR014977); Has 219 Blast hits to 219 proteins in 19 species: Archae - 0; Bacteria - 0; Metazoa - 3; Fungi - 0; Plants - 215; Viruses - 0; Other Eukaryotes - 1 (source: NCBI BLink). )

HSP 1 Score: 120.6 bits (301), Expect = 2.7e-27
Identity = 115/340 (33.82%), Postives = 150/340 (44.12%), Query Frame = 0


Query: 1   MRIRKRQVPLPFSSLSPLPLSDPLLHQSPPVQLPPSSDPPNLHPFPSDNALVSNPNSNSR 60
           MRIRKRQVPLP SSL P+PLSD   ++SP                       +       
Sbjct: 1   MRIRKRQVPLPLSSLLPVPLSDLYFNRSP----------------------TATARYFRG 60

Query: 61  PFLSFDFDFGFYLSSFPLQNCTEDILEKNQKLQQHDSRETIVIGAETTNHGVLPQSPSSS 120
            +     DFG    S P  +   D L +   +++ +     V   +  N  V  +S + +
Sbjct: 61  GYKDGGDDFGSLQLSLPPPSQISDRLIQRDLIKKKEE----VKALDDDNGDVDVKSRTDA 120

Query: 121 QVLIVALSITYIVGFINLSVIGSSIDGGRWCER-EKTFPLKKRRGSFERSSDDQDNDDEQ 180
                        G  N++  G S+   +  E+ EK   L+KRRG F    D +D +DE+
Sbjct: 121 S------------GSKNVNPRGESVSSIQVVEKNEKVVSLRKRRG-FINFEDYEDEEDEE 180

Query: 181 DDNKDDQVVTTIVNSLSDEKKPMKTKVNRKYTLSQKQSKLGLDLAGKKRSR--GGALMEG 240
                                    ++N+                GKK+++  GG L EG
Sbjct: 181 ASGGGG-------------------RINK----------------GKKKAKKSGGGLEEG 240

Query: 241 SRCSRVNGRGWRCCQQTLVGYSLCEHHLGKGRLRSMNNVRNRSLAAAIATVPKNIAIVDK 300
           SRCSRVNGRGWRCCQQTLVGYSLCEHHLGKGR+RSMN       +       K   +V+ 
Sbjct: 241 SRCSRVNGRGWRCCQQTLVGYSLCEHHLGKGRVRSMNK------SGGGRGGEKKAVVVE- 241

Query: 301 DEKKPSPFPPPPPTSFMLTSKKRMKLGMVKARSISSLLGQ 338
                               KKR+KLGMVKARSISSLLGQ
Sbjct: 301 ------------------VKKKRVKLGMVKARSISSLLGQ 241

[Close]

BLAST of Lsi03G017440 vs. TAIR 10
Match: AT5G53660.1 (growth-regulating factor 7 )

HSP 1 Score: 44.7 bits (104), Expect = 1.9e-04
Identity = 17/42 (40.48%), Postives = 26/42 (61.90%), Query Frame = 0


Query: 235 MEGSRCSRVNGRGWRCCQQTLVGYSLCEHHLGKGRLRSMNNV 277
           +E  RC R +G+ WRC ++ +  +  CE HL +GR RS  +V
Sbjct: 108 LEPGRCRRTDGKKWRCAKEVVSNHKYCEKHLHRGRPRSRKHV 149

The following BLAST results are available for this feature:

BLAST of Lsi03G017440 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q6AWY1	9.1e-04	37.50	Growth-regulating factor 8 OS=Oryza sativa subsp. japonica OX=39947 GN=GRF8 PE=2...	[more]

BLAST of Lsi03G017440 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1F795	5.2e-95	59.62	Growth-regulating factor OS=Cucurbita moschata OX=3662 GN=LOC111441497 PE=3 SV=1	[more]
A0A6J1J4K8	2.0e-94	60.16	Growth-regulating factor OS=Cucurbita maxima OX=3661 GN=LOC111481709 PE=3 SV=1	[more]
A0A5A7TKD1	1.7e-90	59.68	Growth-regulating factor OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffol...	[more]
A0A1S3B737	1.7e-90	59.68	Growth-regulating factor OS=Cucumis melo OX=3656 GN=LOC103486754 PE=3 SV=1	[more]
A0A0A0LGX5	9.5e-89	58.87	Growth-regulating factor OS=Cucumis sativus OX=3659 GN=Csa_3G806230 PE=3 SV=1	[more]

BLAST of Lsi03G017440 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038903168.1	3.5e-114	66.94	uncharacterized protein LOC120089834 [Benincasa hispida]	[more]
XP_023526164.1	7.4e-96	59.84	uncharacterized protein LOC111789724 [Cucurbita pepo subsp. pepo]	[more]
XP_022934290.1	1.1e-94	59.62	uncharacterized protein LOC111441497 [Cucurbita moschata]	[more]
KAG6580802.1	3.1e-94	59.67	hypothetical protein SDJN03_20804, partial [Cucurbita argyrosperma subsp. sorori...	[more]
XP_022983045.1	4.1e-94	60.16	uncharacterized protein LOC111481709 isoform X1 [Cucurbita maxima]	[more]

BLAST of Lsi03G017440 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G42040.1	2.7e-27	33.82	CONTAINS InterPro DOMAIN/s: WRC (InterPro:IPR014977); Has 219 Blast hits to 219 ...	[more]
AT5G53660.1	1.9e-04	40.48	growth-regulating factor 7	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR014977	WRC domain	PFAM	PF08879	WRC	coord: 236..276 e-value: 6.6E-15 score: 54.5
IPR014977	WRC domain	PROSITE	PS51667	WRC	coord: 234..278 score: 21.18947
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 28..42
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 162..185
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 336..362
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 19..51
None	No IPR available	PANTHER	PTHR31602:SF19	OS02G0773600 PROTEIN	coord: 27..342
IPR031137	Growth-regulating factor	PANTHER	PTHR31602	FAMILY NOT NAMED	coord: 27..342

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi03G017440.1	Lsi03G017440.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0032502	developmental process
biological_process	GO:0006351	transcription, DNA-templated
cellular_component	GO:0005634	nucleus
molecular_function	GO:0005524	ATP binding