Lsi03G015110 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi03G015110
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	2-isopropylmalate synthase
Location	chr03: 26134735 .. 26142953 (+)
RNA-Seq Expression	Lsi03G015110
Synteny	Lsi03G015110

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CTGCAATATTTTGTGTCCACAATAATATTGGTGCCCACAAATAATTACTCCAACAGGAAGAAGGGGAGACCTCAAAAAATATTTTTCATTAGACTTTGCACTCCGAATGCCGATGTCGATGCCGATGCCAATGCCGATATTGATGCCGATAGTATCGAATAAGTATGTTAATATTAGTTGCCGTCCAATAACATCGTGTAGTGTGGTTTCTTCGATTCGCCAACGTCCACCCTATACACCAAATCACATCTCAGACACTTCCTACGTCCGCGTATTTGACACTACTCTACGCGACGGTGAGCAATCCCCAGGTGCAGCCCTGACGTCAAAGCAGAAGTTCCACATTGCTCGTGAGCTTGTGAAGCTCGGTGTTGACGCAATCGAGGCGGGATTCCCGGCAGCCTCAAGGCAGGACTTTGAGGCTGTGGAGATGATTGCGAAAGAGGTTGGGAATGTGGTCGATGAGGACGGGTACGTGCCAGTGATTTGTGGTTTCTCAAGGTGTACCGAGAGTGACGTTCGAGCTGCATGGGAGGCCGTCAAGTATGCTAAGAGACCAAGGATACATATGTTTATTGCAACAAGTGAAATTCATTTGAAGCATAAGCTTAGAAAGACAAGAGGGGAAGTGGTTGACATTGCTAGGAATATGGTGAGGTTTGCACAAAGTTTGGGTTGTCAAGATGTTGAGTTTAGCCCTGAGGATGCTGGCAGGTAATTTAATTAATTTCATCTTTAATCTCTACTTTGACTTCTTTTTCTTTTTTTTTTTTTCAAAAAAAAAGTCTTTATCTTCTAAAAACACTGACACCTGTCAAATACTAATCTATCGATTGCAAAAATAAATTACATAGCTCAAACTGATATAAAAGCTTATATTATCAACTTCGAAGTCAAAGGTTCGATTCTCCTACCTCATATGCTGTCTGAAAAAAGAAAAAAAAAACTCTAACTAATGACGTATCTATAAAGATCTTCTACTCATCATTAATTACTAACCAAAAAAACCAAACGTATGTTTTAGCTTTTATGACACCTTTTTTTTTCTCTCTCTTTTCCTTTAATTTGGTTTGCCTTTTGCATCATGAAACTTCGTTTTAATATGGTGCATATGAAAAAAATGAGAGAGGAAAACTTTTTTGGTTTTTTCAAAAAAAAAAAAATATACACAACTTTAATATTTCTAAGACTTTTTTTCATATTTATAAATAGTTTGATATTTTTTCCATTTTTAAAAATAACTCTAATCAAAATATCTTTTTTATAAAACCATTATAATATTTCTAAAAGAAATCATATTTATTTTAAGTTAAGAAAATAAAAATAAAAATAAAAGAAAATATTATTAATTAATATTAGAAATTAATTGAAAGAATAGTATTTTTTAGGGAGAGTTGCAAATATAGCAATCAAATTCAAAGTATTAGCATATATAGCAACATTTTAAAAAAAATTACAAATATAAAAAAGTCTATCAGTGATAGACTTCTATCCCTGATAGACTTTTATGGTCTAGGTGGTCTATCACGATTTATTGTTAACAGTGAAAATTTTCTATAATCGTAAATAGTTTGATATTTTTTTCTTTTATAATAATTTTCTATAATTAAATTATTTTTTAAAGTTTACAAAACACATGAAACACACATAGTATGAAATTAGTTTTTCTAAAACCACAACTGCCAACAATTATTTTTAAAGTTACAACAATCTCAAATTAATGAAATTTACTTTGATTCGTATAATTAAATTACCCAAATTTAATATTAACTTACTAAATACAGTGACTAAAATTTGTTTCTAGTTTAAAATTAAAATTTACTGAACGCTATTAATATTTTTTAATTTAAAATTAATTTACTCTGACATCATAACTAGTTTTCTTGTTTACTGTTCTTTAAACAATTAATACAAGTCATAGATTAATGACACCAAAAAATAGTCACAAGAGTTGAAAAATTAAAAAAAAAATCGTCTTTTCCCACCAAAATCGTCTGTTTAATTTTCAATTTAACGACTCTAAAAAATGTCACTTTTTAATAACGGTTAATTCTAGTCATAAATTGCTGACAAAAAAAATGTCAAAATAGTAAAACGGTCATAAATTTTAGAAAATTCAAAAAATTCAATTTTTTTCTACAAAAAAAATGCGTGATTTTGGCTTCAATGATGATTTTTCTCTCTCCTTCCTATTTTTACCATCGTTCATGTGTTAAAAAAAATCATATTTGTTGTAGTGACAAGTGAAAGCAGTATTTATAGGCTTAATTTTTAAAAACCAAAAAGCGAAAAAATATTATTGAAAAAATAACTAATGTCCCACTAAAACATATAATATACTTTAAATGTTAAAGTAATTTGTTTTATGGTGTTGTTATATATCAGATCTGATAAAGAATTCGTTTATGAAATTCTTGGTGAAGTCATAAGAGCTGGGGCAACTACTGTGAACATAACAGATACAGTGGGTTATACTGTGCCATTTGAATACGGGAACTTAATTGCTGATATAAAATCTAATACTCATGGCATTCATAATGTCATCATTTCAACACATTGCCAAAATGATCTTGGACTAGCAACTGCCAACACAATAGCAGTAAGTATACTAATATGCCTTCATTTTTTTTTTTTTTTTTTAATTAGAAGGAAAAAAAAAACTTAAATTTCTCTTTACCAAAAGTTCCCTGATTCTTTATGGGAAATTTCCCGTAATTTTTTTTTTTCTAATTTAAACTTAAGTTAAGTTAACCAATTTCAATTACTGTAAGCTTTAGTTAAATTCTTTTTTCAACCAATTTCAGTATTTTTTTTTTCCTATAAGTTTAAATTAATTAGTTTTCTTTTTTTTTTTTAAATTTAAGCTTCAGGGAATTTCCGTAAGAATTGGGATAGTTTAAGTTTTTTAATTACCATGATTAGTTTTGAGTAAAATTTTTTTATGAATTTCTCGTAAGAATTGAAATTTACGGAAAAATTTTCTAACTTTCTAATTCTTATAAGAAATTTCACGTGAGATTTTTTTTTTTTTTTTTTTTACTGGAAAATTTTCAATTCTAACGGAAAATAGTTTGTTGAAACTTTTTTCAATTTTTTTTGTTTTCAAATTATTACCGCTTGGAAATTATAATTTTTACCAAAAAATTTTTTGTAACGATTTCGAAAATTAACTTTTACAGTAAAAATTGGTTGAAATCTCGATAAAAAAAAAAATTGGTAAGAAACTATATAAAAAAAAAATTATTGTTAAAAACAACCTCTCTCCCAACTAAATTTCTTTTCCAAATTGGTTAGAAACTATAAAAGAAAGTTATGGTTAAAAACAACAAATCTATCGGTTGAAATTTGCCAAAAATATGGAGATAAATAAAATGAAAATTAAAGGATAAAATTGAAAACATTTAAATAAGAGACGAAATGATATTTTCATTATGTGTGGGATCCTTCACTTAAATCTAAAATAGTAAGAAAGGTGTTAGAAAAAGTAGTTTTTAAAAATAGATTAATTTTGAAGGATGAAAACTTTTTTAAATTAAAAATAGCGTAATTTTTCAAAAACAAACACAATTGAGCTAAAATATTATCAAATGGAGAGGATATTTTATAGACATGATGAGACTGCCATCATTGAATATATACTTTTATTTTCCACAAAAGAATATTCAAAGAGACTCGTAAATTAAAATAATATATCATCTAATGACATGTCAAAATATGAGTAATGAGTAAAATACTACGAAAGTGCTAGAAATTTTTTCTAATACATATACCTTTTTGTTTTCTCCCTTAAGTCTTGCTAGAAATTTCAAGGTACATCATGTTTGATTTTTGTCTCACTTTTTTAGGGGGCGTGTGGTGGTGCAAGGCAAGTTGAGGTCACGATAAATGGCATAGGTGAGAGAGCTGGAAATGCTTCGCTAGAGGAGGTAATTTATATATATATAAATTAAGGTTATTTTCAAATATAAAGTATTAGCTAAATTATTTACAAATATACAAAAATTTTACTATCTATAGCGATAGACTGCAATAGAATTTTATAGCTTGAGCGATAGATAGTAATAAAAATCTATCGCTCAAGCGATAACATTCTATCGCGGTATATCGTTGATAGATAGTAAAAATTTTCTATATTTATAAACAGTTTGATATTTTTTCTGTTTATAGTAATTTCTCAAAATATTATTGTATTCTTCTTTTTAAAAAAATTAATAATTTAAATTGGATGGTAAAAACAATGATACCAAACTTTGCAACCCACAAAGAATGTCCATTAATTAATCTCTATCGTATNAAAAAAAAAAAAAAGATGTAAGTAGATGCAATAATATATATAGATGTATATAAAAAAAAGAAGTAAAGAGTCTCAATTACAATTTATAATATAAAAAAAAACTATTTCAACGATAATTTTTATTTTTGGTTGTCAATGAAAAACTTAAAAATTAGCATTTTTTAATAGTTGATACCGACTTTATTGACGTTTAAAGAATGTAAGCTATAATTTTTATGAACTTTTTTTGAACGTCAACAAACACCCAAATTTGTATAAAAAAAAAAAAAAAAAAAAAAAAAAATCTATTCCTTCCTCAACAAAAGAACAATGAAATAATTTTGTTTTCTAAAATTTCATACCCTTTAATGGTTAGATAACAACAATCATATCTAATATCAAAATTAGTACAACTTAGAGCATTTTTTTGGGTAACGTATCTATCCTTTAGTTTTATAAGTTTATCTAAGTTGTATGGCTCGATAAAGCATCGATATACGTCTTTATCCATATATCTATTTTTGAATTGAATTAAAAAAATTTAATTAACATAAAATAGAACAACTTAATTAGAGCAAAACTATTACATATATATAGTGATGAATATAGGAGTGGATATTTTCAGGTGGTAATGACCCTACAATGTCGTGGAGATCATGTGCTTGGAGGTCTTTTCACTGGAATTAATTCAAGACATATCTTTTCGGCTAGCAAGATGGTAATTTTTATAATTTTAATTACGAAAAAAGTTATTAATTTTTTTTCTTTTTAACTTTTCTTAATTTTCTTTTAGCTATGAAATAATGGAGTGATAAATTAATTGAGTGTATATTATTAATTTGGTCAGGTAGAAGAGTACAGTGGGTTGAATGTACAGCCACACAAGGCCATTGTTGGAGCTAATGCTTTTGCTCATCAAAGTGGCATCCACCAGGTCACTCTTCTTCCATTTTTACTCGACGTATAAGCTTTCGTGGTTTTGTTTTAATTTCACATAAATTAAAAGAGCTTCGTATCATTGGAGATATAGTTATTATCACTTATATAACCAATATGAGACTTTGGTTGCGCTTCAACAATATTCTTCCCCTCGAACAAACGACAACCAAACGCCTACTCTCAAATAGTCATCCGTTAGTATTTACTCACGAGCAATCACTTTTAACCAAGCCAACTCCTCTTCACACTAGCAATCACTTTTCACAATGACGGTGTCATACCTTGATGCACACCTACACGTTTAGGGCATGCCTTTGATACCACTTGTATGTTAGGGTATAGCGTACCTTTATATATCTCTATAACTGTATGTTTTATTGTCCACTTTGGATATAAAATATATTCTTATGGCTTTGCTTTTGGAATCACCCAAAATGCATCGTACCAATGGAGATATATATTGTCTCATTCACTATTTATATACCTACGATATTCCCTTTTCTTAGCCAATGTAGAACTTTGATCGCACTCCAACAACCCTTCCTTTGAACGAAGGACCACCATAAATTAACCTCCCCTGAATCAAATAGTCATTCATTTTTGTTTTCTCTTGGGCAATTACTTTTAACTGGACCAACTCTTCTTTACCGGAGTCCATTCCATGTCCAATTAATTAGCTCAACCATCGATAATTGACTTTGTTATAGAGGTACACTGACATAACTTAATTTATGTCTAATAGATTCGTTAATCTTAAAAATGTTGAATATGTTTAGGACATATTAAATACAAATTTGGAAGTTCAAAGATTTATTTGACATTTTAAAATAAAAATATAGAGACAATTTAGACACAAAGTCACAAGTTCAAGACCTACAATAGATATTTTTATTAAAGTTTACGGACTTATTATATACCATTATGAAAATTTAGAGACCAAATTTATAATTAATTAAACTAATTAACTGTTGTTAGTACACATTTGCGAGGGGATGCAAGTGTTTGAGGCTCAAATTGAATGCTTAAATATGAATTTTGGTACAAACAGGATGGGATGCTTAAGCACAAAGGTACTTACGAAATCATTCATCCTGAAGAAATTGGCTATGAACGATCCAATCATGCTGGTATTGTTCTAGGAAAGCTTAGGTATGCTCTGTTTCTTTACTACAGTTAGTGATTTGTTTTATGAGAAAATAAATAATTTATTAGTGCTGGTAATTATTTTATTTTTAAATTTTCTTTTTATTTTCTATCAAACTTTCTTACTATGACTTTCATTTTTCTTAAAAAAAATATTTGAATTCTTAGTCAAAATCTAAAAACAAAAACAAGTTTTTTAAAATTATTCTTTTTTAGTTTTAGTTTTTAAAATTTGGTTTGATTTTTTACAACACTGGTGGAAAGTAAACTACACAACATACACATTTATGGGTAAAAGTAGATTTAGAAGCTTAATTTTCAAAACTAAAAATCACAAACAAAATGATTATCGAACAGGAATTAGAGAAAAGAAAAAAAATTAAACATGCCATGTATATAATTTTTAGAAGATGTGAAATATATATATGTATATTATAAAACTAGTTTTTAACATAAGAGATATTTTAATAATATTAAGAGAAAATATCAACTTATACCCCTAAATTTTAAAGCTTGCATCAATTAAGTTCCAAACTAATAATTGTATCAATTTAAACCTTAAACTTTTAATATATACCCTATACATTACGTTCTATTTGGAGAAACATCGTGTAACACTTATGAATAGTTTAAATTGACTCACTTATGACGATTTAGAAGTTTAATTAAAACAAAAAATATAAGTCCAACGCCATATTTTTCAAACGAATTAAAAAAAAAAGTTGTAAATTAATATACTTATAAAATGTTTAACGGTTAAACCGATACAATTATTATTTCCAAAATTACATTTTTTAAATATTTGGCTCGAGAGAAACAATATTTGGGGTGAGAGGTCTTAGAGGCTTTTGCTAGATTTTACTTATTTGTTTTTTTTTTTCAACGAAAGTTCAACCTTTGACTTTCTTATGTATATTTATTTTACAACATTTTACAGTGGACGTCATGCTCTTAAGAGTCAACTTCGAGAGGTAAATTGGGATCTCTCTATCCTTCTATATATTATGCTTTTCTCGTTGGCATATATATATATATATATATATGATAACACGTGGGTGGAGATCGAACCTTAGATACATCTTCATCTTTTCAGCTTGGTTATGAGATTGAAAATGACAACTTCGACAATATATTTTGTCACTTCAAAGCAATAGCTCAACAGAAAAAGGTAATTAAATATTTAATTTACAAATATATTTAGCTAATAGCGATTGAGATTCTACTTAAACTTATTAATTACTTAAAGAACACATAAATTACCATTCATCAATAAAGATTTTCATACTTTATTTTTTACATTTCAGGAAGTTACAAATGCAGATCTTCTAGCATTGATATCTGATGAAGTTTCTAAGAAAATTGATATTAATAGGTGACTGAAAAACAAACGTGGTCGGCATGAAATGTATGGATTAATTAATTTTTTTTATTTCTTTTTTTAAGAAAATTCTTGAACAAAAAGTGTGGATGTACAAAAATAATTTGTATATATTTGGCTTTATTAAAATTCTAATATTAGTATTTTTTTAATGATAGTTTTTACATGGAAACTCTTACAAAAATAGATTTCACAGCTCTGGATATTAATAGAAATAATTATTTGAAACAGGTGTTTGATGCTGAGATCCATTTAGATGTCATGGATCTTTGAAAAACAATTAAAGAAAGAAATATAATGTCAAGTCAAGAAAAGGTAAAAACCATGATATTTCTTCGCTGTCATATTCATGAGGGATTAAAATTTGAATACTTTTGTTCGCACAAGGTTTCCGGAGAAACGTGTTTCATGGAGTTGAACTTGTGTTTATATAAGTTTGATGCGGATGCGGTTCGATCTCTAGTACTTGATCCTCTGATTCTCTCT

mRNA sequence

CTGCAATATTTTGTGTCCACAATAATATTGGTGCCCACAAATAATTACTCCAACAGGAAGAAGGGGAGACCTCAAAAAATATTTTTCATTAGACTTTGCACTCCGAATGCCGATGTCGATGCCGATGCCAATGCCGATATTGATGCCGATAGTATCGAATAAGTATGTTAATATTAGTTGCCGTCCAATAACATCGTGTAGTGTGGTTTCTTCGATTCGCCAACGTCCACCCTATACACCAAATCACATCTCAGACACTTCCTACGTCCGCGTATTTGACACTACTCTACGCGACGGTGAGCAATCCCCAGGTGCAGCCCTGACGTCAAAGCAGAAGTTCCACATTGCTCGTGAGCTTGTGAAGCTCGGTGTTGACGCAATCGAGGCGGGATTCCCGGCAGCCTCAAGGCAGGACTTTGAGGCTGTGGAGATGATTGCGAAAGAGGTTGGGAATGTGGTCGATGAGGACGGGTACGTGCCAGTGATTTGTGGTTTCTCAAGGTGTACCGAGAGTGACGTTCGAGCTGCATGGGAGGCCGTCAAGTATGCTAAGAGACCAAGGATACATATGTTTATTGCAACAAGTGAAATTCATTTGAAGCATAAGCTTAGAAAGACAAGAGGGGAAGTGGTTGACATTGCTAGGAATATGGTGAGGTTTGCACAAAGTTTGGGTTGTCAAGATGTTGAGTTTAGCCCTGAGGATGCTGGCAGATCTGATAAAGAATTCGTTTATGAAATTCTTGGTGAAGTCATAAGAGCTGGGGCAACTACTGTGAACATAACAGATACAGTGGGTTATACTGTGCCATTTGAATACGGGAACTTAATTGCTGATATAAAATCTAATACTCATGGCATTCATAATGTCATCATTTCAACACATTGCCAAAATGATCTTGGACTAGCAACTGCCAACACAATAGCAGTGGTAATGACCCTACAATGTCGTGGAGATCATGTGCTTGGAGGTCTTTTCACTGGAATTAATTCAAGACATATCTTTTCGGCTAGCAAGATGGATGGGATGCTTAAGCACAAAGGTACTTACGAAATCATTCATCCTGAAGAAATTGGCTATGAACGATCCAATCATGCTGGTATTGTTCTAGGAAAGCTTAGTGGACGTCATGCTCTTAAGAGTCAACTTCGAGAGCTTGGTTATGAGATTGAAAATGACAACTTCGACAATATATTTTGTCACTTCAAAGCAATAGCTCAACAGAAAAAGGAAGTTACAAATGCAGATCTTCTAGCATTGATATCTGATGAAGTTTCTAAGAAAATTGATATTAATAGGTGACTGAAAAACAAACGTGGTCGGCATGAAATGTGTTTGATGCTGAGATCCATTTAGATGTCATGGATCTTTGAAAAACAATTAAAGAAAGAAATATAATGTCAAGTCAAGAAAAGGTAAAAACCATGATATTTCTTCGCTGTCATATTCATGAGGGATTAAAATTTGAATACTTTTGTTCGCACAAGGTTTCCGGAGAAACGTGTTTCATGGAGTTGAACTTGTGTTTATATAAGTTTGATGCGGATGCGGTTCGATCTCTAGTACTTGATCCTCTGATTCTCTCT

Coding sequence (CDS)

ATGCCGATGTCGATGCCGATGCCAATGCCGATATTGATGCCGATAGTATCGAATAAGTATGTTAATATTAGTTGCCGTCCAATAACATCGTGTAGTGTGGTTTCTTCGATTCGCCAACGTCCACCCTATACACCAAATCACATCTCAGACACTTCCTACGTCCGCGTATTTGACACTACTCTACGCGACGGTGAGCAATCCCCAGGTGCAGCCCTGACGTCAAAGCAGAAGTTCCACATTGCTCGTGAGCTTGTGAAGCTCGGTGTTGACGCAATCGAGGCGGGATTCCCGGCAGCCTCAAGGCAGGACTTTGAGGCTGTGGAGATGATTGCGAAAGAGGTTGGGAATGTGGTCGATGAGGACGGGTACGTGCCAGTGATTTGTGGTTTCTCAAGGTGTACCGAGAGTGACGTTCGAGCTGCATGGGAGGCCGTCAAGTATGCTAAGAGACCAAGGATACATATGTTTATTGCAACAAGTGAAATTCATTTGAAGCATAAGCTTAGAAAGACAAGAGGGGAAGTGGTTGACATTGCTAGGAATATGGTGAGGTTTGCACAAAGTTTGGGTTGTCAAGATGTTGAGTTTAGCCCTGAGGATGCTGGCAGATCTGATAAAGAATTCGTTTATGAAATTCTTGGTGAAGTCATAAGAGCTGGGGCAACTACTGTGAACATAACAGATACAGTGGGTTATACTGTGCCATTTGAATACGGGAACTTAATTGCTGATATAAAATCTAATACTCATGGCATTCATAATGTCATCATTTCAACACATTGCCAAAATGATCTTGGACTAGCAACTGCCAACACAATAGCAGTGGTAATGACCCTACAATGTCGTGGAGATCATGTGCTTGGAGGTCTTTTCACTGGAATTAATTCAAGACATATCTTTTCGGCTAGCAAGATGGATGGGATGCTTAAGCACAAAGGTACTTACGAAATCATTCATCCTGAAGAAATTGGCTATGAACGATCCAATCATGCTGGTATTGTTCTAGGAAAGCTTAGTGGACGTCATGCTCTTAAGAGTCAACTTCGAGAGCTTGGTTATGAGATTGAAAATGACAACTTCGACAATATATTTTGTCACTTCAAAGCAATAGCTCAACAGAAAAAGGAAGTTACAAATGCAGATCTTCTAGCATTGATATCTGATGAAGTTTCTAAGAAAATTGATATTAATAGGTGA

Protein sequence

MPMSMPMPMPILMPIVSNKYVNISCRPITSCSVVSSIRQRPPYTPNHISDTSYVRVFDTTLRDGEQSPGAALTSKQKFHIARELVKLGVDAIEAGFPAASRQDFEAVEMIAKEVGNVVDEDGYVPVICGFSRCTESDVRAAWEAVKYAKRPRIHMFIATSEIHLKHKLRKTRGEVVDIARNMVRFAQSLGCQDVEFSPEDAGRSDKEFVYEILGEVIRAGATTVNITDTVGYTVPFEYGNLIADIKSNTHGIHNVIISTHCQNDLGLATANTIAVVMTLQCRGDHVLGGLFTGINSRHIFSASKMDGMLKHKGTYEIIHPEEIGYERSNHAGIVLGKLSGRHALKSQLRELGYEIENDNFDNIFCHFKAIAQQKKEVTNADLLALISDEVSKKIDINR

Homology

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BLAST of Lsi03G015110 vs. ExPASy Swiss-Prot
Match: Q9C550 (2-isopropylmalate synthase 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=IPMS2 PE=1 SV=1)

HSP 1 Score: 518.1 bits (1333), Expect = 8.9e-146
Identity = 263/409 (64.30%), Postives = 310/409 (75.79%), Query Frame = 0


Query: 38  RQRPPYTPNHISDTSYVRVFDTTLRDGEQSPGAALTSKQKFHIARELVKLGVDAIEAGFP 97
           R+RP Y PN ISD +YVR+FDTTLRDGEQSPGA LTSK+K  IAR+L KLGVD IEAGFP
Sbjct: 71  RRRPNYIPNRISDPNYVRIFDTTLRDGEQSPGATLTSKEKLDIARQLAKLGVDIIEAGFP 130

Query: 98  AASRQDFEAVEMIAKEVGNVVDEDGYVPVICGFSRCTESDVRAAWEAVKYAKRPRIHMFI 157
           AAS+ DFEAV+ IA+ VGN VDE+GYVPVICG SRC + D+  AWEAVKYAKRPRIH FI
Sbjct: 131 AASKDDFEAVKTIAETVGNTVDENGYVPVICGLSRCNKKDIETAWEAVKYAKRPRIHTFI 190

Query: 158 ATSEIHLKHKLRKTRGEVVDIARNMVRFAQSLGCQDVEFSPEDAGRSDKEFVYEILGEVI 217
           ATS+IHLK+KL+K++ EV++IARNMVRFA+SLGC+DVEFSPEDAGRS++E++YEILGEVI
Sbjct: 191 ATSDIHLKYKLKKSKEEVIEIARNMVRFARSLGCEDVEFSPEDAGRSEREYLYEILGEVI 250

Query: 218 RAGATTVNITDTVGYTVPFEYGNLIADIKSNTHGIHNVIISTHCQNDLGLATANTIA--- 277
           +AGATT+NI DTVG T+P E+G LIADIK+NT GI NVIISTHCQNDLGL+TANT++   
Sbjct: 251 KAGATTLNIPDTVGITLPSEFGQLIADIKANTPGIQNVIISTHCQNDLGLSTANTLSGAH 310

Query: 278 ------------------------VVMTLQCRGDHVLGGLFTGINSRHIFSASKM----- 337
                                   VVM ++CRGDHVLGGLFTGI++RHI   SKM     
Sbjct: 311 SGARQVEVTINGIGERAGNASLEEVVMAIKCRGDHVLGGLFTGIDTRHIVMTSKMVEEYT 370

Query: 338 ------------------------DGMLKHKGTYEIIHPEEIGYERSNHAGIVLGKLSGR 391
                                   DGMLKHKGTYEI+ PEEIG ERSN AGIVLGKLSGR
Sbjct: 371 GMQTQPHKAIVGANAFAHESGIHQDGMLKHKGTYEIMSPEEIGLERSNDAGIVLGKLSGR 430

[Close]

BLAST of Lsi03G015110 vs. ExPASy Swiss-Prot
Match: Q9LPR4 (2-isopropylmalate synthase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=IPMS1 PE=1 SV=2)

HSP 1 Score: 508.1 bits (1307), Expect = 9.3e-143
Identity = 268/454 (59.03%), Postives = 324/454 (71.37%), Query Frame = 0


Query: 4   SMPMPMPILMPIVSNKYVNISCRPIT---SCSVV--------SSIRQRPPYTPNHISDTS 63
           S P P+        + + +IS R  T   SCS+         +  R RP Y PN ISD +
Sbjct: 28  SKPTPISSSFRFQPSHHRSISLRSQTLRLSCSISDPSPLPPHTPRRPRPEYIPNRISDPN 87

Query: 64  YVRVFDTTLRDGEQSPGAALTSKQKFHIARELVKLGVDAIEAGFPAASRQDFEAVEMIAK 123
           YVRVFDTTLRDGEQSPGA LTSK+K  IAR+L KLGVD IEAGFPAAS+ DFEAV+ IA+
Sbjct: 88  YVRVFDTTLRDGEQSPGATLTSKEKLDIARQLAKLGVDIIEAGFPAASKDDFEAVKTIAE 147

Query: 124 EVGNVVDEDGYVPVICGFSRCTESDVRAAWEAVKYAKRPRIHMFIATSEIHLKHKLRKTR 183
            VGN VDE+GYVPVICG SRC + D+  AW+AVKYAKRPRIH FIATS+IHL++KL+KT+
Sbjct: 148 TVGNTVDENGYVPVICGLSRCNKKDIERAWDAVKYAKRPRIHTFIATSDIHLEYKLKKTK 207

Query: 184 GEVVDIARNMVRFAQSLGCQDVEFSPEDAGRSDKEFVYEILGEVIRAGATTVNITDTVGY 243
            EV++IAR+MVRFA+SLGC+DVEFSPEDAGRS++E++YEILGEVI+AGATT+NI DTVG 
Sbjct: 208 AEVIEIARSMVRFARSLGCEDVEFSPEDAGRSEREYLYEILGEVIKAGATTLNIPDTVGI 267

Query: 244 TVPFEYGNLIADIKSNTHGIHNVIISTHCQNDLGLATANTIA------------------ 303
           T+P E+G LI D+K+NT GI NV+ISTHCQNDLGL+TANT++                  
Sbjct: 268 TLPSEFGQLITDLKANTPGIENVVISTHCQNDLGLSTANTLSGAHAGARQMEVTINGIGE 327

Query: 304 ---------VVMTLQCRGDHVLGGLFTGINSRHIFSASKM-------------------- 363
                    VVM ++CRGDHVLGGLFTGI++RHI   SKM                    
Sbjct: 328 RAGNASLEEVVMAIKCRGDHVLGGLFTGIDTRHIVMTSKMVEEYTGMQTQPHKAIVGANA 387

Query: 364 ---------DGMLKHKGTYEIIHPEEIGYERSNHAGIVLGKLSGRHALKSQLRELGYEIE 391
                    DGMLKHKGTYEII PEEIG ERSN AGIVLGKLSGRHALK +L ELGY+++
Sbjct: 388 FAHESGIHQDGMLKHKGTYEIICPEEIGLERSNDAGIVLGKLSGRHALKDRLTELGYQLD 447

[Close]

BLAST of Lsi03G015110 vs. ExPASy Swiss-Prot
Match: O04973 (2-isopropylmalate synthase A OS=Solanum pennellii OX=28526 GN=IPMSA PE=2 SV=1)

HSP 1 Score: 485.0 bits (1247), Expect = 8.4e-136
Identity = 251/439 (57.18%), Postives = 321/439 (73.12%), Query Frame = 0


Query: 9   MPILMPIVSNKYVNISCRPITSCSVVSSI-RQRPPYTPNHISDTSYVRVFDTTLRDGEQS 68
           + +L+PI+S   V  S +   S  +  SI  +RP Y P+ ISD  YVR+FDTTLRDGEQS
Sbjct: 5   LQLLVPIIS---VFQSKKHYYSTFIRCSISNRRPEYVPSKISDPKYVRIFDTTLRDGEQS 64

Query: 69  PGAALTSKQKFHIARELVKLGVDAIEAGFPAASRQDFEAVEMIAKEVGNVVDEDGYVPVI 128
           PGA +T+K+K  +AR+L KLGVD IEAGFPA+S  DFE+V++IA+E+GN  DE+G+VPVI
Sbjct: 65  PGATMTTKEKLDVARQLAKLGVDIIEAGFPASSEADFESVKLIAEEIGNNTDENGFVPVI 124

Query: 129 CGFSRCTESDVRAAWEAVKYAKRPRIHMFIATSEIHLKHKLRKTRGEVVDIARNMVRFAQ 188
           CG SRC +SD+  AWEAVKYAK+PR+H FIATSEIH+K+KL+ +R +VV+ AR+MV +A+
Sbjct: 125 CGLSRCNKSDIDKAWEAVKYAKKPRVHTFIATSEIHMKYKLKMSREQVVEKARSMVAYAR 184

Query: 189 SLGCQDVEFSPEDAGRSDKEFVYEILGEVIRAGATTVNITDTVGYTVPFEYGNLIADIKS 248
           SLGC+DVEFSPEDAGRSD+EF+Y+ILGEVI+AGATT+NI DTVGYTVP E+G LI DIK+
Sbjct: 185 SLGCEDVEFSPEDAGRSDREFLYDILGEVIKAGATTLNIPDTVGYTVPSEFGQLITDIKA 244

Query: 249 NTHGIHNVIISTHCQNDLGLATANTIA---------------------------VVMTLQ 308
           NT GI NVIISTHCQNDLGL+TANT+A                           VVM L+
Sbjct: 245 NTPGIENVIISTHCQNDLGLSTANTLAGACAGARQLEVTINGIGERAGNASLEEVVMALK 304

Query: 309 CRGDHVLGGLFTGINSRHIFSASKM-----------------------------DGMLKH 368
           CRG+ VLGGL+TGIN++HI  +SKM                             DGMLKH
Sbjct: 305 CRGEQVLGGLYTGINTQHIVPSSKMVEEYSGLQVQPHKAIVGANAFAHESGIHQDGMLKH 364

Query: 369 KGTYEIIHPEEIGYERSNHAGIVLGKLSGRHALKSQLRELGYEIENDNFDNIFCHFKAIA 391
           K TYEII P+++G  RSN AGIVLGKLSGRHALKS++ ELGY+I+    +++F  FK++A
Sbjct: 365 KDTYEIISPDDVGLSRSNEAGIVLGKLSGRHALKSKMLELGYDIDGKELEDLFWRFKSVA 424

[Close]

BLAST of Lsi03G015110 vs. ExPASy Swiss-Prot
Match: O04974 (2-isopropylmalate synthase B OS=Solanum pennellii OX=28526 GN=IPMSB PE=2 SV=1)

HSP 1 Score: 466.5 bits (1199), Expect = 3.1e-130
Identity = 239/420 (56.90%), Postives = 300/420 (71.43%), Query Frame = 0


Query: 27  PITSCSVVSSIRQRPPYTPNHISDTSYVRVFDTTLRDGEQSPGAALTSKQKFHIARELVK 86
           P+  C    SIR+ P YTP+HI D +YVR+FDTTLRDGEQSPGA +T+K+K  +AR+  K
Sbjct: 48  PVVRC----SIRRIPEYTPSHIPDPNYVRIFDTTLRDGEQSPGATMTTKEKLDVARQSAK 107

Query: 87  LGVDAIEAGFPAASRQDFEAVEMIAKEVGNVVDEDGYVPVICGFSRCTESDVRAAWEAVK 146
           LGVD IEAGFPA+S  D EAV++IAKEVGN V E+ YVPVICG +RC + D+  AWEAVK
Sbjct: 108 LGVDIIEAGFPASSEADLEAVKLIAKEVGNGVYEEEYVPVICGLARCNKKDIDKAWEAVK 167

Query: 147 YAKRPRIHMFIATSEIHLKHKLRKTRGEVVDIARNMVRFAQSLGCQDVEFSPEDAGRSDK 206
           YAK+PRIH FIATSE+H+ +KL+ +R +VV+ AR+MV +A+S+GC+DVEFSPEDAGRSD 
Sbjct: 168 YAKKPRIHTFIATSEVHMNYKLKMSRDQVVEKARSMVAYARSIGCEDVEFSPEDAGRSDP 227

Query: 207 EFVYEILGEVIRAGATTVNITDTVGYTVPFEYGNLIADIKSNTHGIHNVIISTHCQNDLG 266
           EF+Y ILGEVI+AGATT+NI DTVGYTVP E+G LIA IK+NT G+ +VIISTHCQNDLG
Sbjct: 228 EFLYHILGEVIKAGATTLNIPDTVGYTVPEEFGQLIAKIKANTPGVEDVIISTHCQNDLG 287

Query: 267 LATANTIA---------------------------VVMTLQCRGDHVLGGLFTGINSRHI 326
           L+TANT+A                           VVM L+CRG+ VLGGL+TGIN++HI
Sbjct: 288 LSTANTLAGACAGARQLEVTINGIGERAGNASLEEVVMALKCRGEQVLGGLYTGINTQHI 347

Query: 327 FSASKM-----------------------------DGMLKHKGTYEIIHPEEIGYERSNH 386
             +SKM                             DGMLKHK TYEII PE+IG  R+N 
Sbjct: 348 LMSSKMVEGISGLHVQPHKAIVGANAFVHESGIHQDGMLKHKDTYEIISPEDIGLNRANE 407

Query: 387 AGIVLGKLSGRHALKSQLRELGYEIENDNFDNIFCHFKAIAQQKKEVTNADLLALISDEV 391
           +GIV GKLSG    K ++ ELGYEIE    D++F  FK++A++KK++T+ DL+AL+SDEV
Sbjct: 408 SGIVFGKLSGVMLCKPKMLELGYEIEGKELDDLFWRFKSVAEKKKKITDDDLVALMSDEV 463

[Close]

BLAST of Lsi03G015110 vs. ExPASy Swiss-Prot
Match: Q39891 (Probable 2-isopropylmalate synthase OS=Glycine max OX=3847 GN=GMN56 PE=2 SV=1)

HSP 1 Score: 453.0 bits (1164), Expect = 3.5e-126
Identity = 234/404 (57.92%), Postives = 279/404 (69.06%), Query Frame = 0


Query: 40  RPPYTPNHISDTSYVRVFDTTLRDGEQSPGAALTSKQKFHIARELVKLGVDAIEAGFPAA 99
           RP Y PNHI D+SYVR+ DTTLRDGEQSPGA +T+K+K  IAR+LVKLGVD I+ GFP+A
Sbjct: 19  RPQYIPNHIPDSSYVRILDTTLRDGEQSPGATMTAKEKLDIARQLVKLGVDIIQPGFPSA 78

Query: 100 SRQDFEAVEMIAKEVGNVVDEDGYVPVICGFSRCTESDVRAAWEAVKYAKRPRIHMFIAT 159
           S  DF AV+MIA+EVGN VD+DGYVPVI GF RC E D+  AWEAVKYAKRPR+   IAT
Sbjct: 79  SNSDFMAVKMIAQEVGNAVDDDGYVPVIAGFCRCVEKDISTAWEAVKYAKRPRLCTSIAT 138

Query: 160 SEIHLKHKLRKTRGEVVDIARNMVRFAQSLGCQDVEFSPEDAGRSDKEFVYEILGEVIRA 219
           S IH++HKLRK++ +V+ IAR+MV+FA+SLGC D++F  EDA RSD+EF+YEILG VI A
Sbjct: 139 SPIHMEHKLRKSKDQVIQIARDMVKFARSLGCNDIQFGAEDATRSDREFLYEILGVVIEA 198

Query: 220 GATTVNITDTVGYTVPFEYGNLIADIKSNTHGIHNVIISTHCQNDLGLATANTI------ 279
           GATTVNI DTVG  +P E G LI DIK NT GI NVIISTHC NDLGLATANTI      
Sbjct: 199 GATTVNIADTVGIVMPLELGKLIVDIKDNTPGIANVIISTHCHNDLGLATANTIEGARTG 258

Query: 280 ---------------------AVVMTLQCRGDHVLGGLFTGINSRHIFSASKM------- 339
                                 VVM L  +GDH L GL+T IN+RHI   SKM       
Sbjct: 259 ARQLEVTINGIGERAGNASLEEVVMALASKGDHALNGLYTRINTRHILETSKMVEEYSGM 318

Query: 340 ----------------------DGMLKHKGTYEIIHPEEIGYERSNHAGIVLGKLSGRHA 388
                                 DGMLKHKGTYE I PEEIG++R+   GIVLGKLSG  A
Sbjct: 319 HLQPHKPLVGANAFVHASGIHQDGMLKHKGTYETISPEEIGHKRTTRIGIVLGKLSGSQA 378

[Close]

BLAST of Lsi03G015110 vs. ExPASy TrEMBL
Match: A0A6J1F3I8 (2-isopropylmalate synthase 2, chloroplastic-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111441807 PE=3 SV=1)

HSP 1 Score: 593.2 bits (1528), Expect = 8.1e-166
Identity = 319/452 (70.58%), Postives = 345/452 (76.33%), Query Frame = 0


Query: 1   MPMSMPMPMPILMPIVSNKYVNISCRPITSCSVVSSIRQRPPYTPNHISDTSYVRVFDTT 60
           MP+SM M M        N  V IS   ITSC VVSS+RQRPPYTPN I D  YVRVFDTT
Sbjct: 6   MPVSMSMSMS-----KPNHDVCISRHQITSCHVVSSVRQRPPYTPNSILDAPYVRVFDTT 65

Query: 61  LRDGEQSPGAALTSKQKFHIARELVKLGVDAIEAGFPAASRQDFEAVEMIAKEVGNVVDE 120
           LRDGEQSPG ALT KQK  IAR+LVKL VDAIEAGFPAASRQDFEAVEM+AKEVGN V E
Sbjct: 66  LRDGEQSPGTALTLKQKLLIARQLVKLRVDAIEAGFPAASRQDFEAVEMVAKEVGNEVGE 125

Query: 121 DGYVPVICGFSRCTESDVRAAWEAVKYAKRPRIHMFIATSEIHLKHKLRKTRGEVVDIAR 180
           DGYVPVICGFSRCTESDVRAAWEAVKYAKRPRIHMFIATSEIHL HKLRKTRGEVV+IAR
Sbjct: 126 DGYVPVICGFSRCTESDVRAAWEAVKYAKRPRIHMFIATSEIHLTHKLRKTRGEVVEIAR 185

Query: 181 NMVRFAQSLGCQDVEFSPEDAGRSDKEFVYEILGEVIRAGATTVNITDTVGYTVPFEYGN 240
           NMVRFA+SLG  DVEFSPEDAGRSDKEF+Y+ILGEVIRAGATT+NITDTVGYTVP EYG 
Sbjct: 186 NMVRFARSLGFDDVEFSPEDAGRSDKEFLYQILGEVIRAGATTLNITDTVGYTVPAEYGK 245

Query: 241 LIADIKSNTHGIHNVIISTHCQNDLGLATANTIA-------------------------- 300
           LIADIK NT G  NVIISTHCQNDLGLATANTIA                          
Sbjct: 246 LIADIKCNTPGTQNVIISTHCQNDLGLATANTIAGACGGARQVEVTINGIGEKAGNASLE 305

Query: 301 -VVMTLQCRGDHVLGGLFTGINSRHIFSASKM---------------------------- 360
            +VMTL+CRG+HV GGL+TGINSRHIFSASK+                            
Sbjct: 306 EMVMTLRCRGEHVFGGLYTGINSRHIFSASKIVEEYTGLNVQPHKAIVGANAFAHQSGIH 365

Query: 361 -DGMLKHKGTYEIIHPEEIGYERSNHAGIVLGKLSGRHALKSQLRELGYEIENDNFDNIF 397
            DGMLKH+GTYEII PE+IGY+RSNH  IVLGKLSGRHALKS LR+LGYEI+ D  DN+F
Sbjct: 366 QDGMLKHRGTYEIISPEDIGYQRSNHVVIVLGKLSGRHALKSYLRKLGYEIDEDKLDNVF 425

[Close]

BLAST of Lsi03G015110 vs. ExPASy TrEMBL
Match: A0A6J1F8D9 (2-isopropylmalate synthase 2, chloroplastic-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111441807 PE=3 SV=1)

HSP 1 Score: 567.8 bits (1462), Expect = 3.6e-158
Identity = 305/433 (70.44%), Postives = 328/433 (75.75%), Query Frame = 0


Query: 1   MPMSMPMPMPILMPIVSNKYVNISCRPITSCSVVSSIRQRPPYTPNHISDTSYVRVFDTT 60
           MP+SM M M        N  V IS   ITSC VVSS+RQRPPYTPN I D  YVRVFDTT
Sbjct: 6   MPVSMSMSMS-----KPNHDVCISRHQITSCHVVSSVRQRPPYTPNSILDAPYVRVFDTT 65

Query: 61  LRDGEQSPGAALTSKQKFHIARELVKLGVDAIEAGFPAASRQDFEAVEMIAKEVGNVVDE 120
           LRDGEQSPG ALT KQK  IAR+LVKL VDAIEAGFPAASRQDFEAVEM+AKEVGN V E
Sbjct: 66  LRDGEQSPGTALTLKQKLLIARQLVKLRVDAIEAGFPAASRQDFEAVEMVAKEVGNEVGE 125

Query: 121 DGYVPVICGFSRCTESDVRAAWEAVKYAKRPRIHMFIATSEIHLKHKLRKTRGEVVDIAR 180
           DGYVPVICGFSRCTESDVRAAWEAVKYAKRPRIHMFIATSEIHL HKLRKTRGEVV+IAR
Sbjct: 126 DGYVPVICGFSRCTESDVRAAWEAVKYAKRPRIHMFIATSEIHLTHKLRKTRGEVVEIAR 185

Query: 181 NMVRFAQSLGCQDVEFSPEDAGRSDKEFVYEILGEVIRAGATTVNITDTVGYTVPFEYGN 240
           NMVRFA+SLG  DVEFSPEDAGRSDKEF+Y+ILGEVIRAGATT+NITDTVGYTVP EYG 
Sbjct: 186 NMVRFARSLGFDDVEFSPEDAGRSDKEFLYQILGEVIRAGATTLNITDTVGYTVPAEYGK 245

Query: 241 LIADIKSNTHGIHNVIISTHCQNDLGLATANTIA-------------------------- 300
           LIADIK NT G  NVIISTHCQNDLGLATANTIA                          
Sbjct: 246 LIADIKCNTPGTQNVIISTHCQNDLGLATANTIAGACGGARQVEVTINGIGEKAGNASLE 305

Query: 301 -VVMTLQCRGDHVLGGLFTGINSRHIFSASKM---------------------------- 360
            +VMTL+CRG+HV GGL+TGINSRHIFSASK+                            
Sbjct: 306 EMVMTLRCRGEHVFGGLYTGINSRHIFSASKIVEEYTGLNVQPHKAIVGANAFAHQSGIH 365

Query: 361 -DGMLKHKGTYEIIHPEEIGYERSNHAGIVLGKLSGRHALKSQLRELGYEIENDNFDNIF 378
            DGMLKH+GTYEII PE+IGY+RSNH  IVLGKLSGRHALKS LR+LGYEI+ D  DN+F
Sbjct: 366 QDGMLKHRGTYEIISPEDIGYQRSNHVVIVLGKLSGRHALKSYLRKLGYEIDEDKLDNVF 425

[Close]

BLAST of Lsi03G015110 vs. ExPASy TrEMBL
Match: A0A6J1CL37 (2-isopropylmalate synthase OS=Momordica charantia OX=3673 GN=LOC111012138 PE=3 SV=1)

HSP 1 Score: 559.3 bits (1440), Expect = 1.3e-155
Identity = 289/419 (68.97%), Postives = 326/419 (77.80%), Query Frame = 0


Query: 28  ITSCSVVSSIRQRPPYTPNHISDTSYVRVFDTTLRDGEQSPGAALTSKQKFHIARELVKL 87
           I S S  +S  QRPPY PN I D SYVR+FDTTLRDGEQSPGA+LTSK+K  IAR+L KL
Sbjct: 56  IASDSKPNSSVQRPPYIPNRIPDPSYVRIFDTTLRDGEQSPGASLTSKEKLDIARQLAKL 115

Query: 88  GVDAIEAGFPAASRQDFEAVEMIAKEVGNVVDEDGYVPVICGFSRCTESDVRAAWEAVKY 147
           GVD IEAGFPAAS++DFEAV++IAKEVGNVVD+DGYVPVICG SRC E D+R AWEAVKY
Sbjct: 116 GVDIIEAGFPAASKEDFEAVKIIAKEVGNVVDQDGYVPVICGLSRCNEKDIRTAWEAVKY 175

Query: 148 AKRPRIHMFIATSEIHLKHKLRKTRGEVVDIARNMVRFAQSLGCQDVEFSPEDAGRSDKE 207
           AKRPRIH FIATSEIH+K+KLRKTR EV++IAR+MVRFA+SLGC DVEFSPEDAGRSD+E
Sbjct: 176 AKRPRIHTFIATSEIHMKYKLRKTREEVIEIARSMVRFARSLGCDDVEFSPEDAGRSDRE 235

Query: 208 FVYEILGEVIRAGATTVNITDTVGYTVPFEYGNLIADIKSNTHGIHNVIISTHCQNDLGL 267
           F+YEILGEVI+AGATT+NI DTVGYT+P E+G LIADIK+NT GI NVIISTHCQNDLGL
Sbjct: 236 FLYEILGEVIKAGATTLNIPDTVGYTMPVEFGALIADIKTNTPGIENVIISTHCQNDLGL 295

Query: 268 ATANTIA---------------------------VVMTLQCRGDHVLGGLFTGINSRHIF 327
           ATANT+A                           VVM LQCRG+HVLGGL +GINSRHIF
Sbjct: 296 ATANTVAGACAGARQVEVTINGIGERAGNASLEEVVMALQCRGEHVLGGLHSGINSRHIF 355

Query: 328 SASKM-----------------------------DGMLKHKGTYEIIHPEEIGYERSNHA 387
             SKM                             DGMLKHKGTYEII PE+IGYERSN A
Sbjct: 356 LTSKMVEEYTGLNVQPHKAIVGANAFAHESGIHQDGMLKHKGTYEIISPEDIGYERSNDA 415

Query: 388 GIVLGKLSGRHALKSQLRELGYEIENDNFDNIFCHFKAIAQQKKEVTNADLLALISDEV 391
           GIVLGKLSGRHALKS+L+ELGYE++N+N DNIF  FKA+A+QKK VT+ADL AL+SDEV
Sbjct: 416 GIVLGKLSGRHALKSRLQELGYELDNENLDNIFWRFKAVAEQKKRVTDADLRALVSDEV 474

[Close]

BLAST of Lsi03G015110 vs. ExPASy TrEMBL
Match: A0A6J1CKJ3 (2-isopropylmalate synthase OS=Momordica charantia OX=3673 GN=LOC111012138 PE=3 SV=1)

HSP 1 Score: 559.3 bits (1440), Expect = 1.3e-155
Identity = 289/419 (68.97%), Postives = 326/419 (77.80%), Query Frame = 0


Query: 28  ITSCSVVSSIRQRPPYTPNHISDTSYVRVFDTTLRDGEQSPGAALTSKQKFHIARELVKL 87
           I S S  +S  QRPPY PN I D SYVR+FDTTLRDGEQSPGA+LTSK+K  IAR+L KL
Sbjct: 56  IASDSKPNSSVQRPPYIPNRIPDPSYVRIFDTTLRDGEQSPGASLTSKEKLDIARQLAKL 115

Query: 88  GVDAIEAGFPAASRQDFEAVEMIAKEVGNVVDEDGYVPVICGFSRCTESDVRAAWEAVKY 147
           GVD IEAGFPAAS++DFEAV++IAKEVGNVVD+DGYVPVICG SRC E D+R AWEAVKY
Sbjct: 116 GVDIIEAGFPAASKEDFEAVKIIAKEVGNVVDQDGYVPVICGLSRCNEKDIRTAWEAVKY 175

Query: 148 AKRPRIHMFIATSEIHLKHKLRKTRGEVVDIARNMVRFAQSLGCQDVEFSPEDAGRSDKE 207
           AKRPRIH FIATSEIH+K+KLRKTR EV++IAR+MVRFA+SLGC DVEFSPEDAGRSD+E
Sbjct: 176 AKRPRIHTFIATSEIHMKYKLRKTREEVIEIARSMVRFARSLGCDDVEFSPEDAGRSDRE 235

Query: 208 FVYEILGEVIRAGATTVNITDTVGYTVPFEYGNLIADIKSNTHGIHNVIISTHCQNDLGL 267
           F+YEILGEVI+AGATT+NI DTVGYT+P E+G LIADIK+NT GI NVIISTHCQNDLGL
Sbjct: 236 FLYEILGEVIKAGATTLNIPDTVGYTMPVEFGALIADIKTNTPGIENVIISTHCQNDLGL 295

Query: 268 ATANTIA---------------------------VVMTLQCRGDHVLGGLFTGINSRHIF 327
           ATANT+A                           VVM LQCRG+HVLGGL +GINSRHIF
Sbjct: 296 ATANTVAGACAGARQVEVTINGIGERAGNASLEEVVMALQCRGEHVLGGLHSGINSRHIF 355

Query: 328 SASKM-----------------------------DGMLKHKGTYEIIHPEEIGYERSNHA 387
             SKM                             DGMLKHKGTYEII PE+IGYERSN A
Sbjct: 356 LTSKMVEEYTGLNVQPHKAIVGANAFAHESGIHQDGMLKHKGTYEIISPEDIGYERSNDA 415

Query: 388 GIVLGKLSGRHALKSQLRELGYEIENDNFDNIFCHFKAIAQQKKEVTNADLLALISDEV 391
           GIVLGKLSGRHALKS+L+ELGYE++N+N DNIF  FKA+A+QKK VT+ADL AL+SDEV
Sbjct: 416 GIVLGKLSGRHALKSRLQELGYELDNENLDNIFWRFKAVAEQKKRVTDADLRALVSDEV 474

[Close]

BLAST of Lsi03G015110 vs. ExPASy TrEMBL
Match: A0A0A0L7C8 (2-isopropylmalate synthase OS=Cucumis sativus OX=3659 GN=Csa_3G363180 PE=3 SV=1)

HSP 1 Score: 555.8 bits (1431), Expect = 1.4e-154
Identity = 286/419 (68.26%), Postives = 322/419 (76.85%), Query Frame = 0


Query: 28  ITSCSVVSSIRQRPPYTPNHISDTSYVRVFDTTLRDGEQSPGAALTSKQKFHIARELVKL 87
           I S SV  +  +RPPY PN I D SYVRVFDTTLRDGEQSPGA+LT K+K  IAR+L KL
Sbjct: 67  IVSNSVPKASIRRPPYIPNRIPDPSYVRVFDTTLRDGEQSPGASLTVKEKLDIARQLAKL 126

Query: 88  GVDAIEAGFPAASRQDFEAVEMIAKEVGNVVDEDGYVPVICGFSRCTESDVRAAWEAVKY 147
           GVD IEAGFPAAS++DFEAV+MIAKE+GN VDEDGYVPVICG SRC E D+R AWEAVKY
Sbjct: 127 GVDIIEAGFPAASKEDFEAVKMIAKEIGNAVDEDGYVPVICGLSRCNEKDIRTAWEAVKY 186

Query: 148 AKRPRIHMFIATSEIHLKHKLRKTRGEVVDIARNMVRFAQSLGCQDVEFSPEDAGRSDKE 207
           AKRPRIH FIATSEIH++HKLRKT+ EV++IARNMVRFA+SLGC DVEFSPEDAGRSD+E
Sbjct: 187 AKRPRIHTFIATSEIHMEHKLRKTKEEVIEIARNMVRFARSLGCDDVEFSPEDAGRSDRE 246

Query: 208 FVYEILGEVIRAGATTVNITDTVGYTVPFEYGNLIADIKSNTHGIHNVIISTHCQNDLGL 267
           F+Y+ILGEVI+AGATT+NI DTVGYT+PFE+G LIADIKSNT GI NVIIS+HCQNDLGL
Sbjct: 247 FLYQILGEVIKAGATTLNIPDTVGYTMPFEFGKLIADIKSNTPGIENVIISSHCQNDLGL 306

Query: 268 ATANTIA---------------------------VVMTLQCRGDHVLGGLFTGINSRHIF 327
           ATANT+A                           VVM LQCRG+HVLGGL TGINSRHIF
Sbjct: 307 ATANTVAGACAGARQVEVTINGIGERAGNASLEEVVMALQCRGEHVLGGLHTGINSRHIF 366

Query: 328 SASKM-----------------------------DGMLKHKGTYEIIHPEEIGYERSNHA 387
             SKM                             DGMLKHKGTYEI+ PE+IGYERSN A
Sbjct: 367 LTSKMVEEFTGLNVQPHKAIVGANAFAHASGIHQDGMLKHKGTYEIMAPEDIGYERSNDA 426

Query: 388 GIVLGKLSGRHALKSQLRELGYEIENDNFDNIFCHFKAIAQQKKEVTNADLLALISDEV 391
           GIVLGKLSGRHALKS L ELGYE++ +N DN+F  FKA+A+QKK VT+ADL AL+SDEV
Sbjct: 427 GIVLGKLSGRHALKSLLLELGYELDGENLDNVFWRFKAVAEQKKRVTDADLRALVSDEV 485

[Close]

BLAST of Lsi03G015110 vs. NCBI nr
Match: XP_023528730.1 (2-isopropylmalate synthase 2, chloroplastic-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_023528731.1 2-isopropylmalate synthase 2, chloroplastic-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 613.6 bits (1581), Expect = 1.2e-171
Identity = 327/452 (72.35%), Postives = 352/452 (77.88%), Query Frame = 0


Query: 1   MPMSMPMPMPILMPIVSNKYVNISCRPITSCSVVSSIRQRPPYTPNHISDTSYVRVFDTT 60
           MP+SM M  P       N  V IS   ITSC VVSS+RQRPPYTPN+I D SYVRVFDTT
Sbjct: 6   MPVSMSMSKP-------NHDVCISRHQITSCHVVSSVRQRPPYTPNYIPDASYVRVFDTT 65

Query: 61  LRDGEQSPGAALTSKQKFHIARELVKLGVDAIEAGFPAASRQDFEAVEMIAKEVGNVVDE 120
           LRDGEQSPGAALTSKQK  IAR+LVKL VDAIEAGFPAASRQDFEAVEM+AKEVGN V E
Sbjct: 66  LRDGEQSPGAALTSKQKLLIARQLVKLRVDAIEAGFPAASRQDFEAVEMVAKEVGNEVGE 125

Query: 121 DGYVPVICGFSRCTESDVRAAWEAVKYAKRPRIHMFIATSEIHLKHKLRKTRGEVVDIAR 180
           DGYVPVICGFSRCTESDVRAAWEAVKYAKRPRIHMFIATSEIHL HKLRKTRGEVV+IAR
Sbjct: 126 DGYVPVICGFSRCTESDVRAAWEAVKYAKRPRIHMFIATSEIHLTHKLRKTRGEVVEIAR 185

Query: 181 NMVRFAQSLGCQDVEFSPEDAGRSDKEFVYEILGEVIRAGATTVNITDTVGYTVPFEYGN 240
           NMVRFA+SLG  DVEFSPEDAGRSDKEF+Y+ILGEVIRAGATT+NITDTVGYTVP EYG 
Sbjct: 186 NMVRFARSLGFDDVEFSPEDAGRSDKEFLYQILGEVIRAGATTLNITDTVGYTVPAEYGK 245

Query: 241 LIADIKSNTHGIHNVIISTHCQNDLGLATANTIA-------------------------- 300
           LIADIK NT G  NVIISTHCQNDLGLATANTIA                          
Sbjct: 246 LIADIKCNTPGTQNVIISTHCQNDLGLATANTIAGACGGARQVEVTINGIGERAGNASLE 305

Query: 301 -VVMTLQCRGDHVLGGLFTGINSRHIFSASKM---------------------------- 360
            VVMTLQCRG+HVLGGL+TGINSRHIFS SKM                            
Sbjct: 306 EVVMTLQCRGEHVLGGLYTGINSRHIFSTSKMVEEYTGLNVQPHKAIVGANAFAHQSGIH 365

Query: 361 -DGMLKHKGTYEIIHPEEIGYERSNHAGIVLGKLSGRHALKSQLRELGYEIENDNFDNIF 397
            DGMLKH+GTYEII PE+IGY+RSNH GIVLGKLSGRHALKSQLR+LGYEI+ D  DN+F
Sbjct: 366 QDGMLKHRGTYEIIPPEDIGYQRSNHVGIVLGKLSGRHALKSQLRKLGYEIDEDKLDNVF 425

[Close]

BLAST of Lsi03G015110 vs. NCBI nr
Match: KAG6580949.1 (2-isopropylmalate synthase 2, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 594.3 bits (1531), Expect = 7.5e-166
Identity = 310/419 (73.99%), Postives = 335/419 (79.95%), Query Frame = 0


Query: 34  VSSIRQRPPYTPNHISDTSYVRVFDTTLRDGEQSPGAALTSKQKFHIARELVKLGVDAIE 93
           V S+RQRPPYTPN I D SYVRVFDTTLRDGEQSPGAALTSKQK  IAR+LVKL VDAIE
Sbjct: 27  VFSVRQRPPYTPNSIPDASYVRVFDTTLRDGEQSPGAALTSKQKLLIARQLVKLRVDAIE 86

Query: 94  AGFPAASRQDFEAVEMIAKEVGNVVDEDGYVPVICGFSRCTESDVRAAWEAVKYAKRPRI 153
           AGFPAASRQDFEAVEM+AKEVGN V EDGYVPVICGFSRC ESDVRAAWEAVKYAKRPRI
Sbjct: 87  AGFPAASRQDFEAVEMVAKEVGNEVGEDGYVPVICGFSRCIESDVRAAWEAVKYAKRPRI 146

Query: 154 HMFIATSEIHLKHKLRKTRGEVVDIARNMVRFAQSLGCQDVEFSPEDAGRSDKEFVYEIL 213
           HMFIATSEIHL HKLRKTRGEVV+IARNMVRFA+SLG  DVEFSPEDAGRSDKEF+Y+IL
Sbjct: 147 HMFIATSEIHLTHKLRKTRGEVVEIARNMVRFARSLGFDDVEFSPEDAGRSDKEFLYQIL 206

Query: 214 GEVIRAGATTVNITDTVGYTVPFEYGNLIADIKSNTHGIHNVIISTHCQNDLGLATANTI 273
           GEVIRAGATT+NITDTVGYTVP EYG LIADIK NT G  NVIISTHCQNDLGLATANTI
Sbjct: 207 GEVIRAGATTLNITDTVGYTVPAEYGKLIADIKCNTPGTQNVIISTHCQNDLGLATANTI 266

Query: 274 A---------------------------VVMTLQCRGDHVLGGLFTGINSRHIFSASKM- 333
           A                           VVMTL+CRG+HVLGGL+TGINSRHIFSASKM 
Sbjct: 267 AGACGGARQVEVTINGIGERAGNASLEEVVMTLRCRGEHVLGGLYTGINSRHIFSASKMV 326

Query: 334 ----------------------------DGMLKHKGTYEIIHPEEIGYERSNHAGIVLGK 393
                                       DGMLKH+GTYEII PE+IGY+RSNH GIVLGK
Sbjct: 327 EEYTGLNVQPHKAIVGANAFAHQSGIHQDGMLKHRGTYEIIPPEDIGYQRSNHVGIVLGK 386

Query: 394 LSGRHALKSQLRELGYEIENDNFDNIFCHFKAIAQQKKEVTNADLLALISDEVSKKIDI 397
           LSGRHAL+S LR+LGY+I+ D FDN+F  FKA+A+QKK V +ADL+AL+SDEVSKK+DI
Sbjct: 387 LSGRHALRSHLRKLGYKIDEDKFDNVFWRFKALAEQKKRVMDADLIALVSDEVSKKVDI 445

[Close]

BLAST of Lsi03G015110 vs. NCBI nr
Match: XP_022934697.1 (2-isopropylmalate synthase 2, chloroplastic-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 593.2 bits (1528), Expect = 1.7e-165
Identity = 319/452 (70.58%), Postives = 345/452 (76.33%), Query Frame = 0


Query: 1   MPMSMPMPMPILMPIVSNKYVNISCRPITSCSVVSSIRQRPPYTPNHISDTSYVRVFDTT 60
           MP+SM M M        N  V IS   ITSC VVSS+RQRPPYTPN I D  YVRVFDTT
Sbjct: 6   MPVSMSMSMS-----KPNHDVCISRHQITSCHVVSSVRQRPPYTPNSILDAPYVRVFDTT 65

Query: 61  LRDGEQSPGAALTSKQKFHIARELVKLGVDAIEAGFPAASRQDFEAVEMIAKEVGNVVDE 120
           LRDGEQSPG ALT KQK  IAR+LVKL VDAIEAGFPAASRQDFEAVEM+AKEVGN V E
Sbjct: 66  LRDGEQSPGTALTLKQKLLIARQLVKLRVDAIEAGFPAASRQDFEAVEMVAKEVGNEVGE 125

Query: 121 DGYVPVICGFSRCTESDVRAAWEAVKYAKRPRIHMFIATSEIHLKHKLRKTRGEVVDIAR 180
           DGYVPVICGFSRCTESDVRAAWEAVKYAKRPRIHMFIATSEIHL HKLRKTRGEVV+IAR
Sbjct: 126 DGYVPVICGFSRCTESDVRAAWEAVKYAKRPRIHMFIATSEIHLTHKLRKTRGEVVEIAR 185

Query: 181 NMVRFAQSLGCQDVEFSPEDAGRSDKEFVYEILGEVIRAGATTVNITDTVGYTVPFEYGN 240
           NMVRFA+SLG  DVEFSPEDAGRSDKEF+Y+ILGEVIRAGATT+NITDTVGYTVP EYG 
Sbjct: 186 NMVRFARSLGFDDVEFSPEDAGRSDKEFLYQILGEVIRAGATTLNITDTVGYTVPAEYGK 245

Query: 241 LIADIKSNTHGIHNVIISTHCQNDLGLATANTIA-------------------------- 300
           LIADIK NT G  NVIISTHCQNDLGLATANTIA                          
Sbjct: 246 LIADIKCNTPGTQNVIISTHCQNDLGLATANTIAGACGGARQVEVTINGIGEKAGNASLE 305

Query: 301 -VVMTLQCRGDHVLGGLFTGINSRHIFSASKM---------------------------- 360
            +VMTL+CRG+HV GGL+TGINSRHIFSASK+                            
Sbjct: 306 EMVMTLRCRGEHVFGGLYTGINSRHIFSASKIVEEYTGLNVQPHKAIVGANAFAHQSGIH 365

Query: 361 -DGMLKHKGTYEIIHPEEIGYERSNHAGIVLGKLSGRHALKSQLRELGYEIENDNFDNIF 397
            DGMLKH+GTYEII PE+IGY+RSNH  IVLGKLSGRHALKS LR+LGYEI+ D  DN+F
Sbjct: 366 QDGMLKHRGTYEIISPEDIGYQRSNHVVIVLGKLSGRHALKSYLRKLGYEIDEDKLDNVF 425

[Close]

BLAST of Lsi03G015110 vs. NCBI nr
Match: XP_022934698.1 (2-isopropylmalate synthase 2, chloroplastic-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 567.8 bits (1462), Expect = 7.5e-158
Identity = 305/433 (70.44%), Postives = 328/433 (75.75%), Query Frame = 0


Query: 1   MPMSMPMPMPILMPIVSNKYVNISCRPITSCSVVSSIRQRPPYTPNHISDTSYVRVFDTT 60
           MP+SM M M        N  V IS   ITSC VVSS+RQRPPYTPN I D  YVRVFDTT
Sbjct: 6   MPVSMSMSMS-----KPNHDVCISRHQITSCHVVSSVRQRPPYTPNSILDAPYVRVFDTT 65

Query: 61  LRDGEQSPGAALTSKQKFHIARELVKLGVDAIEAGFPAASRQDFEAVEMIAKEVGNVVDE 120
           LRDGEQSPG ALT KQK  IAR+LVKL VDAIEAGFPAASRQDFEAVEM+AKEVGN V E
Sbjct: 66  LRDGEQSPGTALTLKQKLLIARQLVKLRVDAIEAGFPAASRQDFEAVEMVAKEVGNEVGE 125

Query: 121 DGYVPVICGFSRCTESDVRAAWEAVKYAKRPRIHMFIATSEIHLKHKLRKTRGEVVDIAR 180
           DGYVPVICGFSRCTESDVRAAWEAVKYAKRPRIHMFIATSEIHL HKLRKTRGEVV+IAR
Sbjct: 126 DGYVPVICGFSRCTESDVRAAWEAVKYAKRPRIHMFIATSEIHLTHKLRKTRGEVVEIAR 185

Query: 181 NMVRFAQSLGCQDVEFSPEDAGRSDKEFVYEILGEVIRAGATTVNITDTVGYTVPFEYGN 240
           NMVRFA+SLG  DVEFSPEDAGRSDKEF+Y+ILGEVIRAGATT+NITDTVGYTVP EYG 
Sbjct: 186 NMVRFARSLGFDDVEFSPEDAGRSDKEFLYQILGEVIRAGATTLNITDTVGYTVPAEYGK 245

Query: 241 LIADIKSNTHGIHNVIISTHCQNDLGLATANTIA-------------------------- 300
           LIADIK NT G  NVIISTHCQNDLGLATANTIA                          
Sbjct: 246 LIADIKCNTPGTQNVIISTHCQNDLGLATANTIAGACGGARQVEVTINGIGEKAGNASLE 305

Query: 301 -VVMTLQCRGDHVLGGLFTGINSRHIFSASKM---------------------------- 360
            +VMTL+CRG+HV GGL+TGINSRHIFSASK+                            
Sbjct: 306 EMVMTLRCRGEHVFGGLYTGINSRHIFSASKIVEEYTGLNVQPHKAIVGANAFAHQSGIH 365

Query: 361 -DGMLKHKGTYEIIHPEEIGYERSNHAGIVLGKLSGRHALKSQLRELGYEIENDNFDNIF 378
            DGMLKH+GTYEII PE+IGY+RSNH  IVLGKLSGRHALKS LR+LGYEI+ D  DN+F
Sbjct: 366 QDGMLKHRGTYEIISPEDIGYQRSNHVVIVLGKLSGRHALKSYLRKLGYEIDEDKLDNVF 425

[Close]

BLAST of Lsi03G015110 vs. NCBI nr
Match: XP_038875433.1 (2-isopropylmalate synthase 2, chloroplastic-like [Benincasa hispida])

HSP 1 Score: 559.7 bits (1441), Expect = 2.0e-155
Identity = 287/419 (68.50%), Postives = 328/419 (78.28%), Query Frame = 0


Query: 28  ITSCSVVSSIRQRPPYTPNHISDTSYVRVFDTTLRDGEQSPGAALTSKQKFHIARELVKL 87
           +++  + SSIR RPPY PN I D SYVR+FDTTLRDGEQSPGA+LT+K+K  IAR+L KL
Sbjct: 65  VSNSGLKSSIR-RPPYIPNRIPDPSYVRIFDTTLRDGEQSPGASLTAKEKLDIARQLAKL 124

Query: 88  GVDAIEAGFPAASRQDFEAVEMIAKEVGNVVDEDGYVPVICGFSRCTESDVRAAWEAVKY 147
           GVD IEAGFPAAS++DFEAV+MIAKEVGN VDEDGYVPVICG SRC E+D+R AWEAVKY
Sbjct: 125 GVDIIEAGFPAASKEDFEAVKMIAKEVGNAVDEDGYVPVICGLSRCNENDIRTAWEAVKY 184

Query: 148 AKRPRIHMFIATSEIHLKHKLRKTRGEVVDIARNMVRFAQSLGCQDVEFSPEDAGRSDKE 207
           AKRPRIH FIATSEIH++HKLRKTR +V++IARNMVRFA+SLGC DVEFSPEDAGRSD+E
Sbjct: 185 AKRPRIHTFIATSEIHMEHKLRKTREQVIEIARNMVRFARSLGCDDVEFSPEDAGRSDRE 244

Query: 208 FVYEILGEVIRAGATTVNITDTVGYTVPFEYGNLIADIKSNTHGIHNVIISTHCQNDLGL 267
           F+Y+ILGEVI+AGATT+NI DTVGYT+P E+G LIADIKSNT GI NVIISTHCQNDLGL
Sbjct: 245 FLYQILGEVIKAGATTLNIPDTVGYTMPVEFGKLIADIKSNTPGIENVIISTHCQNDLGL 304

Query: 268 ATANTIA---------------------------VVMTLQCRGDHVLGGLFTGINSRHIF 327
           ATANT+A                           VVM+LQCRG+HVLGGL TGINSRHIF
Sbjct: 305 ATANTVAGACAGARQVEVTINGIGERAGNASLEEVVMSLQCRGEHVLGGLHTGINSRHIF 364

Query: 328 SASKM-----------------------------DGMLKHKGTYEIIHPEEIGYERSNHA 387
             SKM                             DGMLKHKGTYEII PE+IGYERSN A
Sbjct: 365 LTSKMVEEYTGLNVQPHKAIVGANAFAHESGIHQDGMLKHKGTYEIISPEDIGYERSNEA 424

Query: 388 GIVLGKLSGRHALKSQLRELGYEIENDNFDNIFCHFKAIAQQKKEVTNADLLALISDEV 391
           GIVLGKLSGRHALKS+L ELGYE++++N DN+F  FKA+A+QKK VT+ADL AL+SDEV
Sbjct: 425 GIVLGKLSGRHALKSRLLELGYELDSENLDNVFWRFKAVAEQKKRVTDADLRALVSDEV 482

[Close]

BLAST of Lsi03G015110 vs. TAIR 10
Match: AT1G74040.1 (2-isopropylmalate synthase 1 )

HSP 1 Score: 518.1 bits (1333), Expect = 6.4e-147
Identity = 263/409 (64.30%), Postives = 310/409 (75.79%), Query Frame = 0


Query: 38  RQRPPYTPNHISDTSYVRVFDTTLRDGEQSPGAALTSKQKFHIARELVKLGVDAIEAGFP 97
           R+RP Y PN ISD +YVR+FDTTLRDGEQSPGA LTSK+K  IAR+L KLGVD IEAGFP
Sbjct: 71  RRRPNYIPNRISDPNYVRIFDTTLRDGEQSPGATLTSKEKLDIARQLAKLGVDIIEAGFP 130

Query: 98  AASRQDFEAVEMIAKEVGNVVDEDGYVPVICGFSRCTESDVRAAWEAVKYAKRPRIHMFI 157
           AAS+ DFEAV+ IA+ VGN VDE+GYVPVICG SRC + D+  AWEAVKYAKRPRIH FI
Sbjct: 131 AASKDDFEAVKTIAETVGNTVDENGYVPVICGLSRCNKKDIETAWEAVKYAKRPRIHTFI 190

Query: 158 ATSEIHLKHKLRKTRGEVVDIARNMVRFAQSLGCQDVEFSPEDAGRSDKEFVYEILGEVI 217
           ATS+IHLK+KL+K++ EV++IARNMVRFA+SLGC+DVEFSPEDAGRS++E++YEILGEVI
Sbjct: 191 ATSDIHLKYKLKKSKEEVIEIARNMVRFARSLGCEDVEFSPEDAGRSEREYLYEILGEVI 250

Query: 218 RAGATTVNITDTVGYTVPFEYGNLIADIKSNTHGIHNVIISTHCQNDLGLATANTIA--- 277
           +AGATT+NI DTVG T+P E+G LIADIK+NT GI NVIISTHCQNDLGL+TANT++   
Sbjct: 251 KAGATTLNIPDTVGITLPSEFGQLIADIKANTPGIQNVIISTHCQNDLGLSTANTLSGAH 310

Query: 278 ------------------------VVMTLQCRGDHVLGGLFTGINSRHIFSASKM----- 337
                                   VVM ++CRGDHVLGGLFTGI++RHI   SKM     
Sbjct: 311 SGARQVEVTINGIGERAGNASLEEVVMAIKCRGDHVLGGLFTGIDTRHIVMTSKMVEEYT 370

Query: 338 ------------------------DGMLKHKGTYEIIHPEEIGYERSNHAGIVLGKLSGR 391
                                   DGMLKHKGTYEI+ PEEIG ERSN AGIVLGKLSGR
Sbjct: 371 GMQTQPHKAIVGANAFAHESGIHQDGMLKHKGTYEIMSPEEIGLERSNDAGIVLGKLSGR 430

[Close]

BLAST of Lsi03G015110 vs. TAIR 10
Match: AT1G18500.1 (methylthioalkylmalate synthase-like 4 )

HSP 1 Score: 508.1 bits (1307), Expect = 6.6e-144
Identity = 268/454 (59.03%), Postives = 324/454 (71.37%), Query Frame = 0


Query: 4   SMPMPMPILMPIVSNKYVNISCRPIT---SCSVV--------SSIRQRPPYTPNHISDTS 63
           S P P+        + + +IS R  T   SCS+         +  R RP Y PN ISD +
Sbjct: 28  SKPTPISSSFRFQPSHHRSISLRSQTLRLSCSISDPSPLPPHTPRRPRPEYIPNRISDPN 87

Query: 64  YVRVFDTTLRDGEQSPGAALTSKQKFHIARELVKLGVDAIEAGFPAASRQDFEAVEMIAK 123
           YVRVFDTTLRDGEQSPGA LTSK+K  IAR+L KLGVD IEAGFPAAS+ DFEAV+ IA+
Sbjct: 88  YVRVFDTTLRDGEQSPGATLTSKEKLDIARQLAKLGVDIIEAGFPAASKDDFEAVKTIAE 147

Query: 124 EVGNVVDEDGYVPVICGFSRCTESDVRAAWEAVKYAKRPRIHMFIATSEIHLKHKLRKTR 183
            VGN VDE+GYVPVICG SRC + D+  AW+AVKYAKRPRIH FIATS+IHL++KL+KT+
Sbjct: 148 TVGNTVDENGYVPVICGLSRCNKKDIERAWDAVKYAKRPRIHTFIATSDIHLEYKLKKTK 207

Query: 184 GEVVDIARNMVRFAQSLGCQDVEFSPEDAGRSDKEFVYEILGEVIRAGATTVNITDTVGY 243
            EV++IAR+MVRFA+SLGC+DVEFSPEDAGRS++E++YEILGEVI+AGATT+NI DTVG 
Sbjct: 208 AEVIEIARSMVRFARSLGCEDVEFSPEDAGRSEREYLYEILGEVIKAGATTLNIPDTVGI 267

Query: 244 TVPFEYGNLIADIKSNTHGIHNVIISTHCQNDLGLATANTIA------------------ 303
           T+P E+G LI D+K+NT GI NV+ISTHCQNDLGL+TANT++                  
Sbjct: 268 TLPSEFGQLITDLKANTPGIENVVISTHCQNDLGLSTANTLSGAHAGARQMEVTINGIGE 327

Query: 304 ---------VVMTLQCRGDHVLGGLFTGINSRHIFSASKM-------------------- 363
                    VVM ++CRGDHVLGGLFTGI++RHI   SKM                    
Sbjct: 328 RAGNASLEEVVMAIKCRGDHVLGGLFTGIDTRHIVMTSKMVEEYTGMQTQPHKAIVGANA 387

Query: 364 ---------DGMLKHKGTYEIIHPEEIGYERSNHAGIVLGKLSGRHALKSQLRELGYEIE 391
                    DGMLKHKGTYEII PEEIG ERSN AGIVLGKLSGRHALK +L ELGY+++
Sbjct: 388 FAHESGIHQDGMLKHKGTYEIICPEEIGLERSNDAGIVLGKLSGRHALKDRLTELGYQLD 447

[Close]

BLAST of Lsi03G015110 vs. TAIR 10
Match: AT5G23020.1 (2-isopropylmalate synthase 2 )

HSP 1 Score: 391.7 bits (1005), Expect = 6.9e-109
Identity = 201/428 (46.96%), Postives = 282/428 (65.89%), Query Frame = 0


Query: 23  ISCRPITSCSVVSS-------IRQRPPYTPNHISDTSYVRVFDTTLRDGEQSPGAALTSK 82
           +SC    S  V +S       + +RP Y PN +   +YVRV DTTLRDGEQSPGAALT  
Sbjct: 47  VSCCSAESKKVATSATDLKPIMERRPEYIPNKLPHKNYVRVLDTTLRDGEQSPGAALTPP 106

Query: 83  QKFHIARELVKLGVDAIEAGFPAASRQDFEAVEMIAKEVGNVVDED-GYVPVICGFSRCT 142
           QK  IAR+L KL VD +E GFP +S ++FEA++ IAK VGN VDE+ GYVPVICG +RC 
Sbjct: 107 QKLEIARQLAKLRVDIMEVGFPVSSEEEFEAIKTIAKTVGNEVDEETGYVPVICGIARCK 166

Query: 143 ESDVRAAWEAVKYAKRPRIHMFIATSEIHLKHKLRKTRGEVVDIARNMVRFAQSLGCQDV 202
           + D+ A WEA+KYAKRPR+ +F +TSEIH+K+KL+KT+ EV+++A N V++A+SLG +D+
Sbjct: 167 KRDIEATWEALKYAKRPRVMLFTSTSEIHMKYKLKKTKEEVIEMAVNSVKYAKSLGFKDI 226

Query: 203 EFSPEDAGRSDKEFVYEILGEVIRAGATTVNITDTVGYTVPFEYGNLIADIKSNTHGIHN 262
           +F  ED GR++K+F+ +ILGE I+AGATTV   DTVG  +P E+G L+A +  NT G  +
Sbjct: 227 QFGCEDGGRTEKDFICKILGESIKAGATTVGFADTVGINMPQEFGELVAYVIENTPGADD 286

Query: 263 VIISTHCQNDLGLATANTIA---------------------------VVMTLQCRGDHVL 322
           ++ + HC NDLG+ATANTI+                           VVM L+CRG+ ++
Sbjct: 287 IVFAIHCHNDLGVATANTISGICAGARQVEVTINGIGERSGNAPLEEVVMALKCRGESLM 346

Query: 323 GGLFTGINSRHIFSASKM-----------------------------DGMLKHKGTYEII 382
            G++T I+SR I + SKM                             DG+LK++ TYEI+
Sbjct: 347 DGVYTKIDSRQIMATSKMVQEHTGMYVQPHKPIVGDNCFVHESGIHQDGILKNRSTYEIL 406

Query: 383 HPEEIGYERSNHAGIVLGKLSGRHALKSQLRELGYEIENDNFDNIFCHFKAIAQQKKEVT 387
            PE++G  +S ++GIVLGKLSGRHA+K +L+ELGYEI ++ F++IF  ++ + + KK +T
Sbjct: 407 SPEDVGIVKSENSGIVLGKLSGRHAVKDRLKELGYEISDEKFNDIFSRYRELTKDKKRIT 466

[Close]

BLAST of Lsi03G015110 vs. TAIR 10
Match: AT5G23010.1 (methylthioalkylmalate synthase 1 )

HSP 1 Score: 390.2 bits (1001), Expect = 2.0e-108
Identity = 203/430 (47.21%), Postives = 284/430 (66.05%), Query Frame = 0


Query: 22  NISCRPITSCSVVSSIRQR-PPYTPNHISDTSYVRVFDTTLRDGEQSPGAALTSKQKFHI 81
           ++S    TS + +  + +R P Y PN + D +YVRVFDTTLRDGEQSPG +LT  QK  I
Sbjct: 52  SVSKNAATSSTDLKPVVERWPEYIPNKLPDGNYVRVFDTTLRDGEQSPGGSLTPPQKLEI 111

Query: 82  ARELVKLGVDAIEAGFPAASRQDFEAVEMIAKEVGNVVDED-GYVPVICGFSRCTESDVR 141
           AR+L KL VD +E GFP +S ++ E ++ IAK VGN VDE+ GYVPVIC  +RC   D+ 
Sbjct: 112 ARQLAKLRVDIMEVGFPGSSEEELETIKTIAKTVGNEVDEETGYVPVICAIARCKHRDIE 171

Query: 142 AAWEAVKYAKRPRIHMFIATSEIHLKHKLRKTRGEVVDIARNMVRFAQSLGCQDVEFSPE 201
           A WEA+KYAKRPRI +F +TS+IH+K+KL+KT+ EV+++A + +RFA+SLG  D++F  E
Sbjct: 172 ATWEALKYAKRPRILVFTSTSDIHMKYKLKKTQEEVIEMAVSSIRFAKSLGFNDIQFGCE 231

Query: 202 DAGRSDKEFVYEILGEVIRAGATTVNITDTVGYTVPFEYGNLIADIKSNTHGIHNVIIST 261
           D GRSDK+F+ +ILGE I+AG T V I DTVG  +P EYG L+  +K+NT GI +V+++ 
Sbjct: 232 DGGRSDKDFLCKILGEAIKAGVTVVTIGDTVGINMPHEYGELVTYLKANTPGIDDVVVAV 291

Query: 262 HCQNDLGLATANTIA---------------------------VVMTLQCRGDHVLGGLFT 321
           HC NDLGLATAN+IA                           VVM L+CRG +V+ G++T
Sbjct: 292 HCHNDLGLATANSIAGIRAGARQVEVTINGIGERSGNASLEEVVMALKCRGAYVINGVYT 351

Query: 322 GINSRHIFSASKM-----------------------------DGMLKHKGTYEIIHPEEI 381
            I++R I + SKM                             DG+LK++ TYEI+ PE+I
Sbjct: 352 KIDTRQIMATSKMVQEYTGLYVQAHKPIVGANCFVHESGIHQDGILKNRSTYEILSPEDI 411

Query: 382 GYERSNHAGIVLGKLSGRHALKSQLRELGYEIENDNFDNIFCHFKAIAQQKKEVTNADLL 392
           G  +S ++G+VLGKLSGRHA+K +L+ELGYE++++  + +F  F+ + + KK +T+ADL 
Sbjct: 412 GIVKSQNSGLVLGKLSGRHAVKDRLKELGYELDDEKLNAVFSLFRDLTKNKKRITDADLK 471

The following BLAST results are available for this feature:

BLAST of Lsi03G015110 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9C550	8.9e-146	64.30	2-isopropylmalate synthase 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=I...	[more]
Q9LPR4	9.3e-143	59.03	2-isopropylmalate synthase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=I...	[more]
O04973	8.4e-136	57.18	2-isopropylmalate synthase A OS=Solanum pennellii OX=28526 GN=IPMSA PE=2 SV=1	[more]
O04974	3.1e-130	56.90	2-isopropylmalate synthase B OS=Solanum pennellii OX=28526 GN=IPMSB PE=2 SV=1	[more]
Q39891	3.5e-126	57.92	Probable 2-isopropylmalate synthase OS=Glycine max OX=3847 GN=GMN56 PE=2 SV=1	[more]

BLAST of Lsi03G015110 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1F3I8	8.1e-166	70.58	2-isopropylmalate synthase 2, chloroplastic-like isoform X1 OS=Cucurbita moschat...	[more]
A0A6J1F8D9	3.6e-158	70.44	2-isopropylmalate synthase 2, chloroplastic-like isoform X2 OS=Cucurbita moschat...	[more]
A0A6J1CL37	1.3e-155	68.97	2-isopropylmalate synthase OS=Momordica charantia OX=3673 GN=LOC111012138 PE=3 S...	[more]
A0A6J1CKJ3	1.3e-155	68.97	2-isopropylmalate synthase OS=Momordica charantia OX=3673 GN=LOC111012138 PE=3 S...	[more]
A0A0A0L7C8	1.4e-154	68.26	2-isopropylmalate synthase OS=Cucumis sativus OX=3659 GN=Csa_3G363180 PE=3 SV=1	[more]

BLAST of Lsi03G015110 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023528730.1	1.2e-171	72.35	2-isopropylmalate synthase 2, chloroplastic-like isoform X1 [Cucurbita pepo subs...	[more]
KAG6580949.1	7.5e-166	73.99	2-isopropylmalate synthase 2, chloroplastic, partial [Cucurbita argyrosperma sub...	[more]
XP_022934697.1	1.7e-165	70.58	2-isopropylmalate synthase 2, chloroplastic-like isoform X1 [Cucurbita moschata]	[more]
XP_022934698.1	7.5e-158	70.44	2-isopropylmalate synthase 2, chloroplastic-like isoform X2 [Cucurbita moschata]	[more]
XP_038875433.1	2.0e-155	68.50	2-isopropylmalate synthase 2, chloroplastic-like [Benincasa hispida]	[more]

BLAST of Lsi03G015110 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G74040.1	6.4e-147	64.30	2-isopropylmalate synthase 1	[more]
AT1G18500.1	6.6e-144	59.03	methylthioalkylmalate synthase-like 4	[more]
AT5G23020.1	6.9e-109	46.96	2-isopropylmalate synthase 2	[more]
AT5G23010.1	2.0e-108	47.21	methylthioalkylmalate synthase 1	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR013785	Aldolase-type TIM barrel	GENE3D	3.20.20.70	Aldolase class I	coord: 34..279 e-value: 5.1E-76 score: 258.0
None	No IPR available	GENE3D	1.10.238.260		coord: 292..398 e-value: 2.0E-20 score: 75.2
None	No IPR available	PANTHER	PTHR10277	HOMOCITRATE SYNTHASE-RELATED	coord: 306..395 coord: 32..273
None	No IPR available	PANTHER	PTHR10277:SF74	2-ISOPROPYLMALATE SYNTHASE-RELATED	coord: 306..395 coord: 32..273
None	No IPR available	PANTHER	PTHR10277	HOMOCITRATE SYNTHASE-RELATED	coord: 275..305
None	No IPR available	PANTHER	PTHR10277:SF74	2-ISOPROPYLMALATE SYNTHASE-RELATED	coord: 275..305
None	No IPR available	CDD	cd07940	DRE_TIM_IPMS	coord: 56..305 e-value: 2.21207E-132 score: 378.715
None	No IPR available	SUPERFAMILY	51569	Aldolase	coord: 50..277
IPR000891	Pyruvate carboxyltransferase	PFAM	PF00682	HMGL-like	coord: 54..276 e-value: 3.0E-69 score: 233.5
IPR000891	Pyruvate carboxyltransferase	PROSITE	PS50991	PYR_CT	coord: 54..324 score: 25.597393
IPR002034	Alpha-isopropylmalate/homocitrate synthase, conserved site	PROSITE	PS00815	AIPM_HOMOCIT_SYNTH_1	coord: 61..77

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi03G015110.1	Lsi03G015110.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0009098	leucine biosynthetic process
biological_process	GO:0019752	carboxylic acid metabolic process
cellular_component	GO:0009507	chloroplast
molecular_function	GO:0003852	2-isopropylmalate synthase activity
molecular_function	GO:0046912	acyltransferase, acyl groups converted into alkyl on transfer
molecular_function	GO:0003824	catalytic activity